title: WblAch, a pivotal activator of natamycin biosynthesis and morphological differentiation in Streptomyces chattanoogensis L10, is positively regulated by AdpAch.
abstract: Detailed mechanisms of WhiB-like (Wbl) proteins involved in antibiotic biosynthesis and morphological differentiation are poorly understood. Here, we characterize the role of WblAch, a Streptomyces chattanoogensis L10 protein belonging to this superfamily. Based on DNA microarray data and verified by real-time quantitative PCR (qRT-PCR), the expression of wblAch was shown to be positively regulated by AdpAch. Gel retardation assays and DNase I footprinting experiments showed that AdpAch has specific DNA-binding activity for the promoter region of wblAch. Gene disruption and genetic complementation revealed that WblAch acts in a positive manner to regulate natamycin production. When wblAch was overexpressed in the wild-type strain, the natamycin yield was increased by ∼30%. This provides a strategy to generate improved strains for natamycin production. Moreover, transcriptional analysis showed that the expression levels of whi genes (including whiA, whiB, whiH, and whiI) were severely depressed in the ΔwblAch mutant, suggesting that WblAch plays a part in morphological differentiation by influencing the expression of the whi genes.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, China Key Laboratory of Microbial Biochemistry and Metabolism Engineering of Zhejiang Province, Hangzhou, China.
authors.forename: Pin
authors.initials: P
authors.lastname: Yu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, China Key Laboratory of Microbial Biochemistry and Metabolism Engineering of Zhejiang Province, Hangzhou, China.
authors.forename: Shui-Ping
authors.initials: SP
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, China Key Laboratory of Microbial Biochemistry and Metabolism Engineering of Zhejiang Province, Hangzhou, China.
authors.forename: Qing-Ting
authors.initials: QT
authors.lastname: Bu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, China Key Laboratory of Microbial Biochemistry and Metabolism Engineering of Zhejiang Province, Hangzhou, China.
authors.forename: Zhen-Xing
authors.initials: ZX
authors.lastname: Zhou
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, China Key Laboratory of Microbial Biochemistry and Metabolism Engineering of Zhejiang Province, Hangzhou, China.
authors.forename: Zhen-Hong
authors.initials: ZH
authors.lastname: Zhu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, China.
authors.forename: Fang-Liang
authors.initials: FL
authors.lastname: Huang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, China Key Laboratory of Microbial Biochemistry and Metabolism Engineering of Zhejiang Province, Hangzhou, China lyq@zju.edu.cn.
authors.forename: Yong-Quan
authors.initials: YQ
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D010866:Natamycin / Q000096:biosynthesis*; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D015534:Trans-Activators; D010866:Natamycin