title: Platelet-derived growth factor mediates interleukin-13-induced collagen I production in mouse airway fibroblasts.
abstract: Interleukin-13 (IL-13) is associated with the production of collagen in airway remodelling of asthma. Yet, the molecular mechanisms underlying IL-13 induction of collagen remain unclear; the aim of this study is to address this issue. IL-13 dose- and time-dependently-induced collagen I production in primary cultured airway fibroblasts; this was accompanied with the STAT6 phosphorylation, and pre-treatment of cells with JAK inhibitor suppressed IL-13- induced collagen I production. Further study indicated that IL-13 stimulated JAK/STAT6-dependent PDGF production and subsequent ERK1/2 MAPK activation in airway fibroblasts, and the presence of either PDGF receptor blocker or MEK inhibitor partially suppressed IL-13-induced collagen I production. Taken together, our study suggests that activation of JAK/STAT6 signal pathway and subsequent PDGF generation and resultant ERK1/2 MAPK activation mediated IL-13-induced collagen I production in airway fibroblasts.
authors.affiliation: Respiratory Diseases Research Center, and Department of Respiratory Medicine, The Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, Shaanxi, 710004, P.R. China.
authors.forename: Jiamei
authors.initials: J
authors.lastname: Lu
authors.forename: Yanting
authors.initials: Y
authors.lastname: Zhu
authors.forename: Wei
authors.initials: W
authors.lastname: Feng
authors.forename: Yilin
authors.initials: Y
authors.lastname: Pan
authors.forename: Shaojun
authors.initials: S
authors.lastname: Li
authors.forename: Dong
authors.initials: D
authors.lastname: Han
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.forename: Xinming
authors.initials: X
authors.lastname: Xie
authors.forename: Guizuo
authors.initials: G
authors.lastname: Wang
authors.forename: Manxiang
authors.initials: M
authors.lastname: Li
mesh_terms: D056151:Airway Remodeling / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism; D015151:Immunoblotting; D018793:Interleukin-13 / Q000378:metabolism*; D053612:Janus Kinases / Q000378:metabolism; D051379:Mice; D010766:Phosphorylation; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D050800:STAT6 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
chemical_list: D024042:Collagen Type I; D018793:Interleukin-13; D010982:Platelet-Derived Growth Factor; D050800:STAT6 Transcription Factor; D053612:Janus Kinases