title: Red-shifted fluorescent aminated derivatives of a conformationally locked GFP chromophore.
abstract: A novel class of fluorescent dyes based on conformationally locked GFP chromophore is reported. These dyes are characterized by red-shifted spectra, high fluorescence quantum yields and pH-independence in physiological pH range. The intra- and intermolecular mechanisms of radiationless deactivation of ABDI-BF2 fluorophore by selective structural locking of various conformational degrees of freedom were studied. A unique combination of solvatochromic and lipophilic properties together with "infinite" photostability (due to a dynamic exchange between free and bound dye) makes some of the novel dyes promising bioinspired tools for labeling cellular membranes, lipid drops and other organelles.
authors.affiliation: Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow (Russia). baranovmikes@gmail.com.
authors.forename: Mikhail S
authors.initials: MS
authors.lastname: Baranov
authors.forename: Kyril M
authors.initials: KM
authors.lastname: Solntsev
authors.forename: Nadezhda S
authors.initials: NS
authors.lastname: Baleeva
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Mishin
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Lukyanov
authors.forename: Konstantin A
authors.initials: KA
authors.lastname: Lukyanov
authors.forename: Ilia V
authors.initials: IV
authors.lastname: Yampolsky
mesh_terms: D000586:Amination; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018613:Microscopy, Confocal; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D011993:Recombinant Fusion Proteins; D012997:Solvents; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: GFP chromophore; fluorescence microscopy; fluorescent labeling; fluorescent probes; solvatochromism