title: Architecture of the Saccharomyces cerevisiae RNA polymerase I Core Factor complex.
abstract: Core Factor (CF) is a conserved RNA polymerase (Pol) I general transcription factor comprising Rrn6, Rrn11 and the TFIIB-related subunit Rrn7. CF binds TATA-binding protein (TBP), Pol I and the regulatory factors Rrn3 and upstream activation factor. We used chemical cross-linking-MS to determine the molecular architecture of CF and its interactions with TBP. The CF subunits assemble through an interconnected network of interactions between five structural domains that are conserved in orthologous subunits of the human Pol I factor SL1. We validated the cross-linking-derived model through a series of genetic and biochemical assays. Our combined results show the architecture of CF and the functions of the CF subunits in assembly of the complex. We extend these findings to model how CF assembles into the Pol I preinitiation complex, providing new insight into the roles of CF, TBP and Rrn3.
authors.affiliation: 1] Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA. [2].
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Knutson
authors.affiliation: Institute for Systems Biology, Seattle, Washington, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Luo
authors.affiliation: Institute for Systems Biology, Seattle, Washington, USA.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Ranish
authors.affiliation: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
authors.forename: Steven
authors.initials: S
authors.lastname: Hahn
mesh_terms: D008958:Models, Molecular; D035463:Pol1 Transcription Initiation Complex Proteins / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D012318:RNA Polymerase I / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D035463:Pol1 Transcription Initiation Complex Proteins; D021122:Protein Subunits; C542286:RRN11 protein, S cerevisiae; C090311:RRN6 protein, S cerevisiae; C090315:RRN7 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D012318:RNA Polymerase I