title: 1/fα noise in the dynamic force spectroscopy curves signals the occurrence of biorecognition.
abstract: Biorecognition leads to the formation of a specific complex between a couple of biological partners to accomplish a functional task. Its occurrence can be inferred a posteriori by analyzing the unbinding force curves in a dynamic force spectroscopy experiment. Because of nonspecific interactions, the method is not, however, exempt from ambiguities and subjectivity. A fingerprint of the partner recruitment in the complex has been disclosed in the fluctuations of the atomic force microscopy cantilever. We demonstrate that the formation of the biotin-avidin specific complex strongly correlates with a 1/f(α) noise in the force curve fluctuations.
authors.affiliation: Biophysics and Nanoscience Centre, CNISM-DEB, Universita' della Tuscia, 01100 Viterbo, Italy.
authors.forename: Anna Rita
authors.initials: AR
authors.lastname: Bizzarri
authors.affiliation: Biophysics and Nanoscience Centre, CNISM-DEB, Universita' della Tuscia, 01100 Viterbo, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Cannistraro
mesh_terms: D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D008954:Models, Biological; D013057:Spectrum Analysis / Q000379:methods*; D019809:Streptavidin / Q000378:metabolism
chemical_list: D001360:Avidin; D001710:Biotin; D019809:Streptavidin