title: Binding of a monoclonal antibody to the phospholamban cytoplasmic domain interferes with the channel activity of phospholamban reconstituted in a tethered bilayer lipid membrane.
abstract: Phospholamban (PLN), a membrane protein present in the sarcoplasmic reticulum of cardiac myocytes, is a crucial regulator of cardiac function. It is known that PLN appears as a monomer and as a pentamer. However, the role of the PLN pentamer and its ability to generate an ion channel are a matter of debate. To address this issue we employed an experimental approach that combines electrochemical impedance spectroscopy and surface plasmon resonance measurements. In particular, we investigated the channel activity of wild-type PLN reconstituted in a tethered bilayer lipid membrane (tBLM) on a gold surface. Our results indicate that reconstituted PLN can generate ion-conducting channels in a tBLM. Experiments with a PLN monoclonal antibody support an oriented incorporation of PLN in the tBLM. We show that the binding of the antibody to the PLN cytoplasmic domain interferes with PLN channel activity.
authors.affiliation: Department of Chemistry "Ugo Schiff", University of Florence , 50019 Sesto Fiorentino, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Smeazzetto
authors.forename: Alessio
authors.initials: A
authors.lastname: Sacconi
authors.forename: Adrian L
authors.initials: AL
authors.lastname: Schwan
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Margheri
authors.forename: Francesco
authors.initials: F
authors.lastname: Tadini-Buoninsegni
mesh_terms: D000911:Antibodies, Monoclonal / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D008051:Lipid Bilayers; D020349:Surface Plasmon Resonance; D000099211:Phospholamban
chemical_list: D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins; D008051:Lipid Bilayers; D000099211:Phospholamban