title: Manganese nanoparticles: impact on non-nodulated plant as a potent enhancer in nitrogen metabolism and toxicity study both in vivo and in vitro.
abstract: Mung bean plants were grown under controlled conditions and supplemented with macro- and micronutrients. The objective of this study was to determine the response of manganese nanoparticles (MnNP) in nitrate uptake, assimilation, and metabolism compared with the commercially used manganese salt, manganese sulfate (MS). MnNP was modulated to affect the assimilatory process by enhancing the net flux of nitrogen assimilation through NR-NiR and GS-GOGAT pathways. This study was associated with toxicological investigation on in vitro and in vivo systems to promote MnNP as nanofertilizer and can be used as an alternative to MS. MnNP did not impart any toxicity to the mice brain mitochondria except in the partial inhibition of complex II-III activity in ETC. Therefore, mitochondrial dysfunction and neurotoxicity, which were noted by excess usage of elemental manganese, were prevented. This is the first attempt to highlight the nitrogen uptake, assimilation, and metabolism in a plant system using a nanoparticle to promote a biosafe nanomicronutrient-based crop management.
authors.affiliation: Biological Sciences Division, Indian Statistical Institute , 203 B.T. Road, Kolkata 700108, India.
authors.forename: Saheli
authors.initials: S
authors.lastname: Pradhan
authors.forename: Prasun
authors.initials: P
authors.lastname: Patra
authors.forename: Shouvik
authors.initials: S
authors.lastname: Mitra
authors.forename: Kushal Kumar
authors.initials: KK
authors.lastname: Dey
authors.forename: Sneha
authors.initials: S
authors.lastname: Jain
authors.forename: Samapd
authors.initials: S
authors.lastname: Sarkar
authors.forename: Shuvrodeb
authors.initials: S
authors.lastname: Roy
authors.forename: Pratip
authors.initials: P
authors.lastname: Palit
authors.forename: Arunava
authors.initials: A
authors.lastname: Goswami
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D007887:Fabaceae / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D005308:Fertilizers / Q000032:analysis; D008297:Male; D008345:Manganese / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D050901:Nitrate Reductase / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism
chemical_list: D005308:Fertilizers; D010940:Plant Proteins; D008345:Manganese; D050901:Nitrate Reductase; D009584:Nitrogen