title: Fatty Acid Binding Protein 3 Is Involved in n-3 and n-6 PUFA transport in mouse trophoblasts.
abstract: BACKGROUND
  Low placental fatty acid (FA) transport during the embryonic period has been suggested to result in fetal developmental disorders and various adult metabolic diseases, but the molecular mechanism by which FAs are transported through the placental unit remains largely unknown.
  
  
  OBJECTIVE
  The aim of this study was to examine the distribution and functional relevance of FA binding protein (FABP), a cellular chaperone of FAs, in the mouse placenta.
  
  
  METHODS
  We clarified the localization of FABPs and sought to examine their function in placental FA transport through the phenotypic analysis of Fabp3-knockout mice.
  
  
  RESULTS
  Four FABPs (FABP3, FABP4, FABP5, and FABP7) were expressed with spatial heterogeneity in the placenta, and FABP3 was dominantly localized to the trophoblast cells. In placentas from the Fabp3-knockout mice (both sexes), the transport coefficients for linoleic acid (LA) were significantly reduced compared with those from wild-type mice by 25% and 44% at embryonic day (E) 15.5 and E18.5, respectively, whereas those for α-linolenic acid (ALA) were reduced by 19% and 17%, respectively. The accumulation of LA (18% and 27% at E15.5 and E18.5) and ALA (16% at E15.5) was also significantly less in the Fabp3-knockout fetuses than in wild-type fetuses. In contrast, transport and accumulation of palmitic acid (PA) were unaffected and glucose uptake significantly increased by 23% in the gene-ablated mice compared with wild-type mice at E18.5. Incorporation of LA (51% and 52% at 1 and 60 min, respectively) and ALA (23% at 60 min), but not PA, was significantly less in FABP3-knockdown BeWo cells than in controls, whereas glucose uptake was significantly upregulated by 51%, 50%, 31%, and 33% at 1, 20, 40, and 60 min, respectively.
  
  
  CONCLUSIONS
  Collectively FABP3 regulates n-3 (ω-3) and n-6 (ω-6) polyunsaturated FA transport in trophoblasts and plays a pivotal role in fetal development.
authors.affiliation: Department of Organ Anatomy.
authors.forename: Ariful
authors.initials: A
authors.lastname: Islam
authors.affiliation: Department of Organ Anatomy.
authors.forename: Yoshiteru
authors.initials: Y
authors.lastname: Kagawa
authors.affiliation: Department of Organ Anatomy.
authors.forename: Kazem
authors.initials: K
authors.lastname: Sharifi
authors.affiliation: Department of Organ Anatomy.
authors.forename: Majid
authors.initials: M
authors.lastname: Ebrahimi
authors.affiliation: Department of Organ Anatomy.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Miyazaki
authors.affiliation: Department of Organ Anatomy.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yasumoto
authors.affiliation: Department of Organ Anatomy.
authors.forename: Saki
authors.initials: S
authors.lastname: Kawamura
authors.affiliation: Department of Organ Anatomy.
authors.forename: Yui
authors.initials: Y
authors.lastname: Yamamoto
authors.affiliation: Institute of Radioisotope Research and Education, Science Research Center, Organization for Research Initiative, Yamaguchi University, Yamaguchi, Japan; and.
authors.forename: Syuiti
authors.initials: S
authors.lastname: Sakaguti
authors.affiliation: Department of Organ Anatomy.
authors.forename: Tomoo
authors.initials: T
authors.lastname: Sawada
authors.affiliation: Department of Health Science.
authors.forename: Nobuko
authors.initials: N
authors.lastname: Tokuda
authors.affiliation: Department of Obstetrics and Gynecology, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan.
authors.forename: Norihiro
authors.initials: N
authors.lastname: Sugino
authors.affiliation: Department of Anatomy, Akita University Graduate School of Medicine, Akita, Japan.
authors.forename: Ryoji
authors.initials: R
authors.lastname: Suzuki
authors.affiliation: Department of Organ Anatomy yowada@yamaguchi-u.ac.jp.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Owada
mesh_terms: D000818:Animals; D001692:Biological Transport; D000074026:Fatty Acid Binding Protein 3; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015525:Fatty Acids, Omega-3 / Q000378:metabolism*; D043371:Fatty Acids, Omega-6 / Q000378:metabolism*; D005260:Female; D005333:Fetus / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D014327:Trophoblasts / Q000187:drug effects / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: C493409:Fabp3 protein, mouse; C493411:Fabp4 protein, mouse; C082667:Fabp5 protein, mouse; C086936:Fabp7 protein, mouse; D000074026:Fatty Acid Binding Protein 3; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins