title: Functional Characterization of Human Stem Cell-Derived Cardiomyocytes.
abstract: Cardiac toxicity is a leading contributor to late-stage attrition in the drug discovery process and to withdrawal of approved from the market. In vitro assays that enable earlier and more accurate testing for cardiac risk provide early stage predictive indicators that aid in mitigating risk. Human cardiomyocytes, the most relevant subjects for early stage testing, are severely limited in supply. But human stem cell-derived cardiomyocytes (SC-hCM) are readily available from commercial sources and are increasingly used in academic research, drug discovery and safety pharmacology. As a result, SC-hCM electrophysiology has become a valuable tool to assess cardiac risk associated with drugs. This unit describes techniques for recording individual currents carried by sodium, calcium and potassium ions, as well as single cell action potentials, and impedance recordings from contracting syncytia of thousands of interconnected cells.
authors.affiliation: ChanTest Corporation, Cleveland, Ohio USA.
authors.forename: Glenn E
authors.initials: GE
authors.lastname: Kirsch
authors.affiliation: ChanTest Corporation, Cleveland, Ohio USA.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Obejero-Paz
authors.affiliation: ChanTest Corporation, Cleveland, Ohio USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Bruening-Wright
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology; D055724:Electrophysiological Phenomena / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology; D009200:Myocardial Contraction / Q000187:drug effects / Q000502:physiology; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques; D013234:Stem Cells / Q000502:physiology*
chemical_list: D015220:Calcium Channels; D007473:Ion Channels
keywords: Stem cell-derived cardiomyocytes; electrophysiology; impedance