title: Preferential localization of type I phosphatidylinositol 4-phosphate 5-kinase γ at the periactive zone of mouse photoreceptor ribbon synapses.
abstract: Type I phosphatidylinositol 4-phosphate 5 kinase γ (PIP5KIγ) constitutes a major pathway for the generation of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) that regulates a variety of neuronal functions at both presynaptic and postsynaptic compartments. In this study, we examined the expression and localization of PIP5KIγ in the adult mouse retina. RT-PCR analysis revealed that PIP5KIγ_v2 was predominantly expressed in the retina while PIP5KIγ_v3 was also expressed faintly. Immunostaining of the adult mouse retina revealed intense PIP5KIγ-immunoreactivity in the inner and outer plexiform layers in a punctate manner. In the photoreceptor ribbon synapse, PIP5KIγ was highly concentrated at the periactive zone. These findings suggest that PIP5KIγ, especially PIP5KIγ_i2, is localized at the periactive zone, a functionally suitable compartment for the endocytosis of synaptic vesicles in photoreceptor ribbon synapses.
authors.affiliation: Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Kanagawa 252-0374, Japan. Electronic address: sakagami@med.kitasato-u.ac.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sakagami
authors.affiliation: Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Katsumata
authors.affiliation: Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Hara
authors.affiliation: Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Tamaki
authors.affiliation: Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Fukaya
mesh_terms: D000429:Alcohol Oxidoreductases; D000818:Animals; D064026:Calbindins / Q000378:metabolism; D002966:Clathrin / Q000378:metabolism; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins / Q000378:metabolism; D000076122:Disks Large Homolog 4 Protein; D051528:Guanylate Kinases / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D016253:Microscopy, Immunoelectron; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D010786:Photoreceptor Cells / Q000378:metabolism* / Q000648:ultrastructure; D011493:Protein Kinase C / Q000378:metabolism; D012160:Retina / Q000166:cytology; D013569:Synapses / Q000378:metabolism* / Q000648:ultrastructure; D016608:Talin / Q000378:metabolism; D050495:Vesicular Inhibitory Amino Acid Transport Proteins / Q000378:metabolism
chemical_list: C498441:Bsn protein, mouse; D064026:Calbindins; D002966:Clathrin; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins; D000076122:Disks Large Homolog 4 Protein; C501447:Dlg4 protein, mouse; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D016608:Talin; D050495:Vesicular Inhibitory Amino Acid Transport Proteins; C492244:Viaat protein, mouse; D000429:Alcohol Oxidoreductases; C111341:Ctbp2 protein, mouse; D017853:Phosphotransferases (Alcohol Group Acceptor); C020077:1-phosphatidylinositol-4-phosphate 5-kinase; D011493:Protein Kinase C; D051528:Guanylate Kinases
keywords: Active zone; Endocytosis; Phosphoinositide; Photoreceptor; Retina; Ribbon synapse