title: Comparison of pulpal responses to pulpotomy and pulp capping with biodentine and mineral trioxide aggregate in dogs.
abstract: BACKGROUND
  This study evaluated the pulpal and periapical responses of dogs' teeth after pulpotomy and pulp capping with a new tricalcium silicate-based cement (Biodentine) when compared with mineral trioxide aggregate (MTA) by radiographic, histopathologic, and histomicrobiological analyses.
  
  
  METHODS
  Sixty roots (30 teeth) of dogs were divided into 2 groups, Biodentine (n = 36 roots) and ProRoot MTA (control, n = 24 roots). Animals were killed after 120 days, and the teeth were subjected to histotechnical processing (hematoxylin-eosin and Brown and Brenn staining). Qualitative and quantitative histopathologic data were analyzed by Fisher exact and Mann-Whitney tests (α = 0.05).
  
  
  RESULTS
  Radiographically, mineralized tissue bridge formation was observed in more specimens treated with Biodentine (96.8%) than with MTA (72.2%) (P = .02). Integrity of the lamina dura and absence of periapical bone rarefaction and root resorption (external and internal) were observed in all specimens. Histopathologic and histomicrobiological analyses revealed mineralized tissue bridge formation, pulpal vitality, odontoblast layer integrity, preserved periodontal ligament, and absence of bone or root resorption and microorganisms in both groups. Although the bridges formed at the amputation site had similar morphology, they were significantly thicker in the Biodentine group (P < .0001). Comparison between the radiographic and histopathologic results showed that radiographic visualization of more bridges in the Biodentine group was related to bridge thickness because radiographic diagnosis was flawed for bridges with thickness less than 0.5 mm. Fluorescence microscopy improved the visualization of bridge structure.
  
  
  CONCLUSIONS
  Biodentine presented tissue compatibility and allowed for mineralized tissue bridge formation after pulpotomy in all specimens with similar morphology and integrity to those formed with use of MTA. Periapical radiographs failed in detecting mineralized tissue bridges with thickness less than 0.5 mm.
authors.affiliation: University of São Paulo, School of Dentistry of Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil. Electronic address: andiara@forp.usp.br.
authors.forename: Andiara
authors.initials: A
authors.lastname: De Rossi
authors.affiliation: University of São Paulo, School of Dentistry of Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Lea Assed Bezerra
authors.initials: LA
authors.lastname: Silva
authors.affiliation: School of Dentistry, University of Barcelona, Barcelona, Spain.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Gatón-Hernández
authors.affiliation: School of Dentistry, University of Barcelona, Barcelona, Spain.
authors.forename: Manoel Damião
authors.initials: MD
authors.lastname: Sousa-Neto
authors.affiliation: University of São Paulo, School of Dentistry of Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Paulo
authors.initials: P
authors.lastname: Nelson-Filho
authors.affiliation: University of São Paulo, School of Dentistry of Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Raquel Assed Bezerra
authors.initials: RA
authors.lastname: Silva
authors.affiliation: University of São Paulo, School of Dentistry of Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Alexandra Mussolino
authors.initials: AM
authors.lastname: de Queiroz
mesh_terms: D017607:Aluminum Compounds / Q000627:therapeutic use*; D016301:Alveolar Bone Loss / Q000517:prevention & control; D000539:Alveolar Process / Q000000981:diagnostic imaging / Q000187:drug effects; D000818:Animals; D017610:Calcium Compounds / Q000627:therapeutic use*; D003782:Dental Pulp / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000503:physiopathology; D003809:Dentin, Secondary / Q000000981:diagnostic imaging / Q000187:drug effects; D004285:Dogs; D004338:Drug Combinations; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008856:Microscopy, Fluorescence; D009804:Odontoblasts / Q000187:drug effects / Q000473:pathology; D010087:Oxides / Q000627:therapeutic use*; D010486:Periapical Tissue / Q000000981:diagnostic imaging / Q000187:drug effects / Q000503:physiopathology; D010513:Periodontal Ligament / Q000000981:diagnostic imaging / Q000187:drug effects; D057872:Pulp Capping and Pulpectomy Agents / Q000627:therapeutic use*; D011672:Pulpotomy / Q000379:methods*; D016300:Radiography, Bitewing / Q000379:methods; D012391:Root Resorption / Q000517:prevention & control; D017640:Silicates / Q000627:therapeutic use*; D013997:Time Factors
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D057872:Pulp Capping and Pulpectomy Agents; D017640:Silicates; C086631:mineral trioxide aggregate; C506393:tricalcium silicate
keywords: Biodentine; mineral trioxide aggregate; pulpotomy