title: Telechelic poly(2-oxazoline)s with a biocidal and a polymerizable terminal as collagenase inhibiting additive for long-term active antimicrobial dental materials.
abstract: Dental repair materials face the problem that the dentin below the composite fillings is actively decomposed by secondary caries and extracellular proteases. To address this problem, poly(2-methyloxazoline) with a biocidal and a polymerizable terminal was explored as additive for a commercial dental adhesive. 2.5 wt% of the additive rendered the adhesive contact-active against Streptococcus mutans and washing with water for 101 d did not diminish this effect. The adhesive with 5 wt% additive kills S. mutans cells in the tubuli of bovine dentin. Further, the additive inhibits bacterial collagenase at 0.5 wt% and reduces activity of MMP-9. Human MMPs bound to dentin are inhibited by 96% in a medium with 5 wt% additive. Moreover, no adverse effect on the enamel/dentine shear bond strength was detected.
authors.affiliation: Chair of Biomaterials and Polymer Science, Department of Biochemical and Chemical Engineering, TU Dortmund, Emil-Figge-Str. 66, 44227, Dortmund, Germany.
authors.forename: Christoph P
authors.initials: CP
authors.lastname: Fik
authors.forename: Stefan
authors.initials: S
authors.lastname: Konieczny
authors.forename: David H
authors.initials: DH
authors.lastname: Pashley
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Waschinski
authors.forename: Reinhild S
authors.initials: RS
authors.lastname: Ladisch
authors.forename: Ulrich
authors.initials: U
authors.lastname: Salz
authors.forename: Thorsten
authors.initials: T
authors.lastname: Bock
authors.forename: Joerg C
authors.initials: JC
authors.lastname: Tiller
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002417:Cattle; D003094:Collagen / Q000378:metabolism; D017364:Collagenases / Q000378:metabolism*; D003764:Dental Materials / Q000494:pharmacology*; D004202:Disinfectants / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006909:Hydroxyproline / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors / Q000494:pharmacology*; D010080:Oxazoles / Q000494:pharmacology*; D010455:Peptides / Q000378:metabolism; D058105:Polymerization; D011994:Recombinant Proteins / Q000378:metabolism; D033081:Shear Strength / Q000187:drug effects; D017001:Tooth Demineralization
chemical_list: D000890:Anti-Infective Agents; D003764:Dental Materials; D004202:Disinfectants; D061965:Matrix Metalloproteinase Inhibitors; D010080:Oxazoles; D010455:Peptides; D011994:Recombinant Proteins; C577913:poly(2-oxazoline); D003094:Collagen; D017364:Collagenases; D020780:Matrix Metalloproteinase 9; D006909:Hydroxyproline
keywords: antimicrobial; collagenase inhibitors; dental adhesives; notched-edge; polyoxazoline; self-etch