title: Protoplast fusion enhances lignocellulolytic enzyme activities in Trichoderma reesei.
abstract: Protoplast fusion was used to obtain a higher production of lignocellulolytic enzymes with protoplast fusion in Trichoderma reesei. The fusant strain T. reesei JL6 was obtained from protoplast fusion from T. reesei strains QM9414, MCG77, and Rut C-30. Filter paper activity of T. reesei JL6 increased by 18% compared with that of Rut C-30. β-Glucosidase, hemicellulase and pectinase activities of T. reesei JL6 were also higher. The former activity was 0.39 Uml(-1), while those of QM9414, MCG77, and Rut C-30 were 0.13, 0.11, and 0.16 Uml(-1), respectively. Pectinase and hemicellulase activities of JL6 were 5.4 and 15.6 Uml(-1), respectively, which were slightly higher than those of the parents. The effects of corn stover and wheat bran carbon sources on the cellulase production and growth curve of T. reesei JL6 were also investigated.
authors.affiliation: School of Life Science, Jilin University, Changchun, 130023, China.
authors.forename: Yu-xiao
authors.initials: YX
authors.lastname: Cui
authors.forename: Jia-jing
authors.initials: JJ
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Qi-yue
authors.initials: QY
authors.lastname: Cheng
authors.forename: Qun
authors.initials: Q
authors.lastname: Yu
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Ren
mesh_terms: D044602:Cellulases / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism*; D011523:Protoplasts / Q000201:enzymology*; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D014908:Triticum; D003313:Zea mays
chemical_list: C036909:lignocellulose; D008031:Lignin; D044602:Cellulases