title: Silver nanoparticles/ibuprofen-loaded poly(L-lactide) fibrous membrane: anti-infection and anti-adhesion effects.
abstract: Infection caused by bacteria is one of the crucial risk factors for tendon adhesion formation. Silver nanoparticles (AgNP)-loaded physical barriers were reported to be effective in anti-infection and anti-adhesion. However, high silver load may lead to kidney and liver damages. This study was designed for Ibuprofen (IBU)-loaded poly(L-lactide) (PLLA) electrospun fibrous membranes containing a low dosage of Ag to evaluate its potential in maintaining suitable anti-infection and good anti-adhesion effects. The in vitro drug release study showed a sustained release of Ag ions and IBU from the membrane. Inferior adherence and proliferation of fibroblasts were found on the Ag4%-IBU4%-loaded PLLA electrospun fibrous membranes in comparison with pure PLLA and 4% Ag-loaded PLLA membranes. In the antibacterial test, all Ag-loaded PLLA electrospun fibrous membranes prevented the adhesion of Staphylococcus aureus and Staphylococcus epidermidis. Taken together, these results demonstrate that Ibuprofen is effective in enhancing the anti-adhesion and anti-proliferation effects of 4% Ag-loaded PLLA fibrous membrane. The medical potential of infection reduction and adhesion prevention of Ag4%-IBU4%-loaded PLLA electrospun fibrous membrane deserves to be further studied.
authors.affiliation: Department of Orthopaedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, 600 Yishan Road, Shanghai 200233, China. hikev00@163.com.
authors.forename: Shuai
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Orthopaedic, Yuhuangding Hospital of Yantai, 20 Yuhuangding East Road, Yantai 264000, China. xlnedmd@126.com.
authors.forename: Guangda
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics and Traumatology, Faculty of Medicine, the Chinese University of Hong Kong, Hong Kong, China. bxnmephd@126.com.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Wu
authors.affiliation: Orthopedic Institute, Soochow University, 708 Renmin Road, Suzhou 215006, China. crpi688@126.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Orthopaedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, 600 Yishan Road, Shanghai 200233, China. liushensjtu@126.com.
authors.forename: Shen
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics and Traumatology, Faculty of Medicine, the Chinese University of Hong Kong, Hong Kong, China. jgl100n@126.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Orthopedic Institute, Soochow University, 708 Renmin Road, Suzhou 215006, China. wgcui80@hotmail.com.
authors.forename: Wenguo
authors.initials: W
authors.lastname: Cui
authors.affiliation: Department of Orthopaedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, 600 Yishan Road, Shanghai 200233, China. fancunyi888@hotmail.com.
authors.forename: Cunyi
authors.initials: C
authors.lastname: Fan
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry*; D007052:Ibuprofen / Q000737:chemistry* / Q000494:pharmacology; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D041681:NIH 3T3 Cells; D011091:Polyesters / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects
chemical_list: D004337:Drug Carriers; D011091:Polyesters; D012834:Silver; C033616:poly(lactide); D007052:Ibuprofen