title: Expression of perilipin 2 (PLIN2) in porcine oocytes during maturation.
abstract: Perilipins have been reported to limit the interaction of lipases with neutral lipids within the droplets, thereby regulating neutral lipid accumulation and utilization. This study aimed to identify the location and expression of PLIN1 and PLIN2 in porcine oocytes during maturation. Quantitative real-time polymerase chain reaction (qRT-PCR), immunostaining and Western blot methods were used to characterize the expression and distribution patterns of PLIN1 and PLIN2 in porcine oocytes. The results showed that PLIN1 was not detectable in porcine oocytes. PLIN2 and BODIPY 493/503-detected neutral lipid droplets appeared identical distribution patterns and extensive colocalization in both GV and MII porcine oocytes. PLIN2 protein expression was higher in GV oocytes than that in MII oocytes (p < 0.05), although PLIN2 mRNA expression was similar in both groups. These findings suggested that PLIN2 was a major lipid droplet-associated protein in porcine oocytes.
authors.affiliation: National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: R N
authors.initials: RN
authors.lastname: Zhang
authors.forename: X W
authors.initials: XW
authors.lastname: Fu
authors.forename: B Y
authors.initials: BY
authors.lastname: Jia
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: K R
authors.initials: KR
authors.lastname: Cheng
authors.forename: S E
authors.initials: SE
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009865:Oocytes / Q000502:physiology*; D000070778:Perilipin-1; D000070780:Perilipin-2; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D013552:Swine / Q000502:physiology*
chemical_list: C527198:4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D002352:Carrier Proteins; D008565:Membrane Proteins; D000070778:Perilipin-1; D000070780:Perilipin-2; D010750:Phosphoproteins