title: Tailored PVA/ECM scaffolds for cartilage regeneration.
abstract: Articular cartilage lesions are a particular challenge for regenerative medicine due to cartilage low self-ability repair in case of damage. Hence, a significant goal of musculoskeletal tissue engineering is the development of suitable structures in virtue of their matrix composition and biomechanical properties. The objective of our study was to design in vitro a supporting structure for autologous chondrocyte growth. We realized a biohybrid composite scaffold combining a novel and nonspecific extracellular matrix (ECM), which is decellularized Wharton's jelly ECM, with the biomechanical properties of the synthetic hydrogel polyvinyl alcohol (PVA). Wharton's jelly ECM was tested for its ability in promoting scaffold colonization by chondrocytes and compared with polyvinyl alcohol itself and the more specific decellularized cartilage matrix. Our preliminary evidences highlighted the chance of using Wharton's jelly ECM in combination with PVA hydrogels as an innovative and easily available scaffold for cartilage restoration.
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Via Marzolo 5, 35131 Padua, Italy.
authors.forename: Elena
authors.identifier: 0000-0003-4572-622X
authors.initials: E
authors.lastname: Stocco
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Via Marzolo 5, 35131 Padua, Italy ; Foundation for Biology and Regenerative Medicine, Tissue Engineering and Signaling (TES) ONLUS, Via De Sanctis 10, Caselle di Selvazzano Dentro, 35030 Padua, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Barbon
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Via Marzolo 5, 35131 Padua, Italy.
authors.forename: Daniele
authors.identifier: 0000-0001-5896-7108
authors.initials: D
authors.lastname: Dalzoppo
authors.affiliation: Foundation for Biology and Regenerative Medicine, Tissue Engineering and Signaling (TES) ONLUS, Via De Sanctis 10, Caselle di Selvazzano Dentro, 35030 Padua, Italy.
authors.forename: Silvano
authors.initials: S
authors.lastname: Lora
authors.affiliation: Operative Unit of Plastic Surgery, Sant'Anna Hospital, Via Ravona 20, 22020 Como, Italy.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Sartore
authors.affiliation: Department of Biology, University of Padua, Viale Colombo 3, 35121 Padua, Italy.
authors.forename: Marcella
authors.initials: M
authors.lastname: Folin
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Via Marzolo 5, 35131 Padua, Italy ; Foundation for Biology and Regenerative Medicine, Tissue Engineering and Signaling (TES) ONLUS, Via De Sanctis 10, Caselle di Selvazzano Dentro, 35030 Padua, Italy.
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Parnigotto
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Via Marzolo 5, 35131 Padua, Italy.
authors.forename: Claudio
authors.identifier: 0000-0002-7736-2873
authors.initials: C
authors.lastname: Grandi
mesh_terms: D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology; D019902:Chondrocytes / Q000378:metabolism / Q000502:physiology; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D020100:Hydrogels / Q000494:pharmacology; D011142:Polyvinyl Alcohol / Q000494:pharmacology*; D012038:Regeneration / Q000502:physiology; D044968:Regenerative Medicine; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014470:Umbilical Cord / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D059631:Wharton Jelly / Q000187:drug effects / Q000378:metabolism / Q000502:physiology
chemical_list: D020100:Hydrogels; C062364:polyvinyl alcohol hydrogel; D011142:Polyvinyl Alcohol