title: A highly responsive and selective fluorescent probe for imaging physiological hydrogen sulfide.
abstract: The discovery of hydrogen sulfide (H2S) as a novel gasotransmitter for cell signaling and other pathophysiological processes has spurred tremendous interest in developing analytical methods for its detection in biological systems. Herein, we report the development of a highly responsive and selective genetically encoded H2S probe, hsGFP, for the detection of H2S both in vitro and in living mammalian cells. hsGFP bestows a combination of favorable properties, including large fluorescence responses, high efficiency in folding and chromophore formation, and excellent sensitivity and selectivity toward H2S. As a genetically encoded probe, hsGFP can be readily and precisely localized to subcellular domains such as mitochondria, cell nuclei, and ion channels. hsGFP was further utilized to image H2S enzymatically produced from l-cysteine in human embryonic kidney (HEK) 293T cells.
authors.affiliation: Department of Chemistry, University of California , 501 Big Springs Road, Riverside, California 92521, United States.
authors.forename: Zhi-jie
authors.initials: ZJ
authors.lastname: Chen
authors.forename: Hui-wang
authors.initials: HW
authors.lastname: Ai
mesh_terms: D000595:Amino Acid Sequence; D015374:Biosensing Techniques / Q000379:methods; D003545:Cysteine; D005456:Fluorescent Dyes / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis*; D057054:Molecular Imaging / Q000379:methods*; D008969:Molecular Sequence Data; D015202:Protein Engineering
chemical_list: D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D003545:Cysteine; D006862:Hydrogen Sulfide