title: Extracellular biosynthesis of zinc oxide nanoparticles using Rhodococcus pyridinivorans NT2: multifunctional textile finishing, biosafety evaluation and in vitro drug delivery in colon carcinoma.
abstract: In this study, zinc oxide (ZnO) nanoparticles (NPs) were rapidly synthesized from zinc sulfate solution at room temperature using a metabolically versatile actinobacteria Rhodococcus pyridinivorans NT2. The morphology, structure and stability of the synthesized ZnO NPs were studied using UV-visible absorption spectroscopy, X-ray Diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscopy (FESEM) with energy dispersive spectroscopy (EDS), transmission electron microscopy (TEM), Zeta potential, and thermogravimetry. The data indicated that the synthesized nanoparticles were moderately stable, hexagonal phase, roughly spherical with average particle diameter in the range of 100-120 nm. Results obtained on examination of protein expression revealed that cell enzymes and extracellular protein systems of Rhodococcus sp. may take part in synthesis process. Furthermore, the ZnO NPs were coated onto textile fabrics to enhance UV-blocking, self-cleaning and antibacterial properties. Ultraviolet protecting factor (UPF) indicating UV-blocking properties of ZnO NPs coated textile fabrics were determined as 65, 88, 121, 172 and 241 for 1, 2, 3, 4 and 5 gm(-2) of ZnO NPs, respectively. Besides, self-cleaning activity was assessed by investigating photocatalytic activity on malachite green as well as antibacterial activity against aerobic Gram-positive Staphylococcus epidermidis NCIM 2493 (ATCC 12228). The antibacterial effects of these textiles were evaluated using ISO 20743 standard. In addition, ZnO NPs exhibited a preferential ability to kill HT-29 cancerous cells as compared with normal peripheral blood mononuclear cells (PBMCs).
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.
authors.forename: Debasree
authors.initials: D
authors.lastname: Kundu
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.
authors.forename: Chinmay
authors.initials: C
authors.lastname: Hazra
authors.affiliation: University Institute of Chemical Technology, North Maharashtra University, Jalgaon, Maharashtra, India. Electronic address: aniruddha_chatterjee2006@yahoo.co.in.
authors.forename: Aniruddha
authors.initials: A
authors.lastname: Chatterjee
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.
authors.forename: Ambalal
authors.initials: A
authors.lastname: Chaudhari
authors.affiliation: University Institute of Chemical Technology, North Maharashtra University, Jalgaon, Maharashtra, India.
authors.forename: Satyendra
authors.initials: S
authors.lastname: Mishra
mesh_terms: D000880:Anthraquinones / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D003110:Colonic Neoplasms / Q000473:pathology*; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D065546:Drug Liberation; D005110:Extracellular Space / Q000378:metabolism*; D019073:HT29 Cells; D006801:Humans; D053758:Nanoparticles; D010316:Particle Size; D010782:Photolysis; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D012240:Rhodococcus / Q000166:cytology / Q000378:metabolism*; D012449:Safety; D013212:Staphylococcus epidermidis / Q000187:drug effects; D013696:Temperature; D013784:Textiles; D014466:Ultraviolet Rays; D017155:Wettability; D015034:Zinc Oxide / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D004337:Drug Carriers; D011837:Radiation-Protective Agents; D015034:Zinc Oxide
keywords: Cytotoxicity; HT-29; Nanostructures; Optical properties; Photocatalysis; Staphylococcus epidermidis