title: Celery oil modulates DEHP-induced reproductive toxicity in male rats.
abstract: The objective of the study was to investigate the protective effect of Apium graveolens (AP) against di-(2-ethylhexyl) phthalate (DEHP)-induced testes injury in rats. Adult rats were divided into nine groups: (1) control group (no treatment); (2) corn oil (60 μg/kg body weight - bwt); (3) AP (50 μg/kg bwt); (4) 300 mg DEHP/kg bwt; (5) 500 mg DEHP/kg bwt; (6) 1000 mg DEHP/kg bwt; (7) 300 mg DEHP/kg bwt+AP; (8) 500 mg DEHP/kg bwt+AP; and (9) 1000 mg DEHP/kg bwt+AP. Oral administration of treatments was performed daily for 6 weeks. DEHP decreased (p<0.01) body weight, testis weight and serum concentrations of testosterone, cholesterol and total proteins. Moreover, DEHP increased (p<0.001) total antioxidant capacity in the testis and plasma DEHP level. In addition, DEHP decreased mRNA expression of two testicular steroidogenic enzymes: 3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase. DEHP also caused atrophy, vacuolar degeneration and aspermia of the seminiferous tubules. AP administered concurrently with DEHP effectively alleviated most of the DEHP-induced effects. In conclusion, in male rats, DEHP had adverse effects on the testis including inhibition of androgen production. A concurrent administration of A. graveolens (celery oil) protected the testis against DEHP-induced toxicity.
authors.affiliation: Department of Zoology, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt. Electronic address: monaamhelal@yahoo.com.
authors.forename: Mona A M
authors.initials: MA
authors.lastname: Helal
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D015096:3-Hydroxysteroid Dehydrogenases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D000728:Androgens / Q000009:adverse effects / Q000627:therapeutic use; D000818:Animals; D000975:Antioxidants / Q000009:adverse effects / Q000627:therapeutic use; D000077102:Apium / Q000737:chemistry*; D004051:Diethylhexyl Phthalate / Q000008:administration & dosage / Q000037:antagonists & inhibitors* / Q000493:pharmacokinetics / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004534:Egypt; D052244:Endocrine Disruptors / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics / Q000633:toxicity; D020834:Ethnopharmacology; D005638:Fruit / Q000737:chemistry; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007248:Infertility, Male / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D016488:Medicine, African Traditional; D009822:Oils, Volatile / Q000009:adverse effects / Q000627:therapeutic use*; D009929:Organ Size / Q000187:drug effects; D008517:Phytotherapy / Q000009:adverse effects; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
chemical_list: D000728:Androgens; D000975:Antioxidants; D052244:Endocrine Disruptors; D009822:Oils, Volatile; D004051:Diethylhexyl Phthalate; D015067:17-Hydroxysteroid Dehydrogenases; D015096:3-Hydroxysteroid Dehydrogenases; C477269:3 beta-hydroxy-delta 5-steroid dehydrogenase, rat
keywords: 17β-HSD; 3β-HSD; Cholesterol; Testes; Testosterone