title: Rapid analysis of raw solution samples by C18 pipette-tip electrospray ionization mass spectrometry.
abstract: A C18 pipette-tip electrospray ionization mass spectrometry technique was developed for rapid analysis of raw solution samples. In this technique, a C18 pipette tip was employed for rapid purification and enrichment of analytes in raw sample solutions. The adsorbed analytes were eluted by solvents supplied by a syringe and a syringe pump, and a high voltage was applied onto the syringe needle to induce electrospray ionization at the pipette tip end for mass spectrometric analysis. This technique is simple, easy to assemble, enables generation of stable and reproducible signals, and can be conveniently used for qualitative and quantitative analysis of raw solution samples. Analysis by the technique only involved simple sample preparation procedures followed by direct mass spectrometric detection, all of which could be completed within minutes, while the analytical performances of the technique, including the limit of detection, limit of quantitation, liner range, accuracy and precision, were comparable to those by conventional methods.
authors.affiliation: State Key Laboratory of Chirosciences, Food Safety and Technology Research Centre and Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Special Administrative Region, China; State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Shenzhen Research Institute of The Hong Kong Polytechnic University, Shenzhen 518057, China.
authors.forename: Haixing
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Chirosciences, Food Safety and Technology Research Centre and Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Special Administrative Region, China; State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Shenzhen Research Institute of The Hong Kong Polytechnic University, Shenzhen 518057, China.
authors.forename: Pui-Kin
authors.initials: PK
authors.lastname: So
authors.affiliation: State Key Laboratory of Chirosciences, Food Safety and Technology Research Centre and Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Special Administrative Region, China; State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Shenzhen Research Institute of The Hong Kong Polytechnic University, Shenzhen 518057, China.
authors.forename: Tsz-Tsun
authors.initials: TT
authors.lastname: Ng
authors.affiliation: State Key Laboratory of Chirosciences, Food Safety and Technology Research Centre and Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Special Administrative Region, China; State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Shenzhen Research Institute of The Hong Kong Polytechnic University, Shenzhen 518057, China. Electronic address: zhongping.yao@polyu.edu.hk.
authors.forename: Zhong-Ping
authors.initials: ZP
authors.lastname: Yao
mesh_terms: D004864:Equipment and Supplies; D057230:Limit of Detection; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation*
keywords: C18; Electrospray ionization mass spectrometry; Ketamine; Pipette tips; Proteins; Raw solutions