title: Atlantic salmon possesses two clusters of type I interferon receptor genes on different chromosomes, which allows for a larger repertoire of interferon receptors than in zebrafish and mammals.
abstract: Mammalian type I interferons (IFNs) signal through a receptor composed of the IFNAR1 and IFNAR2 chains. In zebrafish two-cysteine IFNs utilize a receptor composed of CRFB1 and CRFB5, while four-cysteine IFNs signal through a receptor formed by CRFB2 and CRFB5. In the present work two CRFB clusters were identified in different chromosomes of Atlantic salmon. Genes of three CRFB5s, one CRFB1, one CRFB2 and the novel CRFB5x were identified, cloned and studied functionally. All CRFBs were expressed in 10 different organs, but the relative expression of CRFBs varied. Mx-reporter assay was used to study which CRFBs might be involved in receptors for salmon IFNa, IFNb and IFNc. The results of Mx-reporter assays suggest that IFNa signals through a receptor composed of CRFB1a as the long chain and either CRFB5a, CRFB5b or CRFB5c as the short chain; IFNc signals through a receptor with CRFB5a or CRFB5c as the short chain while IFNb may signal through a receptor with CRFB5x as a short chain. Taken together, the present work demonstrates that Atlantic salmon has a more diverse repertoire of type I IFN receptors compared to zebrafish or mammals.
authors.affiliation: Norwegian College of Fishery Science, University of Tromsø, 9037 Tromsø, Norway.
authors.forename: Baojian
authors.initials: B
authors.lastname: Sun
authors.affiliation: Norwegian College of Fishery Science, University of Tromsø, 9037 Tromsø, Norway.
authors.forename: Linn
authors.initials: L
authors.lastname: Greiner-Tollersrud
authors.affiliation: Centre for Biomedical Research, Department of Biology, University of Victoria, PO Box 3020 STN CSC, Victoria, Canada.
authors.forename: Ben F
authors.initials: BF
authors.lastname: Koop
authors.affiliation: Norwegian College of Fishery Science, University of Tromsø, 9037 Tromsø, Norway. Electronic address: borre.robertsen@uit.no.
authors.forename: Børre
authors.initials: B
authors.lastname: Robertsen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002875:Chromosomes; D003001:Cloning, Molecular; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D016898:Interferon-alpha / Q000235:genetics / Q000276:immunology*; D016899:Interferon-beta / Q000235:genetics / Q000276:immunology*; D007668:Kidney / Q000166:cytology / Q000276:immunology; D007962:Leukocytes / Q000166:cytology / Q000276:immunology; D008156:Luciferases / Q000235:genetics / Q000276:immunology; D008322:Mammals / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D005810:Multigene Family; D009928:Organ Specificity; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics / Q000276:immunology; D017471:Receptors, Interferon / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D020317:Salmo salar / Q000145:classification / Q000235:genetics / Q000276:immunology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013045:Species Specificity; D015027:Zebrafish / Q000235:genetics / Q000276:immunology; D000096743:Interferon gamma Receptor
chemical_list: D029941:Fish Proteins; D016898:Interferon-alpha; D016899:Interferon-beta; D008156:Luciferases; D020033:Protein Isoforms; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D000096743:Interferon gamma Receptor
keywords: Atlantic salmon; CRFB; Fish; IFN; Interferon; Receptor