title: Groundwater geochemistry and microbial community structure in the aquifer transition from volcanic to alluvial areas.
abstract: Groundwaters may act as sinks or sources of organic and inorganic solutes, depending on the relative magnitude of biochemical mobilizing processes and groundwater-surface water exchanges. The objective of this study was to link the lithological and hydrogeological gradients to the aquatic microbial community structure in the transition from aquifer recharge (volcanic formations) to discharge areas (alluvial deposits). A field-scale analysis was performed along a water table aquifer in which volcanic products decreased in thickness and areal extension, while alluvial deposits became increasingly important. We measured the main groundwater physical parameters and the concentrations of major and trace elements. In addition, the microbial community structure was assessed by estimating the occurrence of total coliforms and Escherichia coli, the prokaryotic abundance, the cytometric and phylogenetic community composition. The overall biogeochemical asset differed along the aquifer flow path. The concentration of total and live prokaryotic cells significantly increased in alluvial waters, together with the percentages of Beta- and Delta-Proteobacteria. The microbial propagation over a theoretical groundwater travel time allowed for the identification of microbial groups shifting significantly in the transition between the two different hydrogeochemical facies. The microbial community structure was intimately associated with geochemical changes, thus it should be further considered in view of a better understanding of groundwater ecology and sustainable management strategies.
authors.affiliation: Water Research Institute (IRSA-CNR), Via Salaria Km 29.300, 00015 Monterotondo, Rome, Italy. Electronic address: amalfitano@irsa.cnr.it.
authors.forename: S
authors.initials: S
authors.lastname: Amalfitano
authors.affiliation: Water Research Institute (IRSA-CNR), Via Salaria Km 29.300, 00015 Monterotondo, Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Del Bon
authors.affiliation: Water Research Institute (IRSA-CNR), Via Salaria Km 29.300, 00015 Monterotondo, Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Zoppini
authors.affiliation: Water Research Institute (IRSA-CNR), Via Salaria Km 29.300, 00015 Monterotondo, Rome, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Ghergo
authors.affiliation: Water Research Institute (IRSA-CNR), Via Salaria Km 29.300, 00015 Monterotondo, Rome, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Fazi
authors.affiliation: Water Research Institute (IRSA-CNR), Via Salaria Km 29.300, 00015 Monterotondo, Rome, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Parrone
authors.affiliation: Water Research Institute (IRSA-CNR), Via Salaria Km 29.300, 00015 Monterotondo, Rome, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Casella
authors.affiliation: Water Research Institute (IRSA-CNR), Via Salaria Km 29.300, 00015 Monterotondo, Rome, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Stano
authors.affiliation: Water Research Institute (IRSA-CNR), Via Salaria Km 29.300, 00015 Monterotondo, Rome, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Preziosi
mesh_terms: D001105:Archaea / Q000302:isolation & purification*; D001419:Bacteria / Q000302:isolation & purification*; D004755:Enterobacteriaceae / Q000302:isolation & purification; D019015:Geologic Sediments / Q000737:chemistry; D060587:Groundwater / Q000737:chemistry* / Q000382:microbiology*; D007558:Italy; D014872:Water Movements
keywords: CARD-FISH; Fecal indicator bacteria; Flow cytometry; Hydrogeology; Microbial propagation