title: Redox and catalysis 'all-in-one' infinite coordination polymer for electrochemical immunosensor of tumor markers.
abstract: Prostate-specific antigen (PSA), as a glycoprotein enzyme encoded in humans by the KLK3 gene, is one of the most important biomarkers for the diagnosis and prognosis of prostate cancer. Herein, a new electrochemical immunosensor for sensitive determination of PSA was designed by using redox and catalysis 'all-in-one' infinite coordination polymer (PtNP@ICP) as signal tag on the polyamidoamine dendrimers modified electrode interface. To construct such 'all-in-one' PtNP@ICP nanostructures, the coordination polymerization was fully carried between metal ions and polydentate bridging ligands, and the PtNP was encapsulated into the ICP in the process of polymerization. The prepared PtNP@ICP nanocatalyst was characterized by transmission electron microscope (TEM), energy dispersive X-ray spectrometry (EDX), ultraviolet and visible (UV-vis) spectrophotometry and Fourier transform infrared spectroscope (FTIR). And the synthesized PtNP@ICP was utilized as signal tag for the label of PSA. With a sandwich-type immunoassay format, the conjugated signal tag on the transducer increased with the increasing PSA concentration in the sample thus enhancing the signal of the electrochemical immunosensor due to the catalytic reduction toward H2O2 of the enveloped PtNP. Under optimal conditions, the current was proportional to the logarithm of PSA concentration ranging from 0.001 to 60 ng/mL. The detection limit (LOD) was 0.3 pg/mL at 3 sB. The immunosensor displayed an acceptable reproducibility, stability and selectivity. In addition, the methodology was evaluated with human serum specimens receiving good correlation with results from commercialized enzyme-linked immunosorbent assay (ELISA) method.
authors.affiliation: Institute of Nanomedicine and Nanobiosensing, Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Institute of Nanomedicine and Nanobiosensing, Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
authors.forename: Bingqian
authors.initials: B
authors.lastname: Liu
authors.affiliation: Institute of Nanomedicine and Nanobiosensing, Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
authors.affiliation: Institute of Nanomedicine and Nanobiosensing, Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China. Electronic address: dianping.tang@fzu.edu.cn.
authors.forename: Dianping
authors.initials: D
authors.lastname: Tang
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood; D015374:Biosensing Techniques / Q000379:methods; D050091:Dendrimers / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010984:Platinum / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D017430:Prostate-Specific Antigen / Q000097:blood*; D015203:Reproducibility of Results
chemical_list: D014408:Biomarkers, Tumor; D050091:Dendrimers; C531249:Poly(amidoamine); D011073:Polyamines; D011108:Polymers; D010984:Platinum; D017430:Prostate-Specific Antigen
keywords: 'All-in-one'; Catalysis; Electrochemical immunosensor; Infinite coordination polymerization; Prostate-specific antigen; Redox