title: [Relationship of genetic variants and cardiovascular risk factors with interleukin-6 and interleukin-10 secreted by monocytes].
abstract: OBJECTIVE
  To examine the relationship of interleukin (IL)-6 and IL-10 genetic variants and cardiovascular factors [oxygenized low density lipoprotein (ox-LDL), lower physical activity, overweight, etc.] with IL-6 and IL-10 secreted by monocytes.
  
  
  METHODS
  In the study, 40 health persons, aged from 51 to 80 years, without stroke and myocardial infarction, were randomly sampled from a community-based population in Beijing in 2010. Their data on smoking, drinking, blood pressure, fasting glucose, and lipid were collected. The single nucleotide polymorphisms (SNPs) of IL-6 (rs1800796, rs1524107, rs2066992) and IL-10 (rs1800872, rs1554286, rs3021094) were genotyped. The human monocytes were cultivated in RPMI 1640 medium for 24 h; then divided into two equal parts, in which ox-LDL (50 mg/L) and phosphate buffer solution (PBS) were added for another 48 h. Finally, the secretions of IL-6 and IL-10 in the culture supernatants were measured with ELISA.
  
  
  RESULTS
  Paired Wilcoxon tests showed that the IL-6, IL-10, and IL-6/IL-10 were significantly higher in ox-LDL medium than in PBS one (all P < 0.01). The concentrations in PBS/ox-LDL taken as repeated measurements, and adjusted for age and gender, the repeated general linear models showed: IL-10 was significantly lower for those overweight (BMI ≥ 26 kg/m(2)) than for those normal weight (P = 0.007), and IL-6/IL-10 was significantly higher in those overweight (P = 0.003). The IL-6/IL-10 was significantly higher in those with lower physical activity [metabolic equivalent of energy, METS < 166 kJ/(kg.d)] than those with higher physical activities (P = 0.046). IL-6 and IL-10 were significantly higher in alcohol drinkers (P = 0.049 and P = 0.006). IL-6 was significantly higher in those with higher high-density lipoprotein-cholesterol (HDL-c, ≥ 56.4 mg/dL, P = 0.027). There were significant interactions between IL-10 SNPs and ox-LDL on IL-10 (all P < 0.05), but no significant interactions between IL-6 gene SNPs and ox-LDL on IL-6.
  
  
  CONCLUSIONS
  The ox-LDL together with lower physical activity and overweight shifts the balance of pro-inflammatory and anti-inflammatory in the direction of pro-inflammatory. The interaction between IL-10 gene and ox-LDL is intensively correlated with the secretion of the anti-inflammatory cytokine IL-10.
authors.affiliation: Peking University Clinical Research Institute, Beijing 100191, China.
authors.forename: Gao-qiang
authors.initials: GQ
authors.lastname: Xie
authors.affiliation: Sino-German Laboratory for Molecular Medicine, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100037, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yu
authors.affiliation: Sino-German Laboratory for Molecular Medicine, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100037, China.
authors.forename: Jing-zhou
authors.initials: JZ
authors.lastname: Chen
authors.affiliation: Department of Prevention Network, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100037, China.
authors.forename: Lian-cheng
authors.initials: LC
authors.lastname: Zhao
authors.affiliation: Shijingshan Center for Disease Control and Prevention, Beijing 100043, China; 5. Department of Epidemiology and Biostatistics, Peking University School of Public Health, Beijing 100191, China.
authors.forename: Fu-xiu
authors.initials: FX
authors.lastname: Ren
authors.affiliation: Shijingshan Center for Disease Control and Prevention, Beijing 100043, China; 5. Department of Epidemiology and Biostatistics, Peking University School of Public Health, Beijing 100191, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Shi
authors.affiliation: Peking University Clinical Research Institute, Beijing 100191, China; Department of Epidemiology and Biostatistics, Peking University School of Public Health, Beijing 100191, China; The George Institute, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Yang-feng
authors.initials: YF
authors.lastname: Wu
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001794:Blood Pressure; D002318:Cardiovascular Diseases / Q000453:epidemiology / Q000235:genetics*; D008076:Cholesterol, HDL / Q000097:blood; D016207:Cytokines; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics* / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000097:blood; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D009203:Myocardial Infarction; D050177:Overweight; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D020521:Stroke
chemical_list: D008076:Cholesterol, HDL; D016207:Cytokines; C508609:IL10 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D008077:Lipoproteins, LDL; D016753:Interleukin-10