title: Immunogenicity of recombinant BCGs expressing predicted antigenic epitopes of bovine viral diarrhea virus E2 gene.
abstract: To develop a vaccine to prevent diseases caused by Mycobacterium tuberculosis and bovine viral diarrhea virus (BVDV) simultaneously, recombinant Bacillus Calmette-Guerin (rBCG) vaccines expressing different regions of the BVDV E2 gene were constructed. Using DNASTAR 6.0 software, potential antigenic epitopes were predicted, and six regions were chosen to generate recombinant plasmids with the pMV361 vector (pMV361-E2-1, pMV361-E2-2, pMV361-E2-3, pMV361-E2-4, pMV361-E2-5 and pMV361-E2-6, respectively). The recombinant plasmids were transformed into BCG, and protein expression was thermally induced at 45 °C. Mice were immunized with 5 × 10(6) CFU/200 µL of each rBCG strain. Compared with other groups, BVDV E2 specific antibody titers were higher in mice immunized with rBCG-E2-6. Ratios and numbers of CD4+, CD8+ and IL-12 expressing spleen lymphocytes of the rBCG-E2-6 group also were higher than those of other groups. Thus, the rBCG-E2-6 vaccine showed the highest immunogenicity of all groups based on the humoral and cellular responses to vaccination.
authors.affiliation: College of Chinese Medicine Material, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Dongxu
authors.initials: D
authors.lastname: Liu
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Lu
authors.affiliation: College of Chinese Medicine Material, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Shi
authors.affiliation: College of Chinese Medicine Material, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Fengyan
authors.initials: F
authors.lastname: Su
authors.affiliation: College of Chinese Medicine Material, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Chinese Medicine Material, Jilin Agricultural University, Changchun 130118, China. Electronic address: durui197107@sina.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Du
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D001500:BCG Vaccine / Q000276:immunology* / Q000627:therapeutic use; D002417:Cattle; D030242:Diarrhea Virus 2, Bovine Viral / Q000276:immunology*; D004195:Disease Models, Animal; D000939:Epitopes / Q000276:immunology*; D005260:Female; D030243:Hemorrhagic Syndrome, Bovine / Q000276:immunology / Q000517:prevention & control*; D007111:Immunity, Cellular / Q000276:immunology; D056724:Immunity, Humoral / Q000276:immunology; D007114:Immunization / Q000379:methods / Q000662:veterinary*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009169:Mycobacterium tuberculosis / Q000276:immunology; D014376:Tuberculosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D014614:Vaccines, Synthetic / Q000276:immunology / Q000627:therapeutic use
chemical_list: D000956:Antigens, Viral; D001500:BCG Vaccine; D000939:Epitopes; D014614:Vaccines, Synthetic
keywords: Antigen site; BVDV; Immunogenicity; Recombinant BCG vaccine