title: The synaptoneurosome transcriptome: a model for profiling the emolecular effects of alcohol.
abstract: Chronic alcohol consumption changes gene expression, likely causing persistent remodeling of synaptic structures via altered translation of mRNAs within synaptic compartments of the cell. We profiled the transcriptome from synaptoneurosomes (SNs) and paired total homogenates (THs) from mouse amygdala following chronic voluntary alcohol consumption. In SN, both the number of alcohol-responsive mRNAs and the magnitude of fold-change were greater than in THs, including many GABA-related mRNAs upregulated in SNs. Furthermore, SN gene co-expression analysis revealed a highly connected network, demonstrating coordinated patterns of gene expression and highlighting alcohol-responsive biological pathways, such as long-term potentiation, long-term depression, glutamate signaling, RNA processing and upregulation of alcohol-responsive genes within neuroimmune modules. Alterations in these pathways have also been observed in the amygdala of human alcoholics. SNs offer an ideal model for detecting intricate networks of coordinated synaptic gene expression and may provide a unique system for investigating therapeutic targets for the treatment of alcoholism.
authors.affiliation: 1] Waggoner Center for Alcohol and Addiction Research, University of Texas at Austin, Austin, TX, USA [2] The Institute for Neuroscience (INS), University of Texas at Austin, Austin, TX, USA.
authors.forename: D
authors.initials: D
authors.lastname: Most
authors.affiliation: 1] Waggoner Center for Alcohol and Addiction Research, University of Texas at Austin, Austin, TX, USA [2] The Institute for Neuroscience (INS), University of Texas at Austin, Austin, TX, USA.
authors.forename: L
authors.initials: L
authors.lastname: Ferguson
authors.affiliation: Waggoner Center for Alcohol and Addiction Research, University of Texas at Austin, Austin, TX, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Blednov
authors.affiliation: Waggoner Center for Alcohol and Addiction Research, University of Texas at Austin, Austin, TX, USA.
authors.forename: R D
authors.initials: RD
authors.lastname: Mayfield
authors.affiliation: Waggoner Center for Alcohol and Addiction Research, University of Texas at Austin, Austin, TX, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Harris
mesh_terms: D000428:Alcohol Drinking / Q000235:genetics*; D000437:Alcoholism / Q000235:genetics / Q000378:metabolism; D000679:Amygdala / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D000431:Ethanol / Q000009:adverse effects*; D005260:Female; D015870:Gene Expression / Q000187:drug effects* / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods; D053263:Gene Regulatory Networks / Q000187:drug effects* / Q000235:genetics*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D059467:Transcriptome / Q000187:drug effects / Q000235:genetics*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D000431:Ethanol; D005680:gamma-Aminobutyric Acid