title: [Vitro study on gene transfection efficiency of hyaluronic acid modified core-shell liponanoparticles in human retinal pigment epithelium cells].
abstract: The aim of this study is to prepare hyaluronic acid (HA) modified core-shell liponanoparticles (pHA-LCS-NPs) as gene delivery system and investigate its gene transfection efficiency in human retinal pigment epithelium (ARPE-19) cells in vitro. The pHA-LCS-NPs was prepared by firstly hydrating dry lipid film with CS-NPs suspension to get LCS-NPs, then modifying the lipid bilayer with HA by amidation reaction between HA and dioleoyl phosphatidylethanolamine (DOPE). Its morphology, particle size and zeta potential were investigated. XTT assay was used to evaluate the cell safety of different vectors in vitro. The gene transfection efficiency of pHA-LCS-NPs modified with different contents of HA was investigated in ARPE-19 cells with green fluorescent protein (pEGFP) as the reporter gene. The results showed that the obtained pHA-LCS-NPs exhibited a clear core-shell structure with the average particles size of (214.9 +/- 7.2) nm and zeta potential of (-35 +/- 3.7) mV. The 24 h cumulative release of gene from pHA-LCS-NPs was less than 30%. After 48 h incubation, gene transfection efficiency of pHA-LCS-NPs/pEGFP was 1.81 times and 3.75 times higher than that of CS-NPs/pEGFP and naked pEGFP, respectively. Also no obvious cytotoxicity was observed on pHA-LCS-NPs. It suggested that the pHA-LCS-NPs might be promising non-viral gene delivery systems with high efficiency and low cytotoxicity.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Gan
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jia
authors.forename: Yong
authors.initials: Y
authors.lastname: Gan
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Liu
mesh_terms: D002470:Cell Survival; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology*; D008055:Lipids; D053758:Nanoparticles; D010316:Particle Size; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000494:pharmacology*; D055213:Retinal Pigment Epithelium / Q000187:drug effects*; D014162:Transfection
chemical_list: D008055:Lipids; D010714:Phosphatidylethanolamines; D049452:Green Fluorescent Proteins; C094877:dioleoyl phosphatidylethanolamine; D006820:Hyaluronic Acid