title: Detection of antibodies to the biofilm exopolysaccharide of Histophilus somni following infection in cattle by enzyme-linked immunosorbent assay.
abstract: An enzyme-linked immunosorbent assay (ELISA) was developed to detect bovine antibodies to Histophilus somni exopolysaccharide (EPS), which is created during biofilm formation. When an index value of 0.268 was used, the sensitivity of the assay for infected calves was 90.5% at 3 weeks postinfection, but the number of positive animals increased by week 4. The specificity of the assay for healthy calves was 92.5%. The EPS ELISA may aid in identifying calves with H. somni diseases.
authors.affiliation: Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Department of Biomedical Sciences and Pathobiology, Blacksburg, Virginia, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Department of Biomedical Sciences and Pathobiology, Blacksburg, Virginia, USA.
authors.forename: Taylor
authors.initials: T
authors.lastname: Fisher
authors.affiliation: Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Department of Biomedical Sciences and Pathobiology, Blacksburg, Virginia, USA.
authors.forename: Christina
authors.initials: C
authors.lastname: Olk
authors.affiliation: Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Department of Biomedical Sciences and Pathobiology, Blacksburg, Virginia, USA Virginia Tech Carilion School of Medicine, Department of Basic Sciences, Roanoke, Virginia, USA tinzana@vt.edu.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Inzana
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology* / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015661:Pasteurellaceae / Q000276:immunology*; D016871:Pasteurellaceae Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D014730:Veterinary Medicine / Q000379:methods*
chemical_list: D000907:Antibodies, Bacterial; D011135:Polysaccharides, Bacterial