title: Blood lipids affect rat islet blood flow regulation through β₃-adrenoceptors.
abstract: Pancreatic islet blood perfusion varies according to the needs for insulin secretion. We examined the effects of blood lipids on pancreatic islet blood flow in anesthetized rats. Acute administration of Intralipid to anesthetized rats increased both triglycerides and free fatty acids, associated with a simultaneous increase in total pancreatic and islet blood flow. A preceding abdominal vagotomy markedly potentiated this and led acutely to a 10-fold increase in islet blood flow associated with a similar increase in serum insulin concentrations. The islet blood flow and serum insulin response could be largely prevented by pretreatment with propranolol and the selective β₃-adrenergic inhibitor SR-59230A. The nitric oxide synthase inhibitor N(G)-nitro-l-arginine methyl ester prevented the blood flow increase but was less effective in reducing serum insulin. Increased islet blood flow after Intralipid administration was also seen in islet and whole pancreas transplanted rats, i.e., models with different degrees of chronic islet denervation, but the effect was not as pronounced. In isolated vascularly perfused single islets Intralipid dilated islet arterioles, but this was not affected by SR-59230A. Both the sympathetic and parasympathetic nervous system are important for the coordination of islet blood flow and insulin release during hyperlipidemia, with a previously unknown role for β₃-adrenoceptors.
authors.affiliation: Department of Physiology, Zhejiang University School of Medicine, Hangzhou, China;
authors.forename: Enyin
authors.initials: E
authors.lastname: Lai
authors.affiliation: Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden;
authors.forename: Ulrika
authors.initials: U
authors.lastname: Pettersson
authors.affiliation: Department of Surgical Sciences, Uppsala University, Uppsala, Sweden; and.
authors.forename: Alberto Delgado
authors.initials: AD
authors.lastname: Verdugo
authors.affiliation: Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden; Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
authors.forename: Per-Ola
authors.initials: PO
authors.lastname: Carlsson
authors.affiliation: Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden;
authors.forename: Birgitta
authors.initials: B
authors.lastname: Bodin
authors.affiliation: Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden;
authors.forename: Örjan
authors.initials: Ö
authors.lastname: Källskog
authors.affiliation: Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden;
authors.forename: A Erik G
authors.initials: AE
authors.lastname: Persson
authors.affiliation: Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden;
authors.forename: Monica
authors.initials: M
authors.lastname: Sandberg
authors.affiliation: Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden; Leif.Jansson@mcb.uu.se.
authors.forename: Leif
authors.initials: L
authors.lastname: Jansson
mesh_terms: D058673:Adrenergic beta-3 Receptor Antagonists / Q000494:pharmacology; D000818:Animals; D004655:Emulsions / Q000009:adverse effects; D005230:Fatty Acids, Nonesterified / Q000097:blood / Q000378:metabolism; D006949:Hyperlipidemias / Q000097:blood / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology*; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000098:blood supply* / Q000187:drug effects / Q000294:innervation / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D010179:Pancreas / Q000098:blood supply / Q000187:drug effects / Q000294:innervation / Q000378:metabolism; D010477:Perfusion; D010743:Phospholipids / Q000009:adverse effects; D011412:Propanolamines / Q000494:pharmacology; D011920:Rats, Inbred WF; D022702:Receptors, Adrenergic, beta-3 / Q000737:chemistry / Q000378:metabolism*; D012039:Regional Blood Flow / Q000187:drug effects; D013024:Soybean Oil / Q000009:adverse effects; D014280:Triglycerides / Q000097:blood / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D015167:Vagotomy, Truncal; D014630:Vagus Nerve / Q000187:drug effects / Q000503:physiopathology* / Q000601:surgery
chemical_list: C097869:3-(2-ethylphenoxy)-1-(1,2,3,4-tetrahydronaphth-1-ylamino)-2-propanol oxalate; D058673:Adrenergic beta-3 Receptor Antagonists; D004655:Emulsions; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D010743:Phospholipids; D011412:Propanolamines; D022702:Receptors, Adrenergic, beta-3; D014280:Triglycerides; C545823:soybean oil, phospholipid emulsion; D013024:Soybean Oil
keywords: pancreatic islets