title: Resveratrol modulates the angiogenic response to exercise training in skeletal muscles of aged men.
abstract: In animal studies, the polyphenol resveratrol has been shown to influence several pathways of importance for angiogenesis in skeletal muscle. The aim of the present study was to examine the angiogenic effect of resveratrol supplementation with parallel exercise training in aged men. Forty-three healthy physically inactive aged men (65 ± 1 yr) were divided into 1) a training group that conducted 8 wk of intense exercise training where half of the subjects received a daily intake of either 250 mg trans-resveratrol (n = 14) and the other half received placebo (n = 13) and 2) a nontraining group that received either 250 mg trans-resveratrol (n = 9) or placebo (n = 7). The group that trained with placebo showed a ~20% increase in the capillary-to-fiber ratio, an increase in muscle protein expression of VEGF, VEGF receptor-2, and tissue inhibitor of matrix metalloproteinase (TIMP-1) but unaltered thrombospodin-1 levels. Muscle interstitial VEGF and thrombospodin-1 protein levels were unchanged after the training period. The group that trained with resveratrol supplementation did not show an increase in the capillary-to-fiber ratio or an increase in muscle VEGF protein. Muscle TIMP-1 protein levels were lower in the training and resveratrol group than in the training and placebo group. Both training groups showed an increase in forkhead box O1 protein. In nontraining groups, TIMP-1 protein was lower in the resveratrol-treated group than the placebo-treated group after 8 wk. In conclusion, these data show that exercise training has a strong angiogenic effect, whereas resveratrol supplementation may limit basal and training-induced angiogenesis.
authors.affiliation: Integrative Physiology Group, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark; and gliemann@nexs.ku.dk.
authors.forename: Lasse
authors.initials: L
authors.lastname: Gliemann
authors.affiliation: Centre of Inflammation and Metabolism, Department of Biology, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Jesper
authors.initials: J
authors.lastname: Olesen
authors.affiliation: Centre of Inflammation and Metabolism, Department of Biology, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Rasmus Sjørup
authors.initials: RS
authors.lastname: Biensø
authors.affiliation: Integrative Physiology Group, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark; and.
authors.forename: Jakob Friis
authors.initials: JF
authors.lastname: Schmidt
authors.affiliation: Integrative Physiology Group, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark; and.
authors.forename: Thorbjorn
authors.initials: T
authors.lastname: Akerstrom
authors.affiliation: Integrative Physiology Group, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark; and.
authors.forename: Michael
authors.initials: M
authors.lastname: Nyberg
authors.affiliation: Integrative Physiology Group, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark; and.
authors.forename: Anna
authors.initials: A
authors.lastname: Lindqvist
authors.affiliation: Integrative Physiology Group, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark; and.
authors.forename: Jens
authors.initials: J
authors.lastname: Bangsbo
authors.affiliation: Integrative Physiology Group, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark; and.
authors.forename: Ylva
authors.initials: Y
authors.lastname: Hellsten
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D019587:Dietary Supplements; D015444:Exercise; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D018919:Neovascularization, Physiologic; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage / Q000494:pharmacology*; D019700:Thrombospondin 1 / Q000235:genetics / Q000378:metabolism; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics / Q000378:metabolism
chemical_list: C085126:FOXO1 protein, human; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; D013267:Stilbenes; C560822:TIMP1 protein, human; D019700:Thrombospondin 1; D019715:Tissue Inhibitor of Metalloproteinase-1; D042461:Vascular Endothelial Growth Factor A; C000588866:KDR protein, human; D040301:Vascular Endothelial Growth Factor Receptor-2; D000077185:Resveratrol
keywords: aging; antioxidant; capillary; physical activity; vascular endothelial growth factor