title: Capsaicin induces high expression of BAFF and aberrantly glycosylated IgA1 of tonsillar mononuclear cells in IgA nephropathy patients.
abstract: BACKGROUND
  IgA nephropathy (IgAN) is the most common type of primary glomerulonephritis in the world. Hot pepper is the most favorite vegetable for Chinese in Hunan and Sichuan provinces. It can be assumed that capsaicin, the active ingredient of hot pepper, is a possible risk factor in diet in the pathogenesis of IgAN.
  
  
  METHODS
  22 subjects, including 11 IgAN patients and 11 non-IgAN patients were enrolled in this study. Tonsillar mononuclear cells were isolated and cultured for 3days with or without capsaicin.
  
  
  RESULTS
  In the absence and presence of capsaicin, the BAFF expression and IgA1 secretion were higher in IgAN patients than that in non-IgAN patients, meanwhile, the gene expression of C1GALT1 and Cosmc and IgA1 O-glycosylation level were significantly lower. In IgAN group, coincubated with capsaicin, IgA1 and BAFF secretion and BAFF expression by TMCs were significantly higher than that without capsaicin, furthermore, the level of mRNA encoding C1GALT1 and Cosmc and the level of IgA1 O-glycosylation were evidently lower.
  
  
  CONCLUSIONS
  Capsaicin may induce IgA1 secretion by activating BAFF expression, and bring to aberrantly IgA1 O-glycosylation by suppressing C1GALT1 and Cosmc expression. Therefore, to limit the consumption of hot pepper would be beneficial to patients with IgA nephropathy.
authors.affiliation: Nephrology Department, 2nd Xiangya Hospital, Central South University, Hunan Key Lab of Kidney Disease and Blood Purification, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shao
authors.affiliation: Nephrology Department, 2nd Xiangya Hospital, Central South University, Hunan Key Lab of Kidney Disease and Blood Purification, People's Republic of China. Electronic address: ympeng2011@aliyun.com.
authors.forename: Youming
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Nephrology Department, 2nd Xiangya Hospital, Central South University, Hunan Key Lab of Kidney Disease and Blood Purification, People's Republic of China.
authors.forename: Liyu
authors.initials: L
authors.lastname: He
authors.affiliation: Nephrology Department, 2nd Xiangya Hospital, Central South University, Hunan Key Lab of Kidney Disease and Blood Purification, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Nephrology Department, 2nd Xiangya Hospital, Central South University, Hunan Key Lab of Kidney Disease and Blood Purification, People's Republic of China.
authors.forename: Xian
authors.initials: X
authors.lastname: Chen
authors.affiliation: Nephrology Department, 2nd Xiangya Hospital, Central South University, Hunan Key Lab of Kidney Disease and Blood Purification, People's Republic of China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Peng
mesh_terms: D000293:Adolescent; D000328:Adult; D053264:B-Cell Activating Factor / Q000235:genetics / Q000378:metabolism*; D002211:Capsaicin / Q000494:pharmacology*; D002212:Capsicum / Q000276:immunology; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D002681:China; D004032:Diet / Q000009:adverse effects; D015536:Down-Regulation; D005260:Female; D005700:Galactosyltransferases / Q000235:genetics / Q000378:metabolism; D005922:Glomerulonephritis, IGA / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D008297:Male; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D050916:TRPV Cation Channels / Q000819:agonists*; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D053264:B-Cell Activating Factor; C505369:C1GALT1C1 protein, human; D007070:Immunoglobulin A; D018832:Molecular Chaperones; C505719:TNFSF13B protein, human; D050916:TRPV Cation Channels; C505293:C1GALT1 protein, human; D005700:Galactosyltransferases; D002211:Capsaicin
keywords: BAFF; C1GALT1; Capsaicin; Cosmc; IgA nephropathy