title: Density and types of calretinin-containing retinal ganglion cells in rabbit.
abstract: Single-cell injection with lipophilic dyes following immunocytochemistry is extremely valuable for revealing the morphology of a cell expressing a protein of interest, and is a more reliable technique for cell type classification than standard morphological techniques. This study focuses on calretinin (CR), which is used as a selective marker for distinct subpopulations of neurons in the rabbit retina. The present study used single-cell injection after immunocytochemistry to describe the density and types of CR-containing retinal ganglion cells (RGCs) in rabbit. The density of CR-immunoreactive cells in the rabbit RGC cell layer was 426cells/mm(2). CR-containing RGCs were identified by immunocytochemistry and were then iontophoretically injected with a lipophilic dye, DiI. Subsequently, confocal microscope was used to characterize the morphology of CR-immunoreactive RGCs based on their dendritic field size, branching pattern, and stratification of the inner plexiform layer. Our results show that 10 morphologically different types of rabbit RGCs expressed CR. CR-containing RGCs were heterogeneous in their morphology. This approach to integrate the selective expression of a particular protein with spatial patterns of dendritic arborization will lead to a better understanding of RGCs.
authors.affiliation: Department of Ophthalmic Optic, Busan College of Information Technology, Busan 616-737, South Korea.
authors.forename: O-J
authors.initials: OJ
authors.lastname: Kwon
authors.affiliation: Department of Biology, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences and Brain Science and Engineering Institute, Kyungpook National University, Daegu 702-701, South Korea.
authors.forename: E-S
authors.initials: ES
authors.lastname: Lee
authors.affiliation: Department of Biology, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences and Brain Science and Engineering Institute, Kyungpook National University, Daegu 702-701, South Korea. Electronic address: cjjeon@knu.ac.kr.
authors.forename: C-J
authors.initials: CJ
authors.lastname: Jeon
mesh_terms: D000818:Animals; D064032:Calbindin 2 / Q000032:analysis*; D002232:Carbocyanines / Q000032:analysis; D002452:Cell Count; D005260:Female; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D011817:Rabbits; D012165:Retinal Ganglion Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: C582073:1,1'-dioleyl-3,3,3',3'-tetramethylindocarbocyanine; D064032:Calbindin 2; D002232:Carbocyanines
keywords: calretinin; density; retinal ganglion cells; single-cell injection; types