title: Genetically-encoded biosensors for monitoring cellular stress in bioprocessing.
abstract: With the current wealth of transcriptomic data, it is possible to design genetically-encoded biosensors for the detection of stress responses and apply these to high-throughput bioprocess development and monitoring of cellular health. Such biosensors can sense extrinsic factors such as nutrient or oxygen deprivation and shear stress, as well as intrinsic stress factors like oxidative damage and unfolded protein accumulation. Alongside, there have been developments in biosensing hardware and software applicable to the field of genetically-encoded biosensors in the near future. This review discusses the current state-of-the-art in biosensors for monitoring cultures during biological manufacturing and the future challenges for the field. Connecting the individual achievements into a coherent whole will enable the application of genetically-encoded biosensors in industry.
authors.affiliation: Department of Life Sciences, Imperial College London, London SW7 2AZ, UK; Centre for Synthetic Biology and Innovation, Imperial College London, London SW7 2AZ, UK. Electronic address: k.polizzi@imperial.ac.uk.
authors.forename: Karen M
authors.initials: KM
authors.lastname: Polizzi
authors.affiliation: Department of Chemical Engineering and Chemical Technology, Imperial College London, London SW7 2AZ, UK.
authors.forename: Cleo
authors.initials: C
authors.lastname: Kontoravdi
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002470:Cell Survival; D005819:Genetic Markers; D006801:Humans; D015398:Signal Transduction; D012984:Software; D013312:Stress, Physiological
chemical_list: D005819:Genetic Markers