title: Applications of low-flow LC-SRM for the analysis of large molecules in pharmaceutical R&D.
abstract: Although ligand-binding assays are frequently employed to measure large molecules, the use of LC-SRM assays is increasingly popular due to the inherent selectivity advantage and the ability to operate without exquisitely selective antibodies. Until recently LC-SRM assays have been unable to compete with ligand-binding assays in terms of sensitivity. However, the use of low-flow chromatography prior to mass spectrometry has played a crucial role in increasing the sensitivity of LC-SRM platforms and enabling measurements of large molecules that had previously been unmeasurable. In this article, we highlight some technical advances, describe strategies for employing low-flow chromatography, and review recent literature that describes implementation of low-flow LC-SRM to support large-molecule analysis in pharmaceutical R&D.
authors.affiliation: Merck & Co., 126 E Lincoln Ave, Rahway, NJ 07076, USA.
authors.forename: Michael E
authors.initials: ME
authors.lastname: Lassman
authors.forename: Carmen
authors.initials: C
authors.lastname: Fernandez-Metzler
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis / Q000302:isolation & purification / Q000493:pharmacokinetics; D015415:Biomarkers / Q000032:analysis*; D002853:Chromatography, Liquid; D004345:Drug Industry; D013058:Mass Spectrometry; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification; D012106:Research
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D010455:Peptides; D011506:Proteins