title: New metal based drugs: spectral, electrochemical, DNA-binding, surface morphology and anticancer activity properties.
abstract: The NSAID piroxicam (PRX) drug was used for complex formation reactions with Cu(II), Zn(II) and Pt(II) metal salts have been synthesized. Then, these complexes have been characterized by spectroscopic and analytical techniques. Thermal behavior of the complexes were also investigated. The electrochemical properties of all complexes have been investigated by cyclic voltammetry (CV) using glassy carbon electrode. The biological activity of the complexes has been evaluated by examining their ability to bind to fish sperm double strand DNA (FSFSdsDNA) with UV spectroscopy. UV studies of the interaction of the PRX and its complexes with FSdsDNA have shown that these compounds can bind to FSdsDNA. The binding constants of the compounds with FSdsDNA have also been calculated. The morphology of the FSdsDNA, PRX, metal ions and metal complexes has been investigated by scanning electron microscopy (SEM). To get the SEM images, the interaction of compounds with FSdsDNA has been studied by means of differential pulse voltammetry (DPV) at FSdsDNA modified pencil graphite electrode (PGE). The decrease in intensity of the guanine oxidation signals has been used as an indicator for the interaction mechanism. The effect of proliferation PRX and complexes were examined on the HeLA and C6 cells using real-time cell analyzer with four different concentrations.
authors.affiliation: Department of Chemistry, Kahramanmaras Sutcu Imam University, 46100 Kahramanmaras, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Çeşme
authors.affiliation: Department of Chemistry, Kahramanmaras Sutcu Imam University, 46100 Kahramanmaras, Turkey. Electronic address: aysegulgolcu@gmail.com.
authors.forename: Aysegul
authors.initials: A
authors.lastname: Gölcü
authors.affiliation: Department of Chemistry, Cankırı Karatekin University, 18100 Cankırı, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Demirtaş
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004247:DNA / Q000378:metabolism*; D004560:Electricity; D004563:Electrochemistry; D004566:Electrodes; D004583:Electrons; D005399:Fishes; D006108:Graphite / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D013058:Mass Spectrometry; D008670:Metals / Q000494:pharmacology*; D010894:Piroxicam / Q000737:chemistry / Q000494:pharmacology*; D066244:Proton Magnetic Resonance Spectroscopy; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013696:Temperature
chemical_list: D000970:Antineoplastic Agents; D008670:Metals; D010894:Piroxicam; D006108:Graphite; D004247:DNA
keywords: DNA binding; Electroanalysis; Metal based drugs; Piroxicam; SEM; xCELLigence