title: Microfluidic device with integrated microfilter of conical-shaped holes for high efficiency and high purity capture of circulating tumor cells.
abstract: Capture of circulating tumor cells (CTCs) from peripheral blood of cancer patients has major implications for metastatic detection and therapy analyses. Here we demonstrated a microfluidic device for high efficiency and high purity capture of CTCs. The key novelty of this approach lies on the integration of a microfilter with conical-shaped holes and a micro-injector with cross-flow components for size dependent capture of tumor cells without significant retention of non-tumor cells. Under conditions of constant flow rate, tumor cells spiked into phosphate buffered saline could be recovered and then cultured for further analyses. When tumor cells were spiked in blood of healthy donors, they could also be recovered at high efficiency and high clearance efficiency of white blood cells. When the same device was used for clinical validation, CTCs could be detected in blood samples of cancer patients but not in that of healthy donors. Finally, the capture efficiency of tumor cells is cell-type dependent but the hole size of the filter should be more closely correlated to the nuclei size of the tumor cells. Together with the advantage of easy operation, low-cost and high potential of integration, this approach offers unprecedented opportunities for metastatic detection and cancer treatment monitoring.
authors.affiliation: 1] Ecole Normale Supérieure-PSL Research University, Département de Chimie, Sorbonne Universités - UPMC Univ Paris 06, CNRS UMR 8640 PASTEUR, 24, rue Lhomond, 75005 Paris, France [2].
authors.forename: Yadong
authors.initials: Y
authors.lastname: Tang
authors.affiliation: 1] BiocareCell, 249, rue Saint-Denis, 75002 Paris, France [2].
authors.forename: Jian
authors.initials: J
authors.lastname: Shi
authors.affiliation: Ecole Normale Supérieure-PSL Research University, Département de Chimie, Sorbonne Universités - UPMC Univ Paris 06, CNRS UMR 8640 PASTEUR, 24, rue Lhomond, 75005 Paris, France.
authors.forename: Sisi
authors.initials: S
authors.lastname: Li
authors.affiliation: Institut Curie, UMR 144 CNRS, 12 rue Lhomond, 75005 Paris, France.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: 1] BiocareCell, 249, rue Saint-Denis, 75002 Paris, France [2] Sorbonne Universités - UPMC Univ Paris 06, 4 place Jussieu 75005 Paris, France.
authors.forename: Yvon E
authors.initials: YE
authors.lastname: Cayre
authors.affiliation: 1] Ecole Normale Supérieure-PSL Research University, Département de Chimie, Sorbonne Universités - UPMC Univ Paris 06, CNRS UMR 8640 PASTEUR, 24, rue Lhomond, 75005 Paris, France [2] Institute for Integrated Cell-Material Science, Kyoto University, Kyoto 606-8507, Japan [3] Institute for Interdisciplinary Research, Jianghan University, 430056 Wuhan, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
mesh_terms: D045744:Cell Line, Tumor; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D002470:Cell Survival; D005374:Filtration / Q000295:instrumentation / Q000379:methods*; D019073:HT29 Cells; D006801:Humans; D017493:Leukocyte Common Antigens / Q000032:analysis / Q000097:blood; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D009369:Neoplasms / Q000473:pathology*; D009360:Neoplastic Cells, Circulating / Q000473:pathology*
chemical_list: D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human