title: Serum diamine oxidase as a hemorrhagic shock biomarker in a rabbit model.
abstract: BACKGROUND
  In prolonged hemorrhagic shock, reductions in intestinal mucosal blood perfusion lead to mucosal barrier damage and systemic inflammation. Gastrointestinal failure in critically ill patients has a poor prognosis, so early assessment of mucosal barrier injury in shock patients is clinically relevant. Unfortunately, there is no serum marker that can accurately assess intestinal ischemia-reperfusion injury.
  
  
  OBJECTIVE
  The aim of this study was to assess if serum diamine oxidase levels can reflect intestinal mucosal injury subsequent to prolonged hemorrhagic shock.
  
  
  METHODS
  Thirty New Zealand white rabbits were divided into three groups: a control group, a medium blood pressure (BP) group (exsanguinated to a shock BP of 50 to 41 mm Hg), and a low BP group (exsanguinated to a shock blood pressure of 40 to 31 mm Hg), in which the shock BP was sustained for 180 min prior to fluid resuscitation.
  
  
  RESULTS
  The severity of hemorrhagic shock in the low BP group was significantly greater than that of the medium BP group according to the post-resuscitation BP, serum tumor necrosis factor (TNF)-α, and arterial lactate. Intestinal damage was significantly more severe in the low BP group according to Chiu's scoring, claudin-1, intercellular adhesion molecule (ICAM)-1, and myeloperoxidase expression. Serum diamine oxidase was significantly increased in the low BP group compared to the medium BP and control groups and was negatively correlated with shock BP.
  
  
  CONCLUSIONS
  Serum diamine oxidase can be used as a serological marker in evaluating intestinal injury and shows promise as an indicator of hemorrhagic shock severity.
authors.affiliation: Department of Cardiovascular Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Cardiovascular Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Luo
authors.affiliation: Department of Cardiovascular Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Weikun
authors.initials: W
authors.lastname: Jia
authors.affiliation: Department of Cardiovascular Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Department of Cardiovascular Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Huang
authors.affiliation: Department of Cardiovascular Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Geng
authors.initials: G
authors.lastname: Tian
authors.affiliation: Department of Cardiovascular Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Cardiovascular Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Yingbin
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000097:blood*; D000818:Animals; D015415:Biomarkers / Q000097:blood*; D001794:Blood Pressure / Q000502:physiology; D005440:Fluid Therapy; D008297:Male; D011817:Rabbits; D012771:Shock, Hemorrhagic / Q000097:blood* / Q000628:therapy
chemical_list: D015415:Biomarkers; D006631:Amine Oxidase (Copper-Containing)