title: A point-of-care testing system with Love-wave sensor and immunogold staining enhancement for early detection of lung cancer.
abstract: It has been reported that detection of exhaled breath condensate (EBC) is available for studies of pulmonary diseases, especially lung disease. In order to detect lung cancer (LC) at early stage, a point-of-care testing system suitable for measurement of tumor markers in EBC is developed. The assay, based on gold nanoparticle sandwich immunoassay and subsequent gold staining, was performed on a Love-wave sensor packaged inside a chip cartridge. Benefit from high sensitivity of Love-wave sensor, oriented immobilization of coating antibodies and immunogold staining enhancement, the present immunosensor could provide a sensitive, specific and rapid measurement. Carcinoembryonic antigen (CEA), neuron specific enolase (NSE) and squamous cell carcinoma antigen (SCC) in EBC collected from 17 patients with LC and 13 healthy volunteers were detected by this system. Results were compared with commercial chemiluminescence immunoassay and showed high correlation between two methods. Additionally, it revealed significantly statistical differences existing between two groups of subjects. These results indicate that the present system is suitable for detection of tumor markers in EBC and could be used as assistant tools for early detection of LC.
authors.affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027, Hangzhou, China.
authors.forename: Yingchang
authors.initials: Y
authors.lastname: Zou
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: An
authors.forename: Chunxue
authors.initials: C
authors.lastname: Ran
authors.forename: Kejing
authors.initials: K
authors.lastname: Ying
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D000951:Antigens, Neoplasm / Q000378:metabolism*; D001681:Biological Assay / Q000295:instrumentation / Q000379:methods; D014408:Biomarkers, Tumor / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000175:diagnosis / Q000378:metabolism; D019095:Point-of-Care Systems
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor