title: Design, synthesis, and in vitro evaluation of an activity-based protein profiling (ABPP) probe targeting agmatine deiminases.
abstract: Agmatine deiminases (AgDs) belong to a family of enzymes known as guanidinium group modifying enzymes (GMEs). Many pathogenic bacteria encode an AgD that participates in the catabolism of agmatine (decarboxylated arginine). This catabolism may confer a competitive survival advantage, by virtue of energy production and increased acid tolerance, making this sub-family of enzymes a potential therapeutic target that warrants further study. Herein we report the development of an activity-based protein profiling (ABPP) probe that selectively targets the AgD from Streptococcus mutans. Due to the selectivity and covalent nature of the modification, this probe could prove to be a valuable tool for the study of other AgD family members.
authors.affiliation: Department of Chemistry, University of North Florida, 1 UNF Dr., Jacksonville, FL 32224, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Thomson
authors.affiliation: Department of Chemistry, University of North Florida, 1 UNF Dr., Jacksonville, FL 32224, USA.
authors.forename: Sean
authors.initials: S
authors.lastname: O'Connor
authors.affiliation: Department of Chemistry, University of North Florida, 1 UNF Dr., Jacksonville, FL 32224, USA.
authors.forename: Bryan
authors.initials: B
authors.lastname: Knuckley
authors.affiliation: Department of Chemistry, University of North Florida, 1 UNF Dr., Jacksonville, FL 32224, USA. Electronic address: corey.causey@unf.edu.
authors.forename: Corey P
authors.initials: CP
authors.lastname: Causey
mesh_terms: D000345:Affinity Labels / Q000138:chemical synthesis / Q000737:chemistry*; D015195:Drug Design; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry; D006867:Hydrolases / Q000737:chemistry* / Q000378:metabolism*; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D013237:Stereoisomerism; D013295:Streptococcus mutans / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D000345:Affinity Labels; D005456:Fluorescent Dyes; D015335:Molecular Probes; D006867:Hydrolases; C019626:agmatine deiminase
keywords: ABPP probe; Activity-based protein profiling; Agmatine catabolism; Agmatine deiminase