title: Proteins immobilization on the surface of modified plant viral particles coated with hydrophobic polycations.
abstract: Two hydrophobic cations based on poly-N-ethyl-vinylpyridine were used to produce biologically active complexes. The complexes obtained from tobacco mosaic virus (TMV) spherical particles (SPs), hydrophobic polycation, and a model protein were stable and did not aggregate in solution, particularly at high ionic strengths. The nucleic acid-free SPs were generated by thermal remodeling of the TMV (helical rod-shaped plant virus). The model protein preserved its antigenic activity in the ternary complex (SP-polycation-protein). Immobilization of proteins on the surface of SPs coated with hydrophobic cation is a promising approach to designing biologically active complexes used in bionanotechnologies.
authors.affiliation: a Faculty of Biology , Lomonosov Moscow State University , Moscow , Russia.
authors.forename: Nikolai A
authors.initials: NA
authors.lastname: Nikitin
authors.forename: Andrei S
authors.initials: AS
authors.lastname: Malinin
authors.forename: Ekaterina A
authors.initials: EA
authors.lastname: Trifonova
authors.forename: Anna A
authors.initials: AA
authors.lastname: Rakhnyanskaya
authors.forename: Aleksandr A
authors.initials: AA
authors.lastname: Yaroslavov
authors.forename: Olga V
authors.initials: OV
authors.lastname: Karpova
authors.forename: Joseph G
authors.initials: JG
authors.lastname: Atabekov
mesh_terms: D000818:Animals; D002417:Cattle; D057927:Hydrophobic and Hydrophilic Interactions; D055767:Immobilized Proteins / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry; D037121:Plant Lectins; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes; D011145:Polyvinyls / Q000737:chemistry; D017863:Potexvirus; D055550:Protein Stability; D011726:Pyridinium Compounds / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012996:Solutions; D013057:Spectrum Analysis; D014027:Tobacco Mosaic Virus; D014771:Virion / Q000737:chemistry*
chemical_list: D055767:Immobilized Proteins; D007477:Ions; D037121:Plant Lectins; D011073:Polyamines; D000071228:Polyelectrolytes; D011145:Polyvinyls; D011726:Pyridinium Compounds; D012710:Serum Albumin, Bovine; D012996:Solutions; C041384:Datura stramonium lectin; C086116:poly(N-ethyl-4-vinylpyridine bromide)
keywords: biologically active complexes; hydrophobic polycations; spherical particles; tobacco mosaic virus