title: Expressed proteins of Herbaspirillum seropedicae in maize (DKB240) roots-bacteria interaction revealed using proteomics.
abstract: Several molecular tools have been used to clarify the basis of plant-bacteria interaction; however, the mechanism behind the association is still unclear. In this study, we used a proteomic approach to investigate the root proteome of Zea mays (cv. DKB240) inoculated with Herbaspirillum seropedicae strain SmR1 grown in vitro and harvested 7 days after inoculation. Eighteen differentially accumulated proteins were observed in root samples, ten of which were identified by MALDI-TOF mass spectrometry peptide mass fingerprint. Among the identified proteins, we observed three proteins present exclusively in inoculated root samples and six upregulated proteins and one downregulated protein relative to control. Differentially expressed maize proteins were identified as hypothetical protein ZEAMMB73_483204, hypothetical protein ZEAMMB73_269466, and tubulin beta-7 chain. The following were identified as H. seropedicae proteins: peroxiredoxin protein, EF-Tu elongation factor protein, cation transport ATPase, NADPH:quinone oxidoreductase, dinitrogenase reductase, and type III secretion ATP synthase. Our results presented the first evidence of type III secretion ATP synthase expression during H. seropedicae-maize root interaction.
authors.affiliation: Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Rod. Admar Gonzaga, 1346, 88034-001, Florianópolis, SC, Brazil.
authors.forename: Cibele Santos
authors.initials: CS
authors.lastname: Ferrari
authors.forename: Fernanda Plucani
authors.initials: FP
authors.lastname: Amaral
authors.forename: Jessica Cavalheiro Ferreira
authors.initials: JC
authors.lastname: Bueno
authors.forename: Mirella Christine
authors.initials: MC
authors.lastname: Scariot
authors.forename: Pedro Alexandre
authors.initials: PA
authors.lastname: Valentim-Neto
authors.forename: Ana Carolina Maisonnave
authors.initials: AC
authors.lastname: Arisi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D060026:Endophytes / Q000378:metabolism / Q000502:physiology; D015870:Gene Expression; D042803:Herbaspirillum / Q000378:metabolism / Q000502:physiology*; D018517:Plant Roots / Q000235:genetics* / Q000382:microbiology*; D040901:Proteomics; D003313:Zea mays / Q000235:genetics* / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins