title: Mechanism of induction of fibroblast to corneal endothelial cell.
abstract: OBJECTIVE
  To explore mechanism of nduction of fibroblast to corneal endothelial cell.
  
  
  METHODS
  Rabbit conjunctiva fibroblasts were used as feeder cells, rabbit oral mucosa epithelial cells were used as seed cells, and human denuded amniotic membrane was used as carrier to establish tissue engineering corneal endothelium. The transformation effect was observed.
  
  
  RESULTS
  As concentration of mitomycin C increased, cell survival rate gradually decreased, cell proliferation was obviously inhibited when concentration≥25 μg/mL; 5 days after being treated by 5 μg/mL mitomycin C, cell body was enlarged and extended without cell fusion, however after being treated by 0.5 μg/mL mitomycin C, cell body was significantly proliferated and gradually fused; after 3 weeks of culture, stratified epithelium appeared on rabbit oral mucosa epithelial cells, differentiation layers were 4-5 and were well differentiated, the morphology was similar to corneal endothelial cells; Under electron microscope, surface layer of cells were polygonal, tightly connected to another with microvilli on the border, there was hemidesmosome between basal cells and human denuded amniotic membrane.
  
  
  CONCLUSIONS
  Fibroblast cells have the potential of multi-directional differentiation, effective induction can promote emergence of intercellular desmosomes between seed cells and emergence of epithelial surface microvilli, and differentiate to the corneal endothelial cell. However, clinical application still needs more research and safety evaluation.
authors.affiliation: Department of Ophthalmology, Renji Hospital Affiliated to Medical College of Shanghai Jiaotong University, Shanghai 200127, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Ophthalmology, Renji Hospital Affiliated to Medical College of Shanghai Jiaotong University, Shanghai 200127, China.
authors.forename: Wei-Cai
authors.initials: WC
authors.lastname: Fu
authors.affiliation: Department of Ophthalmology, Renji Hospital Affiliated to Medical College of Shanghai Jiaotong University, Shanghai 200127, China. Electronic address: 28906390@qq.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000650:Amnion / Q000166:cytology; D000818:Animals; D066146:Cell Body / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004728:Endothelium, Corneal / Q000166:cytology*; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D016685:Mitomycin / Q000494:pharmacology*; D009061:Mouth Mucosa / Q000166:cytology; D011817:Rabbits
chemical_list: D016685:Mitomycin
keywords: Corneal endothelial cell; Fibroblast; Induction; Transformation