title: Association between mir-24 and mir-378 in formalin-fixed paraffin-embedded tissues of breast cancer.
abstract: BACKGROUND
  MiR-24/378 is thought to be onco-miRNAs for their ability of enhancing tumor growth. The objective of this study was to evaluate the potential predictive value of miR-24/378 expression in formalin-fixed paraffin-embedded tissues of breast cancer patients.
  
  
  METHODS
  The expression of miR-24/378 was examined in 101 breast cancer patients and 40 controls using real-time quantitative PCR. All statistical analyses were performed using SPSS16.0.
  
  
  RESULTS
  We found that miR-24 and miR-378 were significantly up-regulated in breast cancer patients compared with controls (all P < 0.01). The expression levels of the two miRNAs were highly correlated with each other in breast cancer patients, with r = 0.778 between miR-24 and miR-378. Moreover, the two miRNAs exhibited great capability of discriminating between cancer patients and controls by ROC analysis. MiR-24 and miR-378 showed 0.79 and 0.807 AUC values respectively.
  
  
  CONCLUSIONS
  Over-expression of miR-24 and miR-378 in FFPE tissue of breast cancer patients might conduct as an ideal source for biomarker discovery and validation in breast cancer patients.
authors.affiliation: Affiliated People's Hospital of Jiangsu University Zhenjiang, Jiangsu, China.
authors.forename: Jia-Yu
authors.initials: JY
authors.lastname: Yin
authors.affiliation: Affiliated People's Hospital of Jiangsu University Zhenjiang, Jiangsu, China.
authors.forename: Zhao-Qun
authors.initials: ZQ
authors.lastname: Deng
authors.affiliation: Department of Nutrition, School of Public Health, Sun Yat-Sen University China.
authors.forename: Feng-Qiong
authors.initials: FQ
authors.lastname: Liu
authors.affiliation: Affiliated People's Hospital of Jiangsu University Zhenjiang, Jiangsu, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.affiliation: Affiliated People's Hospital of Jiangsu University Zhenjiang, Jiangsu, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Lin
authors.affiliation: Affiliated People's Hospital of Jiangsu University Zhenjiang, Jiangsu, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Affiliated People's Hospital of Jiangsu University Zhenjiang, Jiangsu, China.
authors.forename: Xiang-Mei
authors.initials: XM
authors.lastname: Wen
authors.affiliation: Affiliated People's Hospital of Jiangsu University Zhenjiang, Jiangsu, China.
authors.forename: Jing-Dong
authors.initials: JD
authors.lastname: Zhou
authors.affiliation: Affiliated People's Hospital of Jiangsu University Zhenjiang, Jiangsu, China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Affiliated People's Hospital of Jiangsu University Zhenjiang, Jiangsu, China.
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Zhu
mesh_terms: D000328:Adult; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics*; D005260:Female; D005557:Formaldehyde; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis*; D008875:Middle Aged; D016612:Paraffin Embedding; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D016707:Tissue Fixation; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C543845:MIRN24 microRNA, human; C526012:MIRN378 microRNA, human; D035683:MicroRNAs; D005557:Formaldehyde
keywords: Biomarkers; breast cancer; formalin-fixed paraffin-embedded; miR-24; miR-378