title: The global regulator FfSge1 is required for expression of secondary metabolite gene clusters but not for pathogenicity in Fusarium fujikuroi.
abstract: The plant pathogenic fungus Fusarium fujikuroi is the causal agent of bakanae disease on rice due to its ability to produce gibberellins. Besides these phytohormones, F. fujikuroi is able to produce several other secondary metabolites (SMs). Although much progress has been made in the field of secondary metabolism, the transcriptional regulation of SM biosynthesis is complex and still incompletely understood. Environmental conditions, global as well as pathway-specific regulators and chromatin remodelling have been shown to play major roles. Here, the role of FfSge1, a homologue of the morphological switch regulators Wor1 and Ryp1 in Candida albicans and Histoplasma capsulatum, respectively, is explored with emphasis on secondary metabolism. FfSge1 is not required for formation of conidia and pathogenicity but is involved in vegetative growth. Transcriptome analysis of the mutant Δffsge1 compared with the wild type, as well as comparative chemical analysis between the wild type, Δffsge1 and OE:FfSGE1, revealed that FfSge1 functions as a global activator of secondary metabolism in F. fujikuroi. Double mutants of FfSGE1 and other SM regulatory genes brought insights into the hierarchical regulation of secondary metabolism. In addition, FfSge1 is also required for expression of a yet uncharacterized SM gene cluster containing a non-canonical non-ribosomal peptide synthetase.
authors.affiliation: Institute of Biology and Biotechnology of Plants, Westfälische Wilhelms-University, Schlossplatz 8, Münster, 48143, Germany.
authors.forename: Caroline B
authors.initials: CB
authors.lastname: Michielse
authors.affiliation: Institute of Biology and Biotechnology of Plants, Westfälische Wilhelms-University, Schlossplatz 8, Münster, 48143, Germany.
authors.forename: Lena
authors.initials: L
authors.lastname: Studt
authors.affiliation: Institute of Biology and Biotechnology of Plants, Westfälische Wilhelms-University, Schlossplatz 8, Münster, 48143, Germany.
authors.forename: Slavica
authors.initials: S
authors.lastname: Janevska
authors.affiliation: Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, Germany Research Center for Environmental Health (GmbH), Ingolstädter Landstr. 1, Neuherberg, 85764, Germany.
authors.forename: Christian M K
authors.initials: CM
authors.lastname: Sieber
authors.affiliation: NRW Graduate School of Chemistry, Westfälische Wilhelms-University, Wilhelm-Klemm-Strasse 10, Münster, 48149, Germany.|Institute of Food Chemistry, Westfälische Wilhelms-University, Corrensstr. 45, Münster, 48149, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Arndt
authors.affiliation: Institute of Biology and Biotechnology of Plants, Westfälische Wilhelms-University, Schlossplatz 8, Münster, 48143, Germany.
authors.forename: Jose Juan
authors.initials: JJ
authors.lastname: Espino
authors.affiliation: NRW Graduate School of Chemistry, Westfälische Wilhelms-University, Wilhelm-Klemm-Strasse 10, Münster, 48149, Germany.|Institute of Food Chemistry, Westfälische Wilhelms-University, Corrensstr. 45, Münster, 48149, Germany.
authors.forename: Hans-Ulrich
authors.initials: HU
authors.lastname: Humpf
authors.affiliation: Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, Germany Research Center for Environmental Health (GmbH), Ingolstädter Landstr. 1, Neuherberg, 85764, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Güldener
authors.affiliation: Institute of Biology and Biotechnology of Plants, Westfälische Wilhelms-University, Schlossplatz 8, Münster, 48143, Germany.
authors.forename: Bettina
authors.initials: B
authors.lastname: Tudzynski
mesh_terms: D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005670:Fusarium / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D012275:Oryza / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology; D064210:Secondary Metabolism / Q000235:genetics*; D014774:Virulence / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D026901:Membrane Transport Proteins