title: Activation of intrinsic immune responses and microglial phagocytosis in an ex vivo spinal cord slice culture model of West Nile virus infection.
abstract: West Nile virus (WNV) is a neurotropic flavivirus that causes significant neuroinvasive disease involving the brain and/or spinal cord. Experimental mouse models of WNV infection have established the importance of innate and adaptive immune responses in controlling the extent and severity of central nervous system (CNS) disease. However, differentiating between immune responses that are intrinsic to the CNS and those that are dependent on infiltrating inflammatory cells has proven difficult. We used a murine ex vivo spinal cord slice culture (SCSC) model to determine the innate immune processes specific to the CNS during WNV infections. By 7 days after ex vivo infection of SCSCs, the majority of neurons and a substantial percentage of astrocytes were infected with WNV, resulting in apoptotic cell death and astrogliosis. Microglia, the resident immune cells of the CNS, were activated by WNV infection, as exemplified by their amoeboid morphology, the development of filopodia and lamellipodia, and phagocytosis of WNV-infected cells and debris. Microglial cell activation was concomitant with increased expression of proinflammatory cytokines and chemokines, including CXCL10, CXCL1, CCL5, CCL3, CCL2, tumor necrosis factor alpha (TNF-α), TNF-related apoptosis-inducing ligand (TRAIL), and interleukin-6 (IL-6). The application of minocycline, an inhibitor of neuroinflammation, altered the WNV-induced proinflammatory cytokine/chemokine expression profile, with inhibited production of CCL5, CCL2, and IL-6. Our findings establish that CNS-resident cells have the capacity to initiate a robust innate immune response against WNV infection in the absence of infiltrating inflammatory cells and systemic immune responses.
  
  
  OBJECTIVE
  There are no specific treatments of proven efficacy available for WNV neuroinvasive disease. A better understanding of the pathogenesis of WNV CNS infection is crucial for the rational development of novel therapies. Development of a spinal cord slice culture (SCSC) model facilitates the study of WNV pathogenesis and allows investigation of the intrinsic immune responses of the CNS. Our studies demonstrate that robust CNS innate immune responses, including microglial activation and proinflammatory cytokine/chemokine production, develop independently of contributions from the peripheral immune system and CNS-infiltrating inflammatory cells.
authors.affiliation: Neuroscience Program, University of Colorado Denver, Aurora, Colorado, USA.
authors.forename: Eamon D
authors.initials: ED
authors.lastname: Quick
authors.affiliation: Department of Neurology, University of Colorado Denver, Aurora, Colorado, USA.
authors.forename: J Smith
authors.initials: JS
authors.lastname: Leser
authors.affiliation: Department of Neurology, University of Colorado Denver, Aurora, Colorado, USA Penny.Clarke@ucdenver.edu.
authors.forename: Penny
authors.initials: P
authors.lastname: Clarke
authors.affiliation: Neuroscience Program, University of Colorado Denver, Aurora, Colorado, USA Department of Neurology, University of Colorado Denver, Aurora, Colorado, USA Department of Immunology and Microbiology, University of Colorado Denver, Aurora, Colorado, USA Department of Infectious Disease, University of Colorado Denver, Aurora, Colorado, USA Department of Medicine, University of Colorado Denver, Aurora, Colorado, USA Denver VA Medical Center, Denver, Colorado, USA.
authors.forename: Kenneth L
authors.initials: KL
authors.lastname: Tyler
mesh_terms: D000818:Animals; D017209:Apoptosis; D001253:Astrocytes / Q000502:physiology / Q000821:virology; D051379:Mice; D017628:Microglia / Q000276:immunology*; D009474:Neurons / Q000502:physiology / Q000821:virology; D009924:Organ Culture Techniques; D010587:Phagocytosis; D013116:Spinal Cord / Q000276:immunology* / Q000821:virology*; D014776:Virus Cultivation; D014902:West Nile virus / Q000254:growth & development* / Q000276:immunology*