title: Random mutagenesis and media optimisation for hyperproduction of cellulase from Bacillus species using proximally analysed Saccharum spontaneum in submerged fermentation.
abstract: This study deals with the isolation of novel mutant of Bacillus and optimisation of media for the hyperproduction of cellulase. Cellulase-producing Bacillus PC-BC6 was subjected to physical and chemical mutagenesis to enhance the cellulolytic potential. Later, mutagenesis isolates were screened both qualitatively and quantitatively. Among all the tested isolates, Bacillus N3 yielded maximum (CMCase 1250 IU/mL/min and FPase 629 IU/mL/min) activity. The Bacillus N3 strain exhibited 1.7-fold more enzyme production as compared with the parental strain. Proximate analysis of untreated and pretreated Saccharum spontaneum was carried out to improve cellulase production. Three different media were tested for the production of cellulase, among which M2 medium containing MgSO4, pretreated S. spontaneum, K2HPO4, (NH4)2SO4 and peptone was found to be the best for maximum enzyme production by mutant Bacillus N3.
authors.affiliation: a Department of Biotechnology, Lahore College for Women University , Lahore , Pakistan.
authors.forename: Roheena
authors.initials: R
authors.lastname: Abdullah
authors.forename: Wajeeha
authors.initials: W
authors.lastname: Zafar
authors.forename: Muhammad
authors.initials: M
authors.lastname: Nadeem
authors.forename: Mehwish
authors.initials: M
authors.lastname: Iqtedar
authors.forename: Shagufta
authors.initials: S
authors.lastname: Naz
authors.forename: Quratulain
authors.initials: Q
authors.lastname: Syed
authors.forename: Zahid Ali
authors.initials: ZA
authors.lastname: Butt
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D002480:Cellulase / Q000096:biosynthesis*; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D016296:Mutagenesis; D031786:Saccharum
chemical_list: D003470:Culture Media; D002480:Cellulase
keywords: Bacillus; cellulases; mutation; nitrous acid; submerged fermentation