title: Preparative isolation of seven diterpenoid alkaloids from Aconitum coreanum by pH-zone-refining counter-current chromatography.
abstract: The aim of this paper was to seek an efficient method to preparative separation of alkaloid compounds from Aconitum coreanum (Guanbaifu), a well-known traditional Chinese medicinal plant for heart disease. Seven alkaloid compounds were successfully purified by pH-zone-refining counter-current chromatography with two-phase solvent system of petroleum ether-ethyl acetate-methanol-water (5:5:1:9, v/v/v/v), 10 mM triethylamine in upper phase and 10 mM hydrochloric acid in lower phase. From 3.5 g of crude extract, 356 mg of Guanfu base I, 578 mg of Guanfu base A, 74 mg of atisine, 94 mg of Guanfu base F, 423 mg of Guanfu base G, 67 mg of Guanfu base R and 154 mg of Guanfu base P were obtained with the purity of 96.40%, 97.2%, 97.5%, 98.1%, 98.9%, 98.3% and 98.4%. Their chemical structures were identified by TOF-MS and 1H-NMR. This study indicated that pH-zone-refining counter-current chromatography was an efficient method for separating the kind of alkaloids with low absorbance values.
authors.affiliation: College of Chinese Mareria Medica, Beijing University of Chinese Medicine, Beijing 100102, China. wxyph.d@163.com.
authors.forename: Xueyong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China. shuxikai5@163.com.
authors.forename: Xikai
authors.initials: X
authors.lastname: Shu
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China. wangx@sdas.org.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China. yujinqian87528@126.com.
authors.forename: Jinqian
authors.initials: J
authors.lastname: Yu
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China. gaoaixiangjf@163.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Jing
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D003377:Countercurrent Distribution; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000737:chemistry / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D012997:Solvents / Q000737:chemistry
chemical_list: D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D006576:Heterocyclic Compounds, 4 or More Rings; D012997:Solvents; C572618:guanfu base G; C040615:Guan-fu base A