title: A Nile Red/BODIPY-based bimodal probe sensitive to changes in the micropolarity and microviscosity of the endoplasmic reticulum.
abstract: We herein report a fluorescent bimodal probe (1) capable of determining ER viscosity and polarity changes using FLIM and fluorescence ratiometry, respectively; during ER stress caused by tunicamycin, the viscosity was increased from ca. 129.5 to 182.0 cP and the polarity of the ER (dielectric constant, ε) enhanced from 18.5 to 21.1.
authors.affiliation: Department of Chemistry, Korea University, Seoul 136-701, Korea. jongskim@korea.ac.kr.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Yang
authors.forename: Yanxia
authors.initials: Y
authors.lastname: He
authors.forename: Jae Hong
authors.initials: JH
authors.lastname: Lee
authors.forename: Weon-Sik
authors.initials: WS
authors.lastname: Chae
authors.forename: Wen Xiu
authors.initials: WX
authors.lastname: Ren
authors.forename: Joung Hae
authors.initials: JH
authors.lastname: Lee
authors.forename: Chulhun
authors.initials: C
authors.lastname: Kang
authors.forename: Jong Seung
authors.initials: JS
authors.lastname: Kim
mesh_terms: D001896:Boron Compounds / Q000737:chemistry / Q000494:pharmacology*; D016764:Cell Polarity; D004721:Endoplasmic Reticulum / Q000502:physiology*; D059865:Endoplasmic Reticulum Stress; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D010078:Oxazines / Q000737:chemistry / Q000494:pharmacology*; D014415:Tunicamycin / Q000494:pharmacology; D014783:Viscosity
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D010078:Oxazines; D014415:Tunicamycin; C044808:nile red