title: Efficient utilization of complex N-linked glycans is a selective advantage for Bacteroides fragilis in extraintestinal infections.
abstract: Bacteroides fragilis is the most common anaerobe isolated from clinical infections, and in this report we demonstrate a characteristic of the species that is critical to their success as an opportunistic pathogen. Among the Bacteroides spp. in the gut, B. fragilis has the unique ability of efficiently harvesting complex N-linked glycans from the glycoproteins common to serum and serous fluid. This activity is mediated by an outer membrane protein complex designated as Don. Using the abundant serum glycoprotein transferrin as a model, it has been shown that B. fragilis alone can rapidly and efficiently deglycosylate this protein in vitro and that transferrin glycans can provide the sole source of carbon and energy for growth in defined media. We then showed that transferrin deglycosylation occurs in vivo when B. fragilis is propagated in the rat tissue cage model of extraintestinal growth, and that this ability provides a competitive advantage in vivo over strains lacking the don locus.
authors.affiliation: Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 27834.
authors.forename: Yanlu
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 27834.
authors.forename: Edson R
authors.initials: ER
authors.lastname: Rocha
authors.affiliation: Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 27834 smithcha@ecu.edu.
authors.forename: C Jeffrey
authors.initials: CJ
authors.lastname: Smith
mesh_terms: D000038:Abscess / Q000378:metabolism / Q000382:microbiology; D000693:Anaerobiosis; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001442:Bacteroides Infections / Q000378:metabolism* / Q000382:microbiology*; D001441:Bacteroides fragilis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001826:Body Fluids / Q000378:metabolism / Q000382:microbiology; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D015194:Diffusion Chambers, Culture / Q000382:microbiology; D004195:Disease Models, Animal; D005947:Glucose / Q000378:metabolism; D006023:Glycoproteins / Q000097:blood / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D064307:Microbiota; D010802:Phylogeny; D011134:Polysaccharides / Q000378:metabolism*; D051381:Rats; D013552:Swine; D014168:Transferrin / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D006023:Glycoproteins; D011134:Polysaccharides; D014168:Transferrin; D002244:Carbon; D005947:Glucose