title: Molybdenum disulfide (MoS2) nanoflakes as inherently electroactive labels for DNA hybridization detection.
abstract: The detection of specific DNA sequences plays a critical role in the areas of medical diagnostics, environmental monitoring, drug discovery and food safety. This has therefore become a strong driving force behind the ever-increasing demand for simple, cost-effective, highly sensitive and selective DNA biosensors. In this study, we report for the first time, a novel approach for the utilization of molybdenum disulfide nanoflakes, a member of the transition metal dichalcogenides family, in the detection of DNA hybridization. Herein, molybdenum disulfide nanoflakes serve as inherently electroactive labels, with the inherent oxidation peak exploited as the analytical signal. The principle of detection is based on the differential affinity of molybdenum disulfide nanoflakes towards single-stranded DNA and double-stranded DNA. The employment of transition metal dichalcogenide nanomaterials for sensing and biosensing purposes represents an upcoming research area which holds great promise. Hence, our findings are anticipated to have significant contributions towards the fabrication of future DNA biosensors.
authors.affiliation: Division of Chemistry & Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore. a.bonanni@ntu.edu.sg pumera@ntu.edu.sg pumera.research@outlook.com.
authors.forename: Adeline Huiling
authors.initials: AH
authors.lastname: Loo
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bonanni
authors.forename: Adriano
authors.initials: A
authors.lastname: Ambrosi
authors.forename: Martin
authors.initials: M
authors.lastname: Pumera
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D017097:Electric Impedance; D004563:Electrochemistry; D006108:Graphite; D008982:Molybdenum / Q000737:chemistry*; D050997:Nanomedicine; D053758:Nanoparticles / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010100:Oxygen / Q000737:chemistry
chemical_list: D004277:DNA, Single-Stranded; D004220:Disulfides; D006108:Graphite; D008982:Molybdenum; D004247:DNA; D010100:Oxygen; C082964:molybdenum disulfide