title: Expression profiles of 12 late embryogenesis abundant protein genes from Tamarix hispida in response to abiotic stress.
abstract: Twelve embryogenesis abundant protein (LEA) genes (named ThLEA-1 to -12) were cloned from Tamarix hispida. The expression profiles of these genes in response to NaCl, PEG, and abscisic acid (ABA) in roots, stems, and leaves of T. hispida were assessed using real-time reverse transcriptase-polymerase chain reaction (RT-PCR). These ThLEAs all showed tissue-specific expression patterns in roots, stems, and leaves under normal growth conditions. However, they shared a high similar expression patterns in the roots, stems, and leaves when exposed to NaCl and PEG stress. Furthermore, ThLEA-1, -2, -3, -4, and -11 were induced by NaCl and PEG, but ThLEA-5, -6, -8, -10, and -12 were downregulated by salt and drought stresses. Under ABA treatment, some ThLEA genes, such as ThLEA-1, -2, and -3, were only slightly differentially expressed in roots, stems, and leaves, indicating that they may be involved in the ABA-independent signaling pathway. These findings provide a basis for the elucidation of the function of LEA genes in future work.
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China ; Key Laboratory of Forest Genetics & Biotechnology, Nanjing Forestry University, Ministry of Education, Nanjing 210037, China.
authors.forename: Caiqiu
authors.initials: C
authors.lastname: Gao
authors.affiliation: Northeast Forestry University Library, 26 Hexing Road, Harbin 150040, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
authors.forename: Kaimin
authors.initials: K
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Wang
mesh_terms: D018506:Gene Expression Regulation, Plant; D009928:Organ Specificity; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D013312:Stress, Physiological; D032363:Tamaricaceae / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; C066246:late embryogenesis abundant protein, plant