title: Inhibition of N-acetylglucosaminyltransferase V enhances sensitivity of radiotherapy in human prostate cancer.
abstract: The purpose of this study was to investigate the relationship between N-acetylglucosaminyltransferase V (GnT-V) and radiation sensitivity of prostate cancer (PCa) cells both in vitro and in vivo. Firstly, the GnT-V expression was studied in 84 cases of PCa tissues, in which higher level of GnT-V was detected more frequently in the advanced tumors. Secondly, the GnT-V stably suppressed cell lines PCa/1079 (Lncap/1079 and PC3/1079) were constructed from PCa cell lines (Lncap and PC3) in vitro. Attenuation of GnT-V inhibited cell proliferation, migration and increased apoptosis, which resulted in enhanced radiation sensitivity of PCa cells. The underlying mechanism may be relevant to the increasing ratio of Bax/Bcl-2, the blocking transcription of NF-κB and the reduction of cell cycle G2-M arrest. Finally, in in vivo study, compared with control groups, the irradiated PCa xenograft nude mice of PCa/1079 indicated to reduce tumor-growth rate and enhance survival time. Summary, our studies showed that inhibition of GnT-V probably improved PCa cells' radiation sensitivity.
authors.affiliation: Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Huiyi
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Wenxia
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Oncology, First People's Hospital of Jiujiang City, Jiujiang 332000, China.
authors.forename: Qiulian
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wei
authors.affiliation: Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Weiliang
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Meng
authors.affiliation: Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Linlang
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. Electronic address: 13925091863@139.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D017351:N-Acetylglucosaminyltransferases / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D011471:Prostatic Neoplasms / Q000532:radiotherapy*; D011836:Radiation Tolerance / Q000187:drug effects* / Q000235:genetics
chemical_list: D017351:N-Acetylglucosaminyltransferases; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase
keywords: Apoptosis; N-acetylglucosaminyltransferases V; Prostate cancer; Radiation sensitivity