title: Epinephrine evokes renalase secretion via α-adrenoceptor/NF-κB pathways in renal proximal tubular epithelial cells.
abstract: OBJECTIVE
  Renalase is a recently discovered, kidney-specific monoamine oxidase that metabolizes circulating catecholamines. These findings present new insights into hypertension and chronic kidney diseases. Previous data demonstrated that renalase was mainly secreted from proximal tubules which could be evoked by catecholamines. The purpose of this study is to investigate whether renalase expression is induced by epinephrine via α-adrenoceptor/NFκB pathways.
  
  
  METHODS
  HK2 cells were utilized to explore renalase expression in response to epinephrine in vitro. Phentolamine, an α-adrenoceptor antagonist, and Tosyl Phenylalanyl Chloromethyl Ketone (TPCK) were used to block α-adrenoceptor and to knock down the transcription factor NFκB, respectively. Renalase expression was analyzed using Western blot and quantitative PCR.
  
  
  RESULTS
  Both protein and mRNA levels of renalase in HK2 cells increased in response to epinephrine (P<0.05). Epinephrine-evoked renalase expression was attenuated by phentolamine and TPCK separately (P<0.05).
  
  
  CONCLUSIONS
  Epinephrine evokes renalase secretion via α-adrenoceptor/NF-κB pathways in renal proximal tubular epithelial cells.
authors.affiliation: Department of Nephrology and Rheumatology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Hongyan
authors.initials: H
authors.lastname: Cai
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.forename: Tao
authors.initials: T
authors.lastname: Xing
authors.forename: Junhui
authors.initials: J
authors.lastname: Li
authors.forename: Niansong
authors.initials: N
authors.lastname: Wang
mesh_terms: D000316:Adrenergic alpha-Agonists / Q000494:pharmacology*; D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D002460:Cell Line; D004837:Epinephrine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008995:Monoamine Oxidase / Q000378:metabolism*; D016328:NF-kappa B / Q000187:drug effects*; D010646:Phentolamine / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects*; D015398:Signal Transduction / Q000187:drug effects; D014108:Tosylphenylalanyl Chloromethyl Ketone / Q000494:pharmacology
chemical_list: D000316:Adrenergic alpha-Agonists; D000317:Adrenergic alpha-Antagonists; D016328:NF-kappa B; D011500:Protein Synthesis Inhibitors; D011942:Receptors, Adrenergic, alpha; D014108:Tosylphenylalanyl Chloromethyl Ketone; D008995:Monoamine Oxidase; C521557:renalase; D004837:Epinephrine; D010646:Phentolamine