title: Epidemiological and evolutionary characteristics of the PRRSV in Southern China from 2010 to 2013.
abstract: In 2006, a highly pathogenic strain of porcine reproductive and respiratory syndrome virus (HP-PRRSV) emerged in China and caused lasting damage to the swine industry. To analyze the genetic variation of PRRSV in Southern China, 126 tissue samples were collected; 41 ORF5 and partial Nsp2 genes were sequenced and analyzed. The results showed that the PRRSV positive rate was 32.54% over the last four years, that there are two main subgenotypes in Southern China, and that the dominant strain is HP-PRRSV. An amino acid analysis of Nsp2 showed that 40 strains contained a 30-amino acid deletion in the hypervariable region. However, the 13YJ6-8 mutant exhibited a unique amino acid deletion at positions 508-514 of Nsp2. A phylogenetic analysis of ORF5 revealed that this mutant and five other strains, belong to an intermediate subgenotype (inter-subgenotype), which is characterized by extensive mutations, especially in the signal peptide and N-glycosylation sites. The results of this study demonstrate the genetic diversity of PRRSV in Southern China and provide basic knowledge of the PRRSV epidemic in this region.
authors.affiliation: MOA Key Laboratory of Animal Vaccine Development, Ministry of China, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Jiexiong
authors.initials: J
authors.lastname: Xie
authors.affiliation: MOA Key Laboratory of Animal Vaccine Development, Ministry of China, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Cui
authors.affiliation: MOA Key Laboratory of Animal Vaccine Development, Ministry of China, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Cui
authors.affiliation: MOA Key Laboratory of Animal Vaccine Development, Ministry of China, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: MOA Key Laboratory of Animal Vaccine Development, Ministry of China, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Minze
authors.initials: M
authors.lastname: Zhang
authors.affiliation: MOA Key Laboratory of Animal Vaccine Development, Ministry of China, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Zhou
authors.affiliation: MOA Key Laboratory of Animal Vaccine Development, Ministry of China, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Shengchao
authors.initials: S
authors.lastname: Deng
authors.affiliation: MOA Key Laboratory of Animal Vaccine Development, Ministry of China, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. Electronic address: ssh5658485@163.com.
authors.forename: Shuo
authors.initials: S
authors.lastname: Su
authors.affiliation: MOA Key Laboratory of Animal Vaccine Development, Ministry of China, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. Electronic address: guihongzh@scau.edu.cn.
authors.forename: Guihong
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002681:China; D016000:Cluster Analysis; D019143:Evolution, Molecular; D014644:Genetic Variation; D005838:Genotype; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D019318:Porcine Reproductive and Respiratory Syndrome / Q000453:epidemiology* / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D017385:Sequence Homology; D013552:Swine; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins
keywords: GP5; Molecular epidemiology; Nsp2; Porcine reproductive and respiratory syndrome virus (PRRSV); South China