title: Proteomics and metabolomics for in situ monitoring of wound healing.
abstract: Wound healing of soft tissue and bone defects is a complex process in which cellular differentiation and adaption are regulated by internal and external factors, among them are many different proteins. In contrast to insights into the significance of various single proteins based on model systems, the knowledge about the processes at the actual site of wound healing is still limited. This is caused by a general lack of methods that allow sampling of extracellular factors, metabolites, and proteins in situ. Sampling of wound fluids in combination with proteomics and metabolomics is one of the promising approaches to gain comprehensive and time resolved data on effector molecules. Here, we describe an approach to sample metabolites by microdialysis and to extract proteins simultaneously by adsorption. With this approach it is possible (i) to collect, enrich, and purify proteins for a comprehensive proteome analysis; (ii) to detect more than 600 proteins in different defects including more than 100 secreted proteins, of which many proteins have previously been demonstrated to have diagnostic or predictive power for the wound healing state; and (iii) to combine continuous sampling of cytokines and metabolites and discontinuous sampling of larger proteins to gain complementary information of the same defect.
authors.affiliation: Department of Proteomics, Helmholtz-Centre for Environmental Research-UFZ, Permoserstraße 15, 04318 Leipzig, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Kalkhof
authors.affiliation: University Center of Orthopedics and Trauma Surgery, University Hospital "Carl Gustav Carus", TU Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Förster
authors.affiliation: Department of Proteomics, Helmholtz-Centre for Environmental Research-UFZ, Permoserstraße 15, 04318 Leipzig, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Schmidt
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Hospital "Carl Gustav Carus", TU Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Matthias C
authors.initials: MC
authors.lastname: Schulz
authors.affiliation: Department of Metabolomics, Helmholtz-Centre for Environmental Research-UFZ, Permoserstraße 15, 04318 Leipzig, Germany ; Institute of Pharmacy, Faculty of Biosciences, Pharmacy and Psychology, University of Leipzig, 04103 Leipzig, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Baumann
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Hospital "Carl Gustav Carus", TU Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Weißflog
authors.affiliation: University Center of Orthopedics and Trauma Surgery, University Hospital "Carl Gustav Carus", TU Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Wenling
authors.initials: W
authors.lastname: Gao
authors.affiliation: Institute of Physiological Chemistry, TU Dresden, Fiedlerstraße 42, 01307 Dresden, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Hempel
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Hospital "Carl Gustav Carus", TU Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Eckelt
authors.affiliation: University Center of Orthopedics and Trauma Surgery, University Hospital "Carl Gustav Carus", TU Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Rammelt
authors.affiliation: Department of Proteomics, Helmholtz-Centre for Environmental Research-UFZ, Permoserstraße 15, 04318 Leipzig, Germany ; Department of Metabolomics, Helmholtz-Centre for Environmental Research-UFZ, Permoserstraße 15, 04318 Leipzig, Germany ; Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, 9000 Aalborg, Denmark.
authors.forename: Martin
authors.initials: M
authors.lastname: von Bergen
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000235:genetics; D057785:Catheters; D005786:Gene Expression Regulation; D006801:Humans; D055432:Metabolomics; D017551:Microdialysis; D014176:Protein Biosynthesis / Q000235:genetics; D040901:Proteomics; D051381:Rats; D014945:Wound Healing / Q000235:genetics*