title: HMGB1 enhances smooth muscle cell proliferation and migration in pulmonary artery remodeling.
abstract: HMGB1 is a necessary and critical mediator of acute lung injury and can act as a chemoattractant and anti-apoptosis factor in injury or repair in diseases. In this study we sought to determine whether HMGB1 is involved in the remodeling of pulmonary artery and investigate the mechanism. A rat model of pulmonary artery remodeling was successful induced with LPS infusion and the increasing of pulmonary arteries media was obviously inhibited in rats treated with thrice inject of HMGB1 neutralizing antibody. The percent of areas of tunica media to total artery wall was (0.53 ± 0.15), (0.81 ± 0.10) and (0.59 ± 0.11) in control, LPS and antibody group respectively (p<0.05). Meanwhile, treatment with HMGB1 neutralizing antibody not only decreased the level of HMGB1 mRNA and protein significantly, but inhibited the expression of PCAN and Bcl-2 as well. On the contrary, Bax, a gen which represented the apoptosis, revealed an absolutely reversed trend to Bcl-2 in pulmonary arteries. Experiments in vitro showed that HMGB1 could stimulate the proliferation of hPASMC in MTT test and increase the number of migrated cells in a concentration-dependent manner in chemotaxis assay using modified Boyden chambers. In conclusion, data from this study support the concept that HMGB1 is involved in the remodeling of pulmonary artery by enhancing proliferation and migration of smooth muscle cell. Inhibiting HMGB1 may be a new target to deal with the remodeling of pulmonary artery.
authors.affiliation: Department of Anesthesiology, Qilu Hospital of Shandong University Jinan, Shandong, P.R. China.
authors.forename: Huan-Liang
authors.initials: HL
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Qilu Hospital of Shandong University Jinan, Shandong, P.R. China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Peng
authors.affiliation: Department of Anesthesiology, Qilu Hospital of Shandong University Jinan, Shandong, P.R. China.
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, Qilu Hospital of Shandong University Jinan, Shandong, P.R. China.
authors.forename: Shu-Hai
authors.initials: SH
authors.lastname: Tang
authors.affiliation: Department of Anesthesiology, Qilu Hospital of Shandong University Jinan, Shandong, P.R. China.
authors.forename: Bao-Zhu
authors.initials: BZ
authors.lastname: Sun
authors.affiliation: Department of Anesthesiology, Qilu Hospital of Shandong University Jinan, Shandong, P.R. China.
authors.forename: Chun-Ling
authors.initials: CL
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Qilu Hospital of Shandong University Jinan, Shandong, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Huang
authors.affiliation: Department of Anesthesiology, Qilu Hospital of Shandong University Jinan, Shandong, P.R. China.
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Xu
authors.affiliation: Department of Anesthesiology, Qilu Hospital of Shandong University Jinan, Shandong, P.R. China.
authors.forename: Wei-Fu
authors.initials: WF
authors.lastname: Lei
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D004195:Disease Models, Animal; D024243:HMGB1 Protein / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000473:pathology; D032389:Myocytes, Smooth Muscle / Q000378:metabolism / Q000473:pathology*; D011651:Pulmonary Artery / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D066253:Vascular Remodeling / Q000502:physiology*
chemical_list: D024243:HMGB1 Protein
keywords: HMGB1; apoptosis; migration; proliferation; pulmonary artery remodeling