title: Quantum dots and p-phenylenediamine based method for the sensitive determination of glucose.
abstract: By introducing p-phenylenediamine (PPD) to the hybrid system of Mn-doped CdS/ZnS quantum dots (QDs) and glucose oxidase (GOD), a sensitive label-free method was proposed for direct detection of glucose. With glucose and PPD as substrates, 2,5-diamino-N,N'-di-(4-aminophenyl)-2,5-cyclohexadiene-1,4-diimine (DDACD) that intensively quenches the fluorescence of QDs can be produced by the catalysis of GOD. A detection limit as low as 3.2 μM was obtained with the high-efficient fluorescence quencher. Two linear ranges, from 5.0 μM to 1000 μM and from 1.0 mM to 10.0 mM, were identified between time-gated fluorescence intensity and the concentration of glucose. It is shown that the newly proposed methods have high selectivity for glucose over other saccharides and coexisting biological species in serum. The method can be used directly to determine glucose in normal adult human serum without any complicated sample pretreatments. The recovery rate and repeatability of the method were also shown to be satisfactory.
authors.affiliation: College of Environmental Science and Engineering, Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha 410082, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Lv
authors.affiliation: College of Environmental Science and Engineering, Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha 410082, China; College of Chemistry and Chemical Engineering, Xinxiang University, Xinxiang 453003, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Environmental Science and Engineering, Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha 410082, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Huang
authors.affiliation: College of Environmental Science and Engineering, Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha 410082, China. Electronic address: cgniu@hnu.edu.cn.
authors.forename: Chenggang
authors.initials: C
authors.lastname: Niu
authors.affiliation: College of Environmental Science and Engineering, Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha 410082, China.
authors.forename: Guangming
authors.initials: G
authors.lastname: Zeng
authors.affiliation: College of Environmental Science and Engineering, Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha 410082, China.
authors.forename: Qiuya
authors.initials: Q
authors.lastname: Niu
mesh_terms: D001786:Blood Glucose / Q000032:analysis*; D019187:Cadmium Compounds / Q000737:chemistry; D002384:Catalysis; D002623:Chemistry Techniques, Analytical; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry / Q000379:methods; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007097:Imines / Q000737:chemistry; D008345:Manganese / Q000737:chemistry; D010655:Phenylenediamines / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D045663:Quantum Dots; D012545:Schiff Bases / Q000737:chemistry; D012995:Solubility; D013440:Sulfides / Q000737:chemistry; D014867:Water / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: C000598589:2,5-diamino-N,N'-di-(4-aminophenyl)-2,5-cyclohexadiene-1,4-diimine; D001786:Blood Glucose; D019187:Cadmium Compounds; D007097:Imines; D010655:Phenylenediamines; D011134:Polysaccharides; D012545:Schiff Bases; D013440:Sulfides; D017967:Zinc Compounds; C034939:cadmium sulfide; D014867:Water; D008345:Manganese; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose; C031238:zinc sulfide; C029728:4-phenylenediamine
keywords: Glucose; Glucose oxidase; Quantum dots; Time-gated fluorescence; p-phenylenediamine