title: Development of a simple method for the rapid identification of organisms causing anthrax by coagglutination test.
abstract: A protective antigen (PA) based coagglutination test was optimized in the present study for the specific and sensitive identification of bacteria causing anthrax in a cost effective and less risky manner. The test showed 100% specificity and sensitivity up to 9 × 10(3) formalinized vegetative cells or 11 ng of PA. The optimized test also detected anthrax toxin directly from the serum as well as blood of anthrax infected animals indicating the potential application for direct diagnosis of anthrax under field conditions.
authors.affiliation: Division of Bacteriology & Mycology, Indian Veterinary Research Institute, Izatnagar, U.P. 243122, India. Electronic address: sumithravet@gmail.com.
authors.forename: T G
authors.initials: TG
authors.lastname: Sumithra
authors.affiliation: Principal Scientist & Head, Division of Biological Products, Indian Veterinary Research Institute, Izatnagar, U.P. 243122, India. Electronic address: vkchaturvedi@mail.com.
authors.forename: V K
authors.initials: VK
authors.lastname: Chaturvedi
authors.affiliation: Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, U.P. 243122, India. Electronic address: praveen@ivri.res.in.
authors.forename: P K
authors.initials: PK
authors.lastname: Gupta
authors.affiliation: Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, U.P. 243122, India. Electronic address: drsiju291@gmail.com.
authors.forename: S J
authors.initials: SJ
authors.lastname: Siju
authors.affiliation: Division of Pathology, Indian Veterinary Research Institute, Izatnagar, U.P. 243122, India. Electronic address: cpasusan18@gmail.com.
authors.forename: C
authors.initials: C
authors.lastname: Susan
authors.affiliation: Division of Bacteriology & Mycology, Indian Veterinary Research Institute, Izatnagar, U.P. 243122, India. Electronic address: dr.binzy@gmail.com.
authors.forename: J
authors.initials: J
authors.lastname: Bincy
authors.affiliation: Division of Bacteriology & Mycology, Indian Veterinary Research Institute, Izatnagar, U.P. 243122, India. Electronic address: laxmi.upadhyay@hotmail.com.
authors.forename: U
authors.initials: U
authors.lastname: Laxmi
authors.affiliation: Division of Bacteriology & Mycology, Indian Veterinary Research Institute, Izatnagar, U.P. 243122, India. Electronic address: sunitavet@gmail.com.
authors.forename: S C
authors.initials: SC
authors.lastname: Sunita
authors.affiliation: Division of Bacteriology & Mycology, Indian Veterinary Research Institute, Izatnagar, U.P. 243122, India. Electronic address: ajay500rayss@gmail.com.
authors.forename: A K
authors.initials: AK
authors.lastname: Rai
mesh_terms: D000818:Animals; D000881:Anthrax / Q000175:diagnosis*; D000942:Antigens, Bacterial / Q000737:chemistry*; D001408:Bacillus anthracis / Q000302:isolation & purification*; D001427:Bacterial Toxins / Q000737:chemistry*; D001777:Blood Coagulation; D015153:Blotting, Western; D006168:Guinea Pigs; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013234:Stem Cells
chemical_list: D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D011994:Recombinant Proteins; C030325:anthrax toxin
keywords: Anthrax; Coagglutination test; Diagnosis; Protective antigen