title: Liver lobe and strain difference in gene expression after hydrodynamics-based gene delivery in mice.
abstract: Hydrodynamics-based gene delivery (HGD) is a widely recognized technique for delivering exogenous DNA with high efficiency to murine hepatocytes. In this study, we investigated stimulation of exogenous DNA uptake and expression using a commercially available reagent for HGD. We also examined which mouse strain and mouse liver lobe would achieve the best gene delivery performance. Mice were injected with a solution containing reporter plasmid DNA or DNA and a gene delivery reagent. One day after the HGD procedure, liver samples were isolated and subjected to biochemical and histochemical analyses. The reporter plasmid DNA showed the strongest signal when the DNA was dissolved in TransIT-EE Hydrodynamic Delivery Solution (Takara Bio Inc., Shiga, Japan). Evaluation of transgene expression in each hepatic lobe in ICR, C57BL/6N, Balb/cA, and B6C3F1 mice showed that ICR mice exhibited the best gene transfer and that the right median lobe had the highest level of transgene expression. These findings suggest the importance of choice in mouse strains and liver lobes when performing gene-based manipulations of the liver.
authors.affiliation: a Department of Surgery II , National Defense Medical College , Saitama , Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Nakamura
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Maehara
authors.forename: Satoshi
authors.initials: S
authors.lastname: Watanabe
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishihara
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sato
mesh_terms: D000818:Animals; D015870:Gene Expression; D018014:Gene Transfer Techniques; D008099:Liver / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D013045:Species Specificity
keywords: CAG, cytomegalovirus enhancer + chicken β-actin promoter; EGFP, enhanced green fluorescent protein; HGD, hydrodynamics-based gene delivery; Hydrodynamics-based gene delivery (HGD); IRES, internal ribosomal entry site; In vivo gene transfer; Liver lobe; Nonviral vector; PEI, polyethylenimine; RLU, relative light units; SVp, SV40 early promoter; Strain difference; luc, luciferase; pA, poly(A) sites