title: Quantification of V(D)J recombination by real-time quantitative PCR.
abstract: B and T lymphocytes have the unique capacity to somatically rearrange their antigen receptor loci through V(D)J recombination. D-JH and VH-DJH recombination events are usually visualized by semi-quantitative PCR followed by detection of end products, which is time consuming and requires the use of hazardous elements. Additionally, it necessitates relatively large amounts of genomic DNA which could be limiting when the cell populations of interest are rare. Here, we describe a real-time quantitative PCR assay for a fast quantification of V(D)J recombination events at the IgH locus.
authors.affiliation: CNRS UMR 5089; IPBS (Institut de Pharmacologie et de Biologie Structurale); FRBT, 205 route de Narbonne, BP 64182, F-31077 Toulouse, France; Université de Toulouse; UPS; IPBS F-31077 Toulouse, France.
authors.forename: Fatima-Zohra
authors.initials: FZ
authors.lastname: Braikia
authors.affiliation: CNRS UMR 5089; IPBS (Institut de Pharmacologie et de Biologie Structurale); FRBT, 205 route de Narbonne, BP 64182, F-31077 Toulouse, France; Université de Toulouse; UPS; IPBS F-31077 Toulouse, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Chemin
authors.affiliation: CNRS UMR 5089; IPBS (Institut de Pharmacologie et de Biologie Structurale); FRBT, 205 route de Narbonne, BP 64182, F-31077 Toulouse, France; Université de Toulouse; UPS; IPBS F-31077 Toulouse, France.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Moutahir
authors.affiliation: CNRS UMR 5089; IPBS (Institut de Pharmacologie et de Biologie Structurale); FRBT, 205 route de Narbonne, BP 64182, F-31077 Toulouse, France; Université de Toulouse; UPS; IPBS F-31077 Toulouse, France. Electronic address: ahmed.khamlichi@ipbs.fr.
authors.forename: Ahmed Amine
authors.initials: AA
authors.lastname: Khamlichi
mesh_terms: D000818:Animals; D023061:Gene Order; D050438:Genes, Immunoglobulin Heavy Chain; D056426:Genetic Loci; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction / Q000592:standards; D060152:V(D)J Recombination
keywords: B lymphocyte; IgH locus; Real-time quantitative PCR; V(D)J recombination