title: Investigation of the absorption of resveratrol oligomers in the Caco-2 cellular model of intestinal absorption.
abstract: Resveratrol oligomers are biologically active polyphenols found in wine. No information about the bioavailability of these polyphenols is available. In order to discover if the resveratrol oligomers can pass the intestinal barrier, transport of the dimer ε-viniferin and the tetramer hopeaphenol was studied in the Caco-2 transwell system. A flux through the cell monolayer could neither be observed for ε-viniferin nor for hopeaphenol (apparent permeability coefficient (Papp)<1×10(-6)cms(-1)). In contrast, resveratrol showed a Papp of 11.9×10(-6)cms(-1). Nevertheless, about 16-30% of the oligomers were found in the lysed cellular fraction. This leads to the conclusion that the intestinal absorption rate of the two resveratrol oligomers, ε-viniferin and hopeaphenol, is low and negligible when compared to resveratrol. Therefore, it is unlikely that the oligomers could elicit a systemic biological effect after dietary intake. However, the compounds may act locally on the intestinal epithelium.
authors.affiliation: University of Veterinary Medicine Hannover, Institute of Food Toxicology and Analytical Chemistry, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: Ina
authors.initials: I
authors.lastname: Willenberg
authors.affiliation: University of Veterinary Medicine Hannover, Institute of Food Toxicology and Analytical Chemistry, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Michael
authors.affiliation: University of Veterinary Medicine Hannover, Institute of Food Toxicology and Analytical Chemistry, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: Jasmin
authors.initials: J
authors.lastname: Wonik
authors.affiliation: University of Veterinary Medicine Hannover, Institute of Food Toxicology and Analytical Chemistry, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: Laura C
authors.initials: LC
authors.lastname: Bartel
authors.affiliation: University of Veterinary Medicine Hannover, Institute of Food Toxicology and Analytical Chemistry, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Empl
authors.affiliation: University of Veterinary Medicine Hannover, Institute of Food Toxicology and Analytical Chemistry, Bischofsholer Damm 15, 30173 Hannover, Germany. Electronic address: schebb@wwu.de.
authors.forename: Nils Helge
authors.initials: NH
authors.lastname: Schebb
mesh_terms: D001692:Biological Transport; D018938:Caco-2 Cells / Q000737:chemistry*; D006801:Humans; D007408:Intestinal Absorption / Q000502:physiology*; D059808:Polyphenols; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry*; D014920:Wine / Q000032:analysis
chemical_list: D059808:Polyphenols; D013267:Stilbenes; D000077185:Resveratrol
keywords: Automated sample preparation; Caco-2 transwell system; Hopeaphenol; Intestinal absorption; Resveratrol; Stilbene polyphenols; ε-Viniferin