title: High-NaCl perception in Drosophila melanogaster.
abstract: Salt is a fundamental nutrient that is required for many physiological processes, including electrolyte homeostasis and neuronal activity. In mammals and Drosophila, the detection of NaCl induces two different behaviors: low-salt concentrations provide an attractive stimulus, whereas high-salt concentrations are avoided. We identified the gene called serrano (sano) as being expressed in the sensory organs of Drosophila larvae. A transgenic reporter line showed that sano was coexpressed with Gr66a in a subset of gustatory neurons in the terminal organ of third-instar larvae. The disruption of sano gene expression in gustatory neurons led to the specific loss of high-salt concentration avoidance in larvae, whereas the detection of other attractive or aversive substances was unaffected. Moreover, using a cellular marker sensitive to calcium levels, Sano function was shown to be required for neuronal activity in response to high-salt concentrations. In these neurons, the loss of the DEG/ENaC channel PPK19 function also eliminated the cellular response to high-salt concentrations. Our study revealed that PPK19 and Sano are required in the neurons of the larval gustatory organs for the detection of high-salt concentrations.
authors.affiliation: Centre des Sciences du Goût et de l'Alimentation, UMR-6265 CNRS, UMR-1324 INRA, Université de Bourgogne, 21000 Dijon, France georges.alves@u-bourgogne.fr.
authors.forename: Georges
authors.identifier: http://orcid.org/0000-0002-5548-0270
authors.initials: G
authors.lastname: Alves
authors.affiliation: Centre des Sciences du Goût et de l'Alimentation, UMR-6265 CNRS, UMR-1324 INRA, Université de Bourgogne, 21000 Dijon, France.
authors.forename: Jérémy
authors.initials: J
authors.lastname: Sallé
authors.affiliation: Centre des Sciences du Goût et de l'Alimentation, UMR-6265 CNRS, UMR-1324 INRA, Université de Bourgogne, 21000 Dijon, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Chaudy
authors.affiliation: Centre des Sciences du Goût et de l'Alimentation, UMR-6265 CNRS, UMR-1324 INRA, Université de Bourgogne, 21000 Dijon, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Dupas
authors.affiliation: Centre des Sciences du Goût et de l'Alimentation, UMR-6265 CNRS, UMR-1324 INRA, Université de Bourgogne, 21000 Dijon, France.
authors.forename: Gérard
authors.initials: G
authors.lastname: Manière
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster; D007814:Larva / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D012965:Sodium Chloride; D013649:Taste / Q000502:physiology*; D055697:Taste Perception / Q000502:physiology*
chemical_list: D002352:Carrier Proteins; D029721:Drosophila Proteins; C548446:sano protein, Drosophila; D012965:Sodium Chloride; D002118:Calcium
keywords: Drosophila melanogaster; behavior; chemosensory system; larva; salt; taste