title: Structural analysis of cerebrosides from Aspergillus fungi: the existence of galactosylceramide in A. oryzae.
abstract: Glucosylceramide and galactosylceramide were detected in three Aspergillus species: Aspergillus oryzae, Aspergillus sojae and Aspergillus. awamori, using borate-coated TLC. The cerebrosides from A. oryzae were further purified by ion exchange and iatrobeads column chromatographies with or without borate, and determined the composition of sugar, fatty acid and sphingoid base by GC/MS, MALDI-TOF/MS and (1)H-NMR. We identified them as β-glucosylceramide and β-galactosylceramide. The ceramide moiety of both cerebrosides consisted mainly of 2-hydroxystearic acid and either 9-methyl-octadeca-4, 8-sphingadienine or octadeca-4, 8-sphingadienine. To our knowledge, this is the first study to provide evidence for the presence of β-galactosylceramide in A. oryzae.
authors.affiliation: Graduate School of Biostudies, Kyoto University, Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Tani
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Amaishi
authors.forename: Tori
authors.initials: T
authors.lastname: Funatsu
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ito
authors.forename: Saki
authors.initials: S
authors.lastname: Itonori
authors.forename: Yoji
authors.initials: Y
authors.lastname: Hata
authors.forename: Hisashi
authors.initials: H
authors.lastname: Ashida
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D001236:Aspergillus oryzae / Q000737:chemistry*; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D005699:Galactosylceramides / Q000032:analysis* / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D005963:Glucosylceramides / Q000032:analysis / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D005699:Galactosylceramides; D005963:Glucosylceramides