title: B7-H3 expression in colorectal cancer: associations with clinicopathological parameters and patient outcome.
abstract: BACKGROUND
  We have previously reported overexpression of the immunoregulatory protein B7-H3 in colorectal cancer and that nuclear expression predicted poor outcome in colon cancer patients. The present study was performed to examine the prognostic role of B7-H3 in an independent colorectal cancer cohort.
  
  
  METHODS
  Using tissue microarrays from 731 colorectal cancer patients, tumour B7-H3 expression was assessed by immunohistochemistry. Associations with clinicopathological parameters and patient outcome were investigated.
  
  
  RESULTS
  Nuclear expression of B7-H3 in cancer cells was present in 27% of the samples in the total study cohort, while cytoplasmic/membrane and stromal expression was seen in 86% and 77% of the samples, respectively. Nuclear B7-H3 had no prognostic relevance in the complete outcome cohort, neither in colon cancer patients. However, nuclear B7-H3 was significantly associated with reduced recurrence-free survival in TNM stage I colorectal cancer patients.
  
  
  CONCLUSIONS
  Overexpression of B7-H3 in colorectal cancer was confirmed, but in contrast to previous results, nuclear B7-H3 was not a strong prognostic biomarker in this cohort. The discrepancy might be related to the use of single-core tissue microarrays for detection of the heterogeneously expressed B7-H3, and the role of B7-H3 in colorectal cancer still needs further examination.
authors.affiliation: Department of Tumor Biology, Norwegian Radium Hospital, Oslo University Hospital, PO Box 4950, Nydalen, N-0424 Oslo, Norway. vibing@rr-research.no.
authors.forename: Vibeke A
authors.initials: VA
authors.lastname: Ingebrigtsen
authors.forename: Kjetil
authors.initials: K
authors.lastname: Boye
authors.forename: Jahn M
authors.initials: JM
authors.lastname: Nesland
authors.forename: Arild
authors.initials: A
authors.lastname: Nesbakken
authors.forename: Kjersti
authors.initials: K
authors.lastname: Flatmark
authors.forename: Øystein
authors.initials: Ø
authors.lastname: Fodstad
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D060887:B7 Antigens / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000401:mortality* / Q000473:pathology*; D003593:Cytoplasm / Q000378:metabolism; D018572:Disease-Free Survival; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D046888:Tissue Array Analysis
chemical_list: D060887:B7 Antigens; C423237:CD276 protein, human