title: The influence of Th1/Th2 and CD4+ regulatory t cells of mesenteric lymph nodes on systemic lipopolysaccharide.
abstract: Our aims were to study the influence of the mesenteric lymph nodes (MLN) of rats on systemic lipopolysaccharide and to identify the factor that affects the intestinal endotoxin translocation. Ninety-six male Wistar rats were randomly divided into a sham-operation group (S group) and a cecal ligation and perforation group (CLP group). Twenty-four hours after modeling, we tested the Th1/Th2 ratio and percentage of CD4+CD25+Foxp3+ Treg in the MLN. At the same time, the lipopolysaccharide (LPS) in the abdominal aortic blood was detected. In the CLP group, the Th1/Th2 ratio was obviously lower than in the S group. Otherwise, the percentage of CD4+CD25+Foxp3+ Treg of the CLP group was significantly higher than the S group. In the abdominal aortic blood, the LPS level of the CLP group was also higher than in the S group. Through correlation analysis, we found that the level of LPS was positively correlated with the percentage of CD4+CD25+Foxp3+ Treg, and negatively correlated with the Th1/Th2 ratio. This model reveals that the immune suppression of the MLN might affect the level of LPS in the abdominal aortic blood, which might play a certain role in affecting the endotoxin translocation.
authors.affiliation: Hongyin Du Department of Surgical Intensive Care Unit Guangdong Medical University Hospital, PR China tel. +8602082579696 fax +8602082579696 e-mail: hongyindue@163.com.
authors.forename: W
authors.initials: W
authors.lastname: Yu
authors.forename: H
authors.initials: H
authors.lastname: Du
authors.forename: Q
authors.initials: Q
authors.lastname: Fu
authors.forename: N
authors.initials: N
authors.lastname: Cui
authors.forename: X
authors.initials: X
authors.lastname: Du
mesh_terms: D000818:Animals; D018988:Bacterial Translocation; D015415:Biomarkers / Q000097:blood; D002432:Cecum / Q000382:microbiology / Q000601:surgery; D004195:Disease Models, Animal; D051858:Forkhead Transcription Factors / Q000097:blood; D053645:Interleukin-2 Receptor alpha Subunit / Q000097:blood; D008026:Ligation; D008070:Lipopolysaccharides / Q000097:blood*; D008198:Lymph Nodes / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000097:blood / Q000276:immunology* / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D018417:Th1 Cells / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D013997:Time Factors
chemical_list: D015415:Biomarkers; D051858:Forkhead Transcription Factors; C500522:Foxp3 protein, rat; D053645:Interleukin-2 Receptor alpha Subunit; D008070:Lipopolysaccharides