title: Sensitivity of Danio rerio (Teleostei, Cyprinidae) during two stages of development based on acute toxicity tests.
abstract: The sensitivity of Danio rerio to three chemicals was compared at two growth stages [larval (10 ± 2 after hatching) and post-larval (60 ± 4 days after hatching)] based on acute toxicity tests. Thirty-nine 48 h acute toxicity tests were performed with the substances CuSO4, NaCl and KCl. The 48 h LC50 values at the two growth stages were compared by independent samples t-tests. The results showed a clear decrease in sensitivity when post-larval organisms were used. Since acute toxicity test methods for D. rerio that recommend using post-larval stage fish do not represent the most sensitive stage of the test organism, our study suggests a revision of the methods to use larval fish.
authors.affiliation: Laboratory of Ecology and Conservation of Aquatic Ecosystems, University of Vale do Rio dos Sinos (UNISINOS), Unisinos Av., 950, São Leopoldo, RS, 93022-000, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Freiry
authors.forename: J A A
authors.initials: JA
authors.lastname: Stelzer
authors.forename: L
authors.initials: L
authors.lastname: Maltchik
authors.forename: A
authors.initials: A
authors.lastname: Arenzon
mesh_terms: D000367:Age Factors; D000818:Animals; D019327:Copper Sulfate / Q000633:toxicity*; D007814:Larva / Q000187:drug effects; D007928:Lethal Dose 50; D023421:Models, Animal; D011189:Potassium Chloride / Q000633:toxicity*; D012965:Sodium Chloride / Q000633:toxicity*; D023382:Toxicity Tests, Acute / Q000379:methods*; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D012965:Sodium Chloride; D011189:Potassium Chloride; D019327:Copper Sulfate