title: Rho GTPase-activating protein 35 rs1052667 polymorphism and osteosarcoma risk and prognosis.
abstract: The Rho GTPase-activating protein 35 (ARHGAP35), an important Rho family GTPase-activating protein, may be associated with tumorigenesis of some tumors. Here, we investigated the relationship between an important polymorphic variant at 3'-UTR of this gene (rs1052667) and osteosarcoma risk and prognosis. This hospital-based case-control study, including 247 osteosarcoma patients and 428 age-, sex-, and race-matched healthy controls, was conducted in Guangxi population. Genotypes were tested using TaqMan PCR technique. We found a significant difference in the frequency of rs1052667 genotypes between cases and controls. Compared with the homozygote of rs1052667 C alleles (rs1052667-CC), the genotypes with rs1052667 T alleles (namely, rs1052667-CT or -TT) increased osteosarcoma risk (odds ratios: 2.41 and 7.35, resp.). Moreover, rs1052667 polymorphism was correlated with such pathological features of osteosarcoma as tumor size, tumor grade, and tumor metastasis. Additionally, this polymorphism also modified the overall survival and recurrence-free survival of osteosarcoma cases. Like tumor grade, ARHGAP35 rs1052667 polymorphism was an independent prognostic factor influencing the survival of osteosarcoma. These results suggest that ARHGAP35 rs1052667 polymorphism may be associated with osteosarcoma risk and prognosis.
authors.affiliation: Department of Orthopaedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, China ; Research Center for Regenerative Medicine, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, China.
authors.forename: Jinmin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Research Center for Regenerative Medicine, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, China ; Center for Education Evaluation & Faculty Development, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xu
authors.affiliation: Research Center for Regenerative Medicine, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, China ; Division of Spinal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, China.
authors.forename: Maolin
authors.identifier: 0000-0002-9154-9589
authors.initials: M
authors.lastname: He
authors.affiliation: Research Center for Regenerative Medicine, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, China ; Division of Spinal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Research Center for Regenerative Medicine, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, China ; Division of Spinal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wu
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D000483:Alleles; D002681:China / Q000453:epidemiology; D018572:Disease-Free Survival; D005260:Female; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D011110:Polymorphism, Genetic; D012097:Repressor Proteins / Q000235:genetics*; D015996:Survival Rate
chemical_list: D020413:3' Untranslated Regions; C074560:ARHGAP35 protein, human; D020662:Guanine Nucleotide Exchange Factors; D009363:Neoplasm Proteins; D012097:Repressor Proteins