title: Alginate based hybrid copolymer hydrogels--influence of pore morphology on cell-material interaction.
abstract: Alginate based hybrid copolymer hydrogels with unidirectional pore morphology were prepared to achieve synergistic biological performance for cardiac tissue engineering applications. Alginate based hybrid copolymer (ALGP) were prepared using alginate and poly(propylene fumarate) (HT-PPF) units. Different hybrid bimodal hydrogels were prepared by covalent crosslinking using poly(ethylene glycol diacrylate) and vinyl monomer viz acrylic acid, methyl methacrylate, butyl methacrylate and N-N'-methylene-bis-acrylamide and ionic crosslinking with calcium. The morphologically modified hydrogels (MM-hydrogels) with unidirectional elongated pores and high aspect ratio were prepared. MM-hydrogels favour better mechanical properties; it also enhances cell viability and infiltration due to unidirectional pores. However, the crosslinkers influence the fibroblast infiltration of these hydrogels. Synthesis of collagen and fibroblast infiltration was greater for alginate copolymer crosslinked with poly(ethylene glycol diacrylate-acrylic acid (ALGP-PA) even after one month (288%). This hybrid MM-hydrogel promoted cardiomyoblast growth on to their interstices signifying its potent applications in cardiac tissue engineering.
authors.affiliation: Sree ChitraTirunal Institute for Medical Sciences and Technology, Polymer Science Division, BMT Wing, Poojapura, Thiruvananthapuram 695 012, Kerala, India.
authors.forename: Finosh
authors.initials: F
authors.lastname: Gnanaprakasam Thankam
authors.affiliation: Sree ChitraTirunal Institute for Medical Sciences and Technology, Polymer Science Division, BMT Wing, Poojapura, Thiruvananthapuram 695 012, Kerala, India. Electronic address: mjayabalan52@gmail.com.
authors.forename: Jayabalan
authors.initials: J
authors.lastname: Muthu
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis; D049109:Cell Proliferation; D003094:Collagen / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology; D005650:Fumarates / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice; D032383:Myocytes, Cardiac / Q000166:cytology; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011126:Polypropylenes / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000178:Acrylamides; D000464:Alginates; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D005650:Fumarates; D006603:Hexuronic Acids; D020100:Hydrogels; D011108:Polymers; D011126:Polypropylenes; C437167:poly(ethylene glycol)diacrylate; C097850:poly(propylene fumarate); D011092:Polyethylene Glycols; D020723:Glucuronic Acid; D003094:Collagen; C021221:N,N'-methylenebisacrylamide
keywords: Alginate hybrid copolymer; Cell–material interaction; Morphologically modified hydrogels; Physical properties