title: A significant inhibitory effect on advanced glycation end product formation by catechin as the major metabolite of lotus seedpod oligomeric procyanidins.
abstract: Several lines of evidence suggested that B-type procyanidin oligomers from lotus seedpod (LSOPC) may effectively modulate the formation of advanced glycation end products (AGEs). In vivo, LSOPC is metabolized by intestinal flora to become various kinds of phenolic compounds that possess potent antioxidant activities. However, few reports of the absorption and metabolism of LSOPC have been revealed. In the present study, rats were orally administered with LSOPC at a dose of 300 mg/kg body weight. The metabolites of LSOPC in urine were elucidated by HPLC-MS/MS analysis 24 h post-administration. Eight major metabolites were significantly increased by the administration of 300 mg/kg of LSOPC (p < 0.01). The anti-glycative activity of LSOPC and its metabolites were investigated. The results showed that LSOPC and catechin had greater anti-glycative activities than other metabolites, which were positively correlated to their carbonyl scavenging activities and antioxidant capacities.
authors.affiliation: Natural Product Laboratory, Department of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. qianwu@umass.edu.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wu
authors.affiliation: College of food science and engineering, Wuhan Polytechnic University, Wuhan, Hubei 430070, China. lishuyisz@sina.com.
authors.forename: Shuyi
authors.initials: S
authors.lastname: Li
authors.affiliation: Natural Product Laboratory, Department of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. 15392909677@189.cn.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Food and Biology Science and Technology, Chutian College, Huazhong Agricultural University, Wuhan, Hubei 430070, China. fuxiaoyan001@163.com.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Fu
authors.affiliation: Natural Product Laboratory, Department of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. suiyong0429@126.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Sui
authors.affiliation: Natural Product Laboratory, Department of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. gtt693532@126.com.
authors.forename: Tingting
authors.initials: T
authors.lastname: Guo
authors.affiliation: Natural Product Laboratory, Department of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. bijunxie@sina.com.
authors.forename: Bijun
authors.initials: B
authors.lastname: Xie
authors.affiliation: Natural Product Laboratory, Department of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. sunzhida@sina.com.
authors.forename: Zhida
authors.initials: Z
authors.lastname: Sun
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D044946:Biflavonoids / Q000494:pharmacology* / Q000652:urine; D002392:Catechin / Q000494:pharmacology* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors / Q000378:metabolism*; D020128:Inhibitory Concentration 50; D000070116:Lotus / Q000737:chemistry*; D008297:Male; D010636:Phenols / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000494:pharmacology* / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D044946:Biflavonoids; D017127:Glycation End Products, Advanced; D010636:Phenols; D010936:Plant Extracts; D044945:Proanthocyanidins; C017674:procyanidin; D002392:Catechin