title: Paired related homeobox protein-like 1 (Prrxl1) controls its own expression by a transcriptional autorepression mechanism.
abstract: The homeodomain factor paired related homeobox protein-like 1 (Prrxl1) is crucial for proper assembly of dorsal root ganglia (DRG)-dorsal spinal cord (SC) pain-sensing circuit. By performing chromatin immunoprecipitation with either embryonic DRG or dorsal SC, we identified two evolutionarily conserved regions (i.e. proximal promoter and intron 4) of Prrxl1 locus that show tissue-specific binding of Prrxl1. Transcriptional assays confirm the identified regions can mediate repression by Prrxl1, while gain-of-function studies in Prrxl1 expressing ND7/23 cells indicate Prrxl1 can down-regulate its own expression. Altogether, our results suggest that Prrxl1 uses distinct regulatory regions to repress its own expression in DRG and dorsal SC.
authors.affiliation: Departamento de Biologia Experimental, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal; Morfofisiologia do Sistema Somatosensitivo, IBMC - Instituto de Biologia Celular e Molecular, 4150-180 Porto, Portugal. Electronic address: cesar48.48@gmail.com.
authors.forename: César B
authors.initials: CB
authors.lastname: Monteiro
authors.affiliation: Departamento de Biologia Experimental, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal; Morfofisiologia do Sistema Somatosensitivo, IBMC - Instituto de Biologia Celular e Molecular, 4150-180 Porto, Portugal. Electronic address: marianafacosta@gmail.com.
authors.forename: Mariana F
authors.initials: MF
authors.lastname: Costa
authors.affiliation: Departamento de Biologia Experimental, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal; Morfofisiologia do Sistema Somatosensitivo, IBMC - Instituto de Biologia Celular e Molecular, 4150-180 Porto, Portugal. Electronic address: cregueng@med.up.pt.
authors.forename: Carlos
authors.initials: C
authors.lastname: Reguenga
authors.affiliation: Departamento de Biologia Experimental, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal; Morfofisiologia do Sistema Somatosensitivo, IBMC - Instituto de Biologia Celular e Molecular, 4150-180 Porto, Portugal. Electronic address: limad@med.up.pt.
authors.forename: Deolinda
authors.initials: D
authors.lastname: Lima
authors.affiliation: Molecular Neurobiology, IGC - Instituto Gulbenkian de Ciência, 2780-156 Oeiras, Portugal. Electronic address: dscastro@igc.gulbenkian.pt.
authors.forename: Diogo S
authors.initials: DS
authors.lastname: Castro
authors.affiliation: Departamento de Biologia Experimental, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal; Morfofisiologia do Sistema Somatosensitivo, IBMC - Instituto de Biologia Celular e Molecular, 4150-180 Porto, Portugal. Electronic address: filipem@med.up.pt.
authors.forename: Filipe A
authors.initials: FA
authors.lastname: Monteiro
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D025461:Feedback, Physiological; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology; D020868:Gene Silencing; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; C433766:Prrxl1 protein, mouse; D014157:Transcription Factors
keywords: Autorepression; Chromatin immunoprecipitation; Dorsal root ganglia; Dorsal spinal cord; Paired related homeobox protein-like 1