title: Purification and characterization of three antioxidant peptides from protein hydrolyzate of croceine croaker (Pseudosciaena crocea) muscle.
abstract: Three antioxidant peptides were purified from protein hydrolysate of croceine croaker (Pseudosciaena crocea) muscle prepared using pepsin and alcalase, and identified as Tyr-Leu-Met-Ser-Arg (PC-1), Val-Leu-Tyr-Glu-Glu (PC-2), and Met-Ile-Leu-Met-Arg (PC-3) with molecular weights of 651.77, 668.82, and 662.92Da, respectively. PC-1 exhibited the highest scavenging activities on DPPH (EC50 1.35mg/ml), superoxide (EC50 0.450mg/ml), and ABTS (EC50 0.312mg/ml) radicals, but PC-2 exhibited the strongest hydroxyl radical scavenging activity (EC50 0.353mg/ml) among the three peptides. PC-1 also showed effective inhibition on lipid peroxidation in the model system. The good activities of isolated peptides might be benefit from the smaller size and hydrophobic and/or aromatic amino acids within their sequences.
authors.affiliation: School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan, Zhejiang 316000, PR China. Electronic address: chichangfeng@hotmail.com.
authors.forename: Chang-Feng
authors.initials: CF
authors.lastname: Chi
authors.affiliation: School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan, Zhejiang 316000, PR China.
authors.forename: Fa-Yuan
authors.initials: FY
authors.lastname: Hu
authors.affiliation: School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan, Zhejiang 316000, PR China. Electronic address: wangbin4159@hotmail.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan, Zhejiang 316000, PR China.
authors.forename: Xi-Jie
authors.initials: XJ
authors.lastname: Ren
authors.affiliation: School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan, Zhejiang 316000, PR China.
authors.forename: Shang-Gui
authors.initials: SG
authors.lastname: Deng
authors.affiliation: School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan, Zhejiang 316000, PR China.
authors.forename: Chang-Wen
authors.initials: CW
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D015227:Lipid Peroxidation; D013058:Mass Spectrometry; D009132:Muscles / Q000737:chemistry / Q000378:metabolism*; D010434:Pepsin A / Q000378:metabolism; D010455:Peptides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D010473:Perciformes / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D013381:Subtilisins / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010455:Peptides; D011506:Proteins; D013381:Subtilisins; D010434:Pepsin A
keywords: Antioxidant activity; Croceine croaker (Pseudosciaena crocea); Peptide; Protein hydrolysate