title: Preliminary enrichment and separation of chlorogenic acid from Helianthus tuberosus L. leaves extract by macroporous resins.
abstract: In the present study, a simple and efficient method for the preparative separation of 3-CQA from the extract of Helianthus tuberosus leaves with macroporous resins was studied. ADS-21 showed much higher adsorption capacity and better adsorption/desorption properties for 3-CQA among the tested resins. The adsorption of 3-CQA on ADS-21 resin at 25°C was fitted best to the Langmuir isotherm model and pseudo-second-order kinetic model. Dynamic adsorption/desorption experiments were carried out in a glass column packed with ADS-21 to optimise the separation process of 3-CQA from H. tuberosus leaves extract. After one treatment with ADS-21, the content of 3-CQA in the product was increased 5.42-fold, from 12.0% to 65.2%, with a recovery yield of 89.4%. The results demonstrated that the method was suitable for large-scale separation and manufacture of 3-CQA from H. tuberosus leaves.
authors.affiliation: Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China; College of Chemistry, Liaoning University, Shenyang 110036, PR China.
authors.forename: Peng-Cheng
authors.initials: PC
authors.lastname: Sun
authors.affiliation: Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China. Electronic address: yliu@yic.ac.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
authors.forename: Yue-Tao
authors.initials: YT
authors.lastname: Yi
authors.affiliation: Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
authors.forename: Hong-Juan
authors.initials: HJ
authors.lastname: Li
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Fan
authors.affiliation: Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China. Electronic address: chxia@yic.ac.cn.
authors.forename: Chuan-Hai
authors.initials: CH
authors.lastname: Xia
mesh_terms: D000327:Adsorption; D002726:Chlorogenic Acid / Q000032:analysis / Q000302:isolation & purification*; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D006368:Helianthus / Q000737:chemistry*; D007700:Kinetics; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D012117:Resins, Synthetic; D002726:Chlorogenic Acid
keywords: Chlorogenic acid; Helianthus tuberosus L.; Macroporous resin; Separation