title: Method development by GC-ECD and HS-SPME-GC-MS for beer volatile analysis.
abstract: Two methods for the extraction, identification and quantification of the highest occurrence and lowest perception threshold off-flavours in fifteen different samples of Brazilian Pilsner beers were developed. For this purpose, headspace solid phase microextraction in combination with a gas chromatography coupled to a mass spectrometric detection (HS-SPME-GC-MS) as well as headspace extraction in combination with a gas chromatography coupled to electron capture detection (HS-GC-ECD) were evaluated. The first and the second methods were used for esters and vicinal diketones analysis, respectively. All data were comprehended below the taster's threshold detection limit: ethyl acetate 39.48 ng mL(-1) (RSD mean value 4.2%), isoamyl acetate 3.88 ng mL(-1) (RSD mean value 3.4%), ethyl hexanoate 0.61 ng mL(-1) (RSD mean value 3.1%) and 2,3-butanedione 0.10 ng mL(-1) (RSD mean value 2.9%). The validated method demonstrated to be useful for the analysis of highest incidence beer off-flavours.
authors.affiliation: Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro (IFRJ), Mestrado Profissional em Ciência e Tecnologia de Alimentos (PCTA), Rua Senador Furtado, 121, 20270-021 Rio de Janeiro, RJ, Brazil. Electronic address: gisele.dasilva@ig.com.br.
authors.forename: Gisele C
authors.initials: GC
authors.lastname: da Silva
authors.affiliation: Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro (IFRJ), Mestrado Profissional em Ciência e Tecnologia de Alimentos (PCTA), Rua Senador Furtado, 121, 20270-021 Rio de Janeiro, RJ, Brazil.
authors.forename: Abner A S
authors.initials: AA
authors.lastname: da Silva
authors.affiliation: Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro (IFRJ), Mestrado Profissional em Ciência e Tecnologia de Alimentos (PCTA), Rua Senador Furtado, 121, 20270-021 Rio de Janeiro, RJ, Brazil.
authors.forename: Larissa S N
authors.initials: LS
authors.lastname: da Silva
authors.affiliation: Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro (IFRJ), Mestrado Profissional em Ciência e Tecnologia de Alimentos (PCTA), Rua Senador Furtado, 121, 20270-021 Rio de Janeiro, RJ, Brazil.
authors.forename: Ronoel Luiz de O
authors.initials: RL
authors.lastname: Godoy
authors.affiliation: Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro (IFRJ), Mestrado Profissional em Ciência e Tecnologia de Alimentos (PCTA), Rua Senador Furtado, 121, 20270-021 Rio de Janeiro, RJ, Brazil.
authors.forename: Luciana C
authors.initials: LC
authors.lastname: Nogueira
authors.affiliation: Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro (IFRJ), Mestrado Profissional em Ciência e Tecnologia de Alimentos (PCTA), Rua Senador Furtado, 121, 20270-021 Rio de Janeiro, RJ, Brazil.
authors.forename: Simone L
authors.initials: SL
authors.lastname: Quitério
authors.affiliation: Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro (IFRJ), Mestrado Profissional em Ciência e Tecnologia de Alimentos (PCTA), Rua Senador Furtado, 121, 20270-021 Rio de Janeiro, RJ, Brazil.
authors.forename: Renata S L
authors.initials: RS
authors.lastname: Raices
mesh_terms: D001515:Beer / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D052617:Solid Phase Microextraction / Q000379:methods*
keywords: Beer; Gas chromatography; HS (headspace); Off-flavours; SPME (solid-phase microextraction)