title: Axonally synthesized ATF4 transmits a neurodegenerative signal across brain regions.
abstract: In Alzheimer's disease (AD) brain, exposure of axons to Aβ causes pathogenic changes that spread retrogradely by unknown mechanisms, affecting the entire neuron. We found that locally applied Aβ1-42 initiates axonal synthesis of a defined set of proteins including the transcription factor ATF4. Inhibition of local translation and retrograde transport or knockdown of axonal Atf4 mRNA abolished Aβ-induced ATF4 transcriptional activity and cell loss. Aβ1-42 injection into the dentate gyrus (DG) of mice caused loss of forebrain neurons whose axons project to the DG. Protein synthesis and Atf4 mRNA were upregulated in these axons, and coinjection of Atf4 siRNA into the DG reduced the effects of Aβ1-42 in the forebrain. ATF4 protein and transcripts were found with greater frequency in axons in the brain of AD patients. These results reveal an active role for intra-axonal translation in neurodegeneration and identify ATF4 as a mediator for the spread of AD pathology.
authors.affiliation: The Taub Institute for Research on Alzheimer's Disease and the Aging Brain, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
authors.forename: Jimena
authors.initials: J
authors.lastname: Baleriola
authors.affiliation: Integrated Program in Cellular, Molecular and Biomedical Studies, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
authors.forename: Chandler A
authors.initials: CA
authors.lastname: Walker
authors.affiliation: Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
authors.forename: Ying Y
authors.initials: YY
authors.lastname: Jean
authors.affiliation: The Taub Institute for Research on Alzheimer's Disease and the Aging Brain, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA; Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
authors.forename: John F
authors.initials: JF
authors.lastname: Crary
authors.affiliation: The Taub Institute for Research on Alzheimer's Disease and the Aging Brain, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA; Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA; Department of Neurology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
authors.forename: Carol M
authors.initials: CM
authors.lastname: Troy
authors.affiliation: The Taub Institute for Research on Alzheimer's Disease and the Aging Brain, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA; Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
authors.forename: Peter L
authors.initials: PL
authors.lastname: Nagy
authors.affiliation: The Taub Institute for Research on Alzheimer's Disease and the Aging Brain, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA; Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA. Electronic address: uh2112@cumc.columbia.edu.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Hengst
mesh_terms: D051701:Activating Transcription Factor 4 / Q000032:analysis* / Q000378:metabolism; D000544:Alzheimer Disease / Q000473:pathology*; D016229:Amyloid beta-Peptides / Q000235:genetics; D000818:Animals; D001369:Axons / Q000378:metabolism; D001921:Brain / Q000166:cytology / Q000473:pathology*; D001923:Brain Chemistry; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism; D006624:Hippocampus; D006801:Humans; D008810:Mice, Inbred C57BL; D051381:Rats; D051742:Transcription Factor CHOP / Q000378:metabolism
chemical_list: C497109:ATF4 protein, human; D016229:Amyloid beta-Peptides; D015852:Eukaryotic Initiation Factor-2; D051701:Activating Transcription Factor 4; D051742:Transcription Factor CHOP