title: Weak self-interactions of globular proteins studied by small-angle X-ray scattering and structure-based modeling.
abstract: We investigate protein-protein interactions in solution by small-angle X-ray scattering (SAXS) and theoretical modeling. The structure factor for solutions of bovine pancreatic trypsin inhibitor (BPTI), myoglobin (Mb), and intestinal fatty acid-binding protein (IFABP) is determined from SAXS measurements at multiple concentrations, from Monte Carlo simulations with a coarse-grained structure-based interaction model, and from analytic approximate solutions of two idealized colloidal interaction models without adjustable parameters. By combining these approaches, we find that the structure factor is essentially determined by hard-core and screened electrostatic interactions. Other soft short-ranged interactions (van der Waals and solvation-related) are either individually insignificant or tend to cancel out. The structure factor is also not significantly affected by charge fluctuations. For Mb and IFABP, with a small net charge and relatively symmetric charge distribution, the structure factor is well described by a hard-sphere model. For BPTI, with a larger net charge, screened electrostatic repulsion is also important, but the asymmetry of the charge distribution reduces the repulsion from that predicted by a charged hard-sphere model with the same net charge. Such charge asymmetry may also amplify the effect of shape asymmetry on the protein-protein potential of mean force.
authors.affiliation: Department of Biophysical Chemistry, Lund University , P.O. Box 124, SE-22100 Lund, Sweden.
authors.forename: Shuji
authors.initials: S
authors.lastname: Kaieda
authors.forename: Mikael
authors.initials: M
authors.lastname: Lund
authors.forename: Tomás S
authors.initials: TS
authors.lastname: Plivelic
authors.forename: Bertil
authors.initials: B
authors.lastname: Halle
mesh_terms: D000818:Animals; D007611:Aprotinin / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D050556:Fatty Acid-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D009010:Monte Carlo Method; D009211:Myoglobin / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D025941:Protein Interaction Mapping; D053838:Scattering, Small Angle; D055672:Static Electricity; D014961:X-Ray Diffraction
chemical_list: D050556:Fatty Acid-Binding Proteins; D009211:Myoglobin; D007611:Aprotinin