title: Relationship between nanoscale mineral properties and calcein labeling in mineralizing bone surfaces.
abstract: Bone's mineral properties, such as particle thickness and degree of alignment have been associated with bone quality. Bone formation, remodeling, aging of the tissue and mineral homeostasis influence mineral particle properties leading to specific patterns across bone. Scanning small angle X-ray scattering (sSAXS) with synchrotron radiation is a powerful tool, which allows us to study bone's nanoscale mineral properties in a position-resolved way. We used sSAXS, fluorescence light microscopy and backscattered electron (BSE) imaging to study bone's mineral properties at the tibial midshaft of in vivo-loaded mice. By combining these techniques, we could detect local changes in mineral properties. Regions labeled with calcein fluorochrome have lower mean mineral thickness and degree of mineral alignment. We also observed thinner and less aligned mineral particles near blood vessels. We conclude that mineral properties (i) are altered by fluorochrome labeling and (ii) depend on the proximity to blood vessels.
authors.affiliation: Julius Wolff Institut, Charité-Universitätsmedizin , Berlin , Germany .
authors.forename: Marta
authors.initials: M
authors.lastname: Aido
authors.forename: Michael
authors.initials: M
authors.lastname: Kerschnitzki
authors.forename: Rebecca
authors.initials: R
authors.lastname: Hoerth
authors.forename: Manfred
authors.initials: M
authors.lastname: Burghammer
authors.forename: Cédric
authors.initials: C
authors.lastname: Montero
authors.forename: Sara
authors.initials: S
authors.lastname: Checa
authors.forename: Peter
authors.initials: P
authors.lastname: Fratzl
authors.forename: Georg N
authors.initials: GN
authors.lastname: Duda
authors.forename: Bettina M
authors.initials: BM
authors.lastname: Willie
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wagermaier
mesh_terms: D000375:Aging; D000818:Animals; D001842:Bone and Bones / Q000648:ultrastructure*; D002113:Calcification, Physiologic / Q000502:physiology*; D005260:Female; D005452:Fluoresceins / Q000737:chemistry; D008810:Mice, Inbred C57BL; D049329:Nanostructures; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D005452:Fluoresceins; C007740:fluorexon
keywords: Bone mineral; calcein; mice; synchrotron sSAXS