title: LDAI-based chemical labeling of intact membrane proteins and its pulse-chase analysis under live cell conditions.
abstract: The functions of membrane proteins are tightly controlled by the dynamics such as protein trafficking and degradation. We demonstrated that ligand-directed acyl imidazole (LDAI) chemistry is broadly applicable to selective chemical labeling of various types of membrane-bound proteins under live cell conditions without a need for any tag fragments. The LDAI chemistry enabled pulse-chase analysis of these proteins to determine the half-life, as well as their degradation pathways by the imaging, under almost natural cellular conditions.
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Miki
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Sho-hei
authors.initials: SH
authors.lastname: Fujishima
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Komatsu
authors.affiliation: Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Chikusa, Nagoya 464-8602, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Kuwata
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Kiyonaka
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, 5 Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan. Electronic address: ihamachi@sbchem.kyoto-u.ac.jp.
authors.forename: Itaru
authors.initials: I
authors.lastname: Hamachi
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D006801:Humans; D007093:Imidazoles / Q000737:chemistry*; D008024:Ligands; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: D007093:Imidazoles; D008024:Ligands; D008565:Membrane Proteins