title: In vitro antioxidant, antibacterial, and cytotoxic activity and in vivo effect of Syngonium podophyllum and Eichhornia crassipes leaf extracts on isoniazid induced oxidative stress and hepatic markers.
abstract: The present study reports the in vitro antioxidant, antibacterial, and cytotoxic potential of Syngonium podophyllum (SP) and Eichhornia crassipes (EC) leaf aqueous extracts as well as their in vivo effect on oxidative stress and hepatic biomarkers in isoniazid induced rats. Phytochemical screening of extracts revealed the presence of flavonoids, terpenoids, reducing sugars, alkaloids, and saponins. Phenolic content in SP and EC extracts was 5.36 ± 0.32 and 10.63 ± 0.13 mg PGE/g, respectively, while flavonoid content was 1.26 ± 0.03 and 0.51 ± 0.03 μg QE/mg, respectively. EC extract exhibited comparatively better antioxidant activity as indicated by reducing power (0.197-0.775), DPPH radical scavenging potential (11%-96%), and metal ion chelating ability (42%-93%). Both the extracts provided 13%-65% protection against lipid peroxidation in rat tissue (liver, kidney, and brain) homogenate. SP and EC extracts exhibited 51% and 43% cytotoxicity against lung cancer (NCI-H322) cell line, respectively. Both extracts demonstrated considerable antibacterial activity against Proteus vulgaris, Salmonella typhi, and Bordetella bronchiseptica. Coadministration of E. crassipes extract with isoniazid in rats accounted for 46% decrease in malondialdehyde content and 21% increase in FRAP value of plasma. It also mitigated the isoniazid induced alterations in serum enzymes (SGOT, SGPT, and ALP), total bilirubin, creatinine, and hemoglobin contents. S. podophyllum extract was found to be hepatotoxic.
authors.affiliation: Department of Biochemistry, University of Allahabad, Allahabad 211002, India.
authors.forename: Shashank
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Biochemistry, University of Allahabad, Allahabad 211002, India.
authors.forename: Ramesh
authors.identifier: 0000-0002-8236-5016
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Department of Biochemistry, University of Allahabad, Allahabad 211002, India.
authors.forename: Astha
authors.identifier: 0000-0002-2995-7530
authors.initials: A
authors.lastname: Dwivedi
authors.affiliation: Department of Biochemistry, University of Allahabad, Allahabad 211002, India.
authors.forename: Abhay K
authors.initials: AK
authors.lastname: Pandey
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D031806:Eichhornia / Q000737:chemistry; D016166:Free Radical Scavengers; D007538:Isoniazid / Q000633:toxicity; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D012475:Salmonella / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D007538:Isoniazid