title: Effect of Lactobacillus species on Streptococcus mutans biofilm formation.
abstract: Streptococcus mutans is the primary pathogen responsible for initiating dental caries and decay. The presence of sucrose, stimulates S. mutans to produce insoluble glucans to form oral biofilm also known as dental plaque to initiate caries lesion. The GtfB and LuxS genes of S. mutans are responsible for formation and maturation of biofilm. Lactobacillus species as probiotic can reduces the count of S. mutans. In this study effect of different Lactobacillus species against the formation of S. mutans biofilm was observed. Growing biofilm in the presence of sucrose was detected using 96 well microtiter plate crystal violet assay and biofilm formation by S. mutans in the presence of Lactobacillus was detected. Gene expression of biofilm forming genes (GtfB and LuxS) was quantified through Real-time PCR. All strains of Lactobacillus potently reduced the formation of S. mutans biofilm whereas Lactobacillus acidophilus reduced the genetic expression by 60-80%. Therefore, probiotic Lactobacillus species can be used as an alternative instead of antibiotics to decrease the chance of dental caries by reducing the count of S. mutans and their gene expression to maintain good oral health.
authors.affiliation: Dr. Panjwani Center for Molecular Medicine & Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan.
authors.forename: Ayaz
authors.initials: A
authors.lastname: Ahmed
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, Liaoning, PR China.
authors.forename: Wu
authors.initials: W
authors.lastname: Dachang
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, Liaoning, PR China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Lei
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, Liaoning, PR China.
authors.forename: Liu
authors.initials: L
authors.lastname: Jianjun
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, Liaoning, PR China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Juanjuan
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, Liaoning, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development*; D013437:Carbon-Sulfur Lyases / Q000235:genetics / Q000378:metabolism; D003731:Dental Caries / Q000382:microbiology / Q000517:prevention & control*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007778:Lactobacillus / Q000145:classification / Q000502:physiology*; D019936:Probiotics; D013295:Streptococcus mutans / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D013395:Sucrose; D016695:Glycosyltransferases; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria