title: Expression profile based gene clusters for ischemic stroke detection.
abstract: In microarray studies alterations in gene expression in circulating leukocytes have shown utility for ischemic stroke diagnosis. We studied forty candidate markers identified in three gene expression profiles to (1) quantitate individual transcript expression, (2) identify transcript clusters and (3) assess the clinical diagnostic utility of the clusters identified for ischemic stroke detection. Using high throughput next generation qPCR 16 of the 40 transcripts were significantly up-regulated in stroke patients relative to control subjects (p<0.05). Six clusters of between 5 and 7 transcripts were identified that discriminated between stroke and control (p values between 1.01e-9 and 0.03). A 7 transcript cluster containing PLBD1, PYGL, BST1, DUSP1, FOS, VCAN and FCGR1A showed high accuracy for stroke classification (AUC=0.854). These results validate and improve upon the diagnostic value of transcripts identified in microarray studies for ischemic stroke. The clusters identified show promise for acute ischemic stroke detection.
authors.affiliation: Department of Neurology, SUNY Downstate Medical Center, 450 Clarkson Ave., Brooklyn, NY 11203, USA; Department of Neurology, Jagiellonian University Medical College, ul. Botaniczna 3, Krakow 31-501, Poland.
authors.forename: Mateusz G
authors.initials: MG
authors.lastname: Adamski
authors.affiliation: Department of Neurology, SUNY Downstate Medical Center, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Neurology, SUNY Downstate Medical Center, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
authors.forename: Erin
authors.initials: E
authors.lastname: Wagner
authors.affiliation: Department of Neurology, SUNY Downstate Medical Center, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
authors.forename: Hua
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Neurology, SUNY Downstate Medical Center, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
authors.forename: Chloe
authors.initials: C
authors.lastname: Seales-Bailey
authors.affiliation: Department of Biomedical Engineering, University of North Carolina, 220 E Cameron Ave., Chapel Hill, NC 27514, USA; Department of Chemistry, University of North Carolina, 220 E Cameron Ave., Chapel Hill, NC27514, USA.
authors.forename: Steven A
authors.initials: SA
authors.lastname: Soper
authors.affiliation: Department of Mechanical Engineering, Louisiana State University, 3357 Highland Rd., Baton Rouge, LA 70802, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Murphy
authors.affiliation: Department of Neurology, SUNY Downstate Medical Center, 450 Clarkson Ave., Brooklyn, NY 11203, USA. Electronic address: alison.baird@downstate.edu.
authors.forename: Alison E
authors.initials: AE
authors.lastname: Baird
mesh_terms: D036541:ADP-ribosyl Cyclase / Q000235:genetics / Q000378:metabolism; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002545:Brain Ischemia / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D054638:Dual Specificity Phosphatase 1 / Q000235:genetics / Q000378:metabolism; D005260:Female; D058851:GPI-Linked Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D025001:Glycogen Phosphorylase, Liver Form / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D005810:Multigene Family; D010739:Phospholipase D / Q000235:genetics / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017452:Receptors, IgG / Q000235:genetics / Q000378:metabolism; D020521:Stroke / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome; D053675:Versicans / Q000235:genetics / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; C489017:FCGR1A protein, human; D058851:GPI-Linked Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D017452:Receptors, IgG; C508322:VCAN protein, human; D053675:Versicans; D025001:Glycogen Phosphorylase, Liver Form; C517019:DUSP1 protein, human; D054638:Dual Specificity Phosphatase 1; D010739:Phospholipase D; C106801:phospholipase D1; D036541:ADP-ribosyl Cyclase; C087521:ADP-ribosyl cyclase 2
keywords: Biomarker; Gene expression; Stroke; Transcriptome; qPCR