title: Improvement of Biodesulfurization Rate of Alginate Immobilized Rhodococcus erythropolis R1.
abstract: BACKGROUND
  Sulfur oxides released from the burning of oil causes severe environmental pollution. The sulfur can be removed via the 4S pathway in biodesulfurization (BDS). Immobilization approaches have been developed to prevent cell contamination of oil during the BDS process.
  
  
  OBJECTIVE
  The encapsulation of Rhodococcus erythropolis R1 in calcium alginate beads was studied in order to enhance conversion of dibenzothiophene (DBT) to 2-hydroxy biphenyl (2-HBP) as the final product. Also the effect of different factors on the BDS process was investigated.
  
  
  METHODS
  Calcium alginate capsules were prepared using peristaltic pumps with different needle sizes to control the beads sizes. Scanning electron microscopy and flow cytometry methods were used to study the distribution and viability of encapsulated cells, respectively. Two non-ionic surfactants and also nano Ƴ-Al2O3were used with the ratio of 0.5% (v/v) and 1:5 (v/v) respectively to investigate their BDS efficiency. In addition, the effect of different bead sizes and different concentrations of sodium alginate in BDS activity was studied.
  
  
  RESULTS
  The 2% (w/v) sodium alginate beads with 1.5mm size were found to be the optimum for beads stability and efficient 2-HBP production. The viability of encapsulated cells decreased by 12% after 20 h of desulfurization, compared to free cells. Adding the non-ionic surfactants markedly enhanced the rate of BDS, because of increasing mass transfer of DBT to the gel matrix. In addition, Span 80 was more effective than Tween 80. The nanoƳ-Al2O3 particles could increase BDS rate by up to two-folds greater than that of the control beads.
  
  
  CONCLUSIONS
  The nano Ƴ-Al2O3 can improve the immobilized biocatalyst for excellent efficiency of DBT desulfurization. Also the BDS activity can be enhanced by setting the other explained factors at optimum levels.
authors.affiliation: Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, IR Iran.
authors.forename: Peyman
authors.initials: P
authors.lastname: Derikvand
authors.affiliation: Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, IR Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Etemadifar
keywords: Alginate; Flow Cytometry; Immobilization; Nano particles; Optimization