title: Increased chromogranin A and neuron-specific enolase in rats with chronic nonbacterial prostatitis induced by 17-beta estradiol combined with castration.
abstract: Although chronic nonbacterial prostatitis (CNBP) is a common diagnosis in middle-aged men, the etiology of this disease remains poorly understood. Neuroendocrine cells play an important role in the neuroendocrine regulation of the prostate, and chromogranin A (CgA) and neuron-specific enolase (NSE) are regarded as classic markers of neuroendocrine cells. This study aimed to determine CgA and NSE levels in a CNBP rat model to evaluate the role of neuroendocrine cells in the pathogenesis of CNBP. For developing a CNBP rat model, we examined the ability of 17-beta estradiol and surgical castration alone or in combination to induce CNBP. Histologic inflammation of the prostate was assessed in CNBP-induced rats by hematoxylin-eosin staining, whereas CgA and NSE protein levels were assessed by immunohistochemistry, Western blot analysis, and enzyme-linked immunosorbent assays. Our results showed that 17-beta estradiol combined with castration successfully induced CNBP and that CgA and NSE levels were increased in the prostate of CNBP rats as compared to those without CNBP. These findings indicate that the neuroendocrine regulation mediated by neuroendocrine cells may be involved in the pathogenesis of CNBP.
authors.affiliation: Department of Urology and Anhui Geriatric Institute, The First Affiliated Hospital of Anhui Medical University Hefei 230022, Anhui, China.
authors.forename: Song
authors.initials: S
authors.lastname: Fan
authors.affiliation: Department of Urology and Anhui Geriatric Institute, The First Affiliated Hospital of Anhui Medical University Hefei 230022, Anhui, China.
authors.forename: Zong-Yao
authors.initials: ZY
authors.lastname: Hao
authors.affiliation: Department of Urology and Anhui Geriatric Institute, The First Affiliated Hospital of Anhui Medical University Hefei 230022, Anhui, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Urology and Anhui Geriatric Institute, The First Affiliated Hospital of Anhui Medical University Hefei 230022, Anhui, China.
authors.forename: Xian-Guo
authors.initials: XG
authors.lastname: Chen
authors.affiliation: Department of Urology and Anhui Geriatric Institute, The First Affiliated Hospital of Anhui Medical University Hefei 230022, Anhui, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Urology and Anhui Geriatric Institute, The First Affiliated Hospital of Anhui Medical University Hefei 230022, Anhui, China.
authors.forename: Yi-Fei
authors.initials: YF
authors.lastname: Zang
authors.affiliation: Department of Urology and Anhui Geriatric Institute, The First Affiliated Hospital of Anhui Medical University Hefei 230022, Anhui, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Tai
authors.affiliation: Department of Urology and Anhui Geriatric Institute, The First Affiliated Hospital of Anhui Medical University Hefei 230022, Anhui, China.
authors.forename: Chao-Zhao
authors.initials: CZ
authors.lastname: Liang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D053379:Chromogranin A / Q000032:analysis / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000633:toxicity; D007150:Immunohistochemistry; D008297:Male; D055099:Neuroendocrine Cells / Q000378:metabolism / Q000473:pathology*; D009919:Orchiectomy; D010751:Phosphopyruvate Hydratase / Q000032:analysis / Q000096:biosynthesis*; D011472:Prostatitis / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D053379:Chromogranin A; D004958:Estradiol; D010751:Phosphopyruvate Hydratase
keywords: Chromogranin A; chronic nonbacterial prostatitis; neuroendocrine; neuron-specific enolase