title: ChIP for Hox proteins from Drosophila imaginal discs.
abstract: Chromatin immunoprecipitation (ChIP) is a technique that reveals in vivo location of a protein bound to DNA. ChIP coupled with DNA microarrays (ChIP-chip) or next-generation sequencing (ChIP-seq) allows for identification of binding sites of transcription factors on a global scale. Here we describe a protocol for ChIP to identify binding of the Ultrabithorax (Ubx) Hox transcription factors from imaginal discs of Drosophila larvae. The protocol can be extended to other model organisms and transcription factors.
authors.affiliation: Janelia Farm Research Campus, HHMI, Ashburn, VA, 20147, USA, pavan.agrawal123@gmail.com.
authors.forename: Pavan
authors.initials: P
authors.lastname: Agrawal
authors.forename: L S
authors.initials: LS
authors.lastname: Shashidhara
mesh_terms: D000818:Animals; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D047369:Chromatin Immunoprecipitation / Q000379:methods; D004330:Drosophila / Q000378:metabolism*; D059014:High-Throughput Nucleotide Sequencing; D018398:Homeodomain Proteins / Q000378:metabolism*; D060227:Imaginal Discs / Q000378:metabolism*
chemical_list: D002843:Chromatin; D018398:Homeodomain Proteins