title: Rapid separation and characterization of diterpenoid alkaloids in processed roots of Aconitum carmichaeli using ultra high performance liquid chromatography coupled with hybrid linear ion trap-Orbitrap tandem mass spectrometry.
abstract: The lateral root of Aconitum carmichaeli, a popular traditional Chinese medicine, has been widely used to treat rheumatic diseases. For decades, diterpenoid alkaloids have dominated the phytochemical and biomedical research on this plant. In this study, a rapid and sensitive method based on ultra high performance liquid chromatography coupled with linear ion trap-Orbitrap tandem mass spectrometry was developed to characterize the diterpenoid alkaloids in Aconitum carmichaeli. Based on an optimized chromatographic condition, more than 120 diterpenoid alkaloids were separated with good resolution. Using a systematic strategy that combines high resolution separation, highly accurate mass measurements and a good understanding of the diagnostic fragment-based fragmentation patterns, these diterpenoid alkaloids were identified or tentatively identified. The identification of these chemicals provided essential data for further phytochemical studies and toxicity research of Aconitum carmichaeli. Moreover, the ultra high performance liquid chromatography with linear ion trap-Orbitrap mass spectrometry platform was an effective and accurate tool for rapid qualitative analysis of secondary metabolite productions from natural resources.
authors.affiliation: Lab of Chinese Materia Medica Preparation, the Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Dayuan
authors.initials: D
authors.lastname: Zhu
authors.forename: Juan
authors.initials: J
authors.lastname: Huang
authors.forename: Zhihai
authors.initials: Z
authors.lastname: Huang
authors.forename: Junqi
authors.initials: J
authors.lastname: Bai
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Qiu
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004224:Diterpenes / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000470:Alkaloids; D004224:Diterpenes
keywords: Aconitum carmichaeli; Diterpenoid alkaloids; Liquid chromatography with mass spectrometry