title: Novel porcine-like human G26P[19] rotavirus identified in hospitalized paediatric diarrhoea patients in Ho Chi Minh City, Vietnam.
abstract: During a hospital-based diarrhoeal disease study conducted in Ho Chi Minh City, Vietnam from 2009 to 2010, we identified four symptomatic children infected with G26P[19] rotavirus (RV)--an atypical variant that has not previously been reported in human gastroenteritis. To determine the genetic structure and investigate the origin of this G26P[19] strain, the whole genome of a representative example was characterized, revealing a novel genome constellation: G26-P[19]-I5-R1-C1-M1-A8-N1-T1-E1-H1. The genome segments were most closely related to porcine (VP7, VP4, VP6 and NSP1) and Wa-like porcine RVs (VP1-3 and NSP2-5). We proposed that this G26P[19] strain was the product of zoonotic transmission coupled with one or more reassortment events occurring in human and/or animal reservoirs. The identification of such strains has potential implications for vaccine efficacy in south-east Asia, and outlines the utility of whole-genome sequencing for studying RV diversity and zoonotic potential during disease surveillance.
authors.affiliation: The Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.|The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.
authors.forename: Phan Vu Tra
authors.initials: PVT
authors.lastname: My
authors.affiliation: Centre for Immunity, Infection and Evolution, University of Edinburgh, Edinburgh, UK.|The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.
authors.forename: Maia A
authors.initials: MA
authors.lastname: Rabaa
authors.affiliation: La Trobe University, Melbourne, Australia.|Murdoch Childrens Research Institute, Melbourne, Australia.
authors.forename: Celeste
authors.initials: C
authors.lastname: Donato
authors.affiliation: Murdoch Childrens Research Institute, Melbourne, Australia.
authors.forename: Daniel
authors.initials: D
authors.lastname: Cowley
authors.affiliation: The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.
authors.forename: Voong Vinh
authors.initials: VV
authors.lastname: Phat
authors.affiliation: The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.
authors.forename: Tran Thi Ngoc
authors.initials: TTN
authors.lastname: Dung
authors.affiliation: The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.
authors.forename: Pham Hong
authors.initials: PH
authors.lastname: Anh
authors.affiliation: The Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
authors.forename: Ha
authors.initials: H
authors.lastname: Vinh
authors.affiliation: Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Hanoi, Vietnam.
authors.forename: Juliet E
authors.initials: JE
authors.lastname: Bryant
authors.affiliation: Division of Infection and Immunity, University College London, London, United Kingdom.|The Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Kellam
authors.affiliation: Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, Oxford University, Oxford, UK.|The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.
authors.forename: Guy
authors.initials: G
authors.lastname: Thwaites
authors.affiliation: Centre for Immunity, Infection and Evolution, University of Edinburgh, Edinburgh, UK.
authors.forename: Mark E J
authors.initials: MEJ
authors.lastname: Woolhouse
authors.affiliation: La Trobe University, Melbourne, Australia.|Murdoch Childrens Research Institute, Melbourne, Australia.
authors.forename: Carl D
authors.initials: CD
authors.lastname: Kirkwood
authors.affiliation: The London School of Hygiene and Tropical Medicine, London, UK.|Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, Oxford University, Oxford, UK.|The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.
authors.forename: Stephen
authors.initials: S
authors.lastname: Baker
mesh_terms: D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006801:Humans; D007297:Inpatients; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*; D014744:Vietnam / Q000453:epidemiology