title: Spectroscopic and permeation studies of phospholipid bilayers supported by a soft hydrogel scaffold.
abstract: Polarized attenuated total reflection infrared (ATR-IR) spectroscopy, fluorescence microscopy, and fluorescence spectroscopy were used to characterize a lipid coating composed of 70 mol % 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 30 mol % cholesterol, supported on a spherical hydrogel scaffold. The fluorescence microscopy images show an association between the lipid coating and the hydrogel scaffold. Fluorescence permeability measurements revealed that the phospholipid coating acts as a permeability barrier, exhibiting characteristics of a lamellar bilayer coating structure. Variable evanescent wave penetration depth ATR-IR spectroscopy studies validated the determination of quantitative molecular orientation information for a lipid coating supported on a spherical scaffold. These polarized ATR-IR studies measured an average DMPC acyl chain tilt angle of ∼21-25°, with respect to the surface normal.
authors.affiliation: Department of Chemistry, University of Guelph , Guelph, Ontario N1G 2W1, Canada.
authors.forename: Michael
authors.initials: M
authors.lastname: Grossutti
authors.forename: Ryan
authors.initials: R
authors.lastname: Seenath
authors.forename: Shannon
authors.initials: S
authors.lastname: Conlon
authors.forename: J Jay
authors.initials: JJ
authors.lastname: Leitch
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Jacek
authors.initials: J
authors.lastname: Lipkowski
mesh_terms: D002784:Cholesterol / Q000138:chemical synthesis / Q000737:chemistry; D004134:Dimyristoylphosphatidylcholine / Q000138:chemical synthesis / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008051:Lipid Bilayers / Q000138:chemical synthesis / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D013055:Spectrophotometry, Infrared
chemical_list: D008051:Lipid Bilayers; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D002784:Cholesterol; D004134:Dimyristoylphosphatidylcholine