title: [Inhibitory effect of pumpkin protein on expression of Notch signal in RPMI8226 myeloma cells].
abstract: This study was aimed to explore the inhibitory effect of pumpkin protein (cucurmosin, CUS) on proliferation of RPMI8226 myeloma cells in vitro and its mechanism. Western blot was used to detect the expression level of Notch-1, Jagged-2, P-Akt and NF-KB in the myeloma cells treated by different concentrations of CUS. The results demonstrated that CUS could down-regulate the protein expression levels of Notch1, Jagged-2, P-Akt and NF-KB in the myeloma cells and with time-and concentration-dependent way, at the same time CUS could also decrease the expressions of BCL-2 and P-Akt. It is concluded that CUS can obviously inhibit the RPMI8226 cell proliferation in vitro, down-regulate the expression levels of Notch signal and its down-stream target genes. Therefore, Notch signaling pathway can be used as a new treatment target for multiple myeloma, and CUS may be become a potential new drug for regulating Notch signaling pathway.
authors.affiliation: Fujian Institute of Hematology,Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China. E-mail: liutb@medmail.com.cn.
authors.forename: Ting-Bo
authors.initials: TB
authors.lastname: Liu
authors.affiliation: Fujian Provincial College of Medical Sciences, Fuzhou 350001, Fujian Province, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Yang
authors.affiliation: Pharmaceutical College of Fujian Medical University, Fuzhou 350004, Fujian Province, China.
authors.forename: Jie-Ming
authors.initials: JM
authors.lastname: Xie
authors.affiliation: Fujian Institute of Hematology,Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China.
authors.forename: Jian-Da
authors.initials: JD
authors.lastname: Hu
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D000072101:Jagged-2 Protein; D008565:Membrane Proteins / Q000378:metabolism; D009101:Multiple Myeloma / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D010940:Plant Proteins / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051881:Receptor, Notch1 / Q000378:metabolism; D051880:Receptors, Notch / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; C490504:JAG2 protein, human; D000072101:Jagged-2 Protein; D008565:Membrane Proteins; D016328:NF-kappa B; C497761:NOTCH1 protein, human; D010940:Plant Proteins; D051881:Receptor, Notch1; D051880:Receptors, Notch; C412450:cucurmosin protein, Cucurbita moschata; D051057:Proto-Oncogene Proteins c-akt