title: The protective effect of Agaricus blazei Murrill, submerged culture using the optimized medium composition, on alcohol-induced liver injury.
abstract: Agaricus blazei Murrill (ABM), an edible mushroom native to Brazil, is widely used for nonprescript and medicinal purposes. Alcohol liver disease (ALD) is considered as a leading cause for a liver injury in modern dietary life, which can be developed by a prolonged or large intake of alcohol. In this study, the medium composition of ABM was optimized using response surface methodology for maximum mycelial biomass and extracellular polysaccharide (EPS) production. The model predicts to gain a maximal mycelial biomass and extracellular polysaccharide at 1.047 g/100 mL, and 0.367 g/100 mL, respectively, when the potato is 29.88 g/100 mL, the glucose is 1.01 g/100 mL, and the bran is 1.02 g/100 mL. The verified experiments showed that the model was significantly consistent with the model prediction and that the trends of mycelial biomass and extracellular polysaccharide were predicted by artificial neural network. After that, the optimized medium was used for the submerged culture of ABM. Then, alcohol-induced liver injury in mice model was used to examine the protective effect of ABM cultured using the optimized medium on the liver. And the hepatic histopathological observations showed that ABM had a relatively significant role in mice model, which had alcoholic liver damage.
authors.affiliation: School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
authors.forename: Chunchao
authors.initials: C
authors.lastname: Han
authors.affiliation: Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Li
mesh_terms: D000364:Agaricus / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology*; D003470:Culture Media / Q000378:metabolism; D004195:Disease Models, Animal; D000431:Ethanol / Q000009:adverse effects; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051379:Mice; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D012107:Research Design
chemical_list: D003470:Culture Media; D020011:Protective Agents; D000431:Ethanol