title: Regulation of the biological functions of osteoblasts and bone formation by Zn-incorporated coating on microrough titanium.
abstract: To improve the biological performance of titanium implant, a series of Zn-incorporated coatings were fabricated on the microrough titanium (Micro-Ti) via sol-gel method by spin-coating technique. The successful fabrication of the coating was verified by combined techniques of scanning electron microscopy, surface profiler, X-ray diffraction, X-ray photoelectron spectroscopy, and water contact angle measurements. The incorporated zinc existed as ZnO, which released Zn ions in a sustained manner. The Zn-incorporated samples (Ti-Zn0.08, Ti-Zn0.16, and Ti-Zn0.24) efficiently inhibited the adhesion of both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria. The in vitro evaluations including cell activity, alkaline phosphatase (ALP), mineralization, osteogenic genes expressions (Runx2, ALP, OPG, Col I, OPN, and OC), and tartrate-resistant acid phosphatase, confirmed that Ti-Zn0.16 sample was the optimal one to regulate the proliferation or differentiation for both osteoblasts and osteoclasts. More importantly, in vivo evaluations including Micro-CT analysis, push-out test, and histological observations verified that Ti-Zn0.16 implants could efficiently promote new bone formation after implantation for 4 and 12 weeks, respectively. The resulting material thus has potential application in orthopedic field.
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education College of Bioengineering, Chongqing University , Chongqing 400044, P. R. China.
authors.forename: Xinkun
authors.initials: X
authors.lastname: Shen
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Gaoqiang
authors.initials: G
authors.lastname: Xu
authors.forename: Weizhen
authors.initials: W
authors.lastname: Chen
authors.forename: Kui
authors.initials: K
authors.lastname: Xu
authors.forename: Qichun
authors.initials: Q
authors.lastname: Ran
authors.forename: Pingping
authors.initials: P
authors.lastname: Ma
authors.forename: Yarong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jinghua
authors.initials: J
authors.lastname: Li
authors.forename: Kaiyong
authors.initials: K
authors.lastname: Cai
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D005269:Femur / Q000601:surgery; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000637:transplantation; D010012:Osteogenesis / Q000187:drug effects; D011817:Rabbits; D051381:Rats; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D015032:Zinc / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D014025:Titanium; D015032:Zinc
keywords: in vivo; osteoblasts; osteogenesis; titanium; zinc