title: Decreased expression of elastin and lysyl oxidase family genes in urogenital tissues of aging mice.
abstract: OBJECTIVE
  This study aims to investigate the expression levels of elastin and lysyl oxidase (LOX) family members in the urogenital tissues of natural aging mice and accelerated ovarian aging mice.
  
  
  METHODS
  Uteri, vaginas and bladders were harvested from 18-month-old female mice and accelerated ovarian aging mice developed by chemotherapeutic agents. Untreated 3-month-old female mice were used as controls. The expression levels of elastin and LOX family members were determined by real-time polymerase chain reaction and western blot.
  
  
  RESULTS
  Compared with untreated young female mice, the expression of elastin and LOX family members significantly decreased both in natural aging mice and accelerated ovarian aging mice.
  
  
  CONCLUSIONS
  Aging is a high-risk factor for pelvic floor disorders. The failure of elastic fiber synthesis and assembly due to the decline in expression levels of elastin and LOX family members during aging may explain the molecular mechanism causing pelvic floor disorders.
authors.affiliation: Key Laboratory of the Ministry of Education for Experimental Teratology, School of Life Science, Shandong University, Jinan, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Wen
authors.initials: W
authors.lastname: Zong
authors.forename: Hong
authors.initials: H
authors.lastname: Luan
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Liu
authors.forename: Ai-zhen
authors.initials: AZ
authors.lastname: Zhang
authors.forename: Xiao-lin
authors.initials: XL
authors.lastname: Li
authors.forename: Shuo-yang
authors.initials: SY
authors.lastname: Liu
authors.forename: Shi-qian
authors.initials: SQ
authors.lastname: Zhang
authors.forename: Jian-gang
authors.initials: JG
authors.lastname: Gao
mesh_terms: D000375:Aging; D000818:Animals; D015536:Down-Regulation; D004549:Elastin / Q000235:genetics / Q000378:metabolism*; D005260:Female; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008815:Mice, Inbred Strains; D008249:Protein-Lysine 6-Oxidase / Q000235:genetics / Q000378:metabolism*; D001743:Urinary Bladder / Q000254:growth & development / Q000378:metabolism*; D014599:Uterus / Q000254:growth & development / Q000378:metabolism*; D014621:Vagina / Q000254:growth & development / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D004549:Elastin; D008249:Protein-Lysine 6-Oxidase
keywords: elastin; lysyl oxidase family; pelvic floor disorders