title: Analysis and behavior of colistin during anaerobic fermentation.
abstract: A new analytical method for the determination of colistin in fermenter samples was developed followed by a study on the behavior of this substance during anaerobic fermentation. Analysis of colistin A and B was carried out by liquid chromatography-tandem mass spectrometry. Separation of the analytes was performed on a Security Guard column (4×3mm). Fourteen fermentation tests in batch as well as in continuous reactors were carried out. After 44days of anaerobic digestion of cattle manure, initially spiked with 500mg/kg of colistin sulfate, a considerable decrease of the colistin concentration to less than 1mg/kg could be observed. Furthermore, the daily production of biogas and methane was measured. A correlation between gas production and colistin concentration could not be determined. However, an increase of 10% of the cumulative methane production was observed in those fermenters spiked with an initial bolus of 500mg/kg colistin.
authors.affiliation: Landesbetrieb Hessisches Landeslabor (LHL), Standort Kassel, Druseltalstraße 67, 34131 Kassel, Germany; Justus Liebig University Giessen, Institute of Food Chemistry and Food Biotechnology, Heinrich-Buff-Ring 58, 35392 Giessen, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Riemenschneider
authors.affiliation: LHL, Standort Bad Hersfeld, Schloss Eichhof, 36251 Bad Hersfeld, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Zerr
authors.affiliation: Landesbetrieb Hessisches Landeslabor (LHL), Standort Kassel, Druseltalstraße 67, 34131 Kassel, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Vater
authors.affiliation: LHL, Standort Giessen, Schubertstraße 60, 35392 Giessen, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Brunn
authors.affiliation: Justus Liebig University Giessen, Institute of Food Chemistry and Food Biotechnology, Heinrich-Buff-Ring 58, 35392 Giessen, Germany.
authors.forename: S A I
authors.initials: SAI
authors.lastname: Mohring
authors.affiliation: Justus Liebig University Giessen, Institute of Food Chemistry and Food Biotechnology, Heinrich-Buff-Ring 58, 35392 Giessen, Germany. Electronic address: Gerd.Hamscher@lcb.chemie.uni-giessen.de.
authors.forename: G
authors.initials: G
authors.lastname: Hamscher
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003091:Colistin / Q000032:analysis / Q000737:chemistry*; D005285:Fermentation / Q000502:physiology*; D008372:Manure / Q000032:analysis*; D008697:Methane / Q000096:biosynthesis*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
chemical_list: D008372:Manure; D008697:Methane; D003091:Colistin
keywords: Anaerobic digestion; Colistin; Fermented manure; LC–MS/MS; Methane