title: The minor wall-networks between monolignols and interlinked-phenolics predominantly affect biomass enzymatic digestibility in Miscanthus.
abstract: Plant lignin is one of the major wall components that greatly contribute to biomass recalcitrance for biofuel production. In this study, total 79 representative Miscanthus germplasms were determined with wide biomass digestibility and diverse monolignol composition. Integrative analyses indicated that three major monolignols (S, G, H) and S/G ratio could account for lignin negative influence on biomass digestibility upon NaOH and H2SO4 pretreatments. Notably, the biomass enzymatic digestions were predominately affected by the non-KOH-extractable lignin and interlinked-phenolics, other than the KOH-extractable ones that cover 80% of total lignin. Furthermore, a positive correlation was found between the monolignols and phenolics at p<0.05 level in the non-KOH-extractable only, suggesting their tight association to form the minor wall-networks against cellulases accessibility. The results indicated that the non-KOH-extractable lignin-complex should be the target either for cost-effective biomass pretreatments or for relatively simply genetic modification of plant cell walls in Miscanthus.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Biomass and Bioenergy Research Centre, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Zhengru
authors.initials: Z
authors.lastname: Li
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Biomass and Bioenergy Research Centre, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Chunqiao
authors.initials: C
authors.lastname: Zhao
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Biomass and Bioenergy Research Centre, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zha
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Biomass and Bioenergy Research Centre, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Can
authors.initials: C
authors.lastname: Wan
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Biomass and Bioenergy Research Centre, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Si
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Biomass and Bioenergy Research Centre, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Biomass and Bioenergy Research Centre, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Biomass and Bioenergy Research Centre, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Fengcheng
authors.initials: F
authors.lastname: Li
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Biomass and Bioenergy Research Centre, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yu
authors.affiliation: Department of Biotechnology, Hunan Agricultural University, Changsha, China.
authors.forename: Zili
authors.initials: Z
authors.lastname: Yi
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Biomass and Bioenergy Research Centre, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China; Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaiyin Normal University, Huaian, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xu
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Biomass and Bioenergy Research Centre, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China; National Key Laboratory of Crop Genetic Improvement, Biomass and Bioenergy Research Centre, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Liangcai
authors.initials: L
authors.lastname: Peng
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Biomass and Bioenergy Research Centre, College of Science, Huazhong Agricultural University, Wuhan, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
mesh_terms: D018533:Biomass; D044602:Cellulases / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D006109:Poaceae / Q000235:genetics / Q000378:metabolism*
chemical_list: D010636:Phenols; D008031:Lignin; D044602:Cellulases