title: mRNA of cytokines in bone marrow and bone biomarkers in response to propranolol in a nutritional growth retardation model.
abstract: BACKGROUND
  The aim of this study was to assess mRNA of IL-6, TNFα and IL-10 cytokines in bone marrow, possible mediators involved in altered bone remodeling with detrimental consequences on bone quality in NGR (Nutritional growth retardation) rats.
  
  
  METHODS
  Weanling male Wistar rats were assigned either to control (C) or experimental group (NGR) (n=20 each). C and NGR groups were assigned to 2 groups according to receiving saline solution (SS) or propranolol hydrochloride (P): C, C+P (CP), NGR or NGR+P (NGRP). For 4 weeks, NGR and NGRP rats received 80% of the amount of food consumed by C and CP, respectively, the previous day, corrected by body weight. P (7 mg/kg/day) was injected ip 5 days/week, for 4 weeks in CP and NGRP rats. Body weight and length were recorded. After 4 weeks, blood was drawn. Femurs were dissected for RNA isolation from bone marrow and mRNA of cytokines assays.
  
  
  RESULTS
  Food restriction induced a significant negative effect on body growth in NGR and NGRP rats (p<0.001). P had no effects on zoometric parameters (p>0.05). CTX-I increased in NGR rats vs. C (p<0.001), but diminished in NGRP (p<0.01). Serum osteocalcin, PTH, calcium and phosphate levels remained unchanged between groups (p>0.05). In NGR, bone marrow IL-6 mRNA and IL-10 mRNA levels were low as compared to other groups (p<0.05). In contrast, bone marrow TNF-α mRNA levels were significantly high (p<0.05).
  
  
  CONCLUSIONS
  This study provides evidences that NGR outcomes in a bone marrow proinflammatory microenvironment leading to unbalanced bone remodeling by enhancement of bone resorption reverted by propranolol.
authors.affiliation: Department of Histology, School of Dentistry, University of Buenos Aires, Buenos Aires, Argentina; School of Science and Technology, National University of San Martin, Buenos Aires, Argentina.
authors.forename: Deborah R
authors.initials: DR
authors.lastname: Tasat
authors.affiliation: Department of Physiology, School of Dentistry, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: Christian E
authors.initials: CE
authors.lastname: Lezón
authors.affiliation: School of Science and Technology, National University of San Martin, Buenos Aires, Argentina.
authors.forename: Francisco
authors.initials: F
authors.lastname: Astort
authors.affiliation: Department of Physiology, School of Dentistry, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Pintos
authors.affiliation: Department of Biochemistry, School of Dentistry, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: Elisa V
authors.initials: EV
authors.lastname: Macri
authors.affiliation: Department of Biochemistry, School of Dentistry, University of Buenos Aires, Buenos Aires, Argentina. Electronic address: friedman@odon.uba.ar.
authors.forename: Silvia M
authors.initials: SM
authors.lastname: Friedman
authors.affiliation: Department of Physiology, School of Dentistry, University of Buenos Aires, Buenos Aires, Argentina. Electronic address: pboyer@fisio.odon.uba.ar.
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Boyer
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism; D016723:Bone Remodeling / Q000187:drug effects*; D004195:Disease Models, Animal; D005269:Femur; D005508:Food Deprivation / Q000502:physiology*; D006130:Growth Disorders / Q000188:drug therapy* / Q000503:physiopathology; D016753:Interleukin-10 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D008297:Male; D011433:Propranolol / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D015415:Biomarkers; D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D011433:Propranolol
keywords: Bone turnover; Cytokines; Nutritional growth retardation; Propranolol