title: Cytotoxic Effects of Newly Synthesized Palladium(II) Complexes of Diethyldithiocarbamate on Gastrointestinal Cancer Cell Lines.
abstract: As a part of a drug development program to discover novel therapeutic and more effective palladium (Pd) based anticancer drugs, a series of water-soluble Pd complexes have been synthesized by interaction between [Pd (phen)(H2O)2(NO3)2] and alkylenebisdithiocarbamate(al-bis-dtc) disodium salts. This study was undertaken to examine the possible cytotoxic effect of three novel complexes (0.125-64 µg/mL) on human gastric carcinoma (AGS), esophageal squamous cell carcinoma (Kyse-30), and hepatocellular carcinoma (HepG2) cell lines. The cytotoxicity was examined using cell proliferation and acridine orange/ethidium bromide (AO/EB) assay. In order to examine the effects of new Pd(II) complexes on cell cycle status, we performed cell cycle analysis. The complexes were found to have completely lethal effects on the cell lines, and the half maximal inhibitory concentration (IC50) values obtained for the cell lines were much lower in comparison with cisplatin. We demonstrated that the three new Pd(II) complexes are able to induce G2/M phase arrest in AGS and HepG2; in addition, the Pd(II) complexes caused an S phase arrest in Kyse-30 cell line. Our results indicate that newly synthesized Pd(II) complexes may provide a novel class of chemopreventive compounds for anticancer therapy.
authors.affiliation: Department of Biochemistry, School of Medicine, Ardabil University of Medical Sciences, Ardabil 5618985991, Iran.
authors.forename: Shahram
authors.initials: S
authors.lastname: Hadizadeh
authors.affiliation: Laboratory of Embryology and Stem Cells, Department of Anatomy and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil 5618985991, Iran.
authors.forename: Nowruz
authors.initials: N
authors.lastname: Najafzadeh
authors.affiliation: Department of Biochemistry, School of Medicine, Ardabil University of Medical Sciences, Ardabil 5618985991, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Mazani
authors.affiliation: Department of Biophysics, School of Medicine, Ardabil University of Medical Sciences, Ardabil 5618985991, Iran.
authors.forename: Mojtaba
authors.identifier: 0000-0003-2666-7519
authors.initials: M
authors.lastname: Amani
authors.affiliation: Department of Chemistry, Faculty of Basic Sciences, University of Sistan and Baluchistan, Zahedan 9816745345, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Mansouri-Torshizi
authors.affiliation: Laboratory of Embryology and Stem Cells, Department of Anatomy and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil 5618985991, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Niapour