title: Metabolomic analysis of platelets during storage: a comparison between apheresis- and buffy coat-derived platelet concentrates.
abstract: BACKGROUND
  Platelet concentrates (PCs) can be prepared using three methods: platelet (PLT)-rich plasma, apheresis, and buffy coat. The aim of this study was to obtain a comprehensive data set that describes metabolism of buffy coat-derived PLTs during storage and to compare it with a previously published parallel data set obtained for apheresis-derived PLTs.
  
  
  METHODS
  During storage we measured more than 150 variables in 8 PLT units, prepared by the buffy coat method. Samples were collected at seven different time points resulting in a data set containing more than 8000 measurements. This data set was obtained by combining a series of standard quality control assays to monitor the quality of stored PLTs and a deep coverage metabolomics study using liquid chromatography coupled with mass spectrometry.
  
  
  RESULTS
  Stored PLTs showed a distinct metabolic transition occurring 4 days after their collection. The transition was evident in PLT produced by both production methods. Apheresis-derived PLTs showed a clearer phenotype of PLT activation during early days of storage. The activated phenotype of apheresis PLTs was accompanied by a higher metabolic activity, especially related to glycolysis and the tricarboxylic acid cycle. Moreover, the extent of the activation differed between bags resulting in interbag variability in the storage lesion of apheresis-prepared PLTs. This may be related to donor-related polymorphism.
  
  
  CONCLUSIONS
  This study demonstrated two discrete metabolic phenotypes in stored PLTs prepared with both apheresis and buffy coat methods. PLT activation occurs during the first metabolic phenotype and might lead to a low reproducibility of the apheresis PCs.
authors.affiliation: Center for Systems Biology, University of Iceland, Reykjavik, Iceland.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Paglia
authors.forename: Ólafur E
authors.initials: ÓE
authors.lastname: Sigurjónsson
authors.forename: Óttar
authors.initials: Ó
authors.lastname: Rolfsson
authors.forename: Morten Bagge
authors.initials: MB
authors.lastname: Hansen
authors.forename: Sigurður
authors.initials: S
authors.lastname: Brynjólfsson
authors.forename: Sveinn
authors.initials: S
authors.lastname: Gudmundsson
authors.forename: Bernhard O
authors.initials: BO
authors.lastname: Palsson
mesh_terms: D000328:Adult; D058621:Blood Buffy Coat; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D001793:Blood Preservation; D005260:Female; D006801:Humans; D008297:Male; D055442:Metabolome; D055432:Metabolomics; D015539:Platelet Activation; D010983:Plateletpheresis; D013997:Time Factors