title: [Anti-leukemia mechanism of miR-17 and miR-20a silencing mediated by miRNA sponge].
abstract: This study was aimed to quantitatively detect the expression levels of pre-miR-17 and pre-miR-20a in acute leukemia patients and eight kinds of leukemia cell lines, and to investigate the anti-leukemia mechanism of miR-17 and miR-20a silence mediated by miRNA Sponge. Quantitative real-time PCR was used to detect the mRNA expression levels of pre-miR-17 and pre-miR-20a in patients with various types of leukemia and leukemia cell lines. The Jurkat cells over-expressing miR-17 and miR-20a were transfected with recombinant lentivirus-transfecting units targeted at miR-17 and miR-20a plus 6 µg/ml of polybrene. Then the proliferation ability and cell cycle of Jurkat cells was evaluated by CCK-8 and flow cytometry respectively. The results showed that the expression level of pre-miR-17 and pre-miR-20a in all leukemia patients was significantly higher than that in normal group(P < 0.05), the expression of pre-miR-17 and pre-miR-20a in acute lymphoid leukemia was significantly higher than that in acute myeloid leukemia(P < 0.05), and the pre-miR-17 and pre-miR-20a expression level did not correlate significantly with high white blood cell count>20.0×10(9)/L(P > 0.05). The miR-17 and miR-20a silencing mediated by miRNA Sponge led to a significant decrease of cell growth, restored G1 accumulation and increase of cell apoptosis. It is concluded that the expression of miR-17 and miR-20a is upregulated in leukemia patients, which may contribute to leukemogenesis. Over-expressed miR-17 and miR-20a promote cell growth and cell cycle progression, and inhibit apoptosis through negatively-regulating P21 and E2F1 after-transcriptionally.
authors.affiliation: Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital; Fuzhou 350001, Fujian Province, China.
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Niu
authors.affiliation: Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital; Fuzhou 350001, Fujian Province, China.
authors.forename: Shun-Quan
authors.initials: SQ
authors.lastname: Wu
authors.affiliation: Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital; Fuzhou 350001, Fujian Province, China.
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Xu
authors.affiliation: Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital; Fuzhou 350001, Fujian Province, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
authors.affiliation: Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital; Fuzhou 350001, Fujian Province, China. E-mail: deanzhanrong@yahoo.com.cn.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhan
mesh_terms: D045744:Cell Line, Tumor; D020868:Gene Silencing; D005822:Genetic Vectors; D006801:Humans; D007938:Leukemia / Q000235:genetics* / Q000473:pathology*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*
chemical_list: C524941:MIRN17 microRNA, human; C534451:MIRN20a microRNA, human; D035683:MicroRNAs