title: Escherichia coli as a glycoprotein production host: recent developments and challenges.
abstract: Chinese Hamster Ovary cells are the most popular host expression system for the large-scale production of human therapeutic glycoproteins, but, the race to engineer Escherichia coli to perform glycosylation is gathering pace. The successful functional transfer of an N-glycosylation pathway from Campylobacter jejuni to Escherichia coli in 2002 can be considered as the crucial first engineering step. Here, we discuss the recent advancements in the field of N-glycosylation of recombinant therapeutic proteins in E. coli cells, from the manipulation of glycan composition, to the improvement in glycosylation efficiency, along with the challenges that remain before E. coli can be available as an industry host cell for economically viable glycoprotein production.
authors.affiliation: ChELSI Institute, Department of Chemical and Biological Engineering, Sir Robert Hadfield Building, Mappin Street, Sheffield S1 3JD, UK.
authors.forename: Stephen R P
authors.initials: SR
authors.lastname: Jaffé
authors.affiliation: ChELSI Institute, Department of Chemical and Biological Engineering, Sir Robert Hadfield Building, Mappin Street, Sheffield S1 3JD, UK.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Strutton
authors.affiliation: Comenius University in Bratislava, Faculty of Natural Sciences, Department of Molecular Biology, Mlynská dolina, 842 15 Bratislava 4, Slovak Republic.
authors.forename: Zdenko
authors.initials: Z
authors.lastname: Levarski
authors.affiliation: ChELSI Institute, Department of Chemical and Biological Engineering, Sir Robert Hadfield Building, Mappin Street, Sheffield S1 3JD, UK.
authors.forename: Jagroop
authors.initials: J
authors.lastname: Pandhal
authors.affiliation: ChELSI Institute, Department of Chemical and Biological Engineering, Sir Robert Hadfield Building, Mappin Street, Sheffield S1 3JD, UK. Electronic address: p.c.wright@sheffield.ac.uk.
authors.forename: Phillip C
authors.initials: PC
authors.lastname: Wright
mesh_terms: D000818:Animals; D016466:CHO Cells; D016123:Campylobacter jejuni / Q000235:genetics; D003412:Cricetulus; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism* / Q000627:therapeutic use; D006031:Glycosylation; D006801:Humans; D007218:Industrial Microbiology; D011994:Recombinant Proteins / Q000378:metabolism* / Q000627:therapeutic use*
chemical_list: D006023:Glycoproteins; D011994:Recombinant Proteins