title: Quantification of periodontal pathogens cell counts by capillary electrophoresis.
abstract: Gingivitis is a highly prevalent periodontal disease around the worldwide. Porphyromonas gingivalis (P.g), Treponema denticola (T.d) and Tannerela forsythia (T.f) were considered to be three important periodontal pathogens related to gingivitis, and research shows that the counts of periodontal pathogen cells in the patients before, during, and after fixed orthodontic appliance therapy were quite different. We proposed a simple method to extract the periodontal pathogens from the periodontal pocket in this work and demonstrated a new approach to determine periodontal pathogen level based on capillary electrophoresis (CE). After polymerase chain reaction amplification of P.g (197 bp), T.d (311 bp), and T.f (641 bp), it shows that they can rapidly identified by CE within 5 min. The peak area in the eletropherogram is linearly related to the concentration of P.g, T.d, and T.f, and the correlation coefficients R(2) corresponding to them are 0.993, 0.993, and 0.956, respectively. According to this linearly relationship, the estimated concentration of P.g, T.d, and T.f in gingival crevicular fluid from one volunteer was inferred to be about 9.90×10(2), 1.48×10(3), and 9.01×10(2)cells/μl, respectively.
authors.affiliation: Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai 200093, China. Electronic address: zhenqingli@163.com.
authors.forename: Zhenqing
authors.initials: Z
authors.lastname: Li
authors.affiliation: Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai 200093, China.
authors.forename: Shaoxiong
authors.initials: S
authors.lastname: Chen
authors.affiliation: Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai 200093, China.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Liu
authors.affiliation: Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai 200093, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Photonics and Bio-medical Research Institute, Department of Physics Faculty of Science, East China University of Science and Technology, No. 130 Meilong Road, Shanghai 200237, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Dou
authors.affiliation: Photonics and Bio-medical Research Institute, Department of Physics Faculty of Science, East China University of Science and Technology, No. 130 Meilong Road, Shanghai 200237, China; Department of Applied Physics, Graduate School of Engineering, Osaka University, Yamadaoka Suita-city, Osaka 565-0871, Japan; Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai 200093, China. Electronic address: yoshi.yamaguchi@ap.eng.osaka-u.ac.jp.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Yamaguchi
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D050296:Microbial Viability; D010514:Periodontal Pocket / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000166:cytology* / Q000235:genetics; D048373:Treponema denticola / Q000166:cytology* / Q000235:genetics
keywords: Analytical methods; Capillary electrophoresis; Fluorescence intensity; Periodontal pathogens; Polymerase chain reaction