title: Use of humanized rat basophil leukemia (RBL) reporter systems for detection of allergen-specific IgE sensitization in human serum.
abstract: Determination of allergen-specific IgE levels in human blood samples is an important diagnostic technology for assessment of allergic sensitization. The presence of specific IgE in human serum samples can be measured by sensitizing humanized rat basophil leukemia (RBL) cell lines with diluted serum and measuring cellular activation after challenge with the suspected allergens. This has been traditionally performed by measuring the levels of β-hexosaminidase released upon RBL degranulation. Here, we describe the use of two recently developed humanized RBL reporter cell lines which offer higher sensitivity and are amenable to high-throughput-scale experiments.
authors.affiliation: School of Pharmacy, The University of Nottingham, Nottingham, UK.
authors.forename: Daniel
authors.initials: D
authors.lastname: Wan
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Wang
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Nakamura
authors.forename: Marcos J C
authors.initials: MJ
authors.lastname: Alcocer
authors.forename: Franco H
authors.initials: FH
authors.lastname: Falcone
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D015549:Basophil Degranulation Test / Q000379:methods*; D018929:Cell Culture Techniques / Q000379:methods; D045744:Cell Line, Tumor; D002648:Child; D006801:Humans; D006967:Hypersensitivity / Q000097:blood* / Q000175:diagnosis* / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology*; D015471:Leukemia, Basophilic, Acute / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051381:Rats
chemical_list: D000485:Allergens; D007073:Immunoglobulin E