title: Bacterial colonization of the ovarian bursa in dogs with clinically suspected pyometra and in controls.
abstract: Septic peritonitis occurs relatively commonly in dogs. Secondary septic peritonitis is usually associated with perforation of intestines or infected viscera, such as the uterus in pyometra cases. The aim of this study was to evaluate the bacterial flora in the ovarian bursae of intact bitches as a potential source of contamination. One hundred forty dogs, clinically suspected of pyometra, were prospectively enrolled. The control group consisted of 26 dogs that underwent elective ovariohysterectomies and 18 dogs with mammary gland tumors that were neutered at the time of mastectomy. Bacteriology samples were taken aseptically at the time of surgery from the bursae and the uterus in all dogs. Twenty-two dogs that were clinically suspected of pyometra had sterile uterine content ("mucometra" cases); the remaining 118 had positive uterine cultures ("pyometra" cases) and septic peritoneal fluid was present in 10% of these cases. Of the 118 pyometra cases, 9 had unilateral and 15 had bilateral bacterial colonization of their ovarian bursae. However, the bacteria from the ovarian bursa were similar to those recovered from the uterine pus in only half of the cases. Furthermore, positive bursae were also seen in one mucometra dog (unilateral) and in four control dogs (two unilateral and two bilateral). The data illustrate that the canine ovarian bursa can harbor bacteria. The biological importance of these isolations remains unclear.
authors.affiliation: Department of Small Animal Medicine and Clinical Biology, Faculty of Veterinary Medicine, Ghent University, Belgium.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Rubio
authors.affiliation: Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Belgium.
authors.forename: Filip
authors.initials: F
authors.lastname: Boyen
authors.affiliation: Department of Small Animal Medicine and Clinical Biology, Faculty of Veterinary Medicine, Ghent University, Belgium.
authors.forename: Olaf
authors.initials: O
authors.lastname: Tas
authors.affiliation: Department of Small Animal Medicine and Clinical Biology, Faculty of Veterinary Medicine, Ghent University, Belgium.
authors.forename: Adriaan
authors.initials: A
authors.lastname: Kitshoff
authors.affiliation: Department of Small Animal Medicine and Clinical Biology, Faculty of Veterinary Medicine, Ghent University, Belgium.
authors.forename: Ingeborgh
authors.initials: I
authors.lastname: Polis
authors.affiliation: Department of Small Animal Medicine and Clinical Biology, Faculty of Veterinary Medicine, Ghent University, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: Van Goethem
authors.affiliation: Department of Small Animal Medicine and Clinical Biology, Faculty of Veterinary Medicine, Ghent University, Belgium. Electronic address: hilde.derooster@ugent.be.
authors.forename: Hilde
authors.initials: H
authors.lastname: de Rooster
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000382:microbiology / Q000473:pathology*; D004285:Dogs; D005260:Female; D007044:Hysterectomy / Q000662:veterinary; D010052:Ovariectomy / Q000662:veterinary; D010053:Ovary / Q000382:microbiology*; D010538:Peritonitis / Q000473:pathology / Q000662:veterinary; D055112:Pyometra / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D014599:Uterus / Q000473:pathology
keywords: Bacteria; Dog; Ovarian bursa; Peritonitis; Pyometra; Uterus