title: High performance characterization of triacylglycerols in milk and milk-related samples by liquid chromatography and mass spectrometry.
abstract: In this work, ultra high performance liquid chromatography was used for the characterization of non polar lipids (triacylglycerols) in milk samples of different origin, as well as milk-derivatives. For tackling such a task, three core-shell type octadecylsilica columns were serially coupled, reaching a total stationary phase length of 45cm, using acetonitrile-isopropanol gradient elution allowing triacylglicerol separation according to increasing partition number. The employment of an ion-trap-time-of-flight detection in conjunction with atmospheric-pressure chemical ionization mass spectrometry was carried out to positively identify a number of 243 different triacylglycerols containing up to 22 fatty acids, with 2-22 carbon atom alkyl chain length, and 0-3 double bonds. This work reports an extensive characterization of the triacylglycerol fraction in milk and milk-related samples of different sources.
authors.affiliation: Dipartimento di Scienze del Farmaco e dei Prodotti per la Salute (S.C.I.F.A.R.), University of Messina, Viale Annunziata, 98168 Messina, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Beccaria
authors.affiliation: Dipartimento di Scienze del Farmaco e dei Prodotti per la Salute (S.C.I.F.A.R.), University of Messina, Viale Annunziata, 98168 Messina, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Sullini
authors.affiliation: Dipartimento di Scienze dell'Ambiente, della Sicurezza, del Territorio, degli Alimenti e della Salute (S.A.S.T.A.S.), University of Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy; Chromaleont s.r.l. A start-up of the University of Messina, c/o Dipartimento di Scienze del Farmaco e dei Prodotti per la Salute (S.C.I.F.A.R.), University of Messina, Viale Annunziata, 98168 Messina, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Cacciola
authors.affiliation: Dipartimento di Scienze del Farmaco e dei Prodotti per la Salute (S.C.I.F.A.R.), University of Messina, Viale Annunziata, 98168 Messina, Italy; Chromaleont s.r.l. A start-up of the University of Messina, c/o Dipartimento di Scienze del Farmaco e dei Prodotti per la Salute (S.C.I.F.A.R.), University of Messina, Viale Annunziata, 98168 Messina, Italy; Centro Integrato di Ricerca (C.I.R.), University Campus Bio-Medico of Rome, Via Álvaro del Portillo 21, 00128 Rome, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Donato
authors.affiliation: Dipartimento di Scienze del Farmaco e dei Prodotti per la Salute (S.C.I.F.A.R.), University of Messina, Viale Annunziata, 98168 Messina, Italy; Chromaleont s.r.l. A start-up of the University of Messina, c/o Dipartimento di Scienze del Farmaco e dei Prodotti per la Salute (S.C.I.F.A.R.), University of Messina, Viale Annunziata, 98168 Messina, Italy; Centro Integrato di Ricerca (C.I.R.), University Campus Bio-Medico of Rome, Via Álvaro del Portillo 21, 00128 Rome, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Dugo
authors.affiliation: Dipartimento di Scienze del Farmaco e dei Prodotti per la Salute (S.C.I.F.A.R.), University of Messina, Viale Annunziata, 98168 Messina, Italy; Chromaleont s.r.l. A start-up of the University of Messina, c/o Dipartimento di Scienze del Farmaco e dei Prodotti per la Salute (S.C.I.F.A.R.), University of Messina, Viale Annunziata, 98168 Messina, Italy; Centro Integrato di Ricerca (C.I.R.), University Campus Bio-Medico of Rome, Via Álvaro del Portillo 21, 00128 Rome, Italy. Electronic address: lmondello@unime.it.
authors.forename: Luigi
authors.initials: L
authors.lastname: Mondello
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005227:Fatty Acids / Q000032:analysis; D006041:Goats; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D014280:Triglycerides / Q000032:analysis*
chemical_list: D005227:Fatty Acids; D014280:Triglycerides
keywords: Fused-core; Mass spectrometry; Milk; Serially-coupled columns; Triacylglycerols