title: Histology validation of mapping depth-resolved cardiac fiber orientation in fresh mouse heart using optical polarization tractography.
abstract: Myofiber organization in cardiac muscle plays an important role in achieving normal mechanical and electrical heart functions. An imaging tool that can reveal microstructural details of myofiber organization is valuable for both basic research and clinical applications. A high-resolution optical polarization tractography (OPT) was recently developed based on Jones matrix optical coherence tomography (JMOCT). In this study, we validated the accuracy of using OPT for measuring depth-resolved fiber orientation in fresh heart samples by comparing directly with histology images. Systematic image processing algorithms were developed to register OPT with histology images. The pixel-wise differences between the two tractographic results were analyzed in details. The results indicate that OPT can accurately image depth-resolved fiber orientation in fresh heart tissues and reveal microstructural details at the histological level.
authors.affiliation: Department of Bioengineering, University of Missouri, Columbia, MO 65211 USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Molecular Microbiology & Immunology, University of Missouri, Columbia, MO 65211 USA.
authors.forename: K
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Department of Molecular Microbiology & Immunology, University of Missouri, Columbia, MO 65211 USA.
authors.forename: N B
authors.initials: NB
authors.lastname: Wasala
authors.affiliation: Department of Bioengineering, University of Missouri, Columbia, MO 65211 USA.
authors.forename: X
authors.initials: X
authors.lastname: Yao
authors.affiliation: Department of Molecular Microbiology & Immunology, University of Missouri, Columbia, MO 65211 USA.
authors.forename: D
authors.initials: D
authors.lastname: Duan
authors.affiliation: Department of Bioengineering, University of Missouri, Columbia, MO 65211 USA.
authors.forename: G
authors.initials: G
authors.lastname: Yao
keywords: (110.4500) Optical coherence tomography; (170.6935) Tissue characterization; (230.5440) Polarization-selective devices