title: Altered microglial phagocytosis in GPR34-deficient mice.
abstract: GPR34 is a Gi/o protein-coupled receptor (GPCR) of the nucleotide receptor P2Y12 -like group. This receptor is highly expressed in microglia, however, the functional relevance of GPR34 in these glial cells is unknown. Previous results suggested an impaired immune response in GPR34-deficient mice infected with Cryptococcus neoformans. Here we show that GPR34 deficiency results in morphological changes in retinal and cortical microglia. RNA sequencing analysis of microglia revealed a number of differentially expressed transcripts involved in cell motility and phagocytosis. We found no differences in microglial motility after entorhinal cortex lesion and in response to laser lesion. However, GPR34-deficient microglia showed reduced phagocytosis activity in both retina and acutely isolated cortical slices. Our study identifies GPR34 as an important signaling component controlling microglial function, morphology and phagocytosis.
authors.affiliation: Institute of Biochemistry, Medical Faculty, University Leipzig, Leipzig, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Preissler
authors.forename: Antje
authors.initials: A
authors.lastname: Grosche
authors.forename: Vera
authors.initials: V
authors.lastname: Lede
authors.forename: Diana
authors.initials: D
authors.lastname: Le Duc
authors.forename: Katja
authors.initials: K
authors.lastname: Krügel
authors.forename: Vitali
authors.initials: V
authors.lastname: Matyash
authors.forename: Frank
authors.initials: F
authors.lastname: Szulzewsky
authors.forename: Sonja
authors.initials: S
authors.lastname: Kallendrusch
authors.forename: Kerstin
authors.initials: K
authors.lastname: Immig
authors.forename: Helmut
authors.initials: H
authors.lastname: Kettenmann
authors.forename: Ingo
authors.initials: I
authors.lastname: Bechmann
authors.forename: Torsten
authors.initials: T
authors.lastname: Schöneberg
authors.forename: Angela
authors.initials: A
authors.lastname: Schulz
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D039481:CD11b Antigen / Q000235:genetics / Q000378:metabolism; D000073883:CX3C Chemokine Receptor 1; D002465:Cell Movement / Q000235:genetics; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D017628:Microglia / Q000502:physiology*; D009924:Organ Culture Techniques; D010587:Phagocytosis / Q000235:genetics*; D019707:Receptors, Chemokine / Q000235:genetics / Q000378:metabolism; D049312:Receptors, Lysophospholipid / Q000172:deficiency* / Q000235:genetics; D012160:Retina / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D039481:CD11b Antigen; D000073883:CX3C Chemokine Receptor 1; C471885:Cx3cr1 protein, mouse; C118065:G-protein-coupled receptor 34; D019707:Receptors, Chemokine; D049312:Receptors, Lysophospholipid; D014409:Tumor Necrosis Factor-alpha; D049452:Green Fluorescent Proteins
keywords: GPCR; GPR34; OHSC; RNA sequencing; microglia; neurodegenerative disease; phagocytosis