title: The Pro12Ala Polymorphism of PPAR-γ Gene Is Associated with Sepsis Disease Severity and Outcome in Chinese Han Population.
abstract: Peroxisome proliferator-activated receptor-γ (PPAR-γ) is a ligand-binding nuclear receptor, and its activation plays a prominent role in regulating the inflammatory response. Therefore, PPAR-γ has been suggested as a candidate gene for sepsis. In the present study, we investigated the association between the Pro12Ala polymorphism of PPAR-γ and sepsis in a Han Chinese population. A total of 308 patients with sepsis and 345 healthy controls were enrolled in this study. Genotyping was performed using the polymerase chain reaction-ligation detection reaction (PCR-LDR) method. No significant differences were detected in the allele and genotype distributions of the PPAR-γ Pro12Ala SNP between septic patients and controls (P = 0.622 for genotype; P = 0.629 for allele). However, stratification by subtypes (sepsis, septic shock, and severe sepsis) revealed a statistically significant difference in the frequency of the Ala allele and Ala-carrier genotype between the patients with the sepsis subtype and the healthy controls (P = 0.014 for allele and P = 0.012, for genotype). Moreover, significant differences were found in the frequency of the Ala allele and genotype between the sepsis survivors and nonsurvivors (all P = 0.002). In the survivors, the PPAR-γ Pro12Ala genotype was significantly associated with decreased disease severity and recovery time (all P < 0.001). Thus, genetic polymorphism is thought to play a role in the development and outcome of sepsis.
authors.affiliation: Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Guoda
authors.initials: G
authors.lastname: Ma
authors.affiliation: Department of Vascular Surgery of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Guixi
authors.initials: G
authors.lastname: Mo
authors.affiliation: Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Cui
authors.affiliation: Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: You
authors.initials: Y
authors.lastname: Li
authors.affiliation: Intensive Care Unit, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Intensive Care Unit, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Yuliu
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Intensive Care Unit, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Jiawu
authors.initials: J
authors.lastname: Fu
authors.affiliation: Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Tao
authors.affiliation: Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Liangqing
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.
authors.forename: Keshen
authors.initials: K
authors.lastname: Li