title: 17β-Estradiol administration promotes delayed cutaneous wound healing in 40-week ovariectomised female mice.
abstract: This study investigated the effect of 17β-estradiol on wound healing in 40-week ovariectomised female mice. Thirty-six-week-old female mice were divided into three groups: medication with 17β-estradiol after ovariectomy (OVX + 17β-estradiol), ovariectomy (OVX) and sham (SHAM). The mice received two full-thickness wounds, and the OVX + 17β-estradiol group was administered 17β-estradiol at 0·01 g/day until healing. In the OVX + 17β-estradiol group, the ratio of wound area was significantly smaller than those of the OVX and SHAM groups on days 1-3, 5, 6, 8-12 and 9-12, respectively, the numbers of neutrophils and macrophages were significantly smaller than those on days 3 and 7, the ratio of re-epithelialisation was significantly higher than those on days 3 and 11, the ratio of myofibroblasts was significantly higher than those on day 11 and smaller on day 14, and the ratio of collagen fibres was significantly larger than that of the OVX group on days 7-14. We found that 17β-estradiol administration promotes cutaneous wound healing in 40-week female mice by reducing wound area, shortening inflammatory response, and promoting re-epithelialisation, collagen deposition and wound contraction. Our results suggest that cutaneous wound healing that is delayed because of ageing is promoted by exogenous and continuous 17β-estradiol administration.
authors.affiliation: Department of Clinical Nursing, Graduate Course of Nursing Science, Division of Health Sciences, Graduate School of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
authors.forename: Kanae
authors.initials: K
authors.lastname: Mukai
authors.affiliation: Department of Clinical Nursing, Graduate Course of Nursing Science, Division of Health Sciences, Graduate School of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Nakajima
authors.affiliation: Department of Clinical Nursing, Graduate Course of Nursing Science, Division of Health Sciences, Graduate School of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
authors.forename: Tamae
authors.initials: T
authors.lastname: Urai
authors.affiliation: Department of Clinical Nursing, Graduate Course of Nursing Science, Division of Health Sciences, Graduate School of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
authors.forename: Emi
authors.initials: E
authors.lastname: Komatsu
authors.affiliation: Department of Clinical Nursing, Graduate Course of Nursing Science, Division of Health Sciences, Graduate School of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
authors.lastname: Nasruddin
authors.affiliation: Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Sugama
authors.affiliation: Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan. nakatosi@staff.kanazawa-u.ac.jp.
authors.forename: Toshio
authors.initials: T
authors.lastname: Nakatani
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D004958:Estradiol; D005260:Female; D051379:Mice; D010052:Ovariectomy; D012867:Skin; D014945:Wound Healing
chemical_list: D004958:Estradiol
keywords: 17β-Estradiol; Ageing; Cutaneous wound healing; Female mice; Ovariectomy