title: Removal of both Ycf48 and Psb27 in Synechocystis sp. PCC 6803 disrupts Photosystem II assembly and alters Q(A)(-) oxidation in the mature complex.
abstract: The Photosystem II (PS II) assembly factors Psb27 and Ycf48 are transiently associated with PS II during its biogenesis and repair pathways. We investigated the function of these proteins by constructing knockout mutants in Synechocystis sp. PCC 6803. In ΔYcf48 cells, PS II electron transfer and stable oxygen evolution were perturbed. Additionally, Psb27 was required for photoautotrophic growth of cells lacking Ycf48 and assembly beyond the RC47 assembly complex in ΔYcf48:ΔPsb27 cells was impeded. Our results suggest the RC47 complex formed in ΔYcf48 cells is defective and that this deficiency is exacerbated if CP43 binds in the absence of Psb27.
authors.affiliation: Department of Biochemistry, University of Otago, Dunedin 9016, New Zealand.
authors.forename: Simon A
authors.initials: SA
authors.lastname: Jackson
authors.affiliation: Department of Biochemistry, University of Otago, Dunedin 9016, New Zealand.
authors.forename: John R D
authors.initials: JR
authors.lastname: Hervey
authors.affiliation: Department of Biochemistry, University of Otago, Dunedin 9016, New Zealand.
authors.forename: Asher J
authors.initials: AJ
authors.lastname: Dale
authors.affiliation: Department of Biochemistry, University of Otago, Dunedin 9016, New Zealand. Electronic address: julian.eaton-rye@otago.ac.nz.
authors.forename: Julian J
authors.initials: JJ
authors.lastname: Eaton-Rye
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004237:Diuron / Q000494:pharmacology; D004579:Electron Transport; D017353:Gene Deletion; D045332:Photosystem II Protein Complex / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D055503:Protein Multimerization; D046939:Synechocystis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D045332:Photosystem II Protein Complex; C109473:orange carotenoid protein, Synechocystis; D004237:Diuron
keywords: Assembly; Biogenesis; Photosystem II; Psb27; Synechocystis sp. PCC 6803; Ycf48