title: Remediation of petroleum hydrocarbon-contaminated sites by DNA diagnosis-based bioslurping technology.
abstract: The application of effective remediation technologies can benefit from adequate preliminary testing, such as in lab-scale and Pilot-scale systems. Bioremediation technologies have demonstrated tremendous potential with regards to cost, but they cannot be used for all contaminated sites due to limitations in biological activity. The purpose of this study was to develop a DNA diagnostic method that reduces the time to select contaminated sites that are good candidates for bioremediation. We applied an oligonucleotide microarray method to detect and monitor genes that lead to aliphatic and aromatic degradation. Further, the bioremediation of a contaminated site, selected based on the results of the genetic diagnostic method, was achieved successfully by applying bioslurping in field tests. This gene-based diagnostic technique is a powerful tool to evaluate the potential for bioremediation in petroleum hydrocarbon contaminated soil.
authors.affiliation: Environmental Engineering Department, Samsung Engineering Co., Ltd., 500 Samsung GEC, Sangil-Dong, Gangdong-Gu, Seoul 134-728, Republic of Korea.
authors.forename: Seungjin
authors.initials: S
authors.lastname: Kim
authors.affiliation: Center for Environmental Biotechnology, Biodesign Institute at Arizona State University, 1001 South McAllister Avenue, Tempe, AZ 85287-5701, USA.
authors.forename: Rosa
authors.initials: R
authors.lastname: Krajmalnik-Brown
authors.affiliation: Department of Civil and Environmental Engineering, Hanyang University, 222 Wangsimni-Ro, Seongdong-Gu, Seoul 133-791, Republic of Korea.
authors.forename: Jong-Oh
authors.initials: JO
authors.lastname: Kim
authors.affiliation: R&D Center, Samsung Engineering Co., Ltd., 415-10 Woncheon-Dong, Youngtong-Gu, Suwon, Gyeonggi-Do 443-823, Republic of Korea. Electronic address: jin-wook.chung@samsung.com.
authors.forename: Jinwook
authors.initials: J
authors.lastname: Chung
mesh_terms: D001673:Biodegradation, Environmental; D004247:DNA; D052918:Environmental Restoration and Remediation / Q000379:methods*; D006838:Hydrocarbons / Q000032:analysis / Q000378:metabolism*; D007220:Industrial Waste; D010578:Petroleum / Q000032:analysis / Q000378:metabolism*; D012987:Soil; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*
chemical_list: D006838:Hydrocarbons; D007220:Industrial Waste; D010578:Petroleum; D012987:Soil; D012989:Soil Pollutants; D004247:DNA
keywords: BTEX; Bioslurping; Diagnosis technology; Oligonucleotide microarray; TPH