title: Attenuation of lung cancer stem cell tumorigenesis and metastasis by cisplatin.
abstract: OBJECTIVE
  To investigate the effect of cisplatin on the growth and metastasis abilities of lung cancer stem cells (CSCs) via molecular imaging.
  
  
  METHODS
  The expression changes of lung CSCs cell marker in A549-Luc-C8 human non-small-cell lung cancer (NSCLC) cell line with or without cisplatin treatment were detected by flow cytometry. The tumorigenesis and metastasis abilities of A549-Luc-C8 cells were monitored both in vitro and in vivo, and the mechanism was assessed by gene sequencing.
  
  
  RESULTS
  About 1%-2% of CSCs were detected in A549-Luc-C8 cells and decreased CSCs percentage was observed after cisplatin treatment. Attenuated tumorigenesis and metastasis abilities of A549-Luc-C8 cells were found in cisplatin treated group.
  
  
  CONCLUSIONS
  Decreased percentage of CSCs in A549-Luc-C8 cells can be induced by cisplatin treatment, which may partly be attributed to the attenuated expression of growth factors.
authors.affiliation: Cardiology Department, Chinese PLA General Hospital, Beijing, China.|Cardiology Department, No. 150 Hospital of PLA, LuoYang, Henan, China.
authors.forename: Shenxu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Cardiology Department, Xijing Hospital, Xi'an, Shaanxi, China.
authors.forename: Sai
authors.initials: S
authors.lastname: Ma
authors.affiliation: Cardiology Department, Xijing Hospital, Xi'an, Shaanxi, China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Li
authors.affiliation: Digestive Disease Department, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Zengfu
authors.initials: Z
authors.lastname: Xue
authors.affiliation: Cardiology Department, Xijing Hospital, Xi'an, Shaanxi, China.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Cardiology Department, Xijing Hospital, Xi'an, Shaanxi, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Fan
authors.affiliation: Digestive Disease Department, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Yongzhan
authors.initials: Y
authors.lastname: Nie
authors.affiliation: Digestive Disease Department, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Kaichun
authors.initials: K
authors.lastname: Wu
authors.affiliation: Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Chen
authors.affiliation: Cardiology Department, Chinese PLA General Hospital, Beijing, China.|Cardiology Department, Xijing Hospital, Xi'an, Shaanxi, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Cao
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D063646:Carcinogenesis / Q000187:drug effects; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy*; D008819:Mice, Nude; D014411:Neoplastic Stem Cells / Q000187:drug effects*; D017423:Sequence Analysis, RNA
chemical_list: D000970:Antineoplastic Agents; D002945:Cisplatin
keywords: cancer stem cells; cisplatin; growth factor; lung cancer; molecular imaging