title: Spinal neurons require Islet1 for subtype-specific differentiation of electrical excitability.
abstract: BACKGROUND
  In the spinal cord, stereotypic patterns of transcription factor expression uniquely identify neuronal subtypes. These transcription factors function combinatorially to regulate gene expression. Consequently, a single transcription factor may regulate divergent development programs by participation in different combinatorial codes. One such factor, the LIM-homeodomain transcription factor Islet1, is expressed in the vertebrate spinal cord. In mouse, chick and zebrafish, motor and sensory neurons require Islet1 for specification of biochemical and morphological signatures. Little is known, however, about the role that Islet1 might play for development of electrical membrane properties in vertebrates. Here we test for a role of Islet1 in differentiation of excitable membrane properties of zebrafish spinal neurons.
  
  
  RESULTS
  We focus our studies on the role of Islet1 in two populations of early born zebrafish spinal neurons: ventral caudal primary motor neurons (CaPs) and dorsal sensory Rohon-Beard cells (RBs). We take advantage of transgenic lines that express green fluorescent protein (GFP) to identify CaPs, RBs and several classes of interneurons for electrophysiological study. Upon knock-down of Islet1, cells occupying CaP-like and RB-like positions continue to express GFP. With respect to voltage-dependent currents, CaP-like and RB-like neurons have novel repertoires that distinguish them from control CaPs and RBs, and, in some respects, resemble those of neighboring interneurons. The action potentials fired by CaP-like and RB-like neurons also have significantly different properties compared to those elicited from control CaPs and RBs.
  
  
  CONCLUSIONS
  Overall, our findings suggest that, for both ventral motor and dorsal sensory neurons, Islet1 directs differentiation programs that ultimately specify electrical membrane as well as morphological properties that act together to sculpt neuron identity.
authors.affiliation: Department of Physiology, University of Colorado Anschutz Medical Campus, RC-1 North, 7403A, Mailstop 8307, 12800 E 19th Ave,, 80045 Aurora, CO, USA. rosa.moreno@ucdenver.edu.
authors.forename: Rosa L
authors.initials: RL
authors.lastname: Moreno
authors.forename: Angeles B
authors.initials: AB
authors.lastname: Ribera
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D004625:Embryo, Nonmammalian; D055785:Gene Knockdown Techniques; D007395:Interneurons / Q000378:metabolism / Q000502:physiology; D060850:LIM-Homeodomain Proteins / Q000235:genetics / Q000502:physiology*; D008564:Membrane Potentials; D009046:Motor Neurons / Q000378:metabolism / Q000502:physiology*; D011984:Sensory Receptor Cells / Q000378:metabolism / Q000502:physiology*; D013116:Spinal Cord / Q000196:embryology* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000378:metabolism / Q000502:physiology*
chemical_list: D060850:LIM-Homeodomain Proteins; D014157:Transcription Factors; D029961:Zebrafish Proteins; C063843:insulin gene enhancer binding protein Isl-1