title: Microscopy assays for evaluation of mast cell migration and chemotaxis.
abstract: A better understanding of the molecular mechanisms leading to mast cell migration and chemotaxis is the long-term goal in mast cell research and is essential for comprehension of mast cell function in health and disease. Various techniques have been developed in recent decades for in vitro and in vivo assessment of mast cell motility and chemotaxis. In this chapter three microscopy assays facilitating real-time quantification of mast cell chemotaxis and migration are described, focusing on individual cell tracking and data analysis.
authors.affiliation: Laboratory of Signal Transduction Institute of Molecular Genetics, v.v.i., Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague 4, Czech Republic.
authors.forename: Monika
authors.initials: M
authors.lastname: Bambousková
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Hájková
authors.forename: Pavel
authors.initials: P
authors.lastname: Dráber
authors.forename: Petr
authors.initials: P
authors.lastname: Dráber
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D054443:Cell Migration Assays / Q000379:methods*; D002465:Cell Movement / Q000187:drug effects; D058948:Cell Tracking / Q000379:methods; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000494:pharmacology; D002633:Chemotaxis; D006801:Humans; D008407:Mast Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D008853:Microscopy / Q000379:methods*
chemical_list: D002630:Chemotactic Factors