title: Heat shock protein 83 (Hsp83) facilitates methoprene-tolerant (Met) nuclear import to modulate juvenile hormone signaling.
abstract: Juvenile hormone (JH) receptors, methoprene-tolerant (Met) and Germ-cell expressed (Gce), transduce JH signals to induce Kr-h1 expression in Drosophila. Dual luciferase assay identified a 120-bp JH response region (JHRR) in the Kr-h1α promoter. Both in vitro and in vivo experiments revealed that Met and Gce transduce JH signals to induce Kr-h1 expression through the JHRR. DNA affinity purification identified chaperone protein Hsp83 as one of the proteins bound to the JHRR in the presence of JH. Interestingly, Hsp83 physically interacts with PAS-B and basic helix-loop-helix domains of Met, and JH induces Met-Hsp83 interaction. As determined by immunohistochemistry, Met is mainly distributed in the cytoplasm of fat body cells of the larval when the JH titer is low and JH induces Met nuclear import. Hsp83 was accumulated in the cytoplasm area adjunct to the nucleus in the presence of JH and Met/Gce. Loss-of-function of Hsp83 attenuated JH binding and JH-induced nuclear import of Met, resulting in a decrease in the JHRR-driven reporter activity leading to reduction of Kr-h1 expression. These data show that Hsp83 facilitates the JH-induced nuclear import of Met that induces Kr-h1 expression through the JHRR.
authors.affiliation: From the Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China, the College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
authors.forename: Qianyu
authors.initials: Q
authors.lastname: He
authors.affiliation: From the Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Di
authors.initials: D
authors.lastname: Wen
authors.affiliation: From the Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Qiangqiang
authors.initials: Q
authors.lastname: Jia
authors.affiliation: From the Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Chunlai
authors.initials: C
authors.lastname: Cui
authors.affiliation: the Department of Entomology, University of Maryland, College Park, Maryland 20742, and.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: the Department of Entomology, College of Agriculture, University of Kentucky, Lexington, Kentucky 40546.
authors.forename: Subba R
authors.initials: SR
authors.lastname: Palli
authors.affiliation: From the Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China, lisheng01@sibs.ac.cn.
authors.forename: Sheng
authors.initials: S
authors.lastname: Li
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D004330:Drosophila / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D007605:Juvenile Hormones / Q000378:metabolism*; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008726:Methoprene / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D015398:Signal Transduction
chemical_list: D029721:Drosophila Proteins; D006360:Heat-Shock Proteins; C089272:Hsp83 protein, Drosophila; D007605:Juvenile Hormones; C497242:Kr-h1 protein, Drosophila; D051741:Kruppel-Like Transcription Factors; D008726:Methoprene
keywords: Basic Helix-loop-helix Transcription Factor (bHLH); Cytoplasm-Nucleus Shuttling; Drosophila; Heat Shock Protein 90 (Hsp90); Hormone Receptor; Insect; Insect Hormones; Molecular Chaperone;; bHLH Transcription Factor