title: Electrochemical performance and detection of 8-Hydroxy-2'-deoxyguanosine at single-stranded DNA functionalized graphene modified glassy carbon electrode.
abstract: 8-Hydroxy-2'-deoxyguanosine (8-OH-dG) is the most abundant oxidative product of DNA and it is also considered to be a biomarker of oxidative DNA damage. In this work, a novel biosensor was fabricated by combining the biocompatibility of single-stranded DNA (ss-DNA) and the excellent conductivity of graphene nanosheets (GNs). The biosensor showed high electrocatalytic activity to the oxidation of 8-OH-dG. The sensitivities were 13.23 (±0.03)μA μM(-1), 5.827 (±0.008) μA μM(-1) and 3.086 (±0.005) μA μM(-1) in the concentration ranges of 0.0056-1.155 μM, 1.155-11.655 μM and 11.655-36.155 μM, respectively. The detection limit was 0.875 nM (S/N=3). Furthermore, the electrochemical behavior of 8-OH-dG was also investigated in detail in the presence of uric acid. And the results showed that uric acid had interference with the trace detection of 8-OH-dG. But it can be eliminated completely by uricase. The as-prepared ss-DNA/GNs/GCE will be promising for the detection of 8-OH-dG in real samples.
authors.affiliation: Department of Chemistry, Liaocheng University, Liaocheng, Shandong 252059, PR China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Jia
authors.affiliation: Department of Chemistry, Liaocheng University, Liaocheng, Shandong 252059, PR China.
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Liu
authors.affiliation: Department of Chemistry, Liaocheng University, Liaocheng, Shandong 252059, PR China. Electronic address: hswang@lcu.edu.cn.
authors.forename: Huai-Sheng
authors.initials: HS
authors.lastname: Wang
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D015374:Biosensing Techniques / Q000295:instrumentation*; D002244:Carbon / Q000737:chemistry; D003217:Conductometry / Q000295:instrumentation*; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000652:urine*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics / Q000652:urine; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D044367:Phase Transition; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002244:Carbon; D006108:Graphite; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine
keywords: 8-Hydroxy-2′-deoxyguanosine; Graphene; Single-stranded DNA; Uric acid