title: [Analytical performances of SPAPLUS® turbidimeter for the dosage of immunoglobulins and beta-2 microglobulin in serum].
abstract: We evaluated the analytical performances of the SPAPLUS(®) immunoturbidimeter assays manufactured by The Binding Site(®) for the quantification of thirteen immunological parameters in serum: IgG, IgA, IgM and IgD immunoglobulins, IgG subclasses (1 to 4), IgA subclasses (1 and 2), beta-2 microglobulin, free light chains kappa and lambda. The within-day precision (repeatability) and the between-day precision (reproducibility) were obtained for two or three concentration levels depending of the parameter and were below the recommendations of the manufacturer, except for the repeatability of IgG1 (at a level of concentration of 6.7 g/L). An agreement above 90% (with Bland and Altman analysis) was observed between the results obtained with SPAPLUS(®) and those obtained with the nephelometer IMMAGE(®) 800 or radial immunodiffusion. The evaluation confirmed the linearity of the assays and the absence of contamination for all the parameters tested. We also assessed the practicability of the SPAPLUS(®) in terms of maintenance, frequency of calibration and cadence tests. The SPAPLUS(®) immunoturbidimeter displays good analytical performances for the immunological parameters evaluated in the present work.
authors.affiliation: Laboratoire d'immunologie, CHU de Toulouse, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Thevenet
authors.affiliation: Laboratoire d'immunologie, CHU de Toulouse, France.
authors.forename: Clarisse
authors.initials: C
authors.lastname: Benat
authors.affiliation: Laboratoire d'immunologie, CHU de Toulouse, France.
authors.forename: Jasmine
authors.initials: J
authors.lastname: Chauzeix
authors.affiliation: Laboratoire d'immunologie, CHU de Toulouse, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Blancher
authors.affiliation: Laboratoire d'immunologie, CHU de Toulouse, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Puissant-Lubrano
mesh_terms: D006801:Humans; D007136:Immunoglobulins / Q000097:blood*; D007159:Immunologic Tests / Q000295:instrumentation*; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation*; D015203:Reproducibility of Results; D001613:beta 2-Microglobulin / Q000097:blood*
chemical_list: D007136:Immunoglobulins; D001613:beta 2-Microglobulin
keywords: analytical performances; immunoglobulins; immunology; turbidimetry