title: Prussian blue-Au nanocomposites actuated hemin/G-quadruplexes catalysis for amplified detection of DNA, Hg2+ and adenosine triphosphate.
abstract: In this paper, horseradish peroxidase-mimicking DNAzyme (HRP-DNAzyme) and Prussian blue (PB)-gold (Au) nanocomposites were designed as versatile electrochemical sensing platforms for the amplified detection of DNA, Hg(2+) and adenosine triphosphate (ATP). By the conjugation of the target probe with the capture probe, a conformational change resulted in the formation of HRP-DNAzyme on the PB-Au modified electrode. The redox of HRP-DNAzyme (red) was efficiently carried out in the presence of H2O2, in which PB acted as a mediator stimulating the biocatalytic functions of HRP-DNAzyme and actuated a catalytic cycle bringing an amplified signal. Specific recognition of the target DNA, Hg(2+) and ATP allowed selective amperometric detection of the target molecule. The detection limits of DNA, Hg(2+) and ATP were 50 nM, 30 pM and 3 nM, respectively. The highlight of this work is that the catalytic cycle between PB-Au nanocomposites and HRP-DNAzyme was adequately utilized in the amplification platform for versatile sensing. The novel electrocatalytic biosensor involving only one-step incubation exhibited a wide linear range, low detection limit, and satisfactory selectivity and operational stability. The proposed approach provided an ease-of-use and universal reporting system with a simple design and easy operations.
authors.affiliation: Key Laboratory of Chem-Biosensing, Key Laboratory of Functional Molecular Solids, College of Chemistry and Materials Science, Center for Nano Science and Technology, Anhui Normal University, Wuhu 241000, Anhui, PR China.
authors.forename: Guangfeng
authors.initials: G
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xiuping
authors.initials: X
authors.lastname: He
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D002384:Catalysis; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004247:DNA / Q000032:analysis*; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism; D055664:Electrochemical Techniques; D004566:Electrodes; D005295:Ferrocyanides / Q000737:chemistry; D054856:G-Quadruplexes; D006046:Gold / Q000737:chemistry; D006427:Hemin / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D008628:Mercury / Q000032:analysis*; D053761:Nanocomposites / Q000737:chemistry*
chemical_list: D021881:DNA, Catalytic; D005295:Ferrocyanides; D007477:Ions; D006427:Hemin; D006046:Gold; D000255:Adenosine Triphosphate; D004247:DNA; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D008628:Mercury; C000170:ferric ferrocyanide