title: Assembly of recombinant Israeli Acute Paralysis Virus capsids.
abstract: The dicistrovirus Israeli Acute Paralysis Virus (IAPV) has been implicated in the worldwide decline of honey bees. Studies of IAPV and many other bee viruses in pure culture are restricted by available isolates and permissive cell culture. Here we show that coupling the IAPV major structural precursor protein ORF2 to its cognate 3C-like processing enzyme results in processing of the precursor to the individual structural proteins in a number of insect cell lines following expression by a recombinant baculovirus. The efficiency of expression is influenced by the level of IAPV 3C protein and moderation of its activity is required for optimal expression. The mature IAPV structural proteins assembled into empty capsids that migrated as particles on sucrose velocity gradients and showed typical dicistrovirus like morphology when examined by electron microscopy. Monoclonal antibodies raised to recombinant capsids were configured into a diagnostic test specific for the presence of IAPV. Recombinant capsids for each of the many bee viruses within the picornavirus family may provide virus specific reagents for the on-going investigation of the causes of honeybee loss.
authors.affiliation: School of Biological Sciences, University of Reading, Reading, United Kingdom.
authors.forename: Junyuan
authors.initials: J
authors.lastname: Ren
authors.affiliation: School of Biological Sciences, University of Reading, Reading, United Kingdom.
authors.forename: Abigail
authors.initials: A
authors.lastname: Cone
authors.affiliation: School of Biological Sciences, University of Reading, Reading, United Kingdom.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Willmot
authors.affiliation: School of Biological Sciences, University of Reading, Reading, United Kingdom.
authors.forename: Ian M
authors.initials: IM
authors.lastname: Jones
mesh_terms: D000818:Animals; D001516:Bees / Q000821:virology*; D002213:Capsid / Q000378:metabolism*; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D056631:Colony Collapse / Q000821:virology*; D056629:Dicistroviridae / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D036022:Capsid Proteins; D011994:Recombinant Proteins