title: Modification of carboxymethyl cellulose grafted with collagen peptide and its antioxidant activity.
abstract: Carboxymethyl cellulose used in wounds has little antioxidant capacity. The aim of the present study was to improve the scavenging ability of carboxymethyl cellulose by modified with collagen peptide. The reaction conditions have been optimized by varying mass ratio of collagen peptide to carboxymethyl cellulose, temperature and reaction time. Antioxidant activities of carboxymethyl cellulose derivatives (CMCC) were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, superoxide radicals and the reducing power. The effects of concentration, degree of substitution (DS) and molecular weight on three different radicals scavenging activity and reducing power were examined. Methylthiazol tetrazolium (MTT) assay was used to evaluate the fibroblasts cells cytotoxicity of CMCC. Results showed that the scavenging effects of CMCC increased with the increasing of DS and concentration. This product of CMCC possesses a distinct antioxidant capacity on radicals.
authors.affiliation: College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China. Electronic address: lihongfan2000@hotmail.com.
authors.forename: Lihong
authors.initials: L
authors.lastname: Fan
authors.affiliation: College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Min
authors.initials: M
authors.lastname: Peng
authors.affiliation: College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhou
authors.affiliation: College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Wu
authors.affiliation: College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Hu
authors.affiliation: The Third Hospital of Wuhan, Wuhan 430060, China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Xie
authors.affiliation: The Third Hospital of Wuhan, Wuhan 430060, China.
authors.forename: Shuhua
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry* / Q000494:pharmacology*; D002460:Cell Line / Q000187:drug effects; D060326:Chemistry Techniques, Synthetic; D003094:Collagen / Q000737:chemistry*; D005347:Fibroblasts / Q000187:drug effects; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D008970:Molecular Weight; D010851:Picrates / Q000737:chemistry; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D013329:Structure-Activity Relationship; D013696:Temperature
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D003094:Collagen; C004931:1,1-diphenyl-2-picrylhydrazyl; D002266:Carboxymethylcellulose Sodium
keywords: Antioxidant activity; Carboxymethyl cellulose; Collagen peptide; Fibroblasts cells; Free radical