title: Clinical characteristics, tumor, node, metastasis status, and mutation rate in domain of epidermal growth factor receptor gene in serbian patients with lung adenocarcinoma.
abstract: OBJECTIVE
  Mutation rate in domain of EGFR gene varies between populations of lung cancer patients. Primary aim of this study was to analyze clinical and pathological characteristics, and tumor, node, metastasis status and stage of diseases, in relation to mutation status.
  
  
  METHODS
  After histological confirmation of lung adenocarcinoma tissue obtained during bronchoscopy was consecutively sent for EGFR testing. Genomic DNA extraction was performed with the QIAamp DNA FFPE Tissue kit. Clinical data for multivariate analysis were extracted from hospital based-lung cancer registry.
  
  
  RESULTS
  Among 360 tested patients, there was 67.8% males and 32.2% females, aged 61 ± 9.8 years. Majority of patients were smokers (57.0%) with Eastern Cooperative Oncology Group 1 performance status (92.2%). Mutation in EGFR gene was detected in 42 (11.7%) patients. Deletion in exon 19 was detected in 24 (6.7%) patients, mutation in exon 21 in 17 (4.7%), and mutation in exon 18 in one patient (0.3%). Patients were mostly diagnosed in stage IV adenocarcinoma (74.4%). Statistically significant differences were determined in relation to smoking (p < 0.001), T descriptor (size; p = 0.019) and gender (p = 0.002).
  
  
  CONCLUSIONS
  Mutation rate in domain of EGFR gene in investigated lung cancer population is in range with reported data in Caucasian race. Smoking, T descriptor and gender were found to be related to the EGFR status.
authors.affiliation: *Institute for Pulmonary Diseases of Vojvodina, Clinic for Pulmonary Oncology, Faculty of Medicine, University of Novi Sad, Sremska Kamenica; †Institute for Oncology and Radiology of Serbia, Belgrade, Serbia; ‡Pulmonary Department-Oncology Unit, "G. Papanikolaou" General Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece; and §ROCHE, Beograd, Serbia.
authors.forename: Bojan
authors.initials: B
authors.lastname: Zaric
authors.forename: Vladimir
authors.initials: V
authors.lastname: Stojsic
authors.forename: Tomi
authors.initials: T
authors.lastname: Kovacevic
authors.forename: Tatjana
authors.initials: T
authors.lastname: Sarcev
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Tepavac
authors.forename: Radmila
authors.initials: R
authors.lastname: Jankovic
authors.forename: Jelena
authors.initials: J
authors.lastname: Spasic
authors.forename: Davorin
authors.initials: D
authors.lastname: Radosavljevic
authors.forename: Paul
authors.initials: P
authors.lastname: Zarogoulidis
authors.forename: Nevena
authors.initials: N
authors.lastname: Vukobradovic-Djoric
authors.forename: Branislav
authors.initials: B
authors.lastname: Perin
mesh_terms: D000230:Adenocarcinoma / Q000453:epidemiology / Q000235:genetics* / Q000556:secondary; D000077192:Adenocarcinoma of Lung; D000328:Adult; D000368:Aged; D001999:Bronchoscopy; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000235:genetics*; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008175:Lung Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000556:secondary; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D059645:Mutation Rate; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D055771:Serbia / Q000453:epidemiology
chemical_list: D004273:DNA, Neoplasm; D066246:ErbB Receptors