title: Short communication: Effects of lactose and milk on the expression of biofilm-associated genes in Staphylococcus aureus strains isolated from a dairy cow with mastitis.
abstract: Staphylococcus aureus is the main etiological organism responsible for bovine mastitis. The ability of S. aureus to form biofilms plays an important role in the pathogenesis of mastitis. Biofilm formation in S. aureus is associated with the production of polysaccharide intercellular adhesin (PIA) protein and several other proteins. Several environmental factors, including glucose, osmolarity, oleic acid, temperature, and anaerobiosis, have been reported to affect bioﬁlm formation in S. aureus. This study investigated the influence of lactose and milk on the biofilm formation capacity of 2 clinical bovine isolates of S. aureus. We found that lactose increased biofilm formation predominantly by inducing PIA production, whereas milk increased biofilm formation through PIA as well as by increasing the production of other biofilm-associated proteins, which might be mediated by the transcriptional regulators intercellular adhesion regulator (icaR) and repressor of biofilm (rbf).
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, China. Electronic address: xuet@ahau.edu.cn.
authors.forename: Ting
authors.initials: T
authors.lastname: Xue
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Shang
mesh_terms: D000818:Animals; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002417:Cattle; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007785:Lactose / Q000494:pharmacology*; D008414:Mastitis, Bovine / Q000382:microbiology*; D008892:Milk / Q000502:physiology*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D011135:Polysaccharides, Bacterial; C113579:polysaccharide intercellular adhesin; D007785:Lactose
keywords: Staphylococcus aureus; biofilm; lactose; mastitis; milk