title: Dextran sulfate sodium-induced ulcerative colitis leads to testicular toxicity in mice: Role of inflammation, oxidative stress and DNA damage.
abstract: Ulcerative colitis is associated with an alteration in gonadal hormones and affects testicular weight in rodents. However, association of ulcerative colitis with testicular damage is not clearly known. Ulcerative colitis was induced using 5% (w/v) dextran sulfate sodium in normal drinking water for 1, 7-day cycle in short-term study and 2.5% (w/v) dextran sulfate sodium in normal drinking water for 4 cycles with 2 weeks remission period between each cycle in long-term study. Ulcerative colitis was associated with a significant increase in inflammation, oxidative stress, DNA damage in testes and sperm DNA damage and a significant decrease in the epididymal sperm count and 3β-HSD expression. No difference was observed in the plasma testosterone levels between control and treatment groups. In the present study, ulcerative colitis was associated with testicular damage, and juvenile mice were found to be more sensitive than adult mice.
authors.affiliation: Facility for Risk Assessment and Intervention Studies, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, Punjab 160 062, India. Electronic address: amisha.parmar2012@gmail.com.
authors.forename: A R
authors.initials: AR
authors.lastname: Parmar
authors.affiliation: Facility for Risk Assessment and Intervention Studies, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, Punjab 160 062, India. Electronic address: priyankatrvd2@gmail.com.
authors.forename: P P
authors.initials: PP
authors.lastname: Trivedi
authors.affiliation: Facility for Risk Assessment and Intervention Studies, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, Punjab 160 062, India. Electronic address: gbjena@gmail.com.
authors.forename: G B
authors.initials: GB
authors.lastname: Jena
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000818:Animals; D003093:Colitis, Ulcerative / Q000139:chemically induced* / Q000150:complications; D004249:DNA Damage / Q000187:drug effects*; D016264:Dextran Sulfate / Q000009:adverse effects / Q000633:toxicity*; D007249:Inflammation / Q000139:chemically induced*; D015850:Interleukin-6 / Q000032:analysis; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D013076:Sperm Count; D013094:Spermatozoa / Q000187:drug effects; D013737:Testis / Q000737:chemistry / Q000187:drug effects*; D013739:Testosterone / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D013739:Testosterone; D016264:Dextran Sulfate; D015096:3-Hydroxysteroid Dehydrogenases
keywords: DNA damage; Inflammation; Mice; Oxidative stress; Testes; Ulcerative colitis