title: Ischemic preconditioning and atenolol on lung injury after intestinal ischemia and reperfusion in rats.
abstract: The aim of this study was evaluate the beta blocker atenolol (AT) and ischemic preconditioning (IPC) strategies for tissue protection against systemic effects of intestinal ischemia (I) and reperfusion (R) injury. Forty-two rats were pretreated with AT (1.5 mg · kg(-1)), 0.9% saline solution (SS; 0.1 mL), or IPC and then subjected to prolonged occlusion of the superior mesenteric artery for 60 minutes leading to I followed or not by 120 minutes of R, according to the group. For IPC, 5 minutes of I prior to 10 minutes of R were established. After this process of I or I-R, the right lung of each animal was adequately prepared for staining with hematoxylin and eosin and subsequent histologic analysis for quantification of inflammatory infiltrate was done. The left lung was frozen and prepared for assessment of oxidative stress by the quantification of thiobarbituric acid-reactivity substances (TBARS). Histologic analysis showed an important inflammatory infiltrate in the I-R + SS (I-R + SS = 4.5), which was significantly (P < .05) reduced by IPC (I-R + IPC = 3.0) or AT (I-R + AT = 3.0). Likewise, the TBARS levels were decreased by both strategies (I-R + SS = 0.63; I-R + IPC = 0.23; I-R + AT = 0.38; P < .05). Our results showed that AT and IPC attenuate pulmonary lesions caused by intestinal I and R process.
authors.affiliation: Escola Paulista de Medicina, Federal University of São Paulo (UNIFESP), Brazil.
authors.forename: W G S
authors.initials: WG
authors.lastname: Bonservizi
authors.affiliation: Laboratory of Clinical Emergencies (LIM-51), Brazil.
authors.forename: M K
authors.initials: MK
authors.lastname: Koike
authors.affiliation: Escola Paulista de Medicina, Federal University of São Paulo (UNIFESP), Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Saurim
authors.affiliation: Escola Paulista de Medicina, Federal University of São Paulo (UNIFESP), Brazil.
authors.forename: G A A
authors.initials: GA
authors.lastname: Felix
authors.affiliation: Escola Paulista de Medicina, Federal University of São Paulo (UNIFESP), Brazil.
authors.forename: S M
authors.initials: SM
authors.lastname: da Silva
authors.affiliation: Escola Paulista de Medicina, Federal University of São Paulo (UNIFESP), Brazil; Laboratory of Surgical Physiopathology (LIM 62), Department of Surgery, University of São Paulo, São Paulo, Brazil. Electronic address: edna.montero@gmail.com.
authors.forename: E F S
authors.initials: EF
authors.lastname: Montero
authors.affiliation: Escola Paulista de Medicina, Federal University of São Paulo (UNIFESP), Brazil.
authors.forename: M O
authors.initials: MO
authors.lastname: Taha
mesh_terms: D058671:Adrenergic beta-1 Receptor Antagonists / Q000494:pharmacology; D000818:Animals; D001262:Atenolol / Q000494:pharmacology*; D004195:Disease Models, Animal; D007422:Intestines / Q000098:blood supply*; D019194:Ischemic Preconditioning / Q000379:methods*; D008168:Lung / Q000098:blood supply*; D055370:Lung Injury / Q000209:etiology / Q000517:prevention & control*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications / Q000517:prevention & control*
chemical_list: D058671:Adrenergic beta-1 Receptor Antagonists; D001262:Atenolol