title: Isolation and activity of the promoters for STAT1 and 2 in Atlantic salmon Salmo salar.
abstract: Signal Transducer and Activator of Transcription (STAT) 1 and 2 molecules are part of the interferon (IFN) type I and type II (γIFN) signalling pathways, key pathways in the innate immune response. Genomic sequence regions upstream from the 5-prime Salmo salar ORFs were obtained and shown to have functional activity through their incorporation into luciferase reporter constructs and subsequent activation by salmonid alpha virus (SAV). The STAT1 and STAT2 putative promoter regions were also induced by co-transfected plasmids expressing γIFN and IFN type I respectively. Two IFN-induced gene regulatory motifs (GAAANN) associated in a complete Interferon Stimulating Response Element (ISRE) were identified in the STAT1 putative promoter sequence and several GAS elements conforming to Boehm's consensus TTNCNNNAA. Sixteen IFN-induced gene regulatory motifs (GAAANN) could be identified in the STAT2 putative promoter region but no Boehm's GAS element nor ISRE. A palindromic sequence that conforms to Decker's consensus GAS element TTCNNN(N)GAA was identified. The reporter constructs generated here may prove an additional tool for refining knowledge on interferon signalling in fish and the inhibition of such by some fish viral pathogens.
authors.affiliation: Marine Scotland, 375 Victoria Road, Aberdeen, AB11 9DB, UK. Electronic address: Catherine.Collins@scotland.gsi.gov.uk.
authors.forename: Catherine
authors.initials: C
authors.lastname: Collins
authors.affiliation: Marine Scotland, 375 Victoria Road, Aberdeen, AB11 9DB, UK.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Ganne
authors.affiliation: Marine Scotland, 375 Victoria Road, Aberdeen, AB11 9DB, UK.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Collet
mesh_terms: D000818:Animals; D001483:Base Sequence; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D007370:Interferon Type I / Q000235:genetics / Q000378:metabolism; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011401:Promoter Regions, Genetic; D050794:STAT1 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D050795:STAT2 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D020317:Salmo salar / Q000235:genetics* / Q000378:metabolism; D014162:Transfection / Q000662:veterinary
chemical_list: D029941:Fish Proteins; D007370:Interferon Type I; D050794:STAT1 Transcription Factor; D050795:STAT2 Transcription Factor; D007371:Interferon-gamma
keywords: Promoter; Reporter system; STAT1; STAT2; Viral infection