title: Characterisation of tissue transglutaminase-reactive T cells from patients with coeliac disease and healthy controls.
abstract: Previous studies have shown evidence for T lymphocytes specific for tissue transglutaminase (tTG) in the periphery of coeliac disease (CD) patients. These cells could play a role in disease pathogenesis and may be involved in providing help for the production of anti-tTG autoantibodies. The objective of this study was to further investigate the presence of tTG-specific T cells in patients with treated and untreated CD, and normal controls. Positive proliferative responses to three different commercial tTG antigens were detected in all groups tested, occurring more frequently and at higher levels in untreated CD patients. The addition of antibodies to HLA-DQ and HLA-DR caused a significant reduction in the proliferative response to tTG. T cell lines specific for tTG and composed predominantly of CD4-positive T cells were generated from responsive CD and control individuals, and were found to produce large amounts of interferon-γ, as well as interleukins 10, 17A, and 21.
authors.affiliation: National Children's Research Centre, Our Lady's Hospital For Sick Children, Crumlin Dublin 12, Ireland; Institute of Molecular Medicine, Trinity College Dublin Dublin 8, Ireland; Department of Immunology, St James's Hospital, Dublin 8, Ireland. Electronic address: comerfor@tcd.ie.
authors.forename: Ross
authors.initials: R
authors.lastname: Comerford
authors.affiliation: Institute of Molecular Medicine, Trinity College Dublin Dublin 8, Ireland; Department of Immunology, St James's Hospital, Dublin 8, Ireland.
authors.forename: Christian
authors.initials: C
authors.lastname: Coates
authors.affiliation: Dublin Institute of Technology, Kevin Street, Dublin 8, Ireland.
authors.forename: Greg
authors.initials: G
authors.lastname: Byrne
authors.affiliation: Dublin Institute of Technology, Kevin Street, Dublin 8, Ireland.
authors.forename: Sara
authors.initials: S
authors.lastname: Lynch
authors.affiliation: Institute of Molecular Medicine, Trinity College Dublin Dublin 8, Ireland.
authors.forename: Padraic
authors.initials: P
authors.lastname: Dunne
authors.affiliation: Institute of Molecular Medicine, Trinity College Dublin Dublin 8, Ireland.
authors.forename: Margaret
authors.initials: M
authors.lastname: Dunne
authors.affiliation: National Children's Research Centre, Our Lady's Hospital For Sick Children, Crumlin Dublin 12, Ireland.
authors.forename: Jacinta
authors.initials: J
authors.lastname: Kelly
authors.affiliation: Institute of Molecular Medicine, Trinity College Dublin Dublin 8, Ireland; Department of Immunology, St James's Hospital, Dublin 8, Ireland.
authors.forename: Conleth
authors.initials: C
authors.lastname: Feighery
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002446:Celiac Disease / Q000209:etiology / Q000276:immunology / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D019204:GTP-Binding Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007378:Interleukins / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000201:enzymology* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D055815:Young Adult
chemical_list: D007378:Interleukins; D007371:Interferon-gamma; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins
keywords: Autoimmunity; Coeliac disease; Interferon-γ; Interleukin-17A; T cells; Tissue transglutaminase