title: The class III ribonucleotide reductase from Neisseria bacilliformis can utilize thioredoxin as a reductant.
abstract: The class III anaerobic ribonucleotide reductases (RNRs) studied to date couple the reduction of ribonucleotides to deoxynucleotides with the oxidation of formate to CO2. Here we report the cloning and heterologous expression of the Neisseria bacilliformis class III RNR and show that it can catalyze nucleotide reduction using the ubiquitous thioredoxin/thioredoxin reductase/NADPH system. We present a structural model based on a crystal structure of the homologous Thermotoga maritima class III RNR, showing its architecture and the position of conserved residues in the active site. Phylogenetic studies suggest that this form of class III RNR is present in bacteria and archaea that carry out diverse types of anaerobic metabolism.
authors.affiliation: Departments of Chemistry and yifwei@mit.edu cdrennan@mit.edu stubbe@mit.edu.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Departments of Chemistry and.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Funk
authors.affiliation: Departments of Chemistry and.
authors.forename: Leonardo A
authors.initials: LA
authors.lastname: Rosado
authors.affiliation: Departments of Chemistry and.
authors.forename: Jiyeon
authors.initials: J
authors.lastname: Baek
authors.affiliation: Departments of Chemistry and Biology, and Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139 yifwei@mit.edu cdrennan@mit.edu stubbe@mit.edu.
authors.forename: Catherine L
authors.initials: CL
authors.lastname: Drennan
authors.affiliation: Departments of Chemistry and Biology, and yifwei@mit.edu cdrennan@mit.edu stubbe@mit.edu.
authors.forename: JoAnne
authors.initials: J
authors.lastname: Stubbe
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D055162:Biocatalysis; D020134:Catalytic Domain; D019295:Computational Biology; D018360:Crystallography, X-Ray; D003570:Cytidine Triphosphate / Q000378:metabolism; D003596:Cytosine / Q000378:metabolism; D004220:Disulfides / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D008954:Models, Biological; D009249:NADP; D009343:Neisseria / Q000201:enzymology*; D010084:Oxidation-Reduction; D019163:Reducing Agents / Q000378:metabolism*; D012264:Ribonucleotide Reductases / Q000737:chemistry / Q000378:metabolism*; D020124:Thermotoga maritima / Q000201:enzymology; D013879:Thioredoxins / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000596:Amino Acids; D004220:Disulfides; D019163:Reducing Agents; D013879:Thioredoxins; D009249:NADP; D003570:Cytidine Triphosphate; D003596:Cytosine; D012264:Ribonucleotide Reductases