title: Detection of organochlorine compounds formed during the contact of sodium hypochlorite with dentin and dental pulp.
abstract: This study used gas chromatography-mass spectrometry (GC-MS) to detect the products formed during the contact of sodium hypochlorite (NaOCl) with bovine pulp and dentin. For analysis of the products formed in the volatile phase, 11 mg of bovine pulp tissue were placed in contact with 0.5%, 2.5% and 5.25% NaOCl until complete tissue dissolution occurred. The solid phase microextraction (SPME) fiber was exposed inside the container through the cover membrane and immediately injected into the GC-MS system. 30 mg of the of dentin were kept in contact with NaOCl, and then the SPME fiber was exposed inside the container through the cover membrane for adsorption of the products and injected into the GC-MS system. The same protocol was used for the aqueous phase. For analysis of the volatile compounds, the final solution was extracted using pure ethyl ether. The suspended particulate phase of the mixture was aspirated, and ether was separated from the aqueous phase of the solution. The ether containing the products that resulted from the chemical interaction of dentin and pulp with the NaOCl was filtered and then injected into the GC-MS system for analysis of the aqueous phase. The aqueous and volatile phases of both dentin and pulp showed the formation of chloroform, hexachloroethane, dichloromethylbenzene and benzaldehyde. In conclusion, organochlorine compounds are generated during the contact of dentin and pulp with NaOCl at concentrations of 0.5%, 2.5% and 5.25%.
authors.affiliation: Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Tiago Gilioli
authors.initials: TG
authors.lastname: Varise
authors.affiliation: Federal University of Goiás, Goiânia, GO, Brazil.
authors.forename: Carlos
authors.initials: C
authors.lastname: Estrela
authors.affiliation: Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Débora Fernandes Costa
authors.initials: DF
authors.lastname: Guedes
authors.affiliation: Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Manoel Damião
authors.initials: MD
authors.lastname: Sousa-Neto
authors.affiliation: Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Jesus Djalma
authors.initials: JD
authors.lastname: Pécora
mesh_terms: D000818:Animals; D002417:Cattle; D003782:Dental Pulp / Q000737:chemistry*; D003804:Dentin / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006843:Hydrocarbons, Chlorinated / Q000032:analysis*; D012973:Sodium Hypochlorite / Q000737:chemistry*; D052617:Solid Phase Microextraction
chemical_list: D006843:Hydrocarbons, Chlorinated; D012973:Sodium Hypochlorite