title: Application of isoabsorption plots generated by high-performance liquid chromatography with diode array detection to the development of multicomponent quantitative analysis of traditional herbal medicines.
abstract: Multicomponent quantitative analysis is one of the mainstream quality control methods of traditional herbal medicines. Since the constituents of traditional herbal medicines samples are complex, the development of high-performance liquid chromatography methods is laborious. In this study, an isoabsorption plot, a chromatographic/spectrometric data image plotted by diode array detection was utilized to facilitate the establishment of a high-performance liquid chromatography method by optimizing and validating the detection conditions off-line. Consequently a simple, reliable and accurate method for simultaneous determination of seven active polyphenolic components (protocatechuic acid, chlorogenic acid, caffeic acid, p-coumaric acid, rosmarinic acid, scutellarin, and apigenin) in Qingfei mixture, a long-used Chinese prescription, was developed. The chromatographic separation was performed on a C18 column with gradient elution of phosphoric acid aqueous solution (0.05%, v/v) and acetonitrile, and a wavelength switch program optimized with isoplot was adopted for detection. The method was validated in terms of linearity, sensitivity, precision, repeatability, and accuracy and was successfully applied to the simultaneous determination of the seven polyphenolic components in different production batches of Qingfei Mixture. These results indicated that isoplot is an effective tool to improve the establishment of multicomponent quantitative analysis methods.
authors.affiliation: Affiliated Zhejiang Cancer Hospital of Zhejiang Chinese Medical University, Hangzhou, China; College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Luo
authors.initials: L
authors.lastname: Fang
authors.forename: Guonong
authors.initials: G
authors.lastname: Yang
authors.forename: Yu
authors.initials: Y
authors.lastname: Song
authors.forename: Fanzhu
authors.initials: F
authors.lastname: Li
authors.forename: Nengming
authors.initials: N
authors.lastname: Lin
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D047310:Apigenin / Q000032:analysis; D002109:Caffeic Acids / Q000032:analysis; D002138:Calibration; D002726:Chlorogenic Acid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000032:analysis; D003373:Coumaric Acids / Q000032:analysis; D053630:Depsides / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D005965:Glucuronates / Q000032:analysis; D062385:Hydroxybenzoates / Q000032:analysis; D057230:Limit of Detection; D010756:Phosphoric Acids / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D059808:Polyphenols / Q000737:chemistry*; D011422:Propionates; D015203:Reproducibility of Results; D000097123:Rosmarinic Acid
chemical_list: D000097:Acetonitriles; D047310:Apigenin; D002109:Caffeic Acids; D002726:Chlorogenic Acid; D002934:Cinnamates; D003373:Coumaric Acids; D053630:Depsides; D004365:Drugs, Chinese Herbal; D005965:Glucuronates; D062385:Hydroxybenzoates; D010756:Phosphoric Acids; D059808:Polyphenols; D011422:Propionates; D000097123:Rosmarinic Acid; C059183:qing-fei-tang; C484876:scutellarin; C009091:protocatechuic acid; C030242:phosphoric acid; C495469:p-coumaric acid; C040048:caffeic acid; C032159:acetonitrile
keywords: High-performance liquid chromatography; Isoabsorption plot; Polyphenolic compounds; Qingfei mixture; Traditional herbal medicine