title: An immunohistochemical approach for monitoring effects of exercise on tumor stromal cells in old mice.
abstract: Epidemiological evidence supports a protective effect of physical activity for breast cancer in older women, but the mechanisms are not well understood. We used 18-month-old BALB/c mice injected in the mammary fat pad with syngeneic 4T1 tumor cells as a model of invasive breast cancer. During the tumor progression phase, there was a significant decrease in labeling for F4/80, a marker for mouse macrophages, and CD34, a marker for vascular endothelial cells, in primary tumors from mice that ran higher average distances compared to mice that ran lower average distances (p≤0.05). These observations suggest that immunohistochemistry can be used to monitor stromal cell populations in tumors from old mice under exercise conditions.
authors.affiliation: Department of Comparative Medicine, University of Washington, Seattle WA, USA.
authors.forename: Christina
authors.initials: C
authors.lastname: Pettan-Brewer
authors.affiliation: Department of Comparative Medicine, University of Washington, Seattle WA, USA ; Interdisciplinary Program in Nutritional Sciences, University of Washington, Seattle WA, USA.
authors.forename: Jorming
authors.initials: J
authors.lastname: Goh
authors.affiliation: Department of Comparative Medicine, University of Washington, Seattle WA, USA.
authors.forename: Warren C
authors.initials: WC
authors.lastname: Ladiges
keywords: aging; breast cancer; exercise; immunohistochemistry; tumor microenvironment; tumor stromal cells