title: [Role of new K(+) channel blocker 4-AP-3-MeOH in acute spinal cord compression injury in rats].
abstract: OBJECTIVE
  To evaluate the effects of 4-aminopyridine-3-methyl hydroxide (4-AP-3-MeOH) in rat's acute spinal cord injury.
  
  
  METHODS
  A total of 12 adult male SD rats (250-300 g) were randomly divided into treatment (n = 6) and control (n = 6) groups. After compressing segment T11 of spinal cord for 30 min, the injured segment received 1 ml 4-AP-3-MeOH (100 µmol/ml) by topically application in treatment group while the control group received 1 ml saline.Somatosensory evoked potential (SSEP) was detected in both groups at pre-injury, 30 min post-injury and post-dosing. Then Luxol fast blue (LFB) staining of target spinal segment was performed.
  
  
  RESULTS
  In treatment group, the values of SSEP at pre-injury, 30 min post-injury and post-dosing were 1.26 ± 0.35, 0.03 ± 0.05 and 0.45 ± 0.19 µv respectively. Comparing SSEP of 30 min post-injury with post-dosing, the difference was statistically significant (P < 0.01).While in control group, the values of SSEP at pre-injury, 30 min post-injury and post-dosing were 1.05 ± 0.39, 0.01 ± 0.02 and 0.02 ± 0.02 µv respectively. Comparing SSEP of 30 min post-injury with post-dosing, there was no statistical difference (P > 0.05). After 30 min injury, there were swelling and bleeding of spinal cord.LFB staining showed that both gray and white matter had swelling and bleeding and central canal was destroyed with varying degrees of demyelination.
  
  
  CONCLUSIONS
  After 30 min of acute spinal cord injury, there are bleeding of gray and white matter with varying degrees of demyelination. Topical usage of K(+) blocker 4-AP-3-MeOH can effectively improve the conduction of SSEP after acute spinal cord injury in rats.
authors.affiliation: Department of Orthopedics, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Institute of Trauma & Orthopedics, Shanghai 200025, China.
authors.forename: Yuehuan
authors.initials: Y
authors.lastname: Zheng
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zou
authors.forename: Zhe
authors.initials: Z
authors.lastname: Chen
authors.forename: Zezhu
authors.initials: Z
authors.lastname: Zhou
authors.forename: Yingying
authors.initials: Y
authors.lastname: Shi
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Wang
authors.affiliation: Email: caopeng8@yahoo.com.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Cao
mesh_terms: D000631:Aminopyridines / Q000627:therapeutic use*; D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D009431:Neural Conduction / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000503:physiopathology; D013117:Spinal Cord Compression / Q000188:drug therapy* / Q000503:physiopathology
chemical_list: C548134:4-aminopyridine-3-methanol; D000631:Aminopyridines