title: Transcriptional responses of the bacterial antagonist Serratia plymuthica to the fungal phytopathogen Rhizoctonia solani.
abstract: Rhizobacteria with biocontrol ability exploit a range of mechanisms to compete successfully with other microorganisms and to ensure their growth and survival in the rhizosphere, ultimately promoting plant growth. The rhizobacterium Serratia plymuthica AS13 is able to promote oilseed rape growth and improve seedling survival in the presence of the fungal pathogen, Rhizoctonia solani AG 2-1; however, our understanding of the mechanisms underlying the antagonism of Serratia is limited. To elucidate possible mechanisms, genome-wide gene expression profiling of S. plymuthica AS13 was carried out in the presence or absence of R. solani. We used RNA sequencing methodology to obtain a comprehensive overview of Serratia gene expression in response to R. solani. The differential gene expression profiles of S. plymuthica AS13 revealed significantly increased expression of genes related to the biosynthesis of the antibiotic pyrrolnitrin (prnABCD), protease production and transporters. The results presented here provide evidence that antibiosis is a major functional mechanism underlying the antagonistic behaviour of S. plymuthica AS13.
authors.affiliation: Uppsala BioCenter, Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Box 7026, Uppsala, SE-750 07, Sweden.
authors.forename: Saraswoti
authors.initials: S
authors.lastname: Neupane
authors.forename: Roger D
authors.initials: RD
authors.lastname: Finlay
authors.forename: Sadhna
authors.initials: S
authors.lastname: Alström
authors.forename: Malin
authors.initials: M
authors.lastname: Elfstrand
authors.forename: Nils
authors.initials: N
authors.lastname: Högberg
mesh_terms: D000898:Antibiosis; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D029741:Brassica rapa / Q000254:growth & development / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology*; D012232:Rhizoctonia / Q000502:physiology*; D012705:Serratia / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins