title: Development and characterization of microsatellite loci for Tabebuia cassinoides (Bignoniaceae).
abstract: Tabebuia cassinoides (Lam.) DC., popularly known as caxeta, is a tree species that belongs to the plant family Bignoniaceae. This species is endemic to the Brazilian Atlantic Forest and is widely exploited commercially. To date, little is known about its genetic structure, preventing the establishment of adequate management plans for this taxon. The objective of this study was to construct a microsatellite-enriched genomic library for T. cassinoides to select polymorphic loci, and standardize polymerase chain reaction amplification conditions. Of the 15 loci examined, 5 were polymorphic. The number of alleles per locus ranged from 2 to 8, with a mean of 4.4. The microsatellite loci described here represent the basis for detailed population genetic studies of this species, which will greatly contribute for the development of better conservation strategies for this taxon.
authors.affiliation: Laboratório de Sistemática Vegetal, Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brasil vaniapretti@gmail.com.
authors.forename: V Q
authors.initials: VQ
authors.lastname: Pretti
authors.affiliation: Laboratório de Genética e Evolução de Abelhas, Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: E
authors.initials: E
authors.lastname: Françoso
authors.affiliation: Laboratório de Análise Genética e Molecular, Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, SP, Brasil.
authors.forename: D A
authors.initials: DA
authors.lastname: Sforça
authors.affiliation: Instituto de Botânica, São Paulo, SP, Brasil.
authors.forename: F
authors.initials: F
authors.lastname: Pinheiro
authors.affiliation: Laboratório de Biologia Evolutiva e Conservação de Vertebrados, Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: D
authors.initials: D
authors.lastname: Meyer
authors.affiliation: Laboratório de Sistemática Vegetal, Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: L G
authors.initials: LG
authors.lastname: Lohmann
mesh_terms: D000483:Alleles; D018744:DNA, Plant; D056426:Genetic Loci; D005838:Genotype; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D029663:Tabebuia / Q000235:genetics*
chemical_list: D018744:DNA, Plant