title: Possible role of fat tissue in the pharmacokinetics of Dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamides after oral administration of Echinacea angustifolia extract in rats.
abstract: Alkamides are one of the most important constituents of lipophilic extracts of Echinacea angustifolia roots. These compounds play an important role in the versatile pharmacological actions of this plant. The present study aimed to compare the concentrations of isomeric dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamides (DTAI) in brain and periepididymal fat tissues and blood plasma of rats. Thirty, 60, 240 and 720 min after the oral administration of E. angustifolia root extract, tissue and plasma concentrations were determined by reversed-phase HPLC with ESI-MS/MS detection. The calculated terminal t1/2 of the mixture of DTAI was 8.28 h, which indicates a relatively slow elimination. In the 0.5-4 h period the brain/plasma and fat/plasma concentration ratios were continuously above 3 and 18, respectively, followed by equilibrium at 12 h. Our results indicate substantial accumulation of alkamides in lipid-rich tissues, which presumably contributes to a maintained pharmacological action.
authors.forename: Nikoletta
authors.initials: N
authors.lastname: Jedlinszki
authors.forename: Dóra
authors.initials: D
authors.lastname: Rédei
authors.forename: József
authors.initials: J
authors.lastname: Haller
authors.forename: Tamás F
authors.initials: TF
authors.lastname: Freund
authors.forename: Judit
authors.initials: J
authors.lastname: Hohmann
authors.forename: István
authors.initials: I
authors.lastname: Zupkó
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D020900:Echinacea / Q000737:chemistry*; D008297:Male; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D053284:Polyunsaturated Alkamides / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010936:Plant Extracts; D053284:Polyunsaturated Alkamides