title: Maintenance of potential spermatogonial stem cells in vitro by GDNF treatment in a chondrichthyan model (Scyliorhinus canicula L.).
abstract: Previous work in dogfish, Scyliorhinus canicula, has identified the testicular germinative area as the spermatogonial stem cell niche. In the present study, an in vitro co-culture system of spermatogonia and somatic cells from the germinative area was developed. Long-term maintenance of spermatogonia has been successful, and addition of GDNF has promoted the development of clones of spermatogonia expressing stem cell characteristics such as alkaline phosphatase activity and has allowed maintenance of self-renewal in spermatogonia for at least 5 mo under culture conditions, notably by decreasing cell apoptosis. Furthermore, clones of spermatogonia expressed the receptor of GDNF, GFRalpha1, which is consistent with the effect of GDNF on cells despite the lack of identification of a GDNF sequence in the dogfish's transcriptome. However, a sequence homologous to artemin has been identified, and in silico analysis supports the hypothesis that artemin could replace GDNF in the germinative area in dogfish. This study, as the first report on long-term in vitro maintenance of spermatogonia in a chondrichthyan species, suggests that the GFRalpha1 signaling function in self-renewal of spermatogonial stem cells is probably conserved in gnathostomes.
authors.affiliation: Normandie University, Caen, France University of Caen Basse-Normandie, BOREA, Caen, France Centre National de la Recherche Scientifique, UMR 7208, Caen, France.
authors.forename: Aude
authors.initials: A
authors.lastname: Gautier
authors.affiliation: Normandie University, Caen, France University of Caen Basse-Normandie, BOREA, Caen, France Centre National de la Recherche Scientifique, UMR 7208, Caen, France Kelia, Group Cellis Pharma, Parc Technopolitain Atalante Saint Malo, Saint Malo, France.
authors.forename: Adrien
authors.initials: A
authors.lastname: Bosseboeuf
authors.affiliation: Kelia, Group Cellis Pharma, Parc Technopolitain Atalante Saint Malo, Saint Malo, France.
authors.forename: Pierrick
authors.initials: P
authors.lastname: Auvray
authors.affiliation: Normandie University, Caen, France University of Caen Basse-Normandie, BOREA, Caen, France Centre National de la Recherche Scientifique, UMR 7208, Caen, France pascal.sourdaine@unicaen.fr.
authors.forename: Pascal
authors.initials: P
authors.lastname: Sourdaine
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology* / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers; D018929:Cell Culture Techniques; D004284:Dogfish / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000494:pharmacology*; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors / Q000235:genetics / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D013093:Spermatogonia / Q000502:physiology*; D013737:Testis / Q000378:metabolism
chemical_list: D015415:Biomarkers; D029941:Fish Proteins; D051100:Glial Cell Line-Derived Neurotrophic Factor; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors; D009419:Nerve Tissue Proteins
keywords: GDNF; culture; dogfish; self-renewal; spermatogonial stem cells