title: High-level conversion of L-lysine into 5-aminovalerate that can be used for nylon 6,5 synthesis.
abstract: L-Lysine is a potential feedstock for the production of bio-based precursors for engineering plastics. In this study, we developed a microbial process for high-level conversion of L-lysine into 5-aminovalerate (5AVA) that can be used as a monomer in nylon 6,5 synthesis. Recombinant Escherichia coli WL3110 strain expressing Pseudomonas putida delta-aminovaleramidase (DavA) and lysine 2-monooxygenase (DavB) was grown to high density in fed-batch culture and used as a whole cell catalyst. High-density E. coli WL3110 expressing DavAB, grown to an optical density at 600 nm (OD600 ) of 30, yielded 36.51 g/L 5AVA from 60 g/L L-lysine in 24 h. Doubling the cell density of E. coli WL3110 improved the conversion yield to 47.96 g/L 5AVA from 60 g/L of L-lysine in 24 h. 5AVA production was further improved by doubling the L-lysine concentration from 60 to 120 g/L. The highest 5AVA titer (90.59 g/L; molar yield 0.942) was obtained from 120 g/L L-lysine by E. coli WL3110 cells grown to OD600 of 60. Finally, nylon 6,5 was synthesized by bulk polymerization of ϵ-caprolactam and δ-valerolactam prepared from microbially synthesized 5AVA. The hybrid system demonstrated here has promising possibilities for application in the development of industrial bio-nylon production processes.
authors.affiliation: Department of Environmental Engineering and Energy, Myongji University, Cheoin-gu, Yongin, Gyeonggido, Republic of Korea.
authors.forename: Si Jae
authors.initials: SJ
authors.lastname: Park
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Oh
authors.forename: Won
authors.initials: W
authors.lastname: Noh
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Kim
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Shin
authors.forename: Eun Gyo
authors.initials: EG
authors.lastname: Lee
authors.forename: Seungwoon
authors.initials: S
authors.lastname: Lee
authors.forename: Yokimiko
authors.initials: Y
authors.lastname: David
authors.forename: Mary Grace
authors.initials: MG
authors.lastname: Baylon
authors.forename: Bong Keun
authors.initials: BK
authors.lastname: Song
authors.forename: Jonggeon
authors.initials: J
authors.lastname: Jegal
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000581:Amidohydrolases / Q000235:genetics / Q000378:metabolism; D021542:Amino Acids, Neutral / Q000032:analysis / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008239:Lysine / Q000032:analysis / Q000378:metabolism*; D009757:Nylons / Q000737:chemistry / Q000378:metabolism*; D058105:Polymerization; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D021542:Amino Acids, Neutral; D009757:Nylons; D011994:Recombinant Proteins; C013809:5-aminovaleric acid; D000581:Amidohydrolases; D008239:Lysine
keywords: 5-Aminovaleric acid; Bioconversion; L-Lysine; Nylon 6,5; Valerolactam