title: The peroxidase/catalase-like activities of MFe₂O₄ (M=Mg, Ni, Cu) MNPs and their application in colorimetric biosensing of glucose.
abstract: MFe2O4 (M=Mg, Ni, Cu) magnetic nanoparticles (MNPs) were found to have catalytic activities similar to those of biological enzymes such as catalase and peroxidase. These nanomaterials, as bifunctional catalase/peroxidases (KatGs), not only could catalyze H2O2 to produce hydroxyl radicals, which oxidized peroxidase substrate to produce color, but also could catalyze the decomposition reaction of H2O2 into water and oxygen directly in the same condition through the catalase-like activity. And it was also found that the amount of generated hydroxyl radicals and oxygen was related to the concentration of MFe2O4 (M=Mg, Ni, Cu) MNPs. The peroxidase-like catalytic behavior of MFe2O4 MNPs was analyzed in detail. Under the optimized conditions, NiFe2O4 MNPs were used as a colorimetric biosensor for the detection of 9.4×10(-7)-2.5×10(-5) mol L(-1) glucose with a limit of detection (LOD) of 4.5×10(-7) mol L(-1). The sensor was successfully applied to glucose detection in urine sample.
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; School of Chemistry and Chemistry Engineering, Henan Normal University, Xinxiang, Henan 453007, China.
authors.forename: Li
authors.initials: L
authors.lastname: Su
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Huige
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Zia Ur
authors.initials: ZU
authors.lastname: Rahman
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Cuiling
authors.initials: C
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Sudai
authors.initials: S
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China. Electronic address: chenxg@lzu.edu.cn.
authors.forename: Xingguo
authors.initials: X
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002374:Catalase / Q000737:chemistry; D003124:Colorimetry; D005947:Glucose / Q000737:chemistry / Q000302:isolation & purification*; D006029:Glycosuria / Q000175:diagnosis; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D009195:Peroxidase / Q000737:chemistry
chemical_list: D058185:Magnetite Nanoparticles; D006861:Hydrogen Peroxide; D002374:Catalase; D009195:Peroxidase; D005947:Glucose
keywords: Colorimetric biosensing; Glucose detection; MFe(2)O(4) magnetic nanoparticles; Peroxidase/catalase mimetic