title: miR-338-3p is over-expressed in blood, CFS, serum and spinal cord from sporadic amyotrophic lateral sclerosis patients.
abstract: Amyotrophic lateral sclerosis (ALS) is a progressive and seriously disabling adult-onset neurological disease. Ninety percent of ALS patients are sporadic cases (sALS) with no clear genetic linkage. Accumulating evidence indicates that various microRNAs (miRNAs), expressed in a spatially and temporally controlled manner in the brain, play a key role in neuronal development. In addition, microRNA dysregulation contributes to some mental disorders and neurodegeneration diseases. In our research, the expression of one selected miRNA, miR-338-3p, which previously we have found over-expressed in blood leukocytes, was studied in several different tissues from sALS patients. For the first time, we detected a specific microRNA disease-related upregulation, miR-338-3p, in blood leukocytes as well in cerebrospinal fluid, serum, and spinal cord from sALS patients. Besides, staining of in situ hybridization showed that the signals of miR-338-3p were localized in the grey matter of spinal cord tissues from sALS autopsied patients. We propose that miRNA profiles found in tissue samples from sALS patients can be relevant to understand sALS pathogenesis and lead to set up effective biomarkers for sALS early diagnosis.
authors.affiliation: DISTABIF-Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Naples II, Via Vivaldi 43, 81100, Caserta, Italy, bruna.defelice@unina2.it.
authors.forename: Bruna
authors.initials: B
authors.lastname: De Felice
authors.forename: Anna
authors.initials: A
authors.lastname: Annunziata
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Fiorentino
authors.forename: Marco
authors.initials: M
authors.lastname: Borra
authors.forename: Elio
authors.initials: E
authors.lastname: Biffali
authors.forename: Cinzia
authors.initials: C
authors.lastname: Coppola
authors.forename: Roberto
authors.initials: R
authors.lastname: Cotrufo
authors.forename: Johannes
authors.initials: J
authors.lastname: Brettschneider
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Giordana
authors.forename: Tamas
authors.initials: T
authors.lastname: Dalmay
authors.forename: Guy
authors.initials: G
authors.lastname: Wheeler
authors.forename: Raffaella
authors.initials: R
authors.lastname: D'Alessandro
mesh_terms: D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D008875:Middle Aged; D019636:Neurodegenerative Diseases / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C532050:MIRN338 microRNA, human; D035683:MicroRNAs