title: Expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and peroxiredoxin 6 (Prdx6) proteins in healthy and pathologic placentas of human and rat.
abstract: A relationship has been shown between preeclampsia (PE) and intrauterine growth restriction (IUGR) and oxidative stress (OS). Since such pregnancies experience OS, we aimed to detect the distribution pattern and expression levels of a transcription factor, Nuclear factor erythroid 2-related factor-2 (Nrf2) that has a role in the regulation of antioxidant enzymes, and peroxiredoxin 6 (Prdx6) an antioxidant enzyme, in human healthy, IUGR, PE and in groups of rat healthy and IUGR placentas using immunohistochemistry and Western blotting. Both Nrf2 and Prdx6 immunoreactivities were weaker in human and rat IUGR group placentas compared to human and rat control group placentas, respectively. Nrf2 and Prdx6 were immunostained in labyrinth trophoblasts, decidua, giant, glycogen and fetal vessel endothelial cells in rat control and IUGR group placentas. Nrf2 and Prdx6 immunoreactivities were seen in the decidua, syncytiotrophoblasts, villous stromal cells, and vascular endothelium in human control, IUGR and PE group placentas. Results of Nrf2 and Prdx6 Western blotting applied for rat and human placentas were compatible with the results of Nrf2 and Prdx6 immunohistochemical observations with regard to rat and human placentas. Down-regulation of Nrf2 and Prdx6 proteins in human and rat IUGR group placentas may have led to the formation of OS which may have impaired proliferation and invasion of cytotrophoblasts.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
authors.forename: Nuray
authors.initials: N
authors.lastname: Acar
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Soylu
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
authors.forename: Imren
authors.initials: I
authors.lastname: Edizer
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Ozbey
authors.affiliation: Department of Biophysics, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Er
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
authors.forename: Gokhan
authors.initials: G
authors.lastname: Akkoyunlu
authors.affiliation: Department of Physiology, Faculty of Medicine, Near East University, Nicosia, Mersin 10, Turkey.
authors.forename: Burcu
authors.initials: B
authors.lastname: Gemici
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey. Electronic address: iustunel@akdeniz.edu.tr.
authors.forename: Ismail
authors.initials: I
authors.lastname: Ustunel
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003656:Decidua / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism; D005260:Female; D006801:Humans; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D054465:Peroxiredoxin VI / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D051381:Rats; D014327:Trophoblasts / Q000378:metabolism
chemical_list: D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; C495637:Nfe2l2 protein, rat; D054465:Peroxiredoxin VI
keywords: Human; IUGR; Nrf2; PE; Placenta; Prdx6; Rat