title: [Study on the B cell linear epitopes of rabies virus CVS-11 nucleoprotein].
abstract: To study the B cell linear epitopes of rabies virus CVS-11 nucleoprotein, peptides were synthesized according to the amino acid sequences of B cell linear epitopes. Linear epitopes predicted by bioinformatics analysis were evaluated with immunological techniques. Indirect enzyme-linked immunosorbent assay showed that titers of antibodies to peptides (355-369 and 385-400 residues of rabies virus CVS-11 nucleoprotein) were above 1:12 800 in mouse sera. The antibodies recognized denatured rabies virus CVS-11 nucleoprotein in Western blot analysis. Purified anti-peptide antibodies recognized natural rabies virus CVS-11 nucleoprotein in BHK-21 cells in indirect fluorescent antibody test. The 355-369 and 385-400 residues of rabies virus CVS-11 nucleoprotein were validated as B cell linear epitopes.
authors.forename: Xin-Jun
authors.initials: XJ
authors.lastname: Lv
authors.forename: Xin-Xin
authors.initials: XX
authors.lastname: Shen
authors.forename: Peng-Cheng
authors.initials: PC
authors.lastname: Yu
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Tang
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Liang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D011818:Rabies / Q000276:immunology / Q000821:virology*; D011820:Rabies virus / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D018985:Epitopes, B-Lymphocyte; D009698:Nucleoproteins