title: Collagen mimetic peptide engineered M13 bacteriophage for collagen targeting and imaging in cancer.
abstract: Collagens are over-expressed in various human cancers and subsequently degraded and denatured by proteolytic enzymes, thus making them a target for diagnostics and therapeutics. Genetically engineered bacteriophage (phage) is a promising candidate for the development of imaging or therapeutic materials for cancer collagen targeting due to its promising structural features. We genetically engineered M13 phages with two functional peptides, collagen mimetic peptide and streptavidin binding peptide, on their minor and major coat proteins, respectively. The resulting engineered phage functions as a therapeutic or imaging material to target degraded and denatured collagens in cancerous tissues. We demonstrated that the engineered phages are able to target and label abnormal collagens expressed on A549 human lung adenocarcinoma cells after the conjugation with streptavidin-linked fluorescent agents. Our engineered collagen binding phage could be a useful platform for abnormal collagen imaging and drug delivery in various collagen-related diseases.
authors.affiliation: Department of Bioengineering, University of California, Berkeley, CA 94720, USA; Physical Bioscience Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
authors.forename: Hyo-Eon
authors.initials: HE
authors.lastname: Jin
authors.affiliation: Department of Bioengineering, University of California, Berkeley, CA 94720, USA; Physical Bioscience Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Farr
authors.affiliation: Department of Bioengineering, University of California, Berkeley, CA 94720, USA; Physical Bioscience Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address: leesw@berkeley.edu.
authors.forename: Seung-Wuk
authors.initials: SW
authors.lastname: Lee
mesh_terms: D000230:Adenocarcinoma; D000077192:Adenocarcinoma of Lung; D017104:Bacteriophage M13 / Q000235:genetics*; D032701:Biomimetics; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D045744:Cell Line, Tumor; D003094:Collagen / Q000737:chemistry / Q000235:genetics*; D003952:Diagnostic Imaging / Q000379:methods*; D016503:Drug Delivery Systems; D005456:Fluorescent Dyes; D006801:Humans; D008175:Lung Neoplasms; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism; D009369:Neoplasms / Q000628:therapy; D010455:Peptides / Q000737:chemistry / Q000235:genetics; D011485:Protein Binding; D019809:Streptavidin / Q000737:chemistry; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D005456:Fluorescent Dyes; D010455:Peptides; D053773:Transforming Growth Factor beta1; D003094:Collagen; D019809:Streptavidin; D020782:Matrix Metalloproteinases
keywords: Bacteriophage; Cancer; Collagen; Imaging; Targeting