title: [Targeted inhibition of WISP1 enhanced radiosensitivity in glioma cells].
abstract: OBJECTIVE
  To explore the effects of differencial expression of WISP1 upon the radiosenstivity and migration in glioma cell lines M059K and M059J, as well as WISP1 knockdown cell line M059K-WISP1-.
  
  
  METHODS
  The expression of WISP1 in different cell lines was detected by Western blot assay. The lentivirus mediated-RNA interference technology was employed to knockdown the endogenous expression of gene WISP1 in human glioma cells(M059K). A stable cell line was established by infecting M059K cells with the lentivirus particles, the transfection efficiency was examined by fluorescence microscopy. The radiosensitivity and migration ability of cells were assessed by Colony-forming and Scratch Wound Assays respectively.
  
  
  RESULTS
  WISP1 was highly expressed in M059K cells compared with M059J cells. M059K cells were less radiosensitive than M059J cells , and had a better ability of migration. M059K cells were transfected into by the lentiviral vector pGLV3/H1/GFP+puro vector -WISP1. And M059K-WISP1- cell line with stable WISP1 downregulation was established successfully. Western blot demonstrated significantly downregulated WISP1 expression in M059K-WISP1- cells, which showed obviously improved radiosensitivity in Colony-forming assay and suppressed migration ability in Scratch Wound Assay. Colony-forming assay based on the muti-target/single-hit model indicated that radiosensitivity of M059K-WISP1- was significantly decreased.
  
  
  CONCLUSIONS
  Compared with WISP1 low expression cell line M059J , WISP1 high expression cell line M059K was less radiosensitive and had a better ability of migration.We have successfully established a glioma cell line M059K-WISP1- stably downregulating WISP1, which shows a better radiosensitivity and a lowered migration activity in vitro. This may provide a novel therapeutic target for the treatment of human glioma.
authors.affiliation: Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
authors.forename: Tao
authors.initials: T
authors.lastname: Song
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Wang
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Jiang
authors.forename: Hongfang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Email: wushixiu@medmail.com.cn.
authors.forename: Shixiu
authors.initials: S
authors.lastname: Wu
mesh_terms: D055511:CCN Intercellular Signaling Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D055785:Gene Knockdown Techniques; D005822:Genetic Vectors; D005910:Glioma / Q000473:pathology*; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D011836:Radiation Tolerance / Q000235:genetics*; D014162:Transfection
chemical_list: D055511:CCN Intercellular Signaling Proteins; C116396:CCN4 protein, human; D011518:Proto-Oncogene Proteins