title: Genome size estimates for crustaceans using Feulgen image analysis densitometry of ethanol-preserved tissues.
abstract: Crustaceans are enormously diverse both phylogenetically and ecologically, but they remain substantially underrepresented in the existing genome size database. An expansion of this dataset could be facilitated if it were possible to obtain genome size estimates from ethanol-preserved specimens. In this study, two tests were performed in order to assess the reliability of genome size data generated using preserved material. First, the results of estimates based on flash-frozen versus ethanol-preserved material were compared across 37 species of crustaceans that differ widely in genome size. Second, a comparison was made of specimens from a single species that had been stored in ethanol for 1-14 years. In both cases, the use of gill tissue in Feulgen image analysis densitometry proved to be a very viable approach. This finding is of direct relevance to both new studies of field-collected crustaceans as well as potential studies based on existing collections.
authors.affiliation: Department of Integrative Biology, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: Nicholas W
authors.initials: NW
authors.lastname: Jeffery
authors.forename: T Ryan
authors.initials: TR
authors.lastname: Gregory
mesh_terms: D000818:Animals; D003445:Crustacea / Q000166:cytology / Q000235:genetics*; D015925:Cryopreservation / Q000379:methods*; D003720:Densitometry / Q000379:methods*; D000431:Ethanol / Q000008:administration & dosage*; D059646:Genome Size / Q000235:genetics*; D012394:Rosaniline Dyes / Q000008:administration & dosage*
chemical_list: C007722:Feulgen stain; D012394:Rosaniline Dyes; D000431:Ethanol
keywords: C-value; Crustacea; DNA content; nuclei