title: Circulating Endothelial Cells and Platelet Microparticles in Mitral Valve Disease With and Without Atrial Fibrillation.
abstract: Hypercoagulability in mitral valve disease (MVD), a cause of atrial fibrillation (AF) and stroke, is potentially due to endothelial damage/dysfunction (marked by circulating endothelial cells [CECs]), platelet activation (soluble P-selectin [sPsel], platelet microparticles [PMPs], and soluble CD40 [sCD40]), and oxidized low-density lipoprotein (oxLDL) cholesterol. We measured these variables in 24 patients with MVD as well as in 21 with MVD + AF and compared them with 20 healthy controls (HCs). The CECs and PMPs were measured by flow cytometry; sPsel, oxLDL, and CD40 by enzyme-linked immunosorbent assay. Compared with HCs, sPsel and PMPs were equally higher in MVD and MVD + AF; sCD40 and oxLDL were higher in MVD + AF than in HCs and MVD; and CECs were higher in MVD than in the HCs, with further increases in MVD + AF (all P < .001). We conclude that excess platelet activation is present in MVD regardless of AF, and that increased endothelial damage in MVD is greater when compounded by AF.
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Assiut University, Assiut, Egypt.
authors.forename: Naglaa K
authors.initials: NK
authors.lastname: Idriss
authors.affiliation: Department of Medicine City Hospital, University of Birmingham Centre for Cardiovascular Sciences, Birmingham, United Kingdom a.blann@bham.ac.uk.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Blann
authors.affiliation: Department of Clinical Pathology, Faculty of Medicine, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.
authors.forename: Douaa M
authors.initials: DM
authors.lastname: Sayed
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Assiut University, Assiut, Egypt.
authors.forename: Marwa A
authors.initials: MA
authors.lastname: Gaber
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Assiut University, Assiut, Egypt.
authors.forename: Hosny A
authors.initials: HA
authors.lastname: Hassen
authors.affiliation: Department of Cardiology, Faculty of Medicine, Assiut University, Assiut, Egypt.
authors.forename: Yehia Taha
authors.initials: YT
authors.lastname: Kishk
mesh_terms: D000328:Adult; D001281:Atrial Fibrillation / Q000097:blood* / Q000209:etiology* / Q000503:physiopathology; D015415:Biomarkers / Q000097:blood; D001792:Blood Platelets / Q000473:pathology*; D023201:CD40 Ligand / Q000097:blood; D016022:Case-Control Studies; D042783:Endothelial Cells / Q000473:pathology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D008944:Mitral Valve Insufficiency / Q000097:blood* / Q000150:complications* / Q000503:physiopathology; D019007:P-Selectin / Q000097:blood; D015539:Platelet Activation; D011446:Prospective Studies; D012307:Risk Factors
chemical_list: D015415:Biomarkers; D008077:Lipoproteins, LDL; D019007:P-Selectin; C099252:oxidized low density lipoprotein; D023201:CD40 Ligand
keywords: atrial fibrillation; mitral valve disease; vascular damage