title: Knockdown of Aurora-B inhibits osteosarcoma cell invasion and migration via modulating PI3K/Akt/NF-κB signaling pathway.
abstract: Increasing evidences reveal that Aurora-B may be involved in metastasis of malignant tumor. In this study, we investigated the inhibitory effect of Aurora-B on invasion and migration of OS cells and the activity of PI3K/Akt/NF-κB signaling pathway in vitro. The expression of Aurora-B and p-Akt (Ser473) proteins was detected by immunohistochemistry in OS tissues from 24 patients with pulmonary metastatic disease, and the relationship between Aurora-B and p-Akt was investigated. The results showed that there was a positive correlation between Aurora-B and p-Akt protein expression. Furthermore, we down-regulated the expression of Aurora-B through a recombinant lentivirus (Lv-shAURKB). Migration and invasion of cells were investigated by wound healing and transwell invasion assays. Results showed that silencing Aurora-B inhibited cell migratory and invasive ability of OS cells in vitro. Finally, knockdown of Aurora-B suppresses the activity of PI3K/Akt/NF-κB signaling pathway in OS cells. Our results indicated that knockdown of Aurora-B suppresses OS cells migratory and invasive ability via modulating the "PI3K/Akt/NF-κB" signaling pathway in vitro. The Aurora-B blocker may be a new therapeutic strategy in OS management.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Nanchang University Jiangxi, China.
authors.forename: Liang Bo
authors.initials: LB
authors.lastname: Zhu
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Nanchang University Jiangxi, China ; Department of Orthopedics, People's Hospital of Shangrao City Jiangxi, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Anesthesia, The First Affiliated Hospital of Nanchang University Jiangxi, China.
authors.forename: Xiao Ping
authors.initials: XP
authors.lastname: Zhu
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Nanchang University Jiangxi, China.
authors.forename: Tao Fang
authors.initials: TF
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Nanchang University Jiangxi, China.
authors.forename: Xuan Yin
authors.initials: XY
authors.lastname: Chen
authors.affiliation: Department of Pathology, Cancer Hospital of Jiangxi Province Jiangxi, China.
authors.forename: Qin Feng
authors.initials: QF
authors.lastname: Luo
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Nanchang University Jiangxi, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Shu
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Nanchang University Jiangxi, China.
authors.forename: Zhi Li
authors.initials: ZL
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Nanchang University Jiangxi, China.
authors.forename: Shan Hu
authors.initials: SH
authors.lastname: Huang
mesh_terms: D064107:Aurora Kinase B / Q000378:metabolism*; D015153:Blotting, Western; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D016328:NF-kappa B / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000473:pathology; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology
chemical_list: D016328:NF-kappa B; D019869:Phosphatidylinositol 3-Kinases; C576921:AURKB protein, human; D064107:Aurora Kinase B; D051057:Proto-Oncogene Proteins c-akt
keywords: Aurora-B; Osteosarcoma; PI3K/Akt/NF-κB signaling pathway; invasion and migration