title: Lectin affinity electrophoresis.
abstract: An interaction or a binding event typically changes the electrophoretic properties of a molecule. Affinity electrophoresis methods detect changes in the electrophoretic pattern of molecules (mainly macromolecules) that occur as a result of biospecific interactions or complex formation. Lectin affinity electrophoresis is a very effective method for the detection and analysis of trace amounts of glycobiological substances. It is particularly useful for isolating and separating the glycoisomers of target molecules. Here, we describe a sensitive technique for the detection of glycoproteins separated by agarose gel-lectin affinity electrophoresis that uses antibody-affinity blotting. The technique is tested using α-fetoprotein with lectin (Lens culinaris agglutinin and Phaseolus vulgaris agglutinin)-agarose gels.
authors.affiliation: J-Oil Mills Inc., 11, Kagetoricho, Totsuka-ku, Yokohama, Kanagawa, 245-0064, Japan, yuka.kobayashi@j-oil.com.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kobayashi
mesh_terms: D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D037121:Plant Lectins / Q000378:metabolism*; D012685:Sepharose / Q000737:chemistry; D000509:alpha-Fetoproteins / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D037121:Plant Lectins; D000509:alpha-Fetoproteins; D012685:Sepharose