title: Functional brush-decorated poly(globalide) films by ARGET-ATRP for bioconjugation.
abstract: Formation of hydroxyl functionalized poly(macrolactone) films was achieved by a combination of enzymatic ring opening polymerization of globalide and thiol-ene coupling reactions. Reaction with α-bromoisobutyryl bromide yielded ATRP initiator functional films. Efficient activator regeneration by electron transfer (ARGET) ATRP was employed for the polymerization of tert-butyl acrylate (tBA) and NIPAM from the initiator decorated surfaces. Deprotection of the tert-butyl ester decorated polymer films yielded carboxylic acid groups for convenient attachment of biomolecule. The successful conjugation of green fluorescent protein (eGFP) and active enzyme chitobiase (Chb) on densely poly(acrylic acid)-functionalized polyester films is demonstrated.
authors.affiliation: School of Chemical Sciences, Dublin City University, Dublin 9, Ireland.
authors.forename: Zeliha
authors.initials: Z
authors.lastname: Ates
authors.forename: Fabrice
authors.initials: F
authors.lastname: Audouin
authors.forename: Amy
authors.initials: A
authors.lastname: Harrington
authors.forename: Brendan
authors.initials: B
authors.lastname: O'Connor
authors.forename: Andreas
authors.initials: A
authors.lastname: Heise
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000179:Acrylates / Q000737:chemistry; D000180:Acrylic Resins / Q000138:chemical synthesis / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D004583:Electrons; D057927:Hydrophobic and Hydrophilic Interactions; D008856:Microscopy, Fluorescence; D011091:Polyesters / Q000737:chemistry*; D058105:Polymerization; D013055:Spectrophotometry, Infrared; D013696:Temperature
chemical_list: D000179:Acrylates; D000180:Acrylic Resins; D003432:Cross-Linking Reagents; D011091:Polyesters; C006903:carbopol 940; C522411:tert-butyl acrylate; D000118:Acetylglucosaminidase
keywords: atom transfer radical polymerization (ATRP); bioconjugation; macrolactones; polyester films; polymer brushes