title: Serum levels of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinases-1 in human herpesvirus-6-infected infants with or without febrile seizures.
abstract: Human herpesvirus-6 (HHV-6) is a cause of exanthema subitum and, sometimes, of febrile seizures. However, the pathogenesis of febrile seizures associated with HHV-6 infection remains unclear. We investigated serum matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinases-1 (TIMP-1) levels in infants with HHV-6 infection. Serum levels of both MMP-9 and TIMP-1 were significantly higher in infants with HHV-6 infection than in controls. Serum TIMP-1 levels were significantly higher in infants with febrile seizures than in infants without febrile seizures. Serum MMP-9/TIMP-1 ratios were significantly lower in infants with febrile seizures than in infants without febrile seizures. In infants with HHV-6 infection, positive correlations were found between serum MMP-9 concentrations and the white blood cells (WBC) count, and between serum TIMP-1 concentrations and the WBC count. Positive correlations were also found between the amounts of HHV-6 DNA and the ratios of MMP-9/TIMP-1 in infants with HHV-6 infection. In conclusion, we suggest that high serum levels of MMP-9 and TIMP-1 in infants with HHV-6 infection may induce dysfunction of the blood-brain barrier, eventually causing febrile seizures.
authors.affiliation: Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan. Electronic address: setsuaki@yamaguchi-u.ac.jp.
authors.forename: Setsuaki
authors.initials: S
authors.lastname: Kittaka
authors.affiliation: Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan.
authors.forename: Shunji
authors.initials: S
authors.lastname: Hasegawa
authors.affiliation: Department of Pediatrics, Nagoya University Graduate School of Medicine, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Ito
authors.affiliation: Department of Pediatrics, Yamaguchi Red Cross Hospital, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Ohbuchi
authors.affiliation: Department of Pediatrics, Suzuki Pediatric Clinic, Japan.
authors.forename: Eitaro
authors.initials: E
authors.lastname: Suzuki
authors.affiliation: Department of Pediatrics, Shimonoseki City Hospital, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Kawano
authors.affiliation: Department of Pediatrics, Nagato General Hospital, Japan.
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Aoki
authors.affiliation: Department of Pediatrics, Toyoura Hospital, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Nakatsuka
authors.affiliation: Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Kudo
authors.affiliation: Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Wakiguchi
authors.affiliation: Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan.
authors.forename: Madoka
authors.initials: M
authors.lastname: Kajimoto
authors.affiliation: Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Matsushige
authors.affiliation: Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ichiyama
mesh_terms: D001812:Blood-Brain Barrier; D002675:Child, Preschool; D004279:DNA, Viral / Q000097:blood*; D005077:Exanthema Subitum / Q000097:blood* / Q000150:complications; D005260:Female; D015654:Herpesvirus 6, Human; D006801:Humans; D007223:Infant; D007958:Leukocyte Count; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D003294:Seizures, Febrile / Q000097:blood* / Q000150:complications; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood*
chemical_list: D004279:DNA, Viral; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D020780:Matrix Metalloproteinase 9
keywords: Blood–brain barrier; Exanthema subitum; Febrile seizures; Human herpesvirus-6; Matrix metalloproteinase-9; Tissue inhibitor of metalloproteinases-1