title: Evaluation of in vitro enzymatic and non-enzymatic antioxidant properites of leaf extract from Alpinia Purpurata (Vieill.) K. Schum.
abstract: OBJECTIVE
  To evaluate the enzymatic and non-enzymatic antioxidants of leaf extract from Alpinia purpurata.
  
  
  METHODS
  One gram of fresh leaf of Alpinia purpurata was grinded in 2 mL of 50% ethanol and centrifuged at 10,000×g at 4°C for 10 min. The supernatant obtained was used within 4 h for various enzymatic antioxidants assays like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), ascorbate oxidase, peroxidase, polyphenol oxidase (PPO) and non-enzymatic antioxidants such as vitamin C, total reduced glutathione (TRG) and lipid peroxidation (LPO).
  
  
  RESULTS
  The leaf extract of Alpinia purpurata possess antioxidants like vitamin C 472.92±6.80 μg/mg protein, GST 372.11±5.70 μmol of 1-chloro 2,4 dinitrobenzene (CDNB)-reduced glutathione (GSH) conjugate formed/min/mg protein, GPx 281.69±6.43 μg of glutathione oxidized/min/mg protein, peroxidases 173.12±9.40 μmol/g tissue, TRG 75.27±3.55 μg/mg protein, SOD 58.03±2.11 U/mg protein, CAT 46.70±2.35 μmol of H2O2 consumed/min/mg protein in high amount whereas ascorbate oxidase 17.41±2.46 U/g tissue, LPO 2.71±0.14 nmol/L of malondialdehyde formed/min/mg protein and PPO 1.14±0.11 μmol/g tissue in moderate amount.
  
  
  CONCLUSIONS
  Alpinia purpurata has the potential to scavenge the free radicals and protect against oxidative stress causing diseases. In future, Alpinia purpurata may serve as a good pharmacotherapeutic agent.
authors.affiliation: Department of Biochemistry, Karpagam University, Coimbatore, Tamil Nadu, India, 641021.
authors.forename: Chinthamony Arul
authors.initials: CA
authors.lastname: Raj
authors.affiliation: Department of Biochemistry, Karpagam University, Coimbatore, Tamil Nadu, India, 641021.
authors.forename: Paramasivam
authors.initials: P
authors.lastname: Ragavendran
authors.affiliation: Department of Biochemistry, Karpagam University, Coimbatore, Tamil Nadu, India, 641021.
authors.forename: Dominic
authors.initials: D
authors.lastname: Sophia
authors.affiliation: Department of Bioinformatics, Karpagam University, Coimbatore, Tamil Nadu, India, 641021.
authors.forename: Thangarajan
authors.initials: T
authors.lastname: Starlin
authors.affiliation: Department of Biochemistry, Sree Narayana Guru College of Arts and Science, KG. Chavadi, Coimbator, Tamil Nadu, 641021, India.
authors.forename: Muthian Ahalliya
authors.initials: MA
authors.lastname: Rathi
authors.affiliation: Department of Biochemistry, Karpagam University, Coimbatore, Tamil Nadu, India, 641021. vkgopalakrishnan@gmail.com.|Department of Bioinformatics, Karpagam University, Coimbatore, Tamil Nadu, India, 641021. vkgopalakrishnan@gmail.com.|Department of Biochemistry, Sree Narayana Guru College of Arts and Science, KG. Chavadi, Coimbator, Tamil Nadu, 641021, India. vkgopalakrishnan@gmail.com.
authors.forename: Velliyur Kanniappan
authors.initials: VK
authors.lastname: Gopalakrishnan
mesh_terms: D032429:Alpinia / Q000737:chemistry*; D000975:Antioxidants / Q000032:analysis*; D004156:Catechol Oxidase / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D015227:Lipid Peroxidation; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D004798:Enzymes; D010936:Plant Extracts; D004156:Catechol Oxidase
keywords: Alpinia purpurata; enzymatic antioxidants; free radicals; non-enzymatic antioxidants; oxidative stress