title: The study of fetal rat model of intra-amniotic isoproterenol injection induced heart dysfunction and phenotypic switch of contractile proteins.
abstract: To establish a reliable isoproterenol induced heart dysfunction fetal rat model and understand the switches of contractile proteins, 45 pregnant rats were divided into 15 mg/kg-once, 15 mg/kg-twice, sham-operated once, sham-operated twice, and control groups. And 18 adult rats were divided into isoproterenol-treated and control groups. H&E staining, Masson staining, and transmission electron microscope were performed. Apoptotic rate assessed by TUNEL analysis and expressions of ANP, BNP, MMP-2, and CTGF of hearts were measured. Intra-amniotic injections of isoproterenol were supplied on E14.5 and E15.5 for fetuses and 7-day continuous intraperitoneal injections were performed for adults. Then echocardiography was performed with M-mode view assessment on E18.5 and 6 weeks later, respectively. Isoproterenol twice treated fetuses exhibited significant changes in histological evaluation, and mitochondrial damages were significantly severe with increased apoptotic rate. ANP and BNP increased and that of MMP-2 increased in isoproterenol twice treated group compared to control group, without CTGF. The isoforms transition of troponin I and myosin heavy chain of fetal heart dysfunction were opposite to adult procedure. The administration of intra-amniotic isoproterenol to fetal rats could induce heart dysfunction and the regulation of contractile proteins of fetuses was different from adult procedure.
authors.affiliation: Department of Pediatric Cardiovascular Disease, West China Second University Hospital, Sichuan University, No. 20, 3rd Section, South Renmin Road, Chengdu, Sichuan 610041, China.
authors.forename: Yifei
authors.identifier: 0000-0002-3096-4287
authors.initials: Y
authors.lastname: Li
authors.affiliation: West China Stomatology School, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Jie
authors.identifier: 0000-0003-0665-6935
authors.initials: J
authors.lastname: Fang
authors.affiliation: Department of Pediatric Cardiovascular Disease, West China Second University Hospital, Sichuan University, No. 20, 3rd Section, South Renmin Road, Chengdu, Sichuan 610041, China ; Ministry of Education Key Laboratory of Women and Children's Diseases and Birth Defects, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Yimin
authors.identifier: 0000-0001-9331-7653
authors.initials: Y
authors.lastname: Hua
authors.affiliation: Department of Pediatric Cardiovascular Disease, West China Second University Hospital, Sichuan University, No. 20, 3rd Section, South Renmin Road, Chengdu, Sichuan 610041, China ; Ministry of Education Key Laboratory of Women and Children's Diseases and Birth Defects, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Chuan
authors.identifier: 0000-0001-5165-8273
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Pediatric Cardiovascular Disease, West China Second University Hospital, Sichuan University, No. 20, 3rd Section, South Renmin Road, Chengdu, Sichuan 610041, China ; Ministry of Education Key Laboratory of Women and Children's Diseases and Birth Defects, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Dezhi
authors.initials: D
authors.lastname: Mu
authors.affiliation: Department of Pediatric Cardiovascular Disease, West China Second University Hospital, Sichuan University, No. 20, 3rd Section, South Renmin Road, Chengdu, Sichuan 610041, China ; Ministry of Education Key Laboratory of Women and Children's Diseases and Birth Defects, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Kaiyu
authors.identifier: 0000-0002-0347-6317
authors.initials: K
authors.lastname: Zhou
mesh_terms: D000318:Adrenergic beta-Agonists / Q000009:adverse effects* / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis; D004195:Disease Models, Animal; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006330:Heart Defects, Congenital / Q000139:chemically induced / Q000196:embryology / Q000401:mortality / Q000503:physiopathology; D007545:Isoproterenol / Q000009:adverse effects* / Q000494:pharmacology; D009124:Muscle Proteins / Q000096:biosynthesis*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000318:Adrenergic beta-Agonists; D009124:Muscle Proteins; D007545:Isoproterenol