title: IL-22 promotes the migration and invasion of gastric cancer cells via IL-22R1/AKT/MMP-9 signaling.
abstract: IL-22, one important inflammatory cytokine of the IL-10 family, exerts its functions via IL-22 receptor that is composed of IL-22R1 and IL-10R2 subunits. Although IL-22 expression is reported to be elevated in many cancers, and increased IL-22 expression correlates with tumor progression and poor prognosis, little is known about the role of IL-22 in gastric cancer. In our study, we found that IL-22 stimulation promoted the migration and invasion of SGC-7901 cells. Furthermore, IL-22 increased AKT activation and MMP-9 production in a time- and dose-dependent manner, while knockdown of IL-22R1 attenuated the effect of IL-22 on gastric cancer cells. In addition, blocking of AKT activation suppressed the expression and secretion of MMP-9. Taken together, this present study suggests that IL-22 stimulation enhances the migration and invasion of gastric cancer cells by regulating IL-22R1/AKT/MMP-9 signaling axis.
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Xinxiang Medical University Xinxiang, 453000, China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Ji
authors.affiliation: Department of Pathology, Xinxiang Medical University Xinxiang, 453003, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Pathology, The First Affiliated Hospital of Xinxiang Medical University Xinxiang, 453000, China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Xinxiang Medical University Xinxiang, 453000, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Xinxiang Medical University Xinxiang, 453000, China.
authors.forename: Xiaorui
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pathology, The First Affiliated Hospital of Xinxiang Medical University Xinxiang, 453000, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Pathology, Xinxiang Medical University Xinxiang, 453003, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007378:Interleukins / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000473:pathology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D018123:Receptors, Interleukin / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D013274:Stomach Neoplasms / Q000473:pathology*; D014162:Transfection; D000097043:Interleukin-22
chemical_list: D007378:Interleukins; D020780:Matrix Metalloproteinase 9; D051057:Proto-Oncogene Proteins c-akt; D034741:RNA, Small Interfering; D018123:Receptors, Interleukin; D000097043:Interleukin-22; C415369:interleukin-22 receptor; C579270:MMP9 protein, human
keywords: IL-22; IL-22R1; MMP-9; gastric cancer; invasion; migration