title: [Effects of metformin on the survival of the SKOV-3 ovarian cancer cell line and the expression of genes encoding enzymes involved in O-Glcnacylation].
abstract: OBJECTIVE
  The aim of the study was to evaluate the cytotoxic effect of metformin on the ovarian cancer cells SKOV-3 and analyze the impact of this compound on the expression of genes coding for O-GlcNAc cycling enzymes, i.e. O-GlcNAc transferase (OGT) and -N-acetylglucosaminidase (OGA).
  
  
  METHODS
  Viability and proliferation of control cells and cells treated with metformin were evaluated by MTT test and trypan blue staining. OGT and OGA mRNA expressions analysis was performed using real-time PCR method.
  
  
  RESULTS
  A metformin concentration-dependent decrease of SKOV-3 cell viability was observed. The IC50 parameter for metformin cytotoxicity was 14 mM. The SKOV-3 cell doubling time was 45 hours. The cell population treated with 10 mM metformin did not double even after 72 hours. There was no significant difference in mRNA level of OGA between control cells and cells treated with metformin. The OGT mRNA level was significantly higher in cells treated with metforrhin for 24 hours as compared to the control cells. The increase of OGT mRNA was dependent on time of incubation. Cells treated with metformin for 48 hour showed higher expression of OGT than cells treated for 24 hours.
  
  
  CONCLUSIONS
  Antiproliferative activity of metformin suggests that this compound may be considered as a candidate for potential chemotherapeutic agent. However taking into account its impact on the expression of O-GlcNAc transferase, further studies on the molecular mechanism of metformin action are necessary
authors.forename: Aneta
authors.initials: A
authors.lastname: Rogalska
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wójcik-Krowiranda
authors.forename: Ewa
authors.initials: E
authors.lastname: Forma
authors.forename: Piotr
authors.initials: P
authors.lastname: Ciesielski
authors.forename: Andrzej
authors.initials: A
authors.lastname: Bieńkiewicz
authors.forename: Magdalena
authors.initials: M
authors.lastname: Brys
authors.forename: Anna
authors.initials: A
authors.lastname: Krzeslak
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Marczak
mesh_terms: D000328:Adult; D000970:Antineoplastic Agents / Q000494:pharmacology; D002460:Cell Line / Q000187:drug effects; D003603:Cytotoxins / Q000494:pharmacology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D008687:Metformin / Q000494:pharmacology*; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000473:pathology; D001619:beta-N-Acetylhexosaminidases / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D003603:Cytotoxins; D008687:Metformin; D017351:N-Acetylglucosaminyltransferases; C449880:O-GlcNAc transferase; D001619:beta-N-Acetylhexosaminidases