title: miR-339-5p inhibits alcohol-induced brain inflammation through regulating NF-κB pathway.
abstract: Alcohol-induced neuroinflammation is mediated by the innate immunesystem. Pro-inflammatory responses to alcohol are modulated by miRNAs. The miRNA miR-339-5p has previously been found to be upregulated in alcohol-induced neuroinflammation. However, little has been elucidated on the regulatory functions of this miRNA in alcohol-induced neuroinflammation. We investigated the function of miR-339-5p in alcohol exposed brain tissue and isolated microglial cells using ex vivo and in vitro techniques. Our results show that alcohol induces transcription of miR 339-5p, IL-6, IL-1β and TNF-α in mouse brain tissue and isolated microglial cells by activating NF-κB. Alcohol activation of NF-κB allows for nuclear translocation of the NF-κB subunit p65 and expression of pro-inflammatory mediators. miR-339-5p inhibited expression of these pro-inflammatory factors through the NF-κB pathway by abolishing IKK-β and IKK-ε activity.
authors.affiliation: Department of Neurology, The First Affiliated School of Harbin Medical University, Harbin 150001, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Heilongjiang Provincial Hospital, Harbin 150036, China.
authors.forename: Guangkuan
authors.initials: G
authors.lastname: Wei
authors.affiliation: Department of Laboratory, Heilongjiang Provincial Hospital, Harbin 150036, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Di
authors.affiliation: Department of Neurology, The First Affiliated School of Harbin Medical University, Harbin 150001, China. Electronic address: zhaoqingjie2013@163.com.
authors.forename: Qingjie
authors.initials: Q
authors.lastname: Zhao
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D004660:Encephalitis / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000431:Ethanol / Q000494:pharmacology*; D005786:Gene Expression Regulation; D051550:I-kappa B Kinase / Q000235:genetics* / Q000378:metabolism; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000378:metabolism; D015850:Interleukin-6 / Q000096:biosynthesis / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D017628:Microglia / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008969:Molecular Sequence Data; D061251:Primary Cell Culture; D021381:Protein Transport; D015398:Signal Transduction; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D015850:Interleukin-6; D035683:MicroRNAs; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D000431:Ethanol; D051550:I-kappa B Kinase; C496562:Ikbkb protein, mouse
keywords: Alcohol; Brain-inflammation; MicroRNA; NF-κB; miR-339-5p