title: The role of a potassium transporter OsHAK5 in potassium acquisition and transport from roots to shoots in rice at low potassium supply levels.
abstract: In plants, K transporter (KT)/high affinity K transporter (HAK)/K uptake permease (KUP) is the largest potassium (K) transporter family; however, few of the members have had their physiological functions characterized in planta. Here, we studied OsHAK5 of the KT/HAK/KUP family in rice (Oryza sativa). We determined its cellular and tissue localization and analyzed its functions in rice using both OsHAK5 knockout mutants and overexpression lines in three genetic backgrounds. A β-glucuronidase reporter driven by the OsHAK5 native promoter indicated OsHAK5 expression in various tissue organs from root to seed, abundantly in root epidermis and stele, the vascular tissues, and mesophyll cells. Net K influx rate in roots and K transport from roots to aerial parts were severely impaired by OsHAK5 knockout but increased by OsHAK5 overexpression in 0.1 and 0.3 mm K external solution. The contribution of OsHAK5 to K mobilization within the rice plant was confirmed further by the change of K concentration in the xylem sap and K distribution in the transgenic lines when K was removed completely from the external solution. Overexpression of OsHAK5 increased the K-sodium concentration ratio in the shoots and salt stress tolerance (shoot growth), while knockout of OsHAK5 decreased the K-sodium concentration ratio in the shoots, resulting in sensitivity to salt stress. Taken together, these results demonstrate that OsHAK5 plays a major role in K acquisition by roots faced with low external K and in K upward transport from roots to shoots in K-deficient rice plants.
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.) and Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.), Nanjing Agricultural University, Nanjing 210095, China; andR.H. Smith Institute of Plant Sciences and Genetics in Agriculture, R.H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel (N.M.).
authors.forename: Tianyuan
authors.initials: T
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.) and Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.), Nanjing Agricultural University, Nanjing 210095, China; andR.H. Smith Institute of Plant Sciences and Genetics in Agriculture, R.H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel (N.M.).
authors.forename: Song
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.) and Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.), Nanjing Agricultural University, Nanjing 210095, China; andR.H. Smith Institute of Plant Sciences and Genetics in Agriculture, R.H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel (N.M.).
authors.forename: Yibing
authors.initials: Y
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.) and Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.), Nanjing Agricultural University, Nanjing 210095, China; andR.H. Smith Institute of Plant Sciences and Genetics in Agriculture, R.H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel (N.M.).
authors.forename: Fachi
authors.initials: F
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.) and Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.), Nanjing Agricultural University, Nanjing 210095, China; andR.H. Smith Institute of Plant Sciences and Genetics in Agriculture, R.H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel (N.M.).
authors.forename: Qingdi
authors.initials: Q
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.) and Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.), Nanjing Agricultural University, Nanjing 210095, China; andR.H. Smith Institute of Plant Sciences and Genetics in Agriculture, R.H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel (N.M.).
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.) and Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.), Nanjing Agricultural University, Nanjing 210095, China; andR.H. Smith Institute of Plant Sciences and Genetics in Agriculture, R.H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel (N.M.).
authors.forename: Jing
authors.initials: J
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.) and Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.), Nanjing Agricultural University, Nanjing 210095, China; andR.H. Smith Institute of Plant Sciences and Genetics in Agriculture, R.H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel (N.M.).
authors.forename: Ting
authors.initials: T
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.) and Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.), Nanjing Agricultural University, Nanjing 210095, China; andR.H. Smith Institute of Plant Sciences and Genetics in Agriculture, R.H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel (N.M.).
authors.forename: Nava
authors.initials: N
authors.lastname: Moran
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.) and Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.), Nanjing Agricultural University, Nanjing 210095, China; andR.H. Smith Institute of Plant Sciences and Genetics in Agriculture, R.H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel (N.M.) lyu@njau.edu.cn.
authors.forename: Ling
authors.initials: L
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.) and Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture (T.Y., S.Z., Y.H., F.W., Q.H., G.C., J.C., T.W., L.Y., G.X.), Nanjing Agricultural University, Nanjing 210095, China; andR.H. Smith Institute of Plant Sciences and Genetics in Agriculture, R.H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel (N.M.).
authors.forename: Guohua
authors.initials: G
authors.lastname: Xu
mesh_terms: D017136:Ion Transport; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000502:physiology*; D018517:Plant Roots / Q000378:metabolism*; D018520:Plant Shoots / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011188:Potassium / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D011188:Potassium