title: Expression of CXCR1 (interleukin-8 receptor) in murine macrophages after staphylococcus aureus infection and its possible implication on intracellular survival correlating with cytokines and bacterial anti-oxidant enzymes.
abstract: Interaction with the live Staphylococcus aureus promotes secretion of interleukin-8 (IL-8), although the expressions of functional CXCR1 (IL-8RA) in murine macrophages have not been identified. Expression of CXCR1 was induced in S. aureus-infected macrophages, whereas, CXCR1 was undetectable in control macrophages. CXCR1 blocking significantly reduced the phagocytosis of S. aureus and TNF-α, IL-6, IL-1β, IFN-γ, IL-12, and IL-8 production and increased release of MIP-2 and soluble TNF-R1. Increased bacterial catalase and decreased superoxide dismutase (SOD) activities by S. aureus with concomitant decrease in hydrogen peroxide (H2O2), superoxide anion, and nitric oxide (NO) release were observed in case of prior CXCR1 blocking. In the presence of cytochalasin D, S. aureus-mediated induction of IL-8 was inhibited concomitant with decreased bacterial count suggesting that internalization of S. aureus was necessary for induction of IL-8. Shedding of TNF-R1 due to CXCR1 blocking after S. aureus inoculation was critical for neutralization of TNF-α signaling and arrests the inflammation.
authors.affiliation: Department of Physiology, Immunology Laboratory, University of Calcutta, University Colleges of Science and Technology, 92 APC Road, Calcutta, 700009, West Bengal, India, biswa_dev2@yahoo.com.
authors.forename: Biswadev
authors.initials: B
authors.lastname: Bishayi
authors.forename: Debasish
authors.initials: D
authors.lastname: Bandyopadhyay
authors.forename: Arnab
authors.initials: A
authors.lastname: Majhi
authors.forename: Rana
authors.initials: R
authors.lastname: Adhikary
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis; D002470:Cell Survival / Q000502:physiology; D016207:Cytokines / Q000096:biosynthesis*; D005786:Gene Expression Regulation; D017737:Macrophages, Peritoneal / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D023062:Receptors, Interleukin-8A / Q000096:biosynthesis*; D013203:Staphylococcal Infections / Q000378:metabolism* / Q000473:pathology; D013211:Staphylococcus aureus
chemical_list: D000975:Antioxidants; D001426:Bacterial Proteins; D016207:Cytokines; D023062:Receptors, Interleukin-8A