title: Highly sensitive assay for acetylcholinesterase activity and inhibition based on a specifically reactive photonic nanostructure.
abstract: Assays for acetylcholinesterase (AChE) with high sensitivity and high selectivity as well as facile manipulation have been urgently required in various fields. In this work, a reaction-based photonic strategy was developed for the efficient assay of AChE activity and inhibition based on the synergetic combination of the specific thiol-maleimide addition reaction with photonic porous structure. It was found that various applications including detection of AChE activity, measurement of the related enzymatic kinetics, and screening of inhibitors could be efficiently implemented using such strategy. Remarkably, the unique photonic nanostructure endows the constructed sensing platform with high sensitivity with a limit of detection (LOD) of 5 mU/mL for AChE activity, high selectivity, and self-reporting signaling. Moreover, the label-free solid film-based sensing approach described here has advantages of facile manipulation and bare-eye readout, compared with conventional liquid-phase methods, exhibiting promising potential in practical application for the AChE assay.
authors.affiliation: Key Lab of Organic Optoelectronics and Molecular Engineering, Department of Chemistry, Tsinghua University , Beijing 100084, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.forename: Xuesong
authors.initials: X
authors.lastname: Li
authors.forename: Jiecheng
authors.initials: J
authors.lastname: Cui
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Yue
authors.initials: Y
authors.lastname: Lan
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Guangtao
authors.initials: G
authors.lastname: Li
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D003102:Colloids / Q000737:chemistry; D057075:Enzyme Assays / Q000379:methods*; D005663:Furans / Q000138:chemical synthesis / Q000737:chemistry; D008301:Maleimides / Q000138:chemical synthesis / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D017785:Photons; D013057:Spectrum Analysis
chemical_list: D002800:Cholinesterase Inhibitors; D003102:Colloids; D005663:Furans; D008301:Maleimides; C043592:maleimide; D000110:Acetylcholinesterase
keywords: acetylcholinesterase; enzyme assay; photonic nanostructure; reaction-based biosensor; responsive polymer films