title: Neuroprotective peptide-macrocycle conjugates reveal complex structure-activity relationships in their interactions with amyloid β.
abstract: Interactions between amyloid β (Aβ) and metal ions are thought to mediate the neuropathogenic effects of Aβ in Alzheimer's disease. The construction of small molecules capable of synergistically chelating metal ions and recognizing Aβ would allow new insights into the biology of this disease and provide a possible therapeutic approach. We report herein the synthesis and biological evaluation of tetraazamacrocycle-(G)KLVFF hybrids and their metal complexes. The results obtained from ThT and bis-ANS extrinsic fluorescence assays, tyrosine intrinsic fluorescence assay and proteolytic assay imply complex, multifaceted structure-activity relationships in the interaction of these conjugates with Aβ. Many of the compounds tested rescued cells from Aβ-induced cytotoxicity. The attendant simplicity and ready diversification of the synthesis of these conjugates makes them attractive for further investigation.
authors.affiliation: School of Chemistry, The University of Sydney, Sydney, New South Wales 2006, Australia. matthew.todd@sydney.edu.au peter.rutledge@sydney.edu.au.
authors.forename: Mingfeng
authors.initials: M
authors.lastname: Yu
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Ryan
authors.forename: Samantha
authors.initials: S
authors.lastname: Ellis
authors.forename: Ashley I
authors.initials: AI
authors.lastname: Bush
authors.forename: James A
authors.initials: JA
authors.lastname: Triccas
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Rutledge
authors.forename: Matthew H
authors.initials: MH
authors.lastname: Todd
mesh_terms: D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides; D002460:Cell Line; D002614:Chelating Agents / Q000737:chemistry / Q000494:pharmacology*; D056831:Coordination Complexes / Q000737:chemistry / Q000494:pharmacology; D015195:Drug Design; D006801:Humans; D047028:Macrocyclic Compounds / Q000737:chemistry / Q000494:pharmacology*; D008670:Metals / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D010446:Peptide Fragments
chemical_list: D016229:Amyloid beta-Peptides; D002614:Chelating Agents; D056831:Coordination Complexes; D047028:Macrocyclic Compounds; D008670:Metals; D018696:Neuroprotective Agents; D009842:Oligopeptides; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42)