title: Enzymatically cross-linked hyperbranched polyglycerol hydrogels as scaffolds for living cells.
abstract: Although several strategies are now available to enzymatically cross-link linear polymers to hydrogels for biomedical use, little progress has been reported on the use of dendritic polymers for the same purpose. Herein, we demonstrate that horseradish peroxidase (HRP) successfully catalyzes the oxidative cross-linking of a hyperbranched polyglycerol (hPG) functionalized with phenol groups to hydrogels. The tunable cross-linking results in adjustable hydrogel properties. Because the obtained materials are cytocompatible, they have great potential for encapsulating living cells for regenerative therapy. The gel formation can be triggered by glucose and controlled well under various environmental conditions.
authors.affiliation: Institut für Chemie und Biochemie, Freie Universität Berlin , Takustraße 3, 14195 Berlin, Germany.
authors.forename: Changzhu
authors.initials: C
authors.lastname: Wu
authors.forename: Christine
authors.initials: C
authors.lastname: Strehmel
authors.forename: Katharina
authors.initials: K
authors.lastname: Achazi
authors.forename: Leonardo
authors.initials: L
authors.lastname: Chiappisi
authors.forename: Jens
authors.initials: J
authors.lastname: Dernedde
authors.forename: Marga C
authors.initials: MC
authors.lastname: Lensen
authors.forename: Michael
authors.initials: M
authors.lastname: Gradzielski
authors.forename: Marion B
authors.initials: MB
authors.lastname: Ansorge-Schumacher
authors.forename: Rainer
authors.initials: R
authors.lastname: Haag
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D003432:Cross-Linking Reagents / Q000737:chemistry* / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D005990:Glycerol / Q000737:chemistry* / Q000494:pharmacology; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D020100:Hydrogels; D011108:Polymers; C043941:polyglycerol; D005990:Glycerol