title: Chemical biology of genomic DNA: minimizing PCR bias.
abstract: The exquisite selectivity of chemical reactions enables the study of rare DNA bases. However, chemical modification of the genome can affect downstream analysis. We report a PCR bias caused by such modification, and exemplify a solution with the synthesis and characterization of a cleavable aldehyde-reactive biotinylation probe.
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK. sb10031@cam.ac.uk.
authors.forename: Gordon R
authors.initials: GR
authors.lastname: McInroy
authors.forename: Eun-Ang
authors.initials: EA
authors.lastname: Raiber
authors.forename: Shankar
authors.initials: S
authors.lastname: Balasubramanian
mesh_terms: D000447:Aldehydes / Q000737:chemistry; D019921:Biotinylation; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D018736:DNA Adducts / Q000737:chemistry; D015342:DNA Probes / Q000138:chemical synthesis / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000447:Aldehydes; D018736:DNA Adducts; D015342:DNA Probes; D004247:DNA