title: Role of the TGFβ/p65 pathway in tanshinone ⅡA-treated HBZY‑1 cells.
abstract: Tanshinone ⅡA (TⅡA) is widely used for the treatment of a number human diseases, including diabetic nephropathy (DN) (1). The present study was performed to examine the role of the transforming growth factor β (TGFβ)/p65 pathway under TⅡA treatment in a glomerular mesangial cell model of DN. Firstly, it was identified that TⅡA inhibited the proliferation of HBZY‑1 cells, while simultaneously suppressing the expression of TGFβ and p65. In addition, glucose-induced HBZY‑1 cells were treated with TⅡA, si‑TGFβ and si‑p65. The results revealed that si‑TGFβ or si‑p65 were able to inhibit the proliferation of HBZY‑1 cells as well. Finally, the expression of TGFβ and p65 in a rat model of DN treated with TⅡA was detected. The results demonstrated that renal hypertrophy and 24 h urinary protein excretion were ameliorated in TⅡA-treated rats with DN. Furthermore, it was revealed that the protein levels of TGFβ and p65 were decreased in the DN rats following TⅡA treatment. In conclusion, the present study demonstrated that TGFβ and p65 were activated by TⅡA in HBZY‑1 cells. In addition, the expression of TGFβ and of p65 was downregulated in rats with DN treated with TⅡA.
authors.affiliation: Division of Nephrology, The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong 510405, P.R. China.
authors.forename: Gangyi
authors.initials: G
authors.lastname: Chen
authors.affiliation: BGI‑Shenzhen, Shenzhen, Guangdong 518083, P.R. China.
authors.forename: Xingju
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Shenzhen Engineering Laboratory for Genomics‑Assisted Animal Breeding, BGI‑Shenzhen, Shenzhen, Guangdong 518083, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Internal Medicine, Guangdong Second Provincial Traditional Chinese Medicine Hospital, Guangzhou, Guangdong 510095, P.R. China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Division of Nephrology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Division of Nephrology, The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong 510405, P.R. China.
authors.forename: Shuifu
authors.initials: S
authors.lastname: Tang
mesh_terms: D045784:Abietanes / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000652:urine; D003928:Diabetic Nephropathies / Q000188:drug therapy* / Q000209:etiology / Q000652:urine; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism*; D029543:Nucleocytoplasmic Transport Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D045784:Abietanes; D009363:Neoplasm Proteins; D029543:Nucleocytoplasmic Transport Proteins; D016212:Transforming Growth Factor beta; C063447:p65 oncofetal mRNA transport protein, rat; C021751:tanshinone