title: Molecularly imprinted polymer as in-line concentrator in capillary electrophoresis coupled with mass spectrometry for the determination of quinolones in bovine milk samples.
abstract: In this work molecularly imprinted polymers have been evaluated as sorbent for the construction of an in-line solid phase extraction analyte concentrator in capillary electrophoresis coupled with mass spectrometry for the determination of the eight regulated veterinary quinolones in bovine milk samples. Different parameters affecting the analyte concentrator performance, such as sample pH, volume and composition of the elution plug and injection time, were studied. Sample volumes of 22μL (2bar for 15min) were loaded on the MISPE microcartridge and the retained analytes were eluted by injecting a plug of MeOH/H2O/NH3 (60/37/3 by volume) for 125s at 50mbar (60nL). The proposed method is simple for the monitoring of these antibiotic residues in milk samples, allowing the direct injection of the samples with minimum sample pretreatment, achieving limits of detection between 3.8 and 4.7μgkg(-1) and unequivocal identification of the compounds working in tandem mass spectrometry. Recoveries ranging from 70.0 to 102.3% were obtained and satisfactory intra-day and inter-day RSDs were achieved (≤12% and 15% respectively). Reproducibility among different constructed analyte concentrators showed RSD≤11%.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Av. Fuentenueva s/n, 18071 Granada, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Moreno-González
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Av. Fuentenueva s/n, 18071 Granada, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Lara
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Av. Fuentenueva s/n, 18071 Granada, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Gámiz-Gracia
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Av. Fuentenueva s/n, 18071 Granada, Spain. Electronic address: amgarcia@ugr.es.
authors.forename: Ana M
authors.initials: AM
authors.lastname: García-Campaña
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D002138:Calibration; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008892:Milk / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods; D011108:Polymers / Q000737:chemistry; D015363:Quinolones / Q000032:analysis*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D011108:Polymers; D015363:Quinolones
keywords: Bovine milk samples; Capillary electrophoresis; In-line concentrator; Molecularly imprinted polymers; Quinolones