title: Targeted high-throughput sequencing identifies pathogenic mutations in KCNQ4 in two large Chinese families with autosomal dominant hearing loss.
abstract: Autosomal dominant non-syndromic hearing loss (ADNSHL) is highly heterogeneous, among them, KCNQ4 is one of the most frequent disease-causing genes. More than twenty KCNQ4 mutations have been reported, but none of them were detected in Chinese mainland families. In this study, we identified a novel KCNQ4 mutation in a five generation Chinese family with 84 members and a known KCNQ4 mutation in a six generation Chinese family with 66 members. Mutation screening of 30 genes for ADNSHL was performed in the probands from thirty large Chinese families with ADNSHL by targeted region capture and high-throughput sequencing. The candidate variants and the co-segregation of the phenotype were verified by polymerase chain reaction (PCR) amplification and Sanger sequencing in all ascertained family members. Then we identified a novel KCNQ4 mutation p.W275R in exon 5 and a known KCNQ4 mutation p.G285S in exon 6 in two large Chinese ADNSHL families segregating with post-lingual high frequency-involved and progressive sensorineural hearing loss. This is the first report of KCNQ4 mutation in Chinese mainland families. KCNQ4, a member of voltage-gated potassium channel family, is likely to be a common gene in Chinese patients with ADNSHL. The results also support that the combination of targeted enrichment and high-throughput sequencing is a valuable molecular diagnostic tool for autosomal dominant hereditary deafness.
authors.affiliation: Institute of Otolaryngology, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.
authors.forename: Hongyang
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institute of Otolaryngology, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: BGI-Tianjin, Tianjin, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Yi
authors.affiliation: Institute of Otolaryngology, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Institute of Otolaryngology, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Institute of Otolaryngology, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Institute of Otolaryngology, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Institute of Otolaryngology, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Lan
authors.affiliation: Institute of Otolaryngology, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Institute of Otolaryngology, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Guan
authors.affiliation: Institute of Otolaryngology, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.
authors.forename: Zifang
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Institute of Otolaryngology, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.affiliation: Institute of Otolaryngology, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.
authors.forename: Feifan
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Institute of Otolaryngology, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zong
authors.affiliation: Institute of Otolaryngology, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.
authors.forename: Wenping
authors.initials: W
authors.lastname: Xiong
authors.affiliation: Institute of Otolaryngology, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Yu
authors.affiliation: BGI-Tianjin, Tianjin, China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Song
authors.affiliation: BGI-Tianjin, Tianjin, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yi
authors.affiliation: BGI-Tianjin, Tianjin, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Yang
authors.affiliation: Unité de Génétique et Physiologie de l'Audition, Institut Pasteur, Paris, France; UMRS 1120, Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France; Université Pierre et Marie Curie (Paris VI), Paris, France; Collège de France, Paris, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Petit
authors.affiliation: Institute of Otolaryngology, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.
authors.forename: Qiuju
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D017668:Age of Onset; D000368:Aged; D000595:Amino Acid Sequence; D001299:Audiometry; D002648:Child; D002675:Child, Preschool; D002681:China; D004252:DNA Mutational Analysis; D005260:Female; D023061:Gene Order; D005799:Genes, Dominant; D005838:Genotype; D006319:Hearing Loss, Sensorineural / Q000175:diagnosis / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D051656:KCNQ Potassium Channels / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D010375:Pedigree; D016415:Sequence Alignment; D055815:Young Adult
chemical_list: D051656:KCNQ Potassium Channels; C117367:KCNQ4 protein, human