title: Simultaneous quantification of 5-hydroxyindoleacetic acid and 5-hydroxytryptamine by capillary electrophoresis with quantum dot and horseradish peroxidase enhanced chemiluminescence detection.
abstract: A capillary electrophoresis (CE) with chemiluminescence (CL) detection method was developed for the simultaneous quantification of 5-hydroxyindoleacetic acid (5-HIAA) and 5-hydroxytryptamine (5-HT). In this method, CdTe quantum dot (QD) and horseradish peroxidase (HRP) were used as enhancing reagents to co-catalyze the post-column CL reaction between luminol and hydrogen peroxide, achieving highly efficient CL emission. 5-HIAA and 5-HT inhibit the CL emission resulting to the formation of negative peaks in electropherogram. The degree of CL suppression is proportional to the concentration of 5-HT and 5-HIAA. The linear ranges for the determination of 5-HIAA and 5-HT were 2.5×10(-8)-2.5×10(-6) M and 2.5×10(-8)-5.0×10(-6) M with detection limits (signal/noise=3) of 7.0×10(-9) M and 6.0×10(-9) M, respectively. Intraday precision do not exceed 5.0%. The accuracy was confirmed by the recoveries ranged from 98% to 104%. The present method was successfully applied for the quantification of 5-HIAA and 5-HT in human urine. The concentrations of 5-HT and 5-HIAA in human urine were found to be in the range of 0.78-1.2 μM and 3.2-5.1 μM, respectively.
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Pharmacy, Guangxi Normal University, Guilin 541004, China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Pharmacy, Guangxi Normal University, Guilin 541004, China.
authors.forename: Yunsha
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Pharmacy, Guangxi Normal University, Guilin 541004, China.
authors.forename: Junming
authors.initials: J
authors.lastname: Huang
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Pharmacy, Guangxi Normal University, Guilin 541004, China. Electronic address: zhaoshulin001@163.com.
authors.forename: Shulin
authors.initials: S
authors.lastname: Zhao
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000652:urine*; D057230:Limit of Detection; D016014:Linear Models; D008163:Luminescent Measurements / Q000379:methods*; D045663:Quantum Dots; D015203:Reproducibility of Results; D012701:Serotonin / Q000652:urine*
chemical_list: D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D006735:Horseradish Peroxidase
keywords: 5-Hydroxyindoleacetic acid; 5-Hydroxytryptamine; Capillary electrophoresis; Chemiluminescence; Human urine; Quantum dot