title: Enhanced succinic acid production and magnesium utilization by overexpression of magnesium transporter mgtA in Escherichia coli mutant.
abstract: In this study, a novel engineered Escherichia coli strain KMG111 was constructed by overexpression of mgtA in E. coli mutant DC1515. By adopting KMG111, nearly a concentration of succinic acid (32.41gL(-1)) with a yield of 0.81gg(-1) glucose, could be obtained in a batch fermentation by using the low-cost mixture of Mg(OH)2 and NH3·H2O to replace MgCO3 as the alkaline neutralizer. Moreover, the effect of the inhibitory compounds in lignocellulosic hydrolyzates on cell growth and succinic acid production could be relieved. In a 3-L bioreactor, the overall productivity and yield of succinic acid in the whole anaerobic stage were 2.15gL(-1)h(-1) and 0.86gg(-1) total sugar, respectively. This study was the first to report decreased alkaline neutralizer cost via genetic manipulation for succinic acid production, which contributed to the industrialization of this microbial synthesis process.
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Bioengineering, Chongqing University, Chongqing 400044, China.
authors.forename: Baoyun
authors.initials: B
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China.
authors.forename: Honghui
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Zhao
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China.
authors.forename: Lichun
authors.initials: L
authors.lastname: Dong
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China. Electronic address: danwang088@gmail.com.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D019149:Bioreactors; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering / Q000379:methods; D007218:Industrial Microbiology / Q000379:methods; D008031:Lignin; D008274:Magnesium / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; C036909:lignocellulose; D008031:Lignin; D019802:Succinic Acid; D000251:Adenosine Triphosphatases; C490561:MgtA protein, E coli; D008274:Magnesium
keywords: Alkaline neutralizer; Biomass hydrolysate; Escherichia coli; Magnesium transporter; Succinic acid