title: Intracellular staining for cytokines and transcription factors.
abstract: Within the past years immune cells in general and T cells in particular have been categorized into a vast variety of subsets with different functional properties. One of the key technologies fueling this emerging complexity is intracellular staining for effector cytokines and/or lineage-defining transcription factors. Here we discuss the critical steps for performing successful multicolor immunophenotyping of mouse T cells in combination with analysis of intracellular molecules after ex vivo isolation.
authors.affiliation: Institute of Experimental Immunology, University of Zürich, Winterthurerstrasse 190, 8057, Zürich, Switzerland, mair@immunology.uzh.ch.
authors.forename: Florian
authors.initials: F
authors.lastname: Mair
authors.forename: Vinko
authors.initials: V
authors.lastname: Tosevski
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002469:Cell Separation / Q000379:methods; D016207:Cytokines / Q000378:metabolism*; D007424:Intracellular Fluid / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D051379:Mice; D013194:Staining and Labeling / Q000379:methods*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D016207:Cytokines; D014157:Transcription Factors