title: Freedom of expression: cell-type-specific gene profiling.
abstract: Cell fate and behavior are results of differential gene regulation, making techniques to profile gene expression in specific cell types highly desirable. Many methods now enable investigation at the DNA, RNA and protein level. This review introduces the most recent and popular techniques, and discusses key issues influencing the choice between these such as ease, cost and applicability of information gained. Interdisciplinary collaborations will no doubt contribute further advances, including not just in single cell type but single-cell expression profiling.
authors.affiliation: The Gurdon Institute and Department of Physiology, Development, and Neuroscience, University of Cambridge, Cambridge, UK.
authors.forename: Leo
authors.initials: L
authors.lastname: Otsuki
authors.forename: Seth W
authors.initials: SW
authors.lastname: Cheetham
authors.forename: Andrea H
authors.initials: AH
authors.lastname: Brand
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002469:Cell Separation; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D009928:Organ Specificity / Q000235:genetics*