title: Copaiba oil suppresses inflammatory cytokines in splenocytes of C57Bl/6 mice induced with experimental autoimmune encephalomyelitis (EAE).
abstract: Experimental autoimmune encephalomyelitis (EAE) is a murine autoimmune disease used to study multiple sclerosis. We have investigated the immunomodulatory effects of copaiba oil (100, 50 and 25 µg/mL) on NO, H2O2, TNF-α, IFN-γ and IL-17 production in cultured cells from EAE-mice. Copaiba oil (100 µg/mL) inhibited H2O2, NO, IFN-γ TNF-α and IL-17 production spontaneously or after ConA and MOG35-55 stimulation. It is suggested that copaiba oil acts on the mechanism of development of EAE by IFN-γ, IL-17 and TNF-α inhibition, modulating the immune response on both Th1 and Th17 cells.
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora, R. José Lourenço Kelmer s/n, Campus Universitário, 36036-900 Juiz de Fora, MG, Brazil.
authors.forename: Débora S
authors.initials: DS
authors.lastname: Dias
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora, R. José Lourenço Kelmer s/n, Campus Universitário, 36036-900 Juiz de Fora, MG, Brazil.
authors.forename: Lívia B A
authors.initials: LB
authors.lastname: Fontes
authors.affiliation: Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, 14040-901 Ribeirão Preto, SP, Brazil.
authors.forename: Antônio E M
authors.initials: AE
authors.lastname: Crotti
authors.affiliation: Laboratory of Experimental Imunology and Patology, CBR, Federal University of Juiz de Fora, Juiz de Fora, R. José Lourenço Kelmer s/n, Campus Universitário, 36036-900 Juiz de Fora, MG, Brazil.
authors.forename: Beatriz J V
authors.initials: BJ
authors.lastname: Aarestrup
authors.affiliation: Laboratory of Experimental Imunology and Patology, CBR, Federal University of Juiz de Fora, Juiz de Fora, R. José Lourenço Kelmer s/n, Campus Universitário, 36036-900 Juiz de Fora, MG, Brazil.
authors.forename: Fernando M
authors.initials: FM
authors.lastname: Aarestrup
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora, R. José Lourenço Kelmer s/n, Campus Universitário, 36036-900 Juiz de Fora, MG, Brazil. ademar.alves@ufjf.edu.br.
authors.forename: Ademar A
authors.initials: AA
authors.lastname: da Silva Filho
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora, R. José Lourenço Kelmer s/n, Campus Universitário, 36036-900 Juiz de Fora, MG, Brazil. joacorrea@gmail.com.
authors.forename: José O A
authors.initials: JO
authors.lastname: Corrêa
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D004353:Drug Evaluation, Preclinical; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000276:immunology; D007887:Fabaceae / Q000737:chemistry; D005260:Female; D006861:Hydrogen Peroxide / Q000378:metabolism; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000378:metabolism; D010938:Plant Oils / Q000494:pharmacology*; D013154:Spleen / Q000473:pathology*
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D010938:Plant Oils; D009569:Nitric Oxide; D006861:Hydrogen Peroxide