title: Combined use of NGF/BDNF/bFGF promotes proliferation and differentiation of neural stem cells in vitro.
abstract: Neurotrophic factors can promote the proliferation and differentiation of neural stem cells (NSCs). Here we report that the possibility of using bFGF in combination with BDNF and NGF to promote proliferation and differentiation of NSCs in vitro. C57BL/6 mouse NSCs were cultured, passaged and stained by immunofluorescence for nestin and GFP. According to different neurotrophic factors added to NSCs, seven experiment groups (NGF, BDNF, bFGF, bFGF+NGF, bFGF+BDNF, NGF+BDNF and NGF+BDNF+bFGF) and a blank control group were established. One week after induction and differentiation, results showed that there was significant difference in the percentage of NSCs differentiating into neurons among the experiment groups. The percentage in the multi-factor groups was significantly higher than that in the single-factor groups (p<0.05), among which the percentage was the highest in NGF+BDNF+bFGF group. In the two-factor groups, the percentage in bFGF+NGF and bFGF+BDNF groups was significantly higher than that in NGF+BDNF group (p<0.05). The NSCs growth curves showed that cells proliferated continuously with the time of culture prolonging, but there was significant difference between the group containing bFGF and that without bFGF. Our results demonstrate that combined use of NGF/BDNF/bFGF significantly improved the ability of NSCs proliferation and differentiation.
authors.affiliation: Neuroimaging Research Center, The Affiliated Suzhou Hospital, Nanjing Medical University, Suzhou 215001, China. Electronic address: sznaonao@163.com.
authors.forename: Shuang-Qing
authors.initials: SQ
authors.lastname: Chen
authors.affiliation: Neuroimaging Research Center, The Affiliated Suzhou Hospital, Nanjing Medical University, Suzhou 215001, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Neuroimaging Research Center, The Affiliated Suzhou Hospital, Nanjing Medical University, Suzhou 215001, China.
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Shen
authors.affiliation: Department of Neurology, First Hospital of Soochow University, Suzhou 215001, China.
authors.forename: Xiu-Ying
authors.initials: XY
authors.lastname: Cai
authors.affiliation: Department of Neurology, First Hospital of Soochow University, Suzhou 215001, China.
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Lei
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004338:Drug Combinations; D004622:Embryo, Mammalian; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D020932:Nerve Growth Factor / Q000494:pharmacology; D009414:Nerve Growth Factors / Q000494:pharmacology*; D058953:Neural Stem Cells / Q000187:drug effects*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D013997:Time Factors
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D004338:Drug Combinations; D005904:Glial Fibrillary Acidic Protein; D009414:Nerve Growth Factors; D005346:Fibroblast Growth Factors; D020932:Nerve Growth Factor; D010751:Phosphopyruvate Hydratase
keywords: Basic fibroblast growth factor (bFGF); Brain-derived neurotrophic factor (BDNF); Differentiation; Nerve growth factor (NGF); Neural stem cells (NSCs)