title: Transdermal delivery of 10,11-methylenedioxycamptothecin by hyaluronic acid based nanoemulsion for inhibition of keloid fibroblast.
abstract: This study designs an alternative transdermal delivery system for 10,11-methylenedioxycamptothecin(MD-CPT) to inhibit keloid. Hyaluronic acid nanoemulsions (HANs) with nano size, negative charge and good stability were prepared as transdermal carriers. The MD-CPT loaded HANs performed desirable skin permeable capacity across human keloid skin and the drug was transferred directly to keloid lesion area. MD-CPT was delivered percutaneously higher than the control group. FITC-HANs could be successfully internalized by keloid fibroblast (KF) and deliver MD-CPT toward nucleus, inhibited the proliferation of KF, while there was no serious toxicity to normal skin fibroblasts. The growth-inhibitory effect was further clarified upon cell cycle regulation, which arrested cells at G1/S and prevented them entry into mitosis. KF gene expression demonstrated plasminogen activator inhibitor-1 (PAI-1) was significantly down-regulated and Smad7 up-regulated, which was beneficial to inhibit keloid. The study demonstrated that as transdermal delivery of MD-CPT by HANs has potential for inhibition of keloid fibroblast.
authors.affiliation: College of Marine Life Science, Ocean University of China, Yushan Road, Qingdao 266003, Shandong, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: College of Marine Life Science, Ocean University of China, Yushan Road, Qingdao 266003, Shandong, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Department of Plastic Surgery, The Affiliated Hospital of Medical College Qingdao University, Qingdao 266013, Shandong, China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Marine Life Science, Ocean University of China, Yushan Road, Qingdao 266003, Shandong, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Marine Life Science, Ocean University of China, Yushan Road, Qingdao 266003, Shandong, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Gao
authors.affiliation: Department of Plastic Surgery, The Affiliated Hospital of Medical College Qingdao University, Qingdao 266013, Shandong, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Marine Life Science, Ocean University of China, Yushan Road, Qingdao 266003, Shandong, China. Electronic address: kongming@ouc.edu.cn.
authors.forename: Ming
authors.initials: M
authors.lastname: Kong
authors.affiliation: College of Marine Life Science, Ocean University of China, Yushan Road, Qingdao 266003, Shandong, China. Electronic address: xgchen@ouc.edu.cn.
authors.forename: Xiguang
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D002166:Camptothecin / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D004655:Emulsions / Q000008:administration & dosage / Q000737:chemistry; D005347:Fibroblasts / Q000187:drug effects* / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D007627:Keloid / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D008938:Mitosis / Q000187:drug effects; D049329:Nanostructures / Q000008:administration & dosage / Q000737:chemistry; D017208:Rats, Wistar; D012867:Skin / Q000166:cytology / Q000187:drug effects; D014018:Tissue Distribution
chemical_list: C120496:10,11-methylenedioxycamptothecin; D004655:Emulsions; D006820:Hyaluronic Acid; D002166:Camptothecin
keywords: 10,11-Methylenedioxycamptothecin; Hyaluronic acid; Keloid fibroblast; Nanoemulsion; Transdermal delivery