title: Molecular analysis of interleukin-25 exons 1 and 2 and its serum levels in Iranian patients with multiple sclerosis.
abstract: Overexpression of Interleukin-17 (IL-17) family has been shown in a variety of autoimmune diseases. IL-25 (IL-17E), as a member of this family of cytokines, induces the overexpression of IL-13 and impedes Th17/IL-17 responses. In the present study potential single nucleotide polymorphisms (SNP) of IL-25, its serum level in Multiple Sclerosis (MS) patients have been surveyed. Blood samples were obtained from 100 Relapsing-Remitting MS cases, and 100 healthy controls. Serum levels of IL-25 were measured by ELISA. IL-25 exons 1 and 2 were sequenced. IL-25 serum levels investigation showed significant association in cases compared to controls. Molecular analysis of IL-25exons 1 and 2 depicted significant differences in polymorphisms of exon 2 between two groups of study. However, no significant differences were found in polymorphisms for IL-25 exon. These results demonstrate that serum levels of IL-25 are reduced in MS patients compared to controls. This is the first study in Iran that shows polymorphisms in IL-25 among MS patients. Considering the role of IL-25 in suppression of the effects of IL-17A and active phase of Experimental Autoimmune Encephalomyelitis (EAE) in vivo, this cytokine seems to have therapeutic potentials for autoimmune diseases like MS.
authors.affiliation: Department of Biochemistry and Immunology, Faculty of Medicine, Zabol University of Medical Sciences Sistan and Baluchestan, Iran.
authors.forename: Mohammad-Reza
authors.initials: MR
authors.lastname: Javan
authors.affiliation: Department of Immunology, School of Medicine, Tabriz University of Medical Sciences Tabriz, Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Seyfizadeh
authors.affiliation: Department of Immunology, School of Medicine, Tehran University of Medical Sciences Tehran, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Aslani
authors.affiliation: Department of Neurology & Neuroscience, Neurosciences Research Center, Tabriz University of Medical Sciences Tabriz, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Farhoodi
authors.affiliation: Department of Immunology, School of Medicine, Tabriz University of Medical Sciences Tabriz, Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Babaloo
keywords: ELISA; IL-25; gene polymorphism; multiple sclerosis