title: pH sensitive N-succinyl chitosan grafted polyacrylamide hydrogel for oral insulin delivery.
abstract: pH sensitive PAA/S-chitosan hydrogel was prepared using ammonium persulfate (APS) as an initiator and methylenebisacrylamide (MBA) as a crosslinker for oral insulin delivery. The synthesized copolymer was characterized by Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) study; morphology was observed under scanning electron microscope (SEM). The PAA/S-chitosan with ∼ 38% of insulin loading efficiency (LE) and ∼ 76% of insulin encapsulation efficiency (EE), showed excellent pH sensitivity, retaining ∼ 26% of encapsulated insulin in acidic stomach pH 1.2 and releasing of ∼ 98% of insulin in the intestine (pH 7.4), providing a prolonged attachment with the intestinal tissue. The oral administration of insulin loaded PAA/S-chitosan hydrogel was successful in lowering the blood glucose level of diabetic mice. The bioavailability of insulin was ∼ 4.43%. Furthermore, no lethality or toxicity was documented after its peroral administration. Thus, PAA/S-chitosan hydrogel could serve as a promising oral insulin carrier in future.
authors.affiliation: Department of Polymer Science and Technology, University of Calcutta, 92, A.P.C. Road, Kolkata 700009, India.
authors.forename: Piyasi
authors.initials: P
authors.lastname: Mukhopadhyay
authors.affiliation: Department of Polymer Science and Technology, University of Calcutta, 92, A.P.C. Road, Kolkata 700009, India.
authors.forename: Kishor
authors.initials: K
authors.lastname: Sarkar
authors.affiliation: Department of Physiology, University of Calcutta, 92, A.P.C. Road, Kolkata 700009, India.
authors.forename: Sourav
authors.initials: S
authors.lastname: Bhattacharya
authors.affiliation: Department of Polymer Science and Technology, University of Calcutta, 92, A.P.C. Road, Kolkata 700009, India.
authors.forename: Aditi
authors.initials: A
authors.lastname: Bhattacharyya
authors.affiliation: Department of Physiology, University of Calcutta, 92, A.P.C. Road, Kolkata 700009, India.
authors.forename: Roshnara
authors.initials: R
authors.lastname: Mishra
authors.affiliation: Department of Polymer Science and Technology, University of Calcutta, 92, A.P.C. Road, Kolkata 700009, India. Electronic address: ppk923@yahoo.com.
authors.forename: P P
authors.initials: PP
authors.lastname: Kundu
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D048271:Chitosan / Q000737:chemistry; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy; D004337:Drug Carriers / Q000008:administration & dosage* / Q000009:adverse effects / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000008:administration & dosage / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D008111:Liver Function Tests; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D018675:Toxicity Tests
chemical_list: D000180:Acrylic Resins; D004337:Drug Carriers; D007328:Insulin; C016680:polyacrylamide gels; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan
keywords: Hydrogel; Oral insulin delivery; S-chitosan; pH sensitivity