title: Synthesis and characterization of amino acid conjugates of oleanolic acid and their in vitro cytotoxic effect on HCC cell lines.
abstract: Oleanolic acid (3β-hydroxy-olean-12-en-28-oic acid; OA-01), a pentacyclic triterpene, exhibit a wide range of pharmacological and biological activities. We have isolated oleanolic acid from methanolic extract of Periploca aphylla, collected from surroundings of Karachi in the month of February. Furthermore, four known and two new C-28 amino acid conjugates of oleanolic acid were prepared to explore potential of these compounds on HCCs and one breast cancer cell line. Cytotoxic effects revealed that as compare to parent compound (OA-01), two derivatives OA-04 (p<0.0001) and OA-06 (p<0.01) showed significantly increased/higher inhibition rates.
authors.affiliation: Bilkent University, BilGen Genetics and Biotechnology Research Center, Deptt of Molecular Biology and Genetics, Ankara, Turkey / BIPS and Department of Biochemistry, Baqai Medical University, Karachi, Pakistan.
authors.forename: Muhammad Ayaz
authors.initials: MA
authors.lastname: Mustufa
authors.affiliation: Department of Chemistry, University of Karachi, Karachi, Pakistan.
authors.forename: Imran Ali
authors.initials: IA
authors.lastname: Hashmi
authors.affiliation: Department of Chemistry, University of Karachi, Karachi, Pakistan.
authors.forename: Shahbaz
authors.initials: S
authors.lastname: Manzoor
authors.affiliation: Pakistan Council of Scientific and Industrial Research, Karachi, Pakistan.
authors.forename: Aamir
authors.initials: A
authors.lastname: Ahmed
authors.affiliation: HEJ Research Institute of Chemistry, University of Karachi, Karachi, Pakistan.
authors.forename: Viqar-ud-din
authors.initials: VU
authors.lastname: Ahmed
authors.affiliation: Pakistan Council of Scientific and Industrial Research, Karachi, Pakistan.
authors.forename: Afshan
authors.initials: A
authors.lastname: Aslam
authors.affiliation: Bilkent University, BilGen Genetics and Biotechnology Research Center, Deptt of Molecular Biology and Genetics, Ankara, Turkey.
authors.forename: Cigdem
authors.initials: C
authors.lastname: Ozen
authors.affiliation: BIPS and Department of Biochemistry, Baqai Medical University, Karachi, Pakistan.
authors.forename: Naim ul Hasan
authors.initials: Nu
authors.lastname: Naqvi
authors.affiliation: Bilkent University, BilGen Genetics and Biotechnology Research Center, Deptt of Molecular Biology and Genetics, Ankara, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ozturk
authors.affiliation: Department of Chemistry, University of Karachi, Karachi, Pakistan.
authors.forename: Firdous Imran
authors.initials: FI
authors.lastname: Ali
mesh_terms: D000596:Amino Acids / Q000138:chemical synthesis* / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000138:chemical synthesis* / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D008113:Liver Neoplasms / Q000473:pathology*; D061986:MCF-7 Cells; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000494:pharmacology*; D031109:Periploca; D008517:Phytotherapy; D024301:Plant Bark; D018515:Plant Leaves; D010946:Plants, Medicinal
chemical_list: D000596:Amino Acids; D000972:Antineoplastic Agents, Phytogenic; D009828:Oleanolic Acid