title: Ileal interposition reduces blood glucose levels and decreases insulin resistance in a type 2 diabetes mellitus animal model by up-regulating glucagon-like peptide-1 and its receptor.
abstract: This study is to explore the possible mechanism of ileal interposition (IT) treatment of glycemic control of the type 2 diabetes mellitus (T2DM) by establishing an IT animal model. Twelve T2DM rats (GK rats) of 8-week old were divided into GK IT surgery group (GK-IT) and GK sham group (GK-Sham). Six Wistar rats were used as the non-T2DM sham group (WS-Sham). Enzyme-linked immunosorbent assay was used to detect plasma insulin concentration and fasting pancreas glucagon-like peptide-1 (GLP-1) concentration changes. Homeostasis model assessment of insulin resistance was used to quantitatively measure insulin resistance. Glucagon-like peptide-1 receptor (GLP-1R) expression was detected by Western blotting. IT significantly decreased fasting blood glucose level and the oral glucose tolerance, and reduced insulin resistance of GK rats by increasing GLP-1 concentration and GLP-1R levels. The postoperative pancreatic β-cell apoptosis rate of GK-Sham group was significantly higher than those in the GK-IT group and the WS-Sham group. IT significantly reduces blood glucose and decreases insulin resistance by up-regulating GLP-1 concentrations and GLP-1R levels, which may contribute to insulin secretion of pancreatic β-cells and decreases apoptosis of pancreatic β-cell.
authors.affiliation: Department of Gastrointestinal Tumor Surgery, Shandong Cancer Hospital 440 Jiyan Road, Jinan 250117, Shandong, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Sun
authors.affiliation: Breast Cancer Center, Shandong Cancer Hospital 440 Jiyan Road, Jinan 250117, Shandong, China.
authors.forename: Meizhu
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University Beijing 100050, China.
authors.forename: Maomin
authors.initials: M
authors.lastname: Song
authors.affiliation: Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University Beijing 100050, China.
authors.forename: Rixing
authors.initials: R
authors.lastname: Bai
authors.affiliation: Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University Beijing 100050, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Cheng
authors.affiliation: Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University Beijing 100050, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xing
authors.affiliation: Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University Beijing 100050, China.
authors.forename: Huisheng
authors.initials: H
authors.lastname: Yuan
authors.affiliation: Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University Beijing 100050, China.
authors.forename: Pilin
authors.initials: P
authors.lastname: Wang
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000502:physiology; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000601:surgery*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism / Q000601:surgery*; D004797:Enzyme-Linked Immunosorbent Assay; D052216:Glucagon-Like Peptide 1 / Q000096:biosynthesis*; D000067757:Glucagon-Like Peptide-1 Receptor; D007082:Ileum; D007333:Insulin Resistance / Q000502:physiology; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D007581:Jejunoileal Bypass; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018027:Receptors, Glucagon / Q000096:biosynthesis*; D015854:Up-Regulation
chemical_list: D001786:Blood Glucose; C000593835:Glp1r protein, rat; D000067757:Glucagon-Like Peptide-1 Receptor; D018027:Receptors, Glucagon; D052216:Glucagon-Like Peptide 1
keywords: Ileal interposition; glucagon-like peptide-1 (GLP-1); glucagon-like peptide-1 receptor (GLP-1R); type 2 diabetes mellitus (T2DM)