title: Molecular mechanism driving retroperitoneal adipocyte hypertrophy and hyperplasia in response to a high-sugar diet.
abstract: METHODS
  We have previously shown an increase in adipocyte size and lipid content in retroperitoneal white adipose tissue (rWAT) induced by an 8-week high-sugar diet (HSD). In this study, we assessed the effect of a HSD on the transcriptional activity of adipogenic genes in a time-course study to provide insight regarding the genetic networks involved in the rWAT response to dietary sugar.
  
  
  RESULTS
  Weaned male Wistar rats were fed a standard chow diet or HSD (68% carbohydrates) for 4, 8 or 12 weeks, and rWAT was removed for histopathology and PCR array (adipogenesis) analyses. The HSD induced adipocyte hypertrophy and hyperplasia in rWAT after 12 weeks of ingestion. Additionally, the HSD altered serum VLDL-cholesterol, triacylglycerol and glucometabolic parameters. Hierarchical clustering revealed HSD-induced changes in the expression patterns of the tested gene set. Pathway analysis, which used the enrichment analysis algorithm of the Thompson Reuters MetaCore platform, associated a cluster of differentially expressed genes with canonical pathways related to regulating adipocyte differentiation and proliferation (p-value < 10(-7)).
  
  
  CONCLUSIONS
  HSD feeding post-weaning increased both the adipocyte size and number by simultaneously up-regulating pro-adipogenic signals (the PPARγ pathway) and down-regulating anti-adipogenic signals (Wnt pathway) in young adults.
authors.affiliation: Laboratório de Bioquímica e Biologia Molecular, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Brasil.
authors.forename: Karina Barbosa
authors.initials: KB
authors.lastname: de Queiroz
authors.forename: Roney Santos
authors.initials: RS
authors.lastname: Coimbra
authors.forename: Amanda Rios
authors.initials: AR
authors.lastname: Ferreira
authors.forename: Cláudia Martins
authors.initials: CM
authors.lastname: Carneiro
authors.forename: Nívia Carolina Nogueira
authors.initials: NC
authors.lastname: Paiva
authors.forename: Daniela Caldeira
authors.initials: DC
authors.lastname: Costa
authors.forename: Elísio Alberto
authors.initials: EA
authors.lastname: Evangelista
authors.forename: Renata
authors.initials: R
authors.lastname: Guerra-Sá
mesh_terms: D017667:Adipocytes / Q000187:drug effects*; D050156:Adipogenesis / Q000187:drug effects; D000818:Animals; D001823:Body Composition / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049108:Cell Enlargement; D049109:Cell Proliferation / Q000187:drug effects; D015243:Cholesterol, VLDL / Q000097:blood; D019422:Dietary Sucrose / Q000008:administration & dosage* / Q000009:adverse effects; D015536:Down-Regulation; D006965:Hyperplasia / Q000378:metabolism; D006984:Hypertrophy / Q000378:metabolism; D050152:Intra-Abdominal Fat / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D008297:Male; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood; D015854:Up-Regulation; D060449:Wnt Signaling Pathway
chemical_list: D015243:Cholesterol, VLDL; D019422:Dietary Sucrose; D047495:PPAR gamma; D014280:Triglycerides
keywords: High-sugar diet; Insulin resistance; PCR array; Systems biology; Unsupervised machine learning