title: The Iho670 fibers of Ignicoccus hospitalis are anchored in the cell by a spherical structure located beneath the inner membrane.
abstract: The Iho670 fibers of the hyperthermophilic crenarchaeon of Ignicoccus hospitalis were shown to contain several features that indicate them as type IV pilus-like structures. The application of different visualization methods, including electron tomography and the reconstruction of a three-dimensional model, enabled a detailed description of a hitherto undescribed anchoring structure of the cell appendages. It could be identified as a spherical structure beneath the inner membrane. Furthermore, pools of the fiber protein Iho670 could be localized in the inner as well as the outer cellular membrane of I. hospitalis cells and in the tubes/vesicles in the intermembrane compartment by immunological methods.
authors.affiliation: Institute for Microbiology, University of Regensburg, Regensburg, Germany.
authors.forename: Carolin
authors.initials: C
authors.lastname: Meyer
authors.affiliation: Institute for Microbiology, University of Regensburg, Regensburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Heimerl
authors.affiliation: Institute for Microbiology, University of Regensburg, Regensburg, Germany.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Wirth
authors.affiliation: LOEWE Center for Synthetic Microbiology (Synmikro) and Department of Cell Biology, Philipps-Universität Marburg, Marburg, Germany Plant Development, Biocenter of the LMU Munich, Planegg-Martinsried, Germany andreas.klingl@biologie.uni-muenchen.de reinhard.rachel@biologie.uni-regensburg.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Klingl
authors.affiliation: Institute for Microbiology, University of Regensburg, Regensburg, Germany andreas.klingl@biologie.uni-muenchen.de reinhard.rachel@biologie.uni-regensburg.de.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Rachel
mesh_terms: D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000502:physiology*; D019620:Desulfurococcaceae / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D019848:Gene Expression Regulation, Archaeal / Q000502:physiology*; D007150:Immunohistochemistry; D009068:Movement; D011487:Protein Conformation
chemical_list: D019843:Archaeal Proteins