title: Nuclear fast red as highly sensitive "off/on" fluorescent probe for detecting guanine.
abstract: A novel fluorescence method for the determination of guanine was developed based on the fluorescence enhancement of Cu(2+)-nuclear fast red complex in the presence of guanine in Tris-HCl buffer. The complex of Cu(2+) with nuclear fast red resulted in a dramatic quenching of the fluorescence intensity. Nuclear fast red were dissociated from the complex with the addition of guanine due to the strong interaction between guanine and Cu(2+), which caused the fluorescence enhancement. The enhanced fluorescence intensity was well proportional to the concentration of guanine in the range of 4.96 × 10(-8)-1.09 × 10(-6)mol/L with the limit of detection 1.9 × 10(-8)mol/L. The method has been applied successfully to the determination of guanine in serum and DNA samples, and the recoveries were from 96.0% to 104.8%.
authors.affiliation: School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Chemistry, Xinxiang Medical University, Xinxiang 453007, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.affiliation: School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China.
authors.forename: Suling
authors.initials: S
authors.lastname: Feng
mesh_terms: D000880:Anthraquinones / Q000737:chemistry*; D003300:Copper / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes; D006147:Guanine / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D044967:Serum / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D000880:Anthraquinones; D005456:Fluorescent Dyes; C523186:nuclear fast red; D006147:Guanine; D003300:Copper; D004247:DNA
keywords: Cu(2+); Fluorescence; Guanine; Nuclear fast red