title: An automated system using spatial oversampling for optical mapping in murine atria. Development and validation with monophasic and transmembrane action potentials.
abstract: We developed and validated a new optical mapping system for quantification of electrical activation and repolarisation in murine atria. The system makes use of a novel 2nd generation complementary metal-oxide-semiconductor (CMOS) camera with deliberate oversampling to allow both assessment of electrical activation with high spatial and temporal resolution (128 × 2048 pixels) and reliable assessment of atrial murine repolarisation using post-processing of signals. Optical recordings were taken from isolated, superfused and electrically stimulated murine left atria. The system reliably describes activation sequences, identifies areas of functional block, and allows quantification of conduction velocities and vectors. Furthermore, the system records murine atrial action potentials with comparable duration to both monophasic and transmembrane action potentials in murine atria.
authors.affiliation: Centre for Cardiovascular Sciences, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, UK; Doctoral Training Centre for Physical Sciences of Imaging in the Biomedical Sciences (PSIBS), University of Birmingham, UK.
authors.forename: Ting Yue
authors.initials: TY
authors.lastname: Yu
authors.affiliation: Centre for Cardiovascular Sciences, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, UK.
authors.forename: Fahima
authors.initials: F
authors.lastname: Syeda
authors.affiliation: Centre for Cardiovascular Sciences, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, UK.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Holmes
authors.affiliation: Centre for Cardiovascular Sciences, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, UK.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Osborne
authors.affiliation: Doctoral Training Centre for Physical Sciences of Imaging in the Biomedical Sciences (PSIBS), University of Birmingham, UK; School of Computer Science, College of Engineering and Physical Sciences, University of Birmingham, UK.
authors.forename: Hamid
authors.initials: H
authors.lastname: Dehghani
authors.affiliation: School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, UK.
authors.forename: Keith L
authors.initials: KL
authors.lastname: Brain
authors.affiliation: Centre for Cardiovascular Sciences, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, UK.
authors.forename: Paulus
authors.initials: P
authors.lastname: Kirchhof
authors.affiliation: Centre for Cardiovascular Sciences, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, UK. Electronic address: L.Fabritz@bham.ac.uk.
authors.forename: Larissa
authors.initials: L
authors.lastname: Fabritz
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D006329:Heart Conduction System / Q000502:physiology*; D051379:Mice; D009431:Neural Conduction / Q000502:physiology*; D010363:Pattern Recognition, Automated / Q000379:methods*; D010781:Photography / Q000295:instrumentation*; D015203:Reproducibility of Results; D018401:Sample Size; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation; D056969:Voltage-Sensitive Dye Imaging / Q000295:instrumentation* / Q000379:methods
keywords: Atrial action potential duration; Atrial fibrillation; Conduction velocity; Isolated atrium; Murine model; Optical mapping