title: Glioma-derived ADAM10 induces regulatory B cells to suppress CD8+ T cells.
abstract: CD8+ T cells play an important role in the anti-tumor activities of the body. The dysfunction of CD8+ T cells in glioma is unclear. This study aims to elucidate the glioma cell-derived ADAM10 (A Disintegrin and metalloproteinase domain-containing protein 10) in the suppression of CD8+ effector T cells by the induction of regulatory B cells. In this study, glioma cells were isolated from surgically removed glioma tissue and stimulated by Phorbol myristate acetage (PMA) in the culture. The levels of ADAM10 in the culture were determined by enzyme-linked immunosorbent assay. Immune cells were assessed by flow cytometry. The results showed that the isolated glioma cells express ADAM10, which was markedly up regulated after stimulated with PMA. The glioma-derived ADAM10 induced activated B cells to differentiate into regulatory B cells, the later suppressed CD8+ T cell proliferation as well as the induced regulatory T cells, which also showed the immune suppressor effect on CD8+ effector T cell proliferation. In conclusion, glioma cells produce ADAM10 to induce Bregs; the latter suppresses CD8+ T cells and induces Tregs.
authors.affiliation: Department of neurosurgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Zhuo-peng
authors.initials: ZP
authors.lastname: Ye
authors.affiliation: Department of neurosurgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Hai-yong
authors.initials: HY
authors.lastname: He
authors.affiliation: Department of neurosurgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of neurosurgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Wen-sheng
authors.initials: WS
authors.lastname: Li
authors.affiliation: Department of neurosurgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Lun
authors.initials: L
authors.lastname: Luo
authors.affiliation: Department of neurosurgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Zhen-chao
authors.initials: ZC
authors.lastname: Huang
authors.affiliation: Department of neurosurgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
mesh_terms: D051722:ADAM Proteins / Q000502:physiology*; D000072197:ADAM10 Protein; D053829:Amyloid Precursor Protein Secretases / Q000502:physiology*; D001402:B-Lymphocytes / Q000502:physiology*; D060151:B-Lymphocytes, Regulatory / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005910:Glioma / Q000201:enzymology* / Q000276:immunology; D006801:Humans; D007108:Immune Tolerance; D008213:Lymphocyte Activation; D008565:Membrane Proteins / Q000502:physiology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014407:Tumor Cells, Cultured
chemical_list: D008565:Membrane Proteins; D053829:Amyloid Precursor Protein Secretases; D051722:ADAM Proteins; D000072197:ADAM10 Protein; C491236:ADAM10 protein, human