title: The characteristics of a novel heterotrophic nitrifying and aerobic denitrifying bacterium, Acinetobacter junii YB.
abstract: A novel heterotrophic nitrifying bacterium was isolated from activated sludge and was identified as Acinetobacter junii YB. The strain exhibited efficient heterotrophic nitrification-aerobic denitrification ability at a broad range of ammonium loads and had the capability to utilize hydroxylamine, nitrite and nitrate as a sole nitrogen source. Based on the nitrogen removal and enzyme assay, the nitrogen removal pathway was speculated to be achieved through heterotrophic nitrification coupled with aerobic denitrification. In addition, single-factor experiments showed that efficient heterotrophic nitrification and growth of strain YB occurred with succinate as the carbon source, pH 7.5, 37 °C, and high C/N ratio and dissolved oxygen. Furthermore, the new isolate showed capacities for aggregation and hydrophobicity. Regular variations of the flocculating ability and relative hydrophobicity were observed during the whole cultivation. The ability to perform heterotrophic nitrification-aerobic denitrification and cell aggregation demonstrated the great potential of the strain YB for future applications.
authors.affiliation: Key Lab of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an 710055, China. Electronic address: ryx@xauat.edu.cn.
authors.forename: Yong-Xiang
authors.initials: YX
authors.lastname: Ren
authors.affiliation: Key Lab of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Lab of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Liang
mesh_terms: D000150:Acinetobacter / Q000235:genetics* / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D000704:Analysis of Variance; D002244:Carbon / Q000378:metabolism; D002449:Cell Aggregation / Q000502:physiology; D017931:DNA Primers / Q000235:genetics; D005427:Flocculation; D052836:Heterotrophic Processes / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D058465:Nitrification / Q000502:physiology*; D009584:Nitrogen / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet; D019802:Succinic Acid / Q000378:metabolism
chemical_list: D017931:DNA Primers; D012722:Sewage; D002244:Carbon; D019802:Succinic Acid; D009584:Nitrogen
keywords: Acinetobacter junii YB; Cell aggregation; Heterotrophic nitrification–aerobic denitrification; Nitrogen removal pathway