title: A Metabonomics Profiling Study on Phlegm Syndrome and Blood-Stasis Syndrome in Coronary Heart Disease Patients Using Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry.
abstract: A metabonomics approach based on liquid chromatography/quadrupole time-of-flight mass spectrometry (LC-Q-TOF/MS) was utilized to obtain potential biomarkers of coronary heart disease (CHD) patients and investigate the ZHENG types differentiation in CHD patients. The plasma samples of 20 CHD patients with phlegm syndrome, 20 CHD patients with blood-stasis syndrome, and 16 healthy volunteers were collected in the study. 26 potential biomarkers were identified in the plasma of CHD patients and 19 differential metabolites contributed to the discrimination of phlegm syndrome and blood-stasis syndrome in CHD patients (VIP > 1.5; P < 0.05) which mainly involved purine metabolism, pyrimidine metabolism, amino acid metabolism, steroid biosynthesis, and arachidonic acid metabolism. This study demonstrated that metabonomics approach based on LC-MS was useful for studying pathologic changes of CHD patients and interpreting the differentiation of ZHENG types (phlegm and blood-stasis syndrome) in traditional Chinese medicine (TCM).
authors.affiliation: Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central south University, Changsha 410008, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central south University, Changsha 410008, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wan
authors.affiliation: Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central south University, Changsha 410008, China.
authors.forename: Xinjian
authors.initials: X
authors.lastname: Qiu
authors.affiliation: Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central south University, Changsha 410008, China.
authors.forename: Ruomeng
authors.initials: R
authors.lastname: Li
authors.affiliation: Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central south University, Changsha 410008, China.
authors.forename: Shimi
authors.initials: S
authors.lastname: Liu
authors.affiliation: Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central south University, Changsha 410008, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Wang