title: Changes in biochemical characteristics and activities of ripening associated enzymes in mango fruit during the storage at different temperatures.
abstract: As a part of the study to explore the possible strategy for enhancing the shelf life of mango fruits, we investigated the changes in biochemical parameters and activities of ripening associated enzymes of Ashwina hybrid mangoes at 4-day regular intervals during storage at -10°C, 4°C, and 30 ± 1°C. Titratable acidity, vitamin C, starch content, and reducing sugar were higher at unripe state and gradually decreased with the increasing of storage time at all storage temperatures while phenol content, total soluble solid, total sugar, and nonreducing sugar contents gradually increased. The activities of amylase, α-mannosidase, α-glucosidase, and invertase increased sharply within first few days and decreased significantly in the later stage of ripening at 30 ± 1°C. Meanwhile polyphenol oxidase, β-galactosidase, and β-hexosaminidase predominantly increased significantly with the increasing days of storage till later stage of ripening. At -10°C and 4°C, the enzymes as well as carbohydrate contents of storage mango changed slightly up to 4 days and thereafter the enzyme became fully dormant. The results indicated that increase in storage temperature and time correlated with changes in biochemical parameters and activities of glycosidases suggested the suppression of β-galactosidase and β-hexosaminidase might enhance the shelf life of mango fruits.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh ; Genetic Engineering Laboratory, Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
authors.forename: Md Anowar
authors.identifier: 0000-0003-3967-2544
authors.initials: MA
authors.lastname: Hossain
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh.
authors.forename: Md Masud
authors.identifier: 0000-0003-3635-2166
authors.initials: MM
authors.lastname: Rana
authors.affiliation: Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Kimura
authors.affiliation: Genetic Engineering Laboratory, Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
authors.forename: Hairul Azman
authors.identifier: 0000-0002-6700-6138
authors.initials: HA
authors.lastname: Roslan
mesh_terms: D002678:Chimera / Q000378:metabolism*; D005638:Fruit / Q000201:enzymology*; D031022:Mangifera / Q000201:enzymology*; D010940:Plant Proteins / Q000378:metabolism*; D013997:Time Factors
chemical_list: D010940:Plant Proteins