title: Proteomics analysis of altered cellular metabolism induced by insufficient copper level.
abstract: Insufficient copper level in the mammalian cell culture medium resulted in lactate accumulation while maintaining similar growth and culture viability profiles. Label-free, LC-MS/MS-based shotgun proteomics method was applied to compare the protein expression profiles obtained from the cultures exposed to suboptimal copper level to those provided with sufficient amount of copper. Under copper deficient condition, a substantial reduction of the protein levels of the multiple subunits of Complex IV, also known as cytochrome c oxidase, of the mitochondrial electron transport chain was observed for all three different Chinese Hamster Ovary (CHO) cell lines expressing therapeutic monoclonal antibodies tested. Additional proteins affected by suboptimal copper level included peroxiredoxin (PRDX) and hepatocyte-derived growth factor (HDGF), which were affected during early phase of the fed-batch production, several days prior to initiation of lactate accumulation. In contrast, proteins such as syntenin (SDCBP) and integral membrane 2C (ITM2C) showed altered expression patterns toward the end of culture duration, after lactate divergence had occurred. For all conditions tested, time was the most predominant factor facilitating the direction of global protein expression trend, with substantial number of proteins subjected to time-dependent changes in expression, independent of copper.
authors.affiliation: Product Attribute Sciences, Amgen, Inc. , One Amgen Center Drive, Thousand Oaks, CA 91320, USA. Electronic address: sohyek@amgen.com.
authors.forename: Sohye
authors.initials: S
authors.lastname: Kang
authors.affiliation: Product Attribute Sciences, Amgen, Inc. , One Amgen Center Drive, Thousand Oaks, CA 91320, USA.
authors.forename: Gang
authors.initials: G
authors.lastname: Xiao
authors.affiliation: Product Attribute Sciences, Amgen, Inc. , One Amgen Center Drive, Thousand Oaks, CA 91320, USA.
authors.forename: Da
authors.initials: D
authors.lastname: Ren
authors.affiliation: Product Attribute Sciences, Amgen, Inc. , One Amgen Center Drive, Thousand Oaks, CA 91320, USA.
authors.forename: Zhongqi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Drug Substance Development, Amgen, Inc. , One Amgen Center Drive, Thousand Oaks, CA 91320, USA.
authors.forename: Nicole
authors.initials: N
authors.lastname: Le
authors.affiliation: Drug Substance Development, Amgen, Inc. , 1201 Amgen Court West, Seattle, WA 98119, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Trentalange
authors.affiliation: Drug Substance Development, Amgen, Inc. , One Amgen Center Drive, Thousand Oaks, CA 91320, USA.
authors.forename: Shivani
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Drug Substance Development, Amgen, Inc. , One Amgen Center Drive, Thousand Oaks, CA 91320, USA.
authors.forename: Henry
authors.initials: H
authors.lastname: Lin
authors.affiliation: Product Attribute Sciences, Amgen, Inc. , One Amgen Center Drive, Thousand Oaks, CA 91320, USA.
authors.forename: Pavel V
authors.initials: PV
authors.lastname: Bondarenko
mesh_terms: D000818:Animals; D016466:CHO Cells; D003300:Copper / Q000172:deficiency* / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D003576:Electron Transport Complex IV / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D019344:Lactic Acid; D003300:Copper; D003576:Electron Transport Complex IV
keywords: Chinese hamster ovary cells; Complex IV; Copper; Cytochrome c oxidase; Lactate; Proteomics