title: Involvement of aquaporins in a mouse model of rotavirus diarrhea.
abstract: Rotavirus diarrhea is a major worldwide cause of infantile gastroenteritis; however, the mechanism responsible for intestinal fluid loss remains unclear. Water transfer across the intestinal epithelial membrane seems to occur because of aquaporins (AQPs). Accumulating evidence indicates that alterations in AQPs may play an important role in pathogenesis. Here, we focus on changes in AQPs in a mouse model of rotavirus diarrhea. In the present study, 32 of 35 mice developed diarrhea and mild dehydration within 24 hours after infection with rotavirus strain SA11. Intestinal epithelial cells demonstrated cytoplasmic vacuolation, malaligned villi, and atrophy. AQP1 expression was significantly attenuated in the ileum and colon in comparison with controls; likewise, AQP4 and -8 protein expression were significantly decreased in the colon of rotavirus diarrhea-infected mice. In contrast, AQP3 protein expression was significantly increased in the colon of rotavirus-infected mice in comparison with controls. These results indicate that rotavirus diarrhea is associated with the downregulation of AQP1, -4, and -8 expression. Therefore, AQPs play an important role in rotavirus diarrhea.
authors.affiliation: Department of Gastroenterology, Guangzhou Women and Children's Medical Center, Guangzhou, 510623, China.
authors.forename: Meiwan
authors.initials: M
authors.lastname: Cao
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Ou
authors.forename: Dingyou
authors.initials: D
authors.lastname: Li
authors.forename: Lanlan
authors.initials: L
authors.lastname: Geng
authors.forename: Peiyu
authors.initials: P
authors.lastname: Chen
authors.forename: Huan
authors.initials: H
authors.lastname: Chen
authors.forename: Sitang
authors.initials: S
authors.lastname: Gong
mesh_terms: D000818:Animals; D051398:Aquaporin 1 / Q000235:genetics / Q000378:metabolism*; D051400:Aquaporin 3 / Q000235:genetics / Q000378:metabolism; D051401:Aquaporin 4 / Q000235:genetics / Q000378:metabolism*; D020346:Aquaporins / Q000235:genetics / Q000378:metabolism*; D003106:Colon / Q000378:metabolism; D003967:Diarrhea / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D004195:Disease Models, Animal; D015536:Down-Regulation; D006801:Humans; D051379:Mice; D012401:Rotavirus / Q000502:physiology*; D012400:Rotavirus Infections / Q000235:genetics / Q000378:metabolism*
chemical_list: C496021:Aqp1 protein, mouse; C496028:Aqp3 protein, mouse; C496031:Aqp4 protein, mouse; D051401:Aquaporin 4; D020346:Aquaporins; C108330:aquaporin 8; D051398:Aquaporin 1; D051400:Aquaporin 3