title: A novel restricted access material combined to molecularly imprinted polymers for selective solid-phase extraction and high performance liquid chromatography determination of 2-methoxyestradiol in plasma samples.
abstract: A feasibility study was performed in order to ensure the possibilities in using a restricted access material combined to molecularly imprinted polymers (RAM-MIP) as sorbent material in solid phase extraction (SPE) for clean-up of 2-methoxyestradiol (2-ME) from plasma samples. The MIP with hydrophilic external layer was designed by precipitation polymerization. The polymer was characterized by thermogravimetric analysis (TGA) and scanning electron microscope (SEM). The use of analogs of 2-ME as templates, in combination with a chromatographic separation of the analytes in the sample, overcame the problem of the template bleeding. To demonstrate the property of the RAM-MIP obtained, a comparison of commercially available C18 SPE was performed. The results showed that the RAM-MISPE recoveries were significantly higher than that of C18 SPE for 2-ME in trace concentration. During the extraction process, 2-ME was sufficiently cleaned for further chromatographic analysis with no interferences from template leakage and matrix. Good linearity was obtained from 0.06 to 20 μg mL(-1) with the correlation coefficient r>0.9991. The coefficient of variation of the inter-assay precision was less than 11.9%. The recoveries of 2-ME in rat plasma at three spiked levels were in the range of 99.10-101.00%. Based on the analytical validation results, the proposed method (RAM-MIP off-line SPE/HPLC) can be a useful tool to determine 2-ME in rat plasma samples.
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Science Road 100, Zhengzhou 450001, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Science Road 100, Zhengzhou 450001, PR China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Qu
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Science Road 100, Zhengzhou 450001, PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Chen
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Science Road 100, Zhengzhou 450001, PR China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Cao
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Science Road 100, Zhengzhou 450001, PR China.
authors.forename: Shuping
authors.initials: S
authors.lastname: Han
authors.affiliation: School of Chemical Engineering and Energy, Zhengzhou University, Science Road 100, Zhengzhou 450001, PR China. Electronic address: sgppaper@sina.com.
authors.forename: Guopeng
authors.initials: G
authors.lastname: Shen
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Science Road 100, Zhengzhou 450001, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D000077584:2-Methoxyestradiol; D000327:Adsorption; D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004852:Epoxy Compounds / Q000737:chemistry*; D004958:Estradiol / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry; D005240:Feasibility Studies; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods; D013499:Surface Properties; D013696:Temperature; D013818:Thermogravimetry / Q000379:methods
chemical_list: D004852:Epoxy Compounds; D008689:Methacrylates; D011108:Polymers; D004958:Estradiol; D000077584:2-Methoxyestradiol; C007870:glycidyl methacrylate
keywords: 2-Methoxyestradiol; High performance liquid chromatography; Molecular imprinted polymers; Restricted access matrix; Solid phase extraction