title: How substrate specificity is imposed on a histone demethylase--lessons from KDM2A.
abstract: Histone lysine methylation and demethylation regulate histone methylation dynamics, which impacts chromatin structure and function. To read and erase the methylated histone residues, lysine demethylases must specifically recognize the histone sequences and methylated sites and discriminate the degree of these methylations. In this issue of Genes & Development, Cheng and colleagues (pp. 1758-1771) determine a crystal structure of histone lysine demethylase KDM2A that specifically targets lower degrees of H3K36 methylation. The results reveal the structural basis for H3K36 substrate specificity and suggest mechanisms of Lys36 demethylation. This KDM2A-H3K36 complex structure, coupled with functional studies, provides needed insight into the process and regulation of histone demethylation.
authors.affiliation: Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA;
authors.forename: Chi-Lin
authors.initials: CL
authors.lastname: Tsai
authors.affiliation: Division of Newborn Medicine, Boston Children's Hospital, Cell Biology Department, Harvard Medical School, Boston, Massachusetts 02115, USA; jat@scripps.edu yang_shi@hms.harvard.edu.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA; Department of Integrative Structural and Computational Biology, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
authors.forename: John A
authors.initials: JA
authors.lastname: Tainer
mesh_terms: D000818:Animals; D006657:Histones / Q000378:metabolism*; D056484:Jumonji Domain-Containing Histone Demethylases / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular
chemical_list: D006657:Histones; C547579:FBXL11 protein, mouse; D056484:Jumonji Domain-Containing Histone Demethylases
keywords: KDM2A; histone demethylase; mechanism; substrate specificity