title: Dengue virus 2 American-Asian genotype identified during the 2006/2007 outbreak in Piauí, Brazil reveals a Caribbean route of introduction and dissemination of dengue virus in Brazil.
abstract: Dengue virus (DENV) is the most widespread arthropod-borne virus, and the number and severity of outbreaks has increased worldwide in recent decades. Dengue is caused by DENV-1, DENV- 2, DENV-3 and DENV-4 which are genetically distant. The species has been subdivided into genotypes based on phylogenetic studies. DENV-2, which was isolated from dengue fever patients during an outbreak in Piaui, Brazil in 2006/2007 was analyzed by sequencing the envelope (E) gene. The results indicated a high similarity among the isolated viruses, as well as to other DENV-2 from Brazil, Central America and South America. A phylogenetic and phylogeographic analysis based on DENV-2E gene sequences revealed that these viruses are grouped together with viruses of the American-Asian genotype in two distinct lineages. Our results demonstrate the co-circulation of two American-Asian genotype lineages in northeast Brazil. Moreover, we reveal that DENV-2 lineage 2 was detected in Piauí before it disseminated to other Brazilian states and South American countries, indicating the existence of a new dissemination route that has not been previously described.
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Leandra
authors.initials: L
authors.lastname: Barcelos Figueiredo
authors.affiliation: Laboratório de Biodados, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Tetsu
authors.initials: T
authors.lastname: Sakamoto
authors.affiliation: Laboratório de Vacinas, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brazil.
authors.forename: Luiz Felipe
authors.initials: LF
authors.lastname: Leomil Coelho
authors.affiliation: Laboratório de Vírus, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Eliseu Soares
authors.initials: ES
authors.lastname: de Oliveira Rocha
authors.affiliation: Laboratório de Vírus, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Marcela Menezes
authors.initials: MM
authors.lastname: Gomes Cota
authors.affiliation: Laboratório de Biotecnologia, Universidade Federal do Piauí, Parnaíba, Piauí, Brazil.
authors.forename: Gustavo Portela
authors.initials: GP
authors.lastname: Ferreira
authors.affiliation: Laboratório de Imunologia Celular e Molecular, Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Jaquelline Germano
authors.initials: JG
authors.lastname: de Oliveira
authors.affiliation: Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Erna Geessien
authors.initials: EG
authors.lastname: Kroon
mesh_terms: D000293:Adolescent; D000328:Adult; D000330:Aedes; D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D017691:Caribbean Region / Q000453:epidemiology; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000235:genetics; D003715:Dengue / Q000453:epidemiology / Q000635:transmission* / Q000821:virology; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004196:Disease Outbreaks; D005260:Female; D014644:Genetic Variation; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D007223:Infant; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D058974:Phylogeography; D020641:Polymorphism, Single Nucleotide; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics*; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007075:Immunoglobulin M; D012367:RNA, Viral; D014759:Viral Envelope Proteins