title: Potential usage for in vivo lectin screening in live animals utilizing cell surface mimetic glyco-nanoparticles, phosphorylcholine-coated quantum dots (PC-QDs).
abstract: Utilizing glycosylated derivatives as a tag, we are able to explore novel counter-receptor of endogenous lectins or lectin-like molecules in vivo. We have established the standardized methodology including preparation of glycosylated derivatives and construction of a platform for tracing the molecules in vivo at first. Combined use of an aminooxy-terminated thiol derivative and a phosphorylcholine (PC) derivative provides quantum dots (QDs) with novel functions for the chemical ligation of ketone-functionalized compounds and the prevention of nonspecific protein adsorption concurrently. In order to track the derivatives in vivo, near-infrared (NIR) fluorescence imaging of QDs displaying various simple sugars (glyco-PC-QDs) after administration into the tail vein of the mouse can be performed. It has revealed that distinct long-term delocalization over 2 h can be observed depending on the species of glycans ligated to PC-QDs at least in the liver. Until today we have performed live animal imaging utilizing various kinds of sialyl glyco-PC-QDs. They are still retained stably in whole body after 2 h while they showed significantly different in vivo dynamics in the tissue distribution, suggesting that structure/sequence of the neighboring sugar residues in the individual sialyl oligosaccharides might influence the final organ-specific distribution, which should be equivalent to the distribution of sialic acid-recognizing lectins. Here we describe a standardized protocol using ligand-displayed PC-QDs for live cell/animal imaging by versatile NIR fluorescence photometry without influence of size-dependent accumulation/excretion pathway for nanoparticles (e.g., viruses)>10 nm in hydrodynamic diameter by the liver.
authors.affiliation: Field of Drug Discovery Research, Faculty of Advanced Life Science, Hokkaido University, and Medicinal Chemistry Pharmaceuticals Co. INC, Sapporo, 001-0021, Japan, maho@sci.hokudai.ac.jp.
authors.forename: Maho
authors.initials: M
authors.lastname: Amano
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hinou
authors.forename: Risho
authors.initials: R
authors.lastname: Miyoshi
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Nishimura
mesh_terms: D000586:Amination; D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry*; D002470:Cell Survival; D037102:Lectins / Q000378:metabolism*; D051379:Mice; D061848:Optical Imaging / Q000379:methods*; D010767:Phosphorylcholine / Q000737:chemistry*; D010783:Photometry; D045663:Quantum Dots / Q000737:chemistry*
chemical_list: D037102:Lectins; D010767:Phosphorylcholine