title: Poly(oligoethylene glycol methacrylate) dip-coating: turning cellulose paper into a protein-repellent platform for biosensors.
abstract: The passivation of nonspecific protein adsorption to paper is a major barrier to the use of paper as a platform for microfluidic bioassays. Herein we describe a simple, scalable protocol based on adsorption and cross-linking of poly(oligoethylene glycol methacrylate) (POEGMA) derivatives that reduces nonspecific adsorption of a range of proteins to filter paper by at least 1 order of magnitude without significantly changing the fiber morphology or paper macroporosity. A lateral-flow test strip coated with POEGMA facilitates effective protein transport while also confining the colorimetric reporting signal for easier detection, giving improved performance relative to bovine serum albumin (BSA)-blocked paper. Enzyme-linked immunosorbent assays based on POEGMA-coated paper also achieve lower blank values, higher sensitivities, and lower detection limits relative to ones based on paper blocked with BSA or skim milk. We anticipate that POEGMA-coated paper can function as a platform for the design of portable, disposable, and low-cost paper-based biosensors.
authors.affiliation: Department of Chemical Engineering, McMaster University , Hamilton, Ontario L8S 4L7, Canada.
authors.forename: Xudong
authors.initials: X
authors.lastname: Deng
authors.forename: Niels M B
authors.initials: NM
authors.lastname: Smeets
authors.forename: Clémence
authors.initials: C
authors.lastname: Sicard
authors.forename: Jingyun
authors.initials: J
authors.lastname: Wang
authors.forename: John D
authors.initials: JD
authors.lastname: Brennan
authors.forename: Carlos D M
authors.initials: CD
authors.lastname: Filipe
authors.forename: Todd
authors.initials: T
authors.lastname: Hoare
mesh_terms: D000327:Adsorption; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation; D002417:Cattle; D002482:Cellulose / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation; D004867:Equipment Design; D008689:Methacrylates / Q000737:chemistry*; D010209:Paper; D011092:Polyethylene Glycols / Q000737:chemistry*; D016062:Porosity; D011506:Proteins / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification
chemical_list: D008689:Methacrylates; D011506:Proteins; C524499:polyethylene glycol methacrylate; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D002482:Cellulose