title: Recipients with in utero induction of tolerance upregulated MHC class I in the engrafted donor skin.
abstract: The alterations in MHC class I expression play a crucial step in immune evasion of cancer or virus-infected cells. This study aimed to examine whether tolerized grafts modified MHC class I expression. FVB/N mice were rendered tolerant of C57BL/6 alloantigens by in utero transplantation of C57BL/6 marrows. Postnatally, engrafted donor skins and leukocytes were examined for their MHC expression by quantitative real-time PCR and flow cytometry. Engrafted donor skins upregulated their MHC class I related gene transcripts after short-term (1~2 weeks) or long-term (>1 month) engraftment. This biological phenomenon was simultaneously associated with upregulation of TAP1 gene transcripts, suggesting an important role of TAP1 in the regulation of MHC class I pathway. The surface MHC class I molecules of H-2K(b) in engrafted donor leukocytes consistently showed overexpression. Conclusively, the induction of allograft tolerance involved biological modifications of donor transplants. The overexpression of MHC class I within engrafted transplants of tolerant mice might be used as the tolerance biomarkers for identifying a state of graft tolerance.
authors.affiliation: Department of Surgery, Chang Gung Children's Hospital, College of Medicine, Chang Gung University, 5 Fu-Shin Street, Kweishan, Taoyuan 333, Taiwan.
authors.forename: Jeng-Chang
authors.identifier: 0000-0002-7776-4338
authors.initials: JC
authors.lastname: Chen
authors.affiliation: Department of Allergy, Asthma and Rheumatology, Chang Gung Children's Hospital, College of Medicine, Chang Gung University, 5 Fu-Shin Street, Kweishan, Taoyuan 333, Taiwan.
authors.forename: Liang-Shiou
authors.initials: LS
authors.lastname: Ou
authors.affiliation: Pediatric Research Center, Chang Gung Children's Hospital, 5 Fu-Shin Street, Kweishan, Taoyuan 333, Taiwan.
authors.forename: Hsiu-Yueh
authors.initials: HY
authors.lastname: Yu
authors.affiliation: Department of Microbiology and Immunology, Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 333, Taiwan.
authors.forename: Ming-Ling
authors.initials: ML
authors.lastname: Kuo
authors.affiliation: Department of Surgery, Chang Gung Children's Hospital, College of Medicine, Chang Gung University, 5 Fu-Shin Street, Kweishan, Taoyuan 333, Taiwan.
authors.forename: Pei-Yeh
authors.initials: PY
authors.lastname: Chang
authors.affiliation: Pediatric Research Center, Chang Gung Children's Hospital, 5 Fu-Shin Street, Kweishan, Taoyuan 333, Taiwan.
authors.forename: Hsueh-Ling
authors.initials: HL
authors.lastname: Chang
mesh_terms: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D000818:Animals; D016026:Bone Marrow Transplantation; D004622:Embryo, Mammalian / Q000276:immunology*; D005260:Female; D006085:Graft Survival; D015234:HLA-A Antigens / Q000235:genetics / Q000378:metabolism*; D007108:Immune Tolerance; D007962:Leukocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000276:immunology / Q000378:metabolism*; D016038:Skin Transplantation; D015854:Up-Regulation
chemical_list: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters; D015234:HLA-A Antigens; D012333:RNA, Messenger; C486171:Tap1 protein, mouse