title: Simultaneous determination of seven bufadienolides in rat plasma after oral administration of Shexiang Baoxin Pill by liquid chromatography-electrospray ionization-tandem mass spectrometry: application to a pharmacokinetic study.
abstract: A liquid chromatography-electrospray ionization-tandem mass spectrometry method was described for the simultaneous determination of resibufogenin, bufalin, gamabufotalin, telibufagin, arenobufagin, cinobufagin and bufotalin in rat plasma. Plasma samples were pretreated by liquid-liquid extraction with ethyl acetate. Chromatographic separation was carried out on an ACQUITY HSS T3 column with gradient elution using mobile phase consisting of acetonitrile-0.1% formic acid in water at a flow rate of 0.3 mL/min. All analytes showed good linearity over a wide concentration range (r>0.99). The lower limit of quantification was in the range of 0.5-10 ng/mL for seven bufadienolides. The mean recovery of the analytes ranged from 94.36 to 104.18%. The intra- and inter-day precisions were in the range of 1.74-13.78% and the accuracies were between 89.37 and 101.38%. The validated method was successfully applied to a pharmacokinetic (PK) study of the seven bufadienolides in rat plasma after oral administration of Shexiang Baoxin Pill (SBP). The selected PK marker compounds with typical efficacy/toxicity may provide a practical solution for marker compound selection and dosage design for the therapeutic drug monitoring and PK study of SBP in its clinical applications.
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China; Department of Pharmacy, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200001, PR China.
authors.forename: Shuping
authors.initials: S
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Peng
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Fu
authors.affiliation: Pharmacy Department, Shanghai Yangsi Hosipital, Shanghai 200126, PR China.
authors.forename: Jianfei
authors.initials: J
authors.lastname: Tao
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Han
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China.
authors.forename: Huimei
authors.initials: H
authors.lastname: Huang
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China.
authors.forename: Wanlin
authors.initials: W
authors.lastname: Chang
authors.affiliation: School of Chemistry and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, PR China.
authors.forename: Le
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China; School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China. Electronic address: wdzhangy@hotmail.com.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China. Electronic address: lyliurh@126.com.
authors.forename: Runhui
authors.initials: R
authors.lastname: Liu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002018:Bufanolides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002018:Bufanolides; D004365:Drugs, Chinese Herbal; C526712:shexiang baoxin
keywords: Bufadienolides; LC–ESI–MS/MS; Pharmacokinetic study; Shexiang Baoxin Pill