title: Fucoidan promotes the reconstruction of skin equivalents.
abstract: In this study we investigated the effects of fucoidan on the proliferation of fibroblasts and the reconstruction of a skin equivalent (SE). Fucoidan significantly stimulated the proliferation of CCD-25Sk human fibroblasts and Western blot analysis demonstrated that fucoidan markedly increased the expression of cyclin D1 and decreased the expression of p27. Fucoidan was used to reconstruct SE. Immunohistochemical staining showed that the addition of fucoidan to dermal equivalents increased expression of proliferating cell nuclear antigen (PCNA) and p63. In addition, expression of α6-integrin was significantly increased by fucoidan, whereas expression of β1-integrin, type 1 collagen, elastin, fibronectin did not markedly change. These results suggest that fucoidan has positive effects on epidermal reconstruction and will therefore be beneficial in the reconstruction of SE.
authors.affiliation: Department of Biochemistry, Chung-Ang University College of Medicine, Seoul 156-756, Korea.
authors.forename: Yu Seok
authors.initials: YS
authors.lastname: Song
authors.affiliation: Department of Biochemistry, Chung-Ang University College of Medicine, Seoul 156-756, Korea.
authors.forename: Hailan
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Biochemistry, Chung-Ang University College of Medicine, Seoul 156-756, Korea.
authors.forename: Marie Carmel
authors.initials: MC
authors.lastname: Balcos
authors.affiliation: Department of Biochemistry, Chung-Ang University College of Medicine, Seoul 156-756, Korea.
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Yun
authors.affiliation: Department of Biochemistry, Chung-Ang University College of Medicine, Seoul 156-756, Korea.
authors.forename: Kwang Jin
authors.initials: KJ
authors.lastname: Baek
authors.affiliation: Department of Biochemistry, Chung-Ang University College of Medicine, Seoul 156-756, Korea.
authors.forename: Nyoun Soo
authors.initials: NS
authors.lastname: Kwon
authors.affiliation: Department of Dermatology, Seoul National University Bundang Hospital, Seongnam 463-707, Korea.
authors.forename: Hye-Ryung
authors.initials: HR
authors.lastname: Choi
authors.affiliation: Department of Dermatology, Seoul National University Bundang Hospital, Seongnam 463-707, Korea.
authors.forename: Kyoung-Chan
authors.initials: KC
authors.lastname: Park
authors.affiliation: Department of Biochemistry, Chung-Ang University College of Medicine, Seoul 156-756, Korea.
authors.forename: Dong-Seok
authors.initials: DS
authors.lastname: Kim
keywords: Fibroblasts; Fucoidan; Proliferation; Skin equivalent