title: Cisplatin-induced testicular toxicity in rats: the protective effect of arjunolic acid.
abstract: In the present study, the effect of arjunolic acid on testicular damage induced by intraperitoneal injection of rats with 7 mg/kg cisplatin was studied. Cisplatin induced a significant reduction in testicular weights, plasma testosterone, and testicular reduced glutathione levels in addition to a significant elevation of testicular malondialdehyde levels and testicular gene expressions of inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), and p38 mitogen-activated protein kinase (MAPK) when compared with the control group (p < 0.05). Lower tubular diameters and depletion of germ cells and irregular small seminiferous tubules with Sertoli cells only were observed in the cisplatin group. Arjunolic acid administration significantly corrected the changes in both biochemical and histopathological parameters. Arjunolic acid plays a significant protective role against cisplatin-induced testicular injury by attenuating oxidative stress parameters along with downregulation of iNOS, TNF-α, and p38-MAPK testicular expressions.
authors.affiliation: Department of Biochemistry, Mansoura University, Mansoura, Egypt. emyosh@yahoo.com.
authors.forename: Iman O
authors.initials: IO
authors.lastname: Sherif
authors.forename: Azza
authors.initials: A
authors.lastname: Abdel-Aziz
authors.forename: Osama M
authors.initials: OM
authors.lastname: Sarhan
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002945:Cisplatin / Q000633:toxicity*; D005978:Glutathione / Q000737:chemistry; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000737:chemistry; D052247:Nitric Oxide Synthase Type II / Q000235:genetics; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000166:cytology; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000097:blood; D014315:Triterpenes / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics
chemical_list: D000970:Antineoplastic Agents; D014315:Triterpenes; D014409:Tumor Necrosis Factor-alpha; D013739:Testosterone; C061640:arjunolic acid; D008315:Malondialdehyde; D052247:Nitric Oxide Synthase Type II; D048051:p38 Mitogen-Activated Protein Kinases; D005978:Glutathione; D002945:Cisplatin
keywords: Arjunolic Acid; Cisplatin; Oxidative Stress; Rat; Testis; Toxicity