title: Human neural crest stem cells derived from human pluripotent stem cells.
abstract: The neural crest cells give rise to neurons and glia in the peripheral nervous system, which is an important component of the nervous system. Here we developed a scalable process of inducing neural crest stem cells (NCSCs) from hESCs/iPSCs by a combination of growth factors in medium conditioned on stromal cells, and showed that NCSCs could be purified by p75 using FACS. In vitro-expanded NCSCs were able to differentiate into neurons and glia (Schwann cells) of the peripheral nervous system (PNS) as well as mesenchymal derivatives.
authors.affiliation: Buck Institute for Research on Aging, 8001 Redwood Blvd., Novato, CA, 94945, USA.
authors.forename: Qiuyue
authors.initials: Q
authors.lastname: Liu
authors.forename: Andrzej
authors.initials: A
authors.lastname: Swistowski
authors.forename: Xianmin
authors.initials: X
authors.lastname: Zeng
mesh_terms: D050156:Adipogenesis; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D053595:Embryonic Stem Cells / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D057026:Induced Pluripotent Stem Cells / Q000166:cytology; D009432:Neural Crest / Q000166:cytology*; D058953:Neural Stem Cells / Q000166:cytology*; D009474:Neurons / Q000166:cytology; D010012:Osteogenesis; D039904:Pluripotent Stem Cells / Q000166:cytology*; D012583:Schwann Cells / Q000166:cytology