title: Proteomic analysis of Fusarium graminearum treated by the fungicide JS399-19.
abstract: JS399-19 (2-cyano-3-amino-3-phenylancryic acetate), a novel cyanoacrylate fungicide, has powerful inhibition against Fusarium species, especially to Fusarium graminearum. Treated with JS399-19, mycelium of F. graminearum was distorted and swelled. The embranchment increased. In order to investigate the effect of JS399-19 on protein expression of F. graminearum, total protein of F. graminearum cultured in normal condition and that treated with 0.5 μg/mL (EC90 value) JS399-19 were extracted respectively and proteomic analysis was performed using two-dimensional gel electrophoresis. The expression levels of 38 proteins varied quantitatively at least twofold. 33 proteins out of the 38 were successfully identified by MALDI-TOF-MS/MS and MASCOT. According to the classification of physiological functions from Conserved Domain Database analysis, 19, 5, 2, 3, 2 and 2 proteins were respectively associated with metabolism, regulation, motility, defense, signal transduction, and unknown function, which indicated that energy metabolism, the synthesis and transport of proteins and DNA of F. graminearum were inhibited by JS399-19 in different degrees. The expression levels of the genes were further confirmed by quantitative real-time PCR analyses. This study represents the first proteomic analysis of F. graminearum treated by JS399-19 and will provide some useful information to find the mode of action of the fungicide against F. graminearum.
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Education, Nanjing 210095, China; Key Laboratory of Pesticide, Jiangsu Province, Nanjing 210095, China. Electronic address: houyiping@njau.edu.cn.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Hou
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Education, Nanjing 210095, China; Key Laboratory of Pesticide, Jiangsu Province, Nanjing 210095, China.
authors.forename: Zhitian
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Education, Nanjing 210095, China; Key Laboratory of Pesticide, Jiangsu Province, Nanjing 210095, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Xu
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Education, Nanjing 210095, China; Key Laboratory of Pesticide, Jiangsu Province, Nanjing 210095, China.
authors.forename: Changjun
authors.initials: C
authors.lastname: Chen
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Education, Nanjing 210095, China; Key Laboratory of Pesticide, Jiangsu Province, Nanjing 210095, China. Electronic address: mgzhou@njau.edu.cn.
authors.forename: Mingguo
authors.initials: M
authors.lastname: Zhou
mesh_terms: D000596:Amino Acids / Q000494:pharmacology*; D004271:DNA, Fungal / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects; D005656:Fungal Proteins / Q000378:metabolism*; D005659:Fungicides, Industrial / Q000494:pharmacology*; D005670:Fusarium / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D010666:Phenylpropionates / Q000494:pharmacology*; D021381:Protein Transport / Q000187:drug effects; D040901:Proteomics; D012331:RNA, Fungal / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects
chemical_list: C540078:2-cyano-3-amino-3-phenylacrylic acid; D000596:Amino Acids; D004271:DNA, Fungal; D005656:Fungal Proteins; D005659:Fungicides, Industrial; D010666:Phenylpropionates; D012331:RNA, Fungal
keywords: Fusarium graminearum; JS399-19; Mycelial growth; Protein expression