title: Characterization of the polycystic kidney disease 2 gene promoter.
abstract: The key regulatory elements for PKD2 transcription remain unclear. To identify these core elements, we characterized porcine PKD2 promoter with bioinformatics and molecular tools and found porcine PKD2 promoter bearing typical features of enriched CpG and less TATA. Further studies demonstrated that the core region was located in fragment -483 to +100. Subsequent biophysical binding assays and mutation experiments revealed that G4 motif and Sp1 are critical regulators for mediating the transcription of porcine PKD2. Moreover, the same regulatory pattern was reproduced in human PKD2 promoter region, indicating the critical role of G4 and Sp1 in regulating PKD2.
authors.affiliation: Research Centre of Basic Medical Science,Department of Cell Biology, Tianjin Medical University, Qixiangtai Road, Heping District, Tianjin 300070, China.
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Research Centre of Basic Medical Science,Department of Cell Biology, Tianjin Medical University, Qixiangtai Road, Heping District, Tianjin 300070, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Han
authors.affiliation: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Ye
authors.affiliation: Research Centre of Basic Medical Science,Department of Cell Biology, Tianjin Medical University, Qixiangtai Road, Heping District, Tianjin 300070, China.
authors.forename: Xianjun
authors.initials: X
authors.lastname: Gao
authors.affiliation: Research Centre of Basic Medical Science,Department of Cell Biology, Tianjin Medical University, Qixiangtai Road, Heping District, Tianjin 300070, China.
authors.forename: Hongjing
authors.initials: H
authors.lastname: Niu
authors.affiliation: Research Centre of Basic Medical Science,Department of Cell Biology, Tianjin Medical University, Qixiangtai Road, Heping District, Tianjin 300070, China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Research Centre of Basic Medical Science,Department of Cell Biology, Tianjin Medical University, Qixiangtai Road, Heping District, Tianjin 300070, China.
authors.forename: Yawen
authors.initials: Y
authors.lastname: Chai
authors.affiliation: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Research Centre of Basic Medical Science,Department of Cell Biology, Tianjin Medical University, Qixiangtai Road, Heping District, Tianjin 300070, China. Electronic address: haifangyin@tmu.edu.cn.
authors.forename: HaiFang
authors.initials: H
authors.lastname: Yin
mesh_terms: D000818:Animals; D001483:Base Sequence; D018899:CpG Islands; D057809:HEK293 Cells; D006801:Humans; D008969:Molecular Sequence Data; D059372:Nucleotide Motifs; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D016329:Sp1 Transcription Factor / Q000378:metabolism; D013552:Swine; D015533:Transcriptional Activation
chemical_list: D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D016329:Sp1 Transcription Factor; D017346:Protein Serine-Threonine Kinases
keywords: G-quadruplex; G4R1; PKD2; Sp1; Transcription