title: Immobilization of murine anti-BMP-2 monoclonal antibody on various biomaterials for bone tissue engineering.
abstract: Biomaterials are widely used as scaffolds for tissue engineering. We have developed a strategy for bone tissue engineering that entails application of immobilized anti-BMP-2 monoclonal antibodies (mAbs) to capture endogenous BMPs in vivo and promote antibody-mediated osseous regeneration (AMOR). The purpose of the current study was to compare the efficacy of immobilization of a specific murine anti-BMP-2 mAb on three different types of biomaterials and to evaluate their suitability as scaffolds for AMOR. Anti-BMP-2 mAb or isotype control mAb was immobilized on titanium (Ti) microbeads, alginate hydrogel, and ACS. The treated biomaterials were surgically implanted in rat critical-sized calvarial defects. After 8 weeks, de novo bone formation was assessed using micro-CT and histomorphometric analyses. Results showed de novo bone regeneration with all three scaffolds with immobilized anti-BMP-2 mAb, but not isotype control mAb. Ti microbeads showed the highest volume of bone regeneration, followed by ACS. Alginate showed the lowest volume of bone. Localization of BMP-2, -4, and -7 antigens was detected on all 3 scaffolds with immobilized anti-BMP-2 mAb implanted in calvarial defects. Altogether, these data suggested a potential mechanism for bone regeneration through entrapment of endogenous BMP-2, -4, and -7 proteins leading to bone formation using different types of scaffolds via AMOR.
authors.affiliation: Laboratory of Immune Regulation and Tissue Engineering, Herman Ostrow School of Dentistry, University of Southern California, Los Angeles, CA, USA.
authors.forename: Sahar
authors.identifier: 0000-0001-7965-0927
authors.initials: S
authors.lastname: Ansari
authors.affiliation: Department of Applied Oral Sciences, The Forsyth Institute, Cambridge, USA ; Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, Boston, MA, USA.
authors.forename: Marcelo O
authors.initials: MO
authors.lastname: Freire
authors.affiliation: Department of Periodontology, Graduate School of Medicine, Ewha Womans University, Seoul, Republic of Korea.
authors.forename: Eun-Kyoung
authors.initials: EK
authors.lastname: Pang
authors.affiliation: Dental Research Center (DRC) and Tissue Engineering Unit (TERU), Qassim College of Dentistry, Qassim University, Saudi Arabia.
authors.forename: Alaa I
authors.initials: AI
authors.lastname: Abdelhamid
authors.affiliation: Dental Research Center (DRC) and Tissue Engineering Unit (TERU), Qassim College of Dentistry, Qassim University, Saudi Arabia.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Almohaimeed
authors.affiliation: Laboratory of Immune Regulation and Tissue Engineering, Herman Ostrow School of Dentistry, University of Southern California, Los Angeles, CA, USA ; Laboratory of Immune Regulation and Tissue Engineering (LITE), Division of Periodontology, Diagnostic Sciences & Dental Hygiene, Ostrow School of Dentistry, University of Southern California, 925 34th Street, Room 4278, Los Angeles, CA 90089-0641, USA.
authors.forename: Homayoun H
authors.initials: HH
authors.lastname: Zadeh
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry*; D001861:Bone Regeneration / Q000502:physiology; D001842:Bone and Bones / Q000502:physiology*; D005260:Female; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D008863:Microspheres; D010012:Osteogenesis / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate