title: Lectin-probed western blot analysis.
abstract: Lectin-probed western blot analysis, the so-called lectin blot analysis, is a useful method to yield basic information on the glycan structures of glycoproteins, based on the carbohydrate-binding specificities of lectins. By lectin blot analysis, researchers can directly analyze the glycan structures without releasing the glycans from glycoproteins. Here, the author describes protocols for standard analysis, and applies analysis in combination with glycosidase digestion of blot.
authors.affiliation: Laboratory of Glycobiology, Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, 940-2188, Japan, taksato@vos.nagaokaut.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sato
mesh_terms: D015153:Blotting, Western / Q000379:methods*; D045744:Cell Line, Tumor; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008567:Membranes, Artificial; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: D037102:Lectins; D008567:Membranes, Artificial; D011134:Polysaccharides; D006026:Glycoside Hydrolases