title: Clonal differences in IgE antibodies affect cutaneous anaphylaxis-associated thermal sensitivity in mice.
abstract: Cellular and molecular mediators of immune responses are increasingly implicated in acute and chronic pain pathophysiologies. Here we demonstrate that passive cutaneous IgE/Ag anaphylaxis provokes increased thermal sensitivity in the hind paw tissue of mice. The murine anti-DNP IgE antibodies SPE-7 and ɛ26 are known to induce differential cytokine production in bone marrow cultured mast cells in vitro without antigen challenge. We found a novel, antigen-dependent heterogeneity in the thermal pain responses elicited in the hind paws between SPE-7 and ɛ26 sensitized DNP-challenged mice. Mice experienced pronounced hind paw thermal sensitivity lasting 6h after DNP challenge when sensitized with SPE-7 but not ɛ26 IgE. The two IgE clones induced equivalent hind paw edema, neutrophil influx, cytokine production, and reduction in tissue histamine content in vivo, and bound to the same or overlapping epitopes on the DNP antigen in vitro. Therefore IgE antibodies against the same antigen can induce comparable inflammation, yet contribute to markedly different anaphylaxis-associated pain within an allergic response, suggesting that non-canonical IgE binding partners such as sensory neurons may play a role in allergy-related pain responses.
authors.affiliation: Macalester College, Department of Biology, Saint Paul, MN 55105, United States.
authors.forename: Madison
authors.initials: M
authors.lastname: Mack
authors.affiliation: Macalester College, Department of Biology, Saint Paul, MN 55105, United States.
authors.forename: Elena
authors.initials: E
authors.lastname: Tonc
authors.affiliation: Macalester College, Department of Biology, Saint Paul, MN 55105, United States.
authors.forename: Alyssa
authors.initials: A
authors.lastname: Ashbaugh
authors.affiliation: Macalester College, Department of Biology, Saint Paul, MN 55105, United States.
authors.forename: Abigail
authors.initials: A
authors.lastname: Wetzel
authors.affiliation: Macalester College, Department of Biology, Saint Paul, MN 55105, United States.
authors.forename: Akilah
authors.initials: A
authors.lastname: Sykes
authors.affiliation: Macalester College, Department of Biology, Saint Paul, MN 55105, United States.
authors.forename: Camilla
authors.initials: C
authors.lastname: Engblom
authors.affiliation: Macalester College, Department of Biology, Saint Paul, MN 55105, United States.
authors.forename: Estela
authors.initials: E
authors.lastname: Shabani
authors.affiliation: Macalester College, Department of Biology, Saint Paul, MN 55105, United States.
authors.forename: Carolina
authors.initials: C
authors.lastname: Mora-Solano
authors.affiliation: Macalester College, Department of Biology, Saint Paul, MN 55105, United States.
authors.forename: Anna
authors.initials: A
authors.lastname: Trier
authors.affiliation: Macalester College, Department of Biology, Saint Paul, MN 55105, United States.
authors.forename: Linnea
authors.initials: L
authors.lastname: Swanson
authors.affiliation: Macalester College, Department of Biology, Saint Paul, MN 55105, United States.
authors.forename: Emily
authors.initials: E
authors.lastname: Ewan
authors.affiliation: Macalester College, Department of Biology, Saint Paul, MN 55105, United States.
authors.forename: Tijana
authors.initials: T
authors.lastname: Martinov
authors.affiliation: Macalester College, Department of Biology, Saint Paul, MN 55105, United States. Electronic address: chatterjead@macalester.edu.
authors.forename: Devavani
authors.initials: D
authors.lastname: Chatterjea
mesh_terms: D000485:Allergens / Q000009:adverse effects / Q000276:immunology*; D000818:Animals; D000941:Antigens / Q000009:adverse effects / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D000939:Epitopes / Q000276:immunology / Q000378:metabolism; D006636:Histamine Release / Q000276:immunology; D006358:Hot Temperature; D006941:Hyperesthesia / Q000209:etiology*; D007073:Immunoglobulin E / Q000009:adverse effects / Q000276:immunology*; D008297:Male; D051379:Mice; D020556:Neutrophil Infiltration / Q000276:immunology; D010323:Passive Cutaneous Anaphylaxis / Q000235:genetics / Q000276:immunology*; D011485:Protein Binding / Q000276:immunology; D013997:Time Factors
chemical_list: D000485:Allergens; D000941:Antigens; D016207:Cytokines; D000939:Epitopes; D007073:Immunoglobulin E
keywords: Cytokinergic IgE; Passive cutaneous anaphylaxis; Thermal pain