title: Taking control over intracellular fatty acid levels is essential for the analysis of thermogenic function in cultured primary brown and brite/beige adipocytes.
abstract: Thermogenesis in brown adipocytes, conferred by mitochondrial uncoupling protein 1 (UCP1), is receiving great attention because metabolically active brown adipose tissue may protect humans from metabolic diseases. In particular, the thermogenic function of brown-like adipocytes in white adipose tissue, known as brite (or beige) adipocytes, is currently of prime interest. A valid procedure to quantify the specific contribution of UCP1 to thermogenesis is thus of vital importance. Adrenergic stimulation of lipolysis is a common way to activate UCP1. We here report, however, that in this frequently applied setup, taking control over intracellular fatty acid levels is essential for the analysis of thermogenic function in cultured brown and brite adipocytes. By the application of these findings, we demonstrate that UCP1 is functionally thermogenic in intact brite adipocytes and adrenergic UCP1 activation is largely dependent on adipose triglyceride lipase (ATGL) rather than hormone sensitive lipase (HSL).
authors.affiliation: Molecular Nutritional Medicine, Else Kröner Fresenius Center for Nutritional Medicine, Technische Universität München, Freising, Germany.
authors.forename: Yongguo
authors.initials: Y
authors.lastname: Li
authors.affiliation: Molecular Nutritional Medicine, Else Kröner Fresenius Center for Nutritional Medicine, Technische Universität München, Freising, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Fromme
authors.affiliation: Molecular Nutritional Medicine, Else Kröner Fresenius Center for Nutritional Medicine, Technische Universität München, Freising, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Schweizer
authors.affiliation: Molecular Nutritional Medicine, Else Kröner Fresenius Center for Nutritional Medicine, Technische Universität München, Freising, Germany.
authors.forename: Theresa
authors.initials: T
authors.lastname: Schöttl
authors.affiliation: Molecular Nutritional Medicine, Else Kröner Fresenius Center for Nutritional Medicine, Technische Universität München, Freising, Germany mk@tum.de.
authors.forename: Martin
authors.initials: M
authors.lastname: Klingenspor
mesh_terms: D052437:Adipocytes, Brown / Q000378:metabolism*; D002001:Adipose Tissue, Brown / Q000378:metabolism*; D052436:Adipose Tissue, White / Q000378:metabolism*; D002460:Cell Line; D005227:Fatty Acids / Q000378:metabolism; D006801:Humans; D007473:Ion Channels / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism; D008066:Lipolysis / Q000235:genetics; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism*; D002787:Sterol Esterase / Q000378:metabolism; D022722:Thermogenesis / Q000235:genetics*; D000071256:Uncoupling Protein 1; D000217:Acyltransferases
chemical_list: D005227:Fatty Acids; D007473:Ion Channels; D008049:Lipase; D024101:Mitochondrial Proteins; D002787:Sterol Esterase; D000071256:Uncoupling Protein 1; C000604371:UCP1 protein, human; C494794:PNPLA2 protein, human; D000217:Acyltransferases
keywords: UCP1; brite adipocytes; brown adipocytes; thermogenesis; uncoupled respiration