title: Investigations on a wheat bran biorefinery involving organosolv fractionation and enzymatic treatment.
abstract: The present study elucidates the organosolv treatment of wheat bran, the major by-product of the milling industry. The influence of temperature (160-200°C) and ethanol concentration (30-60% (w/w)) at a given process time of 30min was investigated. Enzymatic treatments of the organosolv extracts including solid residues led to an overall glucose yield of 75%. The conversion of hemicelluloses into xylose and arabinose was approximately 60% and 45%, respectively. Proteins could be almost completely dissolved, however, practically no free amino acids were obtained. Surprisingly, only around 30% of lignin and 65% of minerals were dissolved. Severe treatment conditions induced the disintegration of fat into glycerol and fatty acids as well as the formation of sugar degradation products. During the lignin precipitation step, proteins partially coprecipitated.
authors.affiliation: Department of Food Science and Technology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria; Christian Doppler Laboratory for Innovative Bran Biorefinery, Austria. Electronic address: michael.reisinger@boku.ac.at.
authors.forename: Michael
authors.initials: M
authors.lastname: Reisinger
authors.affiliation: Department of Food Science and Technology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria; Christian Doppler Laboratory for Innovative Bran Biorefinery, Austria.
authors.forename: Özge
authors.initials: Ö
authors.lastname: Tirpanalan
authors.affiliation: Department of Food Science and Technology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria; Christian Doppler Laboratory for Innovative Bran Biorefinery, Austria.
authors.forename: Florian
authors.initials: F
authors.lastname: Huber
authors.affiliation: Department of Food Science and Technology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria; Christian Doppler Laboratory for Innovative Bran Biorefinery, Austria.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kneifel
authors.affiliation: Department of Food Science and Technology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria; Christian Doppler Laboratory for Innovative Bran Biorefinery, Austria.
authors.forename: Senad
authors.initials: S
authors.lastname: Novalin
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D056804:Biofuels; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004043:Dietary Fiber / Q000032:analysis*; D000431:Ethanol; D005947:Glucose / Q000032:analysis; D006868:Hydrolysis; D008031:Lignin / Q000302:isolation & purification* / Q000378:metabolism; D010940:Plant Proteins / Q000302:isolation & purification* / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis / Q000378:metabolism; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D000596:Amino Acids; D056804:Biofuels; D004043:Dietary Fiber; D010940:Plant Proteins; D011134:Polysaccharides; D012997:Solvents; D000431:Ethanol; C007916:hemicellulose; D008031:Lignin; D005947:Glucose
keywords: Biorefinery; Enzymatic hydrolysis; Organosolv; Wheat bran