title: Lethal protein in mass consumption edible mushroom Agrocybe aegerita linked to strong hepatic toxicity.
abstract: Edible mushrooms are well-known for their health and nutritional benefits, however, undesirable effects have been reported in animals fed with these types of edible mushrooms (Nieminen et al., 2009). For health and safety reason, it is necessary to evaluate the toxicity of edible mushrooms, especially those that have been artificially cultured in recent decades. The aim of this study was to assess the safety of the edible mushroom Agrocybe aegerita, which is also known as Agrocybe cylindracea in Europe and America. Components from A. aegerita (Yt) were extracted in water and unexpectedly displayed lethal effect and median lethal dose (LD50) at 8.77 g/kg. Strong hepatic toxicity in BALB/c mice was observed when mice were administered with 25 and 250 mg/kg body weight/day of Yt for 6 days. To identify the hepatotoxic components, Yt was further separated into two components by Diaion HP-20 column chromatography to produce the proteins (Yp) and small molecules (Ys) fractions. Biochemical and histopathological analysis showed that Yp could induce liver injury. LC-MS/MS analysis of Yp identified the main causative agent as AAL (A. aegerita lectin), which was shown to have similar hepatotoxicity in the Yt and Yp fractions. In addition, proteinase treatment assays indicated that AAL is resistant to the degradation by digestive enzymes. We have shown that the strong hepatic toxicity is due to a lectin in A. aegerita. This study suggests that correct consumption of A. aegerita can avoid human health risk and help us better understand its nutritional and medicinal value.
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei Province, PR China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei Province, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Che
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei Province, PR China.
authors.forename: Yalin
authors.initials: Y
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei Province, PR China.
authors.forename: Guojun
authors.initials: G
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei Province, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei Province, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei Province, PR China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei Province, PR China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei Province, PR China.
authors.forename: Shrestha
authors.initials: S
authors.lastname: Alok
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei Province, PR China.
authors.forename: Yijie
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei Province, PR China.
authors.forename: Barry Hon Cheung
authors.initials: BH
authors.lastname: Wong
authors.affiliation: Wuhan University Hospital, Wuhan University, Wuhan, 430072, Hubei Province, PR China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei Province, PR China; Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Wuhan University, Wuhan, 430072, Hubei Province, PR China; Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan, 430068, Hubei Province, PR China. Electronic address: sunhui@whu.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
mesh_terms: D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology*; D002853:Chromatography, Liquid; D005260:Female; D005656:Fungal Proteins / Q000008:administration & dosage / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D009145:Mushroom Poisoning / Q000150:complications*; D053719:Tandem Mass Spectrometry
chemical_list: D005656:Fungal Proteins
keywords: Agrocybe aegerita; Hepatotoxicity; Lectin AAL; Stability; Total protein