title: Platelet concentration in platelet-rich plasma affects tenocyte behavior in vitro.
abstract: Since tendon injuries and tendinopathy are a growing problem, sometimes requiring surgery, new strategies that improve conservative therapies are needed. Platelet-rich plasma (PRP) seems to be a good candidate by virtue of its high content of growth factors, most of which are involved in tendon healing. This study aimed to evaluate if different concentrations of platelets in PRP have different effects on the biological features of normal human tenocytes that are usually required during tendon healing. The different platelet concentrations tested (up to 5 × 10(6) plt/µL) stimulated differently tenocytes behavior; intermediate concentrations (0.5 × 10(6), 1 × 10(6) plt/µL) strongly induced all tested processes (proliferation, migration, collagen, and MMPs production) if compared to untreated cells; on the contrary, the highest concentration had inhibitory effects on proliferation and strongly reduced migration abilities and overall collagen production but, at the same time, induced increasing MMP production, which could be counterproductive because excessive proteolysis could impair tendon mechanical stability. Thus, these in vitro data strongly suggest the need for a compromise between extremely high and low platelet concentrations to obtain an optimal global effect when inducing in vivo tendon healing.
authors.affiliation: Department of Life, Health and Environmental Sciences, University of L'Aquila, Italy.
authors.forename: Ilaria
authors.identifier: 0000-0002-7982-471X
authors.initials: I
authors.lastname: Giusti
authors.affiliation: Department of Life, Health and Environmental Sciences, University of L'Aquila, Italy.
authors.forename: Sandra
authors.identifier: 0000-0003-2820-2459
authors.initials: S
authors.lastname: D'Ascenzo
authors.affiliation: Postgraduate School in Orthopedics and Traumatology, University of L'Aquila, Italy.
authors.forename: Annalisa
authors.identifier: 0000-0002-8787-4426
authors.initials: A
authors.lastname: Mancò
authors.affiliation: Department of Life, Health and Environmental Sciences, University of L'Aquila, Italy.
authors.forename: Gabriella
authors.identifier: 0000-0002-9903-3528
authors.initials: G
authors.lastname: Di Stefano
authors.affiliation: Department of Life, Health and Environmental Sciences, University of L'Aquila, Italy.
authors.forename: Marianna
authors.identifier: 0000-0002-8418-135X
authors.initials: M
authors.lastname: Di Francesco
authors.affiliation: Immunotransfusion Medicine Unit, "San Salvatore" Hospital, L'Aquila, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Rughetti
authors.affiliation: Pathological Anatomy Unit, "San Salvatore" Hospital, L'Aquila, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Dal Mas
authors.affiliation: Department of Life, Health and Environmental Sciences, University of L'Aquila, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Properzi
authors.affiliation: Department of Life, Health and Environmental Sciences, University of L'Aquila, Italy ; Postgraduate School in Orthopedics and Traumatology, University of L'Aquila, Italy.
authors.forename: Vittorio
authors.initials: V
authors.lastname: Calvisi
authors.affiliation: Department of Life, Health and Environmental Sciences, University of L'Aquila, Italy.
authors.forename: Vincenza
authors.identifier: 0000-0003-0424-6676
authors.initials: V
authors.lastname: Dolo
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000378:metabolism* / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D003094:Collagen / Q000378:metabolism; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism; D008875:Middle Aged; D053657:Platelet-Rich Plasma / Q000378:metabolism* / Q000502:physiology*; D013710:Tendons / Q000378:metabolism* / Q000502:physiology*; D014945:Wound Healing / Q000502:physiology
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D003094:Collagen; D020782:Matrix Metalloproteinases