title: Bioactive constituents from Croton sparsiflorus Morong.
abstract: Whole plant extracts of Croton sparsiflorus in methanol have shown significant enzyme inhibition and antioxidant activities. Bioassay-guided isolation of chloroform fraction at pH 3 resulted in the identification of crotsparinine (1) and crotsparine (2), while sparsiflorine (3) was purified from the chloroform fraction at pH 9. The structures of the compounds were confirmed through spectral analyses (EI-MS, (1)H and (13)C NMR). The isolated compounds 1-3 exhibited remarkable enzyme inhibition activity with IC50 values 27.01 ± 1.1, 22.26 ± 1.0 and 18.02 ± 1.3 μM in xanthine oxidase and 48.42 ± 1.5, 48.05 ± 1.4 and 7.42 ± 1.0 μM in acetylcholine esterase assays, respectively. These compounds also showed potent radical scavenging and reducing properties in DPPH and FRAP assays, respectively. The present results suggest the validity of the traditional uses of C. sparsiflorus in rheumatism and gout. Furthermore, the isolated noraporphine alkaloids can be useful in the treatment of neurodegenerative diseases.
authors.affiliation: a Research Lab. II, Department of Chemistry , Government College University , Lahore 54000 , Pakistan.
authors.forename: Durre
authors.initials: D
authors.lastname: Shahwar
authors.forename: Naeem
authors.initials: N
authors.lastname: Ahmad
authors.forename: Asma
authors.initials: A
authors.lastname: Yasmeen
authors.forename: Muhammad Akmal
authors.initials: MA
authors.lastname: Khan
authors.forename: Sami
authors.initials: S
authors.lastname: Ullah
authors.forename: Atta-ur
authors.initials: AU
authors.lastname: Rahman
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000302:isolation & purification; D029802:Croton / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors
chemical_list: D000470:Alkaloids; D002800:Cholinesterase Inhibitors; D016166:Free Radical Scavengers; D010936:Plant Extracts; C455068:crotsparine; C000597444:crotsparinine; C000597445:sparsiflorine; D014969:Xanthine Oxidase
keywords: Croton sparsiflorus; enzyme inhibition; noraporphine alkaloids