title: A test of double interspecific introgression of nucleoporin genes in Drosophila.
abstract: In interspecific hybrids between Drosophila melanogaster and Drosophila simulans, the D. simulans nucleoporin-encoding Nup96(sim) and Nup160(sim) can cause recessive lethality if the hybrid does not also inherit the D. simulans X chromosome. In addition, Nup160(sim) leads to recessive female sterility in the D. melanogaster genetic background. Here, we conducted carefully controlled crosses to better understandthe relationship between Nup96(sim) and Nup160(sim). Nup96(sim) did not lead to female sterility in the D. melanogaster genetic background, and double introgression of Nup96(sim) and Nup160(sim) did not generally lead to lethality when one was heterozygous and the other homozygous (hemizygous). It appears that introgression of additional autosomal D. simulans genes is necessary to cause lethality and that the effect of the introgression is dominant to D. melanogaster alleles. Interestingly, the genetic background affected dominance of Nup96(sim), and double introgression carrying homozygous Nup96(sim) and hemizygous Nup160(sim) resulted in lethality. Thus, Nup96(sim) and Nup160(sim) seem to be two components of the same incompatibility.
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572 sawamura@biol.tsukuba.ac.jp.
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Sawamura
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Maehara
authors.affiliation: Department of Biology, Chiba University, Chiba, Chiba 263-8522.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Keira
authors.affiliation: Department of Biology, Chiba University, Chiba, Chiba 263-8522.
authors.forename: Hiroyuki O
authors.initials: HO
authors.lastname: Ishikawa
authors.affiliation: Department of Biological Sciences, Osaka University, Toyonaka, Osaka, Japan 560-0043.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sasamura
authors.affiliation: Department of Biological Sciences, Osaka University, Toyonaka, Osaka, Japan 560-0043.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yamakawa
authors.affiliation: Department of Biological Sciences, Osaka University, Toyonaka, Osaka, Japan 560-0043.
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsuno
mesh_terms: D000818:Animals; D002678:Chimera / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D005799:Genes, Dominant; D006579:Heterozygote; D006720:Homozygote; D028861:Nuclear Pore Complex Proteins / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; D028861:Nuclear Pore Complex Proteins
keywords: Drosophila; hybrid inviability; hybrid sterility; nucleoporin; reproductive isolation; speciation