title: Genome-wide prediction of the polymorphic Ser gene family in Tetrahymena thermophila based on motif analysis.
abstract: Even though antigenic variation is employed among parasitic protozoa for host immune evasion, Tetrahymena thermophila, a free-living ciliate, can also change its surface protein antigens. These cysteine-rich glycosylphosphatidylinositol (GPI)-linked surface proteins are encoded by a family of polymorphic Ser genes. Despite the availability of T. thermophila genome, a comprehensive analysis of the Ser family is limited by its high degree of polymorphism. In order to overcome this problem, a new approach was adopted by searching for Ser candidates with common motif sequences, namely length-specific repetitive cysteine pattern and GPI anchor site. The candidate genes were phylogenetically compared with the previously identified Ser genes and classified into subtypes. Ser candidates were often found to be located as tandem arrays of the same subtypes on several chromosomal scaffolds. Certain Ser candidates located in the same chromosomal arrays were transcriptionally expressed at specific T. thermophila developmental stages. These Ser candidates selected by the motif analysis approach can form the foundation for a systematic identification of the entire Ser gene family, which will contribute to the understanding of their function and the basis of T. thermophila antigenic variation.
authors.affiliation: Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors.forename: Patrath
authors.initials: P
authors.lastname: Ponsuwanna
authors.affiliation: Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors.forename: Krittikorn
authors.initials: K
authors.lastname: Kümpornsin
authors.affiliation: Center of Excellence in Malaria, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Thanat
authors.initials: T
authors.lastname: Chookajorn
mesh_terms: D018503:Genome, Protozoan / Q000235:genetics*; D015800:Protozoan Proteins / Q000378:metabolism*; D016808:Tetrahymena thermophila / Q000235:genetics / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins