title: CYP1A2 polymorphism in Chinese patients with acute liver injury induced by Polygonum multiflorum.
abstract: The objective of this study was to evaluate the genotype and allelic frequencies of CYP1A2 in Chinese patients with acute liver injury induced by Polygonum multiflorum. We examined the clinical mechanism of acute liver injury induced by P. multiflorum. According to the diagnostic criteria for drug-induced liver injury (DILI), 43 cases of P. multiflorum-induced liver injury admitted to the First Affiliated Hospital, Zhejiang University were identified between January 2008 and December 2012. An additional 43 control subjects were also chosen. Several alleles, including 1C, 1F, 2, 7, 9, and 11 of CYP1A2 were amplified from genomic DNA and sequenced. We used the chi-square test to determine whether CYP1A2 allele polymorphisms are associated with acute liver injury induced by P. multiflorum. The frequency of the CYP1A2 1C allele was 46.5% in P. multiflorum-induced DILI patients, which was significantly different from the frequency of 27.9% observed in healthy subjects. The frequency of the CYP1A2 1F allele was 63.9% in P. multiflorum-induced DILI patients, compared to 57.0% in healthy controls; the difference was not significant. The allelic frequencies of CYP1A2 2, CYP1A2 7, CYP1A2 9, and CYP1A2 11 were too low to be detected. The frequency of the CYP1A2 1C mutation in Chinese patients with P. multiflorum-induced acute liver injury differed from that in healthy Chinese people, indicating that CYP1A2 1C is probably related to metabolism of P. multiflorum, which is followed by acute liver injury.
authors.affiliation: The First Affiliated Hospital, Zhejiang University, Hangzhou, China.
authors.forename: K F
authors.initials: KF
authors.lastname: Ma
authors.affiliation: The First Affiliated Hospital, Zhejiang University, Hangzhou, China.
authors.forename: X G
authors.initials: XG
authors.lastname: Zhang
authors.affiliation: The First Affiliated Hospital, Zhejiang University, Hangzhou, China jancemkf@163.com.
authors.forename: H Y
authors.initials: HY
authors.lastname: Jia
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People; D016022:Case-Control Studies; D056486:Chemical and Drug Induced Liver Injury / Q000235:genetics*; D002681:China; D019388:Cytochrome P-450 CYP1A2 / Q000235:genetics*; D004365:Drugs, Chinese Herbal / Q000009:adverse effects; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D028502:Polygonum / Q000009:adverse effects / Q000737:chemistry; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide
chemical_list: D004365:Drugs, Chinese Herbal; D019388:Cytochrome P-450 CYP1A2