title: The use of the ex vivo Chandler Loop Apparatus to assess the biocompatibility of modified polymeric blood conduits.
abstract: The foreign body reaction occurs when a synthetic surface is introduced to the body. It is characterized by adsorption of blood proteins and the subsequent attachment and activation of platelets, monocyte/macrophage adhesion, and inflammatory cell signaling events, leading to post-procedural complications. The Chandler Loop Apparatus is an experimental system that allows researchers to study the molecular and cellular interactions that occur when large volumes of blood are perfused over polymeric conduits. To that end, this apparatus has been used as an ex vivo model allowing the assessment of the anti-inflammatory properties of various polymer surface modifications. Our laboratory has shown that blood conduits, covalently modified via photoactivation chemistry with recombinant CD47, can confer biocompatibility to polymeric surfaces. Appending CD47 to polymeric surfaces could be an effective means to promote the efficacy of polymeric blood conduits. Herein is the methodology detailing the photoactivation chemistry used to append recombinant CD47 to clinically relevant polymeric blood conduits and the use of the Chandler Loop as an ex vivo experimental model to examine blood interactions with the CD47 modified and control conduits.
authors.affiliation: Division of Cardiology, Department of Pediatrics, The Children's Hospital of Philadelphia; University of Pennsylvania Perelman School of Medicine.
authors.forename: Joshua B
authors.initials: JB
authors.lastname: Slee
authors.affiliation: Division of Cardiology, Department of Pediatrics, The Children's Hospital of Philadelphia; University of Pennsylvania Perelman School of Medicine.
authors.forename: Ivan S
authors.initials: IS
authors.lastname: Alferiev
authors.affiliation: Division of Cardiology, Department of Pediatrics, The Children's Hospital of Philadelphia; University of Pennsylvania Perelman School of Medicine.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Levy
authors.affiliation: Division of Cardiology, Department of Pediatrics, The Children's Hospital of Philadelphia; University of Pennsylvania Perelman School of Medicine; Stachelek@email.chop.edu.
authors.forename: Stanley J
authors.initials: SJ
authors.lastname: Stachelek
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D001790:Blood Physiological Phenomena; D001792:Blood Platelets / Q000737:chemistry / Q000166:cytology; D051928:CD47 Antigen; D005112:Extracorporeal Circulation / Q000295:instrumentation*; D005549:Foreign-Body Reaction / Q000097:blood* / Q000139:chemically induced*; D006801:Humans; D008422:Materials Testing / Q000295:instrumentation* / Q000379:methods; D011108:Polymers / Q000737:chemistry*; D011994:Recombinant Proteins
chemical_list: D001672:Biocompatible Materials; D051928:CD47 Antigen; C497894:CD47 protein, human; D011108:Polymers; D011994:Recombinant Proteins