title: Effect of topically applied sildenafil citrate on wound healing: experimental study.
abstract: Wound healing is a complex process that necessitates organization of different cell types and several signalling molecules. The aim of this study is to evaluate the effect of different concentrations of sildenafil citrate, which decreases cGMP degradation, on wound healing by secondary intention.This study was performed using 25 Sprague Dawley rats weighing 200-250 grams. 4 dorsal defects were created. Four different treatment modalities which were 1% and 5% sildenafil citrate gel prepared with carbopol, pure carbopol gel without any drug in it and 0,9% NaCl solution; were applied to each lesion of the same rat. Randomly selected five rats (25 rats in total) were sacrificed on 3rd, 5th, 7th, 10th, and 14th days; and the effect of each modality was evaluated by means of defect area measurement, histopathological examination and measurement of tissue hydroxyproline levels.Sildenafil citrate gel application decreased the defect areas in a dose independent manner starting from 3rd day and dose dependent manner after 7th day. By means of vascularization, sildenafil citrate increased vascularity starting from 3rd day. The strength of acute inflammation was superior in sildenafil groups starting from 5th day; and the amount and maturation of granulation in the wound bed, as well as the strength of chronic inflammation were superior in defects treated with sildenafil citrate as early as 7th day.
authors.affiliation: ANKARA RESEARCH AND TRAINING HOSPITAL, PLASTIC, RECONSTRUCTIVE AND AESTHETIC SURGERY CLINIC ANKARA. koraymd@yahoo.com.
authors.forename: Koray
authors.initials: K
authors.lastname: Gürsoy
authors.forename: Melike
authors.initials: M
authors.lastname: Oruç
authors.forename: Yüksel
authors.initials: Y
authors.lastname: Kankaya
authors.forename: Mustafa Gürhan
authors.initials: MG
authors.lastname: Ulusoy
authors.forename: Uğur
authors.initials: U
authors.lastname: Koçer
authors.forename: Duygu
authors.initials: D
authors.lastname: Kankaya
authors.forename: Reyhan Neslihan
authors.initials: RN
authors.lastname: Gürsoy
authors.forename: Özge
authors.initials: Ö
authors.lastname: Çevik
authors.forename: Elmas
authors.initials: E
authors.lastname: Öğüş
authors.forename: Vildan
authors.initials: V
authors.lastname: Fidanci
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000287:Administration, Topical; D000818:Animals; D003094:Collagen / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D006909:Hydroxyproline / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy; D010879:Piperazines / Q000008:administration & dosage*; D011687:Purines / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D000068677:Sildenafil Citrate; D013449:Sulfonamides / Q000008:administration & dosage*; D013997:Time Factors; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000180:Acrylic Resins; D010879:Piperazines; D011687:Purines; D013449:Sulfonamides; C006912:carboxypolymethylene; D003094:Collagen; D000068677:Sildenafil Citrate; D006909:Hydroxyproline