title: Transcriptional landscape of glomerular parietal epithelial cells.
abstract: Very little is known about the function of glomerular parietal epithelial cells (PECs). In this study, we performed genome-wide expression analysis on PEC-enriched capsulated vs. PEC-deprived decapsulated rat glomeruli to determine the transcriptional state of PECs under normal conditions. We identified hundreds of differentially expressed genes that mapped to distinct biologic modules including development, tight junction, ion transport, and metabolic processes. Since developmental programs were highly enriched in PECs, we characterized several of their candidate members at the protein level. Collectively, our findings confirm that PECs are multifaceted cells and help define their diverse functional repertoire.
authors.affiliation: Computational Medicine Core, Center for Lung Biology, Division of Pulmonary and Critical Care Medicine, University of Washington, Seattle, Washington, United States of America.
authors.forename: Sina A
authors.initials: SA
authors.lastname: Gharib
authors.affiliation: Division of Nephrology, University of Washington, Seattle, Washington, United States of America.
authors.forename: Jeffrey W
authors.initials: JW
authors.lastname: Pippin
authors.affiliation: Division of Nephrology, University of Tokyo, Tokyo, Japan.
authors.forename: Takamoto
authors.initials: T
authors.lastname: Ohse
authors.affiliation: Division of Nephrology, University of Washington, Seattle, Washington, United States of America.
authors.forename: Scott G
authors.initials: SG
authors.lastname: Pickering
authors.affiliation: Division of Nephrology, University of Washington, Seattle, Washington, United States of America.
authors.forename: Ronald D
authors.initials: RD
authors.lastname: Krofft
authors.affiliation: Division of Nephrology, University of Washington, Seattle, Washington, United States of America.
authors.forename: Stuart J
authors.initials: SJ
authors.lastname: Shankland
mesh_terms: D000818:Animals; D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D007678:Kidney Glomerulus / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D051381:Rats; D059467:Transcriptome