title: Tissue-specific mechanical and geometrical control of cell viability and actin cytoskeleton alignment.
abstract: Different tissues have specific mechanical properties and cells of different geometries, such as elongated muscle cells and polygonal endothelial cells, which are precisely regulated during embryo development. However, the mechanisms that underlie these processes are not clear. Here, we built an in vitro model to mimic the cellular microenvironment of muscle by combining both mechanical stretch and geometrical control. We found that mechanical stretch was a key factor that determined the optimal geometry of myoblast C2C12 cells under stretch, whereas vascular endothelial cells and fibroblasts had no such dependency. We presented the first experimental evidence that can explain why myoblasts are destined to take the elongated geometry so as to survive and maintain parallel actin filaments along the stretching direction. The study is not only meaningful for the research on myogenesis but also has potential application in regenerative medicine.
authors.affiliation: 1] Beijing Engineering Research Center for BioNanotechnology &CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190, China [2] Graduate University of the Chinese Academy of Sciences, Beijing 100846, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology &CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190, China.
authors.forename: Wenfu
authors.initials: W
authors.lastname: Zheng
authors.affiliation: 1] Beijing Engineering Research Center for BioNanotechnology &CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190, China [2] Graduate University of the Chinese Academy of Sciences, Beijing 100846, China.
authors.forename: Yunyan
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology &CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190, China.
authors.forename: Peiyuan
authors.initials: P
authors.lastname: Gong
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology &CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhao
authors.affiliation: 1] Beijing Engineering Research Center for BioNanotechnology &CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190, China [2] Graduate University of the Chinese Academy of Sciences, Beijing 100846, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yuan
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology &CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190, China.
authors.forename: Wanshun
authors.initials: W
authors.lastname: Ma
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology &CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cui
authors.affiliation: 1] Beijing Engineering Research Center for BioNanotechnology &CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190, China [2] Graduate University of the Chinese Academy of Sciences, Beijing 100846, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Liu
authors.affiliation: 1] Beijing Engineering Research Center for BioNanotechnology &CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190, China [2] Graduate University of the Chinese Academy of Sciences, Beijing 100846, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Biologie du cycle cellulaire et de la motilité, CNRS, Institut Curie, 26 rue d'Ulm 75248 Paris Cedex 05, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Piel
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology &CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology &CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing 100190, China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000199:Actins; D000818:Animals; D001696:Biomechanical Phenomena; D002460:Cell Line; D048430:Cell Shape; D002470:Cell Survival; D006801:Humans; D051379:Mice; D055550:Protein Stability; D059010:Single-Cell Analysis; D013314:Stress, Mechanical
chemical_list: D000199:Actins