title: The CC chemokine ligand 2 (CCL2) polymorphism -2518A/G is associated with gout in the Chinese Han male population.
abstract: Gout is usually characterized by uric acid-induced recurrent attacks of acute inflammatory arthritis. CC chemokine ligand 2 (CCL2), a chemokine involved in the recruitment and migration of monocytes/macrophages, has previously been shown to be increased in the plasma of gout patients. In this study, we examined whether the CCL2 -2518A/G (rs1024611) single nucleotide polymorphism (SNP) affects susceptibility to gout in a Chinese Han male population. Genomic DNA from gout patients (n = 1,109) and ethnically matched gout-free controls (n = 1,034) was genotyped for the CCL2 -2518A/G SNP using polymerase chain reaction-restriction fragment length polymorphism. The Chi-square test was performed to investigate the association of genotypic and allelic frequencies between cases and controls, and the -2518G allele was shown to be associated with a significantly increased risk of gout development [P = 0.007, odds ratio 1.182, 95% confidence interval 1.047-1.335]. The GG genotypic distribution was also significantly different between cases and controls (adjusted P = 0.021). However, genotypic distributions and allelic frequencies did not indicate significant associations (P = 0.150 and P = 0.050, respectively) between tophi and non-tophi patients. Our findings support a key role for the CCL2 SNP -2518A/G in association with gout susceptibility in the Chinese Han male population. However, additional studies in other populations should be carried out to confirm this finding.
authors.affiliation: Shandong Provincial Key Laboratory of Metabolic Disease, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, 266003, China.
authors.forename: Ruixia
authors.initials: R
authors.lastname: Sun
authors.forename: Keke
authors.initials: K
authors.lastname: Zhang
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Zhang
authors.forename: Lingling
authors.initials: L
authors.lastname: Cui
authors.forename: Can
authors.initials: C
authors.lastname: Wang
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Mi
authors.forename: Shiguo
authors.initials: S
authors.lastname: Liu
authors.forename: Changgui
authors.initials: C
authors.lastname: Li
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000235:genetics*; D002681:China / Q000208:ethnology; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006073:Gout / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2