title: Benzoic and sorbic acid in soft drink, milk, ketchup sauce and bread by dispersive liquid-liquid microextraction coupled with HPLC.
abstract: Benzoic acid and sorbic acid are widely used for food preservation. These preservatives are generally recognised as safe. The aim of this study was to determine the level of benzoic and sorbic acid in food samples that are usually consumed in Iran. Therefore, 54 samples, including 15 soft drinks, 15 ultra-high-temperature milk, 15 ketchup sauces and 9 bread samples, were analysed by high-performance liquid chromatography with UV detection. Benzoic acid was detected in 50 (92.5%) of the samples ranging from 3.5 to 1520 µg mL⁻¹, while sorbic acid was detected in 29 (50.3%) samples in a range of 0.8 and 2305 µg mL⁻¹. Limits of detection and limits of quantification for benzoate were found to be 0.1 and 0.5 µg mL⁻¹, respectively, and for sorbate 0.08 and 0.3 µg mL⁻¹, respectively. The results showed that benzoic acid and sorbic acid widely occur in food products in Iran.
authors.affiliation: a Drug Applied Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Fardin
authors.initials: F
authors.lastname: Javanmardi
authors.forename: Mahboob
authors.initials: M
authors.lastname: Nemati
authors.forename: Masood
authors.initials: M
authors.lastname: Ansarin
authors.forename: Seyyed Rafie
authors.initials: SR
authors.lastname: Arefhosseini
mesh_terms: D000818:Animals; D019817:Benzoic Acid / Q000032:analysis*; D001628:Beverages / Q000032:analysis* / Q000191:economics; D001939:Bread / Q000032:analysis* / Q000191:economics; D002253:Carbonated Beverages / Q000032:analysis / Q000191:economics; D002851:Chromatography, High Pressure Liquid; D002947:Cities; D003212:Condiments / Q000032:analysis* / Q000191:economics; D005062:European Union; D005513:Food Inspection / Q000379:methods*; D005520:Food Preservatives / Q000032:analysis* / Q000592:standards; D017408:Guidelines as Topic; D007492:Iran; D057230:Limit of Detection; D059627:Liquid Phase Microextraction; D008892:Milk / Q000737:chemistry / Q000191:economics; D061352:Pasteurization; D015203:Reproducibility of Results; D013011:Sorbic Acid / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005520:Food Preservatives; D019817:Benzoic Acid; D013011:Sorbic Acid
keywords: HPLC; Iran; benzoate; dispersive liquid–liquid microextraction; food control; food preservative; sorbate