title: Association of -1082 interleukin-10 gene polymorphism in Peruvian adults with chronic periodontitis.
abstract: OBJECTIVE
  The aim of this study was to assess association of the -1082 IL-10 gene polymorphism with chronic periodontitis CP in a Peruvian population.
  
  
  METHODS
  Samples of venous blood and DNA were obtained from 106 Peruvian subjects: a) 53 periodontally healthy; and b) 53 with CP. The association of the -1082 IL-10 promoter sequences was assessed by Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Student's t test were used to assess the clinical parameters, as well as the χ2 test and the odds ratio (OR), with 95% confidence intervals (CI) used performed for estimates regarding genotype and allele frequencies.
  
  
  RESULTS
  There were statistically significant differences between groups regarding the mean bleeding on probing, mean attachment level and mean probing depth (p < 0.00001) indicating that the matching based on the evaluated groups was adequate. The χ2 test found a statistically significant imbalance of genotypes between groups (p = 0.0172). The prevalence of CP was significantly higher in subjects harboring at least one A allele at position -1082 (AA and GA genotypes) in comparison to patients with the GG genotype (OR = 2.96; CI: 0.52; 5.41; p = 0.0099). Equally, subjects with the AA genotype were significantly associated to a diagnosis of CP (OR = 2.71; CI: 0.38; 5.04; p = 0.0231). On the other hand, subjects presenting a healthy periodontal status presented at least one G allele in comparison with the AA genotype (OR = 0.37; CI: 0.05, 0.69; p = 0.0231). For subjects with the GG genotype, the same positive association was observed (OR = 0.34; CI: 0.06, 0.62; p = 0.0099). There were no significant differences between groups amongst subjects with the GA genotype (OR = 1.19; CI: 0.22, 2.16; p = 0.6774).
  
  
  CONCLUSIONS
  Within the limits of this study, IL-10 gene polymorphism at position -1082 does not appear to be associated to CP. Conversely, subjects with AA genotype seem to be at an increased risk of developing CP.
authors.affiliation: Department of Periodontology - School of Dentistry, Científica del Sur University, Av. Paseo de la República N 5544, Lima 18 Peru, mega43@hotmail.com.
authors.forename: Leandro
authors.initials: L
authors.lastname: Chambrone
authors.forename: Amilcar
authors.initials: A
authors.lastname: Ascarza
authors.forename: Maria-Eugenia
authors.initials: ME
authors.lastname: Guerrero
authors.forename: Claudio
authors.initials: C
authors.lastname: Pannuti
authors.forename: Manuel
authors.initials: M
authors.lastname: de la Rosa
authors.forename: Elmer
authors.initials: E
authors.lastname: Salinas-Prieto
authors.forename: Gerardo
authors.initials: G
authors.lastname: Mendoza
mesh_terms: D000328:Adult; D000368:Aged; D055113:Chronic Periodontitis / Q000235:genetics*; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010568:Peru; D011110:Polymorphism, Genetic; D055815:Young Adult
chemical_list: D016753:Interleukin-10