title: Anti-Inflammatory Effect of Mangostenone F in Lipopolysaccharide-Stimulated RAW264.7 Macrophages by Suppressing NF-κB and MAPK Activation.
abstract: Mangostenone F (MF) is a natural xanthone isolated from Garcinia mangostana. However, little is known about the biological activities of MF. This study was designed to investigate the anti-inflammatory effect and underlying molecular mechanisms of MF in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. MF dose-dependently inhibited the production of NO, iNOS, and pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β) in LPS-stimulated RAW264.7 macrophages. Moreover, MF decreased the NF-κB luciferase activity and NF-κB DNA binding capacity in LPS-stimulated RAW264.7 macrophages. Furthermore, MF suppressed the NF-κB activation by inhibiting the degradation of IκBα and nuclear translocation of p65 subunit of NF-κB. In addition, MF attenuated the AP-1 luciferase activity and phosphorylation of ERK, JNK, and p38 MAP kinases. Taken together, these results suggest that the anti-inflammatory effect of MF is associated with the suppression of NO production and iNOS expression through the down-regulation of NF-κB activation and MAPK signaling pathway in LPS-stimulated RAW264.7 macrophages.
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185.
authors.forename: Byoung Ok
authors.initials: BO
authors.lastname: Cho
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon-gun 363-883.
authors.forename: Hyung Won
authors.initials: HW
authors.lastname: Ryu
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185.
authors.forename: Yangkang
authors.initials: Y
authors.lastname: So
authors.affiliation: Department of Food Science and Human Nutrition, Chungnam National University, Daejeon 305-764.
authors.forename: Chang Wook
authors.initials: CW
authors.lastname: Lee
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185.
authors.forename: Chang Hyun
authors.initials: CH
authors.lastname: Jin
authors.affiliation: Department of Food Science and Human Nutrition, Chungnam National University, Daejeon 305-764.
authors.forename: Hong Sun
authors.initials: HS
authors.lastname: Yook
authors.affiliation: Department of Microbiology, College of Medicine, Seonam University, Namwon 590-711, Republic of Korea.
authors.forename: Yong Wook
authors.initials: YW
authors.lastname: Jeong
authors.affiliation: Department of Microbiology, College of Medicine, Seonam University, Namwon 590-711, Republic of Korea.
authors.forename: Jong Chun
authors.initials: JC
authors.lastname: Park
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185.
authors.forename: Il Yun
authors.initials: IY
authors.lastname: Jeong
keywords: MAPK; Mangostenone F; NF-κB; NO; iNOS