title: Multiscale patterned transplantable stem cell patches for bone tissue regeneration.
abstract: Stem cell-based therapy has been proposed as an enabling alternative not only for the treatment of diseases but also for the regeneration of tissues beyond complex surgical treatments or tissue transplantation. In this study, we approached a conceptual platform that can integrate stem cells into a multiscale patterned substrate for bone regeneration. Inspired by human bone tissue, we developed hierarchically micro- and nanopatterned transplantable patches as synthetic extracellular matrices by employing capillary force lithography in combination with a surface micro-wrinkling method using a poly(lactic-co-glycolic acid) (PLGA) polymer. The multiscale patterned PLGA patches were highly flexible and showed higher tissue adhesion to the underlying tissue than did the single nanopatterned patches. In response to the anisotropically multiscale patterned topography, the adhesion and differentiation of human mesenchymal stem cells (hMSCs) were sensitively controlled. Furthermore, the stem cell patch composed of hMSCs and transplantable PLGA substrate promoted bone regeneration in vivo when both the micro- and nanotopography of the substrate surfaces were synergistically combined. Thus, our study concludes that multiscale patterned transplantable stem cell patches may have a great potential for bone regeneration as well as for various regenerative medicine approaches.
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Jangho
authors.initials: J
authors.lastname: Kim
authors.affiliation: School of Mechanical and Aerospace Engineering, Seoul National University, Seoul 301-1202, Republic of Korea; Interdisciplinary Program of Bioengineering, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Won-Gyu
authors.initials: WG
authors.lastname: Bae
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul 110-749, Republic of Korea.
authors.forename: Han-Wool
authors.initials: HW
authors.lastname: Choung
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Ki Taek
authors.initials: KT
authors.lastname: Lim
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Hoon
authors.initials: H
authors.lastname: Seonwoo
authors.affiliation: School of Mechanical and Advanced Materials Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 689-798, Republic of Korea.
authors.forename: Hoon Eui
authors.initials: HE
authors.lastname: Jeong
authors.affiliation: School of Mechanical and Aerospace Engineering, Seoul National University, Seoul 301-1202, Republic of Korea; Interdisciplinary Program of Bioengineering, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Khap-Yang
authors.initials: KY
authors.lastname: Suh
authors.affiliation: School of Mechanical and Aerospace Engineering, Seoul National University, Seoul 301-1202, Republic of Korea; Interdisciplinary Program of Bioengineering, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Noo Li
authors.initials: NL
authors.lastname: Jeon
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul 110-749, Republic of Korea. Electronic address: choungph@snu.ac.kr.
authors.forename: Pill-Hoon
authors.initials: PH
authors.lastname: Choung
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, Republic of Korea; Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea. Electronic address: jchung@snu.ac.kr.
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Chung
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001861:Bone Regeneration / Q000502:physiology*; D001842:Bone and Bones; D002454:Cell Differentiation; D049109:Cell Proliferation; D005109:Extracellular Matrix / Q000737:chemistry; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: Bone regeneration; Hierarchical structures; Multiscale topography; Stem cell patch; Tissue engineering