title: [Genome amplification and sequence analysis of human bocavirus 2].
abstract: To obtain the genome sequence of human bocavirus 2 (HBoV2), different regions of HBoV2 genome were amplified through PCR in fecal specimens which had been identified as single-positive for HBoV2 in 2010. A genome sequence of HBoV2 (HBoV2-NC, 5444 bp) was obtained after sequence assembly. The phylogenetic analysis showed that HBoV2-NC had the closest evolutionary relationship with HBoV2 Lanzhou strain. The predication of inverted terminal repeats of HBoV2-NC by DINAMelt showed that inverted terminal repeats were contained in HBoV2-NC 5' terminal, which had the typical stem-loop structure in other parvoviruses. Finally, some flanking sequences of HBoV2-NC were amplified by linker-PCR.
authors.forename: Huan-Di
authors.initials: HD
authors.lastname: Cui
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
authors.forename: Guang-Cheng
authors.initials: GC
authors.lastname: Xie
authors.forename: Wei-Xia
authors.initials: WX
authors.lastname: Cheng
authors.forename: Zhao-Jun
authors.initials: ZJ
authors.lastname: Duan
mesh_terms: D001483:Base Sequence; D005784:Gene Amplification; D016679:Genome, Viral; D056661:Human bocavirus / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010322:Parvoviridae Infections / Q000821:virology*; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020079:Terminal Repeat Sequences
chemical_list: D012367:RNA, Viral