title: A microfluidic nano-biosensor for the detection of pathogenic Salmonella.
abstract: Rapid detection of pathogenic Salmonella in food products is extremely important for protecting the public from salmonellosis. The objective of the present study was to explore the feasibility of using a microfluidic nano-biosensor to rapidly detect pathogenic Salmonella. Quantum dot nanoparticles were used to detect Salmonella cells. For selective detection of Salmonella, anti-Salmonella polyclonal antibodies were covalently immobilized onto the quantum dot surface. To separate and concentrate the cells from the sample, superparamagnetic particles and a microfluidic chip were used. A portable fluorometer was developed to measure the fluorescence signal from the quantum dot nanoparticles attached to Salmonella in the samples. The sensitivity for detection of pathogenic Salmonella was evaluated using serially diluted Salmonella Typhimurium in borate buffer and chicken extract. The fluorescence response of the nano-biosensor increased with increasing cell concentration. The detection limit of the sensor was 10(3) CFU/mL Salmonella in both borate buffer and food extract.
authors.affiliation: National Academy of Agricultural Science, Department of Agricultural Engineering, RDA, 150 Suin-Ro, Kweonseonku, Suwon 441-100, Republic of Korea. Electronic address: giyoung@korea.kr.
authors.forename: Giyoung
authors.initials: G
authors.lastname: Kim
authors.affiliation: National Academy of Agricultural Science, Department of Agricultural Engineering, RDA, 150 Suin-Ro, Kweonseonku, Suwon 441-100, Republic of Korea.
authors.forename: Ji-Hea
authors.initials: JH
authors.lastname: Moon
authors.affiliation: National Academy of Agricultural Science, Department of Agricultural Engineering, RDA, 150 Suin-Ro, Kweonseonku, Suwon 441-100, Republic of Korea.
authors.forename: Chang-Yeon
authors.initials: CY
authors.lastname: Moh
authors.affiliation: National Academy of Agricultural Science, Department of Agricultural Engineering, RDA, 150 Suin-Ro, Kweonseonku, Suwon 441-100, Republic of Korea.
authors.forename: Jong-guk
authors.initials: JG
authors.lastname: Lim
mesh_terms: D058491:Bacterial Load / Q000295:instrumentation; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology / Q000295:instrumentation*; D018189:Immunomagnetic Separation / Q000295:instrumentation; D056656:Lab-On-A-Chip Devices / Q000295:instrumentation*; D015203:Reproducibility of Results; D012475:Salmonella / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation
keywords: Food safety; Microfluidics; Nano-biosensor; Salmonella Typhimurium