title: Simultaneous determination of phenolic compounds in Equisetum palustre L. by ultra high performance liquid chromatography with tandem mass spectrometry combined with matrix solid-phase dispersion extraction.
abstract: A method based on matrix solid-phase dispersion extraction followed by ultra high performance liquid chromatography with tandem mass spectrometry is presented for the extraction and determination of phenolic compounds in Equisetum palustre. This method combines the high efficiency of matrix solid-phase dispersion extraction and the rapidity, sensitivity, and accuracy of ultra high performance liquid chromatography with tandem mass spectrometry. The influential parameters of the matrix solid-phase dispersion extraction were investigated and optimized. The optimized conditions were as follows: silica gel was selected as dispersing sorbent, the ratio of silica gel to sample was selected to be 2:1 (400/200 mg), and 8 mL of 80% methanol was used as elution solvent. Furthermore, a fast and sensitive ultra high performance liquid chromatography with tandem mass spectrometry method was developed for the determination of nine phenolic compounds in E. palustre. This method was carried out within <6 min, and exhibited satisfactory linearity, precision, and recovery. Compared with ultrasound-assisted extraction, the proposed matrix solid-phase dispersion procedure possessed higher extraction efficiency, and was more convenient and time saving with reduced requirements on sample and solvent amounts. All these results suggest that the developed method represents an excellent alternative for the extraction and determination of active components in plant matrices.
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, P.R.China; Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin, P.R. China.
authors.forename: Zuofu
authors.initials: Z
authors.lastname: Wei
authors.forename: Youzhi
authors.initials: Y
authors.lastname: Pan
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Huang
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Qi
authors.forename: Meng
authors.initials: M
authors.lastname: Luo
authors.forename: Yuangang
authors.initials: Y
authors.lastname: Zu
authors.forename: Yujie
authors.initials: Y
authors.lastname: Fu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020829:Equisetum / Q000737:chemistry*; D057230:Limit of Detection; D010636:Phenols / Q000737:chemistry* / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010636:Phenols; D010936:Plant Extracts
keywords: Equisetum palustre L; Mass spectrometry; Matrix solid-phase dispersion; Phenolic compounds; Ultra high performance liquid chromatography