title: A normative study of the synovial fluid proteome from healthy porcine knee joints.
abstract: Synovial fluid in an articulating joint contains proteins derived from the blood plasma and proteins that are produced by cells within the joint tissues, such as synovium, cartilage, ligament, and meniscus. The proteome composition of healthy synovial fluid and the cellular origins of many synovial fluid components are not fully understood. Here, we present a normative proteomics study using porcine synovial fluid. Using our optimized method, we identified 267 proteins with high confidence in healthy synovial fluid. We also evaluated mRNA expression data from tissues that can contribute to the synovial fluid proteome, including synovium, cartilage, blood, and liver, to better estimate the relative contributions from these sources to specific synovial fluid components. We identified 113 proteins in healthy synovial fluid that appear to be primarily derived from plasma transudates, 37 proteins primarily derived from synovium, and 11 proteins primarily derived from cartilage. Finally, we compared the identified synovial fluid proteome to the proteome of human plasma, and we found that the two body fluids share many similarities, underlining the detected plasma derived nature of many synovial fluid components. Knowing the synovial fluid proteome of a healthy joint will help to identify mechanisms that cause joint disease and pathways involved in disease progression.
authors.affiliation: Department of Pathology and Proteomics Center, ‡Department of Orthopaedic Surgery, §Department of Urology, and ∥Howard Hughes Medical Institute, Boston Children's Hospital , Boston, Massachusetts 02115, United States.
authors.forename: Tue
authors.initials: T
authors.lastname: Bennike
authors.forename: Ugur
authors.initials: U
authors.lastname: Ayturk
authors.forename: Carla M
authors.initials: CM
authors.lastname: Haslauer
authors.forename: John W
authors.initials: JW
authors.lastname: Froehlich
authors.forename: Benedikt L
authors.initials: BL
authors.lastname: Proffen
authors.forename: Omar
authors.initials: O
authors.lastname: Barnaby
authors.forename: Svend
authors.initials: S
authors.lastname: Birkelund
authors.forename: Martha M
authors.initials: MM
authors.lastname: Murray
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Warman
authors.forename: Allan
authors.initials: A
authors.lastname: Stensballe
authors.forename: Hanno
authors.initials: H
authors.lastname: Steen
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D007719:Knee Joint / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D020543:Proteome; D012333:RNA, Messenger / Q000235:genetics; D013552:Swine; D013556:Swine, Miniature; D013582:Synovial Fluid / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D011506:Proteins; D020543:Proteome; D012333:RNA, Messenger
keywords: PTM; Synovial fluid; human; method optimization; origin; plasma; porcine; proteomics; synovium; transcriptomics