title: The construction of hierarchical structure on Ti substrate with superior osteogenic activity and intrinsic antibacterial capability.
abstract: The deficient osseointegration and implant-associated infections are pivotal issues for the long-term clinical success of endosteal Ti implants, while development of functional surfaces that can simultaneously overcome these problems remains highly challenging. This study aimed to fabricate sophisticated Ti implant surface with both osteogenic inducing activity and inherent antibacterial ability simply via tailoring surface topographical features. Micro/submciro/nano-scale structure was constructed on Ti by three cumulative subtractive methods, including sequentially conducted sandblasting as well as primary and secondary acid etching treatment. Topographical features of this hierarchical structure can be well tuned by the time of the secondary acid treatment. Ti substrate with mere micro/submicro-scale structure (MS0-Ti) served as a control to examine the influence of hierarchical structures on surface properties and biological activities. Surface analysis indicated that all hierarchically structured surfaces possessed exactly the same surface chemistry as that of MS0-Ti, and all of them showed super-amphiphilicity, high surface free energy, and high protein adsorption capability. Biological evaluations revealed surprisingly antibacterial ability and excellent osteogenic activity for samples with optimized hierarchical structure (MS30-Ti) when compared with MS0-Ti. Consequently, for the first time, a hierarchically structured Ti surface with topography-induced inherent antibacterial capability and excellent osteogenic activity was constructed.
authors.affiliation: 1] Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, P. R. China [2] Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, P. R. China [3].
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: 1] Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, P. R. China [2].
authors.forename: Guangyu
authors.initials: G
authors.lastname: Zha
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, P. R. China.
authors.forename: Qiaojie
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, Third Military Medical University, Chongqing 400038, P. R. China.
authors.forename: Jianxiang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, P. R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, Third Military Medical University, Chongqing 400038, P. R. China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, P. R. China.
authors.forename: Shifang
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, P. R. China.
authors.forename: Weipu
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, P. R. China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents; D018929:Cell Culture Techniques; D010006:Osteoblasts / Q000378:metabolism; D019736:Prostheses and Implants / Q000009:adverse effects; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D014025:Titanium