title: High-performance beating pattern function of human induced pluripotent stem cell-derived cardiomyocyte-based biosensors for hERG inhibition recognition.
abstract: High-throughput and high clinical relevance methods are demanded to predict the drug-induced cardiotoxicity in pharmaceutical and biotechnology industries to effectively decrease late-stage drug attrition. In this study, human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) were integrated into an interdigital impedance sensor array to fabricate a high performance iPSC-CM-based biosensor array with high-throughput and high-consistency beating pattern. Typical withdrawal approved drugs (astemizole, sertindole, cisapride, and droperidol) with hERG inhibition and positive control E-4031 were employed to determine the beating pattern function. From the results, it can be concluded that this iPSC-CM-based biosensor array can specifically differentiate the hERG inhibitors from the non-hERG inhibition compounds through beating pattern function.
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China. Electronic address: huning@zju.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Hu
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
authors.forename: Tianxing
authors.initials: T
authors.lastname: Wang
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zou
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
authors.forename: Kaiqi
authors.initials: K
authors.lastname: Su
authors.affiliation: ACEA Biosciences (Hangzhou) Co., Ltd., High Throughput Drug Screening Center, Xihu Technology and Economy Park, Hangzhou 310030, PR China.
authors.forename: Jieying
authors.initials: J
authors.lastname: Wu
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China. Electronic address: cnpwang@zju.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D001681:Biological Assay / Q000295:instrumentation; D015374:Biosensing Techniques / Q000295:instrumentation*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003217:Conductometry / Q000295:instrumentation*; D000072237:ERG1 Potassium Channel; D004867:Equipment Design; D019544:Equipment Failure Analysis; D051638:Ether-A-Go-Go Potassium Channels / Q000037:antagonists & inhibitors*; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D026902:Potassium Channel Blockers / Q000494:pharmacology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000072237:ERG1 Potassium Channel; D051638:Ether-A-Go-Go Potassium Channels; C000606913:KCNH2 protein, human; D026902:Potassium Channel Blockers
keywords: Beating pattern function; Drug cardiotoxicity assessment; Human iPSC-CM-based biosensor array; Impedance detection technology; hERG inhibitor recognition