title: In vitro culture and characterization of human lung cancer circulating tumor cells isolated by size exclusion from an orthotopic nude-mouse model expressing fluorescent protein.
abstract: In the present study, we demonstrate an animal model and recently introduced size-based exclusion method for circulating tumor cells (CTCs) isolation. The methodology enables subsequent in vitro CTC-culture and characterization. Human lung cancer cell line H460, expressing red fluorescent protein (H460-RFP), was orthotopically implanted in nude mice. CTCs were isolated by a size-based filtration method and successfully cultured in vitro on the separating membrane (MetaCell®), analyzed by means of time-lapse imaging. The cultured CTCs were heterogeneous in size and morphology even though they originated from a single tumor. The outer CTC-membranes were blebbing in general. Abnormal mitosis resulting in three daughter cells was frequently observed. The expression of RFP ensured that the CTCs originated from lung tumor. These readily isolatable, identifiable and cultivable CTCs can be used to characterize individual patient cancers and for screening of more effective treatment.
authors.affiliation: Department of Laboratory Genetics, University Hospital Kralovske Vinohrady, Srobarova 50, 100 34, Prague, Czech Republic.
authors.forename: Katarina
authors.initials: K
authors.lastname: Kolostova
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
authors.forename: Vladimir
authors.initials: V
authors.lastname: Bobek
mesh_terms: D000818:Animals; D002469:Cell Separation; D004195:Disease Models, Animal; D006801:Humans; D008164:Luminescent Proteins / Q000096:biosynthesis*; D008175:Lung Neoplasms / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation / Q000473:pathology; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D010316:Particle Size; D016062:Porosity; D013499:Surface Properties; D014407:Tumor Cells, Cultured; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein