title: Radixin enhances colon cancer cell invasion by increasing MMP-7 production via Rac1-ERK pathway.
abstract: As a member of the ezrin-radixin-moesin (ERM) family, radixin is overexpressed in many tumor tissues. However, little is known about its role in the progression of colon cancer. So we here aimed to determine the function of radixin in colon cancer cell invasion. Interestingly, we found that the expression of radixin was significantly elevated in colon cancer cells. Knockdown of radixin suppressed the invasion and migration of colon cancer cells. Further, knockdown of radixin inhibited the activation of Rac1 and ERK1/2, and decreased the expression and secretion of MMP-7. In addition, Rac1-ERK signaling pathway was required for the radixin-promoted invasion and MMP-7 production. Together, our findings suggest that radixin enhances the invasion and migration of colon cancer cells. Activation of Rac1-ERK pathway and consequent upregulation of MMP-7 production may contribute to the function of radixin in the regulation of colon cancer cell invasion. Thus, radixin may act as a novel target for the diagnosis and treatment of colon cancer.
authors.affiliation: Department of Gastroenterology, Pu'ai Hospital, Tongji Medical College, Huazhong University of Science and Technology, 50 Gu Tian San Road, Wuhan 430034, China.
authors.forename: Qi-Hong
authors.initials: QH
authors.lastname: Jiang
authors.affiliation: Department of Gastroenterology, Pu'ai Hospital, Tongji Medical College, Huazhong University of Science and Technology, 50 Gu Tian San Road, Wuhan 430034, China.
authors.forename: Ai-Xiang
authors.initials: AX
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Pu'ai Hospital, Tongji Medical College, Huazhong University of Science and Technology, 50 Gu Tian San Road, Wuhan 430034, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
mesh_terms: D015153:Blotting, Western; D018938:Caco-2 Cells; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D020935:MAP Kinase Signaling System / Q000235:genetics / Q000502:physiology*; D020783:Matrix Metalloproteinase 7 / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D020830:rac1 GTP-Binding Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; C107953:RAC1 protein, human; C071776:radixin; D020783:Matrix Metalloproteinase 7; D020830:rac1 GTP-Binding Protein