title: Enhanced dibenzothiophene biodesulfurization by immobilized cells of Brevibacterium lutescens in n-octane-water biphasic system.
abstract: In this study, it was the first report that the Brevibacterium lutescens CCZU12-1 was employed as a sulfur removing bacteria. Using dibenzothiophene (DBT) as the sole sulfur source, B. lutescens could selectively degrade DBT into 2-hydroxybiphenyl (2-HBP) via the "4S" pathway. In the basal salt medium (BSM) supplemented with 0.25 mM DBT and 0.5 g/L Tween-80, high desulfurization rate (100 %) was obtained by growth cells after 60 h. Furthermore, the n-octane-water (10:90, v/v) biphasic system was built for the biodesulfurization by resting cells. Moreover, a combination of magnetic nano Fe3O4 particles with calcium alginate immobilization was used for enhancing biodesulfurization. In this n-octane-water biphasic system, immobilized B. lutescens cells could be reused for not less than four times. Therefore, B. lutescens CCZU12-1 shows high potential in the biodesulfurization.
authors.affiliation: School of Chemical and Biological Engineering, Yancheng Institute of Technology, Yancheng, 224051, People's Republic of China, daiyong_yc@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Dai
authors.forename: Rong
authors.initials: R
authors.lastname: Shao
authors.forename: Gang
authors.initials: G
authors.lastname: Qi
authors.forename: Bin-Bin
authors.initials: BB
authors.lastname: Ding
mesh_terms: D055162:Biocatalysis; D001951:Brevibacterium / Q000166:cytology* / Q000378:metabolism*; D018914:Cells, Immobilized / Q000378:metabolism; D009795:Octanes / Q000737:chemistry*; D013455:Sulfur / Q000737:chemistry*; D013876:Thiophenes / Q000737:chemistry* / Q000378:metabolism*; D014867:Water / Q000737:chemistry*
chemical_list: D009795:Octanes; D013876:Thiophenes; D014867:Water; D013455:Sulfur; C026728:octane; C016366:dibenzothiophene