title: Identification of bromophenol thiohydantoin as an inhibitor of DisA, a c-di-AMP synthase, from a 1000 compound library, using the coralyne assay.
abstract: c-di-AMP is an important bacterial second messenger found in Gram-positive and mycobacteria. c-di-AMP regulates myriads of processes in bacteria as well as immune response in higher organisms so interest in small molecules that would attenuate the activity of c-di-AMP metabolism enzymes is high. Herein, we report the first small molecule inhibitor of a c-di-AMP synthase, DisA, using a coralyne-based assay.
authors.affiliation: Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, USA. hsintim@umd.edu.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zheng
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: David A
authors.initials: DA
authors.lastname: Sayre
authors.forename: Herman O
authors.initials: HO
authors.lastname: Sintim
mesh_terms: D001600:Berberine Alkaloids / Q000032:analysis / Q000737:chemistry*; D015226:Dinucleoside Phosphates / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D057075:Enzyme Assays; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015394:Molecular Structure; D009713:Nucleotidyltransferases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010636:Phenols / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D054852:Small Molecule Libraries / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D001600:Berberine Alkaloids; D015226:Dinucleoside Phosphates; D004791:Enzyme Inhibitors; D010636:Phenols; D054852:Small Molecule Libraries; C528998:cyclic diadenosine phosphate; C000666:coralyne; D009713:Nucleotidyltransferases; C032037:4-bromophenol