title: Circulating miR-222 in plasma and its potential diagnostic and prognostic value in gastric cancer.
abstract: Previous studies have revealed the significance of circulating microRNAs as biomarkers for cancers. The aim of this study was to detect the levels of circulating microRNA-222 (miR-222) in plasma of gastric cancer (GC) patients and evaluate its diagnostic and prognostic value. Levels of circulating miR-222 were detected by using qRT-PCR in plasma of 114 GC patients, 36 chronic atrophic gastritis (CAG) patients and 56 healthy controls. The result showed that the expression of circulating miR-222 in plasma was significantly upregulated in GC compared with CAG and healthy controls (all at P < 0.001). And its high level was significantly correlated with clinical stages (P < 0.001) and lymph nodes metastasis (P = 0.009). The receiver operating characteristics (ROC) curve analyses revealed that miR-222 had considerable diagnostic accuracy, yielded an AUC (the areas under the ROC curve) of 0.850 with 66.1 % sensitivity and 88.3 % specificity in discriminating GC from healthy controls. Moreover, Kaplan-Meier analysis demonstrated a correlation between increased circulating miR-222 level and reduced disease-free survival (P = 0.016) and overall survival (P = 0.012). In multivariate analysis stratified for known prognostic variables, circulating miR-222 was identified as an independent prognostic marker. In conclusion, our findings suggested that circulating miR-222 in plasma might be a potential and useful noninvasive biomarker for the early detection and prognosis of GC.
authors.affiliation: Department of Oncology, Zaozhuang Mining Group Central Hospital, Qilianshan Road, Zaozhuang, 277000, Shandong, China, fuzhengchuan2015@163.com.
authors.forename: Zhengchuan
authors.initials: Z
authors.lastname: Fu
authors.forename: Fang
authors.initials: F
authors.lastname: Qian
authors.forename: Xuhuan
authors.initials: X
authors.lastname: Yang
authors.forename: Hailiang
authors.initials: H
authors.lastname: Jiang
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Sihai
authors.initials: S
authors.lastname: Liu
mesh_terms: D000704:Analysis of Variance; D016022:Case-Control Studies; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D011379:Prognosis; D012372:ROC Curve; D013274:Stomach Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000453:epidemiology / Q000401:mortality*
chemical_list: D035683:MicroRNAs