title: Alleviation of respiratory syncytial virus replication and inflammation by fungal immunomodulatory protein FIP-fve from Flammulina velutipes.
abstract: Respiratory syncytial virus (RSV) causes bronchiolitis in children followed by inflammation and asthma-like symptoms. The development of preventive therapy for this virus continues to pose a challenge. Fungal immunomodulatory proteins (FIPs) exhibit anti-inflammatory function. FIP-fve is an immunomodulatory protein isolated from Flammulina velutipes. To determine whether FIP-fve affects the infection or consequence of immunity of RSV, we investigated viral titers of RSV and inflammatory cytokine levels in vivo and in vitro. Oral FIP-fve decreased RSV-induced airway hyperresponsiveness (AHR), airway inflammation, and IL-6 expression in bronchoalveolar lavage fluid (BALF) of BALB/c mice. RSV replication and interleukin 6 (IL-6) levels in RSV-infected HEp-2 cells were compared before and after FIP-fve treatment. FIP-fve inhibited viral titers on plaque assay and Western blot, as well as inhibited RSV-stimulated expression of IL-6 on ELISA and RT-PCR. The results of this study suggested that FIP-fve decreases RSV replication, RSV-induced inflammation and respiratory pathogenesis. FIP-fve is a widely used, natural compound from F.velutipes that may be a safe agent for viral prevention and even therapy.
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
authors.forename: Yu-Chi
authors.initials: YC
authors.lastname: Chang
authors.affiliation: Vaccine Research and Development Center, Miaoli County, National Health Research Institutes, Miaoli, Taiwan.
authors.forename: Yen-Hung
authors.initials: YH
authors.lastname: Chow
authors.affiliation: Division of Allergy, Department of Pediatrics, Chung Shan Medical University Hospital, Taichung, Taiwan.
authors.forename: Hai-Lun
authors.initials: HL
authors.lastname: Sun
authors.affiliation: Department of Biomedical Sciences, Chung Shan Medical University, Taichung, Taiwan.
authors.forename: Yu-Fan
authors.initials: YF
authors.lastname: Liu
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
authors.forename: Yu-Tzu
authors.initials: YT
authors.lastname: Lee
authors.affiliation: Division of Allergy, Department of Pediatrics, Chung Shan Medical University Hospital, Taichung, Taiwan; School of Medicine, Chung Shan Medical University, Taichung, Taiwan. Electronic address: cshy095@csh.org.tw.
authors.forename: Ko-Huang
authors.initials: KH
authors.lastname: Lue
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Division of Allergy, Department of Pediatrics, Chung Shan Medical University Hospital, Taichung, Taiwan. Electronic address: jlko@csmu.edu.tw.
authors.forename: Jiunn-Liang
authors.initials: JL
authors.lastname: Ko
mesh_terms: D000818:Animals; D001990:Bronchiolitis, Viral / Q000276:immunology / Q000517:prevention & control*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D002460:Cell Line; D005260:Female; D005656:Fungal Proteins / Q000276:immunology / Q000494:pharmacology*; D006801:Humans; D056747:Immunomodulation / Q000187:drug effects; D007249:Inflammation / Q000188:drug therapy* / Q000276:immunology; D007372:Interferons / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D012130:Respiratory Hypersensitivity / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D018357:Respiratory Syncytial Virus Infections / Q000188:drug therapy* / Q000276:immunology; D012136:Respiratory Syncytial Viruses / Q000254:growth & development / Q000276:immunology*; D014779:Virus Replication / Q000187:drug effects*
chemical_list: C547132:FIP-fve protein, Flammulina velutipes; D005656:Fungal Proteins; D015850:Interleukin-6; D016328:NF-kappa B; D007372:Interferons
keywords: Antiviral agent; FIP-fve; Immunomodulatory protein; Inflammatory; NF-κB; Respiratory syncytial virus