title: Development of nanofluorapatite polymer-based composite for bioactive orthopedic implants and prostheses.
abstract: Fluorapatite with low solubility is a promising biomaterial due to its structure, which is similar to hydroxyapatite. In this study a bioactive composite of nanofluorapatite (n-FA) and polyamide 12 (PA12) was fabricated. The results revealed that the mechanical properties (such as compressive strength and elastic modulus), hydrophilicity, and antibacterial properties of n-FA/PA12 composite were obviously improved by adding n-FA into PA12 as compared with PA12. In addition, cell proliferation of MC3T3-E1 cells cultured on n-FA/PA12 composite was significantly higher than with PA12, and alkaline phosphatase activity of MC3T3-E1 cells on the n-FA/PA12 composite was expressed at obviously higher levels as compared with PA12. The results suggest that n-FA/PA12 composite could support cell proliferation and differentiation, showing good cytocompatibility. Histological evaluation indicates that n-FA/PA12 composite enhances the efficiency of new bone formation with the introduction of n-FA into PA12, and the quantity of the newly formed bone for n-FA/PA12 composite is significantly higher than with PA12. In conclusion, n-FA/PA12 composite exhibits good biocompatibility and osteogenesis, which might be used for various orthopedic prostheses and dental implants.
authors.affiliation: The First People's Hospital of Xiaoshang, Hangzhou, People's Republic of China ; Key Laboratory of Ultra-fine Materials, Ministry of Education, East China University of Science and Technology, Shanghai, People's Republic of China.
authors.forename: Gangfeng
authors.initials: G
authors.lastname: Hu
authors.affiliation: The First People's Hospital of Xiaoshang, Hangzhou, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: The First People's Hospital of Xiaoshang, Hangzhou, People's Republic of China.
authors.forename: Xiaocong
authors.initials: X
authors.lastname: Yao
authors.affiliation: The First People's Hospital of Xiaoshang, Hangzhou, People's Republic of China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Bi
authors.affiliation: The First People's Hospital of Xiaoshang, Hangzhou, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Ultra-fine Materials, Ministry of Education, East China University of Science and Technology, Shanghai, People's Republic of China.
authors.forename: Songchao
authors.initials: S
authors.lastname: Tang
authors.affiliation: Key Laboratory of Ultra-fine Materials, Ministry of Education, East China University of Science and Technology, Shanghai, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wei
authors.affiliation: Department of Orthopedic Surgery, Changzheng Hospital, The Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: Yang
authors.affiliation: Zhejiang Traditional Chinese Medical University, Hangzhou, People's Republic of China.
authors.forename: Peijian
authors.initials: P
authors.lastname: Tong
authors.affiliation: Zhejiang Traditional Chinese Medical University, Hangzhou, People's Republic of China.
authors.forename: Luwei
authors.initials: L
authors.lastname: Xiao
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001031:Apatites / Q000737:chemistry* / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D051379:Mice; D050296:Microbial Viability / Q000187:drug effects; D053761:Nanocomposites / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry*; D013499:Surface Properties; D054457:Tissue Scaffolds
chemical_list: D000900:Anti-Bacterial Agents; D001031:Apatites; D001672:Biocompatible Materials; D009757:Nylons; C036222:nylon 12; C025105:fluorapatite
keywords: bioactive composites; cell behavior; nanofluorapatite; orthopedic implants