title: Changes of cytoskeleton and cell cycle in Lovo cells via deletion of Rac1.
abstract: BACKGROUND
  Rac1, the better characterized Rac subfamily member, can regulate a large variety of different functions, including the organization of the actin cytoskeleton, cell migration, cell cycle progression, and cell survival through engagement of specific effectors. However, very little is currently known about the expression of Rac1 in colorectal cancer cells and the roles of Rac1 in the cell cycle progression and cell survival of human colorectal cancer cells.
  
  
  OBJECTIVE
  To assess the change of cytoskeleton and cell cycle in Lovo (human colorectal cancer) cell via deletion of Rac1 with RNA interference.
  
  
  METHODS
  Rac1 protein of all selected human colorectal cancer cells and in human colorectal tissue was detected by Western blotting, Rac1-shRNA was used to silence the Rac1 to reduce its expression specifically in Lovo cells.
  
  
  RESULTS
  Rac1 protein was overexpressed in human colorectal cancer cells and in human colorectal tissue, RNA interference-mediated deletion of Rac1 strongly prolonged cell cycle progression and enhanced cell apoptosis of Lovo cells in vitro.
  
  
  CONCLUSIONS
  depletion of Rac1 by the use of RNAi can arrest Lovo Cells in G<formula>_{0}</formula>/G<formula>_{1}</formula> and induce LoVo cells apoptosis.
authors.affiliation: Department of Medical Ultrasonics, The Second Hospital of Lanzhou University, Lanzhou, Gansu, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Nie
authors.affiliation: Department of Oncology, The Third people's Hospital of Jinan, Jinan, Shandong, China.
authors.forename: Shi-Yi
authors.initials: SY
authors.lastname: Zhao
authors.affiliation: Department of Oncology, The Second Hospital of Lanzhou University, Lanzhou, Gansu, China.
authors.forename: Fei-Xue
authors.initials: FX
authors.lastname: Song
authors.affiliation: Department of Oncology, The Second Hospital of Lanzhou University, Lanzhou, Gansu, China.
authors.forename: Pei-Wu
authors.initials: PW
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology; D003599:Cytoskeleton / Q000473:pathology*; D016193:G1 Phase / Q000235:genetics*; D019073:HT29 Cells; D006801:Humans; D034622:RNA Interference / Q000502:physiology; D016192:Resting Phase, Cell Cycle / Q000235:genetics*; D017384:Sequence Deletion / Q000235:genetics*; D020830:rac1 GTP-Binding Protein / Q000235:genetics*
chemical_list: C107953:RAC1 protein, human; D020830:rac1 GTP-Binding Protein
keywords: Cytoskeleton; RNA interference; Rac1; cell apoptosis; cell cycle