title: Xylan-mediated aggregation of Lactobacillus brevis and its relationship with the surface properties and mucin-mediated aggregation of the bacteria.
abstract: Some Lactobacillus brevis strains were found to aggregate upon the addition of xylan after screening for lactic acid bacteria that interact with plant materials. The S-layer proteins of cell surface varied among the strains. The strains that displayed xylan-mediated aggregation retained its ability even after the removal of S-layer proteins. L. brevis had negative zeta potentials. A correlation between the strength of aggregation and zeta potential was not observed. However, partial removal of S-layer proteins resulted in decreases in the electric potential and aggregation ability of some strains. Therefore, xylan-mediated aggregation of L. brevis was considered to be caused by an electrostatic effect between the cells and xylan. L. brevis also aggregated in the presence of mucin, and the strengths of aggregation among the strains were similar to that induced by xylan. Thus, xylan- and mucin-mediated L. brevis aggregation was supposed to be caused by a similar mechanism.
authors.affiliation: a National Food Research Institute, National Agriculture and Food Research Organization (NARO) , Tsukuba , Japan.
authors.forename: Katsuichi
authors.initials: K
authors.lastname: Saito
authors.forename: Toshihide
authors.initials: T
authors.lastname: Nakamura
authors.forename: Isao
authors.initials: I
authors.lastname: Kobayashi
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ohnishi-Kameyama
authors.forename: Hitomi
authors.initials: H
authors.lastname: Ichinose
authors.forename: Keitarou
authors.initials: K
authors.lastname: Kimura
authors.forename: Kazumi
authors.initials: K
authors.lastname: Funane
mesh_terms: D003470:Culture Media / Q000737:chemistry; D052196:Levilactobacillus brevis / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development; D018021:Lithium Chloride / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000302:isolation & purification; D009077:Mucins / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055672:Static Electricity; D013499:Surface Properties; D014990:Xylans / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D008562:Membrane Glycoproteins; D009077:Mucins; C055764:S-layer proteins; D014990:Xylans; D018021:Lithium Chloride
keywords: Lactobacillus brevis; aggregation; mucin; surface layer protein; xylan