title: [Sequence analysis of a novel allele HLA-C*01:78].
abstract: OBJECTIVE
  To investigate the genetic basis for a novel allele HLA-C*01:78.
  
  
  METHODS
  Genomic DNA was extracted from peripheral blood using a QIAGEN quick DNA extraction kit. The regions encompassing HLA-C from exon 1 to intron 3 and intron 3 to 3'UTR were amplified and cloned using a cloning sequencing kit in order to split the two alleles apart. Selected clones were sequenced to include exons 2 to 4.
  
  
  RESULTS
  Sequencing results have indicated the HLA-C alleles of the proband to be a novel C*03:04 allele. The sequence has been submitted to GenBank (KF049216). BLAST analysis has confirmed the novel allele to have one nucleotide difference as C*01:03 at genomic nt316C>A (codon 82CGC>AGC) in exon 2, which has resulted in replacement of one amino acid (82R>S).
  
  
  CONCLUSIONS
  The novel allele has been officially named as C*01:78 by the WHO Nomenclature Committee. The HLA allele type of the proband was therefore A*02:07, 24:02; B*40:01, 46:01; C*01:78, 03:04; DQB1*05:02, 05:02; DRB1*16:02, 16:02.
authors.affiliation: Immunogenetics Laboratory, Shenzhen Blood Center, Shenzhen, Guangdong 518035, P.R. China. shallylizhen@aliyun.com.
authors.forename: Daming
authors.initials: D
authors.lastname: Wang
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zou
authors.forename: Yunping
authors.initials: Y
authors.lastname: Xu
authors.forename: Jianxin
authors.initials: J
authors.lastname: Zhen
authors.forename: Liumei
authors.initials: L
authors.lastname: He
authors.forename: Suqing
authors.initials: S
authors.lastname: Gao
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D005091:Exons; D005260:Female; D015236:HLA-C Antigens / Q000235:genetics*; D006801:Humans; D007438:Introns; D007938:Leukemia / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D015236:HLA-C Antigens