title: Production of α-amylase for the biosynthesis of gold nanoparticles using Streptomyces sp. MBRC-82.
abstract: Marine actinobacterial synthesis of gold nanoparticles has good potential to develop simple, cost-effective and eco-friendly methods for production of important biomaterials. In this context, gold nanoparticles have attracted considerable attention in recent years, owing to their various applications. In this paper, we report on the production of α-amylase for the extracellular synthesis of gold nanoparticles using Streptomyces sp. MBRC-82. Medium composition and culture conditions for α-amylase production were statistically optimized. Plackett-Burman design was employed to find out the optimal medium constituents and culture conditions to enhance α-amylase production. Box-Behnken design revealed that three independent variables namely soluble starch (5.8484 g), peptone (3.5191 g), and NaCl (0.3829) significantly influenced α-amylase production. The gold nanoparticles were characterized by ultraviolet-visible (UV-vis) spectrometer, X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDXA), and transmission electron microscopy (TEM). The particles synthesized using the optimized enzyme activity ranged from 20 to 80 nm with an average particle size of 40 nm and therefore can be extended to various medicinal applications.
authors.affiliation: Department of Marine-Bio. Convergence Science and Marine Bioprocess Research Center, Pukyong National University, Busan 608-739, Republic of Korea.
authors.forename: Panchanathan
authors.initials: P
authors.lastname: Manivasagan
authors.affiliation: Department of Marine-Bio. Convergence Science and Marine Bioprocess Research Center, Pukyong National University, Busan 608-739, Republic of Korea.
authors.forename: Jayachandran
authors.initials: J
authors.lastname: Venkatesan
authors.affiliation: Department of Marine-Bio. Convergence Science and Marine Bioprocess Research Center, Pukyong National University, Busan 608-739, Republic of Korea.
authors.forename: Kyong-Hwa
authors.initials: KH
authors.lastname: Kang
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608502, Tamil Nadu, India.
authors.forename: Kannan
authors.initials: K
authors.lastname: Sivakumar
authors.affiliation: Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Sun-Joo
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Marine-Bio. Convergence Science and Marine Bioprocess Research Center, Pukyong National University, Busan 608-739, Republic of Korea. Electronic address: sknkim@pknu.ac.kr.
authors.forename: Se-Kwon
authors.initials: SK
authors.lastname: Kim
mesh_terms: D000704:Analysis of Variance; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D010802:Phylogeny; D012044:Regression Analysis; D013052:Spectrometry, X-Ray Emission; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013302:Streptomyces / Q000201:enzymology* / Q000302:isolation & purification; D014961:X-Ray Diffraction; D000516:alpha-Amylases / Q000096:biosynthesis*
chemical_list: D006046:Gold; D000516:alpha-Amylases
keywords: Biosynthesis; Gold nanoparticles; Optimization; Streptomyces sp.; α-Amylase