title: Proanthocyanidins from Grape Seeds Modulate the NF-κB Signal Transduction Pathways in Rats with TNBS-Induced Ulcerative Colitis.
abstract: To elucidate the molecular mechanisms involved in the therapeutic effects of proanthocyanidins from grape seeds (GSPE), we explore whether GSPE regulates the inflammatory response of TNBS-induced colitis in rats at the levels of NF-κB signal transduction pathway. Rats were intragastrically administered of different doses of GSPE (100, 200 and 400 mg·kg-1) per day for seven days after ulcerative colitis (UC) was induced by intracolonic injection of 2,4,6-trinitrobenzenesulfonic acid (TNBS) dissolved in 50% ethanol. Sulfasalazine (SASP) at 400 mg/kg was used as a positive control drug. The expression of nuclear factor-kappa B (NF-κB), phospho-I kappaB-alpha (pIκBα), inhibitor kappa B kinase (IκK) in the colon tissues were all measured by enzyme-linked immunosorbent assay (ELISA) methods. Treatment with GSPE reduced the expression of NF-κB, pIκBα and IκK in the colon. The results of this study show that GSPE exerts beneficial effects in inflammatory bowel disease by inhibition of NF-κB signal transduction pathways.
authors.affiliation: Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Department of College of Medicine, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Li
authors.affiliation: People's Hospital of Gansu Province, Lanzhou 730000, China.
authors.forename: Xiaolai
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Pharmacy, Southwestern Hospital, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Department of College of Medicine, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Qin
authors.affiliation: Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Department of College of Medicine, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: People's Hospital of Gansu Province, Lanzhou 730000, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Department of College of Medicine, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Department of College of Medicine, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Department of College of Medicine, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Yan
authors.affiliation: Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Department of College of Medicine, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Department of College of Medicine, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Department of College of Medicine, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
authors.forename: Wan
authors.initials: W
authors.lastname: Li
authors.affiliation: Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Department of College of Medicine, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
authors.forename: Sijia
authors.initials: S
authors.lastname: Li
authors.affiliation: Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Department of College of Medicine, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Department of College of Medicine, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China. wuyj@lzu.edu.cn.
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000818:Animals; D003093:Colitis, Ulcerative / Q000139:chemically induced* / Q000378:metabolism*; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D056604:Grape Seed Extract / Q000494:pharmacology*; D051550:I-kappa B Kinase / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D044945:Proanthocyanidins / Q000494:pharmacology*; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects*; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D056604:Grape Seed Extract; C511402:Grape Seed Proanthocyanidins; D053583:Interleukin-1beta; D016328:NF-kappa B; D044945:Proanthocyanidins; D014302:Trinitrobenzenesulfonic Acid; D009195:Peroxidase; D051550:I-kappa B Kinase