title: Islet oxygen consumption rate dose predicts insulin independence for first clinical islet allotransplants.
abstract: BACKGROUND
  Human islet allotransplantation for the treatment of type 1 diabetes is in phase III clinical trials in the U.S. and is the standard of care in several other countries. Current islet product release criteria include viability based on cell membrane integrity stains, glucose-stimulated insulin release, and islet equivalent (IE) dose based on counts. However, only a fraction of patients transplanted with islets that meet or exceed these release criteria become insulin independent following 1 transplant. Measurements of islet oxygen consumption rate (OCR) have been reported as highly predictive of transplant outcome in many models.
  
  
  METHODS
  In this article we report on the assessment of clinical islet allograft preparations using OCR dose (or viable IE dose) and current product release assays in a series of 13 first transplant recipients. The predictive capability of each assay was examined and successful graft function was defined as 100% insulin independence within 45 days post-transplant.
  
  
  RESULTS
  OCR dose was most predictive of CTO. IE dose was also highly predictive, while glucoses stimulated insulin release and membrane integrity stains were not.
  
  
  CONCLUSIONS
  OCR dose can predict CTO with high specificity and sensitivity and is a useful tool for evaluating islet preparations prior to clinical human islet allotransplantation.
authors.affiliation: Department of Surgery, University of Arizona, Tucson, AZ.
authors.forename: J P
authors.initials: JP
authors.lastname: Kitzmann
authors.affiliation: Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: D
authors.initials: D
authors.lastname: O'Gorman
authors.affiliation: Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: T
authors.initials: T
authors.lastname: Kin
authors.affiliation: Department of Surgery, University of Arizona, Tucson, AZ.
authors.forename: A C
authors.initials: AC
authors.lastname: Gruessner
authors.affiliation: Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Senior
authors.affiliation: Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Imes
authors.affiliation: Department of Surgery, University of Arizona, Tucson, AZ.
authors.forename: R W
authors.initials: RW
authors.lastname: Gruessner
authors.affiliation: Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: A M J
authors.initials: AM
authors.lastname: Shapiro
authors.affiliation: Department of Surgery, University of Arizona, Tucson, AZ. Electronic address: kkpapas@surgery.arizona.edu.
authors.forename: K K
authors.initials: KK
authors.lastname: Papas
mesh_terms: D015331:Cohort Studies; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism* / Q000601:surgery*; D006801:Humans; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism*; D016381:Islets of Langerhans Transplantation; D010101:Oxygen Consumption / Q000502:physiology*; D011237:Predictive Value of Tests; D012372:ROC Curve; D014184:Transplantation, Homologous; D016896:Treatment Outcome
chemical_list: D007328:Insulin