title: Construction and characterization of a repetitive DNA library in Parodontidae (Atinopterygii:Characiformes): a genomic and evolutionary approach to the degeneration of the w sex cromosome.
abstract: Repetitive DNA sequences, including tandem and dispersed repeats, comprise a large portion of eukaryotic genomes and are important for gene regulation, sex chromosome differentiation, and karyotype evolution. In Parodontidae, only the repetitive DNAs WAp and pPh2004 and rDNAs were previously studied using fluorescence in situ hybridization. This study aimed to build a library of repetitive DNA in Parodontidae. We isolated 40 clones using Cot-1; 17 of these clones exhibited similarity to repetitive DNA sequences, including satellites, minisatellites, microsatellites, and class I and class II transposable elements (TEs), from Danio rerio and other organisms. The physical mapping of the clones to chromosomes revealed the presence of a satellite DNA, a Helitron element, and degenerate short interspersed element (SINE), long interspersed element (LINE), and tc1-mariner elements on the sex chromosomes. Some clones exhibited dispersed signals; other sequences were not detected. The 5S rDNA was detected on an autosomal pair. These elements likely function in the molecular degeneration of the W chromosome in Parodontidae. Thus, the location of these elements on the chromosomes is important for understanding the function of these repetitive DNAs and for integrative studies with genome sequencing. The presented data demonstrate that an intensive invasion of TEs occurred during W sex chromosome differentiation in the Parodontidae.
authors.affiliation: 1 Programa de Pós-Graduação em Genética, Departamento de Genética, Centro Politécnico, Universidade Federal do Paraná , Curitiba, Paraná State, Brazil .
authors.forename: Michelle Orane
authors.initials: MO
authors.lastname: Schemberger
authors.forename: Jordana Inácio Nascimento
authors.initials: JI
authors.lastname: Oliveira
authors.forename: Viviane
authors.initials: V
authors.lastname: Nogaroto
authors.forename: Mara Cristina
authors.initials: MC
authors.lastname: Almeida
authors.forename: Roberto Ferreira
authors.initials: RF
authors.lastname: Artoni
authors.forename: Marta Margarete
authors.initials: MM
authors.lastname: Cestari
authors.forename: Orlando
authors.initials: O
authors.lastname: Moreira-Filho
authors.forename: Marcelo Ricardo
authors.initials: MR
authors.lastname: Vicari
mesh_terms: D000818:Animals; D001483:Base Sequence; D060125:Characiformes / Q000235:genetics*; D019143:Evolution, Molecular; D015723:Gene Library; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012730:Sex Chromosomes / Q000235:genetics*; D013045:Species Specificity
chemical_list: D012341:RNA, Ribosomal, 5S