title: Efficient quantification and characterization of bacterial outer membrane derived nano-particles with flow cytometric analysis.
abstract: There currently exists no efficient and easy method for size profiling and counting of membranous nano-scale particles, such as bacterial outer membrane vesicles (OMVs). We present here a cost-effective and fast method capable of profiling and counting small sample volumes of nano-scale membranous vesicles with standard laboratory equipment without the need for any washing steps. OMV populations of different bacterial species are compared and even subpopulations of OMVs can be identified after a simple labelling procedure. Counting is possible over three orders of magnitude without any changes to the protocol. Protein contaminations do not alter the described measurements.
authors.affiliation: Department of Bacteriology, Max von Pettenkofer Institute (LMU), Munich, Germany; Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany; German Center for Infection Reserach, Partner Site Munich, Munich, Germany; College of Public Health and Medical Science, Jimma University, Jimma, Ethiopia. Electronic address: wieser@mvp.uni-muenchen.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Wieser
authors.affiliation: Department of Bacteriology, Max von Pettenkofer Institute (LMU), Munich, Germany.
authors.forename: Enno
authors.initials: E
authors.lastname: Storz
authors.affiliation: Department of Bacteriology, Max von Pettenkofer Institute (LMU), Munich, Germany.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Liegl
authors.affiliation: Department of Bacteriology, Max von Pettenkofer Institute (LMU), Munich, Germany.
authors.forename: Annabell
authors.initials: A
authors.lastname: Peter
authors.affiliation: Department of Bacteriology, Max von Pettenkofer Institute (LMU), Munich, Germany; Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Munich, Germany; German Center for Infection Reserach, Partner Site Munich, Munich, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Pritsch
authors.affiliation: Laboratory for Quantum Gravity and Strings, Department of Mathematics and Applied Mathematics, University of Cape Town, Cape Town, South Africa.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Shock
authors.affiliation: Department of Molecular Biology and the Laboratory for Molecular Infection Medicine, Sweden (MIMS), Umeå University, Umeå SE-90187, Sweden.
authors.forename: Sun Nyunt
authors.initials: SN
authors.lastname: Wai
authors.affiliation: Department of Bacteriology, Max von Pettenkofer Institute (LMU), Munich, Germany.
authors.forename: Sören
authors.initials: S
authors.lastname: Schubert
mesh_terms: D001419:Bacteria / Q000737:chemistry*; D001431:Bacteriological Techniques / Q000379:methods; D055252:Cell-Derived Microparticles / Q000737:chemistry*; D005434:Flow Cytometry / Q000379:methods*; D053758:Nanoparticles / Q000032:analysis*
keywords: Escherichia coli; Flow cytometry; OMV; OMV enumeration; OMV quantification; Outer membrane vesicles