title: Analysis of rat testicular proteome following 30-day exposure to 900 MHz electromagnetic field radiation.
abstract: The use of electromagnetic field (EMF) generating apparatuses such as cell phones is increasing, and has caused an interest in the investigations of its effects on human health. We analyzed proteome in preparations from the whole testis in adult male Sprague-Dawley rats that were exposed to 900 MHz EMF radiation for 1, 2, or 4 h/day for 30 consecutive days, simulating a range of possible human cell phone use. Subjects were sacrificed immediately after the end of the experiment and testes fractions were solubilized and separated via high-resolution 2D electrophoresis, and gel patterns were scanned, digitized, and processed. Thirteen proteins, which were found only in sham or in exposure groups, were identified by MALDI-TOF/TOF-MS. Among them, heat shock proteins, superoxide dismutase, peroxiredoxin-1, and other proteins related to misfolding of proteins and/or stress were identified. These results demonstrate significant effects of radio frequency modulated EMFs exposure on proteome, particularly in protein species in the rodent testis, and suggest that a 30-day exposure to EMF radiation induces nonthermal stress in testicular tissue. The functional implication of the identified proteins was discussed.
authors.affiliation: Department of Biochemistry, School of Veterinary Medicine, Shiraz University, Shiraz, Iran; Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Masood
authors.initials: M
authors.lastname: Sepehrimanesh
authors.forename: Nasrin
authors.initials: N
authors.lastname: Kazemipour
authors.forename: Mehdi
authors.initials: M
authors.lastname: Saeb
authors.forename: Saeed
authors.initials: S
authors.lastname: Nazifi
mesh_terms: D000818:Animals; D004574:Electromagnetic Fields; D015180:Electrophoresis, Gel, Two-Dimensional; D006360:Heat-Shock Proteins / Q000032:analysis / Q000737:chemistry; D008297:Male; D054464:Peroxiredoxins / Q000032:analysis / Q000737:chemistry; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000528:radiation effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000032:analysis / Q000737:chemistry; D013737:Testis / Q000737:chemistry / Q000528:radiation effects*
chemical_list: D006360:Heat-Shock Proteins; D020543:Proteome; D054464:Peroxiredoxins; D013482:Superoxide Dismutase
keywords: Heat shock proteins; Peroxiredoxin-1; Proteomic; Radio frequency modulated electromagnetic field; Superoxide dismutase