title: EBP50 regulates the apoptosis of pancreatic cancer cells by decreasing the expression levels of Bcl-2.
abstract: Increasing evidence has demonstrated that ezrin-radixin-moesin (ERM)-binding phosphoprotein 50 (EBP50) is involved in the malignant transformation of numerous human cancers. The present study investigated the involvement of EBP50 overexpression in the tumorigenicity of pancreatic cancer (PC). The results revealed that overexpression of EBP50 suppressed cell growth, promoted cell apoptosis and arrested G1-to-S phase progression in two human PC cell lines. Overexpression of EBP50 also suppressed B-cell lymphoma 2 (Bcl-2) expression. Furthermore, nude mouse tumor xenograft models were established by the subcutaneous injection of cell lines stably transfected with an EBP50-expressing plasmid. Thein vivodata indicated that overexpression of EBP50 inhibited the growth of the PC tumors and induced cell apoptosis. Thus, the present study demonstrated that EBP50 overexpression induces growth inhibition and apoptosis in PC by decreasing Bcl-2 expression. The results suggest that EBP50 may function as a potential tumor suppressorin vivoandin vitro.
authors.affiliation: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
authors.forename: Mengyao
authors.initials: M
authors.lastname: Ji
authors.affiliation: Criminal Science and Technology Studio, Chongqing Banan Police Security Bureau, Chongqing, Chongqing 401320, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Lv
authors.affiliation: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Dong
authors.affiliation: Department of Oncology, The Fifth Hospital of Wuhan, Wuhan 430050, P.R. China.
authors.forename: Xiulan
authors.initials: X
authors.lastname: Peng
keywords: B-cell lymphoma 2; ezrin-radixin-moesin-binding protein 50; overexpression; pancreatic cancer