title: Chiral speciation of selenoamino acids in biological samples.
abstract: In this paper, the "state of the art" of chiral speciation of selenoamino acids (SeAAs) in biological samples is critically reviewed. The significance and the features of such studies are highlighted. A special focus lies on chiral speciation of SeAAs by hyphenation techniques in which a chiral separation method (such as gas chromatography (GC), high performance liquid chromatography (HPLC) and capillary electrophoresis (CE)) is on-line coupled with an elemental specific detector, especially inductively coupled plasma mass spectrometry (ICP-MS). The advances in the development and application of hyphenation techniques in chiral speciation of SeAAs in biological samples are summarized and a perspective for future developments including sophisticated and innovative applications is discussed. Overall, HPLC-ICP-MS is more applicable than GC/CE-ICP-MS for chiral speciation of SeAAs. In the future, more novel chiral HPLC methods with high enantio-resolution, low cost and robustness, and their more applications in real biological samples analysis are expected.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Chen
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
authors.forename: Man
authors.initials: M
authors.lastname: He
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhong
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China. Electronic address: binhu@whu.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Hu
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods*; D012643:Selenium / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D012643:Selenium
keywords: Chiral speciation; Enantiomer; Hyphenation technique; Review; Selenoamino acid