title: Development of monoclonal antibodies to human kallikrein-related peptidase 6 (KLK6) and their use in an immunofluorometric assay for free KLK6.
abstract: We have raised monoclonal antibodies against KLK6 and constructed a 'sandwich' type immunoassay using 8A8G3 as capture and 3H3E9 as tracer antibodies. 8A8G3 bound to one side of KLK6, whereas 3H3E9 probably bound near its catalytic site. The assay did not detect complexed forms of KLK6, indicating that it quantifies only free KLK6 (fKLK6). fKLK6 was higher in serum of patients with ovarian cancer (11.34 μg/l±1.37) than in controls (3.39 μg/l±0.42). The cerebrospinal fluid contained high concentrations of fKLK6 (257-965 μg/l). This assay could facilitate the evaluation of fKLK6 in human diseases.
authors.forename: Catherine
authors.initials: C
authors.lastname: Ott
authors.forename: Mireille
authors.initials: M
authors.lastname: Ainciburu
authors.forename: Christelle
authors.initials: C
authors.lastname: Parent
authors.forename: Sandrine
authors.initials: S
authors.lastname: Michel
authors.forename: Céline
authors.initials: C
authors.lastname: Roesch
authors.forename: Patrick
authors.initials: P
authors.lastname: Vourc'h
authors.forename: Philippe
authors.initials: P
authors.lastname: Corcia
authors.forename: Nathalie
authors.initials: N
authors.lastname: Heuze-Vourc'h
authors.forename: Colette
authors.initials: C
authors.lastname: Jolivet-Reynaud
authors.forename: Yves
authors.initials: Y
authors.lastname: Courty
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001665:Binding Sites; D061505:Cell Surface Display Techniques; D018360:Crystallography, X-Ray; D005260:Female; D015200:Fluoroimmunoassay / Q000379:methods*; D006801:Humans; D007610:Kallikreins / Q000097:blood* / Q000737:chemistry / Q000276:immunology*; D008297:Male; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D010455:Peptides; C103551:KLK6 protein, human; D007610:Kallikreins