title: Sequence specific detection of restriction enzymes at DNA-modified carbon nanotube field effect transistors.
abstract: Protein-DNA interactions play a central role in many cellular processes, and their misregulation has been implicated in a number of human diseases. Thus, there is a pressing need for the development of analytical strategies for interrogating the binding of proteins to DNA. Herein, we report the electrical monitoring of a prototypical DNA-binding protein, the PvuII restriction enzyme, at microfluidic-encapsulated, DNA-modified carbon nanotube field effect transistors. Our integrated platform enables the sensitive, sequence specific detection of PvuII at concentrations as low as 0.5 pM in a volume of 0.025 μL (corresponding to ~7500 proteins). These figures of merit compare favorably to state of the art values reported for alternative fluorescent and electrical assays. The overall detection strategy represents a step toward the massively parallel electrical monitoring, identification, and quantification of protein-DNA interactions at arrayed nanoscale devices.
authors.affiliation: Department of Chemical Engineering and Materials Science, University of California, Irvine , Irvine, California 92697, United States.
authors.forename: David D
authors.initials: DD
authors.lastname: Ordinario
authors.forename: Anthony M
authors.initials: AM
authors.lastname: Burke
authors.forename: Long
authors.initials: L
authors.lastname: Phan
authors.forename: Jonah-Micah
authors.initials: JM
authors.lastname: Jocson
authors.forename: Hanfei
authors.initials: H
authors.lastname: Wang
authors.forename: Mary N
authors.initials: MN
authors.lastname: Dickson
authors.forename: Alon A
authors.initials: AA
authors.lastname: Gorodetsky
mesh_terms: D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D015257:DNA-Cytosine Methylases / Q000032:analysis*; D004560:Electricity; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D014173:Transistors, Electronic
chemical_list: D037742:Nanotubes, Carbon; D009841:Oligonucleotides; D004247:DNA; C047084:DNA modification methylase PvuII; D015257:DNA-Cytosine Methylases