title: Micelle-catalyzed domain swapping in the GlpG rhomboid protease cytoplasmic domain.
abstract: Three-dimensional domain swapping is a mode of self-interaction that can give rise to altered functional states and has been identified as the trigger event in some protein deposition diseases, yet rates of interconversion between oligomeric states are usually slow, with the requirement for transient disruption of an extensive network of interactions giving rise to a large kinetic barrier. Here we demonstrate that the cytoplasmic domain of the Escherichia coli GlpG rhomboid protease undergoes slow dimerization via domain swapping and that micromolar concentrations of micelles can be used to enhance monomer-dimer exchange rates by more than 1000-fold. Detergents bearing a phosphocholine headgroup are shown to be true catalysts, with hexadecylphosphocholine reducing the 26 kcal/mol free energy barrier by >11 kcal/mol while preserving the 5 kcal/mol difference between monomer and dimer states. Catalysis involves the formation of a micelle-bound intermediate with a partially unfolded structure that is primed for domain swapping. Taken together, these results are the first to demonstrate true catalysis for domain swapping, by using micelles that work in a chaperonin-like fashion to unfold a kinetically trapped state and allow access to the domain-swapped form.
authors.affiliation: Department of Chemistry and ‡Department of Biochemistry, Microbiology and Immunology, University of Ottawa , Ottawa, Ontario, Canada K1N 6N5.
authors.forename: Houman
authors.initials: H
authors.lastname: Ghasriani
authors.forename: Jason K C
authors.initials: JK
authors.lastname: Kwok
authors.forename: Allison R
authors.initials: AR
authors.lastname: Sherratt
authors.forename: Alexander C Y
authors.initials: AC
authors.lastname: Foo
authors.forename: Tabussom
authors.initials: T
authors.lastname: Qureshi
authors.forename: Natalie K
authors.initials: NK
authors.lastname: Goto
mesh_terms: D002384:Catalysis; D002942:Circular Dichroism; D003593:Cytoplasm / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D003902:Detergents / Q000737:chemistry; D010450:Endopeptidases / Q000737:chemistry* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D008823:Micelles; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D010767:Phosphorylcholine / Q000737:chemistry; D011487:Protein Conformation; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary
chemical_list: D004268:DNA-Binding Proteins; D003902:Detergents; D029968:Escherichia coli Proteins; C102898:GlpG protein, E coli; D008565:Membrane Proteins; D008823:Micelles; D010767:Phosphorylcholine; D010450:Endopeptidases