title: A protective role of murine langerin⁺ cells in immune responses to cutaneous vaccination with microneedle patches.
abstract: Cutaneous vaccination with microneedle patches offers several advantages over more frequently used approaches for vaccine delivery, including improved protective immunity. However, the involvement of specific APC subsets and their contribution to the induction of immunity following cutaneous vaccine delivery is not well understood. A better understanding of the functions of individual APC subsets in the skin will allow us to target specific skin cell populations in order to further enhance vaccine efficacy. Here we use a Langerin-EGFP-DTR knock-in mouse model to determine the contribution of langerin(+) subsets of skin APCs in the induction of adaptive immune responses following cutaneous microneedle delivery of influenza vaccine. Depletion of langerin(+) cells prior to vaccination resulted in substantial impairment of both Th1 and Th2 responses, and decreased post-challenge survival rates, in mice vaccinated cutaneously but not in those vaccinated via the intramuscular route or in non-depleted control mice. Our results indicate that langerin(+) cells contribute significantly to the induction of protective immune responses following cutaneous vaccination with a subunit influenza vaccine.
authors.affiliation: Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, Atlanta, Georgia, 30322.
authors.forename: Joanna A
authors.initials: JA
authors.lastname: Pulit-Penaloza
authors.affiliation: Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, Atlanta, Georgia, 30322.
authors.forename: E Stein
authors.initials: ES
authors.lastname: Esser
authors.affiliation: Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, Atlanta, Georgia, 30322.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Vassilieva
authors.affiliation: School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia, 30332.
authors.forename: Jeong Woo
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, Atlanta, Georgia, 30322.
authors.forename: Misha T
authors.initials: MT
authors.lastname: Taherbhai
authors.affiliation: 1] Department of Veterans Affairs, Atlanta VA Medical Center, Decatur, Georgia 30033 [2] Department of Dermatology, Emory University, School of Medicine, Atlanta, Georgia 30322.
authors.forename: Brian P
authors.initials: BP
authors.lastname: Pollack
authors.affiliation: School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia, 30332.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Prausnitz
authors.affiliation: Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, Atlanta, Georgia, 30322.
authors.forename: Richard W
authors.initials: RW
authors.lastname: Compans
authors.affiliation: Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, Atlanta, Georgia, 30322.
authors.forename: Ioanna
authors.initials: I
authors.lastname: Skountzou
mesh_terms: D056704:Adaptive Immunity / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D055879:Gene Knock-In Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D007074:Immunoglobulin G / Q000097:blood; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D007271:Injections, Intradermal; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism*; D061985:Madin Darby Canine Kidney Cells; D037241:Mannose-Binding Lectins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009976:Orthomyxoviridae Infections / Q000517:prevention & control*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014611:Vaccination / Q000379:methods; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000954:Antigens, Surface; C498497:Cd207 protein, mouse; D007074:Immunoglobulin G; D007252:Influenza Vaccines; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins; D022223:Vaccines, Subunit; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins