title: CspA encodes a major cold shock protein in Himalayan psychrotolerant Pseudomonas strains.
abstract: The major cold-shock protein (CspA) encoding gene cspA were detected in three Himalayan psychrotrophic Pseudomonad strains, by PCR amplification. Partial sequencing of three Pseudomonas strains cspA gene and BLAST search confirmed the high similarity with putative bacterial cspA gene and bacterial CspA protein. Bioinformatics analysis of these partial CspA amino acid sequences showed presence of putative conserved region for DNA/RNA-binding motifs RNP-1 and RNP-2. Protein homologies of all three bacterial CspA proteins belong to S1 like protein (Ribosomal protein S1-like RNA-binding domain). Presence of cspA gene and its high similarity with Bacillus cereus group demonstrating uniqueness of cspA gene in these Pseudomonas strains and suggesting strong evolutionary relationship between these two groups to survive in cold environments. Probable CspA protein expression levels were checked after cold shock (28°C to 4°C) and cold acclimation (4°C and 15°C) experiment. SDS-PAGE analysis revealed a small protein of approximate size of 7.5 kDa was expressed after cold shock (28°C to 4°C) and continuously over-expressed with the incubation time at cold temperature (4°C). Therefore it was predicted this protein would be product of cspA gene and suggesting this protein aids survival in Himalayan environments.
authors.affiliation: Department of Biotechnology, H.N.B Garhwal University, Srinagar, 246174, Uttarakhand, India, shekharcbisht@gmail.com.
authors.forename: Shekhar Chandra
authors.initials: SC
authors.lastname: Bisht
authors.forename: Gopal Kishna
authors.initials: GK
authors.lastname: Joshi
authors.forename: Pankaj Kumar
authors.initials: PK
authors.lastname: Mishra
mesh_terms: D000064:Acclimatization / Q000235:genetics*; D000531:Altitude; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis*; D001407:Bacillus / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D005075:Biological Evolution; D057773:Cold Shock Proteins and Peptides / Q000235:genetics* / Q000378:metabolism; D003080:Cold Temperature; D017124:Conserved Sequence; D017753:Ecosystem; D008969:Molecular Sequence Data; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics; D017385:Sequence Homology; D013312:Stress, Physiological / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D057773:Cold Shock Proteins and Peptides; D012269:Ribosomal Proteins; C062260:cold shock protein CS7.4, Bacteria; C015494:ribosomal protein S1