title: Solution and crystal structure of BA42, a protein from the Antarctic bacterium Bizionia argentinensis comprised of a stand-alone TPM domain.
abstract: The structure of the BA42 protein belonging to the Antarctic flavobacterium Bizionia argentinensis was determined by nuclear magnetic resonance and X-ray crystallography. This is the first structure of a member of the PF04536 family comprised of a stand-alone TPM domain. The structure reveals a new topological variant of the four β-strands constituting the central β-sheet of the αβα architecture and a double metal binding site stabilizing a pair of crossing loops, not observed in previous structures of proteins belonging to this family. BA42 shows differences in structure and dynamics in the presence or absence of bound metals. The affinity for divalent metal ions is close to that observed in proteins that modulate their activity as a function of metal concentration, anticipating a possible role for BA42.
authors.affiliation: Fundación Instituto Leloir, IIBBA-CONICET, Patricias Argentinas 435 (C1405BWE), Buenos Aires, Argentina.
authors.forename: Martin
authors.initials: M
authors.lastname: Aran
authors.forename: Clara
authors.initials: C
authors.lastname: Smal
authors.forename: Leonardo
authors.initials: L
authors.lastname: Pellizza
authors.forename: Mariana
authors.initials: M
authors.lastname: Gallo
authors.forename: Lisandro H
authors.initials: LH
authors.lastname: Otero
authors.forename: Sebastián
authors.initials: S
authors.lastname: Klinke
authors.forename: Fernando A
authors.initials: FA
authors.lastname: Goldbaum
authors.forename: Esteban R
authors.initials: ER
authors.lastname: Ithurralde
authors.forename: Andrés
authors.initials: A
authors.lastname: Bercovich
authors.forename: Walter P
authors.initials: WP
authors.lastname: Mac Cormack
authors.forename: Adrián G
authors.initials: AG
authors.lastname: Turjanski
authors.forename: Daniel O
authors.initials: DO
authors.lastname: Cicero
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000864:Antarctic Regions; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D002942:Circular Dichroism; D018360:Crystallography, X-Ray; D041965:Flavobacteriaceae / Q000737:chemistry*; D008670:Metals / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D055550:Protein Stability; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D008670:Metals; D002118:Calcium
keywords: Antarctic bacteria; BA42; Bizionia argentinensis; X-ray crystallography; nuclear magnetic resonance; protein structure; structural genomics