title: Biocompatibility evaluation of biodentine in subcutaneous tissue of rats.
abstract: BACKGROUND
  Biodentine (Septodont, St-Maur-des-Fossés, France) is a new material suitable for various clinical situations in endodontics, such as perforation repair, retrograde filling, pulp capping, and others. Because it is a new material, its properties should be analyzed before routine clinical use. Thus, this study evaluated the biocompatibility of Biodentine in the subcutaneous tissue of rats.
  
  
  METHODS
  This study was conducted on 15 male rats. Two incisions were made on the dorsal region of each animal for the introduction of 4 tubes. One tube was empty, 1 was filled with zinc oxide-eugenol cement, 1 was filled with mineral trioxide aggregate, and the last tube was filled with Biodentine. After 7, 14, and 30 days, the animals were sacrificed, and the specimens were submitted to histotechnical preparation. The histologic sections were stained with hematoxylin-eosin and analyzed using light microscopy. Scores were established according to the inflammatory process and were statistically compared using the Kruskal-Wallis test (P < .05).
  
  
  RESULTS
  The analysis of the histologic sections evidenced a nonsignificant or mild presence of inflammatory reaction in the connective tissue in contact with the empty tube and the tube containing MTA, which was different from the tube containing zinc oxide eugenol. The connective tissue was moderately inflamed at 7 days when in contact with Biodentine; however, at 14 and 30 days, the inflammatory process was mild or nonsignificant.
  
  
  CONCLUSIONS
  Biodentine was biocompatible with tissue after the 14th day.
authors.affiliation: Discipline of Endodontics, Dental School of Presidente Prudente, UNOESTE, São Paulo, Brazil. Electronic address: grazielagm@hotmail.com.
authors.forename: Graziela Garrido
authors.initials: GG
authors.lastname: Mori
authors.affiliation: Discipline of Surgery, Dental School of Presidente Prudente, UNOESTE, São Paulo, Brazil.
authors.forename: Ligia Moraes
authors.initials: LM
authors.lastname: Teixeira
authors.affiliation: Dental School of Presidente Prudente, UNOESTE, São Paulo, Brazil.
authors.forename: Danilo Louzada
authors.initials: DL
authors.lastname: de Oliveira
authors.affiliation: Dental School of Presidente Prudente, UNOESTE, São Paulo, Brazil.
authors.forename: Larissa Menegucci
authors.initials: LM
authors.lastname: Jacomini
authors.affiliation: Dental School of Presidente Prudente, UNOESTE, São Paulo, Brazil.
authors.forename: Sindinéia Rodrigues
authors.initials: SR
authors.lastname: da Silva
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D017610:Calcium Compounds / Q000494:pharmacology*; D002481:Cellulitis / Q000473:pathology; D003094:Collagen / Q000032:analysis; D003238:Connective Tissue / Q000187:drug effects / Q000473:pathology; D004338:Drug Combinations; D005347:Fibroblasts / Q000473:pathology; D007509:Irritants / Q000494:pharmacology; D008297:Male; D008422:Materials Testing; D018919:Neovascularization, Physiologic / Q000502:physiology; D009504:Neutrophils / Q000473:pathology; D010087:Oxides / Q000494:pharmacology; D057872:Pulp Capping and Pulpectomy Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*; D040521:Subcutaneous Tissue / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology
chemical_list: D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004338:Drug Combinations; D007509:Irritants; D010087:Oxides; D057872:Pulp Capping and Pulpectomy Agents; D012387:Root Canal Filling Materials; D017640:Silicates; D015035:Zinc Oxide-Eugenol Cement; C086631:mineral trioxide aggregate; C506393:tricalcium silicate; D003094:Collagen
keywords: Biocompatibility; Biodentine; MTA