title: Curcumin-functionalized silk materials for enhancing adipogenic differentiation of bone marrow-derived human mesenchymal stem cells.
abstract: Curcumin, a natural phenolic compound derived from the plant Curcuma longa, was physically entrapped and stabilized in silk hydrogel films, and its influence on human bone marrow-derived mesenchymal stem cells (hBMSC) was assessed related to adipogenic differentiation. The presence of curcumin significantly reduced the silk gelation time and changed the porous morphology of gel matrix, but did not change the formation of the silk beta-sheet structure. Based on spectrofluorimetric analysis, curcumin most likely interacted with hydrophobic residues in silk, interacting with the beta-sheet domains formed in the hydrogels. The antioxidant activity of silk film-associated curcumin remained functional over at least one month in both the dry and hydrated state. Negligible curcumin was released from silk hydrogel films over 48 h incubation in aqueous solution. For hBMSC cultured on silk films containing more than 0.25 mg ml(-1) curcumin, cell proliferation was inhibited, while adipogenesis was significantly promoted based on transcripts as well as Oil Red O staining. When hBMSC were cultured in media containing free curcumin, both proliferation and adipogenesis of hBMSC were inhibited when curcumin concentrations exceeded 5 μM, which is more than 1000 times higher than the level of curcumin released from the films in aqueous solution. Thus, silk film-associated curcumin exhibited different effects on hBMSC proliferation and differentiation compared with curcumin in solution.
authors.affiliation: National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China; Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Li
authors.affiliation: National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Luo
authors.affiliation: National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China.
authors.forename: Zhaozhu
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA; Department of Chemistry, Western Washington University, Bellingham, WA 98225, USA.
authors.forename: Amanda R
authors.initials: AR
authors.lastname: Murphy
authors.affiliation: National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China; Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA. Electronic address: wangxiaoqin@suda.edu.cn.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Wang
authors.affiliation: National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China; Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA. Electronic address: david.kaplan@tufts.edu.
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology*; D050156:Adipogenesis / Q000502:physiology*; D001672:Biocompatible Materials / Q000138:chemical synthesis; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D003474:Curcumin / Q000737:chemistry / Q000493:pharmacokinetics*; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D047011:Silk / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D047011:Silk; D003474:Curcumin
keywords: Adipogenesis; Curcumin; Differentiation; Mesenchymal stem cells; Silk fibroin