title: A method for the generation of TCR retrogenic mice.
abstract: Retrogenic mice provide a unique system for rapidly analyzing the function of genes in the hematopoietic system. Here, we provide a detailed protocol for the production of retrogenic mice expressing genes coding for T cell receptor (TCR) for antigen. This technology should be easy to establish in any laboratory and should allow for a rapid progress in our understanding of the functional roles of TCR repertoires in immunity.
authors.affiliation: Max Delbrueck Center for Molecular Medicine, Berlin, Germany.
authors.forename: Elisa
authors.initials: E
authors.lastname: Kieback
authors.forename: Ellen
authors.initials: E
authors.lastname: Hilgenberg
authors.forename: Simon
authors.initials: S
authors.lastname: Fillatreau
mesh_terms: D019264:Adoptive Transfer / Q000379:methods; D000818:Animals; D002478:Cells, Cultured; D018014:Gene Transfer Techniques; D006410:Hematopoiesis / Q000235:genetics; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D007111:Immunity, Cellular / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D012190:Retroviridae / Q000276:immunology
chemical_list: D011948:Receptors, Antigen, T-Cell