title: Genetic diversity and structure of Dalmatian pyrethrum (Tanacetum cinerariifolium Trevir./Sch./Bip., Asteraceae) within the Balkan refugium.
abstract: Dalmatian pyrethrum (Tanacetum cinerariifolium Trevir./Sch./Bip.) is an outcrossing, perennial insecticidal plant, restricted to the eastern Adriatic coast (Mediterranean). Amplified fragment-length polymorphisms (AFLP) were used to investigate the genetic diversity and structure within and among 20 natural plant populations. The highest level of gene diversity, the number of private alleles and the frequency down-weighted marker values (DW) were found in northern Adriatic populations and gradually decreased towards the southern boundary of the species range. Genetic impoverishment of these southern populations is most likely the result of human-related activities. An analysis of molecular variance (AMOVA) indicated that most of the genetic diversity was attributed to differences among individuals within populations (85.78%), which are expected due to the outcrossing nature of the species. A Bayesian analysis of the population structure identified two dominant genetic clusters. A spatial analysis of the genetic diversity indicated that 5.6% of the genetic differentiation resulted from isolation by distance (IBD), while 12.3% of the genetic differentiation among populations followed the pattern of isolation by environmental distance (IBED). Knowledge of the genetic diversity patterns of the natural populations and the mechanism behind these patterns is required for the exploitation and possible conservation management of this endemic and economically important species.
authors.affiliation: Department of Seed Science and Technology, Faculty of Agriculture, University of Zagreb, Zagreb, Croatia.
authors.forename: Martina
authors.initials: M
authors.lastname: Grdiša
authors.affiliation: Department of Botany, Division of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia.
authors.forename: Zlatko
authors.initials: Z
authors.lastname: Liber
authors.affiliation: Department of Botany, Division of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Radosavljević
authors.affiliation: Department of Seed Science and Technology, Faculty of Agriculture, University of Zagreb, Zagreb, Croatia.
authors.forename: Klaudija
authors.initials: K
authors.lastname: Carović-Stanko
authors.affiliation: Department of Seed Science and Technology, Faculty of Agriculture, University of Zagreb, Zagreb, Croatia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Kolak
authors.affiliation: Department of Seed Science and Technology, Faculty of Agriculture, University of Zagreb, Zagreb, Croatia.
authors.forename: Zlatko
authors.initials: Z
authors.lastname: Satovic
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D063368:Balkan Peninsula; D011723:Chrysanthemum cinerariifolium / Q000145:classification* / Q000235:genetics*; D018744:DNA, Plant; D004777:Environment; D019143:Evolution, Molecular; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D006801:Humans; D010802:Phylogeny
chemical_list: D018744:DNA, Plant