title: Adipose-derived stem cells: methods for isolation and applications for clinical use.
abstract: Adipose tissue sciences have rapidly expanded since the identification of regenerative cells contained within the stromal vascular fraction (SVF) of fat. Isolation of the SVF, containing adipose-derived stem cells (ADSC), can be accomplished efficiently in the operating room or in the laboratory through enzymatic digestion of the adipose tissue and concentration of SVF. Cells can be directly re-injected as a mesotherapeutic agent, recombined with a tissue scaffold (e.g., cell-enriched fat grafts) or expanded in culture for tissue-engineered cell therapeutics. The potential for cell therapy is under current investigation by researchers around the world. This chapter reviews laboratory methods for isolating ADSCs and the ongoing clinical trials evaluating cell therapeutic efficacy across many specialties, including cardiology, neurology, immunology, tissue engineering, sports medicine, and plastic and reconstructive surgery.
authors.affiliation: Department of Surgery, University of California San Diego, San Diego, CA, USA, scohen@facesplus.com.
authors.forename: Brian
authors.initials: B
authors.lastname: Mailey
authors.forename: Ava
authors.initials: A
authors.lastname: Hosseini
authors.forename: Jennifer
authors.initials: J
authors.lastname: Baker
authors.forename: Adam
authors.initials: A
authors.lastname: Young
authors.forename: Zeni
authors.initials: Z
authors.lastname: Alfonso
authors.forename: Kevin
authors.initials: K
authors.lastname: Hicok
authors.forename: Anne M
authors.initials: AM
authors.lastname: Wallace
authors.forename: Steven R
authors.initials: SR
authors.lastname: Cohen
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D003114:Colony-Forming Units Assay; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D044968:Regenerative Medicine; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering