title: Mineralized gelatin methacrylate-based matrices induce osteogenic differentiation of human induced pluripotent stem cells.
abstract: Human induced pluripotent stem cells (hiPSC) are a promising cell source with pluripotency and self-renewal properties. Design of simple and robust biomaterials with an innate ability to induce lineage-specificity of hiPSC is desirable to realize their application in regenerative medicine. In this study, the potential of biomaterials containing calcium phosphate minerals to induce osteogenic differentiation of hiPSC was investigated. hiPSC cultured using mineralized gelatin methacrylate-based matrices underwent osteogenic differentiation ex vivo, in both two-dimensional and three-dimensional cultures, in growth medium devoid of any osteogenic-inducing chemical components or growth factors. The findings that osteogenic differentiation of hiPSC can be achieved through biomaterial-based cues alone present new avenues for personalized regenerative medicine. Such biomaterials that could not only act as structural scaffolds, but could also provide tissue-specific functions such as directing stem cell differentiation commitment, have great potential in bone tissue engineering.
authors.affiliation: Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA; Materials Science and Engineering Program, University of California, San Diego, La Jolla, CA 92093, USA.
authors.forename: Heemin
authors.initials: H
authors.lastname: Kang
authors.affiliation: Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA.
authors.forename: Yu-Ru V
authors.initials: YV
authors.lastname: Shih
authors.affiliation: Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA.
authors.forename: Yongsung
authors.initials: Y
authors.lastname: Hwang
authors.affiliation: Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA; School of Chemistry and Chemical Engineering, Southeast University, Nanjing 210018, China.
authors.forename: Cai
authors.initials: C
authors.lastname: Wen
authors.affiliation: Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA.
authors.forename: Vikram
authors.initials: V
authors.lastname: Rao
authors.affiliation: Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA; Department of Nanoengineering, University of California, San Diego, La Jolla, CA 92093, USA.
authors.forename: Timothy
authors.initials: T
authors.lastname: Seo
authors.affiliation: Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA; Materials Science and Engineering Program, University of California, San Diego, La Jolla, CA 92093, USA; Department of Nanoengineering, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: svarghese@ucsd.edu.
authors.forename: Shyni
authors.initials: S
authors.lastname: Varghese
mesh_terms: D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000494:pharmacology*; D018786:Bone Substitutes / Q000138:chemical synthesis / Q000494:pharmacology*; D002113:Calcification, Physiologic / Q000502:physiology; D002130:Calcium Phosphates / Q000737:chemistry; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D008689:Methacrylates; D005780:Gelatin; C020243:calcium phosphate
keywords: Bone tissue engineering; Calcium phosphate; Gelatin methacrylate; Human induced pluripotent stem cells; Osteogenic differentiation