title: Sono-assisted extraction of alcohol-insoluble extract from Althaea rosea: purification and chemical analysis.
abstract: A Box-Behnken design (BBD) was used to evaluate the effects of ultrasonic power, extraction time, extraction temperature, and water to raw material ratio on extraction yield of alcohol-insoluble polysaccharide of Althaea rosea leaf (ARLP). Purification was carried out by dialysis method. Chemical analysis of ARLP revealed contained 12.69 ± 0.48% moisture, 79.33 ± 0.51% total sugar, 3.82 ± 0.21% protein, 11.25 ± 0.37% uronic acid and 3.77 ± 0.15% ash. The response surface methodology (RSM) showed that the significant quadratic regression equation with high R(2) (=0.9997) was successfully fitted for extraction yield of ARLP as function of independent variables. The overall optimum region was found to be at the combined level of ultrasonic power 91.85 W, extraction time 29.94 min, extraction temperature 89.78 °C, and the ratio of water to raw material 28.77 (mL/g). At this optimum point, extraction yield of ARLP was 19.47 ± 0.41%. No significant (p>0.05) difference was found between the actual and predicted (19.30 ± 0.075%) values. The results demonstrated that ARLP had strong scavenging activities on DPPH and hydroxyl radicals.
authors.affiliation: Department of Food Science and Technology, Collage of Agricultural and Natural resources, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran. Electronic address: meghdad.eskandari68@gmail.com.
authors.forename: Meghdad
authors.initials: M
authors.lastname: Eskandari
authors.affiliation: Department of Food Science and Engineering, Faculty of Agricultural Engineering and Technology, University of Tehran, Iran. Electronic address: vsamavati@ut.ac.ir.
authors.forename: Vahid
authors.initials: V
authors.lastname: Samavati
mesh_terms: D031583:Althaea / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry* / Q000627:therapeutic use; D000431:Ethanol / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000627:therapeutic use; D006801:Humans; D017665:Hydroxyl Radical / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D013696:Temperature; D014465:Ultrasonics; D014867:Water / Q000737:chemistry
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D011134:Polysaccharides; D014867:Water; D017665:Hydroxyl Radical; D000431:Ethanol
keywords: Althaea rosea; Box–Behnken design; Purification; Sono-physic