title: A new cell line from the fat body of Spodoptera litura (Lepidoptera, Noctuidae) and detection of lysozyme activity release upon immune stimulation.
abstract: A new cell line, designated NIAS-SL64, was established from the fat body of the fifth instar larvae of the common cutworm Spodoptera litura. NIAS-SL64 cells grew as spindle-shaped and non-adherent cells in the insect-specific cell culture medium MGM-450 supplemented with 10% fetal bovine serum. Criterions for the establishment of the NIAS-SL64 cell line is spindle shape and length (30~90 μm) stabilized after 100 passages. The doubling time of the cells was 24 h at 25°C. Lipopolysaccharide significantly stimulated the release of lysozyme activity by NIAS-SL64 cells. Lysozyme is one of the components of the innate immunity and plays important role as lytic enzyme in infection. Lysozyme activity released from NIAS-SL64 would be a marker for immune response. The released lysozyme activity critically depends on morphology of the cells and would be a criterion of the establishment of the cell line. Lysozyme activity was suppressed in a dose-dependent manner by the immunosuppressive agent cyclosporin A.
authors.affiliation: National Institute of Agrobiological Sciences, 2-1-2 Kan-non dai, Tsukuba, Ibaraki, 305-8602, Japan, tateishi@affrc.go.jp.
authors.forename: Ken
authors.initials: K
authors.lastname: Tateishi
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kasahara
authors.forename: Kazuyo
authors.initials: K
authors.lastname: Watanabe
authors.forename: Nobuo
authors.initials: N
authors.lastname: Hosokawa
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Doi
authors.forename: Kaori
authors.initials: K
authors.lastname: Nakajima
authors.forename: Hayamitsu
authors.initials: H
authors.lastname: Adachi
authors.forename: Akio
authors.initials: A
authors.lastname: Nomoto
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D016572:Cyclosporine / Q000494:pharmacology; D005216:Fat Body / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009113:Muramidase / Q000378:metabolism*; D018411:Spodoptera / Q000166:cytology*
chemical_list: D008070:Lipopolysaccharides; D016572:Cyclosporine; D009113:Muramidase