title: Hox complex analysis through BAC recombineering.
abstract: BAC transgenesis in mice has proved to be useful in exploring the regulatory mechanisms and functions of the Hox complexes. The large constructs used may include most of the relevant components of the cis-regulatory landscape. Manipulations can be accomplished without compromising the integrity of the endogenous complex which reduces the likelihood of producing confounding phenotypic abnormalities. The development of recombineering tools has been critical in providing the means necessary to make many types of precise and varied manipulations of these large constructs. Here, we will discuss the methodologies necessary to manipulate Hox complex BACs, generation of transgenic animals bearing these constructs and the utilization of these resources to address fundamental aspects of Hox biology.
authors.affiliation: Stowers Institute for Medical Research, 1000 E 50th Street, Kansas City, MO, 64110, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Parrish
authors.forename: Youngwook
authors.initials: Y
authors.lastname: Ahn
authors.forename: Christof
authors.initials: C
authors.lastname: Nolte
authors.forename: Bony
authors.initials: B
authors.lastname: De Kumar
authors.forename: Robb
authors.initials: R
authors.lastname: Krumlauf
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D022202:Chromosomes, Artificial, Bacterial; D015870:Gene Expression; D018390:Gene Targeting; D005822:Genetic Vectors / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism*; D059765:Homologous Recombination; D019426:Integrases / Q000378:metabolism; D051379:Mice; D011995:Recombination, Genetic; D019076:Transgenes
chemical_list: D018398:Homeodomain Proteins; C045073:Cre recombinase; D019426:Integrases