title: Urine sample preparation for proteomic analysis.
abstract: Sample preparation for both environmental and more importantly biological matrices is a bottleneck of all kinds of analytical processes. In the case of proteomic analysis this element is even more important due to the amount of cross-reactions that should be taken into consideration. The incorporation of new post-translational modifications, protein hydrolysis, or even its degradation is possible as side effects of proteins sample processing. If protocols are evaluated appropriately, then identification of such proteins does not bring difficulties. However, if structural changes are provided without sufficient attention then protein sequence coverage will be reduced or even identification of such proteins could be impossible. This review summarizes obstacles and achievements in protein sample preparation of urine for proteome analysis using different tools for mass spectrometry analysis. The main aim is to present comprehensively the idea of urine application as a valuable matrix. This article is dedicated to sample preparation and application of urine mainly in novel cancer biomarkers discovery.
authors.affiliation: Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland; Interdisciplinary Centre for Modern Technologies, Nicolaus Copernicus University, Torun, Poland.
authors.forename: Pawel
authors.initials: P
authors.lastname: Olszowy
authors.forename: Boguslaw
authors.initials: B
authors.lastname: Buszewski
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D005612:Freeze Drying; D013058:Mass Spectrometry / Q000379:methods; D011507:Proteinuria / Q000652:urine; D040901:Proteomics; D014461:Ultracentrifugation; D014462:Ultrafiltration; D014556:Urine / Q000737:chemistry*
keywords: Liquid chromatography; Mass spectrometry; Proteomics; Sample preparation; Urine