title: Putative PmrA and PmcA are important for normal growth, morphogenesis and cell wall integrity, but not for viability in Aspergillus nidulans.
abstract: P-type Ca(2+)-transporting ATPases are Ca(2+) pumps, extruding cytosolic Ca(2+) to the extracellular environment or the intracellular Ca(2+) store lumens. In budding yeast, Pmr1 (plasma membrane ATPase related), and Pmc1 (plasma membrane calcium-ATPase) cannot be deleted simultaneously for it to survive in standard medium. Here, we deleted two putative Ca(2+) pumps, designated AnPmrA and AnPmcA, from Aspergillus nidulans, and obtained the mutants ΔanpmrA and ΔanpmcA, respectively. Then, using ΔanpmrA as the starting strain, the promoter of its anpmcA was replaced with the alcA promoter to secure the mutant ΔanpmrAalcApmcA or its anpmcA was deleted completely to produce the mutant ΔanpmrAΔpmcA. Different from the case in Saccharomyces cerevisiae, double deletion of anpmrA and anpmcA was not lethal in A. nidulans. In addition, deletion of anpmrA and/or anpmcA had produced growth defects, although overexpression of AnPmc1 in ΔanpmrAalcApmcA could not restore the growth defects that resulted from the loss of AnPmrA. Moreover, we found AnPmrA was indispensable for maintenance of normal morphogenesis, especially in low-Ca(2+)/Mn(2+) environments. Thus, our findings suggest AnPmrA and AnPmcA might play important roles in growth, morphogenesis and cell wall integrity in A. nidulans in a different way from that in yeasts.
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology; College of Life Sciences, Nanjing Normal University, Nanjing 210023, PR China.
authors.forename: Hechun
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology; College of Life Sciences, Nanjing Normal University, Nanjing 210023, PR China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology; College of Life Sciences, Nanjing Normal University, Nanjing 210023, PR China.
authors.forename: Shizhu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology; College of Life Sciences, Nanjing Normal University, Nanjing 210023, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Lu
mesh_terms: D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002473:Cell Wall / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D050296:Microbial Viability; D053499:Plasma Membrane Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D017384:Sequence Deletion
chemical_list: D005656:Fungal Proteins; C433210:pmrA protein, Aspergillus niger; D053499:Plasma Membrane Calcium-Transporting ATPases; D002118:Calcium