title: Chrysanthemum boreale Makino essential oil induces keratinocyte proliferation and skin regeneration.
abstract: We investigated the effect of essential oil from the flower of Chrysanthemum boreale Makino (CBMEO) on growth of human keratinocytes (HaCaTs) and explored a possible mechanism for this response. CBMEO was extracted using the steam distillation method. CBMEO contained a total of 33 compounds. CBMEO stimulated HaCaT proliferation (EC50, 0.028 μg/mL) and also induced phosphorylation of Akt and ERK1/2 in HaCaTs (EC50, 0.007 and 0.005 μg/mL, for phosphorylated Akt and ERK1/2, respectively). Moreover, CBMEO promoted wound closure in the dorsal side skin of rat tail. This study demonstrated that CBMEO can stimulate growth of human skin keratinocytes, probably through the Akt and ERK1/2 pathways. Therefore, CBMEO may be helpful in skin regeneration and wound healing in human skin, and may also be a possible cosmetic material for skin beauty.
authors.affiliation: a Department of Cosmetic Science , College of Natural Science, Hoseo University , Asan-City , Chungnam Prefecture 336-795 , Republic of Korea.
authors.forename: Do Yoon
authors.initials: DY
authors.lastname: Kim
authors.forename: Kyung-Jong
authors.initials: KJ
authors.lastname: Won
authors.forename: Mi-So
authors.initials: MS
authors.lastname: Yoon
authors.forename: Dae Il
authors.initials: DI
authors.lastname: Hwang
authors.forename: Seok Won
authors.initials: SW
authors.lastname: Yoon
authors.forename: Joo-Hoon
authors.initials: JH
authors.lastname: Park
authors.forename: Bokyung
authors.initials: B
authors.lastname: Kim
authors.forename: Hwan Myung
authors.initials: HM
authors.lastname: Lee
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D031202:Chrysanthemum / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009822:Oils, Volatile / Q000494:pharmacology*; D010938:Plant Oils / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D012038:Regeneration / Q000187:drug effects*; D012867:Skin / Q000187:drug effects*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D009822:Oils, Volatile; D010938:Plant Oils; D051057:Proto-Oncogene Proteins c-akt; D048052:Mitogen-Activated Protein Kinase 3
keywords: Chrysanthemum boreale Makino; essential oil; keratinocyte; skin; wound healing