title: Dental pulp stem cells isolation and osteogenic differentiation: a good promise for tissue engineering.
abstract: Adult stem cells therapy can be an efficacious treatment for many diseases and disabilities. New sources of stem cells in adult organisms are continuously emerging. Dental tissues that are easily accessible by a tooth extraction have been identified as a source of postnatal mesenchymal stem cells capable of self-renewal and multipotency. Here, we describe accurately the technical procedure to isolate mesenchymal stem cells from dental pulp (DPSCs), characterize their immunophenotype, and assay their osteogenic capacity.
authors.affiliation: Department of Clinical and Experimental Medicine, University of Foggia, V.le L. Pinto 1, 71100, Foggia, Italy, adridibenedetto@libero.it.
authors.forename: Adriana
authors.initials: A
authors.lastname: Di Benedetto
authors.forename: Claudia
authors.initials: C
authors.lastname: Carbone
authors.forename: Giorgio
authors.initials: G
authors.lastname: Mori
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans; D007150:Immunohistochemistry; D010012:Osteogenesis; D061251:Primary Cell Culture; D044968:Regenerative Medicine; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering; D055815:Young Adult