title: Cell cycle-dependent O-GlcNAc modification of tobacco histones and their interaction with the tobacco lectin.
abstract: The Nicotiana tabacum agglutinin or Nictaba is a nucleocytoplasmic lectin that is expressed in tobacco after the plants have been exposed to jasmonate treatment or insect herbivory. Nictaba specifically recognizes GlcNAc residues. Recently, it was shown that Nictaba is interacting in vitro with the core histone proteins from calf thymus. Assuming that plant histones - similar to their animal counterparts - undergo O-GlcNAcylation, this interaction presumably occurs through binding of the lectin to the O-GlcNAc modification present on the histones. Hereupon, the question was raised whether this modification also occurs in plants and if it is cell cycle dependent. To this end, histones were purified from tobacco BY-2 suspension cells and the presence of O-GlcNAc modifications was checked. Concomitantly, O-GlcNAcylation of histone proteins was studied. Our data show that similar to animal histones plant histones are modified by O-GlcNAc in a cell cycle-dependent fashion. In addition, the interaction between Nictaba and tobacco histones was confirmed using lectin chromatography and far Western blot analysis. Collectively these findings suggest that Nictaba can act as a modulator of gene transcription through its interaction with core histones.
authors.affiliation: Ghent University, Dept. Molecular Biotechnology, Lab Biochemistry and Glycobiology, Coupure Links 653, B-9000 Gent, Belgium.
authors.forename: Annelies
authors.initials: A
authors.lastname: Delporte
authors.affiliation: Ghent University, Dept. Molecular Biotechnology, Lab Biochemistry and Glycobiology, Coupure Links 653, B-9000 Gent, Belgium.
authors.forename: Jeroen
authors.initials: J
authors.lastname: De Zaeytijd
authors.affiliation: Ghent University, Dept. Plant Production, Coupure Links 653, B-9000 Gent, Belgium.
authors.forename: Nico
authors.initials: N
authors.lastname: De Storme
authors.affiliation: University of Antwerp, Dept. of Biology, Laboratory of Molecular Plant and Biotechnology (MPB), Centre for Proteome Analysis and Mass Spectrometry (CeProMa), Belgium.
authors.forename: Abdelkrim
authors.initials: A
authors.lastname: Azmi
authors.affiliation: Ghent University, Dept. Plant Production, Coupure Links 653, B-9000 Gent, Belgium.
authors.forename: Danny
authors.initials: D
authors.lastname: Geelen
authors.affiliation: Ghent University, Dept. Crop Protection, Coupure Links 653, B-9000 Gent, Belgium.
authors.forename: Guy
authors.initials: G
authors.lastname: Smagghe
authors.affiliation: University of Antwerp, Dept. of Biology, Laboratory of Molecular Plant and Biotechnology (MPB), Centre for Proteome Analysis and Mass Spectrometry (CeProMa), Belgium.
authors.forename: Yves
authors.initials: Y
authors.lastname: Guisez
authors.affiliation: Ghent University, Dept. Molecular Biotechnology, Lab Biochemistry and Glycobiology, Coupure Links 653, B-9000 Gent, Belgium. Electronic address: elsjm.vandamme@ugent.be.
authors.forename: Els J M
authors.initials: EJ
authors.lastname: Van Damme
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism*; D002453:Cell Cycle; D006657:Histones / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D014026:Nicotiana / Q000378:metabolism*
chemical_list: D006657:Histones; D037102:Lectins; D000117:Acetylglucosamine
keywords: Cell cycle; Histone; Nictaba; O-GlcNAc; Protein interaction; Tobacco