title: Involvement of chromosome region maintenance 1 (CRM1) in the formation and progression of esophageal squamous cell carcinoma.
abstract: Chromosome region maintenance 1 (CRM1) has been related to several malignancies. The predictive value of CRM1 in the malignance and prognosis of esophageal squamous cell carcinoma (ESCC), however, is not clear yet. In this study, we displayed that CRM1 expression was up-regulated in ESCC using immunohistochemistry and Western blot. Statistical analysis demonstrated that patients with high CRM1 levels indicated shorter survival period. We further found that silencing CRM1 caused apoptosis in ESCC cell lines. Moreover, knockdown of CRM1 disturbed the expression of tumor suppressor proteins and inhibited NF-κB activity in ESCC cell lines, especially if the cell line was treated with 5-fluorouracil. In consequence, our results for the first time indicated that CRM1 was dysregulated in ESCC, and suppression of CRM1 expression which resulted in inhibiting of NF-κB signaling might be developed into a new strategy in ESCC therapy.
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Jiangsu, 226001, People's Republic of China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Yang
authors.forename: Lei
authors.initials: L
authors.lastname: Cheng
authors.forename: Li
authors.initials: L
authors.lastname: Yao
authors.forename: Hanru
authors.initials: H
authors.lastname: Ren
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.forename: Xiao
authors.initials: X
authors.lastname: Min
authors.forename: Xueyu
authors.initials: X
authors.lastname: Chen
authors.forename: Jianguo
authors.initials: J
authors.lastname: Zhang
authors.forename: Mei
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000378:metabolism* / Q000401:mortality; D045744:Cell Line, Tumor; D018450:Disease Progression; D004938:Esophageal Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000401:mortality; D000077277:Esophageal Squamous Cell Carcinoma; D004947:Esophagus / Q000737:chemistry / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D028884:Karyopherins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism; D011379:Prognosis; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D016019:Survival Analysis; D000097606:Exportin 1 Protein
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D028884:Karyopherins; D016328:NF-kappa B; D034741:RNA, Small Interfering; D018160:Receptors, Cytoplasmic and Nuclear; D000097606:Exportin 1 Protein