title: G-protein αq participates in the steroid hormone 20-hydroxyecdysone nongenomic signal transduction.
abstract: The nuclear receptor-mediated genomic pathways of the animal steroid hormones are well known. However, the cell membrane receptor-mediated nongenomic pathways of the animal steroid hormones are little understood. In this study, we report the participation of a G-protein alpha q (Gαq)(1) subunit in the 20E nongenomic pathway in the cell membrane and regulating gene expression during molting and metamorphosis in a lepidopteran insect, Helicoverpa armigera. 20E-induced phosphorylation of Gαq was detected using two-dimensional electrophoresis techniques. Knockdown of Gαq by injecting double-stranded RNA suppressed the development of larvae, delayed metamorphosis, and inhibited 20E-induced gene expression. Gαq was distributed throughout the cell, and migrated toward the plasma membrane upon 20E induction. Gαq was necessary in the 20E-induced intracellular Ca(2+) release and extracellular Ca(2+) influx. The protein kinase C (PKC) inhibitor could repress 20E-induced phosphorylation of cyclin-dependent kinase 10 (CDK10) and transcription factor ultraspiracle (USP1). PKC inhibitor could repress the Gαq phosphorylation and membrane trafficking. These results suggest that Gαq participates in 20E signaling in the cell membrane at the pre-genomic stage by modulating the increase of the intracellular Ca(2+) and phosphorylation of CDK10 and USP1 in 20E transcription complex to regulate gene transcription.
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan 250100, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ren
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan 250100, China.
authors.forename: Xiang-Ru
authors.initials: XR
authors.lastname: Li
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan 250100, China.
authors.forename: Peng-Cheng
authors.initials: PC
authors.lastname: Liu
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan 250100, China.
authors.forename: Mei-Juan
authors.initials: MJ
authors.lastname: Cai
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan 250100, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Liu
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan 250100, China.
authors.forename: Jin-Xing
authors.initials: JX
authors.lastname: Wang
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan 250100, China. Electronic address: xfzhao@sdu.edu.cn.
authors.forename: Xiao-Fan
authors.initials: XF
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D004441:Ecdysterone / Q000494:pharmacology*; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000235:genetics / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D007915:Lepidoptera; D008675:Metamorphosis, Biological / Q000502:physiology; D010766:Phosphorylation / Q000187:drug effects; D011493:Protein Kinase C / Q000378:metabolism; D034622:RNA Interference; D015398:Signal Transduction / Q000187:drug effects; D064570:Ubiquitin-Specific Proteases / Q000378:metabolism
chemical_list: D019476:Insect Proteins; D004441:Ecdysterone; D011493:Protein Kinase C; D018844:Cyclin-Dependent Kinases; D064570:Ubiquitin-Specific Proteases; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D002118:Calcium
keywords: Ca(2+) influx; G-protein alpha q; Nongenomic signaling; Protein phosphorylation; Steroid hormone