title: Chronic toxicity and carcinogenicity of semicarbazide hydrochloride in Wistar Hannover GALAS rats.
abstract: We performed a combined study to determine the chronic toxicity and carcinogenicity of semicarbazide hydrochloride (SEM-HCl). Male and female Wistar Hannover GALAS rats were fed a diet containing SEM-HCl at 0, 10, 50, and 250ppm for 52weeks (10 rats/sex/group) or for 104weeks (50 rats/sex/group). Enlargement of the knee joints was apparent in both sexes at 250ppm. Reduced body weight was observed at 250ppm from week 76 only in males. SEM-HCl exerted no toxic effects on hematology, serum biochemistry, or organ weights. Histopathologically, disarrangement of chondrocytes accompanied by increased connective tissues, and degeneration of articular cartilage were found in males at 50ppm and above and in females at 250ppm. Mild changes in the elastic laminae were observed at 250ppm for both sexes in the chronic toxicity study. There were no significant intergroup differences in the incidences or types of any tumors. Taken together, toxicological effects of chronic exposure to SEM-HCI mainly occurred in the bone, cartilage, and aorta. Based on histopathological findings, the no-observed-adverse-effect-level was 10ppm in males and 50ppm in females (equal to 0.6mg/kg/day in males and 3.9mg/kg/day in females). SEM-HCl was not carcinogenic in rats.
authors.affiliation: Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan. Electronic address: mtakahashi@nihs.go.jp.
authors.forename: Miwa
authors.initials: M
authors.lastname: Takahashi
authors.affiliation: Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Midori
authors.initials: M
authors.lastname: Yoshida
authors.affiliation: Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Inoue
authors.affiliation: Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Morikawa
authors.affiliation: Biological Safety Research Center, National Institute of Health Sciences, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Nishikawa
authors.affiliation: Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Ogawa
mesh_terms: D000818:Animals; D015197:Carcinogenicity Tests; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D012663:Semicarbazides / Q000633:toxicity*; D018675:Toxicity Tests
chemical_list: D012663:Semicarbazides
keywords: Carcinogenicity; Chronic toxicity; Food contaminant; GALAS rats; Semicarbazide hydrochloride