title: [Role of vascular cell adhesion molecule-1 in the mouse model of hepatic ischemia/reperfusion and the hematogenic metastasis].
abstract: OBJECTIVE
  To investigate the effect of ischemia/reperfusion (I/R) on tumor metastasis in a experimental mouse model of hematogenous metastasis after I/R and to quantify expression of vascular cell adhesion molecule-1 (VCAM-1) during I/R.
  
  
  METHODS
  An experimental mouse model of metastasis after partial hepatic I/R was designed to determine the effects of I/R on tumor metastasis to liver. Tumor loads were valued 14 days after operation. In addition, the expressions of alanine transaminase (ALT), aspartate transaminase (AST), and VCAM-1 were detected.
  
  
  RESULTS
  Two hours after hepatic reperfusion, ALT and AST levels in ischemia 45-minute group and ischemia 30-minute group were significantly higher than in the sham group (all P < 0.05). Also, the changes of ALT and AST were more obvious in the ischemia 45-minute group than in ischemia 30-minute group (all P < 0.05). In the sham group, both ALT and AST slightly and transiently increased. ALT and AST in the ischemia 45-minute group and ischemia 30-minute group at 8 hours were both significantly higher than those at 2 hours reperfusion (P<0.05). The tumor load (valued by hepatic replacement area) and the expression of VCAM-1 in ischemic lobe were significantly larger in the ischemia 45-minute group than in the ischemia 30-minute group and sham group (P = 0.013, P = 0.007). However, there was no statistical difference on tumor load between the right lobe of sham operated mice and the right lobe (nonischemic lobes) of mice subjected to I/R (P = 0.089). Mouse survivals were significantly longer in the sham group than in the ischemia 30-minute group (P = 0.041) but were not significantly different between the ischemia 45-minute group and ischemia 30-minute group (P = 0.055). VCAM-1 expression in ischemia 45-minute group was significantly higher than in ischemia 30-minute group and sham group(P = 0.003, P < 0.001), and it was positively correlated with the hepatic replacement area (r = 0.491, P = 0.045).
  
  
  CONCLUSIONS
  Hepatic I/R promotes liver hematogenic metastasis of hepatocellular carcinoma in mice and at least in part, through the induction of VCAM-1 expression.
authors.affiliation: Department of Hepatobiliary Surgery,Qilu Hospital of Shandong University,Jinan 250012,China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Hepatobiliary Surgery,Qilu Hospital of Shandong University,Jinan 250012,China.
authors.forename: Shang-lei
authors.initials: SL
authors.lastname: Ning
authors.affiliation: Department of Hepatobiliary Surgery,Qilu Hospital of Shandong University,Jinan 250012,China.
authors.forename: Yu-xin
authors.initials: YX
authors.lastname: Chen
authors.affiliation: Department of Hepatobiliary Surgery,Qilu Hospital of Shandong University,Jinan 250012,China.
authors.forename: Ke-sen
authors.initials: KS
authors.lastname: Xu
authors.affiliation: Department of Hepatobiliary Surgery,Qilu Hospital of Shandong University,Jinan 250012,China.
authors.forename: Nan-hai
authors.initials: NH
authors.lastname: Shou
mesh_terms: D000818:Animals; D008099:Liver / Q000098:blood supply; D008113:Liver Neoplasms / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis; D015427:Reperfusion Injury / Q000150:complications*; D019010:Vascular Cell Adhesion Molecule-1 / Q000502:physiology*
chemical_list: D019010:Vascular Cell Adhesion Molecule-1