title: Physico-chemical and toxicological characterization of iron-containing albumin nanoparticles as platforms for medical imaging.
abstract: Iron oxide-containing magnetic nanoparticles (MNPs) have certain advantages over currently used contrast agents for tumor imaging by magnetic resonance imaging (MRI) as they offer the possibility of functionalization with ligands and tracers. Functionalized MNPs also may be used for targeted tumor therapy. In the current study nanoparticles (NPs) consisting of recombinant human serum albumin (rHSA) with incorporated hydrophilic (NH4)2Ce(IV)(NO3)6-γ-Fe2O3 particles (CAN maghemite particles) for medical imaging were produced and characterized. For this purpose CAN maghemite particles were incorporated into an rHSA matrix to yield rHSA-NPs. The resulting NPs were analyzed by transmission electron microscopy, photon correlation spectroscopy, and atomic absorption. The sizes of the manufactured NP were 170 ± 10 nm, and the zeta-potential was -50 ± 3 mV. The NPs remained stable when stored after lyophilization with sucrose 3% [w/v] as a cryoprotector. They showed pro-inflammatory properties without cell and animal toxicity in vivo and were highly contrasting in MRI. In conclusion, this report introduces novel rHSA NP with favorable properties containing iron oxide for detection by MRI.
authors.affiliation: Institute of Pharmaceutical Technology, Goethe University, 60438 Frankfurt/Main, Germany.
authors.forename: Ina
authors.initials: I
authors.lastname: Rosenberger
authors.affiliation: Department of Medicine 1, University Hospital Frankfurt, Goethe University, 60590 Frankfurt/Main, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Schmithals
authors.affiliation: Rega Institute for Medical Research, University of Leuven, KU Leuven, Belgium.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Vandooren
authors.affiliation: Fondazione Filarete, 20139 Milano, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bianchessi
authors.affiliation: Fondazione Filarete, 20139 Milano, Italy; CIMAINA, University of Milano, 20129 Milano, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Milani
authors.affiliation: Department of Industrial Chemistry Toso Montanari, University of Bologna, Bologna, Italy.
authors.forename: Erica
authors.initials: E
authors.lastname: Locatelli
authors.affiliation: Department of Chemistry & Institute of Nanotechnology & Advanced Materials, Bar-Ilan University, Ramat-Gan, 5290002, Israel.
authors.forename: Liron L
authors.initials: LL
authors.lastname: Israel
authors.affiliation: Department of Diagnostic and Interventional Radiology, University Hospital Frankfurt, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Hübner
authors.affiliation: Department of Diagnostic and Interventional Radiology, University Hospital Frankfurt, Germany.
authors.forename: Michela
authors.initials: M
authors.lastname: Matteoli
authors.affiliation: Department of Chemistry & Institute of Nanotechnology & Advanced Materials, Bar-Ilan University, Ramat-Gan, 5290002, Israel.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Lellouche
authors.affiliation: Department of Industrial Chemistry Toso Montanari, University of Bologna, Bologna, Italy.
authors.forename: Mauro Comes
authors.initials: MC
authors.lastname: Franchini
authors.affiliation: Fondazione Filarete, 20139 Milano, Italy.
authors.forename: Lorena
authors.initials: L
authors.lastname: Passoni
authors.affiliation: Fondazione Filarete, 20139 Milano, Italy.
authors.forename: Eugenio
authors.initials: E
authors.lastname: Scanziani
authors.affiliation: Rega Institute for Medical Research, University of Leuven, KU Leuven, Belgium.
authors.forename: Ghislain
authors.initials: G
authors.lastname: Opdenakker
authors.affiliation: Department of Medicine 1, University Hospital Frankfurt, Goethe University, 60590 Frankfurt/Main, Germany.
authors.forename: Albrecht
authors.initials: A
authors.lastname: Piiper
authors.affiliation: Institute of Pharmaceutical Technology, Goethe University, 60438 Frankfurt/Main, Germany. Electronic address: kreuter@em.uni-frankfurt.de.
authors.forename: Jörg
authors.initials: J
authors.lastname: Kreuter
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003287:Contrast Media / Q000008:administration & dosage / Q000633:toxicity; D003952:Diagnostic Imaging / Q000379:methods*; D004355:Drug Stability; D004563:Electrochemistry; D005290:Ferric Compounds / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity; D006801:Humans; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D058185:Magnetite Nanoparticles / Q000008:administration & dosage / Q000633:toxicity; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D009369:Neoplasms / Q000175:diagnosis; D010316:Particle Size; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000633:toxicity; D012709:Serum Albumin / Q000008:administration & dosage / Q000633:toxicity
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D011994:Recombinant Proteins; D012709:Serum Albumin; C000499:ferric oxide
keywords: Air pouch model; Contrast agent; Diagnostic tool; Irwin test; Magnetic nanoparticles; Medical imaging