title: Sensitive electrochemical detection of NADH and ethanol at low potential based on pyrocatechol violet electrodeposited on single walled carbon nanotubes-modified pencil graphite electrode.
abstract: In this work, the electrodeposition of pyrocatechol violet (PCV) was initially investigated by the electrochemical surface plasmon resonance (ESPR) technique. Subsequently, PCV was used as redox-mediator and was electrodeposited on the surface of pencil graphite electrode (PGE) modified with single-wall carbon nanotubes (SWCNTs). Owing to the remarkable synergistic effect of SWCNTs and PCV, PGE/SWCNTs/PCV exhibited excellent electrocatalytic activity towards dihydronicotinamide adenine dinucleotide (NADH) oxidation at low potential (0.2V vs. SCE) with fast amperometric response (<10s), broad linear range (1.3-280 μM), good sensitivity (146.2 μA mM(-1)cm(-2)) and low detection limit (1.3 μM) at signal-to-noise ratio of 3. Thus, this PGE/SWCNTs/PCV could be further used to fabricate a sensitive and economic ethanol biosensor using alcohol dehydrogenase (ADH) via a glutaraldehyde/BSA cross-linking procedure.
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Wu
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address: danshan@njust.edu.cn.
authors.forename: Dan
authors.initials: D
authors.lastname: Shan
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Pei-Xin
authors.initials: PX
authors.lastname: Yuan
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address: xueji@usf.edu.
authors.forename: Xue-Ji
authors.initials: XJ
authors.lastname: Zhang
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry; D001557:Benzenesulfonates / Q000737:chemistry*; D015374:Biosensing Techniques; D004563:Electrochemistry; D004566:Electrodes; D004595:Electroplating / Q000379:methods*; D000431:Ethanol / Q000032:analysis*; D006108:Graphite / Q000737:chemistry*; D009243:NAD / Q000032:analysis*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: D001557:Benzenesulfonates; D037742:Nanotubes, Carbon; D009243:NAD; C009134:pyrocatechol violet; D000431:Ethanol; D006108:Graphite; D000426:Alcohol Dehydrogenase
keywords: Dihydronicotinamide adenine dinucleotide; Ethanol; Pencil graphite electrode; Pyrocatechol violet; Single-walled carbon nanotubes