title: Chemical composition, leishmanicidal and cytotoxic activities of the essential oils from Mangifera indica L. var. Rosa and Espada.
abstract: The essential oils from Mangifera indica var. Rosa and Espada latex were obtained by hydrodistillation and analyzed using GC-FID and GC-MS. Twenty-seven components were identified. The main compound in the essential oil from M. indica var. Espada (EOMiE) was terpinolene (73.6%). The essential oil of M. indica var. Rosa (EOMiR) was characterized by high amounts of β-pinene (40.7%) and terpinolene (28.3%). In the test for leishmanicidal activity against promastigotes forms of L. amazonensis, EOMiR and EOMiE showed IC50 (72 h) of 39.1 and 23.0 μg/mL, respectively. In macrophages, EOMiR and EOMiE showed CC50 of 142.84 and 158.65 μg/mL, respectively. However, both were more specific to the parasite than macrophages, with values of selectivity index of 6.91 for EOMiE and 3.66 for EOMiR. The essential oils were evaluated for their cytotoxicity against the human tumor cells HEp-2, HT-29, NCI-H292, and HL-60. The EOMiR and EOMiE were most effective against the HL-60, with IC50 values of 12.3 and 3.6 μg/mL, respectively. The results demonstrated that the essential oils of M. indica can destroy L. amazonensis and inhibit tumor cell growth. These findings contribute to the knowledge of the Brazilian biodiversity as a source of potential therapeutic agents.
authors.affiliation: Departamento de Antibióticos, Universidade Federal de Pernambuco, 50670-420 Recife, PE, Brazil.
authors.forename: Eduardo H S
authors.initials: EH
authors.lastname: Ramos
authors.affiliation: Departamento de Ciências Moleculares, Universidade Federal Rural de Pernambuco, 52171-900 Recife, PE, Brazil.
authors.forename: Marcílio M
authors.initials: MM
authors.lastname: Moraes
authors.affiliation: Departamento de Antibióticos, Universidade Federal de Pernambuco, 50670-420 Recife, PE, Brazil.
authors.forename: Laís L de A
authors.initials: LL
authors.lastname: Nerys
authors.affiliation: Departamento de Antibióticos, Universidade Federal de Pernambuco, 50670-420 Recife, PE, Brazil.
authors.forename: Silene C
authors.initials: SC
authors.lastname: Nascimento
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal de Pernambuco, 50670-420 Recife, PE, Brazil.
authors.forename: Gardênia C G
authors.initials: GC
authors.lastname: Militão
authors.affiliation: Departamento de Microbiologia, Centro de Pesquisa Aggeu Magalh, FIOCRUZ, 50670-420 Recife, PE, Brazil.
authors.forename: Regina C B Q
authors.initials: RC
authors.lastname: de Figueiredo
authors.affiliation: Departamento de Ciências Moleculares, Universidade Federal Rural de Pernambuco, 52171-900 Recife, PE, Brazil.
authors.forename: Cláudio A G
authors.initials: CA
authors.lastname: da Câmara
authors.affiliation: Departamento de Antibióticos, Universidade Federal de Pernambuco, 50670-420 Recife, PE, Brazil.
authors.forename: Teresinha Gonçalves
authors.initials: TG
authors.lastname: Silva
mesh_terms: D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds / Q000737:chemistry / Q000302:isolation & purification; D049109:Cell Proliferation / Q000187:drug effects; D000081005:Cyclohexane Monoterpenes; D019073:HT29 Cells; D006801:Humans; D007891:Leishmania / Q000187:drug effects*; D007896:Leishmaniasis / Q000188:drug therapy / Q000473:pathology; D031022:Mangifera / Q000737:chemistry*; D039821:Monoterpenes / Q000737:chemistry / Q000302:isolation & purification; D009369:Neoplasms / Q000188:drug therapy / Q000473:pathology; D009822:Oils, Volatile / Q000008:administration & dosage* / Q000737:chemistry*; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds; D000081005:Cyclohexane Monoterpenes; D039821:Monoterpenes; D009822:Oils, Volatile; D013729:Terpenes; C010789:beta-pinene; C027009:terpinolene