title: Attempts to increase inosinic acid in broiler meat by using feed additives.
abstract: To explore regulation of inosinic acid content in chicken meat as a result of feed additives, 576 one-day-old male Arbor Acres broilers were randomly allotted into 8 dietary treatments including control, purine nucleotide (P), betaine (B), soybean isoflavone (S), purine nucleotide + betaine (PB), purine nucleotide + soybean isoflavone (PS), betaine +soybean isoflavone (BS), and purine nucleotide + betaine + soybean isoflavone (PBS) by a 2 × 2 × 2 factorial arrangement. At d 42 of age, broilers were slaughtered, and growth performance, carcass characteristics, inosinic acid content, and activities of enzyme closely related to inosinic acid metabolism of broilers were measured. The results revealed that these feed additives did not affect ADG and ADFI of the broilers (P > 0.05). However, supplementing purine nucleotides lowered feed/gain of broilers in PS and PBS groups (P < 0.05). There was a significant interaction on feed/gain of broilers between purine nucleotides and soybean isoflavone (P < 0.05). The abdominal fat percentages in groups B, S, BS, and PBS were lower than the control group, respectively (P < 0.05). The thigh muscle percentages of groups P and B were higher than that of group PB (P < 0.05). There were certain interactions on the percentage of thigh muscle (P = 0.05) and abdominal fat (P < 0.05) between P, B, and S groups. Compared with the control group, inosinic acid content in broiler breast meat was improved by using feed additives (P < 0.05). Supplementing purine nucleotides, betaine, soybean isoflavone, and their combinations increased alkaline phosphatase activity in breast meat of broilers (P < 0.05). Purine nucleotides improved the activity of adenosine deaminase, but decreased the activity of 5'-nucleotidase. Soybean isoflavone lowered the activity of alkaline phosphatase. There were no significant interactions on activities of creatine kinase, adenosine deaminase, alkaline phosphatase, and 5'-nucleotidase between these additives (P > 0.05). The umami rating of broiler breast meat increased in conjunction with supplementing these additives. In conclusion, supplementing standard feed with the additives investigated in this study could improve inosinic acid content in chicken meat by increasing synthase activity or inhibiting degradation enzyme activity without inferior growth performance and carcass quality.
authors.affiliation: The Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, China.
authors.forename: G H
authors.initials: GH
authors.lastname: Liu
authors.affiliation: The Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Cai
authors.affiliation: The Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, China changwenhuan@caas.cn.
authors.forename: W H
authors.initials: WH
authors.lastname: Chang
authors.affiliation: The Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, China.
authors.forename: J S
authors.initials: JS
authors.lastname: Ma
authors.affiliation: The Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, China.
authors.forename: A J
authors.initials: AJ
authors.lastname: Zheng
authors.affiliation: The Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, China.
authors.forename: S
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001622:Betaine / Q000008:administration & dosage / Q000378:metabolism*; D002645:Chickens / Q000254:growth & development / Q000502:physiology*; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements / Q000032:analysis; D007291:Inosine Monophosphate / Q000378:metabolism*; D007529:Isoflavones / Q000008:administration & dosage / Q000378:metabolism*; D008297:Male; D008460:Meat / Q000032:analysis; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000201:enzymology / Q000502:physiology; D011685:Purine Nucleotides / Q000008:administration & dosage / Q000378:metabolism*; D011897:Random Allocation; D013025:Glycine max / Q000737:chemistry
chemical_list: D007529:Isoflavones; D011685:Purine Nucleotides; D007291:Inosine Monophosphate; D001622:Betaine
keywords: broiler meat; enzyme activity; feed additive; inosinic acid