title: [Association of C677T gene polymorphisms of methylenetetrahydrofolate reductase and plasma homocysteine level with hyperlipidemia].
abstract: OBJECTIVE
  To study the association of methylenetetrahydrofolate reductase (MTHFR) gene C677T mutation and plasma homocysteine (Hcy) levels with hyperlipidemia.
  
  
  METHODS
  Blood samples were collected from 1591 adults for detecting MTHFR gene C677T polymorphism with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), plasma Hcy levels with enzymatic cycling method, and blood lipid levels as well. The patients were divided according to the lipid levels into hyperlipidemia group (n=694) and healthy control group (n=897) and the differences in MTHFR gene C677T polymorphisms and plasma Hcy levels were compared.
  
  
  RESULTS
  The hyperlipidemia group and healthy control group showed no significant differences in CC, CT, or TT genotype frequencies or C and T allele frequencies of MTHFR C677T gene, and had comparable plasma Hcy levels (P>0.05). Patients with 3 different MTHFR C677T genotypes had significant differences in plasma Hcy levels (P<0.01) but not in blood lipid levels (P>0.05). Pairwise comparison indicated a significantly higher plasma Hcy level in TT genotype than in CC and CT genotypes (P<0.01), and the latter two genotypes showed no significant difference (P>0.05).
  
  
  CONCLUSIONS
  MTHFR C677T polymorphisms and plasma Hcy levels are closely related but neither of them is associated with hyperlipidemia. The TT genotype is associated with a significantly higher plasma Hcy level than CC and CT genotypes.
authors.affiliation: Clinical Laboratory, Shunde First People's Hospital Affiliated to Southern Medical University, Shunde 528300, China.E-mail: liangruilian2011@163.com.
authors.forename: Ruilian
authors.initials: R
authors.lastname: Liang
authors.forename: Yuanqing
authors.initials: Y
authors.lastname: Zhou
authors.forename: Jianmin
authors.initials: J
authors.lastname: Xie
authors.forename: Weibiao
authors.initials: W
authors.lastname: Lv
authors.forename: Bin
authors.initials: B
authors.lastname: Kang
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Liang
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Chen
authors.forename: Yunxi
authors.initials: Y
authors.lastname: Li
mesh_terms: D000328:Adult; D005787:Gene Frequency; D005838:Genotype; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood* / Q000235:genetics*; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D009154:Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D006710:Homocysteine; C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2)