title: Localization of amyloid beta (Aβ1-42) protofibrils in membrane lateral compartments: effect of cholesterol and 7-Ketocholesterol.
abstract: Cholesterol plays an important role in the interaction of Alzheimer's amyloid beta (Aβ) with cell membranes, an important event in Aβ-induced cytotoxicity. However, it is not fully understood how cholesterol influences the association of Aβ with membrane lateral compartments. We have shown that by modulating membrane fluidity, cholesterol decreased peptide localization in solid-ordered domains and increased that in liquid-ordered domains. It changed the amount of Aβ associating with liquid-disordered (Ld) phase with different tendencies depending on the composition of heterogeneous membrane systems. 7-Ketocholesterol, an oxidized derivative of cholesterol, majorly enhanced the fluidity of and Aβ interaction with Ld phase. These findings are useful for clarifying the impact of cholesterol and its oxidation in Aβ-induced toxicity.
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan; Hanoi National University of Education, 136 Xuanthuy, Caugiay, Hanoi, Viet Nam.
authors.forename: Huong T T
authors.initials: HT
authors.lastname: Phan
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.
authors.forename: Mun'delanji C
authors.initials: MC
authors.lastname: Vestergaard
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.
authors.forename: KeangOk
authors.initials: K
authors.lastname: Baek
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.
authors.forename: Naofumi
authors.initials: N
authors.lastname: Shimokawa
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan. Electronic address: takagi@jaist.ac.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Takagi
mesh_terms: D000682:Amyloid / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D002784:Cholesterol / Q000502:physiology; D006801:Humans; D019169:Jurkat Cells; D007653:Ketocholesterols / Q000502:physiology*; D008081:Liposomes / Q000378:metabolism; D008560:Membrane Fluidity; D021962:Membrane Microdomains / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism*; D021381:Protein Transport
chemical_list: D000682:Amyloid; D016229:Amyloid beta-Peptides; D007653:Ketocholesterols; D008081:Liposomes; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D002784:Cholesterol; C003001:7-ketocholesterol
keywords: 7-Ketocholesterol; Amyloid beta localization; Cholesterol; Membrane fluidity; Membrane lateral compartments