title: Evaluation of the nanosphere Verigene BC-GN assay for direct identification of gram-negative bacilli and antibiotic resistance markers from positive blood cultures and potential impact for more-rapid antibiotic interventions.
abstract: The Verigene BC-GN assay correctly identified all 51 Gram-negative bacilli (GNB) from positive blood cultures and all 14 carbapenemase enzymes tested. The assay gave organism identification (ID) results an average of 24 h faster compared to conventional identifications. Medical management could have been modified for 31.8% of patients an average 33 h sooner. In conclusion, the BC-GN assay is a very accurate, rapid assay which would allow for more-immediate medical management decisions in patients with bacteremia from GNB.
authors.affiliation: Microbiology/Molecular Department, Kaiser Permanente, Sunnyside Medical Center, Portland, Oregon, USA.
authors.forename: Joseph T
authors.initials: JT
authors.lastname: Hill
authors.affiliation: Microbiology/Molecular Department, Kaiser Permanente, Sunnyside Medical Center, Portland, Oregon, USA.
authors.forename: Kim-Dung T
authors.initials: KD
authors.lastname: Tran
authors.affiliation: Regional Antimicrobial Stewardship Program, Kaiser Permanente, Sunnyside Medical Center, Portland, Oregon, USA.
authors.forename: Karen L
authors.initials: KL
authors.lastname: Barton
authors.affiliation: Regional Antimicrobial Stewardship Program, Kaiser Permanente, Sunnyside Medical Center, Portland, Oregon, USA.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Labreche
authors.affiliation: Microbiology/Molecular Department, Kaiser Permanente, Sunnyside Medical Center, Portland, Oregon, USA susan.e.sharp@kp.org.
authors.forename: Susan E
authors.initials: SE
authors.lastname: Sharp
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use; D016470:Bacteremia / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D001769:Blood / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D005819:Genetic Markers; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D054118:Nanospheres; D012189:Retrospective Studies; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D005819:Genetic Markers