title: [Identification and denitrification characteristics of a psychrotolerant facultative basophilic aerobic denitrifier].
abstract: An aerobic denitrifier was isolated from the activated sludge of landfill leachate through traditional microbiological methods. Based on its morphological feature, physiological and biochemical properties, and 16S rDNA sequence analysis, this strain was identified as Pseudomonas sp., named as GL19 with an accession number of KC710974 in GenBank. Its aerobic denitrification characteristics and nitrification function were studied to show that the factors including carbon source, C/N, pH and cultivation temperature were important for denitrification. The optimized condition for aerobic denitrification was as follows: sodium citrate as the carbon resource, C/N no less than 15, pH of 6-10, DO of 4.8-7.7 mg x L(-1), culture temperature of 15-34 degrees C and the initial nitrate nitrogen of 140 mg x L(-1). Combining these conditions, the removal rate of nitrate nitrogen and average removal rate of TN reached 100% and 96.5%, respectively, without the accumulation of nitrite nitrogen. The strain had the capability to utilize nitrite nitrogen or ammonia nitrogen to achieve high nitrogen removal efficiency: the nitrite nitrogen removal rate reached 100% in 20 hours with an initial nitrite nitrogen of 140 mg x L(-1); the ammonia nitrogen was efficiently removed from 280 mg x L(-1) to 3.11 mg x L(-1) in 28 hours with the removal rate of up to 98.9%. These results suggested that strain GL19 with the function of cold resistance and highly effective aerobic denitrification could achieve simultaneous nitrification and denitrification. Hence, GL19 could have high potential in practical wastewater treatment in winter of south area.
authors.forename: Zhao-Yang
authors.initials: ZY
authors.lastname: Wang
authors.forename: Guo-Yao
authors.initials: GY
authors.lastname: Chen
authors.forename: Ke
authors.initials: K
authors.lastname: Jiang
authors.forename: Pei-Ya
authors.initials: PY
authors.lastname: Xu
mesh_terms: D002244:Carbon / Q000737:chemistry; D058440:Denitrification; D009566:Nitrates / Q000737:chemistry; D058465:Nitrification; D009573:Nitrites / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry; D011549:Pseudomonas / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D062065:Wastewater / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000737:chemistry
chemical_list: D002244:Carbon; D009566:Nitrates; D009573:Nitrites; D009584:Nitrogen; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D062065:Wastewater; D014874:Water Pollutants, Chemical