title: Classical swine fever virus and p7 protein induce secretion of IL-1β in macrophages.
abstract: Classical swine fever virus (CSFV) has a tropism for vascular endothelial cells and immune system cells. The process and release of pro-inflammatory cytokines, including IL-1β and IL-18, is one of the fundamental reactions of the innate immune response to viral infection. In this study, we investigated the production of IL-1β from macrophages following CSFV infection. Our results showed that IL-1β was upregulated after CSFV infection through activating caspase-1. Subsequent studies demonstrated that reactive oxygen species may not be involved in CSFV-mediated IL-1β release. Recently, research has indicated a novel mechanism by which inflammasomes are triggered through detection of activity of viroporin. We further demonstrated that CSFV viroporin p7 protein induced IL-1β secretion which could be inhibited by the ion channel blocker amantadine and also discovered that p7 protein was a short-lived protein degraded by the proteasome. Together, our observations provided an insight into the mechanism of CSFV-induced inflammatory responses.
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling 712100, Shaanxi, PR China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Lin
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling 712100, Shaanxi, PR China.
authors.forename: Wulong
authors.initials: W
authors.lastname: Liang
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling 712100, Shaanxi, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Kang
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling 712100, Shaanxi, PR China.
authors.forename: Helin
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling 712100, Shaanxi, PR China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Cao
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling 712100, Shaanxi, PR China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D020170:Caspase 1 / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D006692:Classical Swine Fever Virus / Q000502:physiology*; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism / Q000821:virology*; D013552:Swine; D014764:Viral Proteins / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D053583:Interleukin-1beta; D014764:Viral Proteins; D020170:Caspase 1