title: The Antibacterial Activity of Cassia fistula Organic Extracts.
abstract: BACKGROUND
  Cassia fistula, is a flowering plant and a member of Fabaceae family. Its leaves are compound of 4 - 8 pairs of opposite leaflets. There are many Cassia species around the world which are used in herbal medicine.
  
  
  OBJECTIVE
  This study was designed to examine in vitro anti-bacterial activity of methanolic and ethanolic extracts of C. fistula native to Khuzestan, Iran.
  
  
  METHODS
  The microbial inhibitory effect of methanolic and ethanolic extracts of C. fistula was tested on 3 Gram positive: Bacillus cereus, Staphylococcus aureus and S. epidermidis and 5 Gram negative: Salmonella Typhi, Kelebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis bacterial species using disc diffusion method at various concentrations. The minimum inhibitory and bactericidal concentrations (MIC and MBC) were measured by the tube dilution assay.
  
  
  RESULTS
  The extract of C. fistula was effective against B. cereus, S. aureus, S. epidermidis, E. coli and K. pneumoniae. The most susceptible microorganisms to ethanolic and methanolic extracts were E. coli and K. pneumoniae, respectively. Also B. cereus and S. aureus showed the least sensitivity to ethanolic and methanolic extracts, respectively. The MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) of ethanolic extracts against S. aureus, E. coli, S. epidermidis and K. pneumoniae were also determined.
  
  
  CONCLUSIONS
  With respect to the obtained results and regarding to the daily increase of the resistant microbial strains to the commercial antibiotics, it can be concluded that these extracts can be proper candidates of antibacterial substance against pathogenic bacterial species especially S. aureus, E. coli, K. pneumoniae and S. epidermidis.
authors.affiliation: Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz, IR Iran.
authors.forename: Seyyed Mansour
authors.initials: SM
authors.lastname: Seyyednejad
authors.affiliation: Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz, IR Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Motamedi
authors.affiliation: Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz, IR Iran.
authors.forename: Mouzhan
authors.initials: M
authors.lastname: Vafei
authors.affiliation: Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz, IR Iran.
authors.forename: Ameneh
authors.initials: A
authors.lastname: Bakhtiari
keywords: Anti-bacterial Activity; Cassia fistula; Disc Diffusion Antibacterial Test; Minimum Bactericidal Concentration; Minimum Inhibitory Concentration