title: Induction of passive EAE using myelin-reactive CD4+ T cells.
abstract: Experimental autoimmune encephalomyelitis (EAE) is an autoimmune disease of the central nervous system (CNS) often used as a model for the early inflammatory stages of multiple sclerosis and also as a model of organ-specific autoimmune disease.This protocol describes the induction of passive EAE in mice, either using T cells isolated from mice primed with myelin antigens, or through the use of naïve TCR transgenic T cells activated in vitro in the presence of myelin-derived antigens.
authors.affiliation: MRC Centre for Inflammation Research and Centre for Multiple Sclerosis Research, Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.
authors.forename: Rhoanne C
authors.initials: RC
authors.lastname: McPherson
authors.forename: Helen E
authors.initials: HE
authors.lastname: Cambrook
authors.forename: Richard A
authors.initials: RA
authors.lastname: O'Connor
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Anderton
mesh_terms: D000276:Adjuvants, Immunologic; D019264:Adoptive Transfer; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000637:transplantation*; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D018014:Gene Transfer Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009169:Mycobacterium tuberculosis / Q000276:immunology; D009186:Myelin Sheath / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D018417:Th1 Cells / Q000378:metabolism / Q000637:transplantation
chemical_list: D000276:Adjuvants, Immunologic; D011948:Receptors, Antigen, T-Cell