title: Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies.
abstract: BACKGROUND
  With the accelerating development of bioscience, the problem of research cost has become important. We previously devised and developed a novel concept microarray with manageable volumes (MMV) using a soft gel. It demonstrated the great potential of the MMV technology with the examples of 1024-parallel-cell culture and PCR experiments. However, its full potential failed to be expressed, owing to the nature of the material used for the MMV chip.
  
  
  RESULTS
  In the present study, by developing plastic-based MMVs and associated technologies, we introduced novel technologies such as C2D2P (in which the cells in each well are converted from DNA to protein in 1024-parallel), NGS-non-dependent microbiome analysis, and other powerful applications.
  
  
  CONCLUSIONS
  The reborn MMV-microarray technology has proven to be highly efficient and cost-effective (with approximately 100-fold cost reduction) and enables us to realize hitherto unattainable technologies.
authors.forename: Harshita
authors.initials: H
authors.lastname: Sharma
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Kinoshita
authors.forename: Seiichi
authors.initials: S
authors.lastname: Fujiu
authors.forename: Shota
authors.initials: S
authors.lastname: Nomura
authors.forename: Mizuho
authors.initials: M
authors.lastname: Sawada
authors.forename: Shamim
authors.initials: S
authors.lastname: Ahmed
authors.forename: Masaki
authors.initials: M
authors.lastname: Shibuya
authors.forename: Kosaku
authors.initials: K
authors.lastname: Shirai
authors.forename: Syota
authors.initials: S
authors.lastname: Takamatsu
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Yamazaki
authors.forename: Ryohei
authors.initials: R
authors.lastname: Kamiyama
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Hidenao
authors.initials: H
authors.lastname: Arai
authors.forename: Miho
authors.initials: M
authors.lastname: Suzuki
authors.forename: Naoto
authors.initials: N
authors.lastname: Nemoto
authors.forename: Ki
authors.initials: K
authors.lastname: Ando
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Uchida
authors.forename: Koichiro
authors.initials: K
authors.lastname: Kitamura
authors.forename: Osamu
authors.initials: O
authors.lastname: Takei
authors.affiliation: Department of Functional Materials Science, Graduate School of Science and Engineering, Saitama University, 255 Shimo-okubo, Saitama 338-8570, Japan. koichi@fms.saitama-u.ac.jp.
authors.forename: Koichi
authors.initials: K
authors.lastname: Nishigaki
mesh_terms: D046228:Microarray Analysis / Q000191:economics / Q000295:instrumentation* / Q000379:methods*; D064307:Microbiota; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results