title: [Comparative study of parathormone and vitamin D measurements by three automats: ADVIA Centaur XP® (Siemens), ISYS® (IDS) and Liaison® (Diasorin)].
abstract: Vitamin D (Vit.D) and parathormone (PTH) measurements are usually prescribed for phosphocalcic metabolism assessment and, especially for Vit.D, more and more frequently for other pathologies. In order to step up to automated techniques for these analysis in our laboratory, we tested 3 devices: ADVIA Centaur XP(®) (Siemens), ISYS(®) (IDS) and Liaison(®) (Diasorin), which allow to simultaneously quantify Vit.D and PTH. The aim of this study was to evaluate the fidelity of these methods as well as study the correlation between them and the radioimmunological techniques previously used in our laboratory : « ELSA PTH » (Iba Cisbio International) and « 25-OH D » (IDS). The comparison of PTH analysis was performed on a population of chronic renal failure patients undergoing haemodialysis. According to our study, the 3 devices show acceptable analytical performances; anyway the measurements realized on the ISYS analyzer are the ones showing the best results in terms of fidelity, and the closest results to those obtained with the RIA reference techniques.
authors.affiliation: Département de biochimie et pharmaco-toxicologie, Hôpital de la Cavale Blanche, CHRU, Brest, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Gardien
authors.affiliation: Département de biochimie et pharmaco-toxicologie, Hôpital de la Cavale Blanche, CHRU, Brest, France.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Moineau
authors.affiliation: Département de biochimie et pharmaco-toxicologie, Hôpital de la Cavale Blanche, CHRU, Brest, France, EA 4685, Laboratoire de neurosciences, Faculté de médecine, Brest, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Kerspern
authors.affiliation: EA 4685, Laboratoire de neurosciences, Faculté de médecine, Brest, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Bordron
authors.affiliation: Département de biochimie et pharmaco-toxicologie, Hôpital de la Cavale Blanche, CHRU, Brest, France, EA 4685, Laboratoire de neurosciences, Faculté de médecine, Brest, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Carre
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D004867:Equipment Design; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010281:Parathyroid Hormone / Q000097:blood*; D015203:Reproducibility of Results; D014807:Vitamin D / Q000097:blood*
chemical_list: D010281:Parathyroid Hormone; D014807:Vitamin D
keywords: immunoassay; parathormone; vitamin D