title: Characterization of CTL by microscopy.
abstract: Conventional microscopic techniques may be easily employed to detect and characterize cytotoxic T lymphocytes (CTL). Immunohistochemistry (IHC) may be effectively performed in CTL characterization to identify the location and distribution of target antigens in suspension cells or in tissues by staining with a specific antibody. The antibody may be conjugated to either a fluorescent or an enzymatic label, and the location of the label seen through a microscope approximates the position of the target antigen. Here, immunohistochemistry and immunofluorescence for both paraffin-embedded and frozen tissues and for suspended cell are described. Moreover, specific recommendations for analysis of suspension of cells are provided.
authors.affiliation: Department of Medical and Surgical Sciences, University of Foggia, Ospedali Riuniti, Viale Luigi Pinto, 1, 71122, Foggia, Italy, giuseppestefano.netti@unifg.it.
authors.forename: Giuseppe Stefano
authors.initials: GS
authors.lastname: Netti
mesh_terms: D002460:Cell Line; D015925:Cryopreservation; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D016612:Paraffin Embedding; D013194:Staining and Labeling; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology*; D016707:Tissue Fixation / Q000379:methods