title: Separation of cis- and trans-cypermethrin by reversed-phase high-performance liquid chromatography.
abstract: As a pyrethroid insecticide, cypermethrin (CP) possesses three chiral centers and thus consists of eight optical isomers. High-performance liquid chromatography (HPLC) methods have been established for the separation and determination of pyrethroid insecticides. In this article, we report a new reversed-phase high-performance liquid chromatography (RP-HPLC) method of separating the diastereomers of CP. Our method was performed on a C18 column with the following specifications: mobile phase, methanol/acetonitrile/water (58:18:24, v/v/v); flow rate, 1.0 mL/min; column temperature, 20°C and UV-detection wavelength, 235 nm. The results showed that the applied procedure was linear based on calibration curves with equation (y = 16.595x + 1.7892) within the CP concentration range of 5-100 mg/L. The limit of detection and limit of quantification of CP were 0.2 and 0.7 mg/L, respectively. CP or β-CP extracted from the microbial degradation system was determined, and the method was proved to be accurate and applicable. Therefore, the proposed RP-HPLC method was simple, inexpensive and can be used to study stability and determine CP residues in pharmaceutical preparations or in the environment.
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, and Laboratory of Insect Toxicology, South China Agricultural University, Guangzhou 510642, China School of Navel Orange, Gannan Normal University, Ganzhou 341000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.affiliation: College of Chemistry and Bioengineering, Yichun University, Yichun 336000, China.
authors.forename: Wanbin
authors.initials: W
authors.lastname: Xie
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, and Laboratory of Insect Toxicology, South China Agricultural University, Guangzhou 510642, China Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Chen
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, and Laboratory of Insect Toxicology, South China Agricultural University, Guangzhou 510642, China School of Navel Orange, Gannan Normal University, Ganzhou 341000, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Bioengineering, Yichun University, Yichun 336000, China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, and Laboratory of Insect Toxicology, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Dong
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, and Laboratory of Insect Toxicology, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Rashid
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, and Laboratory of Insect Toxicology, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, and Laboratory of Insect Toxicology, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Meiying
authors.initials: M
authors.lastname: Hu
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, and Laboratory of Insect Toxicology, South China Agricultural University, Guangzhou 510642, China guohuazhong@scau.edu.cn.
authors.forename: Guohua
authors.initials: G
authors.lastname: Zhong