title: Inkjet-printed bioassays for direct reading with a multimode DVD/Blu-Ray optical drive.
abstract: Compact disc-based bioassays have been developed as novel point-of-care (POC) tools for various applications in chemical analysis and biomedical diagnosis. For the fabrication of assay discs, the surface patterning and sample introduction have been restricted to manual delivery that is unfavorable for on-demand high throughput medical screening. Herein, we have adapted a conventional inkjet printer to prepare bioassays on regular DVD-Rs and accomplished quantitative analysis with a multimode DVD/Blu-Ray optical drive in conjunction with free disc diagnostic software. The feasibility and accuracy of this method have been demonstrated by the quantitative analysis of inkjet-printed biotin-streptavidin binding assays on DVD, which serves as a trial system for other complex, medically relevant sandwich-format or competitive immunoassays.
authors.affiliation: Key Laboratory of Advanced Transducers and Intelligent Control Systems (Ministry of Education and Shanxi Province), College of Physics and Optoelectronics, Taiyuan University of Technology , Taiyuan, Shanxi 030024, P. R. China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Li
authors.forename: Maolin
authors.initials: M
authors.lastname: Shi
authors.forename: Caie
authors.initials: C
authors.lastname: Cui
authors.forename: Hua-Zhong
authors.initials: HZ
authors.lastname: Yu
mesh_terms: D001681:Biological Assay; D001710:Biotin / Q000737:chemistry / Q000378:metabolism*; D016250:Compact Disks; D019095:Point-of-Care Systems; D011327:Printing; D011485:Protein Binding; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001710:Biotin; D019809:Streptavidin