title: Aberration correction during real time in vivo imaging of bone marrow with sensorless adaptive optics confocal microscope.
abstract: We have demonstrated adaptive correction of specimen-induced aberration during in vivo imaging of mouse bone marrow vasculature with confocal fluorescence microscopy. Adaptive optics system was completed with wavefront sensorless correction scheme based on stochastic parallel gradient descent algorithm. Using image sharpness as the optimization metric, aberration correction was performed based upon Zernike polynomial modes. The experimental results revealed the improved signal and resolution leading to a substantially enhanced image contrast with aberration correction. The image quality of vessels at 38- and 75-μm depth increased three times and two times, respectively. The corrections allowed us to detect clearer bone marrow vasculature structures at greater contrast and improve the signal-to-noise ratio.
authors.affiliation: Chinese Academy of Sciences, The Key Laboratory on Adaptive Optics, Chengdu 610209, ChinabChinese Academy of Sciences, Institute of Optics and Electronics, The Laboratory on Adaptive Optics, Chengdu 610209, ChinacUniversity of Chinese Academy of Sciences.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Shanghai Jiao Tong University, School of Biomedical Engineering and Med-X Research Institute, 1954 Huashan Road, Shanghai 200030, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wei
authors.affiliation: Chinese Academy of Sciences, The Key Laboratory on Adaptive Optics, Chengdu 610209, ChinabChinese Academy of Sciences, Institute of Optics and Electronics, The Laboratory on Adaptive Optics, Chengdu 610209, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wei
authors.affiliation: Chinese Academy of Sciences, The Key Laboratory on Adaptive Optics, Chengdu 610209, ChinabChinese Academy of Sciences, Institute of Optics and Electronics, The Laboratory on Adaptive Optics, Chengdu 610209, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: He
authors.affiliation: Chinese Academy of Sciences, The Key Laboratory on Adaptive Optics, Chengdu 610209, ChinabChinese Academy of Sciences, Institute of Optics and Electronics, The Laboratory on Adaptive Optics, Chengdu 610209, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Shi
authors.affiliation: Shanghai Jiao Tong University, School of Biomedical Engineering and Med-X Research Institute, 1954 Huashan Road, Shanghai 200030, China.
authors.forename: Xunbin
authors.initials: X
authors.lastname: Wei
authors.affiliation: Chinese Academy of Sciences, The Key Laboratory on Adaptive Optics, Chengdu 610209, ChinabChinese Academy of Sciences, Institute of Optics and Electronics, The Laboratory on Adaptive Optics, Chengdu 610209, China.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000465:Algorithms; D000818:Animals; D016477:Artifacts; D001853:Bone Marrow / Q000648:ultrastructure*; D003199:Computer Systems; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005246:Feedback; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation / Q000379:methods; D007909:Lenses; D051379:Mice; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity