title: Induction of colitis in mice (T-cell transfer model).
abstract: Animal model of intestinal inflammation is essential for the understanding of the pathomechanisms of inflammatory bowel diseases like Crohn's disease and ulcerative colitis. Typically, naïve T-cells were transferred into immunocompromised mice and develop gut inflammation because of inappropriate downregulation. Here, we describe the isolation, purification, and transfer of CD4(+)CD25(-) T-cells into recipients to obtain an experimental colitis model for the analysis of pathogenesis.
authors.affiliation: Medical Clinic I, Research-Campus, University of Erlangen-Nuremberg, Hartmannstr. 14, 91052, Erlangen, Germany, benno.weigmann@uk-erlangen.de.
authors.forename: Benno
authors.initials: B
authors.lastname: Weigmann
mesh_terms: D019264:Adoptive Transfer / Q000009:adverse effects; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000382:microbiology / Q000637:transplantation; D003092:Colitis / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D018398:Homeodomain Proteins / Q000235:genetics; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D008822:Mice, Transgenic; D013601:T-Lymphocytes / Q000378:metabolism / Q000637:transplantation*
chemical_list: D018398:Homeodomain Proteins; D053645:Interleukin-2 Receptor alpha Subunit; C064658:RAG-1 protein