title: Five new triterpenoidal saponins from the roots of Ilex cornuta and their protective effects against H₂O₂-induced cardiomyocytes injury.
abstract: Five new ursane-type triterpenoidal saponins (1-5), together with five known ones (6-10), were isolated from the EtOH extract of the roots of Ilex cornuta. The structures of saponins 1-5 were elucidated as 19α-hydroxyurs-12-en-28-oic acid 3β-O-β-D-glucuronopyranoside (1), 19α-hydroxyurs-12-en-28-oic acid 3β-O-β-D-glucuronopyranoside-6-O-ethyl ester (2), 19α-hydroxyurs-12-en-28-oic acid 3β-O-α-L-arabinopyranosyl-(1→2)-β-D-glucuronopyranoside (3), 3β-O-[α-L-arabinopyranosyl-(1→2)-β-D-glucuronopyranosyl]-19α-hydroxyurs-12-en-28-oic acid 28-O-β-D-glucopyranosyl ester (4) and 3β-O-[α-L-arabinopyranosyl-(1→2)-β-D-glucuronopyranoside-6-O-methyl ester]-19α-hydroxyurs-12-en-28-oic acid 28-O-β-D-glucopyranosyl ester (5), on the basis of spectroscopic analyses (IR, ESI-MS, HR-ESI-MS, 1D and 2D NMR) and chemical reactions. Protective effects of compounds 1-10 against H₂O₂-induced H9c2 cardiomyocyte injury were tested. Compounds 1-5, 7, and 10 showed cell-protective effects. Among them compound 5 exhibited the highest activity. No significant DPPH radical scavenging activity was observed for compounds 1-10.
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou, 215123, China.
authors.forename: Wenlian
authors.initials: W
authors.lastname: Wang
authors.affiliation: National Center for Natural Products Research, and Department of Pharmacognosy, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, MS 38677, United States.
authors.forename: Jianping
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou, 215123, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou, 215123, China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Lu
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou, 215123, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou, 215123, China. Electronic address: xuqiongming@suda.edu.cn.
authors.forename: Qiongming
authors.initials: Q
authors.lastname: Xu
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou, 215123, China.
authors.forename: Xiaoran
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou, 215123, China; National Center for Natural Products Research, and Department of Pharmacognosy, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, MS 38677, United States.
authors.forename: Ikhlas A
authors.initials: IA
authors.lastname: Khan
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou, 215123, China.
authors.forename: Shilin
authors.initials: S
authors.lastname: Yang
mesh_terms: D000818:Animals; D002460:Cell Line; D006861:Hydrogen Peroxide / Q000009:adverse effects; D030017:Ilex / Q000737:chemistry*; D015394:Molecular Structure; D032383:Myocytes, Cardiac / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D012503:Saponins; D014315:Triterpenes; D006861:Hydrogen Peroxide
keywords: Aquifoliaceae; Cardiomyocytes oxidative injury; DPPH radical scavenging activity; Ilex cornuta; Structure identification; Triterpenoid saponins