title: A cleavable azide resin for direct click chemistry mediated enrichment of alkyne-labeled proteins.
abstract: The direct conjugation of a labeled proteome to a cleavable azide resin utilizing the copper-catalyzed azide alkyne cycloaddition is demonstrated. The procedure omits the classical streptavidin- and biotin-based affinity enrichment step and represents an operationally simpler, cheaper and less contaminated alternative for protein purification.
authors.affiliation: Department of Chemistry, Aarhus University, Langelandsgade 140, 8000 Aarhus C, Denmark. kaj@chem.au.dk.
authors.forename: Christian
authors.initials: C
authors.lastname: Sibbersen
authors.forename: Lennart
authors.initials: L
authors.lastname: Lykke
authors.forename: Niels
authors.initials: N
authors.lastname: Gregersen
authors.forename: Karl Anker
authors.initials: KA
authors.lastname: Jørgensen
authors.forename: Mogens
authors.initials: M
authors.lastname: Johannsen
mesh_terms: D000478:Alkylation; D000480:Alkynes / Q000737:chemistry*; D001386:Azides / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D002384:Catalysis; D057930:Click Chemistry; D003300:Copper / Q000737:chemistry; D061565:Cycloaddition Reaction; D012709:Serum Albumin / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism
chemical_list: D000480:Alkynes; D001386:Azides; D012709:Serum Albumin; D001710:Biotin; D003300:Copper; D019809:Streptavidin