title: Intravital imaging of cytotoxic T lymphocytes.
abstract: Intravital imaging approaches are proving to be essential to address new questions to better understand how the immune system operates. These approaches are especially valuable to characterize the complex organization of immune responses in vivo. Here, we examine how to take advantage of the cornea as a natural body window to apply noninvasive imaging techniques to assess cytotoxic T lymphocyte involvement in the immune rejection process in a model of intraocular allogeneic islet transplantation.
authors.affiliation: Transplantation Research Laboratory, Department of Surgery, University of California, 513 Parnassus Ave., HSE-520, San Francisco, CA, 94143, USA, Gaetano.Faleo@ucsfmedctr.org.
authors.forename: Gaetano
authors.initials: G
authors.lastname: Faleo
authors.forename: Per-Olof
authors.initials: PO
authors.lastname: Berggren
authors.forename: Antonello
authors.initials: A
authors.lastname: Pileggi
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000276:immunology*; D003315:Cornea / Q000502:physiology; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D006084:Graft Rejection / Q000276:immunology*; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D051379:Mice; D008811:Mice, Inbred DBA; D008853:Microscopy / Q000379:methods*; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology*; D014184:Transplantation, Homologous