title: Green synthesis of gold nanoparticles for staining human cervical cancer cells and DNA binding assay.
abstract: Gold nanoparticles have been functionalized by non-ionic surfactants (polysorbates) used in pharmaceutical formulations. This results in the formation of more well-dispersed gold nanoparticles (GNPs) than the GNPs formed in neat water. The synthesized GNPs show good temporal stability. The synthesis conditions are mild and environmentally benign. The GNPs can bind to ct-DNA and displace bound dye molecules. The DNA-binding assay is significant as it preliminarily indicated that DNA-GNP conjugates can be formed. Such conjugates are extremely promising for applications in nanobiotechnology. The GNPs can also stain the human cervical cancer (HeLa) cells over a wide concentration range while remaining non-cytotoxic, thus providing a non invasive cell staining method. This result is very promising as we observe staining of HeLa cells at very low GNP concentrations (1 μM) while the cell viability is retained even at 10-fold higher GNP concentrations.
authors.affiliation: Department of Chemistry, University of Kalyani, West Bengal 741 235, India. Electronic address: deswati1@gmail.com.
authors.forename: Swati
authors.initials: S
authors.lastname: De
authors.affiliation: Department of Chemistry, University of Kalyani, West Bengal 741 235, India.
authors.forename: Rikta
authors.initials: R
authors.lastname: Kundu
authors.affiliation: Department of Bio-chemistry and Biophysics, University of Kalyani, West Bengal 741 235, India.
authors.forename: Atanu
authors.initials: A
authors.lastname: Ghorai
authors.affiliation: Department of Chemistry, University of Kalyani, West Bengal 741 235, India.
authors.forename: Ranju Prasad
authors.initials: RP
authors.lastname: Mandal
authors.affiliation: Department of Bio-chemistry and Biophysics, University of Kalyani, West Bengal 741 235, India.
authors.forename: Utpal
authors.initials: U
authors.lastname: Ghosh
mesh_terms: D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006046:Gold / Q000737:chemistry; D055772:Green Chemistry Technology; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity; D008823:Micelles; D008856:Microscopy, Fluorescence; D020349:Surface Plasmon Resonance; D014466:Ultraviolet Rays; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology
chemical_list: D008823:Micelles; D006046:Gold; D004247:DNA; C427203:calf thymus DNA
keywords: Cell staining; Cytotoxicity; DNA; Gold nanoparticle; Polysorbate