title: Characterization of glial cell models and in vitro manipulation of the neuregulin1/ErbB system.
abstract: The neuregulin1/ErbB system plays an important role in Schwann cell behavior both in normal and pathological conditions. Upon investigation of the expression of the neuregulin1/ErbB system in vitro, we explored the possibility to manipulate the system in order to increase the migration of Schwann cells, that play a fundamental role in the peripheral nerve regeneration. Comparison of primary cells and stable cell lines shows that both primary olfactory bulb ensheathing cells and a corresponding cell line express ErbB1-ErbB2 and neuregulin1, and that both primary Schwann cells and a corresponding cell line express ErbB2-ErbB3, while only primary Schwann cells express neuregulin1. To interfere with the neuregulin1/ErbB system, the soluble extracellular domain of the neuregulin1 receptor ErbB4 (ecto-ErbB4) was expressed in vitro in the neuregulin1 expressing cell line, and an unexpected increase in cell motility was observed. In vitro experiments suggest that the back signaling mediated by the transmembrane neuregulin1 plays a role in the migratory activity induced by ecto-ErbB4. These results indicate that ecto-ErbB4 could be used in vivo as a tool to manipulate the neuregulin1/ErbB system.
authors.affiliation: Department of Clinical and Biological Sciences, University of Torino, Nerve Regeneration Group, 10043 Orbassano, Italy.
authors.forename: Davide
authors.identifier: 0000-0003-1089-5736
authors.initials: D
authors.lastname: Pascal
authors.affiliation: Department of Clinical and Biological Sciences, University of Torino, Nerve Regeneration Group, 10043 Orbassano, Italy.
authors.forename: Alessia
authors.identifier: 0000-0002-0765-298X
authors.initials: A
authors.lastname: Giovannelli
authors.affiliation: Department of Clinical and Biological Sciences, University of Torino, Nerve Regeneration Group, 10043 Orbassano, Italy ; Neuroscience Institute Cavalieri Ottolenghi (NICO), 10043 Orbassano, Italy.
authors.forename: Sara
authors.identifier: 0000-0002-3230-7424
authors.initials: S
authors.lastname: Gnavi
authors.affiliation: Department of Neuroregeneration, Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences (KNAW), 1105 BA Amsterdam, The Netherlands ; Department of Neurosurgery, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands.
authors.forename: Stefan Adriaan
authors.identifier: 0000-0001-8371-3871
authors.initials: SA
authors.lastname: Hoyng
authors.affiliation: Department of Neuroregeneration, Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences (KNAW), 1105 BA Amsterdam, The Netherlands ; Department of Neurosurgery, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands.
authors.forename: Fred
authors.identifier: 0000-0002-3042-2429
authors.initials: F
authors.lastname: de Winter
authors.affiliation: Department of Clinical and Biological Sciences, University of Torino, Nerve Regeneration Group, 10043 Orbassano, Italy.
authors.forename: Michela
authors.identifier: 0000-0001-6800-1139
authors.initials: M
authors.lastname: Morano
authors.affiliation: Department of Clinical and Biological Sciences, University of Torino, Nerve Regeneration Group, 10043 Orbassano, Italy.
authors.forename: Federica
authors.identifier: 0000-0001-6520-4897
authors.initials: F
authors.lastname: Fregnan
authors.affiliation: Institute of Neurological Sciences, National Research Council (CNR), 95126 Catania, Italy.
authors.forename: Paola
authors.identifier: 0000-0003-4349-0276
authors.initials: P
authors.lastname: Dell'Albani
authors.affiliation: Department of Experimental Medicine, Section of Anatomy, University of Genova, 16132 Genova, Italy.
authors.forename: Damiano
authors.identifier: 0000-0001-8804-6043
authors.initials: D
authors.lastname: Zaccheo
authors.affiliation: Department of Clinical and Biological Sciences, University of Torino, Nerve Regeneration Group, 10043 Orbassano, Italy ; Neuroscience Institute of Torino (NIT), Interdepartmental Centre of Advanced Studies in Neuroscience, University of Torino, 10043 Orbassano, Italy.
authors.forename: Isabelle
authors.identifier: 0000-0001-9797-2276
authors.initials: I
authors.lastname: Perroteau
authors.affiliation: Institute of Neurological Sciences, National Research Council (CNR), 95126 Catania, Italy.
authors.forename: Rosalia
authors.identifier: 0000-0002-1551-5467
authors.initials: R
authors.lastname: Pellitteri
authors.affiliation: Department of Clinical and Biological Sciences, University of Torino, Nerve Regeneration Group, 10043 Orbassano, Italy ; Neuroscience Institute of Torino (NIT), Interdepartmental Centre of Advanced Studies in Neuroscience, University of Torino, 10043 Orbassano, Italy.
authors.forename: Giovanna
authors.identifier: 0000-0002-8380-5925
authors.initials: G
authors.lastname: Gambarotta
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology*; D020890:Neuregulin-1 / Q000378:metabolism*; D051381:Rats; D066247:Receptor, ErbB-4 / Q000378:metabolism*; D012583:Schwann Cells / Q000166:cytology* / Q000502:physiology*
chemical_list: D020890:Neuregulin-1; C490580:Nrg1 protein, rat; D066247:Receptor, ErbB-4