title: The ABC transporter ABCG1 is required for suberin formation in potato tuber periderm.
abstract: The lipid biopolymer suberin plays a major role as a barrier both at plant-environment interfaces and in internal tissues, restricting water and nutrient transport. In potato (Solanum tuberosum), tuber integrity is dependent on suberized periderm. Using microarray analyses, we identified ABCG1, encoding an ABC transporter, as a gene responsive to the pathogen-associated molecular pattern Pep-13. Further analyses revealed that ABCG1 is expressed in roots and tuber periderm, as well as in wounded leaves. Transgenic ABCG1-RNAi potato plants with downregulated expression of ABCG1 display major alterations in both root and tuber morphology, whereas the aerial part of the ABCG1-RNAi plants appear normal. The tuber periderm and root exodermis show reduced suberin staining and disorganized cell layers. Metabolite analyses revealed reduction of esterified suberin components and hyperaccumulation of putative suberin precursors in the tuber periderm of RNA interference plants, suggesting that ABCG1 is required for the export of suberin components.
authors.affiliation: Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, D-06120 Halle (Saale), Germany.
authors.forename: Ramona
authors.initials: R
authors.lastname: Landgraf
authors.affiliation: Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, D-06120 Halle (Saale), Germany.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Smolka
authors.affiliation: Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, D-06120 Halle (Saale), Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Altmann
authors.affiliation: Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, D-06120 Halle (Saale), Germany.
authors.forename: Lennart
authors.initials: L
authors.lastname: Eschen-Lippold
authors.affiliation: Friedrich Alexander University Erlangen-Nürnberg, Department of Biology, D-91058 Erlangen, Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Senning
authors.affiliation: Friedrich Alexander University Erlangen-Nürnberg, Department of Biology, D-91058 Erlangen, Germany.
authors.forename: Sophia
authors.initials: S
authors.lastname: Sonnewald
authors.affiliation: Leibniz Institute of Plant Biochemistry, Department of Bioorganic Chemistry, D-06120 Halle (Saale), Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Weigel
authors.affiliation: Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, D-06120 Halle (Saale), Germany.
authors.forename: Nadezhda
authors.initials: N
authors.lastname: Frolova
authors.affiliation: Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, D-06120 Halle (Saale), Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Strehmel
authors.affiliation: Martin Luther University Halle-Wittenberg, Biocenter, D-06120 Halle (Saale), Germany.
authors.forename: Gerd
authors.initials: G
authors.lastname: Hause
authors.affiliation: Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, D-06120 Halle (Saale), Germany.
authors.forename: Dierk
authors.initials: D
authors.lastname: Scheel
authors.affiliation: Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, D-06120 Halle (Saale), Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Böttcher
authors.affiliation: Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, D-06120 Halle (Saale), Germany srosahl@ipb-halle.de.
authors.forename: Sabine
authors.initials: S
authors.lastname: Rosahl
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000032:analysis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001692:Biological Transport / Q000235:genetics; D002462:Cell Membrane / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008055:Lipids / Q000096:biosynthesis*; D020411:Oligonucleotide Array Sequence Analysis; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000378:metabolism; D034622:RNA Interference; D011198:Solanum tuberosum / Q000235:genetics / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D008055:Lipids; D010940:Plant Proteins; C065875:suberin