title: The actin-bundling protein TRIOBP-4 and -5 promotes the motility of pancreatic cancer cells.
abstract: TRIOBP isoforms 4 and 5 (TRIOBP-4/-5) are an actin-bundling protein associated with hearing loss. Here, we showed that TRIOBP-4/-5 was up-regulated in human pancreatic carcinoma cells. Knockdown of TRIOBP-4/-5 led to a loss of filopodia and a decrease in cell motility. Confocal microscopy showed that re-expression of GFP-TRIOBP-4 or -5 restored the filopodial formation in TRIOBP-4/-5-deficient PANC-1 cells. Finally, TRIOBP-4/-5 was shown to be overexpressed in human pancreatic cancer tissues. These results demonstrate a novel role of TRIOBP-4/-5 that promotes the motility of pancreatic cancer cells via regulating actin cytoskeleton reorganization in the filopodia of the cells.
authors.affiliation: Department of Physics and Astronomy, Wayne State University, Detroit, MI 48201, USA.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Bao
authors.affiliation: Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Physics and Astronomy, Wayne State University, Detroit, MI 48201, USA.
authors.forename: Laura K
authors.initials: LK
authors.lastname: Gunther
authors.affiliation: Department of Otolaryngology - Head and Neck Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Kitajiri
authors.affiliation: Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: Chunying
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Physics and Astronomy, Wayne State University, Detroit, MI 48201, USA. Electronic address: ee4243@wayne.edu.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sakamoto
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D015153:Blotting, Western; D021441:Carcinoma, Pancreatic Ductal / Q000378:metabolism / Q000473:pathology*; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D008840:Microfilament Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal; D010179:Pancreas / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D011554:Pseudopodia / Q000378:metabolism / Q000473:pathology*; D034741:RNA, Small Interfering / Q000235:genetics; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D014945:Wound Healing
chemical_list: D008840:Microfilament Proteins; D034741:RNA, Small Interfering; C425471:TRIOBP protein, human
keywords: Actin cytoskeleton reorganization; Filopodia; Migration; Pancreatic cancer cells; TRIOBP-4/-5