title: Pioglitazone inhibits angiotensin II-induced atrial fibroblasts proliferation via NF-κB/TGF-β1/TRIF/TRAF6 pathway.
abstract: The exact mechanisms underlying inhibitory effects of pioglitazone (Pio) on Angiotensin II (AngII)-induced atrial fibrosis are complex and remain largely unknown. In the present study, we examined the effect of Pio on AngII-induced mice atrial fibrosis in vivo and atrial fibroblasts proliferation in vitro. In vivo study showed that AngII infusion induced atrial fibrosis and increased expressions of Toll/IL-1 receptor domain-containing adaptor inducing IFN-β (TRIF) and tumor necrosis factor receptor associated factor 6 (TRAF6) in mice models. However, those effects could be attenuated by Pio (P<0.01). As for in vitro experiment, Pio suppressed AngII-induced atrial fibroblasts proliferation via nuclear factor-κB/transforming growth factor-β1/TRIF/TRAF6 signaling pathway in primary cultured mice atrial fibroblasts (P<0.01). In conclusion, suppression of Pio on AngII-induced atrial fibrosis might be related to its inhibitory effects on above signaling pathway.
authors.affiliation: Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiaotong University, 241 west Huaihai Road, Shanghai 200030, People׳s Republic of China.
authors.forename: Xiao-qing
authors.initials: XQ
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiaotong University, 241 west Huaihai Road, Shanghai 200030, People׳s Republic of China. Electronic address: xkliuxu@126.com.
authors.forename: Xu
authors.initials: X
authors.lastname: Liu
authors.affiliation: National Key Laboratory of Medical Immunology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, People׳s Republic of China. Electronic address: wqxejd@126.com.
authors.forename: Quan-xing
authors.initials: QX
authors.lastname: Wang
authors.affiliation: National Key Laboratory of Medical Immunology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, People׳s Republic of China.
authors.forename: Ming-jian
authors.initials: MJ
authors.lastname: Zhang
authors.affiliation: National Key Laboratory of Medical Immunology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, People׳s Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Guo
authors.affiliation: National Key Laboratory of Medical Immunology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, People׳s Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiaotong University, 241 west Huaihai Road, Shanghai 200030, People׳s Republic of China.
authors.forename: Wei-feng
authors.initials: WF
authors.lastname: Jiang
authors.affiliation: Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiaotong University, 241 west Huaihai Road, Shanghai 200030, People׳s Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D049109:Cell Proliferation; D005355:Fibrosis / Q000378:metabolism; D006325:Heart Atria / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D058628:Myofibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D016328:NF-kappa B / Q000378:metabolism; D000077205:Pioglitazone; D015398:Signal Transduction; D048029:TNF Receptor-Associated Factor 6 / Q000378:metabolism; D045162:Thiazolidinediones / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D000804:Angiotensin II; D016328:NF-kappa B; D000077205:Pioglitazone; D048029:TNF Receptor-Associated Factor 6; D045162:Thiazolidinediones; D053773:Transforming Growth Factor beta1; C476286:Ticam1 protein, mouse
keywords: Angiotensin II; Atrial fibrosis; Pioglitazone; Signaling pathway