title: Silencing B7-H1 enhances the anti-tumor effect of bladder cancer antigen-loaded dendritic cell vaccine in vitro.
abstract: OBJECTIVE
  The aim of this study was to examine whether short hairpin RNA (shRNA) expressing lentiviral particles targeting B7-H1 infection could result in B7-H1 knockdown on dendritic cells (DCs) and to investigate whether B7-H1 silencing could augment the immune function of DCs and further elicit a more potent anti-tumor immune effect against bladder cancer cells in vitro.
  
  
  METHODS
  Monocyte-derived DCs, which were generated from peripheral blood mononuclear cells, were infected by a recombinant lentivirus containing shRNA sequence aimed at B7-H1. After that, the infected DCs were pulsed by tumor antigens and used to stimulate cytotoxic T lymphocytes-based anti-tumor effect in vitro.
  
  
  RESULTS
  The lentivirus-mediated shRNA delivery method efficiently and effectively silenced B7-H1 in DCs. Furthermore, the B7-H1 silencing enhanced the stimulatory capacity and the secretion of interleukin-12, but down-regulated interleukin-10 secretion. And more importantly, the anti-tumor effect of bladder cancer antigen-loaded DC vaccine in vitro was also potentially augmented.
  
  
  CONCLUSIONS
  This study suggests that a combination of B7-H1 knockdown and target antigen delivery could augment anti-tumor effects in vitro, which potentially provides a novel strategy in the immunotherapy of bladder cancer.
authors.affiliation: Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, People's Republic of China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, People's Republic of China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, The Affiliated Hospital of Qingdao University, Qingdao, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, People's Republic of China.
authors.forename: Shixiu
authors.initials: S
authors.lastname: Shao
authors.affiliation: Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, People's Republic of China.
authors.forename: Xianjun
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, People's Republic of China.
authors.forename: Jiannan
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, People's Republic of China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Niu
authors.affiliation: Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, People's Republic of China.
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Wang
keywords: B7-H1; bladder cancer; dendritic cell; immunotherapy; vaccine