title: In vitro-in vivo evaluation of lipid based formulations of the CETP inhibitors CP-529,414 (torcetrapib) and CP-532,623.
abstract: The present study investigated the use of lipid based drug delivery systems to enhance the oral bioavailability of the CETP inhibitors CP-532,623 and torcetrapib. A series of self-emulsifying lipid based drug delivery systems (SEDDS) were assembled and examined using an in vitro lipid digestion model to evaluate patterns of drug precipitation under simulated intestinal conditions. Drug exposure after oral administration of the same formulations was subsequently assessed in beagle dogs. CP-532,623 was maintained in a solubilised state during dispersion of most formulations in simulated intestinal fluid, however, solubilisation capacity was reduced to various degrees upon in vitro digestion. Administration of SEDDS formulations to beagle dogs resulted in moderate differences in plasma AUC when compared to the differences in solubilisation observed in vitro. Similar trends were observed for torcetrapib. In all cases, however, in vivo exposure of CP-532,623 was greatly enhanced by administration in lipid based drug delivery systems when compared to a powder formulation. Some correlation between in vitro solubilisation and in vivo drug exposure (AUC) was evident; however, this was not linear. The data suggest that for highly lipophilic drugs such as CP-532,623 in vitro digestion data may be a conservative in vitro indicator of utility and that good exposure may be evident even for formulations that result in significant drug precipitation during in vitro digestion.
authors.affiliation: Drug Delivery Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Australia.
authors.forename: Claire L
authors.initials: CL
authors.lastname: McEvoy
authors.affiliation: Drug Delivery Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Australia.
authors.forename: Natalie L
authors.initials: NL
authors.lastname: Trevaskis
authors.affiliation: Department of Veterinary Sciences, The University of Melbourne, Werribee, Australia.
authors.forename: Glenn A
authors.initials: GA
authors.lastname: Edwards
authors.affiliation: Pfizer Global Research and Development, Groton, CT, USA.
authors.forename: Michael E
authors.initials: ME
authors.lastname: Perlman
authors.affiliation: Pfizer Global Research and Development, Groton, CT, USA.
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Ambler
authors.affiliation: Pfizer Global Research and Development, Groton, CT, USA.
authors.forename: Mary C
authors.initials: MC
authors.lastname: Mack
authors.affiliation: Pfizer Global Research and Development, Groton, CT, USA.
authors.forename: Barbara
authors.initials: B
authors.lastname: Brockhurst
authors.affiliation: Drug Delivery Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Australia. Electronic address: chris.porter@monash.edu.
authors.forename: Christopher J H
authors.initials: CJ
authors.lastname: Porter
mesh_terms: D000818:Animals; D002626:Chemistry, Pharmaceutical; D053480:Cholesterol Ester Transfer Proteins / Q000037:antagonists & inhibitors*; D018592:Cross-Over Studies; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D008055:Lipids / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D011804:Quinolines / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: C544076:CP 532,623; D053480:Cholesterol Ester Transfer Proteins; D008055:Lipids; D011804:Quinolines; C483909:torcetrapib
keywords: Bioavailability; Excipient; In vitro digestion; Lipid based drug delivery; Poorly water soluble drug; Solubilisation