title: Generation of murine xenograft models of brain tumors from primary human tissue for in vivo analysis of the brain tumor-initiating cell.
abstract: The generation of xenograft models, which support the growth of human tissue in animals, forms an important part of a researcher's tool kit and enhances the ability to understand the initiation and development of cancer in vivo. Especially in the context of the brain tumor-initiating cell (BTIC), a xenograft model allows for careful characterization of BTIC roles in tumor initiation, growth, and relapse. Here, we detail a set of procedures which describe the isolation, enrichment, and intracranial injection of human BTICs from patient samples to generate xenograft models of a human brain tumor.
authors.affiliation: McMaster Children's Hospital, McMaster University, 1280 Main Street West, Hamilton, ON, Canada, L8S 4K1.
authors.forename: Maleeha
authors.initials: M
authors.lastname: Qazi
authors.forename: Aneet
authors.initials: A
authors.lastname: Mann
authors.forename: Randy
authors.initials: R
authors.lastname: van Ommeren
authors.forename: Chitra
authors.initials: C
authors.lastname: Venugopal
authors.forename: Nicole
authors.initials: N
authors.lastname: McFarlane
authors.forename: Parvez
authors.initials: P
authors.lastname: Vora
authors.forename: Sheila K
authors.initials: SK
authors.lastname: Singh
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology*; D002471:Cell Transformation, Neoplastic; D004195:Disease Models, Animal; D005434:Flow Cytometry; D064593:Heterografts; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D061251:Primary Cell Culture; D014407:Tumor Cells, Cultured