title: Chromatin immunoprecipitation and chromatin immunoprecipitation with massively parallel sequencing on mouse embryonic tissue.
abstract: Regulation of gene expression must be tightly controlled during embryonic development. A central mechanism to control gene expression is the binding of sequence-specific transcription factors to cis-regulatory elements in the genome. Chromatin immunoprecipitation (ChIP) is a widely used technique to analyze binding of transcription factors and histone modifications on chromatin; however, it is limited to looking at a small number of genes. ChIP with massively parallel sequencing (ChIP-seq) is a recently developed powerful tool to analyze genome-wide binding of transcription factors and histone modifications and provides a vast amount of information into the regulation of gene expression. This chapter describes how ChIP and ChIP-seq are performed on mouse embryonic tissue.
authors.affiliation: School of Dentistry, Faculty of Medical & Human Sciences, University of Manchester, Manchester, M13 9PT, UK.
authors.forename: Shilu
authors.initials: S
authors.lastname: Amin
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Bobola
mesh_terms: D000818:Animals; D002843:Chromatin / Q000378:metabolism; D047369:Chromatin Immunoprecipitation / Q000379:methods; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D051379:Mice; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D002843:Chromatin; D014157:Transcription Factors; D004247:DNA