title: Intrastrain comparison of the chemical composition and antioxidant activity of an edible mushroom, Pleurotus giganteus, and its potent neuritogenic properties.
abstract: Two strains of Pleurotus giganteus (commercial and wild) were tested for their ability to induce neurite outgrowth in rat pheochromocytoma (PC12) and mouse neuroblastoma-2a (N2a) cells. Treatment with the mushroom extracts resulted in neuronal differentiation and neuronal elongation, but not nerve growth factor (NGF) production. Linoleic acid (4.5-5.0%, w/w) which is a major fatty acid present in the ethanol extract promoted NGF biosynthesis when augmented with low concentration of NGF (5 ng/mL). The two strains of mushroom were found to be high in protein (154-192 g kg(-1)), total polysaccharides, phenolics, and flavonoids as well as vitamins B1, B2, and B3. The total phenolics present in the mushroom extracts were positively correlated to the antioxidant activity (free radical scavenging, ferric reducing power, and lipid peroxidation inhibition). To conclude, P. giganteus could potentially be used in well-balanced diet and as a source of dietary antioxidant to promote neuronal health.
authors.affiliation: Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Chia-Wei
authors.identifier: 0000-0003-1373-1374
authors.initials: CW
authors.lastname: Phan
authors.affiliation: Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Department of Anatomy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Pamela
authors.initials: P
authors.lastname: David
authors.affiliation: Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Yee-Shin
authors.initials: YS
authors.lastname: Tan
authors.affiliation: Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Department of Anatomy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Murali
authors.identifier: 0000-0001-6156-430X
authors.initials: M
authors.lastname: Naidu
authors.affiliation: Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Department of Anatomy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Kah-Hui
authors.initials: KH
authors.lastname: Wong
authors.affiliation: Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Umah Rani
authors.initials: UR
authors.lastname: Kuppusamy
authors.affiliation: Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Vikineswary
authors.initials: V
authors.lastname: Sabaratnam
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000032:analysis; D005419:Flavonoids / Q000032:analysis; D019787:Linoleic Acid; D008296:Malaysia; D051379:Mice; D020932:Nerve Growth Factor / Q000096:biosynthesis; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D010636:Phenols / Q000032:analysis; D020076:Pleurotus / Q000737:chemistry* / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis; D051381:Rats; D014020:Tissue Extracts / Q000032:analysis / Q000494:pharmacology*
chemical_list: D000596:Amino Acids; D000975:Antioxidants; D005227:Fatty Acids; D005419:Flavonoids; D010636:Phenols; D011134:Polysaccharides; D014020:Tissue Extracts; D020932:Nerve Growth Factor; D019787:Linoleic Acid; D001205:Ascorbic Acid