title: In vitro anticancer activity of loquat tea by inducing apoptosis in human leukemia cells.
abstract: Fresh loquat leaves have been used as folk health herb in Asian countries for long time, although the evidence supporting their functions is still minimal. This study aimed to clarify the chemopreventive effect of loquat tea extract (LTE) by investigating the inhibition on proliferation, and underlying mechanisms in human promyelocytic leukemia cells (HL-60). LTE inhibited proliferation of HL-60 in a dose-dependent manner. Molecular data showed that the isolated fraction of LTE induced apoptosis of HL-60 as characterized by DNA fragmentation; activation of caspase-3, -8, and -9; and inactivation of poly(ADP)ribose polymerase. Moreover, LTE fraction increased the ratio of pro-apoptotic Bcl-2-associated X protein (Bax)/anti-apoptotic myeloid cell leukemia 1 (Mcl-1) that caused mitochondrial membrane potential loss and cytochrome c released to cytosol. Thus, our data indicate that LTE might induce apoptosis in HL-60 cells through a mitochondrial dysfunction pathway. These findings enhance our understanding for chemopreventive function of loquat tea.
authors.affiliation: a United Graduate School of Agricultural Sciences, Kagoshima University , Kagoshima , Japan.
authors.forename: Phyu Phyu Khine
authors.initials: PP
authors.lastname: Zar
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yano
authors.forename: Kozue
authors.initials: K
authors.lastname: Sakao
authors.forename: Fumio
authors.initials: F
authors.lastname: Hashimoto
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nakano
authors.forename: Makoto
authors.initials: M
authors.lastname: Fujii
authors.forename: De-Xing
authors.initials: DX
authors.lastname: Hou
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001628:Beverages / Q000032:analysis*; D001713:Biphenyl Compounds / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D031983:Eriobotrya / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D064549:Myeloid Cell Leukemia Sequence 1 Protein / Q000378:metabolism; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; C579095:MCL1 protein, human; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D010851:Picrates; D010936:Plant Extracts; D051028:bcl-2-Associated X Protein; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: HL-60 cells; apoptosis; caspase; loquat tea extract; mitochondria