title: Ca2+-controlled assembly for visualized detection of conformation changes of calmodulin.
abstract: A new strategy has been designed for visualized detection of the conformation changes of calmodulin bound to target peptide (CaM-M13) based on the conformation sensitive property of a water-soluble conjugated polythiophene derivative (PMNT) and the electrostatic interactions of PMNT/CaM-M13. Interestingly, the direct visualized PMNT color changes under UV irradiation and the turbidity changes of samples in aqueous medium can be applied to detect the conformation changes as well as the controllable assembly of PMNT/CaM-M13 with Ca(2+) in aqueous medium. Because of the specific binding of Ca(2+), the assembly of PMNT/CaM-M13 can be applied to sense calcium as well.
authors.affiliation: Key Laboratory of Hebei Province for Molecular Biophysics, Institute of Biophysics, Hebei University of Technology , Tianjin 300401, P. R. China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Yuan
authors.forename: Chengfen
authors.initials: C
authors.lastname: Xing
authors.forename: Hailong
authors.initials: H
authors.lastname: An
authors.forename: Ruimin
authors.initials: R
authors.lastname: Niu
authors.forename: Ruihua
authors.initials: R
authors.lastname: Li
authors.forename: Wenmin
authors.initials: W
authors.lastname: Yan
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhan
mesh_terms: D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000737:chemistry*; D008027:Light; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D055672:Static Electricity; D013876:Thiophenes / Q000737:chemistry
chemical_list: D002147:Calmodulin; D010455:Peptides; D011108:Polymers; D013876:Thiophenes; C066730:polythiophene; D002118:Calcium
keywords: CaM-M13; assembly; calcium; conformation changes; conjugated polythiophene