title: Evaluation of in vitro cytotoxicity and genotoxicity of copper-zinc alloy nanoparticles in human lung epithelial cells.
abstract: In the present study, in vitro cytotoxic and genotoxic effect of copper-zinc alloy nanoparticles (Cu-Zn ANPs) on human lung epithelial cells (BEAS-2B) were investigated. XTT test and clonogenic assay were used to determine cytotoxic effects. Cell death mode and intracellular reactive oxygen species formations were analyzed using M30, M65 and ROS Elisa assays. Genotoxic effects were evaluated using micronucleus, comet and γ-H2AX foci assays. Cu-Zn ANPs were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and zeta potential measurements. Characterization of Cu-Zn ANPs showed an average size of 200nm and zeta potential of -22mV. TEM analyses further revealed the intracellular localization of Cu-Zn ANPs in cytoplasm within 24h. Analysis of micronucleus, comet and γ-H2AX foci counts showed that exposure to Cu-Zn ANPs significantly induced chromosomal damage as well as single and double stranded DNA damage in BEAS-2B cells. Our results further indicated that exposure to Cu-Zn ANPs significantly induced intracellular ROS formation. Evaluation of M30:M65 ratios suggested that cell death was predominantly due to necrosis.
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science and Art, Nevşehir University, 50300 Nevşehir, Turkey.
authors.forename: Umit
authors.initials: U
authors.lastname: Kumbıçak
authors.affiliation: Cell Culture and Genetic Toxicology Laboratory, Department of Biology, Faculty of Sciences and Arts, Uludağ University, 16059 Nilüfer, Bursa, Turkey. Electronic address: tcavas@uludag.edu.tr.
authors.forename: Tolga
authors.initials: T
authors.lastname: Cavaş
authors.affiliation: Cell Culture and Genetic Toxicology Laboratory, Department of Biology, Faculty of Sciences and Arts, Uludağ University, 16059 Nilüfer, Bursa, Turkey.
authors.forename: Nilüfer
authors.initials: N
authors.lastname: Cinkılıç
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science and Art, Nevşehir University, 50300 Nevşehir, Turkey.
authors.forename: Zübeyde
authors.initials: Z
authors.lastname: Kumbıçak
authors.affiliation: Cell Culture and Genetic Toxicology Laboratory, Department of Biology, Faculty of Sciences and Arts, Uludağ University, 16059 Nilüfer, Bursa, Turkey.
authors.forename: Ozgür
authors.initials: O
authors.lastname: Vatan
authors.affiliation: Cell Culture and Genetic Toxicology Laboratory, Department of Biology, Faculty of Sciences and Arts, Uludağ University, 16059 Nilüfer, Bursa, Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Yılmaz
mesh_terms: D017209:Apoptosis; D015197:Carcinogenicity Tests; D003300:Copper / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000633:toxicity*; D046529:Microscopy, Electron, Transmission; D009152:Mutagenicity Tests; D017382:Reactive Oxygen Species / Q000378:metabolism; D015032:Zinc / Q000737:chemistry*
chemical_list: D017382:Reactive Oxygen Species; D003300:Copper; D015032:Zinc
keywords: BEAS-2B cells; Copper–zinc alloy nanoparticles; Cytotoxicity; Genotoxicity; Nanotoxicology