title: Comparative proteomic analysis of two tobacco (Nicotiana tabacum) genotypes differing in Cd tolerance.
abstract: Tobacco can easily accumulate cadmium (Cd) in leaves and thus poses a potential threat to human health. Cd-stress-hydroponic-experiments were performed, and the proteomic and transcriptional features of two contrasting tobacco genotypes Yun-yan2 (Cd-tolerant) and Guiyan1 (Cd-sensitive) were compared. We identified 18 Cd-tolerance-associated proteins in leaves, using 2-dimensional gel electrophoresis coupled with mass spectrometry, whose expression were significantly induced in Yunyan2 leaves but down-regulated/unchanged in Guiyan1, or unchanged in Yunyan2 but down-regulated in Guiyan1 under 50 µM Cd stress. They are including epoxide hydrolase, enoyl-acyl-carrier-protein reductase, NPALDP1, chlorophyll a-b binding protein 25, heat shock protein 70 and 14-3-3 proteins. They categorized as 8 groups of their functions: metabolism, photosynthesis, stress response, signal transduction, protein synthesis, protein processing, transport and cell structure. Furthermore, the expression patterns of three Cd-responsive proteins were validated by quantitative real-time PCR. Our findings provide an insight into proteomic basis for Cd-detoxification in tobacco which offers molecular resource for Cd-tolerance.
authors.affiliation: Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Lupeng
authors.initials: L
authors.lastname: Xie
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: He
authors.forename: Shenghua
authors.initials: S
authors.lastname: Shang
authors.forename: Weite
authors.initials: W
authors.lastname: Zheng
authors.forename: Wenxing
authors.initials: W
authors.lastname: Liu
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhang
authors.forename: Feibo
authors.initials: F
authors.lastname: Wu
mesh_terms: D001692:Biological Transport; D002104:Cadmium / Q000633:toxicity*; D004351:Drug Resistance / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D018527:Hydroponics; D010788:Photosynthesis; D010940:Plant Proteins / Q000235:genetics* / Q000502:physiology; D040901:Proteomics; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D012989:Soil Pollutants / Q000633:toxicity*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D012989:Soil Pollutants; D002104:Cadmium