title: Residues of deoxynivalenol (DON) and its metabolite de-epoxy-DON in eggs, plasma and bile of laying hens of different genetic backgrounds.
abstract: In the present study, the potential for carry-over of deoxynivalenol (DON) into eggs and DON residues in plasma and bile of laying hens of different genetic backgrounds after long-term feeding trial was investigated. A total of 80, 23-week-old laying hens were assigned to a feeding trial with two diets, a control diet and a Fusarium toxin-contaminated diet (FUS) (0.4 and 9.9 mg DON kg(-1), respectively). In the 60th week of hen's life, 10 eggs from each group were collected. In the 70th week of hen's life, all hens were slaughtered and samples of blood and bile were collected. The samples were analysed by liquid chromatography tandem mass spectrometry (LC-MS/MS) for DON and de-epoxy-DON. DON was only detected in samples of hens which fed the FUS diet while none of the samples analysed had detectable levels of de-epoxy-DON. In plasma and bile samples, DON levels ranged from 0.2 to 0.6 ng ml(-1) and from 1.8 to 4.1 ng ml(-1), respectively. DON levels in egg yolk and albumen ranged between 0.0-0.46 ng g(-1) and 0.0-0.35 ng g(-1), respectively, corresponding to carry-over rates of DON into eggs from 0.0 to 0.000016. Moreover, no differences in DON levels or carry-over rates were noticed between the two tested breeds. These results show that very low levels of DON were transferred into eggs and indicate that although eggs could contribute to human exposure to DON, the levels are very low and insignificant.
authors.affiliation: a Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health , Braunschweig , Germany.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ebrahem
authors.forename: Susanne
authors.initials: S
authors.lastname: Kersten
authors.forename: Hana
authors.initials: H
authors.lastname: Valenta
authors.forename: Gerhard
authors.initials: G
authors.lastname: Breves
authors.forename: Sven
authors.initials: S
authors.lastname: Dänicke
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D001646:Bile / Q000737:chemistry; D001947:Breeding; D002645:Chickens / Q000235:genetics / Q000378:metabolism*; D004531:Eggs / Q000032:analysis; D005260:Female; D005506:Food Contamination / Q000032:analysis; D005670:Fusarium; D009183:Mycotoxins / Q000008:administration & dosage; D053719:Tandem Mass Spectrometry / Q000662:veterinary; D014255:Trichothecenes / Q000097:blood / Q000737:chemistry*
chemical_list: D009183:Mycotoxins; D014255:Trichothecenes; C048238:deepoxy-deoxynivalenol; C007262:deoxynivalenol
keywords: Fusarium; breed differences; carry-over; deoxynivalenol; eggs; laying hens; mycotoxins