title: Presentation of fibronectin fragments using affinity protein interactions for enhanced retention and function.
abstract: We present a protein immobilization system, based on the Src Homology 3 (SH3) affinity domain, allowing for a transient interaction between a fibronectin ligand and a biomaterial surface. This strategy leads to enhanced retention of the fibronectin fragment over adsorbed fibronectin, and increased cellular proliferation and motility over either covalently immobilized or adsorbed fibronectin. The results indicate that intermediate affinity protein immobilization could provide benefits for tissue engineering beyond the traditional immobilization techniques, adsorption or covalent attachment.
authors.affiliation: School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
authors.forename: Bradley R
authors.initials: BR
authors.lastname: Silverman
authors.affiliation: School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA. Electronic address: julie.champion@chbe.gatech.edu.
authors.forename: Julie A
authors.initials: JA
authors.lastname: Champion
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry*; D000327:Adsorption; D000818:Animals; D001665:Binding Sites; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D005353:Fibronectins / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D010750:Phosphoproteins / Q000737:chemistry*; D011485:Protein Binding; D023822:Tissue Engineering / Q000379:methods*; D018909:src Homology Domains
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D010750:Phosphoproteins; C092947:SLP-76 signal Transducing adaptor proteins
keywords: Affinity; Cell motility; Engineered proteins; Fibronectin; Wound healing