title: Evaluation of the adjuvant activity of propranolol, a Beta-adrenergic receptor antagonist, on efficacy of a malaria vaccine model in BALB/c mice.
abstract: We have previously shown the adjuvant activity of propranolol (PRP) (a beta-adrenergic receptor antagonist) using a vaccine model for Salmonella typhimurium. In this study PRP was used as an adjuvant in combination with Plasmodium berghei (P. berghei) whole blood stage (PWBS) antigens. BALB/c mice were immunized three times with a 2-week interval, either PWBS vaccine alone or in combination with the adjuvant alum or propranolol. The control group received phosphate buffered saline. Evaluation of the cellular and humoral immunity was performed by measurement of interferon (IFN)-γ, tumor necrosis factor (TNF)-α, lymphocyte proliferation, total IgG and IgG2a in the control and immunized groups. Furthermore, Clinical evaluations were carried out by analyze survival rate and parasitemia of the mice. Our results showed that the mice immunized with propranolol induced higher levels of antibody, IFN-γ and TNF-α as well as stronger lymphocyte proliferative responses compared with other groups. This resulted in improved protective immunity against Plasmodium berghei. Administration of the PRP as an adjuvant in combination with the PWBS Antigen vaccine can shift the immune responses to a T helper1 pattern and enhance the protective immunity.
authors.affiliation: Cellular and Molecular Research Center; Urmia University of Medical Sciences, Urmia, Iran and Department of Microbiology, Immunology and Genetics, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran. shahabirabori@yahoo.com.
authors.forename: Shahram
authors.initials: S
authors.lastname: Shahabi
authors.affiliation: Department of Medical Parasitology and Mycology; Faculty of Medicine;Urmia University of Medical Sciences; Urmia; Iran. habibmhaji@yahoo.com.
authors.forename: Habib
authors.initials: H
authors.lastname: Mohammadzadeh Hajipirloo
authors.affiliation: Department of Medical Parasitology and Mycology; Faculty of Medicine;Urmia University of Medical Sciences; Urmia; Iran. sa.karamati@yahoo.com.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Keramati
authors.affiliation: Cellular and Molecular Research Center; Urmia University of Medical Sciences, Urmia, Iran and Department of Medical Parasitology and Mycology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran. hazrati_tappeh@yahoo.co.nz.
authors.forename: Khosrow
authors.initials: K
authors.lastname: Hazrati Tappeh
authors.affiliation: Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. arezoobozorgomid@yahoo.com.
authors.forename: Arezoo
authors.initials: A
authors.lastname: Bozorgomid
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D008288:Malaria / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D017780:Malaria Vaccines / Q000276:immunology / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010962:Plasmodium berghei / Q000276:immunology*; D011433:Propranolol / Q000494:pharmacology*; D018417:Th1 Cells / Q000276:immunology / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000319:Adrenergic beta-Antagonists; D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D017780:Malaria Vaccines; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D011433:Propranolol