title: [MiR-425 up-regulation induced by interleukin-1β promotes the proliferation of gastric cancer cell AGS].
abstract: OBJECTIVE
  To evaluate the mechanism of interleukin-1 (IL-1) in tumorigenesis.
  
  
  METHODS
  The miRNA expression was profiled with the Exiqon miRCURY(TM) LNA Array System. And the miRecords algorithm and luciferase reporter assays were employed for identifying the targets of miR-425. Cell proliferation assay was performed with CCK8. Cell apoptosis assay was performed with flow cytometry.
  
  
  RESULTS
  IL-1β induced the up-regulation of miR-425, a negative expression regulator of phosphatase and tensin homolog (PTEN). An increase in miR-425 depended upon IL-1β-induced NF-kappaB activation.Repression of PTEN by miR-425 promoted gastric cancer cell proliferation.
  
  
  CONCLUSIONS
  There is a critical role for NF-kappa B-dependent up-regulation of miR-425. And it represents a new pathway for the repression of phosphatase and tensin homolog activation and the promotion of cell survival upon IL-1β induction.
authors.affiliation: Department of Integrative Medicine, Affiliated Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Tianyu
authors.initials: T
authors.lastname: Li
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Yang
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.forename: Xixi
authors.initials: X
authors.lastname: Gu
authors.forename: Yue
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Email: xiaoniulj@126.com.
authors.forename: Dingfang
authors.initials: D
authors.lastname: Cai
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D005786:Gene Expression Regulation; D006801:Humans; D053583:Interleukin-1beta; D035683:MicroRNAs; D016328:NF-kappa B; D051059:PTEN Phosphohydrolase; D013274:Stomach Neoplasms; D015854:Up-Regulation
chemical_list: D053583:Interleukin-1beta; D035683:MicroRNAs; D016328:NF-kappa B; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human