title: The kinesin KIF21B participates in the cell surface delivery of γ2 subunit-containing GABAA receptors.
abstract: KIF21B, a kinesin family (KIF) protein, is a plus end-directed microtubule motor. The KIF21B gene is highly expressed in neuronal tissue and spleen and is a susceptibility locus for multiple sclerosis. KIF21B motility is regulated through TRIM3, a member of the cytoskeleton-associated-recycling or transport (CART) complex, involved in vesicular receptor recycling. Here we show that the GABAA receptor γ2-subunit co-precipitates and co-localizes with KIF21B in cultured hippocampal neurons. Knockdown of KIF21B gene expression through small hairpin (sh) RNA reduces the number of γ2-subunit-containing GABAA receptor (GABAARs) clusters in neurites and at the cell surface. Our data suggest that KIF21B participates in the delivery of GABAAR transport vesicles into dendrites.
authors.affiliation: University Medical Center Hamburg-Eppendorf, Center for Molecular Neurobiology, ZMNH, Falkenried 94, 20251 Hamburg, Germany.
authors.forename: Dorthe
authors.initials: D
authors.lastname: Labonté
authors.affiliation: University Medical Center Hamburg-Eppendorf, Center for Molecular Neurobiology, ZMNH, Falkenried 94, 20251 Hamburg, Germany.
authors.forename: Edda
authors.initials: E
authors.lastname: Thies
authors.affiliation: University Medical Center Hamburg-Eppendorf, Center for Molecular Neurobiology, ZMNH, Falkenried 94, 20251 Hamburg, Germany. Electronic address: matthias.kneussel@zmnh.uni-hamburg.de.
authors.forename: Matthias
authors.initials: M
authors.lastname: Kneussel
mesh_terms: D000818:Animals; D002450:Cell Communication; D002462:Cell Membrane / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D006624:Hippocampus / Q000166:cytology; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000378:metabolism; D021122:Protein Subunits / Q000378:metabolism; D051381:Rats; D011963:Receptors, GABA-A / Q000378:metabolism*
chemical_list: C546280:Gabrg2 protein, mouse; D021122:Protein Subunits; D011963:Receptors, GABA-A; C550348:KIF21B protein, human; D016547:Kinesins
keywords: Cytoskeleton; GABA(A) receptor; KIF21B; Kinesin; Microtubule; Motor protein; Neuron; Synapse; Transport; γ2 subunit