title: Two new phenylpropanoids from Micromelum integerrimum.
abstract: OBJECTIVE
  To investigate the chemical and bioactive constituents from the stems and leaves of Micromelum integerrimum.
  
  
  METHODS
  The chemical constituents were isolated and purified by silica gel, Sephadex LH-20, and HPLC. Their structures were mainly elucidated on the basis of extensive 1D- and 2D-NMR spectroscopy and mass spectrometry. Their cytotoxicity and antimicrobial activities were tested by the SRB and turbidimetric methods, respectively.
  
  
  RESULTS
  Two new phenylpropanoids and two known coumarins were obtained, and their structures were identified as microintegerrin A (1), microintegerrin B (2), scopoletin (3), and scopolin (4). All of the compounds were tested for their cytotoxicity against three cancer cell lines (HeLa, A549, and BGC-823) and for antimicrobial activity against the fungus Candida albicans and the bacterium Staphylococcus aureus.
  
  
  CONCLUSIONS
  Two new phenylpropanoids 1 and 2 were isolated and identified from the stems and leaves of M. intgerrimum. None of the compounds showed cytotoxic or antimicrobial activity at the tested concentration of 20 μg·mL(-1).
authors.affiliation: Department of Natural Medicinal Chemistry & State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Zhi-Yao
authors.initials: ZY
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: He
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Wen-Bing
authors.initials: WB
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Guang-Zhi
authors.initials: GZ
authors.lastname: Zeng
authors.affiliation: Department of Natural Medicinal Chemistry & State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: chyzq2005@126.com.
authors.forename: Zhi-Qi
authors.initials: ZQ
authors.lastname: Yin
authors.affiliation: Department of Natural Medicinal Chemistry & State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Shou-Xun
authors.initials: SX
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. Electronic address: nhtan@mail.kib.ac.cn.
authors.forename: Ning-Hua
authors.initials: NH
authors.lastname: Tan
mesh_terms: D002176:Candida albicans / Q000187:drug effects; D003374:Coumarins / Q000302:isolation & purification / Q000494:pharmacology; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D010666:Phenylpropionates / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D018515:Plant Leaves; D018547:Plant Stems; D027821:Rutaceae / Q000737:chemistry*; D012603:Scopoletin / Q000302:isolation & purification / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D003374:Coumarins; D005960:Glucosides; D010666:Phenylpropionates; D010936:Plant Extracts; C000597409:microintegerrin A; C000597410:microintegerrin B; C417572:scopolin; D012603:Scopoletin
keywords: Microintegerrin A; Microintegerrin B; Micromelum integerrimum; Phenylpropanoids; Rutaceae