title: A mitogen-activated protein kinase cascade module, MKK3-MPK6 and MYC2, is involved in blue light-mediated seedling development in Arabidopsis.
abstract: Mitogen-activated protein kinase (MAPK) pathways are involved in several signal transduction processes in eukaryotes. Light signal transduction pathways have been extensively studied in plants; however, the connection between MAPK and light signaling pathways is currently unknown. Here, we show that MKK3-MPK6 is activated by blue light in a MYC2-dependent manner. MPK6 physically interacts with and phosphorylates a basic helix-loop-helix transcription factor, MYC2, and is phosphorylated by a MAPK kinase, MKK3. Furthermore, MYC2 binds to the MPK6 promoter and regulates its expression in a feedback regulatory mechanism in blue light signaling. We present mutational and physiological studies that illustrate the function of the MKK3-MPK6-MYC2 module in Arabidopsis thaliana seedling development and provide a revised mechanistic view of photomorphogenesis.
authors.affiliation: National Institute of Plant Genome Research, New Delhi 110067, India.
authors.forename: Vishmita
authors.initials: V
authors.lastname: Sethi
authors.affiliation: National Institute of Plant Genome Research, New Delhi 110067, India.
authors.forename: Badmi
authors.initials: B
authors.lastname: Raghuram
authors.affiliation: National Institute of Plant Genome Research, New Delhi 110067, India alok@nipgr.ac.in.
authors.forename: Alok Krishna
authors.initials: AK
authors.lastname: Sinha
authors.affiliation: National Institute of Plant Genome Research, New Delhi 110067, India Department of Biotechnology, National Institute of Technology, Durgapur 713209, India.
authors.forename: Sudip
authors.initials: S
authors.lastname: Chattopadhyay
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D008027:Light; D048371:MAP Kinase Kinase 3 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020935:MAP Kinase Signaling System / Q000502:physiology*; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010766:Phosphorylation; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects
chemical_list: D029681:Arabidopsis Proteins; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; C473611:MYC2 protein, Arabidopsis; C436293:MPK6 protein, Arabidopsis; D020928:Mitogen-Activated Protein Kinases; D048371:MAP Kinase Kinase 3