title: Evolutionary history and population genetic structure of the endemic tree frog Hyla tsinlingensis (Amphibia: Anura: Hylidae) inferred from mitochondrial gene analysis.
abstract: The influence of topography and Pleistocenic climatic fluctuations on the population genetic structure of amphibians in the Tsinling-Dabieshan Mountains of China is poorly investigated. Hyla tsinlingensis is a tree frog endemic to the Tsinling-Dabieshan Mountains, with a restricted and patchy distribution that is currently shrinking. We speculated on the evolutionary history of amphibians in this region by studying the population genetic structure of H. tsinlingensis. Using a total of 212 samples, 32 haplotypes and four haplogroups were found in the present study. Population genetic structure showed significant differentiation (F(ST)) between most populations of H. tsinlingensis in the Tsinling-Dabieshan Mountains. An analysis of molecular variance (AMOVA) suggested that most of the observed genetic variation occurs between the two regions (the Tsinling and Dabieshan Mountains). Mantel tests indicated that the genetic divergence was induced through isolation by distance. Using Monmonier's maximum difference algorithm to predict the genetic barrier, two putative barriers in gene flow that separate lineages of H. tsinlingensis were identified. Mismatch distribution and neutrality tests found a sudden population expansion in all haplogroups except the Tsinling population and total population. This population expansion was identified between 0.5 Myr to 0.1 Myr (Quaternary) by Bayesian skyline plot (BSP). Divergence dating indicated the divergence time between the Tsinling population and Dabieshan population to be 3.26 MYA (Pliocene). In conclusion, the topography of the Tsinling and Dabieshan Mountains exerts a significant impact on the population genetic structure of H. tsinlingensis, and climatic oscillations during glacial periods in the Quaternary affected the distribution of H. tsinlingensis.
authors.affiliation: a College of Life Sciences, Shaanxi Normal University , Xi'an , P.R. China and.
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Zhang
authors.affiliation: a College of Life Sciences, Shaanxi Normal University , Xi'an , P.R. China and.|b Department of cell biology and genetics , Zunyi Medical University , Zunyi , P.R. China.
authors.forename: Yan-Yu
authors.initials: YY
authors.lastname: Zhao
authors.affiliation: b Department of cell biology and genetics , Zunyi Medical University , Zunyi , P.R. China.
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Li
authors.affiliation: a College of Life Sciences, Shaanxi Normal University , Xi'an , P.R. China and.
authors.forename: Xiao-Chen
authors.initials: XC
authors.lastname: Li
mesh_terms: D000818:Animals; D001001:Anura / Q000145:classification / Q000235:genetics*; D019143:Evolution, Molecular; D050259:Genes, Mitochondrial; D049810:Genetic Speciation; D010802:Phylogeny; D058974:Phylogeography
keywords: Climatic oscillation; Hyla tsinlingensis; evolutionary history; population genetic structure; topography