title: Effects of inactivated Bordetella pertussis on phosphodiesterase in the lung of ovalbumin sensitized and challenged rats.
abstract: This paper indicated that inactivated Bordetella pertussis (iBp) can enhance the lung airway hyperreactivity of the rats sensitized and challenged with OVA. The mechanisms were involved in the upregulation of cAMP-PDE activity and PDE4A, PDE4D, and PDE3 gene expression in the lungs. But only PDE4 activity was different between the OVA and OVA+iBp groups, and PDE4D expression was significantly increased in iBp rats alone. So, our data suggested that cosensitization with OVA and iBp affects lung airway reactivity by modulating the lung cAMP-PDE activity and PDE4D gene expression.
authors.affiliation: Zhejiang Respiratory Drugs Research Laboratory of SFDA of China, School of Medicine, Zhejiang University, Hangzhou 310058, China ; Clinical College of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Hefei 230038, China.
authors.forename: Ya-Juan
authors.initials: YJ
authors.lastname: Wang
authors.affiliation: Zhejiang Respiratory Drugs Research Laboratory of SFDA of China, School of Medicine, Zhejiang University, Hangzhou 310058, China.
authors.forename: Shun-De
authors.initials: SD
authors.lastname: Song
authors.affiliation: First Affiliated Hospital, Zhejiang University, School of Medicine, Hangzhou 310000, China.
authors.forename: Jun-Chun
authors.initials: JC
authors.lastname: Chen
authors.affiliation: Second Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou 310005, China.
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Wang
authors.affiliation: Zhejiang Respiratory Drugs Research Laboratory of SFDA of China, School of Medicine, Zhejiang University, Hangzhou 310058, China.
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Jiang
authors.affiliation: Zhejiang Respiratory Drugs Research Laboratory of SFDA of China, School of Medicine, Zhejiang University, Hangzhou 310058, China.
authors.forename: Qiang-Min
authors.initials: QM
authors.lastname: Xie
authors.affiliation: Zhejiang Respiratory Drugs Research Laboratory of SFDA of China, School of Medicine, Zhejiang University, Hangzhou 310058, China.
authors.forename: Ji-Qiang
authors.initials: JQ
authors.lastname: Chen
authors.affiliation: Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
authors.forename: Zi-Gang
authors.initials: ZG
authors.lastname: Li
authors.affiliation: Zhejiang Respiratory Drugs Research Laboratory of SFDA of China, School of Medicine, Zhejiang University, Hangzhou 310058, China.
authors.forename: Hui-Fang
authors.identifier: 0000-0001-9541-1352
authors.initials: HF
authors.lastname: Tang
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D000818:Animals; D001886:Bordetella pertussis / Q000276:immunology; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3 / Q000378:metabolism; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4 / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D008168:Lung / Q000201:enzymology* / Q000276:immunology; D008297:Male; D010047:Ovalbumin / Q000276:immunology; D010567:Pertussis Vaccine / Q000276:immunology / Q000494:pharmacology*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000187:drug effects / Q000276:immunology; D015164:Vaccines, Inactivated
chemical_list: D010567:Pertussis Vaccine; D015164:Vaccines, Inactivated; D010047:Ovalbumin; D010727:Phosphoric Diester Hydrolases; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4; C528468:PDE4D protein, rat; C521385:Pde4a protein, rat