title: Remodeling cell surface glycans using glycosylation inhibitors.
abstract: Cell surface glycan remodeling is a useful method to modulate glycan-lectin interactions. In this chapter, a facile and reliable method to remodel mammalian cell surface N-glycans using inhibitors for N-glycan-processing enzymes is described. The method is widely applicable to many mammalian systems because those inhibitors work for the conserved glycosylation pathways among species.
authors.affiliation: Food & Health Programme, Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, UK, norihito.kawasaki@ifr.ac.uk.
authors.forename: Norihito
authors.initials: N
authors.lastname: Kawasaki
mesh_terms: D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005434:Flow Cytometry; D006031:Glycosylation / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D037121:Plant Lectins / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: D004791:Enzyme Inhibitors; D037121:Plant Lectins; D011134:Polysaccharides