title: Bone defect repair in mice by mesenchymal stem cells.
abstract: Adult bone marrow niche contains rare primitive but highly functional multipotent progenitors (e.g., mesenchymal stem cells; MSCs) capable of differentiating into specific mesenchymal tissues like bone, cartilage, muscle, fat tissues, ligament, dermis, bone marrow stroma, tendon, and other connective tissues. Upon in vivo transplantation, MSCs also secrete a wide range of growth factors, immunomodulatory cytokines, and important bioactive macromolecules including cell-derived exosomes to structure regenerative microenvironments. This protocol describes a mouse model to study bone formation/regeneration from adult mesenchymal stem cells.
authors.affiliation: Center for Stem Cell Research, Christian Medical College, Bagayam, Vellore, Tamilnadu, 632002, India, skumar@cmcvellore.ac.in.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D016267:External Fixators; D005260:Female; D017102:Fracture Healing; D050723:Fractures, Bone / Q000000981:diagnostic imaging / Q000534:rehabilitation / Q000628:therapy; D006085:Graft Survival; D007150:Immunohistochemistry; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D010012:Osteogenesis; D011859:Radiography