title: Does Omegaven have beneficial effects on a rat model of ovarian ischemia/reperfusion?
abstract: OBJECTIVE
  The beneficial effects of omega-3 fatty acids on an intestinal ischemia/reperfusion (I/R) model was shown previously. Therefore, we aimed to examine the potential beneficial effects of parenteral omega-3 fatty acids, a safe and inexpensive product, on a rat model of ovarian I/R.
  
  
  METHODS
  A group of 39 rats was divided into six groups. Group 1 (Sham Group; n=6) underwent two laparotomies with a 3-h interval and their ovaries were removed 3h later. Group 2 (torsion-detorsion Group; n=7) had their ovaries torsioned clockwise and fixed at 720°; 3h later a detorsion operation was done and after another 3h, their ovaries were removed. Group 3 (n=7) and Group 4 (n=7) received the same treatment as Group 2; however, half an hour prior to detorsion, these rats received Omegaven at 1mL/kg and 5mL/kg, respectively. Group 5 (n=6) and Group 6 (n=6) received the same treatment as Group 1; however, half an hour prior to the second laparotomy, these rats received Omegaven at 1mL/kg and 5mL/kg, respectively. One ovary from each rat was evaluated histologically by hematoxylin and eosin (H&E) staining and the other ovary was homogenized and evaluated for total oxidant status (TOS), total antioxidant status (TAS) and oxidative stress index (OSI).
  
  
  RESULTS
  While we failed to show any significant relationship among groups in oxidative parameters, there was a significant worsening in the torsion-detorsion group in histological evaluation. High Omegaven doses, but not low doses, improved tissue injury scores of torsioned and detorsioned ovaries to the levels observed in the control group.
  
  
  CONCLUSIONS
  Omegaven improves the detrimental effects of ovarian I/R when used in sufficient doses. Its effects and dose adjustment on women with ovarian torsion must be investigated by further studies.
authors.affiliation: Canakkale Onsekiz Mart University, Faculty of Medicine, Department of Obstetrics and Gynecology, Canakkale, Turkey. Electronic address: dr_aysecakir@hotmail.com.
authors.forename: Ayse N Cakir
authors.initials: AN
authors.lastname: Gungor
authors.affiliation: Canakkale Onsekiz Mart University, Faculty of Medicine, Department of Biochemistry, Canakkale, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Turkon
authors.affiliation: Diskapi Education and Research Hospital, Department of Pathology, Ankara, Turkey.
authors.forename: Aynur
authors.initials: A
authors.lastname: Albayrak
authors.affiliation: Canakkale Onsekiz Mart University, Faculty of Medicine, Department of Physiology, Canakkale, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ovali
authors.affiliation: Balikesir University, Faculty of Medicine, Department of Obstetrics and Gynecology, Balikesir, Turkey.
authors.forename: Mine
authors.initials: M
authors.lastname: Islimye
authors.affiliation: Canakkale Onsekiz Mart University, Faculty of Medicine, Department of Obstetrics and Gynecology, Canakkale, Turkey.
authors.forename: Meryem
authors.initials: M
authors.lastname: Gencer
authors.affiliation: Canakkale Onsekiz Mart University, Faculty of Medicine, Department of Obstetrics and Gynecology, Canakkale, Turkey.
authors.forename: Servet
authors.initials: S
authors.lastname: Hacivelioglu
authors.affiliation: Canakkale Onsekiz Mart University, Faculty of Medicine, Department of Public Health, Canakkale, Turkey.
authors.forename: Sibel
authors.initials: S
authors.lastname: Cevizci
authors.affiliation: Canakkale Onsekiz Mart University, Faculty of Medicine, Department of Pathology, Canakkale, Turkey.
authors.forename: Ismet
authors.initials: I
authors.lastname: Cesur
authors.affiliation: Canakkale Onsekiz Mart University, Faculty of Medicine, Department of Obstetrics and Gynecology, Canakkale, Turkey.
authors.forename: Emine
authors.initials: E
authors.lastname: Cosar
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage*; D007274:Injections, Intraperitoneal; D010049:Ovarian Diseases / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D010053:Ovary / Q000098:blood supply / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D014102:Torsion Abnormality / Q000150:complications*; D014280:Triglycerides
chemical_list: D005395:Fish Oils; D014280:Triglycerides; C568345:fish oil triglycerides
keywords: Ischemia/reperfusion injury; Omega-3 fatty acids; Ovarian torsion; Rat