title: Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil.
abstract: OBJECTIVE
  To characterize bone marrow progenitors cells grown in vitro, using native goats from northeastern Brazil as animal model.
  
  
  METHODS
  Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers - Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog).
  
  
  RESULTS
  Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 106 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog.
  
  
  CONCLUSIONS
  Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied.
authors.affiliation: School of Veterinary Medicine, UFPI, Teresina, PI, Brazil.
authors.forename: Osmar Ferreira da
authors.initials: OF
authors.lastname: Silva Filho
authors.affiliation: Department of Animal Science, School of Veterinary Medicine, UFPI, Bom Jesus, PI, Brazil.
authors.forename: Napoleão Martins
authors.initials: NM
authors.lastname: Argôlo Neto
authors.affiliation: Department of Veterinary Morphophysiology, School of Veterinary Medicine, UFPI, Teresina, PI, Brazil.
authors.forename: Maria Acelina Martins de
authors.initials: MA
authors.lastname: Carvalho
authors.affiliation: UFPI, Teresina, PI, Brazil.
authors.forename: Yulla Klinger de
authors.initials: YK
authors.lastname: Carvalho
authors.affiliation: Department of Animal Science, State University of Piaui, Uniao, PI, Brazil.
authors.forename: Anaemilia das Neves
authors.initials: Ad
authors.lastname: Diniz
authors.affiliation: School of Veterinary Medicine, UFPI, Teresina, PI, Brazil.
authors.forename: Laécio da Silva
authors.initials: Lda S
authors.lastname: Moura
authors.affiliation: Department of Basic Sciences, Faculty of Animal Sciences and Food Engineering, Sao Paulo University, Pirassununga, SP, Brazil.
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: Ambrósio
authors.affiliation: Teaching and Research Institute, Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil.
authors.forename: Janaína Munuera
authors.initials: JM
authors.lastname: Monteiro
authors.affiliation: Department of Animal Science, UESPI, Uniao, PI, Brazil.
authors.forename: Hatawa Melo de
authors.initials: HM
authors.lastname: Almeida
authors.affiliation: Department of Surgery, School of Veterinary, USP, Sao Paulo, SP, Brazil.
authors.forename: Maria Angélica
authors.initials: MA
authors.lastname: Miglino
authors.affiliation: Federal University of Ceara, Fortaleza, CE, Brazil.
authors.forename: Jacyara de Jesus Rosa Pereira
authors.initials: Jde J
authors.lastname: Alves
authors.affiliation: UFPI, Teresina, PI, Brazil.
authors.forename: Kássio Vieira
authors.initials: KV
authors.lastname: Macedo
authors.affiliation: School of Veterinary Medicine, UFPI, Teresina, PI, Brazil.
authors.forename: Andressa Rego da
authors.initials: AR
authors.lastname: Rocha
authors.affiliation: UFPI, Teresina, PI, Brazil.
authors.forename: Matheus Levi Tajra
authors.initials: ML
authors.lastname: Feitosa
authors.affiliation: Department of Veterinary Morphophysiology, UFPI, Teresina, PI, Brazil.
authors.forename: Flávio Ribeiro
authors.initials: FR
authors.lastname: Alves
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D001938:Brazil; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006041:Goats; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D023421:Models, Animal; D050814:Octamer Transcription Factor-3 / Q000302:isolation & purification; D018809:Proliferating Cell Nuclear Antigen / Q000302:isolation & purification; D014746:Vimentin / Q000302:isolation & purification
chemical_list: D050814:Octamer Transcription Factor-3; D018809:Proliferating Cell Nuclear Antigen; D014746:Vimentin