title: Nitinol-based nanotubular coatings for the modulation of human vascular cell function.
abstract: In this study, we describe the synthesis of an upright nanotubular coating with discrete, exposed nanotubes on top of superelastic Nitinol via anodization and characterization of the surface elemental composition and nickel release rates. We demonstrate, for the first time, that this coating could improve re-endothelialization by increasing the cell spreading and migration of primary human aortic endothelial cells on Nitinol. We also show the potential for reducing neointimal hyperplasia by decreasing the proliferation and expression of collagen I and MMP-2 in primary human aortic smooth muscle cells (HASMC). Furthermore, we did not observe the nanotubular surface to induce inflammation through ICAM-1 expression in HASMC as compared to the flat control. This coating could be used to improve Nitinol stents by reducing restenosis rates and, given the extensive use of Nitinol in other implantable devices, act as a generalized coating strategy for other medical devices.
authors.affiliation: Department of Bioengineering and Therapeutic Sciences, University of California-San Francisco , San Francisco, California 94158, United States.
authors.forename: Phin P
authors.initials: PP
authors.lastname: Lee
authors.forename: Alec
authors.initials: A
authors.lastname: Cerchiari
authors.forename: Tejal A
authors.initials: TA
authors.lastname: Desai
mesh_terms: D000497:Alloys / Q000737:chemistry*; D001011:Aorta / Q000166:cytology; D001672:Biocompatible Materials / Q000737:chemistry; D002465:Cell Movement; D049109:Cell Proliferation; D024042:Collagen Type I / Q000378:metabolism; D023903:Coronary Restenosis / Q000473:pathology; D042783:Endothelial Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D007249:Inflammation; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D036103:Nanotechnology / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry; D015607:Stents; D013499:Surface Properties
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D024042:Collagen Type I; D018799:Intercellular Adhesion Molecule-1; C013616:nitinol; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2
keywords: Human Aortic Endothelial Cells; Human Aortic Smooth Muscle Cells; Nanotubes; Nitinol; Restenosis; Stents