title: An electrochemical immunosensor based on interdigitated array microelectrode for the detection of chlorpyrifos.
abstract: An electrochemical immunosensor based on interdigitated array microelectrodes (IDAMs) was developed for sensitive, specific and rapid detection of chlorpyrifos. Anti-chlorpyrifos monoclonal antibodies were orientedly immobilized onto the gold microelectrode surface through protein A. Chlorpyrifos were then captured by the immobilized antibody, resulting in an impedance change in the IDAMs surface. Electrochemical impedance spectroscopy was used in conjunction with the fabricated sensor to detect chlorpyrifos. Under optimum conditions, the impedance value change of chlorpyrifos was proportional to its concentrations in the range of 10(0)-10(5) ng/mL. The detection limit was found to be 0.014 ng/mL for chlorpyrifos. The proposed chlorpyrifos immunosensor could be used as a screening method in pesticide determination for the analysis of environmental, agricultural and pharmaceutical samples due to its rapidity, sensitivity and low cost.
authors.affiliation: School of Agricultural and Food Engineering, Shandong University of Technology, No. 12, Zhangzhou Road, Zibo, 255049, Shandong, People's Republic of China.
authors.forename: Yaoyao
authors.initials: Y
authors.lastname: Cao
authors.forename: Xia
authors.initials: X
authors.lastname: Sun
authors.forename: Yemin
authors.initials: Y
authors.lastname: Guo
authors.forename: Wenping
authors.initials: W
authors.lastname: Zhao
authors.forename: Xiangyou
authors.initials: X
authors.lastname: Wang
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation; D004390:Chlorpyrifos / Q000032:analysis* / Q000276:immunology; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D007306:Insecticides / Q000032:analysis / Q000276:immunology; D046228:Microarray Analysis / Q000295:instrumentation*; D008839:Microelectrodes; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D007306:Insecticides; D004390:Chlorpyrifos