title: [Anti-inflammatory activity of olive seed polyphenolic extract in the THP1-XBLUE-CD14 human monocytes cell line].
abstract: The aim of this study was to assess the anti-inflammatory activity of a polyphenolic extract from olive pits.
  
  
  METHODS
  The THP1-XBlue-CD14 (invivogen) cellular line, 80,000 cells/well, was incubated and inflammation (activation of NF-kb) was produced with 0.1 mg/mL of LPS (lipopolysaccharide from E. coli) for 24 hours. We assessed the presence of the extract (10 and 50 mg/L, biologically safe concentrations) for 2 hours at 37º C, before (preventive effect) and after (therapeutic effect) the proinflammatory activation, and the activity of alkaline phosphatase, which is expressed under the control of the NF-kb transcriptional factor, was quantified by colorimetry. The percentage of activity of NF-kb as preventive effect and therapeutic effect was assessed by comparing it to control cultures of cells with LPS and without extract, which are considered 100% of NF-kb.
  
  
  RESULTS
  The preventive anti-inflammatory capacity of the extract at 50 mg/L was 25.5% (95% CI: 16.8-34.2) and the therapeutic effect 34.9% (95% CI: 25.3-44.4) for the same concentration, without any significant activity at 10 mg/L.
  
  
  CONCLUSIONS
  An activity of polyphenols extracted from olive pits is shown, both in preventing inflammation and therapeutically eliminating inflammation through inhibition of NF-kB factor previously activated by LPS at concentrations of 50 mg/L of polyphenols, which previously haven been shown to be safe.
authors.affiliation: Departamento de Farmacología, Pediatría y Q. Orgánica. Universidad Miguel Hernández. Campus de San Juan. Alicante. España.. ernesto.cortes@umh.es.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Cortés Castell
authors.affiliation: Laboratorio de Biotecnología y Proyectos. Biopartner S.L.. cveciana@biopartner.net.
authors.forename: C
authors.initials: C
authors.lastname: Veciana Galindo
authors.affiliation: Laboratorio de Biotecnología y Proyectos. Biopartner S.L.. ltorro@biopartner.net.
authors.forename: L
authors.initials: L
authors.lastname: Torro Montell
authors.affiliation: Laboratorio de Biotecnología y Proyectos. Biopartner S.L.. esirvent@biopartner.net.
authors.forename: E
authors.initials: E
authors.lastname: Sirvent Segura
authors.affiliation: Departamento de Enfermería y Nutrición. Universidad de Alicante.. mercedes.rizo@ua.es.
authors.forename: M M
authors.initials: MM
authors.lastname: Rizo Baeza
authors.affiliation: Departamento de Medicina Clínica. Universidad Miguel Hernández. España.. vtgil@umh.es.
authors.forename: V
authors.initials: V
authors.lastname: Gil Guillén
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D006801:Humans; D009000:Monocytes / Q000187:drug effects*; D031658:Olea; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology*; D012639:Seeds
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D059808:Polyphenols