title: Validation of an HPLC-MS/MS and wipe procedure for mitomycin C contamination.
abstract: A high-performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method was developed for the determination of mitomycin C, an anticancer drug, from contamination on various surfaces. Mitomycin C is often used in various forms of intraperitoneal chemotherapy, and operating room healthcare worker exposure to this drug is possible. The surface testing method consisted of a wiping procedure utilizing a solution of 20/45/35 (v/v/v) of acetonitrile-isopropanol-water made 0.01 M in ammonium citrate (apparent pH 7.0). The wipe solutions were analyzed by means of HPLC-MS/MS using a reversed-phase gradient system and electrospray ionization in positive ion mode with a triple-quadrupole MS detector. Accuracy and precision of this method were demonstrated by a series of recovery studies of both spiked solutions and extracted wipes from various surfaces (stainless steel, vinyl and Formica(®)) spiked with known levels of mitomycin C. Recoveries of spiked solutions containing the analyte demonstrate mean recoveries (accuracy) ranged from 93 to 105%. Precision as measured by the relative standard deviation (% RSD) of multiple samples (n= 10) at each concentration level demonstrated values of 7.5% or less. The recoveries from spiked surfaces varied from 30 to 99%. The limit of detection for this methodology is ∼2 ng/100 cm(2) equivalent surface area, and the limit of quantitation is ∼6 ng/100 cm(2).
authors.affiliation: U.S. Department of Health and Human Services, Centers for Disease Control, National Institute for Occupational Safety and Health, Division of Applied Research and Technology, Taft Laboratory C-23, 4676 Columbia Parkway, Cincinnati, OH 45226, USA cbhymer@cdc.gov.
authors.forename: Clayton
authors.initials: C
authors.lastname: B'Hymer
authors.affiliation: U.S. Department of Health and Human Services, Centers for Disease Control, National Institute for Occupational Safety and Health, Division of Applied Research and Technology, Taft Laboratory C-23, 4676 Columbia Parkway, Cincinnati, OH 45226, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Connor
authors.affiliation: U.S. Department of Health and Human Services, Centers for Disease Control, National Institute for Occupational Safety and Health, Division of Applied Research and Technology, Taft Laboratory C-23, 4676 Columbia Parkway, Cincinnati, OH 45226, USA.
authors.forename: Derek
authors.initials: D
authors.lastname: Stinson
authors.affiliation: U.S. Department of Health and Human Services, Centers for Disease Control, National Institute for Occupational Safety and Health, Division of Applied Research and Technology, Taft Laboratory C-23, 4676 Columbia Parkway, Cincinnati, OH 45226, USA.
authors.forename: Jack
authors.initials: J
authors.lastname: Pretty
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003666:Decontamination / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D016685:Mitomycin / Q000032:analysis*; D016273:Occupational Exposure / Q000517:prevention & control*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D016685:Mitomycin