title: Screening of recombinant Escherichia coli using activation of green fluorescent protein as an indicator.
abstract: A novel cloning vector that can be used to identify recombinant Escherichia coli colonies by activation of the green fluorescent protein gene (GFP) was constructed. Screening using the vector does not require special reagents. The recombinant plasmid activates GFP, and the rate of false-positive results is low.
authors.affiliation: Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, Japan. Electronic address: tsuchida@hoku-iryo-u.ac.jp.
authors.forename: Shirou
authors.initials: S
authors.lastname: Tsuchida
authors.affiliation: Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
authors.forename: Mao
authors.initials: M
authors.lastname: Tamura
authors.affiliation: Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Hamaue
authors.affiliation: Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics*; D011995:Recombination, Genetic
chemical_list: D017931:DNA Primers; D049452:Green Fluorescent Proteins
keywords: Cloning vector; Escherichia coli; Green fluorescent protein