title: Genetic diversity and population structure in Harpadon nehereus based on sequence-related amplified polymorphism markers.
abstract: In this study, the genetic diversity among ten populations of the Bombay duck was studied on the basis of sequence-related amplified polymorphism (SRAP). The ten populations were collected from the East China Sea and South China Sea areas. A total of 98 loci were obtained from 292 individuals using eight SRAP primers. The average proportion of polymorphic loci, genetic diversity (H), and Shannon's information index were 75.20%, 0.2478, and 0.3735, respectively. Nei's genetic distance and Shannon's information index between the ten populations ranged from 0.0410 to 0.3841 and from 0.2396 to 0.4506, and the averages Nei's gene diversity index (H = 0.2478) and Shannon's information index (I = 0.3735) at the population level were high. AMOVA showed that most of the variation was within populations (71.74%), and only 28.26% of the variation was between populations. The neighbor-joining tree based on genetic distance revealed that significant genealogical structure existed throughout the examined range of the Bombay duck. The results demonstrated that SRAP marker was an effective tool for the assessment of genetic diversity in the Bombay duck. The results could be used for further protection of the germplasm resource of the Bombay duck.
authors.affiliation: Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Zhu
authors.affiliation: Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Li
authors.affiliation: Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, China wangrixin1123@126.com.
authors.forename: R X
authors.initials: RX
authors.lastname: Wang
mesh_terms: D000818:Animals; D002681:China; D019143:Evolution, Molecular; D005399:Fishes / Q000235:genetics*; D056426:Genetic Loci; D005819:Genetic Markers; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D021141:Nucleic Acid Amplification Techniques; D010802:Phylogeny; D058974:Phylogeography
chemical_list: D005819:Genetic Markers