title: Tel1ATM dictates the replication timing of short yeast telomeres.
abstract: Telomerase action is temporally linked to DNA replication. Although yeast telomeres are normally late replicating, telomere shortening leads to early firing of subtelomeric DNA replication origins. We show that double-strand breaks flanked by short telomeric arrays cause origin firing early in S phase at late-replicating loci and that this effect on origin firing time is dependent on the Tel1(ATM) checkpoint kinase. The effect of Tel1(ATM) on telomere replication timing extends to endogenous telomeres and is stronger than that elicited by Rif1 loss. These results establish that Tel1(ATM) specifies not only the extent but also the timing of telomerase recruitment.
authors.affiliation: Genome Damage and Stability Centre, School of Life Sciences University of Sussex, Brighton, UK.
authors.forename: Carol
authors.initials: C
authors.lastname: Cooley
authors.affiliation: Genome Damage and Stability Centre, School of Life Sciences University of Sussex, Brighton, UK.
authors.forename: Anoushka
authors.initials: A
authors.lastname: Davé
authors.affiliation: Genome Damage and Stability Centre, School of Life Sciences University of Sussex, Brighton, UK.
authors.forename: Mansi
authors.initials: M
authors.lastname: Garg
authors.affiliation: Genome Damage and Stability Centre, School of Life Sciences University of Sussex, Brighton, UK A.Bianchi@sussex.ac.uk.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Bianchi
mesh_terms: D053903:DNA Breaks, Double-Stranded; D004261:DNA Replication / Q000235:genetics*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D018741:Replication Origin / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D016196:S Phase / Q000235:genetics; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D019098:Telomerase / Q000235:genetics; D016615:Telomere / Q000235:genetics*; D059506:Telomere Shortening / Q000235:genetics*; D034501:Telomere-Binding Proteins / Q000235:genetics
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D034501:Telomere-Binding Proteins; C074461:RIF1 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases; C513623:TEL1 protein, S cerevisiae; D019098:Telomerase
keywords: DNA replication; Tel1; origin firing; replication timing; telomeres