title: A Study on the Effect of Neurogenesis and Regulation of GSK3β/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats.
abstract: 170 SD rats were randomly divided to five groups. Rats in model group, no-acupuncture group, and acupuncture group were subjected to MCAO surgery. Acupuncture group received 3 consecutive acupuncture treatments at a parameter that deep in 2 mm towards apex nasi and thrust/lifted at 3 times per second for 1 minute, while model group and no-acupuncture group were no-intervention control groups. Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin(+), BrdU(+) and nestin/BrdU(+)) were found in MCAO rats which had not been observed in normal group and sham-operated group. However, the damage was attenuated by rat's "self-healing" capacity 3 days after MCAO. And the "self-healing" capacity can be strengthen by acupuncture treatment through increasing cerebral blood flow, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression. In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression in MCAO rats.
authors.affiliation: Postgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Luo
authors.affiliation: The Acupuncture & Moxibustion Institute, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China ; The Third-Level Acupuncture Dose-Effect Laboratory of the State Administration of Traditional Chinese Medicine, Tianjin 300193, China ; Tianjin Key Laboratory of Acupuncture & Moxibustion Science, Tianjin 300193, China.
authors.forename: Xiaonong
authors.initials: X
authors.lastname: Fan
authors.affiliation: Postgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Ma
authors.affiliation: Postgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Tongtao
authors.initials: T
authors.lastname: Fan
authors.affiliation: Postgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Postgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Nvzi
authors.initials: N
authors.lastname: Chang
authors.affiliation: Postgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China ; Department of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Lingxin
authors.initials: L
authors.lastname: Li
authors.affiliation: The Acupuncture & Moxibustion Institute, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China ; The Third-Level Acupuncture Dose-Effect Laboratory of the State Administration of Traditional Chinese Medicine, Tianjin 300193, China ; Tianjin Key Laboratory of Acupuncture & Moxibustion Science, Tianjin 300193, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory of Acupuncture & Moxibustion Science, Tianjin 300193, China ; Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Meng
authors.affiliation: The Acupuncture & Moxibustion Institute, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China ; Tianjin Key Laboratory of Acupuncture & Moxibustion Science, Tianjin 300193, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.affiliation: The Acupuncture & Moxibustion Institute, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China ; Tianjin Key Laboratory of Acupuncture & Moxibustion Science, Tianjin 300193, China ; Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Xuemin
authors.initials: X
authors.lastname: Shi