title: Establishment of glycosaminoglycan assays for mucopolysaccharidoses.
abstract: Mucopolysaccharidoses (MPS) are a group of lysosomal storage disorders caused by deficiency of the lysosomal enzymes essential for catabolism of glycosaminoglycans (GAGs). Accumulation of undegraded GAGs results in dysfunction of multiple organs, resulting in distinct clinical manifestations. A range of methods have been developed to measure specific GAGs in various human samples to investigate diagnosis, prognosis, pathogenesis, GAG interaction with other molecules, and monitoring therapeutic efficacy. We established ELISA, liquid chromatography tandem mass spectrometry (LC-MS/MS), and an automated high-throughput mass spectrometry (HT-MS/MS) system (RapidFire) to identify epitopes (ELISA) or disaccharides (MS/MS) derived from different GAGs (dermatan sulfate, heparan sulfate, keratan sulfate, and/or chondroitin sulfate). These methods have a high sensitivity and specificity in GAG analysis, applicable to the analysis of blood, urine, tissues, and cells. ELISA is feasible, sensitive, and reproducible with the standard equipment. HT-MS/MS yields higher throughput than conventional LC-MS/MS-based methods while the HT-MS/MS system does not have a chromatographic step and cannot distinguish GAGs with identical molecular weights, leading to a limitation of measurements for some specific GAGs. Here we review the advantages and disadvantages of these methods for measuring GAG levels in biological specimens. We also describe an unexpected secondary elevation of keratan sulfate in patients with MPS that is an indirect consequence of disruption of catabolism of other GAGs.
authors.affiliation: Nemours/Alfred I duPont Hospital for Children, Wilmington, DE 19803, USA. stomatsu@nemours.org.
authors.forename: Shunji
authors.initials: S
authors.lastname: Tomatsu
authors.affiliation: Nemours/Alfred I duPont Hospital for Children, Wilmington, DE 19803, USA. tsutomu.shimada@nemours.org.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Shimada
authors.affiliation: Nemours/Alfred I duPont Hospital for Children, Wilmington, DE 19803, USA. rmason@nemours.org.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Mason
authors.affiliation: Department of Pediatrics, Saint Louis University, St. Louis, MO 63104, USA. montana@slu.edu.
authors.forename: Adriana M
authors.initials: AM
authors.lastname: Montaño
authors.affiliation: Agilent Technologies, Inc., Wakefield, MA 01880, USA. joan.kelly@agilent.com.
authors.forename: Joan
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authors.lastname: Kelly
authors.affiliation: Agilent Technologies, Inc., Wakefield, MA 01880, USA. william.lamarr@agilent.com.
authors.forename: William A
authors.initials: WA
authors.lastname: LaMarr
authors.affiliation: Nemours/Alfred I duPont Hospital for Children, Wilmington, DE 19803, USA. fkubaski@udel.edu.
authors.forename: Francyne
authors.initials: F
authors.lastname: Kubaski
authors.affiliation: Department of Genetics/UFRGS, Medical Genetics Service/HCPA, Porto Alegre 90035-903, Brazil. rgiugliani@hcpa.ufrgs.br.
authors.forename: Roberto
authors.initials: R
authors.lastname: Giugliani
authors.affiliation: Nemours/Alfred I duPont Hospital for Children, Wilmington, DE 19803, USA. aratrik@udel.edu.
authors.forename: Aratrik
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authors.lastname: Guha
authors.affiliation: Nemours/Alfred I duPont Hospital for Children, Wilmington, DE 19803, USA. Eriko.Yasuda@nemours.org.
authors.forename: Eriko
authors.initials: E
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authors.affiliation: Nemours/Alfred I duPont Hospital for Children, Wilmington, DE 19803, USA. wmackenz@nemours.org.
authors.forename: William
authors.initials: W
authors.lastname: Mackenzie
authors.affiliation: Department of Pediatrics, Shimane University, Shimane 693-8501, Japan. seijiyam@med.shimane-u.ac.jp.
authors.forename: Seiji
authors.initials: S
authors.lastname: Yamaguchi
authors.affiliation: Medical Education Development Center, Gifu University, Gifu 501-1194, Japan. ysuz@gifu-u.ac.jp.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Department of Pediatrics, Gifu University, Gifu 501-1194, Japan. orii.tadao@camel.plala.or.jp.
authors.forename: Tadao
authors.initials: T
authors.lastname: Orii