title: Replication competent virus as an important source of bias in HIV latency models utilizing single round viral constructs.
abstract: The central memory T cell (TCM) model forms a unique HIV-1 latency model based on primary cells that closely resemble in vivo TCM. The virus employed in this model is based on an engineered vector incapable of replication after initial infection. We show that despite this strategy, replication competent viral particles are released into the culture medium due to recombination between overlapping sequences of the env deleted HIV genome that is co-transfected with intact env. This finding emphasizes the need for careful data analysis and interpretation if similar constructs are employed and urges for additional caution during laboratory work.
authors.forename: Pawel
authors.initials: P
authors.lastname: Bonczkowski
authors.forename: Ward
authors.initials: W
authors.lastname: De Spiegelaere
authors.forename: Alberto
authors.initials: A
authors.lastname: Bosque
authors.forename: Cory H
authors.initials: CH
authors.lastname: White
authors.forename: Anouk
authors.initials: A
authors.lastname: Van Nuffel
authors.forename: Eva
authors.initials: E
authors.lastname: Malatinkova
authors.forename: Maja
authors.initials: M
authors.lastname: Kiselinova
authors.forename: Wim
authors.initials: W
authors.lastname: Trypsteen
authors.forename: Wojciech
authors.initials: W
authors.lastname: Witkowski
authors.forename: Jolien
authors.initials: J
authors.lastname: Vermeire
authors.forename: Bruno
authors.initials: B
authors.lastname: Verhasselt
authors.forename: Laura
authors.initials: L
authors.lastname: Martins
authors.forename: Christopher H
authors.initials: CH
authors.lastname: Woelk
authors.forename: Vicente
authors.initials: V
authors.lastname: Planelles
authors.forename: Linos
authors.initials: L
authors.lastname: Vandekerckhove
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D015752:Genes, env; D005822:Genetic Vectors / Q000235:genetics; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D017735:Virus Latency; D014779:Virus Replication / Q000235:genetics*
chemical_list: D004279:DNA, Viral