title: Exopolysaccharide from Ganoderma applanatum as a promising bioactive compound with cytostatic and antibacterial properties.
abstract: A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungus Ganoderma applanatum (GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect. The results indicate that the tested GpEPS (at concentrations above 22.85 µg/mL and 228.5 µg/mL) may exhibit selective activity against tumor cells (cell lines SiHa) and stimulate production of TNF-α THP-1-derived macrophages at the level of 752.17 pg/mL. The GpEPS showed antibacterial properties against Staphyloccoccus aureus and a toxic effect against Vibrio fischeri cells (82.8% cell damage). High cholesterol-binding capacity and triglycerides-binding capacity (57.9% and 41.6% after 24 h of incubation with the tested substances, resp.) were also detected for the investigated samples of GpEPS.
authors.affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Osińska-Jaroszuk
authors.affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Magdalena
authors.identifier: 0000-0003-2609-1820
authors.initials: M
authors.lastname: Jaszek
authors.affiliation: Department of Virology and Immunology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Magdalena
authors.identifier: 0000-0003-2364-6181
authors.initials: M
authors.lastname: Mizerska-Dudka
authors.affiliation: Department of Virology and Immunology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Adriana
authors.initials: A
authors.lastname: Błachowicz
authors.affiliation: Department of Virology and Immunology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Tomasz Piotr
authors.identifier: 0000-0003-1822-2181
authors.initials: TP
authors.lastname: Rejczak
authors.affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Grzegorz
authors.identifier: 0000-0002-2046-2806
authors.initials: G
authors.lastname: Janusz
authors.affiliation: Department of Comparative Anatomy and Anthropology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Wydrych
authors.affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Polak
authors.affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Jarosz-Wilkołazka
authors.affiliation: Department of Virology and Immunology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Martyna
authors.initials: M
authors.lastname: Kandefer-Szerszeń
mesh_terms: D048908:Aliivibrio fischeri / Q000187:drug effects; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D054697:Cytostatic Agents / Q000737:chemistry / Q000494:pharmacology*; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D032902:Ganoderma / Q000737:chemistry*; D006801:Humans; D007155:Immunologic Factors; D050296:Microbial Viability / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D054697:Cytostatic Agents; D062610:Fungal Polysaccharides; D007155:Immunologic Factors