title: Determining mycotoxins in baby foods and animal feeds using stable isotope dilution and liquid chromatography tandem mass spectrometry.
abstract: We developed a stable isotope dilution assay with liquid chromatography tandem mass spectrometry (LC-MS/MS) to determine multiple mycotoxins in baby foods and animal feeds. Samples were fortified with [(13)C]-uniformly labeled mycotoxins as internal standards ([(13)C]-IS) and prepared by solvent extraction (50% acetonitrile in water) and filtration, followed by LC-MS/MS analysis. Mycotoxins in each sample were quantitated with the corresponding [(13)C]-IS. In general, recoveries of aflatoxins (2-100 ng/g), deoxynivalenol, fumonisins (50-2000 ng/g), ochratoxin A (20-1000 ng/kg), T-2 toxin, and zearalenone (40-2000 ng/g) in tested matrices (grain/rice/oatmeal-based formula, animal feed, dry cat/dog food) ranged from 70 to 120% with relative standard deviations (RSDs) <20%. The method provides sufficient selectivity, sensitivity, accuracy, and reproducibility to screen for aflatoxins at ng/g concentrations and deoxynivalenol and fumonisins at low μg/g concentrations in baby foods and animal feeds, without using conventional standard addition or matrix-matched calibration standards to correct for matrix effects.
authors.affiliation: Office of Regulatory Science, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration , HFS-706, 5100 Paint Branch Parkway, College Park, Maryland 20740, United States.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.forename: Jon W
authors.initials: JW
authors.lastname: Wong
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: Krynitsky
authors.forename: Mary W
authors.initials: MW
authors.lastname: Trucksess
mesh_terms: D000348:Aflatoxins / Q000032:analysis; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002247:Carbon Isotopes; D002415:Cats; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D037341:Fumonisins / Q000032:analysis; D006801:Humans; D007201:Indicator Dilution Techniques; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D009183:Mycotoxins / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis; D015203:Reproducibility of Results; D013605:T-2 Toxin / Q000032:analysis; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014255:Trichothecenes / Q000032:analysis
chemical_list: D000348:Aflatoxins; D002247:Carbon Isotopes; D037341:Fumonisins; D009183:Mycotoxins; D009793:Ochratoxins; D014255:Trichothecenes; C025589:ochratoxin A; D013605:T-2 Toxin; C007262:deoxynivalenol
keywords: LC−MS/MS; animal feeds; baby foods; mycotoxins; stable isotope dilution