title: Liquid chromatography-mass spectrometry based serum peptidomic approach for renal clear cell carcinoma diagnosis.
abstract: Serum peptidomic approach was applied to investigate the peptidomic signature and discover the clinical biomarkers and biomarker patterns for RCC patients. The holistic orthogonal partial least-squares-discriminant analysis (OPLS-DA) based on qualified profile data successfully classified RCC patients from healthy controls, showing 100% sensitivity and specificity. Following critical criteria, several peptides presenting significant differences in serum level were picked out. The unsupervised hierarchical cluster analysis on those peptides was performed, showing 100% sensitivity and 93.3% specificity for RCC diagnosis regarding the present samples. Besides, receiver-operating characteristic (ROC) analysis was applied on single peptide biomarkers, with four peptides showing excellent predictive power. Among them, IYQLNSKLV and AGISMRSGDSPQD are reported for the first time for cancer detection.
authors.affiliation: Department of Chemistry, MOE Key Lab of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Chemistry, MOE Key Lab of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, China.
authors.forename: Shudi
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, MOE Key Lab of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, China; State Key Laboratory of Marine Environmental Science, Xiamen University, China. Electronic address: weihang@xmu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Hang
authors.affiliation: Department of Urology, Xiamen First Hospital, China.
authors.forename: Yuedong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Urology, Xiamen First Hospital, China.
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Urology, Xiamen First Hospital, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Urology, Xiamen First Hospital, China.
authors.forename: Jinchun
authors.initials: J
authors.lastname: Xing
authors.affiliation: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, MOE Key Lab of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, China.
authors.forename: Eryi
authors.initials: E
authors.lastname: Zhu
authors.affiliation: Department of Chemistry, MOE Key Lab of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Yan
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D002292:Carcinoma, Renal Cell / Q000097:blood* / Q000473:pathology; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D016002:Discriminant Analysis; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000097:blood* / Q000473:pathology; D016018:Least-Squares Analysis; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000097:blood*; D011237:Predictive Value of Tests; D011379:Prognosis; D040901:Proteomics / Q000379:methods*; D012372:ROC Curve; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D014408:Biomarkers, Tumor; D010455:Peptides
keywords: Liquid chromatography–mass spectrometry; Peptide biomarkers; Renal clear cell carcinoma; Serum peptidomics; Unsupervised hierarchical cluster analysis