title: A novel modified electrode as GC/PPy-AuNPs-rGO/L-Cys/Ag@MUA nanostructure configuration for determination of CCP and CRP antibodies in human blood serum samples.
abstract: In this work, silver nanoparticles were synthesized and stabilized with 11-mercaptoundecanoateanions to produce a new Ag@MUA core shell structure, and its utilizing for fabrication of a new sensing film. Gold nanoparticles (AuNPs) were electrochemically produced and simultaneously immobilized into the electropolymerized polypyrrole (PPy) film with the reduced graphene oxide (rGO). The Ag@MUA was then grafted to the surface of GC/PPy-AuNPs-rGO film using L-cysteine (L-Cys) linker agent and trifluoromethanesulfonic anhydride (TF2O), at ambient temperature and under the electrode stirring. The characterization of the sensor was studied by scanning electron microscopy, electrochemical impedance spectroscopy, cyclic and square wave voltammetry techniques. The utility of the modified electrode for clinical diagnosis has been successfully demonstrated by the analysis of human blood serums with a certified CRP and CCP content. Thus, the proposed sensor shows simple preparation, accuracy and precision in the analysis of cytochrome c protein (CCP) and C-reactive protein (CRP (with less side interferences.
authors.affiliation: Department of Chemistry, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran; Department of Food Chemical Engineering, Kermanshah Science and Research Branch, Islamic Azad University, Kermanshah, Iran. Electronic address: arash.babakhanian@gmail.com.
authors.forename: Arash
authors.initials: A
authors.lastname: Babakhanian
authors.affiliation: Department of Chemistry, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran.
authors.forename: Hosna
authors.initials: H
authors.lastname: Ehzari
authors.affiliation: Department of Food Chemical Engineering, Kermanshah Science and Research Branch, Islamic Azad University, Kermanshah, Iran.
authors.forename: Samineh
authors.initials: S
authors.lastname: Kaki
authors.affiliation: Department of Chemistry, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Hamidi
keywords: C-reactive protein (CRP); Cyclic voltametry (CV); Cytochrome c protein (CCP); Electrochemical impedance spectroscopy (EIS); Glassy carbon (GC) electrode; Modified electrode