title: Evaluation of hemostatic and anti-inflammatory activities of extracts from different Lagochilus species in experimental animals: comparison of different extractives and sources.
abstract: Different members of Lagochilus genus have been used in folkloric medicine to treat hemorrhages and inflammation. However, only a few species of them have received scientific attention supporting their efficacy. Here, the hemostatic and antiinflammatory activities of five Lagochilus species were determined and compared by using in vivo assays. The results showed that the extracts of Lagochilus lanatonodus and Lagochilus diacanthophyllus showed better hemostatic activities among five species. The high doses of L. lanatonodus extracts were able to shorten the values of thrombin time, activated partial thromboplastin time and prothrombin time in a rat model. Moreover, the extracts of L. lanatonodus and L. diacanthophyllus showed strong inhibitory effects on the acute phase of inflammation in both xylene-induced ear edema mouse model and carrageenan-induced paw edema rat model. In parallel, the treatment of these extracts modulated the expressions of those inflammatory parameters, that is, nitric oxide, prostaglandin E2 , inducible nitric oxide synthase, malondialdehyde and superoxide dismutase. L. lanatonodus and L. diacanthophyllus showed better hemostatic and antiinflammatory activities in several test models: these results therefore supported the folkloric utilization. L. lanatonodus was found to be the most active Lagochilus species.
authors.affiliation: The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicines, The State Administration of TCM (SATCM) Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Shanghai Key Laboratory of Complex Prescription, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China; Shanghai R&D Center for Standardization of Chinese Medicines, Shanghai, 201203, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Jiao
authors.forename: Pei-Hua
authors.initials: PH
authors.lastname: Chen
authors.forename: Ai-Zhen
authors.initials: AZ
authors.lastname: Xiong
authors.forename: Zheng-Tao
authors.initials: ZT
authors.lastname: Wang
authors.forename: Karl Wah-Keung
authors.initials: KW
authors.lastname: Tsim
authors.forename: Gui-Xin
authors.initials: GX
authors.lastname: Chou
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002351:Carrageenan / Q000009:adverse effects; D015232:Dinoprostone / Q000378:metabolism; D004487:Edema / Q000188:drug therapy; D006490:Hemostatics / Q000627:therapeutic use*; D007249:Inflammation / Q000188:drug therapy*; D019686:Lamiaceae / Q000737:chemistry* / Q000145:classification; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D023382:Toxicity Tests, Acute
chemical_list: D000893:Anti-Inflammatory Agents; D006490:Hemostatics; D010936:Plant Extracts; D009569:Nitric Oxide; D008315:Malondialdehyde; D002351:Carrageenan; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D013482:Superoxide Dismutase; D015232:Dinoprostone
keywords: Lagochilus species; antiinflammatory; hemostatic; traditional herbal medicine