title: Rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay.
abstract: White spot syndrome virus (WSSV) causes large economic losses to the shrimp aquaculture industry, and thus far there are no efficient therapeutic treatments available against this lethal virus. In this study, we present the development of a novel real time isothermal recombinase polymerase amplification (RPA) assay for WSSV detection on a small ESEQuant Tube Scanner device. The RPA sensitivity, specificity and rapidity were evaluated by using a plasmid standard as well as viral and shrimp genomic DNAs. Compared with qPCR, the RPA assay revealed more satisfactory performance. It reached a detection limit up to 10 molecules in 95% of cases as determined by probit analysis of 8 independent experiments within 6.41 ± 0.17 min at 39 °C. Consequently, this rapid RPA method has great application potential for field use or point of care diagnostics.
authors.affiliation: Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage & Preservation, Ministry of Agriculture, Shanghai, China; Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai, China; College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Xia
authors.affiliation: Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage & Preservation, Ministry of Agriculture, Shanghai, China; Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai, China; College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Institute of Aquaculture, University of Stirling, Stirling, United Kingdom.
authors.forename: Manfred
authors.initials: M
authors.lastname: Weidmann
authors.affiliation: Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage & Preservation, Ministry of Agriculture, Shanghai, China; Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai, China; College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage & Preservation, Ministry of Agriculture, Shanghai, China; Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai, China; College of Food Science and Technology, Shanghai Ocean University, Shanghai, China; Institute of Biochemistry and Molecular Cell Biology, University of Göttingen, Göttingen, Germany.
authors.forename: Shuling
authors.initials: S
authors.lastname: Yan
authors.affiliation: Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage & Preservation, Ministry of Agriculture, Shanghai, China; Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai, China; College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D003445:Crustacea / Q000821:virology; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D057230:Limit of Detection; D010957:Plasmids; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D046848:White spot syndrome virus 1 / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral