title: Serum IL-33 level and IL-33 gene polymorphisms in Behçet's disease.
abstract: Behçet's disease (BD) is a chronic inflammatory disease. Increased productions of cytokines including interleukin (IL)-1β and IL-18 are documented, and IL-1α and β gene polymorphisms are associated with susceptibility to the disease. IL-33 is a recently discovered member of IL-1 cytokine family. The aim of the study was to detect serum IL-33 level and IL-33 gene polymorphisms in a cohort of BD. Unrelated 117 patients with BD and 149 healthy controls (HC) were enrolled. Serum IL-33 levels were analyzed by enzyme-linked immunosorbent assay method. DNA samples were harvested using an appropriate commercial DNA isolation kit. Four single nucleotide polymorphisms of IL-33 gene (rs7044343, rs1157505, rs11792633 and rs1929992) were genotyped using the appropriate commercial primer/probe sets on real-time PCR. Serum IL-33 level was not significantly different in the BD and HC groups (p > 0.05). However, its level was lower in the active BD patients compared to the inactive ones and HC group (p = 0.044 and p = 0.037, respectively). There was no significant difference in terms of the genotypic and allelic distributions of rs1157505 and rs1929992 polymorphisms (p > 0.05 for all). However, the TT variants of rs7044343 and rs11792633 polymorphisms were very rare, and the T allele frequencies of these polymorphisms were lower, in the BD group compared to the HC group (p < 0.0001 for all). The rs7044343 and rs11792633 variants of IL-33 gene are associated with the decreased risk of BD in our cohort. Therefore, it may be concluded that IL-33 acts a protective role on the pathogenesis of BD.
authors.affiliation: Department of Rheumatology, Faculty of Medicine, Firat University, Tip Fakultesi, Romatoloji BD, 23119, Elazig, Turkey, kocassk@yahoo.com.
authors.forename: Suleyman Serdar
authors.initials: SS
authors.lastname: Koca
authors.forename: Murat
authors.initials: M
authors.lastname: Kara
authors.forename: Firat
authors.initials: F
authors.lastname: Deniz
authors.forename: Metin
authors.initials: M
authors.lastname: Ozgen
authors.forename: Caner Feyzi
authors.initials: CF
authors.lastname: Demir
authors.forename: Nevin
authors.initials: N
authors.lastname: Ilhan
authors.forename: Ahmet
authors.initials: A
authors.lastname: Isik
mesh_terms: D000328:Adult; D001528:Behcet Syndrome / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D000067596:Interleukin-33 / Q000097:blood / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: C506536:IL33 protein, human; D000067596:Interleukin-33