title: Identification of kidney tumor tissue by infrared spectroscopy of extracellular matrix.
abstract: Fourier transform infrared (FT-IR) spectroscopy was applied to characterize the extracellular matrix (ECM) of kidney tumor tissue and normal kidney tissue. Freshly resected tissue samples from 31 patients were pressed on a CaF₂ substrate. FT-IR spectra obtained from ECM of tumor tissue exhibit stronger absorption bands in the spectral region from 1000 to 1200 cm⁻¹ and around 1750 cm⁻¹ than those obtained from normal tissue. It is likely that the spectra of ECM of kidney tumor tissue with large increases in the intensities of these bands represent a higher concentration of fatty acids and glycerol. Amide I and amide II bands are stronger in the spectra of ECM from normal tissue, indicating a higher level of proteins. Our results suggest that FT-IR spectroscopy of the ECM is an innovative emerging technology for real-time intraoperative tumor diagnosis, which may improve margin clearance in renal cancer surgery.
authors.affiliation: Vilnius University, Department of General Physics and Spectroscopy, Sauletekio av. 9 bl. 3, Vilnius, LT-10234, Lithuania.
authors.forename: Vidita
authors.initials: V
authors.lastname: Urboniene
authors.affiliation: Vilnius University, Department of General Physics and Spectroscopy, Sauletekio av. 9 bl. 3, Vilnius, LT-10234, Lithuania.
authors.forename: Milda
authors.initials: M
authors.lastname: Pucetaite
authors.affiliation: Vilnius University, Urology Center, Santariskiu Str. 2, LT-08661 Vilnius, Lithuania.
authors.forename: Feliksas
authors.initials: F
authors.lastname: Jankevicius
authors.affiliation: Vilnius University, Urology Center, Santariskiu Str. 2, LT-08661 Vilnius, Lithuania.
authors.forename: Arunas
authors.initials: A
authors.lastname: Zelvys
authors.affiliation: Vilnius University, Department of General Physics and Spectroscopy, Sauletekio av. 9 bl. 3, Vilnius, LT-10234, Lithuania.
authors.forename: Valdas
authors.initials: V
authors.lastname: Sablinskas
authors.affiliation: Vilnius University, Department of General Physics and Spectroscopy, Sauletekio av. 9 bl. 3, Vilnius, LT-10234, LithuaniacTechnische Universität Dresden, Medizinische Fakultät Carl Gustav Carus, Klinisches Sensoring und Monitoring, Fetscher Str. 74, 01307.
authors.forename: Gerald
authors.initials: G
authors.lastname: Steiner
mesh_terms: D000465:Algorithms; D014408:Biomarkers, Tumor / Q000032:analysis*; D002292:Carcinoma, Renal Cell / Q000737:chemistry* / Q000175:diagnosis*; D003936:Diagnosis, Computer-Assisted / Q000379:methods; D005109:Extracellular Matrix / Q000737:chemistry*; D006801:Humans; D007680:Kidney Neoplasms / Q000737:chemistry* / Q000175:diagnosis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor