title: May the thyroid gland and thyroperoxidase participate in nitrosylation of serum proteins and sporadic Parkinson's disease?
abstract: The research group has detected nitrosative stress and a singular version of nitrosylated serum α-synuclein in serum of Parkinson's disease (PD) patients. Dysfunction of the thyroid gland has been proposed to be linked to this disease. The aim of the study was to know if the thyroid gland is involved in idiopathic PD and nitrosative stress. We studied 50 patients (early and advanced disease patients), 35 controls, and 6 subjects with thyroidectomy. Clinical characteristics, serum thyroperoxidase levels, and 3-nitrotyrosine proteins were analyzed. Enzyme-linked immunosorbent assay and immunoblotting methods were employed. The findings indicated that the prevalence of two thyroid dysfunctions (hyper- or hypothyroidism) was not found to be different in patients relative to controls. However, the levels of the enzyme thyroperoxidase were found to be elevated in early disease patients (p<0.006), not in advanced disease subjects, and these levels were negatively correlated with serum 3-nitrotyrosine proteins (p<0.05), the indicators of nitrosative stress. The thyroidectomized subjects showed very low levels of serum 3-nitrotyrosine proteins (78% reduction vs. controls) and, among these proteins, the nitrosylated serum α-synuclein was nearly absent. These observations lead to the hypothesis that the thyroid gland and thyroperoxidase participate in nitrosylation of serum proteins and they could influence Parkinsonian nitrosative stress as well as nitrosylation of serum α-synuclein, a potentially pathogenic factor.
authors.affiliation: 1 Laboratory of Neurophysiology and Molecular Neurology (BIO127), Department of Medical Physiology and Biophysics, Faculty of Medicine, University of Seville , Seville, Spain .
authors.forename: Emilio
authors.initials: E
authors.lastname: Fernández
authors.forename: José-Manuel
authors.initials: JM
authors.lastname: García-Moreno
authors.forename: Angel
authors.initials: A
authors.lastname: Martín de Pablos
authors.forename: José
authors.initials: J
authors.lastname: Chacón
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007453:Iodide Peroxidase; D008297:Male; D008875:Middle Aged; D010300:Parkinson Disease / Q000097:blood* / Q000201:enzymology; D011499:Protein Processing, Post-Translational; D013961:Thyroid Gland / Q000201:enzymology*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: C002744:3-nitrotyrosine; D014443:Tyrosine; D007453:Iodide Peroxidase