title: Gene profiling analysis for patients with oral verrucous carcinoma and oral squamous cell carcinoma.
abstract: Oral verrucous carcinoma (OVC) is one malignant tumor which was carved out from the oral squamous cell carcinoma (OSCC). However, the clinical and pathological features as well as the treatment strategies of OVC are different from OSCC. Here, global transcript abundance of tumor tissues from five patients with primary OVC and six patients with primary OSCC including their matched adjacently normal oral mucosa were profiled using the Affymetrix HGU133 Plus 2.0. Ingenuity Systems IPA software was used to analyse the gene function and biological pathways. There were 109 differentially expressed genes (more than 2-fold) between OVC and the adjacently normal tissue, among them 66 were up-regulated and 43 were down-regulated; 1172 differentially expressed genes (2-fold) between OSCC and the adjacently normal tissue, among them 608 were up-regulated and 564 were down-regulated. There were 39 common differentially expressed genes in OVC and OSCC compared with their matched normal oral mucosa, among them 22 up-regulated and 17 down-regulated, and 8 of them different between OVC and OSCC. In addition, the gene expression profile was further validated by quantitative real-time PCR (Q-RT-PCR) analysis for four of those 39 selected genes.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Xiangya Hospital and School of Stomatology, Central South University Changsha 410078, China.
authors.forename: Yue-Hong
authors.initials: YH
authors.lastname: Wang
authors.affiliation: Section of Nephrology, School of Medicine, Yale University 1 Gilbert Street, TAC #S360, New Haven, CT, 06510, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Tian
authors.affiliation: Department of Oral and Maxillofacial Surgery, Xiangya Hospital and School of Stomatology, Central South University Changsha 410078, China.
authors.forename: Ou-Sheng
authors.initials: OS
authors.lastname: Liu
authors.affiliation: Department of Oral and Maxillofacial Surgery, Xiangya Hospital and School of Stomatology, Central South University Changsha 410078, China.
authors.forename: Xiao-Dan
authors.initials: XD
authors.lastname: Fang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Xiangya Hospital and School of Stomatology, Central South University Changsha 410078, China.
authors.forename: Hong-Zhi
authors.initials: HZ
authors.lastname: Quan
authors.affiliation: Department of Oral and Maxillofacial Surgery, Xiangya Hospital and School of Stomatology, Central South University Changsha 410078, China.
authors.forename: Shang
authors.initials: S
authors.lastname: Xie
authors.affiliation: The Interdisciplinary Nanoscience Center (iNANO) and Department of Molecular Biology and Genetics, Aarhus University 8000 Aarhus C, Denmark ; Kunshan RNAi Institute Kunshan, Jiangsu 215300, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Oral and Maxillofacial Surgery, Xiangya Hospital and School of Stomatology, Central South University Changsha 410078, China.
authors.forename: Zhan-Gui
authors.initials: ZG
authors.lastname: Tang
keywords: Oral verrucous carcinoma; biological pathways; expression profile; oral squamous cell carcinoma; quantitative real-time PCR