title: Atypical cell populations associated with acquired resistance to cytostatics and cancer stem cell features: the role of mitochondria in nuclear encapsulation.
abstract: Until recently, acquired resistance to cytostatics had mostly been attributed to biochemical mechanisms such as decreased intake and/or increased efflux of therapeutics, enhanced DNA repair, and altered activity or deregulation of target proteins. Although these mechanisms have been widely investigated, little is known about membrane barriers responsible for the chemical imperviousness of cell compartments and cellular segregation in cytostatic-treated tumors. In highly heterogeneous cross-resistant and radiorefractory cell populations selected by exposure to anticancer agents, we found a number of atypical recurrent cell types in (1) tumor cell cultures of different embryonic origins, (2) mouse xenografts, and (3) paraffin sections from patient tumors. Alongside morphologic peculiarities, these populations presented cancer stem cell markers, aberrant signaling pathways, and a set of deregulated miRNAs known to confer both stem-cell phenotypes and highly aggressive tumor behavior. The first type, named spiral cells, is marked by a spiral arrangement of nuclei. The second type, monastery cells, is characterized by prominent walls inside which daughter cells can be seen maturing amid a rich mitochondrial environment. The third type, called pregnant cells, is a giant cell with a syncytium-like morphology, a main nucleus, and many endoreplicative functional progeny cells. A rare fourth cell type identified in leukemia was christened shepherd cells, as it was always associated with clusters of smaller cells. Furthermore, a portion of resistant tumor cells displayed nuclear encapsulation via mitochondrial aggregation in the nuclear perimeter in response to cytostatic insults, probably conferring imperviousness to drugs and long periods of dormancy until nuclear eclosion takes place. This phenomenon was correlated with an increase in both intracellular and intercellular mitochondrial traffic as well as with the uptake of free extracellular mitochondria. All these cellular disorders could, in fact, be found in untreated tumor cells but were more pronounced in resistant entities, suggesting a natural mechanism of cell survival triggered by chemical injury, or a primitive strategy to ensure stemming, self-renewal, and differentiation under adverse conditions, a fact that may play a significant role in chemotherapy outcomes.
authors.affiliation: 1 Institute of Molecular Oncology and Experimental Therapeutics, Marienhospital Herne, Ruhr University of Bochum Medical School , Herne, Germany .
authors.forename: David
authors.initials: D
authors.lastname: Díaz-Carballo
authors.forename: Sebastian
authors.initials: S
authors.lastname: Gustmann
authors.forename: Holger
authors.initials: H
authors.lastname: Jastrow
authors.forename: Ali Haydar
authors.initials: AH
authors.lastname: Acikelli
authors.forename: Philip
authors.initials: P
authors.lastname: Dammann
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Klein
authors.forename: Ulrike
authors.initials: U
authors.lastname: Dembinski
authors.forename: Walter
authors.initials: W
authors.lastname: Bardenheuer
authors.forename: Sascha
authors.initials: S
authors.lastname: Malak
authors.forename: Marcos J
authors.initials: MJ
authors.lastname: Araúzo-Bravo
authors.forename: Beate
authors.initials: B
authors.lastname: Schultheis
authors.forename: Constanze
authors.initials: C
authors.lastname: Aldinger
authors.forename: Dirk
authors.initials: D
authors.lastname: Strumberg
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D054697:Cytostatic Agents / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D005047:Etoposide / Q000494:pharmacology; D005260:Female; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D009369:Neoplasms / Q000235:genetics / Q000473:pathology / Q000648:ultrastructure*; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure*; D014407:Tumor Cells, Cultured
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D054697:Cytostatic Agents; D035683:MicroRNAs; D005047:Etoposide