title: P53 nuclear stabilization is associated with FHIT loss and younger age of onset in squamous cell carcinoma of oral tongue.
abstract: BACKGROUND
  Squamous cell carcinoma of tongue (SCCT) is expected to harbor unique clinico-pathological and molecular genetic features since a significant proportion of patients are young and exhibit no association with tobacco or alcohol.
  
  
  METHODS
  We determined P53, epidermal growth factor receptor, microsatellite instability, human papilloma virus infection and loss of heterozygosity status at several tumor suppressor loci in one hundred and twenty one oral SCCT (SSCOT) samples and analyzed their association with clinico-pathological features and patient survival.
  
  
  RESULTS
  Our results revealed a significantly higher incidence of p53 nuclear stabilization in early (as against late) onset SCCOT. FHIT loss was significantly associated with p53 nuclear stabilization and the association was stronger in patients with no history of tobacco use. Samples harboring mutation in p53 DNA binding domain or exhibiting p53 nuclear stabilization, were significantly associated with poor survival.
  
  
  CONCLUSIONS
  Our study has therefore identified distinct features in SCCOT tumorigenesis with respect to age and tobacco exposure and revealed possible prognostic utility of p53.
authors.affiliation: Laboratory of Molecular Oncology, Centre for DNA Fingerprinting and Diagnostics, Nampally, Hyderabad 500001, India.
authors.forename: Raju
authors.initials: R
authors.lastname: Adduri
authors.affiliation: Laboratory of Molecular Oncology, Centre for DNA Fingerprinting and Diagnostics, Nampally, Hyderabad 500001, India.
authors.forename: Viswakalyan
authors.initials: V
authors.lastname: Kotapalli
authors.affiliation: Laboratory of Molecular Oncology, Centre for DNA Fingerprinting and Diagnostics, Nampally, Hyderabad 500001, India ; Currently at National Centre for Cell Science, Ganeshkhind, Pune, India.
authors.forename: Neha A
authors.initials: NA
authors.lastname: Gupta
authors.affiliation: Apollo Hospitals, Jubilee Hills, Hyderabad India.
authors.forename: Swarnalata
authors.initials: S
authors.lastname: Gowrishankar
authors.affiliation: MNJ Institute of Oncology & Regional Cancer Centre, Red Hills, Hyderabad India.
authors.forename: Mukta
authors.initials: M
authors.lastname: Srinivasulu
authors.affiliation: MNJ Institute of Oncology & Regional Cancer Centre, Red Hills, Hyderabad India.
authors.forename: Mohammed Mujtaba
authors.initials: MM
authors.lastname: Ali
authors.affiliation: MNJ Institute of Oncology & Regional Cancer Centre, Red Hills, Hyderabad India ; Currently at Basavatarakam Indo American Cancer Hospital & Research Institute, Hyderabad, India.
authors.forename: Subramanyeshwar
authors.initials: S
authors.lastname: Rao
authors.affiliation: Nizam's Institute of Medical Sciences, Punjagutta, Hyderabad India.
authors.forename: Shantveer G
authors.initials: SG
authors.lastname: Uppin
authors.affiliation: Apollo Hospitals, Jubilee Hills, Hyderabad India.
authors.forename: Umanath K
authors.initials: UK
authors.lastname: Nayak
authors.affiliation: Omega Hospitals, Jubilee Hills, Hyderabad, India.
authors.forename: Snehalatha
authors.initials: S
authors.lastname: Dhagam
authors.affiliation: Omega Hospitals, Jubilee Hills, Hyderabad, India.
authors.forename: Mohana Vamsy
authors.initials: MV
authors.lastname: Chigurupati
authors.affiliation: Laboratory of Molecular Oncology, Centre for DNA Fingerprinting and Diagnostics, Nampally, Hyderabad 500001, India.
authors.forename: Murali Dharan
authors.initials: MD
authors.lastname: Bashyam
keywords: Disease specific survival; EGFR; FHIT; Oral tongue cancer; TP53