title: Quantitative metabolomics using ID-MS.
abstract: Quantitative intracellular metabolite measurements are essential for systems biology and modeling of cellular metabolism. The MS-based quantification is error prone because (1) several sampling processing steps have to be performed, (2) the sample contains a complex mixture of partly compounds with the same mass and similar retention time, and (3) especially salts influence the ionization efficiency. Therefore internal standards are required, best for each measured compound. The use of labeled biomass, (13)C extract, is a valuable tool, reducing the standard deviations of intracellular concentration measurements significantly (especially regarding technical reproducibility). Using different platforms, i.e., LC-MS and GC-MS, a large number of different metabolites can be quantified (currently about 110).
authors.affiliation: Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands, s.a.wahl@tudelft.nl.
authors.forename: S Aljoscha
authors.initials: SA
authors.lastname: Wahl
authors.forename: Reza Maleki
authors.initials: RM
authors.lastname: Seifar
authors.forename: Angela
authors.initials: A
authors.lastname: Ten Pierick
authors.forename: Cor
authors.initials: C
authors.lastname: Ras
authors.forename: Jan C
authors.initials: JC
authors.lastname: van Dam
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Heijnen
authors.forename: Walter M
authors.initials: WM
authors.lastname: van Gulik
mesh_terms: D002247:Carbon Isotopes / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D064688:Metabolic Flux Analysis / Q000379:methods*; D055432:Metabolomics / Q000379:methods* / Q000706:statistics & numerical data
chemical_list: D002247:Carbon Isotopes