title: Mosquito cell lines: history, isolation, availability and application to assess the threat of arboviral transmission in the United Kingdom.
abstract: Mosquitoes are highly effective vectors for transmission of human and animal pathogens. Understanding the relationship between pathogen and vector is vital in developing strategies to predict and prevent transmission. Cell lines derived from appropriate mosquito hosts provide a relatively simple tool for investigating the interaction between the host and viruses transmitted by mosquitoes. This review provides a brief overview of the development of mosquito cell lines, methods of isolation, their availability and application for investigating insect-virus interactions.
authors.forename: Thomas
authors.initials: T
authors.lastname: Walker
authors.affiliation: Animal Health and Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK. Claire.Jeffries@lshtm.ac.uk.
authors.forename: Claire L
authors.initials: CL
authors.lastname: Jeffries
authors.forename: Karen L
authors.initials: KL
authors.lastname: Mansfield
authors.forename: Nicholas
authors.initials: N
authors.lastname: Johnson
mesh_terms: D000818:Animals; D001102:Arbovirus Infections / Q000635:transmission* / Q000821:virology; D001103:Arboviruses / Q000502:physiology*; D002460:Cell Line; D009033:Culicidae / Q000166:cytology*; D006801:Humans; D006113:United Kingdom; D014776:Virus Cultivation / Q000379:methods*