title: Evaluation of aBurkholderia pseudomalleiOuter Membrane Vesicle Vaccine in Nonhuman Primates.
abstract: Burkholderia pseudomallei(Bps)is the causative agent of melioidosis and is endemic in regions of northern Australia and Southeast Asia.Bpsis inherently resistant to multiple antibiotics and is considered a potential biological warfare agent by the U.S. DHHS. Therefore, effective vaccines are necessary to prevent natural infection and to safeguard against biological attack with this organism. In our previous work we have shown that immunization with naturally derived outer membrane vesicles (OMVs) fromBpsprovides significant protection against lethal aerosol and systemic infection in BALB/c mice. In this work, we evaluated the safety and immunogenicity of escalating doses of OMV vaccine in rhesus macaques. We show that immunization of rhesus macaques withBpsOMVs generates humoral immuneresponses to protective protein and polysaccharide antigens without any associated toxicity or reactogenicity. These results lay the groundwork for evaluation of protective efficacy of the OMV vaccine in the nonhuman primate model of melioidosis.
authors.affiliation: Tulane University School of Medicine, New Orleans LA 70112, USA.
authors.forename: Hailey
authors.initials: H
authors.lastname: Petersen
authors.affiliation: Tulane University School of Medicine, New Orleans LA 70112, USA.
authors.forename: Wildaliz
authors.initials: W
authors.lastname: Nieves
authors.affiliation: Tulane National Primate Research Center, Covington LA 70433, USA.
authors.forename: Kasi
authors.initials: K
authors.lastname: Russell-Lodrigue
authors.affiliation: Tulane University School of Medicine, New Orleans LA 70112, USA ; Tulane National Primate Research Center, Covington LA 70433, USA.
authors.forename: Chad J
authors.initials: CJ
authors.lastname: Roy
authors.affiliation: Tulane University School of Medicine, New Orleans LA 70112, USA.
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Morici
keywords: Burkholderia pseudomallei; Outer membrane vesicle; melioidosis; primate; vaccine