title: [Anaplastic lymphoma kinase fusion gene expression, clinical pathological characteristics and prognosis in 95 Chinese patients with non-small cell lung cancer].
abstract: OBJECTIVE
  To examine the prevalence of anaplastic lymphoma kinase (ALK) fusion gene in Chinese patients with non-small cell lung cancer (NSCLC).
  
  
  METHODS
  In this study, 95 patients with NSCLC and corresponding clinical information and formalin-fixed paraffin-embedded (FFPE) tissue blocks were included. Hematoxylin & eosin (HE) staining, conventional ALK immunochemistry (IHC) staining and intercalated antibody-enhanced polymer (iAEP) IHC staining, and dual-color split fluorescence in situ hybridization (FISH) for ALK fusion gene were performed.
  
  
  RESULTS
  Eight ALK-positive cases were detected using anti-ALK immunohistochemistry with the iAEP method, and FISH analyses revealed 4 patients of them who harbored the ALK fusion gene (4.2%, 4/95), including 2 cases of female patients with solid signet-ring cell adenocarcinoma and 2 cases of male patients with adenosquamous carcinoma. The positive cases were all non-smokers without EGFR/KRAS mutations. Furthermore, the positive cases all survived, and the overall postsurgery survival time of 2 cases was more than 5 years.
  
  
  CONCLUSIONS
  ALK IHC with the iAEP method is better than conventional ALK IHC, and the percentage of the positive cells is more important than that of the intensity. ALK translocations were infrequent in the entire NSCLC patient population (<5%) with better prognosis.
authors.affiliation: Department of Pathology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
authors.forename: Meng-xue
authors.initials: MX
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Pei
authors.affiliation: Department of Radiology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Tian-li
authors.initials: TL
authors.lastname: Wang
authors.affiliation: Department of Respiratory Medicine, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Pathology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
authors.forename: Jiang-feng
authors.initials: JF
authors.lastname: You
authors.affiliation: Department of Pathology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
authors.forename: Peng-cheng
authors.initials: PC
authors.lastname: Zou
authors.affiliation: Department of Pathology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
authors.forename: Yue-qi
authors.initials: YQ
authors.lastname: Wang
authors.affiliation: Department of Pathology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
authors.forename: Xu-wen
authors.initials: XW
authors.lastname: Li
authors.affiliation: Department of Pathology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Pathology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
authors.forename: Hao-hao
authors.initials: HH
authors.lastname: Zhong
authors.affiliation: Department of Pathology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pathology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
authors.forename: Yu-xiang
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Department of Pathology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Pathology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000077548:Anaplastic Lymphoma Kinase; D044466:Asian People; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D009154:Mutation; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D015995:Prevalence; D011379:Prognosis; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D014178:Translocation, Genetic
chemical_list: D015514:Oncogene Proteins, Fusion; C000626173:ALK protein, human; D000077548:Anaplastic Lymphoma Kinase; D020794:Receptor Protein-Tyrosine Kinases