title: [Purification of antigenic fraction 27-28 kDa from the metabolic antigen from metabolic secreted-excreted from Fasciola hepatica].
abstract: Antigenic fractions of 27-28 kDa from Fasciola hepatica were purified by size-exclusion chromatography for use in the diagnosis of human fasciolosis. Excretion and secretion antigens were obtained from living adult flukes collected from sheep and cattle liver, and cultured in minimum essential medium. The reactivity of the purified antigen and efficacy were assessed by immunoblot test using four sera with human fascioliasis; four sera with other parasites, and two negative sera. We conclude that the purified antigenic fractions do not cross-react with other parasites by immunoblot. Therefore, purified proteins are considered as potential candidates to be used for the diagnosis of human fascioliasis.
authors.affiliation: Laboratorio de Zoonosis Parasitaria, Instituto Nacional de Salud, Lima, Perú
authors.forename: Isidro
authors.initials: I
authors.lastname: Antitupa
authors.affiliation: Laboratorio de Zoonosis Parasitaria, Instituto Nacional de Salud, Lima, Perú
authors.forename: William
authors.initials: W
authors.lastname: Quispe
authors.affiliation: Laboratorio de Zoonosis Parasitaria, Instituto Nacional de Salud, Lima, Perú
authors.forename: Jhon
authors.initials: J
authors.lastname: Mayo
authors.affiliation: Laboratorio de Zoonosis Parasitaria, Instituto Nacional de Salud, Lima, Perú
authors.forename: Fanny
authors.initials: F
authors.lastname: Valverde
authors.affiliation: Laboratorio de Zoonosis Parasitaria, Instituto Nacional de Salud, Lima, Perú
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Sanchez
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000302:isolation & purification* / Q000378:metabolism; D005210:Fasciola hepatica / Q000276:immunology* / Q000378:metabolism; D005211:Fascioliasis / Q000175:diagnosis; D006801:Humans; D008970:Molecular Weight
chemical_list: D000947:Antigens, Helminth