title: Characterization of Coffee ringspot virus-Lavras: a model for an emerging threat to coffee production and quality.
abstract: The emergence of viruses in Coffee (Coffea arabica and Coffea canephora), the most widely traded agricultural commodity in the world, is of critical concern. The RNA1 (6552nt) of Coffee ringspot virus is organized into five open reading frames (ORFs) capable of encoding the viral nucleocapsid (ORF1p), phosphoprotein (ORF2p), putative cell-to-cell movement protein (ORF3p), matrix protein (ORF4p) and glycoprotein (ORF5p). Each ORF is separated by a conserved intergenic junction. RNA2 (5945nt), which completes the bipartite genome, encodes a single protein (ORF6p) with homology to RNA-dependent RNA polymerases. Phylogenetic analysis of L protein sequences firmly establishes CoRSV as a member of the recently proposed Dichorhavirus genus. Predictive algorithms, in planta protein expression, and a yeast-based nuclear import assay were used to determine the nucleophillic character of five CoRSV proteins. Finally, the temperature-dependent ability of CoRSV to establish systemic infections in an initially local lesion host was quantified.
authors.affiliation: Universidade Federal de Lavras, Departamento de Fitopatologia, Caixa Postal 3037, CEP 37200-000 Lavras, MG, Brasil.
authors.forename: T O
authors.initials: TO
authors.lastname: Ramalho
authors.affiliation: Universidade Federal de Lavras, Departamento de Fitopatologia, Caixa Postal 3037, CEP 37200-000 Lavras, MG, Brasil.
authors.forename: A R
authors.initials: AR
authors.lastname: Figueira
authors.affiliation: Universidade Federal de Lavras, Departamento de Fitopatologia, Caixa Postal 3037, CEP 37200-000 Lavras, MG, Brasil.
authors.forename: A J
authors.initials: AJ
authors.lastname: Sotero
authors.affiliation: Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA.
authors.forename: R
authors.initials: R
authors.lastname: Wang
authors.affiliation: Universidade Federal de Lavras, Departamento de Fitopatologia, Caixa Postal 3037, CEP 37200-000 Lavras, MG, Brasil.
authors.forename: P S
authors.initials: PS
authors.lastname: Geraldino Duarte
authors.affiliation: Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA.
authors.forename: M
authors.initials: M
authors.lastname: Farman
authors.affiliation: Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA. Electronic address: mgoodin@uky.edu.
authors.forename: M M
authors.initials: MM
authors.lastname: Goodin
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D040503:Coffea / Q000737:chemistry / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012209:Rhabdoviridae / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry / Q000821:virology; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: Brazil; Climate change; Dichorhavirus; Negative-strand RNA virus; Protein localization; Rhabdovirus; Virus emergence