title: Serum concentrations of anthraquinones after intake of Folium Sennae and potential modulation on P-glycoprotein.
abstract: Folium Sennae (leaves of Cassia angustifolia or senna) is a laxative and a component in diets for weight control. It contains a variety of anthranoids such as sennosides, aloe-emodin, and rhein. In order to measure the serum concentrations of senna anthranoids, Sprague-Dawley rats were orally administered with single dose and multiple doses of Folium Sennae. The concentrations of anthranoids in serum were determined by HPLC method before and after hydrolysis with sulfatase and β-glucuronidase. The results showed that in the serum, aloe-emodin glucuronides and rhein glucuronides were the major metabolites. Traces of rhein free form were present transiently during the early phase, whereas the free form of aloe-emodin was not detected. We also evaluated the modulation effect of Folium Sennae on P-glycoprotein by using the LS 180 cell model which showed that it significantly inhibited P-glycoprotein by 16-46 %. In conclusion, senna anthranoids were rapidly and extensively metabolized to rhein glucuronides and aloe-emodin glucuronides in rats. Folium Sennae ingestion inhibited the efflux function of P-glycoprotein in the intestine.
authors.affiliation: School of Pharmacy, China Medical University, Taichung, Taiwan, R. O. C.
authors.forename: Yu-Hsuan
authors.initials: YH
authors.lastname: Peng
authors.affiliation: School of Pharmacy, China Medical University, Taichung, Taiwan, R. O. C.
authors.forename: Shiuan-Pey
authors.initials: SP
authors.lastname: Lin
authors.affiliation: School of Pharmacy, China Medical University, Taichung, Taiwan, R. O. C.
authors.forename: Chung-Ping
authors.initials: CP
authors.lastname: Yu
authors.affiliation: School of Pharmacy, China Medical University, Taichung, Taiwan, R. O. C.
authors.forename: Shang-Yuan
authors.initials: SY
authors.lastname: Tsai
authors.affiliation: School of Pharmacy, China Medical University, Taichung, Taiwan, R. O. C.
authors.forename: Min-Yu
authors.initials: MY
authors.lastname: Chen
authors.affiliation: School of Pharmacy, China Medical University, Taichung, Taiwan, R. O. C.
authors.forename: Yu-Chi
authors.initials: YC
authors.lastname: Hou
authors.affiliation: School of Pharmacy, China Medical University, Taichung, Taiwan, R. O. C.
authors.forename: Pei-Dawn Lee
authors.initials: PD
authors.lastname: Chao
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000037:antagonists & inhibitors / Q000097:blood*; D000284:Administration, Oral; D000818:Animals; D000880:Anthraquinones / Q000097:blood* / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects; D020719:Glucuronides / Q000097:blood / Q000378:metabolism; D006801:Humans; D008297:Male; D018515:Plant Leaves; D010946:Plants, Medicinal / Q000737:chemistry; D017207:Rats, Sprague-Dawley; D029846:Senna Plant / Q000737:chemistry
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D000880:Anthraquinones; D020719:Glucuronides; C518327:aloe emodin; C020491:rhein