title: Production and characterization of a monoclonal antibody against GRAM domain-containing protein 1A.
abstract: From the proteomic analysis, we identified hundreds of novel proteins that have never been characterized for their expression profile and function on human embryonic stem (hES) cells. In this study, we produced a group of monoclonal antibodies against the GRAM domain-containing protein 1A, which was found on hES cells. Using these antibodies, we analyzed the expression of GRAMD1A in various tissues and tumor cell lines. The results showed that GRAMD1A is expressed in the nucleus and cytoplasm of hES cells, cancer cell lines and ectoderm, mesoderm, and endoderm tissues. The development of the monoclonal antibody to GRAMD1A and the characterization of the expression pattern of this protein could have significant implications in the functional characterization of this novel protein.
authors.affiliation: 1 Institute of Genetics, College of Life Sciences, Zhejiang University , Hangzhou, China .
authors.forename: Xiuli
authors.initials: X
authors.lastname: Song
authors.forename: Shihao
authors.initials: S
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Gu
authors.forename: Qiannv
authors.initials: Q
authors.lastname: Hou
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D053595:Embryonic Stem Cells / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005855:Germ Layers / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D040901:Proteomics; D011994:Recombinant Proteins / Q000276:immunology*; D012394:Rosaniline Dyes; D017422:Sequence Analysis, DNA
chemical_list: D000911:Antibodies, Monoclonal; D017931:DNA Primers; C000597919:GRAMD1A protein, human; D008565:Membrane Proteins; D011994:Recombinant Proteins; D012394:Rosaniline Dyes; C048139:Coomassie blue