title: Dextran synthesized by Leuconostoc mesenteroides BD1710 in tomato juice supplemented with sucrose.
abstract: The characteristics of the growth of Leuconostoc mesenteroides BD1710 and the synthesis of dextran in tomato juice supplemented with 15% sucrose were assayed. L. mesenteroides BD1710 could synthesize approximately 32 g L(-1) dextran in the tomato-juice-sucrose medium when cultured at 28 °C for 48 h, which was on the same level as the dextran yield in a chemically defined medium. The viscosity of the cultured tomato-juice-sucrose medium with various dextran contents was also measured. The results of the monosaccharide composition, molecular-weight distribution, Fourier transform infrared spectra (FTIR) and nuclear magnetic resonance spectra (NMR) showed that the polysaccharide synthesized by L. mesenteroides BD1710 in the tomato-juice-sucrose medium was dextran with a peak molecular weight of 6.35 × 10(5)Da, a linear backbone composed of consecutive α-(1 → 6)-linked d-glucopyranosyl units and approximately 6% α-(1 → 3) branches.
authors.affiliation: State Key Laboratory of Dairy Biotechnology, Shanghai 200436, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Han
authors.affiliation: Shanghai Engineering Research Center of Dairy Biotechnology, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Hang
authors.affiliation: State Key Laboratory of Dairy Biotechnology, Shanghai 200436, China.
authors.forename: Benheng
authors.initials: B
authors.lastname: Guo
authors.affiliation: Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai 200436, China.
authors.forename: Zhenmin
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Dairy Biotechnology, Shanghai 200436, China.
authors.forename: Chunpin
authors.initials: C
authors.lastname: You
authors.affiliation: State Key Laboratory of Dairy Biotechnology, Shanghai 200436, China. Electronic address: wuzhengjun@brightdairy.com.
authors.forename: Zhengjun
authors.initials: Z
authors.lastname: Wu
mesh_terms: D001628:Beverages; D003470:Culture Media / Q000737:chemistry; D003911:Dextrans / Q000096:biosynthesis* / Q000737:chemistry*; D005285:Fermentation; D005632:Fructose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007934:Leuconostoc / Q000254:growth & development / Q000378:metabolism*; D018551:Solanum lycopersicum; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared; D013395:Sucrose / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
chemical_list: D003470:Culture Media; D003911:Dextrans; D009005:Monosaccharides; D005632:Fructose; D013395:Sucrose; D005947:Glucose
keywords: Dextran; Leuconostoc mesenteroides BD1710; Tomato juice sucrose medium