title: [Comparative analysis of collecting mononuclear cells from peripheral blood by using Fenwal CS-3000 plus, haemonetics MCS plus and COBE spectra separators].
abstract: This study was aimed to compare the collection efficiency of mononuclear cells (MNC) from peripheral blood as well as the changes of blood-related indices in patient by using 3 cell separators. MNC were collected from 94 tumor patients by using Fenwal CS-3000plus, Haemonetics MCSplus and COBE spectra separators. Routine blood test was performed before and after MNC collection to detect the potential effects of cell separators on blood-related indices in the patients. MNC count was performed. The percentages of CD3(+), CD4(+) and CD8(+) in peripheral blood cells were determined. The results showed that the MNC counts were (3.08 ± 0.79)×10(9), (3.21 ± 1.12)×10(9), and (3.22 ± 1.84)×10(9) per bag by CS-3000plus, MCSplus and COBE spectra, respectively. And the corresponding decrease of platelet percentage was (6.86 ± 5.70)%, (8.05 ± 5.14)% and (5.89 ± 4.48)%, respectively. The CD3, CD4 and CD8 ratios in peripheral blood of patients before and after treatment were significantly statistical different (P < 0.001). It is concluded that the MNC collection can be performed successfully with CS-3000plus, MCSplus and COBE spectra, and their collections can meet the needs in clinic.
authors.affiliation: Department of Blood Transfusion, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China. E-mail: zhouweiling0214@126.com.
authors.forename: Wen-Ling
authors.initials: WL
authors.lastname: Zhou
authors.affiliation: Department of Blood Transfusion, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Hao
mesh_terms: D000368:Aged; D002469:Cell Separation / Q000379:methods*; D055612:Cytokine-Induced Killer Cells / Q000166:cytology*; D003713:Dendritic Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation*; D008297:Male; D008875:Middle Aged