title: Copy number differences of Y chromosomal genes between superior and inferior quality semen producing crossbred (Bos taurus × Bos indicus) bulls.
abstract: The removal of crossbred bulls from semen collection programs due to the production of poor quality semen causes substantial monetary losses to the dairy industry. Seminal quality, a quantitative trait, is greatly influenced by genome level variations. Deletion and/or duplication of Y chromosomal genes and subsequent changes in gene copy number have a major role in determining spermatogenic efficiency and, therefore, seminal quality. In this study, copy numbers of three Y chromosomal genes TSPY, DDX3Y, and USP9Y in genomic DNA were estimated and compared in two groups of crossbred (Bos taurus × Bos indicus) bulls of ten each, superior and inferior quality semen producing bulls, which were classified based on their seminal quality parameters. For TSPY gene, the inferior quality semen donor group has significantly lower copy number than superior quality semen donor group (p < 0.05). No significant difference was found in DDX3Y and USP9Y gene copy numbers between two groups (p > 0.05). In conclusion, this study demonstrates that the copy number of TSPY, a Y chromosomal spermatogenesis related gene, may be an important determinant to predict the quality of bull semen, facilitating better selection of bulls in a herd for semen collection program.
authors.affiliation: a Animal Genomics Lab, Animal Biotechnology Center , National Dairy Research Institute , Karnal , India.
authors.forename: Ayan
authors.initials: A
authors.lastname: Mukherjee
authors.forename: Gulshan
authors.initials: G
authors.lastname: Dass
authors.forename: Jagan
authors.initials: J
authors.lastname: Mohanarao G
authors.forename: Vinay Kumar
authors.initials: VK
authors.lastname: Katneni
authors.forename: Dipak
authors.initials: D
authors.lastname: Banerjee
authors.forename: Tapan Kumar
authors.initials: TK
authors.lastname: Das
authors.forename: Moloya
authors.initials: M
authors.lastname: Gohain
authors.forename: A K
authors.initials: AK
authors.lastname: Chakrabarty
authors.forename: Tirtha Kumar
authors.initials: TK
authors.lastname: Datta
authors.forename: Sachinandan
authors.initials: S
authors.lastname: De
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics; D053487:DEAD-box RNA Helicases / Q000235:genetics; D018628:Gene Dosage; D006824:Hybridization, Genetic; D008297:Male; D012661:Semen / Q000502:physiology*; D043222:Ubiquitin Thiolesterase / Q000235:genetics; D014998:Y Chromosome
chemical_list: D018797:Cell Cycle Proteins; D043222:Ubiquitin Thiolesterase; D053487:DEAD-box RNA Helicases
keywords: Absolute copy number; Crossbred bull; DDX3Y gene; TSPY gene; USP9Y gene; Y chromosome