title: Pathogenesis of a Chinese strain of bovine adenovirus type 3 infection in albino guinea pigs.
abstract: Bovine adenovirus type 3 (BAV-3) is considered one of the most important respiratory tract agents of cattle and is widespread among cattle around the world. A BAV-3 strain was isolated from a bovine nasal swab for the first time in China in 2009 and named HLJ0955. Subsequently, BAV-3 has frequently been isolated from calves with respiratory diseases in China. To date, only limited study on the pathogenesis of BAV-3 infection in cotton rats has been conducted, and the pathogenesis of BAV-3 infection in guinea pigs has not been reported. Therefore, sixteen albino guinea pigs were inoculated intranasally with HLJ0955. All of the infected guinea pigs had apparently elevated rectal temperatures (39.2 °C-39.9 °C) at 2-7 days post-inoculation (PI). Consolidation and petechial hemorrhage were also observed in guinea pigs experimentally infected with HLJ0955. Viral replication was detectable by virus isolation and titration and by immunohistochemistry in the lungs of guinea pigs as early as 24 h PI. Viral DNA was detectable in the lungs of infected guinea pigs during 11 days of observation by real-time PCR. Virus-neutralizing antibodies against BAV-3 were detectable from 11 days PI and reached a peak titer at 15 days PI. Histopathological changes mainly occurred in the lungs of infected guinea pigs and were characterized by thickening of alveolar septa, mononuclear cell infiltration, hemorrhage and alveolar epithelial necrosis. These results indicate that HLJ0955 can replicate in the lungs of guinea pigs and cause fever and gross and histological lesions. The guinea pig infection model of BAV-3 would serve as a useful system for monitoring the infection process and pathogenesis of the Chinese BAV-3 strain HLJ0955, as well as immune responses to BAV-3 vaccines.
authors.affiliation: Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Nan Gang District, Harbin, 150001, Heilongjiang, People's Republic of China.
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Shi
authors.forename: Yuan-Mao
authors.initials: YM
authors.lastname: Zhu
authors.forename: Hao
authors.initials: H
authors.lastname: Yan
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
authors.forename: Xue-Zhi
authors.initials: XZ
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Xue
mesh_terms: D000257:Adenoviridae Infections / Q000473:pathology* / Q000821:virology; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D001831:Body Temperature; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D002681:China; D004195:Disease Models, Animal; D006168:Guinea Pigs; D006470:Hemorrhage; D006651:Histocytochemistry; D007150:Immunohistochemistry; D008168:Lung / Q000473:pathology / Q000821:virology; D017921:Mastadenovirus / Q000254:growth & development* / Q000302:isolation & purification; D009297:Nasal Mucosa / Q000821:virology; D011693:Purpura; D012141:Respiratory Tract Infections / Q000662:veterinary* / Q000821:virology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral