title: Evidence against translational repression by the carboxyltransferase component of Escherichia coli acetyl coenzyme A carboxylase.
abstract: In Escherichia coli, synthesis of the malonyl coenzyme A (malonyl-CoA) required for membrane lipid synthesis is catalyzed by acetyl-CoA carboxylase, a large complex composed of four subunits. The subunit proteins are needed in a defined stoichiometry, and it remains unclear how such production is achieved since the proteins are encoded at three different loci. Meades and coworkers (G. Meades, Jr., B. K. Benson, A. Grove, and G. L. Waldrop, Nucleic Acids Res. 38:1217-1227, 2010, doi:http://dx.doi.org/10.1093/nar/gkp1079) reported that coordinated production of the AccA and AccD subunits is due to a translational repression mechanism exerted by the proteins themselves. The AccA and AccD subunits form the carboxyltransferase (CT) heterotetramer that catalyzes the second partial reaction of acetyl-CoA carboxylase. Meades et al. reported that CT tetramers bind the central portions of the accA and accD mRNAs and block their translation in vitro. However, long mRNA molecules (500 to 600 bases) were required for CT binding, but such long mRNA molecules devoid of ribosomes seemed unlikely to exist in vivo. This, plus problematical aspects of the data reported by Meades and coworkers, led us to perform in vivo experiments to test CT tetramer-mediated translational repression of the accA and accD mRNAs. We report that increased levels of CT tetramer have no detectable effect on translation of the CT subunit mRNAs.
authors.affiliation: Department of Microbiology, University of Illinois, Urbana, Illinois, USA.
authors.forename: Alexander C
authors.initials: AC
authors.lastname: Smith
authors.affiliation: Department of Microbiology, University of Illinois, Urbana, Illinois, USA Department of Biochemistry, University of Illinois, Urbana, Illinois, USA j-cronan@life.illinois.edu.
authors.forename: John E
authors.initials: JE
authors.lastname: Cronan
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D046188:Protein Modification, Translational / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D012333:RNA, Messenger; D000103:Acetyl-CoA Carboxylase