title: Investigations into the bovine serum albumin binding and fluorescence properties of Tb (III) complex of a novel 8-hydroxyquinoline ligand.
abstract: A novel ligand, 2-methyl-6-(8-quinolinyl)-dicarboxylate pyridine (L), and its corresponding Tb (III) complex, Na4Tb(L)2Cl4·3H2O, were successfully prepared and characterized. The luminescence spectra showed that the ligand L was an efficient sensitizer for Tb (III) luminescence. The interaction of the complex with bovine serum albumin (BSA) was investigated through fluorescence spectroscopy under physiological conditions. The Stern-Volmer analysis indicated that the fluorescence quenching was resulted from static mechanism. The binding sites (n) approximated 1.0 and this meant that interaction of Na4Tb(L)2Cl4·3H2O with BSA had single binding site. The results showed van der Waals interactions and hydrogen bonds played major roles in the binding reaction. Furthermore, circular dichroism (CD) spectra indicated that the conformation of BSA was changed.
authors.affiliation: School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China; Hunan Police Academy, Changsha 410138, PR China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Zhao
authors.affiliation: School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China. Electronic address: trr@mail.csu.edu.cn.
authors.forename: Ruiren
authors.initials: R
authors.lastname: Tang
authors.affiliation: School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Xu
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002942:Circular Dichroism; D007700:Kinetics; D008024:Ligands; D015125:Oxyquinoline / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013725:Terbium / Q000737:chemistry / Q000378:metabolism*; D013818:Thermogravimetry
chemical_list: D008024:Ligands; D013725:Terbium; D012710:Serum Albumin, Bovine; D015125:Oxyquinoline
keywords: 8-Hydroxyquinoline; BSA binding study; Circular dichroism spectroscopy; Fluorescence property; Tb (III) complex