title: IκB kinase epsilon expression in adipocytes is upregulated by interaction with macrophages.
abstract: Macrophage infiltration in the adipose tissue, and the interaction with adipocytes, is well documented to be involved in fat inflammation and obesity-associated complications. In this study, we isolated IκB kinase ε (IKKε) as a key adipocyte factor that is potentially affected by interaction with macrophages in adipose tissue in vivo. We showed that IKKε mRNA expression levels in white adipose tissue were increased in both genetic and diet-induced obese mouse. Furthermore, IKKε mRNA expression was decreased by the administration of vitamin B6, an anti-inflammatory vitamin, and that IKKε expression levels in adipose tissue were closely correlated with the numbers of infiltrating macrophages. In a co-culture system, we showed that IKKε expression in adipocytes was upregulated by interaction with activated macrophages. This study provides novel insight into IKKε, which is involved in adipose tissue inflammation during the development of obesity.
authors.affiliation: a Department of Molecular and Applied Bioscience , Graduate School of Biosphere Science, Hiroshima University , Higashi-Hiroshima , Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Sanada
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kumoto
authors.forename: Haruna
authors.initials: H
authors.lastname: Suehiro
authors.forename: Takafumi
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Fusanori
authors.initials: F
authors.lastname: Nishimura
authors.forename: Norihisa
authors.initials: N
authors.lastname: Kato
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Yanaka
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D000818:Animals; D002450:Cell Communication; D002452:Cell Count; D051550:I-kappa B Kinase / Q000235:genetics*; D008262:Macrophage Activation; D008264:Macrophages / Q000166:cytology* / Q000276:immunology; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; D051550:I-kappa B Kinase
keywords: IKK epsilon; chronic low-grade inflammation; macrophage; obesity