title: Self-assembly and release of peste des petits ruminants virus-like particles in an insect cell-baculovirus system and their immunogenicity in mice and goats.
abstract: Peste des petits ruminants (PPR) is an acute, febrile, viral disease of small ruminants that has a significant economic impact. For many viral diseases, vaccination with virus-like particles (VLPs) has shown considerable promise as a prophylactic approach; however, the processes of assembly and release of peste des petits ruminants virus (PPRV) VLPs are not well characterized, and their immunogenicity in the host is unknown. In this study, VLPs of PPRV were generated in a baculovirus system through simultaneous expression of PPRV matrix (M) protein and hemaglutin in (H) or fusion (F) protein. The released VLPs showed morphology similar to that of the native virus particles. Subcutaneous injection of these VLPs (PPRV-H, PPRV-F) into mice and goats elicited PPRV-specific IgG production, increased the levels of virus neutralizing antibodies, and promoted lymphocyte proliferation. Without adjuvants, the immune response induced by the PPRV-H VLPs was comparable to that obtained using equivalent amounts of PPRV vaccine. Thus, our results demonstrated that VLPs containing PPRV M protein and H or F protein are potential "differentiating infected from vaccinated animals" (DIVA) vaccine candidates for the surveillance and eradication of PPR.
authors.affiliation: State Key Laboratory of Animal Nutrition, Beijing Institute of Animal Science and Veterinary Medicine, Chinese Academy of Agricultural Sciences; Beijing Scientific Observation and Experiment Station for Veterinary Drug and Veterinary Biotechnology, Ministry of Agriculture, Beijing, PR China; Beijing CevaHuadu Biological Co., Ltd, Beijing, PR China.
authors.forename: Wenchao
authors.initials: W
authors.lastname: Li
authors.affiliation: State Key Laboratory of Animal Nutrition, Beijing Institute of Animal Science and Veterinary Medicine, Chinese Academy of Agricultural Sciences; Beijing Scientific Observation and Experiment Station for Veterinary Drug and Veterinary Biotechnology, Ministry of Agriculture, Beijing, PR China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Animal Nutrition, Beijing Institute of Animal Science and Veterinary Medicine, Chinese Academy of Agricultural Sciences; Beijing Scientific Observation and Experiment Station for Veterinary Drug and Veterinary Biotechnology, Ministry of Agriculture, Beijing, PR China.
authors.forename: Xiukun
authors.initials: X
authors.lastname: Sui
authors.affiliation: State Key Laboratory of Animal Nutrition, Beijing Institute of Animal Science and Veterinary Medicine, Chinese Academy of Agricultural Sciences; Beijing Scientific Observation and Experiment Station for Veterinary Drug and Veterinary Biotechnology, Ministry of Agriculture, Beijing, PR China.
authors.forename: Zhanzhong
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: China Institute of Veterinary Drug Control, Beijing, PR China.
authors.forename: Chenghuai
authors.initials: C
authors.lastname: Yang
authors.affiliation: Beijing CevaHuadu Biological Co., Ltd, Beijing, PR China.
authors.forename: Wenquan
authors.initials: W
authors.lastname: Wang
authors.affiliation: China Institute of Veterinary Drug Control, Beijing, PR China.
authors.forename: Junping
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Animal Nutrition, Beijing Institute of Animal Science and Veterinary Medicine, Chinese Academy of Agricultural Sciences; Beijing Scientific Observation and Experiment Station for Veterinary Drug and Veterinary Biotechnology, Ministry of Agriculture, Beijing, PR China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood* / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D005260:Female; D015511:Goat Diseases / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D006041:Goats / Q000276:immunology / Q000821:virology; D006389:Hemagglutinins, Viral / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D029021:Peste-des-Petits-Ruminants / Q000276:immunology* / Q000517:prevention & control; D018107:Peste-des-petits-ruminants virus / Q000235:genetics / Q000276:immunology*; D011897:Random Allocation; D061987:Sf9 Cells; D018411:Spodoptera; D014611:Vaccination / Q000662:veterinary*; D058425:Vaccines, Virus-Like Particle / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000276:immunology; D014763:Viral Matrix Proteins / Q000276:immunology; D019065:Virus Assembly / Q000502:physiology; D057074:Virus Release / Q000502:physiology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D007074:Immunoglobulin G; D058425:Vaccines, Virus-Like Particle; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins