title: Molecular evolution, characterization and expression profiling of uterine aldoketoreductase 1B5 gene in endometrium of goat (Capra hircus).
abstract: Aldoketoreductase 1B5 (AKR1B5), a member of the Aldoketoreductase family, is involved in the production of Prostaglandin F2α (PGF2α) as one of vital prostaglandin F synthase (PGFS). PGs (Prostaglandins) play a crucial role in female reproductive system. In the present study, we cloned and characterized the full-length open reading frame of AKR1B5 gene in Black Bengal (BB) goat. The complete coding sequence of AKR1B5 comprises an entire open reading frame of 951 bp, encoding 316 amino acid (AA) residues. BB AKR1B5 showed >82.9% identity with that of cattle, rabbit, human, and rat at nucleotide and amino acid levels, respectively. Further, a systematic study of AKR1B5 sequence evolution was also conducted using Phylogenetic Analysis by Maximum Likelihood (PAML), entropy plot, and Blossum 62 in a phylogenetic context. Analysis of nonsynonymous to synonymous nucleotide substitution rate ratios (Ka/Ks) revealed that negative selection may have been operating on this gene during evolution in goat, cattle, rabbit, human, and rat, which showed its conservation across species. Further, expression of AKR1B5 was determined by quantitative real-time PCR in goat endometrial tissues at different stages of the estrous cycle and early pregnancy. Our results indicated its high expression at luteolytic phase (stage III; day 16-21) during the estrous cycle. However, during early (day ∼30-40) pregnancy the expression was highest as compared to estrous cycle.
authors.affiliation: a Jacob School of Biotechnology and Bioengineering, Department of Biological Sciences , Sam Higginbottom Institute of Agriculture, Technology & Sciences , Allahabad , India.
authors.forename: Rohit
authors.initials: R
authors.lastname: Kumar
authors.forename: P W
authors.initials: PW
authors.lastname: Ramteke
authors.forename: Sanjeev Kumar
authors.initials: SK
authors.lastname: Sharma
authors.forename: Abhijit
authors.initials: A
authors.lastname: Mitra
mesh_terms: D000449:Aldehyde Reductase / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004717:Endometrium / Q000378:metabolism*; D030762:Estrous Cycle / Q000235:genetics / Q000378:metabolism; D019143:Evolution, Molecular; D005260:Female; D006041:Goats / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D012333:RNA, Messenger; D000449:Aldehyde Reductase
keywords: AKR1B5; Estrous cycle; Molecular characterization; PGF2α; mRNA expression