title: Aedes aegypti salivary protein "aegyptin" co-inoculation modulates dengue virus infection in the vertebrate host.
abstract: Dengue virus (DENV) is transmitted in the saliva of the mosquito vector Aedes aegypti during blood meal acquisition. This saliva is composed of numerous proteins with the capacity to disrupt hemostasis or modulate the vertebrate immune response. One such protein, termed "aegyptin," is an allergen and inhibitor of clot formation, and has been found in decreased abundance in the saliva of DENV-infected mosquitoes. To examine the influence of aegyptin on DENV infection of the vertebrate, we inoculated IRF-3/7(-/- -/-) mice with DENV serotype 2 strain 1232 with and without co-inoculation of aegyptin. Mice that received aegyptin exhibited decreased DENV titers in inoculation sites and in circulation, as well as increased concentrations of GM-CSF, IFN-γ, IL-5, and IL-6, at 48 h post-inoculation when compared to mice that received inoculation of DENV alone. These and other data suggest that aegyptin impacts DENV perpetuation via elevated induction of the immune response.
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Skip Bertman Drive, Baton Rouge, LA 70803, United States.
authors.forename: M K
authors.initials: MK
authors.lastname: McCracken
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Skip Bertman Drive, Baton Rouge, LA 70803, United States.
authors.forename: R C
authors.initials: RC
authors.lastname: Christofferson
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Skip Bertman Drive, Baton Rouge, LA 70803, United States.
authors.forename: B J
authors.initials: BJ
authors.lastname: Grasperge
authors.affiliation: Laboratory of Malaria and Vector Research, National Institutes of Health, 12735 Twinbrook Parkway, Rockville, MD 20852, United States.
authors.forename: E
authors.initials: E
authors.lastname: Calvo
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Skip Bertman Drive, Baton Rouge, LA 70803, United States.
authors.forename: D M
authors.initials: DM
authors.lastname: Chisenhall
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Skip Bertman Drive, Baton Rouge, LA 70803, United States. Electronic address: cmores@lsu.edu.
authors.forename: C N
authors.initials: CN
authors.lastname: Mores
mesh_terms: D000330:Aedes / Q000502:physiology*; D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D003715:Dengue / Q000276:immunology* / Q000821:virology; D003716:Dengue Virus / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D019476:Insect Proteins / Q000276:immunology*; D050838:Interferon Regulatory Factor-3 / Q000235:genetics / Q000378:metabolism; D050839:Interferon Regulatory Factor-7 / Q000235:genetics / Q000378:metabolism; D008297:Male; D018345:Mice, Knockout; D012471:Salivary Proteins and Peptides / Q000276:immunology*
chemical_list: D016207:Cytokines; D019476:Insect Proteins; D050838:Interferon Regulatory Factor-3; D050839:Interferon Regulatory Factor-7; C494233:Irf3 protein, mouse; C494235:Irf7 protein, mouse; D012471:Salivary Proteins and Peptides; C583517:aegyptin protein, Aedes aegypti
keywords: Aegyptin; Allergen; Dengue; Mosquito; Mouse; Saliva; Virus