title: Salicylideneamino-2-thiophenol modulates nuclear factor-κB through redox regulation during the aging process.
abstract: OBJECTIVE
  Many intracellular components have been implicated in the regulation of redox homeostasis, but homeostasis can be unbalanced by reactive species (RS), which also probably contribute to underlying inflammatory processes. Nuclear factor-κB (NF-κB) is a well-known redox-sensitive transcription factor that controls the genes responsible for regulating inflammation.
  
  
  METHODS
  In the present study, the authors investigated the effect of short-term salicylideneamino-2-thiophenol (SAL-2) administration on the modulation of pro-inflammatory NF-κB through redox regulation in aged rats. In addition, we compared the effects of SAL-2 and caloric restriction (CR) on inflammation and redox balance. The subjects were 24-month-old (old) Fischer 344 rats administered SAL-2 (10 mg/kg/day) by dietary supplementation or placed on a 30% restricted diet for 10 days, and 6-month-old (young) rats fed ad libitum for 10 days.
  
  
  RESULTS
  We found that NF-κB activation and the expressions of its related genes (vascular cell adhesion molecule-1, intercellular adhesion molecule-1, cyclooxygenase-2 and inducible nitric oxide synthase) were suppressed by SAL-2 supplementation in old CR rats to the levels observed in young rats. In addition, our molecular studies showed that the inhibitory effect of SAL-2 on the activation of NF-κB was mediated by the ability of SAL-2 to block the nuclear translocations of cytosolic thioredoxin and redox factor-1.
  
  
  CONCLUSIONS
  These findings strongly indicate that SAL-2 stabilizes age-related redox imbalance and modulates the signal transduction pathway involved in the age-associated molecular inflammatory process.
authors.affiliation: Molecular Inflammation Research Center for Aging Intervention (MRCA), Pusan National University, Busan, Korea.
authors.forename: Bokyung
authors.initials: B
authors.lastname: Sung
authors.forename: Seongjoon
authors.initials: S
authors.lastname: Park
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Ha
authors.forename: Dae Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Chan Hum
authors.initials: CH
authors.lastname: Park
authors.forename: Kyung Jin
authors.initials: KJ
authors.lastname: Jung
authors.forename: Min Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: You Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Mi Kyung
authors.initials: MK
authors.lastname: Kim
authors.forename: Jeon-Ok
authors.initials: JO
authors.lastname: Moon
authors.forename: Takako
authors.initials: T
authors.lastname: Yokozawa
authors.forename: Nam Deuk
authors.initials: ND
authors.lastname: Kim
authors.forename: Byung Pal
authors.initials: BP
authors.lastname: Yu
authors.forename: Hae Young
authors.initials: HY
authors.lastname: Chung
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D031204:Caloric Restriction; D024202:Electrophoretic Mobility Shift Assay; D005786:Gene Expression Regulation / Q000502:physiology; D008297:Male; D016328:NF-kappa B / Q000187:drug effects*; D010084:Oxidation-Reduction; D011916:Rats, Inbred F344; D012459:Salicylates / Q000494:pharmacology*; D015398:Signal Transduction / Q000502:physiology; D013438:Sulfhydryl Compounds / Q000494:pharmacology*
chemical_list: D016328:NF-kappa B; D012459:Salicylates; D013438:Sulfhydryl Compounds; C532534:salicylideneamino-2-thiophenol
keywords: aging; inflammatory mediators; nuclear factor-κB; redox; salicylideneamino-2-thiophenol