title: Combination of LED light and platelet-derived growth factor to accelerate dentoalveolar osteogenesis.
abstract: OBJECTIVE
  This study aimed to evaluate the adjunctive effect of LED light in platelet-derived growth factor (PDGF)-aided dentoalveolar osteogenesis.
  
  
  METHODS
  Full-thickness osseous wounds were created on rat maxillae and were either unfilled or filled with poly-(D,L-lactide) and poly-(D,L-lactide-co-glycolide) microspheres encapsulating PDGF. Animals received daily 660 ± 25 nm LED light irradiation at 0, 10 (LD), or 20 (HD) J/cm(2) , were killed at days 4-28 (n = 6/group/time) and evaluated by microcomputed tomography (micro-CT), histology, and the expressions of osteopontin and tartrate-resistant acid phosphatase (TRAP).
  
  
  RESULTS
  Greater osteogenesis was noted in the PDGF-treated defects at day 14. Under the LED light irradiation, osteogenesis was significantly greater in both LD and HD groups of the non-PDGF-treated defects, but only in the LD group of the PDGF-treated defects. No significant differences in osteogenesis among groups were noted at day 28. Greater bone marrow space was noted in the LED light-irradiated specimens, especially in the PDGF-treated defects at both time points. Osteopontin was significantly promoted in the LD group at both time points, and TRAP was significantly promoted in all LED light-irradiated groups at day 28.
  
  
  CONCLUSIONS
  LED light could an adjunct to promote early PDGF-aided dentoalveolar osteogenesis by facilitating the osteoblast-osteoclast coupling.
authors.affiliation: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan.
authors.forename: Po-Chun
authors.initials: PC
authors.lastname: Chang
authors.forename: Chen-Ying
authors.initials: CY
authors.lastname: Wang
authors.forename: Tsai
authors.initials: T
authors.lastname: Sheng-Chueh
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D000077214:Becaplermin; D001672:Biocompatible Materials / Q000737:chemistry; D015519:Bone Density / Q000187:drug effects / Q000528:radiation effects; D001853:Bone Marrow / Q000473:pathology; D003131:Combined Modality Therapy; D004337:Drug Carriers; D007527:Isoenzymes / Q000032:analysis; D019344:Lactic Acid / Q000737:chemistry; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D008439:Maxillary Diseases / Q000188:drug therapy / Q000532:radiotherapy / Q000628:therapy*; D008863:Microspheres; D010006:Osteoblasts / Q000473:pathology; D010010:Osteoclasts / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D053495:Osteopontin / Q000032:analysis; D011091:Polyesters / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D020574:Proto-Oncogene Proteins c-sis / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000071681:Tartrate-Resistant Acid Phosphatase; D013997:Time Factors; D020390:Tooth Socket / Q000187:drug effects / Q000473:pathology* / Q000528:radiation effects; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D007527:Isoenzymes; D011091:Polyesters; D020574:Proto-Oncogene Proteins c-sis; C506897:Spp1 protein, rat; D053495:Osteopontin; D000077214:Becaplermin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase
keywords: alveolar bone; animal model; bone regeneration; immunohistochemistry; laser therapy, lower level; microcomputed tomography; platelet-derived growth factor