title: Identification of crypto- and neochlorogenic lactones as potent xanthine oxidase inhibitors in roasted coffee beans.
abstract: Xanthine oxidase (XO) inhibitory activity has been found in boiling water extracts from roasted coffee beans. Therefore, assay-guided purification of the extracts was performed using size-exclusion column chromatography, and subsequently with reversed phase HPLC to afford lactone derivatives of chlorogenic acids. Among the tested lactones, crypto- and neochlorogenic lactones showed potent XO inhibitory activities compared with three major chlorogenic acids found in coffee beans. These XO inhibitory lactones may ameliorate gout and hyperuricemia in humans who drink coffee.
authors.affiliation: a Graduate School of Integrated Arts and Sciences , University of Tokushima , Tokushima , Japan.
authors.forename: Sari
authors.initials: S
authors.lastname: Honda
authors.forename: Yukari
authors.initials: Y
authors.lastname: Miura
authors.forename: Akiko
authors.initials: A
authors.lastname: Masuda
authors.forename: Toshiya
authors.initials: T
authors.lastname: Masuda
mesh_terms: D002726:Chlorogenic Acid / Q000737:chemistry / Q000302:isolation & purification*; D040503:Coffea / Q000737:chemistry*; D003296:Cooking; D006358:Hot Temperature; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry; D012639:Seeds / Q000737:chemistry*; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors* / Q000737:chemistry
chemical_list: D007783:Lactones; D010936:Plant Extracts; D002726:Chlorogenic Acid; D014969:Xanthine Oxidase
keywords: chlorogenic acid; cryptochlorogenic lactone; neochlorogenic lactone; roasted coffee beans; xanthine oxidase inhibition