title: Ultrasensitive electrochemical immunoassay for CEA through host-guest interaction of β-cyclodextrin functionalized graphene and Cu@Ag core-shell nanoparticles with adamantine-modified antibody.
abstract: A novel non-enzymatic immunoassay was designed for ultrasensitive electrochemical detection of carcino-embryonic antigen (CEA) using β-cyclodextrin functionalized Cu@Ag (Cu@Ag-CD) core-shell nanoparticles as labels and β-cyclodextrin functionalized graphene nanosheet (CD-GN) as sensor platform. CD-GN has excellent conductivity which promoted the electric transmission between base solution and electrode surface and enhanced sensitivity of immunosensor. In addition, owing to supramolecular recognition of CD-GN for the guest molecule, quite a few synthesized adamantine-modified primary antibodies (ADA-Ab1) were immobilized on the CD-GN by supramolecular host-guest interaction between CD and ADA. Cu@Ag-CD as a signal tag could be captured by ADA-modified secondary antibody (ADA-Ab2) through a host-guest interaction, leading to a large loading of Cu@Ag nanoparticles with high electrical conductivity and catalytic activity. The fabricated immunosensor exhibits excellent analytical performance for the measurement of CEA with wide range linear (0.0001-20 ng/mL), low detection limit (20 fg/mL), good sensitivity, reproducibility and stability, which provide an enormous application prospect in clinical diagnostics.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Zhankui
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Fengjie
authors.initials: F
authors.lastname: Su
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Gao
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Pang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China. Electronic address: sdjndxwq@163.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation; D002272:Carcinoembryonic Antigen / Q000032:analysis* / Q000276:immunology; D003217:Conductometry / Q000295:instrumentation*; D003300:Copper / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D007118:Immunoassay / Q000295:instrumentation*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008832:Microchemistry / Q000295:instrumentation; D025941:Protein Interaction Mapping / Q000295:instrumentation; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000906:Antibodies; D002272:Carcinoembryonic Antigen; D047392:beta-Cyclodextrins; D006046:Gold; D006108:Graphite; D003300:Copper; D000537:Aluminum Oxide
keywords: Adamantine-modified antibody; Host–guest interaction; Immunosensor; β-cyclodextrin functionalized Cu@Ag nanoparticles; β-cyclodextrin functionalized graphene