title: Synthesis, characterization, and antimicrobial activity of an ampicillin-conjugated magnetic nanoantibiotic for medical applications.
abstract: Because of their magnetic properties, magnetic nanoparticles (MNPs) have numerous diverse biomedical applications. In addition, because of their ability to penetrate bacteria and biofilms, nanoantimicrobial agents have become increasingly popular for the control of infectious diseases. Here, MNPs were prepared through an iron salt coprecipitation method in an alkaline medium, followed by a chitosan coating step (CS-coated MNPs); finally, the MNPs were loaded with ampicillin (amp) to form an amp-CS-MNP nanocomposite. Both the MNPs and amp-CS-MNPs were subsequently characterized and evaluated for their antibacterial activity. X-ray diffraction results showed that the MNPs and nanocomposites were composed of pure magnetite. Fourier transform infrared spectra and thermogravimetric data for the MNPs, CS-coated MNPs, and amp-CS-MNP nanocomposite were compared, which confirmed the CS coating on the MNPs and the amp-loaded nanocomposite. Magnetization curves showed that both the MNPs and the amp-CS-MNP nanocomposites were superparamagnetic, with saturation magnetizations at 80.1 and 26.6 emu g(-1), respectively. Amp was loaded at 8.3%. Drug release was also studied, and the total release equilibrium for amp from the amp-CS-MNPs was 100% over 400 minutes. In addition, the antimicrobial activity of the amp-CS-MNP nanocomposite was determined using agar diffusion and growth inhibition assays against Gram-positive bacteria and Gram-negative bacteria, as well as Candida albicans. The minimum inhibitory concentration of the amp-CS-MNP nanocomposite was determined against bacteria including Mycobacterium tuberculosis. The synthesized nanocomposites exhibited antibacterial and antifungal properties, as well as antimycobacterial effects. Thus, this study introduces a novel β-lactam antibacterial-based nanocomposite that can decrease fungus activity on demand for numerous medical applications.
authors.affiliation: Laboratory of Molecular Biomedicine, Isra University, Amman, Kingdom of Jordan, Jordan ; Faculty of Pharmacy, Isra University, Amman, Kingdom of Jordan, Jordan.
authors.forename: Samer Hasan
authors.initials: SH
authors.lastname: Hussein-Al-Ali
authors.affiliation: Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia ; Faculty of Public Health and Tropical Medicine, Jazan University, Jazan, Kingdom of Saudi Arabia.
authors.forename: Mohamed Ezzat
authors.initials: ME
authors.lastname: El Zowalaty
authors.affiliation: Materials Synthesis and Characterization Laboratory Institute of Advanced Technology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Mohd Zobir
authors.initials: MZ
authors.lastname: Hussein
authors.affiliation: Department of Chemical Engineering and Program in Bioengineering, Northeastern University, Boston, MA, USA.
authors.forename: Benjamin M
authors.initials: BM
authors.lastname: Geilich
authors.affiliation: Department of Chemical Engineering and Program in Bioengineering, Northeastern University, Boston, MA, USA ; Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Webster
mesh_terms: D000667:Ampicillin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D053761:Nanocomposites / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D058185:Magnetite Nanoparticles; D000667:Ampicillin; D048271:Chitosan
keywords: and nanoantibiotics; antibacterial activity; chitosan; coating material; iron oxide nanoparticles; β-lactam