title: The autotetraploid fish derived from hybridization of Carassius auratus red var. (female) × Megalobrama amblycephala (male).
abstract: The establishment of the tetraploid organism is difficult but useful in genetics and breeding. In the present study, we have artificially established an autotetraploid fish line (F2-F8) derived from the distant hybridization of Carassius auratus red var. (RR, 2n = 100) (female) × Megalobrama amblycephala (BB, 2n = 48) (male). The autotetraploid line (F2-F8) possess four sets of chromosomes from red crucian carp (RRRR, 4n = 200) and produce diploid ova and diploid sperm, which maintains the formation of the autotetraploid line. The F2 of the autotetraploid fish result from the fertilization of the autodiploidy diploid eggs and diploid sperm from the females and males of F1 hybrids (RRBB, 4n = 148), which exhibit abnormal chromosome behavior during meiosis as revealed by gynogenesis and backcrossing. This is the first report concerning the establishment of an autotetraploid fish line derived from distant hybridization. The autotetraploid fish line provides an important gamete source for the production of triploids and tetraploids. The autotetraploid fish line also provides an ideal system to investigate the poorly understood mechanisms that drive diploidization in autotetraploids and to study the hybrid progenies' characteristics, including the appearance of new traits that promote a diversity of traits and facilitate adaptation.
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China.
authors.forename: Qinbo
authors.initials: Q
authors.lastname: Qin
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China.
authors.forename: Yude
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Dai
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China.
authors.forename: Fangzhou
authors.initials: F
authors.lastname: Hu
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China.
authors.forename: Kaikun
authors.initials: K
authors.lastname: Luo
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Tao
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China.
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China lsj@hunnu.edu.cn.
authors.forename: Shaojun
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D003530:Cyprinidae / Q000235:genetics* / Q000502:physiology; D004247:DNA / Q000235:genetics; D005260:Female; D006824:Hybridization, Genetic; D017404:In Situ Hybridization, Fluorescence; D059785:Karyotype; D008297:Male; D011003:Ploidies
chemical_list: D004247:DNA
keywords: allotetraploid; autotetraploid line; chromosome behavior; diploid gamete; distant hybridization