title: Quantitative comparison and metabolite profiling of saponins in different parts of the root of Panax notoginseng.
abstract: Although both rhizome and root of Panax notoginseng are officially utilized as notoginseng in "Chinese Pharmacopoeia", individual parts of the root were differently used in practice. To provide chemical evidence for the differentiated usage, quantitative comparison and metabolite profiling of different portions derived from the whole root, as well as commercial samples, were carried out, showing an overall higher content of saponins in rhizome, followed by main root, branch root, and fibrous root. Ginsenoside Rb2 was proposed as a potential marker with a content of 0.5 mg/g as a threshold value for differentiating rhizome from other parts. Multivariate analysis of the metabolite profile further suggested 32 saponins as potential markers for the discrimination of different parts of notoginseng. Collectively, the study provided comprehensive chemical evidence for the distinct usage of different parts of notoginseng and, hence, is of great importance for the rational application and exploitation of individual parts of notoginseng.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology , Macau, China.
authors.forename: Jing-Rong
authors.initials: JR
authors.lastname: Wang
authors.forename: Lee-Fong
authors.initials: LF
authors.lastname: Yau
authors.forename: Wei-Na
authors.initials: WN
authors.lastname: Gao
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Pui-Wing
authors.initials: PW
authors.lastname: Yick
authors.forename: Liang
authors.initials: L
authors.lastname: Liu
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Jiang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D036145:Ginsenosides / Q000032:analysis; D055432:Metabolomics; D053723:Panax notoginseng / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000032:analysis*
chemical_list: D036145:Ginsenosides; D012503:Saponins; C048026:ginsenoside Rb2
keywords: ginsenosides, LC−MS; metabolomics; notoginseng; root