title: PIXSIC, a pixelated β⁺-sensitive probe for radiopharmacological investigations in rat brain: binding studies with [¹⁸F]MPPF.
abstract: OBJECTIVE
  The aim of this work was to demonstrate the pharmacokinetic potential of a wireless pixelated β(+)-sensitive probe (PIXSIC).
  
  
  METHODS
  The binding of 2'-methoxyphenyl-(N-2'-pyridinyl)-p-[(18)F]fluoro-benzamidoethylpiperazine ([(18)F]MPPF), a 5-HT1A serotonin receptor radiopharmaceutical, was measured in anesthetized rats and compared to microPET data. The effects of a 5-HT1A antagonist injection on in vivo [(18)F]MPPF binding were monitored by PIXSIC.
  
  
  RESULTS
  PIXSIC allowed differentiating the radioactive kinetics according to the location of its pixels in the hippocampus, cortex, corpus callosum, and cerebellum. The device accurately detected the changes in [(18)F]MPPF binding, after 5-HT1A antagonist blockade. The time-activity curves were reproducible and consistent with kinetics obtained simultaneously with a microPET camera.
  
  
  CONCLUSIONS
  These results demonstrate the ability of the PIXSIC device to record reliably the binding of PET ligands, with a high spatiotemporal resolution in anesthetized rodents. These first in vivo results are a key stage on the path to its implementation in awake freely moving animals.
authors.affiliation: IMNC Laboratory, Université Paris Sud, Orsay, France.
authors.forename: L
authors.initials: L
authors.lastname: Balasse
authors.forename: J
authors.initials: J
authors.lastname: Maerk
authors.forename: F
authors.initials: F
authors.lastname: Pain
authors.forename: A
authors.initials: A
authors.lastname: Genoux
authors.forename: S
authors.initials: S
authors.lastname: Fieux
authors.forename: C
authors.initials: C
authors.lastname: Morel
authors.forename: P
authors.initials: P
authors.lastname: Gisquet-Verrier
authors.forename: L
authors.initials: L
authors.lastname: Zimmer
authors.forename: P
authors.initials: P
authors.lastname: Lanièce
mesh_terms: D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000000981:diagnostic imaging*; D002525:Cerebellar Cortex / Q000000981:diagnostic imaging; D002531:Cerebellum / Q000000981:diagnostic imaging; D006624:Hippocampus / Q000000981:diagnostic imaging; D007275:Injections, Intravenous; D007700:Kinetics; D010879:Piperazines; D049268:Positron-Emission Tomography; D011725:Pyridines; D051381:Rats; D059015:Wireless Technology
chemical_list: D010879:Piperazines; D011725:Pyridines; C416325:4-(2'-methoxyphenyl)-1-(2'-(N-2'-pyridinyl)-p-(18F)fluorobenzamido )ethylpiperazine