title: The central domain of yeast transcription factor Rpn4 facilitates degradation of reporter protein in human cells.
abstract: Despite high interest in the cellular degradation machinery and protein degradation signals (degrons), few degrons with universal activity along species have been identified. It has been shown that fusion of a target protein with a degradation signal from mammalian ornithine decarboxylase (ODC) induces fast proteasomal degradation of the chimera in both mammalian and yeast cells. However, no degrons from yeast-encoded proteins capable to function in mammalian cells were identified so far. Here, we demonstrate that the yeast transcription factor Rpn4 undergoes fast proteasomal degradation and its central domain can destabilize green fluorescent protein and Alpha-fetoprotein in human HEK 293T cells. Furthermore, we confirm the activity of this degron in yeast. Thus, the Rpn4 central domain is an effective interspecies degradation signal.
authors.affiliation: W.A. Engelhardt Institute of Molecular Biology RAS, Moscow, Russia. Electronic address: Runkel@inbox.ru.
authors.forename: A V
authors.initials: AV
authors.lastname: Morozov
authors.affiliation: W.A. Engelhardt Institute of Molecular Biology RAS, Moscow, Russia.
authors.forename: D S
authors.initials: DS
authors.lastname: Spasskaya
authors.affiliation: W.A. Engelhardt Institute of Molecular Biology RAS, Moscow, Russia.
authors.forename: D S
authors.initials: DS
authors.lastname: Karpov
authors.affiliation: W.A. Engelhardt Institute of Molecular Biology RAS, Moscow, Russia.
authors.forename: V L
authors.initials: VL
authors.lastname: Karpov
mesh_terms: D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D017434:Protein Structure, Tertiary; D059748:Proteolysis; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000509:alpha-Fetoproteins / Q000378:metabolism*
chemical_list: C097353:AFP protein, human; D004268:DNA-Binding Proteins; C119430:RPN4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000509:alpha-Fetoproteins; D046988:Proteasome Endopeptidase Complex
keywords: Proteasome; Protein degradation signals; Rpn4