title: Regulation of lipid metabolism in the green microalga Chlorella protothecoides by heterotrophy-photoinduction cultivation regime.
abstract: Proteomics in conjunction with biochemical strategy was employed to unravel regulation of lipid metabolism in the green microalga Chlorella protothecoides by heterotrophy-photoinduction cultivation regime (HPC). Interestingly, HPC triggered transiently synthesis of starch followed by substantial lipid accumulation. And a marked decrease in intracellular protein and chlorophyll contents was also observed after 12h of photo-induction. The highest lipid content of 50.5% was achieved upon the photo-induction stage, which represented 69.3% higher than that of the end of heterotrophic cultivation. Results suggested that turnover of carbon-nitrogen-rich compounds such as starch, protein, and chlorophyll might provide carbon or energy for lipid accumulation. The proteomics analysis indicated that several pathways including glycolysis, TCA cycle, β-oxidation of fatty acids, Calvin cycle, photosynthesis, energy and transport, protein biosynthesis, regulate and defense were involved in the lipid biosynthesis. Malate dehydrogenase and acyl-CoA dehydrogenase were suggested as key regulatory factors in enhancing lipid accumulation.
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan, China. Electronic address: yuqinli2004@xtu.edu.cn.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xu
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan, China.
authors.forename: Fangxin
authors.initials: F
authors.lastname: Han
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan, China.
authors.forename: Jinxiu
authors.initials: J
authors.lastname: Mu
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan, China.
authors.forename: Di
authors.initials: D
authors.lastname: Chen
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Feng
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zeng
mesh_terms: D020418:Algal Proteins / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods; D002708:Chlorella / Q000145:classification / Q000502:physiology* / Q000528:radiation effects; D025461:Feedback, Physiological / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology / Q000528:radiation effects; D052836:Heterotrophic Processes / Q000502:physiology* / Q000528:radiation effects; D008027:Light; D050356:Lipid Metabolism / Q000502:physiology*; D058433:Photobioreactors / Q000382:microbiology*; D010788:Photosynthesis / Q000502:physiology* / Q000528:radiation effects; D015398:Signal Transduction / Q000502:physiology / Q000528:radiation effects; D013045:Species Specificity
chemical_list: D020418:Algal Proteins
keywords: Chlorella protothecoides; Heterotrophy–photoinduction; Proteomics; Regulation of lipid metabolism