title: Polymorphism of the IL-8 gene and the risk of ovarian cancer.
abstract: Ovarian cancer still represents a challenge in gynecological oncology. Most patients are diagnosed in an advanced tumor stage. No specific screening or prevention strategies for ovarian cancer exist as of yet. Interleukin 8 (IL-8) is a pro-inflammatory chemokine known for its angiogenetic activity, and is supposedly responsible for tumor-associated angiogenesis in several malignant tumors. The aim of the study was to investigate the susceptibility of patients with an IL-8 gene polymorphism to developing ovarian cancer. Four single nucleotide polymorphisms (SNPs) (IL-8 -251, IL-8 +781, IL-8 +1633 and IL-8 +2767) of the IL-8 gene were screened, using the PCR method in 268 patients with ovarian cancer and 426 healthy women as a control group. Significant associations were noted in patients with the IL-8 +781 (T/T) genotype (p=0.0048) with increased frequencies of ovarian cancer, while women with the IL-8 +781 (C/C) allele suffer from ovarian cancer significantly less frequently (p=0.0003). Furthermore, the IL-8 +2767 (T/T) genotype is also associated with a higher risk of ovarian cancer (p=0.0177). Our results indicate, for the first time, that IL-8 polymorphism is associated with ovarian cancer.
authors.affiliation: Department of Gynecology and Obstetrics, Medical University Greifswald, Ferdinand-Sauerbruch-Str.,17475 Greifswald, Germany. Electronic address: koensgen@uni-greifswald.de.
authors.forename: D
authors.initials: D
authors.lastname: Koensgen
authors.affiliation: Department of Gynecology and Obstetrics, Medical University Greifswald, Ferdinand-Sauerbruch-Str.,17475 Greifswald, Germany. Electronic address: daniela.bruennert@uni-greifswald.de.
authors.forename: D
authors.initials: D
authors.lastname: Bruennert
authors.affiliation: Department of Gynecology and Obstetrics, Medical University Greifswald, Ferdinand-Sauerbruch-Str.,17475 Greifswald, Germany; Department of Gynecology, Oncological Institute, Testemitanu Str. 30, 2025 Chisinau, Moldavia. Electronic address: svungureanu@yahoo.com.
authors.forename: S
authors.initials: S
authors.lastname: Ungureanu
authors.affiliation: Department of Gynecology, Oncological Institute, Testemitanu Str. 30, 2025 Chisinau, Moldavia. Electronic address: sofroni@yandex.ru.
authors.forename: D
authors.initials: D
authors.lastname: Sofroni
authors.affiliation: Department of Gynecology, European Competence Center for Ovarian Cancer, Campus Virchow Klinikum, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. Electronic address: elena.braicu@charite.de.
authors.forename: E I
authors.initials: EI
authors.lastname: Braicu
authors.affiliation: Department of Gynecology, European Competence Center for Ovarian Cancer, Campus Virchow Klinikum, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. Electronic address: jalid.sehouli@charite.de.
authors.forename: J
authors.initials: J
authors.lastname: Sehouli
authors.affiliation: Institute for Immunology and Transfusion Medicine, Ferdinand-Sauerbruch-Str., 17475 Greifswald, Germany. Electronic address: asuemnig@uni-greifswald.de.
authors.forename: A
authors.initials: A
authors.lastname: Sümnig
authors.affiliation: Department of Gynecology and Obstetrics, Medical University Greifswald, Ferdinand-Sauerbruch-Str.,17475 Greifswald, Germany. Electronic address: delogus@uni-greifswald.de.
authors.forename: S
authors.initials: S
authors.lastname: Delogu
authors.affiliation: Department of Gynecology and Obstetrics, Medical University Greifswald, Ferdinand-Sauerbruch-Str.,17475 Greifswald, Germany. Electronic address: zygmunt@uni-greifswald.de.
authors.forename: M
authors.initials: M
authors.lastname: Zygmunt
authors.affiliation: Department of Gynecology and Obstetrics, Medical University Greifswald, Ferdinand-Sauerbruch-Str.,17475 Greifswald, Germany. Electronic address: pankaj_bio@curaj.ac.in.
authors.forename: P
authors.initials: P
authors.lastname: Goyal
authors.affiliation: Institute for Pathology, Medical University Greifswald, Friedrich-Loeffler-Str. 23e, 17487 Greifswald, Germany. Electronic address: matthias.evert@uni-greifswald.de.
authors.forename: M
authors.initials: M
authors.lastname: Evert
authors.affiliation: Epiontis GmbH, Rudower Chaussee 29, 12489 Berlin, Germany. Electronic address: sven.olek@eptiontis.com.
authors.forename: S
authors.initials: S
authors.lastname: Olek
authors.affiliation: Institute for Biometrics and Medical Informatics, Walther-Rathenau-Straße 48, 17475 Greifswald, Germany. Electronic address: biebler@biometrie.uni-greifswald.de.
authors.forename: K E
authors.initials: KE
authors.lastname: Biebler
authors.affiliation: Department of Gynecology and Obstetrics, Medical University Greifswald, Ferdinand-Sauerbruch-Str.,17475 Greifswald, Germany. Electronic address: mustea@uni-greifswald.de.
authors.forename: A
authors.initials: A
authors.lastname: Mustea
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001483:Base Sequence; D016022:Case-Control Studies; D016207:Cytokines / Q000378:metabolism; D005060:Europe; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D008875:Middle Aged; D008969:Molecular Sequence Data; D009389:Neovascularization, Pathologic; D010051:Ovarian Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D055815:Young Adult
chemical_list: C508606:CXCL8 protein, human; D016207:Cytokines; D016209:Interleukin-8
keywords: IL-8 polymorphism; Ovarian cancer; PCR