title: Expression of Gli1 and Wnt2B correlates with progression and clinical outcome of pancreatic cancer.
abstract: OBJECTIVE
  The aim of this study was to investigate the expression and clinical significance of Gli1 and Wnt2B in pancreatic cancer.
  
  
  METHODS
  We have constructed a formalin-fixed paraffin embedded pancreatic tissue microarrays 180 cylindrical tissue cores of human pancreatic cancer and its paracancerous nonmalignant pancreatic specimens (NMPs) from 90 patients. Levels of Gli1 and Wnt2B were measured by immunohistochemistry. We analyzed the correlations between the expression of these factors and clinicopathological parameters including prognosis.
  
  
  RESULTS
  The expressions of both Gli1 and Wnt2B in human pancreatic cancer tissues were significantly higher than those of normal pancreatic tissues (P=0.000, P=0.004 respectively). The analysis showed that the high cytoplasmic expression levels of Gli1 in pancreatic cancer tissues had significant correlation with lymph node metastasis (P=0.036) and Wnt2B had significant correlation with perineural invasion (P=0.045). Gli1 and Wnt2B have no positive correlation. Survival analysis by Kaplan-Meier demonstrated that elevated Wnt2B expression in cancer tissue predicted worse overall survival (OS) compared with group in lower expression (P=0.024). No correlation was found between the expression of Gli1 and overall survival of pancreatic cancer patients (P>0.05).
  
  
  CONCLUSIONS
  In conclusion, these results indicate that the high-expression levels of Gli1 and Wnt2B might play a pivotal role during tumorigenesis of pancreatic cancer, and the high expression of Wnt2B might be associated with poor prognosis.
authors.affiliation: Department of Geriatrics, East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Geriatrics, East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Fengru
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Institute of Digestive Diseases, Tongji Hospital, Tongji University School of Medicine Shanghai 200065, China.
authors.forename: Chengzhi
authors.initials: C
authors.lastname: He
authors.affiliation: Department of Intensive Care Unit, East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Xuebin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Geriatrics, East Hospital, Tongji University School of Medicine Shanghai 200120, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Institute of Digestive Diseases, Tongji Hospital, Tongji University School of Medicine Shanghai 200065, China ; National Engineering Center for Biochip at Shanghai Shanghai 201203, China.
authors.forename: Hengjun
authors.initials: H
authors.lastname: Gao
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D018450:Disease Progression; D005260:Female; D006023:Glycoproteins / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D011379:Prognosis; D046888:Tissue Array Analysis; D014157:Transcription Factors / Q000032:analysis / Q000096:biosynthesis*; D051153:Wnt Proteins / Q000032:analysis / Q000096:biosynthesis*; D000071676:Zinc Finger Protein GLI1
chemical_list: D014408:Biomarkers, Tumor; C400551:GLI1 protein, human; D006023:Glycoproteins; D014157:Transcription Factors; C115496:WNT2B protein, human; D051153:Wnt Proteins; D000071676:Zinc Finger Protein GLI1
keywords: Gli1; Pancreatic cancer; Wnt2B; prognosis