title: Different effects of two newly-isolated probiotic Lactobacillus plantarum 15HN and Lactococcus lactis subsp. Lactis 44Lac strains from traditional dairy products on cancer cell lines.
abstract: Lactobacillus and Lactococcus strains isolated from food products can be introduced as probiotics because of their health-promoting characteristics and non-pathogenic nature. This study aims to perform the isolation, molecular identification, and probiotic characterization of Lactobacillus and Lactococcus strains from traditional Iranian dairy products. Primary probiotic assessments indicated high tolerance to low pH and high bile salt conditions, high anti-pathogenic activities, and susceptibility to high consumption antibiotics, thus proving that both strains possess probiotic potential. Cytotoxicity assessments were used to analyze the effects of the secreted metabolite on different cancer cell lines, including HT29, AGS, MCF-7, and HeLa, as well as a normal human cell line (HUVEC). Results showed acceptable cytotoxic properties for secreted metabolites (40 μg/ml dry weight) of Lactococcus lactis subsp. Lactis 44Lac. Such performance was similar to that of Taxol against all of the treated cancer cell lines; however, the strain exhibited no toxicity on the normal cell line. Cytotoxic assessments through flow cytometry and fluorescent microscopy demonstrated that apoptosis is the main cytotoxic mechanism for secreted metabolites of L. lactis subsp. Lactis 44Lac. By contrast, the effects of protease-treated metabolites on the AGS cell line verified the protein nature of anti-cancer metabolites. However, precise characterizations and in vitro/in vivo investigations on purified proteins should be conducted before these metabolites are introduced as potential anti-cancer therapeutics.
authors.affiliation: Institute of Biosciences, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Babak
authors.initials: B
authors.lastname: Haghshenas
authors.affiliation: Chemical and Environmental Engineering Department, Faculty of Engineering, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: nhafizah@upm.edu.my.
authors.forename: Norhafizah
authors.initials: N
authors.lastname: Abdullah
authors.affiliation: Institute of Biosciences, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Yousef
authors.initials: Y
authors.lastname: Nami
authors.affiliation: Chemical and Environmental Engineering Department, Faculty of Engineering, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Dayang
authors.initials: D
authors.lastname: Radiah
authors.affiliation: Institute of Biosciences, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Rozita
authors.initials: R
authors.lastname: Rosli
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: Yarikhosroushahia@tbzmed.ac.ir.
authors.forename: Ahmad Yari
authors.initials: AY
authors.lastname: Khosroushahi
mesh_terms: D000143:Acids / Q000633:toxicity; D000970:Antineoplastic Agents / Q000494:pharmacology; D001647:Bile Acids and Salts / Q000378:metabolism; D001688:Biological Products / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003611:Dairy Products / Q000382:microbiology*; D004361:Drug Tolerance; D005434:Flow Cytometry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007492:Iran; D048191:Lactiplantibacillus plantarum / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D013294:Lactococcus lactis / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D008856:Microscopy, Fluorescence; D019936:Probiotics
chemical_list: D000143:Acids; D000970:Antineoplastic Agents; D001647:Bile Acids and Salts; D001688:Biological Products
keywords: Antibacterial; Antibiotic susceptibility; Anticancer; Apoptosis