title: Target-site basis for resistance to acetolactate synthase inhibitor in Water chickweed (Myosoton aquaticum L.).
abstract: Water chickweed is a widespread and competitive winter annual or biennial weed of wheat in China. One Water chickweed population (HN02) resistant to several acetolactate synthase (ALS) inhibitors was found in Henan province of China. Whole-plant bioassays showed that HN02 was high resistance to tribenuron (292.05-flod). In vitro ALS assays revealed that resistance was due to reduced sensitivity of the ALS enzyme to tribenuron. The I50 value for HN02 was 85.53 times greater respectively than that of susceptible population (SD05). This altered ALS sensitivity in the resistant population was due to a mutation in the ALS gene resulting in a Pro197 to Ser substitution. Cross-resistance experiments indicated that HN02 exhibited various resistance patterns to pyrithiobac-sodium, florasulam and pyroxsulam, without resistance to imazethapyr. This is the first report of tribenuron-resistant Water chickweed in Henan province of China, target-site based resistance was established as being due to an insensitive form of ALS, resulting from a Pro to Ser substitution at amino acid position 197 in the ALS gene.
authors.affiliation: Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong, Tai'an 271018, PR China.
authors.forename: Weitang
authors.initials: W
authors.lastname: Liu
authors.affiliation: Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong, Tai'an 271018, PR China; College of Plant Science, Anhui Science and Technology University, Anhui, Fengyang 233100, PR China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong, Tai'an 271018, PR China; College of Chemistry and Pharmacy Science, Qingdao Agricultural University, Shandong, Qingdao 266109, PR China.
authors.forename: Lingxu
authors.initials: L
authors.lastname: Li
authors.affiliation: Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong, Tai'an 271018, PR China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong, Tai'an 271018, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Du
authors.affiliation: Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong, Tai'an 271018, PR China. Electronic address: wangjx@sdau.edu.cn.
authors.forename: Jinxin
authors.initials: J
authors.lastname: Wang
mesh_terms: D000095:Acetolactate Synthase / Q000037:antagonists & inhibitors* / Q000235:genetics; D019943:Amino Acid Substitution; D001190:Arylsulfonates / Q000494:pharmacology*; D018744:DNA, Plant / Q000235:genetics; D053209:Herbicide Resistance / Q000235:genetics; D006540:Herbicides / Q000494:pharmacology*; D010940:Plant Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D011392:Proline / Q000235:genetics; D012694:Serine / Q000235:genetics; D029752:Stellaria / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics
chemical_list: D001190:Arylsulfonates; D018744:DNA, Plant; D006540:Herbicides; D010940:Plant Proteins; C050296:metsulfuron methyl; D012694:Serine; D011392:Proline; D000095:Acetolactate Synthase
keywords: ALS enzyme; ALS gene; ALS inhibitor; Herbicide resistance; Water chickweed