title: Cytoprotective effect of kaempferol on paraquat-exposed BEAS-2B cells via modulating expression of MUC5AC.
abstract: Mucins are highly glycosylated secretary proteins produced by most epithelial cells. Hypersecretion of mucins is one of the prominent symptoms of several airway diseases, including asthma, cystic fibrosis, nasal allergy, rhinitis, and sinusitis. Paraquat (PQ), a common herbicide, has been associated with pulmonary damage and is a potent reactive oxygen species (ROS) producer. However, until now the role of PQ on mucin overproduction has not been studied. The aim of this study is to explore how kaempferol (KM), a widely used dietary flavonoid, affects the protection of human PQ-exposed bronchial epithelium BEAS-2B cells by suppressing Mucin gene expression via nuclear factor-kappa B (NF-κB). We observed that PQ generates intracellular ROS, and also induces lipid peroxidation in BEAS-2B cells. Additionally, we found that PQ effectively induces the expression of the MUC5AC gene; however, co-treatment of PQ with KM drastically reduces its expression. Furthermore, we observed that PQ activates NF-κB, while co-treatment with KM occludes its nuclear translocation, and additionally KM repressed the PQ phosphorylation of c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) in BEAS-2B cells. Based on our data, we believe that KM can suppress the over-expression of the MUC5AC gene. This would contribute to the protection of PQ cytotoxicity to exposed BEAS-2B cells, and allow further study toward a better understanding of ROS-associated diseases.
authors.affiliation: Department of Microbiology, College of Medicine, Soonchunhyang University.
authors.forename: Biswajit
authors.initials: B
authors.lastname: Podder
authors.forename: Kyoung Seob
authors.initials: KS
authors.lastname: Song
authors.forename: Ho-Yeon
authors.initials: HY
authors.lastname: Song
authors.forename: Yong-Sik
authors.initials: YS
authors.lastname: Kim
mesh_terms: D002460:Cell Line; D019610:Cytoprotection / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006540:Herbicides / Q000633:toxicity*; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D044949:Kaempferols / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D055271:Mucin 5AC / Q000235:genetics*; D016328:NF-kappa B / Q000378:metabolism; D010269:Paraquat / Q000633:toxicity*; D020011:Protective Agents / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D006540:Herbicides; D044949:Kaempferols; C524678:MUC5AC protein, human; D055271:Mucin 5AC; D016328:NF-kappa B; D020011:Protective Agents; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; C006552:kaempferol; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D010269:Paraquat