title: Protective effects of intranasal immunization with recombinant glycoprotein d in pregnant BALB/c mice challenged with different strains of equine herpesvirus 1.
abstract: Equine herpesvirus (EHV)-1 induces respiratory infection, neurological disorders and abortion in horses. Most of the currently available attenuated or inactivated vaccines against this infection are administered intramuscularly and only provide partial protection against the respiratory disease. The present study examines the effect of intranasal immunization with purified EHV-1 recombinant glycoprotein D (gD) in BALB/c mice followed by challenge with three different EHV-1 strains during early to mid-pregnancy. The induced viral infection was evaluated by virus isolation, DNA detection by polymerase chain reaction, histopathology and immunohistochemical localization of antigen in the lung, placenta and uterus. Non-immunized mice showed clinical signs of infection, positive virus isolation from lungs and uteri, and abortion induced by one of the virus strains. Endometrial lesions developed in some of these animals that have been described previously only in horses. Immunized mice and their offspring had no viral infection or typical lesions. Intranasally administered gD therefore induced partial or complete protection against three different EHV-1 strains in BALB/c mice.
authors.affiliation: Department of Virology, School of Veterinary Sciences, National University of La Plata, Buenos Aires, Argentina; National Scientific and Technical Research Council (CONICET-CCT La Plata). Electronic address: nadiafuentealba@hotmail.com.
authors.forename: N A
authors.initials: NA
authors.lastname: Fuentealba
authors.affiliation: Department of Histology and Embryology, School of Veterinary Sciences, National University of La Plata, Buenos Aires, Argentina; Department of General Pathology, School of Veterinary Sciences, National University of La Plata, Buenos Aires, Argentina; National Scientific and Technical Research Council (CONICET-CCT La Plata).
authors.forename: C N
authors.initials: CN
authors.lastname: Zanuzzi
authors.affiliation: Department of Virology, School of Veterinary Sciences, National University of La Plata, Buenos Aires, Argentina; Department of Histology and Embryology, School of Veterinary Sciences, National University of La Plata, Buenos Aires, Argentina; National Scientific and Technical Research Council (CONICET-CCT La Plata).
authors.forename: M R
authors.initials: MR
authors.lastname: Scrochi
authors.affiliation: Department of Virology, School of Veterinary Sciences, National University of La Plata, Buenos Aires, Argentina.
authors.forename: G H
authors.initials: GH
authors.lastname: Sguazza
authors.affiliation: Department of Virology, School of Veterinary Sciences, National University of La Plata, Buenos Aires, Argentina; Argentinean Agency for the Promotion of Science and Technology (ANPCyT), Argentina.
authors.forename: M E
authors.initials: ME
authors.lastname: Bravi
authors.affiliation: Department of Virology, School of Veterinary Sciences, National University of La Plata, Buenos Aires, Argentina; Scientific Research Commission of Buenos Aires Province (CIC-PBA).
authors.forename: V
authors.initials: V
authors.lastname: Cid de la Paz
authors.affiliation: Department of Epidemiology, School of Veterinary Sciences, National University of La Plata, Buenos Aires, Argentina.
authors.forename: S G
authors.initials: SG
authors.lastname: Corva
authors.affiliation: Department of General Pathology, School of Veterinary Sciences, National University of La Plata, Buenos Aires, Argentina; National Scientific and Technical Research Council (CONICET-CCT La Plata).
authors.forename: E L
authors.initials: EL
authors.lastname: Portiansky
authors.affiliation: Department of General Pathology, School of Veterinary Sciences, National University of La Plata, Buenos Aires, Argentina; National Scientific and Technical Research Council (CONICET-CCT La Plata).
authors.forename: E J
authors.initials: EJ
authors.lastname: Gimeno
authors.affiliation: Department of Histology and Embryology, School of Veterinary Sciences, National University of La Plata, Buenos Aires, Argentina; Department of General Pathology, School of Veterinary Sciences, National University of La Plata, Buenos Aires, Argentina; National Scientific and Technical Research Council (CONICET-CCT La Plata).
authors.forename: C G
authors.initials: CG
authors.lastname: Barbeito
authors.affiliation: Department of Virology, School of Veterinary Sciences, National University of La Plata, Buenos Aires, Argentina; Argentinean Agency for the Promotion of Science and Technology (ANPCyT), Argentina.
authors.forename: C M
authors.initials: CM
authors.lastname: Galosi
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006566:Herpesviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D004861:Herpesvirus 1, Equid; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology; D014611:Vaccination / Q000379:methods*; D014759:Viral Envelope Proteins / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D011994:Recombinant Proteins; D014759:Viral Envelope Proteins
keywords: equine herpesvirus 1; glycoprotein D; intranasal inoculation; mouse abortion model