title: Influence of genetic factors on the ephedrine alkaloid composition ratio of Ephedra plants.
abstract: We investigated the ephedrine alkaloid [(-)-ephedrine and (+)-pseudoephedrine] composition ratio of a crude Chinese herbal drug described in the Japanese Pharmacopoeia 'Ephedra herb (Chinese name: Mahuang)'. There were marked changes in the alkaloid composition ratio of wild plants in areas where both male and female clusters coexisted. However, in genetically homogeneous areas with the growth of male or female clusters alone, all of the coefficients of the regression lines were positive, but each gradient varied. This suggests that the alkaloid composition ratio has a clear tendency in each individual. Based on this, we cultivated individuals for vegetative propagation, and evaluated the alkaloid content ratio. Those propagated by separating the roots showed a specific tendency regardless of the cultivation area (Wakayama, Tanegashima). Those propagated by separating the herbaceous stem showed a specific tendency regardless of the soil or harvest time. In addition, we surveyed the (-)-ephedrine content ratio of 3- to 6-year-old strains. There was a high positive correlation coefficient between the previous and subsequent years. These findings suggest that the ephedrine alkaloid composition ratio of Ephedra herb depends on genetic factors, but not on environmental factors or the growth period.
authors.affiliation: Kracie Pharma Ltd., 3-1, Kanebo-machi, Takaoka, Toyama, 933-0856, Japan, matsumoto_masashi@phm.kracie.co.jp.
authors.forename: Masashi
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Manabu
authors.initials: M
authors.lastname: Hirayama
authors.forename: Norihiro
authors.initials: N
authors.lastname: Ohtomi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ohno
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Nomura
authors.forename: Osamu
authors.initials: O
authors.lastname: Iida
authors.forename: Koji
authors.initials: K
authors.lastname: Sugimura
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kawahara
authors.forename: Takashi
authors.initials: T
authors.lastname: Tsuchida
authors.forename: Masayuki
authors.initials: M
authors.lastname: Mikage
mesh_terms: D000470:Alkaloids / Q000032:analysis; D029790:Ephedra sinica / Q000737:chemistry* / Q000235:genetics; D004809:Ephedrine / Q000032:analysis*; D054199:Pseudoephedrine / Q000032:analysis*
chemical_list: D000470:Alkaloids; D054199:Pseudoephedrine; D004809:Ephedrine