title: Copaiba oil effect under different pathways in mice subjected to sepsis.
abstract: OBJECTIVE
  To evaluate the effects of copaiba oil administered by different routes on survival of mices subjected to cecal ligation and puncture.
  
  
  METHODS
  Thirty two mice were distributed into four study groups (N=8): Sham group: normal standard animals;
  
  
  METHODS
  submitted a cecal ligation and puncture (CLP); Gavage group: submitted a CLP, and treat with copaiba oil by gavage; and Subcutaneous group: submitted a CLP, and treat with copaiba oil by subcutaneous injection. After the death of the histological analysis were performed. The Kaplan-Meier curves of surviving time were realized.
  
  
  RESULTS
  All animals that received copaiba, regardless of the route used, survived longer when compared to the control group (p<0.0001), whereas the survival time ranged from 20 hours for the control group up to 32 hours for the animals of gavage group and 52 for subcutaneous group. The animals that received gavage copaiba lived about and about 20 hours unless the subcutaneous group (p=0.0042). There was no statistical difference when compared the intensity of inflammatory response (p>0.05) CONCLUSION: Prophylactic subcutaneous administration of copaiba in mice subjected to severe sepsis by cecal ligation and puncture, resulted in a survival time higher than non-use or use of this oil by gavage.
authors.affiliation: Department of Integrate Health, Medical School, University State of Para, Brazil.
authors.forename: Nara Macedo
authors.initials: NM
authors.lastname: Botelho
authors.affiliation: UFPA, Belem, PA, Brazil.
authors.forename: Edvaldo Lima
authors.initials: EL
authors.lastname: Silveira
authors.affiliation: School of Medicine, UEPA, Brazil.
authors.forename: Letícia Nobre
authors.initials: LN
authors.lastname: Lopes
authors.affiliation: School of Medicine, UEPA, Brazil.
authors.forename: Felipe Augusto Folha
authors.initials: FA
authors.lastname: Santos
authors.affiliation: School of Medicine, UEPA, Brazil.
authors.forename: Renan Kleber Costa
authors.initials: RK
authors.lastname: Teixeira
authors.affiliation: School of Medicine, UEPA, Brazil.
authors.forename: Thaís Travassos da
authors.initials: TT
authors.lastname: Silva
mesh_terms: D000818:Animals; D001453:Balsams / Q000008:administration & dosage* / Q000627:therapeutic use; D002432:Cecum / Q000601:surgery; D007279:Injections, Subcutaneous / Q000379:methods; D007441:Intubation, Gastrointestinal / Q000379:methods; D008026:Ligation; D008168:Lung / Q000473:pathology; D008297:Male; D051379:Mice; D023421:Models, Animal; D009336:Necrosis / Q000473:pathology; D010532:Peritoneal Diseases / Q000188:drug therapy; D011677:Punctures; D011897:Random Allocation; D018805:Sepsis / Q000188:drug therapy*; D016019:Survival Analysis; D013997:Time Factors
chemical_list: D001453:Balsams; C114971:copaiba balsam