title: High cell density lipid rich cultivation of a novel microalgal isolate Chlorella sorokiniana FC6 IITG in a single-stage fed-batch mode under mixotrophic condition.
abstract: A single-stage mixotrophic cultivation strategy was developed with a novel microalgal isolate Chlorella sorokiniana FC6 IITG for high cell density lipid-rich biomass generation. The strain was evaluated for growth and lipid content under different physico-chemical parameters, nutritional conditions and trophic modes. Finally, a single-stage mixotrophic fed-batch cultivation strategy was demonstrated with intermittent feeding of key nutrients along with dynamic increase in light intensity for high cell density biomass and sodium acetate as elicitor for lipid enrichment. The key findings: (i) glucose and sodium acetate was identified as growth supporting and lipid inducing nutrients, respectively; (ii) mixotrophic batch cultivation resulted in maximum biomass and lipid productivity (mgL(-1)day(-1)) of 455.5 and 111.85, respectively; (iii) single-stage mixotrophic fed-batch cultivation showed maximum biomass productivity of 1.93gL(-1)day(-1) (biomass titer 15.81gL(-1)) and lipid productivity of 550mgL(-1)day(-1); (iv) biodiesel properties were in accordance with international standards.
authors.affiliation: Centre for Energy, Indian Institute of Technology, Guwahati, Assam 781039, India.
authors.forename: Vikram
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Guwahati, Assam 781039, India.
authors.forename: Muthusivaramapandian
authors.initials: M
authors.lastname: Muthuraj
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Guwahati, Assam 781039, India.
authors.forename: Basavaraj
authors.initials: B
authors.lastname: Palabhanvi
authors.affiliation: Centre for Energy, Indian Institute of Technology, Guwahati, Assam 781039, India.
authors.forename: Aloke Kumar
authors.initials: AK
authors.lastname: Ghoshal
authors.affiliation: Centre for Energy, Indian Institute of Technology, Guwahati, Assam 781039, India; Department of Biotechnology, Indian Institute of Technology, Guwahati, Assam 781039, India. Electronic address: debasishd@iitg.ernet.in.
authors.forename: Debasish
authors.initials: D
authors.lastname: Das
mesh_terms: D000085:Acetates / Q000378:metabolism; D056804:Biofuels; D018533:Biomass; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism; D002708:Chlorella / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D050356:Lipid Metabolism / Q000502:physiology*; D058086:Microalgae / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
chemical_list: D000085:Acetates; D056804:Biofuels; D002244:Carbon; D005947:Glucose; D009584:Nitrogen
keywords: Biodiesel; Chlorella; Fed-batch; Mixotrophic; Photoautotrophic