title: Effects of simulated weightlessness on cellular morphology and biological characteristics of cell lines SGC-7901 and HFE-145.
abstract: We investigated the effects of simulated weightlessness on cellular morphology, proliferation, cell cycle, and apoptosis of the human gastric carcinoma cell line SGC-7901 and the human gastric normal cell line HFE-145. A rotating clinostat was used to simulate weightlessness. The Image-Pro4.5 image analysis system was used for morphometric analysis. Proliferating cell nuclear antigen expression was examined by immunohistochemical staining. Changes in the cell cycle were examined using a cytometer. Apoptosis was measured using the terminal dUTP nick-end labeling (TUNEL) method. When subjected to simulated weightlessness, the cellular morphology of SGC-7901 cells was changed at 12, 24, 48, and 72 h, cell conversion from the G1 to S phase was blocked, proliferation was inhibited at 48 and 72 h, and the apoptosis index was increased at 72 h. The same changes were observed for HFE-145 cells at 12 h when subjected to simulated weightlessness, but no significant changes were found afterward compared with controls. SGC-7901 cells change their cellular morphology and biological characteristics during clinostat-simulated weightlessness at 72 h, but HFE-145 cells only change at 12 h and adapt to simulated weightlessness after that point.
authors.affiliation: Department of Gastroenterology, Air Force General Hospital of PLA, Beijing, China mingzhucn@126.com.
authors.forename: M
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Gastroenterology, Air Force General Hospital of PLA, Beijing, China.
authors.forename: X W
authors.initials: XW
authors.lastname: Jin
authors.affiliation: Department of Gastroenterology, South Building, Chinese PLA General Hospital, Beijing, China.
authors.forename: B Y
authors.initials: BY
authors.lastname: Wu
authors.affiliation: The 13th Laboratory of Institute of Space Medico-Engineering, Beijing, China.
authors.forename: J L
authors.initials: JL
authors.lastname: Nie
authors.affiliation: The 13th Laboratory of Institute of Space Medico-Engineering, Beijing, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Li
mesh_terms: D000220:Adaptation, Biological; D017209:Apoptosis; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014893:Weightlessness
chemical_list: D018809:Proliferating Cell Nuclear Antigen