title: Detection of the interaction between host and bacterial proteins: eukaryotic nucleolin interacts with Francisella elongation factor Tu.
abstract: Dissecting the interaction between bacterial and host proteins is fundamental in understanding pathogenesis. It is also very helpful for exploring new therapeutic approaches, either preventive or curative. Here, we describe different techniques, which allowed us to detect new molecules involved in the binding and infection of the bacterium Francisella tularensis, on human cells. This facultative intracellular pathogen is the causative agent of tularemia and is considered as a bio-threatening agent. The privileged host cells are monocytes and macrophages. We used both "in vitro" and "in vivo" experiments to explore the modulation of F. tularensis infection and thereafter determine a bacterial ligand and its host receptor molecule.
authors.affiliation: INSERM 1151 Team 11, Unité de Pathogénie des Infections Systémiques, Université Paris Descartes, Bâtiment Leriche, 14 rue Maria Helena Vieira Da Silva CS61431, 75993, Paris cedex 14, France, monique.barel@inserm.fr.
authors.forename: Monique
authors.initials: M
authors.lastname: Barel
authors.forename: Alain
authors.initials: A
authors.lastname: Charbit
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D005604:Francisella tularensis / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D006801:Humans; D010444:Peptide Elongation Factor Tu / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D010444:Peptide Elongation Factor Tu