title: Isolation and structural elucidation of novel antimicrobial compounds from maggots of Chrysomyis megacephala Fabricius.
abstract: The excretions/secretions from the maggot of Chrysomyis megacephala Fabricius are traditionally used to treat serious infections in China. In this study, bioassay-guided fractionation led to the isolation of three novel antibacterial compounds (1-3), including important fluorinated compounds (3 and 5), together with other nine known compounds from 70% methanol extract of C. megacephala. The structures of the new compounds were elucidated by NMR spectroscopic analysis and high-resolution mass spectroscopy. The antibacterial activities of the isolated compounds were evaluated using agar disc diffusion method. New compounds 1 and 2 exhibited moderate activity against Bacillus subtilis with a minimum inhibitory concentration (MIC) of 250 μg mL(- 1). The most active compounds 3 and 5 displayed a broad spectrum of antimicrobial activity with an MIC of 125 μg mL(- 1) against G(+) and G(- ) bacteria. The structure of the above-mentioned novel compounds and their antimicrobial activities are herein reported for the first time from the natural product of insects.
authors.affiliation: a School of Chemical Engineering and Pharmaceutics, Henan University of Science and Technology , Luoyang , Henan 471023 , P.R. China.
authors.forename: Jiayu
authors.initials: J
authors.lastname: Gao
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Li
authors.forename: Lanlan
authors.initials: L
authors.lastname: Niu
authors.forename: Ranran
authors.initials: R
authors.lastname: Cao
authors.forename: Weiping
authors.initials: W
authors.lastname: Yin
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001412:Bacillus subtilis / Q000187:drug effects; D001688:Biological Products / Q000737:chemistry; D002681:China; D004175:Diptera / Q000737:chemistry*; D007814:Larva / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure
chemical_list: D000900:Anti-Bacterial Agents; D001688:Biological Products
keywords: Chrysomyis megacephala Fabricius; antimicrobial activity; insect's natural products; maggot (Wuguchong); new compounds