title: Modeling of rhythmic behavior in neutral lipid production due to continuous supply of limited nitrogen: mutual growth and lipid accumulation in microalgae.
abstract: The relative effects of three precise nitrogen limitation regimes on green micro-algae were assessed using the Trebouxiophycean alga Coccomyxa subellipsoidea grown in a chemostatic bioreactor system. The data provides further evidence that growth and triglyceride (TAG) accumulation are concurrent and independently proportional to the degree of nitrogen limitation in algae. Additionally, TAG accumulation was observed to proceed via oscillations with respect to time and percent dry weight quantity. The predator-prey model was applied to fit the experimental data and to obtain the physiological significance of these oscillations. The results determine the conditions of maximum neutral lipid productivity with respect to nitrate stress and highlight an area of potential future research.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA; School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588, USA. Electronic address: rahultevatia_83@yahoo.co.in.
authors.forename: Rahul
authors.initials: R
authors.lastname: Tevatia
authors.affiliation: Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
authors.forename: James
authors.initials: J
authors.lastname: Allen
authors.affiliation: School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Blum
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
authors.forename: Yaşar
authors.initials: Y
authors.lastname: Demirel
authors.affiliation: Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Black
mesh_terms: D018533:Biomass; D019149:Bioreactors; D000460:Chlorophyta / Q000254:growth & development / Q000378:metabolism*; D002853:Chromatography, Liquid; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism*; D008954:Models, Biological; D009584:Nitrogen / Q000172:deficiency*; D010507:Periodicity; D053719:Tandem Mass Spectrometry; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D014280:Triglycerides; D009584:Nitrogen
keywords: Continuous nitrogen limitation; Maximum productivities; Microalgal growth; Neutral lipid accumulation; Prey–predator model