title: Label-free in vitro visualization of particle uptake into human oral buccal epithelial cells by confocal Raman microscopy.
abstract: In this study, we present confocal Raman microscopy for chemically selective analysis of a human buccal epithelial cell layer with a focus on label-free visualization of particle uptake into the cells. We demonstrate the suitability and benefit of this analytical technique in comparison to confocal fluorescence microscopy for three dimensional imaging of in vitro cell models.
authors.affiliation: Saarland University, Department of Biopharmaceutics and Pharmaceutical Technology, Campus A4.1, 66123 Saarbruecken, Germany. m.windbergs@mx.uni-saarland.de.
authors.forename: B
authors.initials: B
authors.lastname: Kann
authors.forename: B J
authors.initials: BJ
authors.lastname: Teubl
authors.forename: E
authors.initials: E
authors.lastname: Roblegg
authors.forename: M
authors.initials: M
authors.lastname: Windbergs
mesh_terms: D066146:Cell Body / Q000737:chemistry / Q000378:metabolism; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism; D004847:Epithelial Cells / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D006801:Humans; D018613:Microscopy, Confocal; D009061:Mouth Mucosa / Q000166:cytology; D013059:Spectrum Analysis, Raman; D014025:Titanium / Q000737:chemistry
chemical_list: C009495:titanium dioxide; D014025:Titanium