title: Supported molecular matrix electrophoresis: a new membrane electrophoresis for characterizing glycoproteins.
abstract: Protein blotting is often used for identification and characterization of proteins on a membrane to which proteins separated by gel electrophoresis are transferred. The transferring process is sometimes problematic, in particular, for mucins and proteoglycans. Here, we describe a novel membrane electrophoresis technique, termed supported molecular matrix electrophoresis (SMME), in which a porous polyvinylidene difluoride (PVDF) membrane filter is used as the separation support. Proteins separated by this method can be immunoblotted without any transferring procedures.
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central-2, 1-1-1, Umezono, Tsukuba, Ibaraki, 305-8568, Japan.
authors.forename: Yu-ki
authors.initials: YK
authors.lastname: Matsuno
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kameyama
mesh_terms: D004586:Electrophoresis / Q000379:methods*; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D006358:Hot Temperature; D007150:Immunohistochemistry; D008567:Membranes, Artificial; D011145:Polyvinyls / Q000737:chemistry; D005465:Fluorocarbon Polymers
chemical_list: D006023:Glycoproteins; D008567:Membranes, Artificial; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers