title: Lemongrass-Incorporated Tissue Conditioner Against Candida albicans Culture.
abstract: BACKGROUND
  Tissue conditioner is applied popularly with dental prosthesis during wound healing process but it becomes a reservoir of oral microbiota, especially Candida species after long-term usage. Several antifungal drugs have been mixed with this material to control fungal level. In this study, lemongrass essential oil was added into COE-COMFORT tissue conditioner before being determined for anti-Candida efficacy.
  
  
  METHODS
  Lemongrass (Cymbopogon citratus) essential oil was primarily determined for antifungal activity against C. albicans American type culture collection (ATCC) 10231 and MIC (minimum inhibitory concentration) value by agar disk diffusion and broth microdilution methods, respectively. COE-COMFORT tissue conditioner was prepared as recommended by the manufacturer after a fixed volume of the oil at its MIC or higher concentrations were mixed thoroughly in its liquid part. Antifungal efficacy of the tissue conditioner with/without herb was finally analyzed.
  
  
  RESULTS
  Lemongrass essential oil displayed potent antifungal activity against C. albicans ATCC 10231and its MIC value was 0.06% (v/v). Dissimilarly, the tissue conditioner containing the oil at MIC level did not cease the growth of the tested fungus. Both reference and clinical isolates of C. albicans were completely inhibited after exposed to the tissue conditioner containing at least 0.25% (v/v) of the oil (approximately 4-time MIC). The tissue conditioner without herb or with nystatin was employed as negative or positive control, respectively.
  
  
  CONCLUSIONS
  COE-COMFORT tissue conditioner supplemented with lemongrass essential oil obviously demonstrated another desirable property as in vitro anti-Candida efficacy to minimize the risk of getting Candidal infection.
authors.affiliation: Maxillofacial Prosthodontist, Maxillofacial Prosthetic Clinic, Department of Prosthodontics, Faculty of Dentistry, Mahidol University , Bangkok, Thailand .
authors.forename: Pokpong
authors.initials: P
authors.lastname: Amornvit
authors.affiliation: Assistant Professor, Department of Oral Microbiology, Faculty of Dentistry, Mahidol University , Bangkok, Thailand .
authors.forename: Suwan
authors.initials: S
authors.lastname: Choonharuangdej
authors.affiliation: Assistant Professor, Maxillofacial Prosthetic Clinic, Department of Prosthodontics, Faculty of Dentistry, Mahidol University , Bangkok, Thailand .
authors.forename: Theerathavaj
authors.initials: T
authors.lastname: Srithavaj
keywords: Candida albicans; Coe-comfort tissue conditioner; Lemongrass essential oil; Minimum inhibitory concentration (MIC)