title: Isolation of blood-vessel-derived multipotent precursors from human skeletal muscle.
abstract: Since the discovery of mesenchymal stem/stromal cells (MSCs), the native identity and localization of MSCs have been obscured by their retrospective isolation in culture. Recently, using fluorescence-activated cell sorting (FACS), we and other researchers prospectively identified and purified three subpopulations of multipotent precursor cells associated with the vasculature of human skeletal muscle. These three cell populations: myogenic endothelial cells (MECs), pericytes (PCs), and adventitial cells (ACs), are localized respectively to the three structural layers of blood vessels: intima, media, and adventitia. All of these human blood-vessel-derived stem cell (hBVSC) populations not only express classic MSC markers but also possess mesodermal developmental potentials similar to typical MSCs. Previously, MECs, PCs, and ACs have been isolated through distinct protocols and subsequently characterized in separate studies. The current isolation protocol, through modifications to the isolation process and adjustments in the selective cell surface markers, allows us to simultaneously purify all three hBVSC subpopulations by FACS from a single human muscle biopsy. This new method will not only streamline the isolation of multiple BVSC subpopulations but also facilitate future clinical applications of hBVSCs for distinct therapeutic purposes.
authors.affiliation: Stem Cell Research Center, Department of Bioengineering and Orthopedic Surgery, University of Pittsburgh; chc88@pitt.edu.
authors.forename: William C W
authors.initials: WC
authors.lastname: Chen
authors.affiliation: Department of Orthopedic Surgery, University of Pittsburgh; Nazarbayev University Research and Innovation System, Nazarbayev University.
authors.forename: Arman
authors.initials: A
authors.lastname: Saparov
authors.affiliation: Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital and the Orthopaedic Hospital Research Center, University of California at Los Angeles.
authors.forename: Mirko
authors.initials: M
authors.lastname: Corselli
authors.affiliation: Department of Cell Biology, Erasmus MC Stem Cell Institute.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Crisan
authors.affiliation: OHSU Center for Regenerative Medicine, Oregon Health & Science University.
authors.forename: Bo
authors.initials: B
authors.lastname: Zheng
authors.affiliation: Centre for Cardiovascular Science and MRC Centre for Regenerative Medicine, Queen's Medical Research Institute and University of Edinburgh; David Geffen School of Medicine and the Orthopaedic Hospital Research Center, University of California at Los Angeles.
authors.forename: Bruno
authors.initials: B
authors.lastname: Péault
authors.affiliation: Stem Cell Research Center, Department of Orthopedic Surgery and McGowan Institute for Regenerative Medicine, University of Pittsburgh; jhuard@pitt.edu.
authors.forename: Johnny
authors.initials: J
authors.lastname: Huard
mesh_terms: D063194:Adventitia / Q000166:cytology*; D042783:Endothelial Cells / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D018482:Muscle, Skeletal / Q000098:blood supply*; D020286:Pericytes / Q000166:cytology*; D017539:Tunica Intima / Q000166:cytology*