title: Light sheet fluorescence microscopy (LSFM): past, present and future.
abstract: Light sheet fluorescence microscopy (LSFM) has emerged as an important imaging modality to follow biology in live 3D samples over time with reduced phototoxicity and photobleaching. In particular, LSFM has been instrumental in revealing the detail of early embryonic development of Zebrafish, Drosophila, and C. elegans. Open access projects, DIY-SPIM, OpenSPIM, and OpenSPIN, now allow LSFM to be set-up easily and at low cost. The aim of this paper is to facilitate the set-up and use of LSFM by reviewing and comparing open access projects, image processing tools and future challenges.
authors.affiliation: Institute of Medical Biology, 8A Biomedical Grove, Immunos 5.37, Singapore 138648, Singapore. sohail.ahmed@imb.a-star.edu.sg.
authors.forename: John
authors.initials: J
authors.lastname: Lim
authors.forename: Hwee Kuan
authors.initials: HK
authors.lastname: Lee
authors.forename: Weimiao
authors.initials: W
authors.lastname: Yu
authors.forename: Sohail
authors.initials: S
authors.lastname: Ahmed
mesh_terms: D000818:Animals; D058948:Cell Tracking; D047108:Embryonic Development; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010363:Pattern Recognition, Automated