title: A mitochondria-targetable fluorescent probe for dual-channel NO imaging assisted by intracellular cysteine and glutathione.
abstract: A mitochondria-specific fluorescent probe for NO (1) was synthesized by the direct conjugation of a pyronin dye with one of the amino groups of o-phenylenediamino (OPD). The probe could selectively detect NO over dehydroascorbic acid (DHA), ascorbic acid (AA), and methylglyoxal (MGO) as well as the reactive oxygen/nitrogen species (ROS/RNS) with the significant off-on response due to the production of a red-emission triazole 2. In the presence of cysteine/glutathione (Cys/GSH), 2 could be further transformed into a green-emission aminopyronin 4 and a red-emission thiopyronin 5, respectively. Assisted by intracellular Cys and GSH, the probe demonstrated its potential to monitor mitochondrial NO in a dual-channel mode.
authors.affiliation: School of Chemistry and Chemical Engineering and ‡Institute of Biotechnology, Shanxi University , Taiyuan 030006, China.
authors.forename: Yuan-Qiang
authors.initials: YQ
authors.lastname: Sun
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Hongxing
authors.initials: H
authors.lastname: Zhang
authors.forename: Yingying
authors.initials: Y
authors.lastname: Huo
authors.forename: Xin
authors.initials: X
authors.lastname: Lv
authors.forename: Yawei
authors.initials: Y
authors.lastname: Shi
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000378:metabolism*; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D009569:Nitric Oxide / Q000032:analysis* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D009569:Nitric Oxide; D005978:Glutathione; D003545:Cysteine