title: Adhesive interface and bond strength of endodontic sealers to root canal dentine after immersion in phosphate-buffered saline.
abstract: This study evaluated the bond strength (BS) and the adhesive interface of four endodontic sealers to root canal dentine, before, and after immersion in phosphate buffered saline (PBS) to simulate an in vivo environment. Eighty roots were instrumented using ProTaper rotatory files, under irrigation with 17% EDTA and 1% NaOCl. Posteriorly were divided into four groups (n = 20) according to the sealer used: Endofill, AH Plus, Sealapex, and MTA Fillapex. Each group was divided into two subgroups (n = 10) and stored at 37°C immersed in water for 7 days and in PBS for 60 days. From each subgroup, 1 mm thick sections were obtained. One section of each region (coronal, middle, and apical) was submitted to the push-out test and failures were observed. Twelve sections of each subgroup (four from each region) were evaluated under SEM. Three-way ANOVA evaluation for BS showed significant differences between groups and regions (P < 0.0001), but not between subgroups (P > 0.05). AH Plus had significantly higher BS than the others sealers, regardless of the analyzed subgroup (Tukey's test, P < 0.5). The most common failures were adhesive to dentine and cohesive of the sealer. The SEM evaluation (Kruskal-Wallis, Mann-Whitney) showed homogeneous adhesive interface formed and sealer tags in all groups with significant statistical differences with AH Plus, regardless of PBS immersion. AH Plus was superior to the other sealers for both BS and quality of interface formation. Immersion in PBS did not interfere on BS or adhesive interface of the sealers tested.
authors.affiliation: Department of Dentistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina, CEP 88040-900, Brazil.
authors.forename: Maybell
authors.initials: M
authors.lastname: Tedesco
authors.forename: Mara Cristina Santos
authors.initials: MC
authors.lastname: Felippe
authors.forename: Wilson Tadeu
authors.initials: WT
authors.lastname: Felippe
authors.forename: Ana Maria Hecke
authors.initials: AM
authors.lastname: Alves
authors.forename: Eduardo Antunes
authors.initials: EA
authors.lastname: Bortoluzzi
authors.forename: Cleonice Silveira
authors.initials: CS
authors.lastname: Teixeira
mesh_terms: D000268:Adhesiveness; D006801:Humans; D007180:Incisor / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D010895:Pit and Fissure Sealants / Q000737:chemistry*; D012387:Root Canal Filling Materials / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry; D013499:Surface Properties
chemical_list: D010895:Pit and Fissure Sealants; D012387:Root Canal Filling Materials; D012965:Sodium Chloride
keywords: adhesive interface; bond strength; endodontic sealers; scanning electron microscopy