title: Photoinduced electron transfer (PET) based label-free aptasensor for platelet-derived growth factor-BB and its logic gate application.
abstract: Platelet-derived growth factor-BB (PDGF-BB) is often overexpressed in human malignant tumors as an indicator for tumor angiogenesis. Here by the photoinduced electron transfer (PET) between DNA-Ag fluorescent nanoclusters (NCs) and G-quadruplex/hemin complexes, we present a sensitive label-free fluorescent sensor for PDGF-BB. In the presence of PDGF-BB, the specific conjugation with its aptamer induced the conformational change of the duplex-like DNA sequence, releasing the G-quadruplex sequence part. Then in the presence of hemin and K(+), the horseradish peroxidase mimicking DNAzyme (HRP-DNAzyme) was formed. With the electron transfer between the DNA-Ag NCs to the hemin Fe (III) center of HRP-DNAzyme, the PET occurred with a decrease in the fluorescence intensity of the DNA-Ag NCs. The detection performance such as selectivity, linear dynamic range, sensitivity, and the quenching capability of HRP-DNAzyme were estimated. The detection range for PDGF-BB is from 5×10(-13) to 1×10(-8) M and the detection limit is 1×10(-13) M. The experimental results confirmed that the novel fluorescent aptasensor possessed a good sensitivity and high selectivity for PDGF-BB. In addition, the developed probe is nontoxic, label-free only involving one-step hybridization without sophisticated fabrication process. Furthermore, based on this quenching mode occurred by PDGF-BB and hemin, using PDGF-BB and hemin as inputs and the fluorescence signal as an output, a logic gate has been fabricated.
authors.affiliation: Anhui Key Laboratory of Chem-Biosensing, College of Chemistry and Materials Science, Center for Nanoscience and Nanotechnology, Anhui Normal University, Wuhu 241000, PR China; State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China. Electronic address: wangyuz@mail.ahnu.edu.cn.
authors.forename: Guangfeng
authors.initials: G
authors.lastname: Wang
authors.affiliation: Anhui Key Laboratory of Chem-Biosensing, College of Chemistry and Materials Science, Center for Nanoscience and Nanotechnology, Anhui Normal University, Wuhu 241000, PR China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Anhui Key Laboratory of Chem-Biosensing, College of Chemistry and Materials Science, Center for Nanoscience and Nanotechnology, Anhui Normal University, Wuhu 241000, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: Anhui Key Laboratory of Chem-Biosensing, College of Chemistry and Materials Science, Center for Nanoscience and Nanotechnology, Anhui Normal University, Wuhu 241000, PR China; State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China. Electronic address: xjzhang@mail.ahnu.edu.cn.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics; D000077214:Becaplermin; D015374:Biosensing Techniques / Q000295:instrumentation*; D021881:DNA, Catalytic / Q000737:chemistry*; D004579:Electron Transport / Q000528:radiation effects; D004867:Equipment Design; D019544:Equipment Failure Analysis; D054856:G-Quadruplexes; D006427:Hemin / Q000737:chemistry* / Q000235:genetics; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry; D020574:Proto-Oncogene Proteins c-sis / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation; D012834:Silver / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D013194:Staining and Labeling
chemical_list: D052157:Aptamers, Nucleotide; D021881:DNA, Catalytic; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D012834:Silver; D006427:Hemin
keywords: Hemin/G-quadruplex complex; Logic gate; PDGF-BB; Photoinduced electron transfer; Silver nanoclusters