title: Effect of apitherapy formulations against carbon tetrachloride-induced toxicity in Wistar rats after three weeks of treatment.
abstract: The human body is exposed nowadays to increasing attacks by toxic compounds in polluted air, industrially processed foods, alcohol and drug consumption that increase liver toxicity, leading to more and more severe cases of hepatic disorders. The present paper aims to evaluate the influence of the apitherapy diet in Wistar rats with carbon tetrachloride-induced hepatotoxicity, by analyzing the biochemical determinations (enzymatic, lipid and protein profiles, coagulation parameters, minerals, blood count parameters, bilirubin levels) and histopathological changes at the level of liver, spleen and pancreas. The experiment was carried out on six groups of male Wistar rats. Hepatic lesions were induced by intraperitoneal injection of carbon tetrachloride (dissolved in paraffin oil, 10% solution). Two mL per 100 g were administered, every 2 days, for 2 weeks. Hepatoprotection was achieved with two apitherapy diet formulations containing honey, pollen, propolis, Apilarnil, with/without royal jelly. Biochemical results reveal that the two apitherapy diet formulations have a positive effect on improving the enzymatic, lipid, and protein profiles, coagulation, mineral and blood count parameters and bilirubin levels. The histopathological results demonstrate the benefits of the two apitherapy diet formulations on reducing toxicity at the level of liver, spleen and pancreas in laboratory animals.
authors.affiliation: Apitherapy Medical Center, Balanesti, Nr. 336-337, Gorj 217036, Romania. marpopa2001@yahoo.fr.
authors.forename: Calin Vasile
authors.initials: CV
authors.lastname: Andritoiu
authors.affiliation: Grigore T. Popa University of Medicine and Pharmacy of Iasi, Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitatii Street, 16, Iasi 700115, Romania. ochiuzd@yahoo.com.
authors.forename: Lacramioara
authors.initials: L
authors.lastname: Ochiuz
authors.affiliation: Apitherapy Medical Center, Balanesti, Nr. 336-337, Gorj 217036, Romania. apitherapy2003@yahoo.com.
authors.forename: Vasile
authors.initials: V
authors.lastname: Andritoiu
authors.affiliation: Gheorghe Asachi Technical University of Iasi, Department of Natural and Synthetic Polymers, Iasi 700050, Romania. marpopa2001@yahoo.fr.
authors.forename: Marcel
authors.initials: M
authors.lastname: Popa
mesh_terms: D000284:Administration, Oral; D000818:Animals; D056705:Apitherapy; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury / Q000178:diet therapy* / Q000378:metabolism; D005227:Fatty Acids / Q000008:administration & dosage; D005526:Food, Formulated; D006722:Honey; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D017208:Rats, Wistar; D013154:Spleen / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013918:Thrombin Time; D000099219:Royal Jelly
chemical_list: D002251:Carbon Tetrachloride; D005227:Fatty Acids; D008055:Lipids; D000099219:Royal Jelly