title: [Holstein-Friesian RBC as human blood substitute].
abstract: α-Gal, the main xenotransplantation antigen, can lead to hyperacute rejection (HAR) in xenotransplantation. This study was purposed to investigate the effect of recombinant α-galactosidase (α-Gal antigen) on the Holstein-Friesian(H-F) red blood cells (RBC). The enzymelysis method was used to digest the α-Gal antigen on H-F RBC; the saline and anti-human globulin methods were used to perform the agglutination test of H-F RBC and human plasma; the flow cytometry was used to detect the α-Gal antigen on surface of H-F RBC, fluorescence intensity of FITC-IB4 and FITC-IgG labeled RBC. The results indicated that the saline and anti-human globulin method showed α-galactosidase-treated H-F RBC fail to agglutinate with human pooled plasma; the flow cytometry showed the fluorescence intensity of FITC-IB4 and FITC-IgG labeled RBC decrease 99.0% and 87.8%, respectively. It is concluded that the novel α-galactosidase can be used to cleared the α-Gal antigen on the surface of H-F RBC and α-galactosidase-treated H-F RBC may be considered as human blood substitute.
authors.affiliation: Shaanxi Blood Center, Xi'an 710061,Shaanxi Province, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Chen
authors.affiliation: Department of Blood Molecular Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China.
authors.forename: Su-Bo
authors.initials: SB
authors.lastname: Li
authors.affiliation: Department of Blood Molecular Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Blood Molecular Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gong
authors.affiliation: Department of Blood Molecular Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China. E-mail: gaohongwei1976@126.com.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Gao
authors.affiliation: Shaanxi Blood Center, Xi'an 710061,Shaanxi Province, China. E-mail: drxuhua@163.com.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Xu
mesh_terms: D000818:Animals; D001802:Blood Substitutes; D002417:Cattle; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D006801:Humans; D014183:Transplantation, Heterologous
chemical_list: D001802:Blood Substitutes