title: Repair of canine medial orbital bone defects with miR-31-modified bone marrow mesenchymal stem cells.
abstract: OBJECTIVE
  To investigate the role of miR-31 genetically modified bone marrow mesenchymal stem cells (BMSCs) composited with porous β-tricalcium phosphate (β-TCP) scaffolds in repairing canine medial orbital wall defects.
  
  
  METHODS
  A circular bone defect (10 mm in diameter) was created on the canine medial orbital wall. After canine BMSCs were isolated and transfected with lentiviral vectors encoding miR-31, anti-miR-31 (anti-miR), and negative control (miR-Neg) in vitro, they were seeded onto porous β-TCP scaffolds and implanted to repair the orbital defects. Spiral computed tomography (CT) scans were conducted at 4 and 16 weeks after surgery. Micro-CT and histological analysis were performed at 16 weeks after surgery. The results were analyzed to evaluate the extent of bone repair.
  
  
  RESULTS
  Examination with CT revealed good recovery in the anti-miR group at 16 weeks after surgery. In addition, the micro-CT analysis showed that the bone mineral density and new bone volume increased in the anti-miR group and decreased in the miR-31 group compared with that in the miR-Neg group. Histologic analysis confirmed that the formation of new bone and extent of β-TCP degradation were enhanced in the anti-miR and attenuated in the miR-31 group. In situ hybridization and immunohistochemical analysis further confirmed the micro-CT findings.
  
  
  CONCLUSIONS
  The use of BMSCs with suppression of miR-31 expression combined with β-TCP scaffolds can efficiently repair medial orbital wall defects in dogs.
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Gu
authors.affiliation: Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Xianqun
authors.initials: X
authors.lastname: Fan
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D001861:Bone Regeneration / Q000235:genetics / Q000502:physiology; D002130:Calcium Phosphates / Q000494:pharmacology; D004195:Disease Models, Animal; D004285:Dogs; D049452:Green Fluorescent Proteins / Q000235:genetics; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D035683:MicroRNAs / Q000502:physiology*; D009915:Orbit / Q000000981:diagnostic imaging / Q000473:pathology*; D009916:Orbital Diseases / Q000000981:diagnostic imaging / Q000473:pathology / Q000628:therapy*; D010012:Osteogenesis / Q000235:genetics / Q000502:physiology; D051381:Rats; D054457:Tissue Scaffolds; D055114:X-Ray Microtomography
chemical_list: D002130:Calcium Phosphates; C570112:MIRN31 microRNA, rat; D035683:MicroRNAs; C485817:beta-tricalcium phosphate; D049452:Green Fluorescent Proteins
keywords: bone marrow mesenchymal stem cells; bone repair; miR-31; orbital bone defects; β-tricalcium phosphate