title: Kisspeptin-10 potentiates miniature excitatory postsynaptic currents in the rat supraoptic nucleus.
abstract: Kisspeptin is the natural ligand of the G protein-coupled receptor -54 and plays a major role in gonadotropin-releasing hormone secretion in the hypothalamus. Kisspeptin-10 is an endogenous derivative of kisspeptin and has 10 -amino acids. Previous studies have demonstrated that central administration of kisspeptin-10 stimulates the secretion of arginine vasopressin (AVP) in male rats. We examined the effects of kisspeptin-10 on- excitatory synaptic inputs to magnocellular neurosecretory cells (MNCs) including AVP neurons in the supraoptic nucleus (SON) by obtaining in vitro whole-cell patch-clamp recordings from slice preparations of the rat brain. The application of kisspeptin-10 (100 nM-1 μM) significantly increased the frequency of miniature excitatory postsynaptic currents (mEPSCs) in a dose-related manner without affecting the amplitude. The kisspeptin-10-induced potentiation of the mEPSCs was significantly attenuated by previous exposure to the kisspeptin receptor antagonist kisspeptin-234 (100 nM) and to the protein kinase C inhibitor bisindolylmaleimide I (20 nM). These results suggest that kisspeptin-10 participates in the regulation of synaptic inputs to the MNCs in the SON by interacting with the kisspeptin receptor.
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan; Division of Cancer Pathophysiology, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan; Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, 3311-1 Yakishiji, Shimotsuke, Tochigi 329-0483, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Yokoyama
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan; Division of Cancer Pathophysiology, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan; Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, 3311-1 Yakishiji, Shimotsuke, Tochigi 329-0483, Japan.
authors.forename: Kouichiro
authors.initials: K
authors.lastname: Minami
authors.affiliation: Division of Cancer Pathophysiology, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan; Tsumura Research Laboratories, Tsumura and Company, 3586 Yoshiwara, Ami-machi, Inashiki-gun, Ibaraki 300-1192, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Terawaki
authors.affiliation: Division of Cancer Pathophysiology, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Miyano
authors.affiliation: Division of Cancer Pathophysiology, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan; Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, 3311-1 Yakishiji, Shimotsuke, Tochigi 329-0483, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Ogata
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Maruyama
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, 3311-1 Yakishiji, Shimotsuke, Tochigi 329-0483, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Takeuchi
authors.affiliation: Division of Cancer Pathophysiology, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan.
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Uezono
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan. Electronic address: yoichi@med.uoeh-u.ac.jp.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Ueta
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000235:genetics / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007211:Indoles / Q000494:pharmacology; D053444:Inhibitory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D059648:Kisspeptins / Q000378:metabolism*; D008297:Male; D008301:Maleimides / Q000494:pharmacology; D055369:Miniature Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D055647:Rats, Transgenic; D017208:Rats, Wistar; D013495:Supraoptic Nucleus / Q000187:drug effects / Q000502:physiology*; D046509:Tissue Culture Techniques
chemical_list: D004791:Enzyme Inhibitors; D007211:Indoles; C491406:Kiss1 protein, rat; D059648:Kisspeptins; D008301:Maleimides; C485184:enhanced green fluorescent protein; D001127:Arginine Vasopressin; D049452:Green Fluorescent Proteins; D011493:Protein Kinase C; C070515:bisindolylmaleimide I
keywords: EPSC; IPSC; Kisspeptin; Slice patch clamp; Supraoptic nucleus