title: Prediction of epitope-based peptides for the utility of vaccine development from fusion and glycoprotein of nipah virus using in silico approach.
abstract: This study aims to design epitope-based peptides for the utility of vaccine development by targeting glycoprotein G and envelope protein F of Nipah virus (NiV) that, respectively, facilitate attachment and fusion of NiV with host cells. Using various databases and tools, immune parameters of conserved sequence(s) from G and F proteins of different isolates of NiV were tested to predict probable epitope(s). Binding analyses of the peptides with MHC class-I and class-II molecules, epitope conservancy, population coverage, and linear B cell epitope prediction were analyzed. Predicted peptides interacted with seven or more MHC alleles and illustrated population coverage of more than 99% and 95%, for G and F proteins, respectively. The predicted class-I nonamers, SLIDTSSTI and EWISIVPNF, superimposed on the putative decameric B cell epitopes, were also identified as core sequences of the most probable class-II 15-mer peptides GPKVSLIDTSSTITI and EWISIVPNFILVRNT. These peptides were further validated for their binding to specific HLA alleles using in silico docking technique. Our in silico analysis suggested that the predicted epitopes, either GPKVSLIDTSSTITI or EWISIVPNFILVRNT, could be a better choice as universal vaccine component against NiV irrespective of different isolates which may elicit both humoral and cell-mediated immunity.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh.
authors.forename: M Sadman
authors.initials: MS
authors.lastname: Sakib
authors.affiliation: International Max Planck Research School for Neurosciences, University of Göttingen, 37077 Göttingen, Germany.
authors.forename: Md Rezaul
authors.initials: MR
authors.lastname: Islam
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh.
authors.forename: A K M Mahbub
authors.initials: AK
authors.lastname: Hasan
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh.
authors.forename: A H M Nurun
authors.initials: AH
authors.lastname: Nabi