title: Simultaneous determination of caffeic acid and its major pharmacologically active metabolites in rat plasma by LC-MS/MS and its application in pharmacokinetic study.
abstract: A simple, sensitive and selective high-performance liquid chromatography electrospray ionization tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination and pharmacokinetic study of caffeic acid (CA) and its active metabolites. The separation with isocratic elution used a mobile phase composed of methanol and water (containing 0.1% formic acid) at a flow rate of 0.2 mL/min. The detection of target compounds was done in selected reaction monitoring (SRM) mode. The SRM detection was operated in the negative electrospray ionization mode using the transitions m/z 179 ([M - H](-) ) → 135 for CA, m/z 193 ([M - H](-) ) → 134.8 for ferulic acid and isoferulic acid and m/z 153 ([M - H](-) ) → 108 for protocatechuic acid. The method was linear for all analytes over the investigated range with all correlation coefficients 0.9931. The lower limits of quantification were 5.0 ng/mL for analytes. The intra- and inter-day precisions (relative standard deviation) were <5.86 and <6.52%, and accuracy (relative error) was between -5.95 and 0.35% (n = 6). The developed method was applied to study the pharmacokinetics of CA and its major active metabolites in rat plasma after oral and intravenous administration of CA.
authors.affiliation: Tasly R&D Institute, Tianjin Tasly Group Co. Ltd, Tianjin, 300410, People's Republic of China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Ma
authors.forename: Yang
authors.initials: Y
authors.lastname: Chu
authors.forename: Shuming
authors.initials: S
authors.lastname: Li
authors.forename: Jiahua
authors.initials: J
authors.lastname: Guo
authors.forename: Yumeng
authors.initials: Y
authors.lastname: Jia
authors.forename: Shuiping
authors.initials: S
authors.lastname: Zhou
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Zhu
authors.forename: Changxiao
authors.initials: C
authors.lastname: Liu
mesh_terms: D000818:Animals; D002109:Caffeic Acids / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002109:Caffeic Acids; C040048:caffeic acid
keywords: LC-MS/MS; caffeic acid; metabolites; pharmacokinetics; simultaneous determination