title: Effects of dimethylaminoethanol and compound amino acid on D-galactose induced skin aging model of rat.
abstract: A lasting dream of human beings is to reverse or postpone aging. In this study, dimethylaminoethanol (DMAE) and compound amino acid (AA) in Mesotherapy were investigated for their potential antiaging effects on D-galactose induced aging skin. At 18 days after D-gal induction, each rat was treated with intradermal microinjection of saline, AA, 0.1% DMAE, 0.2% DMAE, 0.1% DMAE + AA, or 0.2% DMAE + AA, respectively. At 42 days after treatment, the skin wound was harvested and assayed. Measurement of epidermal and dermal thickness in 0.1% DMAE + AA and 0.2% DMAE + AA groups appeared significantly thicker than aging control rats. No differences were found in tissue water content among groups. Hydroxyproline in 0.1% DMAE + AA, 0.2% DMAE + AA, and sham control groups was much higher than all other groups. Collagen type I, type III, and MMP-1 expression was highly upregulated in both 0.1% DMAE + AA and 0.2% DMAE + AA groups compared with aging control. In contrast, TIMP-1 expression levels of various aging groups were significantly reduced when compared to sham control. Coinjection of DMAE and AA into target tissue has marked antiaging effects on D-galactose induced skin aging model of rat.
authors.affiliation: Department of Plastic Surgery, Zhujiang Hospital, Southern Medical University, 253 GongYe Middle Avenue, Guangzhou, Guangdong 510280, China ; Department of Plastic and Aesthetic Surgery, The Affiliated Hospital of Medical School, Qingdao University, Qingdao, Shandong 266003, China.
authors.forename: Su
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Plastic and Aesthetic Surgery, The Affiliated Hospital of Medical School, Qingdao University, Qingdao, Shandong 266003, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Plastic and Aesthetic Surgery, The Affiliated Hospital of Medical School, Qingdao University, Qingdao, Shandong 266003, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Cai
authors.affiliation: Department of Dermatology, Huang-Si Aesthetic Surgery Hospital, Beijing 100120, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Plastic and Aesthetic Surgery, The Affiliated Hospital of Medical School, Qingdao University, Qingdao, Shandong 266003, China.
authors.forename: Cailing
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Center for Advanced Orthopedic Studies, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA ; Institute of Plastic and Reconstructive Surgery, Weifang Medical University, Shandong 261042, China.
authors.forename: Fangjun
authors.identifier: 0000-0002-2769-5012
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Plastic Surgery, Zhujiang Hospital, Southern Medical University, 253 GongYe Middle Avenue, Guangzhou, Guangdong 510280, China.
authors.forename: Dalie
authors.identifier: 0000-0001-5121-4701
authors.initials: D
authors.lastname: Liu
mesh_terms: D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D003094:Collagen / Q000235:genetics / Q000378:metabolism; D003642:Deanol / Q000494:pharmacology*; D005690:Galactose / Q000494:pharmacology; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D015595:Skin Aging / Q000187:drug effects*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism
chemical_list: D000596:Amino Acids; D019715:Tissue Inhibitor of Metalloproteinase-1; D003642:Deanol; D003094:Collagen; D020781:Matrix Metalloproteinase 1; D005690:Galactose