title: Intracerebral Administration of BDNF Protects Rat Brain Against Oxidative Stress Induced by Ouabain in an Animal Model of Mania.
abstract: Several studies have suggested that alterations in brain-derived neurotrophic factor (BDNF) and increased oxidative stress have a central role in bipolar disorder (BD). Intracerebroventricular (ICV) injection of ouabain (OUA) in rats alters oxidative stress parameters and decreases BDNF levels in the brain. In this context, the present study aims to investigate the effects of BDNF ICV administration on BDNF levels and oxidative stress parameters in brains of rats submitted to animal model of mania induced by OUA. Wistar rats received an ICV injection of OUA, artificial cerebrospinal fluid (ACSF), OUA plus BDNF, or ACSF plus BDNF. Locomotor activity and risk-taking behavior in the rats were measured using the open-field test. In addition, we analyzed the BDNF levels and oxidative stress parameters (TBARS, Carbonyl, CAT, SOD, GR, and GPx) in the frontal cortex and hippocampus of rats. The BDNF was unable to reverse the ouabain-induced hyperactivity and risk-taking behavior. Nevertheless, BDNF treatment increased BDNF levels, modulated the antioxidant enzymes, and protected the OUA-induced oxidative damage in the brain of rats. These results suggest that BDNF alteration observed in BD patients may be associated with oxidative damage, both seen in this disorder.
authors.affiliation: Laboratório de Neurociências, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, 88806-000, Criciúma, SC, Brazil, samirasv@yahoo.com.br.
authors.forename: Samira S
authors.initials: SS
authors.lastname: Valvassori
authors.forename: Camila O
authors.initials: CO
authors.lastname: Arent
authors.forename: Amanda V
authors.initials: AV
authors.lastname: Steckert
authors.forename: Roger B
authors.initials: RB
authors.lastname: Varela
authors.forename: Luciano K
authors.initials: LK
authors.lastname: Jornada
authors.forename: Paula T
authors.initials: PT
authors.lastname: Tonin
authors.forename: Josiane
authors.initials: J
authors.lastname: Budni
authors.forename: Edemilson
authors.initials: E
authors.lastname: Mariot
authors.forename: Flávio
authors.initials: F
authors.lastname: Kapczinski
authors.forename: João
authors.initials: J
authors.lastname: Quevedo
mesh_terms: D000818:Animals; D001714:Bipolar Disorder / Q000188:drug therapy* / Q000473:pathology* / Q000503:physiopathology; D001921:Brain / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology; D019208:Brain-Derived Neurotrophic Factor / Q000008:administration & dosage* / Q000627:therapeutic use*; D004195:Disease Models, Animal; D006624:Hippocampus / Q000201:enzymology / Q000473:pathology; D006801:Humans; D007276:Injections, Intraventricular; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D000066829:Neuroprotection; D010042:Ouabain; D018384:Oxidative Stress / Q000187:drug effects; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D017392:Thiobarbituric Acid Reactive Substances; D010042:Ouabain