title: Trans-regulation of oligodendrocyte myelination by neurons through small GTPase Arf6-regulated secretion of fibroblast growth factor-2.
abstract: The small G protein ADP-ribosylation factor 6 (Arf6) plays important roles in a wide variety of membrane dynamics-based cellular events such as neurite outgrowth and spine formation in vitro. However, little is known about physiological function of Arf6 in vivo. Here we generate conditional knockout mice lacking Arf6 in neurons, oligodendrocytes, or both cell lineages, and unexpectedly find that Arf6 expression in neurons, but not in oligodendrocytes, is crucial for oligodendrocyte myelination in the hippocampal fimbria and the corpus callosum during development, and that this is through the regulation of secretion of fibroblast growth factor-2, a guidance factor for migration of oligodendrocyte precursor cells (OPCs). These results suggest that Arf6 in neurons plays an important role in OPC migration through regulation of FGF-2 secretion during neuronal development.
authors.affiliation: 1] Faculty of Medicine, Department of Physiological Chemistry, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan [2].
authors.forename: Masahiro
authors.initials: M
authors.lastname: Akiyama
authors.affiliation: 1] Faculty of Medicine, Department of Physiological Chemistry, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan [2].
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hasegawa
authors.affiliation: 1] Faculty of Medicine, Department of Physiological Chemistry, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan [2].
authors.forename: Tsunaki
authors.initials: T
authors.lastname: Hongu
authors.affiliation: Department of Pharmacology, Center for Developmental Genetics, Stony Brook University, Stony Brook, New York 11794-5140, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Frohman
authors.affiliation: Department of Cell Biology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Harada
authors.affiliation: Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Kanagawa 252-0329, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sakagami
authors.affiliation: Faculty of Medicine, Department of Physiological Chemistry, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Kanaho
mesh_terms: D000089982:ADP-Ribosylation Factor 6; D020727:ADP-Ribosylation Factors / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001369:Axons / Q000502:physiology; D002465:Cell Movement; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009186:Myelin Sheath / Q000378:metabolism; D009474:Neurons; D009836:Oligodendroglia / Q000166:cytology / Q000502:physiology*
chemical_list: D000089982:ADP-Ribosylation Factor 6; D016222:Fibroblast Growth Factor 2; D020727:ADP-Ribosylation Factors; C000715348:ARF6 protein, human; C000715727:Arf6 protein, mouse