title: [Changes of proliferation and angiogenesis of residual tumor in rabbit lung after radiofrequency ablation].
abstract: OBJECTIVE
  To observe the changes of proliferation and angiogenesis of residual tumor in rabbit lung after radiofrequency ablation (RFA).
  
  
  METHODS
  The model of VX2 tumor in rabbit lung was established by injection of tissue block suspension. 64 New Zealand White rabbits bearing VX2 tumor were assigned randomly to the control group (n = 10) and the RFA group (n = 48). During the RFA procedure, residual tumors were achieved by controlling the range of electrode expanding, output power and treatment time. At several points of time, Ki-67 labeling index (Ki-67LI) and microvessel density (MVD) of the residual tumors were calculated by immunohistochemical detection.
  
  
  RESULTS
  Ki-67LI of the control group was 45.3% ± 2.1%. Ki-67LI of the RFA group at the first, 3 and 5 day were 56.4% ± 3.4%, 60.1% ± 4.1% and 59.8% ± 2.4% respectively, significantly higher than that of the control group; however, at the seventh, 9, 14 and 21 day, they were 45.4% ± 2.0%, 46.2% ± 3.4%, 45.1% ± 4.4% and 47.8% ± 3.9% respectively, no significant difference compared with the control group. The control group MVD was 28.9 ± 2.9. MVD of the RFA group at third, 5 and 7 day were 36.8 ± 2.6, 55.6 ± 4.8 and 51.5 ± 2.8 respectively, significantly higher than that of the control group; however, at the first, 9, 14 and 21 day were 27 ± 2.8, 29.2 ± 3.2, 30 ± 2.8 and 28.8 ± 3.1 respectively, no significant difference compared with the control group.
  
  
  CONCLUSIONS
  The proliferation and angiogenesis of pulmonary residual tumor exhibit a transient increase phenomenon after RFA.
authors.affiliation: Department of Thoracic Surgery, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Lianjun
authors.initials: L
authors.lastname: Ma
authors.affiliation: Email: znkljp@sina.com.
authors.forename: Naikang
authors.initials: N
authors.lastname: Zhou
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Qi
authors.forename: Yanghong
authors.initials: Y
authors.lastname: Peng
authors.forename: Shuxin
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017115:Catheter Ablation; D049109:Cell Proliferation; D004566:Electrodes; D008175:Lung Neoplasms / Q000098:blood supply* / Q000473:pathology*; D018365:Neoplasm, Residual; D009389:Neovascularization, Pathologic; D011817:Rabbits