title: Uninflatable and Notch control the targeting of Sara endosomes during asymmetric division.
abstract: Cell fate decision during asymmetric division is mediated by the biased partition of cell fate determinants during mitosis [1-6]. In the case of the asymmetric division of the fly sensory organ precursor cells, directed Notch signaling from pIIb to the pIIa daughter endows pIIa with its distinct fate [1-6]. We have previously shown that Notch/Delta molecules internalized in the mother cell traffic through Sara endosomes and are directed to the pIIa daughter [6]. Here we show that the receptor Notch itself is required during the asymmetric targeting of the Sara endosomes to pIIa. Notch binds Uninflatable, and both traffic together through Sara endosomes, which is essential to direct asymmetric endosomes motility and Notch-dependent cell fate assignation. Our data uncover a part of the core machinery required for the asymmetric motility of a vesicular structure that is essential for the directed dispatch of Notch signaling molecules during asymmetric mitosis.
authors.affiliation: Department of Biochemistry, Faculty of Sciences, University of Geneva, Quai Ernest Ansermet 30, 1211 Geneva, Switzerland.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Loubéry
authors.affiliation: Department of Biochemistry, Faculty of Sciences, University of Geneva, Quai Ernest Ansermet 30, 1211 Geneva, Switzerland.
authors.forename: Carole
authors.initials: C
authors.lastname: Seum
authors.affiliation: Department of Biochemistry, Faculty of Sciences, University of Geneva, Quai Ernest Ansermet 30, 1211 Geneva, Switzerland.
authors.forename: Ana
authors.initials: A
authors.lastname: Moraleda
authors.affiliation: Department of Biochemistry, Faculty of Sciences, University of Geneva, Quai Ernest Ansermet 30, 1211 Geneva, Switzerland.
authors.forename: Alicia
authors.initials: A
authors.lastname: Daeden
authors.affiliation: Institut de Biologie Valrose, CNRS UMR 7277, INSERM 1091, Université de Nice Sophia-Antipolis, Parc Valrose, 06108 Nice Cedex, France.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Fürthauer
authors.affiliation: Department of Biochemistry, Faculty of Sciences, University of Geneva, Quai Ernest Ansermet 30, 1211 Geneva, Switzerland. Electronic address: marcos.gonzalez@unige.ch.
authors.forename: Marcos
authors.initials: M
authors.lastname: Gonzalez-Gaitan
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000254:growth & development / Q000502:physiology*; D011992:Endosomes / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000254:growth & development / Q000502:physiology; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D016133:Polymerase Chain Reaction; D051880:Receptors, Notch / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D008565:Membrane Proteins; C497759:N protein, Drosophila; D051880:Receptors, Notch; D016212:Transforming Growth Factor beta; C545359:Uif protein, Drosophila; C515087:sara protein, Drosophila