title: Ameloblastin as a putative marker of specific bone compartments.
abstract: Ameloblastin (AMBN), a member of the enamel matrix protein family, has been recently identified as integral part of the skeleton beyond the enamel. However, the specific role of endogenous AMBN in bone tissue is not fully elucidated. This study aims at investigating mRNA expression of AMBN in wild-type mice in different bone sites from early embryonic to adult stages. AMBN mRNA expression started at pre-dental stages in mouse embryos (E10.5) in both head and body parts. Using laser capture microdissection on 3-day-old mice, we showed an unambiguous mRNA expression of AMBN in extra-dental tissue (mandible bone). Screening of AMBN mRNA expression in adult mice (15-week-old) revealed that mRNA expression of AMBN varied according to the bone site; a higher mRNA levels in mandibular and frontal bone compartments were observed when compared to tibia and occipital bones. These results strongly suggest that AMBN expression may be regulated in a site-specific manner and identify AMBN as a putative in vivo marker of the site-specific fingerprint of bone organs.
authors.affiliation: Laboratory of Molecular Oral Pathophysiology, INSERM UMRS 1138, Team Berdal, Cordeliers Research Center, Pierre and Marie Curie University - Paris 6, Paris Descartes University - Paris 5 , Paris , France .
authors.forename: Jaime
authors.initials: J
authors.lastname: Jacques
authors.forename: Dominique
authors.initials: D
authors.lastname: Hotton
authors.forename: Audrey
authors.initials: A
authors.lastname: Asselin
authors.forename: Muriel
authors.initials: M
authors.lastname: De la Dure-Molla
authors.forename: Amélie E
authors.initials: AE
authors.lastname: Coudert
authors.forename: Juliane
authors.initials: J
authors.lastname: Isaac
authors.forename: Ariane
authors.initials: A
authors.lastname: Berdal
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000032:analysis; D001842:Bone and Bones / Q000166:cytology / Q000378:metabolism*; D049109:Cell Proliferation / Q000502:physiology*; D002478:Cells, Cultured; D003746:Dental Enamel Proteins / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D051379:Mice; D010012:Osteogenesis / Q000502:physiology*
chemical_list: C490890:Ambn protein, mouse; D015415:Biomarkers; D003746:Dental Enamel Proteins; C109069:enamel matrix proteins
keywords: Ameloblastin; bone; enamel matrix proteins; endogenous; osteoblast