title: Protocol for cutaneous wound healing assay in a murine model.
abstract: Cutaneous wound healing assay is important to address many key questions including (1) migration ability of different cells; (2) communication between the different cell types such as keratinocytes, fibroblasts, and immune cells; (3) understanding the cell-autonomous and non-cell-autonomous function(s) of the different cells; and (4) gene regulation in healing processes. Wound healing studies can be used to test new treatment modalities, function of new drugs/compounds, and stem cell-based therapies on the different stages of healing and for accelerating wound healing in patients with compromised healing. In this chapter, we have described a simple step-by-step protocol to generate full-thickness cutaneous wounds in the dorsal skin of mice, followed by collecting the post-wounding biopsied materials on specific days for histological and immunohistochemical analyses and for RNA and protein extractions.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, 1601 SW Jefferson str, Corvallis, OR, 97331, USA, indrag@onid.orst.edu.
authors.forename: Gitali
authors.initials: G
authors.lastname: Ganguli-Indra
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D051379:Mice; D012867:Skin / Q000473:pathology*; D014945:Wound Healing; D014947:Wounds and Injuries / Q000473:pathology*