title: Analysis of isoamylase debranched starches with size exclusion chromatography utilizing PFG columns.
abstract: Debranched starches were tested with a previously developed method for size exclusion chromatography (SEC) with multi detection utilizing different columns than usually used for the separation of starch in DMSO. A number of debranched starches were analyzed. This system allows good separation of amylose and amylopectin after debranching of starch, and provides quantitative information on the amylose content. Additionally molar mass versus hydrodynamic radii (Rh) distributions of various debranched starches show that the debranching was not 100% and that the differences in the structure of various starches can be followed.
authors.affiliation: Department of Polymer Chemistry & Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands; Dutch Polymer Institute (DPI), P.O. Box 902, 5600 AX Eindhoven, The Netherlands. Electronic address: jelenaciricnl@gmail.com.
authors.forename: Jelena
authors.initials: J
authors.lastname: Ciric
authors.affiliation: Department of Polymer Chemistry & Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands. Electronic address: a.j.j.woortman@rug.nl.
authors.forename: Albert J J
authors.initials: AJ
authors.lastname: Woortman
authors.affiliation: Department of Polymer Chemistry & Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands; Dutch Polymer Institute (DPI), P.O. Box 902, 5600 AX Eindhoven, The Netherlands. Electronic address: k.u.loos@rug.nl.
authors.forename: Katja
authors.initials: K
authors.lastname: Loos
keywords: Chain-length distribution; Debranching; SEC; Starch