title: Selective covalent labeling of miRNA and siRNA duplexes using HEN1 methyltransferase.
abstract: MicroRNAs regulate gene expression in numerous biological pathways and are typically methylated at their 3'-termini in plants but not in animals. Here we show that the HEN1 RNA 2'-O-methyltransferase from Arabidopsis thaliana catalyzes the transfer of extended propargylic moieties from synthetic AdoMet cofactor analogs to duplex miRNAs or siRNAs. The presented approach permits selective and efficient covalent labeling of small RNA duplexes with a variety of functional or reporter groups for their enrichment and analysis.
authors.affiliation: Institute of Biotechnology, Vilnius University , LT-02241 Vilnius, Lithuania.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Plotnikova
authors.forename: Aleksandr
authors.initials: A
authors.lastname: Osipenko
authors.forename: Viktoras
authors.initials: V
authors.lastname: Masevičius
authors.forename: Giedrius
authors.initials: G
authors.lastname: Vilkaitis
authors.forename: Saulius
authors.initials: S
authors.lastname: Klimašauskas
mesh_terms: D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D055162:Biocatalysis; D008780:Methyltransferases / Q000378:metabolism*; D035683:MicroRNAs / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D034741:RNA, Small Interfering / Q000737:chemistry / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D029681:Arabidopsis Proteins; C450825:HEN1 protein, Arabidopsis; D035683:MicroRNAs; D034741:RNA, Small Interfering; D008780:Methyltransferases; C052744:RNA 2'-O-methyltransferase