title: Enhancement of chlorogenic acid production in hairy roots of Platycodon grandiflorum by over-expression of an Arabidopsis thaliana transcription factor AtPAP1.
abstract: To improve the production of chlorogenic acid (CGA) in hairy roots of Platycodon grandiflorum, we induced over-expression of Arabidopsis thaliana transcription factor production of anthocyanin pigment (AtPAP1) using an Agrobacterium rhizogenes-mediated transformation system. Twelve hairy root lines showing over-expression of AtPAP1 were generated. In order to investigate the regulation of AtPAP1 on the activities of CGA biosynthetic genes, the expression levels of seven P. grandiflorum CGA biosynthetic genes were analyzed in the hairy root line that had the greatest accumulation of AtPAP1 transcript, OxPAP1-1. The introduction of AtPAP1 increased the mRNA levels of all examined CGA biosynthetic genes and resulted in a 900% up-regulation of CGA accumulation in OxPAP1-1 hairy roots relative to controls. This suggests that P. grandiflorum hairy roots that over-express the AtPAP1 gene are a potential alternative source of roots for the production of CGA.
authors.affiliation: Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-Gu, Daejeon 305-764, Korea. tuan_pham_6885@yahoo.com.
authors.forename: Pham Anh
authors.initials: PA
authors.lastname: Tuan
authors.affiliation: Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-Gu, Daejeon 305-764, Korea. kdy309@naver.com.
authors.forename: Do Yeon
authors.initials: DY
authors.lastname: Kwon
authors.affiliation: Department of Integrative Plant Science, Chung-Ang University, Anseong 456-756, Korea. slee@cau.ac.kr.
authors.forename: Sanghyun
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia. mvalanarasu@gmail.com.
authors.forename: Mariadhas Valan
authors.initials: MV
authors.lastname: Arasu
authors.affiliation: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia. naldhabi@ksu.edu.sa.
authors.forename: Naif Abdullah
authors.initials: NA
authors.lastname: Al-Dhabi
authors.affiliation: Department of Plant Science, Gangneung-Wonju National University, 7 Jukheon-gil, Gangneung-si, Gangwon-do 210-702, Korea. nipark@gwnu.ac.kr.
authors.forename: Nam Il
authors.initials: NI
authors.lastname: Park
authors.affiliation: Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-Gu, Daejeon 305-764, Korea. supark@cnu.ac.kr.
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Park
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002726:Chlorogenic Acid / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D031238:Platycodon / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D002726:Chlorogenic Acid; D014157:Transcription Factors; C502612:AT1G56650 protein, Arabidopsis