title: Preventive effects of dexmedetomidine on the liver in a rat model of acid-induced acute lung injury.
abstract: The aim of this study was to examine whether dexmedetomidine improves acute liver injury in a rat model. Twenty-eight male Wistar albino rats weighing 300-350 g were allocated randomly to four groups. In group 1, normal saline (NS) was injected into the lungs and rats were allowed to breathe spontaneously. In group 2, rats received standard ventilation (SV) in addition to NS. In group 3, hydrochloric acid was injected into the lungs and rats received SV. In group 4, rats received SV and 100 µg/kg intraperitoneal dexmedetomidine before intratracheal HCl instillation. Blood samples and liver tissue specimens were examined by biochemical, histopathological, and immunohistochemical methods. Acute lung injury (ALI) was found to be associated with increased malondialdehyde (MDA), total oxidant activity (TOA), oxidative stress index (OSI), and decreased total antioxidant capacity (TAC). Significantly decreased MDA, TOA, and OSI levels and significantly increased TAC levels were found with dexmedetomidine injection in group 4 (P < 0.05). The highest histologic injury scores were detected in group 3. Enhanced hepatic vascular endothelial growth factor (VEGF) expression and reduced CD68 expression were found in dexmedetomidine group compared with the group 3. In conclusion, the presented data provide the first evidence that dexmedetomidine has a protective effect on experimental liver injury induced by ALI.
authors.affiliation: Department of Pediatrics, Dicle University Medical School, Diyarbakir, Turkey.
authors.forename: Velat
authors.initials: V
authors.lastname: Sen
authors.affiliation: Department of Anesthesiology, Dicle University Medical School, Diyarbakir, Turkey.
authors.forename: Abdulmenap
authors.initials: A
authors.lastname: Güzel
authors.affiliation: Department of Pulmonology, Dicle University Medical School, Diyarbakir, Turkey.
authors.forename: Hadice Selimoğlu
authors.initials: HS
authors.lastname: Şen
authors.affiliation: Department of Pediatrics, Dicle University Medical School, Diyarbakir, Turkey.
authors.forename: Aydın
authors.initials: A
authors.lastname: Ece
authors.affiliation: Department of Pediatrics, Dicle University Medical School, Diyarbakir, Turkey.
authors.forename: Unal
authors.initials: U
authors.lastname: Uluca
authors.affiliation: Department of Histology and Embryology, Dicle University Medical School, Diyarbakir, Turkey.
authors.forename: Sevda
authors.initials: S
authors.lastname: Söker
authors.affiliation: Department of Anesthesiology, Dicle University Medical School, Diyarbakir, Turkey.
authors.forename: Erdal
authors.initials: E
authors.lastname: Doğan
authors.affiliation: Department of Biochemistry, Dicle University Medical School, Diyarbakir, Turkey.
authors.forename: İbrahim
authors.initials: İ
authors.lastname: Kaplan
authors.affiliation: Department of Histology and Embryology, Dicle University Medical School, Diyarbakir, Turkey.
authors.forename: Engin
authors.initials: E
authors.lastname: Deveci
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D020927:Dexmedetomidine / Q000008:administration & dosage*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D006851:Hydrochloric Acid / Q000633:toxicity; D008099:Liver / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
chemical_list: D000975:Antioxidants; D042461:Vascular Endothelial Growth Factor A; D008315:Malondialdehyde; D020927:Dexmedetomidine; D006851:Hydrochloric Acid