title: Effect of ligustrazine on levels of amino acid neurotransmitters in rat striatum after cerebral ischemia-reperfusion injury.
abstract: This study aimed to evaluate the effect of ligustrazine on levels of amino acid transmitters in the extracellular fluid of striatum following cerebral ischemia/reperfusion (I/R) in male Sprague-Dawley rats. A microdialysis cannula guide was implanted into the right striatum. After recovery, animals underwent a sham operation or middle cerebral artery occlusion (MCAO). Those that developed cerebral ischemia after MCAO were randomized to receive propylene glycol salt water and ligustrazine respectively. Striatal fluid samples were collected from all animals at 15-min intervals after treatment and were subjected to HPLC analysis of aspartic acid, glutamic acid, taurine, and γ-amino butyric acid. Upon the last sample collection, animals were sacrificed and brain tissue specimens were collected for triphenyltetrazolium chloride staining and NeuN staining. Compared with the sham operation, MCAO induced significant neurological deficits and increased striatal concentrations of the four neurotransmitters assessed in a time-dependent manner (P < 0.01). Ligustrazine effectively attenuated the detrimental effects of MCAO on the brain. These observations suggest that ligustrazine as a novel cerebral infarction-protective agent may have potential clinical implications for I/R-related brain damage.
authors.affiliation: a College of Bioengineering, Zhejiang Chinese Medical University , Hangzhou 310053 , China.
authors.forename: Jin
authors.initials: J
authors.lastname: Han
authors.forename: Hai-Tong
authors.initials: HT
authors.lastname: Wan
authors.forename: Jie-Hong
authors.initials: JH
authors.lastname: Yang
authors.forename: Yu-Yan
authors.initials: YY
authors.lastname: Zhang
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Ge
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Bie
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001224:Aspartic Acid / Q000032:analysis; D001921:Brain / Q000187:drug effects; D002545:Brain Ischemia / Q000188:drug therapy; D003342:Corpus Striatum; D018698:Glutamic Acid / Q000032:analysis; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy; D008297:Male; D017551:Microdialysis; D015394:Molecular Structure; D018377:Neurotransmitter Agents / Q000032:analysis; D011719:Pyrazines / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism; D013654:Taurine / Q000032:analysis; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000032:analysis
chemical_list: D000596:Amino Acids; D018377:Neurotransmitter Agents; D011719:Pyrazines; D013654:Taurine; D001224:Aspartic Acid; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; C017953:tetramethylpyrazine
keywords: amino acid neurotransmitters; cerebral ischemia-reperfusion; ligustrazine; middle cerebral artery occlusion; rats