title: [Observing the effect of high glucose on proliferation of bone marrow stromal stem cells through Wnt/Β-catenin pathway].
abstract: OBJECTIVE
  To explore the effect of high glucose on proliferation of bone marrow stromal stem cells through Wnt/Β-catenin pathway.
  
  
  METHODS
  Bone marrow stormal cells were obtained from the mandible of Wistar rats and stimulated with different concentrations of glucose (5.5 and 16.5 mmol/L). Cell proliferation was evaluated with methyl thiazolyl tetrazolium assay (1, 3, 5, and 7 d)and cell cycle analysis by flow cytometry (5 d). Β-catenin and cyclin D1 protein levels were determined by Western blot. The mRNA expression of lymphoid enhancer binding factor-1 (LEF-1) and cyclin D1 were tested by real-time polymerase chain reaction.
  
  
  RESULTS
  The results of methyl thiazolyl tetrazolium assay indicated that the optical density values of two different concentrations of the glucose had no statistical difference on day 1 (P=0.700). On days 3, 5, and 7, the optical density values of the 16.5 mmol/L group were significantly lower than those in the 5.5 mmol/L group (P=0.006, P=0.002, and P=0.003). Cell cycle analysis indicated that high glucose concentration could reduced the progression from phase G1 to S, and the proliferation index values of the 16.5 mmol/L group were significantly lower than those of the 5.5 mmol/L group (P=0.014). The Β-catenin and cyclin D1 levels were lower in the 16.5 mmol/L group when compared with the 5.5 mmol/L group. High glucose condition also reduced the mRNA expressions of LEF-1 and cyclin D1.
  
  
  CONCLUSIONS
  High glucose can inhibit the proliferation of bone marrow stormal cells by suppressing the expressions of Β-catenin, LEF-1, and cyclin D1 in the Wnt/Β-catenin pathway.
authors.affiliation: Stomatological Center,Chinese PLA General Hospital,Beijing 100853,China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Stomatological Center,Chinese PLA General Hospital,Beijing 100853,China.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.affiliation: Stomatological Center,Chinese PLA General Hospital,Beijing 100853,China.
authors.forename: Bin
authors.initials: B
authors.lastname: Gu
authors.affiliation: Stomatological Center,Chinese PLA General Hospital,Beijing 100853,China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: Stomatological Center,Chinese PLA General Hospital,Beijing 100853,China.
authors.forename: Yu-xuan
authors.initials: YX
authors.lastname: Gao
authors.affiliation: Stomatological Center,Chinese PLA General Hospital,Beijing 100853,China.
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Wang
authors.affiliation: Stomatological Center,Chinese PLA General Hospital,Beijing 100853,China.
authors.forename: Hong-chen
authors.initials: HC
authors.lastname: Liu
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D049109:Cell Proliferation / Q000187:drug effects; D019938:Cyclin D1 / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000378:metabolism*; D008297:Male; D008334:Mandible / Q000166:cytology; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000378:metabolism*
chemical_list: C530106:Ccnd1 protein, rat; C497575:Lef1 protein, rat; D051821:Lymphoid Enhancer-Binding Factor 1; D051176:beta Catenin; D019938:Cyclin D1; D005947:Glucose