title: Recombinant porcine epidermal growth factor-secreting Lactococcus lactis promotes the growth performance of early-weaned piglets.
abstract: BACKGROUND
  Epidermal growth factor (EGF) is an important growth factor in regulation of cell proliferation, differentiation, survival and apoptosis. Studies showed that food-grade Lactococcus lactis (L. lactis) and NICE expression system have superior performance in exogenous protein expression. This study aimed to construct and express porcine EGF (pEGF), and use L. lactis as vehicle for producing and delivering pEGF. Furthermore, investigating biological activity of pEGF and exploring applications feasibility of combination effects of L. lactis and pEGF on early weaned piglets' production.
  
  
  RESULTS
  A recombinant Lactococcus lactis which produced and secreted pEGF at 1000 ng/ml in culture supernatant was generated. Secreted pEGF was a fully biologically active protein, as demonstrated by its capacity to stimulate L929 mouse fibroblast cell line proliferation in vitro. For in vivo study, forty piglets were randomly allocated to control, antibiotic control, empty vector-expressing L. lactis (LL-EV) and pEGF-secreting L. lactis (LL-pEGF). After 14 d of rearing, final body weight and average daily gain in LL-pEGF were greater (P < 0.05, 8.95 vs. 8.37 kg, 206.1 vs. 157.7 g/day, respectively) than those in control, but no significant differences between LL-pEGF, LL-EV and antibiotic control. Overall period average daily feed intake was higher in LL-pEGF, LL-EV and antibiotic control than in control (P < 0.05, 252.9, 255.6, 250.0, 207.3 g/day, respectively). No significant difference was observed on ADFI/ADG. LL-pEGF increased villous height in the duodenum, jejunum and ileum than in control and LL-EV (P < 0.05). Sucrase in the 3 intestinal segments, aminopeptidase A in the duodenum and Jejunum, aminopeptidase N and dipeptidase IV in the duodenum in LL-pEGF were higher than those in control (P < 0.05). Furthermore, Escherichia coli and Enterococcus counts decreased in the ileum and Lactobacillus increased in the ileum and cecum digesta in LL-pEGF compare with the control (P < 0.05). Lactobacillus increased in the cecum in LL-EV compared with control and antibiotic control (P < 0.05).
  
  
  CONCLUSIONS
  We have generated a recombinant Lactococcus lactis which produced and secreted fully biologically active porcine EGF. Oral administration of pEGF-secreting L. lactis had beneficial effects on intestinal health and performance of early-weaned piglets.
authors.forename: Dingyue
authors.initials: D
authors.lastname: Wang
authors.forename: Shengyu
authors.initials: S
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Lin
authors.forename: Zhengfeng
authors.initials: Z
authors.lastname: Fang
authors.forename: Lianqiang
authors.initials: L
authors.lastname: Che
authors.forename: Bai
authors.initials: B
authors.lastname: Xue
authors.affiliation: Institute of Animal Nutrition, Key Laboratory for Animal Disease Resistance Nutrition of Sichuan Province, Sichuan Agricultural University, Ya'an 625014Sichuan, China. pig2pig@sina.com.cn.
authors.forename: De
authors.initials: D
authors.lastname: Wu
mesh_terms: D000821:Animal Feed; D000818:Animals; D002460:Cell Line; D004032:Diet / Q000662:veterinary; D004815:Epidermal Growth Factor / Q000378:metabolism* / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D011994:Recombinant Proteins; D013552:Swine / Q000254:growth & development*; D014886:Weaning
chemical_list: D011994:Recombinant Proteins; D004815:Epidermal Growth Factor