title: Effects of parathyroid hormone on calcium ions in rat bone marrow mesenchymal stem cells.
abstract: The present study was conducted in order to explore the mechanisms whereby parathyroid hormone (PTH) maintains in vitro proliferation of bone marrow mesenchymal stem cells (BMSCs). Bone marrow was isolated from Sprague Dawley (SD) rat femurs, cultured in vitro, and passaged using a cell adherent culture method. The BMSC proliferation was evaluated by the methyl thiazolyl tetrazolium (MTT) assay and the fluorescence intensity of calcium ions in BMSCs was analyzed by laser scanning confocal microscopy (LSCM). Our results show that BMSC proliferation in the experimental group treated with PTH was more significant than controls. The calcium ion fluorescence intensity in BMSCs was significantly higher for the experimental group as compared to the control group. For each group, there was significant difference in the fluorescence intensity of calcium ions in BMSCs between 7 d and 14 d. In conclusion, parathyroid hormone increased the fluorescence intensity of calcium ions in BMSCs, which might represent a key mechanism whereby BMSC proliferation is maintained.
authors.affiliation: Department of Orthopaedic Surgery, Orthopedics Implantation Key Lab of Guangdong Province, 1st Affiliated Hospital of Guangzhou Medical University, No. 151 Yanjiang Road, Guangzhou 510120, China.
authors.forename: Yushu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Orthopaedic Surgery, Orthopedics Implantation Key Lab of Guangdong Province, 1st Affiliated Hospital of Guangzhou Medical University, No. 151 Yanjiang Road, Guangzhou 510120, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Bai
authors.affiliation: Department of Orthopaedic Surgery, Orthopedics Implantation Key Lab of Guangdong Province, 1st Affiliated Hospital of Guangzhou Medical University, No. 151 Yanjiang Road, Guangzhou 510120, China.
authors.forename: Shujiang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedic Surgery, 1st People Hospital of Yunfu, No. 100 Construction of North Road, Yunfu 527300, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ye
authors.affiliation: Department of Orthopaedic Surgery, Orthopedics Implantation Key Lab of Guangdong Province, 1st Affiliated Hospital of Guangzhou Medical University, No. 151 Yanjiang Road, Guangzhou 510120, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Biomechanics & Medical Information Institute, Beijing University of Technology, No. 100 Flat Land Chaoyang District, Beijing 100022, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D005269:Femur / Q000166:cytology / Q000378:metabolism*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010281:Parathyroid Hormone; D002118:Calcium