title: A new phenolic component from Triticum aestivum sprouts and its effects on LPS-stimulated production of nitric oxide and TNF-α in RAW 264.7 cells.
abstract: An unusual new phenolic component, triticumoside (1), and eight known compounds, isoorientin (2), isoscoparin (3), (2R)-2-O-β-D-glucopyranosyloxy-4,7-dimethoxy-2H-1,4-benzoxazin-3(4H)-one (4), adenosine (5), β-sitosterol (6), daucosterol (7), 6′-O-linolenoyl daucosterol (8), α-tocopherol (9), were isolated fromTriticum aestivum sprouts. The hybrid structure of 1, which is a hybrid between a flavone and a polyoxygenated benzene, is rarely found in natural sources. In addition, the effects of these compounds on LPS-induced NO and TNF-α production in RAW 264.7 cells were evaluated. At a concentration of 2.0 μM, compounds 2-4 significantly inhibited the production of both NO and TNF-α. Compound 1 exhibited inhibitory activity on the secretion of TNF-α at concentrations as low as 2.0 μM, but it did not reduce NO levels at any of the tested concentrations.
authors.forename: Bui Thi Thuy
authors.initials: BT
authors.lastname: Luyen
authors.forename: Bui Huu
authors.initials: BH
authors.lastname: Tai
authors.forename: Nguyen Phuong
authors.initials: NP
authors.lastname: Thao
authors.forename: Ji Yun
authors.initials: JY
authors.lastname: Cha
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology*; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D014908:Triticum / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D047309:Flavones; D008070:Lipopolysaccharides; D010636:Phenols; D014409:Tumor Necrosis Factor-alpha; C000592091:triticumoside; D009569:Nitric Oxide