title: Organo-modified layered double hydroxide-catalyzed Fenton-like ultra-weak chemiluminescence for specific sensing of vitamin B₁₂ in egg yolks.
abstract: In general, the chemiluminescence (CL) sensing of vitamin B12 is achieved by determining Co(II) liberated from acidified vitamin B12 by a luminol system. However, the luminol system for sensing vitamin B12 has poor selectivity due to serious interference from other metal ions. In this study, as a novel CL amplifier of the Co(II)+H2O2+OH(-) ultra-weak CL reaction (Fenton-like system), dodecylbenzene sulfonate (DBS)-layered double hydroxides (LDHs) have been applied to the specific determination of vitamin B12 by liberating Co(II). The CL intensity increased with increasing the concentration of vitamin B12 in a wide range from 1.0 ng mL(-1) to 5 μg mL(-1) with a detection limit of 0.57 ng mL(-1) (S/N=3). The proposed method has been successfully applied to determine vitamin B12 in egg yolk with simple procedures, shorter time and higher selectivity. Recoveries from spiked real samples were 96-103%. The results of the proposed method for sensing vitamin B12 in real samples were agreed with those obtained by the standard inductively coupled plasma mass spectrometry (ICP-MS) method. To the best of our knowledge, this is the first report on the CL sensing of vitamin B12 with high selectivity in the absence of luminol.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Wanqi
authors.initials: W
authors.lastname: Rong
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address: luchao@mail.buct.edu.cn.
authors.forename: Chao
authors.initials: C
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Zhao
mesh_terms: D000535:Aluminum / Q000737:chemistry; D000818:Animals; D001557:Benzenesulfonates / Q000737:chemistry; D003035:Cobalt / Q000737:chemistry; D003102:Colloids / Q000737:chemistry; D004530:Egg Yolk / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006878:Hydroxides / Q000737:chemistry*; D017665:Hydroxyl Radical; D007477:Ions; D007501:Iron / Q000737:chemistry*; D007770:L-Lactate Dehydrogenase / Q000737:chemistry; D008165:Luminol / Q000737:chemistry*; D008274:Magnesium / Q000737:chemistry; D013058:Mass Spectrometry; D008670:Metals / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D014805:Vitamin B 12 / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D001557:Benzenesulfonates; D003102:Colloids; C045076:Fenton's reagent; D006878:Hydroxides; D007477:Ions; D008670:Metals; D017665:Hydroxyl Radical; D003035:Cobalt; D008165:Luminol; C001114:dodecylbenzenesulfonic acid; D006861:Hydrogen Peroxide; D000535:Aluminum; D007501:Iron; D007770:L-Lactate Dehydrogenase; D008274:Magnesium; D014805:Vitamin B 12
keywords: Chemiluminescence; Fenton-like reaction; Organo-modified layered double hydroxides; Vitamin B(12)