title: Migration and differentiation of gonadal germ cells under cross-sex germline chimeras condition in domestic chickens.
abstract: A series of experiments was conducted to investigate migration, proliferation and differentiation of gonadal germ cells (GGCs) collected from the gonads of 7-day-old chick embryos under cross-sex germline chimera conditions. The migratory and proliferative abilities of exogenous GGCs were examined by transferring 50 fluorescently labeled GGCs collected from White Leghorn (WL) embryos into the blood of 2-day-old Rhode Island Red (RIR) embryos. No significant difference was observed in the number of fluorescently labeled GGCs in the gonads of recipient embryos among any of the four possible donor and recipient sex combinations. Cross-sex germline chimeras were produced to examine the differentiation of GGCs by transferring 100 GGCs from WL embryos into 2-day-old RIR embryos. Exogenous-GGC-derived progeny were obtained from both male and female recipients, except when female GGCs were transferred into male recipients. The migratory ability of GGCs recovered from the 7-day-old embryonic gonad was not influenced by cross-sex germ cell transfer conditions, whereas the differentiation of the GGCs was affected by the sex combinations of GGCs donors and recipients.
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8572, Japan; Fellow of the Japanese Society for the Promotion of Science.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nakajima
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Hattori
authors.forename: Atsushi
authors.initials: A
authors.lastname: Asano
authors.forename: Naoto
authors.initials: N
authors.lastname: Ishikawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Tajima
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology; D002645:Chickens; D002678:Chimera / Q000502:physiology; D005260:Female; D005854:Germ Cells / Q000502:physiology*; D006066:Gonads / Q000166:cytology*; D008297:Male