title: Cell-derived microparticles after exercise in individuals with G6PD Viangchan.
abstract: Glucose-6-phospate dehydrogenase (G6PD) deficient cells are sensitive to oxidative damage leading to the formation of microparticles (MPs). Therefore, we examined the concentration of MPs and changes in the antioxidant balance after an acute strenuous exercise (SEx) and moderate-intensity exercise (MEx). Eighteen healthy females (18-24 years) with G6PD normal and eighteen age-matched females with G6PD Viangchan (871G>A) were tested by running on a treadmill at their maximal oxygen uptake for SEx and at 75% of their maximal heart rate for MEx. It was found that SEx triggered the release of total microparticles (TTMPs) above baseline levels and remained significantly higher 45 minutes after the exercise in G6PD normal individuals. However, SEx-induced increase in TTMPs was significantly higher in G6PD Viangchan as compared to G6PD normal. In contrast, MEx did not to alter the release of TTMPs in both G6PD normal and Viangchan. Moreover, TTMPs concentrations were inversely correlated with G6PD activity (r =-0.82, P <  0.05) but positively correlated with MDA concentrations (r = 0.74, P <  0.05). Using cell specific antibodies, we determined that MPs were mainly derived from platelets and erythrocytes. Altogether, the present study indicates that G6PD Viangchan may participate in MEx without higher MPs concentration and oxidative stress compared with G6PD normal.
authors.affiliation: Molecular Hematology Research Unit, Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Makamas
authors.initials: M
authors.lastname: Chanda
authors.affiliation: Molecular Hematology Research Unit, Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Duangdao
authors.initials: D
authors.lastname: Nantakomol
authors.affiliation: Faculty of Sports Science, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Daroonwan
authors.initials: D
authors.lastname: Suksom
authors.affiliation: Molecular Hematology Research Unit, Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Attakorn
authors.initials: A
authors.lastname: Palasuwan
mesh_terms: D000293:Adolescent; D000975:Antioxidants; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D015444:Exercise / Q000502:physiology / Q000523:psychology*; D005260:Female; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000097:blood*; D006801:Humans; D018384:Oxidative Stress; D055815:Young Adult
chemical_list: D000975:Antioxidants
keywords: G6PD; antioxidant status; oxidative stress