title: Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites.
abstract: Hydroxyapatite (HA) is considered to be a bioactive material that favorably influences the adhesion, growth, and osteogenic differentiation of osteoblasts. To optimize the cell response on the hydroxyapatite composite, it is desirable to assess the optimum concentration and also the optimum particle size. The aim of our study was to prepare composite materials made of polydimethylsiloxane, polyamide, and nano-sized (N) or micro-sized (M) HA, with an HA content of 0%, 2%, 5%, 10%, 15%, 20%, 25% (v/v) (referred to as N0-N25 or M0-M25), and to evaluate them in vitro in cultures with human osteoblast-like MG-63 cells. For clinical applications, fast osseointegration of the implant into the bone is essential. We observed the greatest initial cell adhesion on composites M10 and N5. Nano-sized HA supported cell growth, especially during the first 3 days of culture. On composites with micro-size HA (2%-15%), MG-63 cells reached the highest densities on day 7. Samples M20 and M25, however, were toxic for MG-63 cells, although these composites supported the production of osteocalcin in these cells. On N2, a higher concentration of osteopontin was found in MG-63 cells. For biomedical applications, the concentration range of 5%-15% (v/v) nano-size or micro-size HA seems to be optimum.
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology, Prague, Czech Republic.
authors.forename: Elena
authors.initials: E
authors.lastname: Filová
authors.affiliation: Department of Composite and Carbon Materials, Institute of Rock Structure and Mechanics, Academy of Sciences of the Czech Republic, Prague, Czech Republic ; Laboratory of Biomechanics, Department of Mechanics, Biomechanics and Mechatronics, Faculty of Mechanical Engineering, CTU in Prague, Prague, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Suchý
authors.affiliation: Department of Composite and Carbon Materials, Institute of Rock Structure and Mechanics, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Zbyněk
authors.initials: Z
authors.lastname: Sucharda
authors.affiliation: Department of Composite and Carbon Materials, Institute of Rock Structure and Mechanics, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Monika
authors.initials: M
authors.lastname: Supová
authors.affiliation: Department of Composite and Carbon Materials, Institute of Rock Structure and Mechanics, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Margit
authors.initials: M
authors.lastname: Zaloudková
authors.affiliation: Department of Composite and Carbon Materials, Institute of Rock Structure and Mechanics, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Balík
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology, Prague, Czech Republic.
authors.forename: Věra
authors.initials: V
authors.lastname: Lisá
authors.affiliation: Department of Morphology and Rheology of Polymer Materials, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Slouf
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology, Prague, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Bačáková
mesh_terms: D045744:Cell Line, Tumor; D002468:Cell Physiological Phenomena / Q000187:drug effects*; D003598:Cytoskeletal Proteins / Q000378:metabolism; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D008863:Microspheres; D053758:Nanoparticles / Q000737:chemistry*; D010006:Osteoblasts; D015675:Osteocalcin / Q000378:metabolism; D053495:Osteopontin / Q000378:metabolism; D010316:Particle Size; D012995:Solubility; D013052:Spectrometry, X-Ray Emission
chemical_list: D003598:Cytoskeletal Proteins; D015675:Osteocalcin; D053495:Osteopontin; D017886:Durapatite
keywords: composite materials; hydroxyapatite; nanoparticles; osteoblasts