title: Isothermal calorimetric analysis of lectin-sugar interaction.
abstract: Isothermal titration calorimetry (ITC) is a powerful tool for analyzing lectin-glycan interactions because it can measure the binding affinity and thermodynamic properties such as ∆H and ΔS in a single experiment without any chemical modification or immobilization. Here we describe a method for preparing glycan and lectin solution to minimize the buffer mismatch, setting parameters, and performing experiments.
authors.affiliation: ERATO, Ito Glycotrilogy Project, Japan Science and Technology Agency (JST), 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Takeda
authors.forename: Ichiro
authors.initials: I
authors.lastname: Matsuo
mesh_terms: D002021:Buffers; D002151:Calorimetry / Q000295:instrumentation / Q000379:methods*; D007700:Kinetics; D037102:Lectins / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D011485:Protein Binding; D013816:Thermodynamics
chemical_list: D002021:Buffers; D037102:Lectins; D011134:Polysaccharides