title: DNA methylation ofmicroRNA-375in impaired glucose tolerance.
abstract: In the present study, the expression levels and DNA methylation status ofmicroRNA(miRNA)-375 in patients with impaired glucose tolerance (IGT) and type 2 diabetes mellitus (T2DM) were analyzed and the role of DNA methylation ofmiRNA-375in the pathogenesis of T2DM was investigated. Compared with themiR-375levels in patients with normal glucose tolerance (NGT; n=53), the samples from patients with IGT (n=44) exhibited downregulation ofmiR-375, while those from patients with T2DM (n=54) exhibited upregulation ofmiR-375in the plasma. Additionally, the samples from patients with IGT were observed to be hypermethylated compared with those from patients with T2DM and NGT (P=0.042). Analysis of three CpG units (CpG1.2, CpG20 and CpG25.26.27) from 17 CpG sites (between -990 and -1,258 bp, relative to the transcription start site) revealed higher methylation levels in patients with IGT compared with those in patients with NGT (P<0.05). The methylation of two CpG units (CpG1.2 and CpG25.26.27) was higher in patients with IGT than in the patients with T2DM (P<0.05). Thus, the present study demonstrated that themiR-375promoter was hypermethylated and the levels ofmiR-375in the plasma were downregulated in the patients with IGT. DNA hypomethylation may have an important role in the regulation ofmiR-375expression and may contribute to the pathogenesis of T2DM.
authors.affiliation: Departments of Endocrinology and Metabolism, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang 832002, P.R. China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.affiliation: Departments of Endocrinology and Metabolism, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang 832002, P.R. China.
authors.forename: Xiangyun
authors.initials: X
authors.lastname: Chang
authors.affiliation: Departments of Endocrinology and Metabolism, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang 832002, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Departments of Endocrinology and Metabolism, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang 832002, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yin
authors.affiliation: Departments of Endocrinology and Metabolism, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang 832002, P.R. China.
authors.forename: Kan
authors.initials: K
authors.lastname: Sun
keywords: impaired glucose tolerance; methylation; miR-375; type 2 diabetes