title: Microwave assisted extraction-solid phase extraction for high-efficient and rapid analysis of monosaccharides in plants.
abstract: Monosaccharides are the fundamental composition units of saccharides which are a common source of energy for metabolism. An effective and simple method consisting of microwave assisted extraction (MAE), solid phase extraction (SPE) and high performance liquid chromatography-refractive index detector (HPLC-RID) was developed for rapid detection of monosaccharides in plants. The MAE was applied to break down the structure of the plant cells and release the monosaccharides, while the SPE procedure was adopted to purify the extract before analysis. Finally, the HPLC-RID was employed to separate and analyze the monosaccharides with amino column. As a result, the extraction time was reduced to 17 min, which was nearly 85 times faster than soxhlet extraction. The recoveries of arabinose, xylose, fructose and glucose were 85.01%, 87.79%, 103.17%, and 101.24%, with excellent relative standard deviations (RSDs) of 1.94%, 1.13%, 0.60% and 1.67%, respectively. The proposed method was demonstrated to be efficient and time-saving, and had been applied to analyze monosaccharides in tobacco and tea successfully.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China; Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Hai-Fang
authors.initials: HF
authors.lastname: Li
authors.affiliation: Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Yunnan Academy of Tobacco Agricultural Science, Yuxi 653100, China. Electronic address: jinyan1968@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Yunnan Academy of Tobacco Agricultural Science, Yuxi 653100, China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Kong
authors.affiliation: Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China. Electronic address: jmlin@mail.tsinghua.edu.cn.
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Lin
mesh_terms: D001089:Arabinose / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005632:Fructose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D008872:Microwaves; D009005:Monosaccharides / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D013499:Surface Properties; D013662:Tea / Q000737:chemistry; D013696:Temperature; D014026:Nicotiana / Q000737:chemistry; D014994:Xylose / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D009005:Monosaccharides; D013662:Tea; D005632:Fructose; D014994:Xylose; D001089:Arabinose; D005947:Glucose
keywords: High performance liquid chromatography; Microwave assisted extraction; Monosaccharide; Rapid sample pretreatment