title: Effect of DC-CIK cell on the proliferation, apoptosis and differentiation of leukemia cells.
abstract: OBJECTIVE
  To observe the effect of co-culture cytokine-induced killer cells (CIK) and homologous dendritic cells (DC) on the proliferative activity and phenotype change of the DC-CIK cell and the cell killing activity of leukemia HL-60.
  
  
  METHODS
  50 mL cord blood sample was obtained from infants delivered by full term healthy woman and the cord blood mononuclear cells were isolated by density gradient centrifugation. Non-adherent cells were collectedfor the induction culture of CIK, adherent cells were differentiated into mature DC; cultured mature DC was mixed with and CIK in the proportion of 1:5 for 12 d. Killing activity of DC-CIK co-cultured cell on leukemia HL-60 was detected by MTT assay.
  
  
  RESULTS
  Compared with CIKs, the co-cultured DC-CIKs presented a markedly higher proliferation and killing activity.
  
  
  CONCLUSIONS
  Co-culture of DC-CIK cells led to a significant increase of the proliferation and cytotoxicity of CIK.
authors.affiliation: Department of Blood Transfusion, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China.
authors.forename: Hui-Qing
authors.initials: HQ
authors.lastname: Qu
authors.affiliation: Department of Neurology, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China.
authors.forename: Xiao-Sheng
authors.initials: XS
authors.lastname: Zhou
authors.affiliation: Pharmaceutical Department, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Zhou
authors.affiliation: Laboratory Department, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D018920:Coculture Techniques; D055612:Cytokine-Induced Killer Cells / Q000166:cytology*; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology*; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia; D010641:Phenotype
chemical_list: D016207:Cytokines
keywords: Adoptive; Adoptive immunotherapy; Dendritic cells; Fetal blood; HL-60; Immunotherapy