title: Comparative role of 20% cord blood serum and 20% autologous serum in dry eye associated with Hansen's disease: a tear proteomic study.
abstract: BACKGROUND
  To compare the role of topically applied serum therapy with preservative-free artificial tear (AT) drops in patients with moderate to severe dry eye in Hansen's disease along with change in tear protein profile.
  
  
  METHODS
  144 consecutive patients were randomly divided into three groups. After a baseline examination of clinical parameters, each of the patients received designated modality of topical therapy six times a day for 6 weeks. Post-treatment documentation of clinical parameters was done at 6 weeks, and then at 12 weeks after discontinuation of topical therapy. Analysis of three tear proteins using gel electrophoresis (sodium dodecyl sulfate polyacrylamide gel electrophoresis) was done at baseline, at the first and second post-treatment visits.
  
  
  RESULTS
  In the cord blood serum (CBS) group, except for McMonnies score and staining score, all other clinical parameters showed continued improvement in the first and second post-treatment analyses. In the autologous serum (ALS) group, all the clinical parameters except Schirmer's I showed significant improvement in the first post-treatment analysis .This was sustained at a significant level in the second analysis except for tear film break-up time (TBUT) and conjunctival impression cytology grading. In the AT group, all the parameters improved at a non-significant level except for TBUT in the first analysis. In the next analysis, apart from McMonnies score and TBUT, other clinical parameters did not improve. In the ALS and CBS groups, tear lysozyme, lactoferrin levels improved in both post-treatment measurements (statistically insignificant).Total tear protein continued to increase at statistically significant levels in the first and second post-treatment analyses in the CBS group and at a statistically insignificant level in the ALS group. In the AT group, the three tear proteins continued to decrease in both the analyses.
  
  
  CONCLUSIONS
  In moderate to severe dry eye in Hansen's disease, serum therapy in comparison with AT drops, improves clinical parameters and causes betterment in tear protein profile.
  
  
  BACKGROUND
  CTRI/2013/07/003802.
authors.affiliation: Department of Ophthalmology, Nil Ratan Sircar (NRS) Medical College and Hospital, Kolkata, West Bengal, India.
authors.forename: Somnath
authors.initials: S
authors.lastname: Mukhopadhyay
authors.affiliation: Cornea and Refractive Surgery Services, Regional Institute of Ophthalmology (RIO), Kolkata, West Bengal, India.
authors.forename: Swarnali
authors.initials: S
authors.lastname: Sen
authors.affiliation: Cornea and Refractive Surgery Services, Regional Institute of Ophthalmology (RIO), Kolkata, West Bengal, India.
authors.forename: Himadri
authors.initials: H
authors.lastname: Datta
mesh_terms: D000287:Administration, Topical; D000328:Adult; D000368:Aged; D003228:Conjunctiva / Q000473:pathology; D004311:Double-Blind Method; D015352:Dry Eye Syndromes / Q000378:metabolism / Q000382:microbiology / Q000628:therapy*; D004591:Electrophoresis, Polyacrylamide Gel; D015818:Eye Infections, Bacterial / Q000378:metabolism / Q000382:microbiology / Q000628:therapy*; D005136:Eye Proteins / Q000378:metabolism*; D005260:Female; D005312:Fetal Blood; D006801:Humans; D007781:Lactoferrin / Q000378:metabolism; D007918:Leprosy / Q000378:metabolism / Q000382:microbiology / Q000628:therapy*; D008297:Male; D008875:Middle Aged; D009113:Muramidase / Q000378:metabolism; D011446:Prospective Studies; D040901:Proteomics; D044967:Serum; D013666:Tears / Q000737:chemistry
chemical_list: D005136:Eye Proteins; C042434:tear proteins; D009113:Muramidase; D007781:Lactoferrin
keywords: Clinical Trial; Conjunctiva; Cornea; Ocular surface; Tears