title: Genetic alterations of chromosome 8 genes in oral cancer.
abstract: The clinical relevance of DNA copy number alterations in chromosome 8 were investigated in oral cancers. The copy numbers of 30 selected genes in 33 OSCC patients were detected using the multiplex ligation-dependent probe amplification (MLPA) technique. Amplifications of the EIF3E gene were found in 27.3% of the patients, MYC in 18.2%, RECQL4 in 15.2% and MYBL1 in 12.1% of patients. The most frequent gene losses found were the GATA4 gene (24.2%), FGFR1 gene (24.2%), MSRA (21.2) and CSGALNACT1 (12.1%). The co-amplification of EIF3E and RECQL4 was found in 9% of patients and showed significant association with alcohol drinkers. There was a significant association between the amplification of EIF3E gene with non-betel quid chewers and the negative lymph node status. EIF3E amplifications did not show prognostic significance on survival. Our results suggest that EIF3E may have a role in the carcinogenesis of OSCC in non-betel quid chewers.
authors.affiliation: 1] Department of Oro-Maxillofacial Surgical and Medical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia [2] Oral Cancer Research &Coordinating Centre (OCRCC), Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Zachary Wei Ern
authors.initials: ZW
authors.lastname: Yong
authors.affiliation: 1] Department of Oro-Maxillofacial Surgical and Medical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia [2] Oral Cancer Research &Coordinating Centre (OCRCC), Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Zuraiza Mohamad
authors.initials: ZM
authors.lastname: Zaini
authors.affiliation: 1] Department of Oro-Maxillofacial Surgical and Medical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia [2] Oral Cancer Research &Coordinating Centre (OCRCC), Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Thomas George
authors.initials: TG
authors.lastname: Kallarakkal
authors.affiliation: Oral Cancer Research &Coordinating Centre (OCRCC), Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Lee Peng
authors.initials: LP
authors.lastname: Karen-Ng
authors.affiliation: 1] Department of Oro-Maxillofacial Surgical and Medical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia [2] Oral Cancer Research &Coordinating Centre (OCRCC), Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Zainal Ariff Abdul
authors.initials: ZA
authors.lastname: Rahman
authors.affiliation: 1] Department of Oro-Maxillofacial Surgical and Medical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia [2] Oral Cancer Research &Coordinating Centre (OCRCC), Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Siti Mazlipah
authors.initials: SM
authors.lastname: Ismail
authors.affiliation: Department of Pathology, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur.
authors.forename: Noor Akmal
authors.initials: NA
authors.lastname: Sharifah
authors.affiliation: Department of Oral Surgery, Hospital Kuala Lumpur, 50588 Kuala Lumpur, Malaysia.
authors.forename: Wan Mahadzir Wan
authors.initials: WM
authors.lastname: Mustafa
authors.affiliation: Department of Oral and Maxillofacial Surgery, Hospital Tengku Ampuan Rahimah, Klang, Malaysia.
authors.forename: Mannil Thomas
authors.initials: MT
authors.lastname: Abraham
authors.affiliation: Oral Surgery Clinic, Hospital Umum Sarawak, Kuching, Malaysia.
authors.forename: Keng Kiong
authors.initials: KK
authors.lastname: Tay
authors.affiliation: 1] Department of Oro-Maxillofacial Surgical and Medical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia [2] Oral Cancer Research &Coordinating Centre (OCRCC), Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Rosnah Binti
authors.initials: RB
authors.lastname: Zain
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002869:Chromosome Aberrations; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D056915:DNA Copy Number Variations / Q000235:genetics*; D005260:Female; D050991:GATA4 Transcription Factor / Q000235:genetics; D018628:Gene Dosage / Q000235:genetics*; D006801:Humans; D008297:Male; D051150:Methionine Sulfoxide Reductases / Q000235:genetics; D009062:Mouth Neoplasms / Q000235:genetics*; D017350:N-Acetylgalactosaminyltransferases / Q000235:genetics; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000235:genetics
chemical_list: D050991:GATA4 Transcription Factor; C484170:GATA4 protein, human; D051150:Methionine Sulfoxide Reductases; C027219:methionine sulfoxide reductase; D017350:N-Acetylgalactosaminyltransferases; C472559:chondroitin sulfate N-acetylgalactosaminyltransferase-1; C496348:FGFR1 protein, human; D051496:Receptor, Fibroblast Growth Factor, Type 1