title: Hemagglutinin 222D/G polymorphism facilitates fast intra-host evolution of pandemic (H1N1) 2009 influenza A viruses.
abstract: The amino acid substitution of aspartic acid to glycine in hemagglutinin (HA) in position 222 (HA-D222G) as well as HA-222D/G polymorphism of pandemic (H1N1) 2009 influenza viruses (A(H1N1)pdm09) were frequently reported in severe influenza in humans and mice. Their impact on viral pathogenicity and the course of influenza has been discussed controversially and the underlying mechanism remained unclarified. In the present study, BALB/c mice, infected with the once mouse lung- and cell-passaged A(H1N1)pdm09 isolate A/Jena/5258/09 (mpJena/5258), developed severe pneumonia. From day 2 to 3 or 4 post infection (p.i.) symptoms (body weight loss and clinical score) continuously worsened. After a short disease stagnation or even recovery phase in most mice, severity of disease further increased on days 6 and 7 p.i. Thereafter, surviving mice recovered. A 45 times higher virus titer maximum in the lung than in the trachea on day 2 p.i. and significantly higher tracheal virus titers compared to lung on day 6 p.i. indicated changes in the organ tropism during infection. Sequence analysis revealed an HA-222D/G polymorphism. HA-D222 and HA-G222 variants co-circulated in lung and trachea. Whereas, HA-D222 variant predominated in the lung, HA-G222 became the major variant in the trachea after day 4 p.i. This was accompanied by lower neutralizing antibody titers and broader receptor recognition including terminal sialic acid α-2,3-linked galactose, which is abundant on mouse trachea epithelial cells. Plaque-purified HA-G222-mpJena/5258 virus induced severe influenza with maximum symptom on day 6 p.i. These results demonstrated for the first time that HA-222D/G quasispecies of A(H1N1)pdm09 caused severe biphasic influenza because of fast viral intra-host evolution, which enabled partial antibody escape and minor changes in receptor binding.
authors.affiliation: Jena University Hospital, Friedrich Schiller University Jena, Department of Virology and Antiviral Therapy, Jena, Germany.
authors.forename: Nora
authors.initials: N
authors.lastname: Seidel
authors.affiliation: Jena University Hospital, Friedrich Schiller University Jena, Department of Virology and Antiviral Therapy, Jena, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Sauerbrei
authors.affiliation: Jena University Hospital, Friedrich Schiller University Jena, Department of Virology and Antiviral Therapy, Jena, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Wutzler
authors.affiliation: Jena University Hospital, Friedrich Schiller University Jena, Department of Virology and Antiviral Therapy, Jena, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Schmidtke
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood*; D001483:Base Sequence; D019143:Evolution, Molecular; D015870:Gene Expression; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics* / Q000276:immunology; D058507:Host Specificity; D006801:Humans; D057131:Immune Evasion; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics* / Q000276:immunology; D008168:Lung / Q000276:immunology* / Q000473:pathology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000473:pathology / Q000821:virology; D011110:Polymorphism, Genetic; D011991:Receptors, Virus / Q000737:chemistry / Q000276:immunology; D012794:Sialic Acids / Q000737:chemistry / Q000276:immunology; D014132:Trachea / Q000276:immunology* / Q000473:pathology / Q000821:virology; D056189:Viral Tropism
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011991:Receptors, Virus; D012794:Sialic Acids; C483468:hemagglutinin, human influenza A virus