title: Improvement of the fermentative activity of lactic acid bacteria starter culture by the addition of Mn²⁺.
abstract: Production of lactic acid bacteria (LAB) starter with raw material has received much scientific investigation, but little information is available on the influences of some trace elements on the growth and fermentative activity of LAB. Based on this fact, this paper aimed to investigate the effects of Mn(2+) on the performance of Lactobacillus plantarum CX-15 starter with Jerusalem artichoke (JA) as the main medium substrate. The results showed that Mn(2+) addition had a significant beneficial affect on the fermentative activity of L. plantarum CX-15 starter. In contrast, the lack of Mn(2+) would cause the subsequent fermentation significantly slower, whether the cell density in starter culture was higher or lower. The possible mechanism of these phenomenons was further elucidated by the time course analysis of the specific activities of metabolism key enzymes during the culture processes of L. plantarum CX-15 starter. Compared to the fermentation processes without Mn(2+) addition, it was found that Mn(2+) addition would enhance the lactate dehydrogenase (LDH) activity but reduce the activities of pyruvate dehydrogenase (PDH) and ATPase activity. Therefore, it could be concluded that the improvement of L. plantarum starter fermentative activity was probably a consequence of Mn(2+) acting as "metabolic switch," which regulated the metabolic flux from pyruvic acid to lactic acid and other metabolism pathway.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Cheng
authors.forename: Ying
authors.initials: Y
authors.lastname: Dong
authors.forename: Ping
authors.initials: P
authors.lastname: Su
authors.forename: Xiang
authors.initials: X
authors.lastname: Xiao
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000379:methods; D006368:Helianthus / Q000382:microbiology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D019344:Lactic Acid / Q000096:biosynthesis*; D048191:Lactiplantibacillus plantarum / Q000187:drug effects / Q000378:metabolism*; D008345:Manganese / Q000494:pharmacology*; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism*
chemical_list: D011768:Pyruvate Dehydrogenase Complex; D019344:Lactic Acid; D008345:Manganese; D007770:L-Lactate Dehydrogenase; D000251:Adenosine Triphosphatases