title: Antioxidant and immunological activities of polysaccharides from Gentiana scabra Bunge roots.
abstract: Two polysaccharide fractions, GSP I-a and GSP II-b, were isolated from Gentiana scabra Bunge roots. Both GSP I-a and GSP II-b comprised seven monosaccharides: fructose, mannose, rhamnose, galacturonic acid, glucose, galactose, and fucose. Ultraviolet and infrared analyses show that GSP I-a and GSP II-b are proteoglycans. In vitro evaluation of the antioxidant activity suggests that GSP I-a and GSP II-b scavenge 1,1-diphenyl-2-picrylhydrazyl radicals. However, the scavenging activity of the latter is stronger than that of the former. GSP I-a and GSP II-b have relatively low reducing powers and scavenging activities toward superoxide anions and hydroxyls. GSP I-a and GSP II-b significantly increase lymphocyte proliferation when lipopolysaccharide is used as a mitogen for lymphocytes, but only GSP I-a can significantly increase lymphocyte proliferation within the test-dosage range when concanavalin A is used as a mitogen.
authors.affiliation: College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, PR China. Electronic address: wangzy125@gmail.com.
authors.forename: Zhanyong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, PR China.
authors.forename: Chenyu
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Su
authors.affiliation: College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D016166:Free Radical Scavengers / Q000494:pharmacology; D030012:Gentiana / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D008807:Mice, Inbred BALB C; D009005:Monosaccharides / Q000032:analysis; D018517:Plant Roots / Q000737:chemistry; D011134:Polysaccharides / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D009005:Monosaccharides; D011134:Polysaccharides; D013481:Superoxides; D017665:Hydroxyl Radical
keywords: Antioxidant; Gentiana scabra Bunge; Lymphocyte proliferation