title: Production of structured phosphatidylcholine with high content of DHA/EPA by immobilized phospholipase A₁-catalyzed transesterification.
abstract: This paper presents the synthesis of structured phosphatidylcholine (PC) enriched with docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) by transesterification of DHA/EPA-rich ethyl esters with PC using immobilized phospholipsase A1 (PLA1) in solvent-free medium. Firstly, liquid PLA1 was immobilized on resin D380, and it was found that a pH of 5 and a support/PLA1 ratio (w/v) of 1:3 were the best conditions for the adsorption. Secondly, the immobilized PLA1 was used to catalyze transesterification of PC and DHA/EPA-rich ethyl esters. The maximal incorporation of DHA and EPA achieved was 30.7% for 24 h of reaction at 55 °C using a substrate mass ratio (PC/ethyl esters) of 1:6, an immobilized PLA1 loading of 15% and water dosage of 1.25%. Then the reaction mixture was analyzed by 31P nuclear magnetic resonance (NMR). The composition of reaction product included 16.5% PC, 26.3% 2-diacyl-sn-glycero-3-lysophosphatidylcholine (1-LPC), 31.4% 1-diacyl-sn-glycero-3-lysophosphatidylcholine (2-LPC), and 25.8% sn-glycerol-3-phosphatidylcholine (GPC).
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China. lxgryx@126.com.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China. chenjiafeng@163.com.
authors.forename: Jia-Feng
authors.initials: JF
authors.lastname: Chen
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China. yangbo@scut.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China. logangryx@163.com.
authors.forename: Dao-Ming
authors.initials: DM
authors.lastname: Li
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China. yonghw@scut.edu.cn.
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Wang
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China. biwfwang@scut.edu.cn.
authors.forename: Wei-Fei
authors.initials: WF
authors.lastname: Wang
mesh_terms: D004281:Docosahexaenoic Acids / Q000737:chemistry*; D015118:Eicosapentaenoic Acid / Q000737:chemistry*; D004800:Enzymes, Immobilized; D004951:Esterification; D008244:Lysophosphatidylcholines / Q000138:chemical synthesis / Q000737:chemistry; D010713:Phosphatidylcholines / Q000138:chemical synthesis* / Q000737:chemistry; D054466:Phospholipases A1 / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; D054466:Phospholipases A1