title: Phase-optimized chip-based liquid chromatography.
abstract: In this contribution we introduce phase-optimized columns for highly efficient liquid chromatographic separations in microfluidic glass chips. In phase-optimized liquid chromatography the selectivity and geometry of the stationary phase are precisely adjusted to provide an optimal separation of a mixture of interest. The separation of nine polycyclic aromatic hydrocarbons under reversed-phase conditions was investigated. Standard HPLC was utilized to explore the retention parameters of each analyte on a set of five commercially available stationary phases. From these experiments the properties of an optimal on-chip column were calculated assuming a zero-void-volume performance for the chip chromatography. A phase-optimized on-chip column only 30 mm long provided baseline resolution of all signals within 4 min. The separation performance of a chip column comprising various stationary phases can be precisely predicted by a set of traditional HPLC experiments. The approach has great potential for the directed development of tailor-made chromatography chips for specific applications.
authors.affiliation: Institute of Analytical Chemistry, University of Leipzig, Linnéstraße 3, 04103, Leipzig, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Thürmann
authors.forename: D
authors.initials: D
authors.lastname: Belder
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000295:instrumentation*; D056148:Chromatography, Reverse-Phase / Q000191:economics / Q000295:instrumentation*; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000295:instrumentation*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons