title: Group I mGluR-dependent depotentiation in the lateral amygdala does not require the removal of calcium-permeable AMPA receptors.
abstract: There is conflicting evidence regarding whether calcium-permeable receptors are removed during group I mGluR-mediated synaptic depression. In support of this hypothesis, AMPAR rectification, a correlative index of the synaptic expression of GluA2-lacking calcium-permeable AMPARs (CP-AMPARs), is known to decrease after the induction of several types of group I mGluR-mediated long-term depression (LTD), suggesting that a significant proportion of synaptic CP-AMPARs is removed during synaptic depression. We have previously demonstrated that fear conditioning-induced synaptic potentiation in the lateral amygdala is reversed by group 1 mGluR-mediated depotentiation. Here, we examined whether CP-AMPARs are removed by mGluR1-mediated depotentiation of fear conditioning-induced synaptic potentiation. The synaptic expression of CP-AMPARs was negligible before, increased significantly 12 h after, and returned to baseline 48 h after fear conditioning, as evidenced by the changes in the sensitivity of lateral amygdala synaptic responses to NASPM. Importantly, the sensitivity to NASPM was not altered after induction of depotentiation. Our findings, together with previous results, suggest that the removal of CP-AMPARs is not required for the depotentiation of fear conditioning-induced synaptic potentiation at lateral amygdala synapses.
authors.affiliation: School of Biological Sciences, College of Natural Sciences, Seoul National University Seoul, Korea (ROK).
authors.forename: Kyungjoon
authors.initials: K
authors.lastname: Park
authors.affiliation: School of Biological Sciences, College of Natural Sciences, Seoul National University Seoul, Korea (ROK).
authors.forename: Sukwoon
authors.initials: S
authors.lastname: Song
authors.affiliation: The Solomon H. Snyder Department of Neuroscience, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine Baltimore, MD, USA.
authors.forename: Ingie
authors.initials: I
authors.lastname: Hong
authors.affiliation: Institute of Neuroscience, Technical University of Munich Munich, Germany.
authors.forename: Beomjong
authors.initials: B
authors.lastname: Song
authors.affiliation: Center for Neural Science and Center for Connectomics, Korea Institute of Science and Technology Seoul, Korea (ROK).
authors.forename: Jeongyeon
authors.initials: J
authors.lastname: Kim
authors.affiliation: School of Biological Sciences, College of Natural Sciences, Seoul National University Seoul, Korea (ROK).
authors.forename: Sungmo
authors.initials: S
authors.lastname: Park
authors.affiliation: School of Biological Sciences, College of Natural Sciences, Seoul National University Seoul, Korea (ROK).
authors.forename: Junuk
authors.initials: J
authors.lastname: Lee
authors.affiliation: Center for Neural Science and Center for Connectomics, Korea Institute of Science and Technology Seoul, Korea (ROK).
authors.forename: Sangho
authors.initials: S
authors.lastname: Song
authors.affiliation: School of Biological Sciences, College of Natural Sciences, Seoul National University Seoul, Korea (ROK).
authors.forename: Bobae
authors.initials: B
authors.lastname: An
authors.affiliation: School of Biological Sciences, College of Natural Sciences, Seoul National University Seoul, Korea (ROK).
authors.forename: Jihye
authors.initials: J
authors.lastname: Kim
authors.affiliation: Center for Neural Science and Center for Connectomics, Korea Institute of Science and Technology Seoul, Korea (ROK).
authors.forename: C Justin
authors.initials: CJ
authors.lastname: Lee
authors.affiliation: Department of Biology, Department of Life and Nanopharmaceutical Sciences, Kyung Hee University Seoul, Korea (ROK).
authors.forename: Ki Soon
authors.initials: KS
authors.lastname: Shin
authors.affiliation: School of Biological Sciences, College of Natural Sciences, Seoul National University Seoul, Korea (ROK).
authors.forename: Sukwoo
authors.initials: S
authors.lastname: Choi
authors.affiliation: School of Biological Sciences, College of Natural Sciences, Seoul National University Seoul, Korea (ROK).
authors.forename: Sukwon
authors.initials: S
authors.lastname: Lee
keywords: calcium-permeable AMPA receptors; fear conditioning; lateral amygdala; long-term depression; synaptic depotentiation