title: Similar differentiation patterns between PBP expression levels and pheromone component ratios in two populations of Sesamia nonagrioides.
abstract: Pheromone-binding proteins (PBPs) are thought to contribute to the specificity of the pheromone detection system through an initial selective binding with pheromone molecules. Here, we report different expression levels of PBP transcripts in the antennae of two populations of the stemborer Sesamia nonagrioides (Lepidoptera: Noctuidae), one collected in Europe and one in sub-Saharan Africa. The three PBP transcripts previously identified in this species were found to be expressed in both male and female antennae. Whereas PBP3 did not show any differential expression, PBP1 and PBP2 appeared to be expressed differently according to the population origin and sex. Simultaneously, we measured and compared the ratio of the three components of the S. nonagrioides pheromone blend (Z11-16:Ac; Z11-16:OH; Z11-16:Ald) in females of the two populations. The ratio of Z11-16:OH and Z11-16:Ald varied significantly according to the population origin of this species. Cluster analyses revealed similar differentiation patterns between PBP1 and PBP2 expression levels and the ratios of Z11-16:OH and Z11-16:Ald. Different female sexual signals may thus correspond to different male reception systems, which are adjusted by the PBP expression levels, thereby ensuring optimal communication within populations.
authors.affiliation: IRD, UR 072, c/o CNRS, Laboratoire Evolution, Génomes et Spéciation, 91198, Gif-sur-Yvette Cedex, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Glaser
authors.forename: Brigitte
authors.initials: B
authors.lastname: Frérot
authors.forename: Ene
authors.initials: E
authors.lastname: Leppik
authors.forename: Christelle
authors.initials: C
authors.lastname: Monsempes
authors.forename: Claire
authors.initials: C
authors.lastname: Capdevielle-Dulac
authors.forename: Bruno
authors.initials: B
authors.lastname: Le Ru
authors.forename: Thomas
authors.initials: T
authors.lastname: Lecocq
authors.forename: Myriam
authors.initials: M
authors.lastname: Harry
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Jacquin-Joly
authors.forename: Paul-Andre
authors.initials: PA
authors.lastname: Calatayud
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D005260:Female; D005602:France; D015870:Gene Expression; D005843:Geography; D019476:Insect Proteins / Q000378:metabolism*; D007630:Kenya; D008297:Male; D009036:Moths / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D012724:Sex Attractants / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D019476:Insect Proteins; D012724:Sex Attractants; C403713:pheromone binding protein, insect