title: Effect of MLH1 -93G>A on gene expression in patients with colorectal cancer.
abstract: The DNA repair machinery plays a key role in maintaining genomic stability by preventing the emergence of mutations. Furthermore, the -93G>A polymorphism in the MLH1 gene has been associated with an increased risk of developing colorectal cancer. Therefore, the aim of this study was to examine the expression pattern and effect of this polymorphism in normal and tumour samples from patients with colorectal cancer. The MLH1 -93G>A (rs1800734) polymorphism was detected by PCR-RFLP in 49 cases of colorectal cancer. MLH1 expression was investigated using real-time quantitative PCR. The results indicate a significant decrease in MLH1 expression in tumour samples compared to their normal counterparts. The MLH1 gene was also significantly repressed in samples from patients who had some degree of tumour invasion into other organs. Similarly, those patients who were in a more advanced tumour stage (TNM III and IV) exhibited a significant reduction in MLH1 gene expression. Finally, the mutant genotype AA of MLH1 was associated with a significant decrease in the expression of this gene. This finding suggests that this polymorphism could increase the risk of developing colorectal cancer by a defective mismatch repair system, particularly through the loss of MLH1 expression in an allele-specific manner.
authors.affiliation: Unidade Integrada de Farmacologia e Gastroenterologia, Laboratory of Microbiology and Molecular Biology, Sao Francisco University Medical School, Av. São Francisco de Assis, 218. Jd. São José, Bragança Paulista, SP, Brazil.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Funck
authors.forename: Juliana C
authors.initials: JC
authors.lastname: Santos
authors.forename: Isabelle J L
authors.initials: IJ
authors.lastname: Silva-Fernandes
authors.forename: Silvia H B
authors.initials: SH
authors.lastname: Rabenhorst
authors.forename: Carlos A R
authors.initials: CA
authors.lastname: Martinez
authors.forename: Marcelo L
authors.initials: ML
authors.lastname: Ribeiro
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015331:Cohort Studies; D015179:Colorectal Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism; D004260:DNA Repair; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D000070957:MutL Protein Homolog 1; D009687:Nuclear Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C501486:MLH1 protein, human; D009687:Nuclear Proteins; D000070957:MutL Protein Homolog 1