title: Serum M30 and M65 levels and effects of Ankaferd blood stopper in cerulein induced experimental acute pancreatitis model in rats.
abstract: OBJECTIVE
  The incidence of acute pancreatitis is increasing recently. The aim of this study is to investigate the effect of Ankaferd Blood Stopper (ABS) on experimental model of cerulein induced acute pancreatitis in rats.
  
  
  METHODS
  Forty Wistar Albino rats were divided into five groups. Group 1: Sham (n = 8), Group 2: Control group (n = 8), Group 3: Treatment group (n = 8), Group 4: Prophylaxis group (n = 8), Group 5: Prophylaxis treatment group (n = 8). Any practice was not administered to Group 1. Rats were treated with either 1 ml ABS or 1 ml saline via intraperitoneal route before and after inducing acute pancreatitis. Pancreatic tissues were examined histopathologically. Amylase, cytokines (tumor necrosis factor-α and interleukin-1β), and markers of apoptosis (M30 and M65) were also measured in blood samples. Immunohistochemical staining was performed with caspase 3 antibody.
  
  
  RESULTS
  We found a statistically significant improvement in histopathological scores in treatment group and prophylaxis group compared with controls. In treatment group, M30 and M65 levels were lower when compared with controls. In prophylaxis group, there was not a statistically significant difference in M30 levels, but M65 levels were lower when compared with controls.
  
  
  CONCLUSIONS
  In this experimental acute pancreatitis model, we found high histopathological healing effects of ABS treatment and also prophylaxis. ABS treatment and prophylaxis reduced apoptosis.
authors.affiliation: Department of Gastroenterology, Dışkapı Yıldırım Beyazıt Training and Research Hospital Ankara, Turkey.
authors.forename: Bora
authors.initials: B
authors.lastname: Aktaş
authors.affiliation: Department of Gastroenterology, Hacettepe Universty Medical School Ankara, Turkey.
authors.forename: Omer
authors.initials: O
authors.lastname: Başar
authors.affiliation: Department of Gastroenterology, Dışkapı Yıldırım Beyazıt Training and Research Hospital Ankara, Turkey.
authors.forename: Barış
authors.initials: B
authors.lastname: Yılmaz
authors.affiliation: Department of Gastroenterology, Dışkapı Yıldırım Beyazıt Training and Research Hospital Ankara, Turkey.
authors.forename: Fuat
authors.initials: F
authors.lastname: Ekiz
authors.affiliation: Department of Gastroenterology, Dışkapı Yıldırım Beyazıt Training and Research Hospital Ankara, Turkey.
authors.forename: Akif
authors.initials: A
authors.lastname: Altınbaş
authors.affiliation: Department of Gastroenterology, Dışkapı Yıldırım Beyazıt Training and Research Hospital Ankara, Turkey.
authors.forename: Sahin
authors.initials: S
authors.lastname: Coban
authors.affiliation: Department of Emergency Medicine, Ankara Dışkapı Yıldırım Beyazıt Training and Research Hospital Ankara, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Büyükçam
authors.affiliation: Department of Pathology, Dışkapı Yıldırım Beyazıt Training and Research Hospital Ankara, Turkey.
authors.forename: Aynur
authors.initials: A
authors.lastname: Albayrak
authors.affiliation: Department of Biochemistry, Ankara Dışkapı Yıldırım Beyazıt Training and Research Hospital Ankara, Turkey.
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Giniş
authors.affiliation: Department of Gastroenterology, Hacettepe Universty Medical School Ankara, Turkey.
authors.forename: Osman
authors.initials: O
authors.lastname: Yüksel
authors.affiliation: Department of Endocrinology, Hacettepe Universty Medical School Ankara, Turkey.
authors.forename: Tuncay
authors.initials: T
authors.lastname: Delibaşı
keywords: Ankaferd blood stopper; animal models; apoptosis; experimental acute pancreatitis