title: Acute in vivo response to an alternative implant for urogynecology.
abstract: OBJECTIVE
  To investigate in vivo the acute host response to an alternative implant designed for the treatment of stress urinary incontinence (SUI) and pelvic organ prolapse (POP).
  
  
  METHODS
  A biodegradable scaffold was produced from poly-L-lactic acid (PLA) using the electrospinning technique. Human and rat adipose-derived stem cells (ADSCs) were isolated and characterized by fluorescence-activated cell sorting and differentiation assays. PLA scaffolds were seeded and cultured for 2 weeks with human or rat ADSCs. Scaffolds with and without human or rat ADSCs were implanted subcutaneously on the abdominal wall of rats. After 3 and 7 days, 6 animals from each group were sacrificed. Sections from each sample were analyzed by Haematoxylin and Eosin staining, Sirius red staining, and immunohistochemistry for CD68, PECAM-1, and collagen I and III.
  
  
  RESULTS
  Animals responded to the scaffolds with an acute macrophage response. After 7 days of implantation, there was extensive host cell penetration, new blood vessel formation, and new collagen deposition throughout the full thickness of the samples without obvious differences between cell-containing and cell-free scaffolds.
  
  
  CONCLUSIONS
  The acute in vivo response to an alternative implant (both with and without cells) for the treatment of SUI and POP showed good acute integration into the host tissues.
authors.affiliation: Department of Material Science and Engineering, Kroto Research Institute, University of Sheffield, Sheffield S3 7HQ, UK.
authors.forename: Sabiniano
authors.initials: S
authors.lastname: Roman Regueros
authors.affiliation: Department of Development and Regeneration, University Hospital Gasthuisberg, Catholic University of Leuven, 3000 Leuven, Belgium.
authors.forename: Maarten
authors.initials: M
authors.lastname: Albersen
authors.affiliation: Centre for Surgical Technologies, Catholic University of Leuven, 3000 Leuven, Belgium.
authors.forename: Stefano
authors.identifier: 0000-0002-1837-0806
authors.initials: S
authors.lastname: Manodoro
authors.affiliation: Department of Development and Regeneration, University Hospital Gasthuisberg, Catholic University of Leuven, 3000 Leuven, Belgium.
authors.forename: Silvia
authors.initials: S
authors.lastname: Zia
authors.affiliation: Department of Material Science and Engineering, Kroto Research Institute, University of Sheffield, Sheffield S3 7HQ, UK ; Royal Hallamshire Hospital, Sheffield S10 2JF, UK.
authors.forename: Nadir I
authors.initials: NI
authors.lastname: Osman
authors.affiliation: Department of Material Science and Engineering, Kroto Research Institute, University of Sheffield, Sheffield S3 7HQ, UK.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Bullock
authors.affiliation: Royal Hallamshire Hospital, Sheffield S10 2JF, UK.
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Chapple
authors.affiliation: Department of Development and Regeneration, University Hospital Gasthuisberg, Catholic University of Leuven, 3000 Leuven, Belgium ; Centre for Surgical Technologies, Catholic University of Leuven, 3000 Leuven, Belgium.
authors.forename: Jan
authors.identifier: 0000-0002-4920-945X
authors.initials: J
authors.lastname: Deprest
authors.affiliation: Department of Material Science and Engineering, Kroto Research Institute, University of Sheffield, Sheffield S3 7HQ, UK.
authors.forename: Sheila
authors.identifier: 0000-0002-9188-5769
authors.initials: S
authors.lastname: MacNeil
mesh_terms: D020341:Absorbable Implants; D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D003094:Collagen / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000627:therapeutic use*; D008264:Macrophages / Q000187:drug effects / Q000473:pathology; D056887:Pelvic Organ Prolapse / Q000473:pathology / Q000628:therapy*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000627:therapeutic use*; D051381:Rats; D013234:Stem Cells / Q000166:cytology; D054457:Tissue Scaffolds; D014550:Urinary Incontinence, Stress / Q000473:pathology / Q000628:therapy*
chemical_list: D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen