title: Lack of association between the MTHFRC677T polymorphism and lung cancer in a Turkish population.
abstract: BACKGROUND
  In this case-control study, we aimed to investigate the relationship between the methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism and lung cancer.
  
  
  METHODS
  Total 200 individuals including 100 patients with lung cancer and 100 controls were analyzed. Genotyping of MTHFR C677T was performed using PCR and RFLP methods.
  
  
  RESULTS
  The majority of the patients were men and 90% were smokers. We found that the risk ratio for development of LC was 13-times higher in smokers compared with non-smokers between patient and control groups in our study (OR:13.5, 95%CI:6.27-29.04, p:0.0001). Besides, the risk ratio for development of LC was nine times higher in individuals with cancer history in their family than those without cancer history (OR:9.65, 95%CI: 2.79-33.36; p:0.0001). When genotype distributions and allele frequencies were analyzed in the study groups, no significant difference was apparent (χ2:0.53, p=0.76). In addition, no correlation between genotypes of MTHFRC677T polymorphism and histological type of LC was found (χ2:0.99, p=0.60).
  
  
  CONCLUSIONS
  These results suggest that there was no association between the MTHFR C677T polymorphism and lung cancer in the Turkish population.
authors.affiliation: Department of Research Centre, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey E-mail : meralylmz@gmail.com.
authors.forename: Meral
authors.initials: M
authors.lastname: Yilmaz
authors.forename: Turgut
authors.initials: T
authors.lastname: Kacan
authors.forename: Ismail
authors.initials: I
authors.lastname: Sari
authors.forename: Saadettin
authors.initials: S
authors.lastname: Kilickap
mesh_terms: D000230:Adenocarcinoma / Q000453:epidemiology / Q000235:genetics / Q000556:secondary; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000453:epidemiology / Q000235:genetics / Q000556:secondary; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000235:genetics / Q000556:secondary; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008175:Lung Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011379:Prognosis; D012307:Risk Factors; D055752:Small Cell Lung Carcinoma / Q000453:epidemiology / Q000235:genetics / Q000556:secondary; D014421:Turkey / Q000453:epidemiology
chemical_list: C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2)