title: C13orf18 and C1orf166 (MULAN) DNA genes methylation are not associated with cervical cancer and precancerous lesions of human papillomavirus genotypes in Iranian women.
abstract: BACKGROUND
  Nowadays, molecular biomarkers have critical roles for cancer diagnosis and prognosis in clinical laboratories. Human papillomaviruses are the main agents for etiology of cervical carcinoma. The present survey was conducted to evaluate the genes methylation in cervical cancer and precancerous lesions involvement with HPV genotypes.
  
  
  METHODS
  C13orf18 and C1orf166 (MUL1 or Mulan) DNA methylation as potential biomarkers and risk factors was investigated in 112 liquid based cytology and Formalin-Fixed Paraffin- Embedded tissue specimens in Iranian females with cervical intraepithelial neoplasia and dysplasia.
  
  
  RESULTS
  In this survey, HPV18 (61.6%) and HPV16 (42.9%) proved to be the most common HPV genotypes identified by In-House Multiplex Real Time PCR. There were no significant relationship between HPV positivity and the methylated DNA genes mentioned above (p>0.05).
  
  
  CONCLUSIONS
  Our MethyLight data demonstrated that these genes could not be considered as specific, sensitive and suitable prognostic biomarkers in cervical dysplasia related HPV. It is suggested that further studies with more patients should be done on candidate methylated markers in different countries in order to plan for cervical cancer prevention.
authors.affiliation: Department of Molecular Medicine, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran E-mail : sohrabi@razi.tums.ac.ir, modaresi@tums.ac.ir.
authors.forename: Amir
authors.initials: A
authors.lastname: Sohrabi
authors.forename: Siamak
authors.initials: S
authors.lastname: Mirab-Samiee
authors.forename: Marjan
authors.initials: M
authors.lastname: Rahnamaye-Farzami
authors.forename: Mitra
authors.initials: M
authors.lastname: Rafizadeh
authors.forename: Setareh
authors.initials: S
authors.lastname: Akhavan
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hashemi-Bahremani
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Modarresl
mesh_terms: D019175:DNA Methylation / Q000235:genetics; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics; D052161:Human papillomavirus 18 / Q000235:genetics; D006801:Humans; D007492:Iran; D030361:Papillomavirus Infections / Q000235:genetics; D016612:Paraffin Embedding; D011230:Precancerous Conditions / Q000235:genetics / Q000821:virology; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*; D002578:Uterine Cervical Dysplasia / Q000235:genetics* / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology
chemical_list: C585115:RUBCNL protein, human; D025521:Tumor Suppressor Proteins; C529067:MUL1 protein, human; D044767:Ubiquitin-Protein Ligases