title: Oxidized multiwalled carbon nanotubes as antigen delivery system to promote superior CD8(+) T cell response and protection against cancer.
abstract: Properties like high interfacial area with cellular membranes, unique ability to incorporate multiple functionalization, as well as compatibility and transport in biological fluids make carbon nanotubes (CNTs) useful for a variety of therapeutic and drug-delivery applications. Here we used a totally synthetic hybrid supramolecule as an anticancer vaccine formulation. This complex structure comprises CNTs as delivery system for the Cancer Testis Antigen named NY-ESO-1, allied to a synthetic Toll-Like Receptor agonist. The CNT constructs were rapidly internalized into dendritic cells, both in vitro and in vivo, and served as an intracellular antigen depot. This property favored the induction of strong CD4(+) T as well as CD8(+) T cell-mediated immune responses against the NY-ESO-1. Importantly, the vaccination significantly delayed the tumor development and prolonged the mice survival, highlighting the potential application of CNTs as a vaccine delivery system to provide superior immunogenicity and strong protection against cancer.
authors.affiliation: Instituto de Pesquisa René Rachou , Fundação Oswaldo Cruz, 30190-002 Belo Horizonte, MG Brazil.
authors.forename: Paula Cristina Batista
authors.initials: PC
authors.lastname: de Faria
authors.forename: Luara Isabela
authors.initials: LI
authors.lastname: dos Santos
authors.forename: João Paulo
authors.initials: JP
authors.lastname: Coelho
authors.forename: Henrique Bücker
authors.initials: HB
authors.lastname: Ribeiro
authors.forename: Marcos Assunção
authors.initials: MA
authors.lastname: Pimenta
authors.forename: Luiz Orlando
authors.initials: LO
authors.lastname: Ladeira
authors.forename: Dawidson Assis
authors.initials: DA
authors.lastname: Gomes
authors.forename: Clascídia Aparecida
authors.initials: CA
authors.lastname: Furtado
authors.forename: Ricardo Tostes
authors.initials: RT
authors.lastname: Gazzinelli
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000737:chemistry; D000941:Antigens / Q000737:chemistry; D000951:Antigens, Neoplasm / Q000737:chemistry; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology*; D002138:Calibration; D019496:Cancer Vaccines / Q000737:chemistry*; D049109:Cell Proliferation; D018899:CpG Islands; D003713:Dendritic Cells / Q000187:drug effects; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008214:Lymphocytes / Q000166:cytology; D008565:Membrane Proteins / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009369:Neoplasms / Q000378:metabolism / Q000517:prevention & control* / Q000628:therapy*; D010100:Oxygen / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D013059:Spectrum Analysis, Raman
chemical_list: D016588:Anticarcinogenic Agents; D000941:Antigens; D000951:Antigens, Neoplasm; C104948:CTAG1B protein, human; D019496:Cancer Vaccines; D008565:Membrane Proteins; D037742:Nanotubes, Carbon; D010455:Peptides; D010100:Oxygen
keywords: CD8+ activation; NY-ESO-1; Nanovaccine; cancer; carbon nanotubes