title: Exercise stress testing enhances blood coagulation and impairs fibrinolysis in asymptomatic aortic valve stenosis.
abstract: BACKGROUND
  Increased thrombin formation and fibrin deposition on the valves were observed in patients with severe aortic valve stenosis (AS). Exercise enhances blood coagulation and fibrinolysis in healthy subjects, but its haemostatic effects in AS are unknown. We sought to investigate how stress echocardiography alters blood coagulation and fibrinolysis in AS patients free of significant atherosclerotic vascular disease.
  
  
  METHODS
  We studied 32 consecutive asymptomatic moderate-to-severe AS patients and 32 age- and sex-matched controls. We measured peak thrombin generated using calibrated automated thrombogram, clot lysis time (CLT), and fibrinolytic markers at four time points, i.e. at rest, at peak exercise, and 1h and 24h after a symptom-limited exercise test.
  
  
  RESULTS
  We observed that peak thrombin generated rose at peak exercise to 25% higher value in the patients than in controls (p<0.001) and reached its highest level 24h from exercise in AS patients while it decreased post-exercise in controls. Baseline CLT was 13% longer in AS patients (p=0.006) and associated with thrombin activatable fibrinolysis inhibitor (TAFI) activity (r=0.69, p<0.001), antiplasmin (r=0.47, p=0.007), and plasminogen (r=-0.55, p=0.001). In AS, CLT remained unaltered at peak exercise, while it decreased in controls, yielding the intergroup difference of 28% (p<0.001). Twenty-four hours after exercise CLT became 15% longer in AS patients (p<0.001). This hypofibrinolytic effect followed a similar pattern observed for TAFI activity.
  
  
  CONCLUSIONS
  Asymptomatic moderate-to-severe AS patients respond to exercise with more pronounced and prolonged increase in thrombin generation, together with impaired fibrinolysis as compared to controls. Repeated episodes of exercise-induced prothrombotic state in AS might contribute to the progression of this disease.
authors.affiliation: John Paul II Hospital, Krakow, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Kolasa-Trela
authors.affiliation: John Paul II Hospital, Krakow, Poland.
authors.forename: Korneliusz
authors.initials: K
authors.lastname: Fil
authors.affiliation: John Paul II Hospital, Krakow, Poland; Institute of Cardiology, Jagiellonian University Medical College, Krakow, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Wypasek
authors.affiliation: John Paul II Hospital, Krakow, Poland; Institute of Cardiology, Jagiellonian University Medical College, Krakow, Poland. Electronic address: mmundas@cyf-kr.edu.pl.
authors.forename: Anetta
authors.initials: A
authors.lastname: Undas
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001024:Aortic Valve Stenosis / Q000097:blood / Q000503:physiopathology*; D015415:Biomarkers / Q000097:blood; D001777:Blood Coagulation / Q000502:physiology*; D025901:Carboxypeptidase B2 / Q000097:blood; D016022:Case-Control Studies; D005080:Exercise Test; D005260:Female; D063285:Fibrin Clot Lysis Time; D005342:Fibrinolysis / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D013917:Thrombin / Q000096:biosynthesis
chemical_list: D015415:Biomarkers; D025901:Carboxypeptidase B2; D013917:Thrombin
keywords: Aortic valve stenosis; Exercise; Fibrinolysis; Thrombin potential