title: Functional assay using lectin gene targeting technologies (over-expression).
abstract: Function of lectin depends on its amino acid sequence of carbohydrate-recognition domain (CRD), conformation, and extracellular/intracellular localization. Altering lectin gene expression by over-expression or knockdown is a powerful tool for analyzing its cellular function. Here, we describe a method of lectin gene over-expression, taking a C-type lectin, mannan-binding protein (MBP), as an example. Carbohydrate-binding ability of MBP, its subcellular localization, and functional co-localization with ligand glycoprotein are assayed comparing with an inactive mutant MBP.
authors.affiliation: Glycobiology Unit, Sanford-Burnham Medical Research Institute, 10901 North Torrey Pines Road, La Jolla, CA, 92037, USA, mnonaka@sanfordburnham.org.
authors.forename: Motohiro
authors.initials: M
authors.lastname: Nonaka
authors.forename: Toshisuke
authors.initials: T
authors.lastname: Kawasaki
mesh_terms: D045744:Cell Line, Tumor; D015870:Gene Expression; D018390:Gene Targeting / Q000379:methods*; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D037102:Lectins / Q000235:genetics* / Q000378:metabolism*; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism; D008351:Mannans / Q000378:metabolism; D037601:Mannose-Binding Lectin / Q000235:genetics / Q000378:metabolism; D008863:Microspheres; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D021381:Protein Transport; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D015699:HIV Envelope Protein gp120; D037102:Lectins; D037181:Lectins, C-Type; D008351:Mannans; D037601:Mannose-Binding Lectin; D011994:Recombinant Proteins