title: Sirolimus influence on hepatectomy-induced liver regeneration in rats.
abstract: OBJECTIVE
  To evaluate the influence of sirolimus on liver regeneration triggered by resection of 70% of the liver of adult rats.
  
  
  METHODS
  we used 40 Wistar rats randomly divided into two groups (study and control), each group was divided into two equal subgroups according to the day of death (24 hours and seven days). Sirolimus was administered at a dose of 1mg/kg in the study group and the control group was given 1 ml of saline. The solutions were administered daily since three days before hepatectomy till the rats death to removal of the regenerated liver, conducted in 24 hours or 7 days after hepatectomy. Liver regeneration was measured by the KWON formula, by the number of mitotic figures (hematoxylin-eosin staining) and by the immunohistochemical markers PCNA and Ki-67.
  
  
  RESULTS
  there was a statistically significant difference between the 24h and the 7d groups. When comparing the study and control groups in the same period, there was a statistically significant variation only for Ki-67, in which there were increased numbers of hepatocytes in cell multiplication in the 7d study group compared with the 7d control group (p = 0.04).
  
  
  CONCLUSIONS
  there was no negative influence of sirolimus in liver regeneration and there was a positive partial effect at immunohistochemistry with Ki-67.
authors.affiliation: Department of Surgery, Universidade Federal do Paraná, Paraná, PR, Brazil.
authors.forename: Edimar Leandro
authors.initials: EL
authors.lastname: Toderke
authors.affiliation: Department of Surgery, Universidade Federal do Paraná, Paraná, PR, Brazil.
authors.forename: Giorgio Alfredo Pedroso
authors.initials: GA
authors.lastname: Baretta
authors.affiliation: Department of Surgery, Universidade Federal do Paraná, Paraná, PR, Brazil.
authors.forename: Ozimo Pereira
authors.initials: OP
authors.lastname: Gama Filho
authors.affiliation: Department of Surgery, Universidade Federal do Paraná, Paraná, PR, Brazil.
authors.forename: Jorge Eduardo Fouto
authors.initials: JE
authors.lastname: Matias
mesh_terms: D000818:Animals; D006498:Hepatectomy; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020123:Sirolimus / Q000494:pharmacology*
chemical_list: D007166:Immunosuppressive Agents; D020123:Sirolimus