title: Precision-cut luteal slices: a promising approach for studying luteal function in pigs.
abstract: The study was aimed to validate the precision-cut luteal slices to investigate porcine luteal function. Corpora lutea (CLs) were cut into 180-μm thick slices using Krumdick Tissue Slicer. The viability, tissue structure and steroidogenic acute regulatory protein (STAR) expression in the luteal slices did not differ between the beginning and the end of the 24-h incubation period. The luteal progesterone secretion showed a time- and dose-dependent response to porcine luteinizing hormone. The effects of prostaglandin F(2α) and 17β-estradiol on progesterone secretion by porcine luteal slices were comparable to the previously reported in vivo results of the CL microdialysis system in the pig.
authors.affiliation: Department of Hormonal Action Mechanisms, Institute of Animal Reproduction and Food Research of PAS, Bydgoska 7 Str., 10-243 Olsztyn, Poland.
authors.forename: Emilia
authors.initials: E
authors.lastname: Przygrodzka
authors.affiliation: Department of Hormonal Action Mechanisms, Institute of Animal Reproduction and Food Research of PAS, Bydgoska 7 Str., 10-243 Olsztyn, Poland.
authors.forename: Marcelina
authors.initials: M
authors.lastname: Lopinska
authors.affiliation: Department of Hormonal Action Mechanisms, Institute of Animal Reproduction and Food Research of PAS, Bydgoska 7 Str., 10-243 Olsztyn, Poland. Electronic address: a.ziecik@pan.olsztyn.pl.
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Ziecik
mesh_terms: D000818:Animals; D002470:Cell Survival; D003338:Corpus Luteum / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D003433:Crosses, Genetic; D015237:Dinoprost / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D066298:In Vitro Techniques / Q000662:veterinary; D007700:Kinetics; D034461:Luteinization / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism; D034421:Sus scrofa / Q000254:growth & development / Q000502:physiology*; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D010750:Phosphoproteins; D000098682:Steroidogenic Acute Regulatory Protein; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D015237:Dinoprost
keywords: Corpus luteum; Luteal function; Luteolytic sensitivity; Pig; Precision-cut tissue slices model