title: Regenerative potential of low-concentration SDS-decellularized porcine aortic valved conduits in vivo.
abstract: The aims of this study were to determine the functional biocompatibility of low-concentration SDS-decellularized porcine aortic roots in vivo. A previously developed process was modified for 9- and 15-mm-diameter aortic roots to facilitate implantation into the porcine abdominal aorta (n=3) and juvenile sheep right ventricular outflow tract (n=7), respectively. Native allogeneic aortic roots were used as controls. Acellular porcine roots explanted from pigs at weeks were largely repopulated with stromal cells of appropriate phenotype, and there was evidence that macrophages were involved in the regenerative process. Native allogeneic roots were subject to a classic allograft rejection response. Acellular porcine roots explanted from sheep at 6 months showed evidence of appropriate cellular repopulation, again with evidence of a role for macrophages in the regenerative process. There was some degree of calcification of two of the explanted acellular roots, likely due to incomplete removal of DNA before implantation. Native allogeneic ovine roots were subject to a classic allograft rejection response involving T cells, which resulted in overtly calcified and damaged tissues. The study highlighted (1) the importance of removal of DNA from acellular porcine valved roots to avoid calcification and (2) a role for macrophages in the regeneration of low-concentration SDS-decellularized aortic roots, as has been reported for other acellular biological extracellular matrix scaffolds.
authors.affiliation: 1 Faculty of Biological Sciences, School of Biomedical Sciences, University of Leeds , Leeds, United Kingdom .
authors.forename: José Rodolfo
authors.initials: JR
authors.lastname: Paniagua Gutierrez
authors.forename: Helen
authors.initials: H
authors.lastname: Berry
authors.forename: Sotirios
authors.initials: S
authors.lastname: Korossis
authors.forename: Saeed
authors.initials: S
authors.lastname: Mirsadraee
authors.forename: Sergio Veiga
authors.initials: SV
authors.lastname: Lopes
authors.forename: Francisco
authors.initials: F
authors.lastname: da Costa
authors.forename: John
authors.initials: J
authors.lastname: Kearney
authors.forename: Kevin
authors.initials: K
authors.lastname: Watterson
authors.forename: John
authors.initials: J
authors.lastname: Fisher
authors.forename: Eileen
authors.initials: E
authors.lastname: Ingham
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology; D001021:Aortic Valve / Q000166:cytology* / Q000187:drug effects; D001696:Biomechanical Phenomena / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D005260:Female; D006350:Heart Valve Prosthesis; D007150:Immunohistochemistry; D012038:Regeneration / Q000187:drug effects*; D012756:Sheep; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*; D014694:Ventricular Outflow Obstruction / Q000628:therapy
chemical_list: D012967:Sodium Dodecyl Sulfate; D002118:Calcium