title: Identification of a telomeric DNA-binding protein in Eimeria tenella.
abstract: Coccidiosis is considered to be a major problem for the poultry industry, and coccidiosis control is yet urgent. Due to the roles in telomere length regulation and end protection, telomere-binding proteins have been considered as a good target for drug design. In this work, a putative Gbp1p that is similar to telomeric DNA-binding protein Gbp (G-strand binding protein) of Cryptosporidium parvum, was searched in the database of Eimeria tenella. Sequence analysis indicated E.tenella Gbp1p (EtGbp1p) has significant sequence similarity to other eukaryotic Gbps in their RNA recognition motif (RRM) domains. Electrophoretic mobility shift assays (EMSAs) demonstrated recombinant EtGbp1p bound G-rich telomeric DNA, but not C-rich or double-stranded telomeric DNA sequences. Competition and antibody supershift assays confirmed the interaction of DNA-protein complex. Chromatin immunoprecipitation assays confirmed that EtGbp1p interacted with telomeric DNA in vivo. Collectively, these evidences suggest that EtGbp1p represents a G-rich single-stranded telomeric DNA-binding protein in E.tenella.
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
authors.forename: Pengtao
authors.initials: P
authors.lastname: Gong
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Life Science, Jilin University, 2699 Qianjin Street, Changchun 130012, China.
authors.forename: Baiqi
authors.initials: B
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China. Electronic address: jianhuali7207@163.com.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
authors.forename: Zhengtao
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
authors.forename: He
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
authors.forename: Ju
authors.initials: J
authors.lastname: Yang
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
authors.forename: Guocai
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China. Electronic address: xczhang@jlu.edu.cn.
authors.forename: Xichen
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D016786:Eimeria tenella / Q000235:genetics*; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D016415:Sequence Alignment; D034501:Telomere-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D015800:Protozoan Proteins; D034501:Telomere-Binding Proteins
keywords: Eimeria tenella; EtGbp1p; RRM; Telomeric DNA-binding protein