title: Kinetics of mushroom tyrosinase and melanogenesis inhibition by N-acetyl-pentapeptides.
abstract: We investigated the kinetics of 4N-acetyl-pentapeptides, Ac-P1, Ac-P2, Ac-P3, and Ac-P4, regarding inhibition of mushroom tyrosinase activity. The peptides sequences of Ac-P1, Ac-P2, Ac-P3, and Ac-P4 were Ac-RSRFK, Ac-KSRFR, Ac-KSSFR, and Ac-RSRFS, respectively. The 4N-acetyl-pentapeptides were able to reduce the oxidation of l-DOPA by tyrosinase in a dose-dependent manner. Of the 4N-acetyl-pentapeptides, only Ac-P4 exhibited lag time (80 s) at a concentration of 0.5 mg/mL. The tyrosinase inhibitory effects of Ac-P4 (IC50 0.29 mg/mL) were more effective than those of Ac-P1, Ac-P2, and Ac-P3, in which IC50s were 0.75 mg/mL, 0.78 mg/mL, and 0.81 mg/mL, respectively. Kinetic analysis demonstrated that all 4N-acetyl-pentapeptides were mixed-type tyrosinase inhibitors. Furthermore, 0.1 mg/mL of Ac-P4 exhibited significant melanogenesis inhibition on B16F10 melanoma cells and was more effective than kojic acid. The melanogenesis inhibition of Ac-P4 was dose-dependent and did not induce any cytotoxicity on B16F10 melanoma cells.
authors.affiliation: Department of Chemistry, National Chiayi University, Chiayi 60004, Taiwan.
authors.forename: Ching-Yi
authors.initials: CY
authors.lastname: Lien
authors.affiliation: Department of Applied Cosmetology, Hungkuang University, No. 1018, Sector 6, Taiwan Boulevard, Shalu District, Taichung 43302, Taiwan.
authors.forename: Ching-Yu
authors.initials: CY
authors.lastname: Chen
authors.affiliation: Department of Applied Cosmetology, Hungkuang University, No. 1018, Sector 6, Taiwan Boulevard, Shalu District, Taichung 43302, Taiwan.
authors.forename: Shih-Ting
authors.initials: ST
authors.lastname: Lai
authors.affiliation: Department of Applied Cosmetology, Hungkuang University, No. 1018, Sector 6, Taiwan Boulevard, Shalu District, Taichung 43302, Taiwan.
authors.forename: Chin-Feng
authors.identifier: 0000-0003-1681-8651
authors.initials: CF
authors.lastname: Chan
mesh_terms: D000363:Agaricales / Q000201:enzymology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008545:Melanoma / Q000378:metabolism*; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology*
chemical_list: D009842:Oligopeptides; D014442:Monophenol Monooxygenase