title: Usefulness of PCR-RFLP coa gene for clonal classification of methicillin-resistant Staphylococcus aureus isolates in tertiary hospitals.
abstract: One hundred and one methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates were classified into 10 genotypes based on their polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) coa pattern. PCR-RFLP coa patterns correlated with the clonal complex (CC) with the exception of CC5, which was related to 2 patterns (B and E). The PCR-RFLP coa gene technique provides a useful preliminary method to monitor variations in MRSA populations.
authors.affiliation: From the Servicio de Microbiología, Hospital Clínico Universitario Lozano Blesa , Zaragoza.
authors.forename: María
authors.initials: M
authors.lastname: González-Domínguez
authors.forename: Carmen
authors.initials: C
authors.lastname: Potel
authors.forename: Cristina
authors.initials: C
authors.lastname: Seral
authors.forename: Lucía
authors.initials: L
authors.lastname: Constenla
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Castillo
authors.forename: Maximiliano
authors.initials: M
authors.lastname: Alvarez
mesh_terms: D003030:Coagulase / Q000235:genetics*; D005838:Genotype; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D062606:Tertiary Care Centers
chemical_list: D003030:Coagulase
keywords: MRSA; PCR-RFLP; coa gene; spa typing