title: Free-radical degradation by Fe2+/Vc/H2O2 and antioxidant activity of polysaccharide from Tremella fuciformis.
abstract: The free-radical degradation and antioxidant activity of polysaccharide from Tremella fuciformis was investigated. In present study, the combination of Fe(2+), ascorbic acid and H2O2 was used as degradation regents in order to obtain the lower molecular weight product. The result ascertained oxidative-reduce degradation did not change the main structure of polysaccharides in the test conditions. Five degraded polysaccharides were selected to evaluate their antioxidant activities in vitro. It was found that the degraded sample with lower molecular weight possessed the higher antioxidant activities. It was possible that free-radical degradation is an effective way for enhancing antioxidant activity to decrease molecular weight of polysaccharides.
authors.affiliation: Department of Pharmacology, Huzhou Teachers College, Huzhou 313000, China. Electronic address: zhzs@hutc.zj.cn.
authors.forename: Zhongshan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, Huzhou Teachers College, Huzhou 313000, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Huzhou Teachers College, Huzhou 313000, China.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Zhao
authors.affiliation: School of Pharmaceutical and Biological Sciences, Weifang Medical University, Weifang 261053, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Qi
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000737:chemistry; D001487:Basidiomycota / Q000737:chemistry*; D005609:Free Radicals / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013329:Structure-Activity Relationship
chemical_list: D000975:Antioxidants; C045076:Fenton's reagent; D005609:Free Radicals; D011134:Polysaccharides; D006861:Hydrogen Peroxide; D007501:Iron; D001205:Ascorbic Acid
keywords: Antioxidant activity; Degradation; Polysaccharide; Tremella fuciformis