title: Carbohydrate-binding specificity of lectins using multiplexed glyco-bead array.
abstract: Multiplexed bead array is an application that allows us to quantify multiple ligands simultaneously by using flow cytometry. Glycopeptides are immobilized on multiplexed beads, and the glycan-binding specificities of several lectins are determined. This strategy is easy, rapid, and suitable for small amount of samples, and allows the reliable elucidation under the identical condition. Such a technology is useful for analyzing characteristics and functions of lectins.
authors.affiliation: Laboratory of Molecular Medicine, Department of Integrated Sciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba, 277-8562, Japan, yamamoto@k.u-tokyo.ac.jp.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D002264:Carboxylic Acids / Q000737:chemistry; D005434:Flow Cytometry; D006020:Glycopeptides / Q000737:chemistry / Q000378:metabolism*; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D008024:Ligands; D046228:Microarray Analysis / Q000379:methods*; D008863:Microspheres; D013379:Substrate Specificity; D013552:Swine; D013997:Time Factors
chemical_list: D002264:Carboxylic Acids; D006020:Glycopeptides; D055767:Immobilized Proteins; D037102:Lectins; D008024:Ligands