title: Role of peripheral purinoceptors in the development of bee venom-induced nociception: a behavioural and electrophysiological study in rats.
abstract: Colocalization of purinergic P2X and P2Y receptors in dorsal root ganglion sensory neurons implies that these receptors play an integrative role in the nociceptive transmission process under inflammatory conditions. In the present study, behavioural and in vivo electrophysiological methods were used to examine the peripheral role of P2 receptors in the persistent nociceptive responses induced by subcutaneous bee venom injection (2 mg/mL) in. Sprague-Dawley rats Local pretreatment with the wide-spectrum P2 receptor antagonist pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS; 1 mmol/L, 50 μL) 10 min prior to s.c. bee venom injection significantly suppressed the duration of spontaneous nociceptive lifting/licking behaviour, inhibited mechanical hyperalgesia and decreased the firing of spinal dorsal horn wide dynamic range neurons in response to bee venom, without affecting primary thermal and mirror-image hyperalgesia. The localized antinociceptive action of PPADS was not due to a systemic effect, because application of the same dose of PPADS to the contralateral side was not effective. The results suggest that activation of peripheral P2 receptors is involved in the induction of nociceptive responses, mechanical hyperalgesia and the excitation of sensory spinal neurons.
authors.affiliation: Department of Physiology and Pathophysiology, School of Medicine, Xi'an Jiaotong University Health Science Center, Xi'an, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Rong
authors.initials: R
authors.lastname: Luo
authors.forename: Pei
authors.initials: P
authors.lastname: Fan
authors.forename: Yuan
authors.initials: Y
authors.lastname: Guo
authors.forename: Hui-Sheng
authors.initials: HS
authors.lastname: Wang
authors.forename: Shao-Jie
authors.initials: SJ
authors.lastname: Ma
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001514:Bee Venoms / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects* / Q000502:physiology; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism; D008297:Male; D059226:Nociceptive Pain / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology*; D010147:Pain Measurement; D017288:Pain Threshold / Q000187:drug effects; D058920:Purinergic P2X Receptor Antagonists / Q000494:pharmacology; D058921:Purinergic P2Y Receptor Antagonists / Q000494:pharmacology; D017207:Rats, Sprague-Dawley; D058469:Receptors, Purinergic P2X / Q000378:metabolism / Q000502:physiology*; D058470:Receptors, Purinergic P2Y / Q000378:metabolism / Q000502:physiology*; D011984:Sensory Receptor Cells / Q000187:drug effects* / Q000378:metabolism
chemical_list: D001514:Bee Venoms; D058920:Purinergic P2X Receptor Antagonists; D058921:Purinergic P2Y Receptor Antagonists; D058469:Receptors, Purinergic P2X; D058470:Receptors, Purinergic P2Y
keywords: ATP; P2 receptors; Sprague-Dawley rats; dorsal horn neurons; hyperalgesia