title: RNA interference of leptin receptor in chicken adipocytes.
abstract: In this study, chicken adipocytes were cultured to evaluate RNA interference by the leptin receptor gene. A small interfering RNA of the leptin receptor gene was synthesized, with a suppression rate of 60% being generated (P < 0.01). After the knockdown of the leptin receptor, the expression levels of certain genes decreased significantly; specifically, peroxisome proliferator-activated receptor γ, fatty acid synthase, adipose triglyceride lipase, and lipoprotein lipase. In addition, a significant increase in the expression of the adiponectin gene was documented. These results demonstrate that the leptin receptor gene might contribute to lipid metabolism by influencing the expressions of the peroxisome proliferator-activated receptor γ, fatty acid synthase, adipose triglyceride lipase, lipoprotein lipase, and adiponectin genes.
authors.affiliation: College of Animal Science, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: A X
authors.initials: AX
authors.lastname: Huang
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Li
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
authors.forename: J D
authors.initials: JD
authors.lastname: Shen
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
authors.forename: Z R
authors.initials: ZR
authors.lastname: Tao
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Li
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China lulizhibox@163.com.
authors.forename: L Z
authors.initials: LZ
authors.lastname: Lu
authors.affiliation: College of Animal Science, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Fu
authors.affiliation: College of Animal Science, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: T X
authors.initials: TX
authors.lastname: Wu
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D005786:Gene Expression Regulation; D050356:Lipid Metabolism / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000138:chemical synthesis / Q000235:genetics; D054411:Receptors, Leptin / Q000235:genetics*; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; D054411:Receptors, Leptin