title: Overproduction of geranylgeraniol in Coprinopsis cinerea by the expression of geranylgeranyl diphosphate synthase gene.
abstract: (E, E, E)-Geranylgeraniol (GGOH) is a valuable ingredient of many perfumes and a valuable precursor for synthesizing pharmaceuticals. In an attempt to increase the GGOH concentration in Coprinopsis cinerea, we demonstrated that the expression of geranylgeranyl diphosphate synthase (ggpps) gene isolated from Taxus x media could promote GGOH production. Furthermore, the concentrations of squalene and ergosterol were measured in the engineered strains. Expectedly, significant decreases of squalene and ergosterol levels were observed in those strains transformed with ggpps gene. This could be explained by the partial redirection of metabolic flux from squalene to GGOH, whose biosynthesis competes for the same precursor with squalene. This work suggested that the expression of ggpps in higher fungi was an effective method for bio-production of GGOH.
authors.affiliation: Department of Bioengineering, College of Food Science, South China Agricultural University, Guangzhou, China.
authors.forename: Lin-Feng
authors.initials: LF
authors.lastname: You
authors.forename: Li-Qiong
authors.initials: LQ
authors.lastname: Guo
authors.forename: Jun-Fang
authors.initials: JF
authors.lastname: Lin
authors.forename: Tao
authors.initials: T
authors.lastname: Ren
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Wang
mesh_terms: D001487:Basidiomycota / Q000235:genetics / Q000378:metabolism*; D004224:Diterpenes / Q000378:metabolism*; D004875:Ergosterol / Q000378:metabolism; D051231:Farnesyltranstransferase / Q000235:genetics* / Q000378:metabolism; D013185:Squalene / Q000378:metabolism; D020946:Taxus / Q000201:enzymology; D014170:Transformation, Genetic
chemical_list: D004224:Diterpenes; D013185:Squalene; C017338:geranylgeraniol; D051231:Farnesyltranstransferase; D004875:Ergosterol
keywords: Coprinopsis cinerea; Ergosterol; Geranylgeraniol; Geranylgeranyl diphosphate synthase; Squalene