title: Effects of different levels of dietary selenium on the proliferation of spermatogonial stem cells and antioxidant status in testis of roosters.
abstract: The objective of this study was to investigate the different levels of dietary Se (from sodium selenite) on the proliferation of SSCs (spermatogonial stem cells) in testis of roosters. Also, the antioxidant status and Se content in blood plasma and testis were evaluated. A total of eighty 12-week-old Hy-Line Variety white roosters at an averaged body weight of 1.38 ± 0.2 kg were selected and randomly divided into four experimental groups. They were fed with the basal diet (0.044 mgSe/kg DM) supplemented with 0 (control), 0.5, 1.0 or 2.0 mgSe/kg DM (from sodium selenite). After the feeding experiment, blood and testis samples were collected for analysis of the antioxidant status and Se concentration. The testis samples were also used to examine the Thy-1 and β1-integrin mRNA expression by RT-PCR and detect the population of SSCs by immunofluorescence analysis. The results show that Se concentration in blood and testis of the animals was progressively increased with the increasing Se level in diet. The highest GSH-Px (glutathione peroxidase) activity and lowest MDA content in blood and testis was obtained in the treatment of 0.5mg/kg. RT-PCR analysis showed that mRNA expression of SSCs markers were significantly lower in the control and 1.0mg/kg groups when compared with that in the treatment of 0.5mg/kg. A similar trend was observed in the population of SSCs analyzed by immunofluorescence assay. These data suggest that dietary Se can influence the population of SSCs of roosters during spermatogenesis and that oxidative stress can modulate SSCs behavior through regulating some key factors during spermatogenesis.
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, PR China; Lab of Animal Reproductive biotechnology, Shanxi Agricultural University, Taigu 030801, PR China. Electronic address: sxausl@gmail.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, PR China; Lab of Animal Reproductive biotechnology, Shanxi Agricultural University, Taigu 030801, PR China.
authors.forename: Youshe
authors.initials: Y
authors.lastname: Ren
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, PR China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Yao
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, PR China.
authors.forename: Ruigao
authors.initials: R
authors.lastname: Song
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, PR China; Lab of Animal Reproductive biotechnology, Shanxi Agricultural University, Taigu 030801, PR China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Yue
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D049109:Cell Proliferation; D002645:Chickens / Q000502:physiology*; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D008297:Male; D018038:Sodium Selenite / Q000008:administration & dosage / Q000494:pharmacology*; D013093:Spermatogonia / Q000166:cytology* / Q000502:physiology; D013234:Stem Cells / Q000187:drug effects* / Q000502:physiology; D013737:Testis / Q000187:drug effects* / Q000502:physiology
chemical_list: D000975:Antioxidants; D018038:Sodium Selenite
keywords: Dietary selenium; Proliferation; Rooster; Spermatogonial stem cells