title: An antioxidative galactomannan-protein complex isolated from fermentation broth of a medicinal fungus Cs-HK1.
abstract: A protein-containing polysaccharide, EPS2BW, was fractionated from the exopolysaccharide (EPS) produced by a medicinal fungus Cordyceps sinensis (Cs-HK1). EPS2BW was mainly composed of galactomannan with about 16% (w/w) protein and 50 kDa average molecular weight. The galactomannan part consisted of mannose and galactose at 1.7:1.0 molar ratio, and the protein segments were composed of sixteen amino acids with 12.5% proline and 16.6% threonine (mol%) being the most abundant. Based on analytical results from NMR, methylation analysis, partial acid hydrolysis and GC-MS, the galactomannan structure was elucidated as a (1 → 2)-α-D-mannopyranosyl (Manp) backbone with O-6-linked galactopyranosyl (Galp) branches. EPS2BW exhibited a high antioxidant capacity in both chemical and cell culture assays, with a Trolox equivalent radical scavenging activity of 44.7 μmol Trolox/mg, a Fe(3+) reducing power of 38.9 μmol Fe(2+)/mg, and significant cytoprotective effect against H2O2-induced PC12 cell death at 50-250 μg/mL.
authors.affiliation: Department of Applied Biology & Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Applied Biology & Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China; School of Biotechnology, Jiangnan University, Wuxi, China.
authors.forename: Zhong-Yang
authors.initials: ZY
authors.lastname: Ding
authors.affiliation: Department of Applied Biology & Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
authors.forename: Wen-Qiang
authors.initials: WQ
authors.lastname: Wang
authors.affiliation: Department of Applied Biology & Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
authors.forename: Ka-Chai
authors.initials: KC
authors.lastname: Siu
authors.affiliation: Department of Applied Biology & Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China. Electronic address: jian-yong.wu@polyu.edu.hk.
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Wu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D032661:Cordyceps / Q000737:chemistry*; D005285:Fermentation; D005656:Fungal Proteins / Q000737:chemistry* / Q000494:pharmacology*; D005690:Galactose / Q000031:analogs & derivatives; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016716:PC12 Cells / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification; D051381:Rats; D013055:Spectrophotometry, Infrared
chemical_list: D000975:Antioxidants; D005656:Fungal Proteins; D008351:Mannans; D011134:Polysaccharides; C012990:galactomannan; D005690:Galactose
keywords: Antioxidation; Cordyceps sinensis; Cytoprotection; Exopolysaccharides; Galactomannan; Polysaccharide–protein complex