title: Programmable nanoscaffolds that control ligand display to a G-protein-coupled receptor in membranes to allow dissection of multivalent effects.
abstract: A programmable ligand display system can be used to dissect the multivalent effects of ligand binding to a membrane receptor. An antagonist of the A2A adenosine receptor, a G-protein-coupled receptor that is a drug target for neurodegenerative conditions, was displayed in 35 different multivalent configurations, and binding to A2A was determined. A theoretical model based on statistical mechanics was developed to interpret the binding data, suggesting the importance of receptor dimers. Using this model, extended multivalent arrangements of ligands were constructed with progressive improvements in binding to A2A. The results highlight the ability to use a highly controllable multivalent approach to determine optimal ligand valency and spacing that can be subsequently optimized for binding to a membrane receptor. Models explaining the multivalent binding data are also presented.
authors.affiliation: Laboratory of Bioorganic Chemistry, NIDDK, ‡Laboratory of Biochemistry and Genetics, NIDDK, and §Laboratory of Cell Biology, CCR, NCI, National Institutes of Health , Bethesda, Maryland 20892, United States.
authors.forename: Andrew V
authors.initials: AV
authors.lastname: Dix
authors.forename: Steven M
authors.initials: SM
authors.lastname: Moss
authors.forename: Khai
authors.initials: K
authors.lastname: Phan
authors.forename: Travis
authors.initials: T
authors.lastname: Hoppe
authors.forename: Silvia
authors.initials: S
authors.lastname: Paoletta
authors.forename: Eszter
authors.initials: E
authors.lastname: Kozma
authors.forename: Zhan-Guo
authors.initials: ZG
authors.lastname: Gao
authors.forename: Stewart R
authors.initials: SR
authors.lastname: Durell
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Jacobson
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Appella
mesh_terms: D058917:Adenosine A2 Receptor Antagonists / Q000737:chemistry / Q000494:pharmacology*; D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000737:chemistry / Q000494:pharmacology*; D011485:Protein Binding; D043705:Receptor, Adenosine A2A / Q000737:chemistry / Q000378:metabolism*
chemical_list: D058917:Adenosine A2 Receptor Antagonists; D008024:Ligands; D020135:Peptide Nucleic Acids; D043705:Receptor, Adenosine A2A; D004247:DNA