title: The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells.
abstract: Enamel matrix derivative (EMD) is widely used in periodontal tissue regeneration therapy. However, because the bioactivity of EMD varies from batch to batch, and the use of a synthetic peptide could avoid use from an animal source, a completely synthetic peptide (SP) containing the active component of EMD would be useful. In this study an oligopeptide synthesized derived from EMD was evaluated for whether it contributes to periodontal tissue regeneration. We investigated the effects of the SP on cell proliferation and osteoblast differentiation of human mesenchymal stem cells (MSCs), which are involved in tissue regeneration. MSCs were treated with SP (0 to 1000 ng/mL), to determine the optimal concentration. We examined the effects of SP on cell proliferation and osteoblastic differentiation indicators such as alkaline phosphatase activity, the production of procollagen type 1 C-peptide and osteocalcin, and on mineralization. Additionally, we investigated the role of extracellular signal-related kinases (ERK) in cell proliferation and osteoblastic differentiation induced by SP. Our results suggest that SP promotes these processes in human MSCs, and that ERK inhibitors suppress these effects. In conclusion, SP promotes cell proliferation and osteoblastic differentiation of human MSCs, probably through the ERK pathway.
authors.affiliation: Department of Periodontology, Osaka Dental University, Osaka 573-1121, Japan. katayama@cc.osaka-dent.ac.jp.
authors.forename: Nobuhito
authors.initials: N
authors.lastname: Katayama
authors.affiliation: Department of Oral Pathology, Osaka Dental University, Osaka 573-1121, Japan. kato-h@cc.osaka-dent.ac.jp.
authors.forename: Hirohito
authors.initials: H
authors.lastname: Kato
authors.affiliation: Department of Periodontology, Osaka Dental University, Osaka 573-1121, Japan. taguchi@cc.osaka-dent.ac.jp.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Taguchi
authors.affiliation: Department of Oral Pathology, Osaka Dental University, Osaka 573-1121, Japan. tanaka@cc.osaka-dent.ac.jp.
authors.forename: Akio
authors.initials: A
authors.lastname: Tanaka
authors.affiliation: Department of Periodontology, Osaka Dental University, Osaka 573-1121, Japan. umeda-m@cc.osaka-dent.ac.jp.
authors.forename: Makoto
authors.initials: M
authors.lastname: Umeda
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D003743:Dental Enamel / Q000737:chemistry*; D005419:Flavonoids / Q000494:pharmacology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects*; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D015675:Osteocalcin / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D005419:Flavonoids; D009842:Oligopeptides; D015675:Osteocalcin; D000469:Alkaline Phosphatase; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one