title: Regulatory effect of iron regulatory protein-2 on iron metabolism in lung cancer.
abstract: Iron metabolism plays an important role in the pathogenesis of lung cancer. This study aimed to investigate the effect of gene silencing of iron regulatory protein-2 (IRP2) on mRNA and protein expression of transferrin (Tf), transferrin receptor (TfR), and ferritin (Fn) in A549 lung cancer cells. A549 cells were cultured and divided into a liposome control group, a liposome + oligonucleotide (SCODN) control group, and a Lipofectamine + antisense oligonucleotide (ASODN) group. RT-PCR and Western blotting were used to detect mRNA and protein expression of Tf, TfR, and Fn. We found no significant change in Tf mRNA expression among the 3 groups (P = 0.078). TfR and Fn mRNA expressions in the ASODN group notably decreased compared to the liposome and SCODN groups (P < 0.01). IRP2 and TfR protein expressions in the ASODN group were significantly lower than in the liposome or SCODN groups (P < 0.05), whereas no significant change in Tf protein expression was observed between the 3 groups (P = 0.088). Fn protein expression in the ASODN group was significantly higher than in the liposome or SCODN group (P < 0.05). IRP2 can regulate the expression of TfR and Fn by changing its own protein expression and thereby regulate iron metabolism.
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Institute of Clinical Medicine, Zhengzhou, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Institute of Clinical Medicine, Zhengzhou, China.
authors.forename: L L
authors.initials: LL
authors.lastname: Dai
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Institute of Clinical Medicine, Zhengzhou, China.
authors.forename: Y N
authors.initials: YN
authors.lastname: Song
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Institute of Clinical Medicine, Zhengzhou, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Kang
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Institute of Clinical Medicine, Zhengzhou, China.
authors.forename: J M
authors.initials: JM
authors.lastname: Si
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Institute of Clinical Medicine, Zhengzhou, China.
authors.forename: J
authors.initials: J
authors.lastname: Xia
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Institute of Clinical Medicine, Zhengzhou, China yufengliudoc@163.com.
authors.forename: Y F
authors.initials: YF
authors.lastname: Liu
mesh_terms: D045744:Cell Line, Tumor; D005293:Ferritins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007501:Iron / Q000378:metabolism*; D035942:Iron Regulatory Protein 2 / Q000235:genetics* / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011990:Receptors, Transferrin / Q000235:genetics; D014162:Transfection; D014168:Transferrin / Q000235:genetics / Q000378:metabolism
chemical_list: D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D011990:Receptors, Transferrin; D014168:Transferrin; D005293:Ferritins; D007501:Iron; D035942:Iron Regulatory Protein 2