title: Associations of single nucleotide polymorphisms related to insulin resistance with polycystic ovary syndrome.
abstract: BACKGROUND
  We investigated and validated most of the hitherto-suggested polymorphisms for PCOS.
  
  
  METHODS
  DNA samples from 359 pre-menopausal Korean women with PCOS (n=196) and without (n=166) were analyzed by primer extension and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry using iPLEX Gold technology from Sequenom (Sequenom Inc, San Diego, CA, USA) to determine the genotypic frequency of 80 single nucleotide polymorphisms (SNPs) from the 49 genes.
  
  
  RESULTS
  Four SNPs (rs10060745, rs1719889, rs680, rs705379) showed moderate association with a risk of PCOS. Three (rs1719889, rs680, and rs705379) out of four SNPs were related to insulin resistance. The rs705379 showed significant association with increased fasting glucose (p=0.045) and serum 2-h C-peptide level (p=0.026). The rs1719889 showed significant association with fasting glucose level (p=0.03).
  
  
  CONCLUSIONS
  Our results provide evidence for the relationship between high prevalence of insulin resistance and PCOS.
authors.affiliation: Department of Laboratory Medicine.
authors.forename: Hee-Yeon
authors.initials: HY
authors.lastname: Woo
authors.affiliation: Department of Obstetrics and Gynecology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea khmd.kim@samsung.com.
authors.forename: Kye-Hyun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Seung-Tae
authors.initials: ST
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Kwon
authors.affiliation: Department of Laboratory Medicine.
authors.forename: Hyosoon
authors.initials: H
authors.lastname: Park
mesh_terms: D000328:Adult; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics*; D011085:Polycystic Ovary Syndrome / Q000235:genetics* / Q000473:pathology; D020641:Polymorphism, Single Nucleotide; D056910:Republic of Korea; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
keywords: Insulin resistance; Polycystic ovarian syndrome; Single nucleotide polymorphism