title: Cluster-determinant 36 (CD36) impacts on vitamin E postprandial response.
abstract: METHODS
  A single nucleotide polymorphism in the cluster determinant 36 (CD36) gene has recently been associated with plasma α-tocopherol concentration, suggesting a possible role of this protein in vitamin E intestinal absorption or tissue uptake.
  
  
  RESULTS
  To investigate the involvement of CD36 in vitamin E transport, we first evaluated the effect of CD36 on α- and γ-tocopherol transmembrane uptake and efflux using transfected HEK cells. γ-Tocopherol postprandial response was then assessed in CD36-deficient mice compared with wild-type mice, after the mice had been fully characterized for their α-tocopherol, vitamin A and lipid plasma, and tissue contents. Both α- and γ-tocopherol uptake was significantly increased in cells overexpressing CD36 compared with control cells. Compared with wild-type mice, CD36-deficient mice displayed a significantly decreased cholesterol hepatic concentration, and males exhibited significantly higher triacylglycerol contents in liver, brain, heart, and muscle. Although tissue α-tocopherol concentration after adjustment for lipid content was not modified, γ-tocopherol postprandial response was significantly increased in CD36-deficient mice compared with controls, likely reflecting the postprandial hypertriglyceridemia observed in these mice.
  
  
  CONCLUSIONS
  Our findings show for the first time that CD36 participates-directly or indirectly-in vitamin E uptake, and that CD36 effect on postprandial lipid metabolism in turn modifies vitamin E postprandial response.
authors.affiliation: INRA, UMR 1260, Nutrition, Obesity and Risk of Thrombosis, Marseille, France; INSERM, UMR 1062, Marseille, France; Aix-Marseille Université, Marseille, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Goncalves
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Roi
authors.forename: Marion
authors.initials: M
authors.lastname: Nowicki
authors.forename: Isabelle
authors.initials: I
authors.lastname: Niot
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Reboul
mesh_terms: D000818:Animals; D018955:CD36 Antigens / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D007408:Intestinal Absorption; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D020641:Polymorphism, Single Nucleotide; D019518:Postprandial Period / Q000187:drug effects; D014280:Triglycerides / Q000378:metabolism; D014801:Vitamin A / Q000097:blood / Q000493:pharmacokinetics; D024502:alpha-Tocopherol / Q000097:blood* / Q000493:pharmacokinetics; D024504:gamma-Tocopherol / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D018955:CD36 Antigens; D014280:Triglycerides; D014801:Vitamin A; D024504:gamma-Tocopherol; D002784:Cholesterol; D024502:alpha-Tocopherol
keywords: Bioavailability; CD36; Intestinal absorption; Transgenic mice; α-Tocopherol; γ-Tocopherol