title: Spectroscopic analyses and studies on respective interaction of cyanuric acid and uric acid with bovine serum albumin and melamine.
abstract: In this work, the fluorescence quenching was used to study the interaction of cyanuric acid (CYA) and uric acid (UA) with bovine serum albumin (BSA) at two different temperatures (283 K and 310 K). The bimolecular quenching constant (Kq), apparent quenching constant (Ksv), effective binding constant (KA) and corresponding dissociation constant (KD), binding site number (n) and binding distance (r) were calculated by adopting Stern-Volmer, Lineweaver-Burk, Double logarithm and overlap integral equations. The results show that CYA and UA are both able to obviously bind to BSA, but the binding strength order is BSA+CYA<BSA+UA. And then, the interactions of CYA and UA with melamine (MEL) under the same conditions were also studied by using similar methods. The results indicates that both CYA and UA can bind together closely with melamine (MEL). It is wished that these research results would facilitate the understanding the formation of kidney stones and gout in the body after ingesting excess MEL.
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, PR China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Chen
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, PR China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, PR China. Electronic address: wangjun888tg@126.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Environment, Liaoning University, Shenyang 110036, PR China.
authors.forename: Heng
authors.initials: H
authors.lastname: Qiao
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004735:Energy Transfer; D007700:Kinetics; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014227:Triazines / Q000737:chemistry / Q000378:metabolism*; D014527:Uric Acid / Q000737:chemistry / Q000378:metabolism*
chemical_list: D012996:Solutions; D014227:Triazines; D014527:Uric Acid; D012710:Serum Albumin, Bovine; C004632:cyanuric acid; C011907:melamine
keywords: Bovine serum albumin (BSA); Fluorescence spectroscopy; Interaction; Melamine, cyanuric acid and uric acid