title: An increasing opine carbon bias in artificial exudation systems and genetically modified plant rhizospheres leads to an increasing reshaping of bacterial populations.
abstract: To investigate how exudation shapes root-associated bacterial populations, transgenic Arabidopsis thaliana plants that exuded the xenotopic compound octopine at low and high rates were grown in a nonsterile soil. Enumerations of both cultivable and octopine-degrading bacteria demonstrated that the ratios of octopine degraders increased along with octopine concentration. An artificial exudation system was also set up in which octopine was brought at four ratios. The density of octopine-degrading bacteria directly correlated with the input of octopine. Bacterial diversity was analysed by rrs amplicon pyrosequencing. Ensifer and Pseudomonas were significantly more frequently detected in soil amended with artificial exudates. However, the density of Pseudomonas increased as a response to carbon supplementation while that of Ensifer only correlated with octopine concentrations possibly in relation to two opposed colonization strategies of rhizosphere bacteria, that is, copiotrophy and oligotrophy.
authors.affiliation: Institut des Sciences du Végétal (ISV), UPR2355, CNRS, Saclay Plant Sciences, Avenue de la Terrasse, Gif-sur-Yvette, 91198, France.
authors.forename: Samuel
authors.initials: S
authors.lastname: Mondy
authors.forename: Aurore
authors.initials: A
authors.lastname: Lenglet
authors.forename: Amelie
authors.initials: A
authors.lastname: Beury-Cirou
authors.forename: Celestin
authors.initials: C
authors.lastname: Libanga
authors.forename: Pascal
authors.initials: P
authors.lastname: Ratet
authors.forename: Denis
authors.initials: D
authors.lastname: Faure
authors.forename: Yves
authors.initials: Y
authors.lastname: Dessaux
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000737:chemistry* / Q000382:microbiology; D001120:Arginine / Q000031:analogs & derivatives / Q000737:chemistry; D001419:Bacteria / Q000302:isolation & purification; D044822:Biodiversity; D002244:Carbon / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D053147:Plant Exudates / Q000737:chemistry*; D018517:Plant Roots / Q000382:microbiology*; D030821:Plants, Genetically Modified / Q000737:chemistry; D058441:Rhizosphere; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D053147:Plant Exudates; C005394:octopine; D002244:Carbon; D001120:Arginine
keywords: copiotrophy; deseq; genetically modified plants; oligotrophy; opine; pyrosequencing; root exudates