title: Simple and validated quantitative ¹H NMR method for the determination of methylation, acetylation, and feruloylation degree of pectin.
abstract: The knowledge of pectin esterification degree is of primary importance to predict gelling and other properties of pectin from different sources. This paper reports the development of a simple and rapid (1)H NMR-based method for the simultaneous quantitative determination of methylation, acetylation, and feruloylation degree of pectin isolated from various food sources. Pectin esters are hydrolyzed in NaOH/D2O, and the obtained methanol, acetic acid, and ferulic acid are directly measured by (1)H NMR. High accuracy, repeatability, and reproducibility of the method were obtained, and the analysis time is reduced as compared to conventional chromatography- or titration-based methods.
authors.affiliation: Department of Food Science, University of Parma , Parma, Italy.
authors.forename: Judith
authors.initials: J
authors.lastname: Müller-Maatsch
authors.forename: Augusta
authors.initials: A
authors.lastname: Caligiani
authors.forename: Tullia
authors.initials: T
authors.lastname: Tedeschi
authors.forename: Kathy
authors.initials: K
authors.lastname: Elst
authors.forename: Stefano
authors.initials: S
authors.lastname: Sforza
mesh_terms: D019342:Acetic Acid / Q000032:analysis; D000107:Acetylation; D003373:Coumaric Acids / Q000032:analysis; D004298:Dopamine / Q000031:analogs & derivatives; D004951:Esterification; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D000432:Methanol / Q000032:analysis; D008745:Methylation; D015394:Molecular Structure; D010368:Pectins / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D003373:Coumaric Acids; C525182:feruloyldopamine; D010368:Pectins; C004999:ferulic acid; D019342:Acetic Acid; D004298:Dopamine; D000432:Methanol
keywords: acetylation degree; feruloylation degree; methylation degree; pectin; quantitative 1H NMR