title: Insecticidal metabolites from the rhizomes of Veratrum album against adults of Colorado potato beetle, Leptinotarsa decemlineata.
abstract: The dried rhizomes of Veratrum album were individually extracted with CHCl3 , acetone, and NH4 OH/benzene to test the toxic effects against the Colorado potato beetle, Leptinotarsa decemlineata, which is an important agricultural pest. Fifteen compounds in various amounts were isolated from the extracts using column and thin-layer chromatography. The chemical structures of 14 compounds were characterized as octacosan-1-ol (1), β-sitosterol (2), stearic acid (3), diosgenin (4), resveratrol (5), wittifuran X (6), oxyresveratrol (7), β-sitosterol 3-O-β-D-glucopyranoside (8), diosgenin 3-O-α-L-rhamnopyranosyl-(1 → 2)-β-D-glucopyronoside (9), oxyresveratrol 3-O-β-D-glucopyranoside (10), jervine (11), pseudojervine (13), 5,6-dihydro-1-hydroxyjervine (14), and saccharose (15) using UV, IR, MS, (1) H- and (13)C-NMR, and 2D-NMR spectroscopic methods. However, the chemical structure of 12, an oligosaccharide, has not fully been elucidated. Compounds 4, 6, 9, and 10 were isolated from V. album rhizomes for the first time in the current study. The toxic effects of three extracts (acetone, CHCl3 , and NH4 OH/benzene) and six metabolites, 2, 2+4, 5, 7, 8, and 11, were evaluated against the Colorado potato beetle. The assay revealed that all three extracts, and compounds 7, 8, and 11 exhibited potent toxic effects against this pest. This is the first report on the evaluation of the toxic effects of the extracts and secondary metabolites of V. album rhizomes against L. decemlineata. Based on these results, it can be concluded that the extracts can be used as natural insecticides.
authors.affiliation: Agri İbrahim Cecen University, Health Services Vocational School, Department of Paramedic, TR-04100, Agri.
authors.forename: Tuba
authors.initials: T
authors.lastname: Aydin
authors.forename: Ahmet
authors.initials: A
authors.lastname: Cakir
authors.forename: Cavit
authors.initials: C
authors.lastname: Kazaz
authors.forename: Neslihan
authors.initials: N
authors.lastname: Bayrak
authors.forename: Yasin
authors.initials: Y
authors.lastname: Bayir
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Taşkesenligil
mesh_terms: D000818:Animals; D002855:Chromatography, Thin Layer; D001517:Coleoptera / Q000187:drug effects*; D007306:Insecticides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D027343:Rhizome / Q000737:chemistry; D014703:Veratrum / Q000737:chemistry* / Q000378:metabolism
chemical_list: D007306:Insecticides; D010936:Plant Extracts
keywords: Diosgenin; Insecticidal activity; Jervine; Leptinotarsa decemlineata; Veratrum album