title: High-performance lectin affinity chromatography.
abstract: Lectin high-performance liquid chromatography techniques have contributed to the growing interest in glycoproteomics. Affinity chromatography is a very effective method to separate and purify trace amount of biological substances. In this chapter, we describe a basic procedure for separation of glycoproteins using commercially available lectin-HPLC columns. As an example, α-fetoprotein, known as a biomarker of liver cancer, can be separated at the level of their glyco-isomers by using a Lens culinaris agglutinin (LCA) column.
authors.affiliation: J-Oil Mills, Inc., 11, Kagetoricho, Totsuka-ku, Yokohama, Kanagawa, 245-0064, Japan, yuka.kobayashi@j-oil.com.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kobayashi
mesh_terms: D053000:Analytic Sample Preparation Methods; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D037121:Plant Lectins / Q000378:metabolism*
chemical_list: D037121:Plant Lectins