title: Light-induced fluctuations in biomass accumulation, secondary metabolites production and antioxidant activity in cell suspension cultures of Artemisia absinthium L.
abstract: Light is an important factor influencing plant morphogenesis and biochemical pathways, including biosynthesis of primary and secondary metabolites. In the present study, we investigated the differential effect of light on biomass accumulation and secondary metabolites production in cell suspension cultures of Artemisia absinthium L. A prolonged log phase of 21 days was followed by light-grown cultures. Light-grown cultures displayed 3.9-fold maximum increase (8.88 g/l) in dry biomass on day 30 of culture which was comparable to 3.7-fold maximum increase (9.2 g/l) on day 27 in dark-grown cultures. Compared to dark grown-cultures, enhanced levels of total phenolic content (5.32 mg/g DW), total phenolic production (42.96 mg/l) and total secondary metabolites (6.79 mg/g) were found in light-grown suspension cultures during the log phase of growth. Further, a positive correlation among maximum levels of antioxidant activity (63.8%), total phenolic production (42.96 mg/l) and total secondary metabolites (6.79 mg/g DW) was displayed by light-grown suspension cultures.
authors.affiliation: Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ali
authors.affiliation: Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan. Electronic address: bhabbasi@qau.edu.pk.
authors.forename: Bilal Haider
authors.initials: BH
authors.lastname: Abbasi
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D031171:Artemisia absinthium / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D018533:Biomass; D018929:Cell Culture Techniques; D005419:Flavonoids / Q000378:metabolism; D008027:Light; D010636:Phenols / Q000378:metabolism; D013997:Time Factors
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols
keywords: Antioxidant; Artemisia; Cell suspension; Flavonoids; Light; Phenolics