title: Krüppel-like factor 5 as potential molecular marker in cervical cancer and the KLF family profile expression.
abstract: Cervical cancer (CC) as other cancer types, presents molecular deregulations, such as the alterations of transcription factors. Krüppel-like factors (KLF) are a family of transcriptional regulators. They are involved in diverse cellular processes, such as proliferation, apoptosis, and angiogenesis among others. Here, we analyzed the expression of all 17 KLF members at messenger RNA (mRNA) level, and protein expression of the two most commonly altered KLF5 and KLF6 in cervical tissues. Fifty-nine cervical tissues ranging from normal tissue to CC were evaluated for KLF1-17 mRNA expression by end-point RT-PCR and KLF5 by qRT-PCR. For KLF5 and KLF6 protein analysis, a tissue microarray was constructed containing the 59 cases and subjected for immunohistochemistry assay and KLF6 IVS1-27G>A single nucleotide polymorphism by direct DNA sequencing. KLF2-16 expressions were present in normal tissue, whereas all 17 were present in Low-Grade Squamous Intraepithelial Lesion, High-Grade-SIL and CC, unrelated to presence of human papillomavirus (HPV). KLF5 mRNA expression gradually increased throughout the subgroups and overexpressed in CC (p=0.01). KLF5 and KLF6 proteins were immunodetected in all samples. For the KLF6 SNP analysis, 80% of the CC population analyzed presented GG genotype and the remaining 20% presented GA genotype (p=0.491). Our present data show that KLFs expression could not be related to HPV presence, at least at transcriptional level, and KLF5 mRNA overexpression could represent a potential molecular marker for CC; KLF6 SNP has no relation to increased risk of CC.
authors.affiliation: Laboratorio de Oncología Genómica, Unidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, CMN-SXXI. IMSS, Av. Cuauhtémoc 330, Col. Doctores, México, D.F., 06720, Mexico.
authors.forename: Daniel
authors.initials: D
authors.lastname: Marrero-Rodríguez
authors.forename: Keiko
authors.initials: K
authors.lastname: Taniguchi-Ponciano
authors.forename: Florinda
authors.initials: F
authors.lastname: Jimenez-Vega
authors.forename: Pablo
authors.initials: P
authors.lastname: Romero-Morelos
authors.forename: Monica
authors.initials: M
authors.lastname: Mendoza-Rodríguez
authors.forename: Alejandra
authors.initials: A
authors.lastname: Mantilla
authors.forename: Miriam
authors.initials: M
authors.lastname: Rodriguez-Esquivel
authors.forename: Daniel
authors.initials: D
authors.lastname: Hernandez
authors.forename: Angeles
authors.initials: A
authors.lastname: Hernandez
authors.forename: Guillermo
authors.initials: G
authors.lastname: Gomez-Gutierrez
authors.forename: Nancy
authors.initials: N
authors.lastname: Muñoz-Hernandez
authors.forename: Hugo Arreola-de
authors.initials: HA
authors.lastname: la Cruz
authors.forename: Claudia
authors.initials: C
authors.lastname: Vargas-Requena
authors.forename: Cecilia
authors.initials: C
authors.lastname: Díaz-Hernández
authors.forename: Luis
authors.initials: L
authors.lastname: Serna-Reyna
authors.forename: Marco
authors.initials: M
authors.lastname: Meraz-Rios
authors.forename: Cindy
authors.initials: C
authors.lastname: Bandala
authors.forename: Jorge
authors.initials: J
authors.lastname: Ortiz-Leon
authors.forename: Mauricio
authors.initials: M
authors.lastname: Salcedo
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D002584:Cervix Uteri / Q000378:metabolism / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011379:Prognosis; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C492214:KLF5 protein, human; C105465:KLF6 protein, human; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger