title: Two jacalin-related lectins from seeds of the African breadfruit (Treculia africana L.).
abstract: Two jacalin-related lectins (JRLs) were purified by mannose-agarose and melibiose-agarose from seeds of Treculia africana. One is galactose-recognizing JRL (gJRL), named T. africana agglutinin-G (TAA-G), and another one is mannose-recognizing JRL (mJRL), TAA-M. The yields of the two lectins from the seed flour were approximately 7.0 mg/g for gJRL and 7.2 mg/g for mJRL. The primary structure of TAA-G was determined by protein sequencing of lysyl endopeptic peptides and chymotryptic peptides. The sequence identity of TAA-G to other gJRLs was around 70%. Two-residue insertion was found around the sugar-binding sites, compared with the sequences of other gJRLs. Crystallographic studies on other gJRLs have shown that the primary sugar-binding site of gJRLs can accommodate Gal, GalNAc, and GalNAc residue of T-antigen (Galβ1-3GalNAcα-). However, hemagglutination inhibition and glycan array showed that TAA-G did not recognize GalNAc itself and T-antigen. TAA-G preferred melibiose and core 3 O-glycan.
authors.affiliation: a Biochemical Science and Technology, Faculty of Agriculture , Kagoshima University , Kagoshima , Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Shimokawa
authors.forename: Shadrack Makuta
authors.initials: SM
authors.lastname: Nsimba-Lubaki
authors.forename: Namiko
authors.initials: N
authors.lastname: Hayashi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Minami
authors.forename: Fumio
authors.initials: F
authors.lastname: Yagi
authors.forename: Keiko
authors.initials: K
authors.lastname: Hiemori
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Tateno
authors.forename: Jun
authors.initials: J
authors.lastname: Hirabayashi
mesh_terms: D000595:Amino Acid Sequence; D031622:Artocarpus / Q000737:chemistry*; D001665:Binding Sites; D002240:Carbohydrate Sequence; D005690:Galactose / Q000737:chemistry / Q000378:metabolism; D008358:Mannose / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D037121:Plant Lectins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011485:Protein Binding; D059748:Proteolysis; D012639:Seeds / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
chemical_list: D010455:Peptides; D010936:Plant Extracts; D037121:Plant Lectins; C043696:jacalin; D008358:Mannose; D005690:Galactose
keywords: Moraceae; Treculia africana; core 3 O-glycan; galactose-binding; jacalin-related lectin