title: [Ox-LDL down-regulates expression of pigment epithelium-derived factor in human umbilical vein endothelial cells].
abstract: Pigment epithelium-derived factor (PEDF) is a multifunctional protein with anti-inflammatory, antioxidant and antithrombotic properties and plays a protective role against atherosclerosis (AS). The purpose of the present study is to explore the effects of oxidized low density lipoprotein (ox-LDL) on the expression of PEDF in cultured human umbilical vein endothelial cells (HUVECs). HUVECs were cultured and incubated with ox-LDL at different concentrations (6.25, 12.5, 25, 50, 100 and 150 mg/L) for 24 h. Apoptosis of endothelial cells were assayed by morphological staining and flow cytometry. The intracellular reactive oxygen species (ROS) levels were measured by flow cytometry. Cell viability was assayed by MTT assay. PEDF protein and mRNA expressions in HUVECs were analyzed by Western blot and quantitative real-time PCR, respectively. The results showed that ox-LDL significantly induced apoptosis, reduced cell viability, increased intracellular ROS levels and decreased the PEDF expression in HUVECs in a concentration-dependent manner. Ox-LDL at 50 mg/L obviously decreased the PEDF protein expression compared with control group (P < 0.05), whereas 25 mg/L ox-LDL already markedly reduced the PEDF mRNA expression (P < 0.05). In conclusion, the results suggest that ox-LDL down-regulates the PEDF expression through an increased ox-LDL-induced intracellular production of ROS.
authors.affiliation: Geriatrics Department, Civil Aviation General Hospital, Beijing 100123, China; Key Laboratory of Atherosclerosis in Universities of Shandong, Institute of Atherosclerosis, Taishan Medical University, Tai'an 271000, China; Second Affiliated Hospital of Qiqihar Medical College, Qiqihar 161000, China; Division of Cardiovascular Department, Chinese PLA General Hospital, Beijing 100853, China. zhuping_301@yahoo.com; shucunqin@hotmail.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Shu-Tong
authors.initials: ST
authors.lastname: Yao
authors.forename: Lei
authors.initials: L
authors.lastname: Zhai
authors.forename: Yue-Long
authors.initials: YL
authors.lastname: Feng
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Song
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
authors.forename: Shu-Cun
authors.initials: SC
authors.lastname: Qin
mesh_terms: D017209:Apoptosis; D002478:Cells, Cultured; D015536:Down-Regulation; D005136:Eye Proteins / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D009414:Nerve Growth Factors / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015843:Serpins / Q000378:metabolism*; D000099174:Pigment Epithelium-Derived Factor
chemical_list: D005136:Eye Proteins; D008077:Lipoproteins, LDL; D009414:Nerve Growth Factors; D017382:Reactive Oxygen Species; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor; C099252:oxidized low density lipoprotein