title: [Analysis and identification of peptide epitope of the fifth stage larvae of Angiostrongylus cantonensis].
abstract: OBJECTIVE
  To identify the specific antigens of the fifth stage larvae (larvae V) of Angiostrongylus cantonensis in soluble proteins.
  
  
  METHODS
  The techniques of two-dimensional electrophoresis (2-DE) and immunoblot were employed to analyze the soluble components of larvae V antigens. The resulting protein spots were identified by mass spectrometry and the specific antigen and immunogenic peptide segment profiles determined.Larvae V was identified by immunoblot of peptide immune murine sera.
  
  
  RESULTS
  Immunoblot of 2-DE showed that there were 8 specific antigen spots in females and 4 in males of larvae V. The 12 protein spots had a pH range of 5-7 and a molecular weight of 24-40 kD. Among 12 spot, actin-1 and galectin-1 were identified successfully. And a peptide (NH2-KNGDIALHFNPRFDEK) was predicted as epitope and synthesized. The binding capacity of murine immune sera could identify the 32 000 of larvae V.
  
  
  CONCLUSIONS
  Galectin and actin are common antigens of female and male larvae V of A.cantonensis. And a peptide from galectin-1 may become a potential target for immunologic diagnosis and immunological mediator.
authors.affiliation: Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Huicong
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Yao
authors.affiliation: Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Zibo
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, China. Email: wzpcw@sohu.com.
authors.forename: Changwang
authors.initials: C
authors.lastname: Pan
mesh_terms: D017185:Angiostrongylus cantonensis; D000818:Animals; D000941:Antigens; D000939:Epitopes; D005260:Female; D007814:Larva; D008297:Male; D010455:Peptides; D011506:Proteins; D017206:Strongylida Infections
chemical_list: D000941:Antigens; D000939:Epitopes; D010455:Peptides; D011506:Proteins