title: Comparative proteomics of milk fat globule membrane proteins from transgenic cloned cattle.
abstract: The use of transgenic livestock is providing new methods for obtaining pharmaceutically useful proteins. However, the protein expression profiles of the transgenic animals, including expression of milk fat globule membrane (MFGM) proteins, have not been well characterized. In this study, we compared the MFGM protein expression profile of the colostrum and mature milk from three lines of transgenic cloned (TC) cattle, i.e., expressing recombinant human α-lactalbumin (TC-LA), lactoferrin (TC-LF) or lysozyme (TC-LZ) in the mammary gland, with those from cloned non-transgenic (C) and conventionally bred normal animals (N). We identified 1, 225 proteins in milk MFGM, 166 of which were specifically expressed only in the TC-LA group, 265 only in the TC-LF group, and 184 only in the TC-LZ group. There were 43 proteins expressed only in the transgenic cloned animals, but the concentrations of these proteins were below the detection limit of silver staining. Functional analysis also showed that the 43 proteins had no obvious influence on the bovine mammary gland. Quantitative comparison revealed that MFGM proteins were up- or down-regulated more than twofold in the TC and C groups compared to N group: 126 in colostrum and 77 in mature milk of the TC-LA group; 157 in colostrum and 222 in mature milk of the TC-LF group; 49 in colostrum and 98 in mature milk of the TC-LZ group; 98 in colostrum and 132 in mature milk in the C group. These up- and down-regulated proteins in the transgenic animals were not associated with a particular biological function or pathway, which appears that expression of certain exogenous proteins has no general deleterious effects on the cattle mammary gland.
authors.affiliation: State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, PR China.
authors.forename: Shunchao
authors.initials: S
authors.lastname: Sui
authors.affiliation: State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Beijing GenProtein Biotech Company Ltd., Beijing, PR China; Wuxi Kingenew Biotechnology Co., Ltd., Wuxi, Jiangsu Province, China.
authors.forename: Jianwu
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, PR China.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, PR China.
authors.forename: Chengdong
authors.initials: C
authors.lastname: Guo
authors.affiliation: Wuxi Kingenew Biotechnology Co., Ltd., Wuxi, Jiangsu Province, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D002417:Cattle / Q000235:genetics* / Q000378:metabolism; D019976:Cloning, Organism; D003126:Colostrum / Q000737:chemistry / Q000378:metabolism; D005260:Female; D006017:Glycolipids / Q000032:analysis* / Q000235:genetics* / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis* / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007768:Lactalbumin / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D007781:Lactoferrin / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D066292:Lipid Droplets; D008892:Milk / Q000737:chemistry / Q000378:metabolism; D008894:Milk Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D009113:Muramidase / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy; D020543:Proteome / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D006017:Glycolipids; D006023:Glycoproteins; D008894:Milk Proteins; D020543:Proteome; D011994:Recombinant Proteins; C110367:milk fat globule; D007768:Lactalbumin; D009113:Muramidase; D007781:Lactoferrin