title: GC-MS-based ¹³C metabolic flux analysis.
abstract: The in vivo analysis of metabolic fluxes has become a valuable method for the investigation of microorganisms. It turned out especially useful in industrial biotechnology for the prediction of beneficial genetic targets for rational strain optimization. Here, we describe in detail the procedure for the state-of-the-art approach of (13)C metabolic flux analysis comprising steady-state cultivations in a mineral salt medium with (13)C-labeled substrates, GC-MS measurement for labeling analysis, as well as metabolic modeling using the open-source software OpenFlux.
authors.affiliation: Institute of Systems Biotechnology, Saarland University, Campus A 1 5, 66123, Saarbrücken, Germany.
authors.forename: Judith
authors.initials: J
authors.lastname: Becker
authors.forename: Christoph
authors.initials: C
authors.lastname: Wittmann
mesh_terms: D002247:Carbon Isotopes / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007218:Industrial Microbiology / Q000379:methods*; D064688:Metabolic Flux Analysis / Q000295:instrumentation* / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000502:physiology*; D008954:Models, Biological
chemical_list: D002247:Carbon Isotopes