title: Virus-like particles from Escherichia Coli-derived untagged papaya ringspot virus capsid protein purified by immobilized metal affinity chromatography enhance the antibody response against a soluble antigen.
abstract: There is a growing interest in using virus-like particles (VLPs) as scaffolds for the presentation of antigens of choice to the immune system. In this work, VLPs from papaya ringspot virus capsid protein expressed in Escherichia coli were evaluated as enhancers of antibody response against a soluble antigen. Interestingly, although the capsid protein lacks a histidine tag, its purification by immobilized metal affinity chromatography was achieved. The formation of VLPs was demonstrated by electron microscopy for the first time for this capsid protein. VLPs were enriched by polyethylene glycol precipitation. Additionally, these VLPs were chemically coupled to green fluorescent protein in order to evaluate them as antigen carriers; however, bioconjugate instability was observed. Nonetheless, the adjuvant effect of these VLPs on BALB/c mice was evaluated, using GFP as antigen, resulting in a significant increase in anti-GFP IgG response, particularly, IgG1 class, demonstrating that the VLPs enhance the immune response against the antigen chosen in this study.
authors.affiliation: Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Carretera Guadalajara-Nogales km 15.5, 45110, Zapopan, Jalisco, Mexico, guerreroajf@hotmail.com.
authors.forename: Jesús
authors.initials: J
authors.lastname: Guerrero-Rodríguez
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Manuel-Cabrera
authors.forename: Y Apatzingan
authors.initials: YA
authors.lastname: Palomino-Hermosillo
authors.forename: Paola Guadalupe
authors.initials: PG
authors.lastname: Delgado-Guzmán
authors.forename: Martha
authors.initials: M
authors.lastname: Escoto-Delgadillo
authors.forename: Laura
authors.initials: L
authors.lastname: Silva-Rosales
authors.forename: Sara Elisa
authors.initials: SE
authors.lastname: Herrera-Rodríguez
authors.forename: Carla
authors.initials: C
authors.lastname: Sánchez-Hernández
authors.forename: Abel
authors.initials: A
authors.lastname: Gutiérrez-Ortega
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D036022:Capsid Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D049452:Green Fluorescent Proteins / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D017800:Potyvirus / Q000276:immunology / Q000378:metabolism*; D058425:Vaccines, Virus-Like Particle / Q000737:chemistry / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D058425:Vaccines, Virus-Like Particle; D049452:Green Fluorescent Proteins