title: A molecular mechanism of mitotic centrosome assembly in Drosophila.
abstract: Centrosomes comprise a pair of centrioles surrounded by pericentriolar material (PCM). The PCM expands dramatically as cells enter mitosis, but it is unclear how this occurs. In this study, we show that the centriole protein Asl initiates the recruitment of DSpd-2 and Cnn to mother centrioles; both proteins then assemble into co-dependent scaffold-like structures that spread outwards from the mother centriole and recruit most, if not all, other PCM components. In the absence of either DSpd-2 or Cnn, mitotic PCM assembly is diminished; in the absence of both proteins, it appears to be abolished. We show that DSpd-2 helps incorporate Cnn into the PCM and that Cnn then helps maintain DSpd-2 within the PCM, creating a positive feedback loop that promotes robust PCM expansion around the mother centriole during mitosis. These observations suggest a surprisingly simple mechanism of mitotic PCM assembly in flies.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.
authors.forename: Paul T
authors.initials: PT
authors.lastname: Conduit
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.
authors.forename: Jennifer H
authors.initials: JH
authors.lastname: Richens
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.
authors.forename: Alan
authors.initials: A
authors.lastname: Wainman
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.
authors.forename: James
authors.initials: J
authors.lastname: Holder
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.
authors.forename: Catarina C
authors.initials: CC
authors.lastname: Vicente
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.
authors.forename: Metta B
authors.initials: MB
authors.lastname: Pratt
authors.affiliation: Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom.
authors.forename: Carly I
authors.initials: CI
authors.lastname: Dix
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.
authors.forename: Zsofia A
authors.initials: ZA
authors.lastname: Novak
authors.affiliation: Oxford Micron advanced imaging unit, Department of Biochemistry, University of Oxford, Oxford, United Kingdom.
authors.forename: Ian M
authors.initials: IM
authors.lastname: Dobbie
authors.affiliation: Oxford Micron advanced imaging unit, Department of Biochemistry, University of Oxford, Oxford, United Kingdom.
authors.forename: Lothar
authors.initials: L
authors.lastname: Schermelleh
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.
authors.forename: Jordan W
authors.initials: JW
authors.lastname: Raff
mesh_terms: D000818:Animals; D001665:Binding Sites; D002502:Centrioles / Q000378:metabolism; D018385:Centrosome / Q000378:metabolism*; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology* / Q000378:metabolism*; D005260:Female; D036681:Fluorescence Recovery After Photobleaching; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008938:Mitosis; D008954:Models, Biological; D020798:Two-Hybrid System Techniques
chemical_list: D029721:Drosophila Proteins; D049452:Green Fluorescent Proteins
keywords: D. melanogaster; FRAP; PCM; cell biology; centrosome; centrosome maturation; developmental biology; live super-resolution; stem cells