title: Identification, classification, and discrimination of agave syrups from natural sweeteners by infrared spectroscopy and HPAEC-PAD.
abstract: Agave syrups are gaining popularity as new natural sweeteners. Identification, classification and discrimination by infrared spectroscopy coupled to chemometrics (NIR-MIR-SIMCA-PCA) and HPAEC-PAD of agave syrups from natural sweeteners were achieved. MIR-SIMCA-PCA allowed us to classify the natural sweeteners according to their natural source. Natural syrups exhibited differences in the MIR spectra region 1500-900 cm(-1). The agave syrups displayed strong absorption in the MIR spectra region 1061-1,063 cm(-1), in agreement with their high fructose content. Additionally, MIR-SIMCA-PCA allowed us to differentiate among syrups from different Agave species (Agavetequilana and Agavesalmiana). Thin-layer chromatography and HPAEC-PAD revealed glucose, fructose, and sucrose as the principal carbohydrates in all of the syrups. Oligosaccharide profiles showed that A. tequilana syrups are mainly composed of fructose (>60%) and fructooligosaccharides, while A. salmiana syrups contain more sucrose (28-32%). We conclude that MIR-SIMCA-PCA and HPAEC-PAD can be used to unequivocally identify and classified agave syrups.
authors.affiliation: Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Apartado Postal 629, Irapuato, Gto. CP 36821, Mexico.
authors.forename: Erika
authors.initials: E
authors.lastname: Mellado-Mojica
authors.affiliation: Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Apartado Postal 629, Irapuato, Gto. CP 36821, Mexico. Electronic address: mlopez@ira.cinvestav.mx.
authors.forename: Mercedes G
authors.initials: MG
authors.lastname: López
mesh_terms: D027882:Agave / Q000737:chemistry*; D005632:Fructose / Q000032:analysis*; D009844:Oligosaccharides / Q000032:analysis*; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013549:Sweetening Agents / Q000032:analysis*
chemical_list: D009844:Oligosaccharides; D013549:Sweetening Agents; D005632:Fructose
keywords: Agave syrups; Chemometrics; Fructooligosaccharides; HPAEC-PAD; MIR-NIR-PCA; Natural sweeteners