title: Molecular cloning, sequence analysis, prokaryotic expression, and function prediction of foot-specific peroxidase in Hydra magnipapillata Chinese strain.
abstract: The cDNA sequence of foot-specific peroxidase PPOD1 from the Chinese strain of Hydra magnipapillata was cloned by reverse transcription-polymerase chain reaction. The cDNA sequence contained a coding region with an 873-bp open reading frame, a 31-bp 5'-untranslated region, and a 36-bp 3'-untranslated region. The structure prediction results showed that PPOD1 contains 10.34% of α-helix, 38.62% of extended strand, 12.41% of β-turn, and 38.62% of random coil. The structural core was α-helix at the N terminus. The GenBank protein blast server showed that PPOD1 contains 2 fascin-like domains. In addition, high-level PPOD1 activity was only present in the ectodermal epithelial cells located on the edge of the adhesive face of the basal disc, and that these cells extended lamellipodia and filopodia when the basal disc was tightly attached to a glass slide. The fascin-like domains of Hydra PPOD1 might contribute to the bundling of the actin filament of these cells, and hence, the formation of filopodia. In conclusion, these cells might play an important role in strengthening the adsorbability of the basal disc to substrates.
authors.affiliation: College of Life Science, Anhui Normal University, Wuhu, Anhui, China panhongchun@126.com.
authors.forename: H C
authors.initials: HC
authors.lastname: Pan
authors.affiliation: College of Life Science, Anhui Normal University, Wuhu, Anhui, China.
authors.forename: H Q
authors.initials: HQ
authors.lastname: Yang
authors.affiliation: College of Life Science, Anhui Normal University, Wuhu, Anhui, China.
authors.forename: F X
authors.initials: FX
authors.lastname: Zhao
authors.affiliation: College of Life Science, Anhui Normal University, Wuhu, Anhui, China.
authors.forename: X C
authors.initials: XC
authors.lastname: Qian
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002681:China; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D006829:Hydra / Q000201:enzymology / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D009195:Peroxidase / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D011387:Prokaryotic Cells / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011554:Pseudopodia / Q000201:enzymology / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D011994:Recombinant Proteins; D009195:Peroxidase