title: The phosphoproteome of Aspergillus nidulans reveals functional association with cellular processes involved in morphology and secretion.
abstract: We describe the first phosphoproteome of the model filamentous fungus Aspergillus nidulans. Phosphopeptides were enriched using titanium dioxide, separated using a convenient ultra-long reverse phase gradient, and identified using a "high-high" strategy (high mass accuracy on the parent and fragment ions) with higher-energy collisional dissociation. Using this approach 1801 phosphosites, from 1637 unique phosphopeptides, were identified. Functional classification revealed phosphoproteins were overrepresented under GO categories related to fungal morphogenesis: "sites of polar growth," "vesicle mediated transport," and "cytoskeleton organization." In these same GO categories, kinase-substrate analysis of phosphoproteins revealed the majority were target substrates of CDK and CK2 kinase families, indicating these kinase families play a prominent role in fungal morphogenesis. Kinase-substrate analysis also identified 57 substrates for kinases known to regulate secretion of hydrolytic enzymes (e.g. PkaA, SchA, and An-Snf1). Altogether this data will serve as a benchmark that can be used to elucidate regulatory networks functionally associated with fungal morphogenesis and secretion. All MS data have been deposited in the ProteomeXchange with identifier PXD000715 (http://proteomecentral.proteomexchange.org/dataset/PXD000715).
authors.affiliation: Department of Chemical, Biochemical and Environmental Engineering, UMBC, Baltimore, MD, USA.
authors.forename: Nikhil
authors.initials: N
authors.lastname: Ramsubramaniam
authors.forename: Steven D
authors.initials: SD
authors.lastname: Harris
authors.forename: Mark R
authors.initials: MR
authors.lastname: Marten
mesh_terms: D001233:Aspergillus nidulans / Q000166:cytology* / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D025941:Protein Interaction Mapping; D020543:Proteome / Q000378:metabolism; D040901:Proteomics; D049490:Systems Biology
chemical_list: D005656:Fungal Proteins; D010750:Phosphoproteins; D020543:Proteome
keywords: Aspergillus nidulans; Morphology; Phosphoproteomic analysis; Secretion; Systems biology