title: The effect of ph on the fertilizability of rainbow trout (Oncorhynchus mykiss) eggs stored in a chilled state.
abstract: To verify the observation that fertilizability of stored rainbow trout eggs is affected by deviations in pH (Komrakova and Holtz, 2011) freshly collected eggs were immersed in coelomic fluid adjusted to pH 6, 7, 8, 9, or 10 (3 replicates each) and stored at 2 degreesC. At 0, 1, 24, 48, and 96h, pH was measured and eggs were inseminated with cryopreserved semen. Egg development until the eyed stage proceeded normally in all cases. However hatching rates, amounting to 70% in controls (pH 8, SEM 0.02), were reduced to 50% in samples exposed to pH 7 or pH 9 and to 40% and 30% in samples exposed to pH 6 and pH 10, respectively. In conclusion, when eggs are subjected to coelomic fluid with deviating pH, the damage afflicted upon the eggs will not become evident until they are due to hatch.
authors.forename: M
authors.initials: M
authors.lastname: Komrakova
authors.forename: U
authors.initials: U
authors.lastname: Kuembet
authors.forename: W
authors.initials: W
authors.lastname: Holtz
mesh_terms: D000818:Animals; D005306:Fertilization; D005398:Fisheries / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D017686:Oncorhynchus mykiss / Q000502:physiology*; D010063:Ovum / Q000502:physiology*; D012034:Refrigeration; D014867:Water / Q000737:chemistry*
chemical_list: D014867:Water