title: Development of highly sensitive electrochemical genosensor based on multiwalled carbon nanotubes-chitosan-bismuth and lead sulfide nanoparticles for the detection of pathogenic Aeromonas.
abstract: In this paper, we reported the construction of new high sensitive electrochemical genosensor based on multiwalled carbon nanotubes-chitosan-bismuth complex (MWCNT-Chi-Bi) and lead sulfide nanoparticles for the detection of pathogenic Aeromonas. Lead sulfide nanoparticles capped with 5'-(NH2) oligonucleotides thought amide bond was used as signalizing probe DNA (sz-DNA) and thiol-modified oligonucleotides sequence was used as fixing probe DNA (fDNA). The two probes hybridize with target Aeromonas DNA (tDNA) sequence (fDNA-tDNA-szDNA). The signal of hybridization is detected by differential pulse voltammetry (DPV) after electrodeposition of released lead nanoparticles (PbS) from sz-DNA on the surface of glass carbon electrode decorated with MWCNT-Chi-Bi, which improves the deposition and traducing electrical signal. The optimization of incubation time, hybridization temperature, deposition potential, deposition time and the specificity of the probes were investigated. Our results showed the highest sensibility to detect the target gene when compared with related biosensors and polymerase chain reaction (PCR). The detection limit for this biosensor was 1.0×10(-14) M. We could detect lower than 10(2) CFU mL(-1) of Aeromonas in spiked tap water. This method is rapid and sensitive for the detection of pathogenic bacteria and would become a potential application in biomedical diagnosis, food safety and environmental monitoring.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu 7214122, China. Electronic address: antmaxfer1@hotmail.com.
authors.forename: António Maximiano
authors.initials: AM
authors.lastname: Fernandes
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu 7214122, China.
authors.forename: Mandour H
authors.initials: MH
authors.lastname: Abdalhai
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu 7214122, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ji
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
authors.forename: Bing-Wen
authors.initials: BW
authors.lastname: Xi
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu 7214122, China.
authors.forename: Jiadi
authors.initials: J
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu 7214122, China.
authors.forename: Rasoamandrary
authors.initials: R
authors.lastname: Noeline
authors.affiliation: School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.
authors.forename: Byong H
authors.initials: BH
authors.lastname: Lee
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu 7214122, China. Electronic address: sxlzzz@jiangnan.edu.cn.
authors.forename: Xiulan
authors.initials: X
authors.lastname: Sun
mesh_terms: D000333:Aeromonas / Q000302:isolation & purification* / Q000472:pathogenicity; D015374:Biosensing Techniques / Q000379:methods*; D001729:Bismuth / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification*; D055664:Electrochemical Techniques / Q000379:methods*; D005516:Food Microbiology; D006801:Humans; D007854:Lead / Q000737:chemistry; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D013440:Sulfides / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D037742:Nanotubes, Carbon; D013440:Sulfides; C018391:lead sulfide; D007854:Lead; D048271:Chitosan; D001729:Bismuth
keywords: Aeromonas; Electrochemical biosensor; Foodborne pathogen; Lead sulfide nanoparticles