title: Adipogenic fate commitment of muscle-derived progenitor cells: isolation, culture, and differentiation.
abstract: Skeletal muscle harbors several types of cells, among which a population of progenitors committed to the adipogenic lineage has only recently been identified. Potential sources of white and brown adipocytes, the latter representing a potential target to treat obesity, are of considerable interest to the field. Fluorescence-activated cell sorting (FACS) provides an elegant strategy for prospective isolation of closely defined cell populations. Here we describe a flow cytometric method to isolate muscle-resident adipogenic progenitor cells with a default potential to undergo white adipogenesis. We further describe an approach to induce commitment to a lineage of brown-like adipocytes upon exposure to bone morphogenetic protein 7 (BMP7).
authors.affiliation: Research Group Adipocyte Development, German Institute of Human Nutrition, Arthur-Scheunert-Allee 114-116, Nuthetal, 14558, Germany.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Lau
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Tseng
authors.forename: Tim J
authors.initials: TJ
authors.lastname: Schulz
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis; D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D064987:Cell- and Tissue-Based Therapy; D051379:Mice; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*