title: Steroids and antihistamines synergize to inhibit rat's airway smooth muscle contractility.
abstract: Both glucocorticoids and H1-antihistamines were widely used on patients with allergic rhinitis (AR) and obstructive airway diseases. However, their direct effects on airway smooth muscle were not fully explored. In this study, we tested the effectiveness of prednisolone (Kidsolone) and levocetirizine (Xyzal) on isolated rat trachea submersed in Kreb's solution in a muscle bath. Changes in tracheal contractility in response to the application of parasympathetic mimetic agents were measured. The following assessments of the drug were performed: (1) effect on tracheal smooth muscle resting tension; (2) effect on contraction caused by 10(-6) M methacholine; (3) effect of the drug on electrical field stimulation (EFS) induced tracheal smooth muscle contractions. The result revealed sole use of Kidsolone or Xyzal elicited no significant effect or only a little relaxation response on tracheal tension after methacholine treatment. The tension was 90.5 ± 7.5 and 99.5 ± 0.8 % at 10(-4) M for Xyzal and 10(-5) M for Kidsolone, respectively. However, a dramatically spasmolytic effect was observed after co-administration of Kidsolone and Xyzal and the tension dropped to 67.5 ± 13.6 %, with statistical significance (p < 0.05). As for EFS-induced contractions, Kidsolone had no direct effect but Xyzal could inhibit it, with increasing basal tension. In conclusion, using glucocorticoids alone had no spasmolytic effect but they can be synergized with antihistamines to dramatically relax the trachea smooth muscle within minutes. Therefore, for AR patients with acute asthma attack, combined use of those two drugs is recommended.
authors.affiliation: Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan, Republic of China, m871435@ndmctsgh.edu.tw.
authors.forename: Shao-Cheng
authors.initials: SC
authors.lastname: Liu
authors.forename: Yueng-Hsiang
authors.initials: YH
authors.lastname: Chu
authors.forename: Chuan-Hsiang
authors.initials: CH
authors.lastname: Kao
authors.forename: Chi-Chung
authors.initials: CC
authors.lastname: Wu
authors.forename: Hsing-Won
authors.initials: HW
authors.lastname: Wang
mesh_terms: D000818:Animals; D017332:Cetirizine / Q000494:pharmacology*; D018678:Cholinergic Agents; D004195:Disease Models, Animal; D004357:Drug Synergism; D004558:Electric Stimulation / Q000379:methods; D005938:Glucocorticoids / Q000494:pharmacology; D006633:Histamine Antagonists / Q000494:pharmacology; D006801:Humans; D008173:Lung Diseases, Obstructive / Q000188:drug therapy* / Q000503:physiopathology; D008297:Male; D016210:Methacholine Chloride / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D011239:Prednisolone / Q000494:pharmacology*; D051381:Rats; D065631:Rhinitis, Allergic / Q000188:drug therapy* / Q000503:physiopathology; D014132:Trachea / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D016896:Treatment Outcome
chemical_list: D018678:Cholinergic Agents; D005938:Glucocorticoids; D006633:Histamine Antagonists; D016210:Methacholine Chloride; C472067:levocetirizine; D011239:Prednisolone; D017332:Cetirizine