title: Crystal structure of a complex of NOD1 CARD and ubiquitin.
abstract: The Caspase Recruitment Domain (CARD) from the innate immune receptor NOD1 was crystallized with Ubiquitin (Ub). NOD1 CARD was present as a helix-swapped homodimer similar to other structures of NOD1 CARD, and Ub monomers formed a homodimer similar in conformation to Lys48-linked di-Ub. The interaction between NOD1 CARD and Ub in the crystal was mediated by novel binding sites on each molecule. Comparisons of these sites to previously identified interaction surfaces on both molecules were made along with discussion of their potential functional significance.
authors.affiliation: Molecular Physiology and Biophysics, University of Iowa, Iowa City, Iowa, United States of America; Department of Biochemistry, University of Iowa, Iowa City, Iowa, United States of America.
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Ver Heul
authors.affiliation: Department of Biochemistry, University of Iowa, Iowa City, Iowa, United States of America; Carver College of Medicine Protein Crystallography Facility, Iowa City, Iowa, United States of America.
authors.forename: Lokesh
authors.initials: L
authors.lastname: Gakhar
authors.affiliation: Molecular Physiology and Biophysics, University of Iowa, Iowa City, Iowa, United States of America.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Piper
authors.affiliation: Department of Biochemistry, University of Iowa, Iowa City, Iowa, United States of America; Institute for Stem Cell Biology and Regenerative Medicine, Bangalore, India.
authors.forename: Ramaswamy
authors.initials: R
authors.lastname: Subramanian
mesh_terms: D001665:Binding Sites; D006801:Humans; D008958:Models, Molecular; D053474:Nod1 Signaling Adaptor Protein / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D025801:Ubiquitin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D053474:Nod1 Signaling Adaptor Protein; D021122:Protein Subunits; D025801:Ubiquitin