title: Acid resistance contributes to the high-pressure carbon dioxide resistance of Escherichia coli K-12.
abstract: Effect of deletion of acid resistant genes of E. coli on the high-pressure carbon dioxide (HPC) resistance was investigated. Genes coding amino acid decarboxylases, such as lysine, arginine, and glutamate decarboxylase, were found to contribute to HPC resistance. Protonophore-treated cells showed hypersensitivity to HPC, confirming that HPC induced cytoplasm acidification and exerted severe damage on cells by intrusion of gaseous carbon dioxide into cytoplasm.
authors.affiliation: Department of Food Bioscience and Biotechnology College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-8510, Japan, furukawa@brs.nihon-u.ac.jp.
authors.forename: Soichi
authors.initials: S
authors.lastname: Furukawa
authors.forename: Junji
authors.initials: J
authors.lastname: Shimazaki
authors.forename: Kazumichi
authors.initials: K
authors.lastname: Kawaharada
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Matsuda
authors.forename: Hiroki
authors.initials: H
authors.lastname: Aoyagi
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Wakabayashi
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Ogihara
authors.forename: Makari
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Morinaga
mesh_terms: D000143:Acids / Q000494:pharmacology*; D002245:Carbon Dioxide / Q000737:chemistry / Q000494:pharmacology*; D048168:Escherichia coli K12 / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D011312:Pressure
chemical_list: D000143:Acids; D029968:Escherichia coli Proteins; D002245:Carbon Dioxide