title: Development of an indirect ELISA with epitope on nonstructural protein of Muscovy duck parvovirus for differentiating between infected and vaccinated Muscovy ducks.
abstract: The aim of this study was to develop an indirect enzyme-linked immunosorbent assay (i-ELISA) based on epitope AA503-509 (RANEPKE), which is on nonstructural protein of Muscovy duck parvovirus (MDPV). Sera (100) from negative and vaccinated Muscovy ducks were compared with infected sera (240) to establish the cut-off value of this i-ELISA. There was a significant difference between the positive and negative populations (P < 0·05). The adoption of this positive-negative threshold value for this i-ELISA assay resulted in specificity of 98·0%. This i-ELISA could be used as a diagnostic tool for differentiating infected Muscovy ducks from Muscovy ducks vaccinated with inactivated virus.
  
  
  CONCLUSIONS
  In this study, we developed an i-ELISA based on epitope AA503-509 (RANEPKE), which is on nonstructural protein of MDPV. This i-ELISA could be used as a diagnostic tool for differentiating infected Muscovy ducks from Muscovy ducks vaccinated with inactivated virus.
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, China; College of Life Science and Agriculture Forestry, Qiqihar University, Qiqihar, China.
authors.forename: B
authors.initials: B
authors.lastname: Yan
authors.forename: J-Z
authors.initials: JZ
authors.lastname: Ma
authors.forename: T-F
authors.initials: TF
authors.lastname: Yu
authors.forename: S-L
authors.initials: SL
authors.lastname: Shao
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: X-D
authors.initials: XD
authors.lastname: Fan
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001715:Bird Diseases / Q000175:diagnosis*; D004372:Ducks / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D000939:Epitopes; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D017992:Parvovirus / Q000276:immunology*; D012680:Sensitivity and Specificity; D014611:Vaccination / Q000662:veterinary*; D017361:Viral Nonstructural Proteins / Q000276:immunology; D014765:Viral Vaccines
chemical_list: D000914:Antibodies, Viral; D000939:Epitopes; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines
keywords: ELISA; Muscovy duck parvovirus; differential diagnosis; epitope; nonstructural protein