title: Bone morphogenetic protein-9 induces PDLSCs osteogenic differentiation through the ERK and p38 signal pathways.
abstract: Periodontal ligament stem cells (PDLSCs) with bone morphogenic ability are used to treat diseases such as periodontitis. Their treatment potential is increased when used in combination with proteins that induce osteogenic differentiation. For example, bone morphogenetic protein-9 (BMP9) has been found to have potent osteogenic activity. In the present study, PDLSCs were isolated from human periodontal membrane and infected with recombinant adenoviruses expressing BMP9 (Ad-BMP9). Levels of osteogenic markers such as runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP), osteopontin (OPN), and osteocalcin (OCN) as well as mineralization ability were measured. The results showed that BMP9 promoted bone formation of PDLSCs. In other experiments, SB203580 and PD98059, which are inhibitors of p38 and ERK1/2, respectively, were used to determine if these kinases are involved in the osteogenic differentiation process. The resulting protein expression profiles and osteogenic markers of PDLSCs revealed that the mitogen-activated protein kinase (MAPK) signaling pathway might play an important role in the process of BMP9-induced osteogenic differentiation of PDLSCs.
authors.affiliation: 1. Chongqing key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing 401147, China ; 2. The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Ye
authors.affiliation: 1. Chongqing key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing 401147, China ; 2. The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, China.
authors.forename: Conghua
authors.initials: C
authors.lastname: Li
authors.affiliation: 1. Chongqing key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing 401147, China ; 2. The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, China.
authors.forename: Xuerong
authors.initials: X
authors.lastname: Xiang
authors.affiliation: 3. Department of Laboratory Medicine, M.O.E. Key Laboratory of Laboratory Medicine Diagnostics, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Chu
authors.initials: C
authors.lastname: Chen
authors.affiliation: 3. Department of Laboratory Medicine, M.O.E. Key Laboratory of Laboratory Medicine Diagnostics, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Ruyi
authors.initials: R
authors.lastname: Zhang
authors.affiliation: 1. Chongqing key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing 401147, China ; 2. The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
authors.affiliation: 1. Chongqing key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing 401147, China ; 2. The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Yu
authors.affiliation: 1. Chongqing key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing 401147, China ; 2. The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Wang
authors.affiliation: 1. Chongqing key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing 401147, China ; 2. The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.affiliation: 3. Department of Laboratory Medicine, M.O.E. Key Laboratory of Laboratory Medicine Diagnostics, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Shi
authors.affiliation: 3. Department of Laboratory Medicine, M.O.E. Key Laboratory of Laboratory Medicine Diagnostics, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Yaguang
authors.initials: Y
authors.lastname: Weng
mesh_terms: D000293:Adolescent; D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005419:Flavonoids / Q000494:pharmacology; D055427:Growth Differentiation Factor 2 / Q000378:metabolism*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D007150:Immunohistochemistry; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D053495:Osteopontin / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology*; D011725:Pyridines / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D005419:Flavonoids; D055427:Growth Differentiation Factor 2; D007093:Imidazoles; D011725:Pyridines; C493709:RUNX2 protein, human; D015675:Osteocalcin; D053495:Osteopontin; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D000469:Alkaline Phosphatase; C093642:SB 203580; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one
keywords: BMP9; MAPK; Osteogenesis.; Periodontal ligament stem cells