title: Tribbles 3 regulates the fibrosis cytokine TGF- β 1 through ERK1/2-MAPK signaling pathway in diabetic nephropathy.
abstract: To reveal the expression and possible role of tribbles homolog 3 (TRB3) in the incidence of type 2 diabetic nephropathy, we used immunohistochemistry, real-time quantitative PCR, western blot analysis, and enzyme-linked immunosorbent assay (ELISA) to study the expression of TRB3, extracellular signal-regulated kinase 1/2 mitogen-activated protein kinase (ERK1/2 MAPK), transforming growth factor β1 (TGF-β1), and collagen type IV in kidneys of db/db diabetic mice and in murine renal mesangial cells stimulated with high glucose. The expression of TRB3, TGF-β1, and collagen type IV was increased in kidneys of db/db diabetic mice. TGF-β1 and collagen type IV regulated by high glucose through ERK1/2 MAPK were downregulated by silencing TRB3 in renal mesangial cells. TRB3 may be involved in diabetic nephropathy by regulating the fibrosis cytokine TGF-β1 and collagen type IV through the ERK1/2 MAPK signaling pathway.
authors.affiliation: Department of Hemodialysis, Qilu Hospital, Shandong University, Jinan 250012, China.
authors.forename: Luwei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Hemodialysis, Qilu Hospital, Shandong University, Jinan 250012, China.
authors.forename: Jinhang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: General Department, Qilu Hospital, Shandong University, Jinan 250012, China.
authors.forename: Xinnong
authors.identifier: 0000-0001-7137-6257
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Hemodialysis, Qilu Hospital, Shandong University, Jinan 250012, China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Hemodialysis, Qilu Hospital, Shandong University, Jinan 250012, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tian
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D024141:Collagen Type IV / Q000235:genetics / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications; D003928:Diabetic Nephropathies / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D004195:Disease Models, Animal; D005355:Fibrosis; D015870:Gene Expression; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D007678:Kidney Glomerulus / Q000378:metabolism / Q000473:pathology; D050527:Mesangial Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D015398:Signal Transduction / Q000187:drug effects; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D024141:Collagen Type IV; C474830:TRB3 protein, mouse; D053773:Transforming Growth Factor beta1; D005947:Glucose