title: Analysis of differential miRNA expression in primary tumor and stroma of colorectal cancer patients.
abstract: Microarray technology was used to profile miRNA expression in primary tumor and stromal tissue from paraffin embedded material of 51 patients with colorectal cancer. 26 miRNAs resulted differentially expressed with at least 2-fold change in tumor tissue with respect to stroma (16 more expressed in the tumor and 10 more expressed in the stroma). 10/26 were confirmed as differentially expressed at qRTPCR: miR-200c-3p, miR-141-3p, miR-200b-3p, miR-200a-3p, miR-1246, miR-92a-3p, miR-194-5p, miR-192-5p, miR-3651-5p, and miR-574-3p. No significant association was found between miRNA expressions and stage at diagnosis, site of primary tumor, first site of metastasis, progression-free, or overall survival.
authors.affiliation: Department of Clinical Medicine and Surgery, University Federico II, Via Sergio Pansini 5, 80131 Napoli, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Della Vittoria Scarpati
authors.affiliation: Department of Biology, University of Padova, Via 8 Febbraio 2, 35122 Padova, Italy.
authors.forename: Enrica
authors.identifier: 0000-0001-8463-2432
authors.initials: E
authors.lastname: Calura
authors.affiliation: Department of Oncology, Istituto di Ricerche Farmacologiche Mario Negri, Via La Masa 19, 20156 Milano, Italy.
authors.forename: Mariacristina
authors.identifier: 0000-0002-2889-5171
authors.initials: M
authors.lastname: Di Marino
authors.affiliation: Department of Biology, University of Padova, Via 8 Febbraio 2, 35122 Padova, Italy.
authors.forename: Chiara
authors.identifier: 0000-0003-4792-9047
authors.initials: C
authors.lastname: Romualdi
authors.affiliation: Department of Oncology, Istituto di Ricerche Farmacologiche Mario Negri, Via La Masa 19, 20156 Milano, Italy.
authors.forename: Luca
authors.identifier: 0000-0003-3631-6544
authors.initials: L
authors.lastname: Beltrame
authors.affiliation: Department of Public Health, University Federico II, Via Sergio Pansini 5, 80131 Napoli, Italy.
authors.forename: Umberto
authors.identifier: 0000-0003-3211-9957
authors.initials: U
authors.lastname: Malapelle
authors.affiliation: Department of Public Health, University Federico II, Via Sergio Pansini 5, 80131 Napoli, Italy.
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Troncone
authors.affiliation: Department of Clinical Medicine and Surgery, University Federico II, Via Sergio Pansini 5, 80131 Napoli, Italy.
authors.forename: Alfonso
authors.identifier: 0000-0003-2107-1177
authors.initials: A
authors.lastname: De Stefano
authors.affiliation: Department of Medicine, University of Salerno, Via Salvatore Allende 43, 84084 Baronissi, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Pepe
authors.affiliation: Department of Clinical Medicine and Surgery, University Federico II, Via Sergio Pansini 5, 80131 Napoli, Italy.
authors.forename: Sabino
authors.initials: S
authors.lastname: De Placido
authors.affiliation: Department of Oncology, Istituto di Ricerche Farmacologiche Mario Negri, Via La Masa 19, 20156 Milano, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: D'Incalci
authors.affiliation: Department of Oncology, Istituto di Ricerche Farmacologiche Mario Negri, Via La Masa 19, 20156 Milano, Italy.
authors.forename: Sergio
authors.identifier: 0000-0003-1783-7651
authors.initials: S
authors.lastname: Marchini
authors.affiliation: Department of Clinical Medicine and Surgery, University Federico II, Via Sergio Pansini 5, 80131 Napoli, Italy.
authors.forename: Chiara
authors.identifier: 0000-0001-5295-3004
authors.initials: C
authors.lastname: Carlomagno
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016000:Cluster Analysis; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs; D008875:Middle Aged; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology
chemical_list: D035683:MicroRNAs