title: Early lineage restriction in temporally distinct populations of Mesp1 progenitors during mammalian heart development.
abstract: Cardiac development arises from two sources of mesoderm progenitors, the first heart field (FHF) and the second (SHF). Mesp1 has been proposed to mark the most primitive multipotent cardiac progenitors common for both heart fields. Here, using clonal analysis of the earliest prospective cardiovascular progenitors in a temporally controlled manner during early gastrulation, we found that Mesp1 progenitors consist of two temporally distinct pools of progenitors restricted to either the FHF or the SHF. FHF progenitors were unipotent, whereas SHF progenitors were either unipotent or bipotent. Microarray and single-cell PCR with reverse transcription analysis of Mesp1 progenitors revealed the existence of molecularly distinct populations of Mesp1 progenitors, consistent with their lineage and regional contribution. Together, these results provide evidence that heart development arises from distinct populations of unipotent and bipotent cardiac progenitors that independently express Mesp1 at different time points during their specification, revealing that the regional segregation and lineage restriction of cardiac progenitors occur very early during gastrulation.
authors.affiliation: 1] Université Libre de Bruxelles, IRIBHM, Brussels B-1070, Belgium [2].
authors.forename: Fabienne
authors.initials: F
authors.lastname: Lescroart
authors.affiliation: 1] Université Libre de Bruxelles, IRIBHM, Brussels B-1070, Belgium [2].
authors.forename: Samira
authors.initials: S
authors.lastname: Chabab
authors.affiliation: Université Libre de Bruxelles, IRIBHM, Brussels B-1070, Belgium.
authors.forename: Xionghui
authors.initials: X
authors.lastname: Lin
authors.affiliation: 1] Cavendish Laboratory, Department of Physics, J. J. Thomson Avenue, Cambridge CB3 0HE, UK [2] The Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK.
authors.forename: Steffen
authors.initials: S
authors.lastname: Rulands
authors.affiliation: Université Libre de Bruxelles, IRIBHM, Brussels B-1070, Belgium.
authors.forename: Catherine
authors.initials: C
authors.lastname: Paulissen
authors.affiliation: Functional Genomics Core, Institute for Research in Biomedicine, Barcelona 08028, Spain.
authors.forename: Annie
authors.initials: A
authors.lastname: Rodolosse
authors.affiliation: Functional Genomics Core, Institute for Research in Biomedicine, Barcelona 08028, Spain.
authors.forename: Herbert
authors.initials: H
authors.lastname: Auer
authors.affiliation: Université Catholique de Louvain, de Duve Institute, Brussels B-1200, Belgium.
authors.forename: Younes
authors.initials: Y
authors.lastname: Achouri
authors.affiliation: Université Libre de Bruxelles, IRIBHM, Brussels B-1070, Belgium.
authors.forename: Christine
authors.initials: C
authors.lastname: Dubois
authors.affiliation: 1] Université Libre de Bruxelles, IRIBHM, Brussels B-1070, Belgium [2] Department of Cardiology, Hopital Erasme, Brussels B-1070, Belgium.
authors.forename: Antoine
authors.initials: A
authors.lastname: Bondue
authors.affiliation: 1] Cavendish Laboratory, Department of Physics, J. J. Thomson Avenue, Cambridge CB3 0HE, UK [2] The Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK.
authors.forename: Benjamin D
authors.initials: BD
authors.lastname: Simons
authors.affiliation: 1] Université Libre de Bruxelles, IRIBHM, Brussels B-1070, Belgium [2] WELBIO, Université Libre de Bruxelles, Brussels B-1070, Belgium.
authors.forename: Cédric
authors.initials: C
authors.lastname: Blanpain
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D019521:Body Patterning; D002454:Cell Differentiation; D019070:Cell Lineage; D005260:Female; D005318:Fetal Heart / Q000166:cytology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006325:Heart Atria / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D006352:Heart Ventricles / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009928:Organ Specificity; D013234:Stem Cells / Q000502:physiology*; D059467:Transcriptome
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; C101538:Mesp1 protein, mouse