title: Efficacy of lipopolysaccharide antigen of Yersinia ruckeri in rainbow trout by intraperitoneal and bath immersion administration.
abstract: In this study, Intraperitoneal (IP) and bath immersion (BI) vaccine trials were conducted in fish with a mean weight of 6.3 g. Rainbow trout vaccinated with lipopolysaccharide (LPS) was 50 mg/L protein concentration and challenged by IP injection with 9.8 × 10(6) cell/ml of Yersinia ruckeri at 45 days post-immunization had a relative percent survival (RPS). To obtain an effective bath immersion vaccine against yersiniosis, LPS preparation was obtained from the Y. ruckeri and with the LPS antigen. After 28 and 60 days vaccinated fish with first and second immunizations by LPS were challenged via intraperitoneal injection with 9.8 × 10(6) cell/ml of Y. ruckeri for evaluating the mortality rates and calculating the relative percentage of survival (RPS). RPS value of experimental groups, which was significantly (P < 0.05) larger than that of the control group.
authors.affiliation: Fisheries Faculty, Inonu University, Malatya, Turkey. Electronic address: uispir@yahoo.com.
authors.forename: Unal
authors.initials: U
authors.lastname: Ispir
authors.affiliation: Faculty of Fisheries, Firat University, Elazig, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Dorucu
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology / Q000627:therapeutic use*; D001494:Baths / Q000379:methods / Q000662:veterinary; D005393:Fish Diseases / Q000276:immunology / Q000401:mortality / Q000517:prevention & control*; D007274:Injections, Intraperitoneal / Q000379:methods / Q000662:veterinary; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use; D017686:Oncorhynchus mykiss / Q000276:immunology* / Q000382:microbiology; D015996:Survival Rate; D013997:Time Factors; D016896:Treatment Outcome; D015009:Yersinia Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D044124:Yersinia ruckeri / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D008070:Lipopolysaccharides
keywords: Bath immersion vaccine; Intraperitoneal vaccine; Lipopolysaccharide; Rainbow trout; Response; Yersinia ruckeri