title: Impaired responses to gliadin and gut microbes of immune cells from mice with altered stress-related behavior and premature immune senescence.
abstract: Stress is associated with impaired communication between the nervous and immune systems leading to immunosenescence and increased disease risk. We investigated whether leukocytes from mice with altered stress-related behavior and premature immunosenescence, as well as from chronologically aged mice differently responded ex vivo to celiac disease (CD) triggers (gliadin) and intestinal bacteria by ELISA and flow cytometry and differed in microbiota composition. We found that altered stress-related behavior and premature immunosenescence led to alterations in T lymphocytes and cytokine release of immune cells basally and in response to peptic fragments of gliadin and commensal and pathogenic bacteria, possibly increasing susceptibility to CD in adulthood.
authors.affiliation: Microbial Ecology, Nutrition & Health Research Group, Institute of Agrochemistry and Food Technology, National Research Council (IATA-CSIC), Valencia, Spain.
authors.forename: G
authors.initials: G
authors.lastname: De Palma
authors.affiliation: Department of Physiology, Faculty of Biology, Complutense University of Madrid, Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Vida
authors.affiliation: Microbial Ecology, Nutrition & Health Research Group, Institute of Agrochemistry and Food Technology, National Research Council (IATA-CSIC), Valencia, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Santacruz
authors.affiliation: Department of Physiology, Faculty of Biology, Complutense University of Madrid, Madrid, Spain.
authors.forename: N M
authors.initials: NM
authors.lastname: De Castro
authors.affiliation: Department of Physiology, Faculty of Biology, Complutense University of Madrid, Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: De la Fuente
authors.affiliation: Microbial Ecology, Nutrition & Health Research Group, Institute of Agrochemistry and Food Technology, National Research Council (IATA-CSIC), Valencia, Spain. Electronic address: yolsanz@iata.csic.es.
authors.forename: Y
authors.initials: Y
authors.lastname: Sanz
mesh_terms: D000375:Aging / Q000276:immunology*; D000704:Analysis of Variance; D000818:Animals; D039941:Bifidobacteriales Infections / Q000276:immunology / Q000473:pathology*; D002446:Celiac Disease / Q000139:chemically induced* / Q000276:immunology; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D005903:Gliadin / Q000009:adverse effects*; D051379:Mice; D008813:Mice, Inbred ICR; D013315:Stress, Psychological / Q000473:pathology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D016207:Cytokines; D005903:Gliadin
keywords: Aging; Celiac disease; Intestinal bacteria; Leukocytes; Stress-related behavior