title: Prevalence and molecular epidemiology of methicillin-resistant Staphylococcus aureus in a tertiary Greek hospital.
abstract: OBJECTIVE
  Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of health- and community-associated infections; its prevalence in Greece is among the highest in Europe. We investigated the prevalence and molecular epidemiology of MRSA in a tertiary Greek hospital.
  
  
  METHODS
  Spa typing and random polymorphic DNA analysis were used to investigate the molecular epidemiology of 28 MRSA isolates during May 2010 to May 2011 in a tertiary hospital in Northern Greece.
  
  
  RESULTS
  Nine spa types were detected; t003 was the predominant (32.1%) one, detected in various wards and throughout the study period, while t037 was recovered only from intensive care unit patients, and only in April 2011, suggestive of an epidemic. Additional rare types were detected for the first time in Greece.
  
  
  CONCLUSIONS
  Spa typing and random polymorphic DNA analysis gave an insight into the epidemiology of MRSA in a Northern Greece hospital. Concerning the distribution in the hospital, the predominant spa type t003 was present in various wards, and was constantly detected throughout the study period, very suggestive of an epidemic, while other types were detected only in specific wards. Our data underline the need for surveillance, typing and constant reassessment of existing strategies to control MRSA.
authors.affiliation: 1 Department of Microbiology, Medical School, Aristotle University of Thessaloniki, Greece ; Department of Microbiology, of George Papanikolaou Hospital, Thessaloniki, Greece.
authors.forename: M
authors.initials: M
authors.lastname: Kachrimanidou
authors.affiliation: Department of Microbiology, of George Papanikolaou Hospital, Thessaloniki, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Tsorlini
authors.affiliation: Department of Microbiology, of George Papanikolaou Hospital, Thessaloniki, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Katsifa
authors.affiliation: Department of Microbiology, of George Papanikolaou Hospital, Thessaloniki, Greece.
authors.forename: S
authors.initials: S
authors.lastname: Vlachou
authors.affiliation: Department of Microbiology, of George Papanikolaou Hospital, Thessaloniki, Greece.
authors.forename: S
authors.initials: S
authors.lastname: Kyriakidou
authors.affiliation: 1 Department of Microbiology, Medical School, Aristotle University of Thessaloniki, Greece.
authors.forename: K
authors.initials: K
authors.lastname: Xanthopoulou
authors.affiliation: 1 Department of Microbiology, Medical School, Aristotle University of Thessaloniki, Greece.
authors.forename: K
authors.initials: K
authors.lastname: Tsergouli
authors.affiliation: Department of Microbiology, of George Papanikolaou Hospital, Thessaloniki, Greece.
authors.forename: T
authors.initials: T
authors.lastname: Samourli
authors.affiliation: 1 Department of Microbiology, Medical School, Aristotle University of Thessaloniki, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Papa
keywords: Greece; MRSA; Staphylococcus aureus; spatyping