title: [Preparation and property study of doxorubicin loaded microspheres].
abstract: OBJECTIVE
  To prepare doxorubicin-loaded polyvinylalcohol-acrylic acid (PVA-AA) microspheres and evaluate properties of this chemoembolic agent.
  
  
  METHODS
  PVA-AA microspheres were synthesized by inverse suspension polymerization method and then verified by infrared spectroscopy. drug loading (DL) and entrapment efficiency (EE%) were measured after doxorubicinwas loaded on PVA-AA microspheres. Their morphology and elasticity were investigated by optical microscope, environmental scanning electron microscope and texture analyzer, respectively. T-cell apparatus was used to evaluate the in vitro release behavior of doxorubicin-loaded microspheres.The external carotid of the rabbit was chosen as an embolization site to evaluate the in vivo embolic property of the microspheres.
  
  
  RESULTS
  PVA-AA microspheres, which were transparent spheres,turned into red spheres after doxorubicin loading. DL of the microspheres was (20.56 ± 0.69)g/L and (23.25 ± 0.27) g/L,and EE% was 82.22% ± 2.76% and 93.00% ± 1.06% within 20 min and 6 h, respectively. The in vitro release results showed a significantly delayed release of the drug for 10.32% ± 0.47% after 24 h. The Young's modulus was (178.30 ± 12.33) kPa and (213.29 ± 15.61) kPa for blank microspheres and doxorubicin-loaded microspheres, respectively. Both blank microspheres and doxorubicin-loaded microspheres exhibited good elasticity. In vivo embolization showed that 0.3 mL of microspheres could produce distal embolic efficiency.
  
  
  CONCLUSIONS
  The doxorubicin-loaded microspheres are expected to be a promising new chemoembolic agent.
authors.affiliation: Department of Pharmaceutics,Peking University School of Pharmaceutical Sciences,Beijing 100191,China.
authors.forename: Er-a
authors.initials: EA
authors.lastname: Sa
authors.affiliation: Department of Pharmaceutics,Peking University School of Pharmaceutical Sciences,Beijing 100191,China.
authors.forename: Xiao-jing
authors.initials: XJ
authors.lastname: Lu
authors.affiliation: Department of Pharmaceutics,Peking University School of Pharmaceutical Sciences,Beijing 100191,China.
authors.forename: Dai-chao
authors.initials: DC
authors.lastname: Cui
authors.affiliation: Department of Interventional Radiology and Vascular Surgery,Peking University First Hospital,Beijing 100034,China.
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Guan
authors.affiliation: Department of Interventional Radiology and Vascular Surgery,Peking University First Hospital,Beijing 100034,China.
authors.forename: Tian-shi
authors.initials: TS
authors.lastname: Lv
authors.affiliation: Department of Interventional Radiology and Vascular Surgery,Peking University First Hospital,Beijing 100034,China.
authors.forename: Shei-sheng
authors.initials: SS
authors.lastname: Zhang
authors.affiliation: Department of Interventional Radiology and Vascular Surgery,Peking University First Hospital,Beijing 100034,China.
authors.forename: Zi-guang
authors.initials: ZG
authors.lastname: Yan
authors.affiliation: Department of Interventional Radiology and Vascular Surgery,Peking University First Hospital,Beijing 100034,China.
authors.forename: Li
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of Interventional Radiology and Vascular Surgery,Peking University First Hospital,Beijing 100034,China.
authors.forename: Ying-hua
authors.initials: YH
authors.lastname: Zou
authors.affiliation: Department of Pharmaceutics,Peking University School of Pharmaceutical Sciences,Beijing 100191,China.
authors.forename: Tian-yuan
authors.initials: TY
authors.lastname: Fan
mesh_terms: D000179:Acrylates; D000818:Animals; D004317:Doxorubicin / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D004548:Elasticity; D004621:Embolization, Therapeutic; D008863:Microspheres; D011142:Polyvinyl Alcohol; D011817:Rabbits
chemical_list: D000179:Acrylates; D004337:Drug Carriers; D004317:Doxorubicin; D011142:Polyvinyl Alcohol; C036658:acrylic acid