title: Comparative study of corneal endothelial cell damage after femtosecond laser assisted deep stromal dissection.
abstract: OBJECTIVE
  To find a relatively safe designed stromal bed thickness to avoid endothelial damage for lamellar keratoplasty with an Allegretto Wavelight FS200 femtosecond laser.
  
  
  METHODS
  Twelve rabbits were randomly divided into 50 μm and 150 μm groups according to the anticipated residue stromal bed thickness preparation with a femtosecond laser. Six rabbits without laser cutting were used as a control group. Central endothelial images were analyzed with in vivo confocal microscopy and scanning electron microscopy. The apoptosis of endothelium was evaluated with Hoechst 33342 staining and a TUNEL assay.
  
  
  RESULTS
  The endothelium of the 50 μm group had extensive injuries upon in vivo confocal and scanning electron microscopic observation, and minor injuries were observed in the 150 μm group. Moreover, more apoptotic cells were observed in the 50 μm group.
  
  
  CONCLUSIONS
  When using a FS200 femtosecond laser assisted anterior lamellar keratoplasty, there was minor endothelium damage with a 150 μm stromal bed, and a more than 150 μm thickness stromal bed design may prevent the damage of corneal endothelium.
authors.affiliation: State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, No. 5 Yan'erdao Road, Qingdao 266071, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.affiliation: State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, No. 5 Yan'erdao Road, Qingdao 266071, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, No. 5 Yan'erdao Road, Qingdao 266071, China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Sun
authors.affiliation: State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, No. 5 Yan'erdao Road, Qingdao 266071, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Sui
authors.affiliation: State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, No. 5 Yan'erdao Road, Qingdao 266071, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, No. 5 Yan'erdao Road, Qingdao 266071, China.
authors.forename: Dongfang
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, No. 5 Yan'erdao Road, Qingdao 266071, China.
authors.forename: Zhaoli
authors.initials: Z
authors.lastname: Chen
authors.affiliation: State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, No. 5 Yan'erdao Road, Qingdao 266071, China.
authors.forename: Hua
authors.identifier: 0000-0002-1326-6946
authors.initials: H
authors.lastname: Gao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D055954:Corneal Endothelial Cell Loss / Q000473:pathology*; D016039:Corneal Transplantation; D004210:Dissection / Q000009:adverse effects* / Q000295:instrumentation* / Q000379:methods; D004728:Endothelium, Corneal / Q000473:pathology / Q000528:radiation effects / Q000601:surgery; D005260:Female; D006651:Histocytochemistry; D020287:In Situ Nick-End Labeling; D007834:Lasers; D008297:Male; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D066167:Slit Lamp