title: Caseinophosphopeptides released after tryptic hydrolysis versus simulated gastrointestinal digestion of a casein-derived by-product.
abstract: The production of caseinophosphopeptides from a casein-derived by-product generated during the manufacture of a functional ingredient based on antihypertensive peptides was attempted. The casein by-product was submitted to tryptic hydrolysis for 30, 60 and 120min and further precipitated with calcium chloride and ethanol at pH 4.0, 6.0 and 8.0. Identification and semi quantification of the derived products by tandem mass spectrometry revealed some qualitative and quantitative changes in the released caseinophosphopeptides over time at the different precipitation pHs. The by-product was also subjected to simulated gastrointestinal digestion. Comparison of the resulting peptides showed large sequence homology in the phosphopeptides released by tryptic hydrolysis and simulated gastrointestinal digestion. Some regions, specifically αS1-CN 43-59, αS1-CN 60-74, β-CN 1-25 and β-CN 30-50 showed resistance to both tryptic hydrolysis and simulated digestion. The results of the present study suggest that this casein-derived by-product can be used as a source of CPPs.
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM, CEI-UAM+CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Cruz-Huerta
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM, CEI-UAM+CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: García-Nebot
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM, CEI-UAM+CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Miralles
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM, CEI-UAM+CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Recio
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM, CEI-UAM+CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain. Electronic address: lourdes.amigo@csic.es.
authors.forename: L
authors.initials: L
authors.lastname: Amigo
mesh_terms: D000595:Amino Acid Sequence; D002122:Calcium Chloride / Q000737:chemistry; D002364:Caseins / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D004063:Digestion; D000431:Ethanol / Q000737:chemistry; D041981:Gastrointestinal Tract / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010748:Phosphopeptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D013997:Time Factors; D014357:Trypsin / Q000378:metabolism
chemical_list: D002364:Caseins; D010748:Phosphopeptides; D000431:Ethanol; D014357:Trypsin; D002122:Calcium Chloride
keywords: Casein by-product; Caseinophosphopeptides; Simulated gastrointestinal digestion; Tandem mass spectrometry; Tryptic hydrolysis