title: Identification of Pork Contamination in Meatballs of Indonesia Local Market Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis.
abstract: This research applied and evaluated a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using cytochrome b gene to detect pork contamination in meatballs from local markets in Surabaya and Yogyakarta regions, Indonesia. To confirm the effectiveness and specificity of this fragment, thirty nine DNA samples from different meatball shops were isolated and amplified, and then the PCR amplicon was digested by BseDI restriction enzyme to detect the presence of pork in meatballs. BseDI restriction enzyme was able to cleave porcine cytochrome b gene into two fragments (131 bp and 228 bp). Testing the meatballs from the local market showed that nine of twenty meatball shops in Yogyakarta region were detected to have pork contamination, but there was no pork contamination in meatball shops in Surabaya region. In conclusion, specific PCR amplification of cytochrome b gen and cleaved by BseDI restriction enzymes seems to be a powerful technique for the identification of pork presence in meatball because of its simplicity, specificity and sensitivity. Furthermore, pork contamination intended for commercial products of sausage, nugget, steak and meat burger can be checked. The procedure is also much cheaper than other methods based on PCR, immunodiffusion and other techniques that need expensive equipment.
authors.affiliation: Halal Products Research Center, Gadjah Mada University, Yogyakarta 55281, Indonesia .
authors.forename: Yuny
authors.initials: Y
authors.lastname: Erwanto
authors.affiliation: Halal Products Research Center, Gadjah Mada University, Yogyakarta 55281, Indonesia .
authors.forename: Mohammad Zainal
authors.initials: MZ
authors.lastname: Abidin
authors.affiliation: Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta 55281, Indonesia .
authors.forename: Eko Yasin Prasetyo Muslim
authors.initials: EY
authors.lastname: Sugiyono
authors.affiliation: Halal Products Research Center, Gadjah Mada University, Yogyakarta 55281, Indonesia . ; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta 55281, Indonesia .
authors.forename: Abdul
authors.initials: A
authors.lastname: Rohman
keywords: Detection; Meatballs; Polymerase Chain Reaction; Pork Contamination