title: Adhesion and proliferation of human periodontal ligament cells on poly(2-methoxyethyl acrylate).
abstract: Human periodontal ligament (PDL) cells obtained from extracted teeth are a potential cell source for tissue engineering. We previously reported that poly(2-methoxyethyl acrylate) (PMEA) is highly biocompatible with human blood cells. In this study, we investigated the adhesion, morphology, and proliferation of PDL cells on PMEA and other types of polymers to design an appropriate scaffold for tissue engineering. PDL cells adhered and proliferated on all investigated polymer surfaces except for poly(2-hydroxyethyl methacrylate) and poly[(2-methacryloyloxyethyl phosphorylcholine)-co-(n-butyl methacrylate)]. The initial adhesion of the PDL cells on PMEA was comparable with that on polyethylene terephthalate (PET). In addition, the PDL cells on PMEA spread well and exhibited proliferation behavior similar to that observed on PET. In contrast, platelets hardly adhered to PMEA. PMEA is therefore expected to be an excellent scaffold for tissue engineering and for culturing tissue-derived cells in a blood-rich environment.
authors.affiliation: Graduate School of Science and Engineering, Yamagata University, Jonan 4-3-16, Yonezawa, Yamagata 992-8510, Japan.
authors.forename: Erika
authors.identifier: 0000-0002-3667-9567
authors.initials: E
authors.lastname: Kitakami
authors.affiliation: Graduate School of Science and Engineering, Yamagata University, Jonan 4-3-16, Yonezawa, Yamagata 992-8510, Japan.
authors.forename: Makiko
authors.initials: M
authors.lastname: Aoki
authors.affiliation: Graduate School of Science and Engineering, Yamagata University, Jonan 4-3-16, Yonezawa, Yamagata 992-8510, Japan.
authors.forename: Chikako
authors.initials: C
authors.lastname: Sato
authors.affiliation: Graduate School of Dentistry, Division of Periodontology and Endodontology, Tohoku University, Seiryo-machi 4-1, Sendai, Miyagi 980-8575, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishihata
authors.affiliation: Graduate School of Science and Engineering, Yamagata University, Jonan 4-3-16, Yonezawa, Yamagata 992-8510, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Tanaka
mesh_terms: D000179:Acrylates / Q000737:chemistry / Q000627:therapeutic use*; D001672:Biocompatible Materials / Q000627:therapeutic use*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D010513:Periodontal Ligament / Q000187:drug effects; D011092:Polyethylene Glycols / Q000737:chemistry / Q000627:therapeutic use; D011093:Polyethylene Terephthalates; D011108:Polymers / Q000737:chemistry / Q000627:therapeutic use*; D013499:Surface Properties; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D000179:Acrylates; D001672:Biocompatible Materials; D011093:Polyethylene Terephthalates; D011108:Polymers; C413485:poly(2-methoxyethylacrylate); D011092:Polyethylene Glycols