title: Ciona intestinalis NADH dehydrogenase NDX confers stress-resistance and extended lifespan on Drosophila.
abstract: An assembled cDNA coding for the putative single-subunit NADH dehydrogenase (NDX) of Ciona intestinalis was introduced into Drosophila melanogaster. The encoded protein was found to localize to mitochondria and to confer rotenone-insensitive substrate oxidation in organello. Transgenic flies exhibited increased resistance to menadione, starvation and temperature stress, and manifested a sex and diet-dependent increase in mean lifespan of 20-50%. However, NDX was able only weakly to complement the phenotypes produced by the knockdown of complex I subunits.
authors.affiliation: BioMediTech and Tampere University Hospital, University of Tampere, FI-33014, Finland; Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk 76025, Ukraine.
authors.forename: Dmytro V
authors.initials: DV
authors.lastname: Gospodaryov
authors.affiliation: Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk 76025, Ukraine.
authors.forename: Oleh V
authors.initials: OV
authors.lastname: Lushchak
authors.affiliation: Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk 76025, Ukraine.
authors.forename: Bohdana M
authors.initials: BM
authors.lastname: Rovenko
authors.affiliation: Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk 76025, Ukraine.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Perkhulyn
authors.affiliation: BioMediTech and Tampere University Hospital, University of Tampere, FI-33014, Finland.
authors.forename: Mike
authors.initials: M
authors.lastname: Gerards
authors.affiliation: BioMediTech and Tampere University Hospital, University of Tampere, FI-33014, Finland.
authors.forename: Tea
authors.initials: T
authors.lastname: Tuomela
authors.affiliation: BioMediTech and Tampere University Hospital, University of Tampere, FI-33014, Finland; Research Program of Molecular Neurology, University of Helsinki, FI-00014, Finland. Electronic address: howard.t.jacobs@uta.fi.
authors.forename: Howard T
authors.initials: HT
authors.lastname: Jacobs
keywords: Ageing; Alternative respiratory chain enzymes; Gene therapy; Oxidative stress; Tunicate