title: Functional tooth restoration by next-generation bio-hybrid implant as a bio-hybrid artificial organ replacement therapy.
abstract: Bio-hybrid artificial organs are an attractive concept to restore organ function through precise biological cooperation with surrounding tissues in vivo. However, in bio-hybrid artificial organs, an artificial organ with fibrous connective tissues, including muscles, tendons and ligaments, has not been developed. Here, we have enveloped with embryonic dental follicle tissue around a HA-coated dental implant, and transplanted into the lower first molar region of a murine tooth-loss model. We successfully developed a novel fibrous connected tooth implant using a HA-coated dental implant and dental follicle stem cells as a bio-hybrid organ. This bio-hybrid implant restored physiological functions, including bone remodelling, regeneration of severe bone-defect and responsiveness to noxious stimuli, through regeneration with periodontal tissues, such as periodontal ligament and cementum. Thus, this study represents the potential for a next-generation bio-hybrid implant for tooth loss as a future bio-hybrid artificial organ replacement therapy.
authors.affiliation: 1] Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, JAPAN [2] [3].
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Oshima
authors.affiliation: 1] Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, JAPAN [2] Division of Orthodontics and Dentofacial Orthopedics, Graduate School of Dentistry, Tohoku University, Sendai, Miyagi, 980-8575, JAPAN [3].
authors.forename: Kaoru
authors.initials: K
authors.lastname: Inoue
authors.affiliation: 1] Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, JAPAN [2] Department of Clinical Pathophysiology, Tokyo Dental College, Chiba-shi, Chiba, 261-8502, JAPAN.
authors.forename: Kei
authors.initials: K
authors.lastname: Nakajima
authors.affiliation: Department of Oral Pathology, Showa University School of Dentistry, Shinagawa-ku, Tokyo, 145-8515, JAPAN.
authors.forename: Tetsuhiko
authors.initials: T
authors.lastname: Tachikawa
authors.affiliation: Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, JAPAN.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Yamazaki
authors.affiliation: Department of Oral Pathology, Showa University School of Dentistry, Shinagawa-ku, Tokyo, 145-8515, JAPAN.
authors.forename: Tomohide
authors.initials: T
authors.lastname: Isobe
authors.affiliation: Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, JAPAN.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Sugawara
authors.affiliation: 1] Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, JAPAN [2] Organ Technologies Inc., Tokyo, 108-0074, JAPAN.
authors.forename: Miho
authors.initials: M
authors.lastname: Ogawa
authors.affiliation: Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, JAPAN.
authors.forename: Chie
authors.initials: C
authors.lastname: Tanaka
authors.affiliation: Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, JAPAN.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Saito
authors.affiliation: Section of Oral Implantology and Regenerative Dental Medicine, Graduate School of Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8549, JAPAN.
authors.forename: Shohei
authors.initials: S
authors.lastname: Kasugai
authors.affiliation: Division of Orthodontics and Dentofacial Orthopedics, Graduate School of Dentistry, Tohoku University, Sendai, Miyagi, 980-8575, JAPAN.
authors.forename: Teruko
authors.initials: T
authors.lastname: Takano-Yamamoto
authors.affiliation: Department of Clinical Pathophysiology, Tokyo Dental College, Chiba-shi, Chiba, 261-8502, JAPAN.
authors.forename: Takashi
authors.initials: T
authors.lastname: Inoue
authors.affiliation: 1] Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, JAPAN [2] Organ Technologies Inc., Tokyo, 108-0074, JAPAN.
authors.forename: Katsunari
authors.initials: K
authors.lastname: Tezuka
authors.affiliation: Department of Oral Rehabilitation and Regenerative Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8525, JAPAN.
authors.forename: Takuo
authors.initials: T
authors.lastname: Kuboki
authors.affiliation: Section of Oral Pathology, Department of Oral Restitution, Graduate School of Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8549, JAPAN.
authors.forename: Akira
authors.initials: A
authors.lastname: Yamaguchi
authors.affiliation: 1] Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, JAPAN [2] Department of Biological Science and Technology, Graduate School of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, JAPAN [3] Organ Technologies Inc., Tokyo, 108-0074, JAPAN [4].
authors.forename: Takashi
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D001187:Artificial Organs; D001672:Biocompatible Materials; D001861:Bone Regeneration; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D003739:Dental Cementum / Q000378:metabolism; D015921:Dental Implants; D003795:Dental Sac / Q000166:cytology / Q000502:physiology; D017886:Durapatite / Q000737:chemistry; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D009971:Orthodontics, Corrective / Q000379:methods*; D015675:Osteocalcin / Q000096:biosynthesis; D010513:Periodontal Ligament / Q000502:physiology / Q000601:surgery; D023822:Tissue Engineering / Q000379:methods*; D014070:Tooth / Q000601:surgery / Q000637:transplantation*
chemical_list: D001672:Biocompatible Materials; D015815:Cell Adhesion Molecules; D015921:Dental Implants; D016326:Extracellular Matrix Proteins; C552523:F-spondin protein, mouse; C082838:Postn protein, mouse; D015675:Osteocalcin; D017886:Durapatite