title: Simultaneous determination and pharmacokinetic study of gambogic acid and gambogenic acid in rat plasma after oral administration of Garcinia hanburyi extracts by LC-MS/MS.
abstract: Gambogic acid and gambogenic acid are two major bioactive components of Garcinia hanburyi, and play a pivotal role in biologic activity. In this study, a specific and sensitive liquid chromatography-tandem mass spectrometry was developed and validated for simultaneous determination of gambogic acid and gambogenic acid in rat plasma. Chromatographic separation was achieved on a C18 column using an isocratic elution with methanol-10 m m ammonium acetate buffer-acetic acid (90:10:0.1, v/v/v) as the mobile phase. The detection was performed on a triple-quadrupole tandem mass spectrometer equipped with electrospray positive ionization using multiple reaction monitoring modes. The transitions monitored were m/z 629.3 [M + H](+) → 573.2 for gambogic acid, m/z 631.2 [M + H](+) → 507.2 for gambogenic acid and m/z 444.2 [M + NH4 ](+) → 83.1 for IS. Linear calibration curves were obtained in the concentration range of 2.00-1000 ng/mL for gambogic acid and 0.500-250 ng/mL for gambogenic acid. The lower limits of quantification of gambogic acid and gambogenic acid in rat plasma were 2.00 and 0.500 ng/mL, respectively. The intra- and inter-day precision (RSD) values were <11.7% and accuracy (RE) was -10.6-12.4% at three QC levels for both analytes. The assay was successfully applied to evaluate pharmacokinetics behavior in rats after oral administration of Garcinia hanburyi extracts.
authors.affiliation: Hepatobiliary and Pancreatic Surgery, Liaoning Cancer Hospital and Institute, Shenyang, 110042, China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Hua
authors.forename: Chuang
authors.initials: C
authors.lastname: Liang
authors.forename: Lei
authors.initials: L
authors.lastname: Dong
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Qu
authors.forename: Tong
authors.initials: T
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D029760:Garcinia / Q000737:chemistry*; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D010949:Plasma / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014966:Xanthenes / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D044004:Xanthones / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*
chemical_list: D010936:Plant Extracts; D014966:Xanthenes; D044004:Xanthones; C052659:gambogic acid; C045863:neo-gambogic acid
keywords: Garcinia hanburyi; gambogenic acid; gambogic acid; pharmacokinetic study; simultaneous determination