title: Mast cell phenotype in benign and malignant tumors of the prostate.
abstract: The molecular phenotypic heterogeneity of mast cells (MCs) makes them attractive as potential therapeutic targets in anti-cancer adjuvant therapy. Mast cell aggregations observed in tumors suggested their involvement in tumor pathogenesis. Despite several studies using mast cell tryptase, MCs' involvement in the progression of prostate tumors has not been demonstrated. The aim of our study was to identify and quantify the phenotypic heterogeneity of MCs in prostate lesions. Our study included 7 cases of normal prostate, 25 cases of benign epithelial hyperplasia and 64 cases of prostate carcinoma. MCs were immunohistochemically assessed using three markers: tryptase, chymase and CD117. Two immunophenotypes of MCs were identified in benign lesions: tryptase+/CD117+/chymase- and tryptase-/chymase+/CD117+, located in peritumoral areas. Intratumoral MC phenotype of malignant lesions was characterized by tryptase+/chymase+/CD117+, while in the peritumoral areas three different MCs phenotypes were identified: tryptase+/chymase+/CD117-, tryptase+/CD117+/chymase- and chymase+/CD117+/tryptase-. Our results suggest the correlation of chymase positive MCs of the peritumoral areas and CD117 positive MCs of the intratumoral areas with tumor grade.
authors.affiliation: Anca Maria Cimpean MD, PhD, Professor of Histology Department of Microscopic Morphology-Histology Angiogenesis Research Center "Victor Babes" University of Medicine and Pharmacy, Timisoara Piata Eftimie Murgu 2 300041, Timisoara, Romania tel. 0040720060955 e-mail: ancacimpean1972@yahoo.com.
authors.forename: T
authors.initials: T
authors.lastname: Globa
authors.forename: L
authors.initials: L
authors.lastname: Saptefrţi
authors.forename: R A
authors.initials: RA
authors.lastname: Ceauşu
authors.forename: P
authors.initials: P
authors.lastname: Gaje
authors.forename: A M
authors.initials: AM
authors.lastname: Cimpean
authors.forename: M
authors.initials: M
authors.lastname: Raica
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D001706:Biopsy; D053818:Chymases / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008407:Mast Cells / Q000201:enzymology* / Q000276:immunology* / Q000473:pathology; D060787:Neoplasm Grading; D010641:Phenotype; D011237:Predictive Value of Tests; D011470:Prostatic Hyperplasia / Q000201:enzymology* / Q000276:immunology* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000201:enzymology* / Q000276:immunology* / Q000473:pathology; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis; D053802:Tryptases / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D019009:Proto-Oncogene Proteins c-kit; D053818:Chymases; D053802:Tryptases