title: Biodegradation of complex hydrocarbons in spent engine oil by novel bacterial consortium isolated from deep sea sediment.
abstract: Complex hydrocarbon and aromatic compounds degrading marine bacterial strains were isolated from deep sea sediment after enrichment on spent engine (SE) oil. Phenotypic characterization and phylogenetic analysis of 16S rRNA gene sequences showed the isolates were related to members of the Pseudoalteromonas sp., Ruegeria sp., Exiguobacterium sp. and Acinetobacter sp. Biodegradation using 1% (v/v) SE oil with individual and mixed strains showed the efficacy of SE oil utilization within a short retention time. The addition of non-ionic surfactant 0.05% (v/v) Tween 80 as emulsifying agent enhanced the solubility of hydrocarbons and renders them more accessible for biodegradation. The degradation of several compounds and the metabolites formed during the microbial oxidation process were confirmed by Fourier transform infrared spectroscopy and Gas chromatography-mass spectrometry analyses. The potential of this consortium to biodegrade SE oil with and without emulsifying agent provides possible application in bioremediation of oil contaminated marine environment.
authors.affiliation: Marine Biotechnology Division, Ocean Science and Technology for Islands Group, ESSO - National Institute of Ocean Technology, Ministry of Earth Sciences (MoES), Government of India, Pallikaranai, Chennai 600100, India.
authors.forename: A
authors.initials: A
authors.lastname: Ganesh Kumar
authors.affiliation: Marine Biotechnology Division, Ocean Science and Technology for Islands Group, ESSO - National Institute of Ocean Technology, Ministry of Earth Sciences (MoES), Government of India, Pallikaranai, Chennai 600100, India.
authors.forename: Lakshmi
authors.initials: L
authors.lastname: Vijayakumar
authors.affiliation: Marine Biotechnology Division, Ocean Science and Technology for Islands Group, ESSO - National Institute of Ocean Technology, Ministry of Earth Sciences (MoES), Government of India, Pallikaranai, Chennai 600100, India.
authors.forename: Gajendra
authors.initials: G
authors.lastname: Joshi
authors.affiliation: Marine Biotechnology Division, Ocean Science and Technology for Islands Group, ESSO - National Institute of Ocean Technology, Ministry of Earth Sciences (MoES), Government of India, Pallikaranai, Chennai 600100, India.
authors.forename: D
authors.initials: D
authors.lastname: Magesh Peter
authors.affiliation: Marine Biotechnology Division, Ocean Science and Technology for Islands Group, ESSO - National Institute of Ocean Technology, Ministry of Earth Sciences (MoES), Government of India, Pallikaranai, Chennai 600100, India.
authors.forename: G
authors.initials: G
authors.lastname: Dharani
authors.affiliation: Marine Biotechnology Division, Ocean Science and Technology for Islands Group, ESSO - National Institute of Ocean Technology, Ministry of Earth Sciences (MoES), Government of India, Pallikaranai, Chennai 600100, India. Electronic address: kiruba@niot.res.in.
authors.forename: R
authors.initials: R
authors.lastname: Kirubagaran
mesh_terms: D000150:Acinetobacter / Q000235:genetics / Q000378:metabolism; D056449:Bacillales / Q000235:genetics / Q000378:metabolism; D001419:Bacteria / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D005583:Fourier Analysis; D008401:Gas Chromatography-Mass Spectrometry; D019015:Geologic Sediments / Q000382:microbiology*; D006838:Hydrocarbons / Q000032:analysis / Q000378:metabolism*; D054327:Lubricants / Q000032:analysis*; D008969:Molecular Sequence Data; D010578:Petroleum / Q000032:analysis*; D011136:Polysorbates / Q000494:pharmacology; D031161:Pseudoalteromonas / Q000235:genetics / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012995:Solubility / Q000187:drug effects; D013055:Spectrophotometry, Infrared
chemical_list: D006838:Hydrocarbons; D054327:Lubricants; D010578:Petroleum; D011136:Polysorbates; D012336:RNA, Ribosomal, 16S
keywords: Aromatic hydrocarbons; Biodegradation; Bioremediation; Deep sea bacteria; Spent engine oil