title: MiR-150-5p suppresses colorectal cancer cell migration and invasion through targeting MUC4.
abstract: Growing evidence suggests that miR-150-5p has an important role in regulating genesis of various types of cancer. However, the roles and the underlying mechanisms of miR-150-5p in development of colorectal cancer (CRC) remain largely unknown. Transwell chambers were used to analyze effects on cell migration and invasion by miR-150-5p. Quantitative real-time PCR (qRT-PCR), Western blotting and dual-luciferase 3' UTR reporter assay were carried out to identify the target genes of miR-150-5p. In our research, miR-150-5p suppressed CRC cell migration and invasion, and MUC4 was identified as a direct target gene. Its effects were partly blocked by re-expression of MUC4. In conclusiomn, miR-150-5p may suppress CRC metastasis through directly targeting MUC4, highlighting its potential as a novel agent for the treatment of CRC metastasis.
authors.affiliation: Department of Clinical Laboratory, The Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, China E-mail : jinhua_dainb@126.com.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.forename: Bu-Rong
authors.initials: BR
authors.lastname: Zhang
authors.forename: Hong-Sheng
authors.initials: HS
authors.lastname: Yu
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Jin
authors.forename: Jin-Hua
authors.initials: JH
authors.lastname: Dai
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D015153:Blotting, Western; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D055264:Mucin-4 / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D020413:3' Untranslated Regions; D008156:Luciferases; D035683:MicroRNAs; D055264:Mucin-4; D012333:RNA, Messenger; C533097:MIR150, human; C524658:MUC4 protein, human