title: Sub-proteome S-nitrosylation analysis in Brassica juncea hints at the regulation of Brassicaceae specific as well as other vital metabolic pathway(s) by nitric oxide and suggests post-translational modifications cross-talk.
abstract: Abiotic stress affects the normal physiology of the plants and results in crop loss. Brassica juncea is an oil yielding crop affected by abiotic stress. In future, over 30% yield loss by abiotic stress is predicted in India. Understanding the mechanism of plant response to stress would help in developing stress tolerant crops. Nitric oxide (NO) is now viewed as a remarkably important signaling molecule, involved in regulating stress responses. S-Nitrosylation is a NO based post-translational modification (PTM), linked with the regulation of many physiologically relevant targets. In the last decade, over 700 functionally varied S-nitrosylated proteins were identified, which suggested broad-spectrum regulation. To understand the physiological significance of S-nitrosylation, it was analyzed in cold stress. Functional categorization and validation of some of the B. juncea S-nitrosylated targets, suggested that NO produced during stress regulates cellular detoxification by modulating enzymes of ascorbate glutathione cycle, superoxide dismutase, glutathione S-transferase and glyoxalase I by S-nitrosylation in crude, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) depleted and apoplastic fractions. Interestingly, S-nitrosylation of enzymes associated with glucosinolate hydrolysis pathway, suggests a novel regulation of this Brassicaceae specific pathway by NO. Moreover, identification of enzymes of Glycolysis and Calvin cycle in crude and RuBisCO depleted fractions showed the regulation of metabolic as well as photosynthetic pathways by S-nitrosylation. S-Nitrosylation of cell wall modifying and proteolytic enzymes in the apoplast suggested differential and spatial regulation by S-nitrosylation. To have an overview of physiological role(s) of NO, collective information on NO based signaling (mainly by S-nitrosylation) is presented in this review.
authors.affiliation: Molecular Plant Physiology and Proteomics Laboratory, Department of Botany, University of Delhi, Delhi 110007, India.
authors.forename: Ankita
authors.initials: A
authors.lastname: Sehrawat
authors.affiliation: Molecular Plant Physiology and Proteomics Laboratory, Department of Botany, University of Delhi, Delhi 110007, India. Electronic address: rdeswal@botany.du.ac.in.
authors.forename: Renu
authors.initials: R
authors.lastname: Deswal
mesh_terms: D053858:Metabolic Networks and Pathways / Q000502:physiology; D009149:Mustard Plant / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D009589:Nitrogen Oxides / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D020543:Proteome; D015398:Signal Transduction
chemical_list: D009589:Nitrogen Oxides; D020543:Proteome; D009569:Nitric Oxide
keywords: Brassica juncea; Cold stress; Nitric oxide; S-nitrosylation; Thiol pool