title: Single-cell RT-PCR and immunocytochemical detection of mechanosensitive transient receptor potential channels in acutely isolated rat odontoblasts.
abstract: OBJECTIVE
  Hydrostatic force applied to tooth pulp has long been suspected to be the direct cause of dental pain. However, the molecular and cellular identity of the transducer of the mechanical force in teeth is not clear. Growing number of literatures suggested that odontoblasts, secondary to its primary role as formation of tooth structure, might function as a cellular mechanical transducer in teeth.
  
  
  METHODS
  In order to determine whether odontoblasts could play a crucial role in transduction of hydrostatic force applied to dental pulp into electrical impulses, current study investigated the expression of stretch-activated transient receptor potential (TRP) channels in acutely isolated odontoblasts from adult rats by single cell reverse transcriptase polymerase chain reaction and immunocytochemical analysis.
  
  
  RESULTS
  As the result, expression of TRPM7 (melastatin 7) was observed in majority (87%) of odontoblasts while mRNAs for TRPC1 (canonical 1), TRPC6 (canonical 6) and TRPV4 (vanilloid 4) were detected in small subpopulations of odontoblasts. TRPM3 (melastatin 3) was not detected in our experimental set-up. Immunocytochemical analysis further revealed TRPM7 expression at protein level.
  
  
  CONCLUSIONS
  Expression of the mechanosensitive TRP channels provides additional evidence that supports the sensory roles of odontoblasts. Given that TRPM7 is a mechanosensitive ion channel with a kinase activity that plays a role in Mg(2+) homeostasis, it is possible that TRPM7 expressed in odontoblasts might play a central role in mineralization during dentin formation.
authors.affiliation: Dental Research Institute and Department of Neurobiology and Physiology, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Minsoo
authors.initials: M
authors.lastname: Kwon
authors.affiliation: Dental Research Institute and Department of Neurobiology and Physiology, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Baek
authors.affiliation: Department of Physiology, Graduate School of Medicine, Gachon University, Incheon 406-799, Republic of Korea.
authors.forename: Chul-Kyu
authors.initials: CK
authors.lastname: Park
authors.affiliation: Dental Research Institute and Department of Neurobiology and Physiology, School of Dentistry, Seoul National University, Seoul, Republic of Korea; Pain Cognitive Function Research Center, Seoul National University, Seoul, Republic of Korea. Electronic address: gehoon@snu.ac.kr.
authors.forename: Gehoon
authors.initials: G
authors.lastname: Chung
authors.affiliation: Dental Research Institute and Department of Neurobiology and Physiology, School of Dentistry, Seoul National University, Seoul, Republic of Korea; Pain Cognitive Function Research Center, Seoul National University, Seoul, Republic of Korea; Department of Brain and Cognitive Sciences, College of Natural Sciences, Seoul National University, Seoul, Republic of Korea. Electronic address: odolbae@snu.ac.kr.
authors.forename: Seog Bae
authors.initials: SB
authors.lastname: Oh
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D007150:Immunohistochemistry; D009804:Odontoblasts / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050051:Transient Receptor Potential Channels / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D050051:Transient Receptor Potential Channels
keywords: Dental pain; Mineralization; Odontoblasts; Single-cell RT-PCR; TRP channels; TRPM7