title: Biointerface multiparametric study of intraocular lens acrylic materials.
abstract: OBJECTIVE
  To compare hydrophilic and hydrophobic acrylic materials designed for intraocular lenses in a multiparametric investigation in a liquid environment to highlight their properties in terms of adhesion forces, lens epithelial cell (LEC) adhesion, and tissue response as indicators of the risk for posterior capsule opacification (PCO) development.
  
  
  METHODS
  University of Liège, Liège, Belgium.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  The hydrophobicity and surface adhesion force were assessed using contact-angle and atomic force microscopy measurements. The bioadhesiveness of the disks and the tissue response were determined by in vitro experiments using bovine serum albumin and porcine LECs and by in vivo rabbit subcutaneous implantation, respectively.
  
  
  RESULTS
  Increasing surface hydrophobicity led to a greater surface-adhesion force and greater LEC adhesion. After 1 month, the rabbit subcutaneous implants showed a similar thin layer of fibrous capsule surrounding the disks without extensive inflammation. A layer of rounded cells in contact with disks was detected on the hydrophobic samples only.
  
  
  CONCLUSIONS
  Hydrophobic acrylic disks that have been associated with a reduced risk for PCO in clinical studies showed increased tackiness.
  
  
  BACKGROUND
  Proprietary or commercial disclosures are listed after the references.
authors.affiliation: From the Mammalian Cell Culture Laboratory (Bertrand, Huang, Rosière, De Pauw-Gillet) and Mass Spectrometry Laboratory (Bertrand, Rosière, De Pauw), Groupe Interdisciplinaire de Génoprotéomique Appliquée-Research University of Liège, the NanoChemistry and Molecular Systems Laboratory (Svaldo Lanero, Duwez), the Veterinary Ophthalmology (Grauwels), the Center of Education and Research on Macromolecules (Jérôme), University of Liège, and the Research and Development Department (Bozukova, Pagnoulle), PhysIOL s.a., Liège, Belgium.
authors.forename: Virginie
authors.initials: V
authors.lastname: Bertrand
authors.affiliation: From the Mammalian Cell Culture Laboratory (Bertrand, Huang, Rosière, De Pauw-Gillet) and Mass Spectrometry Laboratory (Bertrand, Rosière, De Pauw), Groupe Interdisciplinaire de Génoprotéomique Appliquée-Research University of Liège, the NanoChemistry and Molecular Systems Laboratory (Svaldo Lanero, Duwez), the Veterinary Ophthalmology (Grauwels), the Center of Education and Research on Macromolecules (Jérôme), University of Liège, and the Research and Development Department (Bozukova, Pagnoulle), PhysIOL s.a., Liège, Belgium.
authors.forename: Dimitriya
authors.initials: D
authors.lastname: Bozukova
authors.affiliation: From the Mammalian Cell Culture Laboratory (Bertrand, Huang, Rosière, De Pauw-Gillet) and Mass Spectrometry Laboratory (Bertrand, Rosière, De Pauw), Groupe Interdisciplinaire de Génoprotéomique Appliquée-Research University of Liège, the NanoChemistry and Molecular Systems Laboratory (Svaldo Lanero, Duwez), the Veterinary Ophthalmology (Grauwels), the Center of Education and Research on Macromolecules (Jérôme), University of Liège, and the Research and Development Department (Bozukova, Pagnoulle), PhysIOL s.a., Liège, Belgium.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Svaldo Lanero
authors.affiliation: From the Mammalian Cell Culture Laboratory (Bertrand, Huang, Rosière, De Pauw-Gillet) and Mass Spectrometry Laboratory (Bertrand, Rosière, De Pauw), Groupe Interdisciplinaire de Génoprotéomique Appliquée-Research University of Liège, the NanoChemistry and Molecular Systems Laboratory (Svaldo Lanero, Duwez), the Veterinary Ophthalmology (Grauwels), the Center of Education and Research on Macromolecules (Jérôme), University of Liège, and the Research and Development Department (Bozukova, Pagnoulle), PhysIOL s.a., Liège, Belgium.
authors.forename: Yi-Shiang
authors.initials: YS
authors.lastname: Huang
authors.affiliation: From the Mammalian Cell Culture Laboratory (Bertrand, Huang, Rosière, De Pauw-Gillet) and Mass Spectrometry Laboratory (Bertrand, Rosière, De Pauw), Groupe Interdisciplinaire de Génoprotéomique Appliquée-Research University of Liège, the NanoChemistry and Molecular Systems Laboratory (Svaldo Lanero, Duwez), the Veterinary Ophthalmology (Grauwels), the Center of Education and Research on Macromolecules (Jérôme), University of Liège, and the Research and Development Department (Bozukova, Pagnoulle), PhysIOL s.a., Liège, Belgium.
authors.forename: Daureen
authors.initials: D
authors.lastname: Schol
authors.affiliation: From the Mammalian Cell Culture Laboratory (Bertrand, Huang, Rosière, De Pauw-Gillet) and Mass Spectrometry Laboratory (Bertrand, Rosière, De Pauw), Groupe Interdisciplinaire de Génoprotéomique Appliquée-Research University of Liège, the NanoChemistry and Molecular Systems Laboratory (Svaldo Lanero, Duwez), the Veterinary Ophthalmology (Grauwels), the Center of Education and Research on Macromolecules (Jérôme), University of Liège, and the Research and Development Department (Bozukova, Pagnoulle), PhysIOL s.a., Liège, Belgium.
authors.forename: Nancy
authors.initials: N
authors.lastname: Rosière
authors.affiliation: From the Mammalian Cell Culture Laboratory (Bertrand, Huang, Rosière, De Pauw-Gillet) and Mass Spectrometry Laboratory (Bertrand, Rosière, De Pauw), Groupe Interdisciplinaire de Génoprotéomique Appliquée-Research University of Liège, the NanoChemistry and Molecular Systems Laboratory (Svaldo Lanero, Duwez), the Veterinary Ophthalmology (Grauwels), the Center of Education and Research on Macromolecules (Jérôme), University of Liège, and the Research and Development Department (Bozukova, Pagnoulle), PhysIOL s.a., Liège, Belgium.
authors.forename: Magda
authors.initials: M
authors.lastname: Grauwels
authors.affiliation: From the Mammalian Cell Culture Laboratory (Bertrand, Huang, Rosière, De Pauw-Gillet) and Mass Spectrometry Laboratory (Bertrand, Rosière, De Pauw), Groupe Interdisciplinaire de Génoprotéomique Appliquée-Research University of Liège, the NanoChemistry and Molecular Systems Laboratory (Svaldo Lanero, Duwez), the Veterinary Ophthalmology (Grauwels), the Center of Education and Research on Macromolecules (Jérôme), University of Liège, and the Research and Development Department (Bozukova, Pagnoulle), PhysIOL s.a., Liège, Belgium.
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Duwez
authors.affiliation: From the Mammalian Cell Culture Laboratory (Bertrand, Huang, Rosière, De Pauw-Gillet) and Mass Spectrometry Laboratory (Bertrand, Rosière, De Pauw), Groupe Interdisciplinaire de Génoprotéomique Appliquée-Research University of Liège, the NanoChemistry and Molecular Systems Laboratory (Svaldo Lanero, Duwez), the Veterinary Ophthalmology (Grauwels), the Center of Education and Research on Macromolecules (Jérôme), University of Liège, and the Research and Development Department (Bozukova, Pagnoulle), PhysIOL s.a., Liège, Belgium.
authors.forename: Christine
authors.initials: C
authors.lastname: Jérôme
authors.affiliation: From the Mammalian Cell Culture Laboratory (Bertrand, Huang, Rosière, De Pauw-Gillet) and Mass Spectrometry Laboratory (Bertrand, Rosière, De Pauw), Groupe Interdisciplinaire de Génoprotéomique Appliquée-Research University of Liège, the NanoChemistry and Molecular Systems Laboratory (Svaldo Lanero, Duwez), the Veterinary Ophthalmology (Grauwels), the Center of Education and Research on Macromolecules (Jérôme), University of Liège, and the Research and Development Department (Bozukova, Pagnoulle), PhysIOL s.a., Liège, Belgium.
authors.forename: Christophe
authors.initials: C
authors.lastname: Pagnoulle
authors.affiliation: From the Mammalian Cell Culture Laboratory (Bertrand, Huang, Rosière, De Pauw-Gillet) and Mass Spectrometry Laboratory (Bertrand, Rosière, De Pauw), Groupe Interdisciplinaire de Génoprotéomique Appliquée-Research University of Liège, the NanoChemistry and Molecular Systems Laboratory (Svaldo Lanero, Duwez), the Veterinary Ophthalmology (Grauwels), the Center of Education and Research on Macromolecules (Jérôme), University of Liège, and the Research and Development Department (Bozukova, Pagnoulle), PhysIOL s.a., Liège, Belgium.
authors.forename: Edwin
authors.initials: E
authors.lastname: De Pauw
authors.affiliation: From the Mammalian Cell Culture Laboratory (Bertrand, Huang, Rosière, De Pauw-Gillet) and Mass Spectrometry Laboratory (Bertrand, Rosière, De Pauw), Groupe Interdisciplinaire de Génoprotéomique Appliquée-Research University of Liège, the NanoChemistry and Molecular Systems Laboratory (Svaldo Lanero, Duwez), the Veterinary Ophthalmology (Grauwels), the Center of Education and Research on Macromolecules (Jérôme), University of Liège, and the Research and Development Department (Bozukova, Pagnoulle), PhysIOL s.a., Liège, Belgium. Electronic address: Marie-claire.depauw@ulg.ac.be.
authors.forename: Marie-Claire
authors.initials: MC
authors.lastname: De Pauw-Gillet
mesh_terms: D000180:Acrylic Resins; D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000502:physiology*; D057927:Hydrophobic and Hydrophilic Interactions; D019654:Lens Implantation, Intraocular; D007908:Lens, Crystalline / Q000166:cytology*; D007910:Lenses, Intraocular; D018625:Microscopy, Atomic Force; D011485:Protein Binding; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D040521:Subcutaneous Tissue / Q000601:surgery; D013499:Surface Properties; D013552:Swine; D000267:Tissue Adhesions
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D012710:Serum Albumin, Bovine