title: Improved method for chemiluminescent determination of peroxidase-mimicking DNAzyme activity.
abstract: The optimization of experimental conditions for the chemiluminescent determination of peroxidase-mimicking DNAzyme (PMDNAzyme) formed at the interaction of hemin and its aptamer EAD2 was performed. The effect of concentrations of hydrogen peroxide and luminol, acidity of the substrate solution, and composition and concentration of the assay buffer was estimated. Under optimized conditions, a value of detection limit for the PMDNAzyme was 350 pM. A comparison of the conditions determined in this work with those reported previously showed that the optimization of the composition of the substrate solution improved the sensitivity of the chemiluminescent determination of the PMDNAzyme. The obtained results open up promising perspectives for using the proposed method to improve the sensitivity of PMDNAzyme-based assays.
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia.
authors.forename: Anastasia V
authors.initials: AV
authors.lastname: Gribas
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, College of Chemistry and Pharmacy, Guangxi Normal University, Guilin 541004, China.
authors.forename: Shulin
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia. Electronic address: sakharovivan@gmail.com.
authors.forename: Ivan Yu
authors.initials: IY
authors.lastname: Sakharov
mesh_terms: D001681:Biological Assay / Q000379:methods*; D021881:DNA, Catalytic / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods* / Q000592:standards; D057230:Limit of Detection; D008163:Luminescent Measurements; D009195:Peroxidase / Q000378:metabolism*
chemical_list: D021881:DNA, Catalytic; D009195:Peroxidase
keywords: Aptamer; Chemiluminescence; DNAzyme; Hemin; Mimetics; Peroxidase