title: Structural studies of virus-antibody immune complexes (poliovirus type I): Characterization of the epitopes in 3D.
abstract: The inactivated polio vaccine (IPV) contains poliovirus (PVs) samples that belong to serotypes 1, 2 and 3. All three serotypes contain the D-antigen, which induces protective antibodies. The antigenic structure of PVs consists of at least four different antigenic sites and the D-antigen content represents the combined activity of multiple epitopes (Ferguson et al., 1993; Minor, 1990; Minor et al., 1986). The potency of IPV vaccines is determined by measuring the D-antigen content. Several ELISA methods have been developed using polyclonal or monoclonal antibodies (Mabs) in order to quantify the D-antigen content. Characterization of the epitopes recognized by the different Mabs is crucial to map the entire virus surface and ensure the presence of epitopes able to induce neutralizing antibodies. In a new approach, combining cryo-electron microscopy and image analysis with X-ray crystallography data available along with identification of exposed amino acids we have mapped in 3D the epitope sites recognized by five specific Fabs and one Mab and characterized precisely the antigenic sites for these Mabs. We propose this method to be used to map the entire "epitopic" surface of virus.
authors.affiliation: IMPMC, UMR 7590, CNRS-UPMC-IRD-MNHN, 75252 Paris, France.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Bannwarth
authors.affiliation: Sanofi Pasteur, 1541 av. Marcel Mérieux, F-69280 Marcy l'étoile, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Girerd-Chambaz
authors.affiliation: IMPMC, UMR 7590, CNRS-UPMC-IRD-MNHN, 75252 Paris, France.
authors.forename: Ana A
authors.initials: AA
authors.lastname: Arteni
authors.affiliation: IMPMC, UMR 7590, CNRS-UPMC-IRD-MNHN, 75252 Paris, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Guigner
authors.affiliation: IMPMC, UMR 7590, CNRS-UPMC-IRD-MNHN, 75252 Paris, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Lemains
authors.affiliation: Sanofi Pasteur, 1541 av. Marcel Mérieux, F-69280 Marcy l'étoile, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Ronzon
authors.affiliation: Sanofi Pasteur, 1541 av. Marcel Mérieux, F-69280 Marcy l'étoile, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Manin
authors.affiliation: IMPMC, UMR 7590, CNRS-UPMC-IRD-MNHN, 75252 Paris, France. Electronic address: catherine.venien@impmc.upmc.fr.
authors.forename: Catherine
authors.initials: C
authors.lastname: Vénien-Bryan
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D000914:Antibodies, Viral / Q000737:chemistry / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000737:chemistry* / Q000276:immunology*; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology; D020285:Cryoelectron Microscopy; D018360:Crystallography, X-Ray; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology*; D021621:Imaging, Three-Dimensional; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000276:immunology; D008958:Models, Molecular; D017955:Poliovirus / Q000737:chemistry* / Q000276:immunology* / Q000648:ultrastructure; D023302:Protein Footprinting
chemical_list: D000596:Amino Acids; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments
keywords: Antigenic site; Cryo-electron microscopy; Image analysis; Monoclonal antibody; Polio vaccine; Poliovirus type 1