title: Effects of fullerenol nanoparticles on acetamiprid induced cytoxicity and genotoxicity in cultured human lung fibroblasts.
abstract: The aim of this study was to investigate the effects of water soluble fullerene (fullerenol) nanoparticles on the in vitro genotoxicity induced by the insecticide acetamiprid. Healthy human lung cells (IMR-90) were treated with fullerenol C60(OH)n (n: 18-22) alone and in combination with acetamiprid for 24h. The micronucleus test, comet assay and γ-H2AX foci formation assays were used as genotoxicity endpoints. Cytotoxicity was evaluated using the clonogenic assay. The maximum tested concentration of fullerenol (1.600 μg/ml) induced 77% survival where as the lowest concentration (25 μg/ml) was not cytotoxic where as acetamiprid was cytotoxic. Fullerenol did not induce genotoxicity at tested concentrations (50-1600 μg/L). On the other hand, acetamiprid (>50 μM) significantly induced formation of micronuclei, and double and single stranded DNA breaks in IMR-90 cells. For simultaneous exposure studies, two non-cytotoxic concentrations (50 and 200 μg/ml) of fullerenol and three cytotoxic concentrations of acetamiprid (100, 200 and 400 μM) were selected. As a result, we observed that co-exposure with fullerenol significantly reduced the cytotoxicity and genotoxicity of acetamiprid in IMR-90 cells. Our results indicated the protective effect of water soluble fullerene particles on herbicide induced genotoxicity.
authors.affiliation: Cell Culture and Genetic Toxicology Laboratory, Department of Biology, Faculty of Sciences and Arts, Uludag University, 16059 Nilüfer, Bursa, Turkey. Electronic address: tcavas@uludag.edu.tr.
authors.forename: Tolga
authors.initials: T
authors.lastname: Çavaş
authors.affiliation: Cell Culture and Genetic Toxicology Laboratory, Department of Biology, Faculty of Sciences and Arts, Uludag University, 16059 Nilüfer, Bursa, Turkey.
authors.forename: Nilüfer
authors.initials: N
authors.lastname: Çinkılıç
authors.affiliation: Cell Culture and Genetic Toxicology Laboratory, Department of Biology, Faculty of Sciences and Arts, Uludag University, 16059 Nilüfer, Bursa, Turkey.
authors.forename: Özgür
authors.initials: Ö
authors.lastname: Vatan
authors.affiliation: Cell Culture and Genetic Toxicology Laboratory, Department of Biology, Faculty of Sciences and Arts, Uludag University, 16059 Nilüfer, Bursa, Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Yılmaz
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D005347:Fibroblasts / Q000187:drug effects; D037741:Fullerenes / Q000494:pharmacology*; D006657:Histones / Q000378:metabolism; D006801:Humans; D007306:Insecticides / Q000633:toxicity*; D008168:Lung / Q000166:cytology; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D053758:Nanoparticles / Q000633:toxicity*; D000073943:Neonicotinoids; D020011:Protective Agents / Q000494:pharmacology*; D011725:Pyridines / Q000633:toxicity*
chemical_list: D037741:Fullerenes; C468783:H2AX protein, human; D006657:Histones; D007306:Insecticides; D009153:Mutagens; D000073943:Neonicotinoids; D020011:Protective Agents; D011725:Pyridines; C108127:fullerenol; C464485:acetamiprid
keywords: Acetamiprid; Cytotoxicity; Fullerenol; Genotoxicity; IMR-90 cells