title: Effect of Jianpi Bushen prescription on the expression of SHP-1 and apoptosis-related genes in chemically damaged model mice.
abstract: We investigated the effect of Jianpi Bushen prescription (JBP) on the expression of the SHP-1 and apoptosis-related genes in chemically damaged model mice and a compound e-jiao slurry (EJS) group (positive control). Kunming mice received an abdominal injection of 100 mg/kg cyclophosphamide once a day for 3 consecutive days to induce chemical damage. The mice underwent lavage at a suspension of 0.1 g/kg low-dose JBP (100%), high-dose JBP (200%), and 0.2 mL/10 g EJS twice a day for 9 days. mRNA and protein expression of SHP-1 in bone marrow mononuclear cells was detected using real-time polymerase chain reaction and Western blot; mRNA expression of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X (Bax) protein was detected by in situ hybridization. Expression of SHP-1 and Bax mRNA was significantly upregulated in the model group compared to the control group (P < 0.05). Expression in the low-dose JBP, high-dose JBP, and EJS groups was significantly downregulated compared with the model group (P < 0.05). The low-dose JBP group exhibited much lower SHP-1 and Bax mRNA expression levels. Compared with controls, Bcl-2 mRNA expression was significantly reduced in the model group (P < 0.05). Expression in the low-dose JBP, high-dose JBP, and EJS groups significantly increased compared with the model group (P < 0.05). The low-dose JBP group showed much higher Bcl-2 mRNA expression. Therefore, JBP regulates the expression of the SHP- 1, Bax, and Bcl-2 genes in chemically damaged mice.
authors.affiliation: Department of Integrated Chinese and Western Medicine, Wuhan General Hospital of Guangzhou Military Region, Wuhan, Hubei, China guilianshang@126.com.
authors.forename: D C
authors.initials: DC
authors.lastname: He
authors.affiliation: Department of Integrated Chinese and Western Medicine, Wuhan General Hospital of Guangzhou Military Region, Wuhan, Hubei, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Xiao
authors.affiliation: Department of Integrated Chinese and Western Medicine, Wuhan General Hospital of Guangzhou Military Region, Wuhan, Hubei, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Integrated Chinese and Western Medicine, Wuhan General Hospital of Guangzhou Military Region, Wuhan, Hubei, China.
authors.forename: H
authors.initials: H
authors.lastname: Lin
authors.affiliation: Department of Integrated Chinese and Western Medicine, Wuhan General Hospital of Guangzhou Military Region, Wuhan, Hubei, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Ding
authors.affiliation: Department of Integrated Chinese and Western Medicine, Wuhan General Hospital of Guangzhou Military Region, Wuhan, Hubei, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Tu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000009:adverse effects; D015536:Down-Regulation; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D008297:Male; D051379:Mice; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6 / Q000235:genetics* / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D003520:Cyclophosphamide; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6; C509248:Ptpn6 protein, mouse