title: Infiltration of alternatively activated macrophages in cancer tissue is associated with MDSC and Th2 polarization in patients with esophageal cancer.
abstract: Myeloid derived suppressor cells (MDSCs) expand in cancer bearing hosts and contribute to tumor immune evasion. M2 macrophages constitute a major cellular component of cancer-related inflammation. However, the correlation between circulating MDSCs and infiltrating M2 macrophages in tumor tissues from patients with esophageal cancer (ECA), and its potential relationship with the polarization of Th2 cells remain unclear. In the present study, we showed the level of MDSCs in PBMC and Arg1 in plasma were significantly elevated in ECA patients, and the increased ratio of MDSC in PBMC was closely related to the expression of CD163 in cancer tissues. In addition, the ECA patients exhibited remarkable increases in the mRNA levels of IL-4 and GATA3, as well as the protein levels of IL-13 and IL-6, but IFN-γ and IL-12 in peripheral blood were decreased. Our data indicate that the increased Th2 cytokines are associated with MDSCs and M2 macrophages polarization, and foster the infiltration of CD163+M2 macrophages in cancer tissues, which promote the formation of immunosuppressive microenvironment in ECA patients.
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, P. R. China; Department of Laboratory Medicine, Suzhou Hospital Affiliated to Nanjing Medical University, Suzhou, P. R. China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, P. R. China.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, P. R. China.
authors.forename: Zhaoliang
authors.initials: Z
authors.lastname: Su
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, P. R. China.
authors.forename: Prince
authors.initials: P
authors.lastname: Amoah Barnie
authors.affiliation: Department of Laboratory Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang, P. R. China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Jiao
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, P. R. China.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Bie
authors.affiliation: Department of Pathology and Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, P. R. China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Lu
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, P. R. China.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, P. R. China.
authors.forename: Huaxi
authors.initials: H
authors.lastname: Xu
mesh_terms: D016764:Cell Polarity / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D004938:Esophageal Neoplasms / Q000276:immunology* / Q000378:metabolism*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D018793:Interleukin-13 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D022423:Myeloid Cells / Q000166:cytology* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D018418:Th2 Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D018793:Interleukin-13; D015850:Interleukin-6; D018664:Interleukin-12; D007371:Interferon-gamma