title: Associations of IL-27 polymorphisms and serum IL-27p28 levels with osteosarcoma risk.
abstract: Interleukin (IL)-27 is a novel cytokine secreted by stimulation of antigen-presenting cells. No previous studies currently reported the role of IL-27 in the carcinogenesis of osteosarcoma. We aimed to investigate the association of IL-27 polymorphisms and serum IL-27p28 with osteosarcoma risk in a Chinese population.One hundred and sixty osteosarcoma patients and 250 health controls were selected. IL-27 gene -964 A/G, 2905 T/G, and 4730 T/C polymorphisms were determined by using polymerase chain reaction-restriction fragment length polymorphism. Enzyme-linked immunosorbent assay were used to detect serum IL-27p28 levels.The serum IL-27p28 levels were significantly lower in osteosarcoma patients compared with those in controls (P < 0.01). Serum IL-27p28 levels in stages III-IV were lower than those in stages I-II of osteosarcoma (P < 0.05); similar results were also found in patients with metastasis, that is, patients with metastasis have higher IL-27p28 levels than those without metastasis (P < 0.05). There were no associations between genotype and allele frequencies of IL-27 -964 A/G, 2905 T/G, 4730 T/C, and the risk of osteosarcoma (P > 0.05). Stratification analysis also failed to show the associations between -964 A/G, 2905 T/G, and 4730 T/C polymorphisms and the clinical stage and metastasis of osteosarcoma (P > 0.05). Three possible haplotypes (ATT, GTT, and GGC) were identified, but no associations were found between them and the osteosarcoma risk (P > 0.05).This study indicates that the lower serum IL-27p28 levels may be associated with development and progression of osteosarcoma, but IL-27 gene -964 A/G, 2905 T/G, and 4730 T/C polymorphisms and their haplotypes are not associated with osteosarcoma risk.
authors.affiliation: First Affiliated Hospital of Jinan University, Guangzhou (Y-JT, Z-GZ, P-HL); Center of Clinical Laboratory (Y-JT, C-GL, P-HL); and Affiliated Hospital of Youjiang Medical College for Nationalities (J-LW, L-GN), Baise, Guangxi, China.
authors.forename: Yu-Jin
authors.initials: YJ
authors.lastname: Tang
authors.forename: Jun-Li
authors.initials: JL
authors.lastname: Wang
authors.forename: Le-Gen
authors.initials: LG
authors.lastname: Nong
authors.forename: Chang-Gong
authors.initials: CG
authors.lastname: Lan
authors.forename: Zhen-Gang
authors.initials: ZG
authors.lastname: Zha
authors.forename: Pin-Hu
authors.initials: PH
authors.lastname: Liao
mesh_terms: D000293:Adolescent; D000328:Adult; D044466:Asian People; D001859:Bone Neoplasms / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D018664:Interleukin-12 / Q000097:blood; D064094:Interleukin-27 / Q000097:blood* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012516:Osteosarcoma / Q000097:blood / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D064094:Interleukin-27; D018664:Interleukin-12