title: Combinatorial gene therapy accelerates bone regeneration: non-viral dual delivery of VEGF and BMP2 in a collagen-nanohydroxyapatite scaffold.
abstract: Vascularization and bone repair are accelerated by a series of gene-activated scaffolds delivering both an angiogenic and an osteogenic gene. Stem cell-mediated osteogenesis in vitro, in addition to increased vascularization and bone repair by host cells in vivo, is enhanced using all systems while the use of the nanohydroxyapatite vector to deliver both genes markedly enhances bone healing.
authors.affiliation: Tissue Engineering Research Group, Department of Anatomy, Royal College of Surgeons in Ireland, Dublin, Ireland; Trinity Centre for Bioengineering, Trinity College Dublin, Dublin, Ireland; Advanced Materials and BioEngineering Research (AMBER) Centre Royal College of Surgeons in Ireland & Trinity College Dublin, Dublin, Ireland.
authors.forename: Caroline M
authors.initials: CM
authors.lastname: Curtin
authors.forename: Erica G
authors.initials: EG
authors.lastname: Tierney
authors.forename: Kevin
authors.initials: K
authors.lastname: McSorley
authors.forename: Sally-Ann
authors.initials: SA
authors.lastname: Cryan
authors.forename: Garry P
authors.initials: GP
authors.lastname: Duffy
authors.forename: Fergal J
authors.initials: FJ
authors.lastname: O'Brien
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D003094:Collagen / Q000494:pharmacology*; D016503:Drug Delivery Systems; D017886:Durapatite / Q000494:pharmacology*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D051379:Mice; D018919:Neovascularization, Physiologic / Q000187:drug effects; D011094:Polyethyleneimine / Q000494:pharmacology; D051381:Rats; D054457:Tissue Scaffolds / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D055396:Bone Morphogenetic Protein 2; D042461:Vascular Endothelial Growth Factor A; C509317:nano-hydroxyapatite-collagen; D011094:Polyethyleneimine; D003094:Collagen; D017886:Durapatite
keywords: bone regeneration; collagen scaffolds; gene-activated scaffolds; mesenchymal stem cells; non-viral gene therapy