title: Necroptosis, in vivo detection in experimental disease models.
abstract: Over the last decade, our picture of cell death signals involved in experimental disease models totally shifted. Indeed, in addition to apoptosis, multiple forms of regulated necrosis have been associated with an increasing number of pathologies such as ischemia-reperfusion injury in brain, heart and kidney, inflammatory diseases, sepsis, retinal disorders, neurodegenerative diseases and infectious disorders. Especially necroptosis is currently attracting the attention of the scientific community. However, the in vivo identification of ongoing necroptosis in experimental disease conditions remains troublesome, mainly due to the lack of specific biomarkers. Initially, Receptor-Interacting Protein Kinase 1 (RIPK1) and RIPK3 kinase activity were uniquely associated with induction of necroptosis, however recent evidence suggests pleiotropic functions in cell death, inflammation and survival, obscuring a clear picture. In this review, we will present the last methodological advances for in vivo necroptosis identification and discuss past and recent data to provide an update of the so-called "necroptosis-associated pathologies".
authors.affiliation: Inflammation Research Center, Molecular Signaling and Cell Death Unit, VIB, Technologiepark 927, B-9052 Ghent, Belgium; Department for Biomedical Molecular Biology, Molecular Signaling and Cell Death Unit, Ghent University, Technologiepark 927, B-9052 Ghent, Belgium.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Jouan-Lanhouet
authors.affiliation: Department for Biomedical Molecular Biology, Molecular Signaling and Cell Death Unit, Ghent University, Technologiepark 927, B-9052 Ghent, Belgium; Laboratoire de Régulation des Signaux de Division, EA4479, Université Lille1, Villeneuve d'Ascq, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Riquet
authors.affiliation: Inflammation Research Center, Molecular Signaling and Cell Death Unit, VIB, Technologiepark 927, B-9052 Ghent, Belgium; Department for Biomedical Molecular Biology, Molecular Signaling and Cell Death Unit, Ghent University, Technologiepark 927, B-9052 Ghent, Belgium.
authors.forename: Linde
authors.initials: L
authors.lastname: Duprez
authors.affiliation: Inflammation Research Center, Molecular Signaling and Cell Death Unit, VIB, Technologiepark 927, B-9052 Ghent, Belgium; Department for Biomedical Molecular Biology, Molecular Signaling and Cell Death Unit, Ghent University, Technologiepark 927, B-9052 Ghent, Belgium.
authors.forename: Tom
authors.initials: T
authors.lastname: Vanden Berghe
authors.affiliation: Inflammation Research Center, Molecular Signaling and Cell Death Unit, VIB, Technologiepark 927, B-9052 Ghent, Belgium; Department for Biomedical Molecular Biology, Molecular Signaling and Cell Death Unit, Ghent University, Technologiepark 927, B-9052 Ghent, Belgium.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Takahashi
authors.affiliation: Inflammation Research Center, Molecular Signaling and Cell Death Unit, VIB, Technologiepark 927, B-9052 Ghent, Belgium; Department for Biomedical Molecular Biology, Molecular Signaling and Cell Death Unit, Ghent University, Technologiepark 927, B-9052 Ghent, Belgium; Methusalem Program, Ghent University, Technologiepark 927, B-9052 Ghent, Belgium. Electronic address: Peter.Vandenabeele@irc.vib-UGent.be.
authors.forename: Peter
authors.initials: P
authors.lastname: Vandenabeele
mesh_terms: D000818:Animals; D053181:Caspase 8 / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D009336:Necrosis / Q000378:metabolism*; D010338:Pathology, Clinical / Q000379:methods*; D011494:Protein Kinases / Q000378:metabolism; D053422:Receptor-Interacting Protein Serine-Threonine Kinases / Q000378:metabolism; D015398:Signal Transduction
chemical_list: C570693:MLKL protein, human; D011494:Protein Kinases; C506249:RIPK1 protein, human; C119705:RIPK3 protein, human; D053422:Receptor-Interacting Protein Serine-Threonine Kinases; D053181:Caspase 8
keywords: Experimental disease models; MLKL; Methods; Necroptosis; RIPK1/3