title: Optimization of ELFSE DNA sequencing with EOF counterflow and microfluidics.
abstract: We present a nonlinear optimization study of different implementations of the DNA electrophoretic method "End-labeled Free-solution Electrophoresis" in commercial capillary electrophoresis systems and microfluidics to improve the time required for readout. Here, the effect of electro-osmotic counterflows and snap-shot detection are considered to allow for detection of peaks soon after they are electorphoretically resolved. Using drag tags available in micelle form, we identify a design capable of sequencing 600 bases in 2.8 min.
authors.affiliation: Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.
authors.forename: Max A
authors.initials: MA
authors.lastname: Fahrenkopf
authors.forename: Tamal
authors.initials: T
authors.lastname: Mukherjee
authors.forename: B Erik
authors.initials: BE
authors.lastname: Ydstie
authors.forename: James W
authors.initials: JW
authors.lastname: Schneider
mesh_terms: D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013997:Time Factors
chemical_list: D004247:DNA
keywords: DNA electrophoresis; DNA sequencing; ELFSE; Gel-free; Rapid