title: Neuropeptide AF induces anxiety-like and antidepressant-like behavior in mice.
abstract: Little is known about the action of neuropeptide AF (NPAF) on anxiety and depression. Only our previous study provides evidence that NPAF induces anxiety-like behavior in rats. Therefore, the aim of the present study was to investigate the action of NPAF on depression-like behavior and the underlying neurotransmissions in mice. In order to determine whether there are species differences between rats and mice, we have investigated the action of NPAF on anxiety-like behavior in mice as well. A modified forced swimming test (mFST) and an elevated plus maze test (EPMT) were used to investigate the depression and anxiety-related behaviors, respectively. Mice were treated with NPAF 30min prior to the tests. In the mFST, the animals were pretreated with a non-selective muscarinic acetylcholine receptor antagonist, atropine, a non-selective 5-HT2 serotonergic receptor antagonist, cyproheptadine, a mixed 5-HT1/5-HT2 serotonergic receptor antagonist, methysergide, a D2/D3/D4 dopamine receptor antagonist, haloperidol, a α1/α2β-adrenergic receptor antagonist, prazosin or a non-selective β-adrenergic receptor antagonist, propranolol 30min before the NPAF administration. In the mFST, NPAF decreased the immobility time and increased the climbing and swimming times. This action was reversed completely by methysergide and partially by atropine, whereas cyproheptadine, haloperidol, prazosin and propranolol were ineffective. In the EPMT, NPAF decreased the time spent in the arms (open/open+closed). Our results demonstrate that NPAF induces anti-depressant-like behavior in mice, which is mediated, at least in part, through 5HT2-serotonergic and muscarinic cholinergic neurotransmissions. In addition, the NPAF-induced anxiety is species-independent, since it develops also in mice.
authors.affiliation: Department of Pathophysiology, Faculty of Medicine, University of Szeged, Hungary.
authors.forename: Miklós
authors.initials: M
authors.lastname: Palotai
authors.affiliation: Department of Pathophysiology, Faculty of Medicine, University of Szeged, Hungary; MTA-SZTE Neuroscience Research Group of the Hungarian Academy of Sciences, Szeged, Hungary. Electronic address: telegdy.gyula@med.u-szeged.hu.
authors.forename: Gyula
authors.initials: G
authors.lastname: Telegdy
authors.affiliation: MTA-SZTE Neuroscience Research Group of the Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: Masaru
authors.initials: M
authors.lastname: Tanaka
authors.affiliation: Department of Pathophysiology, Faculty of Medicine, University of Szeged, Hungary.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Bagosi
authors.affiliation: Department of Pathophysiology, Faculty of Medicine, University of Szeged, Hungary.
authors.forename: Miklós
authors.initials: M
authors.lastname: Jászberényi
mesh_terms: D058668:Adrenergic alpha-1 Receptor Antagonists / Q000494:pharmacology; D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000818:Animals; D000928:Antidepressive Agents / Q000627:therapeutic use* / Q000633:toxicity*; D001007:Anxiety / Q000139:chemically induced*; D004195:Disease Models, Animal; D018492:Dopamine Antagonists / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006220:Haloperidol / Q000494:pharmacology; D046353:Immobility Response, Tonic / Q000187:drug effects; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D009842:Oligopeptides / Q000627:therapeutic use* / Q000633:toxicity*; D010643:Phenoxybenzamine / Q000494:pharmacology; D011224:Prazosin / Q000494:pharmacology; D018490:Serotonin Agents / Q000494:pharmacology; D013550:Swimming / Q000523:psychology
chemical_list: D058668:Adrenergic alpha-1 Receptor Antagonists; D000317:Adrenergic alpha-Antagonists; D000928:Antidepressive Agents; D018492:Dopamine Antagonists; D009842:Oligopeptides; D018490:Serotonin Agents; D010643:Phenoxybenzamine; C061209:A18Famide; D006220:Haloperidol; D011224:Prazosin
keywords: Elevated plus maze test; Forced swimming test; Neuropeptide AF; Neurotransmitter