title: Separation of miscarriage tissue from maternal decidua for chromosome analysis.
abstract: OBJECTIVE
  To demonstrate a technique for separation of miscarriage tissue from maternal decidua to reduce maternal cell contamination for chromosome analysis.
  
  
  METHODS
  Retrospective.
  
  
  METHODS
  University-based infertility center.
  
  
  METHODS
  Not applicable.
  
  
  METHODS
  Retrospective collection of de-identified images and video from manual vacuum aspiration (MVA) performed after first-trimester pregnancy losses. This project was exempt from institutional review board approval as no patient-identifying data were used.
  
  
  METHODS
  Reduction of maternal cell contamination and improvement of the accuracy of chromosome analysis.
  
  
  RESULTS
  Video demonstration of separation of miscarriage tissue from maternal decidua after MVA.
  
  
  CONCLUSIONS
  Chromosome analysis of a miscarriage is performed to assess the etiology of miscarriage and recurrent pregnancy loss. However, maternal cell contamination can limit the accuracy. This video demonstrates a technique for separating miscarriage tissue from maternal decidua after MVA to reduce maternal cell contamination prior to sending tissue for analysis. The same technique has been used in our clinic with first-trimester dilation and curettage using mechanical suction.
authors.affiliation: Department of Reproductive Endocrinology and Infertility, Stanford Hospital & Clinics, Stanford, California. Electronic address: gm807@stanford.edu.
authors.forename: Gayathree
authors.initials: G
authors.lastname: Murugappan
authors.affiliation: Department of Reproductive Endocrinology and Infertility, Stanford Hospital & Clinics, Stanford, California.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Gustin
authors.affiliation: Department of Reproductive Endocrinology and Infertility, Stanford Hospital & Clinics, Stanford, California.
authors.forename: Ruth B
authors.initials: RB
authors.lastname: Lathi
mesh_terms: D000022:Abortion, Spontaneous / Q000235:genetics* / Q000473:pathology; D002877:Chromosomes, Human; D003656:Decidua / Q000473:pathology; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007621:Karyotyping; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D012189:Retrospective Studies; D012307:Risk Factors; D013048:Specimen Handling / Q000379:methods*; D014619:Vacuum Curettage
keywords: Recurrent pregnancy loss; chromosome analysis; karyotype analysis; maternal cell contamination; miscarriage