title: RCCRT1 is correlated with prognosis and promotes cell migration and invasion in renal cell carcinoma.
abstract: OBJECTIVE
  To investigate the expression pattern of a novel long noncoding ribonucleic acid (RNA), RCCRT1, in renal cell carcinoma (RCC) tissues among the patients with various clinicopathologic features and to detect the role of RCCRT1 in migration and invasion of RCC in vitro.
  
  
  METHODS
  We found out a novel long noncoding RNA, RCCRT1, that expressed differently between high-grade (Fuhrman grade III-IV) and low-grade RCC tissues (Fuhrman grade I-II) by gene chip analysis, then verified it with quantitative polymerase chain reaction. The expression of RCCRT1 was diminished by transfecting with small interfering RNA. RCCRT1 effects were assessed by cell proliferation, cell apoptosis, transwell assay, and wound-healing assay.
  
  
  RESULTS
  Compared with adjacent noncancerous tissues, RCCRT1 is upregulated remarkably in RCC, particularly in high-grade RCC tissues. After analyzing the relative expression of RCCRT1 in various tissues by quantitative reverse transcription polymerase chain reaction and clinicopathologic characteristics of patients, we drew the conclusion that RCCRT1 is associated with clinicopathologic findings such as tumor size, pathologic T stage, tumor grade, lymph node metastasis, and distant metastasis. Furthermore, small interfering RNA-induced depletion of RCCRT1 expression suppressed migration and invasion in RCC cell lines.
  
  
  CONCLUSIONS
  The results of the present study suggest that RCCRT1 promoted migration and invasion of RCC and that RCCRT1 may offer a biomarker to verdict prognosis as well as an attractive new target for prognostic and therapeutic intervention for RCC patients in the future.
authors.affiliation: Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Shangqing
authors.initials: S
authors.lastname: Song
authors.affiliation: Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Zhenjie
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Xuerong
authors.initials: X
authors.lastname: Ye
authors.affiliation: Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Junming
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Huamao
authors.initials: H
authors.lastname: Ye
authors.affiliation: Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai, P.R. China. Electronic address: wlhui@medmail.com.cn.
authors.forename: Linhui
authors.initials: L
authors.lastname: Wang
mesh_terms: D000368:Aged; D017209:Apoptosis; D002292:Carcinoma, Renal Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D034741:RNA, Small Interfering / Q000378:metabolism; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism*; D014945:Wound Healing
chemical_list: C000592633:RCCRT1 noncoding RNA, human; D034741:RNA, Small Interfering; D022661:RNA, Untranslated