title: Placental lipases in pregnancies complicated by gestational diabetes mellitus (GDM).
abstract: Infants of women with gestational diabetes mellitus (GDM) are more likely to be born large for gestational age with a higher percentage body fat. Elevated maternal lipids may contribute to this. Placental lipases such as lipoprotein lipase (LPL), endothelial lipase (EL) and hormone sensitive lipase (HSL) are involved in transferring lipids from mother to fetus. Previous studies of expression of these lipases in placentae in women with diabetes in pregnancy have reported divergent results. Intracellular lipases such as adipose triglyceride lipase (ATGL), and HSL are central to lipid droplet metabolism. The activities of these lipases are both influenced by Perilipin 1, and ATGL is also activated by a co-factor comparative gene identification-58 (CGI-58) and inhibited by G0/G1 switch gene 2 (GS02). None of these modifying factors or ATGL have been examined previously in placenta. The purpose of this study was therefore to examine the expression of ATGL, HSL, LPL, EL, as well as Perilipin 1, GS02 and CGI-58 in term pregnancies complicated by GDM. mRNA and protein expression of the lipases were measured in placentae from 17 women with GDM and 17 normoglycaemic pregnancies, matched for maternal BMI and gestational age of delivery. ATGL mRNA expression was increased and HSL mRNA expression reduced in placentae from GDM although there was no differences in protein expression of any of the lipases. All lipases were localised to trophoblasts and endothelial cells. The expression of Perilipin 1 and CGI-58 mRNA was increased and GS02 not altered in GDM. These results suggest that there is no difference in expression in these four lipases between GDM and normoglycaemic placentae, and therefore altered lipid transfer via these lipases does not contribute to large for gestational age in infants of women with GDM.
authors.affiliation: UQ Centre for Clinical Research, University of Queensland, Herston, QLD, Australia; Royal Brisbane and Women's Hospital, Herston, QLD, Australia; School of Medicine, University of Queensland, Herston, QLD, Australia.
authors.forename: Helen L
authors.initials: HL
authors.lastname: Barrett
authors.affiliation: UQ Centre for Clinical Research, University of Queensland, Herston, QLD, Australia.
authors.forename: Marta H
authors.initials: MH
authors.lastname: Kubala
authors.affiliation: UQ Centre for Clinical Research, University of Queensland, Herston, QLD, Australia.
authors.forename: Katherin
authors.initials: K
authors.lastname: Scholz Romero
authors.affiliation: School of Biomedical Sciences, University of Queensland, St Lucia, QLD, Australia.
authors.forename: Kerina J
authors.initials: KJ
authors.lastname: Denny
authors.affiliation: School of Biomedical Sciences, University of Queensland, St Lucia, QLD, Australia.
authors.forename: Trent M
authors.initials: TM
authors.lastname: Woodruff
authors.affiliation: School of Medicine, University of Queensland, Herston, QLD, Australia; Mater Research Institute, University of Queensland, Brisbane, QLD, Australia.
authors.forename: H David
authors.initials: HD
authors.lastname: McIntyre
authors.affiliation: Royal Brisbane and Women's Hospital, Herston, QLD, Australia; School of Medicine, University of Queensland, Herston, QLD, Australia.
authors.forename: Leonie K
authors.initials: LK
authors.lastname: Callaway
authors.affiliation: UQ Centre for Clinical Research, University of Queensland, Herston, QLD, Australia; School of Medicine, University of Queensland, Herston, QLD, Australia.
authors.forename: Marloes Dekker
authors.initials: MD
authors.lastname: Nitert
mesh_terms: D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase / Q000235:genetics / Q000378:metabolism; D000328:Adult; D001724:Birth Weight; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D016640:Diabetes, Gestational / Q000201:enzymology* / Q000235:genetics; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007231:Infant, Newborn; D008049:Lipase / Q000235:genetics / Q000378:metabolism*; D050356:Lipid Metabolism; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism; D008297:Male; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D002787:Sterol Esterase / Q000235:genetics / Q000378:metabolism; D000217:Acyltransferases
chemical_list: D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D008049:Lipase; D008071:Lipoprotein Lipase; D000070778:Perilipin-1; D010750:Phosphoproteins; D012333:RNA, Messenger; D002787:Sterol Esterase; C508073:G0S2 protein, human; C000602978:PLIN1 protein, human; C439972:ABHD5 protein, human; C503451:LIPA protein, human; C410382:LIPG protein, human; C494794:PNPLA2 protein, human; D000217:Acyltransferases; C548261:LPL protein, human