title: Boronate affinity nanoparticles for nucleoside separation.
abstract: Boronate affinity systems have been recently used for the specific isolation of cis-diol group carrying biomolecules such as glycoproteins, nucleosides, carbohydrates. Nanosized materials have been extremely used for the biotechnological purposes due to their unique properties and their high surface areas. The objective of this presented work was to develop a new boronate affinity system for the nucleoside adsorption. For this purpose, poly(HEMA) nanoparticles were synthesized by using surfactant free emulsion polymerization technique and then functionalized with phenylboronic acid. Synthesized nanoparticles were characterized with FTIR, SEM, and Zeta size analysis. Nucleic acid adsorption experiments were repeated for different medium pH values, for various nucleosides concentrations, for different temperatures and ionic strengths, in order to determine the optimum adsorption conditions. In the light of these studies, it can be concluded that this boronate ligand carrying nanoparticles were very valuable for the separation of nucleosides.
authors.affiliation: a Department of Biochemistry , Ege University , İzmir , Turkey.
authors.forename: Burcu
authors.initials: B
authors.lastname: Okutucu
authors.affiliation: a Department of Biochemistry , Ege University , İzmir , Turkey.
authors.forename: Deniz
authors.initials: D
authors.lastname: Vurmaz
authors.affiliation: a Department of Biochemistry , Ege University , İzmir , Turkey.
authors.forename: Akile
authors.initials: A
authors.lastname: Tuncal
authors.affiliation: a Department of Biochemistry , Ege University , İzmir , Turkey.
authors.forename: Ceren
authors.initials: C
authors.lastname: Türkcan
authors.affiliation: b Department of Chemistry , Adnan Menderes University , Aydın , Turkey.
authors.forename: Deniz
authors.initials: D
authors.lastname: Aktaş Uygun
authors.affiliation: a Department of Biochemistry , Ege University , İzmir , Turkey.
authors.forename: Sinan
authors.initials: S
authors.lastname: Akgöl
mesh_terms: D000327:Adsorption; D001897:Boronic Acids / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D004655:Emulsions; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009705:Nucleosides / Q000302:isolation & purification*; D009994:Osmolar Concentration; D011073:Polyamines / Q000737:chemistry*; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives* / Q000737:chemistry; D058105:Polymerization; D013696:Temperature
chemical_list: D001897:Boronic Acids; D004655:Emulsions; D009705:Nucleosides; D011073:Polyamines; C056971:poly(2-hydroxyethyl methacrylate)-polyamine graft copolymer; D011102:Polyhydroxyethyl Methacrylate; C010686:benzeneboronic acid
keywords: boronate affinity; nanoparticle; nucleoside; phenylboronic acid