title: Noncovalent dimer formation in liquid chromatography-mass spectrometry analysis.
abstract: Evidence will be presented that in the article "Novel LC-MS(2) Product Dependent Parallel Data Acquisition Function and Data Analysis Workflow for Sequencing and Identification of Intact Glycopeptides" written by Wu, S.-W.; Pu, T.-H.; Viner, R.; Khoo, K.-H. in Anal. Chem. 2014, 86, 5478-5486, noncovalent homo- and heterodimers were mis-identified as glycopeptides bearing well-defined N-linked structures, where the unexplained mass was attributed to excessive O-glycosylation. Noncovalent dimer formation of abundant components has not previously been considered as a complication in high-throughput proteomic analyses.
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, University of California San Francisco , 600 16th Street Genentech Hall, N474A, Box 2240, San Francisco, California 94158-2517, United States.
authors.forename: Katalin F
authors.initials: KF
authors.lastname: Medzihradszky
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006020:Glycopeptides / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D006020:Glycopeptides