title: MIC gene polymorphism and haplotype diversity in Zhuang nationality of Southern China.
abstract: Zhuang ethnic minority is the largest minority group in China. Here, we report for the first time the polymorphisms of MICA and MICB in a healthy Zhuang population of 209 unrelated individuals. Using polymerase chain reaction-sequence specific priming (PCR-SSP) and sequencing-based typing (PCR-SBT), 13 MICA-sequence alleles and 5 MICA-STR alleles, as well as 11 MICB alleles were detected, among which MICA(∗)010, MICA(∗)A5 and MICB(∗)005:02 were the most frequent alleles. Linkage disequilibria was investigated and the most common two-locus haplotypes were MICB(∗)005:02-MICA(∗)010 and MICB(∗)014-MICA(∗)045. These results suggest informative genetic markers for investigating origins and evolution of MHC class I region haplotypes in Zhuang population.
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Central South University, 110 Xiangya Road, Changsha 410078, China.
authors.forename: E
authors.initials: E
authors.lastname: Chen
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Central South University, 110 Xiangya Road, Changsha 410078, China.
authors.forename: L
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Central South University, 110 Xiangya Road, Changsha 410078, China.
authors.forename: C J
authors.initials: CJ
authors.lastname: Chen
authors.affiliation: Department of Microbiology, Changzhi Medical College, Changzhi 046000, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Central South University, 110 Xiangya Road, Changsha 410078, China.
authors.forename: Q Z
authors.initials: QZ
authors.lastname: Luo
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Central South University, 110 Xiangya Road, Changsha 410078, China. Electronic address: yuping@mail.csu.edu.cn.
authors.forename: P
authors.initials: P
authors.lastname: Yu
mesh_terms: D000328:Adult; D000483:Alleles; D002681:China; D017931:DNA Primers; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D005843:Geography; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D017931:DNA Primers; D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A; C098080:MICB antigen
keywords: Gene polymorphism; Haplotype diversity; MIC; Zhuang nationality