title: Serotonergic neurons of the Drosophila air-puff-stimulated flight circuit.
abstract: Monoaminergic modulation of insect flight is well documented. Recently, we demonstrated that synaptic activity is required in serotonergic neurons for Drosophila flight. This requirement is during early pupal development, when the flight circuit is formed, as well as in adults. Using a Ca2+-activity-based GFP reporter, here we show that serotonergic neurons in both prothoracic and mesothoracic segments are activated upon air-puff-stimulated flight. Moreover ectopic activation of the entire serotonergic system by TrpA1, a heat activated cation channel, induces flight, even in the absence of an air-puff stimulus.
authors.affiliation: National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bellary Road, Bangalore, India.
authors.forename: Sufia
authors.initials: S
authors.lastname: Sadaf
authors.forename: Gaiti
authors.initials: G
authors.lastname: Hasan
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000378:metabolism; D002118:Calcium; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism; D005260:Female; D005426:Flight, Animal / Q000502:physiology*; D049452:Green Fluorescent Proteins; D007150:Immunohistochemistry; D007473:Ion Channels; D008297:Male; D059326:Serotonergic Neurons / Q000502:physiology*; D013569:Synapses / Q000502:physiology*; D000074025:TRPA1 Cation Channel; D050052:TRPC Cation Channels / Q000378:metabolism; D013696:Temperature
chemical_list: D029721:Drosophila Proteins; D007473:Ion Channels; D000074025:TRPA1 Cation Channel; D050052:TRPC Cation Channels; C475684:TrpA1 protein, Drosophila; D049452:Green Fluorescent Proteins; D002118:Calcium