title: Integration of the human dermal mast cell into the organotypic co-culture skin model.
abstract: The organotypic co-culture skin model has been providing an advanced approach to the in vitro investigation of the skin. Mast cells, containing various mediators such as tryptase and chymase, are thought to contribute to many physiological and pathological events of the skin interactively with other cells. Here, we introduce an organotypic co-culture skin model which successfully integrates human dermal mast cells for further study of mast cell interactions with fibroblasts and keratinocytes.
authors.affiliation: Klinik für Dermatologie, Venerologie, und Allergologie, Allergie-Centrum-Charité, Charité, Charité-Campus Mitte, Luisenstraße 2, 10117, Berlin, Germany.
authors.forename: Jonghui
authors.initials: J
authors.lastname: Kim
authors.forename: Sven
authors.initials: S
authors.lastname: Guhl
authors.forename: Magda
authors.initials: M
authors.lastname: Babina
authors.forename: Torsten
authors.initials: T
authors.lastname: Zuberbier
authors.forename: Metin
authors.initials: M
authors.lastname: Artuc
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D020405:Dermis / Q000166:cytology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D008407:Mast Cells / Q000166:cytology*; D009924:Organ Culture Techniques / Q000379:methods; D012867:Skin / Q000166:cytology*; D017768:Skin, Artificial