title: Chemokine-like factor 1 promotes the migration of rat primary cortical neurons by the induction of actin polymerization.
abstract: Chemokine-like factor 1 (CKLF1), a newly cloned chemotactic cytokine, plays an essential role in immune cell migration. However, the potential role of CKLF1 in the cortical neuronal migration remains unclear. In the present research, by measuring the distance between the margin of brain slices and the leading population of migrating cells, we showed that the extent of cell migration was markedly enhanced in response to CKLF1 treatment, which was significantly inhibited by the simultaneous addition of anti-CKLF1 antibody. By immunofluorescence staining, it was found that CKLF1 induced actin polymerization in primary cerebral cortical neurons. By wound-healing assays, it was found that CKLF1 stimulated the migration of cortical neurons in a dose-dependent manner. In conclusion, our data suggest that CKLF1 promotes the migration of rat primary cerebral cortical neurons by the induction of actin polymerization.
authors.affiliation: aState Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Neuroscience Center, Chinese Academy of Medical Sciences and Peking Union Medical College bDepartment of Anatomy, School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Wang
authors.forename: Yu-He
authors.initials: YH
authors.lastname: Yuan
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Wang
authors.forename: Shi-Feng
authors.initials: SF
authors.lastname: Chu
authors.forename: Ning
authors.initials: N
authors.lastname: Han
authors.forename: Nai-Hong
authors.initials: NH
authors.lastname: Chen
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000502:physiology*; D018925:Chemokines / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D062792:MARVEL Domain-Containing Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009474:Neurons / Q000502:physiology*; D058105:Polymerization; D017207:Rats, Sprague-Dawley; D046509:Tissue Culture Techniques; D014945:Wound Healing / Q000502:physiology
chemical_list: D000199:Actins; D018925:Chemokines; C486623:Cklf protein, rat; D062792:MARVEL Domain-Containing Proteins