title: Activated group 3 innate lymphoid cells promote T-cell-mediated immune responses.
abstract: Group 3 innate lymphoid cells (ILC3s) have emerged as important cellular players in tissue repair and innate immunity. Whether these cells meaningfully regulate adaptive immune responses upon activation has yet to be explored. Here we show that upon IL-1β stimulation, peripheral ILC3s become activated, secrete cytokines, up-regulate surface MHC class II molecules, and express costimulatory molecules. ILC3s can take up latex beads, process protein antigen, and consequently prime CD4(+) T-cell responses in vitro. The cognate interaction of ILC3s and CD4(+) T cells leads to T-cell proliferation both in vitro and in vivo, whereas its disruption impairs specific T-cell and T-dependent B-cell responses in vivo. In addition, the ILC3-CD4(+) T-cell interaction is bidirectional and leads to the activation of ILC3s. Taken together, our data reveal a novel activation-dependent function of peripheral ILC3s in eliciting cognate CD4(+) T-cell immune responses.
authors.affiliation: Department of Biomedicine, University of Basel, 4058 Basel, Switzerland; University Children's Hospital of Basel, 4056 Basel, Switzerland;
authors.forename: Nicole
authors.initials: N
authors.lastname: von Burg
authors.affiliation: Department of Biomedicine, University of Basel, 4058 Basel, Switzerland; Australian Cancer Research Foundation Chemical Biology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 3010, Australia;
authors.forename: Stéphane
authors.initials: S
authors.lastname: Chappaz
authors.affiliation: Department of Biomedicine, University of Basel, 4058 Basel, Switzerland; University Children's Hospital of Basel, 4056 Basel, Switzerland;
authors.forename: Anne
authors.initials: A
authors.lastname: Baerenwaldt
authors.affiliation: Department of Biomedicine, University of Basel, 4058 Basel, Switzerland; University Children's Hospital of Basel, 4056 Basel, Switzerland;
authors.forename: Edit
authors.initials: E
authors.lastname: Horvath
authors.affiliation: Biozentrum, University of Basel, 4056 Basel, Switzerland; and.
authors.forename: Somdeb
authors.initials: S
authors.lastname: Bose Dasgupta
authors.affiliation: Department of Biochemistry and.
authors.forename: Devika
authors.initials: D
authors.lastname: Ashok
authors.affiliation: Biozentrum, University of Basel, 4056 Basel, Switzerland; and.
authors.forename: Jean
authors.initials: J
authors.lastname: Pieters
authors.affiliation: Department of Biochemistry and World Health Organization Immunology Research and Training Centre, University of Lausanne, 1066 Epalinges, Switzerland.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Tacchini-Cottier
authors.affiliation: Department of Biomedicine, University of Basel, 4058 Basel, Switzerland;
authors.forename: Antonius
authors.initials: A
authors.lastname: Rolink
authors.affiliation: Department of Biochemistry and.
authors.forename: Hans
authors.initials: H
authors.lastname: Acha-Orbea
authors.affiliation: Department of Biomedicine, University of Basel, 4058 Basel, Switzerland; University Children's Hospital of Basel, 4056 Basel, Switzerland; Daniela.Finke@unibas.ch.
authors.forename: Daniela
authors.initials: D
authors.lastname: Finke
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007111:Immunity, Cellular / Q000276:immunology; D053583:Interleukin-1beta / Q000276:immunology* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D015398:Signal Transduction / Q000276:immunology*
chemical_list: D016207:Cytokines; D000949:Histocompatibility Antigens Class II; D053583:Interleukin-1beta
keywords: T-cell activation; antigen presentation