title: Disuse deterioration of human skeletal muscle challenged by resistive exercise superimposed with vibration: evidence from structural and proteomic analysis.
abstract: In the present bed rest (BR) study, 23 volunteers were randomized into 3 subgroups: 60 d BR control (Ctr); BR with resistive exercise (RE; lower-limb load); and resistive vibration exercise (RVE; RE with superimposed vibration). The aim was to analyze by confocal and electron microscopy the effects of vibration on myofibril and filament integrity in soleus (Sol) and vastus lateralis (VL) muscle; differential proteomics of contractile, cytoskeletal, and costameric proteins (TN-C, ROCK1, and FAK); and expression of PGC1α and atrophy-related master genes MuRF1 and MuRF2. RVE (but not RE) preserved myofiber size and phenotype in Sol and VL by overexpressing MYBPC1 (42%, P ≤ 0.01), WDR1 (39%, P ≤ 0.01), sarcosin (84%, P ≤ 0.01), and CKM (20%, P ≤ 0.01) and prevented myofibrillar ultrastructural damage as detectable by MuRF1 expression. In Sol, cytoskeletal and contractile proteins were normalized by RVE, and TN-C increased (59%, P ≤ 0.01); the latter also with RE (108%, P ≤ 0.01). In VL, the outcomes of both RVE (acting on sarcosin and desmin) and RE (by way of troponinT-slow and MYL2) were similar. RVE appears to be a highly efficient countermeasure protocol against muscle atrophy and ultrastructural and molecular dysregulation induced by chronic disuse.
authors.affiliation: Center of Space Medicine Berlin, Neuromuscular Group, Institute of Anatomy, and michele.salanova@charite.de cecilia.gelfi@unimi.it.
authors.forename: Michele
authors.initials: M
authors.lastname: Salanova
authors.affiliation: Department of Biomedical Sciences for Health, University of Milan, Milan, Italy; Consiglio Nazionale delle Ricerche (CNR), Institute of Bioimaging and Molecular Physiology, Segrate, Italy; Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Policlinico San Donato, San Donato Milanese, Italy; michele.salanova@charite.de cecilia.gelfi@unimi.it.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Gelfi
authors.affiliation: Department of Biomedical Sciences for Health, University of Milan, Milan, Italy;
authors.forename: Manuela
authors.initials: M
authors.lastname: Moriggi
authors.affiliation: Consiglio Nazionale delle Ricerche (CNR), Institute of Bioimaging and Molecular Physiology, Segrate, Italy; Institute of Bioimaging and Molecular Physiology, CNR-Laboratorio di Tecnologie Oncologiche (LATO), Cefalù, Italy; and.
authors.forename: Michele
authors.initials: M
authors.lastname: Vasso
authors.affiliation: Department of Biomedical Sciences for Health, University of Milan, Milan, Italy;
authors.forename: Agnese
authors.initials: A
authors.lastname: Viganò
authors.affiliation: Institute of Bioimaging and Molecular Physiology, CNR-Laboratorio di Tecnologie Oncologiche (LATO), Cefalù, Italy; and.
authors.forename: Luigi
authors.initials: L
authors.lastname: Minafra
authors.affiliation: Department of Pharmacology and Toxicology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Gaetano
authors.initials: G
authors.lastname: Bonifacio
authors.affiliation: Center of Space Medicine Berlin, Neuromuscular Group, Institute of Anatomy, and.
authors.forename: Gudrun
authors.initials: G
authors.lastname: Schiffl
authors.affiliation: Center of Space Medicine Berlin, Neuromuscular Group, Institute of Anatomy, and.
authors.forename: Martina
authors.initials: M
authors.lastname: Gutsmann
authors.affiliation: Center for Muscle and Bone Research (ZMK), Charité Universitätsmedizin Berlin, Berlin, Germany;
authors.forename: Dieter
authors.initials: D
authors.lastname: Felsenberg
authors.affiliation: Consiglio Nazionale delle Ricerche (CNR), Institute of Bioimaging and Molecular Physiology, Segrate, Italy;
authors.forename: Paolo
authors.initials: P
authors.lastname: Cerretelli
authors.affiliation: Center of Space Medicine Berlin, Neuromuscular Group, Institute of Anatomy, and.
authors.forename: Dieter
authors.initials: D
authors.lastname: Blottner
mesh_terms: D000328:Adult; D001510:Bed Rest / Q000379:methods; D005081:Exercise Therapy; D006801:Humans; D008297:Male; D008875:Middle Aged; D009119:Muscle Contraction / Q000502:physiology; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D009133:Muscular Atrophy / Q000378:metabolism* / Q000628:therapy; D040901:Proteomics; D014732:Vibration; D055815:Young Adult
keywords: atrophy; bed rest; countermeasures; protein expression; ultrastructure