title: Expression of STAT5, COX-2 and PIAS3 in correlation with NSCLC histhopathological features.
abstract: Signal transducers and activators of transcription (STATs), their inhibitors and cyclooxygenase-2 (COX-2) participate in transformations of many various types of cancers. The aim of the present study was to evaluate the relationship between STAT5A/B, COX-2, and PIAS3 mRNA expression and tumor staging, metastasis status, and histopathological subtype in 71 patients with confirmed non-small cell lung cancer (NSCLC) diagnosis. Total RNA was isolated from NSCLC tissue samples and the expression of the studied genes was assessed using TaqMan probes in real-time PCR assay. The expression levels of STAT5A, STAT5B, and COX-2 genes were increased in 69%, 79%, and 71% NSCLC samples respectively, while PIAS3 expression was decreased in the majority (69%) of the studied tissues. Statistically significant differences were observed between STAT5 isoforms (P = 0.0008), with higher expression of STAT5B. We found statistically significant positive correlation between STAT5B and COX-2 (rho = 0.045), and significant negative correlation between STAT5B and PIAS3 (rho = -0.049). The negative correlation between STAT5B and PIAS3 (rho = -0.43) was also observed in T2a+T2b tumor group. Additionally, STAT5B and COX-2 expression levels were significantly different between T1a+T1b and T2a+T2b tumors (P = 0.002 and P = 0.041, respectively), with higher expression of both genes in T2 tumor stage. PIAS3 expression was significantly lower in NSCC subtype as compared with SCC subtype (P = 0.017). Also, STAT5A and STAT5B immunoexpression was assessed, and the results indicated significantly higher protein levels in NSCLC patients as compared with controls (P = 0.048 and P = 0.034, respectively). High STAT5B immunoexpression was positively correlated with STAT5B gene expression in tumors (rho = 0.755). STAT5B protein level was also significantly higher in T2a+T2b tumors, reflecting high STAT5B gene expression in this group. There was no statistically significant association between mRNA and protein expression levels of the studied genes and patients' characteristics: age, gender, smoking. The obtained results highlight the importance of the genes STAT5B and COX-2 in lung cancer progression.
authors.affiliation: Department of Molecular Bases of Medicine, Medical University of Lodz, Lodz, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Pastuszak-Lewandoska
authors.affiliation: Department of Molecular Bases of Medicine, Medical University of Lodz, Lodz, Poland.
authors.forename: Daria
authors.initials: D
authors.lastname: Domańska
authors.affiliation: Department of Molecular Bases of Medicine, Medical University of Lodz, Lodz, Poland.
authors.forename: Karolina H
authors.initials: KH
authors.lastname: Czarnecka
authors.affiliation: Department of Chest Surgery, General and Oncological Surgery, University Hospital No. 2, Medical University of Lodz, Lodz, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Kordiak
authors.affiliation: Department of Molecular Bases of Medicine, Medical University of Lodz, Lodz, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Migdalska-Sęk
authors.affiliation: Department of Molecular Bases of Medicine, Medical University of Lodz, Lodz, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Nawrot
authors.affiliation: Department of Molecular Bases of Medicine, Medical University of Lodz, Lodz, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Kiszałkiewicz
authors.affiliation: Department of General and Oncological Pulmonology, Medical University of Lodz, Lodz, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Antczak
authors.affiliation: Department of Pneumology and Allergology, Medical University of Lodz, Lodz, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Górski
authors.affiliation: Department of Molecular Bases of Medicine, Medical University of Lodz, Lodz, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Brzeziańska
mesh_terms: D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D051546:Cyclooxygenase 2 / Q000235:genetics* / Q000378:metabolism; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D018832:Molecular Chaperones / Q000235:genetics* / Q000378:metabolism; D009367:Neoplasm Staging; D050828:Protein Inhibitors of Activated STAT / Q000235:genetics* / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012307:Risk Factors; D050799:STAT5 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D012907:Smoking / Q000503:physiopathology
chemical_list: D018832:Molecular Chaperones; C484745:PIAS3 protein, human; D050828:Protein Inhibitors of Activated STAT; D020033:Protein Isoforms; D012333:RNA, Messenger; D050799:STAT5 Transcription Factor; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human