title: The "Pavia model" of experimental small bowel transplantation in pigs: technical variations for ischemia reperfusion injury studies.
abstract: Ischemia reperfusion injury (IRI) is a major field of study in small bowel transplantation because of its implications regarding intestinal immunity. In this study, we have introduced some variations to the described models of IRI in pigs to make possible a complete isolation of the small bowel for IRI studies. In swine, two anatomical barriers make impossible a complete isolation of the small bowel at the origin of superior mesenteric artery (SMA) and vein (SMV): the main colic vessels, which originate distally to form SMA and SMV, and the blood supply of the distal portion of the duodenum and the cephalic part of the pancreas. In a group of Large White pigs (n = 5), we have performed a complete isolation of the small bowel, including sub-total colectomy and pancreaticoduodenectomy. Both SMA and SMV were isolated at the origin from the aorta and at the junction of the splenic vein, respectively. Intestinal continuity was restored with duodenojejunal anastomosis and with ileotransverse colon anastomosis. One pig died on postoperative day 5 from intestinal occlusion due to adhesions. The remaining four pigs were killed on postoperative day 7 after an uneventful postoperative course. No complications were found at autopsy. In swine, resection of part of the pancreas and duodenum and removal of the large bowel does not affect short-term survival, allowing a full isolation of the entire small bowel mimicking the transplantation procedure. Thus, this model appears to be attractive for IRI studies in the field of intestinal transplantation.
authors.affiliation: Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy. Electronic address: m.alessiani@unipv.it.
authors.forename: M
authors.initials: M
authors.lastname: Alessiani
authors.affiliation: Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy; Service of General Surgery, Pavia, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Cobianchi
authors.affiliation: Service of General Surgery, Pavia, Italy.
authors.forename: J
authors.initials: J
authors.lastname: Viganò
authors.affiliation: Service of General Surgery, Pavia, Italy.
authors.forename: T
authors.initials: T
authors.lastname: Dominioni
authors.affiliation: Service of Anesthesiology, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Bottazzi
authors.affiliation: Service of General Surgery, Pavia, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Zonta
authors.affiliation: Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy; Service of General Surgery, Pavia, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Dionigi
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007421:Intestine, Small / Q000098:blood supply / Q000637:transplantation*; D017538:Mesenteric Artery, Superior / Q000601:surgery*; D015427:Reperfusion Injury / Q000517:prevention & control*; D034421:Sus scrofa; D013552:Swine; D014182:Transplantation, Autologous