title: Instructions for producing a mouse model of glucocorticoid-induced osteoporosis.
abstract: Glucocorticoids are effective drugs used for the treatment of inflammatory diseases such as rheumatoid arthritis or asthma. Furthermore, they regulate various physiological processes, including bone remodeling. However, long-term high- and even low-dose glucocorticoid use is associated with a compromised bone quality and an increased fracture risk. At the cellular level, glucocorticoids suppress bone formation and stimulate bone resorption, which leads to loss of bone mass. To investigate the underlying mechanisms and new therapeutic strategies, the in vivo model for glucocorticoid-induced bone loss is widely used. This protocol outlines the common procedure that is currently used for the induction of bone loss in mice using glucocorticoids. It further provides useful hints and highlights possible pitfalls to take into account before starting an experiment.
authors.affiliation: Division of Endocrinology and Metabolic Bone Diseases, Department of Medicine III, Technical University Dresden , Dresden, Germany.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Thiele
authors.affiliation: Division of Endocrinology and Metabolic Bone Diseases, Department of Medicine III, Technical University Dresden , Dresden, Germany.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Baschant
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Southern Denmark , Odense, Denmark.
authors.forename: Alexander
authors.initials: A
authors.lastname: Rauch
authors.affiliation: Division of Endocrinology and Metabolic Bone Diseases, Department of Medicine III, Technical University Dresden , Dresden, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Rauner