title: Genetic characterization of porcine kobuvirus and detection of coinfecting pathogens in diarrheic pigs in Jiangsu Province, China.
abstract: In this study, 396 samples from diarrheic pigs on 46 pig farms in Jiangsu Province, China, were analyzed by RT-PCR. One-hundred eighty-one pigs from 37 farms tested positive for porcine kobuvirus (PKV). Phylogenetic analysis of the 3D gene from 19 isolates showed sequence homology of 88.0 %-100 % and 69.4 %-100 % for nucleotides and amino acids, respectively, while similarity to isolates of other kobuviruses was 69.6 %-78.8 % and 27.8 %-56.9 %, respectively. One-hundred eighty-five samples contained two or more pathogens, and 31/68 PKV-positive samples tested positive for other diarrheic pathogens, confirming the existence of PKV infection and coinfection.
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yang
authors.forename: Wenjie
authors.initials: W
authors.lastname: Jin
authors.forename: Zhenpeng
authors.initials: Z
authors.lastname: Zhao
authors.forename: Weidong
authors.initials: W
authors.lastname: Lin
authors.forename: Di
authors.initials: D
authors.lastname: Zhang
authors.forename: Enqi
authors.initials: E
authors.lastname: Yu
authors.forename: Aijian
authors.initials: A
authors.lastname: Qin
authors.forename: Hanchun
authors.initials: H
authors.lastname: Yang
mesh_terms: D000818:Animals; D002681:China; D016000:Cluster Analysis; D003967:Diarrhea / Q000662:veterinary* / Q000821:virology; D052699:Kobuvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000662:veterinary* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins