title: Distribution of H-FABP and ACSL4 gene polymorphisms and their associations with intramuscular fat content and backfat thickness in different pig populations.
abstract: Here, we analyzed the distribution of H-FABP/(HinfI, MspI, and HaeIII) and ACSL4/RsaI polymorphisms, and the associations of these 4 polymorphic loci with intramuscular fat (IMF) content and backfat thickness (BFT) in Yanan, Jinhua, Duroc, Landrace, Yorkshire, and Duroc x (Landrace x Yorkshire) (DLY) pigs. H-FABP/HinfI polymorphisms were present in all the 6 populations. At the ACSL4/RsaI locus, sows had 3 genotypes, whereas boars only had haplotype A or G, in Duroc, Landrace, Yorkshire, and DLY pigs. H-FABP/(MspI and HaeIII) and ACSL4/RsaI polymorphisms were absent in Yanan and Jinhua pigs. Linkage disequilibrium analysis indicated that the 3 loci (HinfI, MspI, and HaeIII) were separated. Association analysis showed that the H-FABP/HinfI locus significantly affected IMF content in DLY (P < 0.05) and Yanan (P < 0.001) pigs. The highest IMF content was recorded in the adH haplotype of the 3 H-FABP polymorphic loci (2.59%, P < 0.05) in DLY pigs. At the ACSL4/RsaI locus, higher IMF content was recorded for sows with a GG genotype or boars with a G haplotype compared to those with an AA genotype (2.53 vs 2.10%, P < 0.05) or A haplotype (2.48 vs 1.73%, P < 0.01) in DLY pigs. Significant differences were not obtained among these 4 polymorphic loci and BFT (P > 0.05). The results indicate that H-FABP and ACSL4 genes might serve as markers to improve IMF content (but not BFT) in the pig breeding system.
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an, China.
authors.forename: J N
authors.initials: JN
authors.lastname: Chen
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an, China jiangyz04@163.com.
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Jiang
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an, China.
authors.forename: W M
authors.initials: WM
authors.lastname: Cen
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an, China.
authors.forename: S H
authors.initials: SH
authors.lastname: Xing
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Tang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: M Z
authors.initials: MZ
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: A A
authors.initials: AA
authors.lastname: Jiang
authors.affiliation: The Zhejiang Jinhua Pig Farm, Jinhuan, China.
authors.forename: P E
authors.initials: PE
authors.lastname: Lou
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an, China.
authors.forename: A X
authors.initials: AX
authors.lastname: Wen
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an, China.
authors.forename: T
authors.initials: T
authors.lastname: He
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an, China.
authors.forename: G X
authors.initials: GX
authors.lastname: Zhu
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an, China.
authors.forename: M
authors.initials: M
authors.lastname: Xie
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: X W
authors.initials: XW
authors.lastname: Li
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000483:Alleles; D000818:Animals; D003066:Coenzyme A Ligases / Q000235:genetics*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005828:Genetics, Population / Q000379:methods; D005838:Genotype; D006239:Haplotypes; D015810:Linkage Disequilibrium; D008297:Male; D009132:Muscles / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D013552:Swine
chemical_list: D050556:Fatty Acid-Binding Proteins; D003066:Coenzyme A Ligases