title: Spherical nucleic acids as a divergent platform for synthesizing RNA-nanoparticle conjugates through enzymatic ligation.
abstract: Herein, we describe a rapid, divergent method for using spherical nucleic acids (SNAs) as a universal platform for attaching RNA to DNA-modified nanoparticles using enzyme-mediated techniques. This approach provides a sequence-specific method for the covalent attachment of one or more in vitro transcribed RNAs to a universal SNA scaffold, regardless of RNA sequence. The RNA-nanoparticle constructs are shown to effectively knock down two different gene targets using a single, dual-ligated nanoparticle construct.
authors.affiliation: Department of Chemistry and ‡International Institute for Nanotechnology, §Interdisciplinary Biological Sciences Graduate Program, Northwestern University , 2145 Sheridan Road, Evanston, Illinois 60208-3113, United States.
authors.forename: Jessica L
authors.initials: JL
authors.lastname: Rouge
authors.forename: Liangliang
authors.initials: L
authors.lastname: Hao
authors.forename: Xiaochen A
authors.initials: XA
authors.lastname: Wu
authors.forename: William E
authors.initials: WE
authors.lastname: Briley
authors.forename: Chad A
authors.initials: CA
authors.lastname: Mirkin
mesh_terms: D001483:Base Sequence; D011088:DNA Ligases / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000737:chemistry* / Q000235:genetics
chemical_list: D012313:RNA; D011088:DNA Ligases
keywords: T4 DNA ligase; enzymatic ligation; gene knockdown; nanoparticle; siRNA