title: Hedgehog signaling pathway regulated the target genes for adipogenesis in silkworm Bombyx mori.
abstract: Hedgehog (Hh) signals regulate invertebrate and vertebrate development, yet the role of the pathway in adipose development remains poorly understood. In this report, we found that Hh pathway components are expressed in the fat body of silkworm larvae. Functional analysis of these components in a BmN cell line model revealed that activation of the Hh gene stimulated transcription of Hh pathway components, but inhibited the expression of the adipose marker gene AP2. Conversely, specific RNA interference-mediated knockdown of Hh resulted in increased AP2 expression. This further showed the regulation of Hh signal on the adipose marker gene. In silkworm larval models, enhanced adipocyte differentiation and an increase in adipocyte cell size were observed in silkworms that had been treated with a specific Hh signaling pathway antagonist, cyclopamine. The fat-body-specific Hh blockade tests were consistent with Hh signaling inhibiting silkworm adipogenesis. Our results indicate that the role of Hh signaling in inhibiting fat formation is conserved in vertebrates and invertebrates.
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liang
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Rui-Ting
authors.initials: RT
authors.lastname: Chen
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Deng-Pan
authors.initials: DP
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Hu-Hu
authors.initials: HH
authors.lastname: Xin
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Mei-Xian
authors.initials: MX
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yun-Gen
authors.initials: YG
authors.lastname: Miao
mesh_terms: D017667:Adipocytes / Q000502:physiology; D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D012831:Bombyx / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D005216:Fat Body / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D034622:RNA Interference; D015398:Signal Transduction / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics; D014704:Veratrum Alkaloids / Q000494:pharmacology
chemical_list: D053823:Hedgehog Proteins; D014157:Transcription Factors; D014704:Veratrum Alkaloids; C000541:cyclopamine
keywords: Bombyx mori; Hedgehog (Hh); adipocyte differentiation; adipogenesis; signaling pathway; silkworm