title: Genome-wide DNA binding pattern of the homeodomain transcription factor Sine oculis (So) in the developing eye ofDrosophila melanogaster.
abstract: The eye of the fruit flyDrosophila melanogasterprovides a highly tractable genetic model system for the study of animal development, and many genes that regulateDrosophilaeye formation have homologs implicated in human development and disease. Among these is the homeobox genesine oculis(so), which encodes a homeodomain transcription factor (TF) that is both necessary for eye development and sufficient to reprogram a subset of cells outside the normal eye field toward an eye fate. We have performed a genome-wide analysis of So binding to DNA prepared from developingDrosophilaeye tissue in order to identify candidate direct targets of So-mediated transcriptional regulation, as described in our recent article [1]. The data are available from NCBI Gene Expression Omnibus (GEO) with the accession number GSE52943. Here we describe the methods, data analysis, and quality control of our So ChIP-seq dataset.
authors.affiliation: Program in Developmental Biology, Baylor College of Medicine, Houston, Texas, United States of America.
authors.forename: Barbara
authors.initials: B
authors.lastname: Jusiak
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America ; Human Genome Sequencing Center, Baylor College of Medicine, Houston, Texas, United States of America.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas, United States of America.
authors.forename: Umesh C
authors.initials: UC
authors.lastname: Karandikar
authors.affiliation: Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas, United States of America.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kwak
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America ; Human Genome Sequencing Center, Baylor College of Medicine, Houston, Texas, United States of America.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Program in Developmental Biology, Baylor College of Medicine, Houston, Texas, United States of America ; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America ; Human Genome Sequencing Center, Baylor College of Medicine, Houston, Texas, United States of America.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.affiliation: Program in Developmental Biology, Baylor College of Medicine, Houston, Texas, United States of America ; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America ; Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas, United States of America ; Department of Neuroscience, Baylor College of Medicine, Houston, Texas, United States of America ; Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, United States of America ; Program in Cell and Molecular Biology, Baylor College of Medicine, Houston, Texas, United States of America.
authors.forename: Graeme
authors.initials: G
authors.lastname: Mardon
keywords: ChIP-seq; Drosophila; Sine oculis; development; eye