title: Immunological features of LPS from Ochrobactrum intermedium on sheep experimentally infected with Fasciola hepatica.
abstract: The effects of the lipopolysaccharide (LPS) from Ochrobactrum intermedium in sheep with fasciolosis was reported previously, resulting in lower fecal egg counts and fluke burden. In the current study, we analyzed its immunological effects in two groups of sheep, treated (T) and controls (C). Fasciolosis induces a T helper (Th) type-2 response, characterized by IL-4 and IL-10 production; however, at the beginning of the infection, the IFN-γ production predominates (Th type-1 response). Although we did not find differences in IL-4 production or in the expression level of this gene in the hepatic lymph nodes, the expression level of IL-10 was higher (P < 0.05) in the T group at 4 wpi. The IFN-γ production was higher (P < 0.01) at 12 wpi as well as its level of expression at 4 wpi (P < 0.05) in the T group. We found a higher expression level of TGF-β at 4 wpi in the T group (P < 0.05), associated with the previous report of thicker fibrous tracks in a treated group. Immunoglobulin G1, related with a Th type-2 response, was higher (P < 0.01) in the T group at 4 and 12 wpi. In conclusion, the effects of LPS from O. intermedium could have resulted from a predominant Th type-2 immune response.
authors.affiliation: Departamento de Sanidad Animal, Parasitología y Enfermedades Parasitarias, Facultad de Veterinaria, Universidad de León, 24071 León, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Martínez-Pérez
authors.affiliation: Departamento de Sanidad Animal, Parasitología y Enfermedades Parasitarias, Facultad de Veterinaria, Universidad de León, 24071 León, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Robles-Pérez
authors.affiliation: Departamento de Sanidad Animal, Parasitología y Enfermedades Parasitarias, Facultad de Veterinaria, Universidad de León, 24071 León, Spain; Instituto de Ganadería de Montaña (CSIC-ULE), Finca de Marzanas, 24346 Grulleros (León), Spain.
authors.forename: F A
authors.initials: FA
authors.lastname: Rojo-Vázquez
authors.affiliation: Instituto de Ganadería de Montaña (CSIC-ULE), Finca de Marzanas, 24346 Grulleros (León), Spain. Electronic address: mmarva@unileon.es.
authors.forename: M
authors.initials: M
authors.lastname: Martínez-Valladares
mesh_terms: D000818:Animals; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D007074:Immunoglobulin G / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism / Q000494:pharmacology*; D042041:Ochrobactrum / Q000378:metabolism*; D012756:Sheep / Q000276:immunology* / Q000469:parasitology*; D012757:Sheep Diseases / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D018418:Th2 Cells / Q000187:drug effects / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma
keywords: Cytokines; Fasciola hepatica; IgG1; Lipopolysaccharide; Ochrobactrum intermedium; Sheep