title: Low expression of microRNA-30c promotes invasion by inducing epithelial mesenchymal transition in non-small cell lung cancer.
abstract: MicroRNA (miR)‑30c has been identified as a tumor suppressor gene in numerous diseases. Aberrant miR‑30c expression has been associated with the invasion of different types of cancer. However, the potential mechanisms underlying the association between miR‑30c and invasion has been poorly elucidated in non‑small‑cell lung cancer (NSCLC). In the present study, quantitative polymerase chain reaction demonstrated that the expression of miR‑30c was reduced in lung cancer specimens (n=85). Suppressing the expression of miR‑30c promoted the invasion of A549 cells, while overexpressed miR‑30c inhibited the invasion of A549 cells. Furthermore, aberrant miR‑30c expression was able to control the expression levels of markers (E‑cadherin, snail and vimentin) of epithelial mesenchymal transition (EMT). In conclusion, miR‑30c regulated the invasion of NSCLC cells and low miR-30 levels induced EMT.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, P.R. China.
authors.forename: Zhaopeng
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, P.R. China.
authors.forename: Pengli
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, P.R. China.
authors.forename: Yijiang
authors.initials: Y
authors.lastname: Chen
mesh_terms: D015703:Antigens, CD; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014746:Vimentin / Q000235:genetics / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D014746:Vimentin