title: PCR-based prediction of type I β-exotoxin production in Bacillus thuringiensis strains.
abstract: Some Bacillus thuringiensis strains secrete type I β-exotoxin, which is a non-specific insecticidal and thermostable adenine nucleoside oligosaccharide. Toxicity bioassays and HPLC are traditional methods for detecting β-exotoxin. With the aim of establish a first rapid approach for prediction of type I β-exotoxin production, two PCR-based methods were successfully evaluated in B. thuringiensis strains and native isolates. In order to validate a reliable technology, results obtained by this method were correlated with that obtained from Musca domestica bioassays.
authors.affiliation: Instituto de Microbiología y Zoología Agrícola INTA, CC No 25, 1712 Castelar, Buenos Aires, Argentina. Electronic address: sauka.diego@inta.gob.ar.
authors.forename: Diego H
authors.initials: DH
authors.lastname: Sauka
authors.affiliation: Instituto de Microbiología y Zoología Agrícola INTA, CC No 25, 1712 Castelar, Buenos Aires, Argentina.
authors.forename: Melisa P
authors.initials: MP
authors.lastname: Pérez
authors.affiliation: Instituto de Microbiología y Zoología Agrícola INTA, CC No 25, 1712 Castelar, Buenos Aires, Argentina.
authors.forename: Nanci N
authors.initials: NN
authors.lastname: López
authors.affiliation: Instituto de Microbiología y Zoología Agrícola INTA, CC No 25, 1712 Castelar, Buenos Aires, Argentina.
authors.forename: María I
authors.initials: MI
authors.lastname: Onco
authors.affiliation: Instituto de Microbiología y Zoología Agrícola INTA, CC No 25, 1712 Castelar, Buenos Aires, Argentina.
authors.forename: Marcelo F
authors.initials: MF
authors.lastname: Berretta
authors.affiliation: Instituto de Microbiología y Zoología Agrícola INTA, CC No 25, 1712 Castelar, Buenos Aires, Argentina.
authors.forename: Graciela B
authors.initials: GB
authors.lastname: Benintende
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000096:biosynthesis; D001413:Bacillus thuringiensis / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013400:Sugar Acids / Q000032:analysis*
chemical_list: D013400:Sugar Acids; C068295:thuringiensin; D000241:Adenosine
keywords: Bacillus thuringiensis; PCR; thuE gene; β-exotoxin