title: In vitro fermentation of the polysaccharides from Cyclocarya paliurus leaves by human fecal inoculums.
abstract: In vitro fermentation of polysaccharide from Cyclocarya paliurus leaves by human fecal inoculums was investigated by determining the changes in contents of neutral and reducing sugar and pH value, consumption of monosaccharide and production of short-chain fatty acids (SCFAs). During fermentation, the content of neutral sugar and reducing sugar decreased as fermentation time increased except that the content of reducing sugar increased within the fermentation time 0.5h. The pH value significantly dropped from 7.2 to 6.04. Remarkably, the greatest yields and the fastest consumption of galacturonic acid were found and the yield of glucose and arabinose were relatively high. The dominant SCFAs, which were acetic acid, propionic acid and n-butyric acid, significantly increased. These results showed that polysaccharide was partly fermented, glycosidic bonds with galacturonic acid being more susceptible to be attacked by gut bacteria and galacturonic acid might be deemed as the main producer of acetic acid.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang 330047, Jiangxi, China.
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Min
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang 330047, Jiangxi, China.
authors.forename: Jie-Lun
authors.initials: JL
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang 330047, Jiangxi, China. Electronic address: spnie@ncu.edu.cn.
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Nie
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang 330047, Jiangxi, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang 330047, Jiangxi, China.
authors.forename: Ming-Yong
authors.initials: MY
authors.lastname: Xie
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D001089:Arabinose / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000032:analysis / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D005285:Fermentation; D006603:Hexuronic Acids / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D029525:Juglandaceae / Q000737:chemistry*; D018515:Plant Leaves / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism*; D011422:Propionates / Q000378:metabolism
chemical_list: D005232:Fatty Acids, Volatile; D006603:Hexuronic Acids; D011134:Polysaccharides; D011422:Propionates; C007819:galacturonic acid; D001089:Arabinose; C029658:propionic acid; D019342:Acetic Acid
keywords: Cyclocarya paliurus; In vitro fermentation; Monosaccharide consumption; Polysaccharide; Short-chain fatty acid