title: Support of hepatic regeneration by trophic factors from liver-derived mesenchymal stromal/stem cells.
abstract: Mesenchymal stromal/stem cells (MSCs) have multilineage differentiation potential and as such are known to promote regeneration in response to tissue injury. However, accumulating evidence indicates that the regenerative capacity of MSCs is not via transdifferentiation but mediated by their production of trophic and other factors that promote endogenous regeneration pathways of the tissue cells. In this chapter, we provide a detailed description on how to obtain trophic factors secreted by cultured MSCs and how they can be used in small animal models. More specific, in vivo models to study the paracrine effects of MSCs on regeneration of the liver after surgical resection and/or ischemia and reperfusion injury are described.
authors.affiliation: Department of Surgery, Laboratory of Experimental Transplantation and Intestinal Surgery (LETIS), Erasmus MC-University Medical Center, Gravendijkwal 230, 3015 CE, Rotterdam, The Netherlands.
authors.forename: Suomi M G
authors.initials: SM
authors.lastname: Fouraschen
authors.forename: Sean R R
authors.initials: SR
authors.lastname: Hall
authors.forename: Jeroen
authors.initials: J
authors.lastname: de Jonge
authors.forename: Luc J W
authors.initials: LJ
authors.lastname: van der Laan
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D064987:Cell- and Tissue-Based Therapy; D004195:Disease Models, Animal; D006498:Hepatectomy; D048550:Hepatic Insufficiency / Q000378:metabolism / Q000628:therapy; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000601:surgery; D008115:Liver Regeneration; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D015427:Reperfusion Injury