title: Large-scale identification of gibberellin-related transcription factors defines group VII ETHYLENE RESPONSE FACTORS as functional DELLA partners.
abstract: DELLA proteins are the master negative regulators in gibberellin (GA) signaling acting in the nucleus as transcriptional regulators. The current view of DELLA action indicates that their activity relies on the physical interaction with transcription factors (TFs). Therefore, the identification of TFs through which DELLAs regulate GA responses is key to understanding these responses from a mechanistic point of view. Here, we have determined the TF interactome of the Arabidopsis (Arabidopsis thaliana) DELLA protein GIBBERELLIN INSENSITIVE and screened a collection of conditional TF overexpressors in search of those that alter GA sensitivity. As a result, we have found RELATED TO APETALA2.3, an ethylene-induced TF belonging to the group VII ETHYLENE RESPONSE FACTOR of the APETALA2/ethylene responsive element binding protein superfamily, as a DELLA interactor with physiological relevance in the context of apical hook development. The combination of transactivation assays and chromatin immunoprecipitation indicates that the interaction with GIBBERELLIN INSENSITIVE impairs the activity of RELATED TO APETALA2.3 on the target promoters. This mechanism represents a unique node in the cross regulation between the GA and ethylene signaling pathways controlling differential growth during apical hook development.
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, 46022 Valencia, Spain (N.M.-d.l.R., B.S., D.A., M.A.B.);Department of Forest Genetics and Plant Physiology, Umeå Plant Science Center, Swedish University of Agricultural Sciences, 90187 Umea, Sweden (P.M., R.B.);Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom (D.J.G., J.V., M.J.H.);Centro de Biotecnología y Genómica de Plantas, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Campus de Montegancedo, 28223 Pozuelo de Alarcón, Spain (P.C., L.O.-S.); andCollege of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia (R.B.).
authors.forename: Nora
authors.initials: N
authors.lastname: Marín-de la Rosa
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, 46022 Valencia, Spain (N.M.-d.l.R., B.S., D.A., M.A.B.);Department of Forest Genetics and Plant Physiology, Umeå Plant Science Center, Swedish University of Agricultural Sciences, 90187 Umea, Sweden (P.M., R.B.);Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom (D.J.G., J.V., M.J.H.);Centro de Biotecnología y Genómica de Plantas, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Campus de Montegancedo, 28223 Pozuelo de Alarcón, Spain (P.C., L.O.-S.); andCollege of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia (R.B.).
authors.forename: Berta
authors.initials: B
authors.lastname: Sotillo
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, 46022 Valencia, Spain (N.M.-d.l.R., B.S., D.A., M.A.B.);Department of Forest Genetics and Plant Physiology, Umeå Plant Science Center, Swedish University of Agricultural Sciences, 90187 Umea, Sweden (P.M., R.B.);Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom (D.J.G., J.V., M.J.H.);Centro de Biotecnología y Genómica de Plantas, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Campus de Montegancedo, 28223 Pozuelo de Alarcón, Spain (P.C., L.O.-S.); andCollege of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia (R.B.).
authors.forename: Pal
authors.identifier: http://orcid.org/0000-0002-1036-5831
authors.initials: P
authors.lastname: Miskolczi
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, 46022 Valencia, Spain (N.M.-d.l.R., B.S., D.A., M.A.B.);Department of Forest Genetics and Plant Physiology, Umeå Plant Science Center, Swedish University of Agricultural Sciences, 90187 Umea, Sweden (P.M., R.B.);Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom (D.J.G., J.V., M.J.H.);Centro de Biotecnología y Genómica de Plantas, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Campus de Montegancedo, 28223 Pozuelo de Alarcón, Spain (P.C., L.O.-S.); andCollege of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia (R.B.).
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Gibbs
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, 46022 Valencia, Spain (N.M.-d.l.R., B.S., D.A., M.A.B.);Department of Forest Genetics and Plant Physiology, Umeå Plant Science Center, Swedish University of Agricultural Sciences, 90187 Umea, Sweden (P.M., R.B.);Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom (D.J.G., J.V., M.J.H.);Centro de Biotecnología y Genómica de Plantas, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Campus de Montegancedo, 28223 Pozuelo de Alarcón, Spain (P.C., L.O.-S.); andCollege of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia (R.B.).
authors.forename: Jorge
authors.initials: J
authors.lastname: Vicente
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, 46022 Valencia, Spain (N.M.-d.l.R., B.S., D.A., M.A.B.);Department of Forest Genetics and Plant Physiology, Umeå Plant Science Center, Swedish University of Agricultural Sciences, 90187 Umea, Sweden (P.M., R.B.);Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom (D.J.G., J.V., M.J.H.);Centro de Biotecnología y Genómica de Plantas, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Campus de Montegancedo, 28223 Pozuelo de Alarcón, Spain (P.C., L.O.-S.); andCollege of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia (R.B.).
authors.forename: Pilar
authors.initials: P
authors.lastname: Carbonero
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, 46022 Valencia, Spain (N.M.-d.l.R., B.S., D.A., M.A.B.);Department of Forest Genetics and Plant Physiology, Umeå Plant Science Center, Swedish University of Agricultural Sciences, 90187 Umea, Sweden (P.M., R.B.);Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom (D.J.G., J.V., M.J.H.);Centro de Biotecnología y Genómica de Plantas, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Campus de Montegancedo, 28223 Pozuelo de Alarcón, Spain (P.C., L.O.-S.); andCollege of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia (R.B.).
authors.forename: Luis
authors.initials: L
authors.lastname: Oñate-Sánchez
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, 46022 Valencia, Spain (N.M.-d.l.R., B.S., D.A., M.A.B.);Department of Forest Genetics and Plant Physiology, Umeå Plant Science Center, Swedish University of Agricultural Sciences, 90187 Umea, Sweden (P.M., R.B.);Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom (D.J.G., J.V., M.J.H.);Centro de Biotecnología y Genómica de Plantas, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Campus de Montegancedo, 28223 Pozuelo de Alarcón, Spain (P.C., L.O.-S.); andCollege of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia (R.B.).
authors.forename: Michael J
authors.identifier: http://orcid.org/0000-0002-3954-9215
authors.initials: MJ
authors.lastname: Holdsworth
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, 46022 Valencia, Spain (N.M.-d.l.R., B.S., D.A., M.A.B.);Department of Forest Genetics and Plant Physiology, Umeå Plant Science Center, Swedish University of Agricultural Sciences, 90187 Umea, Sweden (P.M., R.B.);Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom (D.J.G., J.V., M.J.H.);Centro de Biotecnología y Genómica de Plantas, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Campus de Montegancedo, 28223 Pozuelo de Alarcón, Spain (P.C., L.O.-S.); andCollege of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia (R.B.).
authors.forename: Rishikesh
authors.initials: R
authors.lastname: Bhalerao
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, 46022 Valencia, Spain (N.M.-d.l.R., B.S., D.A., M.A.B.);Department of Forest Genetics and Plant Physiology, Umeå Plant Science Center, Swedish University of Agricultural Sciences, 90187 Umea, Sweden (P.M., R.B.);Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom (D.J.G., J.V., M.J.H.);Centro de Biotecnología y Genómica de Plantas, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Campus de Montegancedo, 28223 Pozuelo de Alarcón, Spain (P.C., L.O.-S.); andCollege of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia (R.B.) dalabadi@ibmcp.upv.es.
authors.forename: David
authors.identifier: http://orcid.org/0000-0001-8492-6713
authors.initials: D
authors.lastname: Alabadí
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, 46022 Valencia, Spain (N.M.-d.l.R., B.S., D.A., M.A.B.);Department of Forest Genetics and Plant Physiology, Umeå Plant Science Center, Swedish University of Agricultural Sciences, 90187 Umea, Sweden (P.M., R.B.);Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough LE12 5RD, United Kingdom (D.J.G., J.V., M.J.H.);Centro de Biotecnología y Genómica de Plantas, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Campus de Montegancedo, 28223 Pozuelo de Alarcón, Spain (P.C., L.O.-S.); andCollege of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia (R.B.).
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Blázquez
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers; D005875:Gibberellins / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D029681:Arabidopsis Proteins; D017931:DNA Primers; C109555:GAI protein, Arabidopsis; D005875:Gibberellins; D014157:Transcription Factors