title: Genome-wide fungal stress responsive miRNA expression in wheat.
abstract: MicroRNAs (miRNAs) are small non-coding class of RNAs. They were identified in many plants with their diverse regulatory roles in several cellular and metabolic processes. A number of miRNAs were involved in biotic and abiotic stress responses. Here, fungal stress responsive wheat miRNAs were analyzed by using miRNA-microarray strategy. Two different fungi (Fusarium culmorum and Bipolaris sorokiniana) were inoculated on resistant and sensitive wheat cultivars. A total of 87 differentially regulated miRNAs were detected in the 8 × 15 K array including all of the available plant miRNAs. Using bioinformatics tools, the target transcripts of responsive miRNAs were predicted, and related biological processes and mechanisms were assessed. A number of the miRNAs such as miR2592s, miR869.1, miR169b were highly differentially regulated showing more than 200-fold change upon fungal-inoculation. Some of the miRNAs were identified as fungal-inoculation responsive for the first time. The analyses showed that some of the differentially regulated miRNAs targeted resistance-related genes such as LRR, glucuronosyl transferase, peroxidase and Pto kinase. The comparison of the two miRNA-microarray analyses indicated that fungal-responsive wheat miRNAs were differentially regulated in pathogen- and cultivar-specific manners.
authors.affiliation: Department of Biology, Faculty of Science, Cankiri Karatekin University, 18100, Cankiri, Turkey.
authors.forename: Behçet
authors.initials: B
authors.lastname: Inal
authors.forename: Mine
authors.initials: M
authors.lastname: Türktaş
authors.forename: Hakan
authors.initials: H
authors.lastname: Eren
authors.forename: Emre
authors.initials: E
authors.lastname: Ilhan
authors.forename: Sezer
authors.initials: S
authors.lastname: Okay
authors.forename: Mehmet
authors.initials: M
authors.lastname: Atak
authors.forename: Mustafa
authors.initials: M
authors.lastname: Erayman
authors.forename: Turgay
authors.initials: T
authors.lastname: Unver
mesh_terms: D001203:Ascomycota / Q000502:physiology*; D005670:Fusarium / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant; D018745:Genome, Plant; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D013312:Stress, Physiological / Q000235:genetics*; D014908:Triticum / Q000235:genetics* / Q000382:microbiology*
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger