title: Molecular cloning and expression of glutathione S-transferases involved in propargite resistance of the carmine spider mite, Tetranychus cinnabarinus (Boisduval).
abstract: The carmine spider mite (CSM) Tetranychus cinnabarinus has become a serious pest in China and has developed resistance to acaricide propargite as it is used to control mites worldwide including T. cinnabarinus. In this study, a resistant colony of T. cinnabarinus, PRR34 (37.78-fold resistant ratio), was established after 34 generations of propargite selection, and cross-resistance patterns of 7 other acaricides were determined in comparison with a susceptible strain (SS). The contribution of detoxification enzymes to propargite tolerance were investigated using biological, biochemical and molecular approaches. Enzyme inhibitor synergist tests suggested glutathione S-transferases (GST) involvement in propargite-resistance of PRR34, and GST activity against 1-chloro-2,4-dinitrobenzene (CDNB) was correlated with the development of resistance. Eight novel GST genes (TcGSTd1, TcGSTd2, TcGSTm1, TcGSTm2, TcGSTm3, TcGSTm4 and TcGSTm5) were cloned, and phylogenetic analysis showed that the eight GST genes were most closely related to GST family delta and mu from Tetranychusurticae. Quantitative RT-PCR revealed that the expression level of GSTs in PPR34 strain increased in larvae, nymphs and adults, while decreased in eggs compared with that of SS. Collectively, these results support a role of GSTs in mediating resistance to propargite in the PRR34 strain. TcGSTd1,TcGSTd2 and TcGSTm2 genes might play significant roles in propargite resistance of CSM, especially at adult stage. This is the first attempt to define specific genes involved in GST mediated propargite resistance of T. cinnabarinus at the transcriptional level.
authors.affiliation: Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi, China. Electronic address: yanjieluo@126.com.
authors.forename: Yan-Jie
authors.initials: YJ
authors.lastname: Luo
authors.affiliation: Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi, China.
authors.forename: Zhen-Guo
authors.initials: ZG
authors.lastname: Yang
authors.affiliation: Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi, China.
authors.forename: Dao-Yan
authors.initials: DY
authors.lastname: Xie
authors.affiliation: College of Plant Protection, Southwest University, Chongqing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ding
authors.affiliation: Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi, China.
authors.forename: Ai-Si
authors.initials: AS
authors.lastname: Da
authors.affiliation: Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ni
authors.affiliation: Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi, China.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Chai
authors.affiliation: Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
authors.affiliation: Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi, China.
authors.forename: Xiu-Jun
authors.initials: XJ
authors.lastname: Jiang
authors.affiliation: Institute of Crop, Yunnan Academy of Agricultural Sciences, Kunming, China.
authors.forename: Shao-Xiang
authors.initials: SX
authors.lastname: Li
mesh_terms: D056810:Acaricides / Q000633:toxicity*; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003510:Cyclohexanes / Q000633:toxicity*; D018076:DNA, Complementary / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics*; D007928:Lethal Dose 50; D008969:Molecular Sequence Data; D040102:Tetranychidae / Q000187:drug effects* / Q000235:genetics*
chemical_list: D056810:Acaricides; D060829:Arthropod Proteins; D003510:Cyclohexanes; D018076:DNA, Complementary; C002771:Omite; D005982:Glutathione Transferase
keywords: Acaricide resistance; Gene expression; Glutathione S-transferases; Propargite; Tetranychus cinnabarinus