title: Leech therapy for linear incisional skin-wound healing in rats.
abstract: BACKGROUND
  The aim of this study was to investigate the effects of leech therapy (LT), in comparison with topical phenytoin (PHT), on incisional skin-wound healing in animal models.
  
  
  METHODS
  This experimental study included 15 male rats (5 animals in 3 groups) with approximately equal body weights (350 ± 10 g). Skin wounds with lengths of 20 mm and depths of 0.5 mm were made on the dorsolateral region of rats 4 cm from the spine. The first group (PHT group) was treated daily with topical PHT (1%) while the second group (LT group) received LT at the beginning of the experiment. The control group received neither the drug nor the therapy. Wound healing was evaluated every day, and the study was continued until the wound had completely healed. Changes in the areas and the appearances of the skin wounds and histological differences (at the end of the experiment) were used to investigate the differences in wound healing among the groups.
  
  
  RESULTS
  The process of wound healing was significantly faster in the group treated with LT (p < 0.05) than in the group treated with the PHT.
  
  
  CONCLUSIONS
  The study results showed that LT improved incisional skin-wound healing in rats.
authors.affiliation: Department of Zoology, School of Biology, Faculty of Science, University of Tehran, Tehran, Iran.
authors.forename: Kaveh Darabi
authors.initials: KD
authors.lastname: Darestani
authors.affiliation: Department of Physiology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran. Electronic address: mirghazanfari@tums.ac.ir.
authors.forename: Sayid Mahdi
authors.initials: SM
authors.lastname: Mirghazanfari
authors.affiliation: Department of Pathology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran.
authors.forename: Katayoun Gohari
authors.initials: KG
authors.lastname: Moghaddam
authors.affiliation: Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Hejazi
mesh_terms: D000818:Animals; D007865:Leeches; D024763:Leeching; D008297:Male; D010672:Phenytoin / Q000494:pharmacology; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects / Q000293:injuries; D014945:Wound Healing
chemical_list: D010672:Phenytoin
keywords: leech therapy; phenytoin; rat; skin wound