title: Functional characterization of the small regulatory subunit PetP from the cytochrome b6f complex in Thermosynechococcus elongatus.
abstract: The cyanobacterial cytochrome b(6)f complex is central for the coordination of photosynthetic and respiratory electron transport and also for the balance between linear and cyclic electron transport. The development of a purification strategy for a highly active dimeric b(6)f complex from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 enabled characterization of the structural and functional role of the small subunit PetP in this complex. Moreover, the efficient transformability of this strain allowed the generation of a ΔpetP mutant. Analysis on the whole-cell level by growth curves, photosystem II light saturation curves, and P700(+) reduction kinetics indicate a strong decrease in the linear electron transport in the mutant strain versus the wild type, while the cyclic electron transport via photosystem I and cytochrome b(6)f is largely unaffected. This reduction in linear electron transport is accompanied by a strongly decreased stability and activity of the isolated ΔpetP complex in comparison with the dimeric wild-type complex, which binds two PetP subunits. The distinct behavior of linear and cyclic electron transport may suggest the presence of two distinguishable pools of cytochrome b(6)f complexes with different functions that might be correlated with supercomplex formation.
authors.affiliation: Plant Biochemistry, Faculty of Biology and Biotechnology, Ruhr University Bochum, 44780 Bochum, Germany.
authors.forename: Sascha
authors.identifier: http://orcid.org/0000-0003-3819-2127
authors.initials: S
authors.lastname: Rexroth
authors.affiliation: Plant Biochemistry, Faculty of Biology and Biotechnology, Ruhr University Bochum, 44780 Bochum, Germany.
authors.forename: Dorothea
authors.initials: D
authors.lastname: Rexroth
authors.affiliation: Plant Biochemistry, Faculty of Biology and Biotechnology, Ruhr University Bochum, 44780 Bochum, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Veit
authors.affiliation: Plant Biochemistry, Faculty of Biology and Biotechnology, Ruhr University Bochum, 44780 Bochum, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Plohnke
authors.affiliation: Plant Biochemistry, Faculty of Biology and Biotechnology, Ruhr University Bochum, 44780 Bochum, Germany.
authors.forename: Kai U
authors.initials: KU
authors.lastname: Cormann
authors.affiliation: Plant Biochemistry, Faculty of Biology and Biotechnology, Ruhr University Bochum, 44780 Bochum, Germany.
authors.forename: Marc M
authors.initials: MM
authors.lastname: Nowaczyk
authors.affiliation: Plant Biochemistry, Faculty of Biology and Biotechnology, Ruhr University Bochum, 44780 Bochum, Germany matthias.roegner@rub.de.
authors.forename: Matthias
authors.initials: M
authors.lastname: Rögner
mesh_terms: D000595:Amino Acid Sequence; D049109:Cell Proliferation / Q000235:genetics; D000458:Cyanobacteria / Q000166:cytology / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D045346:Cytochrome b6f Complex / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D019281:Dimerization; D004579:Electron Transport / Q000235:genetics; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010788:Photosynthesis; D045331:Photosystem I Protein Complex / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000378:metabolism; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016415:Sequence Alignment
chemical_list: D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D021122:Protein Subunits; D045346:Cytochrome b6f Complex