title: Solubilization of gliadins for use as a source of nitrogen in the selection of bacteria with gliadinase activity.
abstract: For patients with celiac disease, gliadin detoxification via the use of gliadinases may provide an alternative to a gluten-free diet. A culture medium, in which gliadins were the sole source of nitrogen, was developed for screening for microorganisms with gliadinase activity. The problem of gliadin insolubility was solved by mild acid treatment, which renders an acid-hydrolysed gliadin/peptide mixture (AHG). This medium provided a sensitive and reliable means of detecting proteases, compared to the classical spectrophotometric method involving azocasein. When a sample of fermented wheat (a source of bacteria) was plated on an AHG-based culture medium, strains with gliadinase activity were isolated. These strains' gliadinase profiles were determined using an AHG-based substrate in zymographic analyses.
authors.affiliation: Dairy Research Institute (IPLA-CSIC), 33300 Villaviciosa, Asturias, Spain. Electronic address: pas@ipla.csic.es.
authors.forename: Patricia
authors.initials: P
authors.lastname: Alvarez-Sieiro
authors.affiliation: Dairy Research Institute (IPLA-CSIC), 33300 Villaviciosa, Asturias, Spain. Electronic address: bredruel@ipla.csic.es.
authors.forename: Begoña
authors.initials: B
authors.lastname: Redruello
authors.affiliation: Dairy Research Institute (IPLA-CSIC), 33300 Villaviciosa, Asturias, Spain. Electronic address: ladero@ipla.csic.es.
authors.forename: Victor
authors.initials: V
authors.lastname: Ladero
authors.affiliation: Dairy Research Institute (IPLA-CSIC), 33300 Villaviciosa, Asturias, Spain. Electronic address: elecfdez@gmail.com.
authors.forename: Elena
authors.initials: E
authors.lastname: Cañedo
authors.affiliation: Dairy Research Institute (IPLA-CSIC), 33300 Villaviciosa, Asturias, Spain. Electronic address: mcm@ipla.csic.es.
authors.forename: M Cruz
authors.initials: MC
authors.lastname: Martin
authors.affiliation: Dairy Research Institute (IPLA-CSIC), 33300 Villaviciosa, Asturias, Spain. Electronic address: mfernandez@ipla.csic.es.
authors.forename: María
authors.initials: M
authors.lastname: Fernández
authors.affiliation: Dairy Research Institute (IPLA-CSIC), 33300 Villaviciosa, Asturias, Spain. Electronic address: maag@ipla.csic.es.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Alvarez
mesh_terms: D001419:Bacteria / Q000201:enzymology*; D005903:Gliadin / Q000737:chemistry*; D006868:Hydrolysis; D009584:Nitrogen / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D012995:Solubility
chemical_list: D005903:Gliadin; D010447:Peptide Hydrolases; D009584:Nitrogen
keywords: Azocasein (PubChem CID: 39859); Culture medium; Cycloheximide (PubChem CID: 6197); Diethyl ethoxymethylenemalonate (PubChem CID: 6871); Gliadin (PubChem CID: 17787981); Gliadin solubility; Gliadinase; Gluten; Zymography