title: Genomic and peptidomic analyses of the neuropeptides from the emerging pest, Drosophila suzukii.
abstract: Drosophila suzukii is a highly polyphagous invasive pest which has been recently introduced into Europe and North America, where it is causing severe economic losses through larval infestations of stone and berry fruits. The peptidome of the selected nervous tissues of adult D. suzukii was investigated as a first step in identifying potential targets for the development of novel insecticides. Through in silico analyses of the D. suzukii genome databases 28 neuropeptide families, comprising more than 70 predicted peptides were identified. Using a combination of liquid chromatography and mass spectrometry of tissue extracts, 33 predicted peptides, representing 15 different peptide families were identified by their molecular masses and a total of 17 peptide sequences were confirmed by ion fragmentation. A comparison between the peptides and precursors of D. suzukii and D. melanogaster shows they are highly conserved, with differences only identified in the amino acid sequences of the peptides encoded in the FMRFamide, hugin and ecydysis triggering hormone precursors. All other peptides predicted and identified from D. suzukii appear to be identical to those previously characterized from D. melanogaster. Adipokinetic hormone was only identified in the corpus cardiacum, other peptides present included short neuropeptide F, a pyrokinin and myosuppressin, the latter of which was the only peptide identified from the crop nerve bundle. Peptides present in extracts of the brain and/or thoracico-abdominal ganglion included allatostatins, cardioacceleratory peptide 2b, corazonin, extended FMRFamides, pyrokinins, myoinihibitory peptides, neuropeptide-like precursor 1, SIFamide, short neuropeptide F, kinin, sulfakinins and tachykinin related peptides.
authors.affiliation: The Food and Environmental Research Agency, Sand Hutton, York YO41 1LZ, UK. Electronic address: neil.audsley@fera.gsi.gov.uk.
authors.forename: Neil
authors.initials: N
authors.lastname: Audsley
authors.affiliation: The Food and Environmental Research Agency, Sand Hutton, York YO41 1LZ, UK.
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Down
authors.affiliation: Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.
authors.forename: R Elwyn
authors.initials: RE
authors.lastname: Isaac
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004330:Drosophila / Q000737:chemistry* / Q000378:metabolism; D029721:Drosophila Proteins / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D023281:Genomics; D058865:Introduced Species; D008297:Male; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry* / Q000378:metabolism; D009928:Organ Specificity; D020543:Proteome / Q000737:chemistry* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D029721:Drosophila Proteins; D009479:Neuropeptides; D020543:Proteome
keywords: Invasive pest; MALDI-TOF mass spectrometry; Neurohormone; Neuropeptide; Spotted winged Drosophila