title: A web-based tool for rational screening of mutants libraries using ProSAR.
abstract: In directed evolution experiments, it is at stake to have methods to screen efficiently the mutant libraries. We propose a web-based tool that implements an established in silico method for the rational screening of mutant libraries. The method, known as ProSAR, attempts to link sequence data to activity. The method uses statistical models trained on small experimental datasets provided by the user. These can integrate potential epistatic interactions between mutations and be used in many diverse biological contexts. It drastically improves the search for leading mutants. The tool is freely available to non-commercial users at http://bo-protscience.fr/prosar/.
authors.affiliation: DSIMB, INSERM UMR-S1134, Université de La Réunion, 97715 Saint-Denis, France Laboratory of Excellence GR-Ex, 75015 Paris, France Université de Nantes, UFIP, UMR CNRS 6286, 2 chemin de la Houssinière, 44000 Nantes, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Berland
authors.affiliation: Université de Nantes, UFIP, UMR CNRS 6286, 2 chemin de la Houssinière, 44000 Nantes, France Peaccel SAS, Saint-Denis, La Réunion, France bernard.offmann@univ-nantes.fr.
authors.forename: Bernard
authors.initials: B
authors.lastname: Offmann
authors.affiliation: Université de Toulouse; INSA, UPS, INP; LISBP, 135 Avenue de Rangueil, 31077 Toulouse, France CNRS, UMR 5504, 31400 Toulouse, France INRA, UMR 792 Ingénierie des Systèmes Biologiques et des Procédés, 31400 Toulouse, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: André
authors.affiliation: Université de Toulouse; INSA, UPS, INP; LISBP, 135 Avenue de Rangueil, 31077 Toulouse, France CNRS, UMR 5504, 31400 Toulouse, France INRA, UMR 792 Ingénierie des Systèmes Biologiques et des Procédés, 31400 Toulouse, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Remaud-Siméon
authors.affiliation: DSIMB, INSERM UMR-S1134, Université de La Réunion, 97715 Saint-Denis, France Laboratory of Excellence GR-Ex, 75015 Paris, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Charton
mesh_terms: D000465:Algorithms; D030562:Databases, Protein; D019020:Directed Molecular Evolution; D015723:Gene Library; D020407:Internet; D009154:Mutation; D040901:Proteomics / Q000379:methods*; D012044:Regression Analysis; D012984:Software
keywords: epistatic interactions between mutations; fitness landscape exploration; protein engineering; rational screening; sequence–activity relationship