title: [Association of polymorphisms of HLA-DRB1 gene with unexplained recurrent spontaneous abortion in ethnic Hans from Henan].
abstract: OBJECTIVE
  To assess the association of polymorphisms of human leukocyte antigen DRB1 gene (HLA-DRB1) with susceptibility to unexplained recurrent spontaneous abortion (URSA).
  
  
  METHODS
  The HLA-DRB1 gene was typed with polymerase chain reaction-specific sequence primers (PCR-SSP) method in 200 couples with URSA and 200 couples with a normal pregnancy history.
  
  
  RESULTS
  The frequencies of DRB1*09 and DRB1*13 alleles were significantly greater in the URSA group compared with the control group (14.50% vs. 9.50%, and 7.00% vs. 4.38%, both P<0.05), whilst the frequencies of DRB1*04 and DRB1*12 alleles were significantly lower (7.13% vs. 10.75%, and 8.63% vs. 14.38%, both P<0.05). For females from the URSA group, the frequency of DRB1*09 allele (14.00%) was significantly higher compared with the controls (9.25%) (P=0.036), whilst the frequency of DRB1*12(8.50%) allele was significantly lower (14.00%) (P=0.014). For males in the URSA group, the frequencies of DRB1*09 and DRB1*13 alleles were significantly higher than those of the controls (15.00% vs. 9.75%, and 9.25% vs. 4.00%, both P<0.05), whilst the frequencies of DRB1*04 and DRB1*12 alleles were significantly lower (5.75% vs. 12.25%, and 8.75% vs. 14.75%, P<0.05).
  
  
  CONCLUSIONS
  The DRB1*09 and DRB1*13 alleles may contribute to the susceptibility of URSA, while DRB1*04 and DRB1*12 alleles may confer a protective effect factors. For females, however, no significant association of DRB1*13 and DRB1*04 alleles with URSA was found.
authors.affiliation: People's Hospital of Zhengzhou University, Zhengzhou, Henan 450003, P.R. China. michellehecat@126.com.
authors.forename: Miao
authors.initials: M
authors.lastname: He
authors.forename: Bing
authors.initials: B
authors.lastname: Kang
authors.forename: Shixiu
authors.initials: S
authors.lastname: Liao
authors.forename: Ke
authors.initials: K
authors.lastname: Yang
authors.forename: Xuebing
authors.initials: X
authors.lastname: Ding
authors.forename: Dong
authors.initials: D
authors.lastname: Wu
authors.forename: Qiannan
authors.initials: Q
authors.lastname: Guo
authors.forename: Qiaofang
authors.initials: Q
authors.lastname: Hou
mesh_terms: D000022:Abortion, Spontaneous / Q000208:ethnology / Q000235:genetics*; D000483:Alleles; D044466:Asian People / Q000208:ethnology / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D059811:HLA-DRB1 Chains / Q000235:genetics*; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D055815:Young Adult
chemical_list: D059811:HLA-DRB1 Chains