title: Establishing quality control ranges for temocillin following CLSI-M23-A3 guideline.
abstract: OBJECTIVE
  This study aimed to establish acceptable quality control ranges for temocillin disk diffusion tests and Etest(®) minimal inhibitory concentrations.
  
  
  METHODS
  According to Clinical and Laboratory Standards Institute (CLSI) guideline, a Tier 2 quality control study was performed and involves seven laboratories. Each of them tested 10 replicates of two quality control strains (Escherichia coli ATCC 25922 and E. coli ATCC 35218) on three different media lots and, for disk diffusion, two disk lots.
  
  
  RESULTS
  Proposed zone diameter quality control ranges were 12-25 mm for E. coli ATCC 25922 and 19-28 mm for E. coli ATCC 35218. Proposed Etest quality control ranges were 3-24 mg/l for E. coli ATCC 25922 and 2-6 mg/l E. coli ATCC 35218.
  
  
  CONCLUSIONS
  Based on our results, we would advise the use of E. coli ATCC 35218 as QC strain for temocillin susceptibility testing and Etest because ranges obtained are narrower than with E. coli ATCC 25922 and do not overlap temocillin breakpoint.
authors.forename: W
authors.initials: W
authors.lastname: Maurissen
authors.forename: G De
authors.initials: GD
authors.lastname: Sutter
authors.forename: H
authors.initials: H
authors.lastname: De Beenhouwer
authors.forename: J
authors.initials: J
authors.lastname: Frans
authors.forename: A-M
authors.initials: AM
authors.lastname: Van den Abeele
authors.forename: R
authors.initials: R
authors.lastname: Cartuyvels
authors.forename: W
authors.initials: W
authors.lastname: Laffut
authors.forename: P
authors.initials: P
authors.lastname: Vandecandelaere
authors.forename: G
authors.initials: G
authors.lastname: Coppens
mesh_terms: D052978:Disk Diffusion Antimicrobial Tests / Q000592:standards*; D004926:Escherichia coli; D010406:Penicillins; D011786:Quality Control; D012015:Reference Standards
chemical_list: D010406:Penicillins; C031367:temocillin
keywords: Etest; Quality control,; Susceptibility testing,; Temocillin,