title: Establishment of a novel diagnostic model for Sjögren's syndrome by proteomic fingerprinting.
abstract: Primary Sjögren's syndrome (pSS) is a systemic autoimmune disease that lacks sensitive and specific diagnostic methods. The aim of this study was to identify potential biomarkers specific for pSS and to establish a diagnostic model. Serum samples from patients with pSS, disease controls (DC, patients with systemic lupus erythematosus (SLE), rheumatoid arthritis (RA)), and healthy controls (HC)) were randomly divided into a training set (35 pSS, 50 DC, and 26 HC) and a testing set (25 pSS, 50 DC, and 25 HC). Weak cationic exchange (WCX) magnetic beads were used to differentially capture serum proteins prior to proteomic analysis. Proteomic mass spectra were generated by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). One hundred differential M/Z peaks associated with pSS were identified, and the m/z peaks at 8,133.85, 11,972.8, 2,220.81, and 4,837.66 were used to establish a diagnostic model for pSS. This diagnostic model was able to distinguish pSS from non-pSS controls with a sensitivity of 77.1 % and a specificity of 85.5 %, and its efficacy was confirmed in our blinded testing set with good sensitivity and specificity of 95.5 and 88 %, respectively. The results indicated that the proteomic fingerprinting model was effective in the diagnosis of pSS.
authors.affiliation: Department of Rheumatology and Immunology, Clinical Immunology Center, Peking University People's Hospital, 11 Xizhimen South Street, 100044, Beijing, China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Sun
authors.forename: Xuewu
authors.initials: X
authors.lastname: Zhang
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Yang
authors.forename: Rulin
authors.initials: R
authors.lastname: Jia
authors.forename: Xu
authors.initials: X
authors.lastname: Liu
authors.forename: Ru
authors.initials: R
authors.lastname: Li
authors.forename: Yanying
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhanguo
authors.initials: Z
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000974:Antibodies, Antinuclear / Q000737:chemistry; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000175:diagnosis; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000737:chemistry; D016022:Case-Control Studies; D002412:Cations; D002852:Chromatography, Ion Exchange; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000175:diagnosis; D008280:Magnetics; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000737:chemistry; D023302:Protein Footprinting / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D012372:ROC Curve; D012680:Sensitivity and Specificity; D012859:Sjogren's Syndrome / Q000097:blood* / Q000175:diagnosis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055815:Young Adult
chemical_list: D000974:Antibodies, Antinuclear; D015415:Biomarkers; D001798:Blood Proteins; D002412:Cations; D010455:Peptides