title: Mass transfer analysis of growth and substance metabolism of NSCs cultured in collagen-based scaffold in vitro.
abstract: The aim of this study is to analyze the growth and substance metabolism of neural stem cells (NSCs) cultured in biological collagen-based scaffolds. Mass transfer and metabolism model of glucose, lactic acid, and dissolved oxygen (DO) were established and solved on MATLAB platform to obtain the concentration distributions of DO, glucose, and lactic acid in culture system, respectively. Calculation results showed that the DO influenced their normal growth and metabolism of NSCs mostly in the in vitro culture within collagen-based scaffolds. This study also confirmed that 2-mm thickness of collagen scaffold was capable of in vitro cultivation and growth of NSCs with an inoculating density of 1 × 10(6) cells/mL.
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian, 116024, China, Kedongsong@dlut.edu.cn.
authors.forename: Kedong
authors.initials: K
authors.lastname: Song
authors.forename: Dan
authors.initials: D
authors.lastname: Ge
authors.forename: Shui
authors.initials: S
authors.lastname: Guan
authors.forename: Chenggong
authors.initials: C
authors.lastname: Sun
authors.forename: Xuehu
authors.initials: X
authors.lastname: Ma
authors.forename: Tianqing
authors.initials: T
authors.lastname: Liu
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D019344:Lactic Acid / Q000378:metabolism; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D019344:Lactic Acid; D003094:Collagen; D005947:Glucose; D010100:Oxygen