title: Preparation, characterization and in vitro cytotoxicity assay of curcumin loaded solid lipid nanoparticle in IMR32 neuroblastoma cell line.
abstract: Curcumin (diferuloylmethane) possesses low bioavailability due to its poor solubility, permeability and rapid metabolism. Solid Lipid Nanoparticle of curcumin was prepared by high-speed homogenization technique. Stearic acid was used as a lipid, tween 80 as surfactant and various co surfactants were used for the preparation of SLN. The prepared SLN was characterized using zeta sizer, TEM analysis and the average particle size was found to be in the range of 80 nm - 200nm. The entrapment efficiency of the SLN was ~58 to 85%. The characteristic FTIR peaks suggest that the stearic acid is compatible with curcumin. MTT assay was performed on the optimized formulation and the results are indicative that curcumin SLN showed better cytotoxicity in low dose while compared to plain curcumin. The developed Cu-SLN can find its better place in the anticancer therapy.
authors.affiliation: Department of Pharmaceutics, PSG College of Pharmacy, Peelamedu, Coimbatore, India.
authors.forename: Mohamed Habibur
authors.initials: MH
authors.lastname: Rahman
authors.affiliation: Department of Pharmacology, PSG College of Pharmacy, Peelamedu, Coimbatore, India.
authors.forename: Muthiah
authors.initials: M
authors.lastname: Ramanathan
authors.affiliation: Department of Pharmaceutics, PSG College of Pharmacy, Peelamedu, Coimbatore, India.
authors.forename: Veintramuthu
authors.initials: V
authors.lastname: Sankar
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D003474:Curcumin / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage; D009447:Neuroblastoma / Q000188:drug therapy* / Q000473:pathology; D010316:Particle Size; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013229:Stearic Acids / Q000008:administration & dosage
chemical_list: D000970:Antineoplastic Agents; D013229:Stearic Acids; C031183:stearic acid; D003474:Curcumin