title: Diallyl trisulfide induces apoptosis in Jurkat cells by the modification of cysteine residues in thioredoxin.
abstract: We reported the regulation of protein function by oxidative modification of the specific cysteine residue(s) by diallyl trisulfide (DATS). In this study, we examined if DATS modifies the cysteine residue of thioredoxin (Trx) by urea-polyacryl amide gel electrophoresis. DATS modified two specific cysteine residues in Trx and this oxidative modification of cysteine residues would be sole causative of the apoptosis induced by DATS in leukemic cells.
authors.affiliation: a Department of Chemistry and Life Science , College of Bioresource Sciences, Nihon University , Kanagawa , Japan.
authors.forename: Kazunari
authors.initials: K
authors.lastname: Watanabe
authors.forename: Takashi
authors.initials: T
authors.lastname: Hosono
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Watanabe
authors.forename: Tomomi
authors.initials: T
authors.lastname: Hosono-Fukao
authors.forename: Toyohiko
authors.initials: T
authors.lastname: Ariga
authors.forename: Taiichiro
authors.initials: T
authors.lastname: Seki
mesh_terms: D000498:Allyl Compounds / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D019169:Jurkat Cells; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D059748:Proteolysis / Q000187:drug effects; D013440:Sulfides / Q000494:pharmacology*; D013879:Thioredoxins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D000498:Allyl Compounds; D000970:Antineoplastic Agents; D013440:Sulfides; C042577:diallyl trisulfide; D013879:Thioredoxins; D011065:Poly(ADP-ribose) Polymerases; D020169:Caspases; D003545:Cysteine
keywords: apoptosis; cysteine modification; diallyl trisulfide; thioredoxin