title: Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes.
abstract: Infection is the major reason for guided tissue regeneration/guided bone regeneration (GTR/GBR) membrane failure in clinical application. In this work, we developed GTR/GBR membranes with localized drug delivery function to prevent infection by electrospinning of poly(ε-caprolactone) (PCL) and gelatin blended with metronidazole (MNA). Acetic acid (HAc) was introduced to improve the miscibility of PCL and gelatin to fabricate homogeneous hybrid nanofiber membranes. The effects of the addition of HAc and the MNA content (0, 1, 5, 10, 20, 30, and 40 wt.% of polymer) on the properties of the membranes were investigated. The membranes showed good mechanical properties, appropriate biodegradation rate and barrier function. The controlled and sustained release of MNA from the membranes significantly prevented the colonization of anaerobic bacteria. Cells could adhere to and proliferate on the membranes without cytotoxicity until the MNA content reached 30%. Subcutaneous implantation in rabbits for 8 months demonstrated that MNA-loaded membranes evoked a less severe inflammatory response depending on the dose of MNA than bare membranes. The biodegradation time of the membranes was appropriate for tissue regeneration. These results indicated the potential for using MNA-loaded PCL/gelatin electrospun membranes as anti-infective GTR/GBR membranes to optimize clinical application of GTR/GBR strategies.
authors.affiliation: Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Xue
authors.affiliation: Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.affiliation: DaBeiNong Group (DBN) Research Center for Animal Medicine, Beijing 100195, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.affiliation: Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Yuzhao
authors.initials: Y
authors.lastname: Niu
authors.affiliation: Centre for Biomaterials and Tissue Engineering, University of Sheffield, Sheffield, South Yorkshire S3 7HQ, UK.
authors.forename: Aileen
authors.initials: A
authors.lastname: Crawford
authors.affiliation: School of Engineering, Design & Technology, University of Bradford, Bradford, West Yorkshire BD7 1DP, UK.
authors.forename: Phil D
authors.initials: PD
authors.lastname: Coates
authors.affiliation: Laboratory of Bone Tissue Engineering of Beijing Research Institute of Traumatology and Orthopaedics, Beijing 100035, China.
authors.forename: Dafu
authors.initials: D
authors.lastname: Chen
authors.affiliation: Laboratory of Bone Tissue Engineering of Beijing Research Institute of Traumatology and Orthopaedics, Beijing 100035, China. Electronic address: sharell@126.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Shi
authors.affiliation: Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address: zhanglq@mail.buct.edu.cn.
authors.forename: Liqun
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry; D001861:Bone Regeneration / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D016503:Drug Delivery Systems; D016967:Fusobacterium nucleatum / Q000187:drug effects; D005780:Gelatin / Q000737:chemistry*; D048091:Guided Tissue Regeneration / Q000379:methods; D008297:Male; D008566:Membranes / Q000737:chemistry*; D008795:Metronidazole / Q000494:pharmacology; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry; D011817:Rabbits
chemical_list: D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D011091:Polyesters; D008795:Metronidazole; C016240:polycaprolactone; D005780:Gelatin
keywords: Anti-infection; Drug delivery; Electrospinning; Guided tissue regeneration