title: Serum levels of M-CSF, RANKL and OPG in rats fed with Kashin-Beck disease-affected diet.
abstract: OBJECTIVE
  There were no studies on the macrophage colony-stimulating factor (M-CSF), receptor activator of NF-kappaB ligand (RANKL) and osteoprotegerin (OPG) in the pathogenesis of Kashin-Beck disease (KBD). The objective of the present study was to investigate the serum M-CSF, RANKL and OPG in rats fed with KBD-affected diet.
  
  
  METHODS
  Ninety Wistar rats were divided into five groups. The rats received standard commercial feed with or without T-2 toxin additive, low protein feed with or without or T-2 toxin additive and the KBD-affected feed. The serum bioactivity of M-CSF, RANKL and OPG was tested by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The serum levels of M-CSF in E group rats were higher than those in the other groups in the five groups (P < 0.01). The serum levels of RANKL and OPG in E group rats were highest in the five groups and have significant difference compared to the other groups (P < 0.05).
  
  
  CONCLUSIONS
  The molecule of M-CSF, RANKL and OPG may be involved in the regulation of epiphyseal plate injury and repair in KBD, and its participation in the pathogenesis of KBD should be studied in the future.
authors.affiliation: Nanshan Hospital of Shenzhen University, Shenzhen 518052, People's Republic of China. ydlywl@126.com.
authors.forename: Denglu
authors.initials: D
authors.lastname: Yan
authors.forename: Fuxing
authors.initials: F
authors.lastname: Pei
authors.forename: Yancheng
authors.initials: Y
authors.lastname: Song
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D057767:Kashin-Beck Disease / Q000097:blood* / Q000139:chemically induced / Q000209:etiology; D016173:Macrophage Colony-Stimulating Factor / Q000097:blood*; D008297:Male; D053244:Osteoprotegerin / Q000097:blood; D053245:RANK Ligand / Q000097:blood*; D017208:Rats, Wistar; D013605:T-2 Toxin / Q000008:administration & dosage
chemical_list: D053244:Osteoprotegerin; D053245:RANK Ligand; D016173:Macrophage Colony-Stimulating Factor; D013605:T-2 Toxin