title: [Confirmation of 17 rare HLA alleles and prediction of their haplotypes].
abstract: OBJECTIVE
  To confirm 17 rare HLA alleles detected during routine HLA typing and deduce their haplotypes.
  
  
  METHODS
  Bi-allelic sequence-based typing and Luminex DNA PCR-SSOP assay were applied for the initial or repeat HLA typing, respectively. The rare HLA alleles were confirmed with mono-allelic sequence-based typing. Predicted haplotypes of the rare alleles were inferred based on the frequencies of HLA alleles and haplotypes in Han population.
  
  
  RESULTS
  The authenticity of the total 17 rare HLA alleles was proven, and 18 predicted haplotypes associated with the rare alleles were recognized. A*11:12 and DRB1*13:19 were detected twice among unrelated individuals.
  
  
  CONCLUSIONS
  Study of rare HLA alleles and predicted haplotype can provide useful information for donor searching and transplantation, and enrich polymorphisms of HLA in this population.
authors.affiliation: China Marrow Donor Program HLA Typing Laboratory; Blood Center of Shandong Province, Jinan, Shandong 250014, P.R. China. chuanfuzhu@aliyun.com.
authors.forename: Chuanfu
authors.initials: C
authors.lastname: Zhu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Nie
authors.forename: Wenben
authors.initials: W
authors.lastname: Qiao
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Song
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Zhuang
mesh_terms: D000483:Alleles; D044466:Asian People / Q000208:ethnology / Q000235:genetics*; D005787:Gene Frequency; D015234:HLA-A Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains / Q000235:genetics*; D006239:Haplotypes; D006801:Humans
chemical_list: D015234:HLA-A Antigens; D015235:HLA-B Antigens; D059811:HLA-DRB1 Chains