title: Critical role of the GM-CSF signaling pathway in macrophage pro-repair activities.
abstract: OBJECTIVE
  Macrophages play a critical role in intestinal wound repair. However, the molecular pathways that regulate macrophage wound repair activities remain poorly understood. The aim of this study was to evaluate the role of GM-CSF receptor signaling in the wound repair activities of macrophages.
  
  
  METHODS
  Murine macrophages were differentiated from bone marrow cells and human macrophages from monocytes isolated from peripheral blood mononuclear cells of Crohn's disease (CD) patients. In vitro models were used to study the repair activities of macrophages.
  
  
  RESULTS
  We provide evidence that GM-CSF receptor signaling is required for murine macrophages to promote epithelial repair. In addition, we demonstrate that the deficient repair properties of macrophages from CD patients with active disease can be recovered via GM-CSF therapy.
  
  
  CONCLUSIONS
  Our data support a critical role of the GM-CSF signaling pathway in the pro-repair activities of mouse and human macrophages.
authors.affiliation: Service of Gastroenterology and Hepatology, Department of Medicine, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland.
authors.forename: Eric
authors.initials: E
authors.lastname: Bernasconi
authors.forename: Fabrizia
authors.initials: F
authors.lastname: D'Angelo
authors.forename: Pierre
authors.initials: P
authors.lastname: Michetti
authors.forename: Dominique
authors.initials: D
authors.lastname: Velin
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology; D018938:Caco-2 Cells; D003424:Crohn Disease / Q000378:metabolism; D004847:Epithelial Cells / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D006801:Humans; D008264:Macrophages / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000276:immunology; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D015398:Signal Transduction; D014945:Wound Healing
chemical_list: D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor