title: From pathways to networks: connecting dots by establishing protein-protein interaction networks in signaling pathways using affinity purification and mass spectrometry.
abstract: Signal transductions are the basis of biological activities in all living organisms. Studying the signaling pathways, especially under physiological conditions, has become one of the most important facets of modern biological research. During the last decade, MS has been used extensively in biological research and is proven to be effective in addressing important biological questions. Here, we review the current progress in the understanding of signaling networks using MS approaches. We will focus on studies of protein-protein interactions that use affinity purification followed by MS approach. We discuss obstacles to affinity purification, data processing, functional validation, and identification of transient interactions and provide potential solutions for pathway-specific proteomics analysis, which we hope one day will lead to a comprehensive understanding of signaling networks in humans.
authors.affiliation: Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.forename: Wenqi
authors.initials: W
authors.lastname: Wang
authors.forename: Junjie
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D025941:Protein Interaction Mapping / Q000379:methods*; D060066:Protein Interaction Maps; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction
chemical_list: D011506:Proteins; D017346:Protein Serine-Threonine Kinases
keywords: Affinity purification; Animal proteomics; Hippo signaling; Mass spectrometry; Protein-protein interaction; Signaling pathways