title: Identification of cytidine 2',3'-cyclic monophosphate and uridine 2',3'-cyclic monophosphate in Pseudomonas fluorescens pfo-1 culture.
abstract: Cytidine 2',3'-cyclic monophosphate (2',3'-cCMP) and uridine 2',3'-cyclic monophosphate (2',3'-cUMP) were isolated from Pseudomonas fluorescens pfo-1 cell extracts by semi-preparative reverse phase HPLC. The structures of the two compounds were confirmed by NMR and mass spectroscopy against commercially available authentic samples. Concentrations of both intracellular and extracellular 2',3'-cCMP and 2',3'-cUMP were determined. Addition of 2',3'-cCMP and 2',3'-cUMP to P. fluorescens pfo-1 culture did not significantly affect the level of biofilm formation in static liquid cultures.
authors.affiliation: Department of Chemistry, Brock University, 500 Glenridge Ave., St. Catharines, Ontario L2S 3A1, Canada.
authors.forename: Emily
authors.initials: E
authors.lastname: Bordeleau
authors.affiliation: Department of Chemistry, Brock University, 500 Glenridge Ave., St. Catharines, Ontario L2S 3A1, Canada.
authors.forename: Christopher
authors.initials: C
authors.lastname: Oberc
authors.affiliation: Department of Chemistry, Brock University, 500 Glenridge Ave., St. Catharines, Ontario L2S 3A1, Canada.
authors.forename: Eve
authors.initials: E
authors.lastname: Ameen
authors.affiliation: Department of Chemistry, Brock University, 500 Glenridge Ave., St. Catharines, Ontario L2S 3A1, Canada.
authors.forename: Amanda Mendes
authors.initials: AM
authors.lastname: da Silva
authors.affiliation: Department of Chemistry, Brock University, 500 Glenridge Ave., St. Catharines, Ontario L2S 3A1, Canada.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Yan
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003597:Cytosine Nucleotides / Q000737:chemistry* / Q000302:isolation & purification; D009712:Nucleotides, Cyclic / Q000737:chemistry* / Q000302:isolation & purification; D011551:Pseudomonas fluorescens / Q000737:chemistry* / Q000166:cytology; D014542:Uridine Monophosphate / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D003597:Cytosine Nucleotides; D009712:Nucleotides, Cyclic; C045338:cyclic 3',5'-uridine monophosphate; C019310:cifostodine; D014542:Uridine Monophosphate
keywords: Cyclic nucleoside monophosphate; HPLC; Pseudomonas fluorescens pfo-1; cCMP; cUMP