title: MSC attenuate diabetes-induced functional impairment in adipocytes via secretion of insulin-like growth factor-1.
abstract: The function of subcutaneous adipocytes in promoting wound healing is significantly suppressed in diabetic wounds. Recent studies have demonstrated the ability of mesenchymal stem cell (MSC) to ameliorate impaired diabetic wound healing. We hypothesized that MSC function may involve subcutaneous adipocytes. The abnormal function of subcutaneous adipocytes from STZ induced diabetic mice including glucose uptake and free fatty acid (FFA) secretion level were assessed. Then these cells were co-cultured with MSC via a transwell system to observe the changes of metabolic index and glucose transporter four (GLUT4) as well as phosphoinositide 3-kinase/protein kinase (PI3K/AKT) signaling pathway expression. The results of metabolic index suggest that MSC obviously attenuated the diabetes-induced functional impairment. Both mRNA and protein expression analyses showed that PI3K/AKT insulin signaling pathway and GLUT4 expression were up-regulated. These changes were substantially associated with a increased level of insulin-like growth factor-1 (IGF-1) secretion from MSC. These findings suggest that MSC could attenuate abnormal function of diabetic adipocytes by IGF-1secretion, which was more or less associated with the beneficial effects of MSC on improving diabetic wound healing.
authors.affiliation: Key Laboratory of Wound Repair and Regeneration of PLA, The First Affiliated Hospital, General Hospital of PLA, Beijing 100048, PR China; Nanfang Hospital, Southern Medical University, Guangzhou 510515, PR China; Department of Oncology, Dongtai People's Hospital, Dongtai 224200, PR China.
authors.forename: Dongyun
authors.initials: D
authors.lastname: Gao
authors.affiliation: Nanfang Hospital, Southern Medical University, Guangzhou 510515, PR China; Graduate School of Tianjin Medical University, Tianjin 300052, PR China.
authors.forename: Jiangfan
authors.initials: J
authors.lastname: Xie
authors.affiliation: Nanfang Hospital, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Wound Repair and Regeneration of PLA, The First Affiliated Hospital, General Hospital of PLA, Beijing 100048, PR China; Nanfang Hospital, Southern Medical University, Guangzhou 510515, PR China; Department of Thoracic and Cardiovascular Surgery, Peking University People's Hospital, Peking University, Beijing 100044, PR China.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Feng
authors.affiliation: Nanfang Hospital, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yao
authors.affiliation: Nanfang Hospital, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Kui
authors.initials: K
authors.lastname: Ma
authors.affiliation: Key Laboratory of Wound Repair and Regeneration of PLA, The First Affiliated Hospital, General Hospital of PLA, Beijing 100048, PR China; Nanfang Hospital, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Li
authors.affiliation: Nanfang Hospital, Southern Medical University, Guangzhou 510515, PR China. Electronic address: 13926402695@126.com.
authors.forename: Xu
authors.initials: X
authors.lastname: Wu
authors.affiliation: Key Laboratory of Wound Repair and Regeneration of PLA, The First Affiliated Hospital, General Hospital of PLA, Beijing 100048, PR China; Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Sciences, General Hospital of PLA, Beijing 100853, PR China; Hainan Branch of the Chinese PLA General Hospital, Sanya, Hainan Province 572014, PR China. Electronic address: stellarahuang@sina.com.
authors.forename: Sha
authors.initials: S
authors.lastname: Huang
authors.affiliation: Key Laboratory of Wound Repair and Regeneration of PLA, The First Affiliated Hospital, General Hospital of PLA, Beijing 100048, PR China; Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Sciences, General Hospital of PLA, Beijing 100853, PR China. Electronic address: fuxiaobing@vip.sina.com.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Fu
mesh_terms: D017667:Adipocytes / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology*; D007328:Insulin / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D013311:Streptozocin
chemical_list: D017931:DNA Primers; D007328:Insulin; D013311:Streptozocin; D007334:Insulin-Like Growth Factor I
keywords: Adipocytes; Conditioned medium (CM); IGF-1; MSC; Wound