title: Protective effects of escin against indomethacin-induced gastric ulcer in mice.
abstract: Escin, a natural mixture of triterpenoid saponin isolated from the seed of the horse chestnut, is reported to have a potent antiulcer activity against ethanol-induced gastric mucosal lesions. This study investigated the possible mechanisms underlying the gastroprotective effect of escin against indomethacin-induced gastric ulcer in mice. Gastric ulceration was induced by a single intragastric administration of indomethacin (18 mg/kg). The mice underwent intragastric treatment with escin at doses of 0.45, 0.9 or 1.8 mg/kg. Gastric lesion was estimated morphometrically and histopathologically 6 h after the indomethacin administration. The antioxidative parameters in gastric mucosa were measured. Moreover, the activity of myeloperoxidase and the contents of TNF-α, P-selectin and VCAM-1 in gastric tissues were determined. The results showed that escin protected gastric tissues against indomethacin-induced gastropathy as demonstrated from a reduction in the ulcer index and an attenuation of histopathologic changes. Escin caused significant reductions of the contents of malondialdehyde, TNF-α, P-selectin, VCAM-1 and myeloperoxidase activity. The altered activities of superoxide dismutase, catalase and glutathione peroxidase in the stomach tissues were also ameliorated by escin treatment. The present study demonstrated that escin had a protective effect against indomethacin-induced gastric ulcer in mice, not only by virtue of its antioxidant potential, but also due to its anti-inflammatory effect.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Yantai University , Yantai , PR China and.
authors.forename: Tian
authors.initials: T
authors.lastname: Wang
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhao
authors.forename: Yucun
authors.initials: Y
authors.lastname: Wang
authors.forename: Yujiao
authors.initials: Y
authors.lastname: Yang
authors.forename: Le
authors.initials: L
authors.lastname: Yao
authors.forename: Liuxiang
authors.initials: L
authors.lastname: Chu
authors.forename: Hanhan
authors.initials: H
authors.lastname: Du
authors.forename: Fenghua
authors.initials: F
authors.lastname: Fu
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000009:adverse effects / Q000037:antagonists & inhibitors* / Q000627:therapeutic use; D000897:Anti-Ulcer Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D004928:Escin / Q000008:administration & dosage / Q000627:therapeutic use*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D017506:H(+)-K(+)-Exchanging ATPase / Q000737:chemistry / Q000378:metabolism; D007213:Indomethacin / Q000009:adverse effects / Q000037:antagonists & inhibitors*; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism; D019007:P-Selectin / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D020011:Protective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D011897:Random Allocation; D012720:Severity of Illness Index; D013270:Stomach / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D013276:Stomach Ulcer / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000737:chemistry / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000897:Anti-Ulcer Agents; D000975:Antioxidants; D019007:P-Selectin; D020011:Protective Agents; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D004928:Escin; D010088:Oxidoreductases; D017506:H(+)-K(+)-Exchanging ATPase; D007213:Indomethacin
keywords: Anti-inflammatory; antioxidant; escin; gastric ulcer; indomethacin