title: Apigenin suppresses GLUT-1 and p-AKT expression to enhance the chemosensitivity to cisplatin of laryngeal carcinoma Hep-2 cells: an in vitro study.
abstract: Glucose transporter-1 (GLUT-1) and PI3K/Akt are known to be closely involved in resistance to chemotherapy. Co-targeted therapy reducing GLUT-1 expression and PI3K/Akt pathway activity may overcome the chemoresistance of human cancers. Apigenin may inhibit the expression of GLUT-1 and the PI3K/Akt pathway. We hypothesized that over-expression of GLUT-1 and p-Akt was associated with the resistance to cisplatin of laryngeal carcinoma Hep-2 cells. We explored whether apigenin inhibited GLUT-1 and p-Akt, resulting in sensitization of laryngeal carcinoma Hep-2 cells to cisplatin. Real-time RT-PCR and Western blotting confirmed the presence of GLUT-1 mRNA, and GLUT-1 and p-Akt proteins in Hep-2 cells. We found that resistance or insensitivity of Hep-2 cells to cisplatin might be associated with such expression. Apigenin markedly enhanced the cisplatin-induced suppression of Hep-2 cell growth. This effect was concentration- and time-dependent. Thus apigenin may significantly reduce the levels of GLUT-1 mRNA, and GLUT-1 and p-Akt proteins, in cisplatin-treated Hep-2 cells, in a concentration- and time-dependent manner. To conclude, overexpression of GLUT-1 mRNA may be associated with the resistance to cisplatin of laryngeal carcinoma Hep-2 cells. Apigenin may enhance the sensitivity to cisplatin of laryngeal carcinoma cells via inhibition of GLUT-1 and p-Akt expression.
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital, College of Medicine, Zhejiang University 310003, China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Xu
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital, College of Medicine, Zhejiang University 310003, China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Wu
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital, College of Medicine, Zhejiang University 310003, China.
authors.forename: Shui-Hong
authors.initials: SH
authors.lastname: Zhou
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital, College of Medicine, Zhejiang University 310003, China.
authors.forename: Yang-Yang
authors.initials: YY
authors.lastname: Bao
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital, College of Medicine, Zhejiang University 310003, China.
authors.forename: Qin-Ying
authors.initials: QY
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University 310003, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Fan
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University 310003, China.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Huang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D047310:Apigenin / Q000494:pharmacology*; D015153:Blotting, Western; D002277:Carcinoma / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007822:Laryngeal Neoplasms / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000970:Antineoplastic Agents; D051272:Glucose Transporter Type 1; D047310:Apigenin; D002945:Cisplatin
keywords: Glucose transporter-1; apigenin; chemosensitivity; cisplatin; laryngeal carcinoma; pi3k/akt