title: Preparation of Au and Ag nanoparticles using Artemisia annua and their in vitro antibacterial and tyrosinase inhibitory activities.
abstract: This work describes a plant-mediated approach to the preparation of metal nanoparticles using leaf extract of Artemisia annua (A. annua), an ethno-medicinal plant widely found in Asia, which was used as reducing and stabilizing agent. A. annua is used in traditional Chinese medicine to alleviate fever. Au and Ag nanoparticles were prepared using a one-step aqueous method at room temperature without any toxic chemicals. The formation of Au and Ag nanoparticles was monitored by UV-vis spectroscopy. Synthesized nanoparticles were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), energy dispersive X-ray analysis (EDX), Fourier transform infrared (FT-IR) spectroscopy, and thermogravimetric analysis (TGA). TEM analysis of Au nanoparticles showed that they had triangular and spherical shapes with sizes ranging from 15 to 40 nm. The silver nanoparticles were predominantly spherical and uniformly sized (30-50 nm). The Au and Ag nanoparticles produced showed significant tyrosinase inhibitory and antibacterial effects. These results suggest that the synthesized nanoparticles provide good alternatives in varied medical and industrial applications.
authors.affiliation: School of Chemical Engineering, Yeungnam University, Gyeongsan 712-749, Republic of Korea.
authors.forename: Nagaraj
authors.initials: N
authors.lastname: Basavegowda
authors.affiliation: School of Chemical Engineering, Yeungnam University, Gyeongsan 712-749, Republic of Korea.
authors.forename: Akber
authors.initials: A
authors.lastname: Idhayadhulla
authors.affiliation: School of Chemical Engineering, Yeungnam University, Gyeongsan 712-749, Republic of Korea. Electronic address: yrlee@yu.ac.kr.
authors.forename: Yong Rok
authors.initials: YR
authors.lastname: Lee
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D031172:Artemisia annua / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006046:Gold / Q000737:chemistry*; D066298:In Vitro Techniques; D053768:Metal Nanoparticles; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors*; D033362:Powder Diffraction; D012834:Silver / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods; D013818:Thermogravimetry
chemical_list: D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D012834:Silver; D006046:Gold; D014442:Monophenol Monooxygenase
keywords: Antibacterial; Antityrosinase; Artemisia annua; Leaf extract; Metal nanoparticles; Reducing agent