title: Advances in yeast genome engineering.
abstract: Genome engineering based on homologous recombination has been applied to yeast for many years. However, the growing importance of yeast as a cell factory in metabolic engineering and chassis in synthetic biology demands methods for fast and efficient introduction of multiple targeted changes such as gene knockouts and introduction of multistep metabolic pathways. In this review, we summarize recent improvements of existing genome engineering methods, the development of novel techniques, for example for advanced genome redesign and evolution, and the importance of endonucleases as genome engineering tools.
authors.affiliation: Department of Chemical and Biological Engineering, Chalmers University of Technology, Göteborg, Sweden.
authors.forename: Florian
authors.initials: F
authors.lastname: David
authors.affiliation: Department of Chemical and Biological Engineering, Chalmers University of Technology, Göteborg, Sweden siewers@chalmers.se.
authors.forename: Verena
authors.initials: V
authors.lastname: Siewers
mesh_terms: D004720:Endonucleases / Q000378:metabolism; D000072669:Gene Editing; D055786:Gene Knockout Techniques; D005818:Genetic Engineering; D059765:Homologous Recombination; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D058615:Synthetic Biology
chemical_list: D004720:Endonucleases
keywords: Genome editing; Sc2.0; adaptive laboratory evolution; endonucleases