title: Ethanolic extract of Ferula gummosa is cytotoxic against cancer cells by inducing apoptosis and cell cycle arrest.
abstract: Ferula gummosa Boiss. has medicinal applications in treating a wide range of diseases including cancer. The objective of this study was to evaluate the antiproliferative activities of the seed and gum extracts of F. gummosa as well as to study the effect of the potent extract on the induction of apoptosis and cell cycle arrest. Our results demonstrated that the ethanolic extract had the lowest IC50 value at 72 h (0.001 ± 1.2 mg/mL) in BHY cells. Moreover, flowcytometry and annexin-V analysis revealed that the ethanolic extract induced apoptosis and cell-cycle arrest in BHY cells at G1/S phase. In addition, colorimetric methods exhibited the highest amount of total phenolics and flavonoids in the aqueous and gum extracts (0.12 ± 0.037, 0.01 ± 2.51 mg/g of dry powder). Generally, the results obtained indicate that F. gummosa ethanol extract may contain effective compounds which can be used as a chemotherapeutic agent.
authors.affiliation: a Department of Biology , Faculty of Science, Kharazmi University , Tehran , Iran.
authors.forename: Hoda
authors.initials: H
authors.lastname: Gudarzi
authors.forename: Mona
authors.initials: M
authors.lastname: Salimi
authors.forename: Saeed
authors.initials: S
authors.lastname: Irian
authors.forename: Amir
authors.initials: A
authors.lastname: Amanzadeh
authors.forename: Hirsa
authors.initials: H
authors.lastname: Mostafapour Kandelous
authors.forename: Keyhan
authors.initials: K
authors.lastname: Azadmanesh
authors.forename: Misha
authors.initials: M
authors.lastname: Salimi
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D045744:Cell Line, Tumor; D005309:Ferula / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry; D006801:Humans; D020128:Inhibitory Concentration 50; D010636:Phenols / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts
keywords: Ferula gummosa; MTT assay; apoptosis; cancer; flowcytometry