title: Antimicrobial activity of engineered shrimp ovarian peritrophin fragments from Fenneropenaeus merguiensis.
abstract: Shrimp ovarian peritrophin (SOP), a major protein in jelly layer and cortical rods, plays a role in egg protection after spawning. Previous study, sequence of SOP gene from Fenneropenaeus merguiensis (Fm-SOP) was composed of domain A and domain B. The SOP domain A contains amino acid sequences between 1-80 of Fm-SOP. The domain A had six conserved cysteines which have been found in many antimicrobial peptides. The molecular weight of purified rSOP-A protein was about 9 kDa. The SOP domain B contains amino acid sequences 81-329 of Fm-SOP while SOP-B1 was amino acid sequence 182-275 of Fm-SOP. The molecular weight of purified rHis-SOP-B and rHis-SOP-B1 protein were about 38.5 and 18.0 kDa, respectively. Antimicrobial activities of rSOP-A, rHis-SOP-B and rHis-SOP-B1 protein were investigated by liquid growth inhibition assay. Minimal Inhibition Concentration (MIC) of rSOP-A against Staphylococcus aureus, Escherichia coli, Vibrio harveyi, Candida albicans and Fusarium oxysporum were 35, 280, 280, 570 and 15 µg/mL, respectively. The MIC of rHis-SOP-B against S. aureus, V. harveyi and F. oxysporum were 30, 270 and 500 µg/mL, respectively. And the MIC of rHis-SOP-B1 against S. aureus, V. harveyi and F. oxysporum were 20, 470 and 250 µg/mL, respectively. The rHis-SOP-B and rHis-SOP-B1 (1000 µg/mL) did not show antimicrobial activity against E. coli and C. albicans. Three purified proteins were able to agglutinate V. harveyi in vitro, displayed a chitinase activity and proteinase inhibition. In addition the stability of the proteins was tested and found decrease antimicrobial activity after incubation at 50 °C for 5 h.
authors.forename: Sujunya
authors.initials: S
authors.lastname: Anuchan
authors.forename: Panchalika
authors.initials: P
authors.lastname: Deachamag
authors.forename: Nareerat
authors.initials: N
authors.lastname: Siammai
authors.forename: Souwalak
authors.initials: S
authors.lastname: Phongpaichit
authors.affiliation: Center for Genomics and Bioinformatics Research, Department of Molecular Biotechnology and Bioinformatics, Department of Microbiology and Natural Product Research Center of Excellence, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla, 90112, Thailand. wilaiwan58@hotmail.com.
authors.forename: Wilaiwan
authors.initials: W
authors.lastname: Chotigeat
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D008562:Membrane Glycoproteins / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D033561:Penaeidae / Q000737:chemistry / Q000235:genetics*; D015202:Protein Engineering; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D060829:Arthropod Proteins; D008562:Membrane Glycoproteins; C000599832:ovarian peritrophin, Fenneropenaeus chinensis