title: O-Succinyl-L-homoserine-based C4-chemical production: succinic acid, homoserine lactone, γ-butyrolactone, γ-butyrolactone derivatives, and 1,4-butanediol.
abstract: There has been a significant global interest to produce bulk chemicals from renewable resources using engineered microorganisms. Large research programs have been launched by academia and industry towards this goal. Particularly, C4 chemicals such as succinic acid (SA) and 1,4-butanediol have been leading the path towards the commercialization of biobased technology with the effort of replacing chemical production. Here we present O-Succinyl-L-homoserine (SH) as a new, potentially important platform biochemical and demonstrate its central role as an intermediate in the production of SA, homoserine lactone (HSL), γ-butyrolactone (GBL) and its derivatives, and 1,4-butanediol (BDO). This technology encompasses (1) the genetic manipulation of Escherichia coli to produce SH with high productivity, (2) hydrolysis into SA and homoserine (HS) or homoserine lactone hydrochloride, and (3) chemical conversion of either HS or homoserine lactone HCL (HSL·HCl) into drop-in chemicals in polymer industry. This production strategy with environmental benefits is discussed in the perspective of targeting of fermented product and a process direction compared to petroleum-based chemical conversion, which may reduce the overall manufacturing cost.
authors.affiliation: Research Institute of Biotechnology, CJ CheilJedang, Seoul, 157-724, Korea.
authors.forename: Kuk-Ki
authors.initials: KK
authors.lastname: Hong
authors.forename: Jeong Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Jong Hyun
authors.initials: JH
authors.lastname: Yoon
authors.forename: Hye-Min
authors.initials: HM
authors.lastname: Park
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Choi
authors.forename: Gyu Hyeon
authors.initials: GH
authors.lastname: Song
authors.forename: Jea Chun
authors.initials: JC
authors.lastname: Lee
authors.forename: Young-Lyeol
authors.initials: YL
authors.lastname: Yang
authors.forename: Hyun Kwan
authors.initials: HK
authors.lastname: Shin
authors.forename: Ju Nam
authors.initials: JN
authors.lastname: Kim
authors.forename: Kyung Ho
authors.initials: KH
authors.lastname: Cho
authors.forename: Jung Ho
authors.initials: JH
authors.lastname: Lee
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000096:biosynthesis; D019149:Bioreactors; D002072:Butylene Glycols / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D006714:Homoserine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D006868:Hydrolysis; D012995:Solubility; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D002072:Butylene Glycols; C088386:homoserine lactone; C014196:O-succinylhomoserine; D006714:Homoserine; C039681:1,4-butanediol; D019802:Succinic Acid; D015107:4-Butyrolactone