title: Gallic acid regulates skin photoaging in UVB-exposed fibroblast and hairless mice.
abstract: Ultraviolet (UV) radiation is the primary factor in skin photoaging, which is characterized by wrinkle formation, dryness, and thickening. The mechanisms underlying skin photoaging are closely associated with degradation of collagen via upregulation of matrix metalloproteinase (MMP) activity, which is induced by reactive oxygen species (ROS) production. Gallic acid (GA), a phenolic compound, possesses a variety of biological activities including antioxidant and antiinflammatory activities. We investigated the protective effects of GA against photoaging caused by UVB irradiation using normal human dermal fibroblasts (NHDFs) in vitro and hairless mice in vivo. The production levels of ROS, interlukin-6, and MMP-1 were significantly suppressed, and type I procollagen expression was stimulated in UVB-irradiated and GA-treated NHDFs. GA treatment inhibited the activity of transcription factor activation protein 1. The effects of GA following topical application and dietary administration were examined by measuring wrinkle formation, histological modification, protein expression, and physiological changes such as stratum corneum hydration, transepidermal water loss, and erythema index. We found that GA decreased dryness, skin thickness, and wrinkle formation via negative modulation of MMP-1 secretion and positive regulation of elastin, type I procollagen, and transforming growth factor-β1. Our data indicate that GA is a potential candidate for the prevention of UVB-induced premature skin aging.
authors.affiliation: Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University, Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Korea.
authors.forename: Eunson
authors.initials: E
authors.lastname: Hwang
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Park
authors.forename: Hyun Ji
authors.initials: HJ
authors.lastname: Lee
authors.forename: Tae Youp
authors.initials: TY
authors.lastname: Lee
authors.forename: Zheng-Wang
authors.initials: ZW
authors.lastname: Sun
authors.forename: Tae Hoo
authors.initials: TH
authors.lastname: Yi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000528:radiation effects; D005707:Gallic Acid / Q000494:pharmacology*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D051379:Mice; D008812:Mice, Hairless; D017382:Reactive Oxygen Species / Q000378:metabolism; D012867:Skin / Q000187:drug effects / Q000528:radiation effects; D015595:Skin Aging / Q000187:drug effects* / Q000528:radiation effects; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D024042:Collagen Type I; D015850:Interleukin-6; D017382:Reactive Oxygen Species; D053773:Transforming Growth Factor beta1; D005707:Gallic Acid; D020781:Matrix Metalloproteinase 1
keywords: MMP-1; ROS; gallic acid; hairless mice; photoaging; type I procollagen