title: Diagnostic procedures for paraffin-embedded tissues analysis in pharmacogenomic studies.
abstract: In this book chapter we report our own experience of mutational analysis in selecting tailored anticancer treatments for solid tumors. Our Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy, routinely performs pharmacogenetic screenings for different genes such as K-ras, BRAF, KIT, PDGFRα, and EGFR on paraffin-embedded cancer sections. Therefore, the chapter describes the mutational analysis procedures on paraffin-embedded tumors aimed to predict individual response to anticancer therapy. These molecular diagnostic methodologies may help us in improving the translational impact of genetic information on clinical practice.
authors.affiliation: Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy.
authors.forename: Raffaele
authors.initials: R
authors.lastname: Palmirotta
authors.forename: Maria Laura
authors.initials: ML
authors.lastname: De Marchis
authors.forename: Giorgia
authors.initials: G
authors.lastname: Ludovici
authors.forename: Patrizia
authors.initials: P
authors.lastname: Ferroni
authors.forename: Pasquale
authors.initials: P
authors.lastname: Abete
authors.forename: Fiorella
authors.initials: F
authors.lastname: Guadagni
authors.forename: David
authors.initials: D
authors.lastname: Della-Morte
mesh_terms: D004252:DNA Mutational Analysis / Q000379:methods; D016591:Histocytological Preparation Techniques; D006801:Humans; D025202:Molecular Diagnostic Techniques; D009369:Neoplasms / Q000188:drug therapy / Q000235:genetics; D010597:Pharmacogenetics / Q000379:methods*; D057285:Precision Medicine