title: Biocatalytic carbon capture via reversible reaction cycle catalyzed by isocitrate dehydrogenase.
abstract: The practice of carbon capture and storage (CCS) requires efficient capture and separation of carbon dioxide from its gaseous mixtures such as flue gas, followed by releasing it as a pure gas which can be subsequently compressed and injected into underground storage sites. This has been mostly achieved via reversible thermochemical reactions which are generally energy-intensive. The current work examines a biocatalytic approach for carbon capture using an NADP(H)-dependent isocitrate dehydrogenase (ICDH) which catalyzes reversibly carboxylation and decarboxylation reactions. Different from chemical carbon capture processes that rely on thermal energy to realize purification of carbon dioxide, the biocatalytic strategy utilizes pH to leverage the reaction equilibrium, thereby realizing energy-efficient carbon capture under ambient conditions. Results showed that over 25 mol of carbon dioxide could be captured and purified from its gas mixture for each gram of ICDH applied for each carboxylation/decarboxylation reaction cycle by varying pH between 6 and 9. This work demonstrates the promising potentials of pH-sensitive biocatalysis as a green-chemistry route for carbon capture.
authors.affiliation: Department of Bioproducts and Biosystems Engineering, University of Minnesota, St Paul, MN 55108, USA; Biotechnology Institute, University of Minnesota, St Paul, MN 55108, USA.
authors.forename: Shunxiang
authors.initials: S
authors.lastname: Xia
authors.affiliation: Department of Bioproducts and Biosystems Engineering, University of Minnesota, St Paul, MN 55108, USA; Biotechnology Institute, University of Minnesota, St Paul, MN 55108, USA.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Frigo-Vaz
authors.affiliation: Department of Bioproducts and Biosystems Engineering, University of Minnesota, St Paul, MN 55108, USA; Biotechnology Institute, University of Minnesota, St Paul, MN 55108, USA.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul, Korea.
authors.forename: Jungbae
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Bioproducts and Biosystems Engineering, University of Minnesota, St Paul, MN 55108, USA; Biotechnology Institute, University of Minnesota, St Paul, MN 55108, USA.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D055162:Biocatalysis; D002244:Carbon / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007521:Isocitrate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D002244:Carbon; D007521:Isocitrate Dehydrogenase
keywords: Biocatalysis; Biochemical reaction equilibrium; Carbon capture and storage; Carbon dioxide; Isocitrate dehydrogenase; Mesocellular silicon