title: Overexpression of EZH2 associates with a poor prognosis in chronic lymphocytic leukemia.
abstract: EZH2, a histone methyltransferase, is overexpressed in several human tumors, but whether it exerts any impact in chronic lymphocytic leukemia (CLL) remains unknown. We used real time PCR to investigate the expression profile of EZH1 and EZH2 in 59 CLL patients, 10 samples of purified B-cells from healthy donors and 12 normal adult tissues. EZH2 was overexpressed in CLL patients and correlates with high white blood cell count, ZAP-70 expression and chromosomal abnormalities. EHZ1 expression does not correlate with CLL progression. EZH2 overexpression is related to a poor prognosis of CLL and could be a useful tool to assess its aggressiveness.
authors.affiliation: Laboratory of Molecular Pathology of Cancer, University of Brasília, Av. L2 Norte, Brasília, DF 70.910-900, Brazil.
authors.forename: Doralina do Amaral
authors.initials: Ddo A
authors.lastname: Rabello
authors.affiliation: Department of Internal Medicine, Medical School of Ribeirão Preto, University of São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, SP 14.048-900, Brazil.
authors.forename: Antonio Roberto
authors.initials: AR
authors.lastname: Lucena-Araujo
authors.affiliation: Laboratory of Molecular Pathology of Cancer, University of Brasília, Av. L2 Norte, Brasília, DF 70.910-900, Brazil.
authors.forename: Juliana Carvalho Rocha
authors.initials: JC
authors.lastname: Alves-Silva
authors.affiliation: Laboratory of Molecular Pathology of Cancer, University of Brasília, Av. L2 Norte, Brasília, DF 70.910-900, Brazil.
authors.forename: Vinícius Burnett Aboud Souza
authors.initials: VB
authors.lastname: da Eira
authors.affiliation: Hospital de Base do Distrito Federal, Setor Hospitalar Sul, Área Especial, Quadra 101, Brasília, DF 70.330-150, Brazil.
authors.forename: Maria Catarina Cals
authors.initials: MC
authors.lastname: de Vasconcellos
authors.affiliation: Department of Internal Medicine, Medical School of Ribeirão Preto, University of São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, SP 14.048-900, Brazil.
authors.forename: Fábio Morato
authors.initials: FM
authors.lastname: de Oliveira
authors.affiliation: Department of Internal Medicine, Medical School of Ribeirão Preto, University of São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, SP 14.048-900, Brazil.
authors.forename: Eduardo Magalhaes
authors.initials: EM
authors.lastname: Rego
authors.affiliation: Laboratory of Molecular Pathology of Cancer, University of Brasília, Av. L2 Norte, Brasília, DF 70.910-900, Brazil.
authors.forename: Felipe
authors.initials: F
authors.lastname: Saldanha-Araujo
authors.affiliation: Laboratory of Molecular Pathology of Cancer, University of Brasília, Av. L2 Norte, Brasília, DF 70.910-900, Brazil. Electronic address: pittella@unb.br.
authors.forename: Fábio
authors.initials: F
authors.lastname: Pittella Silva
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000071221:Enhancer of Zeste Homolog 2 Protein; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis* / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D063151:Polycomb Repressive Complex 2 / Q000096:biosynthesis*; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D051746:ZAP-70 Protein-Tyrosine Kinase / Q000096:biosynthesis
chemical_list: C103523:EZH1 protein, human; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; D063151:Polycomb Repressive Complex 2; D051746:ZAP-70 Protein-Tyrosine Kinase
keywords: Chronic lymphocytic leukemia; Cytogenetic; EZH2; Gene expression; Histone methyltransferase