title: Characteristics of fecal and mucosa-associated microbiota in Chinese patients with inflammatory bowel disease.
abstract: The intestinal microbiota plays an important role in the pathogenesis of inflammatory bowel disease (IBD), and geographical and genetic backgrounds impact the composition of the intestinal microbiota. However, there is a lack of evidence regarding the overall changes and characteristics of fecal-associated microbiota (FAM) and mucosa-associated microbiota (MAM) in Chinese patients with IBD. We recruited 26 patients with Crohn's disease (CD), 46 patients with ulcerative colitis (UC), and 21 healthy individuals; we collected matched fresh fecal and mucosal samples from the same subjects. The microbial communities were studied by 454-pyrosequencing. Community-wide changes in FAM and MAM were observed in patients with IBD. The proportion of several butyrate-producing bacteria, such as of the genera Roseburia, Coprococcus, and Ruminococcus were significantly reduced, whereas the pathogens Escherichia-Shigella and Enterococcus were prevalent in patients with IBD. FAM and MAM were similar between CD and UC. FAM differed from MAM in healthy individuals and patients with UC. In conclusion, the compositions of FAM and MAM were altered in patients with IBD. The reduction of butyrate-producing bacteria and the increase in opportunistic pathogens might be associated with the pathogenesis of IBD.
authors.affiliation: Department of Gastroenterology (LC, WW, RZ, JL, MH, FZ, BX), Zhongnan Hospital of Wuhan Univeristy; Hubei Clinical Center and Key Laboratory of Intestinal and Colorectal Disease (BX), Wuhan; Department of Medicine and Therapeutics (SCN), Li Ka Shing Institute of Health Science, Chinese University of Hong Kong, Hong Kong; Department of Gastroenterology (XW, KW), Xijing Hospital of the Fourth Military Medical University, Xi'an, China; Department of Gastroenterology/Hepatology (BS), The Cleveland Clinic Foundation, Cleveland, Ohio, USA; and Department of Gastroenterology (MAK), St Vincent's Hospital, Melbourne, Australia.
authors.forename: Liping
authors.initials: L
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Zhou
authors.forename: Siew C
authors.initials: SC
authors.lastname: Ng
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Meifang
authors.initials: M
authors.lastname: Huang
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Shen
authors.forename: Michael
authors.initials: M
authors.lastname: A Kamm
authors.forename: Kaichun
authors.initials: K
authors.lastname: Wu
authors.forename: Bing
authors.initials: B
authors.lastname: Xia
mesh_terms: D000293:Adolescent; D000328:Adult; D044466:Asian People; D002087:Butyrates / Q000378:metabolism; D003093:Colitis, Ulcerative / Q000382:microbiology*; D003424:Crohn Disease / Q000382:microbiology*; D016983:Enterococcus / Q000302:isolation & purification; D004925:Escherichia / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology*; D008297:Male; D064307:Microbiota; D008875:Middle Aged; D017422:Sequence Analysis, DNA; D012760:Shigella / Q000302:isolation & purification
chemical_list: D002087:Butyrates