title: Loss of blast heterogeneity in myelodysplastic syndrome and other chronic myeloid neoplasms.
abstract: OBJECTIVE
  Flow cytometry immunophenotyping has been suggested as an adjunctive technique in the evaluation of myeloid malignancies, especially in the myelodysplastic syndromes. However, its use has been limited due to complexity and cost restraints. The goal of this study is to attempt a simpler approach to flow cytometry immunophenotyping in myeloid neoplasms.
  
  
  METHODS
  We analyzed bone marrow specimens of 45 selected patients and an additional 99 consecutive random patients using a limited antibody panel.
  
  
  RESULTS
  Normal CD34-positive blasts show a characteristic pattern of CD13/HLA-DR expression, with three readily identifiable subpopulations. In contrast, myeloid neoplasms frequently show loss of this heterogeneity.
  
  
  CONCLUSIONS
  Analysis of a limited antibody panel with a focus on CD13/HLA-DR expression provides relatively high specificity and sensitivity for the detection of myeloid neoplasms.
authors.affiliation: From the Department of Laboratory Medicine and Pathology, Mayo Clinic Rochester, Rochester, MN. jevremovic.dragan@mayo.edu.
authors.forename: Dragan
authors.initials: D
authors.lastname: Jevremovic
authors.affiliation: From the Department of Laboratory Medicine and Pathology, Mayo Clinic Rochester, Rochester, MN.
authors.forename: Michael M
authors.initials: MM
authors.lastname: Timm
authors.affiliation: From the Department of Laboratory Medicine and Pathology, Mayo Clinic Rochester, Rochester, MN.
authors.forename: Kaaren K
authors.initials: KK
authors.lastname: Reichard
authors.affiliation: From the Department of Laboratory Medicine and Pathology, Mayo Clinic Rochester, Rochester, MN.
authors.forename: William G
authors.initials: WG
authors.lastname: Morice
authors.affiliation: From the Department of Laboratory Medicine and Pathology, Mayo Clinic Rochester, Rochester, MN.
authors.forename: Curtis A
authors.initials: CA
authors.lastname: Hanson
authors.affiliation: From the Department of Laboratory Medicine and Pathology, Mayo Clinic Rochester, Rochester, MN.
authors.forename: David S
authors.initials: DS
authors.lastname: Viswanatha
authors.affiliation: From the Department of Laboratory Medicine and Pathology, Mayo Clinic Rochester, Rochester, MN.
authors.forename: Matthew T
authors.initials: MT
authors.lastname: Howard
authors.affiliation: From the Department of Laboratory Medicine and Pathology, Mayo Clinic Rochester, Rochester, MN.
authors.forename: Phuong L
authors.initials: PL
authors.lastname: Nguyen
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis; D001853:Bone Marrow / Q000473:pathology*; D001854:Bone Marrow Cells / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D019337:Hematologic Neoplasms / Q000473:pathology*; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000473:pathology*; D009196:Myeloproliferative Disorders / Q000473:pathology*; D055815:Young Adult
chemical_list: D015703:Antigens, CD
keywords: Blasts; Flow cytometry; MDS