title: Proteomics of endosomal compartments from plants case study: isolation of trans-Golgi network vesicles.
abstract: A detailed understanding of endomembrane processes and their biological roles is vital for a complete picture of plant growth and development; however their highly dynamic nature has complicated comprehensive and rigorous studies so far. Recent pioneering efforts have demonstrated that isolation of vesicles in their native state, paired with a quantitative identification of their cargo, offers a viable and practicable approach for the dissection of endomembrane trafficking pathways. The protocol presented in this chapter describes in detail the isolation of the SYP61 trans-Golgi network vesicles from Arabidopsis. With minor alterations, in a few key parameters, it can be adopted to yield a universal procedure for the broad spectrum of plant vesicles.
authors.affiliation: Department of Plant sciences, University of California Davis, Asmundson Hall, One Shields Avenue, Davis, CA, 95616, USA.
authors.forename: Eunsook
authors.initials: E
authors.lastname: Park
authors.forename: Georgia
authors.initials: G
authors.lastname: Drakakaki
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000096:biosynthesis; D001692:Biological Transport; D011992:Endosomes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D008967:Molecular Biology / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D021601:trans-Golgi Network / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins