title: Extraction optimization, preliminary characterization and immunological activity of polysaccharides from figs.
abstract: Complex enzyme extraction (CEE), purification, characterization of fig polysaccharides (FPs) from dried figs were investigated. Orthogonal experiment was used to optimize the concentration of cellulose, pectinase and papain. Response surface methodology (RSM) was employed to optimize extraction conditions. The optimum extraction conditions were: enzyme concentration of 1.5%, 1.5%, 0.5% (wt%) of pectinase, papain, cellulose, ratio of water to raw material 40.3 mL/g, extraction time 54.1 min, temperature 34.15 °C and pH 3.8. Under these conditions, the experimental yield was 7.98 ± 0.17%. Two homogeneous heteropolysaccharides (FPs-1-1, FPs-2-1) were purified by DEAE-Sepharose and Sephadex G-200 chromatography, which were composed of rhamnose, arabinose, xylose, mannose, glucose and galactose with molecular weight of 1.52 × 10(6) and 4.75 × 10(5)Da, respectively. The bioactivity assay showed that FPs-1-1 and FPs-2-1 could more significantly enhance splenic lymphocyte proliferation, phagocytosis and NO production of macrophages, could be explored as potential immunopotentiating agent for use in functional food or medicine.
authors.affiliation: College of Chemistry, Changchun Normal University, Changchun 130032, China. Electronic address: ruizhanchen@163.com.
authors.forename: Ruizhan
authors.initials: R
authors.lastname: Chen
authors.affiliation: College of Chemistry, Changchun Normal University, Changchun 130032, China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Li
authors.affiliation: Food Safety Institute of Technology, Jilin Province Economic Management Cadre College, Changchun 130012, China.
authors.forename: Shizhe
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Chemistry, Changchun Normal University, Changchun 130032, China.
authors.forename: Chenguang
authors.initials: C
authors.lastname: Jin
authors.affiliation: College of Chemistry, Changchun Normal University, Changchun 130032, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Lu
mesh_terms: D001089:Arabinose / Q000737:chemistry / Q000302:isolation & purification; D005591:Chemical Fractionation; D003911:Dextrans; D030681:Ficus / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry / Q000302:isolation & purification; D008358:Mannose / Q000737:chemistry / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D003911:Dextrans; D011134:Polysaccharides; D014867:Water; C025614:sephadex; D001089:Arabinose; D008358:Mannose; D005690:Galactose
keywords: Complex enzyme extraction; Fig polysaccharides; Immunological activity