title: Deregulation of Bmi-1 is associated with enhanced migration, invasion and poor prognosis in salivary adenoid cystic carcinoma.
abstract: BACKGROUND
  Bmi-1 had been found to involve in self renewal of stem cells and tumorigenesis in various malignancies. In this study, we investigated the role of Bmi-1 in the development of salivary adenoid cystic carcinoma (SACC).
  
  
  METHODS
  At first, we confirmed that the deregulation of Bmi-1 was a frequent event in SACC; up-regulation of Bmi-1 was correlated with clinical stages, vital status and distant metastasis and associated with reduced overall survival and disease free survival. SACC-LM cells, higher migration and invasion abilities, elevated the expression of Bmi-1 protein, epithelial-mesenchymal transition (EMT) related proteins (Snail, Slug and Vimentin) and cancer stem cells (CSCs) related proteins (ABCG2, Notch, ALDH-1, Oct-4, Nanog and Epcam) compared to the SACC-83 cells (lower migration and invasion abilities). The migration and invasion abilities were inhibited in SACC-LM cells upon Bmi-1 knockdown. Meanwhile, Bmi-1 knockdown resulted in simultaneous loss of stem cell markers and EMT markers in SACC-LM cells.
  
  
  CONCLUSIONS
  Our studies confirm that Bmi-1 deregulation plays an important role in the development of SACC and contributes to the migration and the invasion abilities of SACC, which is involved in EMT and CSCs.
  
  
  CONCLUSIONS
  To our knowledge, this is the first study revealing that Bmi-1 deregulation is associated with enhanced migration, invasion and poor prognosis in salivary adenoid cystic carcinoma.
authors.affiliation: State Key Laboratory of Oncology in Southern China, Sun Yat-sen University Cancer Center, 510060 Guangzhou, Guangdong, P.R. China; Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Sun Yat-Sen University, 510080 Guangzhou, Guangdong, P.R. China.
authors.forename: Boyang
authors.initials: B
authors.lastname: Chang
authors.affiliation: State Key Laboratory of Oncology in Southern China, Sun Yat-sen University Cancer Center, 510060 Guangzhou, Guangdong, P.R. China.
authors.forename: Su
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Sun Yat-Sen University, 510080 Guangzhou, Guangdong, P.R. China.
authors.forename: Qianting
authors.initials: Q
authors.lastname: He
authors.affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Sun Yat-Sen University, 510080 Guangzhou, Guangdong, P.R. China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Sun Yat-Sen University, 510080 Guangzhou, Guangdong, P.R. China.
authors.forename: Luodan
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Sun Yat-Sen University, 510080 Guangzhou, Guangdong, P.R. China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Sun Yat-Sen University, 510080 Guangzhou, Guangdong, P.R. China. Electronic address: anxunwang@yahoo.com.
authors.forename: Anxun
authors.initials: A
authors.lastname: Wang
keywords: Bmi-1; Metastasis; Salivary adenoid cystic carcinoma