title: L-3-n-butylphthalide improves cognitive impairment of APP/PS1 mice by BDNF/TrkB/PI3K/AKT pathway.
abstract: L-3-n-butylphthalide (L-NBP), an extract from seeds of Apium graveolens Linn (Chinese celery), has been shown to have neuroprotective effects on cerebral ischemic, vascular dementia and amyloid-beta (Abeta)-induced animal models by inhibiting oxidative injury, neuronal apoptosis and glial activation, regulating amyloid-beta protein precursor (AbetaPP) processing and reducing Abeta generation. The objective of this study was to investigate the effects of L-3-n-butylphthalide on memory impairment and the expression of brain neurotrophic derived factor (BNDF), kinaseB (TrkB), phosphatidylinositol 3 kinase (PI3K) and Akt in APP/PS1 double transgenic mouse models. APP/PS1 double transgenic mice were administered 30 mg/kg•d L-NBP and 10 mg/kg•d L-NBP for one month. The learning and memory ability were studied using the water maze test. Protein expression and transcript levels of genes in the mice hippocampus were evaluated using western blot and quantitative reverse transcription-polymerase chain reaction (qRT-PCR), respectively. The results demonstrated that both 30 mg/kg•d L-NBP and 10 mg/kg•d L-NBP doses of L-NBP significantly increased memory capability and the expression of hippocampal BDNF/TrkB/PI3K/AKT in mice The results suggested that L-NBP treatment may reverse memory impairment in APP/PS1 transgenic mice, and BDNF/TrkB/PI3K/AKT, may be involved in this process.
authors.affiliation: Department of Pharmacology, First Hospital of China Medical University Shenyang 110001, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xiang
authors.affiliation: Department of Pharmacology, Xiangyang No. 1 People's Hospital, Hubei University of Medicine Xiangyang 441000, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Pan
authors.affiliation: Department of Pharmacology, First Hospital of China Medical University Shenyang 110001, China.
authors.forename: Fujun
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Pharmacology, First Hospital of China Medical University Shenyang 110001, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Department of Pharmacology, First Hospital of China Medical University Shenyang 110001, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Pharmacology, First Hospital of China Medical University Shenyang 110001, China.
authors.forename: Shutao
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, First Hospital of China Medical University Shenyang 110001, China.
authors.forename: Wanyu
authors.initials: W
authors.lastname: Feng
keywords: APP/PS1; BDNF/TrkB/PI3K/AKT; L-3-n-butylphthalide; mice