title: Biochemical and histological effects of gibberellic acid on Locusta migratoria migratoria fifth instar larvae.
abstract: Experiments were conducted to assess the effect of gibberellic acid (GA3), a plant growth regulator, on Locusta migratoria migratoria fifth instar larvae. Newly emerged larvae were exposed to various concentrations of GA3 administered by topical application or by forced ingestion. Results showed that treated insects exhibited toxic symptoms with a dose-dependent mortality. GA3 toxicity was also demonstrated by perturbation of the moult processes. In fact, we noted that treated insects present exuviations difficulties due to the impossibility to reject the old integuments causing mortality in the 5th instar larvae. Histological study of proventriculus revealed alterations in the epithelial cells and absence of apolysis phenomenon. Data also showed that GA3 induced significant quantitative variation of haemolymph metabolites. These changes result in a significant decrease in the total concentration of proteins and carbohydrates and an increase in the total concentration of haemolymph lipids.
authors.affiliation: Département des Sciences Biologiques et de la Protection des Végétaux, Institut Supérieur Agronomique de Chott-Mariem, 4042, Université de Sousse , Tunisia. Electronic address: kemais_a@yahoo.fr.
authors.forename: Khemais
authors.initials: K
authors.lastname: Abdellaoui
authors.affiliation: Département des Sciences Biologiques et de la Protection des Végétaux, Institut Supérieur Agronomique de Chott-Mariem, 4042, Université de Sousse , Tunisia.
authors.forename: Monia
authors.initials: M
authors.lastname: Ben Halima-Kamel
authors.affiliation: Département de Biologie, Faculté des Sciences, Université M'hamed Bougara, Avenue de l'indépendance, 35000 Boumerdes, Algeria.
authors.forename: Fatma
authors.initials: F
authors.lastname: Acheuk
authors.affiliation: Laboratoire de Biologie Animale Appliquée, Département de Biologie, Université Badji Mokhtar, 23000 Annaba, Algeria.
authors.forename: Noureddine
authors.initials: N
authors.lastname: Soltani
authors.affiliation: Laboratoire de Biologie Animale Appliquée, Département de Biologie, Université Badji Mokhtar, 23000 Annaba, Algeria.
authors.forename: Nadia
authors.initials: N
authors.lastname: Aribi
authors.affiliation: Département des Sciences Biologiques et de la Protection des Végétaux, Institut Supérieur Agronomique de Chott-Mariem, 4042, Université de Sousse , Tunisia.
authors.forename: Mohamed HabibBen
authors.initials: MH
authors.lastname: Hamouda
mesh_terms: D000818:Animals; D004064:Digestive System / Q000187:drug effects / Q000473:pathology; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D005216:Fat Body / Q000187:drug effects / Q000473:pathology; D005875:Gibberellins / Q000494:pharmacology*; D006458:Hemolymph / Q000378:metabolism; D007306:Insecticides / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D048188:Locusta migratoria / Q000187:drug effects* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology*
chemical_list: D005875:Gibberellins; D007306:Insecticides; D010937:Plant Growth Regulators; C007842:gibberellic acid
keywords: Gibberellic acid; Haemolymph metabolites; Histology; L. migratoria; Toxicity