title: Properties and fibroblast cellular response of soft and hard thermoplastic polyurethane electrospun nanofibrous scaffolds.
abstract: Soft and hard thermoplastic polyurethane (TPU) and their blends were electrospun to fabricate nanofibrous scaffolds with various properties in order to investigate the substrate property effects on cellular response. The scaffolds were characterized with Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, water contact angle tests, and protein absorption tests. It was found that the hard segment content in the scaffold increased with the hard TPU ratio, which resulted in improved hydrophobicity and decreased over all protein absorption. 3T3 fibroblasts were cultured on those scaffolds to investigate the cellular response. On soft TPU scaffolds, the cells formed were round in shape and aggregated into clusters. However, on hard TPU scaffolds, the cells exhibited a spindle shape and spread out on the scaffolds, indicating preferred cell-substrate interaction. The cell viability and proliferation of cells on hard scaffolds were higher than on soft scaffolds and on 50% hard/50% soft scaffolds.
authors.affiliation: The Key Laboratory for Polymer Processing Engineering of Ministry of Education, South China University of Technology, Guangzhou, 510640, China.|Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, Wisconsin, 53715.|Department of Mechanical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, 53706.
authors.forename: Hao-Yang
authors.initials: HY
authors.lastname: Mi
authors.affiliation: The Key Laboratory for Polymer Processing Engineering of Ministry of Education, South China University of Technology, Guangzhou, 510640, China.|Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, Wisconsin, 53715.|Department of Mechanical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, 53706.
authors.forename: Xin
authors.initials: X
authors.lastname: Jing
authors.affiliation: Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, Wisconsin, 53715.|Department of Engineering Physics, University of Wisconsin-Madison, Wisconsin, 53706.
authors.forename: Max R
authors.initials: MR
authors.lastname: Salick
authors.affiliation: Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, Wisconsin, 53715.
authors.forename: Travis M
authors.initials: TM
authors.lastname: Cordie
authors.affiliation: The Key Laboratory for Polymer Processing Engineering of Ministry of Education, South China University of Technology, Guangzhou, 510640, China.
authors.forename: Xiang-Fang
authors.initials: XF
authors.lastname: Peng
authors.affiliation: Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, Wisconsin, 53715.|Department of Mechanical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, 53706.
authors.forename: Lih-Sheng
authors.identifier: http://orcid.org/0000-0001-8022-9224
authors.initials: LS
authors.lastname: Turng
mesh_terms: D016475:3T3 Cells; D000818:Animals; D048430:Cell Shape; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011140:Polyurethanes
keywords: electrospinning; fibroblast; polyurethane; protein absorption; wettability