title: BP8, a novel peptide from avian immune system, modulates B cell developments.
abstract: The bursa of Fabricius (BF) is the key humoral immune organ unique to birds, and is critical for early B-lymphocyte proliferation and differentiation. However, the molecular basis and mechanisms through which the BF regulates B cell development are not fully understood. In this study, we isolated and identified a new bursal peptide (BP8, AGHTKKAP) by RP-HPLC and MALDI-TOF-MS. BP8 promoted colony-forming pre-B formation, bound B cell precursor, regulated B cell development in vitro as well as in vivo, upstream of the EBF-E2A-Pax5 regulatory complex and increased immunoglobulin secretion. These data revealed a bursal-derived multifunctional factor BP8 as a novel biomaterial which is essential for the development of the immune system. This study elucidates further the mechanisms involved in humoral immune system and has implications in treating human diseases.
authors.affiliation: Division of Key Lab of Animal Disease Diagnosis and Immunology of China's Department of Agriculture, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Feng
authors.forename: Rui-Bing
authors.initials: RB
authors.lastname: Cao
authors.forename: Pu-Yan
authors.initials: PY
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D002060:Bursa of Fabricius / Q000737:chemistry* / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002645:Chickens; D005260:Female; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D010455:Peptides