title: Impaired erythrocyte deformability in transgenic HO-1G143H mutant mice.
abstract: To investigate the potential effects of variation of HO-1 activity on hemorheology, this study compared the hemorheological properties between transgenic HO-1G143H mutant mice and wild-type (WT) control mice. Fresh blood samples were obtained from mice via the ocular venous sinus. The whole blood viscosity was measured using a cone-plate viscometer. Erythrocyte deformability and aggregation was measured using ektacytometry. The elongation index was significantly reduced in the HO-1G143H mutant mice compared to the WT mice at the shear rates of 600, 800, and 1,000 s(-1). The integrated elongation index was decreased in the HO-1G143H mutant mice compared to the WT mice. There was no statistically significant difference between the HO-1G143H mutant mice and the WT mice in terms of whole blood viscosity, aggregation index, amplitude of aggregation, and aggregation half time. The present study demonstrated that a reduction in HO-1 activity results in an impaired erythrocyte deformability. Although the mechanism underlying this effect remains unclear, our study brings to light the participation of HO-1 in the variations of hemorheology.
authors.affiliation: Institute of Transfusion Medicine, Academy of Military Medical Sciences, No. 27th Taiping Road, HaiDian, Beijing, China.
authors.forename: Gan
authors.initials: G
authors.lastname: Chen
authors.forename: Yujing
authors.initials: Y
authors.lastname: Yin
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Penglong
authors.initials: P
authors.lastname: Li
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Liu
authors.forename: Guoxing
authors.initials: G
authors.lastname: You
authors.forename: Jingxiang
authors.initials: J
authors.lastname: Zhao
authors.forename: Sha
authors.initials: S
authors.lastname: Xia
authors.forename: Lian
authors.initials: L
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000818:Animals; D004903:Erythrocyte Aggregation; D004907:Erythrocyte Deformability / Q000235:genetics*; D018056:Hemorheology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic