title: Isolation of two new prenylated flavonoids from Sinopodophyllum emodi fruit by silica gel column and high-speed counter-current chromatography.
abstract: Two new prenylated flavonoids, sinoflavonoids A-B, were isolated from the dried fruits of Sinopodophyllum emodi by silica gel column chromatography (SGCC) and high-speed counter-current chromatography (HSCCC). The 95% ethanol extract was partitioned with petroleum ether, dichloromethane, ethyl acetate, and n-butanol in water, respectively. The ethyl acetate fraction was pre-separated by SGCC with a petroleum ether-acetone gradient. The eluates containing target compounds were further separated by HSCCC with n-hexane-ethyl acetate-methanol-water (4:6:4:4, v/v). Finally, 17.3mg of sinoflavonoid A and 25.9mg of sinoflavonoid B were obtained from 100mg of the pretreated concentrate. The purities of sinoflavonoid A and sinoflavonoid B were 98.47% and 99.38%, respectively, as determined by HPLC. Their structures were elucidated on the basis of spectroscopic evidences (HR-ESI-MS, (1)H-NMR, (13)C-NMR, HSQC, HMBC). The separation procedures proved to be efficient, especially for trace prenylated flavonoids.
authors.affiliation: Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450046, China; School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450046, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Agronomy, Shandong Agricultural University, Taian, Shandong 271018, China.
authors.forename: Yinshi
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450046, China; School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450046, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450046, China; School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450046, China.
authors.forename: Zhiyou
authors.initials: Z
authors.lastname: Hao
authors.affiliation: Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450046, China; School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450046, China.
authors.forename: Junmin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450046, China; School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450046, China.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Guan
authors.affiliation: Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450046, China; School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450046, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450046, China; School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450046, China. Electronic address: fwsh@hactcm.edu.cn.
authors.forename: Weisheng
authors.initials: W
authors.lastname: Feng
authors.affiliation: Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450046, China; School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450046, China. Electronic address: zhengxk.2006@163.com.
authors.forename: Xiaoke
authors.initials: X
authors.lastname: Zheng
mesh_terms: D028141:Berberidaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods*; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification*; D005638:Fruit / Q000737:chemistry*
chemical_list: D005419:Flavonoids
keywords: High-speed counter-current chromatography; New prenylated flavonoid; Silica gel column chromatography; Sinopodophyllum emodi