title: Activation effects of polysaccharides of Flammulina velutipes mycorrhizae on the T lymphocyte immune function.
abstract: Flammulina velutipes mycorrhizae have increasingly been produced with increasing of F. velutipes production. A mouse model was thus used to examine potential effect of F. velutipes mycorrhizae on the immune function. Fifty female Wistar mice (5-weeks-old) weighed 15-20 g were randomly allocated into five groups. Polysaccharide of F. velutipes mycorrhizae were treated with mice and mice spleen lymphocytes. The levels of CD3(+), CD4(+), and CD8(+) T lymphocyte, interleukin-2 (IL-2), and tumor necrosis factor-a (TNF-α) were determined. The results showed that the proportions of CD3(+), and CD4(+) T lymphocyte, the ratio of CD4(+)/CD8(+), and the levels of IL-2 and TNF-a were significantly increased in polysaccharide of F. velutipes mycorrhizae, while the proportion of CD8(+) T lymphocyte was decreased in polysaccharide of F. velutipes mycorrhizae-dose dependent manner. Our findings indicated that a long term exposure of polysaccharide of F. velutipes mycorrhizae could activate the T lymphocyte immune function. Polysaccharide of F. velutipes mycorrhizae was expected to develop into the immune health products.
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Zheng-Fei
authors.initials: ZF
authors.lastname: Yan
authors.affiliation: Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Nai-Xu
authors.identifier: 0000-0002-2762-6885
authors.initials: NX
authors.lastname: Liu
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Xin-Xin
authors.initials: XX
authors.lastname: Mao
authors.affiliation: Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Yu
authors.identifier: 0000-0002-8937-0416
authors.initials: Y
authors.lastname: Li
authors.affiliation: Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Chang-Tian
authors.identifier: 0000-0001-6498-8642
authors.initials: CT
authors.lastname: Li
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002470:Cell Survival; D005260:Female; D055393:Flammulina / Q000276:immunology*; D062610:Fungal Polysaccharides / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D007376:Interleukin-2 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D013154:Spleen / Q000033:anatomy & histology / Q000166:cytology / Q000187:drug effects; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D060168:Thymocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D013950:Thymus Gland / Q000033:anatomy & histology / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D062610:Fungal Polysaccharides; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha