title: Effect of age, gender and mannose-binding lectin (MBL) status on the inflammatory profile in peripheral blood plasma of Australian blood donors.
abstract: Transfusion of blood components has been associated with poor patient outcomes and, an overall increase in morbidity and mortality. Differences in the blood components arising from donor health, age and immune status may impact on outcomes of transfusion and transfusion-related immune modulation in recipients. The aim of this study was to investigate differences in inflammatory profile in donors and association with parameters including age, gender and deficiency status of pattern recognition molecule mannose-binding lectin (MBL). MBL level was determined by ELISA. Serum levels of interleukin (IL)-1α, IL-1β, IL-6, IL-8, IL-10, IL-12, tumour necrosis factor (TNF)-α, macrophage inflammatory protein (MIP)-1α, monocyte chemoattractant protein (MCP)-1, interferon (IFN)-α, and IFN-γ were examined by cytometric bead array (CBA). C-reactive protein (CRP) and rheumatoid factor (RF) were examined by immunoturbidimetry. This study demonstrated age was a parameter associated with the immune profile of blood donors, with significant increases in MCP-1 (p<0.05) and RF (p<0.05) and decreases in IL-1α evident in the older donors (61-76years). Significant gender-associated differences in MCP-1, IL-12 and CRP plasma levels in the blood donor cohort were also reported. There was no significant difference in the level of any inflammatory markers studied according to MBL status. This study demonstrated that age and gender are associated with inflammatory profile in donors. These differences may be a factor impacting on outcomes of transfusion.
authors.affiliation: Research and Development, Australian Red Cross Blood Service, Brisbane, Australia; Faculty of Health, Queensland University of Technology, Brisbane, Australia.
authors.forename: Katrina
authors.initials: K
authors.lastname: Kildey
authors.affiliation: Research and Development, Australian Red Cross Blood Service, Brisbane, Australia.
authors.forename: Kelly
authors.initials: K
authors.lastname: Rooks
authors.affiliation: Faculty of Health, Queensland University of Technology, Brisbane, Australia.
authors.forename: Steven
authors.initials: S
authors.lastname: Weier
authors.affiliation: Research and Development, Australian Red Cross Blood Service, Brisbane, Australia; Faculty of Health, Queensland University of Technology, Brisbane, Australia.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Flower
authors.affiliation: Research and Development, Australian Red Cross Blood Service, Brisbane, Australia; Faculty of Health, Queensland University of Technology, Brisbane, Australia. Electronic address: meldean@redcrossblood.org.au.
authors.forename: Melinda M
authors.initials: MM
authors.lastname: Dean
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D001315:Australia; D001782:Blood Donors / Q000706:statistics & numerical data*; D002097:C-Reactive Protein / Q000378:metabolism; D018932:Chemokine CCL2 / Q000097:blood; D015331:Cohort Studies; D016207:Cytokines / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D018664:Interleukin-12 / Q000097:blood; D053582:Interleukin-1alpha / Q000097:blood; D016014:Linear Models; D037601:Mannose-Binding Lectin / Q000097:blood*; D008875:Middle Aged; D015999:Multivariate Analysis; D009391:Nephelometry and Turbidimetry; D012217:Rheumatoid Factor / Q000097:blood; D012737:Sex Factors; D055815:Young Adult
chemical_list: D018932:Chemokine CCL2; D016207:Cytokines; D018836:Inflammation Mediators; D053582:Interleukin-1alpha; D037601:Mannose-Binding Lectin; D018664:Interleukin-12; D002097:C-Reactive Protein; D012217:Rheumatoid Factor
keywords: Age; Blood donors; Gender; Inflammatory markers; Mannose-binding lectin