title: A Fasciola hepatica-derived fatty acid binding protein induces protection against schistosomiasis caused by Schistosoma bovis using the adjuvant adaptation (ADAD) vaccination system.
abstract: Several efforts have been made to identify anti-schistosomiasis vaccine candidates and new vaccination systems. The fatty acid binding protein (FAPB) has been shown to induce a high level of protection in trematode infection. The adjuvant adaptation (ADAD) vaccination system was used in this study, including recombinant FABP, a natural immunomodulator and saponins. Mice immunised with the ADAD system were able to up-regulate proinflammatory cytokines (IL-1 and IL-6) and induce high IgG2a levels. Moreover, there was a significant reduction in worm burden, egg liver and hepatic lesion in vaccinated mice in two independent experiments involving Schistosoma bovis infected mice. The foregoing data shows that ADAD system using FABP provide a good alternative for triggering an effective immune response against animal schistosomiasis.
authors.affiliation: Laboratorio de Inmunología y Parasitología Molecular, Centro de Investigación de Enfermedades Tropicales de la, Universidad de Salamanca (IBSAL-CIETUS), Avda Licenciado Méndez Nieto s/n, 37007 Salamanca, Spain.
authors.forename: Belén
authors.initials: B
authors.lastname: Vicente
authors.affiliation: Laboratorio de Inmunología y Parasitología Molecular, Centro de Investigación de Enfermedades Tropicales de la, Universidad de Salamanca (IBSAL-CIETUS), Avda Licenciado Méndez Nieto s/n, 37007 Salamanca, Spain. Electronic address: jlaban@usal.es.
authors.forename: Julio
authors.initials: J
authors.lastname: López-Abán
authors.affiliation: Laboratorio de Inmunología y Parasitología Molecular, Centro de Investigación de Enfermedades Tropicales de la, Universidad de Salamanca (IBSAL-CIETUS), Avda Licenciado Méndez Nieto s/n, 37007 Salamanca, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Rojas-Caraballo
authors.affiliation: Laboratorio de Inmunología y Parasitología Molecular, Centro de Investigación de Enfermedades Tropicales de la, Universidad de Salamanca (IBSAL-CIETUS), Avda Licenciado Méndez Nieto s/n, 37007 Salamanca, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Pérez del Villar
authors.affiliation: Department of Pathology and Laboratory Medicine (Suite 617-A), University of Puerto Rico School of Medicine, P.O. Box 365067, San Juan, PR 00936-5067, Puerto Rico.
authors.forename: George V
authors.initials: GV
authors.lastname: Hillyer
authors.affiliation: Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense, Pza. Ramón y Cajal s/n, 28040 Madrid, Spain.
authors.forename: Antonio R
authors.initials: AR
authors.lastname: Martínez-Fernández
authors.affiliation: Laboratorio de Inmunología y Parasitología Molecular, Centro de Investigación de Enfermedades Tropicales de la, Universidad de Salamanca (IBSAL-CIETUS), Avda Licenciado Méndez Nieto s/n, 37007 Salamanca, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Muro
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry; D000818:Animals; D016207:Cytokines / Q000378:metabolism; D005210:Fasciola hepatica / Q000737:chemistry / Q000276:immunology*; D050556:Fatty Acid-Binding Proteins / Q000276:immunology*; D005260:Female; D015801:Helminth Proteins / Q000737:chemistry / Q000276:immunology*; D007279:Injections, Subcutaneous; D051379:Mice; D008807:Mice, Inbred BALB C; D062605:Quillaja Saponins / Q000276:immunology; D011897:Random Allocation; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D012547:Schistosoma / Q000276:immunology; D012552:Schistosomiasis / Q000469:parasitology / Q000517:prevention & control*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014611:Vaccination / Q000379:methods; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D050556:Fatty Acid-Binding Proteins; D015801:Helminth Proteins; D062605:Quillaja Saponins; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic
keywords: Adjuvant; Fatty acid-binding protein; Schistosoma bovis; Vaccine; rFh15