title: Circulating miR-192 in liver fluke-associated cholangiocarcinoma patients: a prospective prognostic indicator.
abstract: BACKGROUND
  This study aimed to investigate the miR-192 levels in patients' sera of liver fluke-associated cholangiocarcinoma (CCA) for a prospective prognostic indicator.
  
  
  METHODS
  MicroRNA polymerase chain reaction (PCR) array was performed using pooled serum samples from 11 CCA patients and nine healthy subjects. Selected miRNAs were verified for the differential levels in both sera and tumor tissues (of patients and Opisthorchis viverrini (Ov)-induced CCA model) using TaqMan miRNA expression assay.
  
  
  RESULTS
  Our results demonstrated that miR-192 was significantly higher in the serum of CCA patients than that in healthy subjects giving a sensitivity of 74% and specificity of 72% (area under the curve [AUC] = 0.803; 95% confidence interval [CI], 0.708-0.897, P < 0.0001). Serum miR-192 examined in Ov infected subjects and subjects with periductal fibrosis were increased but not statistically significantly when compared with healthy subjects. High levels of serum miR-192 were significantly correlated with lymph node metastasis (P = 0.047) and shorter survival compared with individuals with low levels of serum miR-192 (hazard ratio [HR] 2.076, 95% CI 1.004-4.291, P = 0.049). We also found that the expression levels of miR-192 appeared to be elevated in both CCA tissues of patients and in Ov-induced CCA tissues of a hamster model.
  
  
  CONCLUSIONS
  This finding indicates that elevated levels of miR-192 may be involved in CCA genesis and have a potential utility as a noninvasive prognostic indicator for CCA patients.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Khon Kaen University, 123 Mitraparb Road, Khon Kaen, 40002, Thailand; Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, Thailand. nisana@kku.ac.th.
authors.forename: Runglawan
authors.initials: R
authors.lastname: Silakit
authors.forename: Watcharin
authors.initials: W
authors.lastname: Loilome
authors.forename: Puangrat
authors.initials: P
authors.lastname: Yongvanit
authors.forename: Porncheera
authors.initials: P
authors.lastname: Chusorn
authors.forename: Anchalee
authors.initials: A
authors.lastname: Techasen
authors.forename: Thidarut
authors.initials: T
authors.lastname: Boonmars
authors.forename: Narong
authors.initials: N
authors.lastname: Khuntikeo
authors.forename: Nittaya
authors.initials: N
authors.lastname: Chamadol
authors.forename: Chawalit
authors.initials: C
authors.lastname: Pairojkul
authors.forename: Nisana
authors.initials: N
authors.lastname: Namwat
mesh_terms: D000818:Animals; D001650:Bile Duct Neoplasms / Q000097:blood / Q000235:genetics* / Q000469:parasitology*; D001653:Bile Ducts, Intrahepatic / Q000469:parasitology; D014408:Biomarkers, Tumor / Q000097:blood; D018281:Cholangiocarcinoma / Q000097:blood / Q000235:genetics* / Q000469:parasitology*; D005260:Female; D006801:Humans; D008297:Male; D008647:Mesocricetus; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D009889:Opisthorchiasis / Q000097:blood / Q000235:genetics* / Q000469:parasitology*; D009891:Opisthorchis; D011379:Prognosis; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction; D013785:Thailand
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: Cholangiocarcinoma; Opisthorchis viverrini; Prognostic indicator; Serum miR-192