title: Toward hyperpolarized molecular imaging of HIV: synthesis and longitudinal relaxation properties of (15) N-Azidothymidine.
abstract: Previously unreported (15) N labeled Azidothymidine (AZT) was prepared as an equimolar mixture of two isotopomers: 1-(15) N-AZT and 3-(15) N-AZT. Polarization decay of (15) N NMR signal was studied in high (9.4 T) and low (~50 mT) magnetic fields. (15) N T1 values were 45 ± 5 s (1-(15) N-AZT) and 37 ± 2 s (3-(15) N-AZT) at 9.4 T, and 140 ± 16 s (3-(15) N-AZT) at 50 mT. (15) N-AZT can be potentially (15) N hyperpolarized by several methods. These sufficiently long (15) N-AZT T1 values potentially enable hyperpolarized in vivo imaging of (15) N-AZT, because of the known favorable efficient (i.e., of the time scale shorter than the longest reported here (15) N T1 ) kinetics of uptake of injected AZT. Therefore, 3-(15) N-AZT can be potentially used for HIV molecular imaging using hyperpolarized magnetic resonance imaging.
authors.affiliation: Department of Radiology, Vanderbilt University Institute of Imaging Science (VUIIS), Nashville, TN, 37232, USA.
authors.forename: Roman V
authors.initials: RV
authors.lastname: Shchepin
authors.forename: Eduard Y
authors.initials: EY
authors.lastname: Chekmenev
mesh_terms: D019380:Anti-HIV Agents / Q000138:chemical synthesis* / Q000737:chemistry; D009590:Nitrogen Radioisotopes / Q000737:chemistry; D019275:Radiopharmaceuticals / Q000138:chemical synthesis* / Q000737:chemistry; D015215:Zidovudine / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D019380:Anti-HIV Agents; D009590:Nitrogen Radioisotopes; D019275:Radiopharmaceuticals; D015215:Zidovudine
keywords: 15N; AIDS; AZT; HIV; MRI; azidothymidine; contrast agent; hyperpolarization