title: The comparison of methods to identify the presence of fibrocytes in formalin-fixed, paraffin-embedded archival cardiac tissue with coronary heart disease.
abstract: The purpose of this study was to find the optimal technical approach to identify the presence of fibrocytes in formalin-fixed, paraffin-embedded archival cardiac tissue with CHD (coronary heart disease). Using the coexpression markers CD45 and αSMA, the presence of fibrocytes was examined by three different methods, including double immunohistochemistry staining, combination labeling of immunohistochemistry and immunofluorescence and double immunofluorescence labeling. Double immunohistochemistry staining was very difficult to identify the CD45(+)/αSMA(+) fibrocytes. Although combination staining of immunohistochemistry and immunofluorescence has made it possible to evaluate the co-localization of CD45 and αSMA in the fibrocytes, this method was prone to produce many false positive cells. In contrast, CD45(+)/αSMA(+) fibrocytes could be clearly recognized by double immunofluorescence labeling. In conclusion, double immunofluorescence labeling is the optimal technical approach to identify the presence of fibrocytes in routinely processed cardiac tissue with CHD.
authors.affiliation: Kunming Medical University, Kunming, Yunnan Province, China.
authors.forename: Pu-Ping
authors.initials: PP
authors.lastname: Lei
authors.affiliation: Yunnan Judicial Police Professional College, Kunming, Yunnan Province, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Shuai
authors.affiliation: Kunming Medical University, Kunming, Yunnan Province, China.
authors.forename: Shang-Wen
authors.initials: SW
authors.lastname: Wang
authors.affiliation: The Fourth Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China.
authors.forename: Si-Ming
authors.initials: SM
authors.lastname: Tao
authors.affiliation: Kunming Medical University, Kunming, Yunnan Province, China.
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Qu
authors.affiliation: Kunming Medical University, Kunming, Yunnan Province, China. Electronic address: wangdianhuakm@126.com.
authors.forename: Dian-Hua
authors.initials: DH
authors.lastname: Wang
mesh_terms: D000199:Actins / Q000378:metabolism; D003327:Coronary Disease / Q000378:metabolism / Q000473:pathology*; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology*; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D017493:Leukocyte Common Antigens / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D016612:Paraffin Embedding; D016707:Tissue Fixation
chemical_list: D000199:Actins; D017493:Leukocyte Common Antigens
keywords: Coronary heart disease; Fibrocytes; Human