title: Ectopic expression of FaesAP3, a Fagopyrum esculentum (Polygonaceae) AP3 orthologous gene rescues stamen development in an Arabidopsis ap3 mutant.
abstract: Arabidopsis thaliana APETALA3 (AP3) and Antirrhinum majus DEFICIENS (DEF) MADS box genes are required to specify petal and stamen identity. AP3 and DEF are members of the euAP3 lineage, which arose by gene duplication coincident with radiation of the core eudicots. In order to investigate the molecular mechanisms underlying organ development in early diverging clades of core eudicots, we isolated and identified an AP3 homolog, FaesAP3, from Fagopyrum esculentum (buckwheat, Polygonaceae), a multi-food-use pseudocereal with healing benefits. Protein sequence alignment and phylogenetic analyses revealed that FaesAP3 grouped into the euAP3 lineage. Expression analysis showed that FaesAP3 was transcribed only in developing stamens, and differed from AP3 and DEF, which expressed in developing petals and stamens. Moreover, ectopic expression of FaesAP3 rescued stamen development without complementation of petal development in an Arabidopsis ap3 mutant. Our results suggest that FaesAP3 is involved in the development of stamens in buckwheat. These results also suggest that FaesAP3 holds some potential for biotechnical engineering to create a male sterile line of F. esculentum.
authors.affiliation: Institute of Crop Genetics and Breeding, Yangtze University, Jingzhou City, Hubei 434025, PR China. Electronic address: Fangzhengwu88@163.com.
authors.forename: Zheng-wu
authors.initials: ZW
authors.lastname: Fang
authors.affiliation: College of Horticulture and Gardening, Yangtze University, Jingzhou City, Hubei 434025, PR China. Electronic address: qirui0502@126.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Qi
authors.affiliation: Institute of Crop Genetics and Breeding, Yangtze University, Jingzhou City, Hubei 434025, PR China. Electronic address: lixiaofang@163.net.
authors.forename: Xiao-fang
authors.initials: XF
authors.lastname: Li
authors.affiliation: Institute of Crop Genetics and Breeding, Yangtze University, Jingzhou City, Hubei 434025, PR China; College of Horticulture and Gardening, Yangtze University, Jingzhou City, Hubei 434025, PR China. Electronic address: zxliu77@yahoo.com.
authors.forename: Zhi-xiong
authors.initials: ZX
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D019612:Fagopyrum / Q000235:genetics*; D035264:Flowers / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018014:Gene Transfer Techniques; D026161:MADS Domain Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D030821:Plants, Genetically Modified; D017385:Sequence Homology
chemical_list: C101342:APETALA 3 protein, Arabidopsis; D029681:Arabidopsis Proteins; D026161:MADS Domain Proteins
keywords: APETALA3; B-function; Buckwheat; Floral development