title: Infarct volume prediction using apparent diffusion coefficient maps during middle cerebral artery occlusion and soon after reperfusion in the rat.
abstract: Middle cerebral artery occlusion (MCAO) in rodents causes brain infarctions of variable sizes that depend on multiple factors, particularly in models of ischemia/reperfusion. This is a major problem for infarct volume comparisons between different experimental groups since unavoidable variability can induce biases in the results and imposes the use of large number of subjects. MRI can help to minimize these difficulties by ensuring that the severity of ischemia is comparable between groups. Furthermore, several studies showed that infarct volumes can be predicted with MRI data obtained soon after ischemia onset. However, such predictive studies require multiparametric MRI acquisitions that cannot be routinely performed, and data processing using complex algorithms that are often not available. The aim here was to provide a simplified method for infarct volume prediction using apparent diffusion coefficient (ADC) data in a model of transient MCAO in rats. ADC images were obtained before, during MCAO and after 60 min of reperfusion. Probability histograms were generated using ADC data obtained either during MCAO, after reperfusion, or both combined. The results were compared to real infarct volumes, i.e.T2 maps obtained at day 7. Assessment of the performance of the estimations showed better results combining ADC data obtained during occlusion and at reperfusion. Therefore, ADC data alone can provide sufficient information for a reasonable prediction of infarct volume if the MRI information is obtained both during the occlusion and soon after reperfusion. This approach can be used to check whether drug administration after MRI acquisition can change infarct volume prediction.
authors.affiliation: CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain. Electronic address: raul.tudela@ub.edu.
authors.forename: Raúl
authors.initials: R
authors.lastname: Tudela
authors.affiliation: Experimental MRI 7T Unit, IDIBAPS, Barcelona, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Soria
authors.affiliation: Department of Brain Ischemia and Neurodegeneration, Institut d'Investigacions Biomèdiques de Barcelona (IIBB)-Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain; IDIBAPS, Barcelona, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Pérez-De-Puig
authors.affiliation: CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain; Biophysics and Bioengineering Laboratory, University of Barcelona, Barcelona, Spain.
authors.forename: Domènec
authors.initials: D
authors.lastname: Ros
authors.affiliation: CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain; Nuclear Medicine Department, Hospital Clínic, Barcelona, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Pavía
authors.affiliation: Department of Brain Ischemia and Neurodegeneration, Institut d'Investigacions Biomèdiques de Barcelona (IIBB)-Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain; IDIBAPS, Barcelona, Spain.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Planas
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology*; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods*; D004195:Disease Models, Animal; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D020244:Infarction, Middle Cerebral Artery / Q000175:diagnosis* / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D011336:Probability; D011379:Prognosis; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion
keywords: Apparent diffusion coefficient (ADC); Ischemic stroke; Magnetic resonance imaging (MRI); Stroke animal models