title: A cost-effective Z-folding controlled liquid handling microfluidic paper analysis device for pathogen detection via ATP quantification.
abstract: A cost-effective microfluidic paper analysis device (μPAD) was developed with a special Z-folding design for controlling the fluidic flowing and substrate transportation. This presented μPAD can be easily fabricated through wax printing by using a solid ink printer which deposits wax onto the surface of a chromatographic paper, and then baked on a hotplate by penetrating the molten wax into the paper to create a hydrophobic barrier. After μPAD fabrication, liquid control and substrate transportation can be easily carried out by twice folding the μPAD following Z shape. The Z folding made two separated reagent holding zone connected while the detection reaction occurred with the connection. In this paper, a pathogens detection indicated by ATP quantification was took as a proof-in-principle application of using this presented μPAD, the limit of detection (LOD) was 1 μM for ATP detection and 2.6×10(7) CFU/mL for Salmonella live cell detection, which showed a great potential for Point-of-Care Testing (POCT) applications.
authors.affiliation: Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Sheng-Quan
authors.initials: SQ
authors.lastname: Jin
authors.affiliation: Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Su-Miao
authors.initials: SM
authors.lastname: Guo
authors.affiliation: Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. Electronic address: pzuo@ecust.edu.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Zuo
authors.affiliation: Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. Electronic address: bcye@ecust.edu.cn.
authors.forename: Bang-Ce
authors.initials: BC
authors.lastname: Ye
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000295:instrumentation*; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques; D044085:Microfluidics / Q000295:instrumentation*; D011327:Printing; D012475:Salmonella / Q000302:isolation & purification* / Q000472:pathogenicity
chemical_list: D000255:Adenosine Triphosphate
keywords: ATP detection; Liquid control.; Microfluidic paper analysis device; Pathogen detection