title: PNA-encoded synthesis (PES) of a 10 000-member hetero-glycoconjugate library and microarray analysis of diverse lectins.
abstract: Identification of selective and synthetically tractable ligands to glycan-binding proteins is important in glycoscience. Carbohydrate arrays have had a tremendous impact on profiling glycan-binding proteins and as analytical tools. We report a highly miniaturized synthetic format to access nucleic-acid-encoded hetero-glycoconjugate libraries with an unprecedented diversity in the combinations of glycans, linkers, and capping groups. Novel information about plant and bacterial lectin specificity was obtained by microarray profiling, and we show that a ligand identified on the array can be converted to a high-affinity soluble ligand by straightforward chemistry.
authors.affiliation: Department of Organic Chemistry, University of Geneva, Quai Ernest Ansermet 30, 1211 Geneva 4 (Switzerland).
authors.forename: Alexandre
authors.initials: A
authors.lastname: Novoa
authors.forename: Takuya
authors.initials: T
authors.lastname: Machida
authors.forename: Sofia
authors.initials: S
authors.lastname: Barluenga
authors.forename: Anne
authors.initials: A
authors.lastname: Imberty
authors.forename: Nicolas
authors.initials: N
authors.lastname: Winssinger
mesh_terms: D002240:Carbohydrate Sequence; D015723:Gene Library; D006001:Glycoconjugates / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D008969:Molecular Sequence Data; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D011134:Polysaccharides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism
chemical_list: D006001:Glycoconjugates; D037102:Lectins; D020135:Peptide Nucleic Acids; D011134:Polysaccharides; D011506:Proteins
keywords: DNA display; PNA; glycan array; glycopeptides; microarrays; multivalency