title: Is the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration?
abstract: The purpose of this study was to compare: a new five-layered poly (L-lactide-co-caprolactone) (PLC) membrane and small intestinal submucosa (SIS) as a control in rat urinary bladder wall regeneration. The five-layered poly (L-lactide-co-caprolactone) membrane was prepared by an electrospinning process. Adipose tissue was harvested from five 8-week old male Wistar rats. Adipose derived stem cells (ADSCs) were seeded in a density of 3×10(6) cells/cm2 onto PLC membrane and SIS scaffolds, and cultured for 5-7 days in the stem cell culture medium. Twenty male Wistar rats were randomly divided into five equal groups. Augmentation cystoplasty was performed in a previously created dome defect. Groups: (I) PLC+ 3×10(6)ADSCs; (II) SIS+ 3×10(6)ADSCs; (III) PLC; (IV) SIS; (V) control. Cystography was performed after three months. The reconstructed urinary bladders were evaluated in H&E and Masson's trichrome staining. Regeneration of all components of the normal urinary bladder wall was observed in bladders augmented with cell-seeded SIS matrices. The urinary bladders augmented with SIS matrices without cells showed fibrosis and graft contraction. Bladder augmentation with the PLC membrane led to numerous undesirable events including: bladder wall perforation, fistula or diverticula formation, and incorporation of the reconstructed wall into the bladder lumen. The new five-layered poly (L-lactide-co-caprolactone) membrane possesses poorer potential for regenerating the urinary bladder wall compared with SIS scaffold.
authors.affiliation: Chair of Regenerative Medicine, Department of Tissue Engineering, Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Bydgoszcz, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Pokrywczynska
authors.affiliation: Chair of Regenerative Medicine, Department of Tissue Engineering, Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Bydgoszcz, Poland.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Jundzill
authors.affiliation: Chair of Regenerative Medicine, Department of Tissue Engineering, Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Bydgoszcz, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Adamowicz
authors.affiliation: Department of Theory of Continuous Media, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Kowalczyk
authors.affiliation: Chair of Regenerative Medicine, Department of Tissue Engineering, Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Bydgoszcz, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Warda
authors.affiliation: Chair of Regenerative Medicine, Department of Tissue Engineering, Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Bydgoszcz, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Rasmus
authors.affiliation: Chair of Regenerative Medicine, Department of Tissue Engineering, Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Bydgoszcz, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Buchholz
authors.affiliation: Chair of Regenerative Medicine, Department of Tissue Engineering, Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Bydgoszcz, Poland.
authors.forename: Sandra
authors.initials: S
authors.lastname: Krzyzanowska
authors.affiliation: Department of Mechanics and Physics of Fluids, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Pawel
authors.initials: P
authors.lastname: Nakielski
authors.affiliation: Department of Mechanics and Physics of Fluids, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Chmielewski
authors.affiliation: Department of Clinical Pathomorphology, Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Bydgoszcz, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Bodnar
authors.affiliation: Department of Clinical Pathomorphology, Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Bydgoszcz, Poland; Department of Tumor Pathology, Center of Oncology, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Marszalek
authors.affiliation: Department of Pediatrics, Hematology and Oncology, Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Bydgoszcz, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Debski
authors.affiliation: Electron Microscopy Platform, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Frontczak-Baniewicz
authors.affiliation: Department of Intelligent Technologies, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Mikułowski
authors.affiliation: Chair of Regenerative Medicine, Department of Tissue Engineering, Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Bydgoszcz, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Nowacki
authors.affiliation: Department of Mechanics and Physics of Fluids, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Tomasz A
authors.initials: TA
authors.lastname: Kowalewski
authors.affiliation: Chair of Regenerative Medicine, Department of Tissue Engineering, Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Bydgoszcz, Poland; Department of Urology, Nicolaus Copernicus Hospital, Torun, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Drewa
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D007413:Intestinal Mucosa / Q000166:cytology* / Q000502:physiology; D008297:Male; D008567:Membranes, Artificial; D011091:Polyesters / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D001743:Urinary Bladder / Q000502:physiology* / Q000601:surgery
chemical_list: D008567:Membranes, Artificial; D011091:Polyesters; C062968:lactide-caprolactone copolymer