title: Functional lysophosphatidic acid receptors expressed in Oryzias latipes.
abstract: Lysophosphatidic acid (LPA) signaling is known to play biological and pathophysiological roles in many types of animals. Medaka (Oryzias latipes) is an experimental fish that can be easily maintained, propagated, and analyzed, and whose genome has been completely sequenced. However, there is limited information available regarding medaka LPA receptors. Here, using information from the medaka genome database, we examine the genomic structures, expression, and functions of six LPA receptor genes, Lpar1-Lpar6. Our analyses reveal that the genomic structures of Lpar1 and Lpar4 are different from those deduced from the database. Functional analyses using a heterologous expression system demonstrate that all medaka LPA receptors except for LPA5b respond to LPA treatment with cytoskeletal changes. These findings provide useful information on the structure and function of medaka LPA receptor genes, and identify medaka as a useful experimental model for exploration of the biological significance of LPA signaling.
authors.affiliation: Division of Molecular Neurobiology, Department of Life Science, Kinki University, Higashiosaka, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Morimoto
authors.affiliation: Division of Molecular Neurobiology, Department of Life Science, Kinki University, Higashiosaka, Japan.
authors.forename: Shoichi
authors.initials: S
authors.lastname: Ishii
authors.affiliation: Division of Molecular Neurobiology, Department of Life Science, Kinki University, Higashiosaka, Japan.
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Ishibashi
authors.affiliation: Immunology Frontier Research Center, Osaka University, Suita, Osaka, Japan; Computational Biology Research Center, The National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan.
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Katoh
authors.affiliation: Division of Cancer Biology and Bioinformatics, Department of Life Science, Kinki University, Higashiosaka, Japan.
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Tsujiuchi
authors.affiliation: Division of Animal Genetics, Department of Life Science, Kinki University, Higashiosaka, Japan.
authors.forename: Nao
authors.initials: N
authors.lastname: Kagawa
authors.affiliation: Division of Molecular Neurobiology, Department of Life Science, Kinki University, Higashiosaka, Japan. Electronic address: nfukushima@life.kindai.ac.jp.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Fukushima
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D002874:Chromosome Mapping; D018387:Codon, Initiator / Q000235:genetics; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D005091:Exons / Q000235:genetics; D005260:Female; D029941:Fish Proteins / Q000235:genetics*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008246:Lysophospholipids / Q000494:pharmacology; D008297:Male; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D009990:Oryzias / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics; D049368:Receptors, Lysophosphatidic Acid / Q000145:classification / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000199:Actins; D018387:Codon, Initiator; D029941:Fish Proteins; D008246:Lysophospholipids; D020033:Protein Isoforms; D049368:Receptors, Lysophosphatidic Acid; C032881:lysophosphatidic acid
keywords: Expression; Genomic structure; Oryzias latipes; lysophosphatidic acid receptor