title: Desipramine and citalopram attenuate pretest swim-induced increases in prodynorphin immunoreactivity in the dorsal bed nucleus of the stria terminalis and the lateral division of the central nucleus of the amygdala in the forced swimming test.
abstract: Dynorphin in the nucleus accumbens shell plays an important role in antidepressant-like effect in the forced swimming test (FST), but it is unclear whether desipramine and citalopram treatments alter prodynorphin levels in other brain areas. To explore this possibility, we injected mice with desipramine and citalopram 0.5, 19, and 23 h after a 15-min pretest swim and observed changes in prodynorphin expression before the test swim, which was conducted 24 h after the pretest swim. The pretest swim increased prodynorphin immunoreactivity in the dorsal bed nucleus of the stria terminalis (dBNST) and lateral division of the central nucleus of the amygdala (CeL). This increase in prodynorphin immunoreactivity in the dBNST and CeL was blocked by desipramine and citalopram treatments. Similar changes in prodynorphin mRNA levels were observed in the dBNST and CeL, but these changes did not reach significance. To understand the underlying mechanism, we assessed changes in phosphorylated CREB at Ser(133) (pCREB) immunoreactivity in the dBNST and central nucleus of the amygdala (CeA). Treatment with citalopram but not desipramine after the pretest swim significantly increased pCREB immunoreactivity only in the dBNST. These results suggest that regulation of prodynorphin in the dBNST and CeL before the test swim may be involved in the antidepressant-like effect of desipramine and citalopram in the FST and suggest that changes in pCREB immunoreactivity in these areas may not play an important role in the regulation of prodynorphin in the dBNST and CeA.
authors.affiliation: Department of Pharmacology, Korea University College of Medicine, Anam-Dong, Sungbuk-Gu, Seoul, Republic of Korea.
authors.forename: Sung
authors.initials: S
authors.lastname: Chung
authors.affiliation: Department of Pharmacology, Korea University College of Medicine, Anam-Dong, Sungbuk-Gu, Seoul, Republic of Korea.
authors.forename: Hee Jeong
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Pharmacology, Korea University College of Medicine, Anam-Dong, Sungbuk-Gu, Seoul, Republic of Korea.
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Pharmacology, Korea University College of Medicine, Anam-Dong, Sungbuk-Gu, Seoul, Republic of Korea.
authors.forename: Sun Hye
authors.initials: SH
authors.lastname: Choi
authors.affiliation: Department of Pharmacology, Korea University College of Medicine, Anam-Dong, Sungbuk-Gu, Seoul, Republic of Korea.
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Department of Pharmacology, Korea University College of Medicine, Anam-Dong, Sungbuk-Gu, Seoul, Republic of Korea.
authors.forename: Yun Ha
authors.initials: YH
authors.lastname: Cho
authors.affiliation: Department of Pharmacology, Korea University College of Medicine, Anam-Dong, Sungbuk-Gu, Seoul, Republic of Korea.
authors.forename: Dong-Hoon
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Department of Pharmacology, Korea University College of Medicine, Anam-Dong, Sungbuk-Gu, Seoul, Republic of Korea. Electronic address: kyungho@korea.ac.kr.
authors.forename: Kyung Ho
authors.initials: KH
authors.lastname: Shin
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D066274:Central Amygdaloid Nucleus / Q000187:drug effects*; D015283:Citalopram / Q000494:pharmacology*; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D003891:Desipramine / Q000494:pharmacology*; D004745:Enkephalins / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010766:Phosphorylation; D011498:Protein Precursors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012686:Septal Nuclei / Q000187:drug effects*; D013315:Stress, Psychological / Q000378:metabolism*; D013550:Swimming
chemical_list: D000928:Antidepressive Agents; D017362:Cyclic AMP Response Element-Binding Protein; D004745:Enkephalins; D011498:Protein Precursors; D012333:RNA, Messenger; D015283:Citalopram; C033660:preproenkephalin; D003891:Desipramine
keywords: Amygdala; Antidepressive agents; Bed nucleus of stria terminalis; FST; Prodynorphin