title: Label free detection of specific protein binding using a microwave sensor.
abstract: The specific binding of streptavidin to biotinylated protein A was demonstrated using a microwave detection system. In control experiments, the degree of non-specific binding was negligible. The method of detection was used to monitor the adsorption of two other proteins, cytochrome c and glucose oxidase, on to the IDE microwave sensor surface. The response of the sensor was also examined on different substrate materials, with detection of protein binding observed obtained on both smooth, conductive (gold) and on rough, insulating (hydroxyapatite) surfaces.
authors.affiliation: Department of Chemical and Environmental Science & Materials and Surface Science Institute, University of Limerick, Limerick, Ireland. edmond.magner@ul.ie.
authors.forename: Marcela
authors.initials: M
authors.lastname: Salazar-Alvarez
authors.forename: Olga
authors.initials: O
authors.lastname: Korostynska
authors.forename: Alex
authors.initials: A
authors.lastname: Mason
authors.forename: Ahmed
authors.initials: A
authors.lastname: Al-Shamma'a
authors.forename: Jakki C
authors.initials: JC
authors.lastname: Cooney
authors.forename: Edmond
authors.initials: E
authors.lastname: Magner
authors.forename: Syed A M
authors.initials: SA
authors.lastname: Tofail
mesh_terms: D008855:Microscopy, Electron, Scanning; D008872:Microwaves; D011485:Protein Binding; D013205:Staphylococcal Protein A / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry*
chemical_list: D013205:Staphylococcal Protein A; D019809:Streptavidin