title: Preparation and characterization of monoclonal antibody against human B7-H4 molecule.
abstract: B7-H4, a member of B7 family, is widely expressed in tumor tissues and plays an important role in the negative regulation of T cell immunity. In this study, we report on the establishment and characterization of a functional anti-human B7-H4 monoclonal antibody (MAb) 5G3 through hybridoma method. Flow cytometry analysis showed that MAb 5G3 specifically bound to B7-H4 molecule. Functional experiments indicated that MAb 5G3 could block the inhibitory role of B7-H4 molecule on A549 cells in and reduce the apoptosis of Jurkat cells, suggesting that MAb 5G3 is an antagonistic antibody and a useful tool for further studies of B7-H4 functions in cancers.
authors.affiliation: 1 Department of Immunology, School of Medicine, Soochow University , Suzhou, China .
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Fang
authors.forename: Zong-Jiang
authors.initials: ZJ
authors.lastname: Gu
mesh_terms: D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology*; D002460:Cell Line; D000939:Epitopes / Q000235:genetics; D005434:Flow Cytometry; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D019169:Jurkat Cells; D060907:V-Set Domain-Containing T-Cell Activation Inhibitor 1 / Q000276:immunology* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D060907:V-Set Domain-Containing T-Cell Activation Inhibitor 1; C475826:VTCN1 protein, human