title: MLPA Application in Clinical Diagnosis of DMD/BMD in Shanghai.
abstract: BACKGROUND
  Duchenne and Becker muscular dystrophy (DMD/BMD) are X-linked recessive disorders caused by mutation in dystrophin gene. We reported 3-year clinic experience from a single hospital in Shanghai using multiplex ligation dependent probe amplification (MLPA) assay to detect DMD mutations.
  
  
  METHODS
  Four hundred and fifty-one males and 184 females, who were clinically diagnosed as DMD/BMD patients or carriers at our hospital's outpatient clinic, were collected and performed with MLPA to detect DMD gene mutations.
  
  
  RESULTS
  Seventeen novel mutation points not reported in the Leiden Muscular Dystrophy pages were identified in this study. We found that the most frequent deletion spots ranged from exon45 to exon52, and exon2, exon19 were the two most frequently detected duplication spots.
  
  
  CONCLUSIONS
  The results of our study confirmed MLPA as an efficient clinical method for detecting DMD gene mutations in DMD/BMD patients. Single exon mutation detected by MLPA should be verified by other methods, and we should emphasize that only precise clinical molecular diagnosis can lead to the feasibility of prenatal diagnosis.
authors.affiliation: Department of Prenatal Diagnosis Center, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.|Shanghai Institute for Pediatric Research, Shanghai, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Ji
authors.affiliation: Department of Prenatal Diagnosis Center, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.|Shanghai Institute for Pediatric Research, Shanghai, China.
authors.forename: Jingmin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Prenatal Diagnosis Center, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.|Shanghai Institute for Pediatric Research, Shanghai, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Shanghai Institute for Pediatric Research, Shanghai, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Long
authors.affiliation: Shanghai Institute for Pediatric Research, Shanghai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: Shanghai Institute for Pediatric Research, Shanghai, China.|Department of Neurology, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Prenatal Diagnosis Center, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.|Shanghai Institute for Pediatric Research, Shanghai, China.
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Prenatal Diagnosis Center, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.|Shanghai Institute for Pediatric Research, Shanghai, China.
authors.forename: Wenting
authors.initials: W
authors.lastname: Jiang
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D002681:China; D016189:Dystrophin / Q000235:genetics*; D005091:Exons / Q000235:genetics; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D020388:Muscular Dystrophy, Duchenne / Q000175:diagnosis* / Q000235:genetics; D009154:Mutation / Q000235:genetics; D055815:Young Adult
chemical_list: C484258:DMD protein, human; D016189:Dystrophin
keywords: BMD; DMD; MLPA; clinical diagnosis; mutation