title: Expression analysis of a stress-related phosphoinositide-specific phospholipase C gene in wheat (Triticum aestivum L.).
abstract: Plant phosphoinositide-specific phospholipases C (PI-PLCs) function in several essential plant processes associated with either development or environmental stress. In this report, we examined the expression patterns of TaPLC1 under drought and high salinity stress at the transcriptional and post-transcriptional levels. TaPLC1 mRNA was expressed in all wheat organs examined. U73122 and edelfosine, the PLC inhibitor, impaired seedling growth and enhanced seedling sensitivity to drought and high salinity stress. Though TaPLC1 expression in wheat was lowest at the seedling stage, it was strongly induced under conditions of stress. When 6-day-old wheat seedlings were treated with 200 mM NaCl or 20% (w/v) PEG 6000 for 6 or 12 h, respectively, the TaPLC1 transcript level increased by 16-fold compared to the control. Western blotting showed that the TaPLC protein concentration was also maintained at a high level from 24 to 48 h during stress treatment. Together, our results indicate the possible biological functions of TaPLC1 in regulating seedling growth and the response to drought and salinity stress.
authors.affiliation: College of Life Science, Agricultural University of Hebei, Baoding, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhang
authors.affiliation: College of Life Science, Agricultural University of Hebei, Baoding, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Jin
authors.affiliation: College of Life Science, Agricultural University of Hebei, Baoding, China.
authors.forename: Lizhu
authors.initials: L
authors.lastname: Wu
authors.affiliation: College of Life Science, Agricultural University of Hebei, Baoding, China.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Hou
authors.affiliation: College of Life Science, Agricultural University of Hebei, Baoding, China.
authors.forename: Shijuan
authors.initials: S
authors.lastname: Dou
authors.affiliation: College of Life Science, Agricultural University of Hebei, Baoding, China.
authors.forename: Yanyun
authors.initials: Y
authors.lastname: Pan
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D054801:Phosphoinositide Phospholipase C / Q000037:antagonists & inhibitors / Q000235:genetics*; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013312:Stress, Physiological; D014908:Triticum / Q000201:enzymology / Q000235:genetics*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D054801:Phosphoinositide Phospholipase C