title: Modified poly(lactic-co-glycolic acid) nanoparticles for enhanced cellular uptake and gene editing in the lung.
abstract: Surface-modified poly(lactic-co-glycolic acid) (PLGA)/poly(β-aminoester)(PBAE)nanoparticles (NPs) have shown great promise in gene delivery. In this work, the pulmonary cellular uptake of these NPs is evaluated and surface-modified PLGA/PBAE NPs are shown to achieve higher cellular association and gene editing than traditional NPs composed of PLGA or PLGA/PBAE blends alone.
authors.affiliation: Department of Biomedical Engineering, Yale University, New Haven, CT, 06511, USA.
authors.forename: Rachel J
authors.initials: RJ
authors.lastname: Fields
authors.forename: Elias
authors.initials: E
authors.lastname: Quijano
authors.forename: Nicole Ali
authors.initials: NA
authors.lastname: McNeer
authors.forename: Christina
authors.initials: C
authors.lastname: Caputo
authors.forename: Raman
authors.initials: R
authors.lastname: Bahal
authors.forename: Kavi
authors.initials: K
authors.lastname: Anandalingam
authors.forename: Marie E
authors.initials: ME
authors.lastname: Egan
authors.forename: Peter M
authors.initials: PM
authors.lastname: Glazer
authors.forename: W Mark
authors.initials: WM
authors.lastname: Saltzman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004247:DNA / Q000008:administration & dosage; D004268:DNA-Binding Proteins / Q000737:chemistry; D004337:Drug Carriers; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005260:Female; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D019344:Lactic Acid / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D016676:Macrophages, Alveolar / Q000187:drug effects; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000008:administration & dosage / Q000493:pharmacokinetics*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013499:Surface Properties
chemical_list: D004268:DNA-Binding Proteins; D004337:Drug Carriers; C475074:MPG peptide; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004247:DNA
keywords: cell-penetrating peptide; cellular uptake; gene editing; nanoparticles; pulmonary delivery