title: Wickerhamiella siamensis f.a., sp. nov., an endophytic and epiphytic yeast species isolated from sugar cane leaf.
abstract: Six strains representing a novel yeast species were isolated from tissue (DMKU-SE106(T), DMKU-SE110, DMKU-SE112 and DMKU-SE132) and the external surface (DMKU-SP335 and DMKU-SP406) of sugar cane leaves collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and sequence analysis of the D1/D2 region of the LSU rRNA gene and the internal transcribed spacer (ITS) region, the six strains were found to represent a single novel species of the genus Wickerhamiella although the formation of ascospores was not observed. The sequences of the D1/D2 region of the LSU rRNA gene and ITS region of the six strains differed from each other by 0-2 and 2-3 nt substitutions, respectively. The novel species was related most closely to Candida infanticola but with 4.5-4.6% nucleotide substitutions in the D1/D2 region of the LSU rRNA gene and 6.6-7.1% nucleotide substitutions in the ITS region. The name Wickerhamiella siamensis f.a., sp. nov. is proposed. The type strain is DMKU-SE106(T) ( =BCC 61185(T) =NBRC 109697(T) =CBS 13331(T)).
authors.affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: Pannida
authors.initials: P
authors.lastname: Khunnamwong
authors.affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: Janjira
authors.initials: J
authors.lastname: Surussawadee
authors.affiliation: Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathumthani, Thailand.
authors.forename: Sasitorn
authors.initials: S
authors.lastname: Jindamorakot
authors.affiliation: Center for Advanced Studied in Tropical Natural Resources, National Research University Kasetsart University, Thailand.|Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: Savitree
authors.initials: S
authors.lastname: Limtong
mesh_terms: D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D060026:Endophytes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology*; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D031786:Saccharum / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013785:Thailand
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer