title: A new structure determination method of lectins using a selenium-containing sugar ligand.
abstract: Phase determination is essential for solving X-ray crystal structures of proteins and their complexes. Conventional phase-determination methods using heavy atoms (Pt, Au, Hg, etc.) or the selenium (Se) atom are routinely utilized in structure determination of protein crystals. Here, we describe an alternative phase-determination method for proteins such as lectins in which a Se-containing glycan is used as a ligand. In this technique, the Se atoms are simply introduced into the protein crystal as a complex, and the phase of the protein can be determined using anomalous signals from the Se-containing sugar.
authors.affiliation: Photon Factory, Structural Biology Research Center, IMSS, High Energy Accelerator Research Organization (KEK), Tsukuba, Ibaraki, 305-0801, Japan.
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Makyio
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Kato
mesh_terms: D018360:Crystallography, X-Ray / Q000379:methods*; D037161:Galectins / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D011134:Polysaccharides / Q000737:chemistry; D017433:Protein Structure, Secondary; D012643:Selenium
chemical_list: D037161:Galectins; C105032:LGALS9 protein, human; D008024:Ligands; D011134:Polysaccharides; D012643:Selenium