title: Nucleotidyl transferase TUT1 inhibits lipogenesis in osteosarcoma cells through regulation of microRNA-24 and microRNA-29a.
abstract: Osteosarcoma is the most common type of bone cancer. In the present study, by way of PCR-based microarrays, we found that TUT1, a nucleotidyl transferase, was significantly downregulated in osteosarcoma, compared with adjacent normal tissues. In the current study, we performed PCR-based microarrays using the cDNA prepared from osteosarcoma and adjacent normal tissues. The enforced expression of TUT1 was able to inhibit cell proliferation in U2OS and MG63 cells, while its knockdown using small interfering RNA (siRNA) oligos promoted cell proliferation. At the molecular level, we found that TUT1 could inhibit the expression levels of PPARgamma and SREBP-1c, two key regulators in lipogenesis, through upregulation of microRNA-24 and microRNA-29a. Therefore, our results suggest that TUT1 may act as a tumor suppressor for osteosarcoma, which might provide a novel mechanism for the tumor development.
authors.affiliation: Department of Pharmacy, Shanghai Tongji Hospital, 200065, Shanghai, China.
authors.forename: De-Qiu
authors.initials: DQ
authors.lastname: Zhu
authors.forename: Yue-Fen
authors.initials: YF
authors.lastname: Lou
authors.forename: Zhi-Gao
authors.initials: ZG
authors.lastname: He
authors.forename: Min
authors.initials: M
authors.lastname: Ji
mesh_terms: D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D050155:Lipogenesis / Q000235:genetics*; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D009713:Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D034622:RNA Interference; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics
chemical_list: C543845:MIRN24 microRNA, human; C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D009713:Nucleotidyltransferases; C586325:TUT1 protein, human