title: Applicable apparent diffusion coefficient of an orthotopic mouse model of gastric cancer by improved clinical MRI diffusion weighted imaging.
abstract: In vivo imaging studies in animal models are hindered by variables that contribute to poor image quality and measurement reliability. As such we sought to improve the diffusion coefficient (ADC) of an orthotopic mouse model of gastric cancer in diffusion-weighted images (DWI) using alginate moulding and Ultrasonic coupling medium. BGC-823 human gastric cancer cells were subcutaneously injected into the abdomen of nude mice and 1 mm(3) primary tumour was orthotopically transplanted. Alginate and coupling medium were applied to the mice and MRI (T2 and DWI) was performed for 6 weeks. Regions of interest (ROI) were drawn and liver and tumour ADC were evaluated. Using alginate moulding, the mean quality total score of DW imaging was 8.53; however, in control animals this value was 5.20 (p < 0.001). The coefficient of variation of ADC of liver in experimental and control groups were 0.071 and 0.270 (p < 0.001), respectively, suggesting this method may be helpful for DWI studies of important human diseases such as gastric cancer.
authors.affiliation: Department of Radiology; Beijing Chao-Yang Hospital, 8 Gongren Tiyuchang Nanlu, Chaoyang District, Beijing, 100020, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Sun
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiology, Peking University Cancer Hospital &Institute. No.52, Fucheng Road, Haidian District, Beijing, 100142, China.
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiology, Peking University Cancer Hospital &Institute. No.52, Fucheng Road, Haidian District, Beijing, 100142, China.
authors.forename: Xiao-Ting
authors.initials: XT
authors.lastname: Li
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiology, Peking University Cancer Hospital &Institute. No.52, Fucheng Road, Haidian District, Beijing, 100142, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Tang
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiology, Peking University Cancer Hospital &Institute. No.52, Fucheng Road, Haidian District, Beijing, 100142, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiology, Peking University Cancer Hospital &Institute. No.52, Fucheng Road, Haidian District, Beijing, 100142, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiology, Peking University Cancer Hospital &Institute. No.52, Fucheng Road, Haidian District, Beijing, 100142, China.
authors.forename: Ying-Shi
authors.initials: YS
authors.lastname: Sun
mesh_terms: D000464:Alginates; D000818:Animals; D016477:Artifacts; D045744:Cell Line, Tumor; D003287:Contrast Media; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007089:Image Enhancement; D007103:Immobilization / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011859:Radiography; D015203:Reproducibility of Results; D013274:Stomach Neoplasms / Q000000981:diagnostic imaging*; D014183:Transplantation, Heterologous
chemical_list: D000464:Alginates; D003287:Contrast Media