title: Neutrophils prime a long-lived effector macrophage phenotype that mediates accelerated helminth expulsion.
abstract: We examined the role of innate cells in acquired resistance to the natural murine parasitic nematode, Nippostrongylus brasiliensis. Macrophages obtained from lungs as late as 45 d after N. brasiliensis inoculation were able to transfer accelerated parasite clearance to naive recipients. Primed macrophages adhered to larvae in vitro and triggered increased mortality of parasites. Neutrophil depletion in primed mice abrogated the protective effects of transferred macrophages and inhibited their in vitro binding to larvae. Neutrophils in parasite-infected mice showed a distinct transcriptional profile and promoted alternatively activated M2 macrophage polarization through secretory factors including IL-13. Differentially activated neutrophils in the context of a type 2 immune response therefore prime a long-lived effector macrophage phenotype that directly mediates rapid nematode damage and clearance.
authors.affiliation: 1] Center for Immunity and Inflammation, New Jersey Medical School, Rutgers-The State University of New Jersey, Newark, New Jersey, USA. [2] Department of Medicine, New Jersey Medical School, Rutgers-The State University of New Jersey, Newark, New Jersey, USA.
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.affiliation: 1] Center for Immunity and Inflammation, New Jersey Medical School, Rutgers-The State University of New Jersey, Newark, New Jersey, USA. [2] Department of Medicine, New Jersey Medical School, Rutgers-The State University of New Jersey, Newark, New Jersey, USA.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Wu
authors.affiliation: 1] Center for Immunity and Inflammation, New Jersey Medical School, Rutgers-The State University of New Jersey, Newark, New Jersey, USA. [2] Department of Medicine, New Jersey Medical School, Rutgers-The State University of New Jersey, Newark, New Jersey, USA.
authors.forename: Ariel
authors.initials: A
authors.lastname: Millman
authors.affiliation: Department of Medicine, Division of Experimental Medicine University of California, San Francisco, California, USA.
authors.forename: Joshua F
authors.initials: JF
authors.lastname: Craft
authors.affiliation: Department of Medicine, Division of Experimental Medicine University of California, San Francisco, California, USA.
authors.forename: Eunice
authors.initials: E
authors.lastname: Chen
authors.affiliation: 1] Center for Immunity and Inflammation, New Jersey Medical School, Rutgers-The State University of New Jersey, Newark, New Jersey, USA. [2] Department of Medicine, New Jersey Medical School, Rutgers-The State University of New Jersey, Newark, New Jersey, USA.
authors.forename: Nirav
authors.initials: N
authors.lastname: Patel
authors.affiliation: Laboratoire de Chimie et Biochimie, Pharmacologiques et Toxicologiques, Paris, France.
authors.forename: Jean L
authors.initials: JL
authors.lastname: Boucher
authors.affiliation: Beltsville Human Nutrition Research Center, Diet, Genomics, and Immunology Laboratory, US Department of Agriculture, Agricultural Research Service, Beltsville, Maryland, USA.
authors.forename: Joseph F
authors.initials: JF
authors.lastname: Urban
authors.affiliation: Department of Medicine, Division of Experimental Medicine University of California, San Francisco, California, USA.
authors.forename: Charles C
authors.identifier: 0000000164748227
authors.initials: CC
authors.lastname: Kim
authors.affiliation: 1] Center for Immunity and Inflammation, New Jersey Medical School, Rutgers-The State University of New Jersey, Newark, New Jersey, USA. [2] Department of Medicine, New Jersey Medical School, Rutgers-The State University of New Jersey, Newark, New Jersey, USA.
authors.forename: William C
authors.initials: WC
authors.lastname: Gause
mesh_terms: D056704:Adaptive Immunity / Q000276:immunology*; D000818:Animals; D002448:Cell Adhesion / Q000276:immunology / Q000502:physiology; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D060467:Disease Resistance / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006790:Host-Parasite Interactions / Q000276:immunology; D018793:Interleukin-13 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D053662:Interleukin-4 Receptor alpha Subunit / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007814:Larva / Q000276:immunology / Q000502:physiology; D008168:Lung / Q000276:immunology / Q000378:metabolism / Q000469:parasitology; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D009559:Nippostrongylus / Q000276:immunology* / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017206:Strongylida Infections / Q000235:genetics / Q000276:immunology* / Q000469:parasitology; D059467:Transcriptome / Q000276:immunology
chemical_list: D016207:Cytokines; D018793:Interleukin-13; D053662:Interleukin-4 Receptor alpha Subunit