title: The "tale" of poly(A) binding protein: the MLLE domain and PAM2-containing proteins.
abstract: The cytoplasmic poly(A) binding protein 1 (PABPC1) is an essential eukaryotic translational initiation factor first described over 40 years ago. Most studies of PABPC1 have focused on its N-terminal RRM domains, which bind the mRNA 3' poly(A) tail and 5' translation complex eIF4F via eIF4G; however, the protein also contains a C-terminal MLLE domain that binds a peptide motif, termed PAM2, found in many proteins involved in translation regulation and mRNA metabolism. Studies over the past decade have revealed additional functions of PAM2-containing proteins (PACs) in neurodegenerative diseases, circadian rhythms, innate defense, and ubiquitin-mediated protein degradation. Here, we summarize functional and structural studies of the MLLE/PAM2 interaction and discuss the diverse roles of PACs.
authors.affiliation: Department of Biochemistry and the Groupe de recherche axé sur la structure des protéines, McGill University, 3649 Promenade Sir-William-Osler, Montréal, Québec H3G 0B1, Canada.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Biochemistry and the Groupe de recherche axé sur la structure des protéines, McGill University, 3649 Promenade Sir-William-Osler, Montréal, Québec H3G 0B1, Canada.
authors.forename: Guennadi
authors.initials: G
authors.lastname: Kozlov
authors.affiliation: Department of Biochemistry and the Groupe de recherche axé sur la structure des protéines, McGill University, 3649 Promenade Sir-William-Osler, Montréal, Québec H3G 0B1, Canada. Electronic address: kalle.gehring@mcgill.ca.
authors.forename: Kalle
authors.initials: K
authors.lastname: Gehring
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D039122:Poly(A)-Binding Protein I / Q000737:chemistry* / Q000145:classification*; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*
chemical_list: D039122:Poly(A)-Binding Protein I; D012333:RNA, Messenger
keywords: Polyadenylation; RNA binding protein; Structural biology; Translational control; mRNA decay; mRNA translation