title: Over-expression of Oct4 and Sox2 transcription factors enhances differentiation of human umbilical cord blood cells in vivo.
abstract: Gene and cell-based therapies comprise innovative aspects of regenerative medicine. Even though stem cells represent a highly potential therapeutic strategy, their wide-spread exploitation is marred by ethical concerns, potential for malignant transformation and a plethora of other technical issues, largely restricting their use to experimental studies. Utilizing genetically modified human umbilical cord blood mono-nuclear cells (hUCB-MCs), this communication reports enhanced differentiation of transplants in a mouse model of amyotrophic lateral sclerosis (ALS). Over-expressing Oct4 and Sox2 induced production of neural marker PGP9.5, as well as transformation of hUCB-MCs into micro-glial and endothelial lines in ALS spinal cords. In addition to producing new nerve cells, providing degenerated areas with trophic factors and neo-vascularisation might prevent and even reverse progressive loss of moto-neurons and skeletal muscle paralysis.
authors.affiliation: Kazan State Medical University, Kazan, Republic of Tatarstan, Russian Federation; Hannover Medical School, Hannover, Germany.
authors.forename: Daria
authors.initials: D
authors.lastname: Guseva
authors.affiliation: Kazan Federal University, Kazan, Republic of Tatarstan, Russian Federation.
authors.forename: Albert A
authors.initials: AA
authors.lastname: Rizvanov
authors.affiliation: Kazan Federal University, Kazan, Republic of Tatarstan, Russian Federation.
authors.forename: Ilnur I
authors.initials: II
authors.lastname: Salafutdinov
authors.affiliation: Kazan Federal University, Kazan, Republic of Tatarstan, Russian Federation.
authors.forename: Nezhdana V
authors.initials: NV
authors.lastname: Kudryashova
authors.affiliation: Kazan Federal University, Kazan, Republic of Tatarstan, Russian Federation; Asklepios-Med (Private Medical Practice and Research Center), Szeged, Hungary. Electronic address: palotas@asklepios-med.eu.
authors.forename: András
authors.initials: A
authors.lastname: Palotás
authors.affiliation: Kazan State Medical University, Kazan, Republic of Tatarstan, Russian Federation. Electronic address: islamru@yahoo.com.
authors.forename: Rustem R
authors.initials: RR
authors.lastname: Islamov
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics; D000818:Animals; D054337:Cell Dedifferentiation; D002454:Cell Differentiation; D004195:Disease Models, Animal; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis*; D039904:Pluripotent Stem Cells / Q000378:metabolism; D044968:Regenerative Medicine; D055748:SOXB1 Transcription Factors / Q000096:biosynthesis*; D014162:Transfection
chemical_list: D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors
keywords: Amyotrophic lateral sclerosis; Differentiation; Pluripotency; Transcription factor; Umbilical cord blood mono-nuclear cell