title: Molecular detection of Mayaro virus during a dengue outbreak in the state of Mato Grosso, Central-West Brazil.
abstract: Mayaro virus (MAYV) is frequently reported in Pan-Amazonia. The aim of this study was to investigate the circulation of alphaviruses during a dengue outbreak in the state of Mato Grosso, Brazil. Serum samples from dengue-suspected patients were subjected to multiplex semi-nested reverse transcriptase polymerase chain reaction for 11 flaviviruses and five alphaviruses, to nucleotide sequencing and to viral isolation. MAYV was detected in 15 (2.5%) of 604 patients. Twelve were co-infected with dengue virus 4, which was isolated from 10 patients. The molecular detection of MAYV in dengue-suspected patients suggests that other arboviruses may be silently circulating during dengue outbreaks in Brazil.
authors.affiliation: Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil.
authors.forename: Nayara
authors.initials: N
authors.lastname: Zuchi
authors.affiliation: Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil.
authors.forename: Letícia Borges da Silva
authors.initials: LB
authors.lastname: Heinen
authors.affiliation: MT Laboratório, Secretaria Estadual de Saúde, Cuiabá, MT, Brasil.
authors.forename: Marcelo Adriano Mendes dos
authors.initials: MA
authors.lastname: Santos
authors.affiliation: Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil.
authors.forename: Fernanda Carla
authors.initials: FC
authors.lastname: Pereira
authors.affiliation: Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil.
authors.forename: Renata Dezengrini
authors.initials: RD
authors.lastname: Slhessarenko
mesh_terms: D000293:Adolescent; D000328:Adult; D000524:Alphavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018354:Alphavirus Infections / Q000453:epidemiology*; D058345:Asymptomatic Infections / Q000453:epidemiology*; D001483:Base Sequence / Q000235:genetics; D001938:Brazil / Q000453:epidemiology; D002648:Child; D060085:Coinfection / Q000453:epidemiology / Q000821:virology; D003715:Dengue / Q000097:blood / Q000453:epidemiology* / Q000821:virology; D003716:Dengue Virus / Q000302:isolation & purification*; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods; D060885:Multiplex Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult