title: Gr-Pt hybrid NP modified GCPE as label and indicator free electrochemical genosensor platform.
abstract: Glassy carbon paste electrode (GCPE) was modified with graphene platinum hybrid nanoparticle (Gr-Pt hybrid NP) and used as a transducer for label and indicator free electrochemical genosensor. 22 mer oligonucleotides representing Escherichia coli bacteria were used as a model case. As far as it is known, this study is the first study where Gr-Pt hybrid NP was incorporated into GCPE and used for genosensor transducer. The extent of hybridization was determined by using differential pulse voltammetric signals of guanin oxidation. After the optimization of experimental parameters, analytical characteristics were investigated. The linear range was found between 1.5×10(-7) and 2.25×10(-6) M with the equation of y=1.6566x-2.6161 and R(2) of 0.9959. RSD and LOD were calculated as 4.2% (n=6) and 1.12×10(-9) M respectively.
authors.affiliation: Muğla Sıtkı Koçman University, Faculty of Science, Chemistry Department, 48000 Kötekli, Muğla Turkey.
authors.forename: Sinan Cemgil
authors.initials: SC
authors.lastname: Sultan
authors.affiliation: Muğla Sıtkı Koçman University, Faculty of Science, Chemistry Department, 48000 Kötekli, Muğla Turkey. Electronic address: ulkuanik@yahoo.com.
authors.forename: Ülkü
authors.initials: Ü
authors.lastname: Anik
mesh_terms: D004563:Electrochemistry / Q000379:methods*; D004926:Escherichia coli / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006147:Guanine / Q000737:chemistry; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D010984:Platinum / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D010984:Platinum; D006147:Guanine; D006108:Graphite; D010100:Oxygen
keywords: E. coli; GCPE; Genosensor; Graphene–platinum hybrid nanoparticle