title: Responses to ROS inducer agents in zebrafish cell line: differences between copper and UV-B radiation.
abstract: Fish are commonly exposed to environmental pollutants, which in turns could induce an oxidative stress. So, it is important to understand the effects and the responses elicited by these toxicants in fish species, being fish cell lines important tools for this purpose. Thus, the aim of the present study was to compare the effects of copper and UV-B radiation exposure on zebrafish hepatocytes (ZFL lineage) in terms of reactive oxygen species (ROS) levels, sulfhydril groups content and mRNA levels of important genes related to cellular response to toxic agents. Exposure of ZFL cells to UV-B radiation (23.3 mJ/cm(2)) significantly increased levels of intracellular ROS and mRNA of both superoxide dismutase isoforms (sod1 and sod2), three glutathione S-transferase isoforms (gstα, gstµ and gstπ) and a heat shock protein (hsp70). However, no changes in nonprotein sulfhydryl groups (NP-SH) content, as well as in the mRNA levels of genes related to glutathione (GSH) synthesis and recycling, were observed. Contrary to this, copper exposure (20 mg/L) diminished NP-SH content and increased the levels of mRNA of genes related to GSH synthesis (gclc and gs). Moreover, copper exposure increases the mRNA levels of some genes related to antioxidant defenses (gpx and gstπ), biotransformation reactions (cyp1a1) and protein repair (hsp70). In conclusion, these results demonstrated that both toxicants could increase ROS levels in ZFL cell line, but the responses are different, which could be related to activation of different signaling pathways.
authors.affiliation: Programa de Pós-Graduação em Ciências Fisiológicas - Fisiologia Animal Comparada, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália km 8, Campus Carreiros, Rio Grande, RS, CEP 96203-900, Brazil.
authors.forename: Regina Coimbra
authors.initials: RC
authors.lastname: Rola
authors.forename: Luis Fernando
authors.initials: LF
authors.lastname: Marins
authors.forename: Luiz Eduardo Maia
authors.initials: LE
authors.lastname: Nery
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: da Rosa
authors.forename: Juliana Zomer
authors.initials: JZ
authors.lastname: Sandrini
mesh_terms: D000818:Animals; D002460:Cell Line; D003300:Copper / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014466:Ultraviolet Rays / Q000009:adverse effects*; D015027:Zebrafish
chemical_list: D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D003300:Copper