title: Analysis of N-acylhomoserine lactone dynamics in continuous cultures of Pseudomonas putida IsoF by use of ELISA and UHPLC/qTOF-MS-derived measurements and mathematical models.
abstract: In this interdisciplinary approach, the dynamics of production and degradation of the quorum sensing signal 3-oxo-decanoylhomoserine lactone were studied for continuous cultures of Pseudomonas putida IsoF. The signal concentrations were quantified over time by use of monoclonal antibodies and ELISA. The results were verified by use of ultra-high-performance liquid chromatography. By use of a mathematical model we derived quantitative values for non-induced and induced signal production rate per cell. It is worthy of note that we found rather constant values for different rates of dilution in the chemostat, and the values seemed close to those reported for batch cultures. Thus, the quorum-sensing system in P. putida IsoF is remarkably stable under different environmental conditions. In all chemostat experiments, the signal concentration decreased strongly after a peak, because emerging lactonase activity led to a lower concentration under steady-state conditions. This lactonase activity probably is quorum sensing-regulated. The potential ecological implication of such unique regulation is discussed.
authors.affiliation: Research Unit Microbe-Plant Interactions, Helmholtz Zentrum München, German Research Centre for Environmental Health (GmbH), Ingolstädter Landstrasse 1, 85764, Neuherberg, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Buddrus-Schiemann
authors.forename: Martin
authors.initials: M
authors.lastname: Rieger
authors.forename: Marlene
authors.initials: M
authors.lastname: Mühlbauer
authors.forename: Maria Vittoria
authors.initials: MV
authors.lastname: Barbarossa
authors.forename: Christina
authors.initials: C
authors.lastname: Kuttler
authors.forename: Burkhard A
authors.initials: BA
authors.lastname: Hense
authors.forename: Michael
authors.initials: M
authors.lastname: Rothballer
authors.forename: Jenny
authors.initials: J
authors.lastname: Uhl
authors.forename: Juliano R
authors.initials: JR
authors.lastname: Fonseca
authors.forename: Philippe
authors.initials: P
authors.lastname: Schmitt-Kopplin
authors.forename: Michael
authors.initials: M
authors.lastname: Schmid
authors.forename: Anton
authors.initials: A
authors.lastname: Hartmann
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D008962:Models, Theoretical; D016958:Pseudomonas putida / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*
chemical_list: C088386:homoserine lactone; D015107:4-Butyrolactone