title: Infectious involvement in a tertiary center pediatric uveitis cohort.
abstract: OBJECTIVE
  Studies of uveitis in children have focused primarily on non-infectious causes. To date, no systematic study of infectious uveitis in children has been conducted. We investigate the prevalence of infectious causes of uveitis in children and explore the diagnostic value of analysing aqueous humour.
  
  
  METHODS
  Retrospective cohort study in a tertiary referral centre for paediatric uveitis. Medical records of 345 children with uveitis presenting from 1995 through 2010 were reviewed for infectious causes (by serology and aqueous humour analysis).
  
  
  RESULTS
  A diagnosis of infectious uveitis was established in 60/345 (17%) children. The most prevalent pathogen was Toxoplasma gondii (36/60; 60%), followed by viral infections (18/60; 30%). The most prevalent viral pathogen was varicella-zoster virus (VZV), representing 7/18 (39%) children. Viral causes were less often bilateral than other infectious causes (p=0.04). Specific IgG serum levels determined in 42/60 (70%) patients, were positive in 41/42 (98%). Aqueous humour was analysed for 24/60 (40%) patients and was positive in 18/24 (75%).
  
  
  CONCLUSIONS
  An infectious cause of uveitis was identified in 17% of children with uveitis. T gondii and VZV were the most prevalent pathogens. We recommend analysing the aqueous humour of every child with vision-threatening uveitis of undetermined origin.
authors.affiliation: Department of Ophthalmology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Ymkje Marije
authors.initials: YM
authors.lastname: Hettinga
authors.affiliation: Department of Virology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Jolanda Dorothea Francisca
authors.initials: JD
authors.lastname: de Groot-Mijnes
authors.affiliation: Department of Ophthalmology, Erasmus Medical Center Rotterdam, Rotterdam, The Netherlands.
authors.forename: Aniki
authors.initials: A
authors.lastname: Rothova
authors.affiliation: Department of Ophthalmology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Joke Helena
authors.initials: JH
authors.lastname: de Boer
mesh_terms: D000293:Adolescent; D000818:Animals; D001082:Aqueous Humor / Q000382:microbiology / Q000469:parasitology / Q000821:virology; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D015818:Eye Infections, Bacterial / Q000175:diagnosis* / Q000382:microbiology; D015822:Eye Infections, Parasitic / Q000175:diagnosis* / Q000469:parasitology; D015828:Eye Infections, Viral / Q000175:diagnosis* / Q000821:virology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D010271:Parasites / Q000235:genetics / Q000302:isolation & purification; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D062606:Tertiary Care Centers; D014605:Uveitis / Q000382:microbiology / Q000469:parasitology / Q000821:virology; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification
chemical_list: D007074:Immunoglobulin G
keywords: Anterior chamber; Aqueous humour; Child health (paediatrics); Infection