title: Identification of Angiostrongylus cantonensis and other nematodes using the SSU rDNA in Achatina fulica populations of Metro Manila.
abstract: Angiostrongylus cantonensis is a parasitic nematode that causes eosinophilic meningitis in humans. Accidental infection occurs by consumption of contaminated intermediates, such as the giant African land snail, Achatina fulica. This study surveyed the presence of A. cantonensis juveniles in A. fulica populations from 12 sites in Metropolitan Manila, Philippines using the SSU rDNA. Fourteen distinct sequences from 226 nematodes were obtained; of these, two matched A. cantonensis and Ancylostoma caninum, respectively, with 100% identity. Exact identities of the remaining twelve sequences could not be determined due to low percent similarities. Of the sequenced nematodes, A. cantonensis occurred with the highest frequency (139 out of 226). Most of these (131 out of 139) were collected in just one area in Quezon City. Nematode infection of A. fulica in this area and two others from Makati and another area in Quezon City, respectively, were highest, combining for 95% of the total infection. Ancylostoma caninum, on the other hand, was detected in four different sites. A. caninum is a canine parasite, and this is the first report of the nematode in A. fulica. These results cause public health concerns as both A. cantonensis and A. caninum are zoonotic to humans.
authors.affiliation: Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City, 1101, Philippines.
authors.forename: M A
authors.initials: MA
authors.lastname: Constantino-Santos
authors.affiliation: Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City, 1101, Philippines.
authors.forename: Z U
authors.initials: ZU
authors.lastname: Basiao
authors.affiliation: School of Biology, University Park, University of Nottingham, NG7 2RD, United Kingdom.
authors.forename: C M
authors.initials: CM
authors.lastname: Wade
authors.affiliation: Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City, 1101, Philippines.
authors.forename: B S
authors.initials: BS
authors.lastname: Santos
authors.affiliation: Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City, 1101, Philippines.
authors.forename: K C
authors.initials: KC
authors.lastname: Fontanilla I
mesh_terms: D000722:Ancylostoma / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D017185:Angiostrongylus cantonensis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D000818:Animals; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D049851:Gastropoda / Q000469:parasitology*; D008969:Molecular Sequence Data; D010679:Philippines; D058974:Phylogeography; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S