title: Abarema cochliacarpos extract decreases the inflammatory process and skeletal muscle injury induced by Bothrops leucurus venom.
abstract: Snakebites are a public health problem, especially in tropical countries. However, treatment with antivenom has limited effectiveness against venoms' local effects. Here, we investigated the ability of Abarema cochliacarpos hydroethanolic extract (EAc) to protect mice against injection of Bothrops leucurus venom. Swiss mice received perimuscular venom injection and were subsequently treated orally with EAc in different doses. Treatment with EAc 100, 200, and 400 mg/kg reduced the edema induced by B. leucurus in 1%, 13%, and 39%, respectively. Although lower doses showed no antihypernociceptive effect in the Von Frey test, the higher dose significantly reduced hyperalgesia induced by the venom. Antimyotoxic activity of EAc was also observed by microscopy assessment, with treated muscles presenting preserved structures, decreased edema, and inflammatory infiltrate as compared to untreated ones. Finally, on the rotarod test, the treated mice showed better motor function, once muscle fibers were preserved and there were less edema and pain. Treated mice could stand four times more time on the rotating rod than untreated ones. Our results have shown that EAc presented relevant activities against injection of B. leucurus venom in mice, suggesting that it can be considered as an adjuvant in the treatment of envenomation.
authors.affiliation: Departamento de Fisiologia, Laboratorio de Farmacologia Pré-Clinica, Universidade Federal de Sergipe, SE, Brazil ; Departamento de Morfologia, Laboratório de Biologia Celular e Estrutura, Universidade Federal de Sergipe, SE, Brazil ; Programa de Pós-Graduação em Biotecnologia (RENORBIO), Universidade Federal de Sergipe, SE, Brazil.
authors.forename: Jeison
authors.initials: J
authors.lastname: Saturnino-Oliveira
authors.affiliation: Departamento de Fisiologia, Laboratorio de Farmacologia Pré-Clinica, Universidade Federal de Sergipe, SE, Brazil.
authors.forename: Daiana Do Carmo
authors.initials: Ddo C
authors.lastname: Santos
authors.affiliation: Departamento de Fisiologia, Laboratorio de Farmacologia Pré-Clinica, Universidade Federal de Sergipe, SE, Brazil.
authors.forename: Adriana Gibara
authors.initials: AG
authors.lastname: Guimarães
authors.affiliation: Departamento de Fisiologia, Laboratório de Bioquímica e Química de Produtos Naturais, SE, Brazil.
authors.forename: Antônio
authors.initials: A
authors.lastname: Santos Dias
authors.affiliation: Laboratório de Farmacologia das Toxinas, ICB, UFRJ, Universidade Federal do Rio de Janeiro, RJ, Brazil.
authors.forename: Marcelo Amorim
authors.initials: MA
authors.lastname: Tomaz
authors.affiliation: Laboratório de Farmacologia das Toxinas, ICB, UFRJ, Universidade Federal do Rio de Janeiro, RJ, Brazil.
authors.forename: Marcos
authors.initials: M
authors.lastname: Monteiro-Machado
authors.affiliation: Departamento de Fisiologia, Laboratório de Bioquímica e Química de Produtos Naturais, SE, Brazil.
authors.forename: Charles Santos
authors.initials: CS
authors.lastname: Estevam
authors.affiliation: Departamento de Morfologia, Laboratório de Biologia Celular e Estrutura, Universidade Federal de Sergipe, SE, Brazil.
authors.forename: Waldecy
authors.initials: W
authors.lastname: De Lucca Júnior
authors.affiliation: Instituto Butantan, Laboratório de Ciências Fisiológicas e Química, São Paulo, SP, Brazil.
authors.forename: Durvanei Augusto
authors.initials: DA
authors.lastname: Maria
authors.affiliation: Laboratório de Farmacologia das Toxinas, ICB, UFRJ, Universidade Federal do Rio de Janeiro, RJ, Brazil.
authors.forename: Paulo A
authors.initials: PA
authors.lastname: Melo
authors.affiliation: Departamento de Farmácia, Laboratório de Ensaios e de Toxicidade Farmacêutica, Universidade Federal de Sergipe, SE, Brazil.
authors.forename: Adriano Antunes de Souza
authors.initials: AA
authors.lastname: Araújo
authors.affiliation: Departamento de Fisiologia, Laboratorio de Farmacologia Pré-Clinica, Universidade Federal de Sergipe, SE, Brazil.
authors.forename: Márcio Roberto Viana
authors.initials: MR
authors.lastname: Santos
authors.affiliation: Colegiado de Ciências Farmacêuticas, Universidade Federal do Vale do São Francisco, PE, Brazil.
authors.forename: Jackson Roberto Guedes da Silva
authors.initials: JR
authors.lastname: Almeida
authors.affiliation: Departamento de Biofísica e Fisiologia, Universidade Federal do Piaui, Teresina, PI, Brazil.
authors.forename: Rita de Cássia Meneses
authors.initials: Rde C
authors.lastname: Oliveira
authors.affiliation: Departamento de Biofísica e Fisiologia, Universidade Federal do Piaui, Teresina, PI, Brazil.
authors.forename: Aldeidia
authors.initials: A
authors.lastname: Pereira de Oliveira
authors.affiliation: Departamento de Fisiologia, Laboratorio de Farmacologia Pré-Clinica, Universidade Federal de Sergipe, SE, Brazil ; Programa de Pós-Graduação em Biotecnologia (RENORBIO), Universidade Federal de Sergipe, SE, Brazil ; Laboratory Preclinical Pharmacology of Natural Products, Department of Physiology, Federal University of Sergipe, S/N Marechal Rondon Avenue, 49.100-000 São Cristovão, SE, Brazil.
authors.forename: Lucindo José
authors.initials: LJ
authors.lastname: Quintans Júnior
mesh_terms: D000818:Animals; D017837:Bothrops; D007887:Fabaceae / Q000737:chemistry; D006801:Humans; D006930:Hyperalgesia / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D018482:Muscle, Skeletal / Q000187:drug effects / Q000293:injuries; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D012909:Snake Bites / Q000188:drug therapy* / Q000473:pathology; D012910:Snake Venoms / Q000633:toxicity
chemical_list: D010936:Plant Extracts; D012910:Snake Venoms