title: Effect of hyaluronic acid on the thermogelation and biocompatibility of its blends with methyl cellulose.
abstract: Aim of this work was to investigate the influence of hyaluronic acid (HA) molecular weight on the thermogelation and biocompatibility of its blends with methyl cellulose in view of a possible application in drug delivery and/or wound healing. We found out that it was possible to obtain MC/HA blends showing a rheological behavior typical of a viscous solution at 20 °C and of a weak gel at 37 °C only when blending MC with low molecular weight HA. Moreover, the blends containing low molecular weight HA did not affect human foreskin fetal fibroblasts viability, proliferation and migration. On the contrary, the cell incubation with high molecular weight HA resulted in a marked and significant reduction of cell viability, compared to control cells. Finally, the optimized blends, in terms of rheological properties and biocompatibility, proved to be able to control and prolong bovine serum albumin release by a combined mechanism of platform dissolution and drug diffusion.
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Napoli Federico II, Via D. Montesano 49, 80131 Napoli, Italy; Interdisciplinary Research Centre on Biomaterials, CRIB, Università di Napoli Federico II, P.le Tecchio 80, Napoli, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Mayol
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Napoli Federico II, Via D. Montesano 49, 80131 Napoli, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: De Stefano
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Napoli Federico II, Via D. Montesano 49, 80131 Napoli, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: De Falco
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Napoli Federico II, Via D. Montesano 49, 80131 Napoli, Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Carnuccio
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Napoli Federico II, Via D. Montesano 49, 80131 Napoli, Italy; INSERM, U848, IGR, 39 rue C. Desmoulins, 94805 Villejuif, France.
authors.forename: Maria Chiara
authors.initials: MC
authors.lastname: Maiuri
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Napoli Federico II, Via D. Montesano 49, 80131 Napoli, Italy; Interdisciplinary Research Centre on Biomaterials, CRIB, Università di Napoli Federico II, P.le Tecchio 80, Napoli, Italy. Electronic address: gderosa@unina.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: De Rosa
mesh_terms: D001672:Biocompatible Materials; D001831:Body Temperature; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology*; D008422:Materials Testing; D008747:Methylcellulose / Q000737:chemistry* / Q000494:pharmacology*; D008970:Molecular Weight; D012212:Rheology / Q000379:methods; D012710:Serum Albumin, Bovine / Q000493:pharmacokinetics; D012996:Solutions; D014783:Viscosity
chemical_list: D001672:Biocompatible Materials; D012996:Solutions; D012710:Serum Albumin, Bovine; D006820:Hyaluronic Acid; D008747:Methylcellulose
keywords: Drug delivery; Hyaluronic acid; Methylcellulose; Polymeric blend; Thermogelation; Wound healing