title: Comparison of different DNA extraction methods from peripheral blood cells: advice from the Fondazione Italiana Linfomi Minimal Residual Disease Network.
abstract: Genomic DNA extraction is a primary component of genomic research and diagnostic routine analysis. Recently, the importance of this process has been highlighted by the necessity to standardize the diagnostic procedure. In this regard, the Minimal Residual Disease (MRD) Network of the Fondazione Italiana Linfomi (FIL MRD Network) has performed a comparative study of four different commercially available kits for DNA extraction, applying them on a panel of cellular pellets, with the aim of defining possible technical recommendations in order to harmonize and standardize diagnostic procedures in the clinical setting. Overall, all four kits usually allowed the recovery of a significant quantity of high-quality DNA (in most conditions), although specific indications could be addressed for cellular pellets of different sizes.
authors.affiliation: a Hematopathology Section, Department of Experimental , Diagnostic, and Specialty Medicine, S. Orsola-Malpighi Hospital, Bologna University , Bologna , Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Mannu
authors.affiliation: a Hematopathology Section, Department of Experimental , Diagnostic, and Specialty Medicine, S. Orsola-Malpighi Hospital, Bologna University , Bologna , Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Gazzola
authors.affiliation: b Division of Hematology, Department of Oncology , Santa Chiara Hospital , Pisa , Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Ciabatti
authors.affiliation: a Hematopathology Section, Department of Experimental , Diagnostic, and Specialty Medicine, S. Orsola-Malpighi Hospital, Bologna University , Bologna , Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Fuligni
authors.affiliation: c Hematology Section, Department of Cellular Biotechnologies and Hematology , "Sapienza" University of Rome , Rome , Italy.
authors.forename: Marzia
authors.initials: M
authors.lastname: Cavalli
authors.affiliation: c Hematology Section, Department of Cellular Biotechnologies and Hematology , "Sapienza" University of Rome , Rome , Italy.
authors.forename: Irene
authors.initials: I
authors.lastname: Della Starza
authors.affiliation: d University Division of Hematology, Hospital S. Giovanni Battista , Turin , Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Genuardi
authors.affiliation: d University Division of Hematology, Hospital S. Giovanni Battista , Turin , Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Mantoan
authors.affiliation: d University Division of Hematology, Hospital S. Giovanni Battista , Turin , Italy.
authors.forename: Luigia
authors.initials: L
authors.lastname: Monitillo
authors.affiliation: c Hematology Section, Department of Cellular Biotechnologies and Hematology , "Sapienza" University of Rome , Rome , Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Del Giudice
authors.affiliation: d University Division of Hematology, Hospital S. Giovanni Battista , Turin , Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Ladetto
authors.affiliation: e Division of Hematology, Department of Translational Medicine , Amedeo Avogadro University of Eastern Piedmont , Novara , Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Gaidano
authors.affiliation: a Hematopathology Section, Department of Experimental , Diagnostic, and Specialty Medicine, S. Orsola-Malpighi Hospital, Bologna University , Bologna , Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Sabattini
authors.affiliation: a Hematopathology Section, Department of Experimental , Diagnostic, and Specialty Medicine, S. Orsola-Malpighi Hospital, Bologna University , Bologna , Italy.
authors.forename: Stefano A
authors.initials: SA
authors.lastname: Pileri
authors.affiliation: b Division of Hematology, Department of Oncology , Santa Chiara Hospital , Pisa , Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Galimberti
authors.affiliation: a Hematopathology Section, Department of Experimental , Diagnostic, and Specialty Medicine, S. Orsola-Malpighi Hospital, Bologna University , Bologna , Italy.
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Piccaluga
keywords: DNA extraction; FIL MRD Network; PCR