title: Assessment of IgG antibodies to Pseudomonas aeruginosa in patients with cystic fibrosis by an enzyme-linked immunosorbent assay (ELISA).
abstract: BACKGROUND
  The usefulness of serological tests for detection of P. aeruginosa pulmonary infection in cystic fibrosis (CF) is controversial. Here, we assessed the value of detecting anti-P. aeruginosa IgG by a quantitative enzyme-linked immunosorbent assay (ELISA) for identification of P. aeruginosa infection in patients with cystic fibrosis.
  
  
  METHODS
  Serum concentrations of anti-P. aeruginosa IgG were assessed in 117 CF patients classified according to their P. aeruginosa colonization/infection status (never colonized; free of infection; intermittently colonized and chronically infected) and in 53 healthy subjects by the ELISA test standardized with the St-Ag:1-17 antigen.
  
  
  RESULTS
  The rate of IgG seropositivity and the median of IgG concentrations of this antibody in patients chronically infected were significantly higher than those found in the other CF groups and in the healthy control group.
  
  
  CONCLUSIONS
  Detection of anti-P. aeruginosa IgG can be an useful tool for identification of P. aeruginosa chronic infection in patients with CF.
  The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_158.
authors.forename: Renan Marrichi
authors.initials: RM
authors.lastname: Mauch
authors.forename: Cláudio Lúcio
authors.initials: CL
authors.lastname: Rossi
authors.forename: José Dirceu
authors.initials: JD
authors.lastname: Ribeiro
authors.forename: Antônio Fernando
authors.initials: AF
authors.lastname: Ribeiro
authors.forename: Marcos Tadeu
authors.initials: MT
authors.lastname: Nolasco da Silva
authors.forename: Carlos Emílio
authors.initials: CE
authors.lastname: Levy
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000150:complications / Q000276:immunology* / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007223:Infant; D008297:Male; D011552:Pseudomonas Infections / Q000150:complications / Q000175:diagnosis* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000276:immunology* / Q000302:isolation & purification; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G