title: Green synthesis, characterization and anti-inflammatory activity of silver nanoparticles using European black elderberry fruits extract.
abstract: This research aimed at reporting the synthesis, characterization and evaluation of the anti-inflammatory effects of some new biomaterials based on silver nanoparticles and polyphenols rich natural extracts. A fast and eco-friendly extracellular biosynthesis of silver nanoparticles (AgNPs), using European black elderberry (Sambucus nigra - SN, Adoxaceae family) fruit extracts was developed. The phytosynthesized nanoparticles exhibited an absorbance peak at 426nm, characteristic for AgNPs and their sizes were ranged from 20 to 80nm. The anti-inflammatory properties of AgNPs were assessed in vitro on HaCaT cells exposed to UVB radiation, in vivo on acute inflammation model and in humans on psoriasis lesions. In vitro, our results demonstrated the anti-inflammatory effects of functionalized AgNPs by the decrease of cytokines production induced by UVB irradiation. In vivo, the pre-administration of AgNPs reduced the edema and cytokines levels in the paw tissues, early after the induction of inflammation. The present study also demonstrated the possible use of synthesized AgNPs for the treatment of psoriasis lesions.
authors.affiliation: Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University, 11 Arany Janos Street, RO 400028, Cluj-Napoca, Romania.
authors.forename: Luminita
authors.initials: L
authors.lastname: David
authors.affiliation: Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University, 11 Arany Janos Street, RO 400028, Cluj-Napoca, Romania.
authors.forename: Bianca
authors.initials: B
authors.lastname: Moldovan
authors.affiliation: National Institute for Research and Development of Isotopic and Molecular Technologies, 65-103 Donath Street, RO 400293, Cluj-Napoca, Romania.
authors.forename: Adriana
authors.initials: A
authors.lastname: Vulcu
authors.affiliation: National Institute for Research and Development of Isotopic and Molecular Technologies, 65-103 Donath Street, RO 400293, Cluj-Napoca, Romania.
authors.forename: Liliana
authors.initials: L
authors.lastname: Olenic
authors.affiliation: "Ion Chiricuta" Oncology Institute, 34-36 Republicii Street, 400015, Cluj-Napoca, Romania. Electronic address: mperde@iocn.ro.
authors.forename: Maria
authors.initials: M
authors.lastname: Perde-Schrepler
authors.affiliation: "Ion Chiricuta" Oncology Institute, 34-36 Republicii Street, 400015, Cluj-Napoca, Romania.
authors.forename: Eva
authors.initials: E
authors.lastname: Fischer-Fodor
authors.affiliation: Department of Cell and Molecular Biology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 6 Louis Pasteur Street, 400349, Cluj Napoca, Romania.
authors.forename: Adrian
authors.initials: A
authors.lastname: Florea
authors.affiliation: Histology Department, "Iuliu Hatieganu" University of Medicine and Pharmacy, 13 Emil Isaac Street, 400023, Cluj-Napoca, Romania.
authors.forename: Maria
authors.initials: M
authors.lastname: Crisan
authors.affiliation: Faculty of Mathematics and Computer Science, Babes-Bolyai University, 1 Kogălniceanu Street, 400084, Cluj-Napoca, Romania.
authors.forename: Ioana
authors.initials: I
authors.lastname: Chiorean
authors.affiliation: Physiology Department, "Iuliu Hatieganu" University of Medicine and Pharmacy, 13 Emil Isaac Street, 400023, Cluj Napoca, Romania.
authors.forename: Simona
authors.initials: S
authors.lastname: Clichici
authors.affiliation: Physiology Department, "Iuliu Hatieganu" University of Medicine and Pharmacy, 13 Emil Isaac Street, 400023, Cluj Napoca, Romania. Electronic address: gabriela.filip@umfcluj.ro.
authors.forename: Gabriela Adriana
authors.initials: GA
authors.lastname: Filip
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002461:Cell Line, Transformed; D006801:Humans; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission; D010936:Plant Extracts / Q000494:pharmacology*; D027923:Sambucus / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D012834:Silver
keywords: Adoxaceae family; Anti-inflammatory effect; European black elderberry (Sambucus nigra); Psoriasis; Silver nanoparticles