title: Carob pod water extracts as feedstock for succinic acid production by Actinobacillus succinogenes 130Z.
abstract: Carob pods are a by-product of locust bean gum industry containing more than 50% (w/w) sucrose, glucose and fructose. In this work, carob pod water extracts were used, for the first time, for succinic acid production by Actinobacillus succinogenes 130Z. Kinetic studies of glucose, fructose and sucrose consumption as individual carbon sources till 30g/L showed no inhibition on cell growth, sugar consumption and SA production rates. Sugar extraction from carob pods was optimized varying solid/liquid ratio and extraction time, maximizing sugar recovery while minimizing the extraction of polyphenols. Batch fermentations containing 10-15g/L total sugars resulted in a maximum specific SA production rate of 0.61Cmol/Cmol X.h, with a yield of 0.55Cmol SA/Cmol sugar and a volumetric productivity of 1.61g SA/L.h. Results demonstrate that carob pods can be a promising low cost feedstock for bio-based SA production.
authors.affiliation: REQUIMTE, DQ/FCT, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Margarida
authors.initials: M
authors.lastname: Carvalho
authors.affiliation: REQUIMTE, DQ/FCT, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal. Electronic address: christophe.roca@fct.unl.pt.
authors.forename: Christophe
authors.initials: C
authors.lastname: Roca
authors.affiliation: REQUIMTE, DQ/FCT, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Maria A M
authors.initials: MAM
authors.lastname: Reis
mesh_terms: D000188:Actinobacillus / Q000378:metabolism*; D000704:Analysis of Variance; D019149:Bioreactors; D053898:Biosynthetic Pathways / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D007887:Fabaceae / Q000737:chemistry*; D005285:Fermentation; D005632:Fructose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D010936:Plant Extracts / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D019802:Succinic Acid / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism; D014867:Water
chemical_list: D010936:Plant Extracts; D014867:Water; D005632:Fructose; D013395:Sucrose; D019802:Succinic Acid; D005947:Glucose
keywords: Actinobacillus succinogenes; Carob pod; Fermentation; Succinic acid