title: Next generation sequence analysis of the transcriptome of Sydney rock oysters (Saccostrea glomerata) exposed to a range of environmental stressors.
abstract: Sydney rock oysters (Saccostrea glomerata) were exposed to environmental stressors at contaminated field sites or in a controlled laboratory setting. RNA seq transcriptome data were generated for the gill and digestive gland using Roche's 454 pyrosequencing technology. 28,685 contigs were de novo assembled which encoded 11,671 different protein products. The data will act as a reference for future studies in ecology, immunology and environmental toxicology.
authors.affiliation: CSIRO Centre for Environmental Contaminants Research, Land and Water, Locked Bag 2007, Kirrawee, NSW 2232, Australia. Electronic address: Sharon.Hook@csiro.au.
authors.forename: Sharon E
authors.initials: SE
authors.lastname: Hook
authors.affiliation: Evolution and Ecology Research Centre School of Biological, Earth and Environmental Sciences, UNSW Australia, Sydney, NSW 2052, Australia.
authors.forename: Emma L
authors.initials: EL
authors.lastname: Johnston
authors.affiliation: Department of Biological Sciences, Macquarie University, Sydney, 2109 NSW, Australia.
authors.forename: Sham
authors.initials: S
authors.lastname: Nair
authors.affiliation: NSW Dept of Environment, Climate Change & Water (DECCW), Australia.
authors.forename: Anthony C
authors.initials: AC
authors.lastname: Roach
authors.affiliation: CSIRO Digital Productivity and Services, Acton, ACT 2601, Australia.
authors.forename: Philippe
authors.initials: P
authors.lastname: Moncuquet
authors.affiliation: NSW Systems Biology Initiative, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: Natalie A
authors.initials: NA
authors.lastname: Twine
authors.affiliation: Department of Biological Sciences, Macquarie University, Sydney, 2109 NSW, Australia.
authors.forename: David A
authors.initials: DA
authors.lastname: Raftos
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004777:Environment; D020869:Gene Expression Profiling; D005880:Gills / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D010125:Ostreidae / Q000235:genetics* / Q000502:physiology; D017423:Sequence Analysis, RNA; D013312:Stress, Physiological / Q000235:genetics* / Q000502:physiology; D059467:Transcriptome
chemical_list: D017931:DNA Primers
keywords: 454 pyrosequencing; Immune response; Metals; Oil; Oyster; RNA seq