title: Technologies and strategies to characterize and quantitate metabolites in drug discovery and development.
abstract: Pharmacokinetics and toxicokinetics studies rely on the quantitation of drugs and its metabolites. Drug metabolism studies are based on the characterization of the structure of drugs and their metabolites. Liquid chromatography coupled with mass spectrometry is an undisputed technology for the routine analysis of drugs and metabolites in the drug discovery set-up. Advancements in liquid chromatography and mass spectrometry have accelerated and improved drug discovery and development. Identification and quantitation of drugs and metabolites have gained importance because of their relevance to clinical research. In the present review, we present a bird's eye view of qualitative and quantitative analysis of drugs and their metabolites. Various technologies available to characterize and quantitate metabolites are analyzed. Characterization of metabolites of the sulfonyl urea drug glyburide and the kinetics of glyburide metabolism are discussed in detail.
authors.affiliation: Departments of Bio-Analytical and Biotransformation, Drug Metabolism and Pharmacokinetics, Sai Life Sciences Limited, Building 1, Plot No 2, Chrysalis Enclave, International Bio-Tech Park, Phase-II, Hinjewadi, Pune-, 411 057, Maharashtra, India.
authors.forename: Selvan
authors.initials: S
authors.lastname: Ravindran
authors.forename: Amol
authors.initials: A
authors.lastname: Jadhav
authors.forename: Prashant
authors.initials: P
authors.lastname: Surve
authors.forename: Ganesh
authors.initials: G
authors.lastname: Lonsane
authors.forename: Pradnya
authors.initials: P
authors.lastname: Honrao
authors.forename: Bidyut
authors.initials: B
authors.lastname: Nanda
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D055808:Drug Discovery / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
chemical_list: D004364:Pharmaceutical Preparations
keywords: drug discovery; liquid chromatography; mass spectrometry; metabolites; technologies