title: Topological organization of Drosophila Hox genes using DNA fluorescent in situ hybridization.
abstract: DNA fluorescent in situ hybridization (FISH) is the method of choice to study genomic organization at the single-cell level. It has been recently used to study the topological organization of the homeotic bithorax complex (BX-C) in Drosophila as well as to describe long-range genomic interactions between the BX-C and the Antennapedia complex (ANT-C), in addition to other genomic loci. Coupled with immunofluorescence, FISH can be used to study the relative positioning of homeotic genes with nuclear subcompartments, such as Polycomb-group (PcG) bodies, transcription factories, or the nuclear lamina. Here, we describe two multicolor 3D-FISH protocols; one for whole mount Drosophila embryos or larval discs and one for Drosophila-cultured cells. Both methods can be applied to any single copy locus of interest and are compatible with immunostaining (FISH-I).
authors.affiliation: Institut de Génétique Humaine, CNRS UPR-1142, 141 rue de la Cardonille, 34396, Montpellier Cedex 5, France, frederic.bantignies@igh.cnrs.fr.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Bantignies
authors.forename: Giacomo
authors.initials: G
authors.lastname: Cavalli
mesh_terms: D000818:Animals; D004330:Drosophila / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*
chemical_list: D018398:Homeodomain Proteins