title: Aneurinibacillus soli sp. nov., isolated from mountain soil.
abstract: A novel bacterial strain designated CB4(T) was isolated from soil from the Hallasan, Jeju, Korea. Strain CB4(T) was found to be strictly aerobic, Gram-stain-positive, rod-shaped, motile and formed creamy greyish colonies on nutrient agar. The major fatty acids were identified as iso-C(15:0) and iso-C(16:0), and the predominant isoprenoid quinone as MK-7. The cell-wall peptidoglycan contained glycine and alanine as the diagnostic amino acids and phosphatidyl-N-methylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol and an unidentified aminophospholipid as the polar lipids. The genomic DNA G+C content of strain CB4(T) was 46.5 mol%. Phylogenetic analysis, based on 16S rRNA gene sequence similarities, showed that strain CB4(T) forms a deep branch within the genus Aneurinibacillus, sharing the highest level of sequence homology with Aneurinibacillus aneurinilyticus DSM 5562(T) (96.5%). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain CB4(T) is considered to represent a novel species within the genus Aneurinibacillus, for which the name Aneurinibacillus soli sp. nov. is proposed. The type strain is CB4(T) ( =KCTC 33505(T) =CECT 8566(T)). An emended description of the genus Aneurinibacillus is also proposed.
authors.affiliation: Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Keun Chul
authors.initials: KC
authors.lastname: Lee
authors.affiliation: Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Kwang Kyu
authors.initials: KK
authors.lastname: Kim
authors.affiliation: Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Mi Kyung
authors.initials: MK
authors.lastname: Eom
authors.affiliation: Gyeongbuk Institute for Marine Bioindustry, Uljin 767-813, Republic of Korea.
authors.forename: Jong-Shik
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Research Institute for Hallasan, Jeju-si, Jeju Special Self-Governing Province 690-816, Republic of Korea.
authors.forename: Dae-Shin
authors.initials: DS
authors.lastname: Kim
authors.affiliation: Research Institute for Hallasan, Jeju-si, Jeju Special Self-Governing Province 690-816, Republic of Korea.
authors.forename: Suk-Hyung
authors.initials: SH
authors.lastname: Ko
authors.affiliation: Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Lee
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D016937:Gram-Positive Endospore-Forming Rods / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7