title: Long-term globular adiponectin administration improves adipose tissue dysmetabolism in high-fat diet-fed Wistar rats.
abstract: Adiponectin administration to obese or type 2 diabetic patients is still far off, due to its expensive costs and absence of studies demonstrating the effectiveness of its chronic administration. We performed long-term globular adiponectin administration, testing its usefulness in improving adipose tissue metabolism. Adiponectin (98 υg/day) was administered through a subcutaneous minipump with continued release (28 days) to Wistar rats fed a high-fat diet. Adiponectin decreased body weight and adipocyte size, while decreasing circulating leptin levels. More, adiponectin was able to increase IkappaBalpha and PPARgamma levels and to prevent high-fat diet-induced impairment of insulin signalling, especially in epididymal adipose tissue. This resulted in improved glucose profile. High-fat diet caused an impairment of lipolysis in epididymal adipose tissue, which was partially restored by adiponectin treatment. Long-term globular adiponectin administration was able to improve pathways of insulin signalling and lipid storage in adipose tissue of high-fat diet-fed rats, contributing to a better metabolic profile.
authors.affiliation: Laboratory of Physiology, Institute of Biomedical Imaging and Life Sciences (IBILI), Faculty of Medicine, University of Coimbra , Portugal .
authors.forename: P
authors.initials: P
authors.lastname: Matafome
authors.forename: T
authors.initials: T
authors.lastname: Rodrigues
authors.forename: A
authors.initials: A
authors.lastname: Pereira
authors.forename: L
authors.initials: L
authors.lastname: Letra
authors.forename: H
authors.initials: H
authors.lastname: Azevedo
authors.forename: A
authors.initials: A
authors.lastname: Paixão
authors.forename: M
authors.initials: M
authors.lastname: Silvério
authors.forename: A
authors.initials: A
authors.lastname: Almeida
authors.forename: C
authors.initials: C
authors.lastname: Sena
authors.forename: R
authors.initials: R
authors.lastname: Seiça
mesh_terms: D052242:Adiponectin / Q000008:administration & dosage*; D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D059305:Diet, High-Fat; D018149:Glucose Intolerance / Q000517:prevention & control*; D006984:Hypertrophy / Q000517:prevention & control; D007328:Insulin / Q000378:metabolism*; D008066:Lipolysis / Q000187:drug effects; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D017208:Rats, Wistar; D015430:Weight Gain / Q000187:drug effects
chemical_list: D052242:Adiponectin; D007328:Insulin
keywords: Adipose tissue; insulin signalling; recombinant adiponectin administration; systemic dysmetabolism