title: The interaction between Shroom3 and Rho-kinase is required for neural tube morphogenesis in mice.
abstract: Shroom3 is an actin-associated regulator of cell morphology that is required for neural tube closure, formation of the lens placode, and gut morphogenesis in mice and has been linked to chronic kidney disease and directional heart looping in humans. Numerous studies have shown that Shroom3 likely regulates these developmental processes by directly binding to Rho-kinase and facilitating the assembly of apically positioned contractile actomyosin networks. We have characterized the molecular basis for the neural tube defects caused by an ENU-induced mutation that results in an arginine-to-cysteine amino acid substitution at position 1838 of mouse Shroom3. We show that this substitution has no effect on Shroom3 expression or localization but ablates Rock binding and renders Shroom3 non-functional for the ability to regulate cell morphology. Our results indicate that Rock is the major downstream effector of Shroom3 in the process of neural tube morphogenesis. Based on sequence conservation and biochemical analysis, we predict that the Shroom-Rock interaction is highly conserved across animal evolution and represents a signaling module that is utilized in a variety of biological processes.
authors.affiliation: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA.
authors.forename: Debamitra
authors.initials: D
authors.lastname: Das
authors.affiliation: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA.
authors.forename: Jenna K
authors.initials: JK
authors.lastname: Zalewski
authors.affiliation: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA.
authors.forename: Swarna
authors.initials: S
authors.lastname: Mohan
authors.affiliation: College of Optometry, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Timothy F
authors.initials: TF
authors.lastname: Plageman
authors.affiliation: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA jeffh@pitt.edu andyv@pitt.edu.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: VanDemark
authors.affiliation: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA jeffh@pitt.edu andyv@pitt.edu.
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Hildebrand
keywords: Rock; Shroom3; apical constriction; epithelial; neural tube