title: The "one-step" Bean pod mottle virus (BPMV)-derived vector is a functional genomics tool for efficient overexpression of heterologous protein, virus-induced gene silencing and genetic mapping of BPMV R-gene in common bean (Phaseolus vulgaris L.).
abstract: BACKGROUND
  Over the last two years, considerable advances have been made in common bean (Phaseolus vulgaris L.) genomics, especially with the completion of the genome sequence and the availability of RNAseq data. However, as common bean is recalcitrant to stable genetic transformation, much work remains to be done for the development of functional genomics tools adapted to large-scale studies.
  
  
  RESULTS
  Here we report the successful implementation of an efficient viral vector system for foreign gene expression, virus-induced gene silencing (VIGS) and genetic mapping of a BPMV resistance gene in common bean, using a "one-step" BPMV vector originally developed in soybean. With the goal of developing this vector for high-throughput VIGS studies in common bean, we optimized the conditions for rub-inoculation of infectious BPMV-derived plasmids in common bean cv. Black Valentine. We then tested the susceptibility to BPMV of six cultivars, and found that only Black Valentine and JaloEEP558 were susceptible to BPMV. We used a BPMV-GFP construct to detect the spatial and temporal infection patterns of BPMV in vegetative and reproductive tissues. VIGS of the PHYTOENE DESATURASE (PvPDS) marker gene was successfully achieved with recombinant BPMV vectors carrying fragments ranging from 132 to 391 bp. Finally, we mapped a gene for resistance to BPMV (R-BPMV) at one end of linkage group 2, in the vicinity of a locus (I locus) previously shown to be involved in virus resistance.
  
  
  CONCLUSIONS
  The "one-step" BPMV vector system therefore enables rapid and simple functional studies in common bean, and could be suitable for large-scale analyses. In the post-genomic era, these advances are timely for the common bean research community.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Pflieger
authors.forename: Sophie
authors.initials: S
authors.lastname: Blanchet
authors.forename: Chouaib
authors.initials: C
authors.lastname: Meziadi
authors.forename: Manon M S
authors.initials: MM
authors.lastname: Richard
authors.forename: Vincent
authors.initials: V
authors.lastname: Thareau
authors.forename: Fanny
authors.initials: F
authors.lastname: Mary
authors.forename: Céline
authors.initials: C
authors.lastname: Mazoyer
authors.affiliation: CNRS, Institut de Biologie des Plantes, UMR 8618, Université Paris Sud, Saclay Plant Sciences (SPS), Orsay, 91405, France. valerie.geffroy@u-psud.fr.
authors.forename: Valérie
authors.initials: V
authors.lastname: Geffroy
mesh_terms: D002874:Chromosome Mapping; D060467:Disease Resistance / Q000235:genetics; D020868:Gene Silencing; D018390:Gene Targeting; D005822:Genetic Vectors; D023281:Genomics; D027805:Phaseolus / Q000235:genetics* / Q000821:virology; D010641:Phenotype; D010942:Plant Viruses