title: Introduction to RNA-Seq and its applications to drug discovery and development.
abstract: Preclinical Research RNA sequencing (RNA-Seq) is a novel high-throughput technology for comprehensive transcriptome study. It can measure the expression levels of thousands of genes simultaneously and provide insight into functional pathways and regulations in biological processes. In addition, RNA-Seq can provide copious information on alternative splicing, allele-specific expression, unannotated exons, and novel transcripts (gene or noncoding RNAs). This technology has revolutionized the way biologists examine transcriptomes and has been successfully applied in drug discovery and development, being able to identify drug-related genes, microRNAs, and fusion proteins. In this overview, we will review this technology including data analysis, and its recent applications in drug discovery and development.
authors.affiliation: Department of Pharmacodynamics, College of Pharmacy, University of Florida, Gainesville, FL, 32610-0484, USA.
authors.forename: Zainab
authors.initials: Z
authors.lastname: Khatoon
authors.forename: Bryan
authors.initials: B
authors.lastname: Figler
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Cheng
mesh_terms: D055808:Drug Discovery; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D017423:Sequence Analysis, RNA; D059467:Transcriptome
keywords: RNA-Seq; drug resistance; fusion protein; miRNA; microarray; next-generation sequencing