title: In vitro properties of apheresis platelet during extended storage in plasma treated with anandamide.
abstract: BACKGROUND
  In China apheresis platelets (PLTs) are stored in plasma for only 5 days, resulting in PLT inventory pressures. Anandamide (ANA) was reported to be a potential agent to inhibit PLT apoptosis. The aim of this study was to evaluate the characteristics of extended storage PLTs in plasma treated with ANA in vitro.
  
  
  METHODS
  Apheresis PLTs (n = 20) were prepared in plasma treated with ANA, and stored at 22 °C for up to 11 days. On day 1, 3, 5, 7, 9 and 11, PLTs were tested for PLT count, mean PLT volume (MPV), PLT distribution width (PDW), pH, pCO(2), pO(2), hypotonic shock response (HSR), phosphatidylserine (PS) exposure and soluble P-selectin content.
  
  
  RESULTS
  PLTs stored in plasma with/without ANA didn't show significant differences during the first 5 days of storage. From the 7(th) day on, PLTs stored in plasma with ANA displayed significantly lower PS expression, soluble P-selectin content and higher HSR scores than those stored in plasma without ANA (P <0.05), respectively.
  
  
  CONCLUSIONS
  The extended storage of PLTs in plasma treated with 0.5 µmol/l ANA showed better characteristics of the PLTs, compared with the control group, which was suggested to potentially alleviate the PLT storage lesion.
authors.affiliation: Blood Center of Shandong Province, Jinan 250014, Shandong Province, China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: School of Medicine, Shandong University, Jinan 250012, Shandong Province, China. Electronic address: rengj@sdu.edu.cn.
authors.forename: Guijie
authors.initials: G
authors.lastname: Ren
authors.affiliation: Bio-technology Department, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Huiyu
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Medicine, Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Keli
authors.initials: K
authors.lastname: Tian
authors.affiliation: Blood Center of Shandong Province, Jinan 250014, Shandong Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Blood Center of Shandong Province, Jinan 250014, Shandong Province, China.
authors.forename: Wenben
authors.initials: W
authors.lastname: Qiao
authors.affiliation: Blood Center of Shandong Province, Jinan 250014, Shandong Province, China.
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Nie
authors.affiliation: Blood Center of Shandong Province, Jinan 250014, Shandong Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Blood Center of Shandong Province, Jinan 250014, Shandong Province, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Song
authors.affiliation: Blood Center of Shandong Province, Jinan 250014, Shandong Province, China.
authors.forename: Chuanfu
authors.initials: C
authors.lastname: Zhu
mesh_terms: D001095:Arachidonic Acids / Q000494:pharmacology*; D001792:Blood Platelets / Q000378:metabolism*; D001793:Blood Preservation / Q000379:methods*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D063388:Endocannabinoids / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D010949:Plasma; D010983:Plateletpheresis; D053284:Polyunsaturated Alkamides / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D001095:Arachidonic Acids; D002121:Calcium Channel Blockers; D063388:Endocannabinoids; D053284:Polyunsaturated Alkamides; C078814:anandamide
keywords: Anandamide; Extended storage; Platelet storage lesions