title: Antioxidant and cytoprotective properties of partridgeberry polyphenols.
abstract: Partridgeberry (Vaccinium vitis-idaea) is a polyphenol-rich berry of the Ericaceous family, grown in Newfoundland and Labrador province of Canada. The aims of this study were to identify extraction solvents for the maximum recovery of polyphenols, to establish fractionation technique for isolation of major sub-classes of polyphenols, and to evaluate antioxidant and cytoprotective properties of the partridgeberry polyphenol preparations. The acidified 70% acetone was identified as the ideal solvent for the maximum recovery of polyphenols from partridgeberry. Further, aqueous two-phase extraction, column chromatography and UPLC-MS/MS were employed to produce three partridgeberry polyphenol fractions, rich in either, anthocyanins, flavan-3-ols or flavonols. All the three PPF were potent antioxidants and displayed cytoprotective activity through the activation of nuclear factor erythroid 2-related factor 2 pathway, scavenging of reactive oxygen species, and inhibition of cellular death. The current study suggests that partridgeberry has numerous potential health implications in both prevention and amelioration of various diseases involving oxidative stress.
authors.affiliation: Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, NS B2N 5E3, Canada.
authors.forename: Khushwant S
authors.initials: KS
authors.lastname: Bhullar
authors.affiliation: Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, NS B2N 5E3, Canada. Electronic address: vrupasinghe@dal.ca.
authors.forename: H P Vasantha
authors.initials: HP
authors.lastname: Rupasinghe
mesh_terms: D000872:Anthocyanins / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D059625:Liquid-Liquid Extraction; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D059808:Polyphenols / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D029801:Vaccinium vitis-idaea / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D059808:Polyphenols; D020011:Protective Agents; D017382:Reactive Oxygen Species
keywords: (−)-Epicatechin (PubChem CID: 72276); Antioxidant; Caffeic acid (PubChem CID: 689043); Catechin (PubChem CID: 9064); Chlorogenic acid (PubChem CID: 1794427); Cytoprotection; Epigallocatechin (PubChem CID: 72277); Ferulic acid (PubChem CID: 445858); Flavonoids; Fractionation; Isoquercitrin (PubChem CID: 25203368); Lingonberry; Nrf2; Partridgeberry; Phloretin (PubChem CID: 4788); Phlorhizin (PubChem CID: 6072); Polyphenol; Quercetin (PubChem CID: 5280343); Vaccinium vitis-idaea