title: A genetic strategy to obtain P-Gal4 elements in the Drosophila Hox genes.
abstract: The Drosophila Gal4/UAS system allows the expression of any gene of interest in restricted domains. We devised a genetic strategy, based on the P-element replacement and UAS-y (+) techniques, to generate Gal4 lines inserted in Hox genes of Drosophila that are, at the same time, mutant for the resident genes. This makes possible to express different wild-type or mutant Hox proteins in the precise domains of Hox gene expression, and thus to test the functional value of these proteins in mutant rescue experiments.
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, C/Nicolás Cabrera 1, Cantoblanco, 28049, Madrid, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: de Navas
authors.forename: David
authors.initials: D
authors.lastname: Foronda
authors.forename: Delia
authors.initials: D
authors.lastname: Del Saz
authors.forename: Ernesto
authors.initials: E
authors.lastname: Sánchez-Herrero
mesh_terms: D000818:Animals; D003433:Crosses, Genetic; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics*; D008297:Male; D050436:Regulatory Elements, Transcriptional; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; C557617:GAL4 protein, Drosophila; D018398:Homeodomain Proteins; D014157:Transcription Factors