title: Fullerene derived molecularly imprinted polymer for chemosensing of adenosine-5'-triphosphate (ATP).
abstract: For molecular imprinting of oxidatively electroactive analytes by electropolymerization, we used herein reductively electroactive functional monomers. As a proof of concept, we applied C60 fullerene adducts as such for the first time. For that, we derivatized C60 to bear either an uracil or an amide, or a carboxy addend for recognition of the adenosine-5'-triphosphate (ATP) oxidizable analyte with the ATP-templated molecularly imprinted polymer (MIP-ATP). Accordingly, the ATP complex with all of the functional monomers formed in solution was potentiodynamically electropolymerized to deposit an MIP-ATP film either on an Au electrode of the quartz crystal resonator or on a Pt disk electrode for the piezoelectric microgravimetry (PM) or capacitive impedimetry (CI) determination of ATP, respectively, under the flow-injection analysis (FIA) conditions. The apparent imprinting factor for ATP was ∼4.0. After extraction of the ATP template, analytical performance of the resulting chemosensors, including detectability, sensitivity, and selectivity, was characterized. The limit of detection was 0.3 and 0.03mM ATP for the PM and CI chemosensor, respectively. The MIP-ATP film discriminated structural analogues of ATP quite well. The Langmuir, Freundlich, and Langmuir-Freundlich isotherms were fitted to the experimental data of the ATP sorption and sorption stability constants appeared to be nearly independent of the adopted sorption model.
authors.affiliation: Department of Physical Chemistry of Supramolecular Complexes, Institute of Physical Chemistry, Polish Academy of Sciences (IPC PAS), Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Piyush S
authors.initials: PS
authors.lastname: Sharma
authors.affiliation: Department of Physical Chemistry of Supramolecular Complexes, Institute of Physical Chemistry, Polish Academy of Sciences (IPC PAS), Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Dabrowski
authors.affiliation: Department of Physical Chemistry of Supramolecular Complexes, Institute of Physical Chemistry, Polish Academy of Sciences (IPC PAS), Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Noworyta
authors.affiliation: Department of Physical Chemistry of Supramolecular Complexes, Institute of Physical Chemistry, Polish Academy of Sciences (IPC PAS), Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Tan-Phat
authors.initials: TP
authors.lastname: Huynh
authors.affiliation: Department of Chemistry, University of North Texas, 1155 Union Circle, #305070, Denton, TX 76203-5017, USA.
authors.forename: Chandra B
authors.initials: CB
authors.lastname: Kc
authors.affiliation: Department of Physical Chemistry of Supramolecular Complexes, Institute of Physical Chemistry, Polish Academy of Sciences (IPC PAS), Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Janusz W
authors.initials: JW
authors.lastname: Sobczak
authors.affiliation: Department of Physical Chemistry of Supramolecular Complexes, Institute of Physical Chemistry, Polish Academy of Sciences (IPC PAS), Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Pieta
authors.affiliation: Department of Chemistry, University of North Texas, 1155 Union Circle, #305070, Denton, TX 76203-5017, USA. Electronic address: francis.dsouza@unt.edu.
authors.forename: Francis
authors.initials: F
authors.lastname: D'Souza
authors.affiliation: Department of Physical Chemistry of Supramolecular Complexes, Institute of Physical Chemistry, Polish Academy of Sciences (IPC PAS), Kasprzaka 44/52, 01-224 Warsaw, Poland; Faculty of Mathematics and Natural Sciences, School of Science, Cardinal Stefan Wyszynski University in Warsaw, Wóycickiego 1/3, 01-815 Warsaw, Poland. Electronic address: wkutner@ichf.edu.pl.
authors.forename: Wlodzimierz
authors.initials: W
authors.lastname: Kutner
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry*; D037741:Fullerenes / Q000737:chemistry*; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*
chemical_list: D037741:Fullerenes; D011108:Polymers; D000255:Adenosine Triphosphate
keywords: Adenosine-5′-triphosphate; Capacitive impedimetry; Electroactive functional monomer; Fullerene polymer; Molecularly imprinted polymer; Piezoelectric microgravimetry