title: Progesterone receptor expression in granulosa cells is suppressed by microRNA-378-3p.
abstract: In developing ovarian follicles, the progesterone receptor (PGR) is essential for mediating transcription of key factors that coordinate cellular functions including follicular remodeling. With recent investigations examining the role of microRNA (miRNA) in regulating ovarian function we used a lentiviral approach to over express miR-378 in cultured primary porcine granulosa cells to study the role this miRNA may play in granulosa cell development. We revealed that miR-378-3p decreased protein levels and mRNA levels of PGR via targeting its 3'UTR. We observed that this regulation of PGR by miR-378-3p resulted in a corresponding decrease in gene transcripts of ADAMTS1, CTSL1, and PPARG, all known to be regulated by PGR and important for follicular maturation and remodeling. Our study provides the first evidence for post-transcriptional regulation of PGR and further elucidates the role of miR-378-3p in the ovary.
authors.affiliation: Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
authors.forename: Derek
authors.initials: D
authors.lastname: Toms
authors.affiliation: Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1; Institute of Animal Nutrition, Sichuan Agricultural University, 625014, China.
authors.forename: Shengyu
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
authors.forename: Bo
authors.initials: B
authors.lastname: Pan
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, 625014, China. Electronic address: pig2pig@sina.com.
authors.forename: De
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1. Electronic address: jli@uoguelph.ca.
authors.forename: Julang
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006107:Granulosa Cells / Q000166:cytology / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000096:biosynthesis* / Q000235:genetics; D013552:Swine
chemical_list: D035683:MicroRNAs; D011980:Receptors, Progesterone
keywords: Granulosa cell; MicroRNA; Ovary; Progesterone receptor; miR-378