title: Nanoscale label-free bioprobes to detect intracellular proteins in single living cells.
abstract: Fluorescent labeling techniques have been widely used in live cell studies; however, the labeling processes can be laborious and challenging for use in non-transfectable cells, and labels can interfere with protein functions. While label-free biosensors have been realized by nanofabrication, a method to track intracellular protein dynamics in real-time, in situ and in living cells has not been found. Here we present the first demonstration of label-free detection of intracellular p53 protein dynamics through a nanoscale surface plasmon-polariton fiber-tip-probe (FTP).
authors.affiliation: 1] Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA [2] Rowland Institute at Harvard University, Cambridge, MA 02142, USA.
authors.forename: Wooyoung
authors.initials: W
authors.lastname: Hong
authors.affiliation: Rowland Institute at Harvard University, Cambridge, MA 02142, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Liang
authors.affiliation: Rowland Institute at Harvard University, Cambridge, MA 02142, USA.
authors.forename: Diane
authors.initials: D
authors.lastname: Schaak
authors.affiliation: School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Marko
authors.initials: M
authors.lastname: Loncar
authors.affiliation: Rowland Institute at Harvard University, Cambridge, MA 02142, USA.
authors.forename: Qimin
authors.initials: Q
authors.lastname: Quan
mesh_terms: D006046:Gold / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D043942:Nanotubes / Q000737:chemistry*; D059010:Single-Cell Analysis / Q000295:instrumentation*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D006046:Gold