title: On the possible role of macrofungi in the biogeochemical fate of uranium in polluted forest soils.
abstract: Interactions of macrofungi with U, Th, Pb and Ag were investigated in the former ore mining district of Příbram, Czech Republic. Samples of saprotrophic (34 samples, 24 species) and ectomycorrhizal (38 samples, 26 species) macrofungi were collected from a U-polluted Norway spruce plantation and tailings and analyzed for metal content. In contrast to Ag, which was highly accumulated in fruit-bodies, concentrations of U generally did not exceed 3mg/kg which indicates a very low uptake rate and efficient exclusion of U from macrofungi. In ectomycorrhizal tips (mostly determined to species level by DNA sequencing), U contents were practically identical with those of the non-mycorrhizal fine spruce roots. These findings suggest a very limited role of macrofungi in uptake and biotransformation of U in polluted forest soils. Furthermore, accumulation of U, Th, Pb and Ag in macrofungal fruit-bodies apparently does not depend on total content and chemical fractionation of these metals in soils (tested by the BCR sequential extraction in this study).
authors.affiliation: Institute of Geochemistry, Mineralogy and Mineral Resources, Faculty of Science, Charles University, Albertov 6, CZ-12843 Prague 2, Czech Republic; Nuclear Physics Institute, v.v.i., Academy of Sciences of the Czech Republic, Husinec-Řež 130, CZ-25068 Řež near Prague, Czech Republic.
authors.forename: Jaroslava
authors.initials: J
authors.lastname: Kubrová
authors.affiliation: Institute of Geology, v.v.i., Academy of Sciences of the Czech Republic, Rozvojová 269, CZ-16500 Prague 6, Czech Republic.
authors.forename: Anna
authors.initials: A
authors.lastname: Zigová
authors.affiliation: Nuclear Physics Institute, v.v.i., Academy of Sciences of the Czech Republic, Husinec-Řež 130, CZ-25068 Řež near Prague, Czech Republic.
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Randa
authors.affiliation: Institute of Geology, v.v.i., Academy of Sciences of the Czech Republic, Rozvojová 269, CZ-16500 Prague 6, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Rohovec
authors.affiliation: Institute of Microbiology, v.v.i., Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-142 20 Prague 4, Czech Republic.
authors.forename: Milan
authors.initials: M
authors.lastname: Gryndler
authors.affiliation: Nuclear Physics Institute, v.v.i., Academy of Sciences of the Czech Republic, Husinec-Řež 130, CZ-25068 Řež near Prague, Czech Republic.
authors.forename: Ivana
authors.initials: I
authors.lastname: Krausová
authors.affiliation: 8756 Pender Park Drive, Sidney, BC, V8L 3Z5 Canada.
authors.forename: Colin E
authors.initials: CE
authors.lastname: Dunn
authors.affiliation: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Technická 3, CZ-166 28 Prague 6, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Kotrba
authors.affiliation: Nuclear Physics Institute, v.v.i., Academy of Sciences of the Czech Republic, Husinec-Řež 130, CZ-25068 Řež near Prague, Czech Republic; Institute of Geology, v.v.i., Academy of Sciences of the Czech Republic, Rozvojová 269, CZ-16500 Prague 6, Czech Republic. Electronic address: bore.bor@gmail.com.
authors.forename: Jan
authors.initials: J
authors.lastname: Borovička
mesh_terms: D018153:Czech Republic; D017753:Ecosystem; D038821:Mycorrhizae / Q000737:chemistry / Q000378:metabolism*; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000032:analysis*; D014501:Uranium / Q000032:analysis*
chemical_list: D012987:Soil; D012989:Soil Pollutants; D014501:Uranium
keywords: Bioaccumulation; Ectomycorrhizae; Fungi; Lead; Silver; Soil; Thorium; Uranium