title: Isolation, characterization and expression analysis of the BABY BOOM (BBM) gene from Larix kaempferi × L. olgensis during adventitious rooting.
abstract: The full-length cDNA and genomic sequences of the BABY BOOM (BBM) gene, designated LkBBM, were isolated from Larix kaempferi × Larix olgensis. The 3324 bp cDNA was cloned and its open reading frame (ORF) consists of 2370 nucleotides. The deduced 789 amino acid protein contains two AP2 domains and a BBM specific motif. Four conserved motifs between BBM and PLT were identified, which may be conducive to the similar function of BBM and PLT. The three dimensional (3D) structure of LkBBM was predicted and β-sheets in the AP2-R2 domain of LkBBM might recognize the specific base pairs in the major groove. Analysis of the LkBBM gene structure indicates that the gene has eight introns and nine exons. In the 5'-flanking promoter region of LkBBM, many important potential cis-acting elements were identified, such as the TATABOX5 element (a functional TATA element), ROOTMOTIFTAPOX1 element (element of root specificity), AUXREPSIAA4 element (element involved in auxin responsiveness and gene expression in root meristem), MYB1AT element (element involved in MYB recognition), ARR1AT element (element involved in cytokinin responsiveness), GARE1OSREP1 element (element involved in gibberellin responsiveness) and PYRIMIDINEBOXHVEPB1 element (element involved in abscisic acid responsiveness), which all suggested that the expression of LkBBM is highly regulated. Compared with gene expression levels in the stem, stem tip and leaf, LkBBM shows a specific expression in the root, which indicates that LkBBM plays a key role in regulating the development and growth of root in larch. In the processing of larch adventitious root formation, LkBBM started to express on the eighth day after rooting treatment and its transcript level increased continuously afterwards. According to the gene characteristics, LkBBM is proposed as a molecular marker for root primordia of larch, and the initial period of LkBBM expression may be the formation period of root primordia in the processing of adventitious rooting of larch.
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Xiangshan Rd., Beijing 100091, PR China; Research Institute of Forestry, Chinese Academy of Forestry, Xiangshan Rd., Beijing 100091, PR China.
authors.forename: Kui-Peng
authors.initials: KP
authors.lastname: Li
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Xiangshan Rd., Beijing 100091, PR China; Research Institute of Forestry, Chinese Academy of Forestry, Xiangshan Rd., Beijing 100091, PR China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Xiangshan Rd., Beijing 100091, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Han
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Xiangshan Rd., Beijing 100091, PR China; Research Institute of Forestry, Chinese Academy of Forestry, Xiangshan Rd., Beijing 100091, PR China. Electronic address: shougong.zhang@caf.ac.cn.
authors.forename: Shou-Gong
authors.initials: SG
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D028221:Larix / Q000235:genetics* / Q000254:growth & development; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics* / Q000254:growth & development; D011401:Promoter Regions, Genetic / Q000235:genetics; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins
keywords: Adventitious rooting; BABY BOOM; Expression pattern; Larix kaempferi×L. olgensis; Motif analysis