title: Negative-charge-functionalized mesoporous silica nanoparticles as drug vehicles targeting hepatocellular carcinoma.
abstract: In this paper, a series of doxorubicin-loaded and negative-charge-functionalized mesoporous silica nanoparticles (DOX-MSN/COOH) was successfully prepared and used for imaging and targeting therapy of hepatocellular carcinoma. The nanoparticles were uniform and negatively charged, with a diameter of about 55 nm, and a zeta potential of -20 mV. In vitro study showed that the nanoparticles could easily be endocytosed by liver cancer cells (HepG2) and were well-accumulated in the liver by passive targeting. In vivo study proved the ability of DOX-MSN/COOH to inhibit the tumor growth and prolong the survival time of mice bearing hepatocellular carcinoma in situ, giving better results than free DOX. More importantly, histological examination showed no histopathological abnormalities of normal liver cells and heart cells after the administration of DOX-MSN/COOH, while the treatment with free DOX caused damage to those cells. In conclusion, DOX-MSN/COOH exhibited enhanced antitumor efficacy as well as reduced side effects for liver cancer therapy.
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Xie
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Yuanguo
authors.initials: Y
authors.lastname: Xu
authors.affiliation: School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Shen
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Song
authors.initials: S
authors.lastname: Shen
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Yanru
authors.initials: Y
authors.lastname: Ge
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: xiejm391@sohu.com.
authors.forename: Jimin
authors.initials: J
authors.lastname: Xie
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008813:Mice, Inbred ICR; D008819:Mice, Nude; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D009374:Neoplasms, Experimental / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000008:administration & dosage / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013499:Surface Properties; D014018:Tissue Distribution
chemical_list: D004337:Drug Carriers; D012822:Silicon Dioxide; D004317:Doxorubicin
keywords: Antitumor; Biodistribution; Doxorubicin; Liver tumor in situ; Mesoporous silica nanoparticles; Survival rate