title: IL-17 genetic and immunophenotypic evaluation in chronic graft-versus-host disease.
abstract: Although interleukin-17 (IL-17) is a recently discovered cytokine associated with several autoimmune diseases, its role in the pathogenesis of chronic graft-versus-host disease (cGVHD) was not established yet. The objective of this study was to investigate the association of IL17A and IL17F genes polymorphisms and IL-17A and IL-17F levels with cGVHD. IL-17A expression was also investigated in CD4(+) T cells of patients with systemic cGVHD. For Part I of the study, fifty-eight allo-HSCT recipients and donors were prospectively studied. Blood samples were obtained to determine IL17A and IL17F genes polymorphisms. Cytokines levels in blood and saliva were assessed by ELISA at days +35 and +100 after HSCT. In Part II, for the immunophenotypic evaluation, eight patients with systemic cGVHD were selected and the expression of IL-17A was evaluated. We found association between recipient AA genotype with systemic cGVHD. No association was observed between IL-17A levels and cGVHD. Lower IL-17A levels in the blood were associated with AA genotype. In flow cytometry analysis, decreased expression of IL-17A was observed in patients with cGVHD after stimulation. In conclusion, IL-17A may have an important role in the development of systemic cGVHD.
authors.affiliation: Serviço de Estomatologia, Hospital Municipal Odilon Behrens, Rua Formiga 50, 31110-430 Belo Horizonte, MG, Brazil.
authors.forename: Renata Gonçalves
authors.initials: RG
authors.lastname: Resende
authors.affiliation: Centro Universitário Newton Paiva, Faculdade de Odontologia, Avenida Presidente Carlos Luz 220, 31230-010 Belo Horizonte, MG, Brazil.
authors.forename: Jeane de Fátima
authors.initials: Jde F
authors.lastname: Correia-Silva
authors.affiliation: Departamento de Clínica, Patologia e Cirurgia Odontológicas, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Tarcília Aparecida
authors.initials: TA
authors.lastname: Silva
authors.affiliation: Departamento de Odontologia Restauradora, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Ulisses Eliezer
authors.initials: UE
authors.lastname: Salomão
authors.affiliation: Ciodonto-Universidade FACSETE, Rua Itália Pontelo 50, 35700-170 Sete Lagoas, MG, Brazil.
authors.forename: Luciano
authors.initials: L
authors.lastname: Marques-Silva
authors.affiliation: Laboratório Interdisciplinar de Pesquisa Médica, Faculdade de Medicina, Universidade Federal de Minas Gerais, Avenida Alfredo Balena 190, 30130-10 Belo Horizonte, MG, Brazil.
authors.forename: Érica Leandro Marciano
authors.initials: ÉL
authors.lastname: Vieira
authors.affiliation: Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Walderez Ornelas
authors.initials: WO
authors.lastname: Dutra
authors.affiliation: Departamento de Clínica, Patologia e Cirurgia Odontológicas, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Ricardo Santiago
authors.initials: RS
authors.lastname: Gomez
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005838:Genotype; D006086:Graft vs Host Disease / Q000235:genetics*; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics; D055815:Young Adult
chemical_list: D020381:Interleukin-17