title: Anticancer effects of brucine and gemcitabine combination in MCF-7 human breast cancer cells.
abstract: This study was designed to investigate the combination effects of brucine and gemcitabine, each with anticancer properties, in MCF-7 human breast cancer cells in culture. With regard to cell viability, effects of both the drugs and their combinations were inversely proportional to dose and time. For various proportional drug combinations studied, combination effects were analysed using CompuSyn software. The analyses revealed synergistic and/or additive effects regarding cell viability, anchorage-independent growth and cell migration. Combination analyses exhibited diversified impacts of the type of combination treatment, namely pretreatment with either drug followed by exposure to the other, or treatment with both drugs at the same time. Compared with untreated cells, combination treatment of asynchronised MCF-7 cells resulted in 17.2 × decrease in G2 phase, increasing G1 (2.1 × ) and S (1.5 × ) phase cells in cell cycle analysis. Brucine, either individually or in combination, but not gemcitabine, inhibited NF-kB subunit (p65) expression in MCF-7 cells.
authors.affiliation: a Department of Biochemistry , S. V. University , Sugen Life Sciences Pvt Ltd, #4/86, S.V. Nagar, Perumalla Palli, Tirupati 517505 , Andhra Pradesh , India.
authors.forename: Mamatha
authors.initials: M
authors.lastname: Serasanambati
authors.forename: Shanmuga Reddy
authors.initials: SR
authors.lastname: Chilakapati
authors.forename: Pavan Kumar
authors.initials: PK
authors.lastname: Manikonda
authors.forename: Jagadeeswara Reddy
authors.initials: JR
authors.lastname: Kanala
authors.forename: Damodar Reddy
authors.initials: DR
authors.lastname: Chilakapati
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004359:Drug Therapy, Combination; D005260:Female; D006801:Humans; D061986:MCF-7 Cells / Q000187:drug effects; D013331:Strychnine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000093542:Gemcitabine
chemical_list: D000964:Antimetabolites, Antineoplastic; D003841:Deoxycytidine; C083806:brucine; D013331:Strychnine; D000093542:Gemcitabine
keywords: NF-kB; breast cancer; brucine; cell cycle; combination; gemcitabine; synergy