title: A functional polymorphism affecting the APOA5 gene expression is causally associated with plasma triglyceride levels conferring coronary atherosclerosis risk in Han Chinese Population.
abstract: Apolipoprotein A5 (APOA5) gene plays a key role in plasma triglyceride (TG) metabolism, and shows the involvement in coronary artery disease (CAD). A set of single nucleotide polymorphisms around the APOA5 gene was identified to be associated with plasma TG levels. It is of biological and clinical importance to discern the genuine genetic determinants. A polymorphism in 3' untranslated region of the APOA5 gene, rs2266788, is deserving of investigation for suggestive clues from the association in multiple independent studies. In this study, rs2266788 was genotyped in 3222 unrelated subjects consisting of 2062 CAD cases and 1160 controls. The statistical analyses indicated that the minor C allele of rs2266788 was significantly associated with elevated plasma TG levels and higher CAD risk. In normal human liver tissues, comparison of global APOA5 mRNA levels among genotypes and allelic expression imbalance analysis showed the decreased gene expression for the C allele. Luciferase assays confirmed a concordant result that transcriptional activity was lowered for the C allele compared with the T allele in four cell lines. Multiple lines of evidence in our study supported that rs2266788 was causally associated with plasma TG levels conferring CAD risk in Han Chinese population owing to a cis-acting effect to the APOA5 gene expression.
authors.affiliation: Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai-MOST Key Laboratory of Health and Disease Genomics, Department of Genetics, Chinese National Human Genome Center, Shanghai, China. Electronic address: shouwh@chgc.sh.cn.
authors.forename: Weihua
authors.initials: W
authors.lastname: Shou
authors.affiliation: Shanghai-MOST Key Laboratory of Health and Disease Genomics, Department of Genetics, Chinese National Human Genome Center, Shanghai, China. Electronic address: wangying@geneskies.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai-MOST Key Laboratory of Health and Disease Genomics, Department of Genetics, Chinese National Human Genome Center, Shanghai, China. Electronic address: pepper.xief@gmail.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Xie
authors.affiliation: Shanghai-MOST Key Laboratory of Health and Disease Genomics, Department of Genetics, Chinese National Human Genome Center, Shanghai, China. Electronic address: wangbl@chgc.sh.cn.
authors.forename: Beilan
authors.initials: B
authors.lastname: Wang
authors.affiliation: Shanghai-MOST Key Laboratory of Health and Disease Genomics, Department of Genetics, Chinese National Human Genome Center, Shanghai, China. Electronic address: yangl@chgc.sh.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Cardiology, Changhai Hospital, The Second Military Medical University, Shanghai, China. Electronic address: doctorwh666@126.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Wu
authors.affiliation: Shanghai-MOST Key Laboratory of Health and Disease Genomics, Department of Genetics, Chinese National Human Genome Center, Shanghai, China. Electronic address: wangyi@chgc.sh.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shanghai-MOST Key Laboratory of Health and Disease Genomics, Department of Genetics, Chinese National Human Genome Center, Shanghai, China. Electronic address: wangzhm@chgc.sh.cn.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Shanghai-MOST Key Laboratory of Health and Disease Genomics, Department of Genetics, Chinese National Human Genome Center, Shanghai, China. Electronic address: shijx@chgc.sh.cn.
authors.forename: Jinxiu
authors.initials: J
authors.lastname: Shi
authors.affiliation: Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai-MOST Key Laboratory of Health and Disease Genomics, Department of Genetics, Chinese National Human Genome Center, Shanghai, China. Electronic address: weihuangsh@gmail.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
keywords: APOA5 gene; Coronary artery disease; Gene expression; Single nucleotide polymorphism; Triglyceride