title: Different metabolic profiles of K1 serotype and non-serotype K1 and K2 Klebsiella pneumoniae isolates in oral infection mice model.
abstract: K1 or K2 serotype Klebsiella pneumoniae isolate caused clinical pyogenic liver abscess (KLA) infection is prevalent in many areas. It has been identified that K1 or K2 serotype K. pneumoniae isolates caused KLA infection in mice by oral inoculation. In our study, K1 serotype K. pneumoniae isolate Kp1002 with hypermucoviscosity (HV)-positive phenotype caused KLA infection in C57BL/6 mice by oral inoculation. Simultaneously, non-serotype K1 and K2 isolate Kp1014 with HV-negative phenotype failed to cause KLA infection in the same manner. It seems that gastrointestinal tract translocation is the pathway by which K1 or K2 serotype K. pneumoniae caused KLA infection. Liquid chromatography-tandem mass spectrometry was used to further analyze metabolic profile changes in mice with KLA infection. Data showed that after Kp1002 or Kp1014 oral inoculation, serum Phosphatidylcholine (PC) and Lysophosphatidylcholine (LPC) levels significantly changed in mice. Some PC and LPC molecules showed changes both in the Kp1002 KLA group and the Kp1014 no-KLA group compared with the control group. The level of 18:1/18:2-PC significantly changed in the Kp1002 KLA group compared with the control group, but showed no change between the Kp1014 no-KLA group and the control group. The level of 18:1/18:2-PC might have been particularly affected by KLA infection caused by K1 serotype K. pneumoniae Kp1002. It may be a potential biomarker for KLA infection.
authors.affiliation: Department of Medical Microbiology, Medicine College, Hebei University, Baoding 071000, China. Electronic address: cnady@126.com.
authors.forename: Nan
authors.initials: N
authors.lastname: Chen
authors.affiliation: Department of Basic Medical Sciences, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Wang
authors.affiliation: Basic Medical Sciences, Medicine College, Hebei University, Baoding 071000, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Xue
authors.affiliation: Department of Medical Microbiology, Medicine College, Hebei University, Baoding 071000, China.
authors.forename: Xiang-Bo
authors.initials: XB
authors.lastname: Ma
authors.affiliation: Department of Medical Microbiology, Medicine College, Hebei University, Baoding 071000, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Basic Medical Sciences, Medicine College, Hebei University, Baoding 071000, China.
authors.forename: Rui-Xue
authors.initials: RX
authors.lastname: Rong
authors.affiliation: Department of Medical Microbiology, Medicine College, Hebei University, Baoding 071000, China.
authors.forename: Hong-Quan
authors.initials: HQ
authors.lastname: Li
authors.affiliation: Basic Medical Sciences, Medicine College, Hebei University, Baoding 071000, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Ding
authors.affiliation: Department of Pharmacology, Zhejiang Medical College, Hangzhou 310053, China.
authors.forename: Ming-Zhi
authors.initials: MZ
authors.lastname: Zheng
authors.affiliation: Department of Basic Medical Sciences, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Chen
authors.affiliation: Basic Medical Sciences, Medicine College, Hebei University, Baoding 071000, China. Electronic address: feiduan9999@sina.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Duan
authors.affiliation: Department of Basic Medical Sciences, Zhejiang University School of Medicine, Hangzhou 310058, China. Electronic address: shenyueliang@zju.edu.cn.
authors.forename: Yue-Liang
authors.initials: YL
authors.lastname: Shen
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D016667:Bacterial Capsules / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D002853:Chromatography, Liquid; D004195:Disease Models, Animal; D007710:Klebsiella Infections / Q000382:microbiology* / Q000473:pathology*; D007711:Klebsiella pneumoniae / Q000145:classification / Q000302:isolation & purification*; D008100:Liver Abscess / Q000382:microbiology* / Q000473:pathology*; D008244:Lysophosphatidylcholines / Q000097:blood; D008297:Male; D055442:Metabolome; D055432:Metabolomics; D008810:Mice, Inbred C57BL; D010713:Phosphatidylcholines / Q000097:blood; D011135:Polysaccharides, Bacterial / Q000276:immunology; D065288:Serogroup; D053719:Tandem Mass Spectrometry
chemical_list: D000942:Antigens, Bacterial; D015415:Biomarkers; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D011135:Polysaccharides, Bacterial; C036962:capsular polysaccharide K1; C068924:capsular polysaccharide K2
keywords: Gastrointestinal tract translocation; K1 serotype Klebsiella pneumoniae; LC-MS; Liver abscess; Metabolomics