title: Anisotropic cellular network formation in engineered muscle tissue through the self-organization of neurons and endothelial cells.
abstract: Tissue anisotropy directed by cell sheets: Aligned myoblasts can be harvested as an anisotropic cell sheet using a micropatterned thermoresponsive substrate. Neurons and endothelial cells sandwiched between multiple anisotropic cell sheets self-organize oriented cellular networks in the tissue construct. This simple tissue engineering technique is useful for the creation of biomimetic microstructures in complex tissue, required for future advances in regenerative medicine.
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan.
authors.forename: Hironobu
authors.initials: H
authors.lastname: Takahashi
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Shimizu
authors.forename: Masamichi
authors.initials: M
authors.lastname: Nakayama
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D016880:Anisotropy; D002448:Cell Adhesion; D042783:Endothelial Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology; D018482:Muscle, Skeletal / Q000166:cytology* / Q000502:physiology; D032448:Myoblasts, Skeletal / Q000166:cytology; D009474:Neurons / Q000166:cytology*; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*
keywords: cell orientation; cell sheets; iPS cells; neurons; tissue engineering