title: Octacalcium phosphate ceramics combined with gingiva-derived stromal cells for engineered functional bone grafts.
abstract: Biocompatible ceramic fillers are capable of sustaining bone formation in the proper environment. The major drawback of these scaffolding materials is the absence of osteoinductivity. To overcome this limitation, bioengineered scaffolds combine osteoconductive components (biomaterials) with osteogenic features such as cells and growth factors. The bone marrow mesenchymal stromal cells (BMMSCs) and the β-tricalcium phosphate (β-TCP) are well-known and characterized in this regard. The present study was conducted to compare the properties of novel octacalcium phosphate ceramic (OCP) granules with β-TCP (Cerasorb(®)), gingiva-derived mesenchymal stromal cells (GMSCs) properties with the BMMSCs and osteogenic and angiogenic properties of a bioengineered composite based on OCP granules and the GMSCs. This study demonstrates that GMSCs and BMMSСs have a similar osteogenic capacity. The usage of OCP ceramic granules in combination with BMMSCs/GMSCs significantly affects the osteo- and angiogenesis in bone grafts of ectopic models.
authors.affiliation: Human Stem Cells Institute, Moscow, Russia.
authors.forename: Vadim L
authors.initials: VL
authors.lastname: Zorin
authors.forename: Vladimir S
authors.initials: VS
authors.lastname: Komlev
authors.forename: Alla I
authors.initials: AI
authors.lastname: Zorina
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Khromova
authors.forename: Elena V
authors.initials: EV
authors.lastname: Solovieva
authors.forename: Alexander Yu
authors.initials: AY
authors.lastname: Fedotov
authors.forename: Ilya I
authors.initials: II
authors.lastname: Eremin
authors.forename: Pavel B
authors.initials: PB
authors.lastname: Kopnin
mesh_terms: D050156:Adipogenesis; D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry* / Q000633:toxicity; D002130:Calcium Phosphates / Q000737:chemistry* / Q000633:toxicity; D002454:Cell Differentiation; D049109:Cell Proliferation; D002516:Ceramics / Q000737:chemistry* / Q000633:toxicity; D020219:Chondrogenesis; D005260:Female; D005881:Gingiva / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D018919:Neovascularization, Physiologic; D010012:Osteogenesis; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; C022045:octacalcium phosphate