title: Identification, immunolocalization, and characterization analyses of an exopeptidase of papain superfamily, (cathepsin C) from Clonorchis sinensis.
abstract: Cathepsin C is an important exopeptidase of papain superfamily and plays a number of great important roles during the parasitic life cycle. The amino acid sequence of cathepsin C from Clonorchis sinensis (C. sinensis) showed 54, 53, and 49% identities to that of Schistosoma japonicum, Schistosoma mansoni, and Homo sapiens, respectively. Phylogenetic analysis utilizing the sequences of papain superfamily of C. sinensis demonstrated that cathepsin C and cathepsin Bs came from a common ancestry. Cathepsin C of C. sinensis (Cscathepsin C) was identified as an excretory/secretory product by Western blot analysis. The results of transcriptional level and translational level of Cscathepsin C at metacercaria stage were higher than that at adult worms. Immunolocalization analysis indicated that Cscathepsin C was specifically distributed in the suckers (oral sucker and ventral sucker), eggs, vitellarium, intestines, and testis of adult worms. In the metacercaria, it was mainly detected on the cyst wall and excretory bladder. Combining with the results mentioned above, it implies that Cscathepsin C may be an essential proteolytic enzyme for proteins digestion of hosts, nutrition assimilation, and immune invasion of C. sinensis. Furthermore, it may be a potential diagnostic antigen and drug target against C. sinensis infection.
authors.affiliation: Department of Pathogen Biology, Hainan Medical College, Haikou, Hainan, 571199, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Liang
authors.forename: Lei
authors.initials: L
authors.lastname: He
authors.forename: Yanquan
authors.initials: Y
authors.lastname: Xu
authors.forename: Xueqing
authors.initials: X
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Mengyu
authors.initials: M
authors.lastname: Ren
authors.forename: Chi
authors.initials: C
authors.lastname: Liang
authors.forename: Xuerong
authors.initials: X
authors.lastname: Li
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.forename: Gang
authors.initials: G
authors.lastname: Lu
authors.forename: Xinbing
authors.initials: X
authors.lastname: Yu
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D020789:Cathepsin C / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002415:Cats; D003001:Cloning, Molecular; D003003:Clonorchiasis / Q000469:parasitology*; D003004:Clonorchis sinensis / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D019295:Computational Biology; D003530:Cyprinidae / Q000469:parasitology; D020689:Exopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D058488:Metacercariae; D008961:Models, Structural; D008969:Molecular Sequence Data; D010206:Papain / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D051381:Rats; D016415:Sequence Alignment
chemical_list: D000909:Antibodies, Helminth; D020689:Exopeptidases; D020789:Cathepsin C; D010206:Papain