title: Immunocytochemical study of YB-1 nuclear distribution in different cell types.
abstract: In the present work we studied the distribution of YB-1 in the nuclei of mouse hepatocytes, early embryos and human skin fibroblasts with the use of light and electron microscopy. To reveal YB-1, we applied rat polyclonal antibody against the C-terminal fragment of YB-1 molecule and rabbit polyclonal antibody against full-length YB-1 molecule. YB-1 distribution patterns varied significantly in different cell types. YB-1 was found to be colocalized with RNA polymerase I in mouse hepatocytes and embryos. Besides, YB-1 was revealed in a population of Cajal bodies in 2-cell mouse embryos but not in other cells studied.
authors.affiliation: Laboratory of Cell Morphology, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Avenue, 194064 St. Petersburg, Russia. Electronic address: ibogol@mail.ru.
authors.forename: I O
authors.initials: IO
authors.lastname: Bogolyubova
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, 4 Institutskaya Street, 142290 Pushchino, Moscow Region, Russia.
authors.forename: D N
authors.initials: DN
authors.lastname: Lyabin
authors.affiliation: Laboratory of Cell Morphology, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Avenue, 194064 St. Petersburg, Russia.
authors.forename: D S
authors.initials: DS
authors.lastname: Bogolyubov
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, 4 Institutskaya Street, 142290 Pushchino, Moscow Region, Russia.
authors.forename: L P
authors.initials: LP
authors.lastname: Ovchinnikov
mesh_terms: D000818:Animals; D019070:Cell Lineage; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D020541:Coiled Bodies / Q000648:ultrastructure; D004622:Embryo, Mammalian / Q000378:metabolism / Q000648:ultrastructure*; D005347:Fibroblasts / Q000648:ultrastructure; D022781:Hepatocytes / Q000648:ultrastructure*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008854:Microscopy, Electron; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D014157:Transcription Factors; C541744:Ybx1 protein, mouse
keywords: Cajal bodies; Cell nucleus; RNA polymerase I; YB-1