title: Development and efficacy of real-time PCR in the diagnosis of vivax malaria using field samples in the Republic of Korea.
abstract: The development of sensitive, rapid, and accurate diagnostic methods for vivax malaria is essential for the effective control of malaria in the Republic of Korea, where vivax malaria patients usually show low parasitemia. In this study, a TaqMan-based real-time polymerase chain reaction (PCR) method was established and compared with other PCR-based assays, including nested PCR, loop-mediated isothermal amplification, and multiplex PCR, using samples from febrile patients with suspected vivax malaria. The established real-time PCR had a high sensitivity (99.6%) and specificity (100%). Therefore, this sensitive, specific, rapid, and quantitative real-time PCR method could be used for the routine diagnosis of vivax malaria in the laboratory of the Korea National Institute of Health.
authors.affiliation: Division of Malaria and Parasitic Diseases, National Institute of Health, Korea CDC, Osong Saeng-myeong 2 ro, Osong Health Technology Administration Complex 187, Osong, Republic of Korea.
authors.forename: Jung-Yeon
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Division of Malaria and Parasitic Diseases, National Institute of Health, Korea CDC, Osong Saeng-myeong 2 ro, Osong Health Technology Administration Complex 187, Osong, Republic of Korea; Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine, Daegu, Republic of Korea.
authors.forename: Youn-Kyoung
authors.initials: YK
authors.lastname: Goo
authors.affiliation: Division of Malaria and Parasitic Diseases, National Institute of Health, Korea CDC, Osong Saeng-myeong 2 ro, Osong Health Technology Administration Complex 187, Osong, Republic of Korea.
authors.forename: So-Young
authors.initials: SY
authors.lastname: Ji
authors.affiliation: Division of Malaria and Parasitic Diseases, National Institute of Health, Korea CDC, Osong Saeng-myeong 2 ro, Osong Health Technology Administration Complex 187, Osong, Republic of Korea.
authors.forename: Hyun-Il
authors.initials: HI
authors.lastname: Shin
authors.affiliation: Department of Parasitology, Kangwon National University College of Medicine, Chuncheon, Republic of Korea.
authors.forename: Eun-Taek
authors.initials: ET
authors.lastname: Han
authors.affiliation: Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine, Daegu, Republic of Korea.
authors.forename: Yeonchul
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine, Daegu, Republic of Korea.
authors.forename: Dong-Il
authors.initials: DI
authors.lastname: Chung
authors.affiliation: Division of Malaria and Parasitic Diseases, National Institute of Health, Korea CDC, Osong Saeng-myeong 2 ro, Osong Health Technology Administration Complex 187, Osong, Republic of Korea.
authors.forename: Shin-Hyung
authors.initials: SH
authors.lastname: Cho
authors.affiliation: Division of Malaria and Parasitic Diseases, National Institute of Health, Korea CDC, Osong Saeng-myeong 2 ro, Osong Health Technology Administration Complex 187, Osong, Republic of Korea.
authors.forename: Won-Ja
authors.initials: WJ
authors.lastname: Lee
mesh_terms: D001769:Blood / Q000469:parasitology; D006801:Humans; D057230:Limit of Detection; D016780:Malaria, Vivax / Q000175:diagnosis* / Q000235:genetics / Q000469:parasitology; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D010966:Plasmodium vivax / Q000235:genetics* / Q000472:pathogenicity; D016133:Polymerase Chain Reaction / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D056910:Republic of Korea; D012680:Sensitivity and Specificity