title: Profiling of serum metabolites in canine lymphoma using gas chromatography mass spectrometry.
abstract: Canine lymphoma is a common cancer that has high rates of complete remission with combination chemotherapy. However, the duration of remission varies based on multiple factors, and there is a need to develop a method for early detection of recurrence. In this study, we compared the metabolites profiles in serum from 21 dogs with lymphoma and 13 healthy dogs using gas chromatography mass spectrometry (GC-MS). The lymphoma group was separated from the control group in an orthogonal projection to latent structure with discriminant analysis (OPLS-DA) plot using ions of m/z 100-600, indicating that the metabolites profiles in lymphoma cases differed from those in healthy dogs. The lymphoma group was also separated from the control group on OPLS-DA plot using 29 metabolites identified in all serum samples. Significant differences were found for 16 of these metabolites with higher levels in the lymphoma group for 15 of the metabolites and lower levels for inositol. An OPLS-DA plot showed separation of the lymphoma and healthy groups using these 16 metabolites only. These results indicate that metabolites profile with GC-MS may be a useful tool for detection of potential biomarker and diagnosis of canine lymphoma.
authors.affiliation: Laboratory of Cellular and Molecular Biology, Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, Japan.
authors.forename: Reo
authors.initials: R
authors.lastname: Tamai
authors.forename: Masaru
authors.initials: M
authors.lastname: Furuya
authors.forename: Shingo
authors.initials: S
authors.lastname: Hatoya
authors.forename: Hideo
authors.initials: H
authors.lastname: Akiyoshi
authors.forename: Ryohei
authors.initials: R
authors.lastname: Yamamoto
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Komori
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Yokoi
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Tani
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hirano
authors.forename: Masayuki
authors.initials: M
authors.lastname: Komori
authors.forename: Shigeo
authors.initials: S
authors.lastname: Takenaka
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood*; D016002:Discriminant Analysis; D004283:Dog Diseases / Q000097:blood* / Q000175:diagnosis*; D004285:Dogs; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods / Q000662:veterinary*; D008223:Lymphoma / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D008297:Male; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis / Q000662:veterinary*; D018709:Statistics, Nonparametric
chemical_list: D014408:Biomarkers, Tumor