title: The effects of siRNA-silenced TRPC6 on podocyte autophagy and apoptosis induced by AngII.
abstract: OBJECTIVE
  the objective of this article is to evaluate the role of siRNA-silenced TRPC6 on podocyte autophagy and apoptosis induced by AngII.
  
  
  METHODS
  mouse podocyte cell lines were cultured in vitro. The apoptosis rates of each group were detected using flow cytometry. The expression of LC3-II protein and changes in distribution were detected by confocal laser, and the western blot protocol was employed for detection of protein expression of LC3-II.
  
  
  RESULTS
  AngII-injured podocyte had a significant increase in apoptosis, while silencing TRPC6 could decrease the apoptosis induced by AngII. Autophagy remarkably increased after AngII injury. While silencing TRPC6 stabilized the autophagy expression, AngII could activate the autophagy of podocyte. Autophagy-associated protein LC3-II expression increased after AngII injury. The LC3-II mRNA and the protein level could be down regulated by 3-MA. The silencing of TRPC6 could stabilize the autophagy expression.
  
  
  CONCLUSIONS
  the data suggest that AngII can lead to podocyte injury. Autophagy may have beneficial effects in preventing the progression of proteinuria. This study provides some new clues for further exploring the occurrence of podocyte injury and the development mechanism of proteinuria.
authors.affiliation: Department of Pediatrics, Guangzhou First People's Hospital, affiliate to Guangzhou Medical University, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Shengyou
authors.affiliation: Department of Pediatrics, Guangzhou First People's Hospital, affiliate to Guangzhou Medical University, China yuli828@yeah.net.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001343:Autophagy / Q000187:drug effects*; D015153:Blotting, Western; D002460:Cell Line; D005434:Flow Cytometry; D020868:Gene Silencing / Q000187:drug effects; D051379:Mice; D018613:Microscopy, Confocal; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D010588:Phagosomes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D050199:Podocytes / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D034741:RNA, Small Interfering / Q000378:metabolism*; D013194:Staining and Labeling; D050052:TRPC Cation Channels / Q000378:metabolism*; D000074287:TRPC6 Cation Channel; D014162:Transfection
chemical_list: C480860:Map1lc3b protein, mouse; D008869:Microtubule-Associated Proteins; D034741:RNA, Small Interfering; D050052:TRPC Cation Channels; D000074287:TRPC6 Cation Channel; C492146:Trpc6 protein, mouse; D000804:Angiotensin II
keywords: AngII; Podocyte; TRPC6; apoptosis; autophagy