title: Trans-species complementation analysis to study function conservation of plant Endosomal Sorting Complex Required for Transport (ESCRT) proteins.
abstract: ESCRT (Endosomal Sorting Complex Required for Transport) proteins are required for the sorting of biosynthetic and endocytic proteins at multivesicular bodies (MVBs). Here, I describe an assay to evaluate conservation of endosomal sorting functions of plant ESCRT proteins by trans-species complementation analysis. The assay is based on the imaging of a fluorescent biosynthetic MVB cargo, the carboxypeptidase S (CPS) fused to the green fluorescent protein GFP, in yeast ESCRT mutants expressing putative plant orthologues of the missing yeast ESCRT components.
authors.affiliation: Facultad Ciencias Biológicas, Centro de Biotecnología Vegetal, Universidad Andrés Bello, República 217, Santiago, Chile, francisca.reyes.marquez@gmail.com.
authors.forename: Francisca C
authors.initials: FC
authors.lastname: Reyes
mesh_terms: D000595:Amino Acid Sequence; D056827:Endosomal Sorting Complexes Required for Transport / Q000235:genetics / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D008967:Molecular Biology / Q000379:methods*; D021381:Protein Transport / Q000235:genetics; D033921:Vesicular Transport Proteins / Q000378:metabolism
chemical_list: D056827:Endosomal Sorting Complexes Required for Transport; D033921:Vesicular Transport Proteins; D049452:Green Fluorescent Proteins