title: Alkaloids from Mahonia bealei posses anti-H⁺/K⁺-ATPase and anti-gastrin effects on pyloric ligation-induced gastric ulcer in rats.
abstract: The purpose of this study was to investigate the underlying mechanism(s) of the total alkaloids (TA) from Mahonia bealei in treating pyloric ligation-induced gastric ulcers in rats. Animals were sacrificed after 19 h of the ligation. Gastric acid, peptic activities, mucin levels, H(+)/K(+)-ATPase activities and the gastrin level were analyzed. To improve the accuracy of the observations, IPP 6.0 software was introduced to measure the area of ulcer. TA (18.56 mg/kg/day, i.g.) showed an antiulcer effect by significantly decreasing the gastric ulcer areas (11.28 mm(2)) compared with model group (26.36 mm(2)). The TA ulcer inhibition ratio was 57.2%, compared with the effect of the positive control, omeprazole (62.96%). The results also showed that TA had a significant effect in inhibiting the release of H(+)/K(+)-ATPase, reducing the content of gastrin and decreasing gastric acidity on experimental animals. However, the TA had no significant effects on gastric mucus secretion and pepsin activity. Data indicated that TA had gastric ulcer protective effects by modulating the H(+)/K(+)-ATPase activity and gastrin level. TA has a potential to be developed as a pharmacological agent for the treatment of gastric ulcers.
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, JS 210009, China.
authors.forename: Su-Li
authors.initials: SL
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, JS 210009, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, JS 210000, China.
authors.forename: Xin
authors.initials: X
authors.lastname: He
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, JS 210009, China.
authors.forename: Run-Qi
authors.initials: RQ
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, JS 210009, China.
authors.forename: Yu-He
authors.initials: YH
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, JS 210009, China; Tang Center of Herbal Medicine and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL 60637, USA. Electronic address: zhangchunfeng67@163.com.
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Zhang
authors.affiliation: Tang Center of Herbal Medicine and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Chong-Zhi
authors.initials: CZ
authors.lastname: Wang
authors.affiliation: Tang Center of Herbal Medicine and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Chun-Su
authors.initials: CS
authors.lastname: Yuan
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D005744:Gastric Acid / Q000737:chemistry; D005755:Gastrins / Q000097:blood*; D017506:H(+)-K(+)-Exchanging ATPase / Q000378:metabolism; D008026:Ligation; D028143:Mahonia / Q000737:chemistry*; D008297:Male; D009077:Mucins / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000188:drug therapy*; D053719:Tandem Mass Spectrometry
chemical_list: D000470:Alkaloids; D000897:Anti-Ulcer Agents; D005755:Gastrins; D009077:Mucins; D017506:H(+)-K(+)-Exchanging ATPase
keywords: Alkaloid; Gastric ulcer; Gastrin; H(+)/K(+)-ATPase; Mahonia; Pyloric ligation