title: Reassessment of genotype 1 hepatitis C virus subtype misclassification by LiPA 2.0: implications for direct-acting antiviral treatment.
abstract: The accuracy of LiPA 2.0 for hepatitis C virus 1 (HCV-1) subtype classification was analyzed. LiPA 2.0 genotype results from 101 HCV-1-infected patients were compared to genotype findings determined by direct core sequencing. Eleven (11%) samples were misclassified. Given the influence of the HCV-1-subtype in the anti-HCV therapy response, an alternative classification method is warranted.
authors.affiliation: Unidad de Enfermedades Infecciosas y Microbiología, Hospital Universitario de Valme, Seville, Spain Instituto de Biomedicina de Seville (IBIS), Seville, Spain.
authors.forename: Javier R
authors.initials: JR
authors.lastname: Guelfo
authors.affiliation: Unidad de Enfermedades Infecciosas y Microbiología, Hospital Universitario de Valme, Seville, Spain Instituto de Biomedicina de Seville (IBIS), Seville, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Macias
authors.affiliation: Unidad de Enfermedades Infecciosas y Microbiología, Hospital Universitario de Valme, Seville, Spain Instituto de Biomedicina de Seville (IBIS), Seville, Spain.
authors.forename: Karin
authors.initials: K
authors.lastname: Neukam
authors.affiliation: Unidad de Enfermedades Infecciosas y Microbiología, Hospital Universitario de Valme, Seville, Spain Cátedra de Virología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Federico A
authors.initials: FA
authors.lastname: Di Lello
authors.affiliation: Unidad de Enfermedades Infecciosas y Microbiología, Hospital Universitario de Valme, Seville, Spain Instituto de Biomedicina de Seville (IBIS), Seville, Spain.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Mira
authors.affiliation: Unidad de Enfermedades Infecciosas y Microbiología, Hospital Universitario de Valme, Seville, Spain Instituto de Biomedicina de Seville (IBIS), Seville, Spain.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Merchante
authors.affiliation: Unidad de Enfermedades Infecciosas y Microbiología, Hospital Universitario de Valme, Seville, Spain Instituto de Biomedicina de Seville (IBIS), Seville, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Mancebo
authors.affiliation: Unidad de Enfermedades Infecciosas y Microbiología, Hospital Universitario de Valme, Seville, Spain Instituto de Biomedicina de Seville (IBIS), Seville, Spain.
authors.forename: Rocío
authors.initials: R
authors.lastname: Nuñez-Torres
authors.affiliation: Unidad de Enfermedades Infecciosas y Microbiología, Hospital Universitario de Valme, Seville, Spain Instituto de Biomedicina de Seville (IBIS), Seville, Spain.
authors.forename: Juan A
authors.initials: JA
authors.lastname: Pineda
authors.affiliation: Unidad de Enfermedades Infecciosas y Microbiología, Hospital Universitario de Valme, Seville, Spain Instituto de Biomedicina de Seville (IBIS), Seville, Spain lmreal67b@gmail.com.
authors.forename: Luis M
authors.initials: LM
authors.lastname: Real
mesh_terms: D000328:Adult; D000998:Antiviral Agents / Q000627:therapeutic use*; D005260:Female; D005838:Genotype; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D019698:Hepatitis C, Chronic / Q000188:drug therapy / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000998:Antiviral Agents; D012367:RNA, Viral