title: Quercetin glycosides induced neuroprotection by changes in the gene expression in a cellular model of Parkinson's disease.
abstract: Quercetin glycosides, rutin and isoquercitrin, are potent antioxidants that have been found to possess neuroprotective effect in diseases like Parkinson's and Alzheimer's disease. In the present study, we have examined the gene expression changes with rutin and isoquercitrin pretreatment on 6-hydroxydopamine (6-OHDA)-treated toxicity in rat pheochromocytoma (PC12) cells. PC12 cells were pretreated with rutin or isoquercitrin and subsequently exposed to 6-OHDA. Rutin-pretreated PC12 attenuated the Park2, Park5, Park7, Casp3, and Casp7 genes which were expressed significantly in the 6-OHDA-treated PC12 cells. Rutin upregulated the TH gene which is important in dopamine biosynthesis, but isoquercitrin pretreatment did not affect the expression of this gene. Both rutin and isoquercitrin pretreatments upregulated the ion transport and antiapoptotic genes (NSF and Opa1). The qPCR array data were further validated by qRT-PCR using four primers, Park5, Park7, Casp3, and TH. This finding suggests that changes in the expression levels of transcripts encoded by genes that participate in ubiquitin pathway and dopamine biosynthesis may be involved in Parkinson's disease.
authors.affiliation: Department of Pathology, Faculty of Medicine, International Medical University, Kuala Lumpur, Malaysia.
authors.forename: Kasthuri Bai
authors.initials: KB
authors.lastname: Magalingam
authors.forename: Ammu
authors.initials: A
authors.lastname: Radhakrishnan
authors.forename: Premdass
authors.initials: P
authors.lastname: Ramdas
authors.forename: Nagaraja
authors.initials: N
authors.lastname: Haleagrahara
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D006027:Glycosides / Q000494:pharmacology; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D016627:Oxidopamine / Q000633:toxicity; D016716:PC12 Cells; D010300:Parkinson Disease / Q000378:metabolism*; D000071617:Protein Deglycase DJ-1; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D012431:Rutin / Q000494:pharmacology; D043222:Ubiquitin Thiolesterase / Q000235:genetics / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; D006027:Glycosides; D008869:Microtubule-Associated Proteins; D012431:Rutin; D016627:Oxidopamine; D011794:Quercetin; D044767:Ubiquitin-Protein Ligases; C111567:parkin protein; C404267:PARK7 protein, rat; D000071617:Protein Deglycase DJ-1; D043222:Ubiquitin Thiolesterase