title: The effect of fibroblast growth factor 9 on the osteogenic differentiation of calvaria-derived mesenchymal cells.
abstract: Fibroblast growth factor 9 (FGF9) plays complicated and crucial roles in bone formation, and the biologic effect of FGF9 may depend on the gene dosage, developmental stage, cell type, or interactions with other cytokines. In this study, we demonstrated that FGF9 enhanced the phosphorylation of extracellular regulated protein kinases 1/2 in calvaria-derived mesenchymal cells. However, the inhibitory effect of FGF9 on the osteogenic differentiation of calvaria-derived mesenchymal cells did not depend on the phosphorylation of extracellular regulated protein kinases 1/2. Combined with the previous findings that FGF9 promotes dental pulp stem cells chondrogenesis in vitro, we suggest that FGF9 may be applied to promote chondrogenesis and inhibit osteogenesis in mesenchymal stem cells in vitro.
authors.affiliation: From the *Department of Oral & Cranio-maxillofacial Science, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology; and †Oral Bioengineering Laboratory, Shanghai Research Institute of Stomatology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
authors.forename: Jingting
authors.initials: J
authors.lastname: Lu
authors.forename: Jiewen
authors.initials: J
authors.lastname: Dai
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
authors.forename: Maolin
authors.initials: M
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Sun
authors.forename: XiuLi
authors.initials: X
authors.lastname: Zhang
authors.forename: Hongbo
authors.initials: H
authors.lastname: Yu
authors.forename: WenBin
authors.initials: W
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Xinquan
authors.initials: X
authors.lastname: Jiang
authors.forename: Guofang
authors.initials: G
authors.lastname: Shen
mesh_terms: D000469:Alkaline Phosphatase / Q000187:drug effects; D000818:Animals; D002070:Butadienes / Q000494:pharmacology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000187:drug effects; D024042:Collagen Type I / Q000187:drug effects; D050659:Core Binding Factor Alpha 1 Subunit / Q000187:drug effects; D003470:Culture Media; D004791:Enzyme Inhibitors / Q000494:pharmacology; D051525:Fibroblast Growth Factor 9 / Q000494:pharmacology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D019950:Mitogen-Activated Protein Kinase 1 / Q000037:antagonists & inhibitors / Q000187:drug effects; D048052:Mitogen-Activated Protein Kinase 3 / Q000037:antagonists & inhibitors / Q000187:drug effects; D009570:Nitriles / Q000494:pharmacology; D015675:Osteocalcin / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D053495:Osteopontin / Q000187:drug effects; D010766:Phosphorylation; D012886:Skull / Q000166:cytology / Q000187:drug effects
chemical_list: D002070:Butadienes; D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D003470:Culture Media; D004791:Enzyme Inhibitors; C496460:Fgf9 protein, mouse; D051525:Fibroblast Growth Factor 9; D009570:Nitriles; C493716:Runx2 protein, mouse; C506896:Spp1 protein, mouse; C113580:U 0126; D015675:Osteocalcin; D053495:Osteopontin; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D000469:Alkaline Phosphatase