title: Expression of serum survivin protein in diagnosis and prognosis of gallbladder cancer: a comparative study.
abstract: The role of survivin in gallbladder cancer (GBC) has not been evaluated. We investigated survivin protein expression in serum of patients with gallbladder diseases (cholelithiasis, n = 30; GBC, n = 39) and compared with healthy controls (n = 25). Clinicopathological parameters, diagnosis and prognosis of patients with GBC were correlated with the expression of serum survivin by enzyme-linked immunosorbent assay. Significantly higher (P < 0.0001) expression of survivin protein was observed in GBC as compared to cholelithiasis and control. Increased survivin expression was significantly associated with higher tumor stage (stage III vs. stage II; P < 0.0001) and cellular differentiation (poor and moderate vs. well differentiated; P < 0.0001) in GBC. No significant correlation was observed with any of the other clinico-pathological parameters studied. The cutoff value of survivin protein of 79 pg/ml with sensitivity of 81.16 % and specificity of 80 % differentiated the diseased group (cholelithiasis or GBC) from control group were as the cutoff value of 109 pg/ml differentiated GBC from cholelithiasis with a sensitivity of 82.05 % and specificity of 93.33 %. Though not significant, increased expression of survivin was associated with median overall survival (12 vs. 18 months; P = 0.05) in GBC patients. Our study suggests that survivin protein in serum could be both a useful diagnostic marker and an important prognostic factor for GBC.
authors.affiliation: Department of Surgical Gastroenterology, King George's Medical University, Lucknow, 226003, Uttar Pradesh, India.
authors.forename: Jaya
authors.initials: J
authors.lastname: Nigam
authors.forename: Abhijit
authors.initials: A
authors.lastname: Chandra
authors.forename: Hasan Raza
authors.initials: HR
authors.lastname: Kazmi
authors.forename: Anshuman
authors.initials: A
authors.lastname: Singh
authors.forename: Vishal
authors.initials: V
authors.lastname: Gupta
authors.forename: Devendra
authors.initials: D
authors.lastname: Parmar
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Srivastava
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood; D016022:Case-Control Studies; D002769:Cholelithiasis; D005260:Female; D005706:Gallbladder Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology* / Q000401:mortality; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000097:blood*; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012372:ROC Curve; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D014408:Biomarkers, Tumor; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin