title: CD31(+) cell transplantation promotes recovery from peripheral neuropathy.
abstract: Recently, we reported that human peripheral blood (PB)-derived CD31(+) cells are highly angiogenic. In this study, we investigated the beneficial effects of CD31(+) cells on peripheral neuropathy in mice. CD31(+) cells were collected from the peripheral blood using magnetic activated cell sorting. CD31(+) cells exhibited higher levels of expression of angiogenic genes on real-time reverse transcriptase polymerase chain reaction. Peripheral neuropathy was induced by crushing the sciatic nerve with a hemostat, and CD31(+) cells were then injected intramuscularly along the sciatic nerve. CD31(+) cell transplantation restored motor nerve conduction velocity and voltage amplitude and improved motor coordination. In addition, CD31(+) cell transplantation significantly improved blood perfusion and increased intraneural vascularity in the sciatic nerve. Whole-mount fluorescent imaging and dot blot analysis showed that CD31(+) cells in the nerve possessed high engraftment and anti-apoptotic properties. Additionally, injected CD31(+) cells displayed neurovascular tropism and are highly incorporated with vasculature. Angiogenic cytokines were augmented in CD31(+)-injected nerve tissue, suggesting increased neovascularization. Taken together, these results indicate that CD31(+) cells might be a novel therapeutic strategy in the treatment of peripheral neuropathy.
authors.affiliation: Department of Neurology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: YongNan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Neurology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: ZhuoBo
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Institute of Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung, Republic of Korea; International St. Mary's Hospital, Incheon, Republic of Korea.
authors.forename: Han-Soo
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Family Medicine, College of Medicine, Dong-A University, Busan, Republic of Korea. Electronic address: drhans@dau.ac.kr.
authors.forename: Seongho
authors.initials: S
authors.lastname: Han
authors.affiliation: Institute of Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung, Republic of Korea; International St. Mary's Hospital, Incheon, Republic of Korea. Electronic address: sungwhk@yahoo.co.kr.
authors.forename: Sung-Whan
authors.initials: SW
authors.lastname: Kim
mesh_terms: D000818:Animals; D017690:Cell Transplantation / Q000379:methods; D064987:Cell- and Tissue-Based Therapy; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D016513:Mice, SCID; D018919:Neovascularization, Physiologic / Q000502:physiology; D010523:Peripheral Nervous System Diseases / Q000628:therapy*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000276:immunology / Q000378:metabolism; D012584:Sciatic Nerve / Q000293:injuries / Q000378:metabolism*
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1
keywords: Angiogenesis; CD31; Cell therapy; Engraftment; Peripheral nerve