title: Analysis of volatile organic compounds liberated and metabolised by human umbilical vein endothelial cells (HUVEC) in vitro.
abstract: Gas chromatography with mass spectrometric detection combined with head-space needle trap extraction as the pre-concentration technique was applied to identify and quantify volatile organic compounds released or metabolised by human umbilical vein endothelial cells. Amongst the consumed species there were eight aldehydes (2-methyl 2-propenal, 2-methyl propanal, 2-methyl butanal, 3-methyl butanal, n-hexanal, benzaldehyde, n-octanal and n-nonanal) and n-butyl acetate. Further eight compounds (ethyl acetate, ethyl propanoate, ethyl butyrate, 3-heptanone, 2-octanone, 2-nonanone, 2-methyl-5-(methylthio)-furan and toluene) were found to be emitted by the cells under study. Possible metabolic pathways leading to the uptake and release of these compounds by HUVEC are proposed and discussed. The uptake of aldehydes by endothelial cells questions the reliability of species from this chemical class as breath or blood markers of disease processes in human organism. The analysis of volatiles released or emitted by cell lines is shown to have a potential for the identification and assessment of enzymes activities and expression.
authors.affiliation: Breath Research Institute, University of Innsbruck, Rathausplatz 4, 6850, Dornbirn, Austria, Pawel.Mochalski@uibk.ac.at.
authors.forename: Paweł
authors.initials: P
authors.lastname: Mochalski
authors.forename: Markus
authors.initials: M
authors.lastname: Theurl
authors.forename: Andreas
authors.initials: A
authors.lastname: Sponring
authors.forename: Karl
authors.initials: K
authors.lastname: Unterkofler
authors.forename: Rudolf
authors.initials: R
authors.lastname: Kirchmair
authors.forename: Anton
authors.initials: A
authors.lastname: Amann
mesh_terms: D053000:Analytic Sample Preparation Methods; D001692:Biological Transport; D008401:Gas Chromatography-Mass Spectrometry; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism*; D006801:Humans; D055549:Volatile Organic Compounds / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D055549:Volatile Organic Compounds