title: [Effects of glucose variation on hexose monophosphate shunt and leucocyte respiratory burst in patients with type 2 diabetes].
abstract: OBJECTIVE
  To investigate the effect of blood glucose instability on respiratory burst of leukocytes in patients with type 2 diabetes (T2DM).
  
  
  METHODS
  Forty-five patients with T2DM were divided into 3 groups after continuous glucose monitoring for 72 h with glucose wavy coefficient <1.5 (n=11), between 1.5 and 3.0 (n=19), and >3.0 (n=15). Peripheral blood neutrophils were isolated from the diabetic patients and normal control subjects for assay of glucose 6-phosphate dehydrogenase (G6PD) with a spectrophotometric method, detecting G6PD mRNA expression by real-time PCR, and determining reactive oxygen species level using the fluorescent probe DCFH-DA.
  
  
  RESULTS
  Compared with the normal control group, the diabetic patients showed significantly lowered G6PD activity (F=78.739, P<0.05) and ROS level (F=384.962, P<0.05) but significantly increased G6PD mRNA expression (F=269.612, P<0.01). These changes were significantly correlated with the blood glucose wavy coefficients.
  
  
  CONCLUSIONS
  The fluctuation of blood glucose in T2DM patients can decrease G6PD activity and lead to functional decline of the respiratory burst.
authors.affiliation: Department of Endocrinology, Guangdong Provincical Hospital of Traditional Chinese Medicine, Guangzhou 510120, China.E-mail: 13924011881@126.com.
authors.forename: Huiyan
authors.initials: H
authors.lastname: Zeng
authors.forename: Ying
authors.initials: Y
authors.lastname: Cao
authors.forename: Yaoming
authors.initials: Y
authors.lastname: Xue
mesh_terms: D001786:Blood Glucose / Q000737:chemistry*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006801:Humans; D009504:Neutrophils / Q000378:metabolism; D010427:Pentose Phosphate Pathway; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst
chemical_list: D001786:Blood Glucose; D017382:Reactive Oxygen Species; D005954:Glucosephosphate Dehydrogenase