title: Single nucleotide polymorphism variants within tva and tvb receptor genes in Chinese chickens.
abstract: Avian leukosis is an immunosuppressive neoplastic disease caused by avian leukosis viruses (ALV), which causes tremendous economic losses in the worldwide poultry industry. The susceptibility or resistance of chicken cells to subgroup A ALV and subgroup B, D, and E ALV are determined by the receptor genes tumor virus locus A (tva) and tumor virus locus B (tvb), respectively. Four genetic resistant loci (tva(r1), tva(r2), tva(r3), and tva(r4)) in tva receptor gene and a genetic resistant locus tvb(r) in the tvb receptor gene have been identified in inbred lines of White Leghorn. To evaluate the genetic resistance to subgroup A, B, D, and E ALV, genetic variations within resistant loci in tva and tvb genes were screened in Chinese local chicken breeds and commercial broiler lines. Here, the heterozygote tva(s1/r1) and the resistant genotype tva(r2/r2), tva(r3/r3), and tva(r4/r4) were detected in Chinese chickens by direct sequencing. The heterozygote tva(s1/r1) was detected in Huiyang Bearded chicken (HYBC), Rizhaoma chicken, and commercial broiler line 13 to 15 (CB13 to CB15), with the frequencies at 0.08, 0.18, 0.17, 0.25, and 0.15, respectively. The resistant genotype tva(r2/r2) was detected in Jiningbairi chicken (JNBRC), HYBC, and CB15, with the frequencies at 0.03, 0.08, and 0.06, respectively, whereas tva(r3/r3) and tva(r4/r4) were detected in 19 and 17 of the 25 Chinese chickens tested, with the average frequencies at 0.13 and 0.20, respectively. Furthermore, the resistant genotype tvb(r/r) was detected in JNBRC, CB07, CB12, CB14, and CB15 by pyrosequencing assay, with the frequencies at 0.03, 0.03, 0.11, 0.09, and 0.15, respectively. These results demonstrated that the potential for genetic improvement of resistance to subgroup A, B, D, and E ALV were great both in Chinese local chickens and commercial broilers. This study provides valuable insight into the selective breeding for chickens genetically resistant to ALV.
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, P. R. China Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, P. R. China.
authors.forename: C T
authors.initials: CT
authors.lastname: Liao
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, P. R. China.
authors.forename: S Y
authors.initials: SY
authors.lastname: Chen
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, P. R. China.
authors.forename: W G
authors.initials: WG
authors.lastname: Chen
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, P. R. China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, P. R. China.
authors.forename: B L
authors.initials: BL
authors.lastname: Sun
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, P. R. China.
authors.forename: H X
authors.initials: HX
authors.lastname: Li
authors.affiliation: USDA, Agriculture Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI 48823.
authors.forename: H M
authors.initials: HM
authors.lastname: Zhang
authors.affiliation: Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P. R. China.
authors.forename: H
authors.initials: H
authors.lastname: Qu
authors.affiliation: Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P. R. China.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P. R. China qmx@scau.edu.cn shudm@263.net.
authors.forename: D M
authors.initials: DM
authors.lastname: Shu
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, P. R. China Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, P. R. China Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong Provincial Department of Science and Technology, Guangzhou 510642, P. R. China qmx@scau.edu.cn shudm@263.net.
authors.forename: Q M
authors.initials: QM
authors.lastname: Xie
mesh_terms: D000818:Animals; D001353:Avian Leukosis / Q000235:genetics* / Q000821:virology; D001354:Avian Leukosis Virus / Q000502:physiology; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism; D002645:Chickens / Q000235:genetics*; D002681:China; D060467:Disease Resistance / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020641:Polymorphism, Single Nucleotide; D011201:Poultry Diseases / Q000235:genetics* / Q000821:virology; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics* / Q000378:metabolism; D011991:Receptors, Virus / Q000235:genetics* / Q000378:metabolism
chemical_list: D030161:Avian Proteins; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D011991:Receptors, Virus; C088745:Tva receptor
keywords: Chinese chicken; avian leukosis virus; genetic resistance; tva receptor gene; tvb receptor gene