title: Photodynamic therapy using systemic administration of 5-aminolevulinic acid and a 410-nm wavelength light-emitting diode for methicillin-resistant Staphylococcus aureus-infected ulcers in mice.
abstract: Bacterial resistance to antibiotics has become a worldwide problem. One potential alternative for bacterial control is photodynamic therapy. 5-aminolevulinic acid is a natural precursor of the photosensitizer protoporphyrin IX. Relatively little is known about the antibacterial efficacy of photodynamic therapy using the systemic administration of 5-aminolevulinic acid; a few reports have shown that 5-aminolevulinic acid exerts photodynamic effects on methicillin-resistant Staphylococcus aureus (MRSA) in vitro. In this study, we evaluated the effectiveness of photodynamic therapy using 5-aminolevulinic acid and a 410-nm wavelength light-emitting diode in vitro and in vivo for the treatment of MRSA. We found that 5-aminolevulinic acid photodynamic therapy with the light-emitting diode had an in-vitro bactericidal effect on MRSA. In vivo, protoporphyrin IX successfully accumulated in MRSA on ulcer surfaces after intraperitoneal administration of 5-aminolevulinic acid to mice. Furthermore, 5-aminolevulinic acid photodynamic therapy accelerated wound healing and decreased bacterial counts on ulcer surfaces; in contrast, vancomycin treatment did not accelerate wound healing. Our findings indicate that 5-aminolevulinic acid photodynamic therapy may be a new treatment option for MRSA-infected wounds.
authors.affiliation: Department of Dermatology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors.forename: Kuniyuki
authors.initials: K
authors.lastname: Morimoto
authors.affiliation: Department of Dermatology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Ozawa
authors.affiliation: Medical Beam Physics Laboratory, Osaka University Graduate School of Engineering, Osaka, Japan.
authors.forename: Kunio
authors.initials: K
authors.lastname: Awazu
authors.affiliation: Medical Beam Physics Laboratory, Osaka University Graduate School of Engineering, Osaka, Japan.
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Ito
authors.affiliation: Medical Beam Physics Laboratory, Osaka University Graduate School of Engineering, Osaka, Japan.
authors.forename: Norihiro
authors.initials: N
authors.lastname: Honda
authors.affiliation: Department of Bacteriology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors.forename: Sohkichi
authors.initials: S
authors.lastname: Matsumoto
authors.affiliation: Department of Dermatology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tsuruta
mesh_terms: D000622:Aminolevulinic Acid / Q000008:administration & dosage*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D008027:Light; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D013203:Staphylococcal Infections / Q000188:drug therapy*; D014456:Ulcer / Q000188:drug therapy*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D017319:Photosensitizing Agents; D000622:Aminolevulinic Acid