title: Fractionation and identification of minor and aroma-active constituents in Kangra orthodox black tea.
abstract: The aroma constituents of Kangra orthodox black tea were isolated by simultaneous distillation extraction (SDE), supercritical fluid extraction and beverage method. The aroma-active compounds were identified using gas chromatography-olfactometry-mass spectrometry. Geraniol, linalool, (Z/E)-linalool oxides, (E)-2-hexenal, phytol, β-ionone, hotrienol, methylpyrazine and methyl salicylate were major volatile constituents in all the extracts. Minor volatile compounds in all the extracts were 2-ethyl-5-methylpyrazine, ethylpyrazine, 2-6,10,14-trimethyl-2-pentadecanone, acetylfuran, hexanoic acid, dihydroactinidiolide and (E/Z)-2,6-nonadienal. The concentrated SDE extract was fractionated into acidic, basic, water-soluble and neutral fractions. The neutral fraction was further chromatographed on a packed silica gel column eluted with pentane and diethyl ether to separate minor compounds. The aroma-active compounds identified using gas chromatography-olfactometry-mass spectrometry were 2-amylfuran, (E/Z)-2,6-nonadienal, 1-pentanol, epoxylinalool, (Z)-jasmone, 2-acetylpyrrole, farnesyl acetone, geranyl acetone, cadinol, cubenol and dihydroactinidiolide. AEDA studies showed 2-hexenal, 3-hexenol, ethylpyrazine, (Z/E)-linalool oxides, linalool, (E/Z)-2,6-nonadienal, geraniol, phenylethanol, β-ionone, hotrienol and dihydroactinidiolide to be odour active components.
authors.affiliation: Academy of Scientific and Innovative Research, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, India.
authors.forename: Robin
authors.initials: R
authors.lastname: Joshi
authors.affiliation: Academy of Scientific and Innovative Research, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, India. Electronic address: ashugulati@ihbt.res.in.
authors.forename: Ashu
authors.initials: A
authors.lastname: Gulati
mesh_terms: D005591:Chemical Fractionation; D009812:Odorants / Q000032:analysis*; D013662:Tea / Q000737:chemistry*
chemical_list: D013662:Tea
keywords: Extraction; Fractionation; Minor components; Odour impact; Orthodox black tea; Pyrazines