title: Incidence and dynamics of active cytomegalovirus infection in allogeneic stem cell transplant patients according to single nucleotide polymorphisms in donor and recipient CCR5, MCP-1, IL-10, and TLR9 genes.
abstract: Single nucleotide polymorphisms (SNPs) in genes involved in the activation or regulation of innate and adaptive immune responses may modulate the susceptibility to and the natural history of certain chronic viral infections. The current study aimed to investigate whether donor and recipient SNPs in the chemokine receptor 5 (rs1800023), monocyte chemoattractant protein 1 (rs13900), interleukin-10 (rs1878672), and Toll-like receptor 9 (rs352140) genes would exert any influence on the rate of incidence and features of CMV DNAemia in the allogeneic stem cell transplantation setting. This was a retrospective observational multicenter study. The cohort consisted of 102 non-consecutive allogeneic stem cell transplant recipients. SNP genotyping was performed by allele-specific real-time PCR. CMV surveillance was performed by the pp65 antigenemia assay/and or by real-time PCR. Seventy-three patients developed CMV DNAemia within the first 100 days after transplantation (71.5%). Neither donor nor recipient SNPs were associated significantly with the rate of incidence of active CMV infection, nor with the need for pre-emptive antiviral therapy. Both the duration of CMV DNAemia and the plasma CMV DNA peak load during episodes were significantly higher in patients harboring the donor (but not the recipient) chemokine receptor 5 A/A genotype, than in their A/G and G/G counterparts (P = 0.022 and P = 0.045, respectively). The data reported suggest that SNPs in chemokine receptor 5 may influence the dynamics of CMV infection in the Allo-SCT setting.
authors.affiliation: Microbiology Service, Hospital Clínico Universitario, Fundación INCLIVA, Valencia, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Corrales
authors.forename: Estela
authors.initials: E
authors.lastname: Giménez
authors.forename: Carlos
authors.initials: C
authors.lastname: Solano
authors.forename: Paula
authors.initials: P
authors.lastname: Amat
authors.forename: Rafael
authors.initials: R
authors.lastname: de la Cámara
authors.forename: José
authors.initials: J
authors.lastname: Nieto
authors.forename: Ana
authors.initials: A
authors.lastname: Garcia-Noblejas
authors.forename: David
authors.initials: D
authors.lastname: Navarro
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018932:Chemokine CCL2 / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000453:epidemiology* / Q000235:genetics; D004279:DNA, Viral / Q000097:blood; D005260:Female; D056726:Genetic Association Studies; D006801:Humans; D015994:Incidence; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D019713:Receptors, CCR5 / Q000235:genetics*; D012189:Retrospective Studies; D033581:Stem Cell Transplantation / Q000009:adverse effects*; D014019:Tissue Donors; D051217:Toll-Like Receptor 9 / Q000235:genetics*; D066027:Transplant Recipients; D014184:Transplantation, Homologous / Q000009:adverse effects*; D014766:Viremia / Q000453:epidemiology / Q000235:genetics; D055815:Young Adult
chemical_list: C111835:CCL2 protein, human; C584933:CCR5 protein, human; D018932:Chemokine CCL2; D004279:DNA, Viral; C508609:IL10 protein, human; D019713:Receptors, CCR5; C495371:TLR9 protein, human; D051217:Toll-Like Receptor 9; D016753:Interleukin-10
keywords: CMV DNAemia; Cytomegalovirus (CMV); Single nucleotide polymorphisms; allogeneic stem cell transplantation