title: Development of a lateral flow immunoassay strip for rapid detection of CagA antigen of Helicobacter pylori.
abstract: About half of the world populations are known to be infected with Helicobacter pylori. The CagA antigen secreting strains provoke severe mucosal damages and act as a risk factor for the development of peptic ulceration and gastric cancer. A lateral flow immunoassay (LFIA) strip was developed based on sandwich format for rapid detection of CagA antigen of H. pylori using gold conjugated monoclonal antibody. This LFIA strip will provide a good aid in the diagnosis of CagA-secreting H. pylori within 10 min instead of time consuming, expensive and laborious invasive approaches.
authors.affiliation: a Department of Biology , Fatih University, Buyukcekmece , Istanbul , Turkey.
authors.forename: Cebrail
authors.initials: C
authors.lastname: Karakus
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry* / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology*; D001426:Bacterial Proteins / Q000032:analysis / Q000276:immunology*; D016481:Helicobacter Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori
keywords: CagA antigen; Helicobacter pylori; colloidal gold; lateral flow immunoassay strip