title: Enhanced bioconversion of ethylene glycol to glycolic acid by a newly isolated Burkholderia sp. EG13.
abstract: Burkholderia sp. EG13 with high ethylene glycol-oxidizing activity was isolated from soil, which could be used for the synthesis of glycolic acid from the oxidation of ethylene glycol. Using the resting cells of Burkholderia sp. EG13 as biocatalysts, the optimum reaction temperature and pH were 30 °C and 6.0, respectively. After 24 h of biotransformation, the yield of glycolic acid from 200 mM ethylene glycol was 98.8 %. Furthermore, an integrated bioprocess for the production of glycolic acid which involved in situ product removal (ISPR) was investigated. Using fed-batch method with ISPR, a total of 793 mM glycolic acid has been accumulated in the reaction mixture after the 4th feed.
authors.affiliation: College of Chemistry and Chemical Engineering, Nanjing University of Technology, Nanjing, 210009, People's Republic of China.|College of Petrochemical Engineering, Changzhou University, Changzhou, 213164, People's Republic of China.
authors.forename: Xiaoxin
authors.initials: X
authors.lastname: Gao
authors.affiliation: College of Chemistry and Chemical Engineering, Nanjing University of Technology, Nanjing, 210009, People's Republic of China. zhengfma@gmail.com.
authors.forename: Zhengfei
authors.initials: Z
authors.lastname: Ma
authors.affiliation: College of Petrochemical Engineering, Changzhou University, Changzhou, 213164, People's Republic of China.
authors.forename: Limin
authors.initials: L
authors.lastname: Yang
authors.affiliation: College of Petrochemical Engineering, Changzhou University, Changzhou, 213164, People's Republic of China.
authors.forename: Jiangquan
authors.initials: J
authors.lastname: Ma
mesh_terms: D019117:Burkholderia / Q000378:metabolism*; D002384:Catalysis; D019855:Ethylene Glycol / Q000378:metabolism*; D006016:Glycolates / Q000378:metabolism*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction
chemical_list: D006016:Glycolates; C031149:glycolic acid; D019855:Ethylene Glycol