title: Mycoplasma hyorhinis infection promotes NF-κB-dependent migration of gastric cancer cells.
abstract: Chronic infection of Mycoplasma hyorhinis (M. hyorhinis) has been postulated to be associated with several types of cancer, but its effect on patients' survival and host factors mediating its infection remain unclear. Herein, we demonstrated that M. hyorhinis p37 protein expression in gastric cancer tissues predicts poor survival and associates with metastasis. M. hyorhinis infects mammalian cells and promotes gastric cancer cell invasiveness via its membrane protein p37. Synthesized peptide corresponding to the N-terminus of p37 prevents M. hyorhinis infection. Host Annexin A2 (ANXA2) interacts with the N-terminus of p37. In addition, EGFR forms a complex with p37 and ANXA2, and is required for M. hyorhinis-induced phosphorylation and membrane recruitment of ANXA2. M. hyorhinis infection is inhibited by siRNA-mediated knockdown of ANXA2 or EGFR, but is enhanced by expression of ectopic ANXA2 or EGFR. Downstream of ANXA2 and EGFR, the NF-κB pathway is activated and mediates M. hyorhinis-driven cell migration. In conclusion, our study unveils the effect of M. hyorhinis infection on gastric cancer survival and uncovers the mechanisms by which M. hyorhinis infects mammalian cells and promotes cancer cell migration.
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, Peking University Cancer Hospital and Institute, Beijing, China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Duan
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, Peking University Cancer Hospital and Institute, Beijing, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, Peking University Cancer Hospital and Institute, Beijing, China.
authors.forename: Like
authors.initials: L
authors.lastname: Qu
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, Peking University Cancer Hospital and Institute, Beijing, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, Peking University Cancer Hospital and Institute, Beijing, China.
authors.forename: Sonya Wei
authors.initials: SW
authors.lastname: Song
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, Peking University Cancer Hospital and Institute, Beijing, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Pathology, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, China.
authors.forename: Meihua
authors.initials: M
authors.lastname: Ye
authors.affiliation: Department of Pathology, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, China.
authors.forename: Wanyuan
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Pathology, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, China. shouchengchao@gmail.com xianglh992004@163.com.
authors.forename: Xianglei
authors.initials: X
authors.lastname: He
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, Peking University Cancer Hospital and Institute, Beijing, China. shouchengchao@gmail.com xianglh992004@163.com.
authors.forename: Chengchao
authors.initials: C
authors.lastname: Shou
mesh_terms: D017306:Annexin A2 / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D066246:ErbB Receptors / Q000378:metabolism; D005260:Female; D054884:Host-Pathogen Interactions; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009175:Mycoplasma Infections / Q000378:metabolism* / Q000473:pathology; D045790:Mycoplasma hyorhinis / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism*; D011485:Protein Binding; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000378:metabolism / Q000382:microbiology* / Q000401:mortality / Q000473:pathology
chemical_list: C472822:ANXA2 protein, human; D017306:Annexin A2; D001425:Bacterial Outer Membrane Proteins; D016328:NF-kappa B; C512478:EGFR protein, human; D066246:ErbB Receptors