title: Bioanalysis of (1R,4R,5S,6R)-4-amino-2-oxabicyclo[3.1.0]hexane-4,6-dicarboxylic acid (LY379268) in rat plasma using derivatization liquid chromatography/mass spectrometry.
abstract: BACKGROUND
  (1R,4R,5S,6R)-4-amino-2-oxabicyclo[3.1.0]hexane-4,6-dicarboxylic acid, also known as LY379268, a group II metabotropic glutamate receptor agonist, has been widely used in neuroscience as a model compound in studies evaluating antipsychotic drugs for the treatment of schizophrenia.
  
  
  METHODS
  So far, no reports describing methods of the bioanalysis of LY379268 have been published. Here, a novel method is presented for determining LY379268 in rat plasma employing precolumn derivatization with pentafluorobenzoyl chloride reagent coupled to liquid chromatography/mass spectrometry.
  
  
  CONCLUSIONS
  Chemical derivatization of a low-molecular-weight and highly polar molecule yields a derivative that is retained on a reversed-phase liquid chromatography column with improved tandem mass spectrometric response.
authors.affiliation: Bristol-Myers Squibb Company, Research & Development, Pharmaceutical Candidate Optimization, Bioanalytical Research, 5 Research Parkway, Wallingford, CT 06492, USA.
authors.forename: Yulia
authors.initials: Y
authors.lastname: Benitex
authors.forename: Fu-Ni
authors.initials: FN
authors.lastname: Luan
authors.forename: Eric
authors.initials: E
authors.lastname: Shields
authors.forename: Colleen A
authors.initials: CA
authors.lastname: McNaney
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Morgan
authors.forename: Timothy V
authors.initials: TV
authors.lastname: Olah
authors.forename: Dieter M
authors.initials: DM
authors.lastname: Drexler
mesh_terms: D000596:Amino Acids / Q000097:blood* / Q000737:chemistry; D000818:Animals; D001565:Benzoates / Q000737:chemistry; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000097:blood* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D057230:Limit of Detection; D051381:Rats; D018094:Receptors, Metabotropic Glutamate / Q000819:agonists*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D005464:Fluorobenzenes; D005466:Fluorocarbons
chemical_list: D000596:Amino Acids; D001565:Benzoates; D019086:Bridged Bicyclo Compounds, Heterocyclic; D018094:Receptors, Metabotropic Glutamate; C118218:LY 379268; C014737:pentafluorobenzoyl chloride; D005464:Fluorobenzenes; D005466:Fluorocarbons