title: Devolopmental and growth temperature regulation of omega-3 fatty acid desaturase genes in safflower (Carthamus tinctorius L.).
abstract: Three ω-3 fatty acid desaturase genes (CtFAD3, CtFAD7, and CtFAD8) were isolated from safflower (Carthamus tinctorius L.). Transcript analysis showed that the highest transcript levels were detected for CtFAD3 and the low transcript levels were detected for CtFAD7 and CtFAD8 in flowers. This result indicates that CtFAD3 enzyme activity is important for fatty acid desaturation in flowers. The low transcript level of CtFAD3 in developing seeds was consistent with the recorded high level of linoleic acid (18:2) and lack of linolenic acid (18:3) in safflower seed oil. At low temperatures, the induced transcription levels of ω-3 fatty acid desaturase genes in the stems and petioles were consistent with increased polyunsaturated fatty acids (PUFAs). In the roots, ω-3 fatty acid desaturase noticeably increased at low temperatures, whereas PUFA levels decreased. Interestingly, C18:3(Δ9,12,15) alcohol was specifically found in safflower roots, and showed a significant increase, indicating a flux in the acid to alcohol ratio of this compound in safflower roots.
authors.affiliation: Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, China.
authors.forename: L-L
authors.initials: LL
authors.lastname: Guan
authors.affiliation: Agronomy College, Sichuan Agricutural University, Cheng Du, China ewuwei@gmail.com.
authors.forename: W
authors.initials: W
authors.lastname: Wu
authors.affiliation: Agronomy College, Sichuan Agricutural University, Cheng Du, China.
authors.forename: B
authors.initials: B
authors.lastname: Hu
authors.affiliation: Agronomy College, Sichuan Agricutural University, Cheng Du, China.
authors.forename: D
authors.initials: D
authors.lastname: Li
authors.affiliation: Agronomy College, Sichuan Agricutural University, Cheng Du, China.
authors.forename: J-W
authors.initials: JW
authors.lastname: Chen
authors.affiliation: Agronomy College, Sichuan Agricutural University, Cheng Du, China.
authors.forename: K
authors.initials: K
authors.lastname: Hou
authors.affiliation: Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, China.
authors.forename: L
authors.initials: L
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D031187:Carthamus tinctorius / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D044943:Fatty Acid Desaturases / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D035264:Flowers / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D007527:Isoenzymes / Q000145:classification / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D018547:Plant Stems / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013696:Temperature
chemical_list: D018076:DNA, Complementary; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D007527:Isoenzymes; D010940:Plant Proteins; D044943:Fatty Acid Desaturases; C078565:omega-3 fatty acid desaturase