title: Total transcriptome, proteome, and allergome of Johnson grass pollen, which is important for allergic rhinitis in subtropical regions.
abstract: BACKGROUND
  Genomic data are lacking for many allergen sources. To circumvent this limitation, we implemented a strategy to reveal the repertoire of pollen allergens of a grass with clinical importance in subtropical regions, where an increasing proportion of the world's population resides.
  
  
  OBJECTIVE
  We sought to identify and immunologically characterize the allergenic components of the Panicoideae Johnson grass pollen (JGP; Sorghum halepense).
  
  
  METHODS
  The total pollen transcriptome, proteome, and allergome of JGP were documented. Serum IgE reactivities with pollen and purified allergens were assessed in 64 patients with grass pollen allergy from a subtropical region.
  
  
  RESULTS
  Purified Sor h 1 and Sor h 13 were identified as clinically important allergen components of JGP with serum IgE reactivity in 49 (76%) and 28 (43.8%), respectively, of patients with grass pollen allergy. Within whole JGP, multiple cDNA transcripts and peptide spectra belonging to grass pollen allergen families 1, 2, 4, 7, 11, 12, 13, and 25 were identified. Pollen allergens restricted to subtropical grasses (groups 22-24) were also present within the JGP transcriptome and proteome. Mass spectrometry confirmed the IgE-reactive components of JGP included isoforms of Sor h 1, Sor h 2, Sor h 13, and Sor h 23.
  
  
  CONCLUSIONS
  Our integrated molecular approach revealed qualitative differences between the allergenic components of JGP and temperate grass pollens. Knowledge of these newly identified allergens has the potential to improve specific diagnosis and allergen immunotherapy treatment for patients with grass pollen allergy in subtropical regions and reduce the burden of allergic respiratory disease globally.
authors.affiliation: School of Agriculture and Food Sciences, The University of Queensland, Brisbane, Australia.
authors.forename: Bradley C
authors.initials: BC
authors.lastname: Campbell
authors.affiliation: School of Agriculture and Food Sciences, The University of Queensland, Brisbane, Australia; Institute for Molecular Biosciences, The University of Queensland, Brisbane, Australia.
authors.forename: Edward K
authors.initials: EK
authors.lastname: Gilding
authors.affiliation: School of Medicine, Translational Research Institute, The University of Queensland, Brisbane, Australia.
authors.forename: Victoria
authors.initials: V
authors.lastname: Timbrell
authors.affiliation: School of Medicine, Translational Research Institute, The University of Queensland, Brisbane, Australia.
authors.forename: Preethi
authors.initials: P
authors.lastname: Guru
authors.affiliation: The University of Queensland Diamantina Institute, Translational Research Institute, The University of Queensland, Brisbane, Australia.
authors.forename: Dorothy
authors.initials: D
authors.lastname: Loo
authors.affiliation: Centri Associati di Allergologia Molecolare, Rome, Italy.
authors.forename: Danila
authors.initials: D
authors.lastname: Zennaro
authors.affiliation: Centri Associati di Allergologia Molecolare, Rome, Italy; Allergy Data Laboratories s.c., Latina, Italy.
authors.forename: Adriano
authors.initials: A
authors.lastname: Mari
authors.affiliation: Watkins Medical Centre, Brisbane, Australia.
authors.forename: Graham
authors.initials: G
authors.lastname: Solley
authors.affiliation: The University of Queensland Diamantina Institute, Translational Research Institute, The University of Queensland, Brisbane, Australia.
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Hill
authors.affiliation: School of Agriculture and Food Sciences, The University of Queensland, Brisbane, Australia.
authors.forename: Ian D
authors.initials: ID
authors.lastname: Godwin
authors.affiliation: School of Medicine, Translational Research Institute, The University of Queensland, Brisbane, Australia. Electronic address: j.davies2@uq.edu.au.
authors.forename: Janet M
authors.initials: JM
authors.lastname: Davies
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology*; D052179:Antigens, Plant / Q000276:immunology; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D008875:Middle Aged; D010936:Plant Extracts / Q000494:pharmacology; D010940:Plant Proteins / Q000276:immunology; D011058:Pollen / Q000276:immunology*; D020543:Proteome; D065631:Rhinitis, Allergic / Q000097:blood / Q000276:immunology*; D012882:Skin Tests; D045868:Sorghum / Q000276:immunology*; D059467:Transcriptome; D014329:Tropical Climate
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D010936:Plant Extracts; D010940:Plant Proteins; D020543:Proteome; D007073:Immunoglobulin E
keywords: Allergome; IgE; Johnson grass pollen; allergen components; allergic rhinitis; pollen allergy; proteome; transcriptome