title: Distribution and heterogeneity of heterochromatin in the European huchen (Hucho hucho Linnaeus, 1758) (Salmonidae).
abstract: The chromosomal characteristics, locations and variations of the heterochromatin were studied in the European huchen (Hucho hucho, Linnaeus, 1758) karyotype using conventional C- banding, endonuclease digestion banding, silver nitrate (AgNO3), chromomycin A3 (CMA3) and DAPI staining techniques. The karyotype consists of 82 chromosomes: 13 pairs of metacentric chromosomes, 2 pairs of submetacentric chromosomes and 26 pairs of subtelo-acrocentric chromosomes (NF = 112). Original data on the chromosomal distribution of segments resistant to Alu I, Dde I and Mbo I restriction endonucleases and identification of the C-banded heterochromatin presented here have been used to characterize the huchen karyotype. On the basis of the banding patterns provided in the course of restriction enzyme digestion, AgNO3/CMA3 staining and C-banding we distinguished twelve types of heterochromatin grouped in four areas of the European huchen chromosomes. One pair of NOR-bearing chromosomes was found to be polymorphic in size and displayed two distinct forms. A combination of various staining techniques provided original data concerning heterochromatin distribution and heterogeneity in the European huchen which enabled better karyotypic definition of this fish species.
authors.forename: Kucinski
authors.initials: K
authors.lastname: Marcin
authors.forename: Ocalewicz
authors.initials: O
authors.lastname: Konrad
authors.forename: Fopp-Bayat
authors.initials: FB
authors.lastname: Dorota
authors.forename: Liszewski
authors.initials: L
authors.lastname: Tomasz
authors.forename: Furgala-Selezniow
authors.initials: FS
authors.lastname: Grazyna
authors.forename: Jankun
authors.initials: J
authors.lastname: Malgorzata
mesh_terms: D000818:Animals; D002875:Chromosomes; D006570:Heterochromatin / Q000235:genetics*; D007621:Karyotyping; D012487:Salmonidae / Q000235:genetics
chemical_list: D006570:Heterochromatin