title: Validation of lignocellulosic biomass carbohydrates determination via acid hydrolysis.
abstract: This work studied the two-step acid hydrolysis for determining carbohydrates in lignocellulosic biomass. Estimation of sugar loss based on acid hydrolyzed sugar standards or analysis of sugar derivatives was investigated. Four model substrates (starch, holocellulose, filter paper and cotton) and three levels of acid/material ratios (7.8, 10.3 and 15.4, v/w) were studied to demonstrate the range of test artifacts. The method for carbohydrates estimation based on acid hydrolyzed sugar standards having the most satisfactory carbohydrate recovery and relative standard deviation. Raw material and the acid/material ratio both had significant effect on carbohydrate hydrolysis, suggesting the acid to have impacts beyond a catalyst in the hydrolysis. Following optimal procedures, we were able to reach a carbohydrate recovery of 96% with a relative standard deviation less than 3%. The carbohydrates recovery lower than 100% was likely due to the incomplete hydrolysis of substrates, which was supported by scanning electron microscope (SEM) images.
authors.affiliation: Department of Biological System Engineering, University of Wisconsin, Agricultural Engineering Building, Madison, WI 53706, USA. Electronic address: szhou34@wisc.edu.
authors.forename: Shengfei
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Biological System Engineering, University of Wisconsin, Agricultural Engineering Building, Madison, WI 53706, USA. Electronic address: trunge@wbi.wisc.edu.
authors.forename: Troy M
authors.initials: TM
authors.lastname: Runge
mesh_terms: D018533:Biomass; D002241:Carbohydrates / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008956:Models, Chemical
chemical_list: D002241:Carbohydrates; C036909:lignocellulose; D008031:Lignin
keywords: Carbohydrates SEM; Carbohydrates determination; High-performance anion-exchange chromatography; Incomplete hydrolysis; Sugar loss; Two-step acid hydrolysis