title: GLP-1 receptor agonist increases the expression of CTRP3, a novel adipokine, in 3T3-L1 adipocytes through PKA signal pathway.
abstract: OBJECTIVE
  To investigate the effects of Exendin-4 (Ex-4), a glucagon-like peptide-1 (GLP-1) receptor agonist, on the expression of C1q/TNF-related protein-3 (CTRP3), a novel adipokine, in 3T3-L1 adipocytes. The role of protein kinase A (PKA) signal pathway in the effects was also investigated.
  
  
  METHODS
  The mRNA and protein expressions of CTRP3 in 3T3-L1 adiocytes were detected by real-time polymerase chain reaction and western blot, respectively. Exendin-fragment 9-39 (Ex-9), a specific GLP-1 receptor antagonist, and H89, a selective antagonist of PKA, were used to confirm the signal pathway of Ex-4 on CTRP3.
  
  
  RESULTS
  2.5 or 5.0 nmol/l Ex-4 treatment for 8 h increased the expressions of CTRP3 mRNA and protein as well as PKA protein in 3T3-L1 adipocytes significantly, while Ex-9 or H89 blocked the up-regulation of CTRP3 expression induced by Ex-4 completely.
  
  
  CONCLUSIONS
  GLP-1 receptor agonist increases the expression of CTRP3 mRNA and protein in 3T3-L1 adipocytes via PKA signal pathway.
authors.affiliation: Department of Endocrinology, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China. wdy2003win@163.com.
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Internal Medicine, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China.
authors.forename: L
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Endocrinology, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China.
authors.forename: M
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Endocrinology, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Endocrinology, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China.
authors.forename: S
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of Endocrinology, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China.
authors.forename: J
authors.initials: J
authors.lastname: Sun
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D054392:Adipokines / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D000077270:Exenatide; D005786:Gene Expression Regulation; D052216:Glucagon-Like Peptide 1 / Q000819:agonists* / Q000378:metabolism; D051379:Mice; D010455:Peptides / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D014688:Venoms / Q000494:pharmacology*
chemical_list: D054392:Adipokines; C428135:CORS26 protein, mouse; D010455:Peptides; D014688:Venoms; D052216:Glucagon-Like Peptide 1; D000077270:Exenatide; D017868:Cyclic AMP-Dependent Protein Kinases
keywords: C1q/TNF-related protein-3 (CTRP3); Glucagon-like peptide-1 (GLP-1); Protein kinase A (PKA)