title: Protection of Wistar-Furth rats against postischaemic acute renal injury: role for nitric oxide and thromboxane?
abstract: The Wistar-Furth (WF) rat strain is usually used in models of full major histocompatibility complex-mismatched kidney transplantation. Because these rats have been demonstrated to be resistant to several models of chronic kidney disease, the aim of the present study was to investigate their potential resistance to renal ischaemia-reperfusion (I/R) injury compared with another strain, namely Wistar-Hanover (WH) rats. Anaesthetized male WH and WF rats were submitted to I/R by occlusion of the left renal artery and contralateral nephrectomy. Urine, blood and tissue samples were collected at different time points after I/R to evaluate renal function, inflammation and tubular injury, along with determination of nitric oxide synthase (NOS) expression and thromboxane A2 (TxA2 ) production. Post-ischaemic renal function was better preserved in WF than WH rats, as evidenced by reduced levels of creatininaemia, urinary neutrophil gelatinase-associated lipocalin excretion and proteinuria. In addition, WF rats had less intrarenal inflammation than WH rats after I/R injury. These observations were associated with maintenance of neuronal NOS expression, along with lower induction of inducible NOS expression in WF versus WH rats. Moreover, WF rats excreted a significantly lower amount of TxB2 . The results indicate that WF rats are more resistant to an I/R injury than WH rats in terms of renal function and inflammation. These observations are associated with differential regulation of intrarenal NOS expression, as well as a reduction in thromboxane production, which could contribute to a better outcome for the postischaemic kidney in WF rats.
authors.affiliation: Molecular Physiology Research Unit-Namur Research Institute for Life Sciences, Univerity of Namur, Namur, Belgium.
authors.forename: Viginie
authors.initials: V
authors.lastname: Voisin
authors.forename: Anne-Emilie
authors.initials: AE
authors.lastname: Declèves
authors.forename: Virginie
authors.initials: V
authors.lastname: Hubert
authors.forename: Vanessa
authors.initials: V
authors.lastname: Colombaro
authors.forename: Laetitia
authors.initials: L
authors.lastname: Giordano
authors.forename: Isabelle
authors.initials: I
authors.lastname: Habsch
authors.forename: Nadine
authors.initials: N
authors.lastname: Bouby
authors.forename: Denis
authors.initials: D
authors.lastname: Nonclercq
authors.forename: Nathalie
authors.initials: N
authors.lastname: Caron
mesh_terms: D000208:Acute Disease; D000818:Animals; D015232:Dinoprostone / Q000652:urine; D004195:Disease Models, Animal; D007668:Kidney / Q000098:blood supply / Q000276:immunology / Q000378:metabolism*; D007677:Kidney Function Tests; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000235:genetics; D018384:Oxidative Stress; D011920:Rats, Inbred WF; D060888:Real-Time Polymerase Chain Reaction; D015427:Reperfusion Injury / Q000276:immunology / Q000378:metabolism* / Q000517:prevention & control* / Q000652:urine; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013928:Thromboxane A2 / Q000096:biosynthesis*; D013929:Thromboxane B2 / Q000652:urine
chemical_list: D009569:Nitric Oxide; D013929:Thromboxane B2; D013928:Thromboxane A2; D019001:Nitric Oxide Synthase; D015232:Dinoprostone
keywords: Wistar-Furth rats; acute renal injury; nitric oxide synthase; renal ischaemia-reperfusion; thromboxane