title: Genome wide association study of cholesterol and poly- and monounsaturated fatty acids, protein, and mineral content of beef from crossbred cattle.
abstract: The objectives were to determine the variation explained by the BovineSNP50v2 BeadChip for cholesterol (CH), polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA), protein, and minerals in beef cattle, and to identify chromosomal regions that harbor major allelic variants underlying the variation of these traits. Crossbred steers and heifers (n=236) segregating at the inactive myostatin allele on BTA2 were harvested and steaks were sampled from the M. semitendinosus and the M. longissimus thoracis et lumborum for nutrient analysis. A Bayes C algorithm was employed in genome-wide association analysis. The resulting posterior heritability (SD) estimates ranged from 0.43 (0.10) to 0.71 (0.08) for lipid traits and 0.05 (0.08) to 0.75 (0.06) for mineral traits. Across cuts, correlations between genomic estimated breeding values (GEBV) were similar for CH, MUFA and PUFA. The top 0.5% 1-Mb windows for all traits explained up to 9.93% of the SNP variance. Slight differences did exist between cuts and between different measurement scales of fatty acids.
authors.affiliation: Animal Science Department, University of Nebraska, Lincoln, NE 68583, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Ahlberg
authors.affiliation: Animal Science Department, University of Nebraska, Lincoln, NE 68583, USA.
authors.forename: L N
authors.initials: LN
authors.lastname: Schiermiester
authors.affiliation: Animal Science Department, University of Nebraska, Lincoln, NE 68583, USA.
authors.forename: J
authors.initials: J
authors.lastname: T
authors.affiliation: Animal Science Department, University of Nebraska, Lincoln, NE 68583, USA.
authors.lastname: Howard
authors.affiliation: Animal Science Department, University of Nebraska, Lincoln, NE 68583, USA.
authors.forename: C R
authors.initials: CR
authors.lastname: Calkins
authors.affiliation: Animal Science Department, University of Nebraska, Lincoln, NE 68583, USA. Electronic address: mspangler2@unl.edu.
authors.forename: M L
authors.initials: ML
authors.lastname: Spangler
mesh_terms: D000818:Animals; D002417:Cattle; D002784:Cholesterol / Q000032:analysis*; D004044:Dietary Proteins / Q000032:analysis*; D005229:Fatty Acids, Monounsaturated / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D005260:Female; D055106:Genome-Wide Association Study / Q000379:methods*; D008297:Male; D008460:Meat / Q000032:analysis*; D008903:Minerals / Q000032:analysis*; D018482:Muscle, Skeletal
chemical_list: D004044:Dietary Proteins; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; D008903:Minerals; D002784:Cholesterol
keywords: Beef; Genome wide association; Nutrient profile