title: Expression of myotilin during chicken development.
abstract: Several missense mutations in the Z-band protein, myotilin, have been implicated in human muscle diseases such as myofibrillar myopathy, spheroid body myopathy, and distal myopathy. Recently, we have reported the cloning of chicken myotilin cDNA. In this study, we have investigated the expression of myotilin in cross-striated muscles from developing chicken by qRT-PCR and in situ hybridizations. In situ hybridization of embryonic stages shows myotilin gene expression in heart, somites, neural tissue, eyes and otocysts. RT-PCR and qRT-PCR data, together with in situ hybridization results point to a biphasic transcriptional pattern for MYOT gene during early heart development with maximum expression level in the adult. In skeletal muscle, the expression level starts decreasing after embryonic day 20 and declines in the adult skeletal muscles. Western blot assays of myotilin in adult skeletal muscle reveal a decrease in myotilin protein compared with levels in embryonic skeletal muscle. Our results suggest that MYOT gene may undergo transcriptional activation and repression that varies between tissues in developing chicken. We believe this is the first report of the developmental regulation on myotilin expression in non-mammalian species.
authors.affiliation: Department of Medicine, SUNY Upstate Medical University, Syracuse, New York.
authors.forename: Dipak K
authors.initials: DK
authors.lastname: Dube
authors.forename: Jushuo
authors.initials: J
authors.lastname: Wang
authors.forename: Christopher
authors.initials: C
authors.lastname: Pellenz
authors.forename: Yingli
authors.initials: Y
authors.lastname: Fan
authors.forename: Syamalima
authors.initials: S
authors.lastname: Dube
authors.forename: Mingda
authors.initials: M
authors.lastname: Han
authors.forename: Kersti
authors.initials: K
authors.lastname: Linask
authors.forename: Jean M
authors.initials: JM
authors.lastname: Sanger
authors.forename: Joseph W
authors.initials: JW
authors.lastname: Sanger
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D064211:Connectin / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology*; D018482:Muscle, Skeletal / Q000196:embryology / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D064211:Connectin; D012333:RNA, Messenger
keywords: Z-bands; chick embryos; heart; myotilin; skeletal muscle