title: Innate-like and conventional T cell populations from hemodialyzed and kidney transplanted patients are equally compromised.
abstract: Clinicians are well aware of existing pharmacologically-induced immune deficient status in kidney-transplanted patients that will favor their susceptibility to bacterial or viral infections. Previous studies indicated that advanced Stage 4-5 Chronic Kidney Disease might also be regarded as an immune deficiency-like status as well, even though the mechanisms are not fully understood. Here, we analyzed the ex vivo frequency and the functional properties of both conventional and innate-like T (ILT) lymphocyte subsets in the peripheral blood of 35 patients on hemodialysis, 29 kidney transplanted patients and 38 healthy donors. We found that peripheral blood cell count of ILT cells, as iNKT (invariant Natural Killer T) and MAIT (mucosal-associated invariant T), were significantly decreased in hemodialyzed patients compared to healthy controls. This deficiency was also observed regarding conventional T cells, including the IL-17-producing CD4(+) Th17 cells. Pertaining to regulatory T cells, we also noticed major modifications in the global frequency of CD4(+)CD25(+)Foxp3(+) T lymphocytes, including the resting suppressive CD45RA(+)Foxp3lo and activated suppressive CD45RA-Foxp3hi T cell subpopulations. We found no significant differences between the immune status of hemodialyzed and kidney-transplanted subjects. In conclusion, we demonstrated that both ILT and conventional T cell numbers are equally impaired in hemodialyzed and kidney-transplanted patients.
authors.affiliation: INEM (Institut Necker Enfants Malades), CNRS UMR 8253, INSERM UMR 1151, Université Paris Descartes, Sorbonne Paris Cité, France, Hôpital Necker Enfants Malades, Paris, France.
authors.forename: Marine
authors.initials: M
authors.lastname: Baron
authors.affiliation: INEM (Institut Necker Enfants Malades), CNRS UMR 8253, INSERM UMR 1151, Université Paris Descartes, Sorbonne Paris Cité, France, Hôpital Necker Enfants Malades, Paris, France.
authors.forename: Renata
authors.initials: R
authors.lastname: Belo
authors.affiliation: INSERM UMR S 702, Université Pierre et Marie Curie, Assistance Publique-Hôpitaux de Paris, Hôpital Tenon, Paris, France; AP-HP, Hôpital Tenon, Urgences Néphrologiques & Transplantation Rénale, Universite Pierre et Marie Curie, Paris, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Cathelin
authors.affiliation: INEM (Institut Necker Enfants Malades), CNRS UMR 8253, INSERM UMR 1151, Université Paris Descartes, Sorbonne Paris Cité, France, Hôpital Necker Enfants Malades, Paris, France.
authors.forename: Lucia
authors.initials: L
authors.lastname: Moreira-Teixeira
authors.affiliation: Nephrology and Dialysis, Tenon Hospital, Assistance Publique-Hôpitaux de Paris, Hôpital Tenon, Paris, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Cartery
authors.affiliation: INSERM UMR S 702, Université Pierre et Marie Curie, Assistance Publique-Hôpitaux de Paris, Hôpital Tenon, Paris, France; AP-HP, Hôpital Tenon, Urgences Néphrologiques & Transplantation Rénale, Universite Pierre et Marie Curie, Paris, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Rondeau
authors.affiliation: INSERM UMR S 702, Université Pierre et Marie Curie, Assistance Publique-Hôpitaux de Paris, Hôpital Tenon, Paris, France; AP-HP, Hôpital Tenon, Urgences Néphrologiques & Transplantation Rénale, Universite Pierre et Marie Curie, Paris, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Mesnard
authors.affiliation: INEM (Institut Necker Enfants Malades), CNRS UMR 8253, INSERM UMR 1151, Université Paris Descartes, Sorbonne Paris Cité, France, Hôpital Necker Enfants Malades, Paris, France.
authors.forename: Maria
authors.initials: M
authors.lastname: Leite-de-Moraes
mesh_terms: D000328:Adult; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006801:Humans; D007113:Immunity, Innate; D016867:Immunocompromised Host; D016130:Immunophenotyping; D007676:Kidney Failure, Chronic / Q000209:etiology / Q000276:immunology / Q000628:therapy; D016030:Kidney Transplantation; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D006435:Renal Dialysis; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D055815:Young Adult
chemical_list: D016207:Cytokines