title: MicroRNA-320a inhibits cell proliferation, migration and invasion by targeting BMI-1 in nasopharyngeal carcinoma.
abstract: In the present study, we investigated the roles and molecular mechanisms of miR-320a in human nasopharyngeal carcinoma (NPC). miR-320a expression was strongly reduced in NPC tissues and cell lines. Overexpression of miR-320a significantly suppressed NPC cell growth, migration, invasion and tumor growth in a xenograft mouse model. A luciferase reporter assay revealed that miR-320a could directly bind to the 3' UTR of BMI-1. Overexpression of BMI-1 rescued miR-320a-mediated biological function. BMI-1 expression was found to be up-regulated and inversely correlated with miR-320a expression in NPC. Collectively, our data indicate that miR-320a plays a tumor suppressor role in the development and progression of NPC and may be a novel therapeutic target against NPC.
authors.affiliation: Department of E.N.T., The 88th Hospital of PLA, Tai'an, Shandong 271000, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Qi
authors.affiliation: Department of E.N.T., The 88th Hospital of PLA, Tai'an, Shandong 271000, China.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of E.N.T., The 88th Hospital of PLA, Tai'an, Shandong 271000, China.
authors.forename: Changbo
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Department of E.N.T., The 88th Hospital of PLA, Tai'an, Shandong 271000, China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Lv
authors.affiliation: Department of Orthopedics, The 88th Hospital of PLA, Tai'an, Shandong 271000, China. Electronic address: tianminpla88@126.com.
authors.forename: Min
authors.initials: M
authors.lastname: Tian
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology; D063150:Polycomb Repressive Complex 1 / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D020413:3' Untranslated Regions; C069077:BMI1 protein, human; C572916:MIRN320 microRNA, human; D035683:MicroRNAs; D063150:Polycomb Repressive Complex 1
keywords: BMI-1; Invasion; Nasopharyngeal carcinoma; Proliferation; miR-320a