title: Antineutrophil cytoplasmic antibody frequency in chronic hepatitis B patients.
abstract: BACKGROUND
  Chronic hepatitis B (CHB) is a viral disease, common across the world, and associated with several extraintestinal manifestations including vasculitis. Antineutrophil cytoplasmic antibodies (ANCAs) are sensitive and specific markers for vasculitides. There is limited data available in the literature on whether ANCA formation is stimulated by CHB infection. In the present study we aimed to identify the frequency of ANCA in CHB patients.
  
  
  METHODS
  A total of 174 subjects were included in the study (87 CHB patients, 87 control subjects). Perinuclear-ANCA (P-ANCA), cytoplasmic-ANCA (C-ANCA), myeloperoxidase ANCA (MPO-ANCA), and proteinase 3-ANCA (PR3-ANCA) were studied. IFA was used for P-ANCA and C-ANCA assays, and ELISA was used for MPO-ANCA and PR3-ANCA assays.
  
  
  RESULTS
  ANCA positivity was high in both groups (31% in the CHB group and 26% among controls). There were no significant differences between the groups for P-ANCA and MPO-ANCA (P = 0.6 and P = 0.6, resp.). Frequency of borderline positive C-ANCA and all positive PR3-ANCA (positive + borderline positive) was significantly higher in the CHB group (P = 0.009 and P = 0.005, resp.).
  
  
  CONCLUSIONS
  In the present study, the frequency of ANCA was high in both groups. The CHB group had a relatively higher frequency of ANCA positivity compared to controls. Borderline positive C-ANCA and positive PR3-ANCA were significantly higher in the CHB group. These results suggest that ANCA may have a high prevalence in Turkey. Patients with CHB should be evaluated particularly for C-ANCA and PR3-ANCA in the presence of vasculitic complaints and lesions.
authors.affiliation: Department of Gastroenterology, Umraniye Education and Research Hospital, 34764 Istanbul, Turkey.
authors.forename: Turan
authors.initials: T
authors.lastname: Calhan
authors.affiliation: Department of Gastroenterology, Umraniye Education and Research Hospital, 34764 Istanbul, Turkey.
authors.forename: Abdurrahman
authors.identifier: 0000-0001-5477-9492
authors.initials: A
authors.lastname: Sahin
authors.affiliation: Department of Gastroenterology, Umraniye Education and Research Hospital, 34764 Istanbul, Turkey.
authors.forename: Resul
authors.initials: R
authors.lastname: Kahraman
authors.affiliation: Department of Gastroenterology, Haydarpasa Numune Education and Research Hospital, Istanbul, Turkey.
authors.forename: Mustafa Erhan
authors.initials: ME
authors.lastname: Altunoz
authors.affiliation: Central Research Laboratory, Cerrahpasa Medical Faculty, University of Istanbul, Istanbul, Turkey.
authors.forename: Fatma
authors.initials: F
authors.lastname: Ozbakır
authors.affiliation: Department of Gastroenterology, Umraniye Education and Research Hospital, 34764 Istanbul, Turkey.
authors.forename: Kamil
authors.initials: K
authors.lastname: Ozdil
authors.affiliation: Department of Gastroenterology, Umraniye Education and Research Hospital, 34764 Istanbul, Turkey.
authors.forename: Hacı Mehmet
authors.initials: HM
authors.lastname: Sokmen
mesh_terms: D000328:Adult; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D019694:Hepatitis B, Chronic / Q000097:blood / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic