title: Solid-phase dispersive extraction method for analysis of benzodiazepine drugs in serum and urine samples.
abstract: A simple yet highly efficient pretreatment method called solid-phase dispersive extraction (SPDE) was developed and used in combination with liquid chromatography/time-of-flight mass spectrometry (LC/TOF-MS) for the analysis of benzodiazepines (BZPs) in serum and urine samples. By using a custom-made centrifugal filter, SPDE could be performed in a closed system, thereby minimizing exposure to infectious microbes or hazardous chemicals. The limit of detection and the limit of quantification of nine BZPs were 1-10 and 5-50ng/mL, respectively. The average recoveries of BZPs from pooled serum samples spiked at 50 and 500ng/mL were 89.6-105.0% (RSD: 2.1-6.8%) and 93.6-110.4% (RSD: 2.1-4.2%), respectively, and those from urine samples were 88.7-105.5% (RSD: 2.9-6.4%) and 91.5-101.1% (RSD: 3.6-5.5%), respectively. SPDE-LC/TOF-MS has potential application in forensic science and emergency medicine.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan. Electronic address: k-saitok@hoshi.ac.jp.
authors.forename: Koichi
authors.initials: K
authors.lastname: Saito
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
authors.forename: Yuu
authors.initials: Y
authors.lastname: Kikuchi
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
authors.forename: Rieko
authors.initials: R
authors.lastname: Saito
mesh_terms: D001569:Benzodiazepines / Q000097:blood* / Q000627:therapeutic use / Q000652:urine*; D002138:Calibration; D002853:Chromatography, Liquid; D016903:Drug Monitoring / Q000379:methods* / Q000592:standards; D044707:Forensic Sciences / Q000379:methods* / Q000592:standards; D006801:Humans; D057230:Limit of Detection; D013058:Mass Spectrometry; D012015:Reference Standards; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods* / Q000592:standards; D015813:Substance Abuse Detection / Q000379:methods* / Q000592:standards; D019966:Substance-Related Disorders / Q000097:blood / Q000175:diagnosis* / Q000652:urine
chemical_list: D001569:Benzodiazepines
keywords: Abused prescription drug; Benzodiazepine; Forensic science; LC/TOF-MS; Solid-phase dispersive extraction