title: Analysis of methylation and mRNA expression status of FADD and FAS genes in patients with oral squamous cell carcinoma.
abstract: BACKGROUND
  Apoptosis is an important mechanism that is responsible for the physiological deletion of harmful, damaged, or unwanted cells. Changed expression of apoptosis-related genes may lead to abnormal cell proliferation and finally to tumor genesis. Our aims were to analyze the promoter methylation and gene expression profiles of FADD and FAS genes in risk of OSCC.
  
  
  METHODS
  we analyze the promoter methylation status of FADD and FAS genes using Methylation - Specific PCR (MSP) in 86 OSCC tissues were kept in paraffin and 68 normal oral tissues applied as control. Also, FADD and FAS genes expression were analyzed in 19 cases and 20 normal specimens by Real-Time Reverse-Transcripts PCR.
  
  
  RESULTS
  Aberrant promoter methylation of FADD and FAS genes were detected in 12.79 % (11 of 86) and 60.46 % (52 of 86) of the OSCC cases, respectively, with a significant difference between cases and healthy controls for both FADD and FAS genes (P < 0.001). The gene expression analysis showed statistically significant difference between cases and healthy controls for both FADD (p<0.02) and FAS (p<0.007) genes.
  
  
  CONCLUSIONS
  To the best our knowledge, the data of this study are the first report regarding, the effect of promoter hypermethylation of the FADD and FAS genes in development of OSCC. To confirm the data, it is recommended doing further study in large sample sizes in various genetic populations.
authors.affiliation: Department of Biology, University of Sistan and Baluchestan, Zahedan, PO Box 98155-987, Iran, dor_kordi@yahoo.com.
authors.forename: Eshaghali
authors.initials: E
authors.lastname: Saberi
authors.forename: Dor-Mohammad
authors.initials: DM
authors.lastname: Kordi-Tamandani
authors.forename: Sara
authors.initials: S
authors.lastname: Jamali
authors.forename: Mohammad-Ayoub
authors.initials: MA
authors.lastname: Rigi-Ladiz
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism*; D019175:DNA Methylation; D053200:Fas-Associated Death Domain Protein / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D019014:fas Receptor / Q000235:genetics* / Q000378:metabolism*
chemical_list: C505533:FADD protein, human; C491901:FAS protein, human; D053200:Fas-Associated Death Domain Protein; D012333:RNA, Messenger; D019014:fas Receptor