title: Treatment of FANCA cells with resveratrol and N-acetylcysteine: a comparative study.
abstract: Fanconi anemia (FA) is a genetic disorder characterised by chromosome instability, cytokine ipersensibility, bone marrow failure and abnormal haematopoiesis associated with acute myelogenous leukemia. Recent reports are contributing to characterize the peculiar FA metabolism. Central to these considerations appears that cells from complementation group A (FANCA) display an altered red-ox metabolism. Consequently the possibility to improve FA phenotypical conditions with antioxidants is considered. We have characterized from the structural and biochemical point of view the response of FANCA lymphocytes to N-acetyl-cysteine (NAC) and resveratrol (RV). Surprisingly both NAC and RV failed to revert all the characteristic of FA phenotype and moreover their effects are not super imposable. Our data suggest that we must be aware of the biological effects coming from antioxidant treatment.
authors.affiliation: SC Laboratory of Musculoskeletal Cell Biology, IOR, Bologna, Italy.
authors.forename: Marta
authors.initials: M
authors.lastname: Columbaro
authors.affiliation: DIFAR-Biochemistry Lab., University of Genova, Genova, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Ravera
authors.affiliation: CNR-National Research Council of Italy, Institute of Molecular Genetics, Unit of Bologna-IOR, Bologna, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Capanni
authors.affiliation: DIFAR-Biochemistry Lab., University of Genova, Genova, Italy.
authors.forename: Isabella
authors.initials: I
authors.lastname: Panfoli
authors.affiliation: S. C. Mutagenesis, IRCCS AOU San Martino - IST (Istituto Nazionale per la Ricerca sul Cancro), Genova, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Cuccarolo
authors.affiliation: Centro di Diagnostica Genetica e Biochimica delle Malattie Metaboliche, Istituto Giannina Gaslini, Genova, Italy.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Stroppiana
authors.affiliation: S. C. Mutagenesis, IRCCS AOU San Martino - IST (Istituto Nazionale per la Ricerca sul Cancro), Genova, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Degan
authors.affiliation: Hematology Unit, Istituto Giannina Gaslini, Genova, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Cappelli
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D005199:Fanconi Anemia / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D008928:Mitochondria / Q000473:pathology; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D013267:Stilbenes; D000077185:Resveratrol; D000111:Acetylcysteine