title: Ameliorating effects of CAPE on oxidative damage caused by pneumoperitoneum in rat lung tissue.
abstract: We investigated the biochemical and histopathological effects of caffeic acid phenethyl ester (CAPE) against oxidative stress causing lung injury induced by pneumoperitoneum. Twenty-eight rats were selected at random and seven rats were assigned to each of the following groups. The control group (S) was subjected to a sham operation without pneumoperitoneum. The other groups were subjected to CO2 pneumoperitoneum 15 mmHg for 60 min. The laparoscopy group (L) had no additional drugs administered, the laparoscopy + alcohol (LA) group had 1 ml of 70% ethyl alcohol administered 1 h before the desufflation period, and the laparoscopy + CAPE (LC) group had CAPE administered at 10 μmol/kg 1 h before the desufflation period. The total oxidative status levels of lung and plasma were significantly increased in the LA group as compared with the LC and S group. When the LC group was compared with the L group, there was a decrease in the level of total oxidant status and increase in the levels of total antioxidant status and paraoxonase in lung tissue. The level of total antioxidative status in the S group was increased compared with the L group in lung tissue and bronchoalveolar lavage fluid. TNF-α and IL-6 were found significantly elevated in the L group compared with the LC and S groups in bronchoalveolar lavage fluid. There was a similar increase in plasma levels of IL-6. These results were supported by histopathological examination. CAPE was found to considerably reduce oxidative stress and inflammation induced by pneumoperitoneum.
authors.affiliation: Department of Anesthesiology, Faculty of Medicine, Mustafa Kemal University Hatay, Turkey.
authors.forename: Isil
authors.initials: I
authors.lastname: Davarci
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Mustafa Kemal University Hatay, Turkey.
authors.forename: Harun
authors.initials: H
authors.lastname: Alp
authors.affiliation: Department of Pathology, Faculty of Medicine, Mustafa Kemal University Hatay, Turkey.
authors.forename: Tumay
authors.initials: T
authors.lastname: Ozgur
authors.affiliation: Department of Anesthesiology, Faculty of Medicine, Mustafa Kemal University Hatay, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Karcioglu
authors.affiliation: Department of Anesthesiology, Faculty of Medicine, Mustafa Kemal University Hatay, Turkey.
authors.forename: Kasim
authors.initials: K
authors.lastname: Tuzcu
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Dicle University Diyarbakır, Turkey.
authors.forename: Osman
authors.initials: O
authors.lastname: Evliyaoglu
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Mustafa Kemal University Hatay, Turkey.
authors.forename: Sedat
authors.initials: S
authors.lastname: Motor
authors.affiliation: Department of Thoracic Surgery, Faculty of Medicine, Mustafa Kemal University Hatay, Turkey.
authors.forename: Tulin
authors.initials: T
authors.lastname: Durgun Yetim
keywords: Caffeic acid phenethyl ester; bronchoalveolar lavage; lung; pneumoperitoneum; rat