title: Simiduia aestuariiviva sp. nov., a gammaproteobacterium isolated from a tidal flat sediment.
abstract: A Gram-stain negative, aerobic, motile and rod-shaped bacterial strain, designated J-MY2(T), was isolated from a tidal flat sediment of the South Sea, South Korea. Strain J-MY2(T) was found to grow optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain J-MY2(T) forms a cluster with the type strains of Simiduia species. Strain J-MY2(T) exhibited 16S rRNA gene sequence similarity values of 97.62-98.77 % to the type strains of four Simiduia species and of <92.95 % sequence similarity to the type strains of the other recognized species. Strain J-MY2(T) was found to contain Q-8 as the predominant ubiquinone and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C16:0, C18:1 ω7c and C17:1 ω8c as the major fatty acids. The major polar lipids of strain J-MY2(T) were identified as phosphatidylethanolamine, phosphatidylglycerol, three unidentified glycolipids and one unidentified lipid. The DNA G+C content of strain J-MY2(T) was determined to be 54.8 mol% and its mean DNA-DNA relatedness values with the type strains of the four Simiduia species were in the range 21-34 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain J-MY2(T) is separated from other Simiduia species. On the basis of the data presented, strain J-MY2(T) is considered to represent a novel species of the genus Simiduia, for which the name Simiduia aestuariiviva sp. nov. is proposed. The type strain is J-MY2(T) ( = KCTC 42073(T) = CECT 8571(T)).
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.forename: Soo-In
authors.initials: SI
authors.lastname: Kim
authors.forename: Chul-Hyung
authors.initials: CH
authors.lastname: Kang
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Park
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D019015:Geologic Sediments / Q000382:microbiology*; D006017:Glycolipids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride