title: Investigation of association between donors' and recipients' NADPH oxidase p22(phox) C242T polymorphism and acute rejection, delayed graft function and blood pressure in renal allograft recipients.
abstract: BACKGROUND
  Production of reactive oxygen species (ROS) and thereby induction of oxidative stress seem to be one of the major mediators of inflammatory adverse outcomes after renal transplantation. p22(phox) is a polymorphic subunit of NAD(P)H-oxidase that is critical for activation and stabilization of the enzyme. This enzyme is involved in the production of superoxide that triggers inflammatory injuries to the kidney. So in this study, the association between donors and recipients' C242T polymorphism of p22(phox) and acute rejection (AR), delayed graft function (DGF), creatinine clearance (CrCl), and blood pressure in renal-allograft recipients was studied.
  
  
  METHODS
  One hundred ninety six donor-recipient pairs were studied. The C242T polymorphism of p22(phox) was determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). According to p22 genotype, the subjects were divided in wild-type (CC) and T allele carriers (CT+TT). Transplantation outcomes were determined using acute rejection and delayed graft function criteria. The mean arterial pressure was also measured monthly after transplantation.
  
  
  RESULTS
  There was a significant association between the recipients' p22(phox) polymorphism and DGF occurrence (OR=2.5, CI: 1.2-4.9, p=0.0009). No significant association was detected between donors' p22(phox) polymorphism and AR and DGF events. CrCl during the six months follow-up after transplantation was lower in the patients who received allograft from donors carrying 242T allele (B=-12.8, CI: -22.9-12.8 (-22.9 to -2.6)). Changes in the blood pressure were not different among the patients having different genotypes of p22(phox).
  
  
  CONCLUSIONS
  These results suggest that the recipients' p22(phox) C242T polymorphism may be a major risk factor for DGF in renal transplantation. Moreover, the donors' 242T allele seems to affect the rate of CrCl in the renal allograft recipients.
authors.affiliation: Pharmaceutics Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, Iran; Department of Pharmacology & Toxicology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran; Physiology Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Mandegary
authors.affiliation: Department of Nephrology, Urology and Renal Transplantation, Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Rahmanian-Koshkaki
authors.affiliation: Department of Nephrology, Urology and Renal Transplantation, Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Mohammad-Amir
authors.initials: MA
authors.lastname: Mohammadifar
authors.affiliation: Department of Pharmacology & Toxicology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran; Department of Molecular Pathology, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Pourgholi
authors.affiliation: Department of Pharmacology & Toxicology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Mehdipour
authors.affiliation: Department of Nephrology, Urology and Renal Transplantation, Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Etminan
authors.affiliation: Department of Nephrology, Urology and Renal Transplantation, Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Mohammad-Reza
authors.initials: MR
authors.lastname: Ebadzadeh
authors.affiliation: Department of Nephrology, Urology and Renal Transplantation, Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Faramarz
authors.initials: F
authors.lastname: Fazeli
authors.affiliation: Physiology Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, Iran; Department of Nephrology, Urology and Renal Transplantation, Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, Iran. Electronic address: J_azmandian@yahoo.com.
authors.forename: Jalal
authors.initials: J
authors.lastname: Azmandian
mesh_terms: D000208:Acute Disease; D000328:Adult; D000483:Alleles; D005260:Female; D005500:Follow-Up Studies; D006084:Graft Rejection / Q000201:enzymology / Q000235:genetics* / Q000276:immunology; D006801:Humans; D016030:Kidney Transplantation; D019520:Living Donors; D008297:Male; D019255:NADPH Oxidases / Q000235:genetics* / Q000276:immunology; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D019255:NADPH Oxidases; C086986:CYBA protein, human
keywords: Blood pressure; Delayed graft function; Graft rejection; NADH oxidase; Renal transplantation; p22(phox) C242T polymorphism