title: Nestin expression and glial response in the hippocampus of mice after trimethyltin treatment.
abstract: Nestin is a protein of embryonic intermediate filaments expressed by multipotent neural stem cells. In the present study, the nestin expression pattern in the mouse hippocampus 1, 2, 3, 4, and 8 days after treatment with trimethyltin (TMT) was examined to explore the possible role played by nestin in chemically induced hippocampal injury. TMT treatment (2.5mg/kg, intraperitoneally) selectively injured the dentate gyrus (DG) of the mouse hippocampus. The level of hippocampal mRNA encoding nestin increased significantly 2 and 3 days post-treatment and thereafter decreased (at 4 and 8 days post-treatment). The level of nestin protein significantly increased 2 - 4 days post-treatment, particularly in the injured region of the DG, and predominantly in glial fibrillary acidic protein-positive astrocytes in the hippocampal DG. Ki67-positive proliferating cells were increased following TMT treatment and co-localized with nestin-positive reactive astrocytes. Thus, we suggest that nestin contributes to remodeling of the chemically injured DG via glial scar formation and the alteration of neurogenesis.
authors.affiliation: College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Sueun
authors.initials: S
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju 500-757, Republic of Korea; Department of Physiology and Neuroscience Program, Michigan State University, MI 48824, USA.
authors.forename: Miyoung
authors.initials: M
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Jinwook
authors.initials: J
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Juhwan
authors.initials: J
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Yeonghoon
authors.initials: Y
authors.lastname: Son
authors.affiliation: College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Seungjoo
authors.initials: S
authors.lastname: Kwon
authors.affiliation: College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Jong-Choon
authors.initials: JC
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Seong Soo
authors.initials: SS
authors.lastname: Kang
authors.affiliation: Department of Physiology and Neuroscience Program, Michigan State University, MI 48824, USA.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: shint@jejunu.ac.kr.
authors.forename: Taekyun
authors.initials: T
authors.lastname: Shin
authors.affiliation: College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju 500-757, Republic of Korea. Electronic address: moonc@chonnam.ac.kr.
authors.forename: Changjong
authors.initials: C
authors.lastname: Moon
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D018891:Dentate Gyrus / Q000187:drug effects / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D064231:Nestin / Q000378:metabolism*; D012333:RNA, Messenger; D014298:Trimethyltin Compounds / Q000494:pharmacology*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D064231:Nestin; D012333:RNA, Messenger; D014298:Trimethyltin Compounds; C046488:trimethyltin
keywords: Astrocytes; Dentate gyrus; Hippocampus; Mice; Nestin; Trimethyltin