title: Detection of antibodies against porcine epidemic diarrhea virus in serum and colostrum by indirect ELISA.
abstract: An indirect porcine epidemic diarrhea virus (PEDV) anti-immunoglobulin (Ig) G ELISA based on the S1 portion of the spike protein was validated and compared with an indirect immunofluorescence assay. In serum samples from experimentally infected pigs (n = 35), anti-IgG PEDV antibodies were detected as early as 7 days post-infection. In field serum samples (n = 239), the diagnostic sensitivity of the S1 ELISA was 100% and the diagnostic specificity was 94%. The S1 ELISA showed no cross-reactivity with antibodies against other porcine coronaviruses. Colostrum samples (n = 133) were also tested for anti-PEDV IgG and IgA. The diagnostic sensitivity was 92% for IgG and 100% for IgA, and the diagnostic specificity was 90% for IgG and 99.4% for IgA. These data suggest that the S1 ELISA is a sensitive and specific test that could also be used to evaluate PEDV colostral immunity.
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, USA.
authors.forename: Priscilla F
authors.initials: PF
authors.lastname: Gerber
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, USA.
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Gong
authors.affiliation: Institute of Preventive Veterinary Medicine and Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, China.
authors.forename: Yao-Wei
authors.initials: YW
authors.lastname: Huang
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, USA.
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, USA.
authors.forename: Derald
authors.initials: D
authors.lastname: Holtkamp
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, USA; The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Edinburgh, UK. Electronic address: tanja.opriessnig@roslin.ed.ac.uk.
authors.forename: Tanja
authors.initials: T
authors.lastname: Opriessnig
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D003126:Colostrum / Q000737:chemistry*; D018352:Coronavirus Infections / Q000097:blood / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D053485:Porcine epidemic diarrhea virus / Q000276:immunology*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000276:immunology / Q000821:virology*
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M
keywords: Diagnosis; ELISA; Porcine epidemic diarrhea virus; S1 protein; Serology