title: Isolation and characterization of plant growth-promoting rhizobacteria and their effects on phytoremediation of petroleum-contaminated saline-alkali soil.
abstract: This study aimed to isolate promising halotolerant and alkalotolerant plant growth-promoting rhizobacteria and to study their effects on the growth of tall fescue and phytodegradation efficiency in a petroleum-contaminated saline-alkaline soil. A total of 115 PGPR strains were isolated from the rhizosphere of tall fescue grown in petroleum-contaminated saline-alkaline soils. Of these, 5 strains indicating 1-aminocyclopropane-l-carboxylic acid deaminase activity>1.0M α-KB mg(-1)h(-1) were selected for further studies. The isolate D5A presented the highest plant-growth-promoting activity and was identified as Klebsiella sp. It grew well on the Luria-Bertani medium containing 9% NaCl and at a pH range of 4-10. A pot experiment was then conducted to study the effect of isolates on phytoremediation. The results showed that inoculation of D5A promoted tall fescue growth and enhanced remediation efficiency in petroleum-contaminated saline-alkaline soil.
authors.affiliation: Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China. Electronic address: liuwuxin@issas.ac.cn.
authors.forename: Wuxing
authors.initials: W
authors.lastname: Liu
authors.affiliation: Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jinyu
authors.initials: J
authors.lastname: Hou
authors.affiliation: Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
authors.forename: Qingling
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Ding
authors.affiliation: Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
authors.forename: Yongming
authors.initials: Y
authors.lastname: Luo
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D019755:Carbon-Carbon Lyases / Q000235:genetics / Q000378:metabolism; D004787:Environmental Pollution / Q000517:prevention & control*; D031751:Festuca / Q000254:growth & development* / Q000378:metabolism / Q000382:microbiology*; D007709:Klebsiella / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D008969:Molecular Sequence Data; D059392:Petroleum Pollution / Q000517:prevention & control*; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D013312:Stress, Physiological
chemical_list: D001426:Bacterial Proteins; D012987:Soil; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases
keywords: Alkalinity; Klebsiella sp.; PGPR; Salinity; Soil contamination