title: Microarray analysis of intestinal immune-related gene expression in heat-stressed rats.
abstract: OBJECTIVE
  This study aimed to investigate immune-related gene expression in rat small intestine after heat stress.
  
  
  METHODS
  Twelve Sprague Dawley (SD) rats were randomly divided into control and heat-stressed groups. Rats in both groups were housed at 25 °C with 60% relative humidity. The heat-stressed group was subjected to 40 °C for 2 h/day for 3 days. After heat stress, the mRNA expression profile of small intestine epithelial tissue was evaluated by microarray analysis.
  
  
  RESULTS
  A total of 23 genes related to immune responses were significantly altered, of which 12 genes were up-regulated and 11 genes were down-regulated.
  
  
  CONCLUSIONS
  Microarray analysis demonstrated the JAK-STAT pathway had a potentially important role in the regulation of inflammation in the small intestine, and changes in antigen presentation might reduce intestinal immune responses after heat stress.
authors.affiliation: China Agricultural University-Beijing University of Agriculture Traditional Chinese Veterinary Medicine (CAU-BUA TCVM) Teaching and Research Team, College of Animal Science and Technology, Beijing University of Agriculture , Beijing .
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Liu
authors.forename: Yaran
authors.initials: Y
authors.lastname: Shi
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Hou
authors.forename: Changrong
authors.initials: C
authors.lastname: Wan
authors.forename: Shasha
authors.initials: S
authors.lastname: He
authors.forename: Xinlu
authors.initials: X
authors.lastname: Chong
authors.forename: Mingjiang
authors.initials: M
authors.lastname: Liu
authors.forename: Huanrong
authors.initials: H
authors.lastname: Li
authors.forename: Fenghua
authors.initials: F
authors.lastname: Liu
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D018869:Heat-Shock Response / Q000235:genetics* / Q000276:immunology*; D007421:Intestine, Small / Q000276:immunology* / Q000378:metabolism; D053612:Janus Kinases / Q000235:genetics; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D050791:STAT Transcription Factors / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D050791:STAT Transcription Factors; D053612:Janus Kinases
keywords: Heat stress; immune; microarray analysis; rat; small intestine