title: Sirtuin 3 interacts with Lon protease and regulates its acetylation status.
abstract: Lon is a mitochondrial protease that degrades oxidized damaged proteins, assists protein folding and participates in maintaining mitochondrial DNA levels. Changes in Lon mRNA levels, protein levels and activity are not always directly correlated, suggesting that Lon could be regulated at post translational level. We found that Lon and SIRT3, the most important mitochondrial sirtuin, colocalize and coimmunoprecipitate in breast cancer cells, and silencing or inhibition of Lon did not alter SIRT3 levels. Silencing of SIRT3 increased the levels of Lon protein and of its acetylation, suggesting that Lon is a target of SIRT3, likely at K917.
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41125 Modena, Italy.
authors.forename: Lara
authors.initials: L
authors.lastname: Gibellini
authors.affiliation: Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41125 Modena, Italy. Electronic address: marcello.pinti@unimore.it.
authors.forename: Marcello
authors.initials: M
authors.lastname: Pinti
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41125 Modena, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Beretti
authors.affiliation: Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Via Orabona 4, 70125 Bari, Italy.
authors.forename: Ciro Leonardo
authors.initials: CL
authors.lastname: Pierri
authors.affiliation: Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Via Orabona 4, 70125 Bari, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Onofrio
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41125 Modena, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Riccio
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41125 Modena, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Carnevale
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41125 Modena, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: De Biasi
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41125 Modena, Italy.
authors.forename: Milena
authors.initials: M
authors.lastname: Nasi
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41125 Modena, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Torelli
authors.affiliation: Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41125 Modena, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Boraldi
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41125 Modena, Italy.
authors.forename: Anto
authors.initials: A
authors.lastname: De Pol
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41125 Modena, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Cossarizza
mesh_terms: D000107:Acetylation; D045744:Cell Line, Tumor; D055785:Gene Knockdown Techniques; D006801:Humans; D047468:Immunoprecipitation; D008928:Mitochondria / Q000737:chemistry; D049070:Protease La / Q000378:metabolism*; D025941:Protein Interaction Mapping; D011499:Protein Processing, Post-Translational; D056566:Sirtuin 3 / Q000378:metabolism*
chemical_list: D049070:Protease La; D056566:Sirtuin 3
keywords: Acetylation; Lon; Mitochondria; SIRT3