title: Nasal sensitization with ragweed pollen induces local-allergic-rhinitis-like symptoms in mice.
abstract: Recently, the concept of local allergic rhinitis (LAR) was established, namely rhinitis symptoms with local IgE production and negative serum antigen-specific IgE. However, the natural course of LAR development and the disease pathogenesis is poorly understood. This study investigated the pathophysiology of mice with allergic rhinitis that initially sensitized with ragweed pollen through the nasal route. Mice were nasally administrated ragweed pollen over consecutive days without prior systemic immunization of the allergen. Serial nasal sensitization of ragweed pollen induced an allergen-specific increase in sneezing, eosinophilic infiltration, and the production of local IgE by day 7, but serum antigen-specific IgE was not detected. Th2 cells accumulated in nose and cervical lymph nodes as early as day 3. These symptoms are characteristic of human LAR. Continual nasal exposure of ragweed pollen for 3 weeks resulted in the onset of classical AR with systemic atopy and adversely affected lung inflammation when the allergen was instilled into the lung. Fcer1a(-/-) mice were defective in sneezing but developed normal eosinophilic infiltration. Contrary, Rag2(-/-) mice were defective in both sneezing and eosinophilic infiltration, suggesting that T cells play a central role in the pathogenesis of the disease. These observations demonstrate nasal allergen sensitization to non-atopic individuals can induce LAR. Because local Th2 cell accumulation is the first sign and Th2 cells have a central role in the disease, a T-cell-based approach may aid the diagnosis and treatment of LAR.
authors.affiliation: Laboratory of Allergic Diseases, Institute for Advanced Medical Sciences, Hyogo College of Medicine, Hyogo, Japan; Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medical Science, University of Fukui, Fukui, Japan.
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Kato
authors.affiliation: Laboratory of Allergic Diseases, Institute for Advanced Medical Sciences, Hyogo College of Medicine, Hyogo, Japan.
authors.forename: Shoko
authors.initials: S
authors.lastname: Akasaki
authors.affiliation: Laboratory of Allergic Diseases, Institute for Advanced Medical Sciences, Hyogo College of Medicine, Hyogo, Japan; Department of Otolaryngology-Head and Neck Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Muto-Haenuki
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medical Science, University of Fukui, Fukui, Japan.
authors.forename: Shigeharu
authors.initials: S
authors.lastname: Fujieda
authors.affiliation: Laboratory of Allergic Diseases, Institute for Advanced Medical Sciences, Hyogo College of Medicine, Hyogo, Japan.
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Matsushita
authors.affiliation: Laboratory of Allergic Diseases, Institute for Advanced Medical Sciences, Hyogo College of Medicine, Hyogo, Japan; Department of Immunology and Medical Zoology, Hyogo College of Medicine, Hyogo, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yoshimoto
mesh_terms: D000818:Animals; D052179:Antigens, Plant / Q000276:immunology*; D004195:Disease Models, Animal; D006969:Hypersensitivity, Immediate; D007114:Immunization; D051379:Mice; D009297:Nasal Mucosa / Q000276:immunology; D010936:Plant Extracts / Q000276:immunology*; D017455:Receptors, IgE / Q000235:genetics; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology*; D012912:Sneezing / Q000235:genetics; D018418:Th2 Cells / Q000276:immunology
chemical_list: D052179:Antigens, Plant; D010936:Plant Extracts; D017455:Receptors, IgE; C433085:ragweed pollen