title: Role of the OB-fold of RNA helicase A in the synthesis of HIV-1 RNA.
abstract: RNA helicase A (RHA), a DExD/H protein, contains a stretch of repeated arginine and glycine-glycine (RGG) residues and an oligonucleotide/oligosaccharide-binding fold (OB-fold) at the C-terminus. RHA has been reported to function as a transcriptional cofactor. This study shows the role of RGG and OB-fold domains of RHA in the activation of transcription and splicing of HIV-1 RNA. RHA stimulates HIV-1 transcription by enhancing the occupancy of RNA polymerase II on the proviral DNA. Deletion of RGG or both RGG and OB-fold does not change the transcriptional activity of RHA, nor does the stability of viral RNA. However, deletion of both RGG and OB-fold rather than deletion of RGG only results in less production of multiply spliced 6D RNAs. The results suggest that the OB-fold is involved in modulating HIV-1 RNA splicing in the context of some HIV-1 strains while it is dispensable for the activation of HIV-1 transcription.
authors.affiliation: Lady Davis Institute for Medical Research and McGill AIDS Centre, Jewish General Hospital, Montreal, Quebec, Canada; Department of Medicine, McGill University, Montreal, Quebec, Canada. Electronic address: xingli107@gmail.com.
authors.forename: Li
authors.initials: L
authors.lastname: Xing
authors.affiliation: Lady Davis Institute for Medical Research and McGill AIDS Centre, Jewish General Hospital, Montreal, Quebec, Canada; Department of Medicine, McGill University, Montreal, Quebec, Canada.
authors.forename: Meijuan
authors.initials: M
authors.lastname: Niu
authors.affiliation: Lady Davis Institute for Medical Research and McGill AIDS Centre, Jewish General Hospital, Montreal, Quebec, Canada; Department of Medicine, McGill University, Montreal, Quebec, Canada. Electronic address: lawrence.kleiman@mcgill.ca.
authors.forename: Lawrence
authors.initials: L
authors.lastname: Kleiman
mesh_terms: D001665:Binding Sites; D053487:DEAD-box RNA Helicases / Q000737:chemistry* / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral; D057809:HEK293 Cells; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000378:metabolism*; D009841:Oligonucleotides / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D017510:Protein Folding; D017434:Protein Structure, Tertiary / Q000502:physiology; D012326:RNA Splicing; D012367:RNA, Viral / Q000096:biosynthesis*
chemical_list: D009363:Neoplasm Proteins; D009841:Oligonucleotides; D009844:Oligosaccharides; D012367:RNA, Viral; C098487:DHX9 protein, human; D053487:DEAD-box RNA Helicases
keywords: HIV-1; OB-fold; RNA helicase A; Splicing; Transcription