title: Tracheal tissue engineering in rats.
abstract: Tissue-engineered tracheal transplants have been successfully performed clinically. However, before becoming a routine clinical procedure, further preclinical studies are necessary to determine the underlying mechanisms of in situ tissue regeneration. Here we describe a protocol using a tissue engineering strategy and orthotopic transplantation of either natural decellularized donor tracheae or artificial electrospun nanofiber scaffolds into a rat model. The protocol includes details regarding how to assess the scaffolds' biomechanical properties and cell viability before implantation. It is a reliable and reproducible model that can be used to investigate the crucial aspects and pathways of in situ tracheal tissue restoration and regeneration. The model can be established in <6 months, and it may also provide a means to investigate cell-surface interactions, cell differentiation and stem cell fate.
authors.affiliation: Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
authors.forename: Philipp
authors.initials: P
authors.lastname: Jungebluth
authors.affiliation: Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
authors.forename: Johannes C
authors.initials: JC
authors.lastname: Haag
authors.affiliation: Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Sjöqvist
authors.affiliation: Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
authors.forename: Ylva
authors.identifier: 0000000303930038
authors.initials: Y
authors.lastname: Gustafsson
authors.affiliation: Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
authors.forename: Antonio
authors.initials: A
authors.lastname: Beltrán Rodríguez
authors.affiliation: Department of Enterprise Engineering, University of Rome, Intrauniversity Consortium for Material Science and Technology (INSTM), Research Unit 'Tor Vergata', Rome, Italy.
authors.forename: Costantino
authors.initials: C
authors.lastname: Del Gaudio
authors.affiliation: Department of Enterprise Engineering, University of Rome, Intrauniversity Consortium for Material Science and Technology (INSTM), Research Unit 'Tor Vergata', Rome, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bianco
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Ivar
authors.initials: I
authors.lastname: Dehnisch
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Per
authors.initials: P
authors.lastname: Uhlén
authors.affiliation: Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
authors.forename: Silvia
authors.initials: S
authors.lastname: Baiguera
authors.affiliation: Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
authors.forename: Greg
authors.initials: G
authors.lastname: Lemon
authors.affiliation: Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
authors.forename: Mei Ling
authors.initials: ML
authors.lastname: Lim
authors.affiliation: Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose and Throat, Stockholm, Sweden.
authors.forename: Paolo
authors.initials: P
authors.lastname: Macchiarini
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D003124:Colorimetry; D048091:Guided Tissue Regeneration / Q000379:methods*; D057139:Nanofibers / Q000627:therapeutic use; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014132:Trachea / Q000502:physiology* / Q000637:transplantation