title: Carrageenan analysis. Part 2: Quantification in swine-adapted infant formula.
abstract: An LC-MS/MS method was validated in accordance with 21CFR Part 58 Good Laboratory Practice for Non-clinical Laboratory Studies to measure the concentration of carrageenan in dose formulations used in a 28-day piglet dietary feeding study of swine-adapted infant formulations stabilised with carrageenan. Carrageenan concentrations in the test formulations were 0, 300, 1000 and 2250 mg kg⁻¹ formula. The method for the measurement of carrageenan was LC-MS/MS coupled with ESI in negative-ion mode for detection. Linearity was established over the range 1.00-7.50 µg ml⁻¹. Carrageenan dose formulation samples ranging from 0 to 2250 mg kg⁻¹ of carrageenan were diluted to within the linearity range for measurement.
authors.affiliation: a Celtic Colloids Inc ., Topsham , ME , USA.
authors.forename: William R
authors.initials: WR
authors.lastname: Blakemore
authors.forename: S Karina
authors.initials: SK
authors.lastname: Kwok
authors.forename: Niles I
authors.initials: NI
authors.lastname: Harding
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000831:Animals, Newborn; D002138:Calibration; D002351:Carrageenan / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis / Q000379:methods*; D005520:Food Preservatives / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D041943:Infant Formula / Q000737:chemistry; D007231:Infant, Newborn; D009994:Osmolar Concentration; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D034421:Sus scrofa; D053719:Tandem Mass Spectrometry
chemical_list: D005520:Food Preservatives; D002351:Carrageenan
keywords: ESI; LC-MS/MS; carrageenan; infant formula; swine-adapted infant formula