title: Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model.
abstract: OBJECTIVE
  To investigate whether granulocyte-colony stimulating factor (G-CSF) can decrease the extent of ovarian follicle loss caused by cisplatin treatment.
  
  
  METHODS
  Twenty-one adult female Sprague-Dawley rats were used. Fourteen rats were administered 2 mg/kg/day cisplatin by intraperitoneal injection twice per week for five weeks (total of 20 mg/kg). Half of the rats (n=7) were treated with 1 mL/kg/day physiological saline, and the other half (n=7) were treated with 100 μg/kg/day G-CSF. The remaining rats (n=7, control group) received no therapy. The animals were then euthanized, and both ovaries were obtained from all animals, fixed in 10% formalin, and stored at 4°C for paraffin sectioning. Blood samples were collected by cardiac puncture and stored at -30°C for hormone assays.
  
  
  RESULTS
  All follicle counts (primordial, primary, secondary, and tertiary) and serum anti-Müllerian hormone levels were significantly increased in the cisplatin+G-CSF group compared to the cisplatin+physiological saline group.
  
  
  CONCLUSIONS
  G-CSF was beneficial in decreasing the severity of follicle loss in an experimental rat model of cisplatin chemotherapy.
authors.affiliation: Department of Obstetrics and Gynecology, Ege University School of Medicine, Izmir, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Akdemir
authors.affiliation: Department of Obstetrics and Gynecology, Ege University School of Medicine, Izmir, Turkey. burak.zeybek@ege.edu.tr.
authors.forename: Burak
authors.initials: B
authors.lastname: Zeybek
authors.affiliation: Department of Obstetrics and Gynecology, Ege University School of Medicine, Izmir, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Akman
authors.affiliation: Department of Obstetrics and Gynecology, Ege University School of Medicine, Izmir, Turkey.
authors.forename: Ahment Mete
authors.initials: AM
authors.lastname: Ergenoglu
authors.affiliation: Department of Obstetrics and Gynecology, Ege University School of Medicine, Izmir, Turkey.
authors.forename: Ahmet Ozgur
authors.initials: AO
authors.lastname: Yeniel
authors.affiliation: Department of Physiology, Ege University School of Medicine, Izmir, Turkey.
authors.forename: Oytun
authors.initials: O
authors.lastname: Erbas
authors.affiliation: Department of Histology and Embryology, Ege University School of Medicine, Izmir, Turkey.
authors.forename: Altug
authors.initials: A
authors.lastname: Yavasoglu
authors.affiliation: Department of Obstetrics and Gynecology, Ege University School of Medicine, Izmir, Turkey.
authors.forename: Mustafa Cosan
authors.initials: MC
authors.lastname: Terek
authors.affiliation: Department of Physiology, Ege University School of Medicine, Izmir, Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Taskiran
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone / Q000097:blood; D000970:Antineoplastic Agents / Q000633:toxicity*; D015415:Biomarkers / Q000097:blood; D002945:Cisplatin / Q000633:toxicity*; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005260:Female; D059247:Fertility Preservation / Q000379:methods; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D006080:Ovarian Follicle / Q000187:drug effects / Q000473:pathology; D016649:Primary Ovarian Insufficiency / Q000097:blood / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D017207:Rats, Sprague-Dawley
chemical_list: D000970:Antineoplastic Agents; D015415:Biomarkers; D016179:Granulocyte Colony-Stimulating Factor; D054304:Anti-Mullerian Hormone; D002945:Cisplatin
keywords: Anti-Müllerian hormone; Cisplatin; Granulocyte-colony stimulating factor; Ovarian follicle; Ovary