title: Distinctly different dynamics and kinetics of two steroid receptors at the same response elements in living cells.
abstract: Closely related transcription factors (TFs) can bind to the same response elements (REs) with similar affinities and activate transcription. However, it is unknown whether transcription is similarly orchestrated by different TFs bound at the same RE. Here we have compared the recovery half time (t1/2), binding site occupancy and the resulting temporal changes in transcription upon binding of two closely related steroid receptors, the androgen and glucocorticoid receptors (AR and GR), to their common hormone REs (HREs). We show that there are significant differences at all of these levels between AR and GR at the MMTV HRE when activated by their ligands. These data show that two TFs bound at the same RE can have significantly different modes of action that can affect their responses to environmental cues.
authors.affiliation: Department of Biosciences, University of Oslo, Oslo, Norway.
authors.forename: Hatice Z
authors.initials: HZ
authors.lastname: Nenseth
authors.affiliation: Department of Biosciences, University of Oslo, Oslo, Norway.
authors.forename: Xavier
authors.initials: X
authors.lastname: Dezitter
authors.affiliation: Department of Biosciences, University of Oslo, Oslo, Norway.
authors.forename: Martina
authors.initials: M
authors.lastname: Tesikova
authors.affiliation: Computational Imaging and Modeling Unit, Institut Pasteur, Paris, France.
authors.forename: Florian
authors.initials: F
authors.lastname: Mueller
authors.affiliation: Department of Biosciences, University of Oslo, Oslo, Norway.
authors.forename: Tove I
authors.initials: TI
authors.lastname: Klokk
authors.affiliation: Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institute of Health, Bethesda, Maryland, United States of America.
authors.forename: Gordon L
authors.initials: GL
authors.lastname: Hager
authors.affiliation: Department of Biosciences, University of Oslo, Oslo, Norway; Institute for Cancer Genetics and Informatics, Oslo University Hospital, Oslo, Norway.
authors.forename: Fahri
authors.initials: F
authors.lastname: Saatcioglu
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D047369:Chromatin Immunoprecipitation; D036681:Fluorescence Recovery After Photobleaching; D006801:Humans; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism*; D011965:Receptors, Glucocorticoid / Q000235:genetics / Q000378:metabolism*; D020218:Response Elements / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D011944:Receptors, Androgen; D011965:Receptors, Glucocorticoid