title: Effects of age-related loss of P/Q-type calcium channels in a mice model of peripheral nerve injury.
abstract: We analyzed the role of P/Q-type calcium channels in sciatic nerve regeneration after lesion induced by chronic constriction injury (CCI) in heterozygous null mutant mice lacking the CaV2.1α1 subunit of these channels (Cacna1a+/-). Compared with wild type, Cacna1a+/- mice showed an initial reduction of the CCI-induced allodynia, indicating a reduced pain perception, but they also evidenced a lack of recovery over time, with atrophy of the injured hindpaw still present 3 months after CCI when wild-type mice fully recovered. In parallel, Cacna1a+/- mice exhibited an early onset of age-dependent loss of P/Q-type channels, which can be responsible for the lack of functional recovery. Moreover, Cacna1a+/- mice showed an early age-dependent reduction of muscular strength, as well as of Schwann cells proliferation and sciatic nerve remyelination. This study demonstrates the important role played by P/Q-type channels in recovery from nerve injury and has important implications for the knowledge of age-related processes.
authors.affiliation: CNR National Research Council of Italy, Cell Biology and Neurobiology Institute, IRCCS Santa Lucia Foundation, Rome, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Marinelli
authors.affiliation: CNR National Research Council of Italy, Cell Biology and Neurobiology Institute, IRCCS Santa Lucia Foundation, Rome, Italy.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Eleuteri
authors.affiliation: CNR National Research Council of Italy, Cell Biology and Neurobiology Institute, IRCCS Santa Lucia Foundation, Rome, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Vacca
authors.affiliation: CNR National Research Council of Italy, Cell Biology and Neurobiology Institute, IRCCS Santa Lucia Foundation, Rome, Italy.
authors.forename: Georgios
authors.initials: G
authors.lastname: Strimpakos
authors.affiliation: CNR National Research Council of Italy, Cell Biology and Neurobiology Institute, IRCCS Santa Lucia Foundation, Rome, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Mattei
authors.affiliation: CNR National Research Council of Italy, Cell Biology and Neurobiology Institute, IRCCS Santa Lucia Foundation, Rome, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Severini
authors.affiliation: CNR National Research Council of Italy, Cell Biology and Neurobiology Institute, IRCCS Santa Lucia Foundation, Rome, Italy.
authors.forename: Flaminia
authors.initials: F
authors.lastname: Pavone
authors.affiliation: CNR National Research Council of Italy, Cell Biology and Neurobiology Institute, IRCCS Santa Lucia Foundation, Rome, Italy. Electronic address: siro.luvisetto@cnr.it.
authors.forename: Siro
authors.initials: S
authors.lastname: Luvisetto
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D020865:Calcium Channels, P-Type / Q000172:deficiency* / Q000378:metabolism* / Q000502:physiology; D020907:Calcium Channels, Q-Type / Q000172:deficiency* / Q000502:physiology; D004195:Disease Models, Animal; D008815:Mice, Inbred Strains; D009416:Nerve Regeneration / Q000502:physiology; D059348:Peripheral Nerve Injuries / Q000378:metabolism* / Q000503:physiopathology; D012584:Sciatic Nerve / Q000378:metabolism / Q000502:physiology
chemical_list: D020865:Calcium Channels, P-Type; D020907:Calcium Channels, Q-Type
keywords: Allodynia; Cacna1a; Muscle; Nerve regeneration; P/Q-type channels; Schwann cells