title: Toxicity of naturally occurring Bio-fly and chitosan compounds to control the Mediterranean fruit fly Ceratitis capitata (Wiedemann).
abstract: The efficacy of five compounds of a biopolymer chitosan and Bio-fly (Beauveria bassiana fungus) as biopesticide was evaluated on Ceratitis capitata under laboratory conditions. The inhibitory effects on acetylcholinesterase (AChE) and adenosinetriphosphatase (ATPase) as biochemical indicators were also determined in vivo. The results indicated that B. bassiana based Bio-fly exhibited significant toxicity against C. capitata (LC50 = 3008 and 3126 mg/L after 48 h in females and males, respectively) followed by the derivatives of chitosan, N-(4-propylbenzyl)chitosan and N-(2-nitrobenzyl)chitosan. Bio-fly displayed remarkable inhibition of AChE activity (IC50 = 2220 mg/L) while N-(2-chloro,6-flourobenzyl)chitosan, N-(4-propylbenzyl)chitosan and N-(3,4-methylenedioxybenzyl) chitosan had no significant difference in inhibitory action. In adult males, N-(2-nitrobenzyl)chitosan exhibited the highest inhibitory action (IC50 = 6569 mg/L). In addition, the toxic effects of the tested compounds on the activity of ATPase indicated that highly significant inhibition was found with N-(4-propylbenzyl)chitosan with an IC50 of 8194 and 8035 mg/L, in females and males, respectively.
authors.affiliation: a Department of Plant Protection , Faculty of Agriculture, Damanhour University , Damanhour , Egypt.
authors.forename: E I
authors.initials: EI
authors.lastname: Rabea
authors.forename: H M
authors.initials: HM
authors.lastname: Nasr
authors.forename: M E I
authors.initials: ME
authors.lastname: Badawy
authors.forename: I R
authors.initials: IR
authors.lastname: El-Gendy
mesh_terms: D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000818:Animals; D052982:Beauveria; D033622:Ceratitis capitata; D048271:Chitosan / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000737:chemistry; D005260:Female; D007306:Insecticides; D008297:Male; D010572:Pest Control, Biological
chemical_list: D002800:Cholinesterase Inhibitors; D007306:Insecticides; D048271:Chitosan; D000251:Adenosine Triphosphatases
keywords: AChE; ATPase; Bio-fly; Ceratitis capitata; Mediterranean fruit fly; chitosan