title: QTug.sau-3B is a major quantitative trait locus for wheat hexaploidization.
abstract: Meiotic nonreduction resulting in unreduced gametes is thought to be the predominant mechanism underlying allopolyploid formation in plants. Until now, however, its genetic base was largely unknown. The allohexaploid crop common wheat (Triticum aestivum L.), which originated from hybrids of T. turgidum L. with Aegilops tauschii Cosson, provides a model to address this issue. Our observations of meiosis in pollen mother cells from T. turgidum×Ae. tauschii hybrids indicated that first division restitution, which exhibited prolonged cell division during meiosis I, was responsible for unreduced gamete formation. A major quantitative trait locus (QTL) for this trait, named QTug.sau-3B, was detected on chromosome 3B in two T. turgidum×Ae. tauschii haploid populations. This QTL is situated between markers Xgwm285 and Xcfp1012 and covered a genetic distance of 1 cM in one population. QTug.sau-3B is a haploid-dependent QTL because it was not detected in doubled haploid populations. Comparative genome analysis indicated that this QTL was close to Ttam-3B, a collinear homolog of tam in wheat. Although the relationship between QTug.sau-3B and Ttam requires further study, high frequencies of unreduced gametes may be related to reduced expression of Ttam in wheat.
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan 611130, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Hao
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan 611130, People's Republic of China.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Luo
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan 611130, People's Republic of China.
authors.forename: Deying
authors.initials: D
authors.lastname: Zeng
authors.affiliation: Institute of Ecological Forestry, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan 611130, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan 611130, People's Republic of China.
authors.forename: Shunzong
authors.initials: S
authors.lastname: Ning
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan 611130, People's Republic of China.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan 611130, People's Republic of China.
authors.forename: Zehong
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, People's Republic of China.
authors.forename: Huaigang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan 611130, People's Republic of China.
authors.forename: Youliang
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: INRA University Blaise Pascal, Joint Research Unit 1095 Genetics Diversity and Ecophysiology of Cereals, Clermont-Ferrand 63039, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Feuillet
authors.affiliation: INRA University Blaise Pascal, Joint Research Unit 1095 Genetics Diversity and Ecophysiology of Cereals, Clermont-Ferrand 63039, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Choulet
authors.affiliation: Department of Biology and Microbiology, South Dakota State University, Brookings, South Dakota 57007.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yen
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan 611130, People's Republic of China dcliu7@yahoo.com zhanglianquan1977@126.com.
authors.forename: Lianquan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan 611130, People's Republic of China Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, People's Republic of China dcliu7@yahoo.com zhanglianquan1977@126.com.
authors.forename: Dengcai
authors.initials: D
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D055028:Comparative Genomic Hybridization; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism; D016213:Cyclins / Q000235:genetics / Q000378:metabolism; D008540:Meiosis; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011123:Polyploidy; D040641:Quantitative Trait Loci; D016415:Sequence Alignment; D014908:Triticum / Q000235:genetics*
chemical_list: D016213:Cyclins; D010940:Plant Proteins; D018844:Cyclin-Dependent Kinases
keywords: CYCA1;2/TAM; Triticum aestivum; allopolyploidy; first division restitution; unreduced gametes