title: Postoperative changes in amniotic membrane as a carrier for allogeneic cultured limbal epithelial transplantation.
abstract: OBJECTIVE
  To investigate the morphologic changes and outcomes of the amniotic membrane as a carrier for allogeneic cultivated limbal epithelial transplantation.
  
  
  METHODS
  Prospective, noncomparative, interventional study.
  
  
  METHODS
  A total of 16 eyes receiving allogeneic cultivated limbal epithelial transplantation with amniotic membrane as a carrier were enrolled. Morphologic changes in the amniotic membrane were observed by confocal microscopy and RTVue optical coherence tomography. The paired t test was employed to compare the mean best corrected visual acuity (BCVA) and corneal stromal thickness.
  
  
  RESULTS
  Of the 16 eyes, 12 had stable ocular surfaces (group A), while the other 4 eyes had failed surgeries due to immune rejection (group B). Confocal microscopy showed residual amniotic membrane tissues in 8 eyes in group A at 1 year. However, the amniotic membrane was not detected in group B at 8-10 months. RTVue optical coherence tomography showed discontinuous amniotic membrane tissues in all eyes in group A at 1 year, while highly reflective opacity was seen in the corneal stroma in group B. There were no statistically significant differences in mean BCVA and corneal stromal thickness in group A at 1 month and 1 year after transplantation (P > 0.05), but the mean BCVA showed a statistically significant difference at 1 month and after the disappearance of the amniotic membrane in group B (P < 0.05).
  
  
  CONCLUSIONS
  For eyes with stable ocular surfaces after cultivated limbal epithelial transplantation, the amniotic membrane can be present in the cornea for at least 1 year, with no impact on visual acuity or corneal stromal thickness. Chronic inflammation and neovascularization on the ocular surface may accelerate the disappearance of the amniotic membrane.
authors.affiliation: Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Qi
authors.affiliation: Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China.
authors.forename: Junyi
authors.initials: J
authors.lastname: Wang
authors.affiliation: Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Sun
authors.affiliation: Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China.
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China. Electronic address: lixin_xie@hotmail.com.
authors.forename: Lixin
authors.initials: L
authors.lastname: Xie
mesh_terms: D000293:Adolescent; D000328:Adult; D000650:Amnion / Q000473:pathology*; D002478:Cells, Cultured; D003316:Corneal Diseases / Q000473:pathology / Q000601:surgery*; D004847:Epithelial Cells / Q000637:transplantation*; D005260:Female; D006801:Humans; D016850:Limbus Corneae / Q000166:cytology*; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged; D011184:Postoperative Period; D011446:Prospective Studies; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D054457:Tissue Scaffolds; D041623:Tomography, Optical Coherence; D014184:Transplantation, Homologous; D055815:Young Adult