title: Circulating levels of chemokines in psoriasis.
abstract: Chemokines may contribute to local and systemic inflammation in patients with psoriasis. Previous studies have demonstrated the importance of chemokine ligands and receptors in the recruitment of T cells into psoriatic lesional skin and synovial fluid. The aim of this study was to evaluate the levels of Th1-related chemokines in psoriasis and to investigate any association with disease severity. We quantified serum levels of CXCL9, CXCL10 and CXCL16 and the frequencies of CD4+CXCR3+ T lymphocytes through ELISA and flow cytometry, respectively. A total of 38 patients with psoriasis and 33 controls were included. There were no significant differences in chemokine levels between psoriasis and control groups. Patients with psoriatic arthritis had lower median level of CXCL10 when compared with controls (p=0.03). There were no significant correlations between serum chemokines analyzed and disease severity. Frequencies of CD4+CXCR3+ T cells were lower in patients with psoriasis than in controls (p<0.01). A sensitivity analysis excluding patients on systemic therapy yielded similar results. Serum concentrations of CXCL9, CXCL10 and CXCL16 were not increased in the psoriasis group or correlated with disease severity. Systemic levels of chemokine ligands do not seem to be sensitive biomarkers of disease activity or accurate parameters to predict response to therapy. Frequencies of CD4+CXCR3+ T cells were decreased in the peripheral blood of psoriasis patients, possibly due to recruitment to inflammatory lesions.
authors.affiliation: Department of Clinical Pathology, University of Campinas (UNICAMP) , Campinas, São Paulo , Brazil and.
authors.forename: Xinaida Taligare
authors.initials: XT
authors.lastname: Lima
authors.forename: Rômulo Tadeu Dias
authors.initials: RT
authors.lastname: Oliveira
authors.forename: Fernanda Gambogi
authors.initials: FG
authors.lastname: Braga
authors.forename: Renata Ferreira
authors.initials: RF
authors.lastname: Magalhães
authors.forename: Ronei Luciano
authors.initials: RL
authors.lastname: Mamoni
authors.forename: Maria Heloisa Souza Lima
authors.initials: MH
authors.lastname: Blotta
mesh_terms: D000293:Adolescent; D000328:Adult; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D054357:Chemokine CXCL10 / Q000097:blood; D000075743:Chemokine CXCL16; D054370:Chemokine CXCL9 / Q000097:blood; D019743:Chemokines, CXC / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000097:blood* / Q000276:immunology / Q000473:pathology; D054367:Receptors, CXCR3 / Q000097:blood; D051116:Receptors, Scavenger / Q000097:blood; D012720:Severity of Illness Index
chemical_list: C114753:CXCL10 protein, human; C423727:CXCL16 protein, human; C080810:CXCL9 protein, human; C515187:CXCR3 protein, human; D054357:Chemokine CXCL10; D000075743:Chemokine CXCL16; D054370:Chemokine CXCL9; D019743:Chemokines, CXC; D054367:Receptors, CXCR3; D051116:Receptors, Scavenger
keywords: CXCL10; CXCL16; CXCL9; CXCR3; Chemokines; psoriasis