title: Apoptosis of human tongue squamous cell carcinoma cell (CAL-27) induced by Lactobacillus sp. A-2 metabolites.
abstract: OBJECTIVE
  To study the effect of Lactobacillus sp. A-2 metabolites on viability of CAL-27 cells and apoptosis in CAL-27 cells.
  
  
  METHODS
  Lactobacillus sp. A-2 metabolites 1 and 2 (LM1 and LM2) were obtained by culturing Lactobacillus sp. A-2 in reconstituted whey medium and whey-inulin medium; the cultured CAL-27 cells were treated with different concentrations of LM1 and LM2 (0, 3, 6, 12, 24, 48 mg/mL) and assayed by methyl thiazolyltetrazolium (MTT) method; morphological changes of apoptotic cell were observed under fluorescence microscopy by acridine orange (Ao) fluorescent staining; flow cytometry method (FCM) and agarose gel electrophoresis were used to detect the apoptosis of CAL-27 cells treated LM1 and LM2.
  
  
  RESULTS
  The different concentrations of LM1 and LM2 could restrain the growth of CAL-27 cells, and in a dose-dependent manner; the apoptosis of CAL-27 cells was obviously induced and was time-dependent.
  
  
  CONCLUSIONS
  Viability of CAL-27 cells was inhibited by Lactobacillus sp. A-2 metabolites; Lactobacillus sp. A-2 metabolites could induce CAL-27 cells apoptosis; study on the bioactive compounds in the Lactobacillus sp. A-2 metabolites and their molecular mechanism is in progress.
authors.affiliation: Stomatological College, Jiamusi University, Jiamusi, Heilongjiang, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Pharmacy School, Qiqihaer Medical University, Qiqihaer, Heilongjiang, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Stomatological College, Jiamusi University, Jiamusi, Heilongjiang, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Research Center of Microecological Engineering Technology, Qiqihaer Medical University, Qiqihaer, Heilongjiang, China.
authors.forename: Wenqin
authors.initials: W
authors.lastname: Yang
authors.affiliation: Stomatological College, Jiamusi University, Jiamusi, Heilongjiang, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Stomatological College, Jiamusi University, Jiamusi, Heilongjiang, China.
authors.forename: Chaurasia Nitesh
authors.initials: CN
authors.lastname: Kumar
authors.affiliation: Research Center of Microecological Engineering Technology, Qiqihaer Medical University, Qiqihaer, Heilongjiang, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Guan
authors.affiliation: Stomatological College, Jiamusi University, Jiamusi, Heilongjiang, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Guan
mesh_terms: D000704:Analysis of Variance; D017209:Apoptosis / Q000502:physiology*; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002470:Cell Survival; D002478:Cells, Cultured; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D006801:Humans; D007778:Lactobacillus / Q000254:growth & development / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors; D014062:Tongue Neoplasms / Q000473:pathology*
chemical_list: D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue