title: Mapping and introgression of QTL involved in fruit shape transgressive segregation into ‘piel de sapo’ melon (cucumis melo l.) [corrected].
abstract: A mapping F2 population from the cross 'Piel de Sapo' × PI124112 was selectively genotyped to study the genetic control of morphological fruit traits by QTL (Quantitative Trait Loci) analysis. Ten QTL were identified, five for FL (Fruit Length), two for FD (Fruit Diameter) and three for FS (Fruit Shape). At least one robust QTL per character was found, flqs8.1 (LOD = 16.85, R2 = 34%), fdqs12.1 (LOD = 3.47, R2 = 11%) and fsqs8.1 (LOD = 14.85, R2 = 41%). flqs2.1 and fsqs2.1 cosegregate with gene a (andromonoecious), responsible for flower sex determination and with pleiotropic effects on FS. They display a positive additive effect (a) value, so the PI124112 allele causes an increase in FL and FS, producing more elongated fruits. Conversely, the negative a value for flqs8.1 and fsqs8.1 indicates a decrease in FL and FS, what results in rounder fruits, even if PI124112 produces very elongated melons. This is explained by a significant epistatic interaction between fsqs2.1 and fsqs8.1, where the effects of the alleles at locus a are attenuated by the additive PI124112 allele at fsqs8.1. Roundest fruits are produced by homozygous for PI124112 at fsqs8.1 that do not carry any dominant A allele at locus a (PiPiaa). A significant interaction between fsqs8.1 and fsqs12.1 was also detected, with the alleles at fsqs12.1 producing more elongated fruits. fsqs8.1 seems to be allelic to QTL discovered in other populations where the exotic alleles produce elongated fruits. This model has been validated in assays with backcross lines along 3 years and ultimately obtaining a fsqs8.1-NIL (Near Isogenic Line) in 'Piel de Sapo' background which yields round melons.
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (IBMCP). Universidad Politécnica de Valencia (UPV)-Consejo Superior de Investigaciones Científicas (CSIC), Valencia, Spain.
authors.forename: Aurora
authors.initials: A
authors.lastname: Díaz
authors.affiliation: Laboratorio de Biología Molecular. Dpto. Investigación Agroalimentaria. Instituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario (IMIDRA), Alcalá de Henares, Madrid, Spain.
authors.forename: Belkacem
authors.initials: B
authors.lastname: Zarouri
authors.affiliation: Centre de Recerca en Agrigenòmica (CRAG), IRTA-CSIC-UAB, Bellaterra, Cerdanyola del Vallès, Barcelona, Spain.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Fergany
authors.affiliation: Centre de Recerca en Agrigenòmica (CRAG), IRTA-CSIC-UAB, Bellaterra, Cerdanyola del Vallès, Barcelona, Spain.
authors.forename: Iban
authors.initials: I
authors.lastname: Eduardo
authors.affiliation: Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Zaragoza, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Alvarez
authors.affiliation: COMAV-UPV, Institute for the Conservation and Breeding of Agricultural Biodiversity, Universitad Politécnica de Valencia, Valencia, Spain.
authors.forename: Belén
authors.initials: B
authors.lastname: Picó
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (IBMCP). Universidad Politécnica de Valencia (UPV)-Consejo Superior de Investigaciones Científicas (CSIC), Valencia, Spain.
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: Monforte
mesh_terms: D000704:Analysis of Variance; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D028463:Cucumis melo / Q000235:genetics*; D004843:Epistasis, Genetic; D005638:Fruit / Q000235:genetics*; D008040:Genetic Linkage; D010641:Phenotype; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable; D015203:Reproducibility of Results