title: [Effects of hippocampal stimulus on α₅ subunit of extrasynaptic GABA(A) receptor in kainic acid-induced epileptic rats].
abstract: OBJECTIVE
  To observe the effects of electrical hippocampal stimulation of α₅ subunit of extrasynaptic GABA(A) receptor in kainic acid-induced epileptic rats, explore the optimal therapeutic parameters and elucidate the possible mechanism of electrical stimulation for hippocampal epilepsy.
  
  
  METHODS
  A total of 40 healthy male Sprague Dawley (SD) rats were randomly divided into 5 groups of epilepsy, sham operation, low-frequency stimulation (LFS), high-frequency stimulation (HFS) and normal control (n = 8 each). The expression of α₅ subunit of extrasynaptic GABA(A) receptor as well as the association with the curative effects of LFS were evaluated by behavioristics, real-time fluorogenic quantitative polymerase chain reaction (PCR) and Western blot.
  
  
  RESULTS
  The kainic acid kindling rats had class V attack with a modeling success rate of 67%. After treatment, as compared with sham operation and epilepsy groups, the number of seizures decreased significantly in LFS and HFS treatment groups (P < 0.01). But LFS and HFS treatment groups were compared, the number of seizures has no statistical significance (P > 0.05). When sham operation and epilepsy groups were compared, the number of seizures was not statistically significant (P > 0.05). The paired inter-group comparisons showed that LFS and HFS treatment groups and sham operation and epilepsy groups had significant differences in α₅ subunit mRNA and protein (P < 0.01). No statistical significance existed between LFS and HFS treatment groups (P > 0.05) or sham operation and HFS treatment groups (P > 0.05). As compared with normal control group, LFS treatment and HFS groups had significant difference (P < 0.01).
  
  
  CONCLUSIONS
  Electrical hippocampal stimulation can effectively inhibit seizures. Both LFS and HFS are equally effective, but the former offers greater benefits. And an inhibition of extrasynaptic α₅ subunit plays an important role in seizure during hippocampal stimulation.
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Xinxiang Medical University, Xinxiang 453100, China.
authors.forename: Fazheng
authors.initials: F
authors.lastname: Shen
authors.forename: Lei
authors.initials: L
authors.lastname: Hui
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Wang
authors.forename: Xinli
authors.initials: X
authors.lastname: Zhao
authors.forename: Wenke
authors.initials: W
authors.lastname: Zhou
authors.forename: Xinzhong
authors.initials: X
authors.lastname: Zhang
authors.forename: Guangming
authors.initials: G
authors.lastname: Yang
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Email: suntao@nxmu.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
mesh_terms: D000818:Animals; D004558:Electric Stimulation; D004827:Epilepsy / Q000139:chemically induced / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007608:Kainic Acid / Q000633:toxicity*; D007696:Kindling, Neurologic; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000378:metabolism*; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism*
chemical_list: D011963:Receptors, GABA-A; D007608:Kainic Acid