title: Performance evaluation of the digital cell imaging analyzer DI-60 integrated into the fully automated Sysmex XN hematology analyzer system.
abstract: BACKGROUND
  The XN-Series (Sysmex, Kobe, Japan) have been equipped with the automated digital cell imaging analyzer DI-60, which provides complete automation of the sample processing with automated complete blood counts (CBC), slide making/staining, and digital scanning with cell pre-classification. The aim of this study was to evaluate the efficacy of the XN-Series as an integrated blood cell analysis system.
  
  
  METHODS
  White blood cell (WBC) morphological analysis by the DI-60 was evaluated using 232 blood samples from patients. Routine analysis of a total of 2000 blood samples has been performed to evaluate the processing ability of the XN-Series connected to the DI-60.
  
  
  RESULTS
  The overall analysis accuracy of pre-classification of WBC by the DI-60 was 88.4%. Good correlation was observed between final results of the DI-60 analysis and manual differentiation with high sensitivity and specificity for blasts and immature granulocytes. The sample processing time of the XN-Series, from automated CBC to cell pre-classification, was 38±1 min/single run and 165±12 min/500 CBC samples run (slide preparation rate 15.6%) with no sample hold-up at the DI-60.
  
  
  CONCLUSIONS
  The automated morphological analysis capability of the DI-60 has potential usefulness in the integrated automated hematology analysis system of XN-Series.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Tabe
authors.forename: Takemasa
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Imiko
authors.initials: I
authors.lastname: Maenou
authors.forename: Rie
authors.initials: R
authors.lastname: Nakai
authors.forename: Mayumi
authors.initials: M
authors.lastname: Idei
authors.forename: Takashi
authors.initials: T
authors.lastname: Horii
authors.forename: Takashi
authors.initials: T
authors.lastname: Miida
authors.forename: Akimichi
authors.initials: A
authors.lastname: Ohsaka
mesh_terms: D001331:Automation; D006403:Hematologic Tests / Q000295:instrumentation; D006801:Humans; D007958:Leukocyte Count / Q000295:instrumentation; D007962:Leukocytes / Q000473:pathology*