title: Facile synthesis of oxidic PEG-modified magnetic polydopamine nanospheres for Candida rugosa lipase immobilization.
abstract: A versatile method for the design of polydopamine-coated magnetic material with a brush-like structure used for Candida Rugosa lipase (CRL) immobilization was reported in this work. First, polydopamine (PDA) was coated on the surface of Fe₃O₄ nanospheres (Fe₃O₄ NPs) with a controllable thickness via dip coating process, and CRL can be immobilized on it directly via covalent bonding. Subsequently, PDA-functionalized Fe₃O₄ NPs were modified with dialdehyde polyethylene glycol (PEG) to obtain the aldehyde groups, and the brush-like structure of the magnetic supports was formed. After being characterized with various methods, it was verified that the prepared magnetic NPs possessed good monodispersity and displayed high saturation magnetization after modification. Meanwhile, the CRL was immobilized on it covalently, and the enzyme activities such as activity, stability, and reusability were investigated. Significantly, the versatility of polydopamine-inspired chemistry combined with the unique biological nature and tunability with dialdehyde PEG could evoke the efficiency of the CRL, making this a promising coating technique for various bio-applications.
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, Lanzhou, 730000, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Hou
authors.forename: Hao
authors.initials: H
authors.lastname: Zhu
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Li
authors.forename: Yijing
authors.initials: Y
authors.lastname: Li
authors.forename: Xinyu
authors.initials: X
authors.lastname: Wang
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhu
authors.forename: Rongde
authors.initials: R
authors.lastname: Zhou
mesh_terms: D002175:Candida / Q000201:enzymology*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005290:Ferric Compounds / Q000737:chemistry; D007211:Indoles / Q000138:chemical synthesis*; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D008280:Magnetics; D054118:Nanospheres / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis*
chemical_list: D004800:Enzymes, Immobilized; D005290:Ferric Compounds; D007211:Indoles; D011108:Polymers; C568283:polydopamine; C000499:ferric oxide; D008049:Lipase