title: Quantitative and qualitative evaluation of CART-containing cells in adrenal glands of male rats with hypertension.
abstract: Adrenal activity is stimulated and secretion of stress hormones is increased during advanced stages of renovascular hypertension. The literature suggests that the neuropeptide, cocaine and amphetamine regulated transcript (CART), might regulate adrenal secretory function and thus could influence its activity. We assessed potential quantitative and qualitative changes in the cells that contained CART in the adrenal glands of rats with renovascular hypertension. The renal arteries of ten rats were subjected to a clipping procedure, i.e., two-kidney one-clip (2K1C) model of arterial hypertension, and after 6 weeks each rat developed stable hypertension. CART was localized using immunohistochemistry. CART was detected in a large population of cells in the medulla, sparse nerve fibers in the cortex and the capsule of the adrenal gland. The population of CART-positive cells in adrenal glands of two kidney-one clip (2K1C) treated rats was greater and their immunoreactivity was increased compared to controls. Similarly, the length, width, area and diameter of CART-immunoreactive cells were significantly greater in the hypertensive rats than in controls. We demonstrated that renovascular hypertension alters the number and immunoreactivity of CART-containing cells in adrenal glands.
authors.affiliation: Department of Histology and Cytophysiology Medical University of Białystok ul . Mickiewicza 2C, 15-222 Białystok.
authors.forename: I
authors.initials: I
authors.lastname: Kasacka
authors.forename: Ż
authors.initials: Ż
authors.lastname: Piotrowska
authors.forename: M
authors.initials: M
authors.lastname: Knaś
authors.forename: A
authors.initials: A
authors.lastname: Lewandowska
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism* / Q000473:pathology*; D000818:Animals; D001794:Blood Pressure; D001835:Body Weight; D002452:Cell Count; D006978:Hypertension, Renovascular / Q000378:metabolism* / Q000473:pathology*; D007150:Immunohistochemistry; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D016707:Tissue Fixation; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
chemical_list: D009419:Nerve Tissue Proteins; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
keywords: CART; adrenal glands; hypertension; immunohistochemistry; rat