title: Sensing Reversible Protein-Ligand Interactions with Single-Walled Carbon Nanotube Field-Effect Transistors.
abstract: We report on the reversible detection of CaptAvidin, a tyrosine modified avidin, with single-walled carbon nanotube (SWNT) field-effect transistors (FETs) noncovalently functionalized with biotin moieties using 1-pyrenebutyric acid as a linker. Binding affinities at different pH values were quantified, and the sensor's response at various ionic strengths was analyzed. Furthermore, protein "fingerprints" of NeutrAvidin and streptavidin were obtained by monitoring their adsorption at several pH values. Moreover, gold nanoparticle decorated SWNT FETs were functionalized with biotin using 1-pyrenebutyric acid as a linker for the CNT surface and (±)-α-lipoic acid linkers for the gold surface, and reversible CaptAvidin binding is shown, paving the way for potential dual mode measurements with the addition of surface enhanced Raman spectroscopy (SERS).
authors.affiliation: Institute for Nanoelectronics, Technische Universität München , Arcisstraße 21, 80333, Munich, Germany.
authors.forename: Alexandra M
authors.initials: AM
authors.lastname: Münzer
authors.affiliation: Department of Chemistry, University of Pittsburgh , Pittsburgh, Pennsylvania 15260, United States.
authors.forename: Wanji
authors.initials: W
authors.lastname: Seo
authors.affiliation: Department of Chemistry, University of Pittsburgh , Pittsburgh, Pennsylvania 15260, United States.
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Morgan
authors.affiliation: Department of Chemistry, University of Pittsburgh , Pittsburgh, Pennsylvania 15260, United States.
authors.forename: Zachary P
authors.initials: ZP
authors.lastname: Michael
authors.affiliation: Department of Chemistry, University of Pittsburgh , Pittsburgh, Pennsylvania 15260, United States.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute for Nanoelectronics, Technische Universität München , Arcisstraße 21, 80333, Munich, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Melzer
authors.affiliation: Institute for Nanoelectronics, Technische Universität München , Arcisstraße 21, 80333, Munich, Germany.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Scarpa
authors.affiliation: Department of Chemistry, University of Pittsburgh , Pittsburgh, Pennsylvania 15260, United States.
authors.forename: Alexander
authors.initials: A
authors.lastname: Star