title: Synthetic lethal screen of NAA20, a catalytic subunit gene of NatB N-terminal acetylase in Saccharomyces cerevisiae.
abstract: The Saccharomyces cerevisiae NatB N-terminal acetylase contains a catalytic subunit Naa20 and an auxiliary subunit Naa25. To elucidate the cellular functions of the NatB, we utilized the Synthetic Genetic Array to screen for genes that are essential for cell growth in the absence of NAA20. The genome-wide synthetic lethal screen of NAA20 identified genes encoding for serine/threonine protein kinase Vps15, 1,3-beta-glucanosyltransferase Gas5, and a catabolic repression regulator Mig3. The present study suggests that the catalytic activity of the NatB N-terminal aceytase is involved in vacuolar protein sorting and cell wall maintenance.
authors.affiliation: Department of Life Sciences, Pohang University of Science and Technology, Gyeongbuk, 790-784, Republic of Korea.
authors.forename: Kang-Eun
authors.initials: KE
authors.lastname: Lee
authors.forename: Jun-Young
authors.initials: JY
authors.lastname: Ahn
authors.forename: Jeong-Mok
authors.initials: JM
authors.lastname: Kim
authors.forename: Cheol-Sang
authors.initials: CS
authors.lastname: Hwang
mesh_terms: D020134:Catalytic Domain / Q000235:genetics; D017353:Gene Deletion; D020043:Genes, Essential; D005800:Genes, Fungal; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D063210:N-Terminal Acetyltransferase B / Q000235:genetics* / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000254:growth & development / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D058571:Vacuolar Sorting Protein VPS15 / Q000235:genetics / Q000378:metabolism
chemical_list: C539386:Mig3 protein, S cerevisiae; C570986:Nat3 protein, S cerevisiae; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D063210:N-Terminal Acetyltransferase B; C553582:GAS5 protein, S cerevisiae; D005964:Glucosyltransferases; C546854:VPS15 protein, S cerevisiae; D058571:Vacuolar Sorting Protein VPS15