title: Aqueous Extract of Chrysanthemum morifolium ( Jú Huā) Enhances the Antimelanogenic and Antioxidative Activities of the Mixture of Soy Peptide and Collagen Peptide.
abstract: The possible synergistic effect between the aqueous extract of Chrysanthemum morifolium ( Jú Huā) (AECM) and the peptide mixture (PM) containing soy peptide and collagen peptide was investigated in an ultraviolet (UV) irradiation-induced skin damage mouse model. The irradiated mice were treated with the PM or PM + AECM (containing PM and AECM), respectively. Both PM and PM + AECM groups displayed an apparent photoprotective effect on the UV-irradiated skin damage of mice. Histological evaluation demonstrated that the epidermal hyperplasia and melanocytes in the basal epidermal layer of the UV-irradiated skin in mice decreased when treated with either PM or PM + AECM. Further study showed that soy peptide, collagen peptide, and AECM also inhibited the activities of mushroom tyrosinase with IC50 values of 82.3, 28.2, and 1.6 μg/ml, respectively. Additionally, PM + AECM reduced melanogenesis by 46.2% at the concentration of 10 mg/ml in B16 mouse melanoma cells. Meanwhile, the UV-induced increase of antioxidative indicators, including glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and malondialdehyde (MDA), was reduced significantly after treatment with 1.83 g/kg/dbw of PM + AECM. This evidence supported the synergistic antioxidative effect of AECM with PM. These results demonstrated that oral intake of PM and AECM had synergistic antimelanogenic and antioxidative effects in UV-irradiated mice.
authors.affiliation: Amway (China) Research and Development Center, Shanghai, China.
authors.forename: Min
authors.initials: M
authors.lastname: Gui
authors.affiliation: Amway (China) Research and Development Center, Shanghai, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Du
authors.affiliation: State Key Laboratory of New Drug Evaluation in Guangzhou, Guangzhou Institute of Pharmaceutical Industry, Guangzhou, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Guo
authors.affiliation: State Key Laboratory of New Drug Evaluation in Guangzhou, Guangzhou Institute of Pharmaceutical Industry, Guangzhou, China.
authors.forename: Baiquan
authors.initials: B
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of New Drug Evaluation in Guangzhou, Guangzhou Institute of Pharmaceutical Industry, Guangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.affiliation: Amway (China) Research and Development Center, Shanghai, China.
authors.forename: Minjie
authors.initials: M
authors.lastname: Li
keywords: Antimelanogenic; Antioxidant; Chrysanthemum morifolium; Collagen peptide; Soy peptide