title: Enhanced resistance of CXCR3 deficient mice to ocular HSV-1 infection is due to control of replication in the brain ependyma.
abstract: CXCR3 deficient (CXCR3(-/-)) mice are resistant to ocular HSV-1 infection in that less mice develop encephalitis and succumb to infection in comparison to wild type (WT) animals. A region of the brain previously identified to be crucial for development of encephalitis was evaluated in HSV-1-infected CXCR3(-/-) and WT mice. In this region, known as the ependyma, viral titer, infiltrating leukocyte populations, and key anti-viral cytokine message levels were evaluated. We found that CXCR3(-/-) mice possessed significantly less HSV-1 and expressed significantly more IFN-β mRNA in the brain ependyma compared to WT animals during the development of encephalitis.
authors.affiliation: Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
authors.forename: Chandra M
authors.initials: CM
authors.lastname: Kroll
authors.affiliation: Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA; Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA. Electronic address: dan-carr@ouhsc.edu.
authors.forename: Daniel J J
authors.initials: DJ
authors.lastname: Carr
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D001921:Brain / Q000473:pathology*; D004195:Disease Models, Animal; D004805:Ependyma / Q000378:metabolism* / Q000821:virology; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000235:genetics*; D006561:Herpes Simplex / Q000235:genetics* / Q000473:pathology*; D018259:Herpesvirus 1, Human; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D054367:Receptors, CXCR3 / Q000172:deficiency* / Q000235:genetics; D010948:Viral Plaque Assay
chemical_list: D015703:Antigens, CD; C515188:Cxcr3 protein, mouse; D000949:Histocompatibility Antigens Class II; D054367:Receptors, CXCR3
keywords: Brain ependyma; CXCR3; Herpes simplex virus type 1; Interferon-β