title: Tryptophan hydroxylase 2 gene polymorphism in anxiety and depressive disorder in kashmiri population.
abstract: BACKGROUND
  The gene of tryptophan hydroxylase is widely recognized as a major candidate gene in many psychiatric disorders. However, no study has been done which investigates tryptophan hydroxylase 2 gene polymorphism in anxiety and depressive disorders in Kashmiri population (India).
  
  
  OBJECTIVE
  To study tryptophan hydroxylase 2 (TPH2) C 11993 A gene polymorphism in anxiety and depressive disorders.
  
  
  METHODS
  Sixty patients of depression disorder, 60 patients of anxiety disorder and 40 unrelated healthy volunteers (control) were studied in a case control design. Polymorphism was determined using polymerase chain reaction (PCR) and agarose gel electrophoresis after digestion with HAP II enzyme. Genotypes and allele frequencies were compared using Chi-square tests, Fischer's exact test, odds ratio, 95% confidence interval (C.I) and p-value of <0.05 was considered to be statistical significant.
  
  
  RESULTS
  The mean age ± SD of anxiety, depression and control group was 32.73±10.99, 32.20±10 and 29.75±10.12 respectively and the difference was found to be statistically non significant (p=0.349).The mean HAM-A (Hamilton rating scale for anxiety) score and HAM-D (Hamilton rating scale for depression) score was high in both groups (anxiety and depression) and found to be statistically significant (p=0.001).Depression group had AA genotype (55.2%) than control (37.5%) and was found to be statistically non significant (p=0.890).Comparison of allelic frequency revealed no association of A allele in anxiety group (76.67%) compared with control (75.5%) and was found to be statistically non significant (p= 0.866), OR 1.09 (0.56-2.11).
  
  
  CONCLUSIONS
  TPH2C 11993 A gene was not found to be associated with major depressive disorder (MDD) and anxiety disorder in Kashmiri population.
authors.affiliation: Registrar, Department of Psychiatry, Government Medical College , Srinagar, Kashmir, India .
authors.forename: Raheel
authors.initials: R
authors.lastname: Mushtaq
authors.affiliation: Registrar, Department of Psychiatry, Government Medical College , Srinagar, Kashmir, India .
authors.forename: Sheikh
authors.initials: S
authors.lastname: Shoib
authors.affiliation: Intern, Government Medical College , Srinagar, Kashmir, India .
authors.forename: Tabindah
authors.initials: T
authors.lastname: Shah
authors.affiliation: Student, Acharya Shri Chander College of Medical Sciences , Sidhra, Jammu, Kashmir, India .
authors.forename: Sahil
authors.initials: S
authors.lastname: Mushtaq
keywords: HAM-A; HAM-D; Psychiatric disorders