title: Integrative chemical proteomics and cell biology methods to study endocytosis and vesicular trafficking in Arabidopsis.
abstract: We present a comprehensive approach combining proteomics and cell biology to study vesicular trafficking in plants. Within this approach, we exploit chemical compounds inhibiting particular vesicular trafficking events in plant cells. Treatment of plants with these relatively specific inhibitors results in intracellular accumulation of proteins being transported by vesicles as well as in a change in abundance of regulatory proteins. Such pharmacological inhibition allows for identification of key proteins, and for further detailed functional investigation using cell biological, molecular biological, and biochemical methods used for validation of proteomic results.
authors.affiliation: Department of Cell Biology, Faculty of Science, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University Olomouc, Šlechtitelů 11, 783 71, Olomouc, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Takáč
authors.forename: Tibor
authors.initials: T
authors.lastname: Pechan
authors.forename: Olga
authors.initials: O
authors.lastname: Samajová
authors.forename: Jozef
authors.initials: J
authors.lastname: Samaj
mesh_terms: D000730:Androstadienes / Q000008:administration & dosage; D017360:Arabidopsis / Q000187:drug effects; D029681:Arabidopsis Proteins / Q000096:biosynthesis; D020126:Brefeldin A / Q000008:administration & dosage; D004705:Endocytosis / Q000187:drug effects*; D008967:Molecular Biology / Q000379:methods*; D040901:Proteomics; D022161:Transport Vesicles / Q000187:drug effects*; D033921:Vesicular Transport Proteins / Q000096:biosynthesis; D000077191:Wortmannin
chemical_list: D000730:Androstadienes; D029681:Arabidopsis Proteins; D033921:Vesicular Transport Proteins; D020126:Brefeldin A; D000077191:Wortmannin