title: Metabolomic strategy for the detection of metabolic effects of spermine supplementation in weaned rats.
abstract: The purpose of this study is to examine the effects of spermine supplementation on weaned rat metabolism. A metabolomic strategy employing high-resolution (1)H NMR spectroscopy and multivariate data analysis was used to investigate rat biological responses to spermine ingestion. Rats received intragastric administration of either 0.2 or 0.4 μmol/g body weight of spermine or saline for 3 days. Plasma samples taken 48 h after the last spermine ingestion were analyzed. Spermine supplementation significantly increased the plasma levels of 1-methylhistidine, 3-hydroxybutyrate, alanine, glutamate, glycerolphosphocholine, phosphorylcholine, myo-inositol, phenylalanine, lysine, glutamine, trimethylanine, tyrosine, valine, formate, glucose, and lipids. These results suggest that spermine ingestion can alter common systemic metabolic processes, including cell membrane metabolism, lipid metabolism, glucose-alanine cycle metabolism, amino acid metabolism, and gut microbiota metabolism. This study also shows the important role of spermine administration in modulating the metabolism of weaned rats.
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University , Chengdu 611130, Sichuan, China.
authors.forename: Guangmang
authors.initials: G
authors.lastname: Liu
authors.forename: Tingting
authors.initials: T
authors.lastname: Fang
authors.forename: Tao
authors.initials: T
authors.lastname: Yan
authors.forename: Gang
authors.initials: G
authors.lastname: Jia
authors.forename: Hua
authors.initials: H
authors.lastname: Zhao
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Huang
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Bai
authors.initials: B
authors.lastname: Xue
mesh_terms: D000409:Alanine / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D019587:Dietary Supplements; D005947:Glucose / Q000378:metabolism; D007422:Intestines / Q000382:microbiology; D050356:Lipid Metabolism / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055432:Metabolomics; D064307:Microbiota / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013096:Spermine / Q000008:administration & dosage*; D014886:Weaning
chemical_list: D000596:Amino Acids; D013096:Spermine; D005947:Glucose; D000409:Alanine
keywords: metabolism; metabolomics; plasma; spermine; weaning stress