title: Nanovesicles engineered from ES cells for enhanced cell proliferation.
abstract: Extracellular vesicles (Exosomes and microvesicles) have drawn wide attentions in both diagnostic and therapeutic applications, since they are considered to shuttle biological signals intercellularly. However, further research on exosomes is limited by their rarity and heterogeneity even after lengthy isolation processes. In particular, these limitations are challenging in therapeutic applications. To meet these demands, cell-derived nanovesicles that mimic exosomes were generated by extruding living embryonic stem cells through micro-filters. These nanovesicles have an enclosed lipid bilayer and contain cellular contents. The present study investigated the ability of these nanovesicles to improve proliferation by treating primary murine skin fibroblasts with the nanovesicles. The treated skin fibroblasts showed higher expression levels of mRNA, VEGF-α, protein levels of TGF-β collagen I, PCNA, and Ki-67, as well as enhanced cell proliferation rate and number, compared to non-treated cells. The results indicate that treatment with the nanovesicles could potentially contribute to recovery or wound healing process of tissues.
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, POSTECH, 77 Cheongam-Ro, Pohang, Gyeongbuk 790-784, Republic of Korea.
authors.forename: Dayeong
authors.initials: D
authors.lastname: Jeong
authors.affiliation: Department of Mechanical Engineering, POSTECH, 77 Cheongam-Ro, Pohang, Gyeongbuk 790-784, Republic of Korea.
authors.forename: Wonju
authors.initials: W
authors.lastname: Jo
authors.affiliation: Department of Mechanical Engineering, POSTECH, 77 Cheongam-Ro, Pohang, Gyeongbuk 790-784, Republic of Korea.
authors.forename: Jaewoong
authors.initials: J
authors.lastname: Yoon
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, POSTECH, 77 Cheongam-Ro, Pohang, Gyeongbuk 790-784, Republic of Korea.
authors.forename: Junho
authors.initials: J
authors.lastname: Kim
authors.affiliation: School of Kinesiology, 1402 Washington Heights, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Sachi
authors.initials: S
authors.lastname: Gianchandani
authors.affiliation: Department of Life Science, POSTECH, 77 Cheongam-Ro, Pohang, Gyeongbuk 790-784, Republic of Korea.
authors.forename: Yong Song
authors.initials: YS
authors.lastname: Gho
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, POSTECH, 77 Cheongam-Ro, Pohang, Gyeongbuk 790-784, Republic of Korea; Department of Mechanical Engineering, POSTECH, 77 Cheongam-Ro, Pohang, Gyeongbuk 790-784, Republic of Korea. Electronic address: jpark@postech.ac.kr.
authors.forename: Jaesung
authors.initials: J
authors.lastname: Park
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology*; D055354:Exosomes / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism; D014945:Wound Healing
chemical_list: D002352:Carrier Proteins; D024042:Collagen Type I; C561966:PAF protein, mouse; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse
keywords: Cell proliferation; ECM; Fibroblasts; Growth factor; Nanoparticles; Stem cell