title: Increased oxidative DNA damage and decreased expression of base excision repair proteins in airway epithelial cells of women who cook with biomass fuels.
abstract: To investigate whether biomass burning causes oxidative DNA damage and alters the expression of DNA base excision repair (BER) proteins in airway cells, sputum samples were collected from 80 premenopausal rural biomass-users and 70 age-matched control women who cooked with liquefied petroleum gas. Compared with control the airway cells of biomass-users showed increased DNA damage in alkaline comet assay. Biomass-users showed higher percentage of cells expressing oxidative DNA damage marker 8-oxoguanine and lower percentages of BER proteins OGG1 and APE1 by immunocytochemical staining. Reactive oxygen species (ROS) generation was doubled and level of superoxide dismutase was depleted significantly among biomass-users. The concentrations of particulate matters were higher in biomass-using households which positively correlated with ROS generation and negatively with BER proteins expressions. ROS generation was positively correlated with 8-oxoguanine and negatively with BER proteins suggesting cooking with biomass is a risk for genotoxicity among rural women in their child-bearing age.
authors.affiliation: Department of Experimental Hematology, Chittaranjan National Cancer Institute, Kolkata 700 026, India.
authors.forename: Bidisha
authors.initials: B
authors.lastname: Mukherjee
authors.affiliation: Department of Experimental Hematology, Chittaranjan National Cancer Institute, Kolkata 700 026, India.
authors.forename: Banani
authors.initials: B
authors.lastname: Bindhani
authors.affiliation: Department of Experimental Hematology, Chittaranjan National Cancer Institute, Kolkata 700 026, India.
authors.forename: Hirak
authors.initials: H
authors.lastname: Saha
authors.affiliation: Department of Experimental Hematology, Chittaranjan National Cancer Institute, Kolkata 700 026, India. Electronic address: manasrray@rediffmail.com.
authors.forename: Manas Ranjan
authors.initials: MR
authors.lastname: Ray
mesh_terms: D000328:Adult; D016902:Air Pollution, Indoor / Q000009:adverse effects / Q000032:analysis; D018533:Biomass; D003296:Cooking; D004249:DNA Damage; D045647:DNA Glycosylases / Q000378:metabolism; D004260:DNA Repair; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005579:Fossil Fuels / Q000009:adverse effects* / Q000032:analysis; D005786:Gene Expression Regulation / Q000187:drug effects*; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism; D006801:Humans; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D012906:Smoke / Q000009:adverse effects* / Q000032:analysis; D013183:Sputum / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D055815:Young Adult
chemical_list: D005579:Fossil Fuels; D017382:Reactive Oxygen Species; D012906:Smoke; C024829:8-hydroxyguanine; D006147:Guanine; D013482:Superoxide Dismutase; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human; C085108:APEX1 protein, human; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase
keywords: Airway cells; Base excision repair; Biomass fuel; Oxidative DNA damage; Oxidative stress; Particulate matter