title: Insecticidal activity and chemical composition of the Morinda lucida essential oil against pulse beetle Callosobruchus maculatus.
abstract: Insecticidal activity of essential oil extracted from Morinda lucida was tested on pulse beetle Callosobruchus maculatus, which is a pest that causes serious damage to several pulses. The insecticidal activity was compared with two pesticides, Phostoxin and Primo-ban-20. 120 mixed sex adult C. maculatus were introduced, along with 30 g of cowpeas. Four concentrations (0.40, 0.20, 0.10, and 0.05 μg/mL) of the M. lucida essential oil, Phostoxin, and Primo-ban-20 were tested. Essential oil chemical composition was analyzed by GC-MS. M. lucida essential oil showed a high toxicological effect, producing 100% mortality after 72 hours at a dose of 0.20 μg/mL. M. lucida essential oil had a potent insecticidal activity (LC90 = 0.629 μg/mL) compared to both pesticides, Phostoxin (LC90 = 0.652 μg/mL) and Primo-ban-20 (LC90 = 0.726 μg/mL), at 24 h. The main compounds of the essential oil were the oxygenated monoterpenoids, 1,8-cineole (43.4%), and α-terpinyl acetate (14.5%), and the monoterpene hydrocarbons, mostly sabinene (8.2%) and β-pinene (4.0%). Results clearly indicate that M. lucida essential oil can be used as an effective alternative for pulse beetle C. maculatus control, and it could be tested against other pulse beetles affecting Asia and Africa and throughout the world, thereby reducing use of synthetic pesticides.
authors.affiliation: Department of Chemistry, Lagos State University, PMB 0001, Lasu Post Office, Ojo, Lagos 102001, Nigeria.
authors.forename: Moses S
authors.initials: MS
authors.lastname: Owolabi
authors.affiliation: Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Avenida Normalistas 800, Col. Colinas de la Normal, 44270 Guadalajara, JAL, Mexico.
authors.forename: Eduardo
authors.identifier: 0000-0003-4783-841X
authors.initials: E
authors.lastname: Padilla-Camberos
authors.affiliation: Department of Chemistry, Lagos State University, PMB 0001, Lasu Post Office, Ojo, Lagos 102001, Nigeria.
authors.forename: Akintayo L
authors.initials: AL
authors.lastname: Ogundajo
authors.affiliation: Department of Chemistry, Lagos State University, PMB 0001, Lasu Post Office, Ojo, Lagos 102001, Nigeria.
authors.forename: Isiaka A
authors.initials: IA
authors.lastname: Ogunwande
authors.affiliation: Dipartimento di Chimica Bioorganica e Biofarmacia, Universita di Pisa, Via Bonanno 33, 56126 Pisa, Italy.
authors.forename: Guido
authors.identifier: 0000-0003-2418-9349
authors.initials: G
authors.lastname: Flamini
authors.affiliation: Department of Chemistry, Lagos State University, PMB 0001, Lasu Post Office, Ojo, Lagos 102001, Nigeria.
authors.forename: Olaniyi K
authors.initials: OK
authors.lastname: Yusuff
authors.affiliation: Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Avenida Normalistas 800, Col. Colinas de la Normal, 44270 Guadalajara, JAL, Mexico.
authors.forename: Kirk
authors.identifier: 0000-0002-5962-1704
authors.initials: K
authors.lastname: Allen
authors.affiliation: Ingeniería en Biotecnología, Universidad Politécnica del Valle de Toluca, Carretera Toluca-Almoloya de Juárez km 5.6, Camino la Loma, Santiaguito Tlalcilalcali, 50900 Almoloya de Juárez, Mexico.
authors.forename: Karen Isabel
authors.initials: KI
authors.lastname: Flores-Fernandez
authors.affiliation: Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Avenida Normalistas 800, Col. Colinas de la Normal, 44270 Guadalajara, JAL, Mexico.
authors.forename: Jose Miguel
authors.initials: JM
authors.lastname: Flores-Fernandez
mesh_terms: D000818:Animals; D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds / Q000737:chemistry; D001517:Coleoptera / Q000187:drug effects*; D003511:Cyclohexanols / Q000737:chemistry; D000077591:Eucalyptol; D008401:Gas Chromatography-Mass Spectrometry; D007306:Insecticides / Q000737:chemistry* / Q000494:pharmacology*; D039821:Monoterpenes / Q000737:chemistry; D032066:Morinda / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds; D003511:Cyclohexanols; D007306:Insecticides; D039821:Monoterpenes; D009822:Oils, Volatile; C010789:beta-pinene; C035127:sabinene; D000077591:Eucalyptol