title: Hepatic chemerin and chemokine-like receptor 1 expression in patients with chronic hepatitis C.
abstract: BACKGROUND
  Chemerin seems to be involved in pathogenesis of chronic hepatitis C (CHC). Hepatic expressions of chemerin and its receptor, chemokine receptor-like 1 (CMKLR1), in CHC have not been studied so far.
  
  
  OBJECTIVE
  To evaluate chemerin and CMKLR1 hepatic expression together with serum chemerin concentration in CHC patients and to assess their relationship with metabolic and histopathological abnormalities.
  
  
  METHODS
  The study included 63 nonobese CHC patients. Transcription of chemerin and CMKLR1 was assessed by quantitative real-time PCR, while serum chemerin was assessed by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Expression of chemerin and CMKLR1 was present in the liver of all CHC patients regardless of sex or age. This expression was not associated with necroinflammatory activity and steatosis grade, fibrosis stage, and metabolic abnormalities. There was a negative association between serum chemerin and chemerin hepatic expression (r = (-0.41), P = 0.006).
  
  
  CONCLUSIONS
  The study for the first time confirmed a marked expression of chemerin and CMKLR1 in the liver of CHC patients. The study was performed using the homogenates of human liver tissue, so it is not possible to define whether hepatocytes or other cell types which are abundantly represented in the liver constitute the main source of chemerin and CMKLR1 mRNA.
authors.affiliation: Department of Gastroenterology and Hepatology, Medical University of Silesia, Ulica Medyków 14, 40-752 Katowice, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Kukla
authors.affiliation: Department of General Biology, Medical University of Silesia, Katowice, Poland.
authors.forename: Brygida
authors.initials: B
authors.lastname: Adamek
authors.affiliation: Department of Gastroenterology and Hepatology, Medical University of Silesia, Ulica Medyków 14, 40-752 Katowice, Poland.
authors.forename: Marek
authors.identifier: 0000-0001-9969-7968
authors.initials: M
authors.lastname: Waluga
authors.affiliation: Department of General Biology, Medical University of Silesia, Katowice, Poland.
authors.forename: Marzena
authors.initials: M
authors.lastname: Zalewska-Ziob
authors.affiliation: Department of Environmental Medicine and Epidemiology, Medical University of Silesia, Katowice, Poland.
authors.forename: Janusz
authors.identifier: 0000-0002-0612-3031
authors.initials: J
authors.lastname: Kasperczyk
authors.affiliation: Department of Pathomorphology in Zabrze, Medical University of Silesia, Katowice, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gabriel
authors.affiliation: Department of Infectious Diseases in Chorzów, Medical University of Silesia, Katowice, Poland.
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Mazur
authors.affiliation: Department of Infectious Diseases in Bytom, Medical University of Silesia, Katowice, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Sobala-Szczygieł
authors.affiliation: Department of Physiology in Zabrze, Medical University of Silesia, Katowice, Poland.
authors.forename: Rafał J
authors.initials: RJ
authors.lastname: Bułdak
authors.affiliation: Department of Pathomorphology in Zabrze, Medical University of Silesia, Katowice, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Zajęcki
authors.affiliation: Department of Infectious Diseases in Bytom, Medical University of Silesia, Katowice, Poland.
authors.forename: Lucjan
authors.initials: L
authors.lastname: Kępa
authors.affiliation: Department of Paediatrics in Zabrze, Medical University of Silesia, Katowice, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Ziora
authors.affiliation: Department of Physiology in Zabrze, Medical University of Silesia, Katowice, Poland.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Żwirska-Korczala
authors.affiliation: Department of General Biology, Medical University of Silesia, Katowice, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Wiczkowski
authors.affiliation: Department of Gastroenterology and Hepatology, Medical University of Silesia, Ulica Medyków 14, 40-752 Katowice, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Hartleb
mesh_terms: D015992:Body Mass Index; D016022:Case-Control Studies; D018925:Chemokines / Q000097:blood / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D019698:Hepatitis C, Chronic / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism / Q000473:pathology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D019707:Receptors, Chemokine / Q000378:metabolism*
chemical_list: C102741:CMKLR1 protein, human; D018925:Chemokines; D036341:Intercellular Signaling Peptides and Proteins; C478630:RARRES2 protein, human; D019707:Receptors, Chemokine