title: Impact of Sod on the expression of stress-related genes in Listeria monocytogenes 4b G with/without paraquat treatment.
abstract: Listeria monocytogenes is a foodborne pathogen that causes listeriosis. Paraquat can generate reactive oxygen species (ROS) in cells, which results in oxidative stress. It was first shown that 1 mM of paraquat inhibited the growth rate of a superoxide dismutase (sod)-deletion mutant (∆sod) generated from L. monocytogenes 4b G but not in the wild-type, and induced the expression of other resistance genes (kat, fri, perR, sigB, and recA) as well as sod in the wild type. Interestingly, without paraquat treatment the expression of all the 5 genes were repressed in ∆sod compared to the wild type, while the expression of recA triggering SOS response, a global response to DNA damage, was increased in ∆sod in the presence of 1 mM paraquat. Taken together, these results suggest that SOD plays a central role in oxidant defense of L. monocytogenes 4b G, and SOS probably significantly impacts ∆sod survival under oxidative stress.
authors.affiliation: MOST-USDA Joint Research Center for Food Safety, School of Agriculture and Biology, and State Key Lab of Microbial Metabolism, Shanghai Jiao Tong Univ, Shanghai, 200240, China.
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Suo
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Zhou
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Huang
authors.forename: Chunlei
authors.initials: C
authors.lastname: Shi
authors.forename: Karl
authors.initials: K
authors.lastname: Matthews
authors.forename: Xianming
authors.initials: X
authors.lastname: Shi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D055786:Gene Knockout Techniques; D008089:Listeria monocytogenes / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D050296:Microbial Viability / Q000187:drug effects; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress; D010269:Paraquat / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D016877:Oxidants; D013482:Superoxide Dismutase; D010269:Paraquat
keywords: Listeria monocytogenes; antioxidant capacity; methyl viologen; oxidative stress; real-time quantitative polymerase chain reaction (RT-qPCR)