title: Enhanced osteogenic potential of human mesenchymal stem cells on electrospun nanofibrous scaffolds prepared from eri-tasar silk fibroin.
abstract: This study evaluated the mechanical properties and osteogenic potential of a silk fibroin scaffold prepared from a 70:30 blend of Eri (Philosamia ricini) and Tasar (Antheraea mylitta) silk, respectively (ET scaffolds). An electrospinning process was used to prepare uniformly blended, fibrous scaffolds of nanoscale dimensions, as confirmed by scanning and transmission electron microscopy (fiber diameter < 300 nm). Similarly prepared scaffolds derived from gelatin and Bombyx mori (BM) silk fibroin were used as controls. Mechanical testing and atomic force microscopy showed that the ET scaffolds had significantly higher tensile strength (1.83 ± 0.13 MPa) and surface roughness (0.44 μm) compared with BM (1.47 ± 0.10 MPa; 0.37 μm) and gelatin scaffolds (0.6 ± 0.07 MPa; 0.28 μm). All scaffolds were exposed to mesenchymal stem cells isolated to human chord blood (hMSCs) for up to 28 days in vitro. Alamar blue and alkaline phosphatase assay showed greater attachment and proliferation for both ET and BM scaffolds compared with gelatin. The ET scaffolds also promoted greater differentiation of the attached hMSCs as evidenced by higher expression of RunX2, osteocalcin, and CD29/CD44 expression. ET scaffolds also showed significantly higher mineralization, as evidenced by glycosaminoglycan assay, alizarin red staining, and elemental analysis of crystalline composites isolated from the scaffolds.
authors.affiliation: Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela-769008, Odisha, India.
authors.forename: Niladri Nath
authors.initials: NN
authors.lastname: Panda
authors.affiliation: Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela-769008, Odisha, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Biswas
authors.affiliation: Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela-769008, Odisha, India.
authors.forename: Krishna
authors.initials: K
authors.lastname: Pramanik
authors.affiliation: Department of Pharmaceutical Sciences, Philadelphia College of Pharmacy, USciences, Philadelphia, Pennsylvania, 19104.
authors.forename: Sriramakamal
authors.initials: S
authors.lastname: Jonnalagadda
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis*; D002448:Cell Adhesion; D002454:Cell Differentiation; D005349:Fibroins / Q000737:chemistry*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D009036:Moths; D010012:Osteogenesis; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000943:Antigens, Differentiation; D005349:Fibroins
keywords: bioactive material; nanomaterials/nanophase; osteogenesis; scaffolds; stem cells