title: Seeing diabetes: visual detection of glucose based on the intrinsic peroxidase-like activity of MoS2 nanosheets.
abstract: Molybdenum disulfide (MoS2) has attracted increasing research interest recently due to its unique physical, optical and electrical properties, correlated with its 2D ultrathin atomic-layered structure. Until now, however, great efforts have focused on its applications such as lithium ion batteries, transistors, and hydrogen evolution reactions. Herein, for the first time, MoS2 nanosheets are discovered to possess an intrinsic peroxidase-like activity and can catalytically oxidize 3,3',5,5'-tetramethylbenzidine (TMB) by H2O2 to produce a color reaction. The catalytic activity follows the typical Michaelis-Menten kinetics and is dependent on temperature, pH, H2O2 concentration, and reaction time. Based on this finding, a highly sensitive and selective colorimetric method for H2O2 and glucose detection is developed and applied to detect glucose in serum samples. Moreover, a simple, inexpensive, instrument-free and portable test kit for the visual detection of glucose in normal and diabetic serum samples is constructed by utilizing agarose hydrogel as a visual detection platform.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for food safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety Department of Chemistry, Fuzhou University, Fuzhou, 350116, China. lqguo@fzu.edu.cn.
authors.forename: Tianran
authors.initials: T
authors.lastname: Lin
authors.forename: Liangshuang
authors.initials: L
authors.lastname: Zhong
authors.forename: Liangqia
authors.initials: L
authors.lastname: Guo
authors.forename: Fengfu
authors.initials: F
authors.lastname: Fu
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D001560:Benzidines / Q000737:chemistry; D001786:Blood Glucose / Q000032:analysis*; D003124:Colorimetry; D003920:Diabetes Mellitus / Q000175:diagnosis*; D004220:Disulfides / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D046529:Microscopy, Electron, Transmission; D008982:Molybdenum / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology; D009195:Peroxidase / Q000378:metabolism; D012685:Sepharose / Q000737:chemistry; D013696:Temperature
chemical_list: D001560:Benzidines; D001786:Blood Glucose; D004220:Disulfides; D020100:Hydrogels; C021758:3,3',5,5'-tetramethylbenzidine; D008982:Molybdenum; D012685:Sepharose; D006861:Hydrogen Peroxide; D009195:Peroxidase; C082964:molybdenum disulfide