title: High potential source for biomass degradation enzyme discovery and environmental aspects revealed through metagenomics of Indian buffalo rumen.
abstract: The complex microbiomes of the rumen functions as an effective system for plant cell wall degradation, and biomass utilization provide genetic resource for degrading microbial enzymes that could be used in the production of biofuel. Therefore the buffalo rumen microbiota was surveyed using shot gun sequencing. This metagenomic sequencing generated 3.9 GB of sequences and data were assembled into 137270 contiguous sequences (contigs). We identified potential 2614 contigs encoding biomass degrading enzymes including glycoside hydrolases (GH: 1943 contigs), carbohydrate binding module (CBM: 23 contigs), glycosyl transferase (GT: 373 contigs), carbohydrate esterases (CE: 259 contigs), and polysaccharide lyases (PE: 16 contigs). The hierarchical clustering of buffalo metagenomes demonstrated the similarities and dissimilarity in microbial community structures and functional capacity. This demonstrates that buffalo rumen microbiome was considerably enriched in functional genes involved in polysaccharide degradation with great prospects to obtain new molecules that may be applied in the biofuel industry.
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat 388001, India ; Xcelris Genomics Xcelris Labs Ltd, Old Premchandnagar Road, Opp. Satyagrah Chhavani, Bodakdev, Ahmedabad 380054, India.
authors.forename: K M
authors.initials: KM
authors.lastname: Singh
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat 388001, India.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Reddy
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat 388001, India.
authors.forename: Dishita
authors.initials: D
authors.lastname: Patel
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat 388001, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Patel
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat 388001, India.
authors.forename: Nidhi
authors.identifier: 0000-0002-3814-0629
authors.initials: N
authors.lastname: Parmar
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat 388001, India.
authors.forename: Anand
authors.identifier: 0000-0003-0109-4128
authors.initials: A
authors.lastname: Patel
authors.affiliation: Department of Animal Nutrition, Livestock Research Station, Sardarkrushinagar Dantiwada Agricultural University, Gujarat 385506, India.
authors.forename: J B
authors.initials: JB
authors.lastname: Patel
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat 388001, India.
authors.forename: C G
authors.initials: CG
authors.lastname: Joshi
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018533:Biomass; D002020:Buffaloes / Q000382:microbiology*; D054892:Metagenome / Q000502:physiology*; D064307:Microbiota / Q000502:physiology*; D012417:Rumen / Q000201:enzymology / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins