title: Gene expression signatures affected by ethanol and/or nicotine in normal human normal oral keratinocytes (NHOKs).
abstract: It has been reported that nicotine/alcohol alter epigenetic control and lead to abrogated DNA methylation and histone modifications, which could subsequently perturb transcriptional regulation critically important in cellular transformation. The aim of this study is to determine the molecular mechanisms of nicotine/alcohol-induced epigenetic alterations and their mechanistic roles in transcriptional regulation in human adult stem cells. We hypothesized that nicotine/alcohol induces deregulation of epigenetic machinery and leads to epigenetic alterations, which subsequently affect transcriptional regulation in oral epithelial stem cells. As an initiating step we have profiled transcriptomic alterations induced by combinatory administration of EtOH and nicotine in primary normal human oral keratinocytes. Here we provide detailed experimental methods, analysis and information associated with our data deposited into Gene Expression Omnibus (GEO) under GSE57634. Our data provide comprehensive transcriptomic map describing molecular changes induced by EtOH and nicotine on normal human oral keratinocytes.
authors.affiliation: Laboratory of Stem Cell and Cancer Epigenetic Research, UCLA School of Dentistry, Los Angeles, CA, USA.
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Kim
authors.affiliation: Laboratory of Stem Cell and Cancer Epigenetic Research, UCLA School of Dentistry, Los Angeles, CA, USA.
authors.forename: Omar
authors.initials: O
authors.lastname: Khalid
authors.affiliation: Department of Biostatistics and Medicine, UCLA School of Public Health, Los Angeles, CA, USA.
authors.forename: Lewei
authors.initials: L
authors.lastname: Duan
authors.affiliation: Division of Oral Biology and Medicine, UCLA School of Dentistry, Los Angeles, CA, USA.
authors.forename: Reuben
authors.initials: R
authors.lastname: Kim
authors.affiliation: Department of Biostatistics and Medicine, UCLA School of Public Health, Los Angeles, CA, USA.
authors.forename: David
authors.initials: D
authors.lastname: Elashoff
authors.affiliation: Laboratory of Stem Cell and Cancer Epigenetic Research, UCLA School of Dentistry, Los Angeles, CA, USA ; Division of Oral Biology and Medicine, UCLA School of Dentistry, Los Angeles, CA, USA ; Center for Oral and Head/Neck Oncology Research Center, Los Angeles, CA, USA ; UCLA's Jonsson Comprehensive Cancer Center, Los Angeles, CA, USA ; UCLA Broad Stem Cell Research Center, Los Angeles, CA, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Kim