title: Yeast synthetic biology for the production of recombinant therapeutic proteins.
abstract: The production of recombinant therapeutic proteins is one of the fast-growing areas of molecular medicine and currently plays an important role in treatment of several diseases. Yeasts are unicellular eukaryotic microbial host cells that offer unique advantages in producing biopharmaceutical proteins. Yeasts are capable of robust growth on simple media, readily accommodate genetic modifications, and incorporate typical eukaryotic post-translational modifications. Saccharomyces cerevisiae is a traditional baker's yeast that has been used as a major host for the production of biopharmaceuticals; however, several nonconventional yeast species including Hansenula polymorpha, Pichia pastoris, and Yarrowia lipolytica have gained increasing attention as alternative hosts for the industrial production of recombinant proteins. In this review, we address the established and emerging genetic tools and host strains suitable for recombinant protein production in various yeast expression systems, particularly focusing on current efforts toward synthetic biology approaches in developing yeast cell factories for the production of therapeutic recombinant proteins.
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul, Korea.
authors.forename: Hyunah
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul, Korea.
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Yoo
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul, Korea hyunkang@cau.ac.kr.
authors.forename: Hyun Ah
authors.initials: HA
authors.lastname: Kang
mesh_terms: D001702:Biopharmaceutics; D015870:Gene Expression; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism* / Q000627:therapeutic use; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D058615:Synthetic Biology / Q000379:methods*; D025062:Yarrowia / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins
keywords: biopharmaceuticals; glycoengineering; recombinant protein; secretory pathway engineering; yeast cell factory