title: Expression of cGMP-dependent protein kinase type I in mature white adipocytes.
abstract: The presence of cGMP-dependent protein kinase I (cGKI) in murine adipocytes has been questioned, although cGKI was implicated in the thermogenic program of fat cells (FCs) and to exert anti-hypertrophic/-inflammatory effects in white adipose tissue. Herein, cGKI was detected in adipocytes from control mice, whereas FCs from global cGKI knockouts (cGKI(-/-)) and cGKIα rescue (αRM) mice remained cGKI-negative. cGKI mutants exhibit decreased adipocyte size, plasma leptin levels and reduced body-weights as compared to litter-matched controls. Low abundance of adiponectin in WAT and plasma of αRM animals together with previously confirmed high IL-6 levels indicate a low-grade inflammation. However, αRMs were protected from streptozotocin-induced hyperglycemia. Our results suggest that cGMP/cGKI affects both glucose and FC homeostasis in more complex mode than previously thought.
authors.affiliation: Department of Pharmacology and Experimental Therapy, Institute of Experimental and Clinical Pharmacology and Toxicology, University Hospital Tuebingen, Tuebingen, Germany; FOR 923, Institute of Pharmacology and Toxicology, Technical University Munich, Muenchen, Germany.
authors.forename: Veronika
authors.initials: V
authors.lastname: Leiss
authors.affiliation: Department of Pharmacology, Toxicology and Clinical Pharmacy, Institute of Pharmacy, University of Tuebingen, Tuebingen, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Illison
authors.affiliation: FOR 923, Institute of Pharmacology and Toxicology, Technical University Munich, Muenchen, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Domes
authors.affiliation: FOR 923, Institute of Pharmacology and Toxicology, Technical University Munich, Muenchen, Germany.
authors.forename: Franz
authors.initials: F
authors.lastname: Hofmann
authors.affiliation: Department of Pharmacology, Toxicology and Clinical Pharmacy, Institute of Pharmacy, University of Tuebingen, Tuebingen, Germany. Electronic address: robert.lukowski@uni-tuebingen.de.
authors.forename: Robert
authors.initials: R
authors.lastname: Lukowski
mesh_terms: D017667:Adipocytes / Q000201:enzymology*; D000818:Animals; D062965:Cyclic GMP-Dependent Protein Kinase Type I / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout
chemical_list: D062965:Cyclic GMP-Dependent Protein Kinase Type I
keywords: Adiponectin; Browning; Interleukin-6; Natriuretic peptides; PKG; cGMP