title: Prevalence and clinical utility of human papilloma virus genotyping in patients with cervical lesions.
abstract: OBJECTIVE
  Cervical cancer is the commonest cancer among Indian women. High-risk human papilloma virus (HPV) detection holds the potential to be used as a tool to identify women, at risk of subsequent development of cervical cancer. There is a pressing need to identify prevalence of asymptomatic cervical HPV infection in local population. In our study, we explored the prevalence of HPV genotypes and their distribution in women with cervical lesions.
  
  
  METHODS
  Scrape specimens were obtained from 100 women (study group) with cervical abnormalities. HPV was detected with amplicor HPV tests, and the individual genotypes in these specimens were identified by Hybribio Genoarray test kit. Fifty specimens were also collected from females with healthy cervix (control group). The present study also aimed to determine the status of HPV prevalence and its association with different sociodemographic factors.
  
  
  RESULTS
  Out of the total number of 100 samples, 10 (10 %) women tested positive for HPV DNA. Among them, HPV 18 was observed in 6, HPV 16 in 2, HPV 52 and HPV 39 in one each. Fifty specimens collected from patients with healthy cervix were not infected with any of the HPV genotype.
  
  
  CONCLUSIONS
  Our study generates data of HPV prevalence in patients with cervical lesions visiting tertiary care institute. The data generated will be useful for laying guidelines for mass screening of HPV detection, treatment, and prophylaxis.
authors.affiliation: Department of Microbiology, Sri Guru Ram Das Institute of Medical Sciences and Research, Amritsar, India ; H. No. 1658, Phase 7, Mohali, 160062 Punjab India.
authors.forename: Parminder
authors.initials: P
authors.lastname: Kaur
authors.affiliation: Department of Microbiology, Sri Guru Ram Das Institute of Medical Sciences and Research, Amritsar, India.
authors.forename: Aruna
authors.initials: A
authors.lastname: Aggarwal
authors.affiliation: Department of Obstetrics and Gynaecology, Sri Guru Ram Das Institute of Medical Sciences and Research, Amritsar, India.
authors.forename: Madhu
authors.initials: M
authors.lastname: Nagpal
authors.affiliation: Department of Microbiology, Sri Guru Ram Das Institute of Medical Sciences and Research, Amritsar, India.
authors.forename: Loveena
authors.initials: L
authors.lastname: Oberoi
authors.affiliation: Department of Obstetrics and Gynaecology, Sri Guru Ram Das Institute of Medical Sciences and Research, Amritsar, India.
authors.forename: Swati
authors.initials: S
authors.lastname: Sharma
keywords: Cervical cancer; Genotyping; Human papilloma virus