title: Association of JARID2 polymorphisms with non-syndromic orofacial clefts in northern Chinese Han population.
abstract: OBJECTIVE
  Non-syndromic orofacial clefts (NSOC) are the most common human craniofacial malformation in all worldwide populations. Recently, the jumoji AT-rich interaction domain 2 (JARID2) had been reported to be a novel candidate gene for non-syndromic cleft lip with or without cleft palate (CL/P). The SNPs rs2076056, rs2237138 and rs2299043 in JARID2 were highly significant in Italian families.
  
  
  METHODS
  In the current research, a case-control study was conducted to examine the association between these three SNPs and NSOC in a northern Chinese Han population. Genotyping of the three SNPs were performed using SNaPshot minisequencing technique.
  
  
  RESULTS
  Distribution of rs2237138 genotypes in CL/P group was different from those in the control group (P = 0.04), but significant results did not persist after Benjamini and Hochberg false discovery rate (FDR) correction for multiple tests. Further logistic regression analysis showed that rs2237138 GG genotypes were associated with decreased CL/P susceptibility (OR = 0.34, 95% CI = 0.13-0.84), compared with the AA wild-type homozygote. For the haplotype CGT, a statistically difference was identified between the CL/P group and controls (P = 0.04). And carriers of GAT haplotype were considered to be less frequent among cleft palate only group as compared to controls (P = 0.02). However, both of the haplotypes association did not remain statistically significant after Benjamini and Hochberg FDR correction.
  
  
  CONCLUSIONS
  We got a weak association between these polymorphisms and NSOC in both single-marker and haplotype analyses. Our data further strengthen the conclusion that JARID2 polymorphisms are associated with NSOC susceptibility.
authors.affiliation: Department of Oral Maxillofacial Surgery, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Yanru
authors.initials: Y
authors.lastname: Hao
authors.forename: Na
authors.initials: N
authors.lastname: Mi
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Jiao
authors.forename: Xudong
authors.initials: X
authors.lastname: Zheng
authors.forename: Tao
authors.initials: T
authors.lastname: Song
authors.forename: Deshu
authors.initials: D
authors.lastname: Zhuang
authors.forename: Subao
authors.initials: S
authors.lastname: Tian
authors.forename: Dongfei
authors.initials: D
authors.lastname: Feng
mesh_terms: D001921:Brain / Q000002:abnormalities*; D016022:Case-Control Studies; D002681:China; D002971:Cleft Lip / Q000235:genetics*; D002972:Cleft Palate / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008297:Male; D063151:Polycomb Repressive Complex 2 / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
chemical_list: C547777:JARID2 protein, human; D063151:Polycomb Repressive Complex 2
keywords: JARID2; SNaPshot; association study; non-syndromic orofacial clefts; polymorphism