title: TNFR1 absence protects against memory deficit induced by sepsis possibly through over-expression of hippocampal BDNF.
abstract: The involvement of TNF-α type 1 receptor (TNFR1) in memory deficits induced by sepsis was explored by using TNFR1 knockout (KO) mice. We reported that wild type (WT) mice presented memory deficits in the novel object recognition test 10 days after sepsis induced by cecum ligation and perforation (CLP). These deficits were not observed in TNFR1 KO mice. The involvement of serum and brain cytokines TNF-α, IL-1β, IL-6, IFN-γ and IL-10 was then investigated. TNFR1 KO mice had higher serum levels of TNF-α and IL-1β, and brain levels of TNF-α than WT mice. After CLP, the brain levels of TNF-α, IL-1β, IL-6 and IFN-γ increased in both WT and KO mice. Our next step was to determine the expression of inflammatory cytokines, BDNF and TrKb in the hippocampus. The absence of TNFR1 in mice subjected to polymicrobial sepsis resulted in higher BDNF expression in the hippocampus. In conclusion, after CLP, memory is preserved in the absence of TNFR1. This finding was associated with increased BDNF expression in the hippocampus.
authors.affiliation: Interdisciplinary Laboratory of Medical Investigation, School of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil, allancalsavara@gmail.com.
authors.forename: Allan C
authors.initials: AC
authors.lastname: Calsavara
authors.forename: Frederico M
authors.initials: FM
authors.lastname: Soriani
authors.forename: Leda Q
authors.initials: LQ
authors.lastname: Vieira
authors.forename: Priscila A
authors.initials: PA
authors.lastname: Costa
authors.forename: Milene A
authors.initials: MA
authors.lastname: Rachid
authors.forename: Antônio L
authors.initials: AL
authors.lastname: Teixeira
authors.forename: Antônio L
authors.initials: AL
authors.lastname: Teixiera
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis*; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D008569:Memory Disorders / Q000378:metabolism* / Q000517:prevention & control*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000172:deficiency*; D018805:Sepsis / Q000150:complications / Q000378:metabolism*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D047888:Receptors, Tumor Necrosis Factor, Type I; C506236:Tnfrsf1a protein, mouse