title: A fluorescent biosensor based on carbon dots-labeled oligodeoxyribonucleotide and graphene oxide for mercury (II) detection.
abstract: As the newest two members of the carbon materials family, carbon dots (CDs) and graphene oxide (GO) possess many excellent optical properties resulting in a wide range of applications. In this work, we successfully synthesized CDs with a high-quantum-yield, and labeled them on oligodeoxyribonucleotide (ODN). The fluorescence of resultant CDs-labeled oligodeoxyribonucleotide (ODN-CDs) was quenched by GO via fluorescence resonance energy transfer. In the presence of Hg(2+), the fluorescence was recovered by the release of ODN-CDs from GO due to the formation of T-Hg(2+)-T duplex. In the light of this theory, we designed a simple, highly sensitive and selective fluorometric Hg(2+) sensor based on CDs-labeled oligodeoxyribonucleotide and GO without complicated, costly and time-consuming operations. Under the optimal conditions, a linear relationship was obtained between relative fluorescence intensity and the concentration of Hg(2+) in the range of 5-200 nM (R(2)=0.974). The present GO-based sensor system is highly selective toward Hg(2+) over a wide range of metal ions and has a detection limit of 2.6 nM. This method is reliable, and has been successfully applied for the detection of Hg(2+) in practical samples.
authors.affiliation: College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, Liaoning, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Cui
authors.affiliation: College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, Liaoning, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, Liaoning, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.affiliation: College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, Liaoning, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Hou
authors.affiliation: College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, Liaoning, China.
authors.forename: Peiyao
authors.initials: P
authors.lastname: Wang
authors.affiliation: College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, Liaoning, China.
authors.forename: Zhenni
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, Liaoning, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D002244:Carbon / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation*; D006108:Graphite / Q000737:chemistry*; D008628:Mercury / Q000032:analysis* / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry; D045663:Quantum Dots; D013194:Staining and Labeling / Q000379:methods
chemical_list: D009838:Oligodeoxyribonucleotides; D010087:Oxides; D002244:Carbon; D006108:Graphite; D008628:Mercury
keywords: Carbon dots; Graphene oxide; Mercury detection; Oligodeoxyribonucleotide