title: Ethanol at levels produced by Saccharomyces cerevisiae during wheat dough fermentation has a strong impact on dough properties.
abstract: Yeast's role in bread making is primarily the fermentative production of carbon dioxide to leaven the dough. Fermentation also impacts dough matrix rheology, thereby affecting the quality of the end product. Surprisingly, the role of ethanol, the other yeast primary metabolite, has been ill studied in this context. Therefore, this study aims to assess the potential impact of ethanol on yeastless dough extensibility and spread and gluten agglomeration at concentrations at which it is produced in fermenting dough, i.e., up to 60 mmol per 100 g of flour. Reduced dough extensibility and dough spread were observed upon incorporation of ethanol in the dough formula, and were more pronounced for a weak than for a strong flour. Uniaxial and biaxial extension tests showed up to 50% decrease in dough extensibility and a dough strength increase of up to 18% for 60 mmol of ethanol/100 g of flour. Ethanol enhanced gluten agglomeration of a weak flour. Sequential extraction of flour in increasing ethanol concentrations showed that better gluten-solvent interaction is a possible explanation for the changed dough behavior.
authors.affiliation: Laboratory of Food Chemistry and Biochemistry & Leuven Food Science and Nutrition Research Centre (LFoRCe), KU Leuven , Kasteelpark Arenberg 20, Box 2463, B-3001 Leuven, Belgium.
authors.forename: Vinay B
authors.initials: VB
authors.lastname: Jayaram
authors.forename: Mohammad N
authors.initials: MN
authors.lastname: Rezaei
authors.forename: Sven
authors.initials: S
authors.lastname: Cuyvers
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Verstrepen
authors.forename: Jan A
authors.initials: JA
authors.lastname: Delcour
authors.forename: Christophe M
authors.initials: CM
authors.lastname: Courtin
mesh_terms: D001939:Bread / Q000032:analysis* / Q000382:microbiology; D000431:Ethanol / Q000032:analysis / Q000378:metabolism*; D005285:Fermentation; D005433:Flour / Q000032:analysis / Q000382:microbiology; D005983:Glutens / Q000032:analysis / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014908:Triticum / Q000378:metabolism / Q000382:microbiology*
chemical_list: D000431:Ethanol; D005983:Glutens
keywords: Alveograph; Kieffer rig dough extensibility; ethanol; fermenting dough; gluten starch separation; yeast