title: Effect of platelet-rich plasma therapy associated with exercise training in musculoskeletal healing in rats.
abstract: BACKGROUND
  Muscle healing is a time-dependent process associated with an increase in the total amount of local collagen fibers. Platelet-rich plasma therapy (PRPT) associated with exercise may improve this healing process. The aim of this study was to demonstrate the regenerative effect of PRPT in association with exercise training on musculoskeletal healing.
  
  
  METHODS
  Male Wistar rats were submitted to an injury in the vastus lateralis muscle and randomly divided into 4 groups (n = 5/group): sedentary sham-operated (SSO); sedentary group submitted to PRPT (SPR); swim-trained (SWT); and swim-trained group submitted to PRPT (SWP). Serum lactate level was used to confirm the training protocol effectiveness to increase aerobic fitness. The collagen fiber concentration was measured by the polarization colors in picrosirius red-stained tissue sections.
  
  
  RESULTS
  Lactate levels decreased in both training groups (SWT and SWP; P < .05) after training (SWT: from 6.2 ± 0.44 to 4.7 ± 0.22 mmol/L; SWP: from 5.5 ± 0.99 to 4.0 ± 0.78 mmol/L). There were less type 1 collagen fibers in SWP group compared with other groups (SSO = 31.8 ± 10.3, SSP = 32.3 ± 13.5, SWT = 14.6 ± 13.4, SWP = 5.7 ± 4.7, P < .05), while there were more type 3 collagen fibers on SWP (SSO = 68.7 ± 9.8, SSP = 71.2 ± 12.2, SWT = 85.4 ± 13.4, SWP = 94.4 ± 4.6, P < .05) in the injured region.
  
  
  CONCLUSIONS
  Exercise in association with PRPT enhances the skeletal muscle-healing process.
authors.affiliation: Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba, Paraná, Brazil; Universidade Positivo, Curitiba, Paraná, Brazil; Universidade Tecnológica Federal do Paraná, Curitiba, Paraná. Electronic address: ricardoccunha@gmail.com.
authors.forename: R C
authors.initials: RC
authors.lastname: Cunha
authors.affiliation: Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba, Paraná, Brazil; Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil; Universidade Tecnológica Federal do Paraná, Curitiba, Paraná
authors.forename: J C
authors.initials: JC
authors.lastname: Francisco
authors.affiliation: Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba, Paraná, Brazil.
authors.forename: M A
authors.initials: MA
authors.lastname: Cardoso
authors.affiliation: Universidade Positivo, Curitiba, Paraná, Brazil.
authors.forename: L F
authors.initials: LF
authors.lastname: Matos
authors.affiliation: Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Lino
authors.affiliation: Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil.
authors.forename: R B
authors.initials: RB
authors.lastname: Simeoni
authors.affiliation: Universidade Positivo, Curitiba, Paraná, Brazil; Universidade Federal do Paraná, Curitiba, Paraná, Brazil.
authors.forename: G
authors.initials: G
authors.lastname: Pereira
authors.affiliation: Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba, Paraná, Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: Irioda
authors.affiliation: Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil.
authors.forename: P R B
authors.initials: PR
authors.lastname: Simeoni
authors.affiliation: Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil.
authors.forename: L C
authors.initials: LC
authors.lastname: Guarita-Souza
authors.affiliation: Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba, Paraná, Brazil.
authors.forename: K A T
authors.initials: KA
authors.lastname: Carvalho
mesh_terms: D000818:Animals; D007773:Lactates / Q000097:blood; D008297:Male; D018482:Muscle, Skeletal / Q000293:injuries*; D010805:Physical Conditioning, Animal; D053657:Platelet-Rich Plasma; D017208:Rats, Wistar; D014945:Wound Healing / Q000502:physiology*
chemical_list: D007773:Lactates