title: A novel electrochemiluminescence tetracyclines sensor based on a Ru(bpy)₃²⁺-doped silica nanoparticles/Nafion film modified electrode.
abstract: A novel method for the determination of tetracyclines (TCs) using electrochemiluminescence (ECL) based on a Ru(bpy)3(2+)-doped silica nanoparticles (RuSiNPs)/Nafion film modified electrode is presented in this paper. The RuSiNPs were prepared by a water-in-oil microemulsion method. The characterization results indicated that the thus-prepared RuSiNPs presented a uniform size of 45 nm and retained the original electrochemical properties of Ru(bpy)3(2+). Importantly, the ECL response on RuSiNPs/Nafion film modified electrode was greatly enhanced by TCs. Under the optimum conditions, the ECL intensity versus the concentration of TCs was found to be linear over the range of 1-100 μmol L(-1) for tetracycline (TC), 0.1-100 μmol L(-1) for oxytetracycline (OTC) and 1-100 μmol L(-1) for chlortetracycline (CTC). The detection limits (S/N=3) were 0.23 μmol L(-1) for TC, 0.10 μmol L(-1) for OTC and 0.16 μmol L(-1) for CTC. Moreover, due to the electrostatic interaction between Ru(bpy)3(2+) and silica matrix, the leaching of Ru(bpy)3(2+) was greatly reduced, therefore, the ECL sensor exhibited excellent repeatability and stability in the detection of TCs. Based on these investigations, the proposed ECL approach was successfully used to analyze the TCs content in drugs.
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Zhao
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Tian
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
authors.forename: Sai
authors.initials: S
authors.lastname: Lian
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, China. Electronic address: zhyhuang@jmu.edu.cn.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
mesh_terms: D002751:Chlortetracycline / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D055664:Electrochemical Techniques; D004566:Electrodes; D005465:Fluorocarbon Polymers / Q000737:chemistry*; D016014:Linear Models; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods; D008670:Metals / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods; D009821:Oils / Q000737:chemistry; D010118:Oxytetracycline / Q000737:chemistry; D015203:Reproducibility of Results; D012413:Rubidium / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D013754:Tetracyclines / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D005465:Fluorocarbon Polymers; D008670:Metals; D009821:Oils; D013754:Tetracyclines; D014867:Water; C040402:perfluorosulfonic acid; D012822:Silicon Dioxide; D012413:Rubidium; D002751:Chlortetracycline; D010118:Oxytetracycline; D012825:Silicon
keywords: Electrochemiluminescence; Ru(bpy)(3)(2+)-doped silica; Sensor; Tetracyclines