title: Templated assembly of albumin-based nanoparticles for simultaneous gene silencing and magnetic resonance imaging.
abstract: In this article, we address the design of innovative human serum albumin (HSA)-based nanoparticles loaded with silencing RNA and grafted with gadolinium complexes having average sizes ranging from ca. 50 to 150 nm according to the siRNA/HSA composition. The non-covalent siRNA/HSA assembly is formed on isobutyramide-modified mesoporous silica and the self-supported HSA-based nanoparticles are obtained following the silica template dissolution. These original protein particles provide simultaneous magnetic resonance imaging contrast enhancement and cellular in vitro gene silencing.
authors.affiliation: Institut de Physique et Chimie des Matériaux de Strasbourg (IPCMS), UMR 7504, CNRS, Université de Strasbourg, 23, rue du Loess, BP 43, 67034 Strasbourg Cedex 2, France. damien.mertz@ipcms.unistra.fr.
authors.forename: Damien
authors.initials: D
authors.lastname: Mertz
authors.forename: Christine
authors.initials: C
authors.lastname: Affolter-Zbaraszczuk
authors.forename: Julien
authors.initials: J
authors.lastname: Barthès
authors.forename: Jiwei
authors.initials: J
authors.lastname: Cui
authors.forename: Frank
authors.initials: F
authors.lastname: Caruso
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Baumert
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Voegel
authors.forename: Joelle
authors.initials: J
authors.lastname: Ogier
authors.forename: Florent
authors.initials: F
authors.lastname: Meyer
mesh_terms: D045744:Cell Line, Tumor; D004705:Endocytosis; D005682:Gadolinium / Q000737:chemistry; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry; D006801:Humans; D046911:Macromolecular Substances; D008279:Magnetic Resonance Imaging; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D016062:Porosity; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D006573:Heterocyclic Compounds, 1-Ring; D046911:Macromolecular Substances; D034741:RNA, Small Interfering; D012709:Serum Albumin; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid; D012822:Silicon Dioxide; D005682:Gadolinium