title: Annona squamosa Linn: cytotoxic activity found in leaf extract against human tumor cell lines.
abstract: Cancer is a common cause of death in human populations. Surgery, chemotherapy and radiotherapy still remain the corner stone of treatment. However, herbal medicines are gaining popularity on account of their lesser harmful side effects on non-targeted human cells and biological environment. Annona squamosa Linn is a common delicious edible fruit and its leaf have been used for the treatment in various types of diseases. The objective of present study is to determine the anticancer potential of the organic and aqueous extracts of leaf of Annona squamosa L. MTT (3-(4, 5-dimethylthiazole-2yl)-2, 5-biphenyl tetrazolium bromide) assay against hepatocellular carcinoma cell line BEL-7404, lung cancer line H460, human epidermoid carcinoma cell line KB-3-1, prostatic cancer cell line DU145, breast carcinoma cell line MDA-MB-435, and colon cancer cell line HCT-116 Human primary embryonic kidney cell line HEK293 as control were used for the study. The crude extract (Zcd) and Ethyl acetate extract (ZE) were found significant anticancer activity only on human epidermoid carcinoma cell line KB-3-1 and colon cancer cell line HCT-116.
authors.affiliation: Department of Medical Oncology, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen Univesrity Cancer Center, Guanghzhou, China / Department of Pharmaceutical Science, College of Pharmacy and Health Sciences, St. John's University, Queen, UY, USA.
authors.forename: De-Shen
authors.initials: DS
authors.lastname: Wang
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan.
authors.forename: Ghazala H
authors.initials: GH
authors.lastname: Rizwani
authors.affiliation: Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing, China.
authors.forename: Huiqin
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan.
authors.forename: Mansoor
authors.initials: M
authors.lastname: Ahmed
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan.
authors.forename: Maryam
authors.initials: M
authors.lastname: Ahmed
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan.
authors.forename: Syed Zeeshan
authors.initials: SZ
authors.lastname: Hassan
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan.
authors.forename: Amir
authors.initials: A
authors.lastname: Hassan
authors.affiliation: Department of Pharmaceutical Science, College of Pharmacy and Health Sciences, St. John's University, Queen, UY, USA.
authors.forename: Zhe-Sheng
authors.initials: ZS
authors.lastname: Chen
authors.affiliation: Department of Medical Oncology, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen Univesrity Cancer Center, Guanghzhou, China.
authors.forename: Rui-Hua
authors.initials: RH
authors.lastname: Xu
mesh_terms: D000085:Acetates / Q000737:chemistry; D031041:Annona / Q000737:chemistry; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D045325:HCT116 Cells; D057809:HEK293 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves; D010946:Plants, Medicinal; D012997:Solvents / Q000737:chemistry
chemical_list: D000085:Acetates; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D012997:Solvents; C007650:ethyl acetate