title: Comparative metabolite profiling of Solanum tuberosum against six wild Solanum species with Colorado potato beetle resistance.
abstract: The Colorado potato beetle Leptinotarsa decemlineata (Say) (CPB) is a coleopteran herbivore that feeds on the foliage on Solanum species, in particular, potato. Six resistant wild Solanum species were identified, and two of these species had low levels of glycoalkaloids. Comparative analysis of the untargeted metabolite profiles of the foliage using UPLC-qTOF-MS was done to find metabolites shared between the wild species but not with Solanum tuberosum (L.) to identify resistance-related metabolites. It was found that only S. tuberosum produced the triose glycoalkaloids solanine and chaconine. Instead, the six wild species produced glycoalkaloids that shared in common tetrose sugar side chains. Additionally, there were non-glycoalkaloid metabolites associated with resistance including hydroxycoumarin and a phenylpropanoid, which were produced in all wild species but not in S. tuberosum.
authors.affiliation: Agriculture and Agri-Food Canada Potato Research Centre, P.O. Box 20280, 850 Lincoln Road, Fredericton, New Brunswick, Canada E3B 4Z7.
authors.forename: Helen H
authors.initials: HH
authors.lastname: Tai
authors.forename: Kraig
authors.initials: K
authors.lastname: Worrall
authors.forename: Yvan
authors.initials: Y
authors.lastname: Pelletier
authors.forename: David
authors.initials: D
authors.lastname: De Koeyer
authors.forename: Larry A
authors.initials: LA
authors.lastname: Calhoun
mesh_terms: D000470:Alkaloids / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D001517:Coleoptera / Q000187:drug effects* / Q000502:physiology; D006027:Glycosides / Q000378:metabolism; D055432:Metabolomics; D010935:Plant Diseases; D018515:Plant Leaves / Q000737:chemistry* / Q000378:metabolism*; D012991:Solanaceous Alkaloids / Q000378:metabolism; D012992:Solanine / Q000378:metabolism / Q000494:pharmacology; D032322:Solanum / Q000254:growth & development / Q000378:metabolism*; D011198:Solanum tuberosum / Q000254:growth & development / Q000378:metabolism*; D014053:Tomatine / Q000378:metabolism
chemical_list: D000470:Alkaloids; D006027:Glycosides; D012991:Solanaceous Alkaloids; D012992:Solanine; D014053:Tomatine
keywords: Colorado potato beetle resistance; Solanum; glycoalkaloid; phenylpropanoid; potato; untargeted metabolite profiling