title: Molecular self assembly of mixed comb-like dextran surfactant polymers for SPR virus detection.
abstract: The synthesis of two comb-like dextran surfactant polymers, that are different in their dextran molecular weight (MW) distribution and the presence of carboxylic groups, and their characterization are reported. A bimodal carboxylic dextran surfactant polymer consists of poly(vinyl amine) (PVAm) backbone with carboxyl higher MW dextran, non-functionalized lower MW dextran and hydrophobic hexyl branches; while a monomodal dextran surfactant polymer is PVAm grafted with non-functionalized lower MW dextran and hexyl branches. Layer formation of non-covalently attached dextran chains with bimodal MW distributions on a surface plasmon resonance (SPR) chip was investigated from the perspective of mixed physisorption of the bimodal and monomodal surfactant polymers. Separation distances between the carboxylic longer dextran side chains within the bimodal surfactant polymer and between the whole bimodal surfactant molecules on the chip surface could be well-controlled. SPR analysis of shrimp yellow head virus using our mixed surfactant chips showed dependence on synergetic adjustment of these separation distances.
authors.affiliation: National Metal and Materials Technology Center, 114 Thailand Science Park, Paholyothin Rd., Klong 1, Klong Luang, Pathumthani 12120, Thailand. Electronic address: Katanchalee.Mai-ngam@nstda.or.th.
authors.forename: Katanchalee
authors.initials: K
authors.lastname: Mai-Ngam
authors.affiliation: CENTEX Shrimp, Faculty of Science, Mahidol University, Rama 6 Rd., Bangkok 10400, Thailand; National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Paholyothin Rd., Klong 1, Klong Luang, Pathumthani 12120, Thailand. Electronic address: wansika@biotec.or.th.
authors.forename: Wansika
authors.initials: W
authors.lastname: Kiatpathomchai
authors.affiliation: CENTEX Shrimp, Faculty of Science, Mahidol University, Rama 6 Rd., Bangkok 10400, Thailand; National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Paholyothin Rd., Klong 1, Klong Luang, Pathumthani 12120, Thailand. Electronic address: narong.aru@biotec.or.th.
authors.forename: Narong
authors.initials: N
authors.lastname: Arunrut
authors.affiliation: National Metal and Materials Technology Center, 114 Thailand Science Park, Paholyothin Rd., Klong 1, Klong Luang, Pathumthani 12120, Thailand. Electronic address: jitladas@mtec.or.th.
authors.forename: Jitlada
authors.initials: J
authors.lastname: Sansatsadeekul
mesh_terms: D000818:Animals; D003911:Dextrans / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D018625:Microscopy, Atomic Force; D008970:Molecular Weight; D033561:Penaeidae / Q000821:virology; D046828:Roniviridae / Q000302:isolation & purification*; D020349:Surface Plasmon Resonance / Q000379:methods*; D013501:Surface-Active Agents / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D003911:Dextrans; D013501:Surface-Active Agents
keywords: Bimodal molecular weight distribution; Dextran surfactant polymer; Surface plasmon resonance; Virus