title: Purification, characterisation and protective effects of polysaccharides from alfalfa on hepatocytes.
abstract: The objective of this study was to determine the preliminary characteristics and protective effects of alfalfa polysaccharides (APS) on hepatocytes in vitro. The crude APS was purified by DEAE-cellulose and Sephadex G-100 chromatography, resulting in the four purified fractions: APS-1, APS-2, APS-3 and APS-4. The results indicated that APS-3 had higher carbohydrate and uronic acid contents and that APS-4 had a more complicated monosaccharide composition compared to the other purified fractions. The average molecular weights of APS-1, APS-2, APS-3 and APS-4 were 48,536, 6,221, 66,559 and 13,076 Da, respectively. Furthermore, APS (crude and its purified fractions) restored the activities of antioxidant enzymes and increased the total antioxidant capacity of hepatocytes subjected to H2O2-induced oxidative stress. Furthermore, APS treatment counteracted the increases in lactic dehydrogenase and malonaldehyde in the culture supernatant. These results clearly demonstrate that APS possesses a protective effect against oxidative injury in hepatocytes.
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: shaopu.1988@163.com.
authors.forename: Shaopu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: xiaofangd1124@sina.com.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Dong
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. Electronic address: majordan@163.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Ma
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: cymnongkeyuan@163.com.
authors.forename: Yaoming
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: tjm606@263.net.
authors.forename: Jianming
authors.initials: J
authors.lastname: Tong
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002478:Cells, Cultured; D002645:Chickens; D002853:Chromatography, Liquid / Q000379:methods; D003911:Dextrans; D004798:Enzymes / Q000378:metabolism; D005260:Female; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D000455:Medicago sativa / Q000737:chemistry*; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D018384:Oxidative Stress / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D014574:Uronic Acids
chemical_list: D000975:Antioxidants; D003911:Dextrans; D004798:Enzymes; D009005:Monosaccharides; D011134:Polysaccharides; D020011:Protective Agents; D014574:Uronic Acids; D008315:Malondialdehyde; C025614:sephadex; D007770:L-Lactate Dehydrogenase
keywords: Alfalfa polysaccharides; Antioxidant; Hepatocytes; Molecular weight; Monosaccharide analysis