title: Cytotoxic effect of microbial biosurfactants against human embryonic kidney cancerous cell: HEK-293 and their possible role in apoptosis.
abstract: Two different microbial biosurfactants S9BS and CHBS were isolated from Lysinibacillus fusiformis S9 and Bacillus tequilensis CH. Cytotoxicity effect of these biosurfactants on human embryonic kidney cancerous cell (HEK-293) were studied with the help of 3-(4,5-dimethylthiazol-2yl-)-2, 5-diphenyl tetrazolium bromide (MTT) assay and morphological changes were observed under inverted microscope. The biosurfactants exhibited positive cytotoxic effect on HEK-293 cell line. It was found that LC50 of S9BS and CHBS were 75 and 100 μg ml(-1), respectively. Further cell cycle and apoptosis analysis of biosurfactant-treated HEK-293 cell line were done by FACS. In this study, cytotoxic effect of glycolipid biosurfactant against HEK-293 cell lines is reported for the first time. Mechanism towards increased membrane permeability of biosurfactant-treated cancer cell may be the incorporation of its lipid moiety into the plasma membrane leading to formation of pores and membrane disruption. Hence, these microbial biosurfactants can prove to be significant biomolecule for cancer treatment.
authors.affiliation: Academy of Scientific and Innovative Research, CSIR-Institute of Minerals and Materials Technology, Bhubaneswar, 751013, India, arunpradhan83@gmail.com.
authors.forename: Arun Kumar
authors.initials: AK
authors.lastname: Pradhan
authors.forename: Nilotpala
authors.initials: N
authors.lastname: Pradhan
authors.forename: Purusottam
authors.initials: P
authors.lastname: Mohapatra
authors.forename: Chanakya Nath
authors.initials: CN
authors.lastname: Kundu
authors.forename: Prasanna Kumar
authors.initials: PK
authors.lastname: Panda
authors.forename: Barada Kanta
authors.initials: BK
authors.lastname: Mishra
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001407:Bacillus / Q000145:classification / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D057809:HEK293 Cells; D006801:Humans; D007680:Kidney Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D007928:Lethal Dose 50; D013501:Surface-Active Agents / Q000008:administration & dosage*
chemical_list: D013501:Surface-Active Agents