title: HPV in HIV-Infected Women: Implications for Primary Prevention.
abstract: BACKGROUND
  There is growing evidence that human immunodeficiency virus (HIV)-infected women might have a different human papillomavirus (HPV) type distribution in cervical dysplasia specimens as compared to the general population. This has implications for primary prevention.
  
  
  OBJECTIVE
  We aimed to obtain preliminary data on the HPV genotypes prevalent in histological samples of HIV-infected women with cervical intraepithelial neoplasia (CIN) 3/CIS of the cervix in Miami, FL, USA.
  
  
  METHODS
  Retrospective data were collected on HIV-infected women referred to the University of Miami-Jackson Memorial Hospital colposcopy clinic between years 2000 and 2008. The histology slides of CIN 3/CIS biopsies underwent pathological review and sections were cut from these archived specimens for HPV DNA extraction. HPV genotyping was then performed using the GeneSquare™ HPV genotyping assay. We report on our first set of 23 samples.
  
  
  RESULTS
  Eight high-risk HPV types were detected. Types in decreasing order of frequency were 16, 35, 45, 52, 59, 31, 58, and 56. Most cases had multiple infections. HPV type 16 was the most common (45%) followed by HPV-35 and -45 with equal frequency (40%). No samples contained HPV-18.
  
  
  CONCLUSIONS
  Our preliminary results suggest that cervical dysplasia specimens of HIV-infected women more likely (55%) contain non-16 and -18 high-risk HPV types. We show that this held true for histologically confirmed severe dysplasia and carcinoma-in situ. Epidemiological studies guide vaccine development, therefore HPV type prevalence in CIS and invasive cervical cancer among HIV-infected women should be more rigorously explored to ensure that this highly vulnerable population receives appropriate primary prevention.
authors.affiliation: Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Miami Miller School of Medicine , Miami, FL , USA ; Department of Public Health Sciences, University of Miami Miller School of Medicine , Miami, FL , USA ; Division of Gynecologic Oncology, University of Florida Health Cancer Center - Orlando Health , Orlando, FL , USA.
authors.forename: Nathalie Dauphin
authors.initials: ND
authors.lastname: McKenzie
authors.affiliation: Department of Public Health Sciences, University of Miami Miller School of Medicine , Miami, FL , USA ; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine , Miami, FL , USA.
authors.forename: Erin N
authors.initials: EN
authors.lastname: Kobetz
authors.affiliation: Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine , Miami, FL , USA ; Department of Pathology, University of Miami Miller School of Medicine , Miami, FL , USA.
authors.forename: Parvin
authors.initials: P
authors.lastname: Ganjei-Azar
authors.affiliation: Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine , Miami, FL , USA ; Division of Infectious Diseases, Department of Medicine, University of Miami Miller School of Medicine , Miami, FL , USA.
authors.forename: Isabella
authors.initials: I
authors.lastname: Rosa-Cunha
authors.affiliation: Division of Women's Health Research, Department of Obstetrics and Gynecology, University of Miami Miller School of Medicine , Miami, FL , USA.
authors.forename: JoNell E
authors.initials: JE
authors.lastname: Potter
authors.affiliation: Bio-Medical Department, Kurabo Industries LTD , Osaka , Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Morishita
authors.affiliation: Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Miami Miller School of Medicine , Miami, FL , USA.
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Lucci
authors.affiliation: Oncogenomics Core Facility, Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine , Miami, FL , USA.
authors.forename: Toumy
authors.initials: T
authors.lastname: Guettouche
authors.affiliation: Division of Hematology and Oncology, Department of Medicine, University of Miami Miller School of Medicine , Miami, FL , USA.
authors.forename: James H
authors.initials: JH
authors.lastname: Hnatyszyn
authors.affiliation: Department of Public Health Sciences, University of Miami Miller School of Medicine , Miami, FL , USA ; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine , Miami, FL , USA.
authors.forename: Tulay
authors.initials: T
authors.lastname: Koru-Sengul
keywords: CIN; HIV; HPV; cervical cancer