title: Simultaneous determination of bambuterol and its two major metabolites in human plasma by hydrophilic interaction ultra-performance liquid chromatography-tandem mass spectrometry.
abstract: In this study, a rapid and sensitive hydrophilic interaction ultra-performance liquid chromatography-tandem mass spectrometry (HILIC-UPLC-MS/MS) method was developed for simultaneous determination of bambuterol and its two major metabolites monocarbamate bambuterol and terbutaline in human plasma. All samples were simply precipitated using acetonitrile and separated on a UPLC-HILIC column under gradient elution with a mobile phase consisting of acetonitrile and water with the addition of 10mm ammonium acetate and 0.1% formic acid at 0.4 mL/min. The analytes were detected by a Xevo TQ-S tandem mass spectrometer with positive electrospray ionization in multiple reaction monitoring mode. The established method was highly sensitive with the lower limit of quantification (LLOQ) of 10.00 pg/mL for each analyte, and the intra- and inter-day precisions were <12.8%. The analytical runtime within 4.0 min per sample made this method suitable for high throughput determination. The validated method was successfully applied to a clinical pharmacokinetic study of bambuterol in eight healthy volunteers. Furthermore, the effects of the chromatographic conditions on the retention of the analytes on HILIC were investigated, and the benefits of HILIC were evaluated by comparing with a C18 column. The results indicated that liquid-liquid partition and the electrostatic interactions played an important role in the retention of the analytes on HILIC in this study. And HILIC offered particular advantages over RPLC approach in the aspects of the peak symmetry, the column efficiency, and the column pressure.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China; South China University of Technology-Waters Technology Joint Laboratory, South China University of Technology, Guangzhou, 510006, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhou
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China; South China University of Technology-Waters Technology Joint Laboratory, South China University of Technology, Guangzhou, 510006, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Cheng
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China; South China University of Technology-Waters Technology Joint Laboratory, South China University of Technology, Guangzhou, 510006, China.
authors.forename: Chengjuan
authors.initials: C
authors.lastname: Zou
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China; South China University of Technology-Waters Technology Joint Laboratory, South China University of Technology, Guangzhou, 510006, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhao
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China; South China University of Technology-Waters Technology Joint Laboratory, South China University of Technology, Guangzhou, 510006, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Liu
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China; South China University of Technology-Waters Technology Joint Laboratory, South China University of Technology, Guangzhou, 510006, China.
authors.forename: Marco
authors.initials: M
authors.lastname: Pistolozzi
authors.affiliation: Keypharma Biomedical Inc., Songshan Lake Science & Technology Industry Park, Dongguan, 523808, China.
authors.forename: Evina
authors.initials: E
authors.lastname: Tan
authors.affiliation: Keypharma Biomedical Inc., Songshan Lake Science & Technology Industry Park, Dongguan, 523808, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Xu
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China; South China University of Technology-Waters Technology Joint Laboratory, South China University of Technology, Guangzhou, 510006, China. Electronic address: went@scut.edu.cn.
authors.forename: Wen
authors.initials: W
authors.lastname: Tan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D016014:Linear Models; D011312:Pressure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013696:Temperature; D013726:Terbutaline / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics
chemical_list: D013726:Terbutaline; C047766:bambuterol
keywords: Bambuterol; HILIC-UPLC-MS/MS; Major metabolites; Pharmacokinetics.; Plasma