title: Simultaneous determination of trifolirhizin, (-)-maackiain, (-)-sophoranone, and 2-(2,4-dihydroxyphenyl)-5,6-methylenedioxybenzofuran from Sophora tonkinensis in rat plasma by liquid chromatography with tandem mass spectrometry and its application to a pharmacokinetic study.
abstract: A new liquid chromatography with tandem mass spectrometry method was developed and validated for the simultaneous determination of trifolirhizin, (-)-maackiain, (-)-sophoranone, and 2-(2,4-dihydroxyphenyl)-5,6-methylenedioxybenzofuran from Sophora tonkinensis in rat plasma using chlorpropamide as an internal standard. Plasma samples (50 μL) were prepared using a simple deproteinization procedure with 150 μL of acetonitrile containing 100 ng/mL of chlorpropamide. Chromatographic separation was carried out on an Acclaim RSLC120 C18 column (2.1 × 100 mm, 2.2 μm) using a gradient elution consisting of 7.5 mM ammonium acetate and acetonitrile containing 0.1% formic acid (0.4 mL/min flow rate, 7.0 min total run time). The detection and quantitation of all analytes were performed in selected reaction monitoring mode under both positive and negative electrospray ionization. This assay was linear over concentration ranges of 50-5000 ng/mL (trifolirhizin), 25-2500 ng/mL ((-)-maackiain), 5-250 ng/mL ((-)-sophoranone), and 1-250 ng/mL 2-(2,4-dihydroxyphenyl)-5,6-methylenedioxybenzofuran) with a lower limit of quantification of 50, 25, 5, and 1 ng/mL for trifolirhizin, (-)-maackiain, (-)-sophoranone, and 2-(2,4-dihydroxyphenyl)-5,6-methylenedioxybenzofuran, respectively. All the validation data, including the specificity, precision, accuracy, recovery, and stability conformed to the acceptance requirements. The results indicated that the developed method is sufficiently reliable for the pharmacokinetic study of the analytes following oral administration of Sophora tonkinensis extract in rats.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Korea.
authors.forename: Hunseung
authors.initials: H
authors.lastname: Yoo
authors.forename: Keun Ho
authors.initials: KH
authors.lastname: Ryu
authors.forename: Soo Kyung
authors.initials: SK
authors.lastname: Bae
authors.forename: Jinwoong
authors.initials: J
authors.lastname: Kim
mesh_terms: D000085:Acetates / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D052117:Benzodioxoles / Q000737:chemistry*; D001572:Benzofurans / Q000032:analysis* / Q000737:chemistry*; D001774:Blood Chemical Analysis; D002138:Calibration; D002853:Chromatography, Liquid; D005419:Flavonoids / Q000032:analysis*; D005561:Formates / Q000737:chemistry; D005960:Glucosides / Q000032:analysis*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000032:analysis*; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D010936:Plant Extracts / Q000737:chemistry*; D036343:Pterocarpans / Q000032:analysis*; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D029909:Sophora / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D000094162:Phytoalexins
chemical_list: D000085:Acetates; D000097:Acetonitriles; D052117:Benzodioxoles; D001572:Benzofurans; D005419:Flavonoids; D005561:Formates; D005960:Glucosides; D006576:Heterocyclic Compounds, 4 or More Rings; D010936:Plant Extracts; D036343:Pterocarpans; C461819:sophoranone; C526230:trifolirhizin; C030544:formic acid; C018824:ammonium acetate; C001449:inermin; D000094162:Phytoalexins; C032159:acetonitrile
keywords: Maackiain; Rat plasma; Sophora tonkinensis; Sophoranone; Trifolirhizin