title: Effects of SertaSil on wound healing in the rat.
abstract: OBJECTIVE
  SertaSil is a novel product for the topical management of wound exudate. The purpose of this study was to evaluate the ability of SertaSil to promote wound healing in a pre-clinical wound model.
  
  
  METHODS
  An aseptic wound was induced in rats by administering 1ml 10% calcium chloride solution into the subcutaneous layer under local anaesthesia. Following opening of the abscess, animals were divided into a control group (no treatment) and either SertaSil or Gentaxane, which were applied topically to the wound every 24 hours until a clean wound was achieved, that is, free from necrosis, pus and fibrinogenous thickenings.
  
  
  RESULTS
  Rats (n=15 per group) receiving SertaSil reached the clean wound stage in 3.0 ± 0.4 days compared to 7.0 ± 0.4 days for Gentaxane and 10.0 ± 0.4 days for the control. Time to wound closure was 13.9 ± 0.3 days for SertaSil, 18.7 ± 0.6 days for Gentaxane, and 23.0 ± 0.4 days for the control. The surface area of the wounds were measured at day 1 and day 13. At day 1, the wound surface areas (mm2) were similar in all three groups (157.4 ± 8.9), but at day 13 the SertaSil group had significantly smaller wound areas (5.2 ± 1.7) compared to the Gentaxane (38.0 ± 1.5) and control groups (95.7 ± 11.3). The study was conducted in young rats that are still growing and gaining weight. At day 19, only the rats receiving SertaSil exhibited a weight increase (271 ± 5 g) indicating good recovery, whereas rats receiving Gentaxane did not gain weight (249 ± 5 g) and rats in the control group lost weight (242 ± 16 g).
  
  
  CONCLUSIONS
  The study found that SertaSil reduced the time to reaching a clean wound by 60% compared to Gentaxane and promoted faster wound closure and better recovery. These findings suggest that SertaSil may be valuable for use in the treatment of wounds in patients.
authors.affiliation: Professor, Shupik National Medical Academy of Postgraduate Education, Kiev, Ukraine.
authors.forename: O
authors.initials: O
authors.lastname: Bilyayeva
authors.forename: V V
authors.initials: VV
authors.lastname: Neshta
authors.forename: A
authors.initials: A
authors.lastname: Golub
authors.forename: F
authors.initials: F
authors.lastname: Sams-Dodd
mesh_terms: D000038:Abscess / Q000139:chemically induced / Q000188:drug therapy*; D000287:Administration, Topical; D000818:Animals; D002122:Calcium Chloride; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D005122:Exudates and Transudates / Q000187:drug effects; D005839:Gentamicins / Q000008:administration & dosage; D008297:Male; D010447:Peptide Hydrolases / Q000008:administration & dosage; D051381:Rats; D012822:Silicon Dioxide / Q000008:administration & dosage; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D005839:Gentamicins; C000592423:SertaSil; D012822:Silicon Dioxide; D010447:Peptide Hydrolases; D002122:Calcium Chloride
keywords: exudate; healing; inflammation; pre-clinical; wound care