title: Identification and characterization of linezolid-resistant cfr-positive Staphylococcus aureus USA300 isolates from a New York City medical center.
abstract: The cfr gene was identified in three linezolid-resistant USA300 methicillin-resistant Staphylococcus aureus (MRSA) isolates collected over a 3-day period at a New York City medical center in 2011 as part of a routine surveillance program. Each isolate possessed a plasmid containing a pSCFS3-like cfr gene environment. Transformation of the cfr-bearing plasmids into the S. aureus ATCC 29213 background recapitulated the expected Cfr antibiogram, including resistance to linezolid, tiamulin, clindamycin, and florfenicol and susceptibility to tedizolid.
authors.affiliation: Trius Therapeutics, Inc., San Diego, California, USA jlocke@cidara.com.
authors.forename: Jeffrey B
authors.initials: JB
authors.lastname: Locke
authors.affiliation: Trius Therapeutics, Inc., San Diego, California, USA.
authors.forename: Douglas E
authors.initials: DE
authors.lastname: Zuill
authors.affiliation: Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska, USA.
authors.forename: Caitlyn R
authors.initials: CR
authors.lastname: Scharn
authors.affiliation: Eurofins Global Central Laboratory, Chantilly, Virginia, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Deane
authors.affiliation: Eurofins Global Central Laboratory, Chantilly, Virginia, USA.
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Sahm
authors.affiliation: Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska, USA.
authors.forename: Richard V
authors.initials: RV
authors.lastname: Goering
authors.affiliation: New York Presbyterian Hospital/Weill Cornell Medical Center, New York, New York, USA.
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Jenkins
authors.affiliation: Trius Therapeutics, Inc., San Diego, California, USA.
authors.forename: Karen J
authors.initials: KJ
authors.lastname: Shaw
mesh_terms: D000081:Acetamides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D002981:Clindamycin / Q000494:pharmacology; D004224:Diterpenes / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D018014:Gene Transfer Techniques; D006801:Humans; D000069349:Linezolid; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009518:New York; D010755:Organophosphates / Q000494:pharmacology; D010080:Oxazoles / Q000494:pharmacology; D023303:Oxazolidinones / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology; D013839:Thiamphenicol / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D000081:Acetamides; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C539943:CFR protein, Staphylococcus aureus; D004224:Diterpenes; D010755:Organophosphates; D010080:Oxazoles; D023303:Oxazolidinones; D002981:Clindamycin; C035534:florfenicol; C014224:tiamulin; D013839:Thiamphenicol; D000069349:Linezolid; C515040:tedizolid phosphate