title: Roseivivax jejudonensis sp. nov., isolated from the junction between the ocean and a freshwater spring at Jeju Island, South Korea.
abstract: A Gram-stain negative, coccoid- or oval-shaped and non-gliding bacterial strain, designated CDM-17(T), was isolated from the zone where the ocean and a freshwater spring meet at Jeju island, South Korea, and subjected to a polyphasic taxonomic study. Strain CDM-17(T) was observed to form smooth, circular, glistening, slightly convex, light yellowish pink colonies on marine agar, and was found to grow optimally at pH 7.0-8.0, at 30 °C and in the presence of 2-3 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain CDM-17(T) fell within the clade comprising the Roseivivax species, clustering with the type strain of Roseivivax sediminis with which it exhibited 98.3 % sequence similarity value. Sequence similarities to the type strains of the other recognized Roseivivax species were 94.7-96.8 %. Strain CDM-17(T) was found to contain Q-10 as the predominant ubiquinone and summed feature 8 (C18:1 ω6c and/or C18:1 ω7c) as the major fatty acid. The major polar lipids were identified as phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid, an unidentified glycolipid and an unidentified lipid. The DNA G+C content of strain CDM-17(T) was determined to be 66.2 mol% and its mean DNA-DNA relatedness value with Rsv. sediminis KCTC 23444(T) was 17.5 ± 2.7 %. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain CDM-17(T) is distinguishable from recognized Roseivivax species. On the basis of the data presented, strain CDM-17(T) is proposed to represent a novel species of the genus Roseivivax, for which the name Roseivivax jejudonensis sp. nov. is proposed. The type strain is CDM-17(T) (=KCTC 42110(T) = CECT 8625(T)).
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Lee
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006017:Glycolipids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride