title: A quantitative multiplexed mass spectrometry assay for studying the kinetic of residue-specific histone acetylation.
abstract: Histone acetylation is involved in gene regulation and, most importantly, aberrant regulation of histone acetylation is correlated with major human diseases. Although many lysine acetyltransferases (KATs) have been characterized as being capable of acetylating multiple lysine residues on histones, how different factors such as enzyme complexes or external stimuli (e.g. KAT activators or inhibitors) alter KAT specificity remains elusive. In order to comprehensively understand how the homeostasis of histone acetylation is maintained, a method that can quantitate acetylation levels of individual lysines on histones is needed. Here we demonstrate that our mass spectrometry (MS)-based method accomplishes this goal. In addition, the high throughput, high sensitivity, and high dynamic range of this method allows for effectively and accurately studying steady-state kinetics. Based on the kinetic parameters from in vitro enzymatic assays, we can determine the specificity and selectivity of a KAT and use this information to understand what factors influence histone acetylation. These approaches can be used to study the enzymatic mechanisms of histone acetylation as well as be adapted to other histone modifications. Understanding the post-translational modification of individual residues within the histones will provide a better picture of chromatin regulation in the cell.
authors.affiliation: Department of Cancer Biology, Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
authors.forename: Yin-Ming
authors.initials: YM
authors.lastname: Kuo
authors.affiliation: Department of Cancer Biology, Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
authors.forename: Ryan A
authors.initials: RA
authors.lastname: Henry
authors.affiliation: Department of Cancer Biology, Fox Chase Cancer Center, Philadelphia, PA 19111, USA. Electronic address: Andrew.Andrews@fccc.edu.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Andrews
mesh_terms: D000107:Acetylation; D002851:Chromatography, High Pressure Liquid; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D013058:Mass Spectrometry / Q000379:methods*; D011499:Protein Processing, Post-Translational
chemical_list: D006657:Histones
keywords: Acetylation; Enzyme kinetics; Histone; Mass spectrometry; Post-translational modification