title: Xenografting of testicular tissue pieces: 12 years of an in vivo spermatogenesis system.
abstract: Spermatogenesis is a dynamic and complex process that involves endocrine and testicular factors. During xenotransplantation of testicular tissue fragments into immunodecifient mice, a functional communication between host brain and donor testis is established. This interaction allows for the progression of spermatogenesis and recovery of fertilisation-competent spermatozoa from a broad range of mammalian species. In the last few years, significant progress has been achieved in testis tissue xenografting that improves our knowledge about the factors determining the success of grafting. The goal of this review is to provide up to date information about the role of factors such as donor age, donor species, testis tissue preservation or type of recipient mouse on the efficiency of this technique. Applications are described and compared with other techniques with similar purposes. Recent work has demonstrated that testicular tissue xenografting is used as a model to study gonadotoxicity of drugs and to obtain sperm from valuable young males.
authors.affiliation: Department of BiologyFaculty of Science, Universidad Autónoma de Madrid, C/Darwin 2, Madrid 28049, SpainDepartment of Comparative Biology and Experimental MedicineFaculty of Veterinary Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada lucia.arregui@gmail.es.
authors.forename: Lucía
authors.initials: L
authors.lastname: Arregui
authors.affiliation: Department of BiologyFaculty of Science, Universidad Autónoma de Madrid, C/Darwin 2, Madrid 28049, SpainDepartment of Comparative Biology and Experimental MedicineFaculty of Veterinary Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada.
authors.forename: Ina
authors.initials: I
authors.lastname: Dobrinski
mesh_terms: D000367:Age Factors; D000818:Animals; D006085:Graft Survival; D006801:Humans; D008297:Male; D016513:Mice, SCID; D020127:Recovery of Function; D013045:Species Specificity; D013091:Spermatogenesis; D013094:Spermatozoa / Q000276:immunology / Q000378:metabolism / Q000637:transplantation*; D013737:Testis / Q000254:growth & development / Q000276:immunology / Q000378:metabolism / Q000637:transplantation*; D023001:Transplantation Tolerance; D014183:Transplantation, Heterologous