title: Passive transfer of modest titers of potent and broadly neutralizing anti-HIV monoclonal antibodies block SHIV infection in macaques.
abstract: It is widely appreciated that effective human vaccines directed against viral pathogens elicit neutralizing antibodies (NAbs). The passive transfer of anti-HIV-1 NAbs conferring sterilizing immunity to macaques has been used to determine the plasma neutralization titers, which must be present at the time of exposure, to prevent acquisition of SIV/HIV chimeric virus (SHIV) infections. We administered five recently isolated potent and broadly acting anti-HIV neutralizing monoclonal antibodies (mAbs) to rhesus macaques and challenged them intrarectally 24 h later with either of two different R5-tropic SHIVs. By combining the results obtained from 60 challenged animals, we determined that the protective neutralization titer in plasma preventing virus infection in 50% of the exposed monkeys was relatively modest (∼1:100) and potentially achievable by vaccination.
authors.affiliation: Laboratory of Molecular Microbiology, Virology Laboratory, Vaccine Research Center, and Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
authors.forename: Masashi
authors.initials: M
authors.lastname: Shingai
authors.affiliation: Laboratory of Molecular Microbiology, Virology Laboratory, Vaccine Research Center, and Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
authors.forename: Olivia K
authors.initials: OK
authors.lastname: Donau
authors.affiliation: Laboratory of Molecular Microbiology, Virology Laboratory, Vaccine Research Center, and Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Plishka
authors.affiliation: Laboratory of Molecular Microbiology, Virology Laboratory, Vaccine Research Center, and Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
authors.forename: Alicia
authors.initials: A
authors.lastname: Buckler-White
authors.affiliation: Laboratory of Molecular Microbiology, Virology Laboratory, Vaccine Research Center, and Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
authors.forename: John R
authors.initials: JR
authors.lastname: Mascola
authors.affiliation: Laboratory of Molecular Microbiology, Virology Laboratory, Vaccine Research Center, and Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Nabel
authors.affiliation: Laboratory of Molecular Microbiology, Virology Laboratory, Vaccine Research Center, and Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
authors.forename: Martha C
authors.initials: MC
authors.lastname: Nason
authors.affiliation: Department of Surgery, Duke University Medical Center, Durham, NC 27710.
authors.forename: David
authors.initials: D
authors.lastname: Montefiori
authors.affiliation: Department of Immunology and Microbial Science, IAVI Neutralizing Antibody Center, and Center for HIV/AIDS Vaccine immunology and Immunogen Discovery, The Scripps Research Institute, La Jolla, CA 92037 Department of Immunology and Microbial Science, IAVI Neutralizing Antibody Center, and Center for HIV/AIDS Vaccine immunology and Immunogen Discovery, The Scripps Research Institute, La Jolla, CA 92037 Department of Immunology and Microbial Science, IAVI Neutralizing Antibody Center, and Center for HIV/AIDS Vaccine immunology and Immunogen Discovery, The Scripps Research Institute, La Jolla, CA 92037.
authors.forename: Brian
authors.initials: B
authors.lastname: Moldt
authors.affiliation: Department of Immunology and Microbial Science, IAVI Neutralizing Antibody Center, and Center for HIV/AIDS Vaccine immunology and Immunogen Discovery, The Scripps Research Institute, La Jolla, CA 92037 Department of Immunology and Microbial Science, IAVI Neutralizing Antibody Center, and Center for HIV/AIDS Vaccine immunology and Immunogen Discovery, The Scripps Research Institute, La Jolla, CA 92037 Department of Immunology and Microbial Science, IAVI Neutralizing Antibody Center, and Center for HIV/AIDS Vaccine immunology and Immunogen Discovery, The Scripps Research Institute, La Jolla, CA 92037.
authors.forename: Pascal
authors.initials: P
authors.lastname: Poignard
authors.affiliation: Department of Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel.
authors.forename: Ron
authors.initials: R
authors.lastname: Diskin
authors.affiliation: Division of Biology, California Institute of Technology, Pasadena, CA 91125 Howard Hughes Medical Institute, Chevy Chase, MD 20815.
authors.forename: Pamela J
authors.initials: PJ
authors.lastname: Bjorkman
authors.affiliation: Diagnostic and Research Services Branch, Office of the Director, National Institutes of Health, Bethesda, MD 20892.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Eckhaus
authors.affiliation: Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065.
authors.forename: Florian
authors.initials: F
authors.lastname: Klein
authors.affiliation: Laboratory of Humoral Response to Pathogens, Department of Immunology, Institut Pasteur, 75724 Paris, France.
authors.forename: Hugo
authors.initials: H
authors.lastname: Mouquet
authors.affiliation: Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065.
authors.forename: Julio Cesar
authors.initials: JC
authors.lastname: Cetrulo Lorenzi
authors.affiliation: Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065.
authors.forename: Anna
authors.initials: A
authors.lastname: Gazumyan
authors.affiliation: Department of Immunology and Microbial Science, IAVI Neutralizing Antibody Center, and Center for HIV/AIDS Vaccine immunology and Immunogen Discovery, The Scripps Research Institute, La Jolla, CA 92037 Department of Immunology and Microbial Science, IAVI Neutralizing Antibody Center, and Center for HIV/AIDS Vaccine immunology and Immunogen Discovery, The Scripps Research Institute, La Jolla, CA 92037 Department of Immunology and Microbial Science, IAVI Neutralizing Antibody Center, and Center for HIV/AIDS Vaccine immunology and Immunogen Discovery, The Scripps Research Institute, La Jolla, CA 92037 Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology and Harvard, Boston, MA 021142.
authors.forename: Dennis R
authors.initials: DR
authors.lastname: Burton
authors.affiliation: Howard Hughes Medical Institute, Chevy Chase, MD 20815 Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065.
authors.forename: Michel C
authors.initials: MC
authors.lastname: Nussenzweig
authors.affiliation: Laboratory of Molecular Microbiology, Virology Laboratory, Vaccine Research Center, and Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 malm@nih.gov ynishimura@niaid.nih.gov.
authors.forename: Malcolm A
authors.initials: MA
authors.lastname: Martin
authors.affiliation: Laboratory of Molecular Microbiology, Virology Laboratory, Vaccine Research Center, and Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 malm@nih.gov ynishimura@niaid.nih.gov.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Nishimura
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology*; D057134:Antibodies, Neutralizing / Q000008:administration & dosage / Q000276:immunology*; D017931:DNA Primers / Q000235:genetics; D006678:HIV / Q000276:immunology*; D015483:HIV Antibodies / Q000008:administration & dosage / Q000276:immunology*; D006801:Humans; D016180:Lentivirus Infections / Q000517:prevention & control*; D008253:Macaca mulatta; D016296:Mutagenesis; D009500:Neutralization Tests; D012044:Regression Analysis; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D014611:Vaccination / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D017931:DNA Primers; D015483:HIV Antibodies