title: Rheinheimera arenilitoris sp. nov., isolated from seashore sand.
abstract: A Gram-stain-negative, non-flagellated, aerobic and ovoid or rod-shaped bacterium, designated J-MS1(T), was isolated from seashore sand in the South Sea, South Korea, and subjected to a polyphasic taxonomic study. Strain J-MS1(T) was found to grow optimally at 30 °C and pH 7.0-8.0. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain J-MS1(T) belonged to the genus Rheinheimera, clustering coherently with the type strain of Rheinheimera chironomi and sharing 98.34% sequence similarity. Strain J-MS1(T) exhibited 16S rRNA gene sequence similarity of 94.26-96.98% to the type strains of the other species of the genus Rheinheimera. In the phylogenetic trees based on gyrB sequences, strain J-MS1(T) clustered with the type strain of R. chironomi, with which it shared the highest sequence similarity (86.97%). Strain J-MS1(T) contained Q-8 as the predominant ubiquinone and summed feature 3 (C(16:1)ω7c and/or C(16:1)ω6c), C(16:0) and C(18:1)ω7c as the major fatty acids. The major polar lipids detected in strain J-MS1(T) and in the type strain of R. chironomi were phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content of strain J-MS1(T) was 49.8 mol% and its mean DNA-DNA relatedness value with R. chironomi LMG 23818(T) was 12%. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain J-MS1(T) is separated from recognized species of the genus Rheinheimera. On the basis of the data presented, strain J-MS1(T) is considered to represent a novel species of the genus Rheinheimera, for which the name Rheinheimera arenilitoris sp. nov. is proposed. The type strain is J-MS1(T) ( =KCTC 42112(T) =CECT 8623(T)).
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Sung-Min
authors.initials: SM
authors.lastname: Won
authors.affiliation: Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon, Republic of Korea.|Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002841:Chromatiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012822:Silicon Dioxide; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C483858:phosphatidylethanolamine; D012822:Silicon Dioxide; C030778:ubiquinone 8