title: Development of an organic lateral resolution test device for imaging mass spectrometry.
abstract: An organic lateral resolution test device has been developed to measure the performance of imaging mass spectrometry (IMS) systems. The device contains periodic gratings of polyethylene glycol (PEG) and lipid bars covering a wide range of spatial frequencies. Microfabrication technologies were employed to produce well-defined chemical interfaces, which allow lateral resolution to be assessed using the edge-spread function (ESF). In addition, the design of the device allows for the direct measurement of the modulation transfer function (MTF) to assess image quality. Scanning electron microscopy (SEM) and time-of-flight secondary ion mass spectrometry (TOF-SIMS) were used to characterize the device. TOF-SIMS imaging was used to measure the chemical displacement of biomolecules in matrix-assisted laser desorption/ionization (MALDI) matrix crystals. In a proof-of-concept experiment, the platform was also used to evaluate MALDI matrix application methods, specifically aerosol spray and sublimation methods.
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg , SE-405 30, Gothenburg, Sweden.
authors.forename: Melissa K
authors.initials: MK
authors.lastname: Passarelli
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Amir Saeid
authors.initials: AS
authors.lastname: Mohammadi
authors.forename: Raphaël
authors.initials: R
authors.lastname: Trouillon
authors.forename: Ian
authors.initials: I
authors.lastname: Gilmore
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Ewing
mesh_terms: D002784:Cholesterol / Q000737:chemistry; D002857:Chromium / Q000737:chemistry; D003460:Crystallization; D055616:Microtechnology; D057054:Molecular Imaging / Q000295:instrumentation* / Q000379:methods; D010713:Phosphatidylcholines / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011791:Quartz / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation; D018629:Spectrometry, Mass, Secondary Ion / Q000295:instrumentation
chemical_list: D010713:Phosphatidylcholines; D002857:Chromium; D011791:Quartz; D011092:Polyethylene Glycols; D002784:Cholesterol