title: Label-free mass spectrometric profiling of urinary proteins and metabolites from paediatric idiopathic nephrotic syndrome.
abstract: Idiopathic nephrotic syndrome (INS) is caused by renal diseases that increase the permeability of the glomerular filtration barrier without evidence of a specific systemic cause. The aim of the present work was to reveal inherent molecular features of INS in children using combined urinary proteomics and metabolomics profiling. In this study, label-free mass spectrometric analysis of urinary proteins and small molecule metabolites was carried out in 12 patients with INS versus 12 sex- and age-matched control subjects with normal renal function. Integration and biological interpretation of obtained results were carried out by Ingenuity IPA software. Validation of obtained proteomics data was carried out by Western blot method. Proteomics data have been deposited to the ProteomeXchange Consortium with the data set identifier PXD000765. This study indicates for the first time that paediatric INS is associated with up-regulation of afamin, hydroxyphenylacetate and uridine, and concomitant down-regulation in glutamine and phenylalanine levels, and many of these molecular species were previously shown to be involved in oxidative stress. Further studies in larger patient population are underway to investigate the role of oxidative stress in renal injury in paediatric INS.
authors.affiliation: Department of Biotechnology, University of Rijeka, Rijeka, Croatia.
authors.forename: Mirela
authors.initials: M
authors.lastname: Sedic
authors.affiliation: Waters Corporation, MS Technologies, Manchester, United Kingdom.
authors.forename: Lee A
authors.initials: LA
authors.lastname: Gethings
authors.affiliation: Waters Corporation, MS Technologies, Manchester, United Kingdom.
authors.forename: Johannes P C
authors.initials: JP
authors.lastname: Vissers
authors.affiliation: Waters Corporation, Milford, MA, USA.
authors.forename: John P
authors.initials: JP
authors.lastname: Shockcor
authors.affiliation: Waters Corporation, Milford, MA, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: McDonald
authors.affiliation: Department of Functional and Comparative Genomics, Institute of Integrative Biology, University of Liverpool, United Kingdom.
authors.forename: Olga
authors.initials: O
authors.lastname: Vasieva
authors.affiliation: Division of Nephrology, Department of Pediatrics, Zagreb University Hospital Centre, Zagreb, Croatia.
authors.forename: Maja
authors.initials: M
authors.lastname: Lemac
authors.affiliation: Waters Corporation, MS Technologies, Manchester, United Kingdom.
authors.forename: James I
authors.initials: JI
authors.lastname: Langridge
authors.affiliation: Division of Nephrology, Department of Pediatrics, Zagreb University Hospital Centre, Zagreb, Croatia.
authors.forename: Danica
authors.initials: D
authors.lastname: Batinić
authors.affiliation: Department of Biotechnology, University of Rijeka, Rijeka, Croatia. Electronic address: sandrakp@biotech.uniri.hr.
authors.forename: Sandra Kraljević
authors.initials: SK
authors.lastname: Pavelić
mesh_terms: D015153:Blotting, Western; D002648:Child; D005260:Female; D006801:Humans; D007674:Kidney Diseases / Q000378:metabolism* / Q000652:urine; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics; D011507:Proteinuria / Q000652:urine*
keywords: Afamin; Metabolomics; Oxidative stress; Pediatric nephrotic syndrome; Proteomics; Urine