title: Interfacial and biological properties of the gradient coating on polyamide substrate for bone substitute.
abstract: Fabrication of bioactive and mechanical matched bone substitutes is crucial for clinical application in bone defects repair. In this study, nano-hydroxyapatite/polyamide (nHA/PA) composite was coated on injection-moulded PA by a chemical corrosion and phase-inversion technique. The shear strength, gradient composition and pore structure of the bioactive coating were characterized. Osteoblast-like MG63 cells were cultured on pure PA and composite-coated PA samples. The cells' adhesion, spread and proliferation were determined using MTT assay and microscopy. The results confirm that the samples with the nHA/PA composite coating have better cytocompatibility and have no negative effects on cells. To investigate the in vivo biocompatibility, both pure PA and composite-coated PA cylinders were implanted in the trochlea of rabbit femurs and studied histologically, and the bonding ability with bone were determined using push-out tests. The results show that composite-coated implants exhibit better biocompatibility and the shear strength of the composite-coated implants with host bone at 12 weeks can reach 3.49±0.42 MPa, which is significantly higher than that of pure PA implants. These results indicate that composite-coated PA implants have excellent biocompatibility and bonding abilities with host bone and they have the potential to be applied in repair of bone defects.
authors.affiliation: Department of Biomedical Engineering, Shanxi Key Laboratory of Material Strength and Structural Impact, College of Mechanics, Taiyuan University of Technology, Taiyuan 030024, People's Republic of China Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, People's Republic of China huangdi@tyut.edu.cn.
authors.forename: Di
authors.initials: D
authors.lastname: Huang
authors.affiliation: Department of Biomedical Engineering, Shanxi Key Laboratory of Material Strength and Structural Impact, College of Mechanics, Taiyuan University of Technology, Taiyuan 030024, People's Republic of China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Niu
authors.affiliation: Department of Biomedical Engineering, Shanxi Key Laboratory of Material Strength and Structural Impact, College of Mechanics, Taiyuan University of Technology, Taiyuan 030024, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Biomedical Engineering, Shanxi Key Laboratory of Material Strength and Structural Impact, College of Mechanics, Taiyuan University of Technology, Taiyuan 030024, People's Republic of China.
authors.forename: Meiqing
authors.initials: M
authors.lastname: Guo
authors.affiliation: Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, People's Republic of China zoae@vip.sina.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zuo
authors.affiliation: Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zou
authors.affiliation: Department of Biomedical Engineering, Shanxi Key Laboratory of Material Strength and Structural Impact, College of Mechanics, Taiyuan University of Technology, Taiyuan 030024, People's Republic of China.
authors.forename: Yinchun
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Biomedical Engineering, Shanxi Key Laboratory of Material Strength and Structural Impact, College of Mechanics, Taiyuan University of Technology, Taiyuan 030024, People's Republic of China.
authors.forename: Weiyi
authors.initials: W
authors.lastname: Chen
authors.affiliation: Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, People's Republic of China nic7504@scu.edu.cn.
authors.forename: Yubao
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology*; D017886:Durapatite / Q000737:chemistry*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry*; D010006:Osteoblasts / Q000737:chemistry*; D016062:Porosity; D011817:Rabbits; D033081:Shear Strength; D013052:Spectrometry, X-Ray Emission; D013499:Surface Properties; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D018786:Bone Substitutes; D009757:Nylons; D013778:Tetrazolium Salts; D013844:Thiazoles; D017886:Durapatite; C022616:thiazolyl blue
keywords: gradient coating; interfacial properties; nano-hydroxyapatite; osteogenesis; polyamide