title: Rapid detection of Mycobacterium tuberculosis by recombinase polymerase amplification.
abstract: Improved access to effective tests for diagnosing tuberculosis (TB) has been designated a public health priority by the World Health Organisation. In high burden TB countries nucleic acid based TB tests have been restricted to centralised laboratories and specialised research settings. Requirements such as a constant electrical supply, air conditioning and skilled, computer literate operators prevent implementation of such tests in many settings. Isothermal DNA amplification technologies permit the use of simpler, less energy intensive detection platforms more suited to low resource settings that allow the accurate diagnosis of a disease within a short timeframe. Recombinase Polymerase Amplification (RPA) is a rapid, low temperature isothermal DNA amplification reaction. We report here RPA-based detection of Mycobacterium tuberculosis complex (MTC) DNA in <20 minutes at 39 °C. Assays for two MTC specific targets were investigated, IS6110 and IS1081. When testing purified MTC genomic DNA, limits of detection of 6.25 fg (IS6110) and 20 fg (IS1081)were consistently achieved. When testing a convenience sample of pulmonary specimens from suspected TB patients, RPA demonstrated superior accuracy to indirect fluorescence microscopy. Compared to culture, sensitivities for the IS1081 RPA and microscopy were 91.4% (95%CI: 85, 97.9) and 86.1% (95%CI: 78.1, 94.1) respectively (n = 71). Specificities were 100% and 88.6% (95% CI: 80.8, 96.1) respectively. For the IS6110 RPA and microscopy sensitivities of 87.5% (95%CI: 81.7, 93.2) and 70.8% (95%CI: 62.9, 78.7) were obtained (n = 90). Specificities were 95.4 (95% CI: 92.3,98.1) and 88% (95% CI: 83.6, 92.4) respectively. The superior specificity of RPA for detecting tuberculosis was due to the reduced ability of fluorescence microscopy to distinguish Mtb complex from other acid fast bacteria. The rapid nature of the RPA assay and its low energy requirement compared to other amplification technologies suggest RPA-based TB assays could be of use for integration into a point-of-care test for use in resource constrained settings.
authors.affiliation: Program for Appropriate Technology in Health, Seattle, WA, United States of America.
authors.forename: David S
authors.initials: DS
authors.lastname: Boyle
authors.affiliation: Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom.
authors.forename: Ruth
authors.initials: R
authors.lastname: McNerney
authors.affiliation: Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom.
authors.forename: Hwee
authors.initials: H
authors.lastname: Teng Low
authors.affiliation: Program for Appropriate Technology in Health, Seattle, WA, United States of America.
authors.forename: Brandon Troy
authors.initials: BT
authors.lastname: Leader
authors.affiliation: Washington State Department of Health, Public Health Laboratories, Shoreline, WA, United States of America.
authors.forename: Ailyn C
authors.initials: AC
authors.lastname: Pérez-Osorio
authors.affiliation: TwistDx Limited, Cambridge, United Kingdom.
authors.forename: Jessica C
authors.initials: JC
authors.lastname: Meyer
authors.affiliation: Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom.
authors.forename: Denise M
authors.initials: DM
authors.lastname: O'Sullivan
authors.affiliation: TwistDx Limited, Cambridge, United Kingdom.
authors.forename: David G
authors.initials: DG
authors.lastname: Brooks
authors.affiliation: TwistDx Limited, Cambridge, United Kingdom.
authors.forename: Olaf
authors.initials: O
authors.lastname: Piepenburg
authors.affiliation: TwistDx Limited, Cambridge, United Kingdom.
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Forrest
mesh_terms: D004269:DNA, Bacterial / Q000737:chemistry; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis*
chemical_list: D004269:DNA, Bacterial