title: Generation and characterization of polyclonal antibody against part of immunoglobulin constant heavy υ chain of goose.
abstract: Immunoglobulin Y (abbreviated as IgY) is a type of immunoglobulin that is the major antibody in bird, reptile, and lungfish blood. IgY consists of two light (λ) and two heavy (υ) chains. In the present study, polyclonal antibody against IgYFc was generated and evaluated. rIgYCυ3/Cυ4 was expressed in Escherichia coli, purified and utilized to raise polyclonal antibody in rabbit. High affinity antisera were obtained, which successfully detected the antigen at a dilution of 1:204,800 for ELISA assay. The antibody can specifically recognize both rIgYCυ3/Cυ4 and native IgY by Western bolt analysis. Furthermore, the serum of Grus japonensis or immunoglobulin of chicken, duck, turkey, and silkie samples and dynamic changes of serum GoIgY after immunogenicity with GPV-VP3-virus-like particles (GPV-VP3-VLPs) can be detected with the anti-GoIgYFc polyclonal antibody. These results suggested that the antibody is valuable for the investigation of biochemical properties and biological functions of GoIgY.
authors.affiliation: 1 College of Wildlife Resources, Northeast Forestry University , Harbin, China .
authors.forename: Panpan
authors.initials: P
authors.lastname: Zhao
authors.forename: Yongli
authors.initials: Y
authors.lastname: Guo
authors.forename: Bo
authors.initials: B
authors.lastname: Ma
authors.forename: Mingwei
authors.initials: M
authors.lastname: Xing
authors.forename: Junwei
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D005777:Geese / Q000276:immunology*; D007127:Immunoglobulin Constant Regions / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000276:immunology*; D007136:Immunoglobulins / Q000276:immunology*; D010957:Plasmids / Q000235:genetics
chemical_list: D000906:Antibodies; C016512:IgY; D007127:Immunoglobulin Constant Regions; D007143:Immunoglobulin Heavy Chains; D007136:Immunoglobulins