title: Semen quality of stallions challenged with the Kentucky 84 strain of equine arteritis virus.
abstract: Equine arteritis virus (EAV) is the causal agent of equine viral arteritis (EVA), a respiratory and reproductive disease of equids. Some strains of EAV can cause fever, leukopenia, and dependent edema of the limbs, scrotum, and preputium in the acutely infected stallion. We hypothesized that fever and scrotal edema observed during the acute phase of the infection, but not the presence of EAV, have an adverse effect on semen quality. A group of seven stallions were intranasally inoculated with the Kentucky 84 (KY84) strain of EAV. Stallions were monitored for clinical signs of EVA until 42 days postinoculation (dpi). Semen was collected every other day for the first 15 days and 2 times a week up to 79 dpi. Additional samples were collected at 147, 149, and 151 dpi. Semen from each stallion was evaluated on the basis of motion characteristics, total number of spermatozoa, membrane integrity, and morphology. Virus infectivity titers were determined in RK-13 cells. Significant decreases in sperm quality were observed between 9 and 76 dpi. LOWESS (locally weighted scatterplot smoothing) curves for each horse were fit and integrated to quantify spermatozoa exposure to fever, virus, and edema over a period of 67 days before each ejaculation. Linear mixed models were then fit to isolate the effects of each factor on semen quality. Scrotal edema and fever were found to exert independent effects on all the semen quality parameters (P ≤ 0.002), whereas virus seems to exert little to no direct effect, as virus titers remained high long after semen quality returned to baseline.
authors.affiliation: Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, Lexington, Kentucky, USA.
authors.forename: Juliana R
authors.initials: JR
authors.lastname: Campos
authors.affiliation: Department of Biostatistics, University of Kentucky, Lexington, Kentucky, USA.
authors.forename: Patrick
authors.initials: P
authors.lastname: Breheny
authors.affiliation: University of Sao Paulo, Laboratory of Molecular Morphophysiology and Development, FZEA-USP, Pirassununga, Sao Paulo, Brazil.
authors.forename: Reno R
authors.initials: RR
authors.lastname: Araujo
authors.affiliation: Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, Lexington, Kentucky, USA.
authors.forename: Mats H T
authors.initials: MH
authors.lastname: Troedsson
authors.affiliation: Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, Lexington, Kentucky, USA.
authors.forename: Edward L
authors.initials: EL
authors.lastname: Squires
authors.affiliation: Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, Lexington, Kentucky, USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Timoney
authors.affiliation: Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, Lexington, Kentucky, USA. Electronic address: ubalasuriya@uky.edu.
authors.forename: Udeni B R
authors.initials: UB
authors.lastname: Balasuriya
mesh_terms: D000818:Animals; D018174:Arterivirus Infections / Q000503:physiopathology / Q000662:veterinary*; D002462:Cell Membrane / Q000648:ultrastructure; D004487:Edema; D007240:Equartevirus; D005334:Fever; D006734:Horse Diseases / Q000503:physiopathology* / Q000821:virology*; D006736:Horses; D007629:Kentucky; D008297:Male; D012611:Scrotum; D055101:Semen Analysis / Q000662:veterinary*; D013076:Sperm Count / Q000662:veterinary; D013081:Sperm Motility; D013094:Spermatozoa / Q000002:abnormalities / Q000648:ultrastructure*
keywords: EAV; EVA; Equine arteritis virus; Equine viral arteritis; Semen evaluation; Semen quality