title: De novo comparative transcriptome analysis of Acremonium chrysogenum: high-yield and wild-type strains of cephalosporin C producer.
abstract: β-lactam antibiotics are widely used in clinic. Filamentous fungus Acremonium chrysogenum is an important industrial fungus for the production of CPC, one of the major precursors of β-lactam antibiotics. Although its fermentation yield has been bred significantly over the past decades, little is known regarding molecular changes between the industrial strain and the wild type strain. This limits the possibility to improve CPC production further by molecular breeding. Comparative transcriptome is a powerful tool to understand the molecular mechanisms of CPC industrial high yield producer compared to wild type. A total of 57 million clean sequencing reads with an average length of 100 bp were generated from Illumina sequencing platform. 22,878 sequences were assembled. Among the assembled unigenes, 9502 were annotated and 1989 annotated sequences were assigned to 121 pathways by searching against the Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) database. Furthermore, we compared the transcriptome differences between a high-yield and a wild-type strain during fermentation. A total of 4329 unigenes with significantly different transcription level were identified, among which 1737 were up-regulated and 2592 were down-regulated. 24 pathways were subsequently determined which involve glycerolipid metabolism, galactose metabolism, and pyrimidine metabolism. We also examined the transcription levels of 18 identified genes, including 11 up-regulated genes and 7 down-regulated genes using reverse transcription quantitative -PCR (RT-qPCR). The results of RT-qPCR were consistent with the Illumina sequencing. In this study, the Illumina sequencing provides the most comprehensive sequences for gene expression profile of Acremonium chrysogenum and allows de novo transcriptome assembly while lacking genome information. Comparative analysis of RNA-seq data reveals the complexity of the transcriptome in the fermentation of different yield strains. This is an important public information platform which could be used to accelerate the research to improve CPC production in Acremonium chrysogenum.
authors.affiliation: China State Institute of Pharmaceutical Industry, Zhangjiang Institute, Shanghai, China; Shanghai Institute of Pharmaceutical Industry, Shanghai, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: China State Institute of Pharmaceutical Industry, Zhangjiang Institute, Shanghai, China; Shanghai Institute of Pharmaceutical Industry, Shanghai, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Xie
authors.affiliation: China State Institute of Pharmaceutical Industry, Zhangjiang Institute, Shanghai, China; Shanghai Institute of Pharmaceutical Industry, Shanghai, China.
authors.forename: Guihua
authors.initials: G
authors.lastname: Gong
authors.affiliation: China State Institute of Pharmaceutical Industry, Zhangjiang Institute, Shanghai, China; Shanghai Institute of Pharmaceutical Industry, Shanghai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: China State Institute of Pharmaceutical Industry, Zhangjiang Institute, Shanghai, China.
authors.forename: Baoquan
authors.initials: B
authors.lastname: Zhu
authors.affiliation: China State Institute of Pharmaceutical Industry, Zhangjiang Institute, Shanghai, China; Shanghai Institute of Pharmaceutical Industry, Shanghai, China.
authors.forename: Youjia
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000164:Acremonium / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D057005:Bioengineering / Q000379:methods; D019149:Bioreactors / Q000382:microbiology*; D002511:Cephalosporins / Q000096:biosynthesis*; D020869:Gene Expression Profiling / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D002511:Cephalosporins; C025163:cephalosporin C