title: Sequential gene silencing using wavelength-selective caged morpholino oligonucleotides.
abstract: Spectrally differentiated caged morpholino oligonucleotides (cMOs) and wavelength-selective illumination have been used to sequentially inactivate organismal gene function. The efficacy of these reverse-genetic chemical probes has been demonstrated in zebrafish embryos, and these reagents have been employed to examine the mechanisms of mesoderm patterning.
authors.affiliation: Departments of Chemical and Systems Biology and Developmental Biology, Stanford University School of Medicine, 269 Campus Drive, CCSR 3155, Stanford, CA 94305 (USA) http://chen.stanford.edu.
authors.forename: Sayumi
authors.initials: S
authors.lastname: Yamazoe
authors.forename: Qingyang
authors.initials: Q
authors.lastname: Liu
authors.forename: Lindsey E
authors.initials: LE
authors.lastname: McQuade
authors.forename: Alexander
authors.initials: A
authors.lastname: Deiters
authors.forename: James K
authors.initials: JK
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D020868:Gene Silencing; D015394:Molecular Structure; D060172:Morpholinos / Q000737:chemistry / Q000235:genetics*; D015027:Zebrafish / Q000196:embryology
chemical_list: D060172:Morpholinos
keywords: antisense agents; cage compounds; developmental biology; gene expression; oligonucleotides