title: Odontogenic differentiation of human dental pulp cells by calcium silicate materials stimulating via FGFR/ERK signaling pathway.
abstract: Bone healing needs a complex interaction of growth factors that establishes an environment for efficient bone formation. We examine how calcium silicate (CS) and tricalcium phosphate (β-TCP) cements influence the behavior of human dental pulp cells (hDPCs) through fibroblast growth factor receptor (FGFR) and active MAPK pathways, in particular ERK. The hDPCs are cultured with β-TCP and CS, after which the cells' viability and odontogenic differentiation markers are determined by using PrestoBlue® assay and western blot, respectively. The effect of small interfering RNA (siRNA) transfection targeting FGFR was also evaluated. The results showed that CS promoted cell proliferation and enhances FGFR expression. It was also found that CS increases ERK and p38 activity in hDPCs, and furthermore, raises the expression and secretion of DSP, and DMP-1. Additionally, statistically significant differences (p<0.05) have been found in the calcium deposition in si-FGFR transfection and ERK inhibitor between CS and β-TCP; these variations indicated that ERK/MAPK signaling is involved in the silicon-induced odontogenic differentiation of hDPCs. The current study shows that CS substrates play a key role in odontoblastic differentiation of hDPCs through FGFR and modulate ERK/MAPK activation.
authors.affiliation: School of Dentistry, Chung Shan Medical University, Taichung City, Taiwan.
authors.forename: Chao-Hsin
authors.initials: CH
authors.lastname: Liu
authors.affiliation: School of Dentistry, Chung Shan Medical University, Taichung City, Taiwan; Department of Dentistry, Chung Shan Medical University Hospital, Taichung City, Taiwan.
authors.forename: Chi-Jr
authors.initials: CJ
authors.lastname: Hung
authors.affiliation: School of Dentistry, Chung Shan Medical University, Taichung City, Taiwan; Department of Dentistry, Chung Shan Medical University Hospital, Taichung City, Taiwan.
authors.forename: Tsui-Hsien
authors.initials: TH
authors.lastname: Huang
authors.affiliation: Department of Chemical and Materials Engineering, Tunghai University, Taichung City, Taiwan.
authors.forename: Chi-Chang
authors.initials: CC
authors.lastname: Lin
authors.affiliation: School of Dentistry, Chung Shan Medical University, Taichung City, Taiwan; Department of Dentistry, Chung Shan Medical University Hospital, Taichung City, Taiwan.
authors.forename: Chia-Tze
authors.initials: CT
authors.lastname: Kao
authors.affiliation: Department of Chemical and Materials Engineering, Tunghai University, Taichung City, Taiwan. Electronic address: eviltacasi@gmail.com.
authors.forename: Ming-You
authors.initials: MY
authors.lastname: Shie
mesh_terms: D001483:Base Sequence; D001672:Biocompatible Materials; D017610:Calcium Compounds / Q000737:chemistry / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D018360:Crystallography, X-Ray; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics / Q000378:metabolism*; D017640:Silicates / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D017610:Calcium Compounds; D034741:RNA, Small Interfering; D017468:Receptors, Fibroblast Growth Factor; D017640:Silicates; D048049:Extracellular Signal-Regulated MAP Kinases; C031293:calcium silicate
keywords: Calcium silicate cement; Fibroblast growth factor receptor; Human dental pulp cell; MAPK; Odontogenesis