title: Characterization of lignocellulolytic activities from a moderate halophile strain of Aspergillus caesiellus isolated from a sugarcane bagasse fermentation.
abstract: A moderate halophile and thermotolerant fungal strain was isolated from a sugarcane bagasse fermentation in the presence of 2 M NaCl that was set in the laboratory. This strain was identified by polyphasic criteria as Aspergillus caesiellus. The fungus showed an optimal growth rate in media containing 1 M NaCl at 28°C and could grow in media added with up to 2 M NaCl. This strain was able to grow at 37 and 42°C, with or without NaCl. A. caesiellus H1 produced cellulases, xylanases, manganese peroxidase (MnP) and esterases. No laccase activity was detected in the conditions we tested. The cellulase activity was thermostable, halostable, and no differential expression of cellulases was observed in media with different salt concentrations. However, differential band patterns for cellulase and xylanase activities were detected in zymograms when the fungus was grown in different lignocellulosic substrates such as wheat straw, maize stover, agave fibres, sugarcane bagasse and sawdust. Optimal temperature and pH were similar to other cellulases previously described. These results support the potential of this fungus to degrade lignocellulosic materials and its possible use in biotechnological applications.
authors.affiliation: Facultad de Ciencias, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico; Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México.
authors.forename: Ramón Alberto
authors.initials: RA
authors.lastname: Batista-García
authors.affiliation: Facultad de Ciencias, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico; Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México.
authors.forename: Edgar
authors.initials: E
authors.lastname: Balcázar-López
authors.affiliation: Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Cuernavaca, Morelos, Mexico.
authors.forename: Estefan
authors.initials: E
authors.lastname: Miranda-Miranda
authors.affiliation: Facultad de Ciencias, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico; Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México.
authors.forename: Ayixón
authors.initials: A
authors.lastname: Sánchez-Reyes
authors.affiliation: Facultad de Ciencias, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico; Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México.
authors.forename: Laura
authors.initials: L
authors.lastname: Cuervo-Soto
authors.affiliation: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México.
authors.forename: Denise
authors.initials: D
authors.lastname: Aceves-Zamudio
authors.affiliation: Facultad de Ciencias Biológicas, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
authors.forename: Karina
authors.initials: K
authors.lastname: Atriztán-Hernández
authors.affiliation: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México.
authors.forename: Catalina
authors.initials: C
authors.lastname: Morales-Herrera
authors.affiliation: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México.
authors.forename: Rocío
authors.initials: R
authors.lastname: Rodríguez-Hernández
authors.affiliation: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México.
authors.forename: Jorge
authors.initials: J
authors.lastname: Folch-Mallol
mesh_terms: D001230:Aspergillus / Q000201:enzymology* / Q000254:growth & development; D002482:Cellulose / Q000737:chemistry*; D005656:Fungal Proteins / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D008031:Lignin / Q000737:chemistry*; D031786:Saccharum / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry
chemical_list: D005656:Fungal Proteins; C036909:lignocellulose; D012965:Sodium Chloride; D002482:Cellulose; D008031:Lignin; C027433:bagasse