title: Evaluation of glycophenotype in prostatic neoplasm by chemiluminescent assay.
abstract: This work aimed to evaluate the glycophenotype in normal prostate, bening prostatic hyperplasia (BPH) and prostatic adenocarcinoma (PCa) tissues by a chemiluminescent method. Concanavalin A (Con A), Ulex europaeus agglutinin (UEA-I) and Peanut agglutinin (PNA) lectins were conjugated to acridinium ester (lectins-AE). These conjugates remained capable to recognize their specific carbohydrates. Tissue samples were incubated with lectins-AE. The chemiluminescence of the tissue-lectin-AE complex was expressed in relative light units (RLU). Transformed tissues (0.25 cm(2) by 8 µm of thickness) showed statistical significant lower α-D-glucose/mannose (BPH: 226,931 ± 17,436; PCa: 239,520 ± 12,398) and Gal-β(1-3)-GalNAc (BPH: 28,754 ± 2,157; PCa: 16,728 ± 1,204) expression than normal tissues (367,566 ± 48,550 and 409,289 ± 22,336, respectively). However, higher α-L-fucose expression was observed in PCa (251,118 ± 14,193) in relation to normal (200,979 ± 21,318) and BHP (169,758 ± 10,264) tissues. It was observed an expressive decreasing of the values of RLU by inhibition of the interaction between tissues and lectins-AE using their specific carbohydrates. The relationship between RLU and tissue area showed a linear correlation for all lectin-AE in both transformed tissues. These results indicated that the used method is an efficient tool for specific, sensitive and quantitative analyses of prostatic glycophenotype.
authors.affiliation: Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Cidade Universitária Recife, Pernambuco, Brasil.
authors.forename: Lúcia Patrícia Bezerra Gomes
authors.initials: LP
authors.lastname: da Silva
authors.affiliation: Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Cidade Universitária Recife, Pernambuco, Brasil ; Faculdade de Ciências, Educação e Tecnologia de Garanhuns (FACETEG), Universidade de Pernambuco (UPE) Garanhuns, Pernambuco, Brasil.
authors.forename: Sinara Mônica Vitalino
authors.initials: SM
authors.lastname: de Almeida
authors.affiliation: Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Cidade Universitária Recife, Pernambuco, Brasil.
authors.forename: Luiza Rayanna Amorim
authors.initials: LR
authors.lastname: de Lima
authors.affiliation: Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Cidade Universitária Recife, Pernambuco, Brasil.
authors.forename: Carmelita de Lima Bezerra
authors.initials: Cde L
authors.lastname: Cavalcanti
authors.affiliation: Departamento de Patologia e Hospital das clínicas, Universidade Federal de Pernambuco, Cidade Universitária Recife, Pernambuco, Brasil.
authors.forename: Mariana Montenegro
authors.initials: MM
authors.lastname: de Melo Lira
authors.affiliation: Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Cidade Universitária Recife, Pernambuco, Brasil.
authors.forename: Maria da Paz Carvalho
authors.initials: Mda P
authors.lastname: da Silva
authors.affiliation: Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Cidade Universitária Recife, Pernambuco, Brasil.
authors.forename: Eduardo Isidoro Carneiro
authors.initials: EI
authors.lastname: Beltrão
authors.affiliation: Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Cidade Universitária Recife, Pernambuco, Brasil.
authors.forename: Luiz Bezerra
authors.initials: LB
authors.lastname: de Carvalho Júnior
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis* / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D011470:Prostatic Hyperplasia / Q000378:metabolism / Q000473:pathology; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D002241:Carbohydrates; D037102:Lectins
keywords: Chemiluminescence; benign prostatic hyperplasia; carbohydrates; lectins; prostatic cancer