title: Bioevaluation of novel anti-biofilm coatings based on PVP/Fe3O4 nanostructures and 2-((4-ethylphenoxy)methyl)-N- (arylcarbamothioyl)benzamides.
abstract: Novel derivatives were prepared by reaction of aromatic amines with 2-(4-ethylphenoxymethyl)benzoyl isothiocyanate, affording the N-[2-(4-ethylphenoxymethyl) benzoyl]-Nꞌ-(substituted phenyl)thiourea. Structural elucidation of these compounds was performed by IR, NMR spectroscopy and elemental analysis. The new compounds were used in combination with Fe3O4 and polyvinylpyrrolidone (PVP) for the coating of medical surfaces. In our experiments, catheter pieces were coated by Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique. The microbial adherence ability was investigated in 6 multi-well plates by using culture based methods. The obtained surfaces were also assessed for their cytotoxicity with respect to osteoblast cells, by using fluorescence microscopy and MTT assay. The prepared surfaces by advanced laser processing inhibited the adherence and biofilm development ability of Staphylococcus aureus and Pseudomonas aeruginosa tested strains while cytotoxic effects on the 3T3-E1 preosteoblasts embedded in layer shaped alginate hydrogels were not observed. These results suggest that the obtained medical surfaces, based on the novel thiourea derivatives and magnetic nanoparticles with a polymeric shell could represent a promising alternative for the development of new and effective anti-infective strategies.
authors.affiliation: Department of Pharmaceutical Chemistry, "Carol Davila" University of Medicine and Pharmacy, Traian Vuia No. 6, 020956 Bucharest, Romania. carmen_limban@yahoo.com.
authors.forename: Carmen
authors.initials: C
authors.lastname: Limban
authors.affiliation: Department of Pharmaceutical Chemistry, "Carol Davila" University of Medicine and Pharmacy, Traian Vuia No. 6, 020956 Bucharest, Romania. missir_alexandru@yahoo.com.
authors.forename: Alexandru Vasile
authors.initials: AV
authors.lastname: Missir
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1-7,011061 Bucharest, Romania. grumezescu@yahoo.com.
authors.forename: Alexandru Mihai
authors.initials: AM
authors.lastname: Grumezescu
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1-7,011061 Bucharest, Romania. elena_oprea_93@yahoo.co.uk.
authors.forename: Alexandra Elena
authors.initials: AE
authors.lastname: Oprea
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1-7,011061 Bucharest, Romania. valentina.grumezescu@inflpr.ro.
authors.forename: Valentina
authors.initials: V
authors.lastname: Grumezescu
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1-7,011061 Bucharest, Romania. bogdan.vasile@upb.ro.
authors.forename: Bogdan Stefan
authors.initials: BS
authors.lastname: Vasile
authors.affiliation: National Institute for Lasers, Plasma & Radiation Physics, Lasers Department, P.O. Box MG-36, Bucharest-Magurele, Romania. gabriel.socol@inflpr.ro.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Socol
authors.affiliation: Metav-CD S.A., 31Rosetti Str., 020015 Bucharest, Romania. roxanatrusca@yahoo.com.
authors.forename: Roxana
authors.initials: R
authors.lastname: Trușcă
authors.affiliation: The Organic Chemistry Center of Romanian Academy "Costin C.D. Nenitescu" Bucharest, Splaiul Independentei, 202B, 77208 Bucharest, Romania. mtc@cco.ro.
authors.forename: Miron Teodor
authors.initials: MT
authors.lastname: Caproiu
authors.affiliation: Department of Microbiology, Faculty of Biology, University of Bucharest, Research Institute ofUniversity of Bucharest, Aleea Portocalelor No. 1-3, 060101 Bucharest, Romania. carmen_balotescu@yahoo.com.
authors.forename: Mariana Carmen
authors.initials: MC
authors.lastname: Chifiriuc
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independenței, 050095 Bucharest, Romania. bianca.galateanu@gmail.com.
authors.forename: Bianca
authors.initials: B
authors.lastname: Gălățeanu
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independenței, 050095 Bucharest, Romania. marietacostache@yahoo.com.
authors.forename: Marieta
authors.initials: M
authors.lastname: Costache
authors.affiliation: Department of Pharmaceutical Chemistry, "Carol Davila" University of Medicine and Pharmacy, Traian Vuia No. 6, 020956 Bucharest, Romania. morusciag@yahoo.com.
authors.forename: Laurențiu
authors.initials: L
authors.lastname: Morușciag
authors.affiliation: Conway Institute, University College Dublin, Dublin 4, Dublin, Ireland . carmen_limban@yahoo.com.
authors.forename: Grațiela
authors.initials: G
authors.lastname: Pîrcălăbioru
authors.affiliation: Department of Pharmaceutical Chemistry, "Carol Davila" University of Medicine and Pharmacy, Traian Vuia No. 6, 020956 Bucharest, Romania. diananuta@yahoo.com.
authors.forename: Diana Camelia
authors.initials: DC
authors.lastname: Nuță
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D001549:Benzamides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D058085:Iron Compounds / Q000737:chemistry* / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D049329:Nanostructures / Q000008:administration & dosage / Q000737:chemistry; D011145:Polyvinyls / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D011759:Pyrrolidines / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D001549:Benzamides; D058085:Iron Compounds; D011145:Polyvinyls; D011759:Pyrrolidines; C505150:poly(N-vinylpyrrolidine)