title: Ribonomic approaches to study the RNA-binding proteome.
abstract: Gene expression is controlled through a complex interplay among mRNAs, non-coding RNAs and RNA-binding proteins (RBPs), which all assemble along with other RNA-associated factors in dynamic and functional ribonucleoprotein complexes (RNPs). To date, our understanding of RBPs is largely limited to proteins with known or predicted RNA-binding domains. However, various methods have been recently developed to capture an RNA of interest and comprehensively identify its associated RBPs. In this review, we discuss the RNA-affinity purification methods followed by mass spectrometry analysis (AP-MS); RBP screening within protein libraries and computational methods that can be used to study the RNA-binding proteome (RBPome).
authors.affiliation: School of Molecular Biosciences, University of Sydney, NSW, Australia.
authors.forename: Camilla
authors.initials: C
authors.lastname: Faoro
authors.affiliation: School of Molecular Biosciences, University of Sydney, NSW, Australia. Electronic address: sandro.ataide@sydney.edu.au.
authors.forename: Sandro F
authors.initials: SF
authors.lastname: Ataide
mesh_terms: D000818:Animals; D006801:Humans; D020543:Proteome / Q000737:chemistry* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D020543:Proteome; D016601:RNA-Binding Proteins; D012313:RNA
keywords: Non-coding RNAs (ncRNAs); RNA-binding proteins (RBPs); RNA-centric methods; RNA–protein interactions; Ribonome; Ribonucleoprotein particles (RNPs)