title: Simultaneous determination of rivaroxaban and dabigatran levels in human plasma by high-performance liquid chromatography-tandem mass spectrometry.
abstract: A sensitive and accurate liquid chromatography method with mass spectrometry detection was developed and validated for the quantification of dabigatran (Pradaxa(®)) and rivaroxaban (Xarelto(®)). (13)C6-dabigatran and (13)C6-rivaroxaban were used as the internal standard. A single-step protein precipitation was used for plasma sample preparation. This method was validated with respect to linearity, selectivity, inter- and intra-day precision and accuracy, limit of quantification and stability. The lower limit of quantification was 2.5ng/mL for both drugs in plasma.
authors.affiliation: AP-HP, Pitié-Salpêtrière Hospital, Department of Pharmacology and CIC-1421, F-75013 Paris, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Korostelev
authors.affiliation: AP-HP, Pitié-Salpêtrière Hospital, Department of Pharmacology and CIC-1421, F-75013 Paris, France.
authors.forename: Kevin
authors.initials: K
authors.lastname: Bihan
authors.affiliation: AP-HP, Pitié-Salpêtrière Hospital, Department of Pharmacology and CIC-1421, F-75013 Paris, France.
authors.forename: Lison
authors.initials: L
authors.lastname: Ferreol
authors.affiliation: AP-HP, Pitié-Salpêtrière Hospital, Department of Pharmacology and CIC-1421, F-75013 Paris, France.
authors.forename: Nadine
authors.initials: N
authors.lastname: Tissot
authors.affiliation: AP-HP, Pitié-Salpêtrière Hospital, Department of Pharmacology and CIC-1421, F-75013 Paris, France; Sorbonne University, UPMC Univ Paris 06, Faculty of Medicine, Department of Pharmacology and UMR ICAN 1166, F-75013 Paris, France; INSERM, CIC-1421 and UMR ICAN 1166, F-75013 Paris, France.
authors.forename: Jean-Sebastien
authors.initials: JS
authors.lastname: Hulot
authors.affiliation: AP-HP, Pitié-Salpêtrière Hospital, Department of Pharmacology and CIC-1421, F-75013 Paris, France; Sorbonne University, UPMC Univ Paris 06, Faculty of Medicine, Department of Pharmacology and UMR ICAN 1166, F-75013 Paris, France; INSERM, CIC-1421 and UMR ICAN 1166, F-75013 Paris, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Funck-Brentano
authors.affiliation: AP-HP, Pitié-Salpêtrière Hospital, Department of Pharmacology and CIC-1421, F-75013 Paris, France. Electronic address: noel.zahr@psl.aphp.fr.
authors.forename: Noël
authors.initials: N
authors.lastname: Zahr
mesh_terms: D000991:Antithrombins / Q000097:blood*; D001562:Benzimidazoles / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D003080:Cold Temperature; D000069604:Dabigatran; D016903:Drug Monitoring / Q000379:methods* / Q000592:standards; D004355:Drug Stability; D065427:Factor Xa Inhibitors / Q000097:blood*; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D015394:Molecular Structure; D009025:Morpholines / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D000069552:Rivaroxaban; D053719:Tandem Mass Spectrometry / Q000592:standards; D013876:Thiophenes / Q000097:blood*; D013997:Time Factors; D015091:beta-Alanine / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D000991:Antithrombins; D001562:Benzimidazoles; D065427:Factor Xa Inhibitors; D009025:Morpholines; D013876:Thiophenes; D015091:beta-Alanine; D000069552:Rivaroxaban; D000069604:Dabigatran
keywords: Dabigatran; LC–MS/MS; Mass spectrometry; Rivaroxaban; TSOAC