title: Skeletal muscle stem cells for muscle regeneration.
abstract: Adult skeletal muscle possesses remarkable regenerative capacity. Muscle regeneration is mediated by a rare population of muscle stem cells that reside between the basal lamina and sarcolemma of muscle fibers. Due to their anatomical location, muscle stem cells have been coined satellite cells. Here, we describe a method that we routinely use to isolate large and pure populations of satellite cells from skeletal muscles enabling studies on autonomous properties of satellite cells to unravel the role of muscle stem cells in tissue regeneration.
authors.affiliation: Department of Cardiac Development and Remodelling, Max-Planck-Institute for Heart and Lung Research, Ludwigstraße 43, 61231, Bad Nauheim, Germany.
authors.forename: Johnny
authors.initials: J
authors.lastname: Kim
authors.forename: Thomas
authors.initials: T
authors.lastname: Braun
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D005434:Flow Cytometry; D016130:Immunophenotyping; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology*; D012038:Regeneration; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism