title: Identification and quantification of antitumor thioproline and methylthioproline in Korean traditional foods by a liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry.
abstract: A liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometric method (LC-APCI-MS/MS) has been developed for the sensitive determination of antitumor thioproline and methylthioproline from fermented foods. Thioproline and methylthioproline were derivatized in one step with ethyl chloroformate at room temperature. These compounds were identified and quantified in various traditional Korean fermented foods by LC-APCI-MS/MS. The concentration range of thioproline of each food was found for doenjang (0.011-0.032mg/kg), gochujang (0.010-0.038mg/kg), and ganjang (0.010-0.038mg/kg). Those of methylthioproline of each food was found for doenjang (0.098-0.632mg/kg), gochujang (0.015-0.112mg/kg), and ganjang (0.023-1.468mg/kg). A prolonged aging time leads to an increase in both the thioproline and methylthioproline contents, suggesting that the storage time plays a key role in the formation of thioproline and methylthioproline in Korean traditional foods. The results here suggest that thioproline and methylthioproline are related to the biological activities of traditional Korean fermented foods.
authors.affiliation: Department of Technology & Home Economics Education, Kongju National University, Kongju 314-701, Republic of Korea.
authors.forename: Sun Hyo
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Environmental Science, Kongju National University, Kongju 314-701, Republic of Korea.
authors.forename: Hyun-Ji
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Environmental Education, Kongju National University, Kongju 314-701, Republic of Korea. Electronic address: hshin@kongju.ac.kr.
authors.forename: Ho-Sang
authors.initials: HS
authors.lastname: Shin
mesh_terms: D016588:Anticarcinogenic Agents / Q000032:analysis*; D044466:Asian People; D001274:Atmospheric Pressure; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D005247:Feeding Behavior / Q000208:ethnology; D005285:Fermentation; D005504:Food Analysis / Q000379:methods* / Q000592:standards; D006801:Humans; D008745:Methylation; D012015:Reference Standards; D015203:Reproducibility of Results; D056910:Republic of Korea; D045730:Soy Foods / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000592:standards; D053719:Tandem Mass Spectrometry / Q000592:standards; D053778:Thiazolidines / Q000032:analysis*; D013997:Time Factors
chemical_list: D016588:Anticarcinogenic Agents; D053778:Thiazolidines; C003438:thiazolidine-4-carboxylic acid
keywords: Identification; Liquid chromatography–mass spectrometry; Methylthioproline; Thioproline; Traditional Korean fermented foods