title: A standard cytogenetic map for Anopheles sinensis and chromosome arm homology between the subgenera Anopheles and Cellia.
abstract: Anopheles sinensis (Diptera: Culicidae) is an important vector of Plasmodium vivax in Southeast Asia. To facilitate population genetic and genomic studies of An. sinensis, we developed a standard cytogenetic photomap for this species. The polytene chromosomes were straightened and divided into 39 numbered divisions and 116 lettered subdivisions. The chromosomal localizations of 13 DNA probes were determined by fluorescent in situ hybridization. A comparison of the physical map for An. sinensis with the genome map for Anopheles gambiae revealed a whole-arm autosomal translocation between the two species. Specifically, the 2R arm of An. gambiae corresponds to the 3R arm of An. sinensis and the pattern of correspondence of the other chromosome arms remains regular. We mapped the breakpoints of the polymorphic paracentric chromosomal inversion 3Ra to subdivisions 28A and 31A. The standard cytogenetic map developed in this study will be useful for detailed comparative genome mapping and population genetic studies of An. sinensis.
authors.affiliation: Department of Entomology, Nanjing Agricultural University, Nanjing, China.
authors.forename: J
authors.initials: J
authors.lastname: Liang
authors.forename: M V
authors.initials: MV
authors.lastname: Sharakhova
authors.forename: Q
authors.initials: Q
authors.lastname: Lan
authors.forename: H
authors.initials: H
authors.lastname: Zhu
authors.forename: I V
authors.initials: IV
authors.lastname: Sharakhov
authors.forename: A
authors.initials: A
authors.lastname: Xia
mesh_terms: D000818:Animals; D000852:Anopheles / Q000166:cytology / Q000235:genetics*; D007446:Chromosome Inversion; D002874:Chromosome Mapping; D020732:Cytogenetic Analysis; D015342:DNA Probes; D023061:Gene Order; D017404:In Situ Hybridization, Fluorescence; D059007:Polytene Chromosomes / Q000235:genetics*; D012469:Salivary Glands / Q000166:cytology; D014178:Translocation, Genetic
chemical_list: D015342:DNA Probes
keywords: Anopheles sinensis; arm homology; fluorescent in situ hybridization; polymorphic inversion; polytene chromosomes