title: Could FaRP-Like Peptides Participate in Regulation of Hyperosmotic Stress Responses in Plants?
abstract: The ability to respond to hyperosmotic stress is one of the numerous conserved cellular processes that most of the organisms have to face during their life. In metazoans, some peptides belonging to the FMRFamide-like peptide (FLP) family were shown to participate in osmoregulation via regulation of ion channels; this is, a well-known response to hyperosmotic stress in plants. Thus, we explored whether FLPs exist and regulate osmotic stress in plants. First, we demonstrated the response of Arabidopsis thaliana cultured cells to a metazoan FLP (FLRF). We found that A. thaliana express genes that display typical FLP repeated sequences, which end in RF and are surrounded by K or R, which is typical of cleavage sites and suggests bioactivity; however, the terminal G, allowing an amidation process in metazoan, seems to be replaced by W. Using synthetic peptides, we showed that amidation appears unnecessary to bioactivity in A. thaliana, and we provide evidence that these putative FLPs could be involved in physiological processes related to hyperosmotic stress responses in plants, urging further studies on this topic.
authors.affiliation: Sorbonne Paris Cité, Institut des Energies de Demain, Université Paris Diderot , Paris , France ; LINV-DiSPAA, Department of Agri-Food and Environmental Science, University of Florence , Sesto Fiorentino , Italy.
authors.forename: François
authors.initials: F
authors.lastname: Bouteau
authors.affiliation: Muséum National d'Histoire Naturelle, DMPA, Sorbonne Universités, UMR BOREA MNHN-CNRS 7208-IRD 207-UPMC-UCBN , Paris , France ; Faculté des Sciences and Technologies, Université Paris Est Créteil-Val de Marne (UPEC) , Créteil , France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Bassaglia
authors.affiliation: Sorbonne Paris Cité, Institut des Energies de Demain, Université Paris Diderot , Paris , France ; LINV-DiSPAA, Department of Agri-Food and Environmental Science, University of Florence , Sesto Fiorentino , Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Monetti
authors.affiliation: Sorbonne Paris Cité, Institut des Energies de Demain, Université Paris Diderot , Paris , France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Tran
authors.affiliation: Sorbonne Paris Cité, Institut des Energies de Demain, Université Paris Diderot , Paris , France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Navet
authors.affiliation: LINV-DiSPAA, Department of Agri-Food and Environmental Science, University of Florence , Sesto Fiorentino , Italy ; Sorbonne Paris Cité, Paris Interdisciplinary Energy Research Institute (PIERI), Université Paris Diderot , Paris , France.
authors.forename: Stefano
authors.initials: S
authors.lastname: Mancuso
authors.affiliation: UPMC UMR7622-IBPS , Paris , France.
authors.forename: Hayat
authors.initials: H
authors.lastname: El-Maarouf-Bouteau
authors.affiliation: Muséum National d'Histoire Naturelle, DMPA, Sorbonne Universités, UMR BOREA MNHN-CNRS 7208-IRD 207-UPMC-UCBN , Paris , France.
authors.forename: Laure
authors.initials: L
authors.lastname: Bonnaud-Ponticelli
keywords: Arabidopsis thaliana; FaRP-like peptides; drought; osmotic stress; stomata