title: Multiplex single particle analysis in microfluidics.
abstract: A straightforward way to measure separated micrometric sized particles in microfluidic flow is reported. The light scattering profile (LSP) of each single particle is fully characterized by using a CMOS-camera based small angle light scattering (SALS) apparatus, ranging from 2° up to 30°. To ensure controlled particle passage through the incident laser, a viscoelastic 3D alignment effect by viscoelastic induced particle migration has been implemented in a simple and cost-effective microfluidic device. Different polystyrene particle sizes are measured in microfluidic flows and the obtained scattering signatures are matched with the Lorenz-Mie based scattering theory. The results confirm the possibility of using this apparatus for real multiplex particle analyses in microfluidic particle flows.
authors.affiliation: Center for Advanced Biomaterials for Healthcare@CRIB, Istituto Italiano di Tecnologia (IIT), Largo Barsanti e Matteucci 53, 80125 Naples, Italy. david.dannhauser@iit.it.
authors.forename: D
authors.initials: D
authors.lastname: Dannhauser
authors.forename: G
authors.initials: G
authors.lastname: Romeo
authors.forename: F
authors.initials: F
authors.lastname: Causa
authors.forename: I
authors.initials: I
authors.lastname: De Santo
authors.forename: P A
authors.initials: PA
authors.lastname: Netti
mesh_terms: D004548:Elasticity; D008027:Light; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry*; D012031:Refractometry; D012542:Scattering, Radiation; D014783:Viscosity
chemical_list: D011137:Polystyrenes