title: [Inhibitory effect of genistein on the proliferation of Raji cells and its related mechanism].
abstract: This study was aimed to investigate the anti-proliferative effect of genistein (Gen) on BCL-6 positive Raji cells and its related mechanism. Trypan blue staining and MTT method were used to analyze the anti-proliferative effect of Gen on Raji cells. Cell apoptosis, protein expression and the interaction of BCL-6 and NCoR were detected by PI/AV dual staining, Western blot and Co-IP method, respectively. The results showed that Gen had time- and dose-dependent inhibitory effect on Raji cell proliferation and induced apoptosis. Different dose of Gen had no significant effect on the expression of BCL-6 and NCoR, but could inhibit the binding of BCL-6 and NCoR. It is concluded that Gen shows inhibitory effect on BCL-6 positive lymphoma cells, which can be as a adjuvant therapy for combined rituximab with chemotherapy.
authors.affiliation: Department of Hematology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing 100050, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shen
authors.affiliation: Department of Hematology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing 100050, China.
authors.forename: Jing-Shi
authors.initials: JS
authors.lastname: Wang
authors.affiliation: Department of Hematology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing 100050, China.
authors.forename: Li
authors.initials: L
authors.lastname: Fu
authors.affiliation: Department of Hematology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing 100050, China.
authors.forename: Da-Yong
authors.initials: DY
authors.lastname: Huang
authors.affiliation: Department of Hematology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing 100050, China. E-mail: zhaowww263@yahoo.com.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D056971:Nuclear Receptor Co-Repressor 1 / Q000378:metabolism; D051560:Proto-Oncogene Proteins c-bcl-6 / Q000378:metabolism
chemical_list: C539127:NCOR1 protein, human; D056971:Nuclear Receptor Co-Repressor 1; D051560:Proto-Oncogene Proteins c-bcl-6; D019833:Genistein