title: Analysis of the histology of the scar bladder and biochemical parameters of rats with a solitary kidney undergoing immunosuppression with tacrolimus.
abstract: OBJECTIVE
  To evaluate bladder histology in healing and biochemical analysis of rats with single kidney in ischemia/reperfusion, treated with tacrolimus.
  
  
  METHODS
  Fifty rats randomized into five groups. Three rats died in surgery, 47 rats divided in groups: Control (non-operated, n=10), Sham (operated without drugs, n=8), T1 (operated + tacrolimus 1mg/kg, n=10), T2 (operated + tacrolimus 0.1 mg/kg, n=10), T3 (operated + tacrolimus 10mg/kg, n=9). The surgery was: laparotomy, right nephrectomy, left kidney ischemia/reperfusion, cystotomy followed by bladder suture. After that, rats were submited to gavage daily (Control and Sham with saline solution. T1, T2, T3 with tacrolimus in doses already mentioned). On the 14th day, after death induction, cystectomy was performed and bladder was histologicaly analysed. The serum urea, creatinine and tacrolimus were analysed too.
  
  
  RESULTS
  There was difference in serum tacrolimus in T3 compared to the other groups (p<0.05). There was higher doses of creatinine in T3 group and higher urea in groups with tacrolimus. There were significant differences among all histologic variables comparing groups with and without tacrolimus (p<0.05).
  
  
  CONCLUSIONS
  Tacrolimus associated with ischemia/reperfusion is nephrotoxic, suppresses inflammation and seems to delay the healing bladder.
authors.affiliation: School of Medicine, Federal University of Parana, Curitiba, PR, Brazil.
authors.forename: Ricardo Cavalheiro
authors.initials: RC
authors.lastname: Cavalli
authors.affiliation: School of Medicine, UFPR, Curitiba, PR, Brazil.
authors.forename: Renato
authors.initials: R
authors.lastname: Tambara Filho
authors.affiliation: School of Medicine, UFPR, Curitiba, PR, Brazil.
authors.forename: Regina de Paula Xavier
authors.initials: Rde P
authors.lastname: Gomes
authors.affiliation: School of Medicine, UFPR, Curitiba, PR, Brazil.
authors.forename: Djanira Aparecida da Luz
authors.initials: DA
authors.lastname: Veronez
authors.affiliation: School of Medicine, UFPR, Curitiba, PR, Brazil.
authors.forename: Julio
authors.initials: J
authors.lastname: Slongo
authors.affiliation: School of Medicine, UFPR, Curitiba, PR, Brazil.
authors.forename: Rogério de
authors.initials: Rd
authors.lastname: Fraga
mesh_terms: D000818:Animals; D001806:Blood Urea Nitrogen; D002921:Cicatrix / Q000188:drug therapy* / Q000473:pathology; D003404:Creatinine / Q000097:blood; D007166:Immunosuppressive Agents / Q000494:pharmacology / Q000627:therapeutic use*; D007511:Ischemia / Q000150:complications*; D007668:Kidney / Q000098:blood supply*; D008297:Male; D023421:Models, Animal; D009392:Nephrectomy; D011897:Random Allocation; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications; D016559:Tacrolimus / Q000494:pharmacology / Q000627:therapeutic use*; D001743:Urinary Bladder / Q000187:drug effects* / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology
chemical_list: D007166:Immunosuppressive Agents; D003404:Creatinine; D016559:Tacrolimus