title: Effect of Scutia buxifolia Reissek in nucleotidase activities and inhibition of platelet aggregation.
abstract: This study aimed to determine the effect of lyophilized aqueous extracts of Scutia buxifolia on NTPDase and 5'-nucleotidase activity on platelets and lymphocytes as well as the profile of the platelet aggregation. In vitro tests were used to investigate the effect of the aqueous crude extract obtained from S. buxifolia leaves (SbL) and stem bark (SbS) on enzymatic activities and platelet aggregation. The platelets and lymphocytes were exposed to lyophilized aqueous extracts of S. buxifolia at concentrations of 1-200 μg/mL in the presence of ATP, ADP, AMP as substrates, during the enzymatic assay, as well as the platelet aggregation. The results showed that SbS and SbL potently inhibited NTPDase and 5'-nucleotidase in platelets and lymphocytes. ADP-induced aggregation was inhibited by the SbS (50, 100, and 200 μg/mL) and SbL (200 μg/mL). In addition, these results suggest that S. buxifolia have compounds, such as gallic acid, chlorogenic acid, caffeic acid, quercetin, rutin, and kaempferol, which cause a decrease the NTPDase and 5'-nucleotidase activity, resulting in alterations in adenine nucleotide levels and protection against ADP-induced platelet aggregation.
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, Brazil, alineboligon@hotmail.com.
authors.forename: Aline Augusti
authors.initials: AA
authors.lastname: Boligon
authors.forename: Victor Camera
authors.initials: VC
authors.lastname: Pimentel
authors.forename: Margarete Dulce
authors.initials: MD
authors.lastname: Bagatini
authors.forename: Margareth Linde
authors.initials: ML
authors.lastname: Athayde
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism*; D000818:Animals; D001081:Apyrase / Q000378:metabolism*; D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology; D017208:Rats, Wistar; D027823:Rhamnaceae / Q000737:chemistry*; D012431:Rutin / Q000494:pharmacology
chemical_list: D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D012431:Rutin; D011794:Quercetin; D015720:5'-Nucleotidase; D001081:Apyrase