title: Anti-inflammatory activity of methanol extract and n-hexane fraction mojabanchromanol b from Myagropsis myagroides.
abstract: OBJECTIVE
  This study was carried out to verify the anti-inflammatory effect of methanol extract from Myagropsis myagroides (MMME) and its n-hexane fraction mojabanchromanol b.
  
  
  METHODS
  The murine macrophages Raw264.7 cells were used. The pro-inflammatory cytokines (IL-6, IL-1β, TNF-α) and the expression of iNOS, COX-2, and NF-κB p65 were examined by ELISA and immunoblotting. To investigate the inhibitory effect of MMME in an animal model of inflammation, an assay to determine croton oil-induced ear edema in mice was performed.
  
  
  RESULTS
  NO levels decreased with increasing concentration of MMME, and were inhibited up to 50%. The secretion of IL-6, TNF-α, and IL-1β was suppressed in a dose-dependent manner, especially at 50μg/mL, inhibition activities of cytokines were over 50%. MMME also suppressed the expression of COX-2, iNOS, and NF-κB p65, suggesting that MMME could affect the expression of inflammation related cytokines and proteins through the deregulation of NF-κB. Moreover, the formation of mouse ear edema was reduced at the highest dose tested compared to that in the control, and generated similar effects compared with prednisolone at 250mg/kg in mice ear edema evaluation test. In addition, the results in photomicrograph of mice ear tissue and mast cells also showed the same effect. After purification of fractions of MMME, it indicated that n-hexane fraction mojabanchromanol b was the most active fraction showing the inhibitory effect of IL-6 and TNF-α.
  
  
  CONCLUSIONS
  These results suggested that MMME and mojabanchromanol b may have great effects on inflammatory factors and be potential anti-inflammatory therapeutic materials.
authors.affiliation: Department of Food Science & Technology/Institute of Food Science, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Da-Hyun
authors.initials: DH
authors.lastname: Jeong
authors.affiliation: Institute of Fisheries Sciences, Pukyong National University, Busan 619-911, Republic of Korea.
authors.forename: Koth-Bong-Woo-Ri
authors.initials: KB
authors.lastname: Kim
authors.affiliation: Institute of Fisheries Sciences, Pukyong National University, Busan 619-911, Republic of Korea.
authors.forename: Min-Ji
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Department of Food Science & Technology/Institute of Food Science, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Bo-Kyeong
authors.initials: BK
authors.lastname: Kang
authors.affiliation: Department of Food Science & Technology/Institute of Food Science, Pukyong National University, Busan 608-737, Republic of Korea. Electronic address: dhahn@pknu.ac.kr.
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Ahn
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D002839:Chromans; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000188:drug therapy / Q000473:pathology; D005419:Flavonoids / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006586:Hexanes / Q000737:chemistry; D007249:Inflammation / Q000188:drug therapy* / Q000473:pathology; D018836:Inflammation Mediators / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D000432:Methanol / Q000737:chemistry; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000378:metabolism; D000459:Phaeophyceae / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D002839:Chromans; D016207:Cytokines; D005419:Flavonoids; D006586:Hexanes; D018836:Inflammation Mediators; D010936:Plant Extracts; C000593004:mojabanchromanol b; C026385:n-hexane; D009569:Nitric Oxide; D000432:Methanol
keywords: Anti-inflammatory; Mice ear edema; Myagropsis myagroides; Nuclear factor kappa B p65; n-Hexane fraction