title: in vitro and in silico determination of oral, jejunum and Caco-2 human absorption of fatty acids and polyphenols. Micellar liquid chromatography.
abstract: In this investigation chosen saturated, mono- and polyunsaturated fatty acids as well as polyphenols have been analyzed. The main aim of this study was to determine oral, jejunum and Caco-2 human absorption of chosen fatty acids and polyphenols using in vitro and in silico methods. For in vitro determination of human drug absorption, the usefulness of Micellar Liquid Chromatography (MLC) with mobile phases containing different surfactants (including Brij35-Biopartitioning Micellar Chromatography (BMC)) has been confirmed. On the basis of Foley's equation, 1/k vs. CM correlations for the tested compounds have been done. Satisfactory linearity of the relationships was found over the whole eluents composition range studied with R(2) approximately 0.99 in each case. Moreover, the analyte-micelle association constants (Kma) from Foley's equation have been compared for different micellar environments, containing Brij35, SDS and CTAB as a main component of micellar mobile phases. Completely new models describing human oral as well as Caco-2 and jejunum absorption have been constructed and compared with the cited models. These models are based on the Abraham descriptors and lipophilicity parameters as well as steric descriptors. Furthermore, many different correlations between physicochemical parameters and human intestinal absorption have been done, e.g. the correlation between human jejunum permeability estimated in silico and received using LSER parameters was excellent (R(2) nearly 0.99). Chromatographic parameters have been collated with steric, electronic and physicochemical ones using QRAR (Quantitative Retention - Activity Relationships) and QSAR (Quantitative Structure - Activity Relationships) models. Moreover, retention BMC data have been compared with lipophilicity parameter logPo/w (n-octanol-water partition coefficient). The influence of lipophilicity on oral absorption (%) has been checked. The correlation between predicted oral absorption (%) and logPo/w has been done. Obtained R(2) was 0.82. On the basis of chromatographic, lipophilicity, steric and different physicochemical parameters, the principal components analysis (PCA) has been done.
authors.affiliation: Faculty of Chemistry, Chair of Physical Chemistry, Department of Planar Chromatography, Maria Curie - Skłodowska University, M. Curie - Skłodowska Sq. 3, 20-031 Lublin, Poland. Electronic address: katarzyna.stepnik@poczta.umcs.lublin.pl.
authors.forename: Katarzyna E
authors.initials: KE
authors.lastname: Stępnik
authors.affiliation: Faculty of Chemistry, Chair of Physical Chemistry, Department of Planar Chromatography, Maria Curie - Skłodowska University, M. Curie - Skłodowska Sq. 3, 20-031 Lublin, Poland.
authors.forename: Irena
authors.initials: I
authors.lastname: Malinowska
authors.affiliation: Fertilizer Research Institute, Tysiąclecia Państwa Polskiego Ave.13a, 24-110 Puławy, Poland.
authors.forename: Edward
authors.initials: E
authors.lastname: Rój
mesh_terms: D018938:Caco-2 Cells; D002853:Chromatography, Liquid / Q000379:methods*; D003198:Computer Simulation; D005227:Fatty Acids / Q000032:analysis*; D006801:Humans; D066298:In Vitro Techniques; D007408:Intestinal Absorption; D007583:Jejunum / Q000737:chemistry* / Q000378:metabolism; D008823:Micelles; D008954:Models, Biological; D009061:Mouth Mucosa / Q000737:chemistry* / Q000378:metabolism; D059808:Polyphenols / Q000032:analysis*; D025341:Principal Component Analysis
chemical_list: D005227:Fatty Acids; D008823:Micelles; D059808:Polyphenols
keywords: Biopartitioning Micellar Chromatography; Caco-2 cell lines; Jejunum absorption; Micellar Liquid Chromatography; Oral drug absorption