title: Nonlytic viral spread enhanced by autophagy components.
abstract: The cell-to-cell spread of cytoplasmic constituents such as nonenveloped viruses and aggregated proteins is usually thought to require cell lysis. However, mechanisms of unconventional secretion have been described that bypass the secretory pathway for the extracellular delivery of cytoplasmic molecules. Components of the autophagy pathway, an intracellular recycling process, have been shown to play a role in the unconventional secretion of cytoplasmic signaling proteins. Poliovirus is a lytic virus, although a few examples of apparently nonlytic spread have been documented. Real demonstration of nonlytic spread for poliovirus or any other cytoplasmic constituent thought to exit cells via unconventional secretion requires demonstration that a small amount of cell lysis in the cellular population is not responsible for the release of cytosolic material. Here, we use quantitative time-lapse microscopy to show the spread of infectious cytoplasmic material between cells in the absence of lysis. siRNA-mediated depletion of autophagy protein LC3 reduced nonlytic intercellular viral transfer. Conversely, pharmacological stimulation of the autophagy pathway caused more rapid viral spread in tissue culture and greater pathogenicity in mice. Thus, the unconventional secretion of infectious material in the absence of cell lysis is enabled by components of the autophagy pathway. It is likely that other nonenveloped viruses also use this pathway for nonlytic intercellular spread to affect pathogenesis in infected hosts.
authors.affiliation: Departments of Microbiology and Immunology and.
authors.forename: Sara Whitney
authors.initials: SW
authors.lastname: Bird
authors.affiliation: Bioengineering, Stanford University School of Medicine, Stanford, CA 94305.
authors.forename: Nathaniel D
authors.initials: ND
authors.lastname: Maynard
authors.affiliation: Bioengineering, Stanford University School of Medicine, Stanford, CA 94305.
authors.forename: Markus W
authors.initials: MW
authors.lastname: Covert
authors.affiliation: Departments of Microbiology and Immunology and karlak@stanford.edu.
authors.forename: Karla
authors.initials: K
authors.lastname: Kirkegaard
mesh_terms: D000818:Animals; D001343:Autophagy; D045744:Cell Line, Tumor; D002470:Cell Survival; D006801:Humans; D021621:Imaging, Three-Dimensional; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D011051:Poliomyelitis / Q000473:pathology / Q000821:virology; D017955:Poliovirus / Q000502:physiology*; D059010:Single-Cell Analysis; D046509:Tissue Culture Techniques
chemical_list: D008869:Microtubule-Associated Proteins
keywords: live imaging; virus spread