title: Effect of electroporation on bioconversion of isoflavones and probiotic properties of parents and subsequent passages of Bifidobacterium longum.
abstract: This study aimed to evaluate the effects of electroporation on growth, bioconversion of isoflavones, and probiotic properties of parent organisms and subsequent passages of Bifidobacterium longum FTDC 8643. Electroporation with the strength of electric field at 7.5 kV cm(-1) for 3.5 ms was applied on B. longum FTDC 8643. The viability of B. longum FTDC 8643 increased significantly upon treatment with electroporation. Such treatment also enhanced the intracellular and extracellular β-glucosidase activity, leading to enhanced production of bioactive isoflavone aglycones in mannitol-soymilk (P < 0.05). In addition, these treated cells also exhibited better tolerance toward acidic (pH 2 and pH 3) and intestinal bile salt condition compared to the control (P < 0.05). The electroporated cell also possessed better adhesion ability and antimicrobial activity (P < 0.05). However, all these positive effects were only prevalent in the parent cells and were not observed in their subsequent passages of electroporated cells. Our results suggested that electroporation could enhance the bioactive and probiotic potentials of parent cells of B. longum FTDC 8643 and could be used in the production of probiotic foods with enhanced bioactivity.
authors.affiliation: Bioprocess Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800, Penang, Malaysia.
authors.forename: Siok-Koon
authors.initials: SK
authors.lastname: Yeo
authors.affiliation: Bioprocess Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800, Penang, Malaysia.
authors.forename: Jia-Sin
authors.initials: JS
authors.lastname: Ong
authors.affiliation: Bioprocess Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800, Penang, Malaysia. mintze.liong@usm.my.
authors.forename: Min-Tze
authors.initials: MT
authors.lastname: Liong
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects; D001644:Bifidobacterium / Q000254:growth & development*; D018274:Electroporation; D006863:Hydrogen-Ion Concentration; D007529:Isoflavones / Q000096:biosynthesis*; D008353:Mannitol / Q000494:pharmacology; D050296:Microbial Viability / Q000187:drug effects; D019936:Probiotics
chemical_list: D007529:Isoflavones; D008353:Mannitol