title: Neurolaena lobata L. promotes wound healing in Sprague Dawley rats.
abstract: BACKGROUND
  The leaves of the Neurolaena lobata (Asteraceae) plant are used to control diabetes and heal wounds and infections.
  
  
  OBJECTIVE
  The ethanolic extract of N. lobata leaf was evaluated for its ability to heal inflicted wounds in rats using the excision wound model.
  
  
  METHODS
  Animals were divided into three groups of six each. Test group animals were treated topically with an ethanolic extract of N. lobata (1:1 with petroleum jelly, 100 mg/kg/day). Standard and control group animals were treated with mupirocin and petroleum jelly, respectively. Treatment was given for 13 days and the wound area was measured on alternate days. Parameters of healing assessed were the rate of wound contraction, period of epithelialization and hydroxyproline content. Antimicrobial activity of the extract was observed against Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli.
  
  
  RESULTS
  Phytochemical analysis of the extract showed the presence of saponins, tannins, alkaloids and flavanoids. Extract-treated animals exhibited 87% reduction in the wound area over 13 days when compared with the control (78%) and standard (83%) groups (P < 0.05). A significant decrease in the epithelialization period was noticed with the extract-treated test group animals compared with the controls and the standard group animals (P < 0.008). The hydroxyproline content of the extract-treated animals was higher (230.5 ± 42.1) when evaluated against the control and (79.0 ± 32.2) and the standard (115.0 ± 44.5) groups (P < 0.05).
  
  
  CONCLUSIONS
  Increase in the rate of wound contraction and hydroxyproline content with decrease in epithelialization time in extract-treated animals support further evaluation of N. lobata as a pharmacotherapy for wound healing.
authors.affiliation: Department of Preclinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.
authors.forename: Bijoor Shivananda
authors.initials: BS
authors.lastname: Nayak
authors.affiliation: Department of Preclinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.
authors.forename: Surrin
authors.initials: S
authors.lastname: Ramlogan
authors.affiliation: Department of Paraclinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.
authors.forename: Av
authors.initials: A
authors.lastname: Chalapathi Rao
authors.affiliation: Department of School of Pharmacy, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Maharaj
keywords: Antimicrobial activity; Neurolaena lobata; epithelialization; hydroxyproline