title: Authentication of pineapple (Ananas comosus [L.] Merr.) fruit maturity stages by quantitative analysis of γ- and δ-lactones using headspace solid-phase microextraction and chirospecific gas chromatography-selected ion monitoring mass spectrometry (HS-SPME-GC-SIM-MS).
abstract: Headspace solid phase microextraction and chirospecific gas chromatography-mass spectrometry in selected ion monitoring mode (HS-SPME-GC-SIM-MS) allowed quantitative determination of δ-lactones (δ-C8, δ-C10) and γ-lactones (γ-C6, γ-C8, γ-C10). A stable isotope dilution assay (SIDA) with d7-γ-decalactone as internal standard was used for quantitative analysis of pineapple lactones that was performed at three progressing post-harvest stages of fully ripe air-freighted and green-ripe sea-freighted fruits, covering the relevant shelf-life of the fruits. Fresh pineapples harvested at full maturity were characterised by γ-C6 of high enantiomeric purity remaining stable during the whole post-harvest period. In contrast, the enantiomeric purity of γ-C6 significantly decreased during post-harvest storage of sea-freighted pineapples. The biogenetical background and the potential of chirospecific analysis of lactones for authentication and quality evaluation of fresh pineapple fruits are discussed.
authors.affiliation: Institute of Food Science and Biotechnology, Chair Plant Foodstuff Technology, Hohenheim University, Garbenstrasse 25, 70599 Stuttgart, Germany. Electronic address: christof.steingass@uni-hohenheim.de.
authors.forename: Christof B
authors.initials: CB
authors.lastname: Steingass
authors.affiliation: Dienstleistungszentrum Ländlicher Raum (DLR) Rheinpfalz, Competence Centre for Wine Research, Breitenweg 71, 67435 Neustadt an der Weinstrasse, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Langen
authors.affiliation: Institute of Food Science and Biotechnology, Chair Plant Foodstuff Technology, Hohenheim University, Garbenstrasse 25, 70599 Stuttgart, Germany; King Abdulaziz University, Biological Science Department, Faculty of Science, P.O. Box 80257, Jeddah 21589, Saudi Arabia.
authors.forename: Reinhold
authors.initials: R
authors.lastname: Carle
authors.affiliation: Dienstleistungszentrum Ländlicher Raum (DLR) Rheinpfalz, Competence Centre for Wine Research, Breitenweg 71, 67435 Neustadt an der Weinstrasse, Germany.
authors.forename: Hans-Georg
authors.initials: HG
authors.lastname: Schmarr
mesh_terms: D031230:Ananas / Q000737:chemistry* / Q000254:growth & development; D005504:Food Analysis / Q000379:methods*; D005638:Fruit / Q000737:chemistry* / Q000254:growth & development; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007783:Lactones / Q000032:analysis* / Q000737:chemistry; D009812:Odorants; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D052617:Solid Phase Microextraction / Q000379:methods*; D013237:Stereoisomerism
chemical_list: D007783:Lactones; D009943:Organophosphorus Compounds; D010937:Plant Growth Regulators; C005073:ethephon
keywords: Authentication; Fruit logistics; Lactone enantiomers; Post-harvest handling