title: Assessment of the E-selectin rs5361 (561A>C) polymorphism and soluble protein concentration in acute coronary syndrome: association with circulating levels.
abstract: BACKGROUND
  The acute coronary syndrome (ACS) is a complex disease where genetic and environmental factors are involved. E-selectin gene is a candidate for ACS progression due to its contribution in the inflammatory process and endothelial function. The rs5361 (561A>C) polymorphism in the E-selectin gene has been linked to changes in gene expression, affinity for its receptor, and plasmatic levels; therefore it is associated with an increased risk of cardiovascular disease. The aim of this study was to determine the association of the rs5361 polymorphism with ACS and to measure serum levels of soluble E-selectin (sE-selectin).
  
  
  METHODS
  283 ACS patients and 205 healthy subjects (HS) from Western Mexico were included. The polymerase chain reaction-restriction fragment length polymorphism was used to determine the rs5361 polymorphism. The sE-selectin levels were measured by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Neither genotype nor allele frequencies of the rs5361 polymorphism showed statistical differences between groups. The sE-selectin levels were significantly higher in ACS patients compared to HS (54.58 versus 40.41 ng/ml, P = 0.02). The C allele had no effect on sE-selectin levels.
  
  
  CONCLUSIONS
  The rs5361 E-selectin gene polymorphism is not a susceptibility marker for ACS in Western Mexico population. However, sE-selectin may be a biological marker of ACS.
authors.affiliation: Centro Universitario de Ciencias de la Salud, UdeG. Sierra Mojada 950, 44340 Guadalajara, JAL, Mexico ; Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Edificio Q, Primer Piso, Colonia Independencia, 44340 Guadalajara, JAL, Mexico.
authors.forename: Elena
authors.initials: E
authors.lastname: Sandoval-Pinto
authors.affiliation: Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Edificio Q, Primer Piso, Colonia Independencia, 44340 Guadalajara, JAL, Mexico.
authors.forename: Jorge Ramon
authors.initials: JR
authors.lastname: Padilla-Gutiérrez
authors.affiliation: Centro Universitario de Ciencias de la Salud, UdeG. Sierra Mojada 950, 44340 Guadalajara, JAL, Mexico ; Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Edificio Q, Primer Piso, Colonia Independencia, 44340 Guadalajara, JAL, Mexico.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Valdes-Alvarado
authors.affiliation: Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Edificio Q, Primer Piso, Colonia Independencia, 44340 Guadalajara, JAL, Mexico ; Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Edificio Q, Primer Piso, Colonia Independencia, 44340 Guadalajara, JAL, Mexico.
authors.forename: Ilian Janet
authors.initials: IJ
authors.lastname: García-González
authors.affiliation: Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Edificio Q, Primer Piso, Colonia Independencia, 44340 Guadalajara, JAL, Mexico ; Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Edificio Q, Primer Piso, Colonia Independencia, 44340 Guadalajara, JAL, Mexico.
authors.forename: Angelica
authors.initials: A
authors.lastname: Valdez-Haro
authors.affiliation: Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Edificio Q, Primer Piso, Colonia Independencia, 44340 Guadalajara, JAL, Mexico.
authors.forename: Jose Francisco
authors.initials: JF
authors.lastname: Muñoz-Valle
authors.affiliation: IMSS, Centro Médico Nacional de Occidente, Belisario Dominguez 1000, Colonia Independencia, 44340 Guadalajara, JAL, Mexico.
authors.forename: Hector Enrique
authors.initials: HE
authors.lastname: Flores-Salinas
authors.affiliation: Hospital General de Occidente, Secretaria de Salud Jalisco, Avenida Zoquipan 1050, Colonia Zoquipan, 45170 Zapopan, JAL, Mexico.
authors.forename: Fernando
authors.initials: F
authors.lastname: Rivas
authors.affiliation: Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Edificio Q, Primer Piso, Colonia Independencia, 44340 Guadalajara, JAL, Mexico.
authors.forename: Yeminia
authors.initials: Y
authors.lastname: Valle
mesh_terms: D054058:Acute Coronary Syndrome / Q000097:blood* / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019040:E-Selectin / Q000097:blood* / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*
chemical_list: D019040:E-Selectin