title: Prognostic evaluation of DNA index in HIV-HPV co-infected women cervical samples attending in reference centers for HIV-AIDS in Recife.
abstract: BACKGROUND
  Persistence of cervical infection caused by human papillomavirus (HPV) types with high oncogenic risk may lead to cervical intraepithelial neoplasia (CIN). The aim of the present study was to evaluate whether, in HIV-positive women, the presence of aneuploidy in cervical cell samples is associated with presence and evolution of CIN.
  
  
  METHODS
  The present study had two stages. In the first stage, comprising a cross-sectional study, the association between the presence of aneuploidy seen via flow cytometry and sociodemographic characteristics, habits and characteristics relating to HPV and HIV infection was analyzed. In the second stage, comprising a cohort study, it was investigated whether aneuploidy was predictive of CIN evolution.
  
  
  RESULTS
  No association was observed between the presence of aneuploidy and HPV infection, or between its presence and alterations seen in oncotic cytological analysis. On the other hand, aneuploidy was associated with the presence of CIN (p = 0.030) in histological analysis and with nonuse of antiretroviral therapy (p = 0.001). Most of the HIV-positive women (234/272) presented normal CD4+ T lymphocyte counts (greater than 350 cells/mm3) and showed a greater aneuploidy regression rate (77.5%) than a progression rate (23.9%) over a follow-up of up to two years.
  
  
  CONCLUSIONS
  Although there was an association between the presence of cervical tissue lesions and the DNA index, the latter was not predictive of progression of the cervical lesion. This suggests that progression of the cervical lesion to cancer in HIV-positive women may also be changed through improvement of the immunological state enabled by using antiretroviral therapy.
authors.affiliation: Laboratório de imunogenética, Centro de pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, Brasil; Laboratório de biologia molecular, Departamento de oncologia pediátrica, Hospital IMIP, Recife, Brasil.
authors.forename: Albert Eduardo Silva
authors.initials: AE
authors.lastname: Martins
authors.affiliation: Laboratório de imunogenética, Centro de pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, Brasil; Laboratório de biologia molecular, Departamento de oncologia pediátrica, Hospital IMIP, Recife, Brasil.
authors.forename: Norma
authors.initials: N
authors.lastname: Lucena-Silva
authors.affiliation: Laboratório de imunogenética, Centro de pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, Brasil.
authors.forename: Renan Gomes
authors.initials: RG
authors.lastname: Garcia
authors.affiliation: Departamento Materno-Infantil e Centro Integrado de Saúde Amaury de Medeiro (CISAM-UPE), Universidade de Pernambuco, Recife, Brasil.
authors.forename: Stefan
authors.initials: S
authors.lastname: Welkovic
authors.affiliation: Departamento Materno-Infantil e Centro Integrado de Saúde Amaury de Medeiro (CISAM-UPE), Universidade de Pernambuco, Recife, Brasil.
authors.forename: Aureliana
authors.initials: A
authors.lastname: Barbosa
authors.affiliation: Departamento Materno-Infantil e Centro Integrado de Saúde Amaury de Medeiro (CISAM-UPE), Universidade de Pernambuco, Recife, Brasil.
authors.forename: Maria Luiza Bezerra
authors.initials: ML
authors.lastname: Menezes
authors.affiliation: Departamento Materno-Infantil, Universidade Federal de Pernambuco, Recife, Brasil.
authors.forename: Terezinha
authors.initials: T
authors.lastname: Tenório
authors.affiliation: Hospital Correia Picanço, Secretaria de Saúde de Pernambuco, Recife, Brasil.
authors.forename: Magda
authors.initials: M
authors.lastname: Maruza
authors.affiliation: Departamento de Medicina Tropical, Universidade Federal de Pernambuco (UFPE), Recife, Brasil.
authors.forename: Ricardo A A
authors.initials: RA
authors.lastname: Ximenes
mesh_terms: D000368:Aged; D002584:Cervix Uteri / Q000473:pathology / Q000821:virology*; D003430:Cross-Sectional Studies; D004247:DNA / Q000235:genetics; D005260:Female; D015658:HIV Infections / Q000235:genetics / Q000473:pathology*; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000235:genetics* / Q000473:pathology*; D011003:Ploidies; D011379:Prognosis; D002578:Uterine Cervical Dysplasia / Q000473:pathology
chemical_list: D004247:DNA