title: Association of CVD candidate gene polymorphisms with ischemic stroke and cerebral hemorrhage in Chinese individuals.
abstract: BACKGROUND
  Contribution of cardiovascular disease related genetic risk factors for stroke are not clearly defined. We performed a genetic association study to assess the association of 56 previously characterized gene variants in 34 candidate genes from cardiovascular disease related biological pathways with ischemic stroke and cerebral hemorrhage in a Chinese population.
  
  
  METHODS
  There were 1280 stroke patients (1101 with ischemic stroke and 179 with cerebral hemorrhage) and 1380 controls in the study. The genotypes for 56 polymorphisms of 34 candidate genes were determined by the immobilized probe approach and the associations of gene polymorphisms with ischemic stroke and cerebral hemorrhage were performed by logistic regression under an allelic model.
  
  
  RESULTS
  After adjusting for age, sex, BMI and hypertension status by logistic regression analysis, we found that NPPA rs5063 was significantly associated with both ischemic stroke (odds ratio [OR] 0.69; 95% confidence interval [CI], 0.52 to 0.90; P = 0.006) and cerebral hemorrhage(OR = 0.39; 95%CI, 0.19 to 0.78; P = 0.007). In addition, MTHFR rs1801133 also was associated with cerebral hemorrhage (OR = 1.48; 95%CI, 1.16 to 1.89; P = 0.001) but not with ischemic stroke (OR = 1.08; 95%CI, 0.96 to 1.22; P = 0.210). After false discovery rate (FDR) correction, the association of NPPA rs5063 and MTHFR rs1801133 with cerebral hemorrhage remained significant.
  
  
  CONCLUSIONS
  The NPPA rs5063 is associated with reduced risk for cerebral hemorrhage and MTHFR rs1801133 is associated with increased risk of cerebral hemorrhage in a Chinese population.
authors.affiliation: Department of Epidemiology, Public Health School, Harbin Medical University, Heilongjiang, China; Department of Epidemiology, Public Health School, Harbin Medical University, Heilongjiang, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Ou
authors.affiliation: The Laboratory of Human Genetics, Beijing Hypertension League Institute, Beijing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: The Laboratory of Human Genetics, Beijing Hypertension League Institute, Beijing, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Epidemiology, Public Health School, Harbin Medical University, Heilongjiang, China.
authors.forename: Jiana
authors.initials: J
authors.lastname: Li
authors.affiliation: First Affiliated Hospital, Medical College of Shantou University, Shantou, Guangdong, China.
authors.forename: Lingbin
authors.initials: L
authors.lastname: He
authors.affiliation: First Affiliated Hospital, Medical College of Shantou University, Shantou, Guangdong, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: First Affiliated Hospital, Medical College of Shantou University, Shantou, Guangdong, China.
authors.forename: Xuerui
authors.initials: X
authors.lastname: Tan
authors.affiliation: The Laboratory of Human Genetics, Beijing Hypertension League Institute, Beijing, China.
authors.forename: Lisheng
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Epidemiology, Public Health School, Harbin Medical University, Heilongjiang, China.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Zhao
authors.affiliation: First Affiliated Hospital, Medical College of Shantou University, Shantou, Guangdong, China; The Laboratory of Human Genetics, Beijing Hypertension League Institute, Beijing, China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Wang
mesh_terms: D000368:Aged; D002318:Cardiovascular Diseases / Q000150:complications* / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D002543:Cerebral Hemorrhage / Q000453:epidemiology* / Q000209:etiology*; D002681:China / Q000453:epidemiology; D005260:Female; D056726:Genetic Association Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D020521:Stroke / Q000453:epidemiology* / Q000209:etiology*