title: Ribozymes that can be regulated by external stimuli.
abstract: Ribozymes have been known for about 30 years, and nowadays are understood well enough to be turned into useful tools for a number of applications in vitro and in vivo. Allosteric ribozymes switch on and off their activity in response to a specific chemical (ligand) or physical (temperature, light) signal. The possibility of controlling ribozyme activity by external stimuli is of particular relevance for applications in different fields, such as environmental and medicinal diagnostics, molecular computing, control of gene expression and others. Herein, we review recent advances and describe selected examples of addressable ribozymes.
authors.affiliation: Ernst Moritz Arndt University Greifswald, Institute for Biochemistry, Felix Hausdorff Str. 4, D-17487 Greifswald, Germany.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Frommer
authors.affiliation: Ernst Moritz Arndt University Greifswald, Institute for Biochemistry, Felix Hausdorff Str. 4, D-17487 Greifswald, Germany.
authors.forename: Bettina
authors.initials: B
authors.lastname: Appel
authors.affiliation: Ernst Moritz Arndt University Greifswald, Institute for Biochemistry, Felix Hausdorff Str. 4, D-17487 Greifswald, Germany. Electronic address: smueller@uni-greifswald.de.
authors.forename: Sabine
authors.initials: S
authors.lastname: Müller
mesh_terms: D000494:Allosteric Regulation; D006801:Humans; D008024:Ligands; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism*; D013696:Temperature
chemical_list: D008024:Ligands; D009841:Oligonucleotides; D016337:RNA, Catalytic