title: Analysis of androgen and anti-androgen regulation of KLK-related peptidase 2, 3, and 4 alternative transcripts in prostate cancer.
abstract: We assessed whether alternative transcripts (using KLK2, KLK3 and KLK4 as models) are differentially regulated by androgens and anti-androgens as an indicator of prostate cancers as they acquire treatment resistance. Using RNAseq of LNCaP cells treated with dihydrotestosterone, bicalutamide and enzalutamide, we show that the expression of variant KLK transcripts is markedly different to other variant transcripts at those loci. We also reveal that KLK variants are also over 2-fold more highly expressed in prostate cancers compared to their corresponding normal prostate. We propose that androgens and anti-androgens can activate specific variant transcripts of critical prostate cancer genes during treatment resistance.
authors.forename: John
authors.initials: J
authors.lastname: Lai
authors.forename: Jiyuan
authors.initials: J
authors.lastname: An
authors.forename: Colleen C
authors.initials: CC
authors.lastname: Nelson
authors.forename: Melanie L
authors.initials: ML
authors.lastname: Lehman
authors.forename: Jyotsna
authors.initials: J
authors.lastname: Batra
authors.forename: Judith A
authors.initials: JA
authors.lastname: Clements
mesh_terms: D017398:Alternative Splicing / Q000187:drug effects / Q000235:genetics*; D000726:Androgen Antagonists / Q000494:pharmacology*; D000728:Androgens / Q000494:pharmacology*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D007610:Kallikreins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D017430:Prostate-Specific Antigen / Q000235:genetics / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D000726:Androgen Antagonists; D000728:Androgens; D012333:RNA, Messenger; C000595667:KLK2 protein, human; C549870:KLK3 protein, human; D007610:Kallikreins; C120801:kallikrein 4; D017430:Prostate-Specific Antigen