title: In vitro three-dimensional bone tissue models: from cells to controlled and dynamic environment.
abstract: Most of our knowledge of bone cell physiology is derived from experiments carried out in vitro on polystyrene substrates. However, these traditional monolayer cell cultures do not reproduce the complex and dynamic three-dimensional (3D) environment experienced by cells in vivo. Thus, there is a growing interest in the use of 3D culture systems as tools for understanding bone biology. These in-vitro-engineered systems, less complex than in vivo models, should ultimately recapitulate and control the main biophysical, biochemical, and biomechanical cues that define the in vivo bone environment, while allowing their monitoring. This review focuses on state-of-the-art and the current advances in the development of 3D culture systems for bone biology research. It describes more specifically advantages related to the use of such systems, and details main characteristics and challenges associated with its three main components, that is, scaffold, cells, and perfusion bioreactor systems. Finally, future challenges for noninvasive imaging technologies are addressed.
authors.affiliation: 1 Laboratoire de Biologie du Tissu Osseux, Institut National de la Santé et de la Recherche Médicale-U1059, Université de Lyon-Université Jean Monnet , Saint-Etienne, France .
authors.forename: Guenaelle
authors.initials: G
authors.lastname: Bouet
authors.forename: David
authors.initials: D
authors.lastname: Marchat
authors.forename: Magali
authors.initials: M
authors.lastname: Cruel
authors.forename: Luc
authors.initials: L
authors.lastname: Malaval
authors.forename: Laurence
authors.initials: L
authors.lastname: Vico
mesh_terms: D000818:Animals; D019149:Bioreactors; D001842:Bone and Bones / Q000502:physiology*; D002477:Cells / Q000378:metabolism*; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008954:Models, Biological; D054457:Tissue Scaffolds / Q000737:chemistry