title: Development of new method for simultaneous analysis of piracetam and levetiracetam in pharmaceuticals and biological fluids: application in stability studies.
abstract: RP-HPLC ultraviolet detection simultaneous quantification of piracetam and levetiracetam has been developed and validated. The chromatography was obtained on a Nucleosil C18 column of 25 cm×0.46 cm, 10 μm, dimension. The mobile phase was a (70:30 v/v) mixture of 0.1 g/L of triethylamine and acetonitrile. Smooth flow of mobile phase at 1 mL/min was set and 205 nm wavelength was selected. Results were evaluated through statistical parameters which qualify the method reproducibility and selectivity for the quantification of piracetam, levetiracetam, and their impurities hence proving stability-indicating properties. The proposed method is significantly important, permitting the separation of the main constituent piracetam from levetiracetam. Linear behavior was observed between 20 ng/mL and 10,000 ng/mL for both drugs. The proposed method was checked in bulk drugs, dosage formulations, physiological condition, and clinical investigations and excellent outcome was witnessed.
authors.affiliation: Faculty of Pharmacy, Federal Urdu University Arts, Science and Technology, Karachi 75300, Pakistan ; Faculty of Pharmacy, Ziauddin University, Karachi 75600, Pakistan.
authors.forename: Farhan Ahmed
authors.initials: FA
authors.lastname: Siddiqui
authors.affiliation: Department of Chemistry, University of Karachi, Karachi 75270, Pakistan.
authors.forename: Nawab
authors.initials: N
authors.lastname: Sher
authors.affiliation: Faculty of Pharmacy, Federal Urdu University Arts, Science and Technology, Karachi 75300, Pakistan ; Department of Environmental Sciences, Federal Urdu University Arts, Science and Technology, Karachi 75300, Pakistan.
authors.forename: Nighat
authors.initials: N
authors.lastname: Shafi
authors.affiliation: Faculty of Medicine, Ziauddin University, Karachi, Pakistan.
authors.forename: Alisha
authors.initials: A
authors.lastname: Wafa Sial
authors.affiliation: Department of Pharmacognosy, Research Institute of Pharmaceutical Sciences, Faculty of Pharmacy, University of Karachi, Karachi 75270, Pakistan.
authors.forename: Mansoor
authors.initials: M
authors.lastname: Ahmad
authors.affiliation: Faculty of Pharmacy, Federal Urdu University Arts, Science and Technology, Karachi 75300, Pakistan.
authors.lastname: Mehjebeen
authors.affiliation: Faculty of Pharmacy, Ziauddin University, Karachi 75600, Pakistan.
authors.forename: Huma
authors.identifier: 0000-0001-9571-2673
authors.initials: H
authors.lastname: Naseem
mesh_terms: D000293:Adolescent; D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods; D004340:Drug Contamination; D004355:Drug Stability; D006801:Humans; D000077287:Levetiracetam; D016014:Linear Models; D008297:Male; D010889:Piracetam / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry* / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity; D013607:Tablets / Q000737:chemistry; D055815:Young Adult
chemical_list: D013607:Tablets; D000077287:Levetiracetam; D010889:Piracetam