title: AUF1 is recruited to the stress granules induced by coxsackievirus B3.
abstract: Stress granules (SGs) are cytoplasmic granules that are formed in cells when stress occurs. In this study, we found that SGs formed in cells infected with coxsackievirus B3 (CVB3), evidenced with the co-localization of some accepted SG markers in the viral infection-induced granules. We further discovered that adenosine-uridine (AU)-rich element RNA binding factor 1 (AUF1), which can bind to mRNAs and regulate their translation, was recruited to the SGs in response to high dose of CVB3 by detecting the co-localization of AUF1 with SG markers. Similar results were also observed in the enterovirus 71 (EV71)-infected cells. Finally, we demonstrated that AUF1 was also recruited to arsenite-induced SGs, suggesting that the recruitment of AUF1 to SG is not a specific response to viral infection. In summary, our data indicate that both CVB3 and EV71 infections can induce SG formation, and AUF1 is a novel SG component upon the viral infections. Our findings may shed light on understanding the picornavirus-host interaction.
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin 150081, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin 150081, China.
authors.forename: Lexun
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Cell Biology, Harbin Medical University, Harbin 150081, China.
authors.forename: Wenran
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Pathology, Harbin Medical University, Harbin 150081, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin 150081, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin 150081, China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Si
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin 150081, China.
authors.forename: Tianying
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin 150081, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin 150081, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhai
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin 150081, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhong
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin 150081, China.
authors.forename: Shuoyang
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin 150081, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Tong
authors.affiliation: Department of Microbiology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China. Electronic address: zhikaixu@fmmu.edu.cn.
authors.forename: Zhikai
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin 150081, China. Electronic address: zhongzh@hrbmu.edu.cn.
authors.forename: Zhaohua
authors.initials: Z
authors.lastname: Zhong
mesh_terms: D018053:Arsenites / Q000633:toxicity; D003594:Cytoplasmic Granules / Q000737:chemistry*; D029821:Enterovirus A, Human / Q000502:physiology; D029822:Enterovirus B, Human / Q000502:physiology*; D006367:HeLa Cells; D000081035:Heterogeneous Nuclear Ribonucleoprotein D0; D034502:Heterogeneous-Nuclear Ribonucleoprotein D / Q000032:analysis*; D054884:Host-Pathogen Interactions; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D013312:Stress, Physiological
chemical_list: D018053:Arsenites; C000630986:HNRNPD protein, human; D000081035:Heterogeneous Nuclear Ribonucleoprotein D0; D034502:Heterogeneous-Nuclear Ribonucleoprotein D
keywords: AUF1; Coxsackievirus B3; Enterovirus 71; Picornavirus; Stress granule