title: Synergistic proteins for the enhanced enzymatic hydrolysis of cellulose by cellulase.
abstract: Reducing the enzyme loadings for enzymatic saccharification of lignocellulose is required for economically feasible production of biofuels and biochemicals. One strategy is addition of small amounts of synergistic proteins to cellulase mixtures. Synergistic proteins increase the activity of cellulase without causing significant hydrolysis of cellulose. Synergistic proteins exert their activity by inducing structural modifications in cellulose. Recently, synergistic proteins from various biological sources, including bacteria, fungi, and plants, were identified based on genomic data, and their synergistic activities were investigated. Currently, an up-to-date overview of several aspects of synergistic proteins, such as their functions, action mechanisms and synergistic activity, are important for future industrial application. In this review, we summarize the current state of research on four synergistic proteins: carbohydrate-binding modules, plant expansins, expansin-like proteins, and Auxiliary Activity family 9 (formerly GH61) proteins. This review provides critical information to aid in promoting research on the development of efficient and industrially feasible synergistic proteins.
authors.affiliation: Department of Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea.
authors.forename: In Jung
authors.initials: IJ
authors.lastname: Kim
authors.forename: Hee Jin
authors.initials: HJ
authors.lastname: Lee
authors.forename: In-Geol
authors.initials: IG
authors.lastname: Choi
authors.forename: Kyoung Heon
authors.initials: KH
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D020536:Enzyme Activators / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D006868:Hydrolysis; D010940:Plant Proteins / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D020536:Enzyme Activators; D005656:Fungal Proteins; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C095825:expansin protein, plant; C055213:saccharide-binding proteins; D002482:Cellulose; D002480:Cellulase