title: KLK11 mRNA expression predicts poor disease-free and overall survival in colorectal adenocarcinoma patients.
abstract: BACKGROUND
  Dysregulated expression of several KLK family members has been observed in colorectal adenocarcinoma. In the present study, the prognostic value of KLK11 mRNA expression as a molecular tissue biomarker in colorectal adenocarcinoma was examined.
  
  
  METHODS
  Using quantitative real-time PCR, KLK11 mRNA expression was studied in 120 cancerous and 41 paired noncancerous colorectal specimens obtained from 120 patients with primary colorectal adenocarcinoma.
  
  
  RESULTS
  A significant upregulation of KLK11 transcripts in colorectal tumors was observed. KLK11 mRNA expression was associated with the depth of tumor invasion and the histological grade. Furthermore, KLK11 mRNA expression predicted poor disease-free and overall survival, independently of patient gender, age, tumor size, location, histological subtype, grade, venous invasion, lymphatic invasion, TNM stage, radiotherapy and chemotherapy treatment.
  
  
  CONCLUSIONS
  KLK11 mRNA expression could be considered as a new molecular prognostic biomarker in colorectal adenocarcinoma, with additional prognostic value in patients with highly invasive tumors and/or positive lymph nodes.
authors.affiliation: Department of Biochemistry & Molecular Biology, University of Athens, Panepistimiopolis, 15701, Athens, Greece.
authors.forename: Dimitra K
authors.initials: DK
authors.lastname: Alexopoulou
authors.forename: Christos K
authors.initials: CK
authors.lastname: Kontos
authors.forename: Spyridon
authors.initials: S
authors.lastname: Christodoulou
authors.forename: Iordanis N
authors.initials: IN
authors.lastname: Papadopoulos
authors.forename: Andreas
authors.initials: A
authors.lastname: Scorilas
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012697:Serine Endopeptidases / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; C114880:trypsin-like serine protease; D012697:Serine Endopeptidases
keywords: biological tumor markers; colon cancer; kallikreins; prognostic biomarkers; quantitative real-time PCR