title: Surface-enhanced Raman spectroscopy for simultaneous sensitive detection of multiple microRNAs in lung cancer cells.
abstract: We develop circular exponential amplification reaction (EXPAR)-based surface-enhanced Raman spectroscopy (SERS) for simultaneous sensitive detection of multiple microRNAs in non-small cell lung cancer cells. This method possesses distinct advantages of excellent selectivity and high sensitivity with a detection limit of as low as 0.5 fM, which has improved by as much as 6 orders of magnitude as compared with the previously reported SERS-based direct assay.
authors.affiliation: Single-Molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Guangdong 518055, China. zhangcy@siat.ac.cn.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Ye
authors.forename: Juan
authors.initials: J
authors.lastname: Hu
authors.forename: Li
authors.initials: L
authors.lastname: Liang
authors.forename: Chun-yang
authors.initials: CY
authors.lastname: Zhang
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D045744:Cell Line, Tumor; D006801:Humans; D057230:Limit of Detection; D008168:Lung / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics*; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D035683:MicroRNAs