title: Looking at proteins from two dimensions: a review on five decades of 2D electrophoresis.
abstract: Separating proteins according to two different gel-electrophoretic methods not only increases the resolution power for highly complex samples when compared to one-dimensional separations, but is also a valuable tool for protein and protein complex characterization. There are a number of different electrophoresis methods which can be combined. The combination of isoelectric focusing under denaturing conditions and SDS polyacrylamide gel electrophoresis delivers the highest resolution of all bio-analytic techniques. This is a short review on the history and state of the art of two-dimensional electrophoresis methods, and contains some practical tips for high resolution 2D electrophoresis, which are based on several decades of experience with this method.
authors.affiliation: Freising Province , Germany.
authors.forename: Reiner
authors.initials: R
authors.lastname: Westermeier
mesh_terms: D053000:Analytic Sample Preparation Methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
chemical_list: D011506:Proteins
keywords: Isoelectric focusing; SDS polyacrylamide gel electrophoresis; protein separation methods; proteomics; two-dimensional electrophoresis