title: Asymmetric mRNA localization contributes to fidelity and sensitivity of spatially localized systems.
abstract: Although many proteins are localized after translation, asymmetric protein distribution is also achieved by translation after mRNA localization. Why are certain mRNA transported to a distal location and translated on-site? Here we undertake a systematic, genome-scale study of asymmetrically distributed protein and mRNA in mammalian cells. Our findings suggest that asymmetric protein distribution by mRNA localization enhances interaction fidelity and signaling sensitivity. Proteins synthesized at distal locations frequently contain intrinsically disordered segments. These regions are generally rich in assembly-promoting modules and are often regulated by post-translational modifications. Such proteins are tightly regulated but display distinct temporal dynamics upon stimulation with growth factors. Thus, proteins synthesized on-site may rapidly alter proteome composition and act as dynamically regulated scaffolds to promote the formation of reversible cellular assemblies. Our observations are consistent across multiple mammalian species, cell types and developmental stages, suggesting that localized translation is a recurring feature of cell signaling and regulation.
authors.affiliation: 1] MRC Laboratory of Molecular Biology, Cambridge, UK. [2] Present address: The Donnelly Centre, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Weatheritt
authors.affiliation: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Toby J
authors.initials: TJ
authors.lastname: Gibson
authors.affiliation: MRC Laboratory of Molecular Biology, Cambridge, UK.
authors.forename: M Madan
authors.initials: MM
authors.lastname: Babu
mesh_terms: D000818:Animals; D051379:Mice; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D034443:RNA Transport; D012333:RNA, Messenger / Q000032:analysis* / Q000378:metabolism*; D051381:Rats
chemical_list: D011506:Proteins; D012333:RNA, Messenger