title: Protective effect of naringenin against gentamicin-induced nephrotoxicity in rats.
abstract: The protective effect of naringenin, a flavonoid compound isolated from citrus fruits, was investigated against nephrotoxicity induced by gentamicin (80mgkg(-1)/day, i.p., for eight days) in rats. Naringenin treatment (50mgkg(-1)/day, p.o.) was administered for eight days, starting on the same day of gentamicin administration. Gentamicin caused significant elevations of serum creatinine, and kidney tissue levels of malondialdehyde, nitric oxide, and interleukin-8, and a significant decrease in renal glutathione peroxidase activity. Naringenin treatment significantly ameliorated the changes in the measured biochemical parameters resulted from gentamicin administration. Also, naringenin markedly attenuated the histopathological renal tissue injury observed with gentamicin. Immunohistochemical examinations showed that naringenin significantly reduced the gentamicin-induced expression of kidney injury molecule-1, vascular endothelial growth factor, inducible nitric oxide synthase, and caspase-9, and increased survivin expression in the kidney tissue. It was concluded that naringenin, through its antioxidant and anti-inflammatory effects, may represent a therapeutic option to protect against gentamicin nephrotoxicity.
authors.affiliation: Department of Biomedical Sciences, Pharmacology Division, College of Medicine, King Faisal University, Al-Ahsa, Saudi Arabia. Electronic address: amrfouad65@yahoo.com.
authors.forename: Amr A
authors.initials: AA
authors.lastname: Fouad
authors.affiliation: Department of Pediatrics, College of Medicine, King Faisal University, Al-Ahsa, Saudi Arabia.
authors.forename: Waleed H
authors.initials: WH
authors.lastname: Albuali
authors.affiliation: Department of Internal Medicine, Nephrology Division, College of Medicine, King Faisal University, Al-Ahsa, Saudi Arabia.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Zahran
authors.affiliation: Department of Pathology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Wafaey
authors.initials: W
authors.lastname: Gomaa
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D004336:Drug Antagonism; D044950:Flavanones / Q000008:administration & dosage* / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D005839:Gentamicins / Q000009:adverse effects*; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D007677:Kidney Function Tests; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000008:administration & dosage* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D051437:Renal Insufficiency / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology
chemical_list: D000900:Anti-Bacterial Agents; D044950:Flavanones; D005839:Gentamicins; D020011:Protective Agents; C005273:naringenin
keywords: Acute kidney injury; Gentamicin; Naringenin; Rats