title: Automatic generic registration of mass spectrometry imaging data to histology using nonlinear stochastic embedding.
abstract: The combination of mass spectrometry imaging and histology has proven a powerful approach for obtaining molecular signatures from specific cells/tissues of interest, whether to identify biomolecular changes associated with specific histopathological entities or to determine the amount of a drug in specific organs/compartments. Currently there is no software that is able to explicitly register mass spectrometry imaging data spanning different ionization techniques or mass analyzers. Accordingly, the full capabilities of mass spectrometry imaging are at present underexploited. Here we present a fully automated generic approach for registering mass spectrometry imaging data to histology and demonstrate its capabilities for multiple mass analyzers, multiple ionization sources, and multiple tissue types.
authors.affiliation: Division of Image Processing, Department of Radiology, ‡Center for Proteomics and Metabolomics, §Department of Human Genetics, ∥Department of Neurology, and ⊥Department of Pathology, Leiden University Medical Center , 2333 ZA Leiden, The Netherlands.
authors.forename: Walid M
authors.initials: WM
authors.lastname: Abdelmoula
authors.forename: Karolina
authors.initials: K
authors.lastname: Škrášková
authors.forename: Benjamin
authors.initials: B
authors.lastname: Balluff
authors.forename: Ricardo J
authors.initials: RJ
authors.lastname: Carreira
authors.forename: Else A
authors.initials: EA
authors.lastname: Tolner
authors.forename: Boudewijn P F
authors.initials: BP
authors.lastname: Lelieveldt
authors.forename: Laurens
authors.initials: L
authors.lastname: van der Maaten
authors.forename: Hans
authors.initials: H
authors.lastname: Morreau
authors.forename: Arn M J M
authors.initials: AM
authors.lastname: van den Maagdenberg
authors.forename: Ron M A
authors.initials: RM
authors.lastname: Heeren
authors.forename: Liam A
authors.initials: LA
authors.lastname: McDonnell
authors.forename: Jouke
authors.initials: J
authors.lastname: Dijkstra
mesh_terms: D000465:Algorithms; D000818:Animals; D001921:Brain / Q000033:anatomy & histology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D051379:Mice; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013964:Thyroid Neoplasms / Q000473:pathology