title: UCP1 is present in porcine adipose tissue and is responsive to postnatal leptin.
abstract: Intrauterine growth restriction (IUGR) may be accompanied by inadequate thermoregulation, especially in piglets that are not considered to possess any brown adipose tissue (BAT) and are thus entirely dependent on shivering thermogenesis in order to maintain body temperature after birth. Leptin can stimulate heat production by promoting non-shivering thermogenesis in BAT, but whether this response occurs in piglets is unknown. Newborn female piglets that were characterised as showing IUGR (mean birth weight of approximately 0.98 kg) were therefore administered injections of either saline or leptin once a day for the first 5 days of neonatal life. The dose of leptin was 0.5 mg/kg, which is sufficient to increase plasma leptin by approximately tenfold and on the day of birth induced a rapid increase in body temperature to values similar to those of normal-sized 'control' piglets (mean birth weight of ∼1.47 kg). Perirenal adipose tissue was then sampled from all offspring at 21 days of age and the presence of the BAT-specific uncoupling protein 1 (UCP1) was determined by immunohistochemistry and immunoblotting. UCP1 was clearly detectable in all samples analysed and its abundance was significantly reduced in the IUGR piglets that had received saline compared with controls, but was raised to the same amount as in controls in those IUGR females given leptin. There were no differences in gene expression between primary markers of brown and white adipose tissues between groups. In conclusion, piglets possess BAT that when stimulated exogenously by leptin can promote increased body temperature.
authors.affiliation: UP 2012.10.101 EGEALInstitut Polytechnique LaSalle, Beauvais, FranceSchool of Veterinary Medicine and ScienceUniversity of Nottingham, Sutton Bonington Campus, LE12 5RD Nottingham, UKINRA UMR 703Ecole Nationale Vétérinaire, Nantes, FranceINRAUMR1198 BDR Biologie du Développement et Reproduction, Jouy-en-Josas, FranceUnité de Recherche 04UR08/03Faculté de Médecine, Tunis, TunisiaThe Hebrew University of JerusalemPO Box 12, Rehovot 76100, IsraelUnité NOPAINRA, Centre de recherche Jouy en Josas, Jouy-en-Josas, FranceEarly Life Research UnitAcademic Child Health, School of Medicine, University Hospital, The University of Nottingham, Nottingham NG7 2UH, UK.
authors.forename: Alison
authors.initials: A
authors.lastname: Mostyn
authors.affiliation: UP 2012.10.101 EGEALInstitut Polytechnique LaSalle, Beauvais, FranceSchool of Veterinary Medicine and ScienceUniversity of Nottingham, Sutton Bonington Campus, LE12 5RD Nottingham, UKINRA UMR 703Ecole Nationale Vétérinaire, Nantes, FranceINRAUMR1198 BDR Biologie du Développement et Reproduction, Jouy-en-Josas, FranceUnité de Recherche 04UR08/03Faculté de Médecine, Tunis, TunisiaThe Hebrew University of JerusalemPO Box 12, Rehovot 76100, IsraelUnité NOPAINRA, Centre de recherche Jouy en Josas, Jouy-en-Josas, FranceEarly Life Research UnitAcademic Child Health, School of Medicine, University Hospital, The University of Nottingham, Nottingham NG7 2UH, UK.
authors.forename: Linda
authors.initials: L
authors.lastname: Attig
authors.affiliation: UP 2012.10.101 EGEALInstitut Polytechnique LaSalle, Beauvais, FranceSchool of Veterinary Medicine and ScienceUniversity of Nottingham, Sutton Bonington Campus, LE12 5RD Nottingham, UKINRA UMR 703Ecole Nationale Vétérinaire, Nantes, FranceINRAUMR1198 BDR Biologie du Développement et Reproduction, Jouy-en-Josas, FranceUnité de Recherche 04UR08/03Faculté de Médecine, Tunis, TunisiaThe Hebrew University of JerusalemPO Box 12, Rehovot 76100, IsraelUnité NOPAINRA, Centre de recherche Jouy en Josas, Jouy-en-Josas, FranceEarly Life Research UnitAcademic Child Health, School of Medicine, University Hospital, The University of Nottingham, Nottingham NG7 2UH, UK.
authors.forename: Thibaut
authors.initials: T
authors.lastname: Larcher
authors.affiliation: UP 2012.10.101 EGEALInstitut Polytechnique LaSalle, Beauvais, FranceSchool of Veterinary Medicine and ScienceUniversity of Nottingham, Sutton Bonington Campus, LE12 5RD Nottingham, UKINRA UMR 703Ecole Nationale Vétérinaire, Nantes, FranceINRAUMR1198 BDR Biologie du Développement et Reproduction, Jouy-en-Josas, FranceUnité de Recherche 04UR08/03Faculté de Médecine, Tunis, TunisiaThe Hebrew University of JerusalemPO Box 12, Rehovot 76100, IsraelUnité NOPAINRA, Centre de recherche Jouy en Josas, Jouy-en-Josas, FranceEarly Life Research UnitAcademic Child Health, School of Medicine, University Hospital, The University of Nottingham, Nottingham NG7 2UH, UK.
authors.forename: Samir
authors.initials: S
authors.lastname: Dou
authors.affiliation: UP 2012.10.101 EGEALInstitut Polytechnique LaSalle, Beauvais, FranceSchool of Veterinary Medicine and ScienceUniversity of Nottingham, Sutton Bonington Campus, LE12 5RD Nottingham, UKINRA UMR 703Ecole Nationale Vétérinaire, Nantes, FranceINRAUMR1198 BDR Biologie du Développement et Reproduction, Jouy-en-Josas, FranceUnité de Recherche 04UR08/03Faculté de Médecine, Tunis, TunisiaThe Hebrew University of JerusalemPO Box 12, Rehovot 76100, IsraelUnité NOPAINRA, Centre de recherche Jouy en Josas, Jouy-en-Josas, FranceEarly Life Research UnitAcademic Child Health, School of Medicine, University Hospital, The University of Nottingham, Nottingham NG7 2UH, UK.
authors.forename: Pascale
authors.initials: P
authors.lastname: Chavatte-Palmer
authors.affiliation: UP 2012.10.101 EGEALInstitut Polytechnique LaSalle, Beauvais, FranceSchool of Veterinary Medicine and ScienceUniversity of Nottingham, Sutton Bonington Campus, LE12 5RD Nottingham, UKINRA UMR 703Ecole Nationale Vétérinaire, Nantes, FranceINRAUMR1198 BDR Biologie du Développement et Reproduction, Jouy-en-Josas, FranceUnité de Recherche 04UR08/03Faculté de Médecine, Tunis, TunisiaThe Hebrew University of JerusalemPO Box 12, Rehovot 76100, IsraelUnité NOPAINRA, Centre de recherche Jouy en Josas, Jouy-en-Josas, FranceEarly Life Research UnitAcademic Child Health, School of Medicine, University Hospital, The University of Nottingham, Nottingham NG7 2UH, UK.
authors.forename: Monia
authors.initials: M
authors.lastname: Boukthir
authors.affiliation: UP 2012.10.101 EGEALInstitut Polytechnique LaSalle, Beauvais, FranceSchool of Veterinary Medicine and ScienceUniversity of Nottingham, Sutton Bonington Campus, LE12 5RD Nottingham, UKINRA UMR 703Ecole Nationale Vétérinaire, Nantes, FranceINRAUMR1198 BDR Biologie du Développement et Reproduction, Jouy-en-Josas, FranceUnité de Recherche 04UR08/03Faculté de Médecine, Tunis, TunisiaThe Hebrew University of JerusalemPO Box 12, Rehovot 76100, IsraelUnité NOPAINRA, Centre de recherche Jouy en Josas, Jouy-en-Josas, FranceEarly Life Research UnitAcademic Child Health, School of Medicine, University Hospital, The University of Nottingham, Nottingham NG7 2UH, UK.
authors.forename: Arieh
authors.initials: A
authors.lastname: Gertler
authors.affiliation: UP 2012.10.101 EGEALInstitut Polytechnique LaSalle, Beauvais, FranceSchool of Veterinary Medicine and ScienceUniversity of Nottingham, Sutton Bonington Campus, LE12 5RD Nottingham, UKINRA UMR 703Ecole Nationale Vétérinaire, Nantes, FranceINRAUMR1198 BDR Biologie du Développement et Reproduction, Jouy-en-Josas, FranceUnité de Recherche 04UR08/03Faculté de Médecine, Tunis, TunisiaThe Hebrew University of JerusalemPO Box 12, Rehovot 76100, IsraelUnité NOPAINRA, Centre de recherche Jouy en Josas, Jouy-en-Josas, FranceEarly Life Research UnitAcademic Child Health, School of Medicine, University Hospital, The University of Nottingham, Nottingham NG7 2UH, UK.
authors.forename: Jean
authors.initials: J
authors.lastname: Djiane
authors.affiliation: UP 2012.10.101 EGEALInstitut Polytechnique LaSalle, Beauvais, FranceSchool of Veterinary Medicine and ScienceUniversity of Nottingham, Sutton Bonington Campus, LE12 5RD Nottingham, UKINRA UMR 703Ecole Nationale Vétérinaire, Nantes, FranceINRAUMR1198 BDR Biologie du Développement et Reproduction, Jouy-en-Josas, FranceUnité de Recherche 04UR08/03Faculté de Médecine, Tunis, TunisiaThe Hebrew University of JerusalemPO Box 12, Rehovot 76100, IsraelUnité NOPAINRA, Centre de recherche Jouy en Josas, Jouy-en-Josas, FranceEarly Life Research UnitAcademic Child Health, School of Medicine, University Hospital, The University of Nottingham, Nottingham NG7 2UH, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: E Symonds
authors.affiliation: UP 2012.10.101 EGEALInstitut Polytechnique LaSalle, Beauvais, FranceSchool of Veterinary Medicine and ScienceUniversity of Nottingham, Sutton Bonington Campus, LE12 5RD Nottingham, UKINRA UMR 703Ecole Nationale Vétérinaire, Nantes, FranceINRAUMR1198 BDR Biologie du Développement et Reproduction, Jouy-en-Josas, FranceUnité de Recherche 04UR08/03Faculté de Médecine, Tunis, TunisiaThe Hebrew University of JerusalemPO Box 12, Rehovot 76100, IsraelUnité NOPAINRA, Centre de recherche Jouy en Josas, Jouy-en-Josas, FranceEarly Life Research UnitAcademic Child Health, School of Medicine, University Hospital, The University of Nottingham, Nottingham NG7 2UH, UK latifa.najar@lasalle-beauvais.fr.
authors.forename: Latifa
authors.initials: L
authors.lastname: Abdennebi-Najar
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism*; D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000378:metabolism; D052436:Adipose Tissue, White / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001831:Body Temperature / Q000187:drug effects; D004334:Drug Administration Schedule; D005260:Female; D005317:Fetal Growth Retardation / Q000378:metabolism; D015151:Immunoblotting; D007150:Immunohistochemistry; D007273:Injections, Intramuscular; D007473:Ion Channels / Q000378:metabolism*; D020738:Leptin / Q000008:administration & dosage / Q000494:pharmacology*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D013552:Swine; D022722:Thermogenesis / Q000187:drug effects; D000071256:Uncoupling Protein 1
chemical_list: D007473:Ion Channels; D020738:Leptin; D024101:Mitochondrial Proteins; D000071256:Uncoupling Protein 1
keywords: IUGR; UCP1; adipose tissue; leptin; porcine; programming