title: Plasmonic nanosensors for simultaneous quantification of multiple protein-protein binding affinities.
abstract: Most of current techniques used for the quantification of protein-protein interactions require the analysis of one pair of binding partners at a time. Herein we present a label-free, simple, fast, and cost-effective route to characterize binding affinities between multiple macromolecular partners simultaneously, using optical dark-field spectroscopy and individual protein-functionalized gold nanorods as sensing elements. Our NanoSPR method could easily become a simple and standard tool in biological, biochemical, and medical laboratories.
authors.affiliation: Institute of Physical Chemistry, University of Mainz , Duesbergweg 10-14, D-55128 Mainz, Germany.
authors.forename: Rubén
authors.initials: R
authors.lastname: Ahijado-Guzmán
authors.forename: Janak
authors.initials: J
authors.lastname: Prasad
authors.forename: Christina
authors.initials: C
authors.lastname: Rosman
authors.forename: Andreas
authors.initials: A
authors.lastname: Henkel
authors.forename: Lydia
authors.initials: L
authors.lastname: Tome
authors.forename: Dirk
authors.initials: D
authors.lastname: Schneider
authors.forename: Germán
authors.initials: G
authors.lastname: Rivas
authors.forename: Carsten
authors.initials: C
authors.lastname: Sönnichsen
mesh_terms: D001419:Bacteria / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D008958:Models, Molecular; D043942:Nanotubes / Q000737:chemistry*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000191:economics / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000191:economics / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria; D006046:Gold
keywords: FtsZ; NanoSPR; multiple protein−protein interactions; optical dark-field spectroscopy; plasmonic nanosensors