title: Monodisperse carboxyl-functionalized poly(ethylene glycol)-coated magnetic poly(glycidyl methacrylate) microspheres: application to the immunocapture of β-amyloid peptides.
abstract: Identification and evaluation of small changes in β-amyloid peptide (Aβ) levels in cerebrospinal fluid is of crucial importance for early detection of Alzheimer's disease. Microfluidic detection methods enable effective preconcentration of Aβ using magnetic microparticles coated with Aβ antibodies. Poly(glycidyl methacrylate) microspheres are coated with α-amino-ω-methoxy-PEG5000 /α-amino-ω-Boc-NH-PEG5000 Boc groups deprotected and NH2 succinylated to introduce carboxyl groups. Capillary electrophoresis with laser-induced fluorescence detection confirms the efficient capture of Aβ 1-40 peptides on the microspheres with immobilized monoclonal anti-Aβ 6E10. The capture specificity is confirmed by comparing Aβ 1-40 levels on the anti-IgG-immobilized particles used as a control.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Heyrovský Sq. 2, 162 06, Prague 6, Czech Republic. horak@imc.cas.cz.
authors.forename: Daniel
authors.initials: D
authors.lastname: Horák
authors.forename: Helena
authors.initials: H
authors.lastname: Hlídková
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hiraoui
authors.forename: Myriam
authors.initials: M
authors.lastname: Taverna
authors.forename: Vladimír
authors.initials: V
authors.lastname: Proks
authors.forename: Eliška
authors.initials: E
authors.lastname: Mázl Chánová
authors.forename: Claire
authors.initials: C
authors.lastname: Smadja
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Kučerová
mesh_terms: D000327:Adsorption; D016229:Amyloid beta-Peptides / Q000302:isolation & purification*; D000818:Animals; D002264:Carboxylic Acids / Q000737:chemistry*; D002417:Cattle; D002846:Chromatography, Affinity; D019075:Electrophoresis, Capillary; D007074:Immunoglobulin G / Q000378:metabolism*; D060328:Magnetic Phenomena; D018625:Microscopy, Atomic Force; D008863:Microspheres; D010316:Particle Size; D010446:Peptide Fragments / Q000302:isolation & purification*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D013818:Thermogravimetry
chemical_list: D016229:Amyloid beta-Peptides; D002264:Carboxylic Acids; D007074:Immunoglobulin G; D010446:Peptide Fragments; D011109:Polymethacrylic Acids; C078592:amyloid beta-protein (1-40); C042535:polyglycidyl methacrylate; D011092:Polyethylene Glycols
keywords: CE-LIF detection; functionalization; magnetic; microspheres; β-amyloid peptides