title: West Nile virus lineage 2 isolated from Culex modestus mosquitoes in the Czech Republic, 2013: expansion of the European WNV endemic area to the North?
abstract: We report the detection and isolation of four almost identical strains of West Nile virus (WNV) lineage 2from Culex modestus mosquitoes collected at three fish ponds in South Moravia, Czech Republic, during August 2013. Phylogenetic analysis demonstrated that the Czech WNV strains isolated are closely related to Austrian, Italian and Serbian strains reported in 2008,2011 and 2012, respectively. Our findings show the current northernmost range of lineage 2 WNV in Europe.
authors.affiliation: Institute of Vertebrate Biology, Academy of Sciences, v.v.i., Brno, Czech Republic.
authors.forename: I
authors.initials: I
authors.lastname: Rudolf
authors.forename: T
authors.initials: T
authors.lastname: Bakonyi
authors.forename: O
authors.initials: O
authors.lastname: Sebesta
authors.forename: J
authors.initials: J
authors.lastname: Mendel
authors.forename: J
authors.initials: J
authors.lastname: Peško
authors.forename: L
authors.initials: L
authors.lastname: Betášová
authors.forename: H
authors.initials: H
authors.lastname: Blažejová
authors.forename: K
authors.initials: K
authors.lastname: Venclíková
authors.forename: P
authors.initials: P
authors.lastname: Straková
authors.forename: N
authors.initials: N
authors.lastname: Nowotny
authors.forename: Z
authors.initials: Z
authors.lastname: Hubálek
mesh_terms: D000818:Animals; D003465:Culex / Q000821:virology*; D009033:Culicidae / Q000821:virology; D018153:Czech Republic / Q000453:epidemiology; D019353:Endemic Diseases; D005060:Europe / Q000453:epidemiology; D007303:Insect Vectors / Q000821:virology; D007558:Italy; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D014901:West Nile Fever / Q000453:epidemiology; D014902:West Nile virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D012367:RNA, Viral