title: Effect of Fibulin-5 on cell proliferation and invasion in human gastric cancer patients.
abstract: OBJECTIVE
  To explore the effect of Fibulin-5 expression on cell proliferation and invasion in human gastric cancer patients.
  
  
  METHODS
  Fibulin-5 expression was detected in 56 samples of surgically resected gastric cancer and paired noncancerous tissues using qRT-PCR and immunoblotting. Fibulin-5 was knocked down by Fibulin-5 shRNA in MGC-803 cells, then BrdU cell proliferation and transwell invasion assays were used to determine cell proliferation and invasion.
  
  
  RESULTS
  The level of Fibulin-5 mRNA in gastric cancer tissues was significantly higher as compared with that in normal tumor-adjacent tissues (P<0.05). Otherwise, the level of Fibulin-5 protein in cancer and noncancerous tissues was consistent with mRNA expression (P<0.05). Fibulin-5 protein expression in tumor tissues with poorly differentiated, lymph node metastasis and advanced TNM tumor stage was significantly higher (P<0.05, respectively). Fibulin-5 was obviously knocked down by Fibulin-5 shRNA (P<0.05), and Fibulin-5 knockdown significantly inhibited cell proliferation and invasion in MGC-803 cells (P<0.05, respectively).
  
  
  CONCLUSIONS
  The high-expression of Fibulin-5 is associated with the malignant clinicopathologic parameters in gastric cancer and Fibulin-5 knockdown inhibits cell proliferation and invasion in MGC-803 cells, suggesting Fibulin-5 may act as a key factor in the progression of gastric cancer.
authors.affiliation: Department of General Surgery, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Shi
authors.affiliation: Department of General Surgery, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of General Surgery, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, China.
authors.forename: Chun-Hui
authors.initials: CH
authors.lastname: Cao
authors.affiliation: Department of General Surgery, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, China.
authors.forename: Zhi-Yu
authors.initials: ZY
authors.lastname: Li
authors.affiliation: Department of General Surgery, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of General Surgery, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, China. Electronic address: chenli335598@163.com.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
keywords: Clinical significance; Fibulin-5; Gastric cancer; Invasion; Proliferation