title: Scopoletin from Cirsium setidens Increases Melanin Synthesis via CREB Phosphorylation in B16F10 Cells.
abstract: In this study, we isolated scopoletin from Cirsium setidens Nakai (Compositae) and tested its effects on melanogenesis. Scopoletin was not toxic to cells at concentrations less than 50 µM and increased melanin synthesis in a dose-dependent manner. As melanin synthesis increased, scopoletin stimulated the total tyrosinase activity, the rate-limiting enzyme of melanogenesis. In a cell-free system, however, scopoletin did not increase tyrosinase activity, indicating that scopoletin is not a direct activator of tyrosinase. Furthermore, Western blot analysis showed that scopoletin stimulated the production of microphthalmia-associated transcription factor (MITF) and tyrosinase expression via cAMP response element-binding protein (CREB) phosphorylation in a dose-dependent manner. Based on these results, preclinical and clinical studies are needed to assess the use of scopoletin for the treatment of vitiligo.
authors.affiliation: Department of Pharmacognosy, Chung-Ang University College of Pharmacy, Seoul 156-756, Korea.
authors.forename: Mi-Ja
authors.initials: MJ
authors.lastname: Ahn
authors.affiliation: Department of Pharmacognosy, Chung-Ang University College of Pharmacy, Seoul 156-756, Korea.
authors.forename: Sun-Jung
authors.initials: SJ
authors.lastname: Hur
authors.affiliation: Department of Biochemistry, Chung-Ang University College of Medicine, Seoul 156-756, Korea.
authors.forename: Eun-Hyun
authors.initials: EH
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, Chung-Ang University College of Medicine, Seoul 156-756, Korea.
authors.forename: Seung Hoon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Biochemistry, Chung-Ang University College of Medicine, Seoul 156-756, Korea.
authors.forename: Jun Seob
authors.initials: JS
authors.lastname: Shin
authors.affiliation: Thomas J. Long School of Pharmacy, University of the Pacific, CA 95211, USA.
authors.forename: Myo-Kyoung
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Thomas J. Long School of Pharmacy, University of the Pacific, CA 95211, USA.
authors.forename: James A
authors.initials: JA
authors.lastname: Uchizono
authors.affiliation: Department of Pharmacognosy, Chung-Ang University College of Pharmacy, Seoul 156-756, Korea.
authors.forename: Wan-Kyunn
authors.initials: WK
authors.lastname: Whang
authors.affiliation: Department of Biochemistry, Chung-Ang University College of Medicine, Seoul 156-756, Korea.
authors.forename: Dong-Seok
authors.initials: DS
authors.lastname: Kim
keywords: CREB; Cirsium setidens; MITF; Scopoletin; Tyrosinase