title: Super-resolution imaging of plasma membrane glycans.
abstract: Much of the physiology of cells is controlled by the spatial organization of the plasma membrane and the glycosylation patterns of its components, however, studying the distribution, size, and composition of these components remains challenging. A bioorthogonal chemical reporter strategy was used for the efficient and specific labeling of membrane-associated glycoconjugates with modified monosaccharide precursors and organic fluorophores. Super-resolution fluorescence imaging was used to visualize plasma membrane glycans with single-molecule sensitivity. Our results demonstrate a homogeneous distribution of N-acetylmannosamine (ManNAc)-, N-acetylgalactosamine (GalNAc)-, and O-linked N-acetylglucosamine (O-GlcNAc)-modified plasma membrane proteins in different cell lines with densities of several million glycans on each cell surface.
authors.affiliation: Department of Biotechnology & Biophysics, Julius Maximilian University Wuerzburg, Am Hubland, 97074 Würzburg (Germany) http://www.super-resolution.de.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Letschert
authors.forename: Antonia
authors.initials: A
authors.lastname: Göhler
authors.forename: Christian
authors.initials: C
authors.lastname: Franke
authors.forename: Nadja
authors.initials: N
authors.lastname: Bertleff-Zieschang
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Memmel
authors.forename: Sören
authors.initials: S
authors.lastname: Doose
authors.forename: Jürgen
authors.initials: J
authors.lastname: Seibel
authors.forename: Markus
authors.initials: M
authors.lastname: Sauer
mesh_terms: D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D002232:Carbocyanines / Q000737:chemistry; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D057930:Click Chemistry; D061565:Cycloaddition Reaction; D006031:Glycosylation; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D018613:Microscopy, Confocal; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism
chemical_list: C569686:Alexa Fluor 647; D000480:Alkynes; D001386:Azides; D002232:Carbocyanines; D008565:Membrane Proteins; D011134:Polysaccharides
keywords: bioorthogonal chemistry; click chemistry; membrane glycans; microscopy; super-resolution imaging