title: First report of the symbiotic bacterium Xenorhabdus indica associated with the entomopathogenic nematode Steinernema yirgalemense.
abstract: The entomopathogenic nematode Steinernema yirgalemense is considered a promising agent in the biocontrol of insects. However, little is known about the bacteria living in symbiosis with the nematode. In this study, we have identified the only available bacterial strain (157-C) isolated from S. yirgalemense, as a member of the species Xenorhabdus indica. Identification was based on 16S rDNA, recA, dnaN, gltX, gyrB and infB gene sequence analyses. The relatedness of strain 157-C to the type strain of X. indica (DSM 17 382) was confirmed with DNA-DNA hybridization. The phenotypic characteristics of strain 157-C are similar to those described for the type strain of X. indica. This is the first report associating X. indica with S. yirgalemense.
authors.affiliation: Department of Conservation Ecology and Entomology,Stellenbosch University, Private Bag X1,7602Matieland,Stellenbosch,South Africa.
authors.forename: T
authors.initials: T
authors.lastname: Ferreira
authors.affiliation: Department of Microbiology,Stellenbosch University, Private Bag X1,7602Matieland,Stellenbosch,South Africa.
authors.forename: C A
authors.initials: CA
authors.lastname: van Reenen
authors.affiliation: Université Montpellier 2, UMR1333 Diversité, Génomes & Interactions Microorganismes-Insectes (DGIMI),F-34000Montpellier,France.
authors.forename: P
authors.initials: P
authors.lastname: Tailliez
authors.affiliation: Université Montpellier 2, UMR1333 Diversité, Génomes & Interactions Microorganismes-Insectes (DGIMI),F-34000Montpellier,France.
authors.forename: S
authors.initials: S
authors.lastname: Pagès
authors.affiliation: Department of Conservation Ecology and Entomology,Stellenbosch University, Private Bag X1,7602Matieland,Stellenbosch,South Africa.
authors.forename: A P
authors.initials: AP
authors.lastname: Malan
authors.affiliation: Department of Microbiology,Stellenbosch University, Private Bag X1,7602Matieland,Stellenbosch,South Africa.
authors.forename: L M T
authors.initials: LM
authors.lastname: Dicks
mesh_terms: D000818:Animals; D008969:Molecular Sequence Data; D009036:Moths / Q000469:parasitology*; D010802:Phylogeny; D017168:Rhabditida / Q000382:microbiology* / Q000502:physiology; D013559:Symbiosis; D020593:Xenorhabdus / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*