title: In vivo investigations on luteotropic activity of prostaglandins during early diestrus in nonpregnant bitches.
abstract: The aim of this study was to test for the postulated luteotropic effect of prostaglandin E2 during early diestrus in the dog in an in vivo study. This study was performed on 30 bitches which were randomly assigned to a treatment group (TG) and a control group. Starting on the day of ovulation (Day 0), dogs of the TG were treated for 5, 10, 20, or 30 days with 10 mg firocoxib/kg body weight per day (Previcox, a selective PTGS2 inhibitor) and ovariohysterectomized for collection of corpora lutea on the last day of treatment. Similarly, dogs of the control group were ovariohysterectomized on Days 0, 5, 10, 20, and 30. Blood samples for progesterone measurement were collected every second day; additionally, the area of luteal cell nuclei and the expression of 3β-hydroxysteroid-dehydrogenase at the mRNA and the protein levels were assessed. Mean P4 concentrations were lower in TGs; however, a significant difference was only observed on Day 10. This observation is in line with the finding that treatment with firocoxib reduced expression of 3β-hydroxysteroid-dehydrogenase mRNA and protein (P < 0.05) and the area of luteal cell nuclei (P < 0.05). The results of this study further point to the postulated luteotropic function of prostaglandin E2.
authors.affiliation: Department of Animal Reproduction with Clinic, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland. Electronic address: krz@uwm.edu.pl.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Janowski
authors.affiliation: Clinic for Veterinary Obstetrics, Gynecology and Andrology, Justus-Liebig-University Giessen, Giessen, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Fingerhut
authors.affiliation: Institute of Veterinary Anatomy, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
authors.forename: Mariusz P
authors.initials: MP
authors.lastname: Kowalewski
authors.affiliation: Department of Animal Reproduction with Clinic, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Zduńczyk
authors.affiliation: Department of Animal Reproduction with Clinic, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Domosławska
authors.affiliation: Department of Animal Reproduction with Clinic, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Jurczak
authors.affiliation: Institute of Veterinary Anatomy, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
authors.forename: Alois
authors.initials: A
authors.lastname: Boos
authors.affiliation: Clinic for Veterinary Obstetrics, Gynecology and Andrology, Justus-Liebig-University Giessen, Giessen, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Schuler
authors.affiliation: Clinic for Veterinary Obstetrics, Gynecology and Andrology, Justus-Liebig-University Giessen, Giessen, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Hoffmann
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D015107:4-Butyrolactone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D003338:Corpus Luteum / Q000187:drug effects*; D004285:Dogs / Q000502:physiology*; D030762:Estrous Cycle / Q000502:physiology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D010060:Ovulation; D011374:Progesterone / Q000097:blood; D011448:Prostaglandin Antagonists / Q000008:administration & dosage / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013450:Sulfones / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D011448:Prostaglandin Antagonists; D012333:RNA, Messenger; D013450:Sulfones; D011374:Progesterone; D015096:3-Hydroxysteroid Dehydrogenases; D015107:4-Butyrolactone; C487384:firocoxib
keywords: 3βHSD; Bitch; Corpus luteum; Luteal cell nuclei area; PTGS2 inhibitor; Progesterone