title: An alteration in ATG16L1 stability in Crohn disease.
abstract: Individuals who harbor a common coding polymorphism (Thr300Ala) within a structurally unclassified region of ATG16L1 are at increased risk for the development of Crohn disease. Recently, we reported on the generation and characterization of knockin mice carrying the ATG16L1 T300A variant. We demonstrate that multiple cell types from T300A knock-in mice exhibit reduced selective autophagy, and we mechanistically link this phenotype with an increased susceptibility of ATG16L1 T300A to CASP3- and CASP7-mediated cleavage. These findings demonstrate how a single polymorphism can result in cell type- and pathway-specific disruptions of selective autophagy and alterations in the inflammatory milieu that can contribute to disease.
authors.affiliation: Broad Institute; Cambridge, MA USA; Center for Computational and Integrative Biology; Massachusetts General Hospital; Boston, MA USA.
authors.forename: Kara G
authors.initials: KG
authors.lastname: Lassen
authors.affiliation: Broad Institute; Cambridge, MA USA; Center for Computational and Integrative Biology; Massachusetts General Hospital; Boston, MA USA; Gastrointestinal Unit and Center for the Study of Inflammatory Bowel Disease; Massachusetts General Hospital; Harvard Medical School; Boston, MA USA.
authors.forename: Ramnik J
authors.initials: RJ
authors.lastname: Xavier
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D003424:Crohn Disease / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D051379:Mice; D008954:Models, Biological; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D055550:Protein Stability; D015398:Signal Transduction
chemical_list: D002352:Carrier Proteins
keywords: ATG16L1; Crohn disease; IL1B; antibacterial autophagy; caspase cleavage