title: In vitro evaluation of the inhibitory effect of canine serum, canine fresh frozen plasma, freeze-thaw-cycled plasma, and Solcoseryl™ on matrix metalloproteinases 2 and 9.
abstract: OBJECTIVE
  Compare the efficacy of canine serum, fresh frozen plasma (FFP), freeze-thaw-cycled plasma (FTCP), and Solcoseryl(™) at inhibiting matrix metalloproteinases (MMP) 2 and 9 in vitro.
  
  
  METHODS
  Matrix metalloproteinases 2 and 9 activity in the presence of serum, FFP, FTCP, or Solcoseryl(™) was assayed using a commercially available fluorogenic gelatinase activity kit.
  
  
  RESULTS
  Matrix metalloproteinases 2 activity in the presence of serum, FFP, FTCP, and Solcoseryl(™) was 20.84%, 5.76%, 8.10%, and 83.03%, respectively of uninhibited MMP 2 activity. MMP 9 activity in the presence of serum, FFP, FTCP, and Solcoseryl(™) was 57.36%, 58.35%, 49.35%, and -8.69%, respectively of uninhibited MMP 9 activity.
  
  
  CONCLUSIONS
  Serum, FFP, and FTCP exhibit similar levels of MMP 2 and 9 inhibitions. Solcoseryl(™) causes minimal MMP 2 inhibition, but profound MMP 9 inhibition.
authors.affiliation: Peace Avenue Veterinary Clinic, G/F 7B Liberty Road, Homantin, Kowloon, Hong Kong.
authors.forename: Derek W Y
authors.initials: DW
authors.lastname: Chow
authors.forename: Ying
authors.initials: Y
authors.lastname: Chau
authors.forename: Wai Kit
authors.initials: WK
authors.lastname: Yeung
authors.forename: Hans D
authors.initials: HD
authors.lastname: Westermeyer
mesh_terms: D000183:Actihaemyl; D000818:Animals; D004285:Dogs; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D061965:Matrix Metalloproteinase Inhibitors / Q000494:pharmacology*; D010949:Plasma; D044967:Serum
chemical_list: D061965:Matrix Metalloproteinase Inhibitors; D000183:Actihaemyl; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9
keywords: Solcoseryl™; hemodialysate; keratomalacia; matrix metalloproteinases; plasma; serum