title: The effects of serotonin1A receptor on female mice body weight and food intake are associated with the differential expression of hypothalamic neuropeptides and the GABAA receptor.
abstract: Both common eating disorders anorexia nervosa and bulimia nervosa are characteristically diseases of women. To characterize the role of the 5-HT1A receptor (5-HT1A-R) in these eating disorders in females, we investigated the effect of saline or 8-hydroxy-2-(di-n-propylamino) tetralin (8-OH-DPAT) treatment on feeding behavior and body weight in adult WT female mice and in adult 5-HT1A-R knockout (KO) female mice. Our results showed that KO female mice have lower food intake and body weight than WT female mice. Administration of 8-OH-DPAT decreased food intake but not body weight in WT female mice. Furthermore, qRT-PCR was employed to analyze the expression levels of neuropeptides, γ-aminobutyric acid A receptor subunit β (GABAA β subunits) and glutamic acid decarboxylase in the hypothalamic area. The results showed the difference in food intake between WT and KO mice was accompanied by differential expression of POMC, CART and GABAA β2, and the difference in body weight between WT and KO mice was associated with significantly different expression levels of CART and GABAA β2. As such, our data provide new insight into the role of 5-HT1A-R in both feeding behavior and the associated expression of neuropeptides and the GABAA receptor.
authors.affiliation: Department of Biology, College of Staten Island, City University of New York, Staten Island, NY 10314, USA.
authors.forename: Isma
authors.initials: I
authors.lastname: Butt
authors.affiliation: Department of Biology, College of Staten Island, City University of New York, Staten Island, NY 10314, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Hong
authors.affiliation: Department of Biology, College of Staten Island, City University of New York, Staten Island, NY 10314, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Di
authors.affiliation: Department of Chemistry, College of Staten Island, City University of New York, Staten Island, NY 10314, USA.
authors.forename: Sonia
authors.initials: S
authors.lastname: Aracena
authors.affiliation: Institute for Macromolecular Assemblies, City University of New York, Staten Island, NY 10314, USA; Department of Chemistry, College of Staten Island, City University of New York, Staten Island, NY 10314, USA.
authors.forename: Probal
authors.initials: P
authors.lastname: Banerjee
authors.affiliation: Department of Biology, College of Staten Island, City University of New York, Staten Island, NY 10314, USA; Institute for Macromolecular Assemblies, City University of New York, Staten Island, NY 10314, USA. Electronic address: ChangHui.Shen@csi.cuny.edu.
authors.forename: Chang-Hui
authors.initials: CH
authors.lastname: Shen
mesh_terms: D017371:8-Hydroxy-2-(di-n-propylamino)tetralin / Q000494:pharmacology; D000818:Animals; D000856:Anorexia Nervosa / Q000378:metabolism*; D001835:Body Weight / Q000502:physiology; D052018:Bulimia Nervosa / Q000378:metabolism*; D005247:Feeding Behavior / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D007031:Hypothalamus / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009479:Neuropeptides / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D044282:Receptor, Serotonin, 5-HT1A / Q000235:genetics / Q000378:metabolism*; D011963:Receptors, GABA-A / Q000378:metabolism; D017366:Serotonin Receptor Agonists / Q000494:pharmacology
chemical_list: D009479:Neuropeptides; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D017366:Serotonin Receptor Agonists; D044282:Receptor, Serotonin, 5-HT1A; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin
keywords: Body weight; Food intake; GABA(A) β subunits; Gene expression; Neuropeptides; Serotonin; qRT-PCR