title: Desiccation enhances phosphorylation of PSII and affects the distribution of protein complexes in the thylakoid membrane.
abstract: Desiccation has significant effects on photosynthetic processes in intertidal macro-algae. We studied an intertidal macro-alga, Ulva sp., which can tolerate desiccation, to investigate changes in photosynthetic performance and the components and structure of thylakoid membrane proteins in response to desiccation. Our results demonstrate that photosystem II (PSII) is more sensitive to desiccation than photosystem I (PSI) in Ulva sp. Comparative proteomics of the thylakoid membrane proteins at different levels of desiccation suggested that there were few changes in the content of proteins involved in photosynthesis during desiccation. Interestingly, we found that both the PSII subunit, PsbS (Photosystem II S subunit) (a four-helix protein in the LHC superfamily), and light-harvesting complex stress-related (LHCSR) proteins, which are required for non-photochemical quenching in land plants and algae, respectively, were present under both normal and desiccation conditions and both increased slightly during desiccation. In addition, the results of immunoblot analysis suggested that the phosphorylation of PSII and LHCII increases during desiccation. To investigate further, we separated out a supercomplex formed during desiccation by blue native-polyacrylamide gel electrophoresis and identified the components by mass spectrometry analysis. Our results show that phosphorylation of the complex increases slightly with decreased water content. All the results suggest that during the course of desiccation, few changes occur in the content of thylakoid membrane proteins, but a rearrangement of the protein complex occurs in the intertidal macro-alga Ulva sp.
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Gao
authors.forename: Wenhui
authors.initials: W
authors.lastname: Gu
authors.forename: Qian
authors.initials: Q
authors.lastname: Xiong
authors.forename: Feng
authors.initials: F
authors.lastname: Ge
authors.forename: Xiujun
authors.initials: X
authors.lastname: Xie
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Weizhou
authors.initials: W
authors.lastname: Chen
authors.forename: Guanghua
authors.initials: G
authors.lastname: Pan
authors.forename: Guangce
authors.initials: G
authors.lastname: Wang
mesh_terms: D003890:Desiccation; D013058:Mass Spectrometry; D063347:Native Polyacrylamide Gel Electrophoresis; D010766:Phosphorylation; D010788:Photosynthesis; D045331:Photosystem I Protein Complex / Q000378:metabolism*; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D060431:Thylakoid Membrane Proteins / Q000378:metabolism*; D020524:Thylakoids / Q000378:metabolism; D044445:Ulva / Q000378:metabolism*
chemical_list: D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D060431:Thylakoid Membrane Proteins