title: Antiviral potency and functional analysis of tetherin orthologues encoded by horse and donkey.
abstract: BACKGROUND
  Tetherin is an interferon-inducible host cell factor that blocks the viral particle release of the enveloped viruses. Most knowledge regarding the interaction between tetherin and viruses has been obtained using the primate lentiviral system. However, much less is known about the functional roles of tetherin on other lentiviruses. Equine infectious anemia virus (EIAV) is an important macrophage-tropic lentivirus that has been widely used as a practical model for investigating the evolution of the host-virus relationship. The host range of EIAV is reported to include all members of the Equidae family. However, EIAV has different clinical responses in horse and donkey. It's intriguing to investigate the similarities and differences between the tetherin orthologues encoded by horse and donkey.
  
  
  RESULTS
  We report here that there are two equine tetherin orthologues. Compared to horse tetherin, there are three valine amino acid deletions within the transmembrane domain and three distinct mutations within the ectodomain of donkey tetherin. However, the antiviral activity of donkey tetherin was not affected by amino acid deletion or substitution. In addition, both tetherin orthologues encoded by horse and donkey are similarly sensitive to EIAV Env protein, and equally activate NF-κB signaling.
  
  
  CONCLUSIONS
  Our data suggest that both tetherin orthologues encoded by horse and donkey showed similar antiviral activities and abilities to induce NF-κB signaling. In addition, the phenomenon about the differential responses of horses and donkeys to infection with EIAV was not related with the differences in the structure of the corresponding tetherin orthologues.
authors.forename: Xin
authors.initials: X
authors.lastname: Yin
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Guo
authors.forename: Qinyong
authors.initials: Q
authors.lastname: Gu
authors.forename: Xingliang
authors.initials: X
authors.lastname: Wu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, China. weiiping@163.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018397:Equidae / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D004860:Infectious Anemia Virus, Equine / Q000187:drug effects*; D008264:Macrophages / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009154:Mutation; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D020033:Protein Isoforms; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D000998:Antiviral Agents; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D020033:Protein Isoforms