title: Regulating drug release from pH- and temperature-responsive electrospun CTS-g-PNIPAAm/poly(ethylene oxide) hydrogel nanofibers.
abstract: Temperature- and pH-responsive polymers have been widely investigated as smart drug release systems. However, dual-sensitive polymers in the form of nanofibers, which is advantageous in achieving rapid transfer of stimulus to the smart polymeric structures for regulating drug release behavior, have rarely been explored. In this study, chitosan-graft-poly(N-isopropylacrylamide) (CTS-g-PNIPAAm) copolymer was synthesized by using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxy succinimide (NHS) as grafting agents to graft carboxyl-terminated PNIPAAm (PNIPAAm-COOH) chains onto the CTS biomacromolecules, and then CTS-g-PNIPAAm with or without bovine serum albumin (BSA) was fabricated into nanofibers through electrospinning using poly(ethylene oxide) (PEO, 10 wt%) as a fiber-forming facilitating additive. The BSA laden CTS-g-PNIPAAm/PEO hydrogel nanofibers were tested to determine their drug release profiles by varying pH and temperature. Finally, cytotoxicity of the CTS-g-PNIPAAm/PEO hydrogel nanofibers was evaluated by assaying the L929 cell proliferation using the MTT method. It was found that the synthesized CTS-g-PNIPAAm possessed a temperature-induced phase transition and lower critical solution temperature (LCST) at 32° C in aqueous solutions. The rate of BSA release could be well modulated by altering the environmental pH and temperature of the hydrogel nanofibers. The CTS-g-PNIPAAm/PEO hydrogel nanofibers supported L929 cell growth, indicative of appropriate cytocompatibility. Our current work could pave the way towards developing multi-stimuli responsive nanofibrous smart materials for potential applications in the fields of drug delivery and tissue engineering.
authors.affiliation: College of Chemistry, Chemical Engineering & Biotechnology, Donghua University, Shanghai 201620, People's Republic of China. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai 201620, People's Republic of China.
authors.forename: Huihua
authors.initials: H
authors.lastname: Yuan
authors.forename: Biyun
authors.initials: B
authors.lastname: Li
authors.forename: Kai
authors.initials: K
authors.lastname: Liang
authors.forename: Xiangxin
authors.initials: X
authors.lastname: Lou
authors.forename: Yanzhong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry* / Q000633:toxicity; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002417:Cattle; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry / Q000633:toxicity; D016503:Drug Delivery Systems; D055664:Electrochemical Techniques; D020100:Hydrogels; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D057139:Nanofibers / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000633:toxicity; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000493:pharmacokinetics; D013696:Temperature; D017155:Wettability
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D020100:Hydrogels; C508427:PEG-graft-trimethyl-chitosan copolymer; C052970:poly-N-isopropylacrylamide; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D048271:Chitosan