title: Protection against influenza H7N9 virus challenge with a recombinant NP-M1-HSP60 protein vaccine construct in BALB/c mice.
abstract: A novel influenza virus of H7N9 subtype circulated throughout China in 2013. The high fatality rate, appearance of several family clusters, and transmission in animal models observed during this outbreak accelerated efforts to identify effective strategies to prevent the spread of this influenza subtype. In this study, the recombinant protein NP-M1-HSP60, a fusion of the nucleoprotein and M1 matrix protein of the A/PR/8/34 (H1N1) influenza virus strain and HSP60, was effectively expressed in Escherichia coli and purified as a candidate component for an influenza vaccine. Intranasal immunization of female BALB/c mice with NP-M1-HSP60 in combination with an oil-in-water adjuvant twice at a 2-week interval induced robust humoral, mucosal, and cell-mediated immune responses. Moreover, this immunization strategy completely protected mice from lethal influenza H7N9 virus challenge and significantly inhibited viral replication in the challenged mouse lung. These data suggest that this vaccine construct has great potential for the basic development of an influenza H7N9 vaccine.
authors.affiliation: Beijing 302 Hospital, Beijing 100039, China; Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
authors.forename: Penghui
authors.initials: P
authors.lastname: Yang
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China; Department of Clinical Laboratory, Jiujiang University Hospital, Jiujiang, Jiangxi 332000, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Wang
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
authors.forename: Hongjing
authors.initials: H
authors.lastname: Gu
authors.affiliation: Beijing 302 Hospital, Beijing 100039, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Li
authors.affiliation: Beijing 302 Hospital, Beijing 100039, China.
authors.forename: Keming
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Beijing 302 Hospital, Beijing 100039, China.
authors.forename: Zhouhai
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Beijing 302 Hospital, Beijing 100039, China.
authors.forename: Ruisheng
authors.initials: R
authors.lastname: Li
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
authors.forename: Yueqiang
authors.initials: Y
authors.lastname: Duan
authors.affiliation: Beijing 302 Hospital, Beijing 100039, China. Electronic address: zhangsg302@hotmail.com.
authors.forename: Shaogeng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China. Electronic address: xiliangw@126.com.
authors.forename: Xiliang
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D018834:Chaperonin 60 / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D005260:Female; D006801:Humans; D064766:Influenza A Virus, H7N9 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D007251:Influenza, Human / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D019590:Nucleocapsid Proteins; D016601:RNA-Binding Proteins / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D014611:Vaccination; D014758:Viral Core Proteins / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D014763:Viral Matrix Proteins / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D018834:Chaperonin 60; D007252:Influenza Vaccines; C488936:M1 protein, Influenza A virus; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins; D014763:Viral Matrix Proteins
keywords: H7N9 vaccine; HSP60; Influenza A virus; M1 protein; Nucleoprotein