title: A new bufadienolide with cytotoxic activity from the Chinese traditional drug Ch'an Su.
abstract: OBJECTIVE
  To study the bufadienolides in the Chinese traditional drug "Ch'an Su" and their cytotoxic activity.
  
  
  METHODS
  Various chromatographic techniques were used to isolate the constituents, and their structures were elucidated through physical and spectroscopic data.
  
  
  RESULTS
  Twenty compounds were isolated, and eighteen were evaluated in vitro for their cytotoxic activity against A-549 and K-562 cells.
  
  
  CONCLUSIONS
  Compound 1 (bufalin 3β-acrylic ester) was a new bufadienolide and exhibited the most potent activity against the two tumor cell lines with IC50 values of 7.16 and 6.83 nmol · L(-1). The relationships between structure and activity are discussed.
authors.affiliation: The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Xu
authors.affiliation: The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China.
authors.forename: Hai-Qin
authors.initials: HQ
authors.lastname: Xie
authors.affiliation: The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China.
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Deng
authors.affiliation: The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China.
authors.forename: Fu-Mei
authors.initials: FM
authors.lastname: Yang
authors.affiliation: The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Liu
authors.affiliation: The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China.
authors.forename: Xiao-Jiang
authors.initials: XJ
authors.lastname: Hao
authors.affiliation: The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China. Electronic address: zhangyuanhu@263.net.
authors.forename: Yuan-Hu
authors.initials: YH
authors.lastname: Zhang
mesh_terms: D000664:Amphibian Venoms / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D002018:Bufanolides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D020128:Inhibitory Concentration 50; D020014:K562 Cells; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D009369:Neoplasms / Q000188:drug therapy*; D013329:Structure-Activity Relationship
chemical_list: D000664:Amphibian Venoms; D000970:Antineoplastic Agents; D001688:Biological Products; D002018:Bufanolides; C000597411:bufalin 3beta-acrylic ester; C095591:chan su
keywords: Bufalin 3β-acrylic ester; Cytotoxicity; New bufadienolide; “Ch'an Su”