title: Enhanced production of 2,3-butanediol by a genetically engineered Bacillus sp. BRC1 using a hydrolysate of empty palm fruit bunches.
abstract: A Bacillus species that produces 2,3-butanediol (2,3-BD), termed BRC1, was newly isolated, and a 2,3-BD dehydrogenase (Bdh) from this species was identified and characterized at the molecular and biochemical level. Sequence analysis revealed that Bdh is homologous to D-2,3-BD dehydrogenases. An analysis of the enzymatic properties of Bdh overexpressed in Escherichia coli confirmed the molecular results, showing preferred activity toward D-2,3-BD. Optimum pH, temperature, and kinetics determined for reductive and oxidative reactions support the preferential production of 2,3-BD during cell growth. Overexpression of bdh under the control of a xylose-inducible promoter resulted in increased enzyme activity and enhanced 2,3-BD production in Bacillus sp. BRC1. Additionally, a hydrolysate of cellulosic material, (empty palm fruit bunches), was successfully used for the enhanced production of 2,3-BD in the recombinant Bacillus strain.
authors.affiliation: Biorefinery Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk, 580-185, South Korea.
authors.forename: In Yeong
authors.initials: IY
authors.lastname: Kang
authors.forename: Jang Min
authors.initials: JM
authors.lastname: Park
authors.forename: Won-Kyung
authors.initials: WK
authors.lastname: Hong
authors.forename: Yun Seok
authors.initials: YS
authors.lastname: Kim
authors.forename: You Ree
authors.initials: YR
authors.lastname: Jung
authors.forename: Seung-Bum
authors.initials: SB
authors.lastname: Kim
authors.forename: Sun-Yeon
authors.initials: SY
authors.lastname: Heo
authors.forename: Sung-Mok
authors.initials: SM
authors.lastname: Lee
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Kang
authors.forename: Baek-Rock
authors.initials: BR
authors.lastname: Oh
authors.forename: Dae-Hyuk
authors.initials: DH
authors.lastname: Kim
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Seo
authors.forename: Chul Ho
authors.initials: CH
authors.lastname: Kim
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D028023:Arecaceae / Q000382:microbiology*; D001407:Bacillus / Q000145:classification / Q000502:physiology*; D002072:Butylene Glycols / Q000302:isolation & purification* / Q000378:metabolism*; D005638:Fruit / Q000382:microbiology*; D024861:Genetic Enhancement / Q000379:methods; D006868:Hydrolysis; D013045:Species Specificity
chemical_list: D002072:Butylene Glycols; C026978:2,3-butylene glycol; D000429:Alcohol Oxidoreductases; C019801:butanediol dehydrogenase