title: Non-Structural protein 1 (NS1) gene of Canine Parvovirus-2 regresses chemically induced skin tumors in Wistar rats.
abstract: The Non-Structural protein 1 of Canine Parvovirus-2 (CPV2.NS1) plays a major role in viral cytotoxicity and pathogenicity. CPV2.NS1 has been proven to cause apoptosis in HeLa cells in vitro in our laboratory. Here we report that CPV2.NS1 has no toxic side effects on healthy cells but regresses skin tumors in Wistar rats. Histopathological examination of tumor tissue from CPV2.NS1 treated group revealed infiltration of mononuclear and polymorphonuclear cells with increased extra cellular matrix, indicating signs of regression. Tumor regression was also evidenced by significant decrease in mitotic index, AgNOR count and PCNA index, and increase in TUNEL positive apoptotic cells in CPV2.NS1 treated group. Further, CPV2.NS1 induced anti-tumor immune response through significant increase in CD8(+) and NK cell population in CPV2.NS1 treated group. These findings suggest that CPV2.NS1 can be a possible therapeutic candidate as an alternative to chemotherapy for the treatment of cancer.
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Lakshman
authors.initials: L
authors.lastname: Santra
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: R S
authors.initials: RS
authors.lastname: Rajmani
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: G V P P S Ravi
authors.initials: GV
authors.lastname: Kumar
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Shikha
authors.initials: S
authors.lastname: Saxena
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Sujoy K
authors.initials: SK
authors.lastname: Dhara
authors.affiliation: Division of Animal Genetics and Breeding/Laboratory Animal Resources, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Aditya Prasad
authors.initials: AP
authors.lastname: Sahoo
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Lakshya Veer
authors.initials: LV
authors.lastname: Singh
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: G S
authors.initials: GS
authors.lastname: Desai
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Uttara
authors.initials: U
authors.lastname: Chaturvedi
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
authors.forename: Sudesh
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India. Electronic address: aktiwari63@yahoo.com.
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Tiwari
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000009:adverse effects; D000818:Animals; D017209:Apoptosis; D002273:Carcinogens; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000628:therapy / Q000662:veterinary*; D004195:Disease Models, Animal; D004283:Dog Diseases / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D004285:Dogs; D015316:Genetic Therapy / Q000379:methods*; D008297:Male; D008940:Mitotic Index; D017993:Parvovirus, Canine / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D012878:Skin Neoplasms / Q000473:pathology / Q000628:therapy / Q000662:veterinary*; D016896:Treatment Outcome; D017361:Viral Nonstructural Proteins / Q000235:genetics*
chemical_list: D002273:Carcinogens; C106202:NS1 protein, parvovirus; D017361:Viral Nonstructural Proteins; D015127:9,10-Dimethyl-1,2-benzanthracene
keywords: Anti-tumor immunity; Canine Parvovirus-2 NS1 gene; DMBA; Squamous cell carcinoma; Tumor regression; Viral gene oncotherapy