title: Multiplexed aberration measurement for deep tissue imaging in vivo.
abstract: We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine the sample-induced aberration. Applicable to fluorescent protein-labeled structures of arbitrary complexity, it allowed us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improved structural and functional imaging of fine neuronal processes over a large imaging volume.
authors.affiliation: 1] Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA. [2] State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai, China. [3].
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: 1] Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA. [2].
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: 1] Coleman Technologies, Inc., Newtown Square, Pennsylvania, USA. [2].
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Milkie
authors.affiliation: Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Sun
authors.affiliation: Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors.forename: Zhongchao
authors.initials: Z
authors.lastname: Tan
authors.affiliation: Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors.forename: Aaron
authors.initials: A
authors.lastname: Kerlin
authors.affiliation: Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors.forename: Tsai-Wen
authors.initials: TW
authors.lastname: Chen
authors.affiliation: Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors.forename: Douglas S
authors.initials: DS
authors.lastname: Kim
authors.affiliation: Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors.forename: Na
authors.initials: N
authors.lastname: Ji
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D017173:Caenorhabditis elegans; D005456:Fluorescent Dyes / Q000737:chemistry; D005583:Fourier Analysis; D006657:Histones / Q000737:chemistry; D008027:Light; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence / Q000379:methods; D059906:Neuroimaging / Q000379:methods*; D055095:Optics and Photonics; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry; D011680:Pupil / Q000502:physiology; D014793:Visual Cortex / Q000502:physiology; D015027:Zebrafish
chemical_list: D005456:Fluorescent Dyes; D006657:Histones; D011506:Proteins