title: Novel methodologies in analysis of small molecule biomarkers and living cells.
abstract: Enzyme-linked immuno-sorbent assay (ELISA) is widely used for biomarker detection. A good biomarker can distinguish patients from healthy or benign diseases. However, the ELISA method is not suitable for small molecule or trace substance detection. Along with the development of new technologies, an increasing level of biomaterials, especially small molecules, will be identified as novel biomarkers. Quantitative immuno-PCR, chromatography-mass spectrometry, and nucleic acid aptamer are emerging methodologies for detection of small molecule biomarkers, even in living cells. In this review, we focus on these novel technologies and their potential for small molecule biomarkers and living cell analysis.
authors.affiliation: Department of Surgery, Shanghai Institute of Digestive Surgery, Shanghai Key Laboratory for Gastric Neoplasms, Shanghai Ruijin Hospital, Shanghai Jiao Tong University, School of Medicine, Ruijin er Road, No. 197, 200025, Shanghai, China.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhenggang
authors.initials: Z
authors.lastname: Zhu
authors.forename: Yingyan
authors.initials: Y
authors.lastname: Yu
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000032:analysis; D015415:Biomarkers / Q000032:analysis*; D002470:Cell Survival; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D052156:SELEX Aptamer Technique
chemical_list: D052157:Aptamers, Nucleotide; D015415:Biomarkers