title: Genetic expression profile of human liver grafts in ischemia-reperfusion injury: comparison of familial amyloidotic polyneuropathy and deceased-donor liver grafts.
abstract: This study aimed to compare the histologic and molecular gene expression at several surgical times (beginning of harvesting, T0; end of cold ischemia period, T1; and after reperfusion, T2) to characterize the ischemia-reperfusion injury (IRI) in deceased-donor liver grafts harvested from patients with familial amyloidotic polyneuropathy (FAP). For this purpose, 54 patients undergoing liver transplantation were studied and divided into 3 groups: deceased donor to cirrhotic recipient (group 1; n = 27), deceased donor to FAP recipient (group 2; n = 15), and FAP donor to cirrhotic recipient (group 3; n = 12). The main comparison was performed between a histologic score (Suzuki score, adding steatosis and neutrophil infiltration), and molecular gene expression of the following genes: interleukin (IL) 1β, IL-6, E-selectin, Fas-ligand, granzyme B, heme oxygenase 1 (HO1), and nitric oxide synthetase (iNOS2A). We observed less neutrophil infiltration levels in group 3 in sample T0 (P = .0082), which was associated with gene expression of HO1 in the biopsies at T2 (P = .022). In group 3, the molecular expression of genes related to attenuated proinflammatory reaction during IRI, iNOS2A at T0 and HO1 at T2, was detected. We conclude that FAP liver grafts express differently the genes associated with an attenuated proinflammatory reaction, presenting less neutrophil infiltration at harvesting. These findings add more knowledge about the better short-term outcomes in patients receiving this type of liver graft.
authors.affiliation: Centro Hepatobiliopancreático e de Transplantação, Universidade Nova de Lisboa, Lisbon, Portugal. Electronic address: fusilis@gmail.com.
authors.forename: J
authors.initials: J
authors.lastname: Paulino
authors.affiliation: Centro Hepatobiliopancreático e de Transplantação, Universidade Nova de Lisboa, Lisbon, Portugal.
authors.forename: E
authors.initials: E
authors.lastname: Vigia
authors.affiliation: CEDOC, Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Lisbon, Portugal.
authors.forename: P
authors.initials: P
authors.lastname: Marcelino
authors.affiliation: CEDOC, Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Lisbon, Portugal.
authors.forename: O
authors.initials: O
authors.lastname: Abade
authors.affiliation: Instituto Gulbenkian de Ciência, Oeiras, Portugal.
authors.forename: J
authors.initials: J
authors.lastname: Sobral
authors.affiliation: Centro de Histocompatibilidade do Sul, Universidade Nova de Lisboa, Lisbon, Portugal.
authors.forename: D
authors.initials: D
authors.lastname: Ligeiro
authors.affiliation: Serviço de Anatomia Patológica, Hospital Curry Cabral, Universidade Nova de Lisboa, Lisbon, Portugal.
authors.forename: A
authors.initials: A
authors.lastname: Carvalho
authors.affiliation: Departamento de Bioestatística, Universidade Nova de Lisboa, Lisbon, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Alves
authors.affiliation: Departamento de Bioestatística, Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Lisbon, Portugal; Departamento de Bioestatística, Universidade Nova de Lisboa, Lisbon, Portugal.
authors.forename: A L
authors.initials: AL
authors.lastname: Papoila
authors.affiliation: Centro de Histocompatibilidade do Sul, Universidade Nova de Lisboa, Lisbon, Portugal.
authors.forename: H
authors.initials: H
authors.lastname: Trindade
authors.affiliation: Centro Hepatobiliopancreático e de Transplantação, Universidade Nova de Lisboa, Lisbon, Portugal.
authors.forename: E
authors.initials: E
authors.lastname: Barroso
mesh_terms: D000328:Adult; D050377:Cold Ischemia; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D016031:Liver Transplantation; D019520:Living Donors; D008297:Male; D008875:Middle Aged; D011115:Polyneuropathies / Q000601:surgery*; D015427:Reperfusion Injury / Q000235:genetics / Q000378:metabolism* / Q000473:pathology