title: Complement membrane attack complex is related with immune-mediated necrotizing myopathy.
abstract: This study is to investigate the expression of complement membrane attack complex (C5b-9) in the skeletal muscle of patients with necrotizing myopathy (NM), and to investigate the relationship between C5b-9 and NM. Thirteen patients with NM and control patients with polymyositis and muscular dystrophy were enrolled in this study. Examinations including creatine kinase (CK) and L-lactate dehydrogenase (LDH) in the serum, electromyogram and muscle pathological examination were performed. C5b-9 expression in the skeletal muscle was determined by immunohistochemistry and analyzed by Image Plus Pro 6.0. C5b-9 expression was particularly prominent in necrotic muscle fibers, and also positive in blood vessels. C5b-9 diffusely expressed in vascular endothelial cells and smooth muscle layer. But the intensity was not related with the elevated level of serum CK. So, C5b-9 is strongly expressed in the necrotic muscle fiber and blood vessels, and may contribute to the pathogenesis of NM.
authors.affiliation: Department of Neurology, The Chinese PLA General Hospital Beijing, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Cong
authors.affiliation: Department of Neurology, The Chinese PLA General Hospital Beijing, China.
authors.forename: Chuan-Qiang
authors.initials: CQ
authors.lastname: Pu
authors.affiliation: Department of Neurology, The Chinese PLA General Hospital Beijing, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Department of Neurology, The Chinese PLA General Hospital Beijing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Neurology, The Chinese PLA General Hospital Beijing, China.
authors.forename: Xiang-Hui
authors.initials: XH
authors.lastname: Lu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D015938:Complement Membrane Attack Complex / Q000032:analysis / Q000276:immunology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000276:immunology / Q000473:pathology*; D009135:Muscular Diseases / Q000276:immunology* / Q000473:pathology*; D009336:Necrosis; D055815:Young Adult
chemical_list: D015938:Complement Membrane Attack Complex
keywords: C5b-9; Necrotizing myopathy; complement membrane attack complex; endothelial cell; muscle fiber