title: Lactate retards the development of erythrocytic stages of the human malaria parasite Plasmodium falciparum.
abstract: The intraerythrocytic form of the human malaria parasite Plasmodium falciparum relies on glycolysis for its energy requirements. In glycolysis, lactate is an end product. It is therefore known that lactate accumulates in in vitro culture; however, its influence on parasite growth remains unknown. Here we investigated the effect of lactate on the development of P. falciparum during in vitro culture under lactate supplementation in detail. Results revealed that lactate retarded parasite development and reduced the number of merozoites in the schizont stage. These findings suggest that lactate has the potential to affect parasite development.
authors.affiliation: Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan; Department of Microbiology and Immunology, Teikyo University School of Medicine, Itabashi, Tokyo, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Hikosaka
authors.affiliation: Department of Molecular and Cellular Parasitology, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hirai
authors.affiliation: Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Komatsuya
authors.affiliation: Department of Microbiology and Immunology, Teikyo University School of Medicine, Itabashi, Tokyo, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ono
authors.affiliation: Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan. Electronic address: kitak@m.u-tokyo.ac.jp.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Kita
mesh_terms: D004912:Erythrocytes / Q000469:parasitology*; D006019:Glycolysis; D006801:Humans; D019344:Lactic Acid / Q000494:pharmacology*; D052940:Merozoites / Q000187:drug effects / Q000254:growth & development; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000254:growth & development* / Q000648:ultrastructure; D052939:Schizonts / Q000187:drug effects / Q000254:growth & development
chemical_list: D019344:Lactic Acid
keywords: Developmental retardation; In vitro culture; Lactate; Malaria; Plasmodium