title: Proteomic profiling reveals insights into Triticeae stigma development and function.
abstract: To our knowledge, this study represents the first high-throughput characterization of a stigma proteome in the Triticeae. A total of 2184 triticale mature stigma proteins were identified using three different gel-based approaches combined with mass spectrometry. The great majority of these proteins are described in a Triticeae stigma for the first time. These results revealed many proteins likely to play important roles in stigma development and pollen-stigma interactions, as well as protection against biotic and abiotic stresses. Quantitative comparison of the triticale stigma transcriptome and proteome showed poor correlation, highlighting the importance of having both types of analysis. This work makes a significant contribution towards the elucidation of the Triticeae stigma proteome and provides novel insights into its role in stigma development and function.
authors.affiliation: Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, 960 Carling Avenue, Ottawa, ON, Canada K1A 0C6 Carleton University, Department of Biology, 1125 Colonel By Drive, Ottawa, ON, Canada K1S 5B6.
authors.forename: Nazila
authors.initials: N
authors.lastname: Nazemof
authors.affiliation: Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, 960 Carling Avenue, Ottawa, ON, Canada K1A 0C6.
authors.forename: Philippe
authors.initials: P
authors.lastname: Couroux
authors.affiliation: Agriculture and Agri-Food Canada, Cereal Research Centre, 101 Route 100, Morden, MB, Canada R6M 1Y5.
authors.forename: Christof
authors.initials: C
authors.lastname: Rampitsch
authors.affiliation: Carleton University, Department of Biology, 1125 Colonel By Drive, Ottawa, ON, Canada K1S 5B6.
authors.forename: Tim
authors.initials: T
authors.lastname: Xing
authors.affiliation: Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, 960 Carling Avenue, Ottawa, ON, Canada K1A 0C6 Laurian.Robert@agr.gc.ca.
authors.forename: Laurian S
authors.initials: LS
authors.lastname: Robert
mesh_terms: D002523:Edible Grain / Q000254:growth & development / Q000378:metabolism*; D035264:Flowers / Q000254:growth & development / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000378:metabolism; D011058:Pollen / Q000254:growth & development / Q000378:metabolism; D020543:Proteome; D040901:Proteomics / Q000379:methods*
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: Electrophoresis; Triticeae.; mass spectrometry; proteomics; stigma; triticale