title: Hox protein interactions: screening and network building.
abstract: Understanding the mode of action of Hox proteins requires the identification of molecular and cellular pathways they take part in. This includes to characterize the networks of protein-protein interactions involving Hox proteins. In this chapter we propose a strategy and methods to map Hox interaction networks, from yeast two-hybrid and high-throughput yeast two-hybrid interaction screening to bioinformatic analyses based on the software platform Cytoscape.
authors.affiliation: Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 4-5 box L7.07.10, Louvain-la-Neuve, 1348, Belgium.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bergiers
authors.forename: Barbara
authors.initials: B
authors.lastname: Lambert
authors.forename: Sarah
authors.initials: S
authors.lastname: Daakour
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Twizere
authors.forename: René
authors.initials: R
authors.lastname: Rezsohazy
mesh_terms: D019295:Computational Biology / Q000379:methods; D030562:Databases, Protein; D057166:High-Throughput Screening Assays; D018398:Homeodomain Proteins / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D060066:Protein Interaction Maps; D015203:Reproducibility of Results; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000378:metabolism
chemical_list: D018398:Homeodomain Proteins