title: Evaluation of epithelial chimerism after bone marrow mesenchymal stromal cell infusion in intestinal transplant patients.
abstract: Intestinal transplantation is the most effective treatment for patients with short bowel syndrome and small bowel insufficiencies. We evaluated epithelial chimerism after infusion of autologous bone marrow mesenchymal stromal cells (BMSCs) in patients undergoing cadaveric donor isolated intestinal transplantation (I-ITx). BMSCs were isolated from patients' bone marrow via iliac puncture and expanded in vitro prior to infusion. Two out of the 3 patients were infused with autologous BMSCs, and small intestine tissue biopsies collected post-operatively were analyzed for epithelial chimerism using XY fluorescent in situ hybridization and short tandem repeat polymerase chain reaction. We observed epithelial chimeric effect in conditions both with and without BMSC infusion. Although our results suggest a higher epithelial chimerism effect with autologous BMSC infusion in I-ITx, the measurements in multiple biopsies at different time points that demonstrate the reproducibility of this finding and its stability or changes in the level over time would be beneficial. These approaches may have potential implications for improved graft survival, lower immunosuppressant doses, superior engraftment of the transplanted tissue, and higher success rates in I-ITx.
authors.affiliation: Organ Transplantation Center, Department of General Surgery, Tepecik Training and Research Hospital, Izmir, Turkey. Electronic address: selcukkilinc79@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Kilinc
authors.affiliation: Case Biomanufacturing and Microfabrication Laboratory, Mechanical and Aerospace Engineering, Case Western Reserve University, Cleveland, Ohio, USA.
authors.forename: U A
authors.initials: UA
authors.lastname: Gurkan
authors.affiliation: Bio-acoustic MEMS in Medicine Laboratory, Division of Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts, USA.
authors.forename: S
authors.initials: S
authors.lastname: Guven
authors.affiliation: Institute of Forensic Medicine, Izmir, Turkey.
authors.forename: G
authors.initials: G
authors.lastname: Koyuncu
authors.affiliation: Department of General Surgery, Dr Lütfi Kırdar Kartal Training and Research Hospital, Istanbul, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Tan
authors.affiliation: Organ Transplantation Center, Department of General Surgery, Tepecik Training and Research Hospital, Izmir, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Karaca
authors.affiliation: Department of Cardiology, Tepecik Training and Research Hospital, Izmir, Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Ozdogan
authors.affiliation: Organ Transplantation Center, Department of General Surgery, Tepecik Training and Research Hospital, Izmir, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Dogan
authors.affiliation: Organ Transplantation Center, Department of General Surgery, Tepecik Training and Research Hospital, Izmir, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Tugmen
authors.affiliation: Department of Pathology, Tepecik Training and Research Hospital, Izmir, Turkey.
authors.forename: E E
authors.initials: EE
authors.lastname: Pala
authors.affiliation: Department of Pathology, Tepecik Training and Research Hospital, Izmir, Turkey.
authors.forename: U
authors.initials: U
authors.lastname: Bayol
authors.affiliation: Department of Pediatrics, Tepecik Training and Research Hospital, Izmir, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Baran
authors.affiliation: Department of Biochemistry, Tepecik Training and Research Hospital, Izmir, Turkey.
authors.forename: Y
authors.initials: Y
authors.lastname: Kurtulmus
authors.affiliation: Department of Biochemistry, Tepecik Training and Research Hospital, Izmir, Turkey.
authors.forename: I
authors.initials: I
authors.lastname: Pirim
authors.affiliation: Organ Transplantation Center, Department of General Surgery, Tepecik Training and Research Hospital, Izmir, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Kebapci
authors.affiliation: Bio-acoustic MEMS in Medicine Laboratory, Division of Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts, USA; Harvard-Massachusetts Institute of Technology (MIT) Health Sciences and Technology, Cambridge, Massachusetts, USA. Electronic address: udemirci@rics.bwh.harvard.edu.
authors.forename: U
authors.initials: U
authors.lastname: Demirci
mesh_terms: D000293:Adolescent; D000328:Adult; D016026:Bone Marrow Transplantation / Q000379:methods*; D002648:Child; D046528:Chimerism; D005260:Female; D006085:Graft Survival; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007421:Intestine, Small / Q000637:transplantation*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D015203:Reproducibility of Results; D014019:Tissue Donors; D055815:Young Adult
chemical_list: D007166:Immunosuppressive Agents