title: Characterization of a multilayer heparin coating for biomolecule presentation to human mesenchymal stem cell spheroids.
abstract: Mesenchymal stem cells therapies have the potential to treat many pathologies, however, controlling cell fate after implantation remains challenging. We have used a multilayer technology to graft a range of 5 μg/mL - 5 mg/mL heparin onto the surface of MSC aggregates. Heparin coating does not affect cell viability (seen through LIVE/DEAD staining), cell anti-inflammatory properties (seen through co-culture with activated monocytes)and facilitates sequestration by coated cells of a growth factor (TGF-β1) that remains bioactive. This system can maximize therapeutic potential of MSC-based treatments because the cell surface-loaded protein could both signal to the cells to influence transplanted cell fate and be released into the surrounding environment to help repair injured tissue.
authors.affiliation: Woodruff School of Mechanical Engineering. Georgia Institute of Technology, Atlanta, Georgia, USA.
authors.forename: J
authors.initials: J
authors.lastname: Lei
authors.affiliation: School of Physics. Georgia Institute of Technology, Atlanta, Georgia, USA.
authors.forename: L T
authors.initials: LT
authors.lastname: McLane
authors.affiliation: School of Physics. Georgia Institute of Technology, Atlanta, Georgia, USA ; Wallace H. Coulter Department of Biomedical Engineering. Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Curtis
authors.affiliation: Wallace H. Coulter Department of Biomedical Engineering. Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA ; Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Temenoff