title: Competitive immunochromatographic assay for the detection of thiodiglycol sulfoxide, a degradation product of sulfur mustard.
abstract: An immunochromatographic assay (ICA) based on the competitive antigen-coated format using colloidal gold as the label was developed for the detection of thiodiglycol sulfoxide (TDGO), an important metabolite and degradation compound of sulphur mustard (SM). The ICA test strip consisted of a membrane with a detection zone, a sample pad and an absorbent pad. The membrane was separately coated with hapten-OVA conjugate (test line) and anti-rabbit mouse IgG (control line). The visual detection limit for TDGO by ICA detection was found to be 10 μg mL(-1). For validation, the ICA results obtained for spiked water samples were in good agreement with those obtained by indirect competitive inhibition enzyme-linked immunosorbent assay (ELISA) for TDGO. The assay time for detection was less than 10 min. The developed ICA has the potential to be a useful on-site screening tool for the retrospective detection of SM in environmental samples.
authors.affiliation: Process Technology Development Division, Discovery Centre, Defense R & D Establishment, Jhansi Road, Gwalior 474002, India. drmanishasathe@drde.drdo.in.
authors.forename: Manisha
authors.initials: M
authors.lastname: Sathe
authors.forename: Shruti
authors.initials: S
authors.lastname: Srivastava
authors.forename: S
authors.initials: S
authors.lastname: Merwyn
authors.forename: G S
authors.initials: GS
authors.lastname: Agarwal
authors.forename: M P
authors.initials: MP
authors.lastname: Kaushik
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002846:Chromatography, Affinity; D006046:Gold / Q000737:chemistry; D006241:Haptens / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D009151:Mustard Gas / Q000737:chemistry / Q000378:metabolism*; D010047:Ovalbumin / Q000737:chemistry; D011817:Rabbits; D013454:Sulfoxides / Q000032:analysis*
chemical_list: D006241:Haptens; D007074:Immunoglobulin G; D013454:Sulfoxides; C073450:2,2'-sulfinylbisethanol; D006046:Gold; D010047:Ovalbumin; D009151:Mustard Gas