title: Capillary-based lectin affinity electrophoresis for interaction analysis between lectins and glycans.
abstract: Capillary affinity electrophoresis (CAE) is a powerful technique for glycan analysis, and one of the analytical approaches for analyzing the interaction between lectins and glycans. The method is based on the high-resolution separation of fluorescently labeled glycans by capillary electrophoresis (CE) with laser-induced fluorescence detection (LIF) in the presence of lectins (or glycan binding proteins). CAE allows simultaneous determination of glycan structures in a complex mixture of glycans. In addition, we can calculate the binding kinetics on a specific glycan in the complex mixture of glycans with a lectin. Here, we show detailed procedures for capillary affinity electrophoresis of fluorescently labeled glycans with lectins using CE-LIF apparatus. Its application to screening a sialic acid binding protein in plant barks is also shown.
authors.affiliation: Department of Pharmaceutical Sciences, School of pharmacy, Kinki University, Kowakae3-4-1, Higashi-osaka, 577-8502, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Kinoshita
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Kakehi
mesh_terms: D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D007834:Lasers; D037102:Lectins / Q000378:metabolism*; D008892:Milk / Q000737:chemistry; D009844:Oligosaccharides / Q000378:metabolism; D009961:Orosomucoid / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D005456:Fluorescent Dyes; D037102:Lectins; D009844:Oligosaccharides; D009961:Orosomucoid; D011134:Polysaccharides