title: Analysis of Rab GTPase-effector interactions by bimolecular fluorescence complementation.
abstract: RAB GTPases interact with specific effector molecules in a spatiotemporally regulated manner to induce various downstream reactions. To clarify the overall picture of RAB GTPase functions, it is important to elucidate the cellular locale where RAB and its effectors interact. Here, we applied a bimolecular fluorescence complementation (BiFC) assay to analyze where RAB GTPase interacted with effectors in endosomal trafficking.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.
authors.forename: Emi
authors.initials: E
authors.lastname: Ito
authors.forename: Takashi
authors.initials: T
authors.lastname: Ueda
mesh_terms: D011992:Endosomes / Q000378:metabolism / Q000648:ultrastructure*; D018613:Microscopy, Confocal; D008967:Molecular Biology / Q000379:methods*; D021381:Protein Transport / Q000235:genetics; D020691:rab GTP-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D020691:rab GTP-Binding Proteins