title: Identification of newly isolated Talaromyces pinophilus and statistical optimization of β-glucosidase production under solid-state fermentation.
abstract: Fungi able to degrade agriculture wastes were isolated from different soil samples, rice straw, and compost; these isolates were screened for their ability to produce β-glucosidase. The most active fungal isolate was identified as Talaromyces pinophilus strain EMOO 13-3. The Plackett-Burman design is used for identifying the significant variables that influence β-glucosidase production under solid-state fermentation. Fifteen variables were examined for their significances on the production of β-glucosidase in 20 experimental runs. Among the variables screened, moisture content, Tween 80, and (NH4)2SO4 had significant effects on β-glucosidase production with confidence levels above 90% (p < 0.1). The optimal levels of these variables were further optimized using Box-Behnken statical design. As a result, the maximal β-glucosidase activity is 3648.519 U g(-1), which is achieved at the following fermentation conditions: substrate amount 0.5 (g/250 mL flask), NaNO3 0.5 (%), KH2PO4 0.3 (%), KCl 0.02 (%), MgSO4 · 7H2O 0.01 (%), CaCl2 0.01 (%), yeast extract 0.07 (%), FeSO4 · 7H2O 0.0002 (%), Tween 80 0.02 (%), (NH4)2SO4 0.3 (%), pH 6.5, temperature 25°C, moisture content 1 (mL/g dry substrate), inoculum size 0.5 (mL/g dry substrate), and incubation period 5 days.
authors.affiliation: a Department of Bioprocess Development , Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications , Alexandria , Egypt.
authors.forename: Noura El-Ahmady
authors.initials: Nel-A
authors.lastname: El-Naggar
authors.forename: S A
authors.initials: SA
authors.lastname: Haroun
authors.forename: Eman A
authors.initials: EA
authors.lastname: Oweis
authors.forename: A A
authors.initials: AA
authors.lastname: Sherief
mesh_terms: D002244:Carbon / Q000737:chemistry; D003470:Culture Media; D005285:Fermentation; D009584:Nitrogen / Q000737:chemistry; D032901:Talaromyces / Q000737:chemistry / Q000201:enzymology*; D013696:Temperature; D001617:beta-Glucosidase / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D002244:Carbon; D001617:beta-Glucosidase; D009584:Nitrogen
keywords: Plackett–Burman and Box–Behnken design; Talaromyces pinophilus; identification; optimization; solid–state fermentation; β-glucosidase production