title: Persistent effectivity of gas plasma-treated, long time-stored liquid on epithelial cell adhesion capacity and membrane morphology.
abstract: Research in plasma medicine includes a major interest in understanding gas plasma-cell interactions. The immediate application of gas plasma in vitro inhibits cell attachment, vitality and cell-cell contacts via the liquid. Interestingly, in our novel experiments described here we found that the liquid-mediated plasma effect is long-lasting after storage up to seven days; i. e. the liquid preserves the characteristics once induced by the argon plasma. Therefore, the complete Dulbecco's Modified Eagle cell culture medium was argon plasma-treated (atmospheric pressure, kINPen09) for 60 s, stored for several days (1, 4 and 7 d) at 37°C and added to a confluent mouse hepatocyte epithelial cell (mHepR1) monolayer. Impaired tight junction architecture as well as shortened microvilli on the cell membrane could be observed, which was accompanied by the loss of cell adhesion capacity. Online-monitoring of vital cells revealed a reduced cell respiration. Our first time-dependent analysis of plasma-treated medium revealed that temperature, hydrogen peroxide production, pH and oxygen content can be excluded as initiators of cell physiological and morphological changes. The here observed persisting biological effects in plasma-treated liquids could open new medical applications in dentistry and orthopaedics.
authors.affiliation: Department of Cell Biology, University Medical Center Rostock, Rostock, Germany.
authors.forename: Maxi
authors.initials: M
authors.lastname: Hoentsch
authors.affiliation: Leibniz-Institute for Plasma Science and Technology e.V., Greifswald, Germany.
authors.forename: René
authors.initials: R
authors.lastname: Bussiahn
authors.affiliation: Department of Cell Biology, University Medical Center Rostock, Rostock, Germany.
authors.forename: Henrike
authors.initials: H
authors.lastname: Rebl
authors.affiliation: Department of Cell Biology, University Medical Center Rostock, Rostock, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Bergemann
authors.affiliation: Center for Extracorporeal Organ Support, Department of Internal Medicine, University Medical Center Rostock, Rostock, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Eggert
authors.affiliation: Medical Biology and Electron Microscopic Center, University Medical Center Rostock, Rostock, Germany.
authors.forename: Marcus
authors.initials: M
authors.lastname: Frank
authors.affiliation: Leibniz-Institute for Plasma Science and Technology e.V., Greifswald, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: von Woedtke
authors.affiliation: Department of Cell Biology, University Medical Center Rostock, Rostock, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Nebe
mesh_terms: D000818:Animals; D001128:Argon / Q000378:metabolism*; D002448:Cell Adhesion; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure*; D002470:Cell Survival; D003470:Culture Media / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D005740:Gases / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D008871:Microvilli / Q000378:metabolism / Q000648:ultrastructure; D011309:Preservation, Biological / Q000379:methods; D019108:Tight Junctions / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D003470:Culture Media; D005740:Gases; D001128:Argon