title: Anabolic androgens affect the competitive interactions in cell migration and adhesion between normal mouse urothelial cells and urothelial carcinoma cells.
abstract: The urothelium is constantly rebuilt by normal urothelial cells to regenerate damaged tissues caused by stimuli in urine. However, the urothelial carcinoma cells expand the territory by aberrant growth of tumor cells, which migrate and occupy the damaged tissues to spread outside and disrupt the normal cells and organized tissues and form a tumor. Therefore, the interaction between normal urothelial cells and urothelial carcinoma cells affect the initiation and progression of urothelial tumors if normal urothelial cells fail to migrate and adhere to the damages sites to regenerate the tissues. Here, comparing normal murine urothelial cells with murine urothelial carcinoma cells (MBT-2), we found that normal cells had less migration ability than carcinoma cells. And in our co-culture system we found that carcinoma cells had propensity migrating toward normal urothelial cells and carcinoma cells had more advantages to adhere than normal cells. To reverse this condition, we used anabolic androgen, dihyrotestosterone (DHT) to treat normal cells and found that DHT treatment increased the migration ability of normal urothelial cells toward carcinoma cells and the adhesion capacity in competition with carcinoma cells. This study provides the base of a novel therapeutic approach by using anabolic hormone-enforced normal urothelial cells to regenerate the damage urothelium and defend against the occupancy of carcinoma cells to thwart cancer development and recurrence.
authors.affiliation: Sex Hormone Research Center, Departments of Urology and Medical Technology, Graduate Institute of Clinical Medical Science, China Medical University/Hospital, Taichung 404, Taiwan.
authors.forename: Chi-Ping
authors.initials: CP
authors.lastname: Huang
authors.affiliation: Sex Hormone Research Center, Departments of Urology and Medical Technology, Graduate Institute of Clinical Medical Science, China Medical University/Hospital, Taichung 404, Taiwan; Department of Urology, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung, Taiwan.
authors.forename: Teng-Fu
authors.initials: TF
authors.lastname: Hsieh
authors.affiliation: Sex Hormone Research Center, Departments of Urology and Medical Technology, Graduate Institute of Clinical Medical Science, China Medical University/Hospital, Taichung 404, Taiwan; Department of Urology, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung, Taiwan.
authors.forename: Chi-Cheng
authors.initials: CC
authors.lastname: Chen
authors.affiliation: Sex Hormone Research Center, Departments of Urology and Medical Technology, Graduate Institute of Clinical Medical Science, China Medical University/Hospital, Taichung 404, Taiwan.
authors.forename: Xiao-Fan
authors.initials: XF
authors.lastname: Hung
authors.affiliation: Sex Hormone Research Center, Departments of Urology and Medical Technology, Graduate Institute of Clinical Medical Science, China Medical University/Hospital, Taichung 404, Taiwan.
authors.forename: Ai-Lin
authors.initials: AL
authors.lastname: Yu
authors.affiliation: Sex Hormone Research Center, Departments of Urology and Medical Technology, Graduate Institute of Clinical Medical Science, China Medical University/Hospital, Taichung 404, Taiwan; George Whipple Lab for Cancer Research, Departments of Pathology, Urology and Radiation Oncology, University of Rochester Medical Center, Rochester, NY, United States.
authors.forename: Chawnshang
authors.initials: C
authors.lastname: Chang
authors.affiliation: Sex Hormone Research Center, Departments of Urology and Medical Technology, Graduate Institute of Clinical Medical Science, China Medical University/Hospital, Taichung 404, Taiwan. Electronic address: chshyr@mail.cmu.edu.tw.
authors.forename: Chih-Rong
authors.initials: CR
authors.lastname: Shyr
mesh_terms: D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D018920:Coculture Techniques; D015194:Diffusion Chambers, Culture; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D017930:Genes, Reporter; D018960:Hyaluronan Receptors / Q000235:genetics / Q000378:metabolism; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D061251:Primary Cell Culture; D019459:Urothelium / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000728:Androgens; D018960:Hyaluronan Receptors; D013196:Dihydrotestosterone; D008156:Luciferases
keywords: Adhesion; Anabolic; Androgen; Migration; Urothelial carcinoma cell; Urothelial cell