title: HPLC-pDA simultaneous determination and protective effect of Anemarrhena asphodeloides against acute renal failure.
abstract: We investigated the protective effects against acute renal failure (ARF) of Anemarrhena asphodeloides (AA) and performed simultaneous analysis of three compounds, neomangiferin (1), mangiferin (2), and isomangiferin (3) in AA using a high-performance liquid chromatography-photodiode array. To measure the protective effect of ARF, the levels of reactive oxygen species (ROS) and glutathione depletion were determined using a kit. HPLC analysis was performed using a Gemini Cia column at 40 degrees C. The mobile phase used gradient elution with 1.0% (v/v) aqueous acetic acid (A) and 1.0% (v/v) acetic acid in acetonitrile (B). The flow rate was 1.0 mL/min. In our assay, AA extract inhibits cisplatin-induced production of intracellular ROS. Pre-incubation of AA extracts (10-200 microg/mL) markedly maintained cell viability compared with controls in the noncisplatin-treated cells. Calibration curves of all compounds showed good linearity (r2 > or = 0.9992). Recoveries of the three compounds were 98.9-103.4%. The relative standard deviations of intra- and inter-day precision were 0.07-1.73% and 0.12-1.49%, respectively. The amounts of the three components were 1.22-20.63 mg/g. The AA extract has potential as a therapeutic agent for treatment of ARF. In addition, the established method will help to improve quality control of AA.
authors.forename: Chang-Seob
authors.initials: CS
authors.lastname: Seo
authors.forename: Hyekyung
authors.initials: H
authors.lastname: Ha
authors.forename: Young-Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Jungb
mesh_terms: D031403:Anemarrhena / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002945:Cisplatin / Q000633:toxicity; D005978:Glutathione / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal; D017382:Reactive Oxygen Species / Q000378:metabolism; D051437:Renal Insufficiency / Q000517:prevention & control*
chemical_list: D010936:Plant Extracts; D017382:Reactive Oxygen Species; D005978:Glutathione; D002945:Cisplatin