title: HLA DQB1 alleles are related with nonalcoholic fatty liver disease.
abstract: Nonalcoholic fatty liver disease (NAFLD) is the leading cause of chronic liver disease. NAFLD is a complex disease and inflammation is a crucial component in the disease pathogenesis. Recent genome wide association studies in hepatology area highlighted significant relations with human leukocyte antigen (HLA) DQ region and certain liver diseases. The previous animal models also emphasized the involvement of adaptive immune system in the liver damage pathways. To investigate possible polymorphisms in the HLA region that can contribute to the immune response affecting the NAFLD, we enrolled 93 consecutive biopsy proven NAFLD patients and a control group consisted of 101 healthy people and genotyped HLA DQB1 alleles at high resolution by sequence specific primers-polymerase chain reaction. The mean NAFLD activity score (NAS) was 5.2 ± 1.2, fibrosis score was 0.9 ± 0.9, ALT was 77 ± 47.4 U/L, AST was 49.4 ± 26.3 U/L. Among 13 HLA DQB1 alleles analyzed in this study, DQB1*06:04 was observed significantly at a more frequent rate among the NAFLD patients compared to that of healthy controls (12.9 vs. 2 % χ(2) = 8.6, P = 0.003, P c = 0.039, OR: 7.3 95 % CI 1.6-33.7). In addition, the frequency of DQB1*03:02 was significantly higher in the healthy control group than the NAFLD patients (24.8 vs. 7.5 %, χ(2) = 10.4, P = 0.001, P c = 0.013, OR: 0.2, 95 % CI 0.1-0.6). NAFLD patients were grouped according to their fibrosis score and NAS. The distribution of DQB1 alleles over stratified NAFLD patients did not reveal any statistically significant relation. Taken together, immune repertoire of individuals may have an effect on NAFLD pathogenesis and therefore, in NAFLD, adaptive immunity pathways should be investigated.
authors.affiliation: Department of Gastroenterology, Goztepe Teaching and Research Hospital, Medeniyet University, Istanbul, Turkey, levent.doganay@ueh.gov.tr.
authors.forename: Levent
authors.initials: L
authors.lastname: Doganay
authors.forename: Seyma
authors.initials: S
authors.lastname: Katrinli
authors.forename: Yasar
authors.initials: Y
authors.lastname: Colak
authors.forename: Ebubekir
authors.initials: E
authors.lastname: Senates
authors.forename: Ebru
authors.initials: E
authors.lastname: Zemheri
authors.forename: Oguzhan
authors.initials: O
authors.lastname: Ozturk
authors.forename: Feruze Yilmaz
authors.initials: FY
authors.lastname: Enc
authors.forename: Ilyas
authors.initials: I
authors.lastname: Tuncer
authors.forename: Gizem Dinler
authors.initials: GD
authors.lastname: Doganay
mesh_terms: D000328:Adult; D000483:Alleles; D005260:Female; D020022:Genetic Predisposition to Disease; D059866:HLA-DQ beta-Chains / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D065626:Non-alcoholic Fatty Liver Disease / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen