title: Clinicopathological and prognostic significance of microRNA-107 in human non small cell lung cancer.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are small, non-coding RNAs which can function as oncogenes or tumor suppressor genes in human cancers. Researchers have found that the expression level of miR-107 was decreased in human non-small cell lung cancer (NSCLC) tissues and cell lines, however, its clinicopathological and prognostic significance in NSCLC has not been investigated.
  
  
  METHODS
  Quantitative real-time PCR (qRT-PCR) was used to analyze the expression of miR-107 in 137 pairs of fresh NSCLC and matched adjacent normal tissue specimens. The chi-square test and Fishers exact tests were used to examine the associations between miR-107 expression and the clinicopathological characters. The overall survival (OS) and progression-free survival (PFS) were analyzed by log-rank test, and survival curves were plotted according to Kaplan-Meier.
  
  
  RESULTS
  The expression level of miR-107 was significantly lower in tumor tissues than that in corresponding noncancerous tissues (0.4676 ± 0.2078 vs. 1.000 ± 0.3953, P<0.001). Low expression of miR-107 was found to significantly correlate with TNM stage (p=0.001), regional lymph node involvement (p=0.04), and tumor differentiation (p=0.003). Kaplan-Meier analysis with the log-rank test indicated that low miR-107 expression had a significant impact on OS (35.2% vs. 69.3%; P=0.008) and PFS (30.0% vs. 56.2%; P=0.029). In a multivariate Cox model, we found that miR-107 expression was an independent poor prognostic factor for both 5-year OS (HR=2.57, 95% CI: 1.88-10.28; P=0.007) and 5-year PFS (HR=3.08, 95% CI: 2.01-8.92; P=0.003).
  
  
  CONCLUSIONS
  The expression of miR-107 was decreased in NSCLC. Low expression of miR-107 was significantly associated with tumor progression and decreased survival in patients with NSCLC, indicating that miR-107 may serve as a novel prognostic marker in NSCLC.
authors.affiliation: Department of Thoracic surgery, Qilu Hospital, Shandong University Jinan, Shandong 250012, China.
authors.forename: Kai-Ze
authors.initials: KZ
authors.lastname: Zhong
authors.affiliation: Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University Jinan, Shandong 250012, China.
authors.forename: Wei-Wen
authors.initials: WW
authors.lastname: Chen
authors.affiliation: Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University Jinan, Shandong 250012, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Hu
authors.affiliation: Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University Jinan, Shandong 250012, China.
authors.forename: An-Li
authors.initials: AL
authors.lastname: Jiang
authors.affiliation: Department of Thoracic surgery, Qilu Hospital, Shandong University Jinan, Shandong 250012, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C549045:MIRN107 microRNA, human; D035683:MicroRNAs
keywords: MicroRNA-107; non-small cell lung cancer; prognosis