title: [Treatment of autotransplantation of NT-3 gene modified OECs and NSCs complex adhering to scaffold on spontaneous cerebral hemorrhage].
abstract: OBJECTIVE
  To explore the effects of autotransplantation of NT-3 gene modified olfactory ensheathing cell (OEC) and neural stem cell (NSC) complex adhering to collagen protein-heparin sulfate scaffold on motor function of rats with cerebral hemorrhage.
  
  
  METHODS
  The cerebral hemorrhage model was established with caudate nucleus bleeding in Wistar rats. The animals were randomly divided into 4 groups: A (transplantation of NT-3 modified OEC-NSC complex adhering to scaffold), B (transplantation of NT-3 modified OEC-NSC complex), C (transplantation of scaffold) and D (blank control group). The motor function of hind limbs was assessed at Day 1, 3, 7, 14 and 30 post-transplantation respectively. The survival, distribution and differentiation of transplanted cells were tested by immunohistochemistry and fluorescent staining.
  
  
  RESULTS
  The neurological functional score of group A (2.12 ± 0.12, 1.50 ± 0.11, 0.52 ± 0.08) or B (2.10 ± 0.16, 1.79 ± 0.09, 0.91 ± 0.10) was obviously inferior to group C/D at Days 7, 14 and 30. No significant difference existed between groups C and D (P > 0.05) . The scores of A were markedly lower than those of B at Days 14 and 30 (P < 0.05, P < 0.01) . The numbers of surviving NSCs and cells migrating to focal area after transplantation was much more in group A than those in other groups.
  
  
  CONCLUSIONS
  Autotransplantation of NT-3 gene modified OEC-NSC complex adhering to collagen protein-heparin sulfate scaffold may markedly ameliorate the motor function of cerebral hemorrhagic in rats. And the transplanted NSCs have better capacities of survival, migration and differentiation.
authors.affiliation: Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China.
authors.forename: Chunjuan
authors.initials: C
authors.lastname: Wang
authors.forename: Jinshan
authors.initials: J
authors.lastname: Yang
authors.forename: Huawei
authors.initials: H
authors.lastname: Lin
authors.forename: Peicai
authors.initials: P
authors.lastname: Fu
authors.affiliation: Department of Neurology, Affiliated Provincial Hospital, Shandong University, Jinan 250021, China. Email: guoshougang@163.com.
authors.forename: Shougang
authors.initials: S
authors.lastname: Guo
authors.forename: Ronghua
authors.initials: R
authors.lastname: Tang
mesh_terms: D000818:Animals; D002543:Cerebral Hemorrhage / Q000601:surgery*; D004195:Disease Models, Animal; D008297:Male; D058953:Neural Stem Cells / Q000637:transplantation*; D020933:Neurotrophin 3 / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D033581:Stem Cell Transplantation; D014182:Transplantation, Autologous
chemical_list: D020933:Neurotrophin 3