title: Does a short-term exposure to cadmium chloride affects haemocyte parameters of the marine gastropod Haliotis tuberculata?
abstract: In this study, a model based on primary cultured haemocytes from the gastropod mollusc Haliotis tuberculata was established to investigate the effects of cadmium chloride in vitro. Cells were exposed for 24 h to CdCl2 concentrations of 0, 1 and 100 μg ml(-1). The effects of cadmium on haemocyte parameters were investigated using morphological, spectrophotometric and flow cytometry analysis. Results showed that cadmium has no significant effects on cell viability and phagocytotic activity under the tested conditions. However, haemocytes became more rounded after cadmium exposure, which could explain the significant decrease of cell area beginning at 1 μg ml(-1) of CdCl2.
authors.affiliation: Marine Ecotoxicology, UR 09-03, IPEIS BP 805, 3018, Sfax, Tunisia. rladhar@yahoo.fr.
authors.forename: Rim
authors.initials: R
authors.lastname: Ladhar-Chaabouni
authors.affiliation: Marine Ecotoxicology, UR 09-03, IPEIS BP 805, 3018, Sfax, Tunisia.
authors.forename: Monia
authors.initials: M
authors.lastname: Machreki-Ajmi
authors.affiliation: Normandie Université, 14032, Caen, France.|CNRS INEE, FRE 3,484 BioMEA, SFR ICORE, Université de Caen Basse-Normandie, Esplanade de la Paix, 14032, Caen, Cedex, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Serpentini
authors.affiliation: Normandie Université, 14032, Caen, France.|CNRS INEE, FRE 3,484 BioMEA, SFR ICORE, Université de Caen Basse-Normandie, Esplanade de la Paix, 14032, Caen, Cedex, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Lebel
authors.affiliation: Marine Ecotoxicology, UR 09-03, IPEIS BP 805, 3018, Sfax, Tunisia.
authors.forename: Amel
authors.initials: A
authors.lastname: Hamza-Chaffai
mesh_terms: D000818:Animals; D019256:Cadmium Chloride / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D005434:Flow Cytometry; D049851:Gastropoda / Q000166:cytology* / Q000187:drug effects*; D006434:Hemocytes / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects; D013997:Time Factors
chemical_list: D004785:Environmental Pollutants; D019256:Cadmium Chloride
keywords: Cadmium chloride; Haemocytes; Haliotis tuberculata; Immune parameters