title: From a co-production design to an integrated single-cell biorefinery.
abstract: Engineering microorganisms capable of accumulating multiple products are sometimes attractive because they yield several advantages in balancing the in vivo metabolic flux and restoring the optimal cell physiology. With the development of metabolic engineering and synthetic biology, numerous strategies for minimizing the substrate waste, optimizing the product portfolios, and maximizing the product yield in co-production systems have been designed and applied. This paper reviewed the recent developments in this field and discussed the challenges that may be encountered during the scaling up of the co-production systems. Finally, the importance of product portfolios and biorefinery strategy of single-cell in co-production processes was proposed.
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan 250100, China.
authors.forename: Quanfeng
authors.initials: Q
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan 250100, China. Electronic address: qiqingsheng@sdu.edu.cn.
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Qi
mesh_terms: D001419:Bacteria / Q000166:cytology / Q000378:metabolism; D056804:Biofuels; D001709:Biotechnology; D007218:Industrial Microbiology; D060847:Metabolic Engineering; D049490:Systems Biology
chemical_list: D056804:Biofuels
keywords: Co-production; Metabolic engineering; Microorganism; Single-cell biorefinery