title: Study of amphotericin B magnetic liposomes for brain targeting.
abstract: This study aims to prepare amphotericin B magnetic liposomes (AmB-MLPs), which may improve drug concentration in brain, enhance magnetic targeting for brain and reduce drug toxicity in the presence of magnetic field. AmB-MLPs were prepared by means of film dispersion-ultrasonication, and their physical properties were characterized. In vivo, the magnetic targeting for brain by carotid artery administration was investigated. The particle size of AmB-MLPs was 240±11 nm, the encapsulation efficiency was 79.32±2.03%, and the saturation magnetization was 32.54 memu g⁻¹ at room temperature, which had good magnetic responsiveness. The group of AmB injection was delivered by carotid artery, nevertheless they all died after 20 min. AmB-MLPs were injected by carotid artery, and the drug concentration in brain tissue was obviously increased in presence of magnetic field than that of in absence of magnetic field (P<0.05). The Prussian blue staining in brain of SD rats showed that the density of blue staining-positive particles in brain tissue of applying magnetic field group was higher than that of non magnetic field group. These results suggested that AmB-MLPs could reinforce brain targeting and reduce drug toxicity when they were injected by carotid artery under the effect of magnetic field.
authors.affiliation: Department of Pharmacy, Hebei University of Science and Technology, Yuhua East Road 70, Shijiazhuang 050018, PR China.
authors.forename: Mengyu
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Immunology, Hebei Medical University, Zhongshan East Road 361, Shijiazhuang 050017, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Pharmacy, Hebei University of Science and Technology, Yuhua East Road 70, Shijiazhuang 050018, PR China.
authors.forename: Linan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutics, New Drug Research and Development Center, North China Pharmaceutical Group Corporation, Heping East Road 388, Shijiazhuang 050015, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Hebei University of Science and Technology, Yuhua East Road 70, Shijiazhuang 050018, PR China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Pharmacy, Hebei University of Science and Technology, Yuhua East Road 70, Shijiazhuang 050018, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Ma
authors.affiliation: Department of Pharmacy, Hebei University of Science and Technology, Yuhua East Road 70, Shijiazhuang 050018, PR China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, Hebei University of Science and Technology, Yuhua East Road 70, Shijiazhuang 050018, PR China; State Key Laboratory Breeding Base-Hebei Province Key Laboratory of Molecular Chemistry for Drug, PR China. Electronic address: zbgao74@163.com.
authors.forename: Zibin
authors.initials: Z
authors.lastname: Gao
mesh_terms: D000666:Amphotericin B / Q000008:administration & dosage* / Q000009:adverse effects / Q000378:metabolism / Q000493:pharmacokinetics; D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage* / Q000009:adverse effects / Q000378:metabolism / Q000493:pharmacokinetics; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000378:metabolism*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002339:Carotid Arteries; D002784:Cholesterol / Q000009:adverse effects / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000009:adverse effects / Q000378:metabolism / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000008:administration & dosage* / Q000009:adverse effects / Q000378:metabolism / Q000493:pharmacokinetics; D004339:Drug Compounding; D007275:Injections, Intravenous; D008081:Liposomes; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000009:adverse effects / Q000737:chemistry* / Q000648:ultrastructure; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D010316:Particle Size; D010713:Phosphatidylcholines / Q000009:adverse effects / Q000737:chemistry; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution; D015854:Up-Regulation
chemical_list: D000935:Antifungal Agents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D008081:Liposomes; D058185:Magnetite Nanoparticles; D010713:Phosphatidylcholines; D000666:Amphotericin B; D002784:Cholesterol
keywords: Amphotericin B; Brain targeting; Carotid artery administration; Magnetic liposomes