title: Phenotypic and genotypic changes over time and across facilities of serial colonizing and infecting Escherichia coli isolates recovered from injured service members.
abstract: Escherichia coli is the most common colonizing and infecting organism isolated from U.S. service members injured during deployment. Our objective was to evaluate the phenotypic and genotypic changes of infecting and colonizing E. coli organisms over time and across facilities to better understand their transmission patterns. E. coli isolates were collected via surveillance cultures and infection workups from U.S. military personnel injured during deployment (June 2009 to May 2011). The isolates underwent antimicrobial susceptibility testing, pulsed-field gel electrophoresis, and multiplex PCR for phylotyping to determine their resistance profiles and clonality. A total of 343 colonizing and 136 infecting E. coli isolates were analyzed, of which 197 (57%) and 109 (80%) isolates, respectively, produced extended-spectrum β-lactamases (ESBL). Phylogroup A was predominant among both colonizing (38%) and infecting isolates (43%). Although 188 unique pulsed-field types (PFTs) were identified from the colonizing isolates, and 54 PFTs were identified from the infecting isolates, there was a lack of PFT overlap between study years, combat zones, and military treatment facilities. On a per-subject basis, 26% and 32% of the patients with serial colonizing isolates and 10% and 21% with serial infecting isolates acquired changes in their phylogroup and PFT profiles, respectively, over time. The production of ESBL remained high over time and across facilities, with no substantial changes in antimicrobial susceptibilities. Overall, our results demonstrated an array of genotypic and phenotypic differences for the isolates without large clonal clusters; however, the same PFTs were occasionally observed in the colonizing and infecting isolates, suggesting that the source of infections may be endogenous host organisms.
authors.affiliation: San Antonio Military Medical Center, JBSA Fort Sam Houston, Texas, USA Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA katrin.mende.ctr@mail.mil.
authors.forename: Katrin
authors.initials: K
authors.lastname: Mende
authors.affiliation: San Antonio Military Medical Center, JBSA Fort Sam Houston, Texas, USA.
authors.forename: Miriam L
authors.initials: ML
authors.lastname: Beckius
authors.affiliation: San Antonio Military Medical Center, JBSA Fort Sam Houston, Texas, USA Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.
authors.forename: Wendy C
authors.initials: WC
authors.lastname: Zera
authors.affiliation: San Antonio Military Medical Center, JBSA Fort Sam Houston, Texas, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Yu
authors.affiliation: San Antonio Military Medical Center, JBSA Fort Sam Houston, Texas, USA.
authors.forename: Kristelle A
authors.initials: KA
authors.lastname: Cheatle
authors.affiliation: Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.
authors.forename: Deepak
authors.initials: D
authors.lastname: Aggarwal
authors.affiliation: Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: Landstuhl Regional Medical Center, Landstuhl, Germany.
authors.forename: Bradley A
authors.initials: BA
authors.lastname: Lloyd
authors.affiliation: Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.
authors.forename: David R
authors.initials: DR
authors.lastname: Tribble
authors.affiliation: Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA Walter Reed National Military Medical Center, Bethesda, Maryland, USA.
authors.forename: Amy C
authors.initials: AC
authors.lastname: Weintrob
authors.affiliation: San Antonio Military Medical Center, JBSA Fort Sam Houston, Texas, USA.
authors.forename: Clinton K
authors.initials: CK
authors.lastname: Murray
mesh_terms: D000328:Adult; D002353:Carrier State / Q000453:epidemiology / Q000382:microbiology*; D016000:Cluster Analysis; D015331:Cohort Studies; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008889:Military Personnel; D058889:Molecular Typing; D010641:Phenotype; D016133:Polymerase Chain Reaction; D013997:Time Factors; D055815:Young Adult