title: Phosphatase and tensin analog expression in arterial atherosclerotic lesions.
abstract: BACKGROUND
  Phosphatase and tensin analog (PTEN) gene mutation has been proven for pro-inflammatory property and proliferative potential through tyrosine kinase pathway. We studied mutated PTEN for its pathogenetic association in arterial atherosclerosis.
  
  
  OBJECTIVE
  The objective was to study mutation of PTEN by immunohistochemical method in arterial atherosclerotic lesions and correlate with grades of atherosclerosis, smooth muscle migration in intima, degree of inflammation and Framingham heart study risk factors.
  
  
  METHODS
  Human, Prospective Clinical study.
  
  
  METHODS
  We studied patients with arterial occlusive disease diagnosed by Doppler ultrasonography over a 2-year period. Immunohistochemistry was performed with mouse monoclonal antibodies for PTEN and smooth muscle actin (SMA).
  
  
  METHODS
  Chi-square test.
  
  
  CONCLUSIONS
  Aorta was the single most common vessel affected (21%). Mean age of patients studied was 48.6 years and 80% were male. Mutant PTEN was associated with higher grades of atherosclerotic lesions (P < 0.0001) graded by American Heart Association classification and with smooth muscle proliferation and migration in intima (P < 0.0001). No statistically significant association with the vessel wall inflammation and other risk factors of atherosclerosis.
authors.forename: Elancheran
authors.initials: E
authors.lastname: Muthalagan
authors.affiliation: Department of Pathology, JIPMER, Puducherry, India.
authors.forename: Rajesh Nachiappa
authors.initials: RN
authors.lastname: Ganesh
authors.forename: B V
authors.initials: BV
authors.lastname: Sai Chandran
authors.forename: Surendra Kumar
authors.initials: SK
authors.lastname: Verma
mesh_terms: D001011:Aorta / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000473:pathology; D008297:Male; D008840:Microfilament Proteins / Q000032:analysis*; D008853:Microscopy; D008875:Middle Aged; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis*; D058226:Plaque, Atherosclerotic / Q000473:pathology*; D011446:Prospective Studies; D012720:Severity of Illness Index; D000070876:Tensins; D017539:Tunica Intima / Q000473:pathology
chemical_list: D008840:Microfilament Proteins; D000070876:Tensins; D010744:Phosphoric Monoester Hydrolases