title: Preclinical Evaluation of Antiurolithiatic Activity of Viburnum opulus L. on Sodium Oxalate-Induced Urolithiasis Rat Model.
abstract: The aim of the present research is to evaluate the antiurolithiatic effect of the various extracts prepared from the fruits of Viburnum opulus L., in regard to its ethnobotanical record. To induce urolithiasis, 70 mg/kg sodium oxalate was injected to the rats which were housed individually in metabolic cages. The test materials were applied during 7 days. Biochemical (urine and serum parameters), histopathological and antioxidant (TBARs, TSH and GSH) assays were conducted. The urine samples were examined by light microscope for the determination of the calcium oxalate crystals. Lyophilized juice of V. opulus (LJVO) and lyophilized commercial juice of V. opulus (LCJVO) exerted potential antiurolithiatic activity which was attributed to its diuretic effect along with the inhibitory action on the oxalate levels and free radical production. We also determined the chlorogenic acid content of the LJVO by high-performance liquid chromatography (HPLC). Chlorogenic acid was determined by using Supelcosil LC-18 (250 × 4.6 mm, 5 µm) column and acetonitrile: water: 0.2% o-phosphoric acid as a mobile phase. The chlorogenic acid content of V. opulus was found to be 0.3227 mg/mL in fruit juice. The results obtained in this study have provided a scientific evidence for the traditional usage of V. opulus on passing kidney stones in Turkish folk medicine.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler, 06330 Ankara, Turkey.
authors.forename: Mert
authors.initials: M
authors.lastname: Ilhan
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100 Ankara, Turkey.
authors.forename: Burçin
authors.initials: B
authors.lastname: Ergene
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler, 06330 Ankara, Turkey.
authors.forename: Ipek
authors.initials: I
authors.lastname: Süntar
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100 Ankara, Turkey.
authors.forename: Serkan
authors.initials: S
authors.lastname: Ozbilgin
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100 Ankara, Turkey.
authors.forename: Gülçin
authors.initials: G
authors.lastname: Saltan Çitoğlu
authors.affiliation: Laboratory Animals Breeding and Experimental Researches Center, Faculty of Pharmacy, Gazi University, Etiler, 06330 Ankara, Turkey.
authors.forename: M Ayşe
authors.initials: MA
authors.lastname: Demirel
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe University, 03200 Afyonkarahisar, Turkey.
authors.forename: Hikmet
authors.initials: H
authors.lastname: Keleş
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100 Ankara, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Altun
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler, 06330 Ankara, Turkey.
authors.forename: Esra
authors.initials: E
authors.lastname: Küpeli Akkol