title: Noninvasive detection of sleep/wake changes and cataplexy-like behaviors in orexin/ataxin-3 transgenic narcoleptic mice across the disease onset.
abstract: Sleep and behavioral monitoring of young mice is necessary for understating the progress of symptoms in congenital and acquired diseases associated with sleep and movement disorders. In the current study, we have developed a non-invasive sleep monitoring system that identifies wake and sleep patterns of newborn mice using a simple piezoelectric transducer (PZT). Using this system, we have succeeded in detecting age-dependent occurrences and changes in sleep fragmentation of orexin/ataxin-3 narcoleptic mice (a narcoleptic mouse model with postnatal hypocretin/orexin cell death) across the disease onset. We also detected REM sleep/cataplexy patterns (i.e., immobility with clear heartbeat [IMHB] signals due to the flaccid posture) by the PZT system, and found that sudden onset of REM sleep-like episodes specifically occur in narcoleptic, but not in wild type mice, suggesting that these episodes are likely cataplexy. In contrast, gradual onset of IMHB likely reflects occurrence of REM sleep. In summary, we have shown that the PZT system is useful as a non-invasive sleep and behavior monitoring system to analyze the developmental aspects of sleep and movement disorders in mice models.
authors.affiliation: Sleep and Circadian Neurobiology Laboratory, Stanford University, Palo Alto, CA, USA; Department of Neuropsychiatry, Akita University Graduate School of Medicine, Akita, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Sato
authors.affiliation: Sleep and Circadian Neurobiology Laboratory, Stanford University, Palo Alto, CA, USA; Department of Neuropsychiatry, Akita University Graduate School of Medicine, Akita, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Sagawa
authors.affiliation: Sleep and Circadian Neurobiology Laboratory, Stanford University, Palo Alto, CA, USA.
authors.forename: Nobuhide
authors.initials: N
authors.lastname: Hirai
authors.affiliation: Sleep and Circadian Neurobiology Laboratory, Stanford University, Palo Alto, CA, USA; Department of Cell Physiology, Akita University Graduate School of Medicine, Akita, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sato
authors.affiliation: Sleep and Circadian Neurobiology Laboratory, Stanford University, Palo Alto, CA, USA; Department of Geriatric Medicine, Kanazawa Medical University, Kanazawa, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Okuro
authors.affiliation: Sleep and Circadian Neurobiology Laboratory, Stanford University, Palo Alto, CA, USA.
authors.forename: Samika
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Neuropsychiatry, Akita University Graduate School of Medicine, Akita, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanbayashi
authors.affiliation: Department of Neuropsychiatry, Akita University Graduate School of Medicine, Akita, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Shimizu
authors.affiliation: Sleep and Circadian Neurobiology Laboratory, Stanford University, Palo Alto, CA, USA.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Sakai
authors.affiliation: Sleep and Circadian Neurobiology Laboratory, Stanford University, Palo Alto, CA, USA. Electronic address: nishino@stanford.edu.
authors.forename: Seiji
authors.initials: S
authors.lastname: Nishino
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000067699:Ataxin-3; D002385:Cataplexy / Q000175:diagnosis* / Q000209:etiology*; D004195:Disease Models, Animal; D004569:Electroencephalography; D004576:Electromyography; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009290:Narcolepsy / Q000150:complications* / Q000235:genetics*; D009479:Neuropeptides / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D000068797:Orexins; D012890:Sleep / Q000502:physiology; D012893:Sleep Wake Disorders / Q000175:diagnosis* / Q000209:etiology*; D014157:Transcription Factors / Q000235:genetics; D014159:Transducers; D014851:Wakefulness / Q000502:physiology
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; D009687:Nuclear Proteins; D000068797:Orexins; D014157:Transcription Factors; D000067699:Ataxin-3; C511594:Atxn3 protein, mouse
keywords: Cataplexy; Disease onset; Hypocretin/orexin; Mice; Narcolepsy; Piezoelectric system; REM sleep