title: HLA genotyping in pediatric celiac disease patients.
abstract: Celiac disease (CD) is a chronic inflammatory disease in the small intestine triggered by gluten uptake that occurs in genetically susceptible individuals. HLA-DQ2 protein encoded by HLA-DQA1*05 and DQB1*02 alleles is found in 90-95% of CD patients. All of the remaining patients carry HLA-DQ8 protein encoded by HLA-DQA1*03 and DQB1*03:02 alleles. Specific HLA-DQ genotypes define different risk for CD incidence. Presence of susceptible HLA-DQ genotypes does not predict certain disease development, but their absence makes CD very unlikely, close to 100%. Here we presented for the first time the distribution of HLA-DQ genotypes in the group of pediatric celiac patients from the University Children's Hospital, Belgrade, Serbia and estimated risk for CD development that these genotypes confer. Seventy three celiac disease patients and 62 healthy individuals underwent genotyping for DQA1, DQB alleles and DRB1 allele. 94.5% of patients carried alleles that encode DQ2 protein variant and 2.7% carried alleles that encode DQ8 protein variant. Two patients carried single DQB1*02 allele. No patients were negative for all the alleles predisposing to CD. The highest HLA-DQ genotype risk for CD development was found in group of patients homozygous for DQ2.5 haplotype, followed by the group of heterozygous carriers of DQ2.5 haplotype in combination with DQB1*02 allele within the other haplotype. The lowest risk was observed in carriers of a single copy of DQB1*02 or DQA1*05 allele or other non-predisposing alleles. HLA genotyping, more informative than serological testing commonly used, proved to be a useful diagnostic tool for excluding CD development.
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade. bi.stankovic@gmail.com.
authors.forename: Biljana
authors.initials: B
authors.lastname: Stanković
authors.forename: Nedeljko
authors.initials: N
authors.lastname: Radlović
authors.forename: Zoran
authors.initials: Z
authors.lastname: Leković
authors.forename: Dragana
authors.initials: D
authors.lastname: Ristić
authors.forename: Vladimir
authors.initials: V
authors.lastname: Radlović
authors.forename: Gordana
authors.initials: G
authors.lastname: Nikčević
authors.forename: Nikola
authors.initials: N
authors.lastname: Kotur
authors.forename: Ksenija
authors.initials: K
authors.lastname: Vučićević
authors.forename: Tatjana
authors.initials: T
authors.lastname: Kostić
authors.forename: Sonja
authors.initials: S
authors.lastname: Pavlović
authors.forename: Branka
authors.initials: B
authors.lastname: Zukic
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D002446:Celiac Disease / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D059848:HLA-DQ alpha-Chains / Q000235:genetics; D006239:Haplotypes; D006720:Homozygote; D006801:Humans; D007223:Infant; D007249:Inflammation; D008297:Male; D055815:Young Adult
chemical_list: D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C053186:HLA-DQ2 antigen; C109756:HLA-DQ8 antigen; C069050:HLA-DQA1 antigen