title: Two transaldolase isogenes from Moniliella megachiliensis behave in a different way depending on the stress class.
abstract: We cloned and sequenced two transaldolase genes from Moniliella megachiliensis, a microorganism known to produce a significant amount of erythritol under hyper-osmotic stress. The amino acid sequences encoded by these two genes (MmTAL1, MmTAL2) showed 72% homology to each other. An AP-1 (ap response element) associated with oxidative stress was found in the promoter region of MmTAL1, while four STREs (stress response element) associated with osmotic stress were found in the promoter region of MmTAL2. In early-stage cultivation (up to 2 h), MmTAL1 was specifically expressed in response to oxidative stress generated by the presence of 0.15 mM menadione; expression level 3-fold higher than before stress loading. MmTAL2 was expressed in response to osmotic stress caused by 1.2 M NaCl; expression level was 21-fold higher than stress-free control. Erythritol accumulated intracellularly under osmotic and oxidative stress, approximately 30-fold and 35-fold, respectively. We therefore concluded that M. megachiliensis selectively uses two isogenes and produces erythritol during early-stage response to stress, depending on the type of environmental stress.
authors.affiliation: Fermentation and Applied Microbiology Laboratory, Department of Chemistry and Life Science, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Iwata
authors.affiliation: Fermentation and Applied Microbiology Laboratory, Department of Chemistry and Life Science, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Daiki
authors.initials: D
authors.lastname: Mizushima
authors.affiliation: Fermentation and Applied Microbiology Laboratory, Department of Chemistry and Life Science, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Kobayashi
authors.affiliation: Biomolecular Engineering Laboratory, National Food Research Institute, 2-1-12, Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ookura
authors.affiliation: Fermentation and Applied Microbiology Laboratory, Department of Chemistry and Life Science, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Ogihara
authors.affiliation: Fermentation and Applied Microbiology Laboratory, Department of Chemistry and Life Science, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Kato
authors.affiliation: Fermentation and Applied Microbiology Laboratory, Department of Chemistry and Life Science, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan. Electronic address: kasumi@brs.nihon-u.ac.jp.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Kasumi
mesh_terms: D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000201:enzymology* / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004896:Erythritol / Q000096:biosynthesis; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D018384:Oxidative Stress / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D020218:Response Elements / Q000235:genetics; D014153:Transaldolase / Q000737:chemistry / Q000235:genetics*; D018808:Transcription Factor AP-1 / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D018808:Transcription Factor AP-1; D014153:Transaldolase; D004896:Erythritol
keywords: Moniliella megachiliensis; Osmotic stress; Oxidative stress; Polyol; Transaldolase isogene