title: Decreased RGS6 expression is associated with poor prognosis in pancreatic cancer patients.
abstract: Regulator of G-protein signaling 6 (RGS6), a member of a family of RGS proteins, has been reported to involve in multiple processes during tumor development. However, its role in pancreatic cancer has not been studied yet. In this study, we aimed to investigate the expression of RGS6 in pancreatic cancer and its role in predicting outcomes of patients with pancreatic cancer. We first measured the expression of RGS6 mRNA in 20 cases of tumor tissues and matched adjacent non-tumorous tissues by quantitative real-time PCR and examined RGS6 protein by immunohistochemistry in tissue microarrays containing 90 tumor and 90 paired adjacent non-tumor tissues. Decreased RGS6 mRNA detected in primary tumor, compared with their non-tumor counterparts. In addition, decreased RGS6 protein expression was associated with tumor differentiation (P = 0.027), pT classification (P = 0.034), smoking status (P = 0.041) and a poor survival (P = 0.007). Cox proportional hazards regression modeling analysis revealed that lymph node metastasis (P = 0.001; hazard ratio, 2.347, 95% CI, 1.387-3.972), tumor differentiation (P = 0.015; hazard ratio, 0.505, 95% CI, 0.291-0.876) and RGS6 expression (P = 0.048; hazard ratio, 0.567, 95% CI, 0.324-0.994) were three independent prognostic factors. Taken together, these date demonstrate that RGS6 decreases in tumor tissue and may serve as a novel biomarker for outcomes in pancreatic cancer patients and be a potential therapeutic target potential therapeutic target.
authors.affiliation: Department of Surgical Oncology, Chinese People's Liberation Army (PLA) General Hospital 28 Fuxing Road, Beijing 100853, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Jiang
authors.affiliation: Department of Surgical Oncology, Chinese People's Liberation Army (PLA) General Hospital 28 Fuxing Road, Beijing 100853, China.
authors.forename: Ruihua
authors.initials: R
authors.lastname: Xue
authors.affiliation: Department of Cancer Center, Chinese People's Liberation Army (PLA) General Hospital 28 Fuxing Road, Beijing 100853, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Bu
authors.affiliation: Department of Cancer Center, Chinese People's Liberation Army (PLA) General Hospital 28 Fuxing Road, Beijing 100853, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Tong
authors.affiliation: Department of Cancer Center, Chinese People's Liberation Army (PLA) General Hospital 28 Fuxing Road, Beijing 100853, China.
authors.forename: Jiankun
authors.initials: J
authors.lastname: Qiang
authors.affiliation: Department of Surgical Oncology, Chinese People's Liberation Army (PLA) General Hospital 28 Fuxing Road, Beijing 100853, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D011379:Prognosis; D016016:Proportional Hazards Models; D020710:RGS Proteins / Q000032:analysis / Q000096:biosynthesis*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; D020710:RGS Proteins; C400194:RGS6 protein, human
keywords: RGS protein; clinical pathology; pancreatic neoplasms; prognosis