title: Development of eight novel microsatellite markers for Huoyan geese.
abstract: In this study, we isolated microsatellite DNA from the Huoyan goose genome with magnetic beads. As a result, 150 positive clones were identified, and 148 microsatellites were found. Among the 148 microsatellites, 69.6% were perfect, 17.6% were imperfect, and the rest were compound type (12.8%). Twenty microsatellite primers were used to screen 90 individuals from 3 Huoyan goose populations. Eight loci were polymorphic with a low number of alleles (2 to 4). The observed and expected heterozygosities ranged from 0.3556 to 1 and from 0.2923 to 0.6868, respectively. All the 8 polymorphic loci were in Hardy-Weinberg equilibrium. These molecular markers will be useful for future studies on population genetic structure and conservation genetics in Huoyan geese.
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning, China.
authors.forename: Z Z
authors.initials: ZZ
authors.lastname: Cao
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning, China.
authors.forename: D
authors.initials: D
authors.lastname: Su
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning, China.
authors.forename: M
authors.initials: M
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning, China.
authors.forename: M
authors.initials: M
authors.lastname: Gao
authors.affiliation: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning, China xhluan@163.com.
authors.forename: X H
authors.initials: XH
authors.lastname: Luan
mesh_terms: D000483:Alleles; D000818:Animals; D005777:Geese / Q000235:genetics*; D056426:Genetic Loci; D014644:Genetic Variation; D005828:Genetics, Population; D005838:Genotype; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data