title: Plasmonic nanopipette biosensor.
abstract: Integrating a SERS immunoassay on a plasmonic "patch clamp" nanopipette enabled nanobiosensing for the detection of IgG. A SERS response was obtained using a sandwich assay benefiting from plasmon coupling between a capture Au nanoparticle (AuNP) on a nanotip and a second AuNP modified with a Raman active reporter and an antibody selective for IgG. The impact of nanoparticle shape and surface coverage was investigated alongside the choice of Raman active reporter, deposition pH, and plasmonic coupling, in an attempt to fully understand the plasmonic properties of nanopipettes and to optimize the nanobiosensor for the detection of IgG. These probes will find applications in various fields due to their nanoscale size leading to the possibility of spatially and temporally addressing their location near cells to monitor secretion of biomolecules.
authors.affiliation: Department of Chemistry, Université de Montréal , C.P. 6128 Succ. Centre-Ville, Montreal, QC, Canada , H3C 3J7.
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Masson
authors.forename: Julien
authors.initials: J
authors.lastname: Breault-Turcot
authors.forename: Rita
authors.initials: R
authors.lastname: Faid
authors.forename: Hugo-Pierre
authors.initials: HP
authors.lastname: Poirier-Richard
authors.forename: Hélène
authors.initials: H
authors.lastname: Yockell-Lelièvre
authors.forename: Félix
authors.initials: F
authors.lastname: Lussier
authors.forename: Joachim P
authors.initials: JP
authors.lastname: Spatz
mesh_terms: D000906:Antibodies / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D053768:Metal Nanoparticles / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
chemical_list: D000906:Antibodies; D007074:Immunoglobulin G; D006046:Gold