title: Bacteria aerosol spread and wound bacteria reduction with different methods for wound debridement in an animal model.
abstract: Debridement is essential in wound treatment to remove necrotic tissue and wound bacteria but may lead to bacteria spread by aerosolization. This study investigated the wound bacterial reduction and bacterial transmission induced by debridement using curette, plasma-mediated bipolar radiofrequency ablation (Coblation®) or hydrodebridement (Versajet®). Full thickness dermal wounds in porcine joint specimens inoculated with S. aureus were debrided with curette, Coblation, Versajet, or were left untreated. During and after debridement, aerosolized bacteria were measured and to assess wound bacterial load, quantitative swab samples were taken from each wound. Only Coblation was able to reduce the bacterial load of the wound significantly. Versajet debridement resulted in a significant bacterial aerosolization, but this was not the case with Coblation and curette debridement. This study shows that Coblation is a promising wound debridement method, which effectively reduces the wound bed bacterial load without the risk of bacterial aerosolization.
authors.affiliation: Department of Dermatology, Institute of Clinical Sciences, The Sahlgrenska Academy at University of Gothenburg, SE-413 45 Gothenburg, Sweden. henrik.sonnergren@gu.se.
authors.forename: Henrik H
authors.initials: HH
authors.lastname: Sönnergren
authors.forename: Sam
authors.initials: S
authors.lastname: Polesie
authors.forename: Louise
authors.initials: L
authors.lastname: Strömbeck
authors.forename: Frank
authors.initials: F
authors.lastname: Aldenborg
authors.forename: Bengt R
authors.initials: BR
authors.lastname: Johansson
authors.forename: Jan
authors.initials: J
authors.lastname: Faergemann
mesh_terms: D055011:Ablation Techniques / Q000009:adverse effects / Q000295:instrumentation; D000336:Aerosols; D000391:Air Microbiology; D000818:Animals; D058491:Bacterial Load; D018441:Biofilms / Q000254:growth & development; D003646:Debridement / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods*; D004195:Disease Models, Animal; D004867:Equipment Design; D018570:Risk Assessment; D013203:Staphylococcal Infections / Q000382:microbiology / Q000601:surgery* / Q000635:transmission; D013211:Staphylococcus aureus / Q000254:growth & development*; D013552:Swine; D007507:Therapeutic Irrigation / Q000009:adverse effects / Q000295:instrumentation; D013997:Time Factors; D014945:Wound Healing; D014946:Wound Infection / Q000382:microbiology / Q000601:surgery* / Q000635:transmission
chemical_list: D000336:Aerosols