title: Tumor necrosis factor beta NcoI polymorphism is associated with inflammatory and metabolic markers in multiple sclerosis patients.
abstract: To evaluate the association between the tumor necrosis factor beta (TNF-β) NcoI polymorphism and inflammatory and metabolic markers in patients with multiple sclerosis (MS) patients and the association of these markers with disease disability, a 782 base-pair fragment of the TNF-β gene was amplified from genomic DNA and digested with the NcoI restriction enzyme. The serum levels of numerous cytokines (IL-1β, IL-12, IL-6, TNF-α, IFN-γ, IL-4, IL-10, and IL-17) serum lipid levels, plasma insulin levels, and the Homeostasis Model Assessment-Insulin Resistance (HOMA-IR) levels were evaluated in 123 female and 43 male patients with MS. Females carrying the TNFB2/B2 genotype presented with decreased IL-4 and IL-10 levels and increased TNF-α, glucose, insulin, and HOMA-IR levels; moreover, there were positive correlations between EDSS and glucose and between EDSS and HOMA-IR in these females. Males carrying the TNFB2/B2 genotype exhibited increased levels of TNF-α, IFN-γ, and IL-17 (p=0.0326) and decreased levels of IL-4, IL-10, insulin, and HOMA-IR; there was a positive correlation between EDSS and TNF-α levels. The TNFB2/B2 genotype of TNF-β NcoI polymorphism was associated with increased inflammatory and metabolic markers and this association was different according to sex of MS patients.
authors.affiliation: Health Sciences Postgraduate Program, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Kallaur
authors.affiliation: Health Sciences Postgraduate Program, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil.
authors.forename: Sayonara Rangel
authors.initials: SR
authors.lastname: Oliveira
authors.affiliation: Department of Pathology, Clinical Analysis and Toxicology, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil.
authors.forename: Andréa Name Colado
authors.initials: AN
authors.lastname: Simão
authors.affiliation: Department of Pathology, Clinical Analysis and Toxicology, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil.
authors.forename: Elaine Regina Delicato
authors.initials: ER
authors.lastname: de Almeida
authors.affiliation: Department of Pathology, Clinical Analysis and Toxicology, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil.
authors.forename: Helena Kaminami
authors.initials: HK
authors.lastname: Morimoto
authors.affiliation: Health Sciences Postgraduate Program, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil.
authors.forename: Daniela Frizon
authors.initials: DF
authors.lastname: Alfieri
authors.affiliation: Health Sciences Postgraduate Program, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil; Outpatient Clinic for Multiple Sclerosis, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil.
authors.forename: Wildea Lice de Carvalho Jennings
authors.initials: WL
authors.lastname: Pereira
authors.affiliation: Department of Clinical Analysis, Laboratory of Immunogenetics, State University of Maringá, Maringá, Paraná, Brazil.
authors.forename: Sueli Donizete
authors.initials: SD
authors.lastname: Borelli
authors.affiliation: Outpatient Clinic for Multiple Sclerosis, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil; Department of Clinical Medicine, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil.
authors.forename: Damácio Ramon
authors.initials: DR
authors.lastname: Kaimen-Maciel
authors.affiliation: Impact Strategic Research Centre, School of Medicine, Deakin University, Geelong, Victoria, Australia; Department of Psychiatry, King Chulalongorn Memorial Hospital, Chulalongkorn, Bangkok, Thailand.
authors.forename: Michael
authors.initials: M
authors.lastname: Maes
authors.affiliation: Department of Pathology, Clinical Analysis and Toxicology, Health Sciences Center, State University of Londrina, Londrina, Paraná, Brazil. Electronic address: reiche@sercomtel.com.br.
authors.forename: Edna Maria Vissoci
authors.initials: EM
authors.lastname: Reiche
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood*; D001786:Blood Glucose; D016207:Cytokines / Q000097:blood*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000235:genetics; D008233:Lymphotoxin-alpha / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood* / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D055815:Young Adult
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D016207:Cytokines; D007328:Insulin; D008233:Lymphotoxin-alpha; D014409:Tumor Necrosis Factor-alpha; C052380:endodeoxyribonuclease NcoI; D015252:Deoxyribonucleases, Type II Site-Specific
keywords: Genetic polymorphism; Inflammatory markers; Insulin resistance; Metabolic markers; Multiple sclerosis; Tumor necrosis factor