title: Bicaudal d family adaptor proteins control the velocity of Dynein-based movements.
abstract: Cargo transport along microtubules is driven by the collective function of microtubule plus- and minus-end-directed motors (kinesins and dyneins). How the velocity of cargo transport is driven by opposing teams of motors is still poorly understood. Here, we combined inducible recruitment of motors and adaptors to Rab6 secretory vesicles with detailed tracking of vesicle movements to investigate how changes in the transport machinery affect vesicle motility. We find that the velocities of kinesin-based vesicle movements are slower and more homogeneous than those of dynein-based movements. We also find that Bicaudal D (BICD) adaptor proteins can regulate dynein-based vesicle motility. BICD-related protein 1 (BICDR-1) accelerates minus-end-directed vesicle movements and affects Rab6 vesicle distribution. These changes are accompanied by reduced axonal outgrowth in neurons, supporting their physiological importance. Our study suggests that adaptor proteins can modulate the velocity of dynein-based motility and thereby control the distribution of transport carriers.
authors.affiliation: Department of Neuroscience, Erasmus Medical Center, Rotterdam, the Netherlands.
authors.forename: Max A
authors.initials: MA
authors.lastname: Schlager
authors.affiliation: Cell Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
authors.forename: Andrea
authors.initials: A
authors.lastname: Serra-Marques
authors.affiliation: Cell Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
authors.forename: Ilya
authors.initials: I
authors.lastname: Grigoriev
authors.affiliation: Cell Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
authors.forename: Laura F
authors.initials: LF
authors.lastname: Gumy
authors.affiliation: Cell Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
authors.forename: Marta
authors.initials: M
authors.lastname: Esteves da Silva
authors.affiliation: Cell Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
authors.forename: Phebe S
authors.initials: PS
authors.lastname: Wulf
authors.affiliation: Cell Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands. Electronic address: a.akhmanova@uu.nl.
authors.forename: Anna
authors.initials: A
authors.lastname: Akhmanova
authors.affiliation: Department of Neuroscience, Erasmus Medical Center, Rotterdam, the Netherlands; Cell Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands. Electronic address: c.hoogenraad@uu.nl.
authors.forename: Casper C
authors.initials: CC
authors.lastname: Hoogenraad
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism*; D000818:Animals; D001370:Axonal Transport; D002478:Cells, Cultured; D004398:Dyneins / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D016547:Kinesins / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D051381:Rats
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D008869:Microtubule-Associated Proteins; D004398:Dyneins; D016547:Kinesins