title: A feedback expression of microRNA-590 and activating transcription factor-3 in human breast cancer cells.
abstract: MicroRNAs (miRNAs) are small non coding RNA molecules (∼ 23 nt) that are capable of regulating several physiological and pathological processes by targeting mRNAs post transcriptionally, and miRNAs are also known to be regulated by their own target gene(s) in a feedback manner. In this study, we analysed the expression of miRNAs (pre-mir-93, pre-mir-20b, pre-mir-520 c, pre-mir-143, pre-mir-154 and pre-mir-590) by body map, an in silico method and by qRT-PCR in MDA-MB231 (highly invasive and metastatic in nature), and MCF-7 (poor invasive and metastatic in nature) cells. These miRNAs were down regulated in MDA-MB231 cells, and among these, miR-590 was found to putatively target activating transcription factor-3 (ATF-3), a stress response gene. ATF-3 expression level was significantly increased in MDA-MB231 cells and inhibition of ATF-3 expression in these cells increased the expression of pre-mir-590. Thus, these results suggest that there is a negative feedback expression of pre-mir-590 and its putative target gene, ATF-3 in human breast cancer cells.
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India.
authors.forename: P J
authors.initials: PJ
authors.lastname: Miranda
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Vimalraj
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India. Electronic address: selvamn2@yahoo.com.
authors.forename: N
authors.initials: N
authors.lastname: Selvamurugan
mesh_terms: D051700:Activating Transcription Factor 3 / Q000096:biosynthesis* / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation / Q000235:genetics; D025461:Feedback, Physiological; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D051700:Activating Transcription Factor 3; C568221:MIRN590 microRNA, human; D035683:MicroRNAs
keywords: ATF-3; Breast cancer; MicroRNA-590