title: Biologically active constituents from Salix viminalis bio-oil and their protective activity against hydrogen peroxide-induced oxidative stress in Chinese hamster ovary cells.
abstract: The protective antioxidative effect of the phenolic extract (PE) isolated from Salix viminalis pyrolysis derived bio-oil was shown in vitro on the Chinese hamster ovary (CHO) cells exposed to hydrogen peroxide (H2O2). Cells pretreated with 0.05 μg/ml PE after exposure to different concentrations of H2O2 (300-900 μM) showed up to 25 % higher viability than the unpretreated ones. The antioxidative effect of PE was also observed in a time-dependent manner. The results were confirmed by visual examination of the specimens using microscopy. Finally, superoxide dismutase (SOD) activity modulation was shown by SOD assay, designed to determine the activity of enzymes removing free radicals.
authors.affiliation: Faculty of Chemistry, Nicolaus Copernicus University, ul. Gagarina 7, 87-100, Torun, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Ilnicka
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Roszek
authors.forename: Andrzej
authors.initials: A
authors.lastname: Olejniczak
authors.forename: Michal
authors.initials: M
authors.lastname: Komoszynski
authors.forename: Jerzy P
authors.initials: JP
authors.lastname: Lukaszewicz
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D019800:Phenol / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry*; D032108:Salix / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D010938:Plant Oils; D019800:Phenol; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase