title: Effect of conditioned medium from preameloblasts on regenerative cellular differentiation of the immature teeth with necrotic pulp and apical periodontitis.
abstract: BACKGROUND
  The purpose of this study was to investigate the effect of conditioned medium (CM) from murine preameloblasts on the cellular differentiation of mesenchymal stem cells (MSCs) in immature teeth with necrotic pulp and apical periodontitis.
  
  
  METHODS
  Pulp necrosis and apical periodontitis were induced in 30 immature permanent double-rooted premolars of 3 beagles and were randomly assigned to the following treatment groups: group CM (n = 10), revascularization treatment was performed using CM from preameloblasts of C57BL/6 mice apical bud cells; group CR (n = 10), conventional revascularization treatment was performed; positive control group (n = 5), left infected; and negative control group (n = 5), untreated. The dogs were followed up for 12 weeks and assessed for treatment outcomes with radiographic and histologic analyses. The effect of the CM on sequential Runx2 and osterix messenger RNA gene expression during the differentiation of MG63 osteoblastlike cells was analyzed with real-time polymerase chain reaction.
  
  
  RESULTS
  The overall treatment outcomes were not significantly different between the 2 treatment groups. However, the teeth in the CM group showed significantly more mature apices and a higher degree of hard tissue formation with projections intercalating into the pre-existing root dentin (P < .05). In CM-treated teeth, regenerated pulplike tissue was more frequently observed (P < .05). During differentiation, the CM induced early peak expression of Runx2 followed by sustained osterix overexpression.
  
  
  CONCLUSIONS
  CM from preameloblasts rendered a favorable effect in providing a physiologic microenvironment for the differentiation of MSCs after revascularization treatment.
authors.affiliation: Department of Conservative Dentistry, Seoul National University Dental Hospital, School of Dentistry, Dental Research Institute, Seoul National University, Jongro-Gu, Seoul, Korea.
authors.forename: Yeon-Jee
authors.initials: YJ
authors.lastname: Yoo
authors.affiliation: Department of Conservative Dentistry, Seoul National University Dental Hospital, School of Dentistry, Dental Research Institute, Seoul National University, Jongro-Gu, Seoul, Korea.
authors.forename: WooCheol
authors.initials: W
authors.lastname: Lee
authors.affiliation: Department of Maxillofacial Tissue Regeneration, School of Dentistry, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, Korea.
authors.forename: Young-Ah
authors.initials: YA
authors.lastname: Cho
authors.affiliation: Department of Oral Histology-Developmental Biology and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea.
authors.forename: Joo-Cheol
authors.initials: JC
authors.lastname: Park
authors.affiliation: Department of Conservative Dentistry, Seoul National University Dental Hospital, School of Dentistry, Dental Research Institute, Seoul National University, Jongro-Gu, Seoul, Korea.
authors.forename: Won-Jun
authors.initials: WJ
authors.lastname: Shon
authors.affiliation: Department of Conservative Dentistry, Seoul National University Dental Hospital, School of Dentistry, Dental Research Institute, Seoul National University, Jongro-Gu, Seoul, Korea. Electronic address: shbaek@snu.ac.kr.
authors.forename: Seung-Ho
authors.initials: SH
authors.lastname: Baek
mesh_terms: D000565:Ameloblasts / Q000502:physiology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D056624:Apexification / Q000379:methods; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D060833:Cellular Microenvironment / Q000502:physiology; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis; D017077:Culture Media, Conditioned; D003739:Dental Cementum / Q000473:pathology / Q000503:physiopathology; D003782:Dental Pulp / Q000473:pathology / Q000503:physiopathology; D003790:Dental Pulp Necrosis / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D003804:Dentin / Q000473:pathology / Q000503:physiopathology; D004285:Dogs; D059630:Mesenchymal Stem Cells / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D010006:Osteoblasts / Q000502:physiology; D010485:Periapical Periodontitis / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D011897:Random Allocation; D012038:Regeneration / Q000502:physiology; D018915:Root Canal Preparation / Q000379:methods; D013997:Time Factors; D054457:Tissue Scaffolds; D019227:Tooth Apex / Q000473:pathology / Q000503:physiopathology; D014157:Transcription Factors / Q000032:analysis; D016335:Zinc Fingers
chemical_list: D000900:Anti-Bacterial Agents; D050659:Core Binding Factor Alpha 1 Subunit; D017077:Culture Media, Conditioned; D014157:Transcription Factors
keywords: Cellular differentiation; Runx2; conditioned medium; osterix; preameloblast; revascularization