title: Differential response of MCF7, MDA-MB-231, and MCF 10A cells to hyperthermia, silver nanoparticles and silver nanoparticle-induced photothermal therapy.
abstract: OBJECTIVE
  Silver nanoparticles (Ag NP) can generate heat upon exposure to infrared light. The in vitro response of breast cell lines to Ag NP, both with and without nanoparticle-induced heating was evaluated.
  
  
  METHODS
  Ag NP heat generation, intracellular silver concentration, and cell viability of MDA-MB-231, MCF7, and MCF 10A breast cells with Ag NP alone, or after exposure to 0.79 or 2.94 W/cm2 of 800 nm light were evaluated.
  
  
  RESULTS
  The concentration of Ag NP to induce sufficient heat for cell death, upon exposure to 800 nm light, was 5-250 μg/mL. Clonogenics assay indicates a cytotoxic response of MCF7 (45% decrease) and MDA-MB-231 (80% decrease) cells to 10 µg/mL, whereas MCF 10A had a 25% increase. Without Ag NP, MDA-MB-231 cells were more susceptible to hyperthermia, compared to MCF7 and MCF 10A cells. Clonogenics assay of Ag NP-induced photothermal ablation demonstrated that MCF 10A cells have the highest survival fraction. MCF7 cells had more silver in the cytoplasm, MDA-MB-231 cells had more in the nuclei, and MCF 10A cells had equivalent concentrations in the cytoplasm and nuclei.
  
  
  CONCLUSIONS
  Ag NP are effective photothermal agents. A secondary benefit is the differential response of breast cancer cells to Ag NP-induced hyperthermia, due to increased intracellular silver content, compared to non-tumorigenic breast epithelial cells.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Wake Forest University Health Sciences , Winston-Salem , North Carolina .
authors.forename: Edreca A
authors.initials: EA
authors.lastname: Thompson
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Graham
authors.forename: Christopher M
authors.initials: CM
authors.lastname: MacNeill
authors.forename: Michelle
authors.initials: M
authors.lastname: Young
authors.forename: George
authors.initials: G
authors.lastname: Donati
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Wailes
authors.forename: Bradley T
authors.initials: BT
authors.lastname: Jones
authors.forename: Nicole H
authors.initials: NH
authors.lastname: Levi-Polyachenko
mesh_terms: D001943:Breast Neoplasms / Q000628:therapy; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D006979:Hyperthermia, Induced; D007259:Infrared Rays; D053768:Metal Nanoparticles; D010789:Phototherapy; D012834:Silver / Q000494:pharmacology*
chemical_list: D012834:Silver
keywords: Breast; cancer; photothermal therapy; silver nanoparticles