title: Anti-inflammatory and membrane-stabilizing properties of two semisynthetic derivatives of oleanolic acid.
abstract: Acetylation and methylation semisynthesis of oleanolic acid (OA) isolated from Syzygium aromaticum L. yielded two compounds: 3-acetoxyoleanolic acid (3-AOA) and 3-acetoxy, 28-methylester oleanolic acid (3-A,28-MOA). Anti-inflammatory properties of these compounds were assessed using the serotonin and fresh egg albumin-induced inflammatory test models in male Wistar rats weighing 250-300 g. Furthermore, erythrocyte membrane-stabilizing property of these compounds was evaluated in the heat- and hypotonicity-induced in vitro hemolysis test models. The two semisynthetic compounds significantly (p < 0.05) inhibited albumin-induced inflammation better than OA and indomethacin from 1-5 h post administration. Both compounds were membrane stabilizing in heat-induced hemolysis test while only 3-AOA showed membrane-stabilizing effects in a hypotonic milieu. Semisynthesis of OA yielded two compounds which had better in vivo anti-inflammatory and in vitro membrane-stabilizing properties.
authors.affiliation: Department of Zoology, Faculty of Science, Engineering & Technology, PB X1, Mthatha, 5117, Eastern Cape Province, South Africa, bnkehchungag@wsu.ac.za.
authors.forename: Benedicta N
authors.initials: BN
authors.lastname: Nkeh-Chungag
authors.forename: Opeoluwa O
authors.initials: OO
authors.lastname: Oyedeji
authors.forename: Adebola O
authors.initials: AO
authors.lastname: Oyedeji
authors.forename: Eugene J
authors.initials: EJ
authors.lastname: Ndebia
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D006461:Hemolysis / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008297:Male; D009828:Oleanolic Acid / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D027842:Syzygium
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D009828:Oleanolic Acid