title: Genotoxicity effects of Flusilazole on the somatic cells of Allium cepa.
abstract: The aim of this study was to evaluate the effects of the fungicide flusilazole on somatic cells of Allium cepa. For evaluation of cytogenetic effects, root meristem cells of A. cepa were treated with 10, 20, 30 and 45 ppm (EC50 concentration) for 24, 48 and 72 h. The mitotic index and different types of chromosomal abnormalities such as bridges, stickiness and laggards were determined in both control and test groups. Acridine orange/Ethidium bromide double staining and fluorescence microscope was used to determine the stability of chromosome structure. Data obtained from staining process indicated that ratio of necrotic cells significantly increased by the flusilazole presoaking. The RAPD-PCR method was used and the higher doses treated-group (45 ppm) was more distant to the control group compare with others.
authors.affiliation: Department of Molecular Biology and Genetic, Faculty of Art & Science, Bilecik Seyh Edebali University, 11210 Bilecik, Turkey. Electronic address: dilek.unal@bilecik.edu.tr.
authors.forename: Dilek Unal
authors.initials: DU
authors.lastname: Ozakca
authors.affiliation: Department of Chemistry, Faculty of Art & Science, Bilecik Seyh Edebali University, 11210 Bilecik, Turkey. Electronic address: hulya.mercan@bilecik.edu.tr.
authors.forename: Hulya
authors.initials: H
authors.lastname: Silah
mesh_terms: D000490:Allium / Q000187:drug effects* / Q000235:genetics; D002869:Chromosome Aberrations / Q000139:chemically induced; D032461:Chromosomes, Plant / Q000187:drug effects / Q000235:genetics; D004249:DNA Damage; D018744:DNA, Plant / Q000235:genetics; D005659:Fungicides, Industrial / Q000633:toxicity*; D018519:Meristem / Q000187:drug effects* / Q000235:genetics; D008940:Mitotic Index; D009153:Mutagens / Q000633:toxicity*; D019105:Random Amplified Polymorphic DNA Technique; D012821:Silanes / Q000633:toxicity*; D014230:Triazoles / Q000633:toxicity*
chemical_list: D018744:DNA, Plant; D005659:Fungicides, Industrial; D009153:Mutagens; D012821:Silanes; D014230:Triazoles; C061365:flusilazole
keywords: Allium cepa; Flusilazole; Genotoxicity; Mitotic index; RAPD-PCR