title: A cell wall protein (YqgA) is genetically related to the cell wall-degrading dl-endopeptidases in Bacillus subtilis.
abstract: The Gram-positive bacterium Bacillus subtilis has a thick cell wall. The cell wall contains various proteins, both for secretion and for peptidoglycan (PG) maintenance. Penicillin-binding proteins for PG synthesis, PG hydrolases (autolysins), and regulator proteins for the autolysins are the known components of the PG maintenance system. YqgA was identified as an abundant protein attached to the cell wall of B. subtilis through a proteomics analysis. The YqgA protein was localized at cell division sites during the transition period between the exponential and the stationary phases. YqgA localization was affected by mutations in the dl-endopeptidases (DLEPases), which are the autolysins involved in cell morphogenesis. Furthermore, yqgA mutations on a background of defective DLEPases led to delays in cell growth and cell morphological changes. These results demonstrate that yqgA is genetically related to the genes encoding DLEPases involved in cell morphogenesis.
authors.affiliation: a Institute of Molecular Medicine , National Cheng Kung University Medical College , Tainan , Taiwan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Kentaro
authors.initials: K
authors.lastname: Fujikura
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Miyake
authors.forename: Yuhei
authors.initials: Y
authors.lastname: Higashitsuji
authors.forename: Yuuka
authors.initials: Y
authors.lastname: Kiriyama
authors.forename: Tatsuhito
authors.initials: T
authors.lastname: Tanaka
authors.forename: Hiroki
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Junichi
authors.initials: J
authors.lastname: Sekiguchi
mesh_terms: D001412:Bacillus subtilis / Q000166:cytology* / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D049109:Cell Proliferation; D002473:Cell Wall / Q000378:metabolism*; D010450:Endopeptidases / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D042541:Intracellular Space / Q000378:metabolism; D009154:Mutation; D021381:Protein Transport; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D010450:Endopeptidases
keywords: Bacillus subtilis; autolysin; cell division; morphology