title: Influence of HLA-DRB alleles on haemorrhagic fever with renal syndrome in a Chinese Han population in Hubei Province, China.
abstract: Specific human leucocyte antigen (HLA) alleles are considered a genetic risk factor for the progression of haemorrhagic fever with renal syndrome (HFRS) caused by hantaviruses. The aim of this study was to establish whether HLA-DRB alleles are associated with the severity of HFRS caused by different types of hantaviruses in a Chinese Han population from Hubei Province of central China. Twenty-two specific HLA-DRB alleles were analysed by sequence-specific primer-polymerase chain reaction (SSP-PCR) in 100 HFRS patients and 213 healthy volunteers. Associations of HLA-DRB alleles with the severity and clinical parameters of HFRS caused by Hantaan virus (HTNV) or Seoul virus (SEOV) infection were evaluated. Six alleles (HLA-DRB1*0401-0411, HLA-DRB1*1001, HLA-DRB1*1101-1105, HLA-DRB1*1201-1202, HLA-DRB1*1305 and DRB5*0101-0201) demonstrated strong associations with HFRS caused by HTNV and SEOV infections. Further comparison of these HLA-DRB1 allele frequencies between HFRS patients with differing severities and healthy controls demonstrated that the HLA-DRB1*0401-0411, HLA-DRB1*1001 and DRB1*1305 alleles were more frequent in the moderate course of HTNV-infected HFRS. Meanwhile, the DRB1*1101-1105 allele was more frequently observed in the severe course of HTNV-infected HFRS. We also found that the HLA-DRB1*1201-1202 allele frequency was higher in the moderate course of SEOV-infected HFRS, whereas the DRB5*0101-0201 allele may play a protective role in moderate HFRS caused by both HTNV and SEOV infections. These results provide evidence of the influence of HLA-DRB on the severity of HFRS and confirm the effect of HLA-DRB on HFRS during different types of hantavirus infection in a Chinese Han population in Hubei Province, China.
authors.affiliation: State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, 185 Donghu Road, Wuhan, 430071, China.|Department of Microbiology, School of Basic Medical Sciences, Hubei University of Science and Technology, Xianning, 437100, China.
authors.forename: N
authors.initials: N
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, 185 Donghu Road, Wuhan, 430071, China.
authors.forename: F
authors.initials: F
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, 185 Donghu Road, Wuhan, 430071, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, 185 Donghu Road, Wuhan, 430071, China.
authors.forename: N
authors.initials: N
authors.lastname: Li
authors.affiliation: State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, 185 Donghu Road, Wuhan, 430071, China.
authors.forename: H
authors.initials: H
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, 185 Donghu Road, Wuhan, 430071, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, 185 Donghu Road, Wuhan, 430071, China. yangzhanqiu@163.com.
authors.forename: Z
authors.initials: Z
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, 185 Donghu Road, Wuhan, 430071, China. houwei@whu.edu.cn.
authors.forename: W
authors.initials: W
authors.lastname: Hou
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D002681:China; D004198:Disease Susceptibility; D005260:Female; D005787:Gene Frequency; D059806:HLA-DR beta-Chains / Q000235:genetics*; D018063:Hantaan virus / Q000276:immunology / Q000302:isolation & purification; D006480:Hemorrhagic Fever with Renal Syndrome / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D029263:Seoul virus / Q000276:immunology / Q000302:isolation & purification; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D059806:HLA-DR beta-Chains