title: Physical and bioengineering properties of polyvinyl alcohol lens-shaped particles versus spherical polyelectrolyte complex microcapsules as immobilisation matrices for a whole-cell Baeyer-Villiger monooxygenase.
abstract: Direct comparison of key physical and chemical-engineering properties of two representative matrices for multipurpose immobilisations was performed for the first time. Polyvinyl alcohol lens-shaped particles LentiKats® and polyelectrolyte complex microcapsules were characterised by advanced techniques with respect to the size distribution of the particles, their inner morphology as revealed by fluorescent probe staining, mechanical resistance, size-exclusion properties, determination of effective diffusion coefficient and environmental scanning electron microscope imaging. While spherical polyelectrolyte complex microcapsules composed of a rigid semipermeable membrane and a liquid core are almost uniform in shape and size (diameter of 0.82 mm; RSD = 5.6 %), lens-shaped LentiKats® are characterised by wider size distribution (diameter of 3.65 mm; RSD = 10.3 % and height of 0.341 mm; RSD = 32.3 %) and showed the same porous structure throughout their whole volume at the mesoscopic (micrometre) level. Despite differences in their inner structure and surface properties, the pore diameter of ∼ 2.75 nm for regular polyelectrolyte complex microcapsules and ∼ 1.89 nm for LentiKats® were similar. These results were used for mathematical modelling, which provided the estimates of the effective diffusion coefficient of sucrose. This value was 1.67 × 10(-10) m(2) s(-1) for polyelectrolyte complex microcapsules and 0.36 × 10(-10) m(2) s(-1) for LentiKats®. Recombinant cells Escherichia coli-overexpressing enzyme cyclopentanone monooxygenase were immobilised in polyelectrolyte complex microcapsules and LentiKats® for comparison of their operational stability using model Baeyer-Villiger oxidation of (±)-cis-bicyclo [3.2.0] hept-2-en-6-one to regioisomeric lactones as important chiral synthons for potential pharmaceuticals. Both immobilisation matrices rendered high operational stability for whole-cell biocatalyst with no reduction in the biooxidation rate over 18 repeated reaction cycles.
authors.affiliation: Institute of Chemistry, Centre for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38, Bratislava, Slovakia, chemasch@savba.sk.
authors.forename: Andrea
authors.initials: A
authors.lastname: Schenkmayerová
authors.forename: Marek
authors.initials: M
authors.lastname: Bučko
authors.forename: Peter
authors.initials: P
authors.lastname: Gemeiner
authors.forename: Dušana
authors.initials: D
authors.lastname: Treľová
authors.forename: Igor
authors.initials: I
authors.lastname: Lacík
authors.forename: Dušan
authors.initials: D
authors.lastname: Chorvát
authors.forename: Pavel
authors.initials: P
authors.lastname: Ačai
authors.forename: Milan
authors.initials: M
authors.lastname: Polakovič
authors.forename: Lukáš
authors.initials: L
authors.lastname: Lipták
authors.forename: Martin
authors.initials: M
authors.lastname: Rebroš
authors.forename: Michal
authors.initials: M
authors.lastname: Rosenberg
authors.forename: Vladimír
authors.initials: V
authors.lastname: Stefuca
authors.forename: Vilém
authors.initials: V
authors.lastname: Neděla
authors.forename: Eva
authors.initials: E
authors.lastname: Tihlaříková
mesh_terms: D002214:Capsules; D004573:Electrolytes / Q000737:chemistry; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology*; D008422:Materials Testing; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*
chemical_list: D002214:Capsules; D004573:Electrolytes; D004800:Enzymes, Immobilized; D011142:Polyvinyl Alcohol; D010105:Oxygenases; C067215:cyclopentanone monooxygenase