title: The chemokine CCL18 characterises Pseudomonas infections in cystic fibrosis lung disease.
abstract: Cystic fibrosis (CF) lung disease is characterised by chronic Pseudomonas aeruginosa infection and leukocyte infiltration. Chemokines recruit leukocytes to sites of infection. Gene expression analysis identified the chemokine CCL18 as upregulated in CF leukocytes. We hypothesised that CCL18 characterises infection and inflammation in patients with CF lung disease. Therefore, we quantified CCL18 protein levels in the serum and airway fluids of CF patients and healthy controls, and studied CCL18 protein production by airway cells ex vivo. These studies demonstrated that CCL18 levels were increased in the serum and airway fluids from CF patients compared with healthy controls. Within CF patients, CCL18 levels were increased in P. aeruginosa-infected CF patients. CCL18 levels in the airways, but not in serum, correlated with severity of pulmonary obstruction in CF. Airway cells isolated from P. aeruginosa-infected CF patients produced significantly higher amounts of CCL18 protein compared with airway cells from CF patients without P. aeruginosa infection or healthy controls. Collectively, these studies show that CCL18 levels characterise chronic P. aeruginosa infection and pulmonary obstruction in patients with CF. CCL18 may, thus, serve as a potential biomarker and therapeutic target in CF lung disease.
authors.affiliation: Dept of Pediatrics I and Interdisciplinary Center for Infectious Diseases, University of Tübingen, Tübingen, Germany Both authors contributed equally.
authors.forename: Andreas
authors.initials: A
authors.lastname: Hector
authors.affiliation: Dept of Pediatrics, Ludwig-Maximilians-University, Munich, Germany Both authors contributed equally.
authors.forename: Carolin
authors.initials: C
authors.lastname: Kröner
authors.affiliation: Dept of Pediatrics I and Interdisciplinary Center for Infectious Diseases, University of Tübingen, Tübingen, Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Carevic
authors.affiliation: Dept of Pediatrics I and Interdisciplinary Center for Infectious Diseases, University of Tübingen, Tübingen, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Bakele
authors.affiliation: Dept of Pediatrics I and Interdisciplinary Center for Infectious Diseases, University of Tübingen, Tübingen, Germany.
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Rieber
authors.affiliation: Dept of Pediatrics I and Interdisciplinary Center for Infectious Diseases, University of Tübingen, Tübingen, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Riethmüller
authors.affiliation: Dept of Pediatrics, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Griese
authors.affiliation: Dept of Pneumology, Center for Medicine, Medical Center - University of Freiburg, Freiburg, Germany.
authors.forename: Gernot
authors.initials: G
authors.lastname: Zissel
authors.affiliation: Dept of Pediatrics I and Interdisciplinary Center for Infectious Diseases, University of Tübingen, Tübingen, Germany dominik.hartl@med.uni-tuebingen.de.
authors.forename: Dominik
authors.initials: D
authors.lastname: Hartl
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D054360:Chemokine CXCL1 / Q000276:immunology / Q000378:metabolism; D054426:Chemokine CXCL2 / Q000276:immunology / Q000378:metabolism; D019742:Chemokines, CC / Q000276:immunology / Q000378:metabolism*; D002648:Child; D003550:Cystic Fibrosis / Q000150:complications / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000276:immunology / Q000378:metabolism; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism*; D008297:Male; D011552:Pseudomonas Infections / Q000150:complications / Q000276:immunology / Q000378:metabolism*; D013183:Sputum / Q000378:metabolism; D055815:Young Adult
chemical_list: C111792:CCL18 protein, human; C475829:CXCL1 protein, human; C499706:CXCL2 protein, human; C508606:CXCL8 protein, human; D054360:Chemokine CXCL1; D054426:Chemokine CXCL2; D019742:Chemokines, CC; D016209:Interleukin-8