title: Enhanced angiogenic effect of adipose-derived stromal cell spheroid with low-level light therapy in hind limb ischemia mice.
abstract: The aim of this study was to investigate the effects of low-level laser therapy (LLLT) on transplanted human adipose-derived mesenchymal stem cells (hASCs) spheroid in a hind limb ischemia animal model. LLLT, hASCs spheroid and hASCs spheroid transplantation with LLLT (spheroid + LLLT) were applied to the ischemic hind limbs in athymic mice. The survival, differentiation and secretion of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (FGF), and hepatocyte growth factor (HGF) of the spheroid ASCs were evaluated by immunohistochemistry and western blots. Spheroid + LLLT group had enhanced the tissue regeneration, including angiogenesis, compared with the ASC group. The spheroid ASCs contributed to tissue regeneration via differentiation and secretion of growth factors. In the spheroid + LLLT group, the survival of spheroid hASCs increased with a concomitant decrease in apoptosis of spheroid hASCs in the ischemic hind limb. The secretion of growth factors was stimulated in the spheroid + LLLT group compared with the ASCs and spheroid group. These data suggested that LLLT is an effective biostimulator of spheroid hASCs in tissue regeneration that enhanced the survival of ASCs and stimulated the secretion of growth factors in the ischemic hind limb.
authors.affiliation: Beckman Laser Institute Korea, Dankook University, 119 Dandae-ro, Cheonan, Chungnam 330-714, South Korea.
authors.forename: In-Su
authors.initials: IS
authors.lastname: Park
authors.affiliation: Beckman Laser Institute Korea, Dankook University, 119 Dandae-ro, Cheonan, Chungnam 330-714, South Korea; Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Dankook University, 119 Dandae-ro, Cheonan, Chungnam 330-714, South Korea.
authors.forename: Phil-Sang
authors.initials: PS
authors.lastname: Chung
authors.affiliation: Beckman Laser Institute Korea, Dankook University, 119 Dandae-ro, Cheonan, Chungnam 330-714, South Korea; Department of Biomedical Science, Dankook University, Cheonan, Chungnam 330-714, South Korea; Biomedical Translational Research Institute, Dankook University, Cheonan, Chungnam 330-714, South Korea. Electronic address: jcahn@dankook.ac.kr.
authors.forename: Jin Chul
authors.initials: JC
authors.lastname: Ahn
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D043925:Angiogenesis Inducing Agents / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation; D002470:Cell Survival; D004195:Disease Models, Animal; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006614:Hindlimb / Q000378:metabolism / Q000473:pathology; D007511:Ischemia / Q000628:therapy*; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing
chemical_list: D043925:Angiogenesis Inducing Agents; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; D017228:Hepatocyte Growth Factor
keywords: Adipose-derived stromal cell; Angiogenesis; Endothelial differentiation; Low level light; Spheroid