title: A capacitive biosensor based on an interdigitated electrode with nanoislands.
abstract: A capacitive biosensor based on an interdigitated electrode (IDE) with nanoislands was developed for label-free detection of antigen-antibody interactions. To enable sensitive capacitive detection of protein adsorption, the nanoislands were fabricated between finger electrodes of the IDE. The effect of the nanoislands on the sensitive capacitive measurement was estimated using horseradish peroxidase (HRP) as a model protein. Additionally, a parylene-A film was coated on the IDE with nanoislands to improve the efficiency of protein immobilization. By using HRP and hepatitis B virus surface antigen (HBsAg) as model analytes, the effect of the parylene-A film on the capacitive detection of protein adsorption was demonstrated.
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-749, South Korea.
authors.forename: Ha-Wook
authors.initials: HW
authors.lastname: Jung
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-749, South Korea.
authors.forename: Young Wook
authors.initials: YW
authors.lastname: Chang
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-749, South Korea.
authors.forename: Ga-yeon
authors.initials: GY
authors.lastname: Lee
authors.affiliation: Department of Biomedical Engineering, Gachon University, Incheon 406-799, South Korea.
authors.forename: Sungbo
authors.initials: S
authors.lastname: Cho
authors.affiliation: Korea Institute of Science and Technology (KIST), Seoul, South Korea.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Kang
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-749, South Korea. Electronic address: jcpyun@yonsei.ac.kr.
authors.forename: Jae-Chul
authors.initials: JC
authors.lastname: Pyun
mesh_terms: D015374:Biosensing Techniques; D004566:Electrodes; D004800:Enzymes, Immobilized; D006735:Horseradish Peroxidase; D007118:Immunoassay; D049329:Nanostructures
chemical_list: D004800:Enzymes, Immobilized; D006735:Horseradish Peroxidase
keywords: Capacitive biosensor; Hepatitis B virus; Immunosensor; Interdigitated electrode; Nanoisland; Surface antigen