title: Experimental study of the impact of antimicrobial treatments on Campylobacter, Enterococcus and PCR-capillary electrophoresis single-strand conformation polymorphism profiles of the gut microbiota of chickens.
abstract: An experiment was conducted to compare the impact of antimicrobial treatments on the susceptibility of Campylobacter, Enterococcus faecium and Enterococcus faecalis, and on the diversity of broiler microbiota. Specific-pathogen-free chickens were first orally inoculated with strains of Campylobacter and Enterococcus faecium. Birds were then orally treated with recommended doses of oxytetracycline, sulfadimethoxine/trimethoprim, amoxicillin or enrofloxacin. Faecal samples were collected before, during and after antimicrobial treatment. The susceptibility of Campylobacter, Enterococcus faecium and Enterococcus faecalis strains isolated on supplemented or non-supplemented media was studied and PCR-capillary electrophoresis single-strand conformation polymorphism (CE-SSCP) profiles of the gut microbiota were analysed. Enrofloxacin-resistant Campylobacter were selected in the enrofloxacin-treated group and showed the Thr86Ile mutation in the gyrA gene. Acquisition of the tetO gene in Campylobacter coli isolates was significantly more frequent in birds given oxytetracycline. No impact of amoxicillin treatment on the susceptibility of Campylobacter could be detected. Ampicillin- and sulfadimethoxine/trimethoprim-resistant Enterococcus faecium were selected in amoxicillin-treated broilers, but no selection of the inoculated vancomycin-resistant Enterococcus faecium could be detected, although it was also resistant to tetracycline and sulfadimethoxine/trimethoprim. PCR-CE-SSCP revealed significant variations in a few peaks in treated birds as compared with non-treated chickens. In conclusion, antimicrobial treatments perturbed chicken gut microbiota, and certain antimicrobial treatments selected or co-selected resistant strains of Campylobacter and Enterococcus.
authors.affiliation: Université Européenne de Bretagne, Rennes, France.|ANSES, Ploufragan Laboratory, 22440 Ploufragan, France.
authors.forename: Gwenaëlle
authors.initials: G
authors.lastname: Mourand
authors.affiliation: Université Européenne de Bretagne, Rennes, France.|ANSES, Ploufragan Laboratory, 22440 Ploufragan, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Jouy
authors.affiliation: Université Européenne de Bretagne, Rennes, France.|ANSES, Ploufragan Laboratory, 22440 Ploufragan, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Bougeard
authors.affiliation: Université Européenne de Bretagne, Rennes, France.|ANSES, Ploufragan Laboratory, 22440 Ploufragan, France.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Dheilly
authors.affiliation: Université Européenne de Bretagne, Rennes, France.|ANSES, Ploufragan Laboratory, 22440 Ploufragan, France.
authors.forename: Annaëlle
authors.initials: A
authors.lastname: Kérouanton
authors.affiliation: Université Européenne de Bretagne, Rennes, France.|ANSES, Ploufragan Laboratory, 22440 Ploufragan, France.
authors.forename: Salman
authors.initials: S
authors.lastname: Zeitouni
authors.affiliation: Université Européenne de Bretagne, Rennes, France.|ANSES, Ploufragan Laboratory, 22440 Ploufragan, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Kempf
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002167:Campylobacter / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D002645:Chickens; D024881:Drug Resistance, Bacterial; D019075:Electrophoresis, Capillary / Q000379:methods / Q000662:veterinary; D016983:Enterococcus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D041981:Gastrointestinal Tract / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011110:Polymorphism, Genetic; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D059467:Transcriptome
chemical_list: D000900:Anti-Bacterial Agents; D012336:RNA, Ribosomal, 16S