title: Correlation study on adiponectin gene SNP45 and long-term oxidative stress in patients with diabetes and carotid atherosclerosis.
abstract: The aim of the present study was to investigate the correlation between the adiponectin gene single nucleotide polymorphism (SNP)45 T/G and long-term oxidative stress in type II diabetes mellitus (T2DM) patients with carotid atherosclerosis. Patients with T2DM were divided into non-carotid atherosclerosis and carotid atherosclerosis groups, which were then subsequently divided into TT and TG + GG groups according to the adiponectin SNP45 T/G genotypes. Enzyme-linked immunosorbent assay,TaqMan probe quantitative polymerase chain reaction (PCR), PCR-TaqMan, color Doppler and other methods were used to determine the adiponectin levels, gene polymorphisms, acquired mitochondrial DNA (mtDNA) A3243G somatic cell mutation rates and the carotid intima-media thickness. The somatic cell mutation rate of acquired mtDNA A3243A/G in the T2DM carotid atherosclerosis group was significantly higher compared with the group without carotid atherosclerosis. In addition, the acquired mtDNA A3243A/G somatic cell mutation rate in the T2DM carotid atherosclerosis group with the adiponectin gene SNP45 TT genotype was significantly lower compared with the SNP45 TG/GG genotype group. T2DM combined with carotid atherosclerosis was associated with long-term oxidative stress. In addition, adiponectin gene SNP45 T/G was associated with increased mtDNA A3243A/G somatic mutation rates in T2DM patients with carotid atherosclerosis. Therefore, adiponectin gene polymorphisms may lead to diabetes atherosclerosis through oxidative stress.
authors.affiliation: Department of Endocrinology, The Affiliated Hospital of Yanbian University, Yanji, Jilin 133000, P.R. China.
authors.forename: Lianshan
authors.initials: L
authors.lastname: Piao
authors.affiliation: Department of Endocrinology, The Affiliated Hospital of Yanbian University, Yanji, Jilin 133000, P.R. China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Endocrinology, The Affiliated Hospital of Yanbian University, Yanji, Jilin 133000, P.R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
keywords: adiponectin single nucleotide polymorphism 45 T/G; carotid intima-media thickness; long-term oxidative stress; type II diabetes