title: Three-way interactions between mosquito population, viral strain and temperature underlying chikungunya virus transmission potential.
abstract: Interactions between pathogens and their insect vectors in nature are under the control of both genetic and non-genetic factors, yet most studies on mosquito vector competence for human pathogens are conducted in laboratory systems that do not consider genetic and/or environmental variability. Evaluating the risk of emergence of arthropod-borne viruses (arboviruses) of public health importance such as chikungunya virus (CHIKV) requires a more realistic appraisal of genetic and environmental contributions to vector competence. In particular, sources of variation do not necessarily act independently and may combine in the form of interactions. Here, we measured CHIKV transmission potential by the mosquito Aedes albopictus in all combinations of six worldwide vector populations, two virus strains and two ambient temperatures (20°C and 28°C). Overall, CHIKV transmission potential by Ae. albopictus strongly depended on the three-way combination of mosquito population, virus strain and temperature. Such genotype-by-genotype-by-environment (G × G × E) interactions question the relevance of vector competence studies conducted with a simpler set of conditions. Our results highlight the need to account for the complex interplay between vectors, pathogens and environmental factors to accurately assess the potential of vector-borne diseases to emerge.
authors.affiliation: Department of Virology, Institut Pasteur, Arboviruses and Insect Vectors Laboratory, Paris, France karima.zouache@pasteur.fr.
authors.forename: Karima
authors.initials: K
authors.lastname: Zouache
authors.affiliation: Department of Genomes and Genetics, Institut Pasteur-Centre National de la Recherche Scientifique URA 3012, Insect-Virus Interactions Group, Paris, France Institut de Recherche Biomédicale des Armées (IRBA), Unité d'Entomologie, Brétigny-sur-Orge, France.
authors.forename: Albin
authors.initials: A
authors.lastname: Fontaine
authors.affiliation: Department of Virology, Institut Pasteur, Arboviruses and Insect Vectors Laboratory, Paris, France Université Pierre et Marie Curie, Cellule Pasteur UPMC, Paris, France.
authors.forename: Anubis
authors.initials: A
authors.lastname: Vega-Rua
authors.affiliation: Department of Virology, Institut Pasteur, Arboviruses and Insect Vectors Laboratory, Paris, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Mousson
authors.affiliation: Institut Pasteur, Genotyping of Pathogens and Public Health, Paris, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Thiberge
authors.affiliation: Department of Virology, Institut Pasteur, Arboviruses and Insect Vectors Laboratory, Paris, France Laboratório de Transmissores de Hematozoários, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Lourenco-De-Oliveira
authors.affiliation: Institut Pasteur, Genotyping of Pathogens and Public Health, Paris, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Caro
authors.affiliation: Department of Genomes and Genetics, Institut Pasteur-Centre National de la Recherche Scientifique URA 3012, Insect-Virus Interactions Group, Paris, France.
authors.forename: Louis
authors.initials: L
authors.lastname: Lambrechts
authors.affiliation: Department of Virology, Institut Pasteur, Arboviruses and Insect Vectors Laboratory, Paris, France.
authors.forename: Anna-Bella
authors.initials: AB
authors.lastname: Failloux
mesh_terms: D000330:Aedes / Q000235:genetics* / Q000821:virology*; D018354:Alphavirus Infections / Q000635:transmission*; D000818:Animals; D002646:Chikungunya virus / Q000235:genetics*; D005838:Genotype; D007303:Insect Vectors / Q000235:genetics* / Q000821:virology*; D051379:Mice; D013696:Temperature
keywords: arbovirus; chikungunya; interaction; mosquito; temperature; transmission