title: Preparation of biointeractive glycoprotein-conjugated hydrogels through metabolic oligosacchalide engineering.
abstract: In the current study, synthetic hydrogels containing metabolically engineered glycoproteins of mammalian cells were prepared for the first time and selectin-mediated cell adhesion on the hydrogel was demonstrated. A culture of HL-60 cells was supplemented with an appropriate volume of aqueous solution of N-methacryloyl mannosamine (ManMA) to give a final concentration of 5 mM. The cells were then incubated for 3 days to deliver methacryloyl groups to the glycoproteins of the cells. A transparent hydrogel was formed via redox radical polymerization of methacryloyl functionalized glycoproteins with 2-methacryloyloxyethyl phosphorylcholine and a cross-linker. Conjugation of the glycoproteins into the hydrogel was determined using Coomassie brilliant blue (CBB) and periodic acid-Schiff (PAS) staining. The surface density of P-selectin glycoprotein ligand-1 (PSGL-1) on the hydrogels was also detected using gold-colloid-labeled immunoassay. Finally, selectin-mediated cell adhesion on hydrogels containing glycoproteins was demonstrated. Selectin-mediated cell adhesion is considered an essential step in the progression of various diseases; therefore, hydrogels having glycoproteins could be useful in therapeutic and diagnostic applications.
authors.affiliation: Department of Chemistry and Materials Engineering Faculty of Chemistry, Materials and Bioengineering, Kansai University , 3-3-35 Yamate-cho, Suita-shi, Osaka 564-8680, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: Aki
authors.initials: A
authors.lastname: Matsunaga
authors.forename: Shuetsu
authors.initials: S
authors.lastname: Fujii
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D018922:HL-60 Cells; D006595:Hexosamines / Q000737:chemistry; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism; D060847:Metabolic Engineering; D009844:Oligosaccharides / Q000378:metabolism*; D019007:P-Selectin / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D006595:Hexosamines; D020100:Hydrogels; D009844:Oligosaccharides; D019007:P-Selectin; C008322:mannosamine