title: Expression and localization of somatostatin receptor types 3, 4 and 5 in the wild-type, SSTR1 and SSTR1/SSTR2 knockout mouse cochlea.
abstract: Somatostatin (SST) is a peptide hormone that exerts inhibitory effects mediated through binding to specific cell surface G protein-coupled receptors, of which five distinct subtypes (SSTR1-SSTR5) have been characterized. Our study performed on mouse cochlear hair cells shows the expression and localization of the three receptors (SSTR3-SSTR5) in wild-type (WT), single-knockout (SSTR1 KO) and double-knockout SSTR1/SSTR2 (DKO) mice. Similar SSTRs expression were observed in the inner hair cells (IHC), outer hair cells (OHC) and supporting cells of cultivated P7 mouse organ of Corti (OC) explants as well as in cultivated cochlear neuroepithelial supporting cells (NEsc). We found differences in the expression of SSTR3-5 in WT, SSTR1 KO and DKO mouse cochlea, which might be explained as a compensatory effect in the cochlea after the loss of SSTR1 and/or SSTR2.
authors.affiliation: Department of Biomedicine, University Hospital Basel, Petersgraben 4, 4031, Basel, Switzerland, vesna.radojevic@unibas.ch.
authors.forename: Vesna
authors.initials: V
authors.lastname: Radojevic
authors.forename: Daniel
authors.initials: D
authors.lastname: Bodmer
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D003051:Cochlea / Q000196:embryology / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D046569:Neuroepithelial Cells / Q000378:metabolism; D009925:Organ of Corti / Q000378:metabolism; D017481:Receptors, Somatostatin / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism*
chemical_list: D017481:Receptors, Somatostatin; C103631:somatostatin receptor 3; C098681:somatostatin receptor subtype-4; C101130:somatostatin receptor type 1; C094573:somatostatin receptor 5; C093733:somatostatin receptor 2