title: Evaluation of nutritional and sub-acute toxicological study of plant based supplement of Achyranthes aspera.
abstract: The present study was conducted for the nutritional, microbiological and toxicological evaluation of test compound having main ingredient Achyranthes aspera. Nutritional value assessment, microbiological analysis and toxicological studies were conducted according to the standard reported methods which exhibited that A. aspera contains moisture 4.05%, proteins 20.54%, fats 0.903%, ash 20.25%, carbohydrates 54,26% and energy 294 Kcal. Vitamin profile was found to be B(1) 0.27mg/100g, B(2) 0.28mg/100g, B(3) 0.58mg/100g, B(6) 0.27mg/100g and B(9) 39μg/100g. The content of sodium, calcium, magnesium, potassium, chloride and phosphorus was found to be 1119.67, 5385.23, 5446.08, 1343.6, 675880.73 and 1447.5mg/kg respectively and trace metals i.e. iron, copper, zinc, manganese and aluminum were detected as 283.05, 8.062, 48.37, 16.12 and 9.853 mg/kg respectively. The microbiological result indicated that the compound qualifies the international standards of microbial limit and was found free from Salmonella species. The toxicological study was conducted to find safe use of Achyranthes aspera compound in human as a nutritive supplement in blood disorders. The toxicity studies exhibited that the test compound has a good effect on general health as an increase in body weights of animals of test group was noticed as compared to that of control group. Blood parameters before and after the study were monitored which confirms our hypothesis by showing an increase in hemoglobin from 9.133 to 10.96, RBC count from 3.11 to 3.6, WBC count from 5.68 to 5.73 and platelets from 245 to 319.
authors.affiliation: Pharmaceutical Research Centre PCSIR Labs Complex, Karachi, Pakistan.
authors.forename: Nudrat
authors.initials: N
authors.lastname: Fatima
authors.affiliation: Food and Marine Resources Research Centre, PCSIR Labs Complex, Karachi, Pakistan.
authors.forename: Nabeela G
authors.initials: NG
authors.lastname: Dar
authors.affiliation: Pharmaceutical Research Centre PCSIR Labs Complex, Karachi, Pakistan.
authors.forename: Hina
authors.initials: H
authors.lastname: Imran
authors.affiliation: Pharmaceutical Research Centre PCSIR Labs Complex, Karachi, Pakistan.
authors.forename: Tehmina
authors.initials: T
authors.lastname: Sohail
authors.affiliation: Food and Marine Resources Research Centre, PCSIR Labs Complex, Karachi, Pakistan.
authors.forename: Uzma
authors.initials: U
authors.lastname: Asghar
authors.affiliation: Pharmaceutical Research Centre PCSIR Labs Complex, Karachi, Pakistan.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Yaqeen
authors.affiliation: Department of Chemistry, University of Karachi, Karachi, Pakistan.
authors.forename: Shazia
authors.initials: S
authors.lastname: Syed
authors.affiliation: Food and Marine Resources Research Centre, PCSIR Labs Complex, Karachi, Pakistan.
authors.forename: Khalid
authors.initials: K
authors.lastname: Jamil
mesh_terms: D029044:Achyranthes / Q000737:chemistry / Q000382:microbiology / Q000633:toxicity; D000818:Animals; D019587:Dietary Supplements; D051379:Mice; D009753:Nutritive Value; D051381:Rats; D017208:Rats, Wistar