title: Identification of molecular target of diallyl trisulfide in leukemic cells.
abstract: To identify the molecular target of diallyl trisulfide (DATS) in human leukemic cell line U937, we examined modification of thiol group(s) of cellular proteins by the redox 2D PAGE. A unique protein spot appeared by DATS treatment was identified to be heat shock protein 27 (HSP27). Hsp27 is suggested to be one of the molecular target of DATS in U937.
authors.affiliation: a Department of Chemistry and Life Science , College of Bioresource Sciences, Nihon University , Fujisawa , Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Suda
authors.forename: Kazunari
authors.initials: K
authors.lastname: Watanabe
authors.forename: Yuki
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Watanabe
authors.forename: Rie
authors.initials: R
authors.lastname: Tanaka
authors.forename: Jun
authors.initials: J
authors.lastname: Ogihara
authors.forename: Toyohiko
authors.initials: T
authors.lastname: Ariga
authors.forename: Tomomi
authors.initials: T
authors.lastname: Hosono-Fukao
authors.forename: Takashi
authors.initials: T
authors.lastname: Hosono
authors.forename: Taiichiro
authors.initials: T
authors.lastname: Seki
mesh_terms: D000498:Allyl Compounds / Q000494:pharmacology* / Q000627:therapeutic use; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D007938:Leukemia / Q000188:drug therapy / Q000473:pathology*; D058990:Molecular Targeted Therapy; D013440:Sulfides / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000498:Allyl Compounds; D000970:Antineoplastic Agents; D013440:Sulfides; C042577:diallyl trisulfide
keywords: U937; diagonal gel electrophoresis; diallyl trisulfide; garlic; heat shock protein 27 (HSP27)