title: Surface-initiated hyperbranched polyglycerol as an ultralow-fouling coating on glass, silicon, and porous silicon substrates.
abstract: Anionic ring-opening polymerization of glycidol was initiated from activated glass, silicon, and porous silicon substrates to yield thin, ultralow-fouling hyperbranched polyglycerol (HPG) graft polymer coatings. Substrates were activated by deprotonation of surface-bound silanol functionalities. HPG polymerization was initiated upon the addition of freshly distilled glycidol to yield films in the nanometer thickness range. X-ray photoelectron spectroscopy, contact angle measurements, and ellipsometry were used to characterize the resulting coatings. The antifouling properties of HPG-coated surfaces were evaluated in terms of protein adsorption and the attachment of mammalian cells. The adsorption of bovine serum albumin and collagen type I was found to be reduced by as much as 97 and 91%, respectively, in comparison to untreated surfaces. Human glioblastoma and mouse fibroblast attachment was reduced by 99 and 98%, respectively. HPG-grafted substrates outperformed polyethylene glycol (PEG) grafted substrates of comparable thickness under the same incubation conditions. Our results demonstrate the effectiveness of antifouling HPG graft polymer coatings on a selected range of substrate materials and open the door for their use in biomedical applications.
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Mawson Institute, University of South Australia , GPO Box 2471, Adelaide, South Australia 5001, Australia.
authors.forename: Eli
authors.initials: E
authors.lastname: Moore
authors.forename: Bahman
authors.initials: B
authors.lastname: Delalat
authors.forename: Roshan
authors.initials: R
authors.lastname: Vasani
authors.forename: Gordon
authors.initials: G
authors.lastname: McPhee
authors.forename: Helmut
authors.initials: H
authors.lastname: Thissen
authors.forename: Nicolas H
authors.initials: NH
authors.lastname: Voelcker
mesh_terms: D000818:Animals; D057219:Biofouling; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D024042:Collagen Type I / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005898:Glass / Q000737:chemistry*; D005909:Glioblastoma / Q000473:pathology; D005990:Glycerol / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D010084:Oxidation-Reduction / Q000187:drug effects; D056951:Photoelectron Spectroscopy; D058105:Polymerization / Q000187:drug effects; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology*; D016062:Porosity; D012710:Serum Albumin, Bovine / Q000378:metabolism; D012825:Silicon / Q000494:pharmacology*; D013499:Surface Properties
chemical_list: D024042:Collagen Type I; D011108:Polymers; C043941:polyglycerol; D012710:Serum Albumin, Bovine; D005990:Glycerol; D012825:Silicon
keywords: antifouling; biofouling; hyperbranched polyglycerol; low-fouling; nonfouling; surface grafting