title: Engineered M13 bacteriophage nanocarriers for intracellular delivery of exogenous proteins to human prostate cancer cells.
abstract: The size, well-defined structure, and relatively high folding energies of most proteins allow them to recognize disease-relevant receptors that present a challenge to small molecule reagents. While multiple challenges must be overcome in order to fully exploit the use of protein reagents in basic research and medicine, perhaps the greatest challenge is their intracellular delivery to a particular diseased cell. Here, we describe the genetic and enzymatic manipulation of prostate cancer cell-penetrating M13 bacteriophage to generate nanocarriers for the intracellular delivery of functional exogenous proteins to a human prostate cancer cell line.
authors.affiliation: Department of Chemistry, and ‡Department of Biochemistry & Molecular Biology, Colorado State University , Fort Collins, Colorado 80523, United States.
authors.forename: Sandra M
authors.initials: SM
authors.lastname: DePorter
authors.forename: Brian R
authors.initials: BR
authors.lastname: McNaughton
mesh_terms: D000595:Amino Acid Sequence; D017104:Bacteriophage M13 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D005818:Genetic Engineering; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D049329:Nanostructures; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D036022:Capsid Proteins; D011506:Proteins