title: In vitro changes in secretion activity of rat ovarian fragments induced by molybdenum.
abstract: The aim of this in vitro study was to examine the secretion activity (progesterone, 17beta-estradiol and insulin-like growth factor-I) of rat ovarian fragments after molybdenum (Mo) addition. Rat ovarian fragments were incubated with ammonium molybdate (NH(4))(6)Mo(7)O(24).4H(2)O at the doses 90, 170, 330 and 500 microg.ml(-1) for 24 h and compared with control group without Mo addition. Release of progesterone (P(4)), estradiol (17beta-estradiol) and insulin-like growth factor I (IGF-I) by ovarian fragments was assessed by radioimmunoassay (RIA). Data show that P(4) release by ovarian fragments was not affected by (NH(4))(6).Mo(7)O(24).4H(2)O addition at all the doses used (90-500 microg.ml(-1)). However, addition of ammonium molybdate was found to cause a significant (P<0.05) dose-dependent decrease (at the doses 90, 170 and 500 microg.ml(-1)) in release of 17beta-estradiol by ovarian fragments in comparison to control. Also, addition of ammonium molybdate significantly (P<0.05) inhibited IGF-I release at all the doses (90-500 microg.ml(-1)) used in the study. Results suggest ammonium molybdate induced inhibition in the release of growth factor IGF-I and its dose-dependent effect on secretion of steroid hormone 17beta-estradiol but not progesterone. These data contribute to new insights regarding the mechanism of action of Mo on rat ovarian functions.
authors.affiliation: Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic. Adriana.Kolesarova@uniag.sk.
authors.forename: S
authors.initials: S
authors.lastname: Roychoudhury
authors.forename: L
authors.initials: L
authors.lastname: Detvanova
authors.forename: A V
authors.initials: AV
authors.lastname: Sirotkin
authors.forename: R
authors.initials: R
authors.lastname: Toman
authors.forename: A
authors.initials: A
authors.lastname: Kolesarova
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008982:Molybdenum / Q000494:pharmacology*; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism; D051381:Rats
chemical_list: D011374:Progesterone; D004958:Estradiol; D007334:Insulin-Like Growth Factor I; D008982:Molybdenum; C022175:ammonium molybdate