title: Cryo-EM enters a new era.
abstract: Advances in detector hardware and image-processing software have led to a revolution in the use of electron cryo-microscopy to determine complex molecular structures at high resolution.
authors.affiliation: Werner Kuehlbrandt is in the Department of Structural Biology, Max Planck Institute of Biophysics, Frankfurt, Germany werner.kuehlbrandt@biophys.mpg.de.
authors.forename: Werner
authors.initials: W
authors.lastname: Kühlbrandt
mesh_terms: D000465:Algorithms; D053829:Amyloid Precursor Protein Secretases / Q000648:ultrastructure*; D000818:Animals; D020285:Cryoelectron Microscopy / Q000706:statistics & numerical data*; D042967:Electron Transport Complex I / Q000648:ultrastructure*; D006801:Humans; D012270:Ribosomes / Q000648:ultrastructure*; D001616:beta-Galactosidase / Q000648:ultrastructure*
chemical_list: D001616:beta-Galactosidase; D053829:Amyloid Precursor Protein Secretases; D042967:Electron Transport Complex I
keywords: Cryo-EM; image analysis; micoscopy; single-particle analysis