title: [The effect of NALP3 inflammasome pathway in visceral adipose tissue on the development of insulin resistance in catch-up growth after food restriction in rats].
abstract: OBJECTIVE
  To investigate the effects of catch-up growth (CUG) on the natch domain, leucine-rich repeat and PVD-containing protein 3 (NALP3) inflammasome pathway in visceral adipose tissue (VAT) and the mechanism of insulin resistance (IR) in CUG.
  
  
  METHODS
  Sprague-Dawley rats were randomly divided into the normal chow (NC) and the catch-up growth group (CUG). General characteristics, glucose infusion rate60-120 (GIR60-120) in hyperinsulinemic-euglycemic clamp and expression of NALP3 inflammasome, caspase1(p10) and IL-1β (p17) cleavage in VAT were respectively examined on week 4, 6 and 8 of the experiment.
  
  
  RESULTS
  After 4-week food restriction, lower percentage of abdominal fat mass (AFM%) was presented in the CUG group than the NC group [(11.54 ± 1.81)% vs (7.72 ± 1.47)%, P < 0.05]. In the CUG group, decreased expression of NALP3 inflammasome, caspase1 (p10) and IL-1β (p17) cleavage in VAT were found (0.47 ± 0.03 vs 0.28 ± 0.04, P < 0.01; 0.30 ± 0.02 vs 0.20 ± 0.03, P < 0.01; 0.52 ± 0.04 vs 0.37 ± 0.04, P < 0.05; respectively), whereas GIR60-120 was slightly improved [(23.47 ± 0.89) mg×min(-1)×kg(-1) vs (25.34 ± 1.16) mg×min(-1)×kg(-1), P > 0.05]. After refeeding, AFM% and the expression of NALP3 inflammasome, caspase1 (p10) and IL-1β (p17) cleavage in VAT in CUG group were shown to be increased with the time. Concomitant with those changes, GIR60-120 was gradually impaired. On week 4 of refeeding, AFM% and the expression of NALP3 inflammasome, caspase1 (p10) and IL-1β (p17) cleavage in VAT were significantly increased in the CUG group compared with the NC group [(12.52 ± 0.64)% vs (15.16 ± 1.10)%, P < 0.01; 0.52 ± 0.02 vs 0.65 ± 0.05, P < 0.05; 0.33 ± 0.03 vs 0.54 ± 0.02, P < 0.01; 0.55 ± 0.04 vs 0.65 ± 0.05, P < 0.05; respectively], while GIR60-120 was significantly attenuated [(21.45 ± 1.20) mg×min(-1)×kg(-1) vs (14.27 ± 1.06) mg×min(-1)×kg(-1), P < 0.05]. Correlation analysis showed that the expression of NALP3 and caspase1 (p10) in VAT were positively correlated with AFM% (r = 0.946, P < 0.01; r = 0.922, P < 0.01), while negatively correlated with GIR60-120 (r = -0.902, P < 0.01; r = -0.944, P < 0.01).
  
  
  CONCLUSIONS
  NALP3 inflammasome pathway in VAT is notably activated during CUG, which may contribute to the etiology of IR in CUG.
authors.affiliation: Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. Email: cheria_chen@126.com.
authors.forename: Lulu
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Yu
authors.affiliation: Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: Huiqing
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: Tianshu
authors.initials: T
authors.lastname: Zeng
authors.affiliation: Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: Wenfang
authors.initials: W
authors.lastname: Xia
authors.affiliation: Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: Jiaoyue
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002352:Carrier Proteins; D005947:Glucose; D058847:Inflammasomes; D007333:Insulin Resistance; D053583:Interleukin-1beta; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D051381:Rats; D017207:Rats, Sprague-Dawley; D018160:Receptors, Cytoplasmic and Nuclear / Q000502:physiology*
chemical_list: D002352:Carrier Proteins; D058847:Inflammasomes; D053583:Interleukin-1beta; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C573346:Nlrp3 protein, rat; D018160:Receptors, Cytoplasmic and Nuclear; D005947:Glucose