title: Protein sorting at the trans-Golgi network.
abstract: The trans-Golgi network (TGN) is an important cargo sorting station within the cell where newly synthesized proteins are packaged into distinct transport carriers that are targeted to various destinations. To maintain the fidelity of protein transport, elaborate protein sorting machinery is employed to mediate sorting of specific cargo proteins into distinct transport carriers. Protein sorting requires assembly of the cytosolic sorting machinery onto the TGN membrane and capture of cargo proteins. We review the cytosolic and transmembrane sorting machinery that function at the TGN and describe molecular interactions and regulatory mechanisms that enable accurate protein sorting. In addition, we highlight the importance of TGN sorting in physiology and disease.
authors.affiliation: Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, California 94720-3200; email: schekman@berkeley.edu.
authors.forename: Yusong
authors.initials: Y
authors.lastname: Guo
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Sirkis
authors.forename: Randy
authors.initials: R
authors.lastname: Schekman
mesh_terms: D020823:ADP-Ribosylation Factor 1 / Q000502:physiology; D033942:Adaptor Proteins, Vesicular Transport / Q000502:physiology; D020816:Amino Acid Motifs; D000818:Animals; D002352:Carrier Proteins / Q000502:physiology; D016764:Cell Polarity; D003600:Cytosol / Q000502:physiology; D006801:Humans; D008563:Membrane Lipids / Q000502:physiology; D026901:Membrane Transport Proteins / Q000502:physiology; D008954:Models, Biological; D008958:Models, Molecular; D010743:Phospholipids / Q000502:physiology; D011487:Protein Conformation; D021382:Protein Sorting Signals / Q000502:physiology; D021381:Protein Transport / Q000276:immunology / Q000502:physiology*; D013329:Structure-Activity Relationship; D022161:Transport Vesicles / Q000502:physiology; D033921:Vesicular Transport Proteins / Q000502:physiology; D021601:trans-Golgi Network / Q000276:immunology / Q000502:physiology*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D002352:Carrier Proteins; D008563:Membrane Lipids; D026901:Membrane Transport Proteins; D010743:Phospholipids; D021382:Protein Sorting Signals; D033921:Vesicular Transport Proteins; D020823:ADP-Ribosylation Factor 1
keywords: Arfs; cargo adaptors; cargo receptors; phospholipids; vesicle coat proteins