title: MicroRNA-145 inhibits cell proliferation by directly targeting ADAM17 in hepatocellular carcinoma.
abstract: MicroRNAs (miRNAs) are key regulators in cell processes. Emerging evidence has suggested that there is a direct association between miRNAs and cancer. However, the exact regulatory mechanisms of miRNAs in tumorigenesis are poorly understood. In the present study, we showed that miR-145 is able to significantly reduce mRNA and protein expression levels of A disintegrin and metalloproteinase 17 (ADAM17) in liver cancer cells (SMMC-7721, BEL-7402 and Huh-7). Dual luciferase reporter assays confirmed that ADAM17 is a direct target of miR-145. Notably, we found that miR-145 inhibits cell proliferation and growth activity in SMMC-7721 cells. These results demonstrated that it may be exert the function of tumor suppression in a particular link of cancer cell growth. Further studies revealed that the silencing of ADAM17 decreased the proliferation and growth activity of SMMC-7721 cells. Moreover, it reduced the expression of MMP-9. In conclusion, miR-145 inhibits liver cancer cell proliferation by directly targeting ADAM17. Thus, it may become a promising biological target in the treatment strategy of hepatocellular carcinoma.
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Yuwu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pathology, Shenzhen Sixth People's Hospital (Nanshan Hospital), Shenzhen, Guangdong 518052, P.R. China.
authors.forename: Chang
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Sailan
authors.initials: S
authors.lastname: Wen
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Junpu
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Qiongqiong
authors.initials: Q
authors.lastname: He
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Deyun
authors.initials: D
authors.lastname: Feng
mesh_terms: D051722:ADAM Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D000072198:ADAM17 Protein; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D057809:HEK293 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D051722:ADAM Proteins; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9; D000072198:ADAM17 Protein; C000606462:ADAM17 protein, human