title: miR-202 suppresses proliferation and induces apoptosis of osteosarcoma cells by downregulating Gli2.
abstract: Accumulating data have shown that microRNAs are involved in the pathogenesis of cancer. miR-202 has been confirmed to be downregulated in several types of human cancer. However, the expression and biological role of miR-202 in osteosarcoma (OS) carcinogenesis and progression remain unclear. In this study, we demonstrated that miR-202 expression is significantly decreased in human OS cell lines and specimens. Restoration of miR-202 expression could inhibit OS cell proliferation, induce cell apoptosis, and suppress tumor growth in nude mice models. We subsequently identified the transcription factor Gli2 as a direct target of miR-202. Overexpression of Gli2 blocked the inhibitory function of miR-202. Taken together, our results indicate that miR-202 acts as a novel tumor suppressor to regulate OS cell proliferation and apoptosis through downregulating Gli2 expression.
authors.affiliation: Department of Traumatology, Yantaishan Hospital, Yantai, 264025, Shandong Province, China.
authors.forename: Zhengwen
authors.initials: Z
authors.lastname: Sun
authors.forename: Tongqing
authors.initials: T
authors.lastname: Zhang
authors.forename: Huanyu
authors.initials: H
authors.lastname: Hong
authors.forename: Qingxia
authors.initials: Q
authors.lastname: Liu
authors.forename: Haiguang
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D064593:Heterografts; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009368:Neoplasm Transplantation; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D000075746:Zinc Finger Protein Gli2
chemical_list: C082593:GLI2 protein, human; D051741:Kruppel-Like Transcription Factors; C569392:MIRN202 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D012334:RNA, Neoplasm; D000075746:Zinc Finger Protein Gli2