title: Immunohistochemical expression of MPO, CD163 and VEGF in inflammatory cells in acute respiratory distress syndrome: a case report.
abstract: Acute respiratory distress syndrome (ARDS) is a serious medical condition occurring in patients with polytrauma, pulmonary or non-pulmonary sepsis, pneumonia and many other circumstances. It causes inflammation of the lung parenchyma leading to impaired gas exchange with a systemic release of inflammatory mediators, causing consequential lung tissue injury, hypoxemia and frequently multiple organ failure. The aim of current study was to describe expression of inflammatory markers (myeloperoxidase, CD163 and vascular endothelial growth factor) by the cells in acute phase of ARDS. The lung samples of a 20-year-old man who had suffered a serious motorbike accident were obtained for histological examination. He died on the seventh day as a consequence of respiratory failure. Our results imply that expression of CD163 was restricted to activated alveolar macrophages and monocytes. Immunopositivityof MPO was observed in neutrophil granulocytes within lung alveoli and lung blood vessels. Myeloperoxidase positivity was observed in alveolar macrophages, too. Vascular endothelial growth factor was expressed in cytoplasm of neutrophil granulocytes, monocytes, small-sized alveolar macrophages and type II pneumocytes localized mostly inside lung alveoli. On the contrary, no positivity was observed in lung endothelial cells of blood vessels.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Pavol Jozef Šafárik University Šrobárova 2, Košice, Slovak Republic.
authors.forename: Milan
authors.initials: M
authors.lastname: Maretta
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Pavol Jozef Šafárik University Šrobárova 2, Košice, Slovak Republic.
authors.forename: Stefan
authors.initials: S
authors.lastname: Toth
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Pavol Jozef Šafárik University Šrobárova 2, Košice, Slovak Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Jonecova
authors.affiliation: International Clinical Research Center, St. Anne's University Hospital and Masaryk University Pekarska 53, 656 91 Brno, Czech Republic.
authors.forename: Peter
authors.initials: P
authors.lastname: Kruzliak
authors.affiliation: Department of Medical Biology, Jessenius Faculty of Medicine, Comenius University Bratislava, Slovak Republic.
authors.forename: Peter
authors.initials: P
authors.lastname: Kubatka
authors.affiliation: Department of Traumatology, Faculty of Medicine, Pavol Jozef Šafárik University and Louis Pasteur University Hospital Rastislavova 43, Košice, Slovak Republic.
authors.forename: Stanislava
authors.initials: S
authors.lastname: Pingorova
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Pavol Jozef Šafárik University Šrobárova 2, Košice, Slovak Republic.
authors.forename: Jarmila
authors.initials: J
authors.lastname: Vesela
mesh_terms: D000063:Accidents, Traffic; D056809:Alveolar Epithelial Cells / Q000276:immunology / Q000378:metabolism; D015703:Antigens, CD / Q000096:biosynthesis*; D015214:Antigens, Differentiation, Myelomonocytic / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D016676:Macrophages, Alveolar / Q000276:immunology / Q000378:metabolism; D008297:Male; D009000:Monocytes / Q000276:immunology / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism; D015541:Off-Road Motor Vehicles; D009195:Peroxidase / Q000096:biosynthesis*; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D012128:Respiratory Distress Syndrome / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis*; D055815:Young Adult; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D011956:Receptors, Cell Surface; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D009195:Peroxidase
keywords: ARDS; CD163; VEGF; immunohistochemistry; myeloperoxidase