title: The excreted polysaccharide of Pleurotus eryngii inhibits the foam-cell formation via down-regulation of CD36.
abstract: Previous study has verified the polysaccharide from the fruiting body of Pleurotus eryngii (PEPE) is capable of decreasing the lipid content in both of cell-line and mouse model. However, little is known about underlying mechanisms and whether this bioactive polysaccharide exists in submerged culture. Here, we verified the excreted polysaccharides EP and EP-1 from submersion culture of P. eryngii have the remarkable inhibitory effects on lipid accumulation in macrophage-derived foam cells. Structure analysis indicates EP-1 consists of D-types of glucose, galactose and mannose with the main β(1 → 3)-glucan glycosidic linkage branched at O-6 by α-D-glucose while EP digested by β-1,3-glucanase fails to decrease the lipid accumulation, suggesting that the special structure is essential for its function. Expression analysis suggests that EP is able to cause the down-regulation of the scavenger receptor-CD36 on both transcription and protein levels. Most importantly, EP can be obtained by fermentation in a mass-production.
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology, College of Life Sciences, Nanjing Normal University, No. 1 Wen Yuan Rd, Qi Xia Qu, Nanjing, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology, College of Life Sciences, Nanjing Normal University, No. 1 Wen Yuan Rd, Qi Xia Qu, Nanjing, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Yong
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology, College of Life Sciences, Nanjing Normal University, No. 1 Wen Yuan Rd, Qi Xia Qu, Nanjing, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Xia
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology, College of Life Sciences, Nanjing Normal University, No. 1 Wen Yuan Rd, Qi Xia Qu, Nanjing, China.
authors.forename: Zeliang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology, College of Life Sciences, Nanjing Normal University, No. 1 Wen Yuan Rd, Qi Xia Qu, Nanjing, China.
authors.forename: Youxing
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology, College of Life Sciences, Nanjing Normal University, No. 1 Wen Yuan Rd, Qi Xia Qu, Nanjing, China.
authors.forename: Shizhu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology, College of Life Sciences, Nanjing Normal University, No. 1 Wen Yuan Rd, Qi Xia Qu, Nanjing, China. Electronic address: linglu@njnu.edu.cn.
authors.forename: Ling
authors.initials: L
authors.lastname: Lu
mesh_terms: D000818:Animals; D018955:CD36 Antigens / Q000378:metabolism*; D002236:Carbohydrate Conformation; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects; D005487:Foam Cells / Q000187:drug effects* / Q000378:metabolism; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D050356:Lipid Metabolism / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000032:analysis; D051379:Mice; D008970:Molecular Weight; D020076:Pleurotus / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D018955:CD36 Antigens; D062610:Fungal Polysaccharides; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D005947:Glucose; D008358:Mannose; D005690:Galactose
keywords: Excreted polysaccharide; Lipid accumulation; Pleurotus eryngii; Scavenger receptor; Submerged culture