title: Enumeration of CD4(+)CD25(+)T regulatory cells in Type-II diabetes retinopathy.
abstract: Diabetes mellitus (DM) is a health concern because it leads to complications such as retinopathy. Pakistan has 6.9 million DM affected people that will be doubled by 2025. A study was designed to enumerate CD4(+)CD25(+)Treg cells in Pakistani type 2 diabetes mellitus (T2DM) patients. It was a cross-sectional case-control study that included 212 subjects. The subjects having diabetic retinopathy were labeled as Group-I (30 healthy volunteers without diabetes), Group-II (30 T2DM patients without retinopathy) and Group-III (152 T2DM patients with retinopathy). The percentage of CD4+CD25+ Treg cells was determined by Flowcytometry. Comparison of CD4(+)CD25(+)T cells among different groups was not significant and higher percentage of Treg cells was observed in Group-II (3.07%) compared to Group-III (2.88%). Age, gender and duration of diabetes may contribute while percentage of CD4(+)CD25(+)T cells and Treg cells were not associated with the development of DR in T2DM.
authors.affiliation: Department of Immunology, University of Health Sciences, Lahore, Pakistan.
authors.forename: Nadeem
authors.initials: N
authors.lastname: Afzal
authors.affiliation: Department of Immunology, University of Health Sciences, Lahore, Pakistan.
authors.forename: Khursheed
authors.initials: K
authors.lastname: Javaid
authors.affiliation: Children Hospital & Institute of Child Health, Lahore, Pakistan.
authors.forename: Shakeela
authors.initials: S
authors.lastname: Zaman
authors.affiliation: Amin Hayat Memorial Diabetic Centre, Lahore, Pakistan.
authors.forename: Abu
authors.initials: A
authors.lastname: Zafar
authors.affiliation: Departments of Pathology, University of Health Sciences, Lahore, Pakistan.
authors.forename: Abdul H
authors.initials: AH
authors.lastname: Nagi
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000276:immunology*; D003930:Diabetic Retinopathy / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016015:Logistic Models; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*