title: Influence of As2O3 combined with ginsenosides Rg3 on inhibition of lung cancer NCI-H1299 cells and on subsistence of nude mice bearing hepatoma.
abstract: OBJECTIVE
  To study the effect of arsenic trioxide (As2O3) combined with ginsenosides Rg3 on inhibiting the NCI-H1299 lung cancer cells and subsistence in nude mice bearing hepatoma.
  
  
  METHODS
  MTT method was used to measure the inhibition effect of As2O3 combined Rg3 on NCI-H1299 cells, and the proliferation inhibiting effect was observed via establishing the transplanted tumor model in vitro. A total of 40 tumor-bearing nude mice were randomly divided into normal saline group, As2O3, Rg3 and As2O3+Rg3 group. Transplantation tumor model of lung cancer in nude mice was constructed, followed by injection of certain concentrations of normal saline, As2O3, ginseng saponin Rg3 and As2O3+Rg3 every day. The survival duration and the tumors size of the mice were recorded and the Kaplan-Meier curve was made; microscopic observation of apoptosis of tumor cells in vivo was done using TUNEL staining.
  
  
  RESULTS
  After 72 h of injection, inhibition rate of tumor cell in normal saline group, As2O3 group, Rg3 group and As2O3+Rg3 group was (5.66±0.31)%, (65.58±4.75)%, (44.69±3.32)% and (82.67±5.43)%, respectively. Inhibition rate of tumor cell in As2O3 group, Rg3 group and As2O3+Rg3 group was significantly higher than that of normal saline group (P<0.01); inhibition rate of tumor cells of As2O3+Rg3 group was significantly higher than that of the two groups given As2O3 or Rg3 alone (P<0.01). The tumor volume of As2O3 group, Rg3 group and As2O3+Rg3 group shrank to (65.38±3.25)%, (77.68±3.43)% and (42.65±3.55)% of the original, tumor volume of saline group was 1.21 times of the original size (P<0.01); Median survival of saline group, Rg3 group, As2O3 group were significantly shorter than that of As2O3+Rg3 group (P<0.01); co-ordinated intervention ability of As2O3+Rg3 on NCI-H1299 cell was significantly higher than that of As2O3 or Rg3, separately.
  
  
  CONCLUSIONS
  As2O3 combined with Rg3 can significantly inhibit proliferation of NCI-H1299 cells in lung cancer, prolong survival of tumor-bearing nude mice, and promote tumor cell apoptosis, and have significant effect on lung cancer treatment.
authors.affiliation: Huaihe River Hospital, Henan University, Kaifeng 475000, Henan, China.
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Che
authors.affiliation: Huaihe River Hospital, Henan University, Kaifeng 475000, Henan, China.
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Liu
authors.affiliation: Huaihe River Hospital, Henan University, Kaifeng 475000, Henan, China.
authors.forename: Hong-Bing
authors.initials: HB
authors.lastname: Ma
authors.affiliation: Huaihe River Hospital, Henan University, Kaifeng 475000, Henan, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Huaihe River Hospital, Henan University, Kaifeng 475000, Henan, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Huaihe River Hospital, Henan University, Kaifeng 475000, Henan, China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Li
authors.affiliation: Huaihe River Hospital, Henan University, Kaifeng 475000, Henan, China.
authors.forename: Wei-Chao
authors.initials: WC
authors.lastname: Liu
authors.affiliation: Huaihe River Hospital, Henan University, Kaifeng 475000, Henan, China. Electronic address: chejbt@163.com.
authors.forename: Gong-Ning
authors.initials: GN
authors.lastname: Shi
keywords: As(2)O(3); Combination therapy; Lung cancer; Rg3