title: Pretreatment with mechano-growth factor E peptide protects bone marrow mesenchymal cells against damage by fluid shear stress.
abstract: Improper fluid shear stress (FSS) can cause serious damages to bone marrow mesenchymal stem cells (MSCs). Mechano-growth factor (MGF) E peptide pretreatment was proposed to protect MSCs against FSS damage in this study. MSCs were exposed to FSS for 30 min after they were pretreated with MGF E peptide for 24 h. Then, the effects of MGF E peptide on the viability, proliferation and cell apoptosis of MSCs were investigated. MGF E peptide pretreatment could recover the cellular metabolic activity of MSCs reduced by 72 dyne cm(-2) FSS and had a synergistic effect with FSS on the cellular metabolic viability of MSCs under 24 and 72 dyne cm(-2) FSS. These results suggested that MGF E peptide pretreatment could be an effective method for the protection of FSS damage in bone tissue engineering.
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, 400044, P. R., China, yglv@cqu.edu.cn.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Lv
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Hao
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Sha
authors.forename: Li
authors.initials: L
authors.lastname: Yang
mesh_terms: D017209:Apoptosis; D001696:Biomechanical Phenomena; D001853:Bone Marrow / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000502:physiology*
chemical_list: C523997:mechano-growth factor E, human; D007334:Insulin-Like Growth Factor I