title: Chemokine Receptor 7 Implications of Spleen Dendritic Cells in Multiple-organ Dysfunction Syndrome in Mice.
abstract: OBJECTIVE
  This study aims to explore the chemokine receptor 7 (CCR7) expression of spleen dendritic cells (DCs) and their role in the changes of migration and activity of spleen DCs in multiple-organ dysfunction syndrome (MODS).
  
  
  METHODS
  The MODS model of mice was reproduced. The mice were randomly assigned to the following groups: normal, three-hour to six-hour, 24-hour to 48-hour, and 10-day to 12-day post-zymosan injection. CD11c and CD205 were analysed by immunohistochemistry; the expressions of CD86 and CCR7 of DCs were studied using flow cytometry analyses.
  
  
  RESULTS
  In normal mice, many DCs were found at the margin between the red and white pulp. In the three-hour to six-hour and 24-hour to 48-hour group, DC effectively upregulated CD86 and CCR7, and they were distributed in T- cell areas. In the 10-day to 12-day group, DCs were distributed at the margin by the immature form.
  
  
  CONCLUSIONS
  The CCR7 expression level of DCs had close correlations with the migration of DCs. Chemokine receptor 7 can be used to evaluate the migration and functional activity of DCs in MODS.
authors.affiliation: Department of Pathology, First Affiliated Hospital of General Hospital of PLA, Beijing 100037, China.
authors.forename: G
authors.initials: G
authors.lastname: Hou
authors.affiliation: Department of Pathology, First Affiliated Hospital of General Hospital of PLA, Beijing 100037, China. jiangyanglucn@yeah.net.
authors.forename: J
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Pathology, First Affiliated Hospital of General Hospital of PLA, Beijing 100037, China.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Pathology, First Affiliated Hospital of General Hospital of PLA, Beijing 100037, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Pathology, First Affiliated Hospital of General Hospital of PLA, Beijing 100037, China.
authors.forename: G
authors.initials: G
authors.lastname: Tian
authors.affiliation: Department of Pathology, First Affiliated Hospital of General Hospital of PLA, Beijing 100037, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yang