title: Crystal structure of the homology domain of the eukaryotic DNA replication proteins Sld3/Treslin.
abstract: The initiation of eukaryotic chromosomal DNA replication requires the formation of an active replicative helicase at the replication origins of chromosomal DNA. Yeast Sld3 and its metazoan counterpart Treslin are the hub proteins mediating protein associations critical for the helicase formation. Here, we show the crystal structure of the central domain of Sld3 that is conserved in Sld3/Treslin family of proteins. The domain consists of two segments with 12 helices and is sufficient to bind to Cdc45, the essential helicase component. The structure model of the Sld3-Cdc45 complex, which is crucial for the formation of the active helicase, is proposed.
authors.affiliation: Structural Biology Center, National Institute of Genetics, Yata1111, Mishima, Shizuoka 411-8540, Japan; Department of Genetics, SOKENDAI, Yata1111, Mishima, Shizuoka 411-8540, Japan. Electronic address: hitou@nig.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Itou
authors.affiliation: Division of Microbial Genetics, National Institute of Genetics, Yata1111, Mishima, Shizuoka 411-8540, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Muramatsu
authors.affiliation: Structural Biology Center, National Institute of Genetics, Yata1111, Mishima, Shizuoka 411-8540, Japan; Department of Genetics, SOKENDAI, Yata1111, Mishima, Shizuoka 411-8540, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Shirakihara
authors.affiliation: Division of Microbial Genetics, National Institute of Genetics, Yata1111, Mishima, Shizuoka 411-8540, Japan; Department of Genetics, SOKENDAI, Yata1111, Mishima, Shizuoka 411-8540, Japan. Electronic address: hiaraki@nig.ac.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Araki
mesh_terms: D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000378:metabolism; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry; D054730:Protein Interaction Domains and Motifs; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000378:metabolism; D040681:Structural Homology, Protein
chemical_list: C102209:CDC45 protein, S cerevisiae; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; C426020:Sld3 protein, S cerevisiae