title: miR-125b inhibits hepatitis B virus expression in vitro through targeting of the SCNN1A gene.
abstract: microRNAs (miRNAs) are small noncoding RNAs that modulate gene expression at the posttranscriptional level, playing an important role in many diseases. However, reports concerning the role of miRNA in hepatitis B virus (HBV) infection are limited. miRNA chips were used to investigate miRNA changes during HBV infection in vitro. Bioinformatics analysis was used to explore possible miRNA and target genes during HBV infection. The expression of miR-125b and its potential target gene, sodium channel, non-voltage-gated 1 alpha (SCNN1A), was further analyzed. A total of 136 miRNAs were analyzed in an HBV transient transfection model (HepG2-HBV1.3), and 78 miRNAs were differentially expressed in HepG2.2.15 cells compared with HepG2 cells. miR-125b expression was decreased in both HepG2-HBV1.3 and HepG2.2.15 cells, and ectopic expression of miR-125b inhibited HBV DNA intermediates and secretion of HBsAg and HBeAg. miR-125b also inhibited the mRNA and protein levels of SCNN1A. Using a dual luciferase reporter system, we found that SCNN1A was one of the targets of miR-125b. In this study, we found that miR-125b inhibits HBV expression in vitro by regulating SCNN1A expression.
authors.affiliation: Department of Infectious Disease, Children's Hospital of Chongqing Medical University, No 136 ZhongShan Er Road, 400012, Chongqing, China, 7522175@qq.com.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.forename: Yin
authors.initials: Y
authors.lastname: He
authors.forename: Xue
authors.initials: X
authors.lastname: Zhan
authors.forename: Ruiqiu
authors.initials: R
authors.lastname: Zhao
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Huang
authors.forename: Hongmei
authors.initials: H
authors.lastname: Xu
authors.forename: Zhaomin
authors.initials: Z
authors.lastname: Zhu
authors.forename: Quanbo
authors.initials: Q
authors.lastname: Liu
mesh_terms: D019295:Computational Biology; D053503:Epithelial Sodium Channels / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D020869:Gene Expression Profiling; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000276:immunology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*
chemical_list: D053503:Epithelial Sodium Channels; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; C507045:SCNN1A protein, human