title: In vitro preliminary study of osteoblast response to surface roughness of titanium discs and topical application of melatonin.
abstract: OBJECTIVE
  To observe human osteoblast behavior cultured in vitro on titanium discs (Ti) in relation to surface roughness and melatonin application.
  
  
  METHODS
  Human osteoblasts (MG-63) were cultured on 60 Ti6Al4V discs divided into three groups: Group I: discs treated with dual acid etching; Group II dual acid etching and blasting with calcium phosphate particles; Group III (control) machined discs. Surface roughness and topography of the discs were examined with scanning electron microscope (SEM) and confocal laser scanning electron microscope( CLSM). Osteoblast adhesion, proliferation and cell morphology were determined by means of fluorescence microscopy with Image-Pro Plus software and SEM.
  
  
  RESULTS
  Group II presented the roughest discs, while the least rough were Group III. Cell adhesion was greatest in Group II. The addition of melatonin improved cell proliferation.
  
  
  CONCLUSIONS
  1. Surface treatments (dual acid etching, calcium phosphate impaction) increase surface roughness in comparison with machined titanium. 2. Greater surface roughness tends to favor cell adhesion after 24-hour cell culture. 3. The addition of melatonin tends to favor osteoblast proliferation.
authors.affiliation: Clínica Odontológica, C/ Gascó Oliag 1, 46010 Valencia, Spain, m.fernanda.sola@uv.es.
authors.forename: Maria-Fernanda
authors.initials: MF
authors.lastname: Solá-Ruiz
authors.forename: Carolina
authors.initials: C
authors.lastname: Pérez-Martínez
authors.forename: José-Javier
authors.initials: JJ
authors.lastname: Martín-del-Llano
authors.forename: Carmen
authors.initials: C
authors.lastname: Carda-Batalla
authors.forename: Carlos
authors.initials: C
authors.lastname: Labaig-Rueda
mesh_terms: D000287:Administration, Topical; D002478:Cells, Cultured; D015921:Dental Implants; D006801:Humans; D008550:Melatonin / Q000008:administration & dosage / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology*; D013499:Surface Properties; D014025:Titanium
chemical_list: D015921:Dental Implants; D014025:Titanium; D008550:Melatonin