title: A cytoprotective and degradable metal-polyphenol nanoshell for single-cell encapsulation.
abstract: Single-cell encapsulation promises the cytoprotection of the encased cells against lethal stressors, reminiscent of the sporulation process in nature. However, the development of a cytocompatible method for chemically mimicking the germination process (i.e., shell degradation on-demand) has been elusive, despite the shell degradation being pivotal for the practical use of functional cells as well as for single cell-based biology. We report that an artificial shell, composed of tannic acid (TA) and Fe(III) , on individual Saccharomyces cerevisiae controllably degrades on-demand, while protecting the yeast from multiple external aggressors, including UV-C irradiation, lytic enzymes, and silver nanoparticles. Cell division is suppressed by the TA-Fe(III) shell, but restored fully upon shell degradation. The formation of a TA-Fe(III) shell would provide a versatile tool for achieving the chemical version of "sporulation and germination".
authors.affiliation: Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon 305-701 (Korea).
authors.forename: Ji Hun
authors.initials: JH
authors.lastname: Park
authors.forename: Kyunghwan
authors.initials: K
authors.lastname: Kim
authors.forename: Juno
authors.initials: J
authors.lastname: Lee
authors.forename: Ji Yu
authors.initials: JY
authors.lastname: Choi
authors.forename: Daewha
authors.initials: D
authors.lastname: Hong
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Yang
authors.forename: Frank
authors.initials: F
authors.lastname: Caruso
authors.forename: Younghoon
authors.initials: Y
authors.lastname: Lee
authors.forename: Insung S
authors.initials: IS
authors.lastname: Choi
mesh_terms: D018914:Cells, Immobilized / Q000166:cytology; D019610:Cytoprotection; D005290:Ferric Compounds / Q000737:chemistry*; D057145:Nanoshells / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects; D013634:Tannins / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; D059808:Polyphenols; D013634:Tannins
keywords: artificial spores; cytoprotection; encapsulation; organic-inorganic hybrid composites; tannic acid