title: Prediction of survival for patients with advanced colorectal cancer using (1) H High-resolution magic angle spinning nuclear MR spectroscopy.
abstract: OBJECTIVE
  To evaluate whether the metabolic profiles of colorectal cancer specimens can be used for prediction of survival.
  
  
  METHODS
  The metabolic profiles of colorectal cancer tissues were determined using the high-resolution magic angle spinning (HR MAS) nuclear magnetic resonance (NMR) technique (16.4 T). HR MAS analysis was performed for 52 tissues taken from patients classified as survivors and nonsurvivors (30). Quantitative analysis was performed for each spectrum. Receiver operating characteristic (ROC) curves were used to evaluate the potential to predict patient survival over 5.5 years.
  
  
  RESULTS
  Analysis of (1) H NMR spectra led to the identification and quantitative analysis of 30 metabolites. A significant increase in the Tau/Gly and Tau/MI ratios were associated with long-term survival (P = 0.004 and P = 0.003, respectively). ROC analysis indicated that the Tau/MI ratio had the best predictive value for survival (sensitivity 64.7% and specificity 100%). Good predictive value of survival was found for Tau/Gly ratio (sensitivity 63.6% and specificity 96.3%). Moreover, the Glu/Gln metabolic ratio with a cutoff level of 1.74 was predictive of survival with a sensitivity of 83.3% and a specificity of 85.7%.
  
  
  CONCLUSIONS
  Our results indicate that HR MAS spectroscopy is potentially useful for survival prediction in advanced colorectal cancer.
authors.affiliation: Institute of Organic Chemistry, Faculty of Chemistry, Lodz, Lodz University of Technology, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Pacholczyk-Sienicka
authors.affiliation: Institute of Organic Chemistry, Faculty of Chemistry, Lodz, Lodz University of Technology, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Fabiańska
authors.affiliation: Department of Pathology, Chair of Oncology, Lodz, Medical University of Lodz, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Pasz-Walczak
authors.affiliation: Department of Pathology, Chair of Oncology, Lodz, Medical University of Lodz, Poland.
authors.forename: Radzisław
authors.initials: R
authors.lastname: Kordek
authors.affiliation: Institute of Organic Chemistry, Faculty of Chemistry, Lodz, Lodz University of Technology, Poland.
authors.forename: Stefan
authors.initials: S
authors.lastname: Jankowski
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000401:mortality* / Q000473:pathology; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D015996:Survival Rate
keywords: HR MAS spectroscopy; colorectal cancer; metabolic profile; prediction of survival