title: Bmi1 expression in long-term germ stem cells.
abstract: Asingle cells in undifferentiated spermatogonia are considered to be the most primitive forms of germ stem cells (GSCs). Although GFRα1 is thought to be a marker of Asingle cells, we found that Bmi1(High) is more specific than GFRα1 for Asingle cells. Bmi1(High) expression in Asingle cells is correlated with seminiferous stages, and its expression was followed by the proliferative stage of Asingle GSCs. In contrast, GFRα1 expression was seminiferous stage-independent. Fate analyses of EdU-positive Bmi1(High)-positive cell-derived Asingle cells revealed that these cells self-renewed or generated transient amplifying Apaired cells. Bmi1(High)-positive cells were resistant to irradiation-induced injury, after which they regenerated. Elimination of Bmi1(High)-positive cells from seminiferous tubules resulted in the appearance of tubules with seminiferous stage mismatches. Thus, in this study, we found that Bmi1(High) is a seminiferous stage-dependent marker for long-term GSCs and that Bmi1(High)-positive cells play important roles in maintaining GSCs and in regenerating spermatogenic progenitors after injury.
authors.affiliation: 1] Department of Stem Cell Pathology, Kansai Medical University, 2-5-1 Shin-machi, Osaka 573-1010, Japan [2] Department of Urology and Andrology, Kansai Medical University, 2-5-1 Shin-machi, Osaka 573-1010, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Komai
authors.affiliation: 1] Department of Stem Cell Pathology, Kansai Medical University, 2-5-1 Shin-machi, Osaka 573-1010, Japan [2] Third Department of Internal Medicine, Kansai Medical University, 2-5-1 Shin-machi, Osaka 573-1010, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Department of Stem Cell Pathology, Kansai Medical University, 2-5-1 Shin-machi, Osaka 573-1010, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Tokuyama
authors.affiliation: 1] Department of Stem Cell Pathology, Kansai Medical University, 2-5-1 Shin-machi, Osaka 573-1010, Japan [2] Department of Surgery, Kansai Medical University, 2-5-1 Shin-machi, Osaka 573-1010, Japan.
authors.forename: Hirotsugu
authors.initials: H
authors.lastname: Yanai
authors.affiliation: 1] Department of Stem Cell Pathology, Kansai Medical University, 2-5-1 Shin-machi, Osaka 573-1010, Japan [2] Department of Dermatology, Kansai Medical University, 2-5-1 Shin-machi, Osaka 573-1010, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Ohe
authors.affiliation: 1] Department of Stem Cell Pathology, Kansai Medical University, 2-5-1 Shin-machi, Osaka 573-1010, Japan [2] Department of Pediatrics, Kansai Medical University, 2-5-1 Shin-machi, Osaka 573-1010, Japan.
authors.forename: Taichi
authors.initials: T
authors.lastname: Omachi
authors.affiliation: Department of Stem Cell Pathology, Kansai Medical University, 2-5-1 Shin-machi, Osaka 573-1010, Japan.
authors.forename: Naho
authors.initials: N
authors.lastname: Atsumi
authors.affiliation: Department of Stem Cell Pathology, Kansai Medical University, 2-5-1 Shin-machi, Osaka 573-1010, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Yoshida
authors.affiliation: Department of Stem Cell Pathology, Kansai Medical University, 2-5-1 Shin-machi, Osaka 573-1010, Japan.
authors.forename: Keiki
authors.initials: K
authors.lastname: Kumano
authors.affiliation: Department of Stem Cell Pathology, Kansai Medical University, 2-5-1 Shin-machi, Osaka 573-1010, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Hisha
authors.affiliation: Department of Urology and Andrology, Kansai Medical University, 2-5-1 Shin-machi, Osaka 573-1010, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Matsuda
authors.affiliation: Department of Stem Cell Pathology, Kansai Medical University, 2-5-1 Shin-machi, Osaka 573-1010, Japan.
authors.forename: Hiroo
authors.initials: H
authors.lastname: Ueno
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D017353:Gene Deletion; D015870:Gene Expression; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors / Q000378:metabolism; D016130:Immunophenotyping; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D063150:Polycomb Repressive Complex 1 / Q000235:genetics* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D012038:Regeneration; D012671:Seminiferous Tubules / Q000166:cytology; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects
chemical_list: C489421:Bmi1 protein, mouse; C494988:Gfra1 protein, mouse; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors; D011518:Proto-Oncogene Proteins; D063150:Polycomb Repressive Complex 1