title: Extracellular DNA traps in bronchoalveolar fluid from a murine eosinophilic pulmonary response.
abstract: Asthma is associated with a loss of the structural integrity of airway epithelium and dysfunction of the physical barrier, which protects airways from external harmful factors. Granulocyte activation causes the formation of extracellular traps, releasing web-like structures of DNA and proteins, being important to kill pathogens extracellularly. We investigated whether eosinophils infiltrating airways in an experimental model of asthma would induce eosinophil extracellular traps (EETs) in bronchoalveolar lavage fluid and lung tissue. We showed that an ovalbumin (OVA) asthma protocol presented a significant increase in eosinophil counts with increased extracellular DNA in bronchoalveolar lavage fluid as well as in lung tissue, confirming the presence of DNA traps colocalized with eosinophil peroxidase. EETs formation was reversed by DNase treatment. With these approaches, we demonstrated for the first time that OVA-challenged mice release extracellular DNA traps, which could aggravate pulmonary dysfunction.
authors.affiliation: Laboratory of Pediatric Respirology, Infant Centre, Institute of Biomedical Research (IPB), Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.
authors.forename: A A
authors.initials: AA
authors.lastname: Cunha
authors.forename: B N
authors.initials: BN
authors.lastname: Porto
authors.forename: N K
authors.initials: NK
authors.lastname: Nuñez
authors.forename: R G
authors.initials: RG
authors.lastname: Souza
authors.forename: M H M
authors.initials: MH
authors.lastname: Vargas
authors.forename: J S
authors.initials: JS
authors.lastname: Silveira
authors.forename: T T R
authors.initials: TT
authors.lastname: Souza
authors.forename: N
authors.initials: N
authors.lastname: Jaeger
authors.forename: P M
authors.initials: PM
authors.lastname: Pitrez
mesh_terms: D000818:Animals; D001249:Asthma / Q000276:immunology / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology*; D004247:DNA / Q000378:metabolism*; D004195:Disease Models, Animal; D065206:Extracellular Traps / Q000276:immunology* / Q000378:metabolism*; D007249:Inflammation / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D051379:Mice; D010047:Ovalbumin / Q000009:adverse effects; D011657:Pulmonary Eosinophilia / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology
chemical_list: D010047:Ovalbumin; D004247:DNA
keywords: asthma; eosinophils; extracellular DNA traps