title: Branched-chain amino acid supplemented diet during maternal food restriction prevents developmental hypertension in adult rat offspring.
abstract: Maternal food restriction is known to cause developmental hypertension in offspring. We have previously shown that maternal high-protein diet can reverse fetal programming of hypertension and that branched-chain amino acid (BCAA) concentrations in maternal and fetal plasma were increased by maternal high-protein intake. Then, we hypothesized that isocaloric supplementation with BCAA to a maternal food restriction can reverse the adverse outcome. Pregnant rats were divided into four groups at 7.5 days postcoitum: normally nourished (NN) and 70% undernourished (UN) groups with and without BCAA supplementation (NN-standard diet (SD), NN-BCAA, UN-SD and UN-BCAA groups). Compared with pups in the NN groups, those in the UN-SD group had significantly increased systolic blood pressure (SBP) at 8 and 16 weeks of age (P < 0.05). However, the elevation of SBP was not observed in offspring in the UN-BCAA group. Offspring glomeruli number of the UN groups was significantly lower (P < 0.05) than that of the NN groups, independent of BCAA supplementation. Angiotensin II receptor type 2 (ATR2) mRNA and protein expression in the kidney was significantly augmented in the UN-BCAA group at 30 weeks of age. In conclusion, BCAA supplementation during maternal food restriction prevents developmental hypertension together with increased ATR2 expression in adult offspring kidney.
authors.affiliation: 1Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Fujii
authors.affiliation: 1Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yura
authors.affiliation: 1Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tatsumi
authors.affiliation: 1Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Kondoh
authors.affiliation: 1Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Mogami
authors.affiliation: 1Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Fujita
authors.affiliation: 1Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kakui
authors.affiliation: 3Department of Home Economics, Otsuma Women's University, 12, Sanban-cho, Chiyoda-ku, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Aoe
authors.affiliation: 4Department of Obstetrics and Gynecology, Hamamatsu University, School of Medicine, Hamamatsu, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Itoh
authors.affiliation: 5Department of Obstetrics and Gynecology, Mie University Graduate School of Medicine, Tsu, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Sagawa
authors.affiliation: 6Department of Gynecology and Obstetrics, National Hospital Organization, Kyoto Medical Center, Kyoto, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Fujii
authors.affiliation: 1Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Konishi