title: Effect of particle size distribution on the separation efficiency in liquid chromatography.
abstract: In this work, the influence of the width of particle size distribution (PSD) on chromatographic efficiency is studied. The PSD is described by lognormal distribution. A theoretical framework is developed in order to calculate heights equivalent to a theoretical plate in case of different PSDs. Our calculations demonstrate and verify that wide particle size distributions have significant effect on the separation efficiency of molecules. The differences of fully porous and core-shell phases regarding the influence of width of PSD are presented and discussed. The efficiencies of bimodal phases were also calculated. The results showed that these packings do not have any advantage over unimodal phases.
authors.affiliation: Department of Analytical Chemistry, University of Pannonia, Egyetem utca 10, H-8200 Veszprém, Hungary. Electronic address: raksi@almos.uni-pannon.hu.
authors.forename: Krisztián
authors.initials: K
authors.lastname: Horváth
authors.affiliation: Department of Analytical Chemistry, University of Pannonia, Egyetem utca 10, H-8200 Veszprém, Hungary.
authors.forename: Diána
authors.initials: D
authors.lastname: Lukács
authors.affiliation: MTA-PTE Molecular Interactions in Separation Science Research Group, Ifjúság útja 6, H-7624 Pécs, Hungary.
authors.forename: Annamária
authors.initials: A
authors.lastname: Sepsey
authors.affiliation: MTA-PTE Molecular Interactions in Separation Science Research Group, Ifjúság útja 6, H-7624 Pécs, Hungary; Department of Analytical and Environmental Chemistry and Szentágothai Research Center, University of Pécs, Ifjúság útja 6, H-7624 Pécs, Hungary.
authors.forename: Attila
authors.initials: A
authors.lastname: Felinger
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D010316:Particle Size; D016062:Porosity
keywords: Bimodal phases; Core–shell phases; General rate model; Particle size distribution; Separation efficiency