title: Development of phage immuno-loop-mediated isothermal amplification assays for organophosphorus pesticides in agro-products.
abstract: Two immuno-loop-mediated isothermal amplification assays (iLAMP) were developed by using a phage-borne peptide that was isolated from a cyclic eight-peptide phage library. One assay was used to screen eight organophosphorus (OP) pesticides with limits of detection (LOD) between 2 and 128 ng mL(-1). The iLAMP consisted of the competitive immuno-reaction coupled to the LAMP reaction for detection. This method provides positive results in the visual color of violet, while a negative response results in a sky blue color; therefore, the iLAMP allows one to rapidly detect analytes in yes or no fashion. We validated the iLAMP by detecting parathion-methyl, parathion, and fenitrothion in Chinese cabbage, apple, and greengrocery, and the detection results were consistent with the enzyme-linked immunosorbent assay (ELISA). In conclusion, the iLAMP is a simple, rapid, sensitive, and economical method for detecting OP pesticide residues in agro-products with no instrumental requirement.
authors.affiliation: College of Plant Protection (State & Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University , Nanjing 210095, P.R. China.
authors.forename: Xiude
authors.initials: X
authors.lastname: Hua
authors.forename: Wei
authors.initials: W
authors.lastname: Yin
authors.forename: Haiyan
authors.initials: H
authors.lastname: Shi
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Yanru
authors.initials: Y
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Yonghao
authors.initials: Y
authors.lastname: Ye
authors.forename: Hee Joo
authors.initials: HJ
authors.lastname: Kim
authors.forename: Shirley J
authors.initials: SJ
authors.lastname: Gee
authors.forename: Minghua
authors.initials: M
authors.lastname: Wang
authors.forename: Fengquan
authors.initials: F
authors.lastname: Liu
authors.forename: Bruce D
authors.initials: BD
authors.lastname: Hammock
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001435:Bacteriophages / Q000737:chemistry*; D001483:Base Sequence; D001937:Brassica / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D005504:Food Analysis / Q000191:economics / Q000379:methods*; D007118:Immunoassay / Q000191:economics / Q000379:methods*; D057230:Limit of Detection; D027845:Malus / Q000737:chemistry; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000191:economics / Q000379:methods; D009943:Organophosphorus Compounds / Q000032:analysis*; D010455:Peptides / Q000737:chemistry*; D010575:Pesticides / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D009943:Organophosphorus Compounds; D010455:Peptides; D010575:Pesticides; D004247:DNA