title: A novel zinc-finger HIT protein with an additional PAPA-1-like region from Suaeda liaotungensis K. enhanced transgenic Arabidopsis drought and salt stresses tolerance.
abstract: Zinc-finger HIT belongs to the cross-brace zinc finger protein family and is involved in the regulation of plant defense and stress responses. In this study, we cloned a full-length zinc-finger HIT gene (1,377 bp) named SlPAPA1 using polymerase chain reaction from Suaeda liaotungensis K. and investigated its function by overexpression in transgenic Arabidopsis. SlPAPA1 contains a zinc-finger HIT domain and a Pim-1-associated protein-1 (PAP-1)-associated protein-1-like (PAPA-1-like) conserved region. Its expression in S. liaotungensis was induced by drought, high-salt, and cold (4 °C) stresses and by abscisic acid (ABA). Subcellular localization experiments in onion epidermal cells indicated that SlPAPA1 is localized in the nucleus. Yeast-one hybrid assays showed that SlPAPA1 functions as a transcriptional activator. SlPAPA1 transgenic Arabidopsis displayed a higher survival ratio and lower rate of water loss under drought stress; a higher germination ratio, higher survival ratio, and lower root inhibition rate under salt stress; and a lower germination ratio and root inhibition rate under ABA treatment, compared with wild-type Arabidopsis. These results suggested that SlPAPA1 functions as a stress-responsive zinc-finger HIT protein involved in the ABA-dependent signaling pathway and may have potential applications in transgenic breeding to enhance crops abiotic stress tolerances.
authors.affiliation: College of Life Sciences, Liaoning Normal University and Key Laboratory of Plant Biotechnology of Liaoning Province, 1 South Liushu Street, Ganjingzi District, Dalian, 116081, Liaoning, China, lixiaolanl@163.com.
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Li
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Hu
authors.forename: Xing
authors.initials: X
authors.lastname: Yang
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Yu
authors.forename: Qiu-Li
authors.initials: QL
authors.lastname: Li
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D019663:Chenopodiaceae / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D003080:Cold Temperature; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D000073718:Pancreatitis-Associated Proteins; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D012492:Salts; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D000073718:Pancreatitis-Associated Proteins; D010940:Plant Proteins; C000612141:REG3A protein, human; D012492:Salts; D014157:Transcription Factors