title: Lubricating recovery of damaged pleural mesothelium: effect of time and of phosphatidylcholines.
abstract: Effect of time and phosphatidylcholines (PCs) on lubrication of damaged mesothelium has been investigated. Marked increase in coefficient of kinetic friction (μ) of pleural specimens after mesothelial blotting and rewetting decreased by 23.4±3.5%, 41.8±3.8%, and 40.5±2.7% after 30min, 1h, and 2h. Hence, damaged mesothelium is able to partially reset lubricating molecules on its surface. Increase in μ of post-blotting Ringer 2h after addition of unsaturated PCs (3mg/ml) decreased a little more than after 2h Ringer. Effects of unsaturated and saturated PCs were similar, contrary to expectation raised by their different percentage in pleural and alveolar lavage. Effect of PCs did not increase at 6mg/ml, and was nil at 0.4mg/ml. Increase of μ after short phospholipase treatment decreased by 45.9±2.0% after 2h Ringer, and a little more after addition of unsaturated or saturated PCs. Hence, PCs, as other phospholipids, have a small effect, likely because of difficulty in resetting their relationships with main lubricating molecules.
authors.affiliation: Dipartimento di Fisiopatologia e dei Trapianti, Sezione di Fisiologia Umana, Università degli studi di Milano, Milan, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Bodega
authors.affiliation: Dipartimento di Fisiopatologia e dei Trapianti, Sezione di Fisiologia Umana, Università degli studi di Milano, Milan, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Sironi
authors.affiliation: Dipartimento di Fisiopatologia e dei Trapianti, Sezione di Fisiologia Umana, Università degli studi di Milano, Milan, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Porta
authors.affiliation: Dipartimento di Fisiopatologia e dei Trapianti, Sezione di Fisiologia Umana, Università degli studi di Milano, Milan, Italy. Electronic address: emilio.agostoni@unimi.it.
authors.forename: Emilio
authors.initials: E
authors.lastname: Agostoni
mesh_terms: D000818:Animals; D003964:Diaphragm / Q000166:cytology / Q000502:physiology*; D004848:Epithelium / Q000502:physiology; D008153:Lubrication; D008168:Lung / Q000166:cytology / Q000502:physiology*; D010713:Phosphatidylcholines / Q000008:administration & dosage* / Q000494:pharmacology; D010994:Pleura / Q000187:drug effects / Q000293:injuries*; D011817:Rabbits; D013997:Time Factors
chemical_list: D010713:Phosphatidylcholines
keywords: Friction coefficient; Mesothelial glycocalyx; Mesothelium recovery; Mucins; Phosphatidylcholines; Phospholipase