title: Comparison of saponin composition and content in wild soybean (Glycine soja Sieb. and Zucc.) before and after germination.
abstract: Eight wild soybean accessions with different saponin phenotypes were used to examine saponin composition and relative saponin quantity in various tissues of mature seeds and two-week-old seedlings by LC-PDA/MS/MS. Saponin composition and content were varied according to tissues and accessions. The average total saponin concentration in 1 g mature dry seeds of wild soybean was 16.08 ± 3.13 μmol. In two-week-old seedlings, produced from 1 g mature seeds, it was 27.94 ± 6.52 μmol. Group A saponins were highly concentrated in seed hypocotyl (4.04 ± 0.71 μmol). High concentration of DDMP saponins (7.37 ± 5.22 μmol) and Sg-6 saponins (2.19 ± 0.59 μmol) was found in cotyledonary leaf. In seedlings, the amounts of group A and Sg-6 saponins reduced 2.3- and 1.3-folds, respectively, while DDMP + B + E saponins increased 2.5-fold than those of mature seeds. Our findings show that the group A and Sg-6 saponins in mature seeds were degraded and/or translocated by germination whereas DDMP saponins were newly synthesized.
authors.affiliation: a Department of Biotechnology , Chonnam National University , Yeosu , Korea.
authors.forename: Panneerselvam
authors.initials: P
authors.lastname: Krishnamurthy
authors.forename: Chigen
authors.initials: C
authors.lastname: Tsukamoto
authors.forename: Yuya
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hongo
authors.forename: Ram J
authors.initials: RJ
authors.lastname: Singh
authors.forename: Jeong Dong
authors.initials: JD
authors.lastname: Lee
authors.forename: Gyuhwa
authors.initials: G
authors.lastname: Chung
mesh_terms: D018525:Germination; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification; D036226:Seedlings / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D012503:Saponins
keywords: Glycine soja; germination; soyasaponins; triterpene glycosides; wild soybean