title: [Progress of improving blood donor screening by nucleic acid technology].
abstract: With increasing application of blood transfusion, the research of side-effects such as transfusion-transmitted infections (TTIs) became more and more important. Up to the 90's of the 20th century, the first blood donor screening for pathogens transfected from blood transfusion entirely depended on serological test. At this time, the detection of virus were performed mainly by using method of detecting antibody, except hepatitis B virus (HBV) can be detected by hepatitis B surface antigen (HBsAg). Now, the molecular technologies, such as the polymerase chain reaction (PCR), have been used in clinic. These technologic methods can provide capability of detection for blood donor screening and reduced possibility of infection from blood transfusion. This review summarises the development of nucleic acid amplification technology and describes its current state.
authors.affiliation: Department of Hematology and Oncology, Zhongda Hospital,Medical School, Southeast University, Nanjing, 210009, Jiangsu Province, China; Department of Clinical Laboratory, Jiangsu Province Blood Center, Nanjing 210042, Jiangsu Province, China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Cai
authors.affiliation: Department of Hematology and Oncology, Zhongda Hospital,Medical School, Southeast University, Nanjing, 210009, Jiangsu Province, China. E-mail:cba8888@hotmail.com;E-mail: bachen@seu.edu.cn.
authors.forename: Bao-An
authors.initials: BA
authors.lastname: Chen
mesh_terms: D004279:DNA, Viral / Q000097:blood; D046148:Donor Selection; D006801:Humans; D008403:Mass Screening; D021141:Nucleic Acid Amplification Techniques
chemical_list: D004279:DNA, Viral