title: Evaluation of microsatellite markers for populations studies and forensic identification of African lions (Panthera leo).
abstract: The South African lion (Panthera leo) population is highly fragmented. One-third of its wild lions occur in small (<1000 km(2)) reserves. These lions were reintroduced from other areas of the species' historical range. Management practices on these reserves have not prioritized genetic provenance or heterozygosity. These trends potentially constrain the conservation value of these lions. To ensure the best management and long-term survival of these subpopulations as a viable collective population, the provenance and current genetic diversity must be described. Concurrently, poaching of lions to supply a growing market for lion bones in Asia may become a serious conservation challenge in the future. Having a standardized, validated method for matching confiscated lion parts with carcasses will be a key tool in investigating these crimes. We evaluated 28 microsatellites in the African lion using samples from 18 small reserves and 1 captive facility in South Africa, two conservancies in Zimbabwe, and Kruger National and Kgalagadi Transfrontier Parks to determine the loci most suited for population management and forensic genetic applications. Twelve microsatellite loci with a match probability of 1.1×10(-5) between siblings were identified for forensics. A further 10 could be added for population genetics studies.
authors.affiliation: From the Department of Nature Conservation, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa (Miller); the Veterinary Genetics Laboratory, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa (Miller and Harper); the Molecular Ecology and Evolution Programme, Department of Genetics, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa (Bloomer); the Veterinary Wildlife Services, South African National Parks, Private Bag X402, Skukuza 1350, South Africa (Hofmeyr); the Department of Nature Conservation, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa (Funston); and the Lion Program, Panthera, New York, NY (Funston). dangerousfrizbee@gmail.com.
authors.forename: Susan M
authors.initials: SM
authors.lastname: Miller
authors.affiliation: From the Department of Nature Conservation, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa (Miller); the Veterinary Genetics Laboratory, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa (Miller and Harper); the Molecular Ecology and Evolution Programme, Department of Genetics, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa (Bloomer); the Veterinary Wildlife Services, South African National Parks, Private Bag X402, Skukuza 1350, South Africa (Hofmeyr); the Department of Nature Conservation, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa (Funston); and the Lion Program, Panthera, New York, NY (Funston).
authors.forename: Cindy K
authors.initials: CK
authors.lastname: Harper
authors.affiliation: From the Department of Nature Conservation, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa (Miller); the Veterinary Genetics Laboratory, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa (Miller and Harper); the Molecular Ecology and Evolution Programme, Department of Genetics, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa (Bloomer); the Veterinary Wildlife Services, South African National Parks, Private Bag X402, Skukuza 1350, South Africa (Hofmeyr); the Department of Nature Conservation, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa (Funston); and the Lion Program, Panthera, New York, NY (Funston).
authors.forename: Paulette
authors.initials: P
authors.lastname: Bloomer
authors.affiliation: From the Department of Nature Conservation, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa (Miller); the Veterinary Genetics Laboratory, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa (Miller and Harper); the Molecular Ecology and Evolution Programme, Department of Genetics, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa (Bloomer); the Veterinary Wildlife Services, South African National Parks, Private Bag X402, Skukuza 1350, South Africa (Hofmeyr); the Department of Nature Conservation, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa (Funston); and the Lion Program, Panthera, New York, NY (Funston).
authors.forename: Jennifer
authors.initials: J
authors.lastname: Hofmeyr
authors.affiliation: From the Department of Nature Conservation, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa (Miller); the Veterinary Genetics Laboratory, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa (Miller and Harper); the Molecular Ecology and Evolution Programme, Department of Genetics, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa (Bloomer); the Veterinary Wildlife Services, South African National Parks, Private Bag X402, Skukuza 1350, South Africa (Hofmeyr); the Department of Nature Conservation, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa (Funston); and the Lion Program, Panthera, New York, NY (Funston).
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Funston
mesh_terms: D000483:Alleles; D000818:Animals; D003247:Conservation of Natural Resources / Q000379:methods*; D004247:DNA / Q000302:isolation & purification; D005260:Female; D053803:Forensic Genetics; D005828:Genetics, Population / Q000379:methods*; D008045:Lions / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats; D017422:Sequence Analysis, DNA; D013019:South Africa
chemical_list: D004247:DNA
keywords: conservation genetic management; population genetics; wildlife forensics