title: Glucose biosensor based on multisegment nanowires exhibiting reversible magnetic control.
abstract: We describe the amperometric detection of glucose using oriented nanowires with magnetic switching of the bioelectrochemical process. The fabrication process of the nanowires was prepared through controlled nucleation and growth during a stepwise electrochemical deposition, and it was characterized using scanning electron microscopy. Cyclic voltammetry and amperometry were used to study the magnetoswitchable property; this control was accomplished by changing the surface orientation of nanowires. Under the optimal condition, the amperometric response was also linear up to a glucose concentration of 0.1-16.0 mmol L(-1) with a sensitivity of 81 μA mM(-1). The detection limit was estimated for 4.8×10(-8) mol L(-1), defined from a signal/noise ratio of 3. It also exhibits good reproducibility and high selectivity with insignificant interference from ascorbic acid, acetoaminophen, and uric acid. The resulting biosensor was applied to detect the blood sugar in human serum samples without any pretreatment, and the results were comparatively in agreement with the clinical assay.
authors.affiliation: Institute of Bioscience, Department of Chemistry and Biochemistry, UNESP, Botucatu-SP, Brazil.
authors.forename: Gislaine P
authors.initials: GP
authors.lastname: Gerola
authors.affiliation: Institute of Bioscience, Department of Chemistry and Biochemistry, UNESP, Botucatu-SP, Brazil.
authors.forename: Giovanna S
authors.initials: GS
authors.lastname: Takahashi
authors.affiliation: Institute of Bioscience, Department of Chemistry and Biochemistry, UNESP, Botucatu-SP, Brazil.
authors.forename: Geraldo G
authors.initials: GG
authors.lastname: Perez
authors.affiliation: Institute of Bioscience, Department of Chemistry and Biochemistry, UNESP, Botucatu-SP, Brazil.
authors.forename: Lucas C
authors.initials: LC
authors.lastname: Recco
authors.affiliation: Institute of Bioscience, Department of Chemistry and Biochemistry, UNESP, Botucatu-SP, Brazil. Electronic address: vpedrosa@ibb.unesp.br.
authors.forename: Valber A
authors.initials: VA
authors.lastname: Pedrosa
mesh_terms: D000082:Acetaminophen / Q000737:chemistry; D001205:Ascorbic Acid / Q000737:chemistry; D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D002384:Catalysis; D055664:Electrochemical Techniques; D004566:Electrodes; D005949:Glucose Oxidase / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008280:Magnetics; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology / Q000379:methods*; D053770:Nanowires / Q000737:chemistry*; D015203:Reproducibility of Results; D014527:Uric Acid / Q000737:chemistry
chemical_list: D001786:Blood Glucose; D014527:Uric Acid; D000082:Acetaminophen; D005949:Glucose Oxidase; D001205:Ascorbic Acid
keywords: Biosensor; Nanowires orientation; Reversible modulation