title: Control systems of membrane transport at the interface between the endoplasmic reticulum and the Golgi.
abstract: A fundamental property of cellular processes is to maintain homeostasis despite varying internal and external conditions. Within the membrane transport apparatus, variations in membrane fluxes from the endoplasmic reticulum (ER) to the Golgi complex are balanced by opposite fluxes from the Golgi to the ER to maintain homeostasis between the two organelles. Here we describe a molecular device that balances transport fluxes by integrating transduction cascades with the transport machinery. Specifically, ER-to-Golgi transport activates the KDEL receptor at the Golgi, which triggers a cascade that involves Gs and adenylyl cyclase and phosphodiesterase isoforms and then PKA activation and results in the phosphorylation of transport machinery proteins. This induces retrograde traffic to the ER and balances transport fluxes between the ER and Golgi. Moreover, the KDEL receptor activates CREB1 and other transcription factors that upregulate transport-related genes. Thus, a Golgi-based control system maintains transport homeostasis through both signaling and transcriptional networks.
authors.affiliation: Istituto di Biochimica delle Proteine, Consiglio Nazionale delle Ricerche, Via Pietro Castellino 111, 80131 Napoli, Italy; Telethon Institute of Genetics and Medicine, Via Pietro Castellino 111, 80131 Napoli, Italy; Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Quillota 980, Viña del Mar 2520000, Chile. Electronic address: jorge.cancino@unab.cl.
authors.forename: Jorge
authors.initials: J
authors.lastname: Cancino
authors.affiliation: Istituto di Biochimica delle Proteine, Consiglio Nazionale delle Ricerche, Via Pietro Castellino 111, 80131 Napoli, Italy; Telethon Institute of Genetics and Medicine, Via Pietro Castellino 111, 80131 Napoli, Italy.
authors.forename: Anita
authors.initials: A
authors.lastname: Capalbo
authors.affiliation: Istituto di Biochimica delle Proteine, Consiglio Nazionale delle Ricerche, Via Pietro Castellino 111, 80131 Napoli, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Di Campli
authors.affiliation: Consorzio Mario Negri Sud, Via Nazionale 8/A, 66030 Santa Maria Imbaro (Chieti), Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Giannotta
authors.affiliation: Istituto di Biochimica delle Proteine, Consiglio Nazionale delle Ricerche, Via Pietro Castellino 111, 80131 Napoli, Italy; Telethon Institute of Genetics and Medicine, Via Pietro Castellino 111, 80131 Napoli, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Rizzo
authors.affiliation: Istituto di Biochimica delle Proteine, Consiglio Nazionale delle Ricerche, Via Pietro Castellino 111, 80131 Napoli, Italy; Istituto di Ricovero e Cura a Carattere Scientifico, Istituto di Ricerca Diagnostica e Nucleare (SDN), 80143 Napoli, Italy.
authors.forename: Juan E
authors.initials: JE
authors.lastname: Jung
authors.affiliation: Istituto di Biochimica delle Proteine, Consiglio Nazionale delle Ricerche, Via Pietro Castellino 111, 80131 Napoli, Italy.
authors.forename: Rosaria
authors.initials: R
authors.lastname: Di Martino
authors.affiliation: Istituto di Biochimica delle Proteine, Consiglio Nazionale delle Ricerche, Via Pietro Castellino 111, 80131 Napoli, Italy; Istituto di Ricovero e Cura a Carattere Scientifico, Istituto di Ricerca Diagnostica e Nucleare (SDN), 80143 Napoli, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Persico
authors.affiliation: Institut für Biochemie Charité, Universitätsmedizin Berlin, CrossOver Charitéplatz 1/Sitz, Virchowweg 6, 10117 Berlin, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Heinklein
authors.affiliation: Consorzio Mario Negri Sud, Via Nazionale 8/A, 66030 Santa Maria Imbaro (Chieti), Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Sallese
authors.affiliation: Istituto di Biochimica delle Proteine, Consiglio Nazionale delle Ricerche, Via Pietro Castellino 111, 80131 Napoli, Italy; Telethon Institute of Genetics and Medicine, Via Pietro Castellino 111, 80131 Napoli, Italy. Electronic address: a.luini@ibp.cnr.it.
authors.forename: Alberto
authors.initials: A
authors.lastname: Luini
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D006706:Homeostasis / Q000502:physiology; D006801:Humans; D051379:Mice; D010766:Phosphorylation; D018000:Receptors, Peptide / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology
chemical_list: C064459:KDEL receptor; D018000:Receptors, Peptide