title: Endothelial cell-derived plasmin promotes human glioma cell proliferation.
abstract: The pathogenesis of glioma is unclear. The therapeutic results are very poor currently. This study aims to investigate the endothelial cell-derived plasminogen in the promotion of glioma cell proliferation. The results showed that after exposure to polyIC, the production of plasminogen by Huvecs was markedly increased. Plasmin cleaved PAR2 on glioma cells to induce the release of IL-6 from glioma cells. Tregs differentiated into Th17(+) cells induced by IL-6 in conjunction with TGF-β in Tregs. The Th17 cells released IL-17 to facilitate the glioma cell growth. We conclude that the vascular endothelial cell-derived plasmin induces the release IL-6 from glioma cells. The IL-6 induces the production of IL-17 by Tregs. The IL-17(+) Tregs promote the glioma cell proliferation.
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Luo
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, China. Electronic address: qianxuerchen@163.com.
authors.forename: Qianxue
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Daofeng
authors.initials: D
authors.lastname: Tian
mesh_terms: D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D001932:Brain Neoplasms / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005341:Fibrinolysin / Q000378:metabolism / Q000494:pharmacology*; D005434:Flow Cytometry; D005910:Glioma / Q000473:pathology*; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D011070:Poly I-C / Q000494:pharmacology; D044464:Receptor, PAR-2 / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000187:drug effects*; D014568:Urokinase-Type Plasminogen Activator / Q000494:pharmacology; D055815:Young Adult
chemical_list: D007369:Interferon Inducers; D044464:Receptor, PAR-2; D005341:Fibrinolysin; D014568:Urokinase-Type Plasminogen Activator; D011070:Poly I-C
keywords: Apoptosis; Endothelia; Glioma; Plasminogen; Protease-activated receptor 2; TGF-β