title: Sorghum [Sorghum bicolor (L.) Moench] leaf sheath dye protects against cisplatin-induced hepatotoxicity and oxidative stress in rats.
abstract: This study sought to determine the protective effect of dietary inclusion of sorghum leaf sheath dye on cisplatin-induced hepatotoxicity and oxidative stress in rats. Adult male rats were randomly divided into four groups with six animals in each group. Groups I and II were fed a basal diet, while groups III and IV were fed diets containing 0.5% and 1% sorghum leaf sheath dye, respectively, for 20 days before cisplatin administration. Hepatotoxicity was induced by a single dose of cisplatin (7 mg/kg body weight, i.p.), and the experiment was terminated at 3 days after cisplatin injection. The liver and plasma were studied for hepatotoxicity and antioxidant capacity. Cisplatin caused a significant (P<.05) alteration in plasma and liver enzymatic (catalase, glutathione-S-transferase [GST], and superoxide dismutase [SOD]) and nonenzymatic (glutathione [GSH] and vitamin C) antioxidant indices with a concomitant increase in the malondialdehyde (MDA) content; however, there was a significant (P<.05) restoration of the antioxidant status coupled with a significant (P<.05) decrease in the tissue MDA content, after consumption of diets containing sorghum leaf sheath dye. Furthermore, dietary inclusion of sorghum leaf sheath dye caused a marked reduction in the activities of alanine aminotransferase and aspartate aminotransferase after cisplatin administration. However, the ability of the dye to prevent significant cisplatin-induced alteration of both plasma and liver antioxidant indices suggests an antioxidant mechanism of action. Hence, this protective effect of Sorghum bicolor leaf sheath dye against cisplatin-induced hepatotoxicity in rats reflects its potential and beneficial role in the prevention of liver damage associated with cisplatin administration.
authors.affiliation: 1 Functional Foods, Nutraceuticals and Phytomedicine Unit, Department of Biochemistry, Federal University of Technology , Akure, Nigeria .
authors.forename: Adedayo O
authors.initials: AO
authors.lastname: Ademiluyi
authors.forename: Ganiyu
authors.initials: G
authors.lastname: Oboh
authors.forename: Oluwaseun J
authors.initials: OJ
authors.lastname: Agbebi
authors.forename: Aline A
authors.initials: AA
authors.lastname: Boligon
authors.forename: Margareth L
authors.initials: ML
authors.lastname: Athayde
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001219:Aspartate Aminotransferases / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism; D002945:Cisplatin / Q000633:toxicity; D008099:Liver / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D020011:Protective Agents / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D045868:Sorghum / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D020011:Protective Agents; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D002945:Cisplatin
keywords: Sorghum bicolor (L.) Moench; anthocyanin; antioxidants; cis-dichlorodiammineplatinum (II) (CDDP); liver damage; oxidative stress; red dye