title: Structure and biological perspectives of Cu(II)-indomethacin complexes.
abstract: The copper(II) complexes with the non-steroidal anti-inflammatory drug indomethacin (Hindo) in the presence of the nitrogen-donor heterocyclic ligands 2,2'-bipyridine (bipy), 1,10-phenanthroline (phen) or 2,2'-bipyridylamine (bipyam) have been synthesized and characterized. The crystal structures of [Cu(indo)2(bipy)]∙1.5MeOH∙0.5H2O and [Cu(indo)2(phen)] ∙1.85MeOH∙0.15H2O have been determined by X-ray crystallography. All compounds have been tested for their antioxidant and free radical scavenging activity as well as for their in vitro inhibitory activity against soybean lipoxygenase showing significant activity with the previously reported complex [Cu2(indo)4(H2O)2] being the most active. The complexes exhibit good binding affinity to human or bovine serum albumin protein with high binding constant values. UV study of the interaction of the complexes with calf-thymus (CT) DNA has shown that the complexes can bind to CT DNA with [Cu(indo)2(bipyam)] showing the highest binding constant to CT DNA (Kb=1.56(±0.19)×10(6)M(-1)). The complexes can bind to CT DNA via intercalation as concluded by cyclic voltammetry, DNA viscosity measurements and competitive studies with ethidium bromide (EB) which revealed the ability of the complexes to displace the DNA-bound EB.
authors.affiliation: Department of General and Inorganic Chemistry, Faculty of Chemistry, Aristotle University of Thessaloniki, P.O. Box 135, GR-54124 Thessaloniki, Greece.
authors.forename: Alketa
authors.initials: A
authors.lastname: Tarushi
authors.affiliation: Institute of Advanced Materials, Physicochemical Processes, Nanotechnology & Microsystems, Department of Materials Science, NCSR "Demokritos", GR-15310 Aghia Paraskevi Attikis, Greece.
authors.forename: Catherine P
authors.initials: CP
authors.lastname: Raptopoulou
authors.affiliation: Institute of Advanced Materials, Physicochemical Processes, Nanotechnology & Microsystems, Department of Materials Science, NCSR "Demokritos", GR-15310 Aghia Paraskevi Attikis, Greece.
authors.forename: Vassilis
authors.initials: V
authors.lastname: Psycharis
authors.affiliation: Department of General and Inorganic Chemistry, Faculty of Chemistry, Aristotle University of Thessaloniki, P.O. Box 135, GR-54124 Thessaloniki, Greece.
authors.forename: Dimitris P
authors.initials: DP
authors.lastname: Kessissoglou
authors.affiliation: Department of Nutrition and Dietetics, Faculty of Food Technology and Nutrition, Alexandrion Technological Educational Institution, Sindos, Thessaloniki, Greece. Electronic address: papadnas@nutr.teithe.gr.
authors.forename: Athanasios N
authors.initials: AN
authors.lastname: Papadopoulos
authors.affiliation: Department of General and Inorganic Chemistry, Faculty of Chemistry, Aristotle University of Thessaloniki, P.O. Box 135, GR-54124 Thessaloniki, Greece. Electronic address: gepsomas@chem.auth.gr.
authors.forename: George
authors.initials: G
authors.lastname: Psomas
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002417:Cattle; D003300:Copper / Q000737:chemistry* / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004247:DNA / Q000187:drug effects; D007213:Indomethacin / Q000737:chemistry* / Q000494:pharmacology; D015394:Molecular Structure; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000975:Antioxidants; D003300:Copper; D004247:DNA; D007213:Indomethacin
keywords: Antioxidant activity; Copper complexes; Indomethacin; Interaction with DNA; Interaction with albumins