title: Immobilization of His-tagged kinase JAK2 onto the surface of a plasmon resonance gold disc modified with different copper (II) complexes.
abstract: New surface plasmon resonance (SPR) sensing platforms which consists of copper (II) complexes of a pentetic acid thiol ligand (DPTA-Cu(II)) and of a thiol derivative of dipyrromethene (DPM-Cu(II) created on the surface of gold SPR disc were applied to oriented immobilization of His-tagged Janus kinase 2 (GST-His6-JAK2). This method is based on the covalent bond formation between histidine from a His-tag chain of a protein and Cu(II) centres from the complexes. The kinetic and thermodynamic parameters of the oriented immobilization of GST-His6-JAK2 protein to DPTA-Cu(II) and DPM-Cu(II) complexes attached to the Au surface of a SPR disc were discussed.
authors.affiliation: Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10 Street, 10-748 Olsztyn, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kurzątkowska
authors.affiliation: Institute of Biochemistry and Biophysics of the Polish Academy of Sciences, Pawińskiego 5A Street, 02-106 Warsaw, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Mielecki
authors.affiliation: Institute of Biochemistry and Biophysics of the Polish Academy of Sciences, Pawińskiego 5A Street, 02-106 Warsaw, Poland.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Grzelak
authors.affiliation: University of Leuven, Chemistry Department, Celestijnenlaan 200F, B-3001 Leuven, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Verwilst
authors.affiliation: University of Leuven, Chemistry Department, Celestijnenlaan 200F, B-3001 Leuven, Belgium.
authors.forename: Wim
authors.initials: W
authors.lastname: Dehaen
authors.affiliation: Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10 Street, 10-748 Olsztyn, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Radecki
authors.affiliation: Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10 Street, 10-748 Olsztyn, Poland. Electronic address: h.radecka@pan.olsztyn.pl.
authors.forename: Hanna
authors.initials: H
authors.lastname: Radecka
mesh_terms: D015374:Biosensing Techniques; D056831:Coordination Complexes / Q000737:chemistry* / Q000378:metabolism; D003300:Copper / Q000737:chemistry* / Q000378:metabolism; D003911:Dextrans / Q000737:chemistry; D006046:Gold / Q000737:chemistry* / Q000378:metabolism; D006639:Histidine / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D053614:Janus Kinase 2 / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D013438:Sulfhydryl Compounds / Q000737:chemistry* / Q000378:metabolism; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D013816:Thermodynamics
chemical_list: D056831:Coordination Complexes; D003911:Dextrans; D013438:Sulfhydryl Compounds; D006639:Histidine; D006046:Gold; D003300:Copper; C507924:JAK2 protein, human; D053614:Janus Kinase 2
keywords: Dipyrromethene-Cu(II); His-tagged protein immobilization; Janus Kinase 2; Kinetic and thermodynamic analysis; Pentetic acid thiol ligand-Cu(II); Surface plasmon resonance biosensors