title: Hemagglutination (inhibition) assay.
abstract: The hemagglutination assay is a simple and easy method to obtain semi-quantitative data on the sugar binding and specificity of a lectin. An active lectin agglutinates erythrocytes by recognizing a carbohydrate on the cell surface and forming a cross-linked network in suspension. By serially diluting the lectin in a 96-well microtiter plate and adding a constant quantity of erythrocytes, the lectin activity can be estimated.
authors.affiliation: Graduate School of Humanities and Sciences and Glycoscience Institute, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo, 112-8610, Japan.
authors.forename: Kotone
authors.initials: K
authors.lastname: Sano
authors.forename: Haruko
authors.initials: H
authors.lastname: Ogawa
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D002469:Cell Separation; D004912:Erythrocytes / Q000166:cytology / Q000276:immunology; D005976:Glutaral / Q000378:metabolism; D006385:Hemagglutination Inhibition Tests / Q000379:methods*; D009439:Neuraminidase / Q000378:metabolism; D037121:Plant Lectins / Q000378:metabolism*; D011817:Rabbits; D013379:Substrate Specificity; D014357:Trypsin / Q000378:metabolism
chemical_list: D037121:Plant Lectins; D009439:Neuraminidase; D014357:Trypsin; D005976:Glutaral