title: Genome-wide uncovering of STAT3-mediated miRNA expression profiles in colorectal cancer cell lines.
abstract: Colorectal cancer (CRC) is one of the most common malignancies resulting in high mortality worldwide. Signal transducer and activator of transcription 3 (STAT3) is an oncogenic transcription factor which is frequently activated and aberrantly expressed in CRC. MicroRNAs (miRNAs) are a class of small noncoding RNAs which play important roles in many cancers. However, little is known about the global miRNA profiles mediated by STAT3 in CRC cells. In the present study, we applied RNA interference to inhibit STAT3 expression and profiled the miRNA expression levels regulated by STAT3 in CRC cell lines with deep sequencing. We found that 26 and 21 known miRNAs were significantly overexpressed and downexpressed, respectively, in the STAT3-knockdown CRC cell line SW480 (SW480/STAT3-siRNA) compared to SW480 transfected with scrambled siRNAs (SW480/siRNA-control). The miRNA expression profiling was then validated by quantitative real-time PCR for selected known miRNAs. We further predicted the putative target genes for the dysregulated miRNAs and carried out functional annotation including GO enrichment and KEGG pathway analysis for selected miRNA targets. This study directly depicts STAT3-mediated miRNA profiles in CRC cells, which provides a possible way to discover biomarkers for CRC therapy.
authors.affiliation: School of Life Science and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Jufeng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Engineering, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Luo
authors.affiliation: Experimental Research Center, First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, China.
authors.forename: Huiming
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Life Science and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Deng
authors.affiliation: Biomedical Research Institute, Shenzhen PKU-HKUST Medical Center, No. 1120, Lianhua Road, Futian District, Shenzhen 518036, China.
authors.forename: Ying
authors.identifier: 0000-0001-6195-8202
authors.initials: Y
authors.lastname: Wang
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D058990:Molecular Targeted Therapy; D050796:STAT3 Transcription Factor / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human