title: Genome sequence and organization analysis of Heliothis virescens ascovirus 3f isolated from a Helicoverpa zea larva.
abstract: The complete genome sequence of Heliothis virescens ascovirus 3f (HvAV-3f) was obtained. The HvAV-3f genome has a circular genome of 198,157bp with a G+C content of 46.0%, and encodes 190 open reading frames (ORFs) longer than 69 amino acids. Two major homologous regions (hrs) and 29 'baculovirus repeat ORFs' (bro) were found in the genome. BLAST analyses revealed that three HvAV-3f genes were homologous to that of lepidopteran insects. Nine ORFs were unique to HvAV-3f, in which two ORFs showed significant levels of similarity to genes that have not been previously described for ascoviruses in the Genbank database.
authors.affiliation: Institute of Virology, Hunan Agricultural University, Changsha, Hunan 410128, China; Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan 410128, China.
authors.forename: Yong-Lu
authors.initials: YL
authors.lastname: Wei
authors.affiliation: Institute of Virology, Hunan Agricultural University, Changsha, Hunan 410128, China; Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan 410128, China.
authors.forename: Jue
authors.initials: J
authors.lastname: Hu
authors.affiliation: Institute of Virology, Hunan Agricultural University, Changsha, Hunan 410128, China; Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan 410128, China.
authors.forename: Shun-Ji
authors.initials: SJ
authors.lastname: Li
authors.affiliation: Institute of Virology, Hunan Agricultural University, Changsha, Hunan 410128, China; Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan 410128, China.
authors.forename: Zi-Shu
authors.initials: ZS
authors.lastname: Chen
authors.affiliation: Institute of Virology, Hunan Agricultural University, Changsha, Hunan 410128, China; Department of Microbiology, Miami University, 32 Pearson Hall, Oxford, OH 45056, USA. Electronic address: Chengx@MiamiOH.edu.
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Cheng
authors.affiliation: Institute of Virology, Hunan Agricultural University, Changsha, Hunan 410128, China; Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan 410128, China. Electronic address: tineidaehgh@gmail.com.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Huang
mesh_terms: D000818:Animals; D029223:Ascoviridae / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D016679:Genome, Viral / Q000235:genetics*; D007814:Larva / Q000821:virology; D017422:Sequence Analysis, DNA; D014481:United States; D003313:Zea mays / Q000821:virology*
chemical_list: D004279:DNA, Viral
keywords: Bro genes; Gene transfer; Genome organization; Repeat sequences