title: Aldo-keto reductase family 1 member B8 is secreted via non-classical pathway.
abstract: Mouse aldo-keto reductase family 1 member B8 (AKR1B8) has the highest similarity to human aldo-keto reductase family 1 member B10 (AKR1B10), a secretory protein through lysosomes-mediated non-classical secretory pathway. To identify whether AKR1B8 is secreted through the same pathway, we carried out this study. Self-developed sandwich ELISA and western blot were used to detect AKR1B8 in cells and culture medium of CT-26 murine colon carcinoma cells. AKR1B8 releases in an independent manner to Brefeldin A, an inhibitor of ER-to-Golgi classical secretion pathway. Several factors, which are involved in the non-classical secretion pathway, such as temperature, ATP and calcium ion, regulated AKR1B8 secretion from mouse colorectal cancer cells CT-26. Lysosomotropic NH4Cl increased AKR1B8 secretion, and AKR1B8 was located in isolated lysosomes. Therefore, AKR1B8 is a new secretory protein through the lysosomes-mediated non-classical pathway.
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of University of South China Hengyang 421001, Hunan, China ; Hunan Engineering Laboratory of Medical Molecular Diagnosis Technology, Institute of Translational Medicine, Affliated The First People's Hospital of Chenzhou, University of South China Chenzhou 423000, Hunan, China.
authors.forename: Zhenwang
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Department of Pharmacy, The Third Affiliated Hospital of Guangzhou Medical College Guangzhou 510150, Guangdong, China.
authors.forename: Chenglai
authors.initials: C
authors.lastname: Xia
authors.affiliation: Hunan Engineering Laboratory of Medical Molecular Diagnosis Technology, Institute of Translational Medicine, Affliated The First People's Hospital of Chenzhou, University of South China Chenzhou 423000, Hunan, China.
authors.forename: Renbin
authors.initials: R
authors.lastname: Huang
authors.affiliation: Department of Medical Microbiology, Immunology & Cell Biology, Simmons Cooper Cancer Institute, Southern Illinois University School of Medicine 913 N. Rutledge Street, Springfield, IL 62702, USA ; Department of Orthopaedics, The Second Xiangya Hospital of Central South University Middle Ren-Min Road No. 139, Changsha, Hunan 410011, China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, The Second Xiangya Hospital of Central South University Middle Ren-Min Road No. 139, Changsha, Hunan 410011, China.
authors.forename: Wan-Chun
authors.initials: WC
authors.lastname: Wang
authors.affiliation: Hunan Engineering Laboratory of Medical Molecular Diagnosis Technology, Institute of Translational Medicine, Affliated The First People's Hospital of Chenzhou, University of South China Chenzhou 423000, Hunan, China.
authors.forename: Wangyuan
authors.initials: W
authors.lastname: Guo
authors.affiliation: Hunan Engineering Laboratory of Medical Molecular Diagnosis Technology, Institute of Translational Medicine, Affliated The First People's Hospital of Chenzhou, University of South China Chenzhou 423000, Hunan, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Duan
authors.affiliation: Hunan Engineering Laboratory of Medical Molecular Diagnosis Technology, Institute of Translational Medicine, Affliated The First People's Hospital of Chenzhou, University of South China Chenzhou 423000, Hunan, China.
authors.forename: Weihao
authors.initials: W
authors.lastname: Luo
authors.affiliation: Department of Medical Microbiology, Immunology & Cell Biology, Simmons Cooper Cancer Institute, Southern Illinois University School of Medicine 913 N. Rutledge Street, Springfield, IL 62702, USA.
authors.forename: Deliang
authors.initials: D
authors.lastname: Cao
authors.affiliation: Hunan Engineering Laboratory of Medical Molecular Diagnosis Technology, Institute of Translational Medicine, Affliated The First People's Hospital of Chenzhou, University of South China Chenzhou 423000, Hunan, China.
authors.forename: Di-Xian
authors.initials: DX
authors.lastname: Luo
mesh_terms: D000449:Aldehyde Reductase / Q000378:metabolism*; D000074408:Aldo-Keto Reductases; D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D008247:Lysosomes / Q000378:metabolism*; D051379:Mice; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase; C000614209:AKR7A5 protein, mouse
keywords: AKR1B8; Aldose reductase; aldose reductase family 1 member B8; non-classical pathway; secretion