title: First evaluation of a multi-capillary electrophoresis CDT assay on Helena Biosciences' V8 analyser.
abstract: BACKGROUND
  Carbohydrate-deficient transferrin measurement is currently used for the routine monitoring of excessive alcohol intake, thus playing a fundamental role in the management of alcohol consumption disorders as well as for medico-legal purposes. The aim of the present paper is to report the results obtained from the first performance evaluation of a new CDT quantification assay, and to assess its suitability in routine work.
  
  
  METHODS
  We assessed the analytical performances of the multi-capillary electrophoresis analyser Helena Biosciences' V8 E-class and compared the results obtained with those from the HPLC BioRad Ready-Prep CDT assay. Furthermore, we evaluated the robustness of the system in a routine work conditions.
  
  
  RESULTS
  Within laboratory imprecision CV% (n=40) using four commercially available quality control materials and two serum pool samples with different concentrations of CDT were <11.2%. The comparison made with the established method was CDT [V8capillary electrophoresis]=0.84 × CDT [HPLC]+0.03, with a Pearson coefficient of r=0.970, and with the Bland-Altman plot showing a significant bias -0.3 (-0.4 to -0.22, 95% CI). The obtained accuracy was highly satisfactory.
  
  
  CONCLUSIONS
  The findings made in the present study indicated that the proposed analytical system is a valid alternative to other CDT screening assays currently proposed for routine use in clinical laboratories, since it is precise, accurate and robust, with a high throughput. Moreover, investment in the training of staff is of fundamental importance in ensuring correct interpretation of the electrophoretic pattern, thus providing reliable CDT results.
authors.affiliation: Department of Laboratory Medicine, University-Hospital, Padova, Italy.
authors.forename: Mariela
authors.initials: M
authors.lastname: Marinova
authors.affiliation: Department of Laboratory Medicine, University-Hospital, Padova, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Artusi
authors.affiliation: Department of Laboratory Medicine, University-Hospital, Padova, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Baggio
authors.affiliation: Department of Laboratory Medicine, University-Hospital, Padova, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Zaninotto
authors.affiliation: Department of Laboratory Medicine, University-Hospital, Padova, Italy; Department of Medicine, University of Padova, Italy. Electronic address: mario.plebani@unipd.it.
authors.forename: Mario
authors.initials: M
authors.lastname: Plebani
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D014168:Transferrin / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D014168:Transferrin; C056759:carbohydrate-deficient transferrin
keywords: CDT; Carbohydrate-deficient transferrin; HPLC; Multi-capillary electrophoresis