title: Involvement of ATP synthase β subunit in chikungunya virus entry into insect cells.
abstract: Chikungunya virus (CHIKV), the virus responsible for the disease chikungunya fever in humans, is transmitted by Aedes mosquitoes. While significant progress has been made in understanding the process by which CHIKV enters into mammalian cells, far less progress has been made in understanding the CHIKV entry process in insect cells. This study sought to identify mosquito-cell-expressed CHIKV-binding proteins through a combination of virus overlay protein binding assays (VOPBA) and mass spectroscopy. A 50-kDa CHIKV-binding protein was identified as the ATP synthase β subunit (ATPSβ). Co-immunoprecipitation studies confirmed the interaction, and colocalization analysis showed cell-surface and intracellular co-localization between CHIKV and ATPSβ. Both antibody inhibition and siRNA-mediated downregulation experiments targeted to ATPSβ showed a significant reduction in viral entry and virus production. These results suggest that ATPSβ is a CHIKV-binding protein capable of mediating the entry of CHIKV into insect cells.
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Phuttamonthol Sai 4, Nakhon Pathom, Bangkok, 73170, Thailand.
authors.forename: Chanida
authors.initials: C
authors.lastname: Fongsaran
authors.forename: Krit
authors.initials: K
authors.lastname: Jirakanwisal
authors.forename: Atichat
authors.initials: A
authors.lastname: Kuadkitkan
authors.forename: Nitwara
authors.initials: N
authors.lastname: Wikan
authors.forename: Phitchayapak
authors.initials: P
authors.lastname: Wintachai
authors.forename: Chutima
authors.initials: C
authors.lastname: Thepparit
authors.forename: Sukathida
authors.initials: S
authors.lastname: Ubol
authors.forename: Narumon
authors.initials: N
authors.lastname: Phaonakrop
authors.forename: Sittiruk
authors.initials: S
authors.lastname: Roytrakul
authors.forename: Duncan R
authors.initials: DR
authors.lastname: Smith
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D002646:Chikungunya virus / Q000502:physiology*; D015870:Gene Expression; D054884:Host-Pathogen Interactions; D013058:Mass Spectrometry; D025261:Mitochondrial Proton-Translocating ATPases / Q000378:metabolism*; D011485:Protein Binding; D011991:Receptors, Virus / Q000378:metabolism*; D053585:Virus Attachment; D053586:Virus Internalization
chemical_list: C506526:ATP5F1B protein, human; D011991:Receptors, Virus; D025261:Mitochondrial Proton-Translocating ATPases