title: Statistically quantified measurement of an Alzheimer's marker by surface-enhanced Raman scattering.
abstract: Fibrillar forms of the Amyloid-β (Aβ) protein have been implicated in the early stages of Alzheimer's disease (AD), however there are no standardised assays for soluble Aβ oligomer biomarkers that provide the best indication of the disease progression [1,2]. As a step towards a fast and label-free method for testing different AD biomarkers, we have combined laser nano-textured substrates with a SERS mapping technique and validated it using soluble Aβ-40 oligomers [3-5]. The nano-textured SERS substrates provide fast (&5 min), label-free spectra associated with soluble Aβ-40 oligomers down to a concentration of 10 nM. Statistical analysis of the spectral intensities mapped over the substrate surface shows a quantitative correlation with the oligomer concentration. Schematics of experiments: SERS mapping of Aβ-40 (left figure: measured SERS intensity overlayed with an SEM image of ripples) was carried out on the laser nano-textured (ripple) surface of sapphire and statistical analysis of the SERS intensity was carried out for qualitative (a high SERS intensity at low probability) and quantitative (a moderate SERS intenisty at the highest probability) measures. Quantitative statistical analysis of SERS mapping data can be performed off line for cross correlations with other known SERS signatures.
authors.affiliation: Centre for Micro-Photonics and The Australian National Fabrication Facility - ANFF, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.
authors.forename: Ričardas
authors.initials: R
authors.lastname: Buividas
authors.affiliation: Centre for Micro-Photonics and The Australian National Fabrication Facility - ANFF, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.|Department of Bioelectrochemistry and Biospectroscopy, Vilnius University Institute of Biochemistry, Mokslininkų 12, LT-08662 Vilnius, Lithuania.
authors.forename: Nerijus
authors.initials: N
authors.lastname: Dzingelevičius
authors.affiliation: Centre for Micro-Photonics and The Australian National Fabrication Facility - ANFF, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.|Laboratory of Biomedical Physics, Vilnius University Institute of Oncology, Baublio 3A, Vilnius, LT-08406, Lithuania.|Research Centre for Microsystems and Nanotechnology, Kaunas University of Technology, Studentu 65, LT-51369, Kaunas, Lithuania.
authors.forename: Reda
authors.initials: R
authors.lastname: Kubiliūtė
authors.affiliation: Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Stoddart
authors.affiliation: Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.
authors.forename: Vi
authors.initials: V
authors.lastname: Khanh Truong
authors.affiliation: Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.
authors.forename: Elena P
authors.initials: EP
authors.lastname: Ivanova
authors.affiliation: Centre for Micro-Photonics and The Australian National Fabrication Facility - ANFF, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.|Melbourne Centre for Nanofabrication, Australian National Fabrication Facility, Clayton, VIC 3168, Australia.
authors.forename: Saulius
authors.initials: S
authors.lastname: Juodkazis
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000737:chemistry*; D015415:Biomarkers / Q000378:metabolism; D008958:Models, Molecular; D010446:Peptide Fragments / Q000737:chemistry*; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D016229:Amyloid beta-Peptides; D015415:Biomarkers; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40)
keywords: Alzheimer's disease; SERS; amyloid‐beta; femtosecond laser fabrication; label‐free