title: The crosslinking of polysaccharides with polyamines and dextran-polyallylamine antibacterial hydrogels.
abstract: A facile modular approach to rapidly prepare pH-responsive hydrogels by crosslinking polysaccharides with polyamines is demonstrated. Hydrogels are prepared by first reacting the less reactive polysaccharides with the cross-linker epichlorohydrin and completed by the addition of polyamines. The crosslinking of polysaccharides with polyamines provides a facile method for incorporating functionality into polysaccharide based hydrogels. This process is demonstrated with the polysaccharides dextran, pullulan and carboxymethyl cellulose and with the polyamines polyallylamine and polyethylene imine. The hydrogels were characterized by FTIR and swelling studies, which showed pH-dependent swelling due to the presence of the polyamine. The hydrogels can also be tailored by varying the mass ratio between the polysaccharide and polyamine. Absorption studies of organic analytes showed the polyamine content affecting the uptake of a charged substrate (methylene blue) and no effect on a neutral substrate (6-methyl coumarin). This synthetic method was also used to prepare hydrogels with antibacterial activity against E. coli and S. aureus by utilizing an amphiphilic polyallylamine.
authors.affiliation: Department of Chemistry, Lehman College of the City University of New York, Bronx, NY 10468, United States. Electronic address: naphtali.oconnor@lehman.cuny.edu.
authors.forename: Naphtali A
authors.initials: NA
authors.lastname: O'Connor
authors.affiliation: Department of Chemistry, Lehman College of the City University of New York, Bronx, NY 10468, United States.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Abugharbieh
authors.affiliation: Department of Biology, Lehman College of the City University of New York, Bronx, NY 10468, United States.
authors.forename: Farzana
authors.initials: F
authors.lastname: Yasmeen
authors.affiliation: Department of Chemistry, Lehman College of the City University of New York, Bronx, NY 10468, United States.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Buabeng
authors.affiliation: Department of Chemistry, Lehman College of the City University of New York, Bronx, NY 10468, United States.
authors.forename: Steve
authors.initials: S
authors.lastname: Mathew
authors.affiliation: Department of Chemistry, New York City College of Technology, 300 Jay Street, Brooklyn, NY 11201, United States.
authors.forename: Diana
authors.initials: D
authors.lastname: Samaroo
authors.affiliation: Department of Biology, Lehman College of the City University of New York, Bronx, NY 10468, United States.
authors.forename: Hai-Ping
authors.initials: HP
authors.lastname: Cheng
mesh_terms: D065966:Absorption, Physicochemical; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D003911:Dextrans / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D003432:Cross-Linking Reagents; D003911:Dextrans; D020100:Hydrogels; D011073:Polyamines; C063994:polyallylamine
keywords: Anti-bacterial hydrogel; Polysaccharide polyamine hydrogel; pH-responsive hydrogel