title: Der p 2 can induce bystander activation of B cells derived from patients with systemic lupus erythematosus.
abstract: Although many patients with SLE also have allergies, the immunological events triggering the onset and progression of the clinical manifestations of SLE by allergens have yet to be clarified. A total of three autoantigens, phosphoglycerate kinase 1 (PGK-1), triosephosphate isomerase (TIM) and enolase were identified by autologous serum in B cell lysate derived from HDM allergic SLE patients after Der p 2 stimulation. Autoantigen, TRIM-21 expression were also significantly increased in B cells derived from HDM allergic SLE patients. In PBMCs derived from SLE patients, the concentration of anti-PGK-1 was significantly upregulated after Der p 2 stimulation compared to HDM allergic without SLE patients and healthy subjects. Inflammatory related cytokines and chemokines include IL-1β, IL-6, IL-8, CXCL5 could be upregulated after Der p 2 stimulation in PBMCs derived from HDM allergic SLE patients. In conclusion, our data demonstrated that long-term allergen exposure could be a contributing factor in the development of SLE.
authors.affiliation: Institute of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan.
authors.forename: Sheng-Jie
authors.initials: SJ
authors.lastname: Yu
authors.affiliation: Center for Translational Medicine, Department of Medical Research, Taichung Veterans General Hospital; Department of BioIndustry Technology, Da Yeh University, Changhua, Taiwan; Department of Medical Technology, Jen Ten College of Medicine, Nursing and Management, Miaoli, Taiwan.
authors.forename: En-Chih
authors.initials: EC
authors.lastname: Liao
authors.affiliation: Institute of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan; Section of immunology and rheumatology, Department of Internal Medicine, Taichung Veterans General Hospital; Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan. Electronic address: jawji@vghtc.gov.tw.
authors.forename: Jaw-Ji
authors.initials: JJ
authors.lastname: Tsai
mesh_terms: D000485:Allergens / Q000276:immunology; D000595:Amino Acid Sequence; D000917:Antibody Formation; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D060829:Arthropod Proteins / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology; D001324:Autoantigens / Q000737:chemistry / Q000276:immunology; D015551:Autoimmunity / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D018836:Inflammation Mediators / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology*; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D001323:Autoantibodies; D001324:Autoantigens; D016207:Cytokines; C451163:Dermatophagoides pteronyssinus antigen p 2; D018836:Inflammation Mediators; D007073:Immunoglobulin E
keywords: Allergy; Autoantibody; Autoantigen; HDM; SLE