title: Development of the excimer probe responsible for DNA target bearing the silylated pyrenes at base moiety.
abstract: For the development of the excimer probe responsible for DNA target, the deoxyuridine phosphoramidite derivative bearing the silylated pyrene attached at the C-5 position was prepared and incorporated into oligonucleotides. The modified oligonucleotides showed the excimer emission in the absence of the target DNA, on the other hands, the excimer emission was quenched in the presence of the target DNA. For the utilization of the fluorescence behavior, the novel molecular beacon probe containing the silylated pyrene-modified nucleoside at the stem region was designed and the fluorescence property of the probe found to show the responsibility for DNA target.
authors.affiliation: Division of Molecular Science, Graduate of Science and Technology, Gunma University, Tenjincho 1-5-1, Kiryu, Gunma 376-8515, Japan. Electronic address: moriguchi@gunma-u.ac.jp.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Moriguchi
authors.affiliation: Division of Molecular Science, Graduate of Science and Technology, Gunma University, Tenjincho 1-5-1, Kiryu, Gunma 376-8515, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ichimura
authors.affiliation: Division of Molecular Science, Graduate of Science and Technology, Gunma University, Tenjincho 1-5-1, Kiryu, Gunma 376-8515, Japan.
authors.forename: Mitsuhisa
authors.initials: M
authors.lastname: Kato
authors.affiliation: Division of Molecular Science, Graduate of Science and Technology, Gunma University, Tenjincho 1-5-1, Kiryu, Gunma 376-8515, Japan.
authors.forename: Kenya
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: Division of Molecular Science, Graduate of Science and Technology, Gunma University, Tenjincho 1-5-1, Kiryu, Gunma 376-8515, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takahashi
authors.affiliation: Division of Molecular Science, Graduate of Science and Technology, Gunma University, Tenjincho 1-5-1, Kiryu, Gunma 376-8515, Japan. Electronic address: sinozuka@chem-bio.gunma-u.ac.jp.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozuka
mesh_terms: D004247:DNA / Q000032:analysis*; D003857:Deoxyuridine / Q000737:chemistry*; D015195:Drug Design; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D009943:Organophosphorus Compounds; D011721:Pyrenes; C434331:phosphoramidite; D004247:DNA; D003857:Deoxyuridine
keywords: C-5 modified deoxyuridine; Excimer emission; Fluorescent DNA; Molecular beacon probe; Silylated pyrene