title: Leishmania amazonensis infection impairs dendritic cell migration from the inflammatory site to the draining lymph node.
abstract: BACKGROUND
  In vitro studies show that Leishmania infection decreases the adhesion of inflammatory phagocytes to connective tissue by a mechanism dependent on the modulation of integrin function. However, we know little about the influence of this reduction in leukocyte adhesion on parasite dissemination from the infection site.
  
  
  METHODS
  In this work, we used a model of chronic peritonitis induced by thioglycollate to study the effect of L. amazonensis infection on the ability of inflammatory phagocyte populations to migrate from an inflammatory site to the draining lymph node. Uninfected or Leishmania-infected thioglycollate-elicited peritoneal exudate cells were transferred from C57BL/6 to BALB/c mice or from Ly5.1+ to Ly5.1- mice. The transferred cells were injected into the peritoneal cavity and tracked to the draining lymph node.
  
  
  RESULTS
  Migrating cells corresponded to approximately 1% of the injected leukocytes. The proportion of migrating CD11b+CD11c+ (myeloid dendritic cell) was lower after incubation with Leishmania (1.3 to 2.6 times lower in the experiments using C57BL/6 to BALB/c animals and 2.7 to 3.4 times lower in the experiments using Ly5.1+ to Ly5.1- animals) than after leukocyte incubation with medium alone (P < 0.01). There was no consistent decrease in the migration of CD11b+F4/80+ (macrophage) or SSChi GR-1+ (neutrophil) populations.
  
  
  CONCLUSIONS
  Coincubation with Leishmania changes the migratory pattern of dendritic cells in vivo. Such changes in dendritic cell migration may be associated with immunological events that maintain inflammation at the sites of infection.
authors.forename: Micely D R
authors.initials: MD
authors.lastname: Hermida
authors.forename: Priscila G
authors.initials: PG
authors.lastname: Doria
authors.forename: Angela M P
authors.initials: AM
authors.lastname: Taguchi
authors.forename: José O
authors.initials: JO
authors.lastname: Mengel
authors.affiliation: Centro de Pesquisas Gonçalo Moniz, Oswaldo Cruz Foundation, Fiocruz, LPBI, Salvador, Brazil. wluis@bahia.fiocruz.br.
authors.forename: Washington  L C
authors.initials: W
authors.lastname: dos-Santos
mesh_terms: D000818:Animals; D002465:Cell Movement; D003713:Dendritic Cells / Q000276:immunology / Q000469:parasitology*; D007249:Inflammation; D007891:Leishmania; D007896:Leishmaniasis / Q000382:microbiology*; D007962:Leukocytes / Q000166:cytology; D008198:Lymph Nodes / Q000469:parasitology*; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000166:cytology / Q000276:immunology; D010586:Phagocytes / Q000166:cytology; D010587:Phagocytosis