title: Accuracy of fine needle aspiration biopsy processed by cytologic smear and cell block techniques for the diagnosis of lacrimal gland tumors: a study of 48 cases.
abstract: OBJECTIVE
  To study the accuracy of fine needle aspiration biopsy (FNAB) processed by smear cytology and cell block (CB) techniques for the diagnosis of lacrimal gland tumors (LGTs).
  
  
  METHODS
  In a prospective study, we enrolled 48 consecutive patients with LGTs. Immediately after excision of LGTs, the tissues were underwent FNAB with 23-gauge needles. The FNAB samples were processed to produce cytologic smears and CB from which slides were cut for immunohistochemical staining. The remainders were submitted for routine histopathologic processing. The diagnostic value of FNAB was assessed by comparing the FNAB diagnoses to those made by routine histopathology.
  
  
  RESULTS
  Cytopathologic evaluations based on smear cytology and CB with sections stained immunohistochemically can distinguish non-epithelial lesions from epithelial ones in all cases. The diagnostic sensitivities, specificities, and accuracies for distinguishing benign from malignant lesions were: cytologic smears--76%, 68%, and 71%, respectively; CB with immunohistochemical staining--88%, 87%, and 88%, respectively. The accuracy of the tissue diagnosis compared to routine histopathology was less for cytologic smears (58%) than for CB with immunohistochemistry (81%; P < 0.05).
  
  
  CONCLUSIONS
  FNAB of LGT processed using a CB technique capable of producing immunohistochemically stained slides results in a greater percentage of accurate tissue diagnoses than do cytologic smears, when compared to routine histopathology.
authors.affiliation: Department of Ophthalmology, Eye and ENT Hosoital of Fudan University Shanghai, China.
authors.forename: Xiangning
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, Eye and ENT Hosoital of Fudan University Shanghai, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Qian
authors.affiliation: Department of Ophthalmology, Eye and ENT Hosoital of Fudan University Shanghai, China.
authors.forename: Yifei
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Pathology, Eye and ENT Hosoital of Fudan University Shanghai, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Ping
authors.affiliation: Department of Pathology, Eye and ENT Hosoital of Fudan University Shanghai, China.
authors.forename: Liqing
authors.initials: L
authors.lastname: Feng
authors.affiliation: Department of Pathology, Fudan University Shanghai Cancer Center China.
authors.forename: Yingwen
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Department of Pathology, Fudan University Shanghai Cancer Center China.
authors.forename: Xiaping
authors.initials: X
authors.lastname: Li
mesh_terms: D044963:Biopsy, Fine-Needle; D003581:Cytodiagnosis / Q000379:methods*; D005134:Eye Neoplasms / Q000175:diagnosis*; D006801:Humans; D007150:Immunohistochemistry; D007766:Lacrimal Apparatus Diseases / Q000175:diagnosis*; D012680:Sensitivity and Specificity
keywords: Lacrimal gland lesions; cell blocks; cytopathology; fine needle aspiration; immnohistichemistry