title: Chemical constituents of Morus alba L. and their inhibitory effect on 3T3-L1 preadipocyte proliferation and differentiation.
abstract: Mulberry leaf, an important traditional Chinese medicine, possesses many biological activities, including effects of anti-obesity. However, which constituents of mulberry leaf are responsible for its anti-adipogenic action is unclear. This study primarily investigated the chemical constituents from mulberry leaf and their bioactivity on the proliferation and differentiation of 3T3-L1 preadipocytes. A new flavane derivative, (2S)-4'-hydroxy-7-methoxy-8-prenylflavan (1), together with twelve known compounds including three flavanes (2-4), three chalcones (5-7), two flavones (8-9), two benzofurans (10-11) and two coumarin (12-13) was isolated from mulberry leaf. The structure of the new compound was elucidated by various spectroscopic methods including UV, HR-ESI-MS, (1)H and (13)C NMR and CD. The results of activity screening showed that compound 2, 6 and 7 inhibited the proliferation and differentiation of 3T3-L1 preadipocytes.
authors.affiliation: Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha 410078, China; Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Central South University, Changsha 410013, China.
authors.forename: Yongyu
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Central South University, Changsha 410013, China.
authors.forename: Xiding
authors.initials: X
authors.lastname: Yang
authors.affiliation: Sanjiu Medical & Pharmaceutical Co., Ltd, Shenzhen 518029, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Central South University, Changsha 410013, China.
authors.forename: Guangyao
authors.initials: G
authors.lastname: Zeng
authors.affiliation: Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Central South University, Changsha 410013, China.
authors.forename: Jianbing
authors.initials: J
authors.lastname: Tan
authors.affiliation: Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Central South University, Changsha 410013, China.
authors.forename: Xi
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha 410078, China. Electronic address: huchangping@yahoo.com.
authors.forename: Changping
authors.initials: C
authors.lastname: Hu
authors.affiliation: Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Central South University, Changsha 410013, China. Electronic address: zhouyingjun@csu.edu.cn.
authors.forename: YingJun
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000737:chemistry*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D031625:Morus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry
keywords: (2S)-4′-hydroxy-7-methoxy-8-prenylflavan; (2S)-4′-hydroxy-7-methoxy-8-prenylflavan (PubChem CID: No CID); 2′,4′-Dihydroxy-7′-methoxy-8-prenylflavan (PubChem CID: No CID); 2′,7-Dihydroxy-4′-methoxy-8-prenylflavan (PubChem CID: No CID); 3T3-L1 preadipocyte; 7-Hydroxy-6-methoxycoumarin (PubChem CID: 5280460); 7-Hydroxycoumarin (PubChem CID: 5281426); Astragalin (PubChem CID: 5282102); Brosimine B (PubChem CID: No CID); Flavane derivative; Isobavachalcone (PubChem CID: 5281255); Isoquercitrin (PubChem CID: 5280804); Morachalcone A (PubChem CID: 9862769); Morachalcone B (PubChem CID: No CID); Moracin C (PubChem CID: 155248); Moracin M (PubChem CID: 185848); Mulberry leaf; Obesity