title: Effect of uraemic status on immunofluorescence and enzyme-linked immunosorbent assays for detection of human herpesvirus type 8 antibodies.
abstract: Our previous study showed that human herpesvirus type 8 (HHV-8) seroprevalence was significantly higher in patients with end-stage renal disease (ESRD) immediately after haemodialysis than in healthy controls based on an immunofluorescence assay (IFA) and an enzyme-linked immunosorbent assay (ELISA). However, other studies indicated that ESRD patients and healthy controls had similar HHV-8 seroprevalence. This study aimed to investigate whether this discrepancy is due to the effect of uraemic status.Plasma samples from 162 ESRD patients, taken immediately before and after haemodialysis, and 162 age and sex matched healthy controls were analysed for HHV-8 antibodies using both IFA and ELISA.HHV-8 seropositivities based on IFA and ELISA, both before and after haemodialysis, were significantly greater in ESRD patients than in healthy controls (p < 0.008 for all comparisons). The seropositivities and antibody titres of ESRD patients obtained with IFA were similar before and after haemodialysis. Seropositivities based on ELISA were identical before and after haemodialysis. The seropositivities obtained with the IFA markedly exceeded those with ELISA in each group of subjects (p < 0.0001 for all comparisons).Uraemic status did not significantly affect the IFA or ELISA results for HHV-8 antibodies.
authors.affiliation: 1Department of Nephrology, Buddhist Dalin Tzu Chi Hospital, Chiayi 2Institute of Medicine, Chung Shan Medical University, Taichung 3Departments of Family Medicine 4Clinical Pathology 5Anatomic Pathology, Buddhist Dalin Tzu Chi Hospital, Chiayi 6Institute of Medical Sciences, Tzu Chi University, Hualien 7Departments of Laboratory Medicine and Pathology, School of Medicine, Tzu Chi University, Hualien, Taiwan.
authors.forename: Jen-Pi
authors.initials: JP
authors.lastname: Tsai
authors.forename: Ming-Nan
authors.initials: MN
authors.lastname: Lin
authors.forename: Cheng-Chuan
authors.initials: CC
authors.lastname: Su
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods; D006566:Herpesviridae Infections / Q000150:complications / Q000175:diagnosis* / Q000276:immunology; D019288:Herpesvirus 8, Human / Q000276:immunology*; D006801:Humans; D016867:Immunocompromised Host; D007676:Kidney Failure, Chronic / Q000150:complications* / Q000276:immunology / Q000628:therapy / Q000821:virology; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis; D016036:Seroepidemiologic Studies; D014511:Uremia / Q000150:complications* / Q000276:immunology / Q000628:therapy / Q000821:virology; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral