title: Effect of simvastatin on expression of IL17, HMGB1 and TLR4 in LN kidney tissues of rats.
abstract: OBJECTIVE
  To observe the intervention influence and effect of simvastatin on the expression of interleukin 17 (LI17), high mobility group protein 1 (HMGB1) and TLR4 path in Lupus nephritis (LN) rats.
  
  
  METHODS
  A total of 28 BSXSB male mice with LN (16 weeks) were randomly divided into observation group and the comparison group, observation group was given 6 mg•kg(-1)•d(-1) simvastatin in 0.1% PBS lavage for 4 weeks, the comparison group was not given any treatment. Blood urea nitrogen (BUN) level and urine trace albumin (Scr) level of two groups were determined. The expression of IL17, HMGB1 and TLR4 protein was detected using immune histochemical method, and the kidney histological damage was observed.
  
  
  RESULTS
  BNU, LI17, HMGB1, TLR4 protein and HMGB1 mRNA in observation group was significantly lower than that in control group (P<0.05); There was no statistical difference of Scr level between two groups (P>0.05). Histological observation showed glomerular lesions integral of observation group was obviously lower than that of control group.
  
  
  CONCLUSIONS
  Simvastatin can reduce the expression of IL17, HMGB1 and TLR4 protein in LN mice, thereby can inhibit the autoimmune response as a potential treatment function of LN.
authors.affiliation: Urology Department, Shanghai Pudong Hospital of traditional Chinese medicine, Shanghai 201299, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Urology Department, Shuguang Hospital affiliated to Shanghai Traditional Chinese Medicine University, Shanghai 201203, China.
authors.forename: Ya-Ting
authors.initials: YT
authors.lastname: Sun
authors.affiliation: Urology Department, Shanghai Pudong Hospital of traditional Chinese medicine, Shanghai 201299, China.
authors.forename: Lin-Xia
authors.initials: LX
authors.lastname: Xia
authors.affiliation: Urology Department, Shuguang Hospital affiliated to Shanghai Traditional Chinese Medicine University, Shanghai 201203, China.
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Zhang
authors.affiliation: Urology Department, Shuguang Hospital affiliated to Shanghai Traditional Chinese Medicine University, Shanghai 201203, China. Electronic address: qinytg1973@163.com.
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Yang
keywords: Expression; HMGB1; IL17; Lupus nephritis; Simvastatin; TLR4