title: T cell isolation from mouse kidneys.
abstract: The kidneys contain very few lymphocytes under homeostatic conditions. One kidney from a healthy mouse per average contains only 1-5 × 10(3) CD4(+) T cells. In immune-mediated kidney disease, γδ T cells, NKT cells, CD4(+) T cells, CD8(+) T cells, and regulatory T cells (Treg) infiltrate the kidney. Their numbers and subset composition of infiltrating T cells varies between the different forms of nephritis. For example, in glomerulonephritis CD4(+) T cells mediate renal injury, by local cytokine production, effector cell activation and/or by helping B cells to produce nephritogenic antibodies. A better understanding of the pathomechanisms of immune-mediated kidney diseases requires a method to isolate T cells from the kidney for ex vivo analysis. Here we describe an effective and specific isolation protocol for T cells from the murine kidney.
authors.affiliation: Institute of Experimental Immunology, Rheinische Friedrich-Wilhelms-University of Bonn, Sigmund-Freud-Str. 25, 53105, Bonn, Germany.
authors.forename: Isis
authors.initials: I
authors.lastname: Ludwig-Portugall
authors.forename: Christian
authors.initials: C
authors.lastname: Kurts
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D018189:Immunomagnetic Separation / Q000379:methods; D007668:Kidney / Q000166:cytology* / Q000276:immunology*; D051379:Mice; D010477:Perfusion / Q000379:methods; D013601:T-Lymphocytes / Q000166:cytology*