title: Osteoblastic response to pectin nanocoating on titanium surfaces.
abstract: Osseointegration of titanium implants can be improved by organic and inorganic nanocoating of the surface. The aim of our study was to evaluate the effect of organic nanocoating of titanium surface with unmodified and modified pectin Rhamnogalacturonan-Is (RG-Is) isolated from potato and apple with respect to surface properties and osteogenic response in osteoblastic cells. Nanocoatings on titanium surfaces were evaluated by scanning electron microscopy, contact angle measurements, atomic force microscopy, and X-ray photoelectron spectroscopy. The effect of coated RG-Is on cell adhesion, cell viability, bone matrix formation and mineralization was tested using SaOS-2 cells. Nanocoating with pectin RG-Is affected surface properties and in consequence changed the environment for cellular response. The cells cultured on surfaces coated with RG-Is from potato with high content of linear 1.4-linked galactose produced higher level of mineralized matrix compared with control surfaces and surfaces coated with RG-I with low content of linear 1.4-linked galactose. The study showed that the pectin RG-Is nanocoating not only changed chemical and physical titanium surface properties, but also specific coating with RG-Is containing high amount of galactan increased mineralized matrix formation of osteoblastic cells in vitro.
authors.affiliation: Research Center for Ageing and Osteoporosis, Departments of Medicine and Diagnostics, Copenhagen University Hospital Glostrup, Ndr. Ringvej 57, 2600 Glostrup, Denmark; Institute of Odontology, Faculty of Health and Medical Sciences, University of Copenhagen, Nørre Allé 20, 2200 Copenhagen N, Denmark. Electronic address: kagu@sund.ku.dk.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Gurzawska
authors.affiliation: Department of Plant Environment Sciences, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark; Copenhagen Center for Glycomics, Institute for Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen N, Denmark.
authors.forename: Rikke
authors.initials: R
authors.lastname: Svava
authors.affiliation: Microtechnology and Surface Analysis, Danish Technological Institute, Gregersensvej 8, 2630 Taastrup, Denmark.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yihua
authors.affiliation: Microtechnology and Surface Analysis, Danish Technological Institute, Gregersensvej 8, 2630 Taastrup, Denmark.
authors.forename: Kenneth Brian
authors.initials: KB
authors.lastname: Haugshøj
authors.affiliation: Dansk Fundamental Metrologi A/S, Matematiktorvet 307, 2800 Lyngby, Denmark.
authors.forename: Kai
authors.initials: K
authors.lastname: Dirscherl
authors.affiliation: Copenhagen Center for Glycomics, Institute for Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen N, Denmark.
authors.forename: Steven B
authors.initials: SB
authors.lastname: Levery
authors.affiliation: Department of Plant Environment Sciences, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
authors.forename: Inge
authors.initials: I
authors.lastname: Byg
authors.affiliation: Novozymes A/S, Krogshoejvej 36, 2880 Bagsvaerd, Denmark.
authors.forename: Iben
authors.initials: I
authors.lastname: Damager
authors.affiliation: Department of Systems Biology, Technical University of Denmark, Matematiktorvet, Building 301, Kgs. Lyngby DK-2800, Denmark.
authors.forename: Martin W
authors.initials: MW
authors.lastname: Nielsen
authors.affiliation: Department of Plant Environment Sciences, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
authors.forename: Bodil
authors.initials: B
authors.lastname: Jørgensen
authors.affiliation: Research Center for Ageing and Osteoporosis, Departments of Medicine and Diagnostics, Copenhagen University Hospital Glostrup, Ndr. Ringvej 57, 2600 Glostrup, Denmark.
authors.forename: Niklas Rye
authors.initials: NR
authors.lastname: Jørgensen
authors.affiliation: Institute of Odontology, Faculty of Health and Medical Sciences, University of Copenhagen, Nørre Allé 20, 2200 Copenhagen N, Denmark.
authors.forename: Klaus
authors.initials: K
authors.lastname: Gotfredsen
mesh_terms: D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible; D006801:Humans; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D049329:Nanostructures; D010006:Osteoblasts / Q000166:cytology*; D010368:Pectins / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D014025:Titanium
chemical_list: D020099:Coated Materials, Biocompatible; D010368:Pectins; D014025:Titanium
keywords: Nanocoatings; Osseointegration; Osteoblasts; Rhamnogalacturonan-I; Surface properties; Titanium