title: Over-expression of testis-specific expressed gene 1 attenuates the proliferation and induces apoptosis of GC-1spg cells.
abstract: The effects of over-expression of testis-specific expressed gene 1 (TSEG-1) on the viability and apoptosis of cultured spermatogonial GC-1spg cells were investigated, and the immortal spermatogonial cell line GC-1spg (CRL-2053™) was obtained as the cell model in order to explore the function of TSEG-1. We transfected the eukaryotic vector of TSEG-1, named as pEGFP-TSEG-1 into cultured spermatogonial GC-1spg cells. Over-expression of TSEG-1 inhibited the proliferation of GC-1spg cells, and arrested cell cycle slightly at G0/G1 phase. Transfection of TSEG-1 attenuated the transcript levels of Ki-67, PCNA and cyclin D1. In addition, over-expression of TSEG-1 induced early and late apoptosis, and reduced the mitochondrial membrane potential of GC-1spg cells. Moreover, transfection of TSEG-1 significantly enhanced the ratio of Bax/Bcl-2 and transcript levels of caspase 9, and decreased the expression of Fas and caspase 8 in GC-1spg cells. These results indicated over-expression of TSEG-1 suppresses the proliferation and induces the apoptosis of GC-1spg cells, which establishes a basis for further study on the function of TSEG-1.
authors.affiliation: First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450052, China.|Department of Surgery, Huazhong University of Science and Technology, Wuhan, 430022, China.|Human Disease Related Gene Research Group, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Chao-Hui
authors.initials: CH
authors.lastname: Gu
authors.affiliation: First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450052, China.
authors.forename: Feng-Yan
authors.initials: FY
authors.lastname: Tian
authors.affiliation: Department of Surgery, Huazhong University of Science and Technology, Wuhan, 430022, China.|Human Disease Related Gene Research Group, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Jia-Rui
authors.initials: JR
authors.lastname: Pu
authors.affiliation: Department of Pathology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Li-Duan
authors.initials: LD
authors.lastname: Zheng
authors.affiliation: Department of Surgery, Huazhong University of Science and Technology, Wuhan, 430022, China.|Human Disease Related Gene Research Group, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Mei
authors.affiliation: Department of Surgery, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Fu-Qing
authors.initials: FQ
authors.lastname: Zeng
authors.affiliation: First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450052, China.
authors.forename: Jin-Jian
authors.initials: JJ
authors.lastname: Yang
authors.affiliation: First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450052, China.
authors.forename: Quan-Cheng
authors.initials: QC
authors.lastname: Kan
authors.affiliation: Department of Surgery, Huazhong University of Science and Technology, Wuhan, 430022, China. zgwhgch@hotmail.com.|Human Disease Related Gene Research Group, Huazhong University of Science and Technology, Wuhan, 430022, China. zgwhgch@hotmail.com.|Department of Pathology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. zgwhgch@hotmail.com.
authors.forename: Qiang-Song
authors.initials: QS
authors.lastname: Tong
mesh_terms: D000818:Animals; D053181:Caspase 8 / Q000096:biosynthesis / Q000235:genetics; D002460:Cell Line; D019938:Cyclin D1 / Q000096:biosynthesis / Q000235:genetics; D016193:G1 Phase / Q000502:physiology*; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D019394:Ki-67 Antigen / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D051379:Mice; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis / Q000235:genetics; D016192:Resting Phase, Cell Cycle / Q000502:physiology*; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis / Q000235:genetics
chemical_list: C494827:Bax protein, mouse; C530105:Ccnd1 protein, mouse; D006657:Histones; D019394:Ki-67 Antigen; C498355:Mki67 protein, mouse; D018809:Proliferating Cell Nuclear Antigen; D051028:bcl-2-Associated X Protein; D019938:Cyclin D1; C505526:Casp8 protein, mouse; D053181:Caspase 8