title: Evaluation of the oxidative status in Oreochromis niloticus fed with tuna by-product meal: possible human health impact.
abstract: The influence of tuna by-product meal (TBM) diets on the activities of antioxidant enzymes in the muscles, gill and kidney of tilapia was evaluated. The control diet (A0) used fish meal (FM) as the sole source of animal protein and the other three diets (A10-A30), 33%-100% of FM was substituted by TBM at 10% increments. The cytotoxicity of liver and muscles cytosol extracts in human liver cell HepG2 was undertaken. The activities of glutathione S-transferase, superoxide dismutase, catalase and TBARS values in the kidney of fish fed with diet A30 were significantly different (p<0.05) and no alterations were observed in muscle and gill anti-oxidant activities. Our study has confirmed that the cytosol extract does not exhibit cytotoxic effects on the HepG2 cells. These results indicate that the use of TBM as a total replacement for FM into tilapia diets seems to have oxidative stress, thus generating effect on fish metabolism.
authors.affiliation: Laboratory of Animal Ecophysiology/Department of Life Sciences , Sfax , Tunisia .
authors.forename: Saber Abdelkader
authors.initials: SA
authors.lastname: Saїdi
authors.forename: Mohamed Salah
authors.initials: MS
authors.lastname: Azaza
authors.forename: Jos
authors.initials: J
authors.lastname: van Pelt
authors.forename: Abdelfattah
authors.initials: A
authors.lastname: El Feki
mesh_terms: D000821:Animal Feed / Q000633:toxicity*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D023681:Cichlids / Q000254:growth & development / Q000378:metabolism*; D004032:Diet / Q000662:veterinary*; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism; D014413:Tuna
chemical_list: D000975:Antioxidants; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Cell toxicity; Oreochromis niloticus; oxidative stress; tuna by-product meal