title: TBHP-induced oxidative stress alters microRNAs expression in mouse testis.
abstract: OBJECTIVE
  Reactive oxygen species (ROS) and oxidative stress is one of the main reasons of male infertility. MicroRNAs (miRNAs) regulate multiple intracellular processes. Alterations in miRNAs expression may occur in different conditions and diseases. In this study, the effect of oxidative stress induced by tertiary-butyl hydroperoxide (TBHP) on the expression of candidate miRNAs in mouse testis was investigated.
  
  
  METHODS
  After determining median lethal dose (LD50), TBHP was intraperitoneally (ip) injected at the dilution of 1:10 LD50 into the adult male mice for 2 weeks, and then testis tissues were removed in order to assay the ROS level. Total RNA was extracted and the expression of five miRNAs was quantified by reverse transcription-real time polymerase chain reaction (RT-qPCR).
  
  
  RESULTS
  The flow cytometry analysis showed a significant increase in ROS level in testis. The expression of three out of five selected miRNAs, including miR-34a, miR-181b and miR-122a, showed some degrees of changes following exposure to oxidative stress. These miRNAs are involved in antioxidant responses, inflammation pathway and spermatogenesis arrest.
  
  
  CONCLUSIONS
  In conclusion, TBHP alters the miRNA expression profile of testis which might play a potential role in oxidative and antioxidative responses and spermatogenesis.
authors.affiliation: Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, P.O. Box: 19395-4644, Tehran, Iran.
authors.forename: Nayeralsadat
authors.initials: N
authors.lastname: Fatemi
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Sanati
authors.forename: Mehdi
authors.initials: M
authors.lastname: Shamsara
authors.forename: Fariborz
authors.initials: F
authors.lastname: Moayer
authors.forename: Mansour Jamali
authors.initials: MJ
authors.lastname: Zavarehei
authors.forename: Alireza
authors.initials: A
authors.lastname: Pouya
authors.forename: ForoughAzam
authors.initials: F
authors.lastname: Sayyahpour
authors.forename: Hoda
authors.initials: H
authors.lastname: Ayat
authors.forename: Hamid
authors.initials: H
authors.lastname: Gourabi
mesh_terms: D000818:Animals; D007248:Infertility, Male / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics*; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D013091:Spermatogenesis / Q000187:drug effects / Q000235:genetics; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000494:pharmacology*
chemical_list: D035683:MicroRNAs; D017382:Reactive Oxygen Species; D020122:tert-Butylhydroperoxide