title: Synthesis and surface modification of polyurethanes with chitosan for antibacterial properties.
abstract: Surface modification and providing antibacterial properties to the materials or devices are getting great attention especially in the last decades. In this study, polyurethane (PU) films were prepared by synthesizing them in medical purity from toluene diisocyanate and polypropylene ethylene glycol without using any other ingredients and then the film surfaces were modified by covalent immobilization of chitosan (CH) which has antibacterial activity. CH immobilized PU films (PU-CH) were found to be more hydrophilic than control PU films. Electron Spectroscopy for Chemical Analysis (ESCA) and Atomic Force Microscopy (AFM) analyses showed higher nitrogen contents and rougher surface topography for PU-CH compared to PU films. Modification with CH significantly increased antibacterial activity against Gram positive (Staphylococcus aureus) and Gram negative (Pseudomonas aeruginosa) bacteria. It was observed that the number of bacteria colonies were less about 10(2)-10(5) CFU/mL and number of attached viable bacteria decreased significantly after CH modification of PU films.
authors.affiliation: Department of Chemistry, Faculty of Science, Gazi University, 06500 Ankara, Turkey.
authors.forename: Filiz
authors.initials: F
authors.lastname: Kara
authors.affiliation: Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Hacettepe University, 06100 Ankara, Turkey; BIOMATEN, Center of Excellence in Biomaterials and Tissue Engineering, Middle East Technical University, Dumlupınar Bulvarı, No:1, 06800 Cankaya, Ankara, Turkey.
authors.forename: Eda Ayse
authors.initials: EA
authors.lastname: Aksoy
authors.affiliation: Department of Biology, Biotechnology Laboratory, Faculty of Science, Gazi University, 06500 Ankara, Turkey.
authors.forename: Zehranur
authors.initials: Z
authors.lastname: Yuksekdag
authors.affiliation: BIOMATEN, Center of Excellence in Biomaterials and Tissue Engineering, Middle East Technical University, Dumlupınar Bulvarı, No:1, 06800 Cankaya, Ankara, Turkey; Department of Chemistry, Faculty of Arts and Sciences, Middle East Technical University, 06800 Ankara, Turkey.
authors.forename: Nesrin
authors.initials: N
authors.lastname: Hasirci
authors.affiliation: Department of Chemistry, Faculty of Science, Gazi University, 06500 Ankara, Turkey. Electronic address: seraksoy@gazi.edu.tr.
authors.forename: Serpil
authors.initials: S
authors.lastname: Aksoy
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D018625:Microscopy, Atomic Force; D056951:Photoelectron Spectroscopy; D011140:Polyurethanes / Q000737:chemistry* / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D011140:Polyurethanes; D048271:Chitosan
keywords: Antibacterial; Bacterial adhesion; Chitosan; Polyurethane; Surface modification