title: E-selectin gene (S128R) polymorphism in hemorrhagic stroke: comparison with ischemic stroke.
abstract: Increasing evidence suggests that genetic variation in inflammatory genes plays a pivotal role in pathogenesis of stroke. The aim of the present study was to evaluate the association of E-selectin S128R polymorphism with hemorrhagic stroke and also to evaluate the genotypic and allelic variation with ischemic stroke in a South Indian population from Andhra Pradesh. In this study, we recruited 250 hemorrhagic stroke patients along with 250 age and sex matched controls. The genotypes were determined using PCR-RFLP method and the strength of association between genotypes and hemorrhagic stroke was determined by odds ratio with 95% confidence interval (CI) and chi-square analysis. Allelic and genotypic frequencies of the polymorphism differed significantly between hemorrhagic stroke patients and controls (p<0.001). Significant association was also found following dominant (p<0.001) and co-dominant (p<0.001) models. On comparing the genotypic and allelic frequencies between ischemic and hemorrhagic stroke significant difference was found between the two stroke types (p<0.001). In conclusion, we found the AC genotype to be a significant risk factor for hemorrhagic stroke and we also found significant differences in AC genotype and C allele among the two stroke types. The genotypic and allelic variation between the ischemic and hemorrhagic stroke, suggests that E-selectin S128R mediated amplification of leukocytes onto endothelial cells, leading to secondary damage of brain cells is more pronounced in hemorrhagic stroke.
authors.affiliation: Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad 500016, India; Dr. NTR University of Health Sciences, Vijayawada, Andhra Pradesh, India.
authors.forename: Satrupa
authors.initials: S
authors.lastname: Das
authors.affiliation: Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad 500016, India; Dr. NTR University of Health Sciences, Vijayawada, Andhra Pradesh, India.
authors.forename: Sitara
authors.initials: S
authors.lastname: Roy
authors.affiliation: Nizam's Institute of Medical Sciences, Punjagutta, Hyderabad 500082, India.
authors.forename: Subhash
authors.initials: S
authors.lastname: Kaul
authors.affiliation: Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad 500016, India.
authors.forename: Akka
authors.initials: A
authors.lastname: Jyothy
authors.affiliation: Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad 500016, India; Centre for Human Genetics, School of Health Sciences, Central University of Punjab, Bathinda, Punjab 151001, India. Electronic address: anjanadurani@yahoo.co.in.
authors.forename: Anjana
authors.initials: A
authors.lastname: Munshi
mesh_terms: D002545:Brain Ischemia / Q000235:genetics*; D019040:E-Selectin / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007194:India; D020300:Intracranial Hemorrhages / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012307:Risk Factors; D020521:Stroke / Q000235:genetics*
chemical_list: D019040:E-Selectin; C546936:SELE protein, human
keywords: Cytokine; E-selectin; Genetics; Hemorrhagic stroke; Ischemic stroke; Polymorphism