title: Perchlorate-induced oxidative stress in isolated liver mitochondria.
abstract: As a new threat to environment all through the world, perchlorate (ClO4(-)) was predominantly a thyrotoxin, and its toxic manifestations in non-thyroid were also documented. To date, little is known about the effect of ClO4(-) on cell and organelle. To reveal the toxicity of ClO4(-) on living organism in-depth, mitochondria isolated from liver of Carassius auratus were incubated with different concentrations of ClO4(-). The results demonstrated that ClO4(-)-induced mitochondrial oxidative stress, and subsequently caused a gradual opening of permeability transition pore leading to mitochondrial swelling and lipid peroxidative membrane damage. ClO4(-) has a conspicuous inhibition of electron transport chain activity which largely correlated to complexes I and IV. The investigations clearly demonstrated the oxidative stress of ClO4(-) in mitochondria, may well reveal cytotoxic effects in vitro that merit further investigation.
authors.affiliation: Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory, College of Resources and Environmental Sciences, Wuhan University, Wuhan, 430072, Hubei, China, 50654069@qq.com.
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Zhao
authors.forename: Peijiang
authors.initials: P
authors.lastname: Zhou
authors.forename: Xiu
authors.initials: X
authors.lastname: Chen
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Ding
mesh_terms: D000818:Animals; D006054:Goldfish; D008099:Liver / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010472:Perchlorates / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D010472:Perchlorates; D014874:Water Pollutants, Chemical; C494474:perchlorate