title: Comparison of the osteogenic potential of mesenchymal stem cells from the bone marrow and adipose tissue of young dogs.
abstract: BACKGROUND
  The aim of the present study was to compare the osteogenic potential of mesenchymal stem cells extracted from the bone marrow (BM-MSCs) and adipose tissue (AD-MSCs) of young dogs. The following parameters were assessed: dimethyl thiazolyl diphenyl tetrazolium (MTT) conversion, alkaline phosphatase (ALP) activity, collagen and mineralised matrix synthesis, and the expressions of osterix, bone sialoprotein (BSP), and osteocalcin (OC).
  
  
  RESULTS
  MTT conversion was greater in BM-MSCs compared to AD-MSCs after 14 and 21 days of differentiation; ALP activity was greater in differentiated AD-MSCs on day 7; collagen synthesis was greater in BM-MSCs on days 14 and 21; the percentage of mineralized area per field was greater in BM-MSCs compared to AD-MSCs; osterix expression was greater in BM-MSCs in days 14 and 21, and BSP and OC expression levels were greater in BM-MSCs at all the investigation time-points.
  
  
  CONCLUSIONS
  It was concluded that the osteogenic potential was greater in BM-MSCs than AD-MSCs when extracted from young dogs.
authors.forename: Endrigo G  L
authors.initials: EG
authors.lastname: Alves
authors.forename: Rogéria
authors.initials: R
authors.lastname: Serakides
authors.forename: Jankerle N
authors.initials: JN
authors.lastname: Boeloni
authors.forename: Isabel R
authors.initials: IR
authors.lastname: Rosado
authors.forename: Natália M
authors.initials: NM
authors.lastname: Ocarino
authors.forename: Humberto P
authors.initials: HP
authors.lastname: Oliveira
authors.forename: Alfredo M
authors.initials: AM
authors.lastname: Góes
authors.affiliation: Núcleo de Células Tronco e Terapia Celular Animal, NCT-TCA Departamento de Clínica e Cirurgia, Escola de veterinária da Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. cleuzaufmg@gmail.com.
authors.forename: Cleuza M F
authors.initials: CM
authors.lastname: Rezende
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000502:physiology; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology; D002454:Cell Differentiation; D002478:Cells, Cultured; D004285:Dogs; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D013997:Time Factors