title: Preventive use of Bach flower Rescue Remedy in the control of risk factors for cardiovascular disease in rats.
abstract: OBJECTIVE
  To evaluate the effect of Bach flower Rescue Remedy on the control of risk factors for cardiovascular disease in rats.
  
  
  METHODS
  A randomized longitudinal experimental study.
  
  
  METHODS
  Eighteen Wistar rats were randomly divided into three groups of six animals each and orogastrically dosed with either 200 μl of water (group A, control), or 100 μl of water and 100 μl of Bach flower remedy (group B), or 200 μl of Bach flower remedy (group C) every 2 days, for 20 days. All animals were fed standard rat chow and water ad libitum.
  
  
  METHODS
  Urine volume, body weight, feces weight, and food intake were measured every 2 days. On day 20, tests of glycemia, hyperuricemia, triglycerides, high-density lipoprotein (HDL) cholesterol, and total cholesterol were performed, and the anatomy and histopathology of the heart, liver and kidneys were evaluated. Data were analyzed using Tukey's test at a significance level of 5%.
  
  
  RESULTS
  No significant differences were found in food intake, feces weight, urine volume and uric acid levels between groups. Group C had a significantly lower body weight gain than group A and lower glycemia compared with groups A and B. Groups B and C had significantly higher HDL-cholesterol and lower triglycerides than controls. Animals had mild hepatic steatosis, but no cardiac or renal damage was observed in the three groups.
  
  
  CONCLUSIONS
  Bach flower Rescue Remedy was effective in controlling glycemia, triglycerides, and HDL-cholesterol and may serve as a strategy for reducing risk factors for cardiovascular disease in rats. This study provides some preliminary "proof of concept" data that Bach Rescue Remedy may exert some biological effects.
authors.affiliation: Universidade do Vale do Sapucaí (UNIVÁS), Pouso Alegre, MG, Brazil. Electronic address: ccalafiori@uol.com.br.
authors.forename: Margarida Maria de Carvalho
authors.initials: MM
authors.lastname: Resende
authors.affiliation: Instituto Nacional de Telecomunicação, Santa Rita do Sapucaí, MG, Brazil.
authors.forename: Francisco Eduardo de Carvalho
authors.initials: FE
authors.lastname: Costa
authors.affiliation: Universidade do Vale do Sapucaí (UNIVÁS), Pouso Alegre, MG, Brazil.
authors.forename: Rodrigo Galvão Bueno
authors.initials: RG
authors.lastname: Gardona
authors.affiliation: Universidade do Vale do Sapucaí (UNIVÁS), Pouso Alegre, MG, Brazil.
authors.forename: Rochilan Godinho
authors.initials: RG
authors.lastname: Araújo
authors.affiliation: Universidade do Vale do Sapucaí (UNIVÁS), Pouso Alegre, MG, Brazil.
authors.forename: Fiorita Gonzales Lopes
authors.initials: FG
authors.lastname: Mundim
authors.affiliation: Universidade Federal da Paraíba, João Pessoa, PB, Brazil.
authors.forename: Maria José de Carvalho
authors.initials: MJ
authors.lastname: Costa
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects*; D002318:Cardiovascular Diseases; D002784:Cholesterol / Q000097:blood*; D004435:Eating / Q000187:drug effects; D065829:Flower Essences / Q000008:administration & dosage / Q000494:pharmacology*; D008137:Longitudinal Studies; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012307:Risk Factors
chemical_list: D001786:Blood Glucose; D065829:Flower Essences; D002784:Cholesterol
keywords: Animal experimentation; Bach flowers; Complementary therapies; Preventive medicine; Wistar rats