title: Downregulation of phosphoglycerate kinase 1 by shRNA sensitizes U251 xenografts to radiotherapy.
abstract: Phosphoglycerate kinase 1 (PGK1) has been demonstrated to be involved in radioresistance. The present study was designed to investigate the effect of PGK1 on the radioresistance in vivo. U251 glioma cells were transfected with the short hairpin RNA (shRNA)-PGK1 and pcDNA3.1-PGK1 using Lipofectamine 2000. The radiosensitivity of U251 xenografts was observed by tumor growth curve following radiotherapy. Quantitative PCR, western blot analysis and immunohistochemistry were performed to evaluate PGK1 expression in the xenografts from the different tumor models. The expression of PGK1 was maximally inhibited in response to shRNA4 at 24 h after the transfection in vitro. Tumor growth of the U251 xenografts was significantly inhibited following treatment with shRNA-PGK1 and radiotherapy. The expression of PGK1 in vivo at the mRNA and protein levels was downregulated by the treatment of shRNA1 when compared to levels following treatment with shNC and PBS after radiotherapy. The results showed that suppression of PGK1 enhanced the radiosensitivity of U251 xenografts and suggest that PGK1 may serve as a useful target in the treatment of radioresistant glioma.
authors.affiliation: Department of Neurosurgery, Nanjing Brain Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, P.R. China.
authors.forename: Yi-Jun
authors.initials: YJ
authors.lastname: Cheng
authors.affiliation: Department of Neurosurgery, Nanjing Brain Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Ding
authors.affiliation: Neuro-Psychiatric Institute, Nanjing Brain Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, P.R. China.
authors.forename: Hua-Qing
authors.initials: HQ
authors.lastname: Du
authors.affiliation: Department of Neurosurgery, Nanjing Brain Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, P.R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yan
authors.affiliation: Department of Neurosurgery, Nanjing Brain Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, P.R. China.
authors.forename: Jin-Bing
authors.initials: JB
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, Nanjing Brain Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, P.R. China.
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Nanjing Brain Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, P.R. China.
authors.forename: Yuan-Jie
authors.initials: YJ
authors.lastname: Zou
authors.affiliation: Department of Neurosurgery, Nanjing Brain Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, P.R. China.
authors.forename: Hong-Yi
authors.initials: HY
authors.lastname: Liu
authors.affiliation: Neuro-Psychiatric Institute, Nanjing Brain Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xiao
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D015536:Down-Regulation; D055785:Gene Knockdown Techniques; D005910:Glioma / Q000235:genetics* / Q000532:radiotherapy; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D034741:RNA, Small Interfering; D011827:Radiation; D011836:Radiation Tolerance / Q000235:genetics*; D023041:Xenograft Model Antitumor Assays
chemical_list: D034741:RNA, Small Interfering; C577282:PGK1 protein, human; D010735:Phosphoglycerate Kinase