title: Acquisition and role of molybdate in Pseudomonas aeruginosa.
abstract: In microaerophilic or anaerobic environments, Pseudomonas aeruginosa utilizes nitrate reduction for energy production, a process dependent on the availability of the oxyanionic form of molybdenum, molybdate (MoO4 (2-)). Here, we show that molybdate acquisition in P. aeruginosa occurs via a high-affinity ATP-binding cassette permease (ModABC). ModA is a cluster D-III solute binding protein capable of interacting with molybdate or tungstate oxyanions. Deletion of the modA gene reduces cellular molybdate concentrations and results in inhibition of anaerobic growth and nitrate reduction. Further, we show that conditions that permit nitrate reduction also cause inhibition of biofilm formation and an alteration in fatty acid composition of P. aeruginosa. Collectively, these data highlight the importance of molybdate for anaerobic growth of P. aeruginosa and reveal novel consequences of nitrate reduction on biofilm formation and cell membrane composition.
authors.affiliation: Research Centre for Infectious Diseases, School of Molecular and Biomedical Sciences, University of Adelaide, Adelaide, South Australia, Australia.
authors.forename: Victoria G
authors.initials: VG
authors.lastname: Pederick
authors.affiliation: Research Centre for Infectious Diseases, School of Molecular and Biomedical Sciences, University of Adelaide, Adelaide, South Australia, Australia.
authors.forename: Bart A
authors.initials: BA
authors.lastname: Eijkelkamp
authors.affiliation: Research Centre for Infectious Diseases, School of Molecular and Biomedical Sciences, University of Adelaide, Adelaide, South Australia, Australia.
authors.forename: Miranda P
authors.initials: MP
authors.lastname: Ween
authors.affiliation: Research Centre for Infectious Diseases, School of Molecular and Biomedical Sciences, University of Adelaide, Adelaide, South Australia, Australia.
authors.forename: Stephanie L
authors.initials: SL
authors.lastname: Begg
authors.affiliation: Research Centre for Infectious Diseases, School of Molecular and Biomedical Sciences, University of Adelaide, Adelaide, South Australia, Australia.
authors.forename: James C
authors.initials: JC
authors.lastname: Paton
authors.affiliation: Research Centre for Infectious Diseases, School of Molecular and Biomedical Sciences, University of Adelaide, Adelaide, South Australia, Australia christopher.mcdevitt@adelaide.edu.au.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: McDevitt
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000693:Anaerobiosis; D005227:Fatty Acids / Q000032:analysis; D017353:Gene Deletion; D008982:Molybdenum / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism; D010084:Oxidation-Reduction; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D005227:Fatty Acids; D009566:Nitrates; C044659:molybdate; D008982:Molybdenum