title: Expression of placenta growth factor, soluble fms-like tyrosine kinase-1, metal-responsive transcription factor-1, heme oxygenase 1 and hypoxia inducible factor-1α mRNAs in pre-eclampsia placenta and the effect of pre-eclampsia sera on their expression of choriocarcinoma cells.
abstract: OBJECTIVE
  We studied the effect of pre-eclampsia sera on the expression of placenta growth factor (PlGF), soluble fms-like tyrosine kinase-1 (sFlt-1), metal-responsive transcription factor-1 (MTF-1), heme oxygenase 1 (HO-1) and hypoxia inducible factor-1α (HIF-1α) mRNAs in JEG-3 cells (trophoblast-derived cells) and placenta from pre-eclampsia patients to investigate pre-eclampsia pathophysiology.
  
  
  METHODS
  Placenta and serum samples were taken from pre-eclampsia and normal pregnancy patients. JEG-3 cells were cultured with pre-eclampsia and normal pregnant sera in 24-well tissue culture plates. RNA was purified from placental trophoblast cells and JEG-3 cells 24 h after incubation. The expression of mRNA was measured using real-time polymerase chain reaction.
  
  
  RESULTS
  The expression of sFlt-1 mRNA increased, and that of PlGF and HO-1 mRNA decreased in JEG-3 cells after incubation with pre-eclampsia sera. The expression of PlGF mRNA decreased, and that of sFlt-1mRNA increased in pre-eclampsia placenta. The expression of MTF-1 and HO-1 mRNA decreased. A correlation was found between PlGF mRNA expression and the expression of MTF-1 and HIF-1α mRNA. A correlation between sFlt-1 and HIF-1α mRNA expression was also found.
  
  
  CONCLUSIONS
  Changes in PlGF mRNA expression in pre-eclampsia placenta may relate to serum factors and the expression of MTF-1 and HIF-α mRNA. Changes in sFlt-1mRNA expression may relate to serum factors and the expression of HIF-α mRNA. We suggest that serum factors play a role in PlGF and sFlt-1 expression in pre-eclampsia placenta.
authors.affiliation: Department of Obstetrics and Gynecology, Nihon University School of Medicine, Tokyo, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Maebayashi Asanuma
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Hiromitu
authors.initials: H
authors.lastname: Azuma
authors.forename: Erina
authors.initials: E
authors.lastname: Kato
authors.forename: Noriko
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Takayuki
authors.initials: T
authors.lastname: Murase
authors.forename: Fumihisa
authors.initials: F
authors.lastname: Chishima
authors.forename: Manami
authors.initials: M
authors.lastname: Suzuki
mesh_terms: D000328:Adult; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism; D010920:Placenta / Q000201:enzymology / Q000378:metabolism*; D000072483:Placenta Growth Factor; D011225:Pre-Eclampsia / Q000097:blood / Q000201:enzymology / Q000378:metabolism*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012995:Solubility; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000097809:Transcription Factor MTF-1
chemical_list: D004268:DNA-Binding Proteins; D051547:Heme Oxygenase-1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D000072483:Placenta Growth Factor; D011257:Pregnancy Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014157:Transcription Factors; D040281:Vascular Endothelial Growth Factor Receptor-1; D000097809:Transcription Factor MTF-1; C497442:HIF1A protein, human; C000608036:PGF protein, human; C496541:HMOX1 protein, human
keywords: heme oxygenase 1; metal-responsive transcription factor-1; placenta growth factor; pre-eclampsia; soluble fms-like tyrosine kinase-1