title: Comparison of platelet serotonin transporter activity in subjects with severe sleep bruxism and control.
abstract: OBJECTIVE
  The aim of this study was to evaluate the correlation between sleep bruxism (SB) frequency and serotonin transporter (SERT)-driven serotonin (5-HT)-uptake in platelets.
  
  
  METHODS
  Subjects were dental trainee residents and faculty members of Okayama University Hospital who were aware of having severe or no SB. SB frequency was assessed for 3-consecutive nights by a self-contained electromyographic detector/analyzer, which indicated individual SB levels as one of four grades (score 0, 1, 2 and 3). Subjects were classified as normal control (NC) when SB scores indicated only 0 or 1 during the 3 nights, or as severe SB for scores 2 or 3. Those subjects whose scores fluctuated from 0 to 3 during the 3 nights were omitted from further analysis. Fasting peripheral venous blood samples were collected in the morning following the final SB assessment. Amounts of SERTs proteins collected from peripheral platelets were quantified using ELISA, and SERTs transport activity was assessed by uptake assay using [3H]-5-HT.
  
  
  RESULTS
  Thirteen severe SB subjects and 7 NC subjects were eligible. Gender distribution, mean age, 5-HT concentration and total amounts of SERT protein in platelets showed no significant differences between NC and severe SB (p=0.85: Chi-squared test; p=0.64, 0.26, 0.46: t-test). However, [3H]-5-HT uptake by platelets was significantly greater in NC compared to severe SB subjects (12.79±1.97, 8.27±1.91 fmol/10(5) platelets/min, p<0.001, t-test).
  
  
  CONCLUSIONS
  The results of this pilot study suggest a possible correlation between peripheral platelet serotonin transporter uptake ability and SB severity.
authors.affiliation: Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Minakuchi
authors.affiliation: Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Chiharu
authors.initials: C
authors.lastname: Sogawa
authors.affiliation: Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Emilio Satoshi
authors.initials: ES
authors.lastname: Hara
authors.affiliation: Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Haruna
authors.initials: H
authors.lastname: Miki
authors.affiliation: Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Maekawa
authors.affiliation: Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Sogawa
authors.affiliation: Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Kitayama
authors.affiliation: Department of Fixed Prosthodontics, Institute of Health Biosciences Graduate School, The University of Tokushima, Tokushima, Japan.
authors.forename: Yoshizo
authors.initials: Y
authors.lastname: Matsuka
authors.affiliation: Advanced Program in Orofacial Pain and Oral Medicine, Ostrow School of Dentistry, University of Southern California, USA.
authors.forename: Glenn T
authors.initials: GT
authors.lastname: Clark
authors.affiliation: Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan. Electronic address: kuboki@md.okayama-u.ac.jp.
authors.forename: Takuo
authors.initials: T
authors.lastname: Kuboki
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000378:metabolism; D004576:Electromyography; D005260:Female; D006801:Humans; D008297:Male; D010976:Platelet Count; D059326:Serotonergic Neurons / Q000378:metabolism*; D012701:Serotonin / Q000097:blood; D050486:Serotonin Plasma Membrane Transport Proteins / Q000097:blood / Q000378:metabolism*; D012720:Severity of Illness Index; D020186:Sleep Bruxism / Q000097:blood* / Q000175:diagnosis / Q000378:metabolism*; D055815:Young Adult
chemical_list: D050486:Serotonin Plasma Membrane Transport Proteins; D012701:Serotonin
keywords: Electromyography (EMG); Human serotonin transporter (hSERT); Sleep bruxism; Uptake ability