title: Parallel microdevice for high throughput analysis of levofloxacin using tris (2,2'-bipyridyl) ruthenium (II) and peroxydisulfate chemiluminescence system.
abstract: A parallel microdevice has been developed for high throughput analysis using microfluidics. The detection method is based on a chemiluminescence (CL) system based on the oxidation of tris (2,2'-bipyridyl) Ru(ll) [Ru (bipy)3(2+)] by peroxydisulfate. The device consists of a photoreactor chip and two detection chips. The sample throughput can reach up to 720 runs/h with the total reagent consumption of only 2.4 mL. The parallel microdevice was evaluated using levofloxacin (LEVO) in pharmaceutical preparations. The various factors that affect the CL signal were optimized, and the LOD was found to be 30 and 27 microg/L for the two detectors, respectively (S/N = 3), while RSD was 1.1% (n = 15) for 1.0 mg/L LEVO. Two tablet samples that contain LEVO as an active ingredient were successfully analyzed using the proposed parallel microdevice.
authors.forename: Haider A J
authors.initials: HA
authors.lastname: Al Lawati
authors.forename: Eiman
authors.initials: E
authors.lastname: Al Gharibi
authors.forename: Salma M Z
authors.initials: SM
authors.lastname: Al Kindy
authors.forename: Fakhr Eldin O
authors.initials: FE
authors.lastname: Suliman
authors.forename: Ali M
authors.initials: AM
authors.lastname: Al-Lawati
mesh_terms: D064704:Levofloxacin / Q000032:analysis*; D008163:Luminescent Measurements / Q000295:instrumentation; D046210:Microfluidic Analytical Techniques; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry*
chemical_list: D009942:Organometallic Compounds; D013431:Sulfates; C415768:tris(2,2'-bipyridyl)ruthenium(II); D064704:Levofloxacin