title: Microtubule array reorientation in response to hormones does not involve changes in microtubule nucleation modes at the periclinal cell surface.
abstract: Aligned microtubule arrays spatially organize cell division, trafficking, and determine the direction of cell expansion in plant cells. In response to changes in environmental and developmental signals, cells reorganize their microtubule arrays into new configurations. Here, we tested the role of microtubule nucleation during hormone-induced microtubule array reorientation. We have found that in the process of microtubule array reorientation the ratios between branching, parallel, and de-novo nucleations remained constant, suggesting that the microtubule reorientation mechanism does not involve changes in nucleation modes. In the ton2/fass mutant, which has reduced microtubule branching nucleation frequency and decreased nucleation activity of the γ-tubulin complexes, microtubule arrays were able to reorient. Presented data suggest that reorientation of microtubules into transverse arrays in response to hormones does not involve changes in microtubule nucleation at the periclinal cell surface.
authors.affiliation: School of Biological Sciences, Illinois State University, Normal, IL 61790, USA.
authors.forename: Samantha
authors.initials: S
authors.lastname: Atkinson
authors.affiliation: School of Biological Sciences, Illinois State University, Normal, IL 61790, USA.
authors.forename: Angela
authors.initials: A
authors.lastname: Kirik
authors.affiliation: School of Biological Sciences, Illinois State University, Normal, IL 61790, USA vmkirik@ilstu.edu.
authors.forename: Viktor
authors.initials: V
authors.lastname: Kirik
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D017930:Genes, Reporter; D022101:Microtubule-Organizing Center / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure*; D009154:Mutation; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism*; D021381:Protein Transport; D011993:Recombinant Fusion Proteins; D014404:Tubulin / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C460109:FASS protein, Arabidopsis; D010937:Plant Growth Regulators; D011993:Recombinant Fusion Proteins; D014404:Tubulin; D010749:Phosphoprotein Phosphatases
keywords: microtubule cytoskeleton; microtubule nucleation.; microtubule organization; γ-tubulin complex