title: Physiological and biochemical changes in Brassica juncea plants under Cd-induced stress.
abstract: Plants of Brassica juncea L. var. RLC-1 were exposed for 30 days to different concentrations (0, 0.2, 0.4, and 0.6 mM) of cadmium (Cd) to analyze the Cd uptake, H2O2 content, hormonal profiling, level of photosynthetic pigments (chlorophyll, carotenoid, and flavonoid), gaseous exchange parameters (photosynthetic rate, vapour pressure deficit, intercellular CO2 concentration, and intrinsic mesophyll rate), antioxidative enzymes (superoxide dismutase, polyphenol oxidase, glutathione-S transferase, and glutathione peroxidase), antioxidant assays (DPPH, ABTS, and total phenolic content), and polyphenols. Results of the present study revealed the increased H2O2 content and Cd uptake with increasing metal doses. UPLC analysis of plants showed the presence of various polyphenols. Gaseous exchange measurements were done by infrared gas analyzer (IRGA), which was negatively affected by metal treatment. In addition, LC/MS study showed the variation in the expression of plant hormones. Level of photosynthetic pigments and activities of antioxidative enzymes were altered significantly in response to metal treatment. In conclusion, the antioxidative defence system of plants got activated due to heavy metal stress, which protects the plants by scavenging free radicals.
authors.affiliation: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005, India.
authors.forename: Dhriti
authors.identifier: 0000-0001-7523-5654
authors.initials: D
authors.lastname: Kapoor
authors.affiliation: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005, India.
authors.forename: Satwinderjeet
authors.initials: S
authors.lastname: Kaur
authors.affiliation: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005, India.
authors.forename: Renu
authors.initials: R
authors.lastname: Bhardwaj
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity*; D002245:Carbon Dioxide / Q000378:metabolism; D002853:Chromatography, Liquid; D016166:Free Radical Scavengers / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D013058:Mass Spectrometry; D009149:Mustard Plant / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology*; D010788:Photosynthesis / Q000187:drug effects; D010860:Pigments, Biological / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism; D059808:Polyphenols / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects*; D055548:Vapor Pressure
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010860:Pigments, Biological; D010937:Plant Growth Regulators; D059808:Polyphenols; D002104:Cadmium; D002245:Carbon Dioxide; D006861:Hydrogen Peroxide