title: Dynamic study of how the bacterial breakdown of plant cell walls allows the reconstitution of efficient hemicellulasic cocktails.
abstract: Designing more efficient mixtures of enzymes is necessary to produce molecules of interest from biomass lignocellulosic fractionation. The present study aims to investigate the strategies used by the thermophilic and hemicellulolytic bacterium Thermobacillus xylanilyticus to fractionate wheat bran and wheat straw during its growth. Results demonstrated ratios and levels of hemicellulases produced varied during growth on both biomasses. Xylanase activity was mainly produced during stationary stages of growth whereas esterase and arabinosidase activities were detected earlier. This enzymatic profile is correlated with the expression pattern of genes encoding four hemicellulases (two xylanases, one arabinosidase and one esterase) produced by T. xylanilyticus during growth. Based on identification of the bacterial strategy, the synergistic efficiency of the four hemicellulases during the hydrolysis of both substrates was evaluated. The four hemicellulases worked together with high degree of synergy and released high amounts of xylose, arabinose and phenolic acids from wheat bran and wheat straw.
authors.affiliation: Université de Reims Champagne-Ardenne, UMR614 Fractionnement des AgroRessources et Environnement, F-51100 Reims, France; INRA, UMR614 Fractionnement des AgroRessources et Environnement, F-51100 Reims, France. Electronic address: harivony.rakotoarivonina@univ-reims.fr.
authors.forename: H
authors.initials: H
authors.lastname: Rakotoarivonina
authors.affiliation: Université de Reims Champagne-Ardenne, UMR614 Fractionnement des AgroRessources et Environnement, F-51100 Reims, France; INRA, UMR614 Fractionnement des AgroRessources et Environnement, F-51100 Reims, France.
authors.forename: B
authors.initials: B
authors.lastname: Hermant
authors.affiliation: Université de Reims Champagne-Ardenne, UMR614 Fractionnement des AgroRessources et Environnement, F-51100 Reims, France; INRA, UMR614 Fractionnement des AgroRessources et Environnement, F-51100 Reims, France.
authors.forename: N
authors.initials: N
authors.lastname: Aubry
authors.affiliation: Université de Reims Champagne-Ardenne, Unité de Recherche Vignes et Vins de Champagne, EA 4707, F-51687 Reims, France.
authors.forename: F
authors.initials: F
authors.lastname: Rabenoelina
authors.affiliation: Université de Reims Champagne-Ardenne, Unité de Recherche Vignes et Vins de Champagne, EA 4707, F-51687 Reims, France.
authors.forename: F
authors.initials: F
authors.lastname: Baillieul
authors.affiliation: Université de Reims Champagne-Ardenne, UMR614 Fractionnement des AgroRessources et Environnement, F-51100 Reims, France; INRA, UMR614 Fractionnement des AgroRessources et Environnement, F-51100 Reims, France.
authors.forename: C
authors.initials: C
authors.lastname: Rémond
mesh_terms: D001089:Arabinose / Q000378:metabolism; D056449:Bacillales / Q000201:enzymology*; D002473:Cell Wall / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000096:biosynthesis; D004043:Dietary Fiber; D006026:Glycoside Hydrolases / Q000378:metabolism*; D062385:Hydroxybenzoates / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D018547:Plant Stems / Q000737:chemistry; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014908:Triticum / Q000737:chemistry*; D014994:Xylose / Q000378:metabolism
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D004043:Dietary Fiber; D062385:Hydroxybenzoates; C036909:lignocellulose; D008031:Lignin; D014994:Xylose; D001089:Arabinose; D006026:Glycoside Hydrolases; C023305:hemicellulase; C017616:phenolic acid
keywords: Biomass conversion; Biorefinery; Enzyme synergy; Hemicellulases gene expression; Thermobacillus xylanilyticus