title: Icotinib inhibits the invasion of Tca8113 cells via downregulation of nuclear factor κB-mediated matrix metalloproteinase expression.
abstract: Icotinib is an epidermal growth factor receptor tyrosine kinase inhibitor, which has been revealed to inhibit proliferation in tumor cells. However, the effect of icotinib on cancer cell metastasis remains to be explained. This study examines the effect of icotinib on the migration and invasion of squamous cells of tongue carcinoma (Tca8113 cells)in vitro. The results of the Boyden chamber invasion assay demonstrated that icotinib reduced cell invasion, suppressed the protein levels of matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and increased the expression of tissue inhibitor of metalloproteinase-1. In addition, icotinib was found to significantly decrease the protein levels of nuclear factor κB (NF-κB) p65, which suggested that icotinib inhibits NF-κB activity. Furthermore, treatment with the NF-κB inhibitor, pyrrolidine dithiocarbamate, suppressed cell invasion and MMP-2 expression. These results suggested that icotinib inhibits the invasion of Tca8113 cells by downregulating MMP via the inactivation of the NF-κB signaling pathways.
authors.affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Xinxiang Medical University, Weihui, Henan 453100, P.R. China.
authors.forename: Cailing
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Human Anatomy, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Guoyan
authors.initials: G
authors.lastname: Yuan
authors.affiliation: Department of Human Anatomy, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Internal Digestive Medicine, The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Derong
authors.initials: D
authors.lastname: Lu
authors.affiliation: Department of Human Anatomy, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Mingxin
authors.initials: M
authors.lastname: Ren
authors.affiliation: Department of Human Anatomy, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
authors.forename: Weigang
authors.initials: W
authors.lastname: Cui
keywords: Tca8113 cells; icotinib; matrix metallopeptidase; nuclear factor κB; tumor invasion