title: The geographic origin of Helicobacter pylori isolated from Costa Rican patients.
abstract: Helicobacter pylori infects a significant proportion of the world population and it is associated with pathologies which include chronic atrophic gastritis, peptic ulcer, and gastric neoplasias such as gastric adenocarcinoma and MALT lymphoma. Costa Rica has a high prevalence of the infection and an elevated incidence of gastric cancer and its associated mortality. The global population structure for H. pylori has been established using a MLST scheme. The population structure of the strains of H. pylori circulating in Costa Rica is currently unknown. We characterized the geographical origin of 24 H. pylori isolates from Costa Rican patients. We identified 142 new alleles for the genes included in the scheme and in eight of the 24 isolates from Costa Rican patients, all seven alleles sequenced were described for the first time. Twenty-one isolates from Costa Rican patients group with hpEurope strains and the remaining three isolates grouped with hspWAfrica isolates (Bayesian posterior probability values above 0.70, P = 0.05, after 2 000 000 generations). The obtained result in the MLST analysis was not unexpected and reflects the genetic composition of the Costa Rican population.
authors.affiliation: Instituto de Investigaciones en Salud; Universidad de Costa Rica; San José; Costa Rica.
authors.forename: Silvia E
authors.initials: SE
authors.lastname: Molina-Castro
authors.affiliation: Instituto de Investigaciones en Salud; Universidad de Costa Rica; San José; Costa Rica.
authors.forename: Dayana
authors.initials: D
authors.lastname: Herrera
authors.affiliation: Instituto de Investigaciones en Salud; Universidad de Costa Rica; San José; Costa Rica.
authors.forename: Wendy
authors.initials: W
authors.lastname: Malespín-Bendaña
authors.affiliation: Instituto de Investigaciones en Salud; Universidad de Costa Rica; San José; Costa Rica.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Ramírez
authors.affiliation: Instituto de Investigaciones en Salud; Universidad de Costa Rica; San José; Costa Rica.
authors.forename: Clas
authors.initials: C
authors.lastname: Une
mesh_terms: D016000:Cluster Analysis; D003364:Costa Rica / Q000453:epidemiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D016481:Helicobacter Infections / Q000453:epidemiology* / Q000382:microbiology*; D016480:Helicobacter pylori / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing
chemical_list: D004269:DNA, Bacterial
keywords: Costa Rica; Helicobacter pylori; MLST; alleles; phylogeny