title: Anthelmintic, antimicrobial, antioxidant and cytotoxic activity of Caltha palustris var. alba Kashmir, India.
abstract: The methanolic extract obtained from the root portion of Caltha palustris var. alba was evaluated for its anthelmintic efficacy against gastrointestinal nematodes of sheep under both in vitro and in vivo conditions using worm motility inhibition (WMI) assay and fecal egg count reduction (FECR) assay, respectively. The extract was subjected to antimicrobial activity using agar-well diffusion method against different bacterial strains. In addition the extract was evaluated for cytotoxic and antioxidant activity against cultured THP-1(Leukemia), A-549 (Lung), HCT-15 (Colon), Cervix (HeLa) and PC-3(Prostrate) cell lines by SRB and DPPH radical scavenging assays. The extract used resulted in mean %WMI of 94.44%, as observed when the worms were put in lukewarm buffer for 30 min after exposure to different treatments. The mean mortality index of the sample was 0.95. The lethal concentration (LC50) was 0.11 mg·mL(-1). Cell lines were exposed to concentration of 100 μg·mL(-1) of extract for 48 h, which reduced the viability of these cell lines. The same plant extract also showed 55.58% DPPH radical scavenging activity.
authors.affiliation: Department of Chemistry, University of Kashmir, Hazratbal Srinagar-190006, Jammu and Kashmir, India.
authors.forename: Sofi
authors.initials: S
authors.lastname: Mubashir
authors.affiliation: Department of Chemistry, University of Kashmir, Hazratbal Srinagar-190006, Jammu and Kashmir, India.
authors.forename: Mohd Yousuf
authors.initials: MY
authors.lastname: Dar
authors.affiliation: Centre of Research for Development, University of Kashmir, Hazratbal Srinagar, Jammu and Kashmir, India.
authors.forename: Bashir A
authors.initials: BA
authors.lastname: Lone
authors.affiliation: Department of Pharmaceutical Sciences, University of Kashmir, Hazratbal Srinagar, 190006, Jammu and Kashmir, India.
authors.forename: M Iqbal
authors.initials: MI
authors.lastname: Zargar
authors.affiliation: Department of Chemistry, University of Kashmir, Hazratbal Srinagar-190006, Jammu and Kashmir, India. Electronic address: doctorwajaht@gmail.com.
authors.forename: Wajaht A
authors.initials: WA
authors.lastname: Shah
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D001713:Biphenyl Compounds / Q000378:metabolism; D005243:Feces / Q000469:parasitology; D041981:Gastrointestinal Tract / Q000469:parasitology; D006367:HeLa Cells; D006801:Humans; D009348:Nematoda / Q000187:drug effects*; D009369:Neoplasms / Q000188:drug therapy; D008517:Phytotherapy; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D029626:Ranunculaceae; D012756:Sheep / Q000469:parasitology
chemical_list: D000871:Anthelmintics; D000900:Anti-Bacterial Agents; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Anthelmintic activity (in vivo and in vitro); Antimicrobial activity; Caltha palustris var. alba; Cytotoxic activity; Radical scavenging activity