title: Ratiometric fluorescent probe based on gold nanoclusters and alizarin red-boronic acid for monitoring glucose in brain microdialysate.
abstract: Glucose monitoring with high sensitivity and accuracy in the cerebrospinal fluid is a challenge for evaluating the role of glucose in the physiological and pathological processes. In this work, a ratiometric fluorescent probe for sensing glucose was developed. In the probe, the gold nanoclusters protected by ovalbumin played the role as the reference of fluorophore and the Alizarin Red S-3-aminophenyl boronic acid immobilized on the poly(N-acryloxysuccinimide) acted as both the response signal and specific recognition unit for sensing glucose. Once the ratiometric fluorescent probe reacted with glucose in the biological system, its fluorescence intensity at 567 nm was quenched, while the fluorescence intensity at 610 nm was essentially unchanged. In addition, the prepared ratiometric fluorescent probe showed higher stability against environmental effects. As a result, the present ratiometric fluorescent probe was successfully used for monitoring of glucose in the rat brain following the cerebral calm/ischemia.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , No. 2 Zhongguancun Beiyijie, Beijing 100190, P. R. China.
authors.forename: Lu-Liang
authors.initials: LL
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Qiao
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Hao
authors.forename: Li
authors.initials: L
authors.lastname: Qi
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Zhou
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Zong-Xiu
authors.initials: ZX
authors.lastname: Nie
authors.forename: Lan-Qun
authors.initials: LQ
authors.lastname: Mao
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000737:chemistry*; D015374:Biosensing Techniques; D001897:Boronic Acids / Q000737:chemistry*; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002545:Brain Ischemia / Q000378:metabolism / Q000473:pathology; D015314:Dialysis Solutions / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D008297:Male; D017551:Microdialysis; D015335:Molecular Probes / Q000737:chemistry*; D049329:Nanostructures; D051381:Rats; D017207:Rats, Sprague-Dawley; D013050:Spectrometry, Fluorescence; D013388:Succinimides / Q000737:chemistry
chemical_list: D000880:Anthraquinones; D001897:Boronic Acids; D015314:Dialysis Solutions; D005456:Fluorescent Dyes; D015335:Molecular Probes; D013388:Succinimides; C028592:3-aminobenzeneboronic acid; C004468:Alizarin Red S; D005947:Glucose