title: Subcellular distribution and chemical forms of cadmium in the edible seaweed, Porphyra yezoensis.
abstract: The subcellular distribution and chemical forms of Cd were investigated in the edible seaweed, Porphyra yezoensis. The seaweed was exposed to different Cd concentrations (0.01, 0.05, 0.1, 0.5, 1.0 and 5.0mgl(-1)) for up to 96h. In both the controls (no Cd added) and treatment groups, 41.2-79.2% of Cd was localised in the cell wall, and the proportion of Cd in the cell wall increased with increasing concentrations of Cd and exposure time. In the control groups, 74.8% of Cd was extracted by 1M NaCl, followed by 2% acetic acid, HAC (18.9%). In the treatment groups, most Cd was extracted by 2% HAC. The proportion of Cd extracted by 2% HAC increased with exposure to increasing concentrations of Cd and over time. Cell wall deposition and forming of precipitates with phosphate may be a key strategy to reduce Cd toxicity in P. yezoensis.
authors.affiliation: Yellow Sea Fishery Research Institute Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Ocean and Fishery Bureau of Huangdao District of Qingdao City, Qingdao 266400, China.
authors.forename: Jifa
authors.initials: J
authors.lastname: Wu
authors.affiliation: Yellow Sea Fishery Research Institute Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, China. Electronic address: yanfang_z1979@163.com.
authors.forename: Derong
authors.initials: D
authors.lastname: Shang
authors.affiliation: Yellow Sea Fishery Research Institute Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Ning
authors.affiliation: Yellow Sea Fishery Research Institute Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, China.
authors.forename: Yuxiu
authors.initials: Y
authors.lastname: Zhai
authors.affiliation: Yellow Sea Fishery Research Institute Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Sheng
authors.affiliation: Yellow Sea Fishery Research Institute Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Ding
mesh_terms: D002104:Cadmium / Q000032:analysis*; D002473:Cell Wall / Q000737:chemistry; D005506:Food Contamination / Q000032:analysis*; D044664:Porphyra / Q000737:chemistry*; D012624:Seaweed / Q000737:chemistry*
chemical_list: D002104:Cadmium
keywords: Acetic acid (PubChem CID: 176); Cadmium; Cadmium dichloride (PubChem CID: 24633); Cadmium ion (PubChem CID: 31193); Cadmium oxalate (PubChem CID: 3083647); Cadmium phosphates (PubChem CID: 26060); Chemical form; Dithiothreitol (PubChem CID: 19001); Ethanol (PubChem CID: 702); Organo-ligands (PubChem CID: 11403475); Porphyra yezoensis; Sodium chloride (PubChem CID: 5234); Subcellular distribution; Sucrose (PubChem CID: 5988); Tolerance mechanism