title: Improvement of ligninolytic properties by recombinant expression of glyoxal oxidase gene in hyper lignin-degrading fungus Phanerochaete sordida YK-624.
abstract: Glyoxal oxidase (GLOX) is a source of the extracellular H2O2 required for the oxidation reactions catalyzed by the ligninolytic peroxidases. In the present study, the GLOX-encoding gene (glx) of Phanerochaete chrysosporium was cloned, and bee2 promoter of P. sordida YK-624 was used to drive the expression of glx. The expression plasmid was transformed into a P. sordida YK-624 uracil auxotrophic mutant (strain UV-64), and 16 clones were obtained as GLOX-introducing transformants. These transformants showed higher GLOX activities than wild-type P. sordida YK-624 and control transformants harboring marker plasmid. RT-PCR analysis indicated that the increased GLOX activity was associated with elevated recombinant glx expression. Moreover, these transformants showed higher ligninolytic activity than control transformants. These results suggest that the ligninolytic properties of white-rot fungi can be improved by recombinant expression of glx.
authors.affiliation: a Department of Applied Biological Chemistry , Graduate School of Agriculture, Shizuoka University , Shizuoka , Japan.
authors.forename: Yuto
authors.initials: Y
authors.lastname: Yamada
authors.forename: Jianqiao
authors.initials: J
authors.lastname: Wang
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kawagishi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Hirai
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D002999:Clone Cells; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D008031:Lignin / Q000737:chemistry / Q000378:metabolism*; D020075:Phanerochaete / Q000201:enzymology / Q000235:genetics*; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014169:Transformation, Bacterial
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins; D008031:Lignin; D000429:Alcohol Oxidoreductases; C052371:glyoxal oxidase
keywords: Phanerochaete sordida YK-624; glyoxal oxidase; lignin degradation; molecular breeding