title: Carnosine inhibits the proliferation of human gastric cancer SGC-7901 cells through both of the mitochondrial respiration and glycolysis pathways.
abstract: Carnosine, a naturally occurring dipeptide, has been recently demonstrated to possess anti-tumor activity. However, its underlying mechanism is unclear. In this study, we investigated the effect and mechanism of carnosine on the cell viability and proliferation of the cultured human gastric cancer SGC-7901 cells. Carnosine treatment did not induce cell apoptosis or necrosis, but reduced the proliferative capacity of SGC-7901 cells. Seahorse analysis showed SGC-7901 cells cultured with pyruvate have active mitochondria, and depend on mitochondrial oxidative phosphorylation more than glycolysis pathway for generation of ATP. Carnosine markedly decreased the absolute value of mitochondrial ATP-linked respiration, and reduced the maximal oxygen consumption and spare respiratory capacity, which may reduce mitochondrial function correlated with proliferative potential. Simultaneously, carnosine also reduced the extracellular acidification rate and glycolysis of SGC-7901 cells. Our results suggested that carnosine is a potential regulator of energy metabolism of SGC-7901 cells both in the anaerobic and aerobic pathways, and provided a clue for preclinical and clinical evaluation of carnosine for gastric cancer therapy.
authors.affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education, Attardi Institute of Mitochondrial Biomedicine and Zhejiang Provincial Key Laboratory of Medical Genetics, College of Laboratory Medicine and Life sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education, Attardi Institute of Mitochondrial Biomedicine and Zhejiang Provincial Key Laboratory of Medical Genetics, College of Laboratory Medicine and Life sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Yang
authors.affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education, Attardi Institute of Mitochondrial Biomedicine and Zhejiang Provincial Key Laboratory of Medical Genetics, College of Laboratory Medicine and Life sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education, Attardi Institute of Mitochondrial Biomedicine and Zhejiang Provincial Key Laboratory of Medical Genetics, College of Laboratory Medicine and Life sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Shi
authors.affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education, Attardi Institute of Mitochondrial Biomedicine and Zhejiang Provincial Key Laboratory of Medical Genetics, College of Laboratory Medicine and Life sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ouyang
authors.affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education, Attardi Institute of Mitochondrial Biomedicine and Zhejiang Provincial Key Laboratory of Medical Genetics, College of Laboratory Medicine and Life sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Yueyang
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education, Attardi Institute of Mitochondrial Biomedicine and Zhejiang Provincial Key Laboratory of Medical Genetics, College of Laboratory Medicine and Life sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Lu
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D002336:Carnosine / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019069:Cell Respiration; D002470:Cell Survival / Q000187:drug effects; D004272:DNA, Mitochondrial; D004734:Energy Metabolism / Q000187:drug effects; D005110:Extracellular Space / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects*; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D011773:Pyruvates / Q000378:metabolism; D013274:Stomach Neoplasms / Q000378:metabolism*; D014410:Tumor Stem Cell Assay
chemical_list: D004272:DNA, Mitochondrial; D011773:Pyruvates; D019344:Lactic Acid; D002336:Carnosine; D000255:Adenosine Triphosphate