title: Development and validation of a sensitive and specific sodB-based quantitative PCR assay for molecular detection of Ehrlichia species.
abstract: We developed a sensitive and specific sodB-based quantitative PCR assay to detect Ehrlichia spp. The assay's limit of detection was 5 copies/reaction, and it did not amplify nonspecific DNA. Compared with a 16S rRNA gene PCR target, the sodB target may offer an improved molecular diagnostic assay to detect Ehrlichia spp.
authors.affiliation: Intracellular Pathogens Research Laboratory and the Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA.
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Qurollo
authors.affiliation: Intracellular Pathogens Research Laboratory and the Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA.
authors.forename: Dana
authors.initials: D
authors.lastname: Riggins
authors.affiliation: Intracellular Pathogens Research Laboratory and the Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA.
authors.forename: Alaire
authors.initials: A
authors.lastname: Comyn
authors.affiliation: Intracellular Pathogens Research Laboratory and the Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA.
authors.forename: Merone T
authors.initials: MT
authors.lastname: Zewde
authors.affiliation: Intracellular Pathogens Research Laboratory and the Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA ebbreits@ncsu.edou.
authors.forename: Edward B
authors.initials: EB
authors.lastname: Breitschwerdt
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004285:Dogs; D004536:Ehrlichia / Q000235:genetics* / Q000302:isolation & purification*; D016873:Ehrlichiosis / Q000175:diagnosis* / Q000382:microbiology / Q000662:veterinary; D006801:Humans; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013482:Superoxide Dismutase / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; C087504:SodB protein, Bacteria; D013482:Superoxide Dismutase