title: Antidepressant effect and categorization of inhibitory activity of monoamine oxidase type A and B of ethanolic extract of seeds of Trigonella foenum graecum Linn.
abstract: Trigonella foenum- graecum Linn (Fabaceae) is an annual aromatic herb and no wit is cultivated globally like in Pakistan, Egypt, India, Middle East etc. Traditionally it was used in anorexia, as febrifuge, to soothe gastritis and gastric ulcers, as a galactagogue and as condiment, hypoglycemic agent and employed in various as nervous disorders. The study aimed to investigate the antidepressant effect of ethanolic extract of seeds of Trigonella foenum graecum and underlying mechanism of action. For assessment of antidepressant activity Forced Swimming Test (FST), Tail Suspension Test (TST), Monoamine (MAO) Assay and Locomotor Activity Test were studied. Acute toxicity, Rota Rod and Grip Strength Tests were also performed. The significant declining in immobility time as compared to control was shown in Forced swimming test as compared to tail suspension test. Considerable change was not found in open field test (OFT). EtOH extract of seeds of fenugreek represent maximum significant reduction which was 30 and 24.65% in MAO- A and B activity respectively in the rat's whole brain as compared to control animals in Monoamine oxidase (MAO) assay. All tested doses were found ineffective in impairment of muscle coordination in Rota rod and in grip strength related to muscle relaxant property. According to experimental findings it is revealed that ethanolic extract of seeds of Trigonella foenum graecum showed antidepressant effect by inhibiting the activity of MAO-A and B.
authors.affiliation: Research Institute of Pharmaceutical sciences, Department of Pharmacognosy, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan.
authors.forename: Raheela
authors.initials: R
authors.lastname: Khursheed
authors.affiliation: Research Institute of Pharmaceutical sciences, Department of Pharmacognosy, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan.
authors.forename: Ghazala H
authors.initials: GH
authors.lastname: Rizwani
authors.affiliation: Department of Biochemistry, University of Karachi, Karachi, Pakistan.
authors.forename: Viqar
authors.initials: V
authors.lastname: Sultana
authors.affiliation: Research Institute of Pharmaceutical sciences, Department of Pharmacognosy, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan.
authors.forename: Maryam
authors.initials: M
authors.lastname: Ahmed
authors.affiliation: Department of Chemistry, Faculty of Pharmacy, Federal Urdu University, Karachi, Pakistan.
authors.forename: Arfa
authors.initials: A
authors.lastname: Kamil
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects*; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000737:chemistry*; D008297:Male; D008996:Monoamine Oxidase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D009043:Motor Activity / Q000187:drug effects; D053580:Muscle Strength / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar; D045442:Rotarod Performance Test; D012639:Seeds; D012997:Solvents / Q000737:chemistry*; D013550:Swimming; D029910:Trigonella / Q000737:chemistry
chemical_list: D000928:Antidepressive Agents; D008996:Monoamine Oxidase Inhibitors; D010936:Plant Extracts; D012997:Solvents; D000431:Ethanol