title: miR-124a restoration inhibits glioma cell proliferation and invasion by suppressing IQGAP1 and β-catenin.
abstract: A number of microRNAs have been identified to be important regulators of tumorigenesis. Previous research has shown that miR-124 is abundantly expressed in normal brain tissue; however, only a few reports have focused on the biological impact of miR-124 on glioma cells, and the underlying mechanisms need to be elucidated. Therefore, we investigated the effect of miR-124a on glioma cell proliferation and invasion; furthermore, the underlying molecular mechanism was examined. The present study demonstrated that miR-124a expression was downregulated in human glioma tissues, and its expression level was negatively correlated with the pathological grade of the glioma. Restoration of miR-124a inhibited glioma cell proliferation and invasion in vitro. Furthermore, we found that miR-124a directly targeted and suppressed IQ motif containing GTPase activating protein 1 (IQGAP1), a well-known regulator of actin dynamics and cell motility. RNA interference assay showed that IQGAP1 knockdown led to downregulation of β-catenin and downstream cyclin D1. Taken together, our study revealed that miR-124a could inhibit glioma cell proliferation and invasion by blocking the expression of the IQGAP1 gene and downstream β-catenin and cyclin D1. This research may provide a useful molecular therapy for gliomas.
authors.affiliation: The Institute of Skull Base Surgery and Neurooncology at Hunan, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Lu
authors.affiliation: The Institute of Skull Base Surgery and Neurooncology at Hunan, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Xing-Jun
authors.initials: XJ
authors.lastname: Jiang
authors.affiliation: The Institute of Skull Base Surgery and Neurooncology at Hunan, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Ge-Lei
authors.initials: GL
authors.lastname: Xiao
authors.affiliation: The Institute of Skull Base Surgery and Neurooncology at Hunan, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Ding-Yang
authors.initials: DY
authors.lastname: Liu
authors.affiliation: The Institute of Skull Base Surgery and Neurooncology at Hunan, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Xian-Rui
authors.initials: XR
authors.lastname: Yuan
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D005910:Glioma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism; D020703:ras GTPase-Activating Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: C101203:IQ motif containing GTPase activating protein 1; C509207:MIRN124 microRNA, human; D035683:MicroRNAs; D051176:beta Catenin; D020703:ras GTPase-Activating Proteins