title: Accumulation of CD208⁺ mature dendritic cells does not correlate with survival time in oral squamous cell carcinoma patients.
abstract: OBJECTIVE
  To investigate the distribution and clinical outcomes of tumor-infiltrating CD208(+) mature dendritic cells (mDCs) in patients with oral squamous cell carcinoma (OSCC).
  
  
  METHODS
  Using immunohistochemical analysis, the distribution of CD208(+) mDCs in adjacent non-neoplastic tissues (NTs) and tumor tissue, including the tumor stroma (TS) and tumor nest (TN), of 79 patients with OSCC was evaluated. The analysis was quantitative, and the number of positive cells was counted in 5 microscopic high-power fields (×400). At the gene expression level, CD208 expression also was assessed by quantitative polymerase chain reaction. Kaplan Meier analyses were used to analyze the prognostic value of tumor-infiltrating CD208(+) mDCs.
  
  
  RESULTS
  The number of tumor-infiltrating CD208(+) mDCs was larger in the TS and TN than in the NTs (P < .0001) and the number of CD208(+) mDCs in the TS was larger in patients with OSCC and lymph node positivity (P < .05). At the gene expression level, CD208 also was upregulated in patients with lymph node positivity (P < .05). The number of infiltrating CD208(+) mDCs was not associated with a patient's survival time.
  
  
  CONCLUSIONS
  The number of tumor-infiltrating CD208(+) mDCs in the TS was larger in patients with OSCC and lymph node positivity, but the accumulation of CD208(+) mDCs did not correlate with survival time in patients with OSCC.
authors.affiliation: Professor, Central Laboratory of Stomatology, Hospital of Stomatology and The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, Medical School, Nanjing University, Nanjing, China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Ni
authors.affiliation: Medical Doctor, Professor, Department of Oral and Maxillofacial Surgery, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing Stomatological Hospital, Nanjing, China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Huang
authors.affiliation: Student, Central Laboratory of Stomatology, Hospital of Stomatology and The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, Medical School, Nanjing University, Nanjing, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Ding
authors.affiliation: Medical Doctor, Professor, Department of Oral and Maxillofacial Surgery, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing Stomatological Hospital, Nanjing, China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Wang
authors.affiliation: Professor, Department of Oral and Maxillofacial Surgery, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing Stomatological Hospital, Nanjing, China. Electronic address: qinganghu@hotmail.com.
authors.forename: Qin-Gang
authors.initials: QG
authors.lastname: Hu
authors.affiliation: Professor, Central Laboratory of Stomatology, Hospital of Stomatology and The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, Medical School, Nanjing University, Nanjing, China.
authors.forename: Ya-Yi
authors.initials: YY
authors.lastname: Hou
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000473:pathology*; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006801:Humans; D051907:Lysosomal Membrane Proteins / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000276:immunology / Q000473:pathology*; D009363:Neoplasm Proteins / Q000276:immunology*; D016133:Polymerase Chain Reaction; D016019:Survival Analysis; D059666:Lysosomal-Associated Membrane Protein 3
chemical_list: D051907:Lysosomal Membrane Proteins; D009363:Neoplasm Proteins; C114233:LAMP3 protein, human; D059666:Lysosomal-Associated Membrane Protein 3