title: CTAB turbidimetric method for assaying hyaluronic acid in complex environments and under cross-linked form.
abstract: The cetyltrimethylammonium bromide turbidimetric method (CTM) has been developed to quantify the hyaluronic acid (HA) in complex media to overcome the lack of selectivity and specificity of the standard carbazole method. The objective of this work is to assess the potential application of CTM to determine HA concentration. Factors such as duration of incubation, linearity range, HA size and form (natural linear HA or cross linked HA), pH and ionic environment impact were investigated. The incubation time was set to 10 min and the calibration curve was linear up to 0.6 g L(-1). The quantitative method was relevant whatever the HA size and form, and also for a wide range of conditions. The robustness of the CTM added to its high specificity and simplicity demonstrated that the CTM is a valuable method that would be an interesting substitute to the carbazole assay for HA quantification.
authors.affiliation: Laboratoire Réaction et Génie des Procédés, C.N.R.S.-U.M.R.-7274, plateforme SVS, 13 rue du bois de la Champelle, F-54500 Vandœuvre-lès-Nancy, France; Université de Lorraine, U.M.R.-7274, 2 avenue de la forêt de Haye, F-54505 Vandœuvre-lès-Nancy, France.
authors.forename: Nadia
authors.initials: N
authors.lastname: Oueslati
authors.affiliation: Laboratoire Réaction et Génie des Procédés, C.N.R.S.-U.M.R.-7274, plateforme SVS, 13 rue du bois de la Champelle, F-54500 Vandœuvre-lès-Nancy, France; Université de Lorraine, U.M.R.-7274, 2 avenue de la forêt de Haye, F-54505 Vandœuvre-lès-Nancy, France.
authors.forename: Pierrick
authors.initials: P
authors.lastname: Leblanc
authors.affiliation: Laboratoire Réaction et Génie des Procédés, C.N.R.S.-U.M.R.-7274, plateforme SVS, 13 rue du bois de la Champelle, F-54500 Vandœuvre-lès-Nancy, France; Université de Lorraine, U.M.R.-7274, 2 avenue de la forêt de Haye, F-54505 Vandœuvre-lès-Nancy, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Harscoat-Schiavo
authors.affiliation: Laboratoire Réaction et Génie des Procédés, C.N.R.S.-U.M.R.-7274, plateforme SVS, 13 rue du bois de la Champelle, F-54500 Vandœuvre-lès-Nancy, France; Université de Lorraine, U.M.R.-7274, 2 avenue de la forêt de Haye, F-54505 Vandœuvre-lès-Nancy, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Rondags
authors.affiliation: Teoxane Geneva, 105 rue de Lyon Les Charmilles, CH1203 Geneva, Switzerland.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Meunier
authors.affiliation: Laboratoire Réaction et Génie des Procédés, C.N.R.S.-U.M.R.-7274, plateforme SVS, 13 rue du bois de la Champelle, F-54500 Vandœuvre-lès-Nancy, France; Université de Lorraine, U.M.R.-7274, 2 avenue de la forêt de Haye, F-54505 Vandœuvre-lès-Nancy, France. Electronic address: romain.kapel@univ-lorraine.fr.
authors.forename: Romain
authors.initials: R
authors.lastname: Kapel
authors.affiliation: Laboratoire Réaction et Génie des Procédés, C.N.R.S.-U.M.R.-7274, plateforme SVS, 13 rue du bois de la Champelle, F-54500 Vandœuvre-lès-Nancy, France; Université de Lorraine, U.M.R.-7274, 2 avenue de la forêt de Haye, F-54505 Vandœuvre-lès-Nancy, France.
authors.forename: Ivan
authors.initials: I
authors.lastname: Marc
mesh_terms: D002072:Butylene Glycols / Q000737:chemistry; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D006820:Hyaluronic Acid / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D002072:Butylene Glycols; D002593:Cetrimonium Compounds; D003432:Cross-Linking Reagents; D006820:Hyaluronic Acid; C014376:1,4-bis(2,3-epoxypropoxy)butane; D000077286:Cetrimonium
keywords: Analytical method; Carbazole; Cetyltrimethylammonium bromide; Hyaluronic acid