title: Evaluation of Shiga toxin 2e-specific chicken egg yolk immunoglobulin: production and neutralization activity.
abstract: Chicken egg yolk immunoglobulin (IgY) against Shiga toxin 2e (Stx2e), a major cause of swine edema disease, was prepared to evaluate its possible clinical applications. The titer of Stx2e-specific IgY in egg yolk derived from three chickens that had been immunized with an Stx2e toxoid increased 2 weeks after primary immunization and remained high until 90 days after this immunization. Anti-Stx2e IgY was found to neutralize the toxicity of Stx2e by reacting with its A and B subunits, indicating that IgY is a cost-effective agent to develop for prophylactic foods or diagnosis kits for edema disease.
authors.affiliation: Department of Microbiology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Arimitsu
authors.forename: Keiko
authors.initials: K
authors.lastname: Sasaki
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kohda
authors.forename: Toshiyasu
authors.initials: T
authors.lastname: Shimizu
authors.forename: Takao
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000032:analysis; D000992:Antitoxins / Q000032:analysis; D002645:Chickens; D004488:Edema Disease of Swine / Q000517:prevention & control; D004530:Egg Yolk / Q000737:chemistry* / Q000276:immunology*; D007136:Immunoglobulins / Q000032:analysis*; D022641:Shiga Toxin 2 / Q000276:immunology*; D013552:Swine
chemical_list: D057134:Antibodies, Neutralizing; D000992:Antitoxins; C016512:IgY; D007136:Immunoglobulins; D022641:Shiga Toxin 2
keywords: Shiga toxin 2e; chicken IgY; edema disease