title: Anti-nociceptive and anti-inflammatory activities of the extracts of Stauntonia chinensis.
abstract: The aim of this investigation was to study the anti-nociceptive and anti-inflammatory activities of Stauntonia chinensis (S. chinensis) and the possible action mechanisms of effective fractions. The anti-nociceptive and anti-inflammatory activities of S. chinensis extracts, including the 60% EtOH extract (YMG), the n-BuOH extract (YMGB) and the aqueous residue (YMGW) of YMG, and the fractions from YMGB (YMGB1~YMGB7) were investigated by using the mouse acetic acid-induced writhing test and the rat formalin test. The effect of these extracts on the PGE2 production was tested as well. In the mouse acetic acid-induced writhing test and the rat formalin test, YMGW and YMGB displayed anti-nociceptive and anti-inflammatory activities, suggesting that they were the active ingredients of YMG. Among the fractions isolated from YMGB, YMGB1, YMGB3, YMGB4 and YMGB6 were the main active ingredients producing anti-nociceptive activity and YMGB3, YMGB5, YMGB6 and YMGB7 were the main active ingredients producing anti-inflammatory activity. Additionally, YMGW, YMGB and its separations reduced the production of PGE2, which might be the mechanism of them producing anti-inflammatory activity. These results demonstrated the active ingredients of S. chinensis producing anti-nociceptive and anti-inflammatory activities, which is valuable to validate the substance basis of S. chinensis's pharmacological actions.
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, 27th Taiping Road, Beijing, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Ying
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, 27th Taiping Road, Beijing, PR China.
authors.forename: Wu
authors.initials: W
authors.lastname: Ning
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, 27th Taiping Road, Beijing, PR China.
authors.forename: Liu
authors.initials: L
authors.lastname: Ying
authors.affiliation: Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou, PR China.
authors.forename: Gao
authors.initials: G
authors.lastname: Hao
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, 27th Taiping Road, Beijing, PR China.
authors.forename: Dong
authors.initials: D
authors.lastname: Hua-Jin
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, 27th Taiping Road, Beijing, PR China.
authors.forename: Su
authors.initials: S
authors.lastname: Rui-Bin
authors.affiliation: Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou, PR China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Xin-Sheng
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, 27th Taiping Road, Beijing, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Jin
mesh_terms: D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015232:Dinoprostone / Q000096:biosynthesis; D019073:HT29 Cells; D006801:Humans; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D029626:Ranunculaceae; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D015232:Dinoprostone