title: Apoptotic effects of psiRNA-STAT3 on 4T1 breast cancer cells in vitro.
abstract: BACKGROUND
  The aim of this study was to investigate the effect of a Lipofectamine2000 (Life2000) Transfection Reagent transfected psiRNA-STAT3 plasmid on 4T1 breast cancer cells.
  
  
  METHODS
  MTT was used to detect the cell proliferation of breast cancer 4T1 cells at different periods (0h, 6h, 8h, 10h); the cell cycle was assessed by flow cytometry; variation of apoptosis and mitochondrial membrane potential was observed under a fluorescence microscope; immunohistochemical staining was used to determine the expression of caspase-3 and cyclin-D1 protein.
  
  
  RESULTS
  An obvious effect of inhibition to 4T1 cancer cells could be observed at 8h after the psiRNA-STAT3 was transfected. Typical alterations of apoptotic morphological features were visible in the psiRNA-STAT3 treatment group. Mitochondrial membrane potential decreased significantly, the number of cells was increased in G0/G1 phase, and the number of cells was decreased in S phase, and the data were statistically significant (p<0.05), compared with the Scramble and Mock groups. Expression of caspase-3 protein was increased significantly, while that of cyclin D1 was significantly decreased.
  
  
  CONCLUSIONS
  Life2000 transfected psiRNA-STAT3 plasmid can inhibit 4T1 tumor cell proliferation and promote apoptosis of 4T1 tumor cells, which process depends on the regulation of expression of cyclin D1 and caspase-3 protein.
authors.affiliation: School of Pharmacy, 2Department of Breast Surgery , The Second Clinical Hospital, 3Department of Plastic Surgery, the China- Japan Union Hospital, 4Department of Biology and Medical Engineering, Institute of Regenerative Medicine, Jilin University, Changchun, China E-mail : gbf_cc@sohu.com, zhouqw@jlu.edu.cn.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhou
authors.forename: Lin
authors.initials: L
authors.lastname: Tian
authors.forename: Ying-Chao
authors.initials: YC
authors.lastname: Zhang
authors.forename: Bao-Feng
authors.initials: BF
authors.lastname: Guo
authors.forename: Qing-Wei
authors.initials: QW
authors.lastname: Zhou
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics*; D053148:Caspase 3 / Q000096:biosynthesis; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D019938:Cyclin D1 / Q000096:biosynthesis; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints / Q000235:genetics; D008055:Lipids / Q000008:administration & dosage; D053078:Membrane Potential, Mitochondrial / Q000235:genetics*; D051379:Mice; D034741:RNA, Small Interfering / Q000235:genetics; D059807:S Phase Cell Cycle Checkpoints / Q000235:genetics; D050796:STAT3 Transcription Factor / Q000008:administration & dosage / Q000235:genetics*; D014162:Transfection / Q000379:methods
chemical_list: D008055:Lipids; C086724:Lipofectamine; D034741:RNA, Small Interfering; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D019938:Cyclin D1; D053148:Caspase 3