title: Impact of bubble size on growth and CO2 uptake of Arthrospira (Spirulina) platensis KMMCC CY-007.
abstract: Optimisation of cyanobacterial cell productivity should consider the key factors light cycle and carbon source. We studied the influence of CO2 bubble size on carbon uptake and fixation, on basis of mRNA expression levels in Arthrospira platensis KMMCC CY-007 at 30°C (light intensity: 40μmolm(-2)s(-1); 1% CO2). Growth rate, carbon fixation and lipid accumulation were examined over 7days under fine bubble (FB) (100μm Ø) bulk bubble (BB) (5000μm Ø) and non-CO2 (NB) aeration. The low affinity CO2 uptake mRNA (NDH-I4 complex) was stronger expressed than the high affinity NDH-I3 complex (bicA and sbtA) under 1% CO2 and FB conditions, with no expression of bicA1 and sbtA1 after 4days. The high affinity CO2 uptake mRNA levels corresponded to biomass, carbon content and lipid accumulation, and increase in NDH-I3 complex (9.72-fold), bicA (5.69-fold), and sbtA (10.61-fold), compared to NB, or BB conditions.
authors.affiliation: Advanced Green Energy and Environment Institute (AGEE), Handong Global University, Pohang 791-708, Gyeongbuk, Republic of Korea.
authors.forename: Kisok
authors.initials: K
authors.lastname: Kim
authors.affiliation: Advanced Green Energy and Environment Institute (AGEE), Handong Global University, Pohang 791-708, Gyeongbuk, Republic of Korea.
authors.forename: Jaeho
authors.initials: J
authors.lastname: Choi
authors.affiliation: Advanced Green Energy and Environment Institute (AGEE), Handong Global University, Pohang 791-708, Gyeongbuk, Republic of Korea.
authors.forename: Yosep
authors.initials: Y
authors.lastname: Ji
authors.affiliation: Advanced Green Energy and Environment Institute (AGEE), Handong Global University, Pohang 791-708, Gyeongbuk, Republic of Korea.
authors.forename: Soyoung
authors.initials: S
authors.lastname: Park
authors.affiliation: School of Life Sciences, Handong Global University, Pohang 791-708, Gyeongbuk, Republic of Korea.
authors.forename: Hyungki
authors.initials: H
authors.lastname: Do
authors.affiliation: Advanced Green Energy and Environment Institute (AGEE), Handong Global University, Pohang 791-708, Gyeongbuk, Republic of Korea.
authors.forename: Cherwon
authors.initials: C
authors.lastname: Hwang
authors.affiliation: Advanced Green Energy and Environment Institute (AGEE), Handong Global University, Pohang 791-708, Gyeongbuk, Republic of Korea.
authors.forename: Bongju
authors.initials: B
authors.lastname: Lee
authors.affiliation: Advanced Green Energy and Environment Institute (AGEE), Handong Global University, Pohang 791-708, Gyeongbuk, Republic of Korea. Electronic address: wilhelm@woodapple.net.
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Holzapfel
mesh_terms: D000704:Analysis of Variance; D001426:Bacterial Proteins / Q000378:metabolism*; D002244:Carbon / Q000493:pharmacokinetics; D057486:Carbon Cycle / Q000502:physiology*; D002245:Carbon Dioxide / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D005453:Fluorescence; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D046912:Multiprotein Complexes / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D053545:Spirulina / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D046912:Multiprotein Complexes; D012333:RNA, Messenger; D002245:Carbon Dioxide; D002244:Carbon
keywords: Arthrospira (Spirulina) platensis; Bubble size; CO(2) uptake; Cyanobacteria