title: Lack of association between leptin levels and leptin gene polymorphism in obese women.
abstract: The aim of the present study was to evaluate the relationship between oligopolymorphism in the 25th codon of leptin gene and obesity. Eighty-seven obese women and 75 healthy women were constituted obese and control groups. Body fat percent, fat mass and lean body mass were determined by bioimpedance meter and leptin levels were determined. The presence of 25th codon oligopolymorphism in the leptin gene was done by PCR-RFLP technique. Mean leptin levels were 38.5±22.0 ng/ml, and 147.9±44.8 ng/ml in the control and obese groups, respectively. The correlations of serum leptin level to body fat percentage and fat mass in the control group were significant. The correlations in the obese group were not significant. This data implies that the difference of leptin levels between control and obese groups are more likely to be associated with alterations in the leptin gene other than 25th codon or alterations in the leptin receptor gene.
authors.affiliation: Department of Physiology, Faculty of Medicine, Selcuk University , Konya , Turkey .
authors.forename: Nilsel
authors.initials: N
authors.lastname: Okudan
authors.forename: Hakkı
authors.initials: H
authors.lastname: Gökbel
authors.forename: Hasan
authors.initials: H
authors.lastname: Acar
authors.forename: Selim
authors.initials: S
authors.lastname: Uzunoğlu
authors.forename: Muaz
authors.initials: M
authors.lastname: Belviranli
mesh_terms: D000273:Adipose Tissue; D000328:Adult; D001823:Body Composition; D015992:Body Mass Index; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D020738:Leptin / Q000097:blood* / Q000235:genetics*; D008875:Middle Aged; D009765:Obesity / Q000097:blood* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D020738:Leptin
keywords: Leptin gene; obesity; polymorphism