title: Activated-ester-type photocleavable crosslinker for preparation of photodegradable hydrogels using a two-component mixing reaction.
abstract: Photodegradable hydrogels have emerged as powerful platforms for studying and directing cellular behavior in a spatiotemporally controlled manner. Photodegradable hydrogels have previously been formed by free radical polymerizations, Michael-type addition reactions, and orthogonal click reactions. Here, an ester-activated photocleavable crosslinker is presented for preparing photodegradable hydrogels by means of a one-step mixing reaction between the crosslinker and a biocompatible polymer containing amino moieties (amino-terminated tetra-arm poly(ethylene glycol) or gelatin). It is demonstrated that photodegradable hydrogels micropatterned by photolithography can be used to culture cells with high viability and proliferation rates. The resulting micropatterned cell-laden structures can potentially be used to create 3D biomaterials for various tissue-engineering applications.
authors.affiliation: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.
authors.forename: Fumiki
authors.initials: F
authors.lastname: Yanagawa
authors.forename: Shinji
authors.initials: S
authors.lastname: Sugiura
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Takagi
authors.forename: Kimio
authors.initials: K
authors.lastname: Sumaru
authors.forename: Gulden
authors.initials: G
authors.lastname: Camci-Unal
authors.forename: Alpesh
authors.initials: A
authors.lastname: Patel
authors.forename: Ali
authors.initials: A
authors.lastname: Khademhosseini
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kanamori
mesh_terms: D002470:Cell Survival / Q000528:radiation effects; D003432:Cross-Linking Reagents / Q000737:chemistry*; D055119:Elastic Modulus / Q000528:radiation effects; D004952:Esters / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008027:Light; D010782:Photolysis / Q000528:radiation effects*; D013997:Time Factors
chemical_list: D003432:Cross-Linking Reagents; D004952:Esters; D020100:Hydrogels
keywords: crosslinkers; hydrogels; micropatterning; photodegradation