title: Protective effects of epigallocatechin gallate (EGCG) on streptozotocin-induced diabetic nephropathy in mice.
abstract: There is increasing evidence suggesting that antioxidants in green tea extracts may protect kidneys on the progression of end-stage renal disease. We investigated the protective impacts of (-)-epigallocatechin 3-O-gallate (EGCG) against streptozotocin (STZ)-induced diabetic nephropathy in mice. The mice were divided into 5 groups (n=10 per group): control (saline, i.p.), STZ (200mg/kg, i.p.), EGCG50 (50mg/kg, S.Q.), EGCG100 (100mg/kg, S.Q.), and EGCG200 (200mg/kg, S.Q.). Animals were sacrificed at scheduled times after EGCG administration and then quantitative and qualitative analysis were performed. Compared with the control group, the STZ group showed an increase in levels of blood glucose, blood urea nitrogen, creatinine and urine protein amounts with a decrease in body weight. All the above parameters were significantly reversed with EGCG treatment, especially in the EGCG100 group. After STZ injection, there was a mesangial proliferation with increased renal osteopontin accumulation and its protein expression in the glomeruli and the proximal tubules. Mice kidneys after EGCG-treatment showed a reduced expression of above parameters and relatively improved histopathological findings. These results indicated that EGCG 100mg/kg might provide an effective protection against STZ-induced diabetic nephropathy in mice by osteopontin suppression.
authors.affiliation: Department of Anatomy, School of Medicine, Jeju National University, Jeju-Do, Republic of Korea.
authors.forename: Sang Pil
authors.initials: SP
authors.lastname: Yoon
authors.affiliation: Department of Pathology, School of Medicine, Jeju National University, Jeju-Do, Republic of Korea.
authors.forename: Young Hee
authors.initials: YH
authors.lastname: Maeng
authors.affiliation: Department of Pathology, College of Medicine, Chosun University, Gwangju, Republic of Korea.
authors.forename: Ran
authors.initials: R
authors.lastname: Hong
authors.affiliation: Department of Biochemistry, College of Medicine, Chosun University, Gwangju, Republic of Korea.
authors.forename: Byung Rai
authors.initials: BR
authors.lastname: Lee
authors.affiliation: Department of Neurosurgery, College of Medicine, Chosun University, Gwangju, Republic of Korea.
authors.forename: Chong Gue
authors.initials: CG
authors.lastname: Kim
authors.affiliation: Division of Nephrology, Department of Internal Medicine, College of Medicine, Chosun University, Gwangju, Republic of Korea.
authors.forename: Hyun Lee
authors.initials: HL
authors.lastname: Kim
authors.affiliation: Division of Nephrology, Department of Internal Medicine, College of Medicine, Chosun University, Gwangju, Republic of Korea.
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Chung
authors.affiliation: Division of Nephrology, Department of Internal Medicine, College of Medicine, Chosun University, Gwangju, Republic of Korea. Electronic address: bcshin@chosun.ac.kr.
authors.forename: Byung Chul
authors.initials: BC
authors.lastname: Shin
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D003928:Diabetic Nephropathies / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011897:Random Allocation; D013311:Streptozocin / Q000494:pharmacology
chemical_list: D013311:Streptozocin; D002392:Catechin; C045651:epigallocatechin gallate
keywords: Diabetic nephropathy; Epigallocatechin gallate; Mice; Osteopontin; Streptozotocin