title: Matrine alters microRNA expression profiles in SGC-7901 human gastric cancer cells.
abstract: Matrine, a major alkaloid extracted from Sophora flavescens, has been reported to possess antitumor properties in several types of cancers, including gastric cancer. However, its mechanisms of action on gastric cancer remain poorly understood. Dysregulation of microRNAs, a class of small, non-coding, regulatory RNA molecules involved in gene expression, is strongly correlated with cancer. The aim of the present study was to demonstrate that matrine treatment altered miRNA expression in SGC7901 cells. Using miRCURY™ microarray analysis, we identified 128 miRNAs substantially exhibiting >2-fold expression changes in matrine-treated cells relative to their expression levels in untreated cells. RT-qPCR was used to show that the levels of 8 miRNAs whose target genes were clustered in the cell cycle pathway increased, while levels of 14 miRNAs whose target genes were clustered in the MAPK signaling pathway decreased. These results were consistent with those from the miRNA microarray experiment. Bioinformatical analysis revealed that the majority of 57 identified enrichment pathways were highly involved in tumorigenesis. In conclusion, the results demonstrated that matrine induces considerable changes in the miRNA expression profiles of SGC7901 cells, suggesting miRNA microarray combined with RT-qPCR validation and bioinformatical analysis provide a novel and promising approach to identify anticancer targets and the mechanisms of matrine involved.
authors.affiliation: Key Laboratory of TCM Pharmacology and Toxicology of Gansu Province, Gansu Traditional Chinese Medical Univer, Lanzhou, Gansu 730000, P.R. Chinasity.
authors.forename: Hailong
authors.initials: H
authors.lastname: Li
authors.affiliation: First People's Hospital of Lanzhou City, Lanzhou, Gansu 730050, P.R. China.
authors.forename: Shoupin
authors.initials: S
authors.lastname: Xie
authors.affiliation: Oncology department of the first hospital of Lanzhou University, Lanzhou, Gansu 730000, P.R. China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of TCM Pharmacology and Toxicology of Gansu Province, Gansu Traditional Chinese Medical Univer, Lanzhou, Gansu 730000, P.R. Chinasity.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Wu
authors.affiliation: Key Laboratory of TCM Pharmacology and Toxicology of Gansu Province, Gansu Traditional Chinese Medical Univer, Lanzhou, Gansu 730000, P.R. Chinasity.
authors.forename: Xingyao
authors.initials: X
authors.lastname: Lin
authors.affiliation: Key Laboratory of TCM Pharmacology and Toxicology of Gansu Province, Gansu Traditional Chinese Medical Univer, Lanzhou, Gansu 730000, P.R. Chinasity.
authors.forename: Jing
authors.initials: J
authors.lastname: Gu
authors.affiliation: Key Laboratory of TCM Pharmacology and Toxicology of Gansu Province, Gansu Traditional Chinese Medical Univer, Lanzhou, Gansu 730000, P.R. Chinasity.
authors.forename: Huping
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of TCM Pharmacology and Toxicology of Gansu Province, Gansu Traditional Chinese Medical Univer, Lanzhou, Gansu 730000, P.R. Chinasity.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Duan
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects; D035683:MicroRNAs / Q000235:genetics*; D011807:Quinolizines / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000235:genetics*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D035683:MicroRNAs; D011807:Quinolizines; D000093842:Matrines