title: Biomarker responses in sunfish species and largemouth bass from the Saluda River, South Carolina.
abstract: The upstate and Piedmont region of South Carolina is a rapidly urbanizing area as a result of a steadily growing population. This increase in population and development has the potential to negatively impact local aquatic systems like the Saluda River due to increased pollution from runoff, and effluents from industrial and wastewater treatment facilities. During the summer months of 2010, 159 fish from the Centrarchidae family (sunfish species (Lepomis) and largemouth bass - Micropterus salmoides) were collected from 13 sites along the Saluda River. A suite of biomarker assays, including ethoxyresosufin-O-deethylase, bile fluorescence, glutathione S-transferase, thiobarbituric acid reactive substances, bile estrogens, acetylcholinesterase inhibition, metallothionein and tissue metal levels were applied to investigate the impacts of diminished water quality on fish health. Results indicate that fish from the Saluda River are responding to contamination in a site specific manner, with up to four significant biomarker responses in the most impacted sites. Sampling sites in the lower portion of the Saluda watershed are less impacted by pollution than the upper and central sections. The observed biomarker responses can be explained by the proximity of urban areas, point sources and general land use, and demonstrate the applicability of biomarkers in environmental biomonitoring programs.
authors.affiliation: Graduate Program in Environmental Toxicology, Clemson University, 132 Long Hall, Clemson, SC 29634, USA.
authors.forename: Jessica
authors.initials: J
authors.lastname: Mierzejewski
authors.affiliation: Department of Biology, Furman University, 3300 Poinsett Hwy, Greenville, SC 29613, USA.
authors.forename: Dennis C
authors.initials: DC
authors.lastname: Haney
authors.affiliation: Graduate Program in Environmental Toxicology, Clemson University, 132 Long Hall, Clemson, SC 29634, USA. Electronic address: pvdhurk@clemson.edu.
authors.forename: Peter
authors.initials: P
authors.lastname: van den Hurk
mesh_terms: D000818:Animals; D001492:Bass / Q000378:metabolism*; D001646:Bile / Q000737:chemistry; D015415:Biomarkers / Q000032:analysis; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis; D005260:Female; D008297:Male; D008668:Metallothionein / Q000032:analysis; D010473:Perciformes / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis; D011960:Receptors, Estrogen / Q000378:metabolism; D045483:Rivers; D013022:South Carolina; D014876:Water Pollution
chemical_list: D015415:Biomarkers; D011084:Polycyclic Aromatic Hydrocarbons; D011960:Receptors, Estrogen; D008668:Metallothionein; D019363:Cytochrome P-450 CYP1A1
keywords: Biomarkers; Biomonitoring; Lepomis; Micropterus; Point sources; Urbanization