title: Localization of MLH3 at the centrosomes.
abstract: Mutations in human DNA mismatch repair (MMR) genes are commonly associated with hereditary nonpolyposis colorectal cancer (HNPCC). MLH1 protein heterodimerizes with PMS2, PMS1, and MLH3 to form MutLα, MutLβ, and MutLγ, respectively. We reported recently stable expression of GFP-linked MLH3 in human cell lines. Monitoring these cell lines during the cell cycle using live cell imaging combined with confocal microscopy, we detected accumulation of MLH3 at the centrosomes. Fluorescence recovery after photobleaching (FRAP) revealed high mobility and fast exchange rates at the centrosomes as it has been reported for other DNA repair proteins. MLH3 may have a role in combination with other repair proteins in the control of centrosome numbers.
authors.affiliation: Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Department of Human and Animal Cell Lines, Braunschweig 38124, Germany. roesner.lennart@mh-hannover.de.
authors.forename: Lennart M
authors.initials: LM
authors.lastname: Roesner
authors.affiliation: Institute of Clinical Chemistry and Laboratory Diagnostics, Heinrich-Heine-University, Medical School, Düsseldorf 40225, Germany. christian.mielke@med.uni-duesseldorf.de.
authors.forename: Christian
authors.initials: C
authors.lastname: Mielke
authors.affiliation: Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Department of Human and Animal Cell Lines, Braunschweig 38124, Germany. sif@dsmz.de.
authors.forename: Silke
authors.initials: S
authors.lastname: Faehnrich
authors.affiliation: Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Department of Human and Animal Cell Lines, Braunschweig 38124, Germany. yvonne.merkhoffer@dsmz.de.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Merkhoffer
authors.affiliation: Helmholtz Centre of Infection Research, Braunschweig 38124, Germany. ked@helmholtz-hzi.de.
authors.forename: Kurt E J
authors.initials: KE
authors.lastname: Dittmar
authors.affiliation: Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Department of Human and Animal Cell Lines, Braunschweig 38124, Germany. hdr@dsmz.de.
authors.forename: Hans G
authors.initials: HG
authors.lastname: Drexler
authors.affiliation: Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Department of Human and Animal Cell Lines, Braunschweig 38124, Germany. wdi@dsmz.de.
authors.forename: Wilhelm G
authors.initials: WG
authors.lastname: Dirks
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D018385:Centrosome / Q000378:metabolism*; D036681:Fluorescence Recovery After Photobleaching; D057809:HEK293 Cells; D006801:Humans; D018613:Microscopy, Confocal; D000070956:MutL Proteins
chemical_list: D002352:Carrier Proteins; C403623:MLH3 protein, human; D000070956:MutL Proteins