title: Lophirones B and C extenuate AFB1-mediated oxidative onslaught on cellular proteins, lipids, and DNA through Nrf-2 expression.
abstract: The capability of lophirones B and C to extenuate aflatoxin B1 (AFB1)-mediated onslaught on cellular proteins, lipids, and DNA was investigated for 6 weeks. Lophirones B and C significantly (P < 0.05) increase the expression and specific activity of cytoprotective enzymes (glutathione-S-trans-ferase, nioctinamide adenine dicludeotide:quinone oxidoreductase-1, epoxide hydrolase, and uridyl glucuronosyl transferase). There was significant (P < 0.05) reduction in the level of antioxidant system in AFB1-induced hepatocarcinogenesis. Furthermore, lophirones B and C significantly (P < 0.05) attenuated AFB1-mediated decrease in the specific activities of antioxidant enzymes. Oxidative stress biomarkers, malondialdehyde, lipid hydroperoxides, conjugated dienes, protein carbonyl, and fragmented DNA were significantly (P < 0.05) elevated in AFB1-treated rats. Although lophirones B and C did not significantly (P < 0.05) alter these biomarkers, an AFB1-mediated increase in these biomarkers was significantly attenuated. Results obtained showed that lophirones B and C extenuate AFB1-mediated onslaught on cellular proteins, lipids, and DNA by enhancing nuclear erythroid-related factor-2 expression.
authors.affiliation: Antioxidants, Free Radicals, Functional Foods and Toxicology Research Laboratory, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria. ajiboyeyong@yahoo.com.
authors.forename: Taofeek O
authors.initials: TO
authors.lastname: Ajiboye
authors.forename: Musa T
authors.initials: MT
authors.lastname: Yakubu
authors.forename: Adenike T
authors.initials: AT
authors.lastname: Oladiji
mesh_terms: D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D044946:Biflavonoids / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects*; D008113:Liver Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D008297:Male; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D010088:Oxidoreductases / Q000096:biosynthesis*; D011042:Poisons / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D044946:Biflavonoids; D004273:DNA, Neoplasm; D051267:NF-E2-Related Factor 2; D009363:Neoplasm Proteins; C495637:Nfe2l2 protein, rat; D011042:Poisons; D016604:Aflatoxin B1; D010088:Oxidoreductases
keywords: Aflatoxin B1; Lophirones B and C; Nrf-2; Oxidative Stress Biomarkers; lipid Peroxidation