title: The influence of combined genotypes of the HLADRB1*1501 and CD24 single nucleotide polymorphism on disease severity of Iranian multiple sclerosis patients.
abstract: Multiple Sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system. It is a clinically heterogeneous disorder especially in terms of disease severity. Current investigations suggest that genes and gene-gene interactions not only influence on susceptibility to MS but also affect the disease severity. In this study, we investigated the contribution of the HLADRB1*1501 allele and single nucleotide polymorphism (SNP) in CD24 gene and also combined genotypes of the HLADRB1*1501 and CD24 SNP to disease severity in Iranian MS patients. We have reported previously that the HLA- DRB1*1501 allele and the CD24v/v genotype associated with disease susceptibility and some other studies proposed that HLA-DRB1*1501 allele be associated with MS severity. In this study, the results showed a significant difference in the Multiple Sclerosis Severity Score (MSSS) of the nine different genotypes (F=2.838, P=0.007). Subsequent analysis revealed a statistically significant difference in the MSSS between the MS patients who were carriers of HLA-DRB1*1501/1501 and those who were not carriers of HLA-DRB1*1501/1501 genotypes (P=0.04). Moreover, the MS patients carrying combined genotypes of the HLA- DRB1*1501/x-CD24 v/v had statistically severe disease than the patients who did not carry the HLA- DRB1*1501- CD24 v/v (P=0.047). In conclusion, our findings suggest that, HLA-DRB1*1501/1501 and bigenic genotypes of the HLA- DRB1*1501/x- CD24 v/v may influence on disease severity in Iranian MS patients.
authors.affiliation: Department of Neurology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.AND Department of Neurogenetics, Iranian Center of Neurological Research, Tehran, Iran. Mzamani@sina.tums.ac.ir.
authors.forename: Hossein Ali
authors.initials: HA
authors.lastname: Ghlichnia
authors.affiliation: Department of Neurogenetics, Iranian Center of Neurological Research, Tehran, Iran.AND Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Mzamani@sina.tums.ac.ir.
authors.forename: Abolghasem
authors.initials: A
authors.lastname: Kollaee
authors.affiliation: Department of Neurogenetics, Iranian Center of Neurological Research, Tehran, Iran. mzamani@tums.ac.ir.
authors.forename: Majid
authors.initials: M
authors.lastname: Gaffarpoor
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. mzamani@tums.ac.ir.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Movafagh
authors.affiliation: Department of Neurogenetics, Iranian Center of Neurological Research, Tehran, Iran. mzamani@tums.ac.ir.
authors.forename: Babak
authors.initials: B
authors.lastname: Ghlichnia
authors.affiliation: Department of Neurogenetics, Iranian Center of Neurological Research, Tehran, Iran.AND Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. mzamani@tums.ac.ir.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Zamani
mesh_terms: D000328:Adult; D051927:CD24 Antigen / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D059811:HLA-DRB1 Chains / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D015994:Incidence; D007492:Iran / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D051927:CD24 Antigen; D059811:HLA-DRB1 Chains; D004247:DNA