title: Evaluation of anti-inflammatory activity and fast UHPLC-DAD-IT-TOF profiling of polyphenolic compounds extracted from green lettuce (Lactuca sativa L.; var. Maravilla de Verano).
abstract: Fresh cut vegetables represent a widely consumed food worldwide. Among these, lettuce (Lactuca sativa L.) is one of the most popular on the market. The growing interest for this "healthy" food is related to the content of bioactive compounds, especially polyphenols, that show many beneficial effects. In this study, we report the anti-inflammatory and antioxidant potential of polyphenols extracted from lettuce (var. Maravilla de Verano), in J774A.1 macrophages stimulated with Escherichia coli lipopolysaccharide (LPS). Lettuce extract significantly decreased reactive oxygen species, nitric oxide release, inducible nitric oxide synthase and cycloxygenase-2 expression. A detailed quali/quantitative profiling of the polyphenolic content was carried out, obtaining fast separation (10 min), good retention time and peak area repeatability, (RSD% 0.80 and 8.68, respectively) as well as linearity (R(2)⩾ 0.999) and mass accuracy (⩽ 5 ppm). Our results show the importance in the diet of this cheap and popular food for his healthy properties.
authors.affiliation: Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano, Salerno, Italy.
authors.forename: Giacomo
authors.initials: G
authors.lastname: Pepe
authors.affiliation: Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano, Salerno, Italy.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Sommella
authors.affiliation: Department of Science, University of Basilicata, Viale dell'Ateneo Lucano 10, I-85100 Potenza, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Manfra
authors.affiliation: Pharmaco-Chemical Department, Faculty of Pharmacy, University of Messina, Viale Annunziata, I-98168 Messina, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: De Nisco
authors.affiliation: Department of Pharmacy, University of Napoli Federico II, Via D. Montesano 49, I-80131 Napoli, Italy.
authors.forename: Gian Carlo
authors.initials: GC
authors.lastname: Tenore
authors.affiliation: School of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, Via dell'Ateneo Lucano 10, I-85100 Potenza, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Scopa
authors.affiliation: School of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, Via dell'Ateneo Lucano 10, I-85100 Potenza, Italy.
authors.forename: Adriano
authors.initials: A
authors.lastname: Sofo
authors.affiliation: Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano, Salerno, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Marzocco
authors.affiliation: Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano, Salerno, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Adesso
authors.affiliation: Department of Clinical Medicine and Surgery, University of Napoli Federico II, Via S. Pansini, 5, 80131 Napoli, Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Novellino
authors.affiliation: Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano, Salerno, Italy. Electronic address: pcampiglia@unisa.it.
authors.forename: Pietro
authors.initials: P
authors.lastname: Campiglia
mesh_terms: D000893:Anti-Inflammatory Agents / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism; D018545:Lactuca / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D059808:Polyphenols / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols
keywords: Anti-inflammatory; Lettuce; Polyphenols; UHPLC