title: Age-dependent redistribution and hypersialylation of the central myelin paranodal loop membrane protein Opalin in the mouse brain.
abstract: Opalin/Tmem10 is a myelin-associated sialylglycoprotein that is specific to only the mammalian central nervous system. However, little is known about the properties or function of this protein. Here, we analyzed the expression and glycosylation patterns of Opalin in the postnatal mouse brain. Immunolocalization patterns of Opalin were similar to those of myelin basic protein in juvenile and adolescent mice. On the other hand, in the adult mouse brain, decreasing immunoreactivity for Opalin was observed in the hindbrain region especially in the cerebellar white matter compared with the corpus callosum in the forebrain. In addition, Opalin showed increasing molecular size with mouse aging. This age-dependent increase in Opalin molecular weight was mainly owing to hypersialylation of O-glycans. These results indicate that the regional redistribution and the degree of sialylation of Opalin protein are age-dependently regulated in mouse brains.
authors.affiliation: Laboratory for Molecular Neurogenesis, RIKEN Brain Science Institute, Saitama 351-0198, Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Laboratory for Molecular Neurogenesis, RIKEN Brain Science Institute, Saitama 351-0198, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Yoshikawa
authors.affiliation: Laboratory for Molecular Neurogenesis, RIKEN Brain Science Institute, Saitama 351-0198, Japan; Advanced Scientific Research Leaders Development Unit, Gunma University, Gunma 371-8511, Japan.
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Sadakata
authors.affiliation: Laboratory for Molecular Neurogenesis, RIKEN Brain Science Institute, Saitama 351-0198, Japan; Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Chiba 278-8510, Japan.
authors.forename: Yo
authors.initials: Y
authors.lastname: Shinoda
authors.affiliation: Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Chiba 278-8510, Japan.
authors.forename: Michinori
authors.initials: M
authors.lastname: Koebis
authors.affiliation: Laboratory for Molecular Neurogenesis, RIKEN Brain Science Institute, Saitama 351-0198, Japan; Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Chiba 278-8510, Japan. Electronic address: tfuruichi@rs.tus.ac.jp.
authors.forename: Teiichi
authors.initials: T
authors.lastname: Furuichi
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D006031:Glycosylation; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D004676:Myelin Basic Protein / Q000032:analysis; D009185:Myelin Proteins / Q000032:analysis* / Q000378:metabolism
chemical_list: D004676:Myelin Basic Protein; D009185:Myelin Proteins; C524019:Opalin protein, mouse
keywords: Myelin; Oligodendrocyte; Opalin; Paranodal loops; Sialylated glycoprotein; Tmem10