title: Mass spectrometry based proteomic analysis of salivary glands of urban malaria vector Anopheles stephensi.
abstract: Salivary gland proteins of Anopheles mosquitoes offer attractive targets to understand interactions with sporozoites, blood feeding behavior, homeostasis, and immunological evaluation of malaria vectors and parasite interactions. To date limited studies have been carried out to elucidate salivary proteins of An. stephensi salivary glands. The aim of the present study was to provide detailed analytical attributives of functional salivary gland proteins of urban malaria vector An. stephensi. A proteomic approach combining one-dimensional electrophoresis (1DE), ion trap liquid chromatography mass spectrometry (LC/MS/MS), and computational bioinformatic analysis was adopted to provide the first direct insight into identification and functional characterization of known salivary proteins and novel salivary proteins of An. stephensi. Computational studies by online servers, namely, MASCOT and OMSSA algorithms, identified a total of 36 known salivary proteins and 123 novel proteins analysed by LC/MS/MS. This first report describes a baseline proteomic catalogue of 159 salivary proteins belonging to various categories of signal transduction, regulation of blood coagulation cascade, and various immune and energy pathways of An. stephensi sialotranscriptome by mass spectrometry. Our results may serve as basis to provide a putative functional role of proteins in concept of blood feeding, biting behavior, and other aspects of vector-parasite host interactions for parasite development in anopheline mosquitoes.
authors.affiliation: Protein Biochemistry and Structural Biology Laboratory, National Institute of Malaria Research (ICMR), Sector-8, Dwarka, New Delhi 110077, India.
authors.forename: Sonam
authors.initials: S
authors.lastname: Vijay
authors.affiliation: Protein Biochemistry and Structural Biology Laboratory, National Institute of Malaria Research (ICMR), Sector-8, Dwarka, New Delhi 110077, India ; Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, NM 87106, USA.
authors.forename: Manmeet
authors.identifier: 0000-0002-8325-3390
authors.initials: M
authors.lastname: Rawat
authors.affiliation: Protein Biochemistry and Structural Biology Laboratory, National Institute of Malaria Research (ICMR), Sector-8, Dwarka, New Delhi 110077, India.
authors.forename: Arun
authors.identifier: 0000-0002-0928-9926
authors.initials: A
authors.lastname: Sharma
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D006790:Host-Parasite Interactions / Q000235:genetics; D006801:Humans; D007303:Insect Vectors / Q000235:genetics*; D008288:Malaria / Q000235:genetics* / Q000473:pathology / Q000635:transmission; D013058:Mass Spectrometry; D040901:Proteomics; D012469:Salivary Glands; D012471:Salivary Proteins and Peptides / Q000096:biosynthesis; D053719:Tandem Mass Spectrometry
chemical_list: D012471:Salivary Proteins and Peptides