title: Molecular and phytochemical investigation of Angelica dahurica and Angelica pubescentis essential oils and their biological activity against Aedes aegypti, Stephanitis pyrioides, and Colletotrichum species.
abstract: In this study, Angelica dahurica and Angelica pubescentis root essential oils were investigated as pest management perspectives, and root samples were also analyzed genetically using the nuclear ribosomal internal transcribed spacer (ITS) region as a DNA barcode marker. A. pubescentis root essential oil demonstrated weak antifungal activity against Colletotrichum acutatum, Colletotrichum fragariae, and Colletotrichum gloeosporioides, whereas A. dahurica root essential oil did not show antifungal activity. Conversely, A. dahurica root essential oil demonstrated better biting deterrent and insecticidal activity against yellow fever mosquito, Aedes aegypti, and azalea lace bugs, Stephanitis pyrioides, than A. pubescentis root oil. The major compounds in the A. dahurica oil were found as α-pinene (46.3%), sabinene (9.3%), myrcene (5.5%), 1-dodecanol (5.2%), and terpinen-4-ol (4.9%). α-Pinene (37.6%), p-cymene (11.6%), limonene (8.7%), and cryptone (6.7%) were the major compounds found in the A. pubescentis oil. In mosquito bioassays, 1-dodecanol and 1-tridecanol showed antibiting deterrent activity similar to the positive control DEET (N,N-diethyl-3-methylbenzamide) at 25 nmol/cm(2) against Ae. aegypti, whereas only 1-tridecanol showed repellent activity in human-based cloth patch bioassay with minimum effective dosages (MED) of 0.086 ± 0.089 mg/cm(2) (DEET = 0.007 ± 0.003 mg/cm(2)). In larval bioassays, 1-tridecanol was more toxic with an LC50 value of 2.1 ppm than 1-dodecanol having an LC50 value of 5.2 ppm against 1-day-old Ae. aegypti larvae. 1-Dodecanol and 1-tridecanol could be useful for the natural mosquito control agents.
authors.affiliation: National Center for Natural Products Research, The University of Mississippi , University, Mississippi 38677, United States.
authors.forename: Nurhayat
authors.initials: N
authors.lastname: Tabanca
authors.forename: Zengping
authors.initials: Z
authors.lastname: Gao
authors.forename: Betul
authors.initials: B
authors.lastname: Demirci
authors.forename: Natascha
authors.initials: N
authors.lastname: Techen
authors.forename: David E
authors.initials: DE
authors.lastname: Wedge
authors.forename: Abbas
authors.initials: A
authors.lastname: Ali
authors.forename: Blair J
authors.initials: BJ
authors.lastname: Sampson
authors.forename: Chris
authors.initials: C
authors.lastname: Werle
authors.forename: Ulrich R
authors.initials: UR
authors.lastname: Bernier
authors.forename: Ikhlas A
authors.initials: IA
authors.lastname: Khan
authors.forename: Kemal Husnu Can
authors.initials: KH
authors.lastname: Baser
mesh_terms: D000330:Aedes / Q000187:drug effects / Q000254:growth & development; D029969:Angelica / Q000737:chemistry*; D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry* / Q000494:pharmacology*; D020231:Colletotrichum / Q000187:drug effects; D020062:Heteroptera / Q000187:drug effects; D007306:Insecticides / Q000737:chemistry* / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D007306:Insecticides; D009822:Oils, Volatile; D010938:Plant Oils