title: Electrochemical immunosensor for anti-tissue transglutaminase antibodies based on the in situ detection of quantum dots.
abstract: A miniaturized electrochemical biosensor array with in situ detection of quantum dots (QDs) was developed for the detection of anti-transglutaminase IgG antibodies (a celiac disease biomarker) in human sera. For the fabrication of the sensor, a 8-channel screen-printed carbon electrochemical arrays were used as transducers and modified with tissue-transglutaminase by adsorption. The immunologic reaction was carried out in a few simple steps: reaction with human serum, which contains the analyte of interest, followed by the immunoreaction with anti-human IgG labeled with CdSe/ZnS QDs and electrochemical detection of Cd(2+) released from QDs. All steps were performed on the screen-printed arrays as the solid support, and the detection of Cd(2+) was performed in situ after acid attack of the QDs without a transfer step by voltammetric stripping. The electrochemical response was correlated with the anti-transglutaminase IgG concentration. The developed electrochemical immunosensor is a trustful screening tool for celiac disease diagnosis discriminating between positive and negative sera samples with high sensitivity.
authors.affiliation: Nanobioanalysis group, Department of Physical and Analytical Chemistry, University of Oviedo, 8 Julián Clavería St., Oviedo 33006, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Martín-Yerga
authors.affiliation: Nanobioanalysis group, Department of Physical and Analytical Chemistry, University of Oviedo, 8 Julián Clavería St., Oviedo 33006, Spain.
authors.forename: María Begoña
authors.initials: MB
authors.lastname: González-García
authors.affiliation: Nanobioanalysis group, Department of Physical and Analytical Chemistry, University of Oviedo, 8 Julián Clavería St., Oviedo 33006, Spain. Electronic address: costa@uniovi.es.
authors.forename: Agustín
authors.initials: A
authors.lastname: Costa-García
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000097:blood*; D015374:Biosensing Techniques; D002138:Calibration; D016022:Case-Control Studies; D002446:Celiac Disease / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D019204:GTP-Binding Proteins / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D045663:Quantum Dots / Q000032:analysis*; D011503:Transglutaminases / Q000276:immunology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; C048844:anti-IgG; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins
keywords: Celiac disease; Electrochemical biosensor; Immunosensor; Quantum dots; Screen-printed electrodes