title: Quercetin reduces pluripotency, migration and adhesion of human teratocarcinoma cell line NT2/D1 by inhibiting Wnt/β-catenin signaling.
abstract: Quercetin, a bioflavonoid found in plant foods, has a wide range of therapeutic effects. In order to examine the therapeutic potential of quercetin in teratocarcinoma, we used the human teratocarcinoma cell line NT2/D1 as an in vitro model. We have shown that quercetin inhibits the proliferation, adhesion and migration of NT2/D1 cells and downregulates the expression of pluripotency factors SOX2, Oct4 and Nanog. Our results further suggest that the anticancer effect of quercetin against human teratocarcinoma cells is mediated by targeting the canonical Wnt signaling pathway. Quercetin antagonized the Wnt/β-catenin signaling pathway in NT2/D1 cells by inhibiting β-catenin nuclear translocation and the consequent downregulation of β-catenin-dependent transcription. These data suggest that quercetin as a potent inhibitor of Wnt signaling may be an effective therapeutic agent in cancers with aberrant activation of the Wnt pathway.
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11010 Belgrade, Serbia. mojsin@imgge.bg.ac.rs.
authors.forename: Marija
authors.initials: M
authors.lastname: Mojsin
authors.forename: Jelena Marjanovic
authors.initials: JM
authors.lastname: Vicentic
authors.forename: Marija
authors.initials: M
authors.lastname: Schwirtlich
authors.forename: Vladanka
authors.initials: V
authors.lastname: Topalovic
authors.forename: Milena
authors.initials: M
authors.lastname: Stevanovic
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation; D018398:Homeodomain Proteins / Q000378:metabolism; D006801:Humans; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D018243:Teratocarcinoma / Q000378:metabolism; D014162:Transfection; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: C495270:CTNNB1 protein, human; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D051176:beta Catenin; D011794:Quercetin