title: Renovascular morphological changes in a rabbit model of hydronephrosis.
abstract: Obstructive nephropathy ultimately leads to end-stage renal failure. Renovascular lesions are involved in various nephropathies, and most renal diseases have an ischemic component that underlies the resulting renal fibrosis. The aim of this study was to investigate whether morphological changes occur in the renal vasculature in hydronephrosis and the possible mechanisms involved. A model of complete unilateral ureteral obstruction (CUUO) was used. Experimental animals were divided into five groups: a normal control group (N) and groups of animals at 1st week (O1), 2nd week (O2), 4th week (O4) and 8th week (O8) after CUUO. Blood pressure was measured, renal arterial trees and glomeruli were assessed quantitatively, and renovascular three-dimensional reconstruction was performed on all groups. Glomerular ultrastructural changes were examined by transmission electron microscopy. The results showed that the systolic blood pressure was significantly increased in the obstructed groups (O1, O2, O4 and O8). Three-dimensional reconstruction showed sparse arterial trees in the O8 group, and a tortuous and sometimes ruptured glomerular basement membrane was found in the O4 and O8 groups. Furthermore, epithelial media thickness and media/lumen ratio were increased, lumen diameters were decreased, and the cross-sectional area of the media was unaltered in the segmental renal artery, interlobar artery and afferent arterioles, respectively. In conclusion, renal arterial trees and glomeruli were dramatically altered following CUUO and the changes may be partially ascribed to vascular remodeling. Elucidation of the molecular mechanisms of renovascular morphological alterations will enable the development of potential therapeutic approaches for hydronephrosis.
authors.affiliation: Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors.forename: Wan-Qiang
authors.initials: WQ
authors.lastname: Li
authors.affiliation: Department of Urology, China Three Gorges University/Yichang Central People's Hospital, Yichang, 443003, China.
authors.forename: Zi-Qiang
authors.initials: ZQ
authors.lastname: Dong
authors.affiliation: The Central Laboratory of Human Morphology, University of South China, Hengyang, 421001, China.
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Zhou
authors.affiliation: Department of Urology, China Three Gorges University/Yichang Central People's Hospital, Yichang, 443003, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Long
authors.affiliation: Department of Urology, China Three Gorges University/Yichang Central People's Hospital, Yichang, 443003, China.
authors.forename: Lu-Sheng
authors.initials: LS
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, the First College of Clinical Medical Science, China Three Gorges University/Yichang Central People's Hospital, Yichang, 443003, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Zhou
authors.affiliation: Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors.forename: Ren-Ping
authors.initials: RP
authors.lastname: Zheng
authors.affiliation: Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, China. zhangjie_8888@sina.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001794:Blood Pressure; D004195:Disease Models, Animal; D050533:Glomerular Basement Membrane / Q000098:blood supply / Q000473:pathology / Q000503:physiopathology; D006869:Hydronephrosis / Q000473:pathology / Q000503:physiopathology; D008297:Male; D011817:Rabbits; D012077:Renal Artery / Q000473:pathology / Q000503:physiopathology