title: Synthesis of composites SBA-15 mesoporous particles carrying oxytocin and evaluation of their properties, functions, and in vitro biological activities.
abstract: Using the organic template method, we have synthesized mesoporous SBA-15 particles and characterized them by scanning electron microscopy and transmission electron microscopy. The bone metabolism regulating hormone oxytocin (OT) was selected as a model for preparation of drug/SBA-15 complexes. The process of drug loading was studied using X-ray diffraction and nitrogen absorption methods. Optimal drug loading parameters were experimentally investigated. The kinetics of drug release from the carrier was evaluated. Finally, the extractions of SBA-15 particles were tested for cytotoxicity, in vitro hemolysis, and the direct attachment toxicity. Our findings suggest that SBA-15 materials have good biocompatibility. Moreover, we demonstrated that OT/SBA-15 complex can stimulate alkaline phosphatase activity in osteoblast cells. The study provides fundamental information for further in vivo drug-carrier testing.
authors.affiliation: Department of Prosthodontics, School of Stomatology, Jilin University, Changchun, 130021, Jilin, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Qian
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Mengna
authors.initials: M
authors.lastname: Duan
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wu
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000327:Adsorption; D000469:Alkaline Phosphatase / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D060326:Chemistry Techniques, Synthetic; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D065546:Drug Liberation; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D009584:Nitrogen / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D010121:Oxytocin / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D004337:Drug Carriers; C509969:SBA-15; D010121:Oxytocin; D012822:Silicon Dioxide; D000469:Alkaline Phosphatase; D009584:Nitrogen