title: Development of optimal medium content for bioelements accumulation in Bacopa monnieri (L.) in vitro culture.
abstract: Bacopa monnieri is one of the most interesting plants from the Ayurveda system. The aims of present research were, basing on in vitro shoot culture of B. monnieri, to determine content and to evaluate the influence of physiologically important metabolites on the selected bioelements accumulation in biomass. The most significant increase in biomass production was observed in the culture medium enriched with 0.5 mg/L of anthranilic acid. In this medium also, the highest accumulation of Mg was noted. The highest concentration of iron was determined in B. monnieri in vitro culture enriched with 0.25 g/L of serine. The addition of L-tryptophan, magnesium sulfate, and zinc hydroaspartate caused only a small increase in the accumulation of copper in B. monnieri. Increase in Zn accumulation was obtained in biomass from in vitro culture of B. monnieri with the addition of magnesium sulfate and zinc hydroaspartate. In the case of Na, the maximum level of this element was in biomass from medium enriched with zinc hydroaspartate. Twofold increase in K concentration was obtained in biomass from cultures on medium with addition of serine and magnesium sulfate. The concentrations of Ca in biomass of all studied media were at the similar level.
authors.affiliation: Department of Pharmaceutical Botany, Jagiellonian University Collegium Medicum, ul. Medyczna 9, 30-688, Kraków, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Łojewski
authors.affiliation: Department of Pharmaceutical Botany, Jagiellonian University Collegium Medicum, ul. Medyczna 9, 30-688, Kraków, Poland. muchon@poczta.fm.
authors.forename: Bożena
authors.initials: B
authors.lastname: Muszyńska
authors.affiliation: Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Kraków, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Smalec
authors.affiliation: Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Kraków, Poland.
authors.forename: Witold
authors.initials: W
authors.lastname: Reczyński
authors.affiliation: Department of Inorganic and Analytical Chemistry, Jagiellonian University Collegium Medicum, ul. Medyczna 9, 30-688, Kraków, Poland.
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Opoka
authors.affiliation: Department of Pharmaceutical Botany, Jagiellonian University Collegium Medicum, ul. Medyczna 9, 30-688, Kraków, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Sułkowska-Ziaja
mesh_terms: D034681:Bacopa / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology; D008670:Metals / Q000737:chemistry / Q000494:pharmacology; D018520:Plant Shoots / Q000254:growth & development*; D062367:ortho-Aminobenzoates / Q000737:chemistry / Q000494:pharmacology
chemical_list: D003470:Culture Media; D008670:Metals; D062367:ortho-Aminobenzoates; C031385:anthranilic acid