title: Bacterial and archaeal communities in Lake Nyos (Cameroon, Central Africa).
abstract: The aim of this study was to assess the microbial diversity associated with Lake Nyos, a lake with an unusual chemistry in Cameroon. Water samples were collected during the dry season on March 2013. Bacterial and archaeal communities were profiled using Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) approach of the 16S rRNA gene. The results indicate a stratification of both communities along the water column. Altogether, the physico-chemical data and microbial sequences suggest a close correspondence of the potential microbial functions to the physico-chemical pattern of the lake. We also obtained evidence of a rich microbial diversity likely to include several novel microorganisms of environmental importance in the large unexplored microbial reservoir of Lake Nyos.
authors.affiliation: Department of Environmental and Energy Sciences, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan.
authors.forename: Rosine E
authors.initials: RE
authors.lastname: Tiodjio
authors.affiliation: Department of Environmental and Energy Sciences, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Sakatoku
authors.affiliation: Department of Environmental and Energy Sciences, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Nakamura
authors.affiliation: Department of Environmental and Energy Sciences, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tanaka
authors.affiliation: Institute of Mining and Geological Research, P.O. Box 4110, Yaoundé, Cameroon.
authors.forename: Wilson Y
authors.initials: WY
authors.lastname: Fantong
authors.affiliation: Department of Environmental and Energy Sciences, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan.
authors.forename: Kamtchueng B
authors.initials: KB
authors.lastname: Tchakam
authors.affiliation: Institute of Mining and Geological Research, P.O. Box 4110, Yaoundé, Cameroon.
authors.forename: Gregory
authors.initials: G
authors.lastname: Tanyileke
authors.affiliation: Department of Chemistry, School of Science, University of Tokai, Kanagawa 259-1292, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ohba
authors.affiliation: Institute of Mining and Geological Research, P.O. Box 4110, Yaoundé, Cameroon.
authors.forename: Victor J
authors.initials: VJ
authors.lastname: Hell
authors.affiliation: Department of Environmental and Energy Sciences, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Kusakabe
authors.affiliation: Department of Environmental and Energy Sciences, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan.
authors.forename: Shogo
authors.initials: S
authors.lastname: Nakamura
authors.affiliation: Department of Environmental and Energy Sciences, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Ueda
mesh_terms: D001105:Archaea / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D001419:Bacteria / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D044822:Biodiversity; D002163:Cameroon; D005843:Geography; D060106:Lakes / Q000382:microbiology*; D014871:Water Microbiology