title: Expression of vesicular glutamate transporter 3 mRNA in the brain and retina of the pigeon.
abstract: Vesicular glutamate transporters (vGluTs), which accumulate glutamate into synaptic vesicles, are classified into three subtypes in mammalian brains: vGluT1, vGluT2, and vGluT3. VGluT3 is localized in non-glutamatergic neurons of the brain and retinal amacrine cells. In birds, the vGluT3 genome is found, but its distribution in the brain or retina is unknown. The present study was conducted to analyze vGluT3 cDNA sequence and elucidate its distribution in the pigeon brain and retina. The vGluT3 cDNA comprises 1761bp and showed 95% and 88% identity to the chicken and zebra finch vGluT3 cDNAs, respectively, and 74% identity to human vGluT3 cDNA. In situ hybridization revealed that the vGluT3 mRNA was expressed in neurons of the caudal linear nucleus (LC) of the brain and in amacrine cells of the inner nuclear layer of the retina. A combination of in situ hybridization and serotonin immunohistochemistry revealed three types of stained cells in LC and retina: vGluT3(+)/serotonin(+), vGluT3(+)/serotonin(-), and vGluT3(-)/serotonin(+). The vGluT3(+)/serotonin(+) cells were approximately 22% in LC and 16% in the retina. The present results suggest that the pigeon vGluT3 mRNA is comparable with the mammalian type.
authors.affiliation: Laboratory of Veterinary Anatomy, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan. Electronic address: atozi@gifu-u.ac.jp.
authors.forename: Yasuro
authors.initials: Y
authors.lastname: Atoji
authors.affiliation: Laboratory of Veterinary Anatomy, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan; Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
authors.forename: Mohammad Rabiul
authors.initials: MR
authors.lastname: Karim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism*; D010856:Columbidae; D005260:Female; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D012160:Retina / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050496:Vesicular Glutamate Transport Proteins / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D012333:RNA, Messenger; D050496:Vesicular Glutamate Transport Proteins
keywords: Amacrine cell; Bird; Caudal linear nucleus; Cloning; Serotonin