title: Overlapped sequence types (STs) and serogroups of avian pathogenic (APEC) and human extra-intestinal pathogenic (ExPEC) Escherichia coli isolated in Brazil.
abstract: Avian pathogenic Escherichia coli (APEC) strains belong to a category that is associated with colibacillosis, a serious illness in the poultry industry worldwide. Additionally, some APEC groups have recently been described as potential zoonotic agents. In this work, we compared APEC strains with extraintestinal pathogenic E. coli (ExPEC) strains isolated from clinical cases of humans with extra-intestinal diseases such as urinary tract infections (UTI) and bacteremia. PCR results showed that genes usually found in the ColV plasmid (tsh, iucA, iss, and hlyF) were associated with APEC strains while fyuA, irp-2, fepC sitDchrom, fimH, crl, csgA, afa, iha, sat, hlyA, hra, cnf1, kpsMTII, clpVSakai and malX were associated with human ExPEC. Both categories shared nine serogroups (O2, O6, O7, O8, O11, O19, O25, O73 and O153) and seven sequence types (ST10, ST88, ST93, ST117, ST131, ST155, ST359, ST648 and ST1011). Interestingly, ST95, which is associated with the zoonotic potential of APEC and is spread in avian E. coli of North America and Europe, was not detected among 76 APEC strains. When the strains were clustered based on the presence of virulence genes, most ExPEC strains (71.7%) were contained in one cluster while most APEC strains (63.2%) segregated to another. In general, the strains showed distinct genetic and fingerprint patterns, but avian and human strains of ST359, or ST23 clonal complex (CC), presented more than 70% of similarity by PFGE. The results demonstrate that some "zoonotic-related" STs (ST117, ST131, ST10CC, ST23CC) are present in Brazil. Also, the presence of moderate fingerprint similarities between ST359 E. coli of avian and human origin indicates that strains of this ST are candidates for having zoonotic potential.
authors.affiliation: Bacterial Molecular Biology Laboratory, Department of Genetics, Evolution and Bioagents, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Renato Pariz
authors.initials: RP
authors.lastname: Maluta
authors.affiliation: Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, United States of America.
authors.forename: Catherine Mary
authors.initials: CM
authors.lastname: Logue
authors.affiliation: Instituto Adolfo Lutz, São Paulo, SP, Brazil.
authors.forename: Monique Ribeiro Tiba
authors.initials: MR
authors.lastname: Casas
authors.affiliation: Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, United States of America.
authors.forename: Ting
authors.initials: T
authors.lastname: Meng
authors.affiliation: Instituto Biológico, CAPTAA, Unidade de Pesquisa e Desenvolvimento de Bastos, Bastos, SP, Brazil.
authors.forename: Elisabete Aparecida Lopes
authors.initials: EA
authors.lastname: Guastalli
authors.affiliation: Bacterial Molecular Biology Laboratory, Department of Genetics, Evolution and Bioagents, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Thaís Cabrera Galvão
authors.initials: TC
authors.lastname: Rojas
authors.affiliation: Departamento de Clínica Médica, Faculdade de Medicina, Universidade Estadual Paulista, Botucatu, SP, Brazil.
authors.forename: Augusto Cezar
authors.initials: AC
authors.lastname: Montelli
authors.affiliation: Departamento de Microbiologia e Imunologia, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, SP, Brazil.
authors.forename: Teruê
authors.initials: T
authors.lastname: Sadatsune
authors.affiliation: Department of Internal Medicine, UNICAMP, Campinas, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: de Carvalho Ramos
authors.affiliation: Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, United States of America.
authors.forename: Lisa Kay
authors.initials: LK
authors.lastname: Nolan
authors.affiliation: Bacterial Molecular Biology Laboratory, Department of Genetics, Evolution and Bioagents, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Wanderley Dias
authors.initials: WD
authors.lastname: da Silveira
mesh_terms: D000818:Animals; D016470:Bacteremia / Q000382:microbiology; D001938:Brazil; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary; D005798:Genes, Bacterial; D005807:Genes, Overlapping; D058507:Host Specificity; D006801:Humans; D010802:Phylogeny; D011200:Poultry / Q000821:virology; D011201:Poultry Diseases / Q000382:microbiology; D065288:Serogroup; D014552:Urinary Tract Infections / Q000382:microbiology; D014774:Virulence / Q000235:genetics; D015047:Zoonoses / Q000382:microbiology