title: [Isolation and identification of nine iridoid glycosides from Lamiophlomis rotata by HPLC combining PDA and MS].
abstract: OBJECTIVE
  To establish a rapid chromatographic method to separate the iridoid glycosides from Lamioplomis rotata, and to identify the target compounds with PDA and MS.
  
  
  METHODS
  Methanol-water gradient elution was used to separate and analyze the target compounds. The fluid fractions were gathered according to the chromatogram and dried with the nitrogen airflow. The mass fractions of the target compounds were determined with RP-HPLC and the structures were identified with PDA and MS.
  
  
  RESULTS
  The purity of some compounds exceeded 90% and these 9 compounds were identified as iridoid glycosides, which were Phlorigidoside C (1), Schismoside (2), Sesamoside (3), Shanzhiside methylester (4), 6-O-Acetyl shanzhiside methylester (5), Phloyoside II (6), Penstemoside (7), Loganin (8) and 8-O-Acetyl shanzhiside methylester (9).
  
  
  CONCLUSIONS
  The method is simple and practicable with high efficiency. It can be used to qualitative and quantitative analysis of the 9 iridoid glycosides in Lamiphlomis rotata and its preparations.
authors.forename: Rui
authors.initials: R
authors.lastname: Tao
authors.forename: Mao-Xing
authors.initials: MX
authors.lastname: Li
authors.forename: Quan-Long
authors.initials: QL
authors.lastname: Zhang
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Qiu
authors.forename: Juan-Hong
authors.initials: JH
authors.lastname: Zhang
authors.forename: Zheng-Ping
authors.initials: ZP
authors.lastname: Jia
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000431:Ethanol / Q000737:chemistry; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification; D057889:Iridoid Glycosides / Q000737:chemistry* / Q000302:isolation & purification; D039823:Iridoids / Q000737:chemistry / Q000302:isolation & purification; D019686:Lamiaceae / Q000737:chemistry*; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011714:Pyrans / Q000737:chemistry / Q000302:isolation & purification; D012117:Resins, Synthetic / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D005960:Glucosides; D057889:Iridoid Glycosides; D039823:Iridoids; D011714:Pyrans; D012117:Resins, Synthetic; D013729:Terpenes; C407310:phloyoside II; D000431:Ethanol; C099473:8-O-acetyl shanzhiside methyl ester; C059516:loganin