title: Light intensity affects chlorophyll synthesis during greening process by metabolite signal from mitochondrial alternative oxidase in Arabidopsis.
abstract: Although mitochondrial alternative oxidase (AOX) has been proposed to play essential roles in high light stress tolerance, the effects of AOX on chlorophyll synthesis are unclear. Previous studies indicated that during greening, chlorophyll accumulation was largely delayed in plants whose mitochondrial cyanide-resistant respiration was inhibited by knocking out nuclear encoded AOX gene. Here, we showed that this delay of chlorophyll accumulation was more significant under high light condition. Inhibition of cyanide-resistant respiration was also accompanied by the increase of plastid NADPH/NADP(+) ratio, especially under high light treatment which subsequently blocked the import of multiple plastidial proteins, such as some components of the photosynthetic electron transport chain, the Calvin-Benson cycle enzymes and malate/oxaloacetate shuttle components. Overexpression of AOX1a rescued the aox1a mutant phenotype, including the chlorophyll accumulation during greening and plastidial protein import. It thus suggests that light intensity affects chlorophyll synthesis during greening process by a metabolic signal, the AOX-derived plastidial NADPH/NADP(+) ratio change. Further, our results thus revealed a molecular mechanism of chloroplast-mitochondria interactions.
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, China.|Department of Genetics, Development, and Cell Biology, Plant Science Institute, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Zhang
authors.affiliation: Institute of Ecological and Environmental Sciences, College of Resources and Environmental Sciences, Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Yuan
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhu
authors.affiliation: College of Biology and Science, Sichuan Agricultural University, Ya'an, 625014, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yuan
authors.affiliation: Department of Genetics, Development, and Cell Biology, Plant Science Institute, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Hua-Xun
authors.initials: HX
authors.lastname: Ye
authors.affiliation: Department of Genetics, Development, and Cell Biology, Plant Science Institute, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Hong-Qing
authors.initials: HQ
authors.lastname: Guo
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Lv
authors.affiliation: Department of Genetics, Development, and Cell Biology, Plant Science Institute, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Yanhai
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, China.
authors.forename: Hong-Hui
authors.initials: HH
authors.lastname: Lin
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D019069:Cell Respiration; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D017930:Genes, Reporter; D008027:Light; D008928:Mitochondria / Q000201:enzymology*; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D008954:Models, Biological; D009249:NADP / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D010788:Photosynthesis; D010836:Phytol / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D045725:Tetrapyrroles / Q000378:metabolism; D000099188:Alternative Oxidase
chemical_list: D002734:Chlorophyll; D024101:Mitochondrial Proteins; D009249:NADP; D010088:Oxidoreductases; D010836:Phytol; D010940:Plant Proteins; D045725:Tetrapyrroles; D000099188:Alternative Oxidase
keywords: NADPH/NADP+ ratio; metabolite signalling; photosynthesis activity