title: Timing the ischaemic stroke by 1H-MRI: improved accuracy using absolute relaxation times over signal intensities.
abstract: One in four ischaemic stroke patients are ineligible for thrombolytic treatment due to unknown onset time. Quantification of absolute MR relaxation times and signal intensities are potential methods for estimating stroke duration. We compared the accuracy of these approaches and determined whether changes in relaxation times and signal intensities identify the same ischaemic tissue as diffusion MRI. Seven Wistar rats underwent permanent middle cerebral artery occlusion to induce focal ischaemia and were scanned at six time points. The trace of the diffusion tensor (DAV), T1ρ and T2 were acquired at 4.7 T. Results show relaxation times, and signal intensities of the MR relaxation parameters increase linearly with ischaemia duration (P<0.001). Using T1ρ and T2 relaxation times, an estimate of 4.5 h after occlusion has an uncertainty of ± 12 and ± 35 min, respectively, compared with over 50 min for signal intensities. In addition, we present a pixel-by-pixel method that simultaneously estimates stroke onset time and identifies potentially irreversible ischaemic tissue using absolute relaxation times. This method demonstrates signal intensity changes during ischaemia display an ambiguous pattern and highlights the possibility that diffusion MRI overestimates the true extent of irreversible ischaemia. In conclusion, quantification of absolute relaxation times at a single time point enables a more accurate estimation of stroke duration than signal intensities and provides more information about tissue status in ischaemia.
authors.affiliation: aSchool of Experimental Psychology and Bristol Clinical Research and Imaging Centre, University of Bristol, Bristol, UK bDepartment of Neurobiology, A.I. Virtanen Institute, University of Eastern Finland, Kuopio, Finland.
authors.forename: Harriet J
authors.initials: HJ
authors.lastname: Rogers
authors.forename: Bryony L
authors.initials: BL
authors.lastname: McGarry
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Knight
authors.forename: Kimmo T
authors.initials: KT
authors.lastname: Jokivarsi
authors.forename: Olli H J
authors.initials: OH
authors.lastname: Gröhn
authors.forename: Risto A
authors.initials: RA
authors.lastname: Kauppinen
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology*; D002545:Brain Ischemia / Q000175:diagnosis* / Q000473:pathology*; D056324:Diffusion Tensor Imaging / Q000379:methods; D004195:Disease Models, Animal; D007839:Functional Laterality; D020244:Infarction, Middle Cerebral Artery; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D011522:Protons; D017208:Rats, Wistar; D012815:Signal Processing, Computer-Assisted; D020521:Stroke / Q000175:diagnosis* / Q000473:pathology*; D061665:Time-to-Treatment
chemical_list: D011522:Protons