title: High performance liquid chromatographic determination of ultra traces of two tricyclic antidepressant drugs imipramine and trimipramine in urine samples after their dispersive liquid-liquid microextraction coupled with response surface optimization.
abstract: Dispersive liquid-liquid microextraction (DLLME) coupled with high performance liquid chromatography by ultraviolet detection (HPLC-UV) as a fast and inexpensive technique was applied to the determination of imipramine and trimipramine in urine samples. Response surface methodology (RSM) was used for multivariate optimization of the effects of seven different parameters influencing the extraction efficiency of the proposed method. Under optimized experimental conditions, the enrichment factors and extraction recoveries were between 161.7-186.7 and 97-112%, respectively. The linear range and limit of detection for both analytes found to be 5-100ng mL(-1) and 0.6ng mL(-1), respectively. The relative standard deviations for 5ng mL(-1) of the drugs in urine samples were in the range of 5.1-6.1 (n=5). The developed method was successfully applied to real urine sample analyses.
authors.affiliation: Department of Chemistry, Razi University, Kermanshah, Iran. Electronic address: mshamsipur@yahoo.com.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Shamsipur
authors.affiliation: Department of Chemistry, Razi University, Kermanshah, Iran.
authors.forename: Mehrosadat
authors.initials: M
authors.lastname: Mirmohammadi
mesh_terms: D000929:Antidepressive Agents, Tricyclic / Q000493:pharmacokinetics / Q000652:urine*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D006801:Humans; D007099:Imipramine / Q000493:pharmacokinetics / Q000652:urine*; D057230:Limit of Detection; D016014:Linear Models; D059627:Liquid Phase Microextraction / Q000592:standards; D008297:Male; D015233:Models, Statistical; D015999:Multivariate Analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D014299:Trimipramine / Q000493:pharmacokinetics / Q000652:urine*
chemical_list: D000929:Antidepressive Agents, Tricyclic; D012997:Solvents; D014299:Trimipramine; D007099:Imipramine
keywords: Dispersive liquid–liquid microextraction; High-performance liquid chromatography–ultraviolet detection; Response surface methodology; Tricyclic antidepressants; Urine analysis