title: Enhanced multiparametric hyaluronan degradation for production of molar-mass-defined fragments.
abstract: Hyaluronic acid (HA) is known to serve as a dynamic mediator intervening in many physiological functions. Its specific effect has been repeatedly confirmed to be strongly influenced by the molecular size of hyaluronan fragments. However common technological approaches of HA fragments production have their limitations. In many cases, the final products do not meet the strict pharmaceutical requirements, specifically due to size polydispersity and reaction contaminants. We present novel methodology based on combination of unique incidental ability of the plant-derived protease papain to split the glycosidic bonds and an indispensable advantages of biocompatible macroporous material with incorporated ferrous ions serving as carrier for covalent papain fixation. This atypical and yet unpublished highly efficient multiparametric approach allows enhanced HA fragmentation for easily and safely producing molar-mass-defined HA fragments with narrow size distribution. Native polyacrylamide gel electrophoresis (PAGE) and size exclusion chromatography/multi-angle light scattering (SEC-MALS) confirmed the effectiveness of our multiparametric approach.
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 3210 Pardubice, Czech Republic; Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 3210 Pardubice, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Holubova
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 3210 Pardubice, Czech Republic. Electronic address: lucie.korecka@upce.cz.
authors.forename: Lucie
authors.initials: L
authors.lastname: Korecka
authors.affiliation: Synthetic Polymers, Fibres and Textiles Chemistry Unit, Institute of Chemistry and Technology of Macromolecular Materials, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 3210 Pardubice, Czech Republic.
authors.forename: Stepan
authors.initials: S
authors.lastname: Podzimek
authors.affiliation: Contipro Pharma a.s., Dolni Dobrouc 401, 561 02 Dolni Dobrouc, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Moravcova
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 3210 Pardubice, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Rotkova
authors.affiliation: Contipro Pharma a.s., Dolni Dobrouc 401, 561 02 Dolni Dobrouc, Czech Republic.
authors.forename: Tereza
authors.initials: T
authors.lastname: Ehlova
authors.affiliation: Contipro Pharma a.s., Dolni Dobrouc 401, 561 02 Dolni Dobrouc, Czech Republic.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Velebny
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 3210 Pardubice, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Bilkova
mesh_terms: D002482:Cellulose / Q000737:chemistry; D002850:Chromatography, Gel; D004800:Enzymes, Immobilized / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry*; D006821:Hyaluronoglucosaminidase / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D060328:Magnetic Phenomena; D008970:Molecular Weight; D063347:Native Polyacrylamide Gel Electrophoresis; D010206:Papain / Q000737:chemistry; D012542:Scattering, Radiation; D014783:Viscosity
chemical_list: D004800:Enzymes, Immobilized; D002482:Cellulose; D006820:Hyaluronic Acid; D007501:Iron; D006821:Hyaluronoglucosaminidase; D010206:Papain
keywords: Hyaluronan fragmentation; Magnetic particles; Papain; Pharmaceutics; SEC-MALS