title: Allelic variants at codon 146 in the PRNP gene show significant differences in the risk for natural scrapie in Cypriot goats.
abstract: Previous studies have shown the association between the polymorphisms serine (S) or aspartic acid (D) at codon 146 of the PRNP gene and resistance to scrapie. All goats aged >12 months (a total of 1075 animals) from four herds with the highest prevalence of scrapie in the country were culled and tested, of which 234 (21·7%) were positive by either the rapid test or immunohistochemistry (IHC) for any of the tissues tested. The odds of scrapie infection occurring in NN146 goats was 101 [95% credible interval (CrI) 19-2938] times higher than for non-NN146 or unknown genotypes. IHC applied to lymphoreticular tissue produced the highest sensitivity (94%, 95% CrI 90-97). The presence of putatively resistant non-NN146 alleles in the Cypriot goat population, severely affected by scrapie, provides a potential tool to reduce/eradicate scrapie provided that coordinated nationwide breeding programmes are implemented and maintained over time.
authors.affiliation: Animal Health and Veterinary Laboratories Agency,New Haw,Addlestone,Surrey,UK.
authors.forename: A
authors.initials: A
authors.lastname: Ortiz-Pelaez
authors.affiliation: Veterinary Services,Nicosia,Cyprus.
authors.forename: S
authors.initials: S
authors.lastname: Georgiadou
authors.affiliation: Animal Health and Veterinary Laboratories Agency,New Haw,Addlestone,Surrey,UK.
authors.forename: M M
authors.initials: MM
authors.lastname: Simmons
authors.affiliation: Animal Health and Veterinary Laboratories Agency,New Haw,Addlestone,Surrey,UK.
authors.forename: O
authors.initials: O
authors.lastname: Windl
authors.affiliation: Animal Health and Veterinary Laboratories Agency,New Haw,Addlestone,Surrey,UK.
authors.forename: M
authors.initials: M
authors.lastname: Dawson
authors.affiliation: Animal Health and Veterinary Laboratories Agency,New Haw,Addlestone,Surrey,UK.
authors.forename: M E
authors.initials: ME
authors.lastname: Arnold
authors.affiliation: Veterinary Services,Nicosia,Cyprus.
authors.forename: P
authors.initials: P
authors.lastname: Neocleous
authors.affiliation: Veterinary Services,Nicosia,Cyprus.
authors.forename: P
authors.initials: P
authors.lastname: Papasavva-Stylianou
mesh_terms: D000818:Animals; D003062:Codon / Q000235:genetics; D003535:Cyprus / Q000453:epidemiology; D004198:Disease Susceptibility / Q000662:veterinary; D005260:Female; D015511:Goat Diseases / Q000453:epidemiology*; D006041:Goats; D008297:Male; D011110:Polymorphism, Genetic / Q000235:genetics; D018620:PrPSc Proteins / Q000235:genetics*; D015995:Prevalence; D012608:Scrapie / Q000453:epidemiology*
chemical_list: D003062:Codon; D018620:PrPSc Proteins
keywords: scrapie