title: [The relevance of the trace elements zinc and iron in the milk fever disease of cattle].
abstract: OBJECTIVE
  The aim of this study was to analyse the concentrations of Zn and Fe as well as their relationships to metabolic parameters in milk fever cows.
  
  
  METHODS
  A total of 195 Simmental cows, downer cows and clinically healthy control animals were divided into five groups: a) control group (CG, n = 21), b) all cows with milk fever (MF) (n = 174), c) MF cows without additional diseases (n = 145), d) cows with MF and mastitis (n = 10) and e) cows with retained placenta or endometritis (n = 19). Selenium (Se), zinc (Zn), iron (Fe), calcium (Ca), inorganic phosphorus (Pi), tumour necrosis factor α (TNFα), haptoglobin (Hp), antioxidants (Trolox Equivalent Antioxidative Capacity: TEAC), non-esterified fatty acids (NEFA), beta-hydroxybutyrate (BHB), bilirubin, urea, creatinine, glucose, cholesterol, gamma-glutamyl transferase (GGT) and alkaline phosphatase (AP) were analysed in the blood serum.
  
  
  RESULTS
  The concentrations of Zn, Fe, Ca, Pi and TEAC were lower in groups b) to e) whereas Hp was higher than in the CG (p ≤ 0.05). In group c), lower Ca and Pi concentrations were found when compared to groups d) and e) (p ≤ 0.05). In group e), Zn concentrations were significantly lower than in group c) (p ≤ 0.05). Zn was negatively correlated with K (CG) and positively correlated with TEAC, Cu, Mn and Fe (groups b and c) and with Mn (group e) (p ≤ 0.05). Fe was positively correlated with Ca (group c), Pi (group c), K (groups b and c) and Mg (groups b-d) as well as with Zn, Cu and Se (groups b and c) (p ≤ 0.05). In groups b) and c), TNFα was increased and negatively correlated with Fe (p ≤ 0.05). AP activity in groups b) and e) was lower than in the CG (p ≤ 0.05).
  
  
  CONCLUSIONS
  These results and literature data support the hypothesis that Zn and Fe could be engaged in bone metabolism and be involved in the pathogenesis of MF. The concentrations of Hp and TEAC support this interpretation. Control of the Zn and Fe status of cows and Zn supplementation should be included in the prevention and advanced therapy of MF.
authors.affiliation: Mandy Heilig, Dürrenberger Straße 12, 04177 Leipzig, E-Mail: mobinet@gmx.de.
authors.forename: M
authors.initials: M
authors.lastname: Heilig
authors.forename: D
authors.initials: D
authors.lastname: Bäuml
authors.forename: M
authors.initials: M
authors.lastname: Fürll
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood*; D003612:Dairying; D005260:Female; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007501:Iron / Q000097:blood*; D010319:Parturient Paresis / Q000097:blood*; D011247:Pregnancy; D014131:Trace Elements / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D015032:Zinc / Q000097:blood*
chemical_list: D014131:Trace Elements; D014409:Tumor Necrosis Factor-alpha; D007334:Insulin-Like Growth Factor I; D007501:Iron; D015032:Zinc
keywords: Dairy cow; IGF-1; TNFα; bone; calcium; downer cow; metabolic parameters; parturient paresis; trace elements