title: Identification of a novel HOG1 homologue from an industrial glycerol producer Candida glycerinogenes.
abstract: Candida glycerinogenes, a glycerol production industrial strain with hyperosmo-adaptation can grow well in 15 % (w/v) NaCl or 55 % (w/v) glucose. To understand the osmo-adaptation mechanism in C. glycerinogenes, the mitogen-activated protein kinase HOG1 gene (CgHOG1), which plays an essential role in the yeast hyperosmotic response, was isolated by degenerate PCR and SEFA-Formed Adaptor PCR. The CgHOG1 gene was then transformed in Saccharomyces cerevisiae hog1Δ null mutant, which restored the recombination S. cerevisiae to the wild-type phenotype with osmo-adaptation. To further clarify the function of CgHOG1, the phosphorylation of CgHOG1 and transcription of the glycerol-3-phosphate dehydrogenase gene (GPD1) of the CgHOG1-harbouring S. cerevisiae mutant was detected, and found to be similar to that of wild-type S. cerevisiae. In addition, the recombination S. cerevisiae with CgHOG1 gene significantly accumulated intracellular glycerol when stressed with NaCl.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China, 18352520980@163.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Ji
authors.forename: Xinyao
authors.initials: X
authors.lastname: Lu
authors.forename: Chengyin
authors.initials: C
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Zong
authors.forename: Huiying
authors.initials: H
authors.lastname: Fang
authors.forename: Jin
authors.initials: J
authors.lastname: Sun
authors.forename: Jian
authors.initials: J
authors.lastname: Zhuge
authors.forename: Bin
authors.initials: B
authors.lastname: Zhuge
mesh_terms: D002175:Candida / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D005816:Genetic Complementation Test; D005990:Glycerol / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism*; D009997:Osmotic Pressure; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D012965:Sodium Chloride; D020928:Mitogen-Activated Protein Kinases; D005990:Glycerol