title: HPLC-DAD analysis, antileishmanial, antiproliferative, and antibacterial activities of Lacistema pubescens: an Amazonian medicinal plant.
abstract: Species of the genus Lacistema are traditionally used by Brazilian and Peruvian indigenous communities. The present study investigated the in vitro antileishmanial activity against several Leishmania species, cytotoxicity in murine peritoneal macrophages, antiproliferative activity against HL60 and Jurkat cells, and antibacterial activities against seven bacteria strains of the aerial parts of the methanolic crude extract and fractions of Lacistema pubescens. In addition, their chemical profile was also evaluated. Hexane fraction showed the most significant IC50 values against all promastigotes of Leishmania species tested, except for L. chagasi (IC50 = 4.2 µg/mL for L. major and IC50 = 3.5 µg/mL for L. amazonensis). This fraction also exhibited a strong activity against amastigotes of L. amazonensis (IC50 = 6.9 µg/mL). The antiproliferative activity was also observed for methanolic extract and hexane fraction with IC50 = 47.2 µg/mL and IC50 = 39.7 µg/mL for HL60, respectively. Regarding the antimicrobial activity, the overall antibacterial activity was not very significative. Phytol and sitosterol were identified in the methanolic extract. Additionally, previous studies also revealed the presence of those compounds in the hexane fraction. Among other compounds, phytol and sitosterol were probably involved in the antileishmanial and cytotoxicity activities observed in this study.
authors.affiliation: Bioactive Natural Products Laboratory, Department of Biochemistry, Biological Sciences Institute, Federal University of Juiz de Fora, José Lourenço Kelmer, s/n, São Pedro, 36036-900 Juiz de Fora, MG, Brazil.
authors.forename: Josiane Mello
authors.initials: JM
authors.lastname: da Silva
authors.affiliation: Department of Parasitology, Microbiology and Immunology, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: Luciana Maria Ribeiro
authors.initials: LM
authors.lastname: Antinarelli
authors.affiliation: Bioactive Natural Products Laboratory, Department of Biochemistry, Biological Sciences Institute, Federal University of Juiz de Fora, José Lourenço Kelmer, s/n, São Pedro, 36036-900 Juiz de Fora, MG, Brazil.
authors.forename: Nícolas de Castro Campos
authors.initials: Nde C
authors.lastname: Pinto
authors.affiliation: Department of Parasitology, Microbiology and Immunology, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: Elaine Soares
authors.initials: ES
authors.lastname: Coimbra
authors.affiliation: Department of Physiology and Biophysics, Biological Sciences Institute, Federal University of Minas Gerais, Avenida Antônio Carlos, 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: Elaine Maria
authors.initials: EM
authors.lastname: de Souza-Fagundes
authors.affiliation: Bioactive Natural Products Laboratory, Department of Biochemistry, Biological Sciences Institute, Federal University of Juiz de Fora, José Lourenço Kelmer, s/n, São Pedro, 36036-900 Juiz de Fora, MG, Brazil.
authors.forename: Antônia
authors.initials: A
authors.lastname: Ribeiro
authors.affiliation: Bioactive Natural Products Laboratory, Department of Biochemistry, Biological Sciences Institute, Federal University of Juiz de Fora, José Lourenço Kelmer, s/n, São Pedro, 36036-900 Juiz de Fora, MG, Brazil.
authors.forename: Elita
authors.initials: E
authors.lastname: Scio
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D001938:Brazil; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells; D006801:Humans; D019169:Jurkat Cells; D007891:Leishmania / Q000187:drug effects* / Q000502:physiology; D007928:Lethal Dose 50; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D010936:Plant Extracts / Q000008:administration & dosage*; D010946:Plants, Medicinal / Q000737:chemistry; D064028:Tracheophyta / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D010936:Plant Extracts