title: Transcriptional regulation of amelotin gene by proinflammatory cytokines in gingival fibroblasts.
abstract: Amelotin (AMTN) is a secreted protein expressed during the late stages of enamel formation and in the junctional epithelium. Among many differentially expressed genes, we found significantly increased AMTN mRNA level in inflamed gingiva by DNA microarray. The inductions of AMTN mRNA expressions in inflamed gingiva and human gingival fibroblasts (HGF) were confirmed by real-time polymerase chain reaction. To determine the molecular basis of the expression of AMTN and its regulation by proinflammatory cytokines, we have isolated and characterized the promoter region of mouse AMTN gene. Transient transfection assays were performed using luciferase constructs including mouse AMTN gene promoter. Interleukin-1β, Interleukin-6 and tumor necrosis factor-α induced AMTN mRNA levels in HGF. These cytokines increased the luciferase activities of the AMTN promoter constructs in HGF. The results suggest that proinflammatory cytokines induce AMTN gene transcription and a role for AMTN in gingival inflammation.
authors.affiliation: Department of Periodontology, Nihon University School of Dentistry at Matsudo , Chiba , Japan .
authors.forename: Yohei
authors.initials: Y
authors.lastname: Nakayama
authors.forename: Hideki
authors.initials: H
authors.lastname: Takai
authors.forename: Sari
authors.initials: S
authors.lastname: Matsui
authors.forename: Liming
authors.initials: L
authors.lastname: Zhou
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Abiko
authors.forename: Bernhard
authors.initials: B
authors.lastname: Ganss
authors.forename: Yorimasa
authors.initials: Y
authors.lastname: Ogata
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D003746:Dental Enamel Proteins / Q000235:genetics*; D005347:Fibroblasts / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology; D005881:Gingiva / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000235:genetics; D051379:Mice; D014158:Transcription, Genetic
chemical_list: C507067:AMTN protein, human; C507066:Amtn protein, mouse; D016207:Cytokines; D003746:Dental Enamel Proteins
keywords: Amelotin; cytokines; gingival fibroblast; inflammation; periodontitis