title: A novel eliminase from a marine bacterium that degrades hyaluronan and chondroitin sulfate.
abstract: Lyases cleave glycosaminoglycans (GAGs) in an eliminative mechanism and are important tools for the structural analysis and oligosaccharide preparation of GAGs. Various GAG lyases have been identified from terrestrial but not marine organisms even though marine animals are rich in GAGs with unique structures and functions. Herein we isolated a novel GAG lyase for the first time from the marine bacterium Vibrio sp. FC509 and then recombinantly expressed and characterized it. It showed strong lyase activity toward hyaluronan (HA) and chondroitin sulfate (CS) and was designated as HA and CS lyase (HCLase). It exhibited the highest activities to both substrates at pH 8.0 and 0.5 m NaCl at 30 °C. Its activity toward HA was less sensitive to pH than its CS lyase activity. As with most other marine enzymes, HCLase is a halophilic enzyme and very stable at temperatures from 0 to 40 °C for up to 24 h, but its activity is independent of divalent metal ions. The specific activity of HCLase against HA and CS reached a markedly high level of hundreds of thousands units/mg of protein under optimum conditions. The HCLase-resistant tetrasaccharide Δ(4,5)HexUAα1-3GalNAc(6-O-sulfate)β1-4GlcUA(2-O-sulfate)β1-3GalNAc(6-O-sulfate) was isolated from CS-D, the structure of which indicated that HCLase could not cleave the galactosaminidic linkage bound to 2-O-sulfated d-glucuronic acid (GlcUA) in CS chains. Site-directed mutagenesis indicated that HCLase may work via a catalytic mechanism in which Tyr-His acts as the Brønsted base and acid. Thus, the identification of HCLase provides a useful tool for HA- and CS-related research and applications.
authors.affiliation: From the National Glycoengineering Research Center, and State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China and.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Han
authors.affiliation: From the National Glycoengineering Research Center, and State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China and.
authors.forename: Wenshuang
authors.initials: W
authors.lastname: Wang
authors.affiliation: From the National Glycoengineering Research Center, and State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China and.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Proteoglycan Signaling and Therapeutics Research Group, Faculty of Advanced Life Science, Hokkaido University Graduate School of Life Science, Sapporo 001-0021, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Sugahara
authors.affiliation: From the National Glycoengineering Research Center, and State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China and fuchuanli@sdu.edu.cn.
authors.forename: Fuchuan
authors.initials: F
authors.lastname: Li
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002807:Chondroitin / Q000737:chemistry / Q000378:metabolism*; D002808:Chondroitin Lyases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004795:Enzyme Stability; D006820:Hyaluronic Acid / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012623:Seawater / Q000382:microbiology*; D013379:Substrate Specificity; D014733:Vibrio / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D006820:Hyaluronic Acid; D002807:Chondroitin; D002808:Chondroitin Lyases
keywords: Chondroitin Sulfate; Chondroitinase; Eliminase; Glycosaminoglycan; Hyaluronan; Hyaluronidase; Lyase; Marine Bacterium; Proteoglycan