title: Elevated levels of FMR1 mRNA in granulosa cells are associated with low ovarian reserve in FMR1 premutation carriers.
abstract: OBJECTIVE
  To assess the role of mRNA accumulation in granulosa cells as the cause of low ovarian response among FMR1 premutation carriers undergoing pre-implantation genetic diagnosis (PGD).
  
  
  METHODS
  Case control study in an academic IVF unit. Twenty-one consecutive FMR1 premutation carriers and 15 control women were included. After oocyte retrieval the granulosa cells mRNA levels of FMR1 was measured using RT-PCR.
  
  
  RESULTS
  In FMR1 premutation carriers, there was a significant non-linear association between the number of CGG repeats and the number of retrieved oocytes (p<0.0001) and a trend to granulosa cells FMR1 mRNA levels (p = 0.07). The lowest number of retrieved oocytes and the highest level of mRNA were seen in women with mid-size CGG repeats (80-120). A significant negative linear correlation was observed between the granulosa cells FMR1 mRNA levels and the number of retrieved oocytes (R2 linear = 0.231, P = 0.02).
  
  
  CONCLUSIONS
  We suggest that there is a no-linear association between the number of CGG repeats and ovarian function, resulting from an increased granulosa cells FMR1 mRNA accumulation in FMR1 carriers in the mid-range (80-120 repeats).
authors.affiliation: Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, (Tel Hashomer), Ramat Gan, Israel, and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Shai E
authors.initials: SE
authors.lastname: Elizur
authors.affiliation: Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, (Tel Hashomer), Ramat Gan, Israel, and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Oshrit
authors.initials: O
authors.lastname: Lebovitz
authors.affiliation: Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, (Tel Hashomer), Ramat Gan, Israel, and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Sanaz
authors.initials: S
authors.lastname: Derech-Haim
authors.affiliation: Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, (Tel Hashomer), Ramat Gan, Israel, and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Olga
authors.initials: O
authors.lastname: Dratviman-Storobinsky
authors.affiliation: Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, (Tel Hashomer), Ramat Gan, Israel, and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Baruch
authors.initials: B
authors.lastname: Feldman
authors.affiliation: Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, (Tel Hashomer), Ramat Gan, Israel, and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Jehoshua
authors.initials: J
authors.lastname: Dor
authors.affiliation: Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, (Tel Hashomer), Ramat Gan, Israel, and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Raoul
authors.initials: R
authors.lastname: Orvieto
authors.affiliation: Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, (Tel Hashomer), Ramat Gan, Israel, and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Yoram
authors.initials: Y
authors.lastname: Cohen
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D006107:Granulosa Cells / Q000378:metabolism*; D006579:Heterozygote; D006801:Humans; D054315:Oocyte Retrieval; D065851:Ovarian Reserve / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D051860:Fragile X Messenger Ribonucleoprotein 1; D012333:RNA, Messenger; C497683:FMR1 protein, human