title: Abnormal chemokine receptor profile on circulating T lymphocytes from nonallergic asthma patients.
abstract: BACKGROUND
  T lymphocytes are involved in the pathogenesis of nonallergic asthma. The objective of this study was to characterize the subset distribution and pattern of chemokine receptor expression in circulating T lymphocyte subsets from nonallergic asthma patients.
  
  
  METHODS
  Forty stable nonallergic asthma patients and 16 sex- and age-matched healthy donors were studied. Twelve patients did not receive inhaled steroids (untreated patients), 16 received 50-500 μg b.i.d. of inhaled fluticasone propionate (FP) (standard-dose patients), and 12 received over 500 μg b.i.d. of inhaled FP (high-dose patients) for at least 12 months prior to the beginning of this study and were clinically well controlled. Flow cytometry was performed using a panel of monoclonal antibodies (4 colors).
  
  
  RESULTS
  Nonallergic asthma patients treated with high doses of inhaled FP showed a significant reduction in the percentages of CD3+ T lymphocytes compared to healthy controls. Untreated patients showed a significant increase in CCR6 expression in CD8+CD25+ and CD8+CD25+bright T cells compared to healthy controls. The results were similar for CXCR3 and CCR5 expression. In patients treated with standard doses of FP, CCR5 expression was significantly increased in CD3+ T lymphocytes relative to healthy controls.
  
  
  CONCLUSIONS
  The different groups of clinically stable nonallergic asthmatic patients showed distinct patterns of alterations in subset distribution as well as CCR6, CXCR3, and CCR5 expression on circulating T lymphocytes. .
authors.affiliation: Department of Medicine, Universidad de Alcalá, Alcalá de Henares, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Barbarroja-Escudero
authors.forename: Alfredo
authors.initials: A
authors.lastname: Prieto-Martin
authors.forename: Jorge
authors.initials: J
authors.lastname: Monserrat-Sanz
authors.forename: Eduardo
authors.initials: E
authors.lastname: Reyes-Martin
authors.forename: David
authors.initials: D
authors.lastname: Diaz-Martin
authors.forename: Dario
authors.initials: D
authors.lastname: Antolin-Amerigo
authors.forename: Mercedes
authors.initials: M
authors.lastname: Rodriguez-Rodriguez
authors.forename: Felipe
authors.initials: F
authors.lastname: Canseco-Gonzalez
authors.forename: Leonor
authors.initials: L
authors.lastname: Kremer
authors.forename: Carlos
authors.initials: C
authors.lastname: Martinez-A
authors.forename: Melchor
authors.initials: M
authors.lastname: Alvarez-Mon
mesh_terms: D000730:Androstadienes / Q000627:therapeutic use; D001249:Asthma / Q000188:drug therapy / Q000276:immunology*; D017252:CD3 Complex / Q000096:biosynthesis; D016827:CD8 Antigens / Q000096:biosynthesis; D003430:Cross-Sectional Studies; D005260:Female; D000068298:Fluticasone; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000096:biosynthesis; D017493:Leukocyte Common Antigens; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D019713:Receptors, CCR5 / Q000096:biosynthesis*; D054399:Receptors, CCR6 / Q000096:biosynthesis*; D054367:Receptors, CXCR3 / Q000096:biosynthesis*; D012882:Skin Tests; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
chemical_list: D000730:Androstadienes; C584933:CCR5 protein, human; C106311:CCR6 protein, human; D017252:CD3 Complex; D016827:CD8 Antigens; C515187:CXCR3 protein, human; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D019713:Receptors, CCR5; D054399:Receptors, CCR6; D054367:Receptors, CXCR3; D000068298:Fluticasone; D017493:Leukocyte Common Antigens