title: Cyclin D1b overexpression inhibits cell proliferation and induces cell apoptosis in cervical cancer cells in vitro and in vivo.
abstract: Cyclin D1b is one of two proteins translated from cyclin D1 transcripts (isoforms a and b) that are generated due to gene polymorphism. Our previous study has reported low cyclin D1b expression in cervical cancer tissue, with an expression level in moderately or poorly differentiated tissues that was significantly lower than that in well-differentiated tissues. However, the functional role of cyclin D1b in cervical cancer remains to be elucidated. In this study, using a cervical cancer cell line with stable expression of cyclin D1b, we found that upregulation of cyclin D1b initiated cell cycle arrest at the G0/G1 phase and induced apoptosis, thereby inhibiting cell proliferation and colony formation. Furthermore, xenograft transplantation experiments in nude mice demonstrated that cyclin D1b upregulation inhibited cancer growth and induce apoptosis in vivo. In conclusion, the present study indicates anti-tumor effects of cyclin D1b in cervical cancer, suggesting that cyclin D1b may represent a potential therapeutic target for cervical cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University Shenyang 110004, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University Shenyang 110004, People's Republic of China.
authors.forename: Heng
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University Shenyang 110004, People's Republic of China.
authors.forename: Duo
authors.initials: D
authors.lastname: Yin
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University Shenyang 110004, People's Republic of China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University Shenyang 110004, People's Republic of China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University Shenyang 110004, People's Republic of China.
authors.forename: Di
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University Shenyang 110004, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University Shenyang 110004, People's Republic of China.
authors.forename: Shulan
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D049109:Cell Proliferation / Q000502:physiology; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006367:HeLa Cells; D064593:Heterografts; D006801:Humans; D020287:In Situ Nick-End Labeling; D066298:In Vitro Techniques; D051379:Mice; D008819:Mice, Nude; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*
chemical_list: D020033:Protein Isoforms; D019938:Cyclin D1
keywords: Cyclin D1b; apoptosis; cervical cancer; proliferation