title: The GH26 β-mannanase RsMan26H from a symbiotic protist of the termite Reticulitermes speratus is an endo-processive mannobiohydrolase: heterologous expression and characterization.
abstract: Symbiotic protists in the gut of termites are prominent natural resources for enzymes involved in lignocellulose degradation. Here we report expression, purification, and biochemical characterization of a glycoside hydrolase family 26 mannanase RsMan26H from the symbiotic protist of the lower termite, Reticulitermes speratus. Biochemical analysis of RsMan26H demonstrates that this enzyme is an endo-processive mannobiohydrolase producing mannobiose from oligo- and polysaccharides, followed by a minor accumulation of oligosaccharides larger than mannobiose. To our knowledge, this is the first report describing the unique mannobiohydrolase enzyme from the eukaryotic origin.
authors.affiliation: Department of Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Tsukagoshi
authors.affiliation: Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Nakamura
authors.affiliation: Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Ishida
authors.affiliation: Biomass Engineering Program Cooperation Division, RIKEN, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Otagiri
authors.affiliation: Laboratory of Environmental Molecular Biology, Graduate School of Yokohama City University, Yokohama, Kanagawa 230-0045, Japan; Molecular and Informative Life Science Unit, RIKEN Advanced Science Institute, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Shigeharu
authors.initials: S
authors.lastname: Moriya
authors.affiliation: Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Samejima
authors.affiliation: Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Kiyohiko
authors.initials: K
authors.lastname: Igarashi
authors.affiliation: Department of Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Kitamoto
authors.affiliation: Department of Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan. Electronic address: arioka@mail.ecc.u-tokyo.ac.jp.
authors.forename: Manabu
authors.initials: M
authors.lastname: Arioka
mesh_terms: D000818:Animals; D015870:Gene Expression; D020049:Isoptera / Q000502:physiology; D007700:Kinetics; D008351:Mannans / Q000737:chemistry* / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D056900:Parabasalidea / Q000737:chemistry* / Q000201:enzymology; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D015800:Protozoan Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013559:Symbiosis; D044902:beta-Mannosidase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D008351:Mannans; D009844:Oligosaccharides; D011134:Polysaccharides; D015800:Protozoan Proteins; C014647:mannobiose; D044902:beta-Mannosidase
keywords: Glycoside hydrolase family 26; Mannanase; Pichia pastoris expression; Processivity; Symbiotic protist; Termite