title: Regulation of fatty acid biosynthesis by the global regulator CcpA and the local regulator FabT in Streptococcus mutans.
abstract: SMU.1745c, encoding a putative transcriptional regulator of the MarR family, maps to a location proximal to the fab gene cluster in Streptococcus mutans. Deletion of the SMU.1745c (fabTS m ) coding region resulted in a membrane fatty acid composition comprised of longer-chained, unsaturated fatty acids (UFA), compared with the parent strain. Previous reports have indicated a role for FabT in regulation of genes in the fab gene cluster in other organisms, through binding to a palindromic DNA sequence. Consensus FabT motif sequences were identified in S. mutans in the intergenic regions preceding fabM, fabTSm and fabK in the fab gene cluster. Chloramphenicol acetyltransferase (cat) reporter fusions, using the fabM promoter, revealed elevated transcription in a ∆fabTS m background. Transcription of fabTS m was dramatically elevated in cells grown at pH values of 5 and 7 in the ∆ fabTS m background. Transcription of fabTS m was also elevated in a strain carrying a deletion for the carbon catabolite repressor CcpA. Purified FabTS m and CcpA bound to the promoter regions of fabTS m and fabM. Hence, the data indicate that FabTS m acts as a repressor of fabM and fabTS m itself and the global regulator CcpA acts as a repressor for fabTS m .
authors.affiliation: Center for Oral Biology, University of Rochester, Rochester, NY, USA.
authors.forename: R C
authors.initials: RC
authors.lastname: Faustoferri
authors.forename: C J
authors.initials: CJ
authors.lastname: Hubbard
authors.forename: B
authors.initials: B
authors.lastname: Santiago
authors.forename: A A
authors.initials: AA
authors.lastname: Buckley
authors.forename: T B
authors.initials: TB
authors.lastname: Seifert
authors.forename: R G
authors.initials: RG
authors.lastname: Quivey
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D005227:Fatty Acids / Q000096:biosynthesis*; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D011401:Promoter Regions, Genetic; D013295:Streptococcus mutans / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D005227:Fatty Acids
keywords: Streptococcus mutans; acid stress response; fatty acid synthesis