title: Down-regulated miR-15a mediates the epithelial-mesenchymal transition in renal tubular epithelial cells promoted by high glucose.
abstract: High glucose (HG) has been reported to be associated with renal dysfunction. And one potential mechanism underlining the dysfunction is the epithelial-mesenchymal transition (EMT) of renal tubular epithelial cells. Present study showed that EMT was induced in the HG-treated renal tubular epithelial cells by promoting the expression of mesenchymal phenotype molecules, such as α-SMA and collagen I, and down-regulating the expression of epithelial phenotype molecule E-cadherin. Moreover, we have identified the down-regulation of miR-15a which was accompanied with the HG-induced EMT. And the miR-15a overexpression inhibited the α-SMA, collagen I expression, and the promotion of E-cadherin expression by targeting and down-regulating AP4 which was also significantly promoted by the HG in the renal tubular epithelial cells. Thus, this study revealed that the weakening regulation on the AP4 expression by miR-15a might contribute to the HG-induced EMT in the renal tubular epithelial cells.
authors.affiliation: a Department of Nephrology , General Hospital of Daqing Oil Field, Daqing , China.
authors.forename: Tingli
authors.initials: T
authors.lastname: Sun
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Wenpeng
authors.initials: W
authors.lastname: Dong
authors.forename: Ruiyan
authors.initials: R
authors.lastname: Wang
authors.forename: Peilong
authors.initials: P
authors.lastname: Ma
authors.forename: Ping
authors.initials: P
authors.lastname: Kang
authors.forename: Hongbo
authors.initials: H
authors.lastname: Zhang
authors.forename: Changying
authors.initials: C
authors.lastname: Xie
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Lijie
authors.initials: L
authors.lastname: Zhao
mesh_terms: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000378:metabolism; D002460:Cell Line; D004268:DNA-Binding Proteins; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects*; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007684:Kidney Tubules / Q000166:cytology*; D035683:MicroRNAs / Q000235:genetics*; D016601:RNA-Binding Proteins
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; C505361:MIRN15 microRNA, human; D035683:MicroRNAs; C404766:REPIN1 protein, human; D016601:RNA-Binding Proteins; D005947:Glucose
keywords: AP4; epithelial–mesenchymal transition (EMT); high glucose; miR-15a; renal tubular epithelial cells