title: The detection of a de novo allele of the Glu-1Dx gene in wheat-rye hybrid offspring.
abstract: This study provides a link between a de novo gene and novel phenotype in wheat-rye hybrids that can be used as a model for induced de novo genetic variation. Wide hybridization can produce de novo DNA variation that may cause novel phenotypes. However, there is still a lack of specific links between changed genes and novel phenotypes in wide hybrids. The well-studied high-molecular-weight glutenin subunit (HMW-GS) genes in tribe Triticeae provide a useful model for addressing this issue. In this study, we investigated the feasibility of a wheat-rye hybridization method for inducing de novo phenotypes using the Glu-1Dx2.2 subunit as an example. We developed three hexaploid wheat lines with normal fertility and a Glu-1Dx2.2 variant, named Glu-1Dx2.2 (v) , derived from three F1 hybrids. The wild-type Glu-1Dx2.2 has two direct repeats of 295 bp length separated by an intervening 101 bp in its central repetitive region. In the mutant Glu-1Dx2.2 (v) , one copy of the repeats and the intervening sequence were deleted, probably through homology-dependent illegitimate recombination (IR). This study provides a direct link between a de novo allele and novel phenotype. Our results indicate that the wheat-rye method may be a useful tool to induce de novo genetic variations that broaden the genetic diversity for wheat improvement.
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Yuan
authors.forename: Miao
authors.initials: M
authors.lastname: Liu
authors.forename: Yuyuan
authors.initials: Y
authors.lastname: Ouyang
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: Zeng
authors.forename: Ming
authors.initials: M
authors.lastname: Hao
authors.forename: Lianquan
authors.initials: L
authors.lastname: Zhang
authors.forename: Shunzong
authors.initials: S
authors.lastname: Ning
authors.forename: Zehong
authors.initials: Z
authors.lastname: Yan
authors.forename: Dengcai
authors.initials: D
authors.lastname: Liu
mesh_terms: D000483:Alleles; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D005983:Glutens / Q000235:genetics*; D006824:Hybridization, Genetic; D010641:Phenotype; D012434:Secale / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005983:Glutens; C037302:glutenin