title: In vitro\ex vivo generation of cytotoxic T lymphocytes.
abstract: The in vitro generation of human cytotoxic T lymphocytes (CTL) is a reliably approach useful not only to assess intrinsic CD8(+) T cell responses in individuals but also to screen immunogenic antigens that could be considered as candidates for adoptive immunotherapeutic approaches. In vitro methods to expand CTL require culturing naïve T cells with antigen-presenting cells (APC) as stimulator cells that express specific antigens. Here, we describe the protocol for generating CTL against target antigens presented by monocyte-derived dendritic cells (DCs).
authors.affiliation: Nephrology, Dialysis and Transplantation Unit, Department of Emergency and Organ Transplantation, Policlinico, University of Bari "A. Moro", Piazza G. Cesare 11, Bari, 70124, Italy, margherita.gigante@uniba.it.
authors.forename: Margherita
authors.initials: M
authors.lastname: Gigante
authors.forename: Elena
authors.initials: E
authors.lastname: Ranieri
mesh_terms: D000951:Antigens, Neoplasm / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D015789:HLA-A2 Antigen / Q000276:immunology; D006801:Humans; D016219:Immunotherapy, Adoptive; D015847:Interleukin-4 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology*; D009000:Monocytes / Q000166:cytology*; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology* / Q000276:immunology*
chemical_list: D000951:Antigens, Neoplasm; D017252:CD3 Complex; D015789:HLA-A2 Antigen; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor