title: [The effect of forsythiaside on the expression of c-jun induced by cisplatin in the cochlea of guinea pig].
abstract: OBJECTIVE
  To study the effect of forsythiaside on the expression of c-jun induced by cisplatin in the cochlea of guinea pig.
  
  
  METHODS
  Thirty guinea pigs were randomly divided into control group (10), cisplatin group (10) and forsythiaside group (10). The ototoxicity model was done with intraperitoneal injection of cisplatin solution (8 mg/kg per day) for 7 days. Forsythiaside (25 mg/kg per day) was injected 30 min before cisplatin solution treated in guinea pigs of forsythiaside group for 7 consecutive days. The saline instead of cisplatin was injected in normal control group. The distortion product otoacoustic emission (DPOAE) was detected before animals were killed. The expression of c-jun in cochlea of guinea pigs was detected by western blotting. The expression of c-jun mRNA in cochlea of guinea pigs was detected by reverse transcriptase polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  DPOAE amplitudes in cisplatin group was significantly lower than in control group (P < 0.01). Compared with cisplatin group, DPOAE amplitudes in forsythiaside group was increased significantly (P < 0.05). The expression of c-jun protein and mRNA were significantly increased in cisplatin group than in control group (P < 0.01). Compared with cisplatin group, the expression of c-jun protein and mRNA were significantly decreased in forsythiaside group.
  
  
  CONCLUSIONS
  Forsythiaside can significantly reduce the side effects induced by cisplatin through down-regulating the expression of c-jun.
authors.forename: Ning
authors.initials: N
authors.lastname: An
authors.forename: Shengwu
authors.initials: S
authors.lastname: Shi
mesh_terms: D000818:Animals; D002945:Cisplatin / Q000633:toxicity*; D003051:Cochlea / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006027:Glycosides / Q000494:pharmacology*; D006168:Guinea Pigs; D008297:Male; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*
chemical_list: D006027:Glycosides; D016755:Proto-Oncogene Proteins c-jun; C058049:forsythiaside; D002945:Cisplatin