title: MicroRNA-1246 enhances migration and invasion through CADM1 in hepatocellular carcinoma.
abstract: BACKGROUND
  The aberrant expression of microRNAs has been demonstrated to play a crucial role in the initiation and progression of hepatocarcinoma. miR-1246 expression in High invasive ability cell line than significantly higher than that in low invasive ability cell line.
  
  
  METHODS
  Transwell chambers (8-uM pore size; Costar) were used in the in vitro migration and invison anssay. Dual luciferase reporter gene construct and Dual luciferase reporter assay to identify the target of miR-1246. CADM1 expression was evaluated by immunohistochemistric staining. The clinical manifestations, treatments and survival were collected for statistical analysis.
  
  
  RESULTS
  Inhibition of miR-1246 effectively reduced migration and invasion of hepatocellular carcinoma cell lines. Bioinformatics and luciferase reporter assay revealed that miR-1246 specifically targeted the 3'-UTR of Cell adhesion molecule 1 and regulated its expression. Down-regulation of CADM1 enhanced migration and invasion of HCC cell lines. Furthermore, in tumor tissues obtained from liver cancer patients, the expression of miR-1246 was negatively correlated with CADM1 and the high expression of miR-1246 combined with low expression of CADM1 might serve as a risk factor for stage1 liver cancer patients.
  
  
  CONCLUSIONS
  Our study showed that miR-1246, by down-regulation CADM1, enhances migration and invasion in HCC cells.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Sun
authors.forename: Changting
authors.initials: C
authors.lastname: Meng
authors.forename: Shihua
authors.initials: S
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Zhou
authors.forename: Mei
authors.initials: M
authors.lastname: Guan
authors.forename: Chunmei
authors.initials: C
authors.lastname: Bai
authors.forename: Shan
authors.initials: S
authors.lastname: Lu
authors.affiliation: Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, People's Republic of China. hanqinhanqin@126.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Han
authors.forename: Robert Chunhua
authors.initials: RC
authors.lastname: Zhao
mesh_terms: D020413:3' Untranslated Regions; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D012307:Risk Factors; D016019:Survival Analysis
chemical_list: D020413:3' Untranslated Regions; C403514:CADM1 protein, human; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; C577700:MIRN1246 microRNA, human; D035683:MicroRNAs