title: Differential detection of Rhizoma coptidis by capillary electrophoresis electrospray ionization mass spectrometry with a nanospray interface.
abstract: A lab prototype CE-nanospray-MS platform with a high sensitivity porous sprayer was successfully applied in differential identification of Rhizoma coptidis in this paper. To obtain a stable and reliable nanospray, detailed optimizations about emitter geometry, buffer composition, emitter position, and spray voltage, as well as emitter cleanliness were discussed. Results showed that the reproducibility and sensitivity for separations of alkaloid standards were satisfactory using CE-nanospray-MS, which were also compared to ultra-HPLC (UHPLC)-MS. Their signal responds were at the same order of magnitude (intensities: 0.8 - 1.5 × 10(8) vs. 3.8 - 6.2 × 10(8) ), even though a 2 nL injection for CE was 2500-fold lower than UHPLC (5 μL injection). The absolute LOD results of CE-MS showed a remarkable superiority (18-24 fg), equal to 1000-fold lower than that of UHPLC-MS. Principal component analysis (PCA) of adulterated R. coptidis showed that this protocol had the ability to profile and qualify complex herb medicines, which also created a great potential for evaluation and qualification of rare and valuable Chinese medicines in future.
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry, Peking University, Beijing, P. R. China.
authors.forename: Jing-Xin
authors.initials: JX
authors.lastname: Liu
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Zhang
authors.forename: Fang
authors.initials: F
authors.lastname: Yuan
authors.forename: Hong-Xu
authors.initials: HX
authors.lastname: Chen
authors.forename: Xin-Xiang
authors.initials: XX
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D000089042:Coptis chinensis; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D036103:Nanotechnology / Q000295:instrumentation*; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; C107393:Coptidis rhizoma extract
keywords: CE-nanospary-MS; PCA; Rhizoma coptidis; TCM