title: Endophytic fungi from Lycium chinense Mill and characterization of two new Korean records of Colletotrichum.
abstract: Chinese boxthorn or matrimony vine (Lycium chinense Mill) is found primarily in southeastern Europe and Asia, including Korea. The dried ripe fruits are commonly used as oriental medicinal purposes. Endophytic fungi were isolated from surface sterilized tissues and fruits of the medicinal plant in 2013 to identify the new or unreported species in Korea. Among 14 isolates, 10 morphospecies were selected for molecular identification with the internal transcribed spacer (ITS) gene. Phylogenetic analysis revealed that all isolates belonged to Ascomycota including the genera Acremonium, Colletotrichum, Cochliobolus, Fusarium, Hypocrea and Nemania. Two Colletotrichum species were identified at the species level, using three genes including internal transcribed spacer (ITS), glycerol-3-phosphate dehydrogenase (GAPDH) and Actin (ACT) for PCR and molecular data analysis along with morphological observations. The fungal isolates, CNU122031 and CNU122032 were identified as Colletotrichum fructicola and C. brevisporum, respectively. Morphological observations also well supported the molecular identification. C. brevisporum is represented unrecorded species in Korea and C. fructicola is the first record from the host plant.
authors.affiliation: Division of Applied Bioscience & Biotechnology, College of Agriculture & Life Sciences, Chonnam National University, Gwangju 500-757, Korea. ncpaul@jnu.ac.kr.
authors.forename: Narayan Chandra
authors.initials: NC
authors.lastname: Paul
authors.affiliation: Division of Applied Bioscience & Biotechnology, College of Agriculture & Life Sciences, Chonnam National University, Gwangju 500-757, Korea. hblee@jnu.ac.kr.
authors.forename: Hyang Burm
authors.initials: HB
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biology, College of Agriculture & Life Sciences, Chungnam National University, Daejeon 305-764, Korea. leejh224@nate.com.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biology, College of Agriculture & Life Sciences, Chungnam National University, Daejeon 305-764, Korea. seopbee@korea.kr.
authors.forename: Kyu Seop
authors.initials: KS
authors.lastname: Shin
authors.affiliation: Department of Agricultural Biology, College of Agriculture & Life Sciences, Chungnam National University, Daejeon 305-764, Korea. fbxogml89@naver.com.
authors.forename: Tae Hee
authors.initials: TH
authors.lastname: Ryu
authors.affiliation: Department of Agricultural Biology, College of Agriculture & Life Sciences, Chungnam National University, Daejeon 305-764, Korea. hr7345@nate.com.
authors.forename: Hye Ri
authors.initials: HR
authors.lastname: Kwon
authors.affiliation: Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration (RDA), Eumseung 369-873, Korea. kimyguk@korea.kr.
authors.forename: Yeong Kuk
authors.initials: YK
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biology, College of Agriculture & Life Sciences, Chungnam National University, Daejeon 305-764, Korea. youngnam@cnu.ac.kr.
authors.forename: Young Nam
authors.initials: YN
authors.lastname: Youn
authors.affiliation: Department of Agricultural Biology, College of Agriculture & Life Sciences, Chungnam National University, Daejeon 305-764, Korea. shunyu@cnu.ac.kr.
authors.forename: Seung Hun
authors.initials: SH
authors.lastname: Yu
mesh_terms: D000199:Actins / Q000235:genetics; D020231:Colletotrichum / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D021901:DNA, Intergenic / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000235:genetics; D032305:Lycium / Q000382:microbiology*; D010802:Phylogeny
chemical_list: D000199:Actins; D021901:DNA, Intergenic; D005656:Fungal Proteins; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)