title: [Effect of ambroxol on biofilm of Haemophilus influenzae and bactericidal action].
abstract: OBJECTIVE
  To establish a biofilm model of Haemophilus influenzae and observe the effect of ambroxol on biofilm of Haemophilus influenzae and bactericidal action.
  
  
  METHODS
  Thirty strains of Haemophilus influenzae were isolated from adenoids of children with adenoidal hypertrophy. Two strains which could build stronger biofilms was selected in a 96-well plate. The effect of ambroxol on biofilms were determined by crystal violet, and the structure of biofilms were observed by scanning electron microscope (SEM). The numbers of viable bacterial in biofilm after ambroxol treatmented determined by plate culture count.
  
  
  RESULTS
  Through crystal violet assay, significant difference (P < 0.01) between the two group after treatment was found when ambroxol concentration reached at 0.25 mg/ml and 0.49 mg/ml. The biofilms was destroyed by SEM. Ambroxol had the positive effect on bacterial killing by plate culture count,and the effect was in a dose dependent.
  
  
  CONCLUSIONS
  Ambroxol could destroy the biofilm of Haemophilus influenzae, and had bactericidal function in vitro.
authors.forename: Xue
authors.initials: X
authors.lastname: Gao
authors.forename: Yutuo
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yantao
authors.initials: Y
authors.lastname: Lin
authors.forename: Haifeng
authors.initials: H
authors.lastname: Li
authors.forename: Yunchao
authors.initials: Y
authors.lastname: Xin
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zhang
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Xu
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Shang
mesh_terms: D000551:Ambroxol / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002648:Child; D002675:Child, Preschool; D006193:Haemophilus influenzae / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests
chemical_list: D000551:Ambroxol