title: Ligularia fischeri extract protects against oxidative-stress-induced neurotoxicity in mice and PC12 cells.
abstract: Alzheimer's disease (AD) is pathologically characterized by the presence of amyloid plaques in brain and the overproduction of amyloid beta (Aβ), leading to learning and memory impairment and intense oxidative stress. In this study, the protective effect of Ligularia fischeri extract was investigated using PC12 cells. L. fischeri extract attenuated hydrogen-peroxide-induced DNA fragmentation in cells. In vivo behavioral tests were performed to examine the effects of the extract on amyloid-β peptide1-42-induced impairment of learning and memory in mice. A diet containing the extract increased alternation behaviors in the Y-maze test and step-through latency of passive avoidance task. Moreover, we found that consumption of the extract decreased lipid peroxidation in a biochemical study of brain tissue in mice. High-performance liquid chromatography was used to identify the active compounds in the extract. These results suggest that L. fischeri extract could be protective against Aβ-induced neurotoxicity, possibly due to the antioxidative capacity of its constituent, 3-O-caffeoylquinic acid.
authors.affiliation: 1 Functional Food Research Center, Korea University , Seoul, Korea.
authors.forename: Soo Jung
authors.initials: SJ
authors.lastname: Choi
authors.forename: Jae Kyeom
authors.initials: JK
authors.lastname: Kim
authors.forename: Soo Hwan
authors.initials: SH
authors.lastname: Suh
authors.forename: Cho Rong
authors.initials: CR
authors.lastname: Kim
authors.forename: Hye Kyung
authors.initials: HK
authors.lastname: Kim
authors.forename: Chang-Ju
authors.initials: CJ
authors.lastname: Kim
authors.forename: Gwi Gun
authors.initials: GG
authors.lastname: Park
authors.forename: Cheung-Seog
authors.initials: CS
authors.lastname: Park
authors.forename: Dong-Hoon
authors.initials: DH
authors.lastname: Shin
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism / Q000517:prevention & control; D016229:Amyloid beta-Peptides / Q000378:metabolism* / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D019659:Asteraceae / Q000737:chemistry*; D001362:Avoidance Learning / Q000187:drug effects; D001921:Brain / Q000187:drug effects*; D002726:Chlorogenic Acid / Q000494:pharmacology / Q000627:therapeutic use; D003072:Cognition Disorders / Q000139:chemically induced / Q000235:genetics / Q000517:prevention & control; D053938:DNA Fragmentation / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000633:toxicity; D007859:Learning Disabilities / Q000139:chemically induced / Q000517:prevention & control; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008568:Memory / Q000187:drug effects; D008569:Memory Disorders / Q000139:chemically induced / Q000517:prevention & control; D008813:Mice, Inbred ICR; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use; D020258:Neurotoxicity Syndromes / Q000235:genetics / Q000517:prevention & control*; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats
chemical_list: D016229:Amyloid beta-Peptides; D000975:Antioxidants; D018696:Neuroprotective Agents; D010936:Plant Extracts; D002726:Chlorogenic Acid; D006861:Hydrogen Peroxide
keywords: 3-O-caffeoylquinic acid; Ligularia fischeri; amyloid beta peptide; oxidative stress