title: Helicobacter pyloriinfection promotes the invasion and metastasis of gastric cancer through increasing the expression of matrix metalloproteinase-1 and matrix metalloproteinase-10.
abstract: The aim of this study was to investigate the effect and mechanism ofHelicobacter pyloriinfection in the invasion and metastasis of gastric cancer. Specimens from 80 patients with gastric cancer (of which 20 patients had metastatic gastric cancer) and 40 patients with chronic gastritis were included in this study.H. pyloriinfection was determined by ELISA and the expression of matrix metalloproteinase-1 (MMP-1) and MMP-10 was observed using immunohistochemistry. The correlation betweenH. pyloriinfection and the clinical pathological features of gastric cancer was analyzed by SPSS 13.0 software. The protein expression levels of MMP-1 and MMP-10 in MGC-803 cells infected withH. pyloriwere analyzed using western blotting.H. pyloriinfection was found in 62 of the 80 patients with gastric cancer and in 13 of the 40 patients with chronic gastritis. In addition,H. pyloriinfection was correlated with the staging and lymph node metastasis, but not with the gender, age and histological types of patients.H. pyloriinfection was also significantly correlated with the expression of MMP-1 and MMP-10 (r=0.8718, P<0.05 and r=0.5477, P<0.05, respectively). The expression of MMP-1 and MMP-10 was significantly upregulated following induction byH. pyloriinfection (P<0.05), with significant effects occurring following infection for 12 and 6 h, respectively.H. pyloriinfection may promote the invasion and metastasis of gastric cancer by increasing the expression of MMP-1 and MMP-10.
authors.affiliation: Gastric Cancer Research Center of Hunan, Department of Oncology, The First Affiliated Hospital, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Qianjin
authors.initials: Q
authors.lastname: Liao
authors.affiliation: Gastric Cancer Research Center of Hunan, Department of Oncology, The First Affiliated Hospital, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Ouyang
keywords: Helicobacter pylori; gastric cancer; matrix metalloproteinase-1; matrix metalloproteinase-10; metastasis