title: Agar-supported cultivation of Halorubrum sp. SSR, and production of halocin C8 on the scale-up prototype Platotex.
abstract: Halorubrum sp. SSR was isolated from a solar saltern in Algeria. The strain exhibited a high antibiotic activity against the indicator strain Natronorubrum aibiense G23, and the bioactive compound showed thermal, acid and alkali stability. SSR was grown on agar-supported cultivation (AgSF) to compare yields and applicability with traditional submerged cultivation. AgSF scale-up was implemented taking benefit from the solid-state cultivation prototype Platotex. This technology leads to high amounts of the target Halocin and facilitate the downstream steps. The antibiotic compound was purified according to a fast efficient procedure including ion exchange chromatography followed by a fractionation on C18 Sep-Pack cartridge. The compound was identified as Halocin C8 according to N-terminal amino acid sequencing and high-resolution mass spectrometry.
authors.affiliation: Faculty of Biological Sciences, Cellular and Molecular Biology Laboratory, University of Sciences and Technology Houari Boumediene, Bp 32, El Alia, 16111, Bab Ezzouar Algiers, Algeria.
authors.forename: Rima
authors.initials: R
authors.lastname: Meknaci
authors.forename: Philippe
authors.initials: P
authors.lastname: Lopes
authors.forename: Claudine
authors.initials: C
authors.lastname: Servy
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Le Caer
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Andrieu
authors.forename: Hocine
authors.initials: H
authors.lastname: Hacène
authors.forename: Jamal
authors.initials: J
authors.lastname: Ouazzani
mesh_terms: D000362:Agar / Q000032:analysis; D023181:Antimicrobial Cationic Peptides; D019149:Bioreactors; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D055352:Halorubrum / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D007218:Industrial Microbiology / Q000295:instrumentation / Q000379:methods*; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism
chemical_list: D023181:Antimicrobial Cationic Peptides; D003470:Culture Media; D010455:Peptides; C413338:halocin S8; D000362:Agar