title: An electrochemical DNA biosensor for the detection of Mycobacterium tuberculosis, based on signal amplification of graphene and a gold nanoparticle-polyaniline nanocomposite.
abstract: Due to its low growth rate and its fastidious nature, Mycobacterium tuberculosis is difficult to identify. Its rapid and sensitive detection is, however, critical for the control of tuberculosis. Molecular biology, and more recently electrochemical technology, have been exploited for the detection of this pathogen. In the present study, a novel DNA biosensor was developed for the highly sensitive detection of the specific DNA insertion sequence IS6110 of M. tuberculosis, using reduced graphene oxide-gold nanoparticles (rGO-AuNPs) as a sensing platform and gold nanoparticles-polyaniline (Au-PANI) as a tracer label for amplification. Reduced graphene oxide, which has a large surface area, provided a biocompatible matrix. Gold nanoparticles were electrodeposited on the surface of the rGO modified electrode, which not only increased immobilisation of the capture probe but also promoted electronic transfer. The Au-PANI nanocomposite exhibited good biocompatibility and excellent electrochemical activity. It was therefore used as a tracer label for electrochemical detection, which provided a simple preparation process for a signal-on DNA biosensor. With the excellent electroactivity of the Au-PANI nanocomposite, the resulting DNA biosensor exhibited high sensitivity for the detection of M. tuberculosis over a broad linear range, between 1.0 × 10(-15) and 1.0 × 10(-9) M. The DNA biosensor showed good stability and high specificity and provides a new strategy for clinical M. tuberculosis diagnostics and probably also for pathogenic bacteria in general.
authors.affiliation: Department of Clinical Laboratory, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P. R. China. xqyjyk@foxmail.com.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.forename: Dongneng
authors.initials: D
authors.lastname: Jiang
authors.forename: Guiming
authors.initials: G
authors.lastname: Xiang
authors.forename: Linlin
authors.initials: L
authors.lastname: Liu
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Pu
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques; D004269:DNA, Bacterial / Q000032:analysis*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000814:Aniline Compounds; D004269:DNA, Bacterial; C416807:polyaniline; D006046:Gold; D006108:Graphite