title: Enhanced production of maltase (α-glucosidase) from newly isolated strain of Bacillus licheniformis KIBGE-IB4.
abstract: Maltase (alpha-glucosidase) hydrolyzes α-(1→4) glucosidic bond of maltose into two glucose molecules. It is widely used in various foods, beverages and also in textile and biofuel industries. During current study, various physicochemical parameters for maltase production from newly isolated strain of Bacillus licheniformis KIBGE-IB4 were optimized using one-factor-at-a-time methodology. It was found that Bacillus licheniformis KIBGE-IB4 produced maximum maltase at 37°C, pH-7.0 after 48 hours using wheat starch (2.5%) as carbon source along with peptone (1.0%), yeast extract (0.2%) and meat extract (0.4%) as nitrogen sources in fermentation medium. It is concluded that the optimization of various medium ingredients and conditions increases maltase production upto 6.74 fold from B. licheniformis KIBGE-IB4 as compared to previously reported media and this strain could be used for the commercial production of maltase.
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: Muhmmad Asif
authors.initials: MA
authors.lastname: Nawaz
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: Zainab
authors.initials: Z
authors.lastname: Bibi
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: Afsheen
authors.initials: A
authors.lastname: Aman
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: Raheela Rahmat
authors.initials: RR
authors.lastname: Zohra
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: Shah Ali Ul
authors.initials: SA
authors.lastname: Qader
mesh_terms: D001407:Bacillus / Q000145:classification / Q000201:enzymology*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D003470:Culture Media / Q000378:metabolism; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D013213:Starch / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D000520:alpha-Glucosidases / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D013213:Starch; D000520:alpha-Glucosidases