title: Citrimicrobium luteum gen. nov., sp. nov., aerobic anoxygenic phototrophic bacterium isolated from the gut of a sea cucumber Stichopus japonicus.
abstract: A Gram-stain negative, yellow-pigmented, motile, pleomorphic bacterium, designated strain CBA4602(T), was isolated from the gut of the sea cucumber Stichopus japonicus, which was collected from Jeju Island in the Republic of Korea. In a phylogenetic analysis based on the 16S rRNA gene, strain CBA4602(T) belonged to the order Sphingomonadales in the class Alphaproteobacteria. The 16S rRNA gene sequence similarity between strain CBA4602(T) and 'Citromicrobium bathyomarinum' JF-1, the most closely related strain having nonvalidly published name, was 98.4%, followed by 95.2-96.7% identities with sequence of the other closest strains in the genus Erythrobacter. Strain CBA4602(T) had bacteriochlorophyll a and carotenoids. Strain CBA4602(T) grew in 0-10% (w/v) NaCl, at 10-42°C and pH 6.0-8.0, with optimal growth in 1-2% NaCl, at 30-37°C and pH 7.0. Strain CBA4602(T) was positive for catalase and oxidase activities and was able to hydrolyse gelatine and Tween 20 and 40, but not starch, Tween 80 or L-tyrosine. The G+C content of genomic DNA from strain CBA4602(T) was 68.0 mol% and Q-10 was the major detected isoprenoid quinone. The polar lipids were three unidentified phospholipids, three unidentified glycolipids, and two unidentified lipids. The dominant fatty acids were anteiso-C15:0, C16:0, anteiso-C17:0 and C18:0. As considering the current taxonomic status of the genus 'Citromicrobium' and polyphasic taxonomic analyses, strain CBA4602(T) represents a novel genus and species. The name Citrimicrobium luteum is proposed for the type strain CBA4602(T) (=KACC 17668(T) =JCM 19530(T)).
authors.affiliation: Department of Biology and Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul, 130-701, Republic of Korea.
authors.forename: Hong-Joo
authors.initials: HJ
authors.lastname: Jung
authors.forename: In-Tae
authors.initials: IT
authors.lastname: Cha
authors.forename: Kyung June
authors.initials: KJ
authors.lastname: Yim
authors.forename: Hye Seon
authors.initials: HS
authors.lastname: Song
authors.forename: Kichul
authors.initials: K
authors.lastname: Cho
authors.forename: Daekyung
authors.initials: D
authors.lastname: Kim
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Lee
authors.forename: Jae Kook
authors.initials: JK
authors.lastname: Lee
authors.forename: Myung-Ji
authors.initials: MJ
authors.lastname: Seo
authors.forename: Seong Woon
authors.initials: SW
authors.lastname: Roh
authors.forename: Sung-Jae
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D000332:Aerobiosis; D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000818:Animals; D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004798:Enzymes / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D041981:Gastrointestinal Tract / Q000382:microbiology; D006017:Glycolipids / Q000032:analysis; D008124:Locomotion; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D047528:Stichopus / Q000382:microbiology*; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004798:Enzymes; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D010860:Pigments, Biological; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride