title: Type V collagen counteracts osteo-differentiation of human mesenchymal stem cells.
abstract: In search of novel gene signatures for osteo-differentiation of mesenchymal stem cells (MSCs), we submitted cDNA preparations from undifferentiated and differentiating MSCs to differential display- and semiquantitative-PCR and found down-regulation of COL5A1 in osteo-induced cultures at days 21 and 28, when the mineralized matrix accumulates. We also cultured osteo-differentiating MSCs onto type V collagen substrates and found a decrease in the accumulation of extracellular calcium compared to those grown in uncoated flasks. To our knowledge, this is first evidence that type V collagen might represent a stromal component that impairs osteogenesis.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), Università di Palermo, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Longo
authors.affiliation: Department of Natural Sciences, Bonn-Rhein-Sieg University, Rheinbach, Germany.
authors.forename: Edda
authors.initials: E
authors.lastname: Tobiasch
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), Università di Palermo, Italy. Electronic address: claudio.luparello@unipa.it.
authors.forename: Claudio
authors.initials: C
authors.lastname: Luparello
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D024062:Collagen Type V / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics* / Q000502:physiology*
chemical_list: C530725:COL5A1 protein, human; D024062:Collagen Type V
keywords: Collagen; Gene expression; Osteogenesis; Stem cells