title: Chemical composition, anti-inflammatory activity and cytotoxic activity of the liposoluble constituents from different parts of Acanthopanax evodiaefolius by the Herbal Blitzkrieg Extractor.
abstract: The liposoluble constituents of leaves (LCL) and stem barks (LCSB) from Acanthopanax evodiaefolius Franch were extracted by Herbal Blitzkrieg Extractor (HBE), and their chemical composition was analyzed by GC-MS. 18 compounds were identified, representing 71.98% of LCL, while 35 compounds were identified, accounting for 98.28% of the LCSB. Their cytotoxic activity and inhibitory effect on nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells were tested and the LCL showed significant NO, IL-6 and TNF-α inhibition activity. These results provide the scientific basis for looking for new natural anti-inflammatory substances and exploiting and developing resources of A. evodiaefolius Franch.
authors.affiliation: School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.
authors.forename: Wei-Chao
authors.initials: WC
authors.lastname: Huang
authors.affiliation: School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.affiliation: School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Li
authors.affiliation: School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Gong
authors.affiliation: School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. Electronic address: lxq0001cn@163.com.
authors.forename: Xiang-Qian
authors.initials: XQ
authors.lastname: Liu
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Chungbuk 363-883, Republic of Korea.
authors.forename: Ok-Kyoung
authors.initials: OK
authors.lastname: Kwon
authors.affiliation: School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.
authors.forename: Hui-Xuan
authors.initials: HX
authors.lastname: Ye
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Chungbuk 363-883, Republic of Korea.
authors.forename: Hyeong-Kyu
authors.initials: HK
authors.lastname: Lee
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D028001:Eleutherococcus / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide
keywords: Acanthopanax evodiaefolius; Anti-inflammatory; Cytotoxic activity; Herbal Blitzkrieg Extractor; Liposoluble constituents; Nitric oxide