title: Brazilian green propolis improves immune function in aged mice.
abstract: Aging weakened innate and adaptive immunity both quantitatively and qualitatively. Some components in propolis could stimulate immune function in young animals or cultured immune cells in vitro. Few studies had been carried out in the aged. The present study was to evaluate the effects of Brazilian green propolis supplementation on the immunological parameters in aged mice. Eighty Kunming mice, aged 15-18 months, were randomly assigned to the control and three experimental groups supplemented with different doses (83.3, 157.4 and 352.9 mg/kg.bw respectively) of Brazilian green propolis. The experiment lasted for 4 weeks. Contents of total polyphenol, flavonoid, cinnamic acid and artepillin-C in Brazilian green propolis were analyzed. Splenic NK cytotoxic, T lymphocyte proliferation and antibody generation cells, as well as the phagocytosis of peritoneal macrophages, ear swelling, and serum contents of IgG, IgM, hemolysin and cytokines were measured. After 4 weeks of treatment, the phagocytosis of peritoneal macrophages was enhanced in 157.4 mg/kg and 352.9 mg/kg groups. Ear swelling increased in all propolis treatmented groups. Antibodies specific to sheep erythrocytes were higher in the groups receiving 157.4 and 352.9 mg/kg.bw than that of control group. IgG level dramatically increased in the groups receiving 83.3 and 157.4 mg/kg.bw in comparison to the control group. These results indicate that administration of Brazilian green propolis have a positive effect on innate and adaptive immunity in aged mice.
authors.affiliation: Institute of Health and Environmental Medicine, Tianjin, 300050, China.
authors.forename: Weina
authors.initials: W
authors.lastname: Gao
authors.affiliation: Institute of Health and Environmental Medicine, Tianjin, 300050, China.
authors.forename: Jianquan
authors.initials: J
authors.lastname: Wu
authors.affiliation: Institute of Health and Environmental Medicine, Tianjin, 300050, China.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Wei
authors.affiliation: Institute of Health and Environmental Medicine, Tianjin, 300050, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Pu
authors.affiliation: Institute of Health and Environmental Medicine, Tianjin, 300050, China.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Guo
authors.affiliation: Institute of Health and Environmental Medicine, Tianjin, 300050, China.
authors.forename: Jijun
authors.initials: J
authors.lastname: Yang
authors.affiliation: By-Health Nutrition and Health Research Center, 510620, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.affiliation: By-Health Nutrition and Health Research Center, 510620, China.
authors.forename: Haiji
authors.initials: H
authors.lastname: Luo
keywords: aged mice; immune function; propolis