title: Molecular characterization of DDT resistance in Anopheles gambiae from Benin.
abstract: BACKGROUND
  Insecticide resistance in the mosquito vector is the one of the main obstacles against effective malaria control. In order to implement insecticide resistance management strategies, it is important to understand the genetic factors involved. In this context, we investigated the molecular basis of DDT resistance in the main malaria vector from Benin.
  
  
  METHODS
  Anopheles gambiae mosquitoes were collected from four sites across Benin and identified to species/molecular form. Mosquitoes from Cotonou (M-form), Tori-Bossito (S-form) and Bohicon (S-form) were exposed to DDT 4% at a range of exposure times (30 min to 300 min). Another batch of mosquitoes from Cotonou and Malanville were exposed to DDT for 1 hour and the survivors 48 hours post exposure were used to quantify metabolic gene expression. Quantitative PCR assays were used to quantify mRNA levels of metabolic enzymes: GSTE2, GSTD3, CYP6P3 and CYP6M2. Expression (fold-change) was calculated using the ∆∆Ct method and compared to susceptible strains. Detection of target-site mutations (L1014F, L1014S and N1575Y) was performed using allelic discrimination TaqMan assays.
  
  
  RESULTS
  DDT resistance was extremely high in all populations, regardless of molecular form, with no observed mortality after 300 min exposure. In both DDT-survivors and non-exposed mosquitoes, GSTE2 and GSTD3 were over-expressed in the M form at 4.4-fold and 3.5-fold in Cotonou and 1.5-fold and 2.5-fold in Malanville respectively, when compared to the susceptible strain. The CYP6M2 and CYP6P3 were over-expressed at 4.6-fold and 3.8-fold in Cotonou and 1.2-fold and 2.5-fold in Malanville respectively. In contrast, no differences in GSTE2 and CYP6M2 were observed between S form mosquitoes from Tori-Bossito and Bohicon compared to susceptible strain. The 1014 F allele was fixed in the S-form and at high frequency in the M-form (0.7-0.914). The frequency of 1575Y allele was 0.29-0.36 in the S-form and nil in the M-form. The 1014S allele was detected in the S form of An. gambiae in a 1014 F/1014S heterozygous specimen.
  
  
  CONCLUSIONS
  Our results show that the kdr 1014 F, 1014S and 1575Y alleles are widespread in Benin and the expression of two candidate metabolic markers (GSTE2 and CYP6M2) are over-expressed specifically in the M-form.
authors.affiliation: Ecole Normale Supérieure de Natitingou, Université de Parakou, BP 123 Parakou, Benin. djegbe1@yahoo.fr.
authors.forename: Innocent
authors.initials: I
authors.lastname: Djègbè
authors.forename: Fiacre R
authors.initials: FR
authors.lastname: Agossa
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Jones
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Poupardin
authors.forename: Sylvie
authors.initials: S
authors.lastname: Cornelie
authors.forename: Martin
authors.initials: M
authors.lastname: Akogbéto
authors.forename: Hilary
authors.initials: H
authors.lastname: Ranson
authors.forename: Vincent
authors.initials: V
authors.lastname: Corbel
mesh_terms: D000818:Animals; D000852:Anopheles / Q000187:drug effects* / Q000235:genetics*; D001541:Benin; D003634:DDT / Q000494:pharmacology*; D018076:DNA, Complementary; D005786:Gene Expression Regulation; D005838:Genotype; D007305:Insecticide Resistance / Q000235:genetics*; D007306:Insecticides / Q000494:pharmacology*; D009154:Mutation; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D007306:Insecticides; D012333:RNA, Messenger; D012313:RNA; D003634:DDT