title: Remodeling intestinal flora with sleeve gastrectomy in diabetic rats.
abstract: OBJECTIVE
  As a complicated symbiotic system, intestinal flora is reported closely related to the development of type 2 diabetes recently. Sleeve gastrectomy is one of the approaches of bariatric surgery and could improve blood glucose control in type 2 diabetes patients. This study was to explore the relationship between remodeled intestinal flora and glucose metabolism in diabetic rats.
  
  
  METHODS
  20 male diabetic rats were operated; 10 of them underwent sleeve gastrectomy, and 10 of them underwent sham operation. Meanwhile 10 male normal rats underwent sleeve gastrectomy as control. The animals' weight and FBG had been measured. The composition changes of intestinal flora were detected by 16S rDNA sequence analysis.
  
  
  RESULTS
  In diabetic rats, weight and fasting blood glucose decreased significantly after sleeve gastrectomy. However, there was no significant change for weight and blood glucose in normal rats after operation. The intestinal flora of diabetic rats reduced in the proportion of Firmicutes and increased in the proportion of Bacteroidetes after sleeve gastrectomy.
  
  
  CONCLUSIONS
  The change of dominant microorganisms in intestinal flora might play an important role in the glucose metabolism.
authors.affiliation: Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, No. 639, Zhizaoju Road, Shanghai 200011, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Huang
authors.affiliation: Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, No. 639, Zhizaoju Road, Shanghai 200011, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Weng
authors.affiliation: Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, No. 639, Zhizaoju Road, Shanghai 200011, China.
authors.forename: Huixin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, No. 639, Zhizaoju Road, Shanghai 200011, China.
authors.forename: Yingli
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000382:microbiology / Q000601:surgery*; D004195:Disease Models, Animal; D005743:Gastrectomy; D007422:Intestines / Q000382:microbiology*; D008297:Male; D051381:Rats; D021521:Ribotyping; D013997:Time Factors; D015431:Weight Loss
chemical_list: D015415:Biomarkers; D001786:Blood Glucose