title: Diversity of selected Lupinus angustifolius L. genotypes at the phenotypic and DNA level with respect to microscopic seed coat structure and thickness.
abstract: The paper investigates seed coat characteristics (as a percentage of overall seed diameter) in Lupinus angustifolius L., a potential forage crop. In the study ten L. angustifolius genotypes, including three Polish cultivars, two Australian cultivars, three mutants originated from cv. 'Emir', and one Belarusian and one Australian breeding line were evaluated. The highest seed coat percentage was recorded in cultivars 'Sonet' and 'Emir'. The lowest seed coat thickness percentage (below 20%) was noted for breeding lines 11257-19, LAG24 and cultivar 'Zeus' (17.87%, 18.91% 19.60%, respectively). Despite having low seed weight, the Australian line no. 11257-19 was characterized by a desirable proportion of seed coat to the weight of seeds. In general, estimation of the correlation coefficient indicated a tendency that larger seeds had thinner coats. Scanning Electron Microscopy images showed low variation of seed coat sculpture and the top of seeds covered with a cuticle. Most of the studied genotypes were characterized by a cristatepapillate seed coat surface, formed by elongated polygonal cells. Only breeding line no. 11267-19 had a different shape of the cells building the surface layer of the coat. In order to illustrate genetic diversity among the genotypes tested, 24 ISSR primers were used. They generated a total of 161 polymorphic amplification products in 10 evaluated narrow-leaved lupin genotypes.
authors.affiliation: Centre for Plant Genetics and Breeding, University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Jon
authors.initials: J
authors.lastname: Clements
authors.affiliation: Department of Genetics, Plant Breeding and Seed Production, Wroclaw University of Environmental and Life Sciences, Wroclaw, Lower Silesia, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Galek
authors.affiliation: Department of Genetics, Plant Breeding and Seed Production, Wroclaw University of Environmental and Life Sciences, Wroclaw, Lower Silesia, Poland.
authors.forename: Bartosz
authors.initials: B
authors.lastname: Kozak
authors.affiliation: Department of Plant Physiology and Biotechnology, University of Warmia and Mazury, Olsztyn, Warmian-Masurian, Poland.
authors.forename: Dariusz Jan
authors.initials: DJ
authors.lastname: Michalczyk
authors.affiliation: Department of Plant Physiology and Biotechnology, University of Warmia and Mazury, Olsztyn, Warmian-Masurian, Poland.
authors.forename: Agnieszka Iwona
authors.initials: AI
authors.lastname: Piotrowicz-Cieślak
authors.affiliation: Department of Genetics, Plant Breeding and Seed Production, Wroclaw University of Environmental and Life Sciences, Wroclaw, Lower Silesia, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Sawicka-Sienkiewicz
authors.affiliation: Plant Breeding Station, "HR Smolice Sp. z o. o. Grupa IHAR" Przebedowo, Greater Poland, Poland.
authors.forename: Stanislaw
authors.initials: S
authors.lastname: Stawiński
authors.affiliation: Department of Genetics, Plant Breeding and Seed Production, Wroclaw University of Environmental and Life Sciences, Wroclaw, Lower Silesia, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Zalewski
mesh_terms: D016000:Cluster Analysis; D056726:Genetic Association Studies; D014644:Genetic Variation; D005838:Genotype; D031297:Lupinus / Q000033:anatomy & histology / Q000235:genetics* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010641:Phenotype; D011110:Polymorphism, Genetic; D012639:Seeds / Q000033:anatomy & histology / Q000235:genetics* / Q000648:ultrastructure