title: Histochemical staining using lectin probes.
abstract: In histochemistry and cytochemistry, lectins are often used as probes for the localization of carbohydrates in cells and tissues. With lectins, cells and tissues can be identified as a particular type or a group in situ. Various lectins have been used for mapping of normal cells and tissues, pathological diagnosis such as malignant transformation, and identification of cell lineages during development. This chapter describes light and electron microscopic methods using lectin probes for determining carbohydrate localization in cells and tissues.
authors.affiliation: Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo, 181-8611, Japan, yakimoto@ks.kyorin-u.ac.jp.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Akimoto
authors.forename: Hayato
authors.initials: H
authors.lastname: Kawakami
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D006651:Histocytochemistry / Q000379:methods*; D006735:Horseradish Peroxidase / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D008854:Microscopy, Electron; D015335:Molecular Probes / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*; D016610:Tissue Embedding; D016707:Tissue Fixation
chemical_list: D005456:Fluorescent Dyes; D037102:Lectins; D015335:Molecular Probes; D011134:Polysaccharides; D006735:Horseradish Peroxidase; D006026:Glycoside Hydrolases