title: [Mechanism research of aromatics borneol and muscone].
abstract: OBJECTIVE
  To study the effects of borneol and muscone on membrane fluidity, membrane potential, Na+, K(+)-ATPase activity and calcium ions of blood brain barrier (BBB) model cells (MDCK and MDCK-MDR1) for exploring the mechanism of aromatics.
  
  
  METHODS
  MDCK and MDCK-MDR1 cells were incubated and the experiment was performed as following. Cells were incubated with aromatic herbs for 3 h. The membrane fluidity were detected by FRAP. The changes of membrane potentials and the concentration of calcium ions were detected by flow cytometer.
  
  
  RESULTS
  Borneol (55.6, 111.2 microg/mL) and muscone (8.34, 16.68 microg/mL) significantly enhanced the cell membrane fluidity. Borneol (27.8, 55.6, 111.2 microg/mL) and muscone (4.17, 8.34, 16.68 microg/mL) made the MDCK and MDCK-MDR1 membrane potentials less negative or depolarized. Borneol increased the concentration of intracellular free calcium in MDCK while decreased the concentration of intracellular free calcium in MDCK-MDR1 cells. Muscone increased the concentration of calcium in MDCK and MDCK-MDR1 cells. Na+, K(+)-ATPase activity was significantly increased in borneol and muscone group.
  
  
  CONCLUSIONS
  The regulating effect of membrane fluidity, membrane potential, Na+, K(+)-ATPase activity and calcium ions in MDCK and MDCK-MDR1 cells might be one of the mechanisms of borneol and muscone's BBB opening function.
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Chen
authors.forename: Shou-Ying
authors.initials: SY
authors.lastname: Du
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.forename: Pan
authors.initials: P
authors.lastname: Xu
authors.forename: Bing
authors.initials: B
authors.lastname: Yang
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Liu
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D001892:Camphanes / Q000008:administration & dosage / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D002463:Cell Membrane Permeability; D003516:Cycloparaffins / Q000008:administration & dosage / Q000494:pharmacology*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D039823:Iridoids / Q000008:administration & dosage / Q000494:pharmacology; D061985:Madin Darby Canine Kidney Cells; D008564:Membrane Potentials / Q000187:drug effects* / Q000502:physiology; D008954:Models, Biological; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D001892:Camphanes; D003516:Cycloparaffins; D039823:Iridoids; C007835:geniposide; D000254:Sodium-Potassium-Exchanging ATPase; C022871:isoborneol; D002118:Calcium; C031021:muscone