title: Monoclonal antibody-based immunoassay for analysis of octopamine in housefly.
abstract: Octopamine (OA) is one of the biogenic monoamines in the housefly, which acts as an important neurohormone in the physiological process of this pest. In this study, a new hapten of OA was synthesized via aldol condensation. With the hapten, monoclonal antibodies (MAb) were generated and their characterizations were investigated. An indirect competitive enzyme-linked immunosorbent assay (icELISA) based on MAb 3C11-E3 was established, which required simple sample pre-treatments and had low cross-reactivity with OA structural analogise. The half maximal inhibition concentration (IC50) and the detected range (IC20-IC80) of the icELISA were 128 ng/mL and 12-1438 ng/mL, respectively. Average recoveries of OA ranged from 73 to 129% in the housefly.
authors.affiliation: College of Agronomy and Biotechnology, China Agricultural University , Beijing, China .
authors.forename: Yujing
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Cao
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Yongdan
authors.initials: Y
authors.lastname: Li
authors.forename: Guiyu
authors.initials: G
authors.lastname: Tan
authors.forename: Baomin
authors.initials: B
authors.lastname: Wang
authors.forename: Xiwu
authors.initials: X
authors.lastname: Gao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D006241:Haptens / Q000096:biosynthesis / Q000276:immunology; D006793:Houseflies / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D009655:Octopamine / Q000032:analysis* / Q000737:chemistry / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D006241:Haptens; D009655:Octopamine