title: [Levels of complement components C3a and C5a in renal injury among trichloroethylene-sensitized BALB/c mice].
abstract: OBJECTIVE
  To determine the levels of complement components C3a and C5a in the kidneys of trichloroethylene (TCE)-sensitized BALB/c mice, and to investigate the role of complement components in TCE-induced renal injury among BALB/c mice.
  
  
  METHODS
  Sixty-two female BALB/c mice were randomly divided into blank control group, vehicle control group, and TCE sensitization group. The mice in TCE sensitization group were sensitized by one intracutaneous injection and one abdominal smear of TCE. At 24 h, 48 h, 72 h, and 7 d after the second sensitization, mice were sacrificed, and the blood and kidneys were collected. An automatic biochemical analyzer was used in the determination of serum blood urea nitrogen (BUN) and creatinine (Cr). The levels of C3a and C5a in the kidneys were determined by immunohistochemistry.
  
  
  RESULTS
  The sensitization rate of TCE sensitization group was 42.0%. Kidney coefficient and serum levels of BUN and Cr were significantly increased in the TCE sensitization group as compared with the vehicle control group at 48 h and 72 h after sensitization (P < 0.05). The kidney coefficients of the TCE sensitization group at 48 h and 72 h were significantly higher than those of the control groups (P < 0.05). In comparison with the vehicle control group, however, no significant change was found in kidney coefficient, serum BUN, or serum Cr at 7 d after TCE sensitization (P > 0.05). Levels of C3a and C5a at 48 h (3.80±0.84 and 4.00±1.00, respectively) and 72 h (4.40 ± 1.14 and 4.40 ± 1.14, respectively) after sensitization were all significantly higher than those of the vehicle control group (P < 0.05), but no significant difference was found in level of C3a (1.80±0.45) or C5a (2.00 ± 0.71) at 7 d (P > 0.05).
  
  
  CONCLUSIONS
  TCE sensitization can induce renal injury in mice. Levels of complement components C3a and C5a are elevated in the kidneys of sensitized mice, indicating that C3a and C5a may be involved in the renal injury induced by TCE sensitization.
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Anhui Medical University, Hefei 230032, China.
authors.forename: Wansheng
authors.initials: W
authors.lastname: Zha
authors.forename: Jing
authors.initials: J
authors.lastname: Leng
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Jiaxiang
authors.initials: J
authors.lastname: Zhang
authors.forename: Shulong
authors.initials: S
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Tong
authors.initials: T
authors.lastname: Shen
authors.affiliation: E-mail: zqxing@yeah.net.
authors.forename: Qixing
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001806:Blood Urea Nitrogen; D015926:Complement C3a / Q000378:metabolism*; D015936:Complement C5a / Q000378:metabolism*; D003404:Creatinine / Q000097:blood; D005260:Female; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014241:Trichloroethylene / Q000633:toxicity*
chemical_list: D014241:Trichloroethylene; D015926:Complement C3a; D015936:Complement C5a; D003404:Creatinine