title: Co-immobilization of Pseudomonas stutzeri YHA-13 and Alcaligenes sp. ZGED-12 with polyvinyl alcohol-alginate for removal of nitrogen and phosphorus from synthetic wastewater.
abstract: Nitrogen (N) and phosphorus (P) are the two main factors causing water eutrophication. Immobilized micro-organisms have been widely studied in N and P removal. However, the effects of various immobilizing conditions on the removal efficiency of N and P using immobilized micro-organism beads (IMOBs) remain unclear. Polyvinyl alcohol (PVA) and alginate, as the two frequently immobilizing-used matrixes, were used for co-immobilizing Pseudomonas stutzeri YHA-13 and Alcaligenes sp. ZGED-12. PVA, alginate and CaCl₂contents, immobilization time and different wet biomass ratios of P. stutzeri to Alcaligenes sp. were conducted to elucidate their roles in and influences on the removal efficiency of N and P from synthetic wastewater. The application potential of IMOBs was estimated as well. Results showed that IMOBs prepared by cross-link of 4% PVA and 2-3% alginate with 5% CaCl₂and saturated boric acid solution for 10-15 min are the best ones in removal of N and P. Though IMOBs containing P. stutzeri and/or Alcaligenes sp. were capable of removal of the two nutrients, the highest removal efficiency was observed when the wet biomass ratio of P. stutzeri to Alcaligenes sp. was adjusted to 2:2. In addition, the IMOBs were of good ability to remove chemical oxygen demand (COD), NO(3)(-), NO(2)(-), NH(4)(+)- N, total nitrogen (TN) and total phosphorus (TP) from artificial wastewater. Of which, micro-organisms immobilized in matrixes were mainly responsible for NO(3)(-) and TP removal. Therefore, P. stutzeri YHA-13 and Alcaligenes sp. ZGED-12 are reliable bioresources to remove N and P from wastewater.
authors.affiliation: a College of Life Sciences , Fujian Normal University , Fuzhou , People's Republic of China.
authors.forename: Yonghe
authors.initials: Y
authors.lastname: Han
authors.forename: Wenxian
authors.initials: W
authors.lastname: Zhang
authors.forename: Wenxian
authors.initials: W
authors.lastname: Lu
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Zhou
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zhuang
authors.forename: Min
authors.initials: M
authors.lastname: Li
mesh_terms: D000421:Alcaligenes / Q000378:metabolism*; D000464:Alginates; D002122:Calcium Chloride; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D009584:Nitrogen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D011142:Polyvinyl Alcohol; D044223:Pseudomonas stutzeri / Q000378:metabolism*; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000378:metabolism
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D014874:Water Pollutants, Chemical; D010758:Phosphorus; D020723:Glucuronic Acid; D011142:Polyvinyl Alcohol; D002122:Calcium Chloride; D009584:Nitrogen
keywords: Alcaligenes sp; Pseudomonas stutzeri; immobilization; nitrogen; phosphorus