title: Detection of the Helicobacter pylori dupA gene is strongly affected by the PCR design.
abstract: The Helicobacter pylori virulence gene dupA is usually detected by PCR, but the primer binding sites used are highly variable. Our newly designed qPCR against a conserved region of dupA was positive in 64.2% of 394 clinical isolates while the positivity rate of the commonly used PCRs ranged from 29.9% to 37.8%.
authors.affiliation: Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Amin Talebi Bezmin
authors.initials: ATB
authors.lastname: Abadi
authors.affiliation: Zaandam Department of Internal Medicine, Zaans Medical Center, Zaandam, The Netherlands.
authors.forename: Ruud J L F
authors.initials: RJLF
authors.lastname: Loffeld
authors.affiliation: Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Ashandra C
authors.initials: AC
authors.lastname: Constancia
authors.affiliation: Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht, Utrecht, The Netherlands.
authors.forename: Jaap A
authors.initials: JA
authors.lastname: Wagenaar
authors.affiliation: Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands. Electronic address: h.kusters@umcutrecht.nl.
authors.forename: Johannes G
authors.initials: JG
authors.lastname: Kusters
mesh_terms: D017931:DNA Primers / Q000235:genetics; D016480:Helicobacter pylori / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D017931:DNA Primers; C528428:DupA protein, Helicobacter pylori; D037521:Virulence Factors
keywords: Helicobacter pylori; Primer sets; dupA