title: Detection of an undescribed Rickettsia sp. in Ixodes boliviensis from Costa Rica.
abstract: Ixodes boliviensis is a tick of carnivores that is common on domestic dogs. The only Rickettsia that has been detected previously in this species is 'Candidatus Rickettsia andeanae'. We report the detection of an undescribed Rickettsia sp., named strain IbR/CRC, in I. boliviensis collected from dogs in Costa Rica. Analyses of gltA, ompA, and htrA partial sequences place Rickettsia sp. strain IbR/CRC in the group of R. monacensis, also close to an endosymbiont of Ixodes scapularis and other undescribed rickettsiae. It was not possible to isolate Rickettsia sp. strain IbR/CRC in Vero E6 or C6/36 cell lines. Isolation and further characterization of Rickettsia sp. strain IbR/CRC and the other undescribed rickettsiae are required to determine their taxonomic status and pathogenic potential.
authors.affiliation: Centro de Investigación en Enfermedades Tropicales, Universidad de Costa Rica, San José, Costa Rica; Sección de Entomología Médica, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica. Electronic address: adriana.troyo@ucr.ac.cr.
authors.forename: Adriana
authors.initials: A
authors.lastname: Troyo
authors.affiliation: Centro de Investigación en Enfermedades Tropicales, Universidad de Costa Rica, San José, Costa Rica; Sección de Virología, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.
authors.forename: Andrés
authors.initials: A
authors.lastname: Moreira-Soto
authors.affiliation: Centro de Investigación en Enfermedades Tropicales, Universidad de Costa Rica, San José, Costa Rica.
authors.forename: Marco
authors.initials: M
authors.lastname: Carranza
authors.affiliation: Centro de Investigación en Enfermedades Tropicales, Universidad de Costa Rica, San José, Costa Rica; Sección de Entomología Médica, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.
authors.forename: Olger
authors.initials: O
authors.lastname: Calderón-Arguedas
authors.affiliation: Centro de Investigación en Enfermedades Tropicales, Universidad de Costa Rica, San José, Costa Rica; Sección de Virología, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.
authors.forename: Laya
authors.initials: L
authors.lastname: Hun
authors.affiliation: Centro de Investigación en Enfermedades Tropicales, Universidad de Costa Rica, San José, Costa Rica; Sección de Virología, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.
authors.forename: Lizeth
authors.initials: L
authors.lastname: Taylor
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D003364:Costa Rica / Q000453:epidemiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology / Q000382:microbiology*; D004285:Dogs; D018884:Ixodes / Q000382:microbiology*; D008969:Molecular Sequence Data; D012281:Rickettsia / Q000235:genetics / Q000302:isolation & purification*; D012282:Rickettsia Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013559:Symbiosis; D014709:Vero Cells
chemical_list: D004269:DNA, Bacterial
keywords: Costa Rica; Endosymbiont; Ixodes boliviensis; Rickettsia