title: Biocompatibility and efficacy of collagen/gelatin sponge scaffold with sustained release of basic fibroblast growth factor on vocal fold fibroblasts in 3-dimensional culture.
abstract: OBJECTIVE
  Treatment of vocal fold scarring remains challenging. We have previously reported the therapeutic effects of local injection of basic fibroblast growth factor (bFGF) in animal models and humans. A novel collagen/gelatin sponge (CGS) is capable of sustained release of bFGF, which compensates for its quick absorption in vivo, avoiding multiple injections. This study aimed to evaluate the biocompatibility and efficacy of the CGS in rat vocal fold fibroblasts prior to human trials.
  
  
  METHODS
  Fibroblasts extracted from Sprague-Dawley rat vocal folds were seeded onto a CGS and then cultivated with bFGF at concentrations of 0, 10, and 100 ng/mL. Vocal fold fibroblast morphology, adhesion, proliferation, and gene expression were measured under these 3-dimensional conditions.
  
  
  RESULTS
  Cells adhered to the CGS from day 1. Although no significant differences in cell morphology were detected, cell proliferation was accelerated by bFGF administration. Expression of endogenous bFGF and hepatocyte growth factor was significantly up-regulated at 10 ng/mL bFGF. The expression of procollagen I and procollagen III was significantly suppressed, whereas HAS-1 and HAS-2 were up-regulated at 10 and 100 ng/mL bFGF.
  
  
  CONCLUSIONS
  The collagen/gelatin sponge is biocompatible with vocal fold fibroblasts and may be useful as a bFGF drug delivery system for the treatment of scarred vocal folds.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: Nao
authors.initials: N
authors.lastname: Hiwatashi
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan hirano@ent.kuhp.kyoto-u.ac.jp.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Hirano
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Mizuta
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Tateya
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan Department of Otolaryngology-Head and Neck Surgery, Kitano Hospital, Tazuke Kofukai Medical Research Institute, Osaka, Japan.
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Kanemaru
authors.affiliation: Department of Bioartificial Organs, Institute for Frontier Medical Science, Kyoto University, Kyoto, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: Juichi
authors.initials: J
authors.lastname: Ito
authors.affiliation: Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: Katsuya
authors.initials: K
authors.lastname: Kawai
authors.affiliation: Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: Shigehiko
authors.initials: S
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D002468:Cell Physiological Phenomena / Q000187:drug effects*; D002921:Cicatrix; D003094:Collagen / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D016503:Drug Delivery Systems; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage*; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D005780:Gelatin / Q000494:pharmacology*; D005781:Gelatin Sponge, Absorbable / Q000494:pharmacology*; D006133:Growth Substances / Q000008:administration & dosage; D006801:Humans; D008422:Materials Testing / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds; D014827:Vocal Cords / Q000473:pathology*; D014832:Voice Disorders / Q000188:drug therapy
chemical_list: D001672:Biocompatible Materials; D006133:Growth Substances; D016222:Fibroblast Growth Factor 2; D005780:Gelatin; D003094:Collagen
keywords: basic fibroblast growth factor; collagen scaffold; gene expression; sustained release; vocal fold