title: Differentiation of meat from European wild boars and domestic pigs using polymorphisms in the MC1R and NR6A1 genes.
abstract: Wild boar meat cannot be easily distinguished from domestic pig meat, especially in processed products, thus it can be fraudulently substituted with cheaper domestic pork. In this study we genotyped polymorphisms in two genes (MC1R, affecting coat color and NR6A1, associated with number of vertebrae) in 293 domestic pigs of five commercial breeds, 111 wild boars sampled in Italy, and 90 in Slovenia and other Western Balkan regions. Allele and genotype frequency data were used to set up a DNA-based method to distinguish meat of wild boars and domestic pigs. Genotyping results indicated that domesticated genes were introgressed into wild boar populations. This complicated the determination of the origin of the meat and would cause a high error rate if markers of only one gene were used. The combined use of polymorphisms in the two analyzed genes substantially reduced false negative results.
authors.affiliation: Department of Agricultural and Food Sciences, Division of Animal Sciences, University of Bologna, Viale Fanin 46, 40127 Bologna, Italy. Electronic address: luca.fontanesi@unibo.it.
authors.forename: L
authors.initials: L
authors.lastname: Fontanesi
authors.affiliation: Department of Agricultural and Food Sciences, Division of Animal Sciences, University of Bologna, Viale Fanin 46, 40127 Bologna, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Ribani
authors.affiliation: Department of Agricultural and Food Sciences, Division of Animal Sciences, University of Bologna, Viale Fanin 46, 40127 Bologna, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Scotti
authors.affiliation: Department of Agricultural and Food Sciences, Division of Animal Sciences, University of Bologna, Viale Fanin 46, 40127 Bologna, Italy.
authors.forename: V J
authors.initials: VJ
authors.lastname: Utzeri
authors.affiliation: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 2, 21000 Novi Sad, Serbia.
authors.forename: N
authors.initials: N
authors.lastname: Veličković
authors.affiliation: Department of Agricultural and Food Sciences, Division of Animal Sciences, University of Bologna, Viale Fanin 46, 40127 Bologna, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Dall'Olio
mesh_terms: D000818:Animals; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D008297:Male; D008460:Meat; D057136:Nuclear Receptor Subfamily 6, Group A, Member 1 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D044102:Receptor, Melanocortin, Type 1 / Q000235:genetics*; D017422:Sequence Analysis, DNA; D034421:Sus scrofa; D013552:Swine
chemical_list: D005819:Genetic Markers; D057136:Nuclear Receptor Subfamily 6, Group A, Member 1; D044102:Receptor, Melanocortin, Type 1
keywords: Authentication; Coat color; Pork; SNP; Sus scrofa; Vertebral number