title: CNBP modulates the transcription of Wnt signaling pathway components.
abstract: BACKGROUND
  Cellular nucleic acid binding protein (CNBP) is a small and highly conserved protein with nucleic acid chaperone activity that binds single-stranded nucleic acids. Data collected so far suggests that CNBP is required for proper craniofacial development. Despite the advances achieved in the last decade, the identity of the molecular targets of CNBP responsible for its role in rostral head development remains elusive.
  
  
  METHODS
  In this work we used the CNBP single-stranded DNA-consensus binding sequence to find out putative CNBP target genes present in the human, mouse, chicken, Xenopus and zebrafish genomes.
  
  
  RESULTS
  Most of the identified genes are associated with embryonic developmental processes, being three of them (cdk14, ptk7 and tcf7l2) members of the Wnt signaling pathway. This finding, along with previous one showing that CNBP down-regulates the transcription of Wnt5, aimed our work to address the role of CNBP on the WNT signaling players and pathway regulation. Experiments carried out in zebrafish developing embryos revealed that craniofacial morphology was more adversely affected as CNBP abundance decreased. Furthermore, we observed that CNBP up-regulated in a dose-dependent fashion the transcription of cdk14, ptk7 and tcf7l2, which in turn was reflected in c-myc, ccnd1 and axin2 expression.
  
  
  CONCLUSIONS
  RESULTS reveal a role of CNBP in transcriptional control of components of the Wnt signaling pathway, which might explain its requirement for proper craniofacial development.
authors.affiliation: Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) - Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Ocampo y Esmeralda,(S2000FHQ) Rosario, Argentina.
authors.forename: Ezequiel
authors.initials: E
authors.lastname: Margarit
authors.affiliation: Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) - Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Ocampo y Esmeralda,(S2000FHQ) Rosario, Argentina.
authors.forename: Pablo
authors.initials: P
authors.lastname: Armas
authors.affiliation: Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) - Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Ocampo y Esmeralda,(S2000FHQ) Rosario, Argentina.
authors.forename: Nicolás
authors.initials: N
authors.lastname: García Siburu
authors.affiliation: Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) - Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Ocampo y Esmeralda,(S2000FHQ) Rosario, Argentina. Electronic address: calcaterra@ibr-conicet.gov.ar.
authors.forename: Nora B
authors.initials: NB
authors.lastname: Calcaterra
mesh_terms: D000818:Animals; D001846:Bone Development / Q000235:genetics; D002645:Chickens; D004625:Embryo, Nonmammalian; D005145:Face / Q000196:embryology; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051379:Mice; D016601:RNA-Binding Proteins / Q000502:physiology*; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics; D012886:Skull / Q000196:embryology; D058118:Transcription Factor 7-Like 2 Protein / Q000235:genetics; D060449:Wnt Signaling Pathway / Q000235:genetics*; D014981:Xenopus; D015027:Zebrafish / Q000196:embryology; D029961:Zebrafish Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D016601:RNA-Binding Proteins; C000589138:TCF7L2 protein, zebrafish; D058118:Transcription Factor 7-Like 2 Protein; D029961:Zebrafish Proteins; C499019:cnbpa protein, zebrafish; D020794:Receptor Protein-Tyrosine Kinases; C581480:ptk7b protein, zebrafish
keywords: Craniofacial development; Zebrafish; cdk14; cnbp; ptk7; tcf7l2