title: Telomerase Cajal body protein 1 depletion inhibits telomerase trafficking to telomeres and induces G1cell cycle arrest in A549 cells.
abstract: Telomerase Cajal body protein 1 (TCAB1) is a telomerase holoenzyme, which is markedly enriched in Cajal bodies (CBs) and facilitates the recruitment of telomerase to CBs in the S phase of the cell cycle. This recruitment is dependent on TCAB1 binding to a telomerase RNA component. The majority of cancer cells are able to grow indefinitely due to telomerase and its mechanism of trafficking to telomeres. In the present study, a certain level of TCAB1 expression in A549 human lung cells was identified and TCAB1 knockdown exhibited a potent antiproliferative effect on these cells, which was coupled with a decrease in the cell density and activity of the cellular enzymes. In addition, TCAB1-depletion was demonstrated to inhibit telomerase trafficking to telomeres in the A549 cells, leading to subsequent G1cell cycle arrest without inducing apoptotic cell death. Overall, these observations indicated that TCAB1 may be essential for A549 cell proliferation and cell cycle regulation, and may be a potential candidate for the development of a therapeutic target for lung adenocarcinomas.
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yuan
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
authors.forename: Zhitian
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
authors.forename: Wang
authors.initials: W
authors.lastname: Lv
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Pan
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
authors.forename: Yunhai
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
authors.forename: Xiaoshuai
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Hu
keywords: antiproliferation; cell cycle arrest; telomerase Cajal body protein 1; telomerase trafficking