title: Comparative proteomic analysis provides new insights into cadmium accumulation in rice grain under cadmium stress.
abstract: Rice is one of the most important staple crops. During the growth season, rice plants are inevitably subjected to numerous stresses, among which heavy metal stress represented by cadmium contamination not only hindering the yield of rice but also affecting the food safety by Cd accumulating in rice grains. The mechanism of Cd accumulation in rice grains has not been well elucidated. In this study, we compare the proteomic difference between two genotypes with different Cd accumulation ability in grains. Verification of differentially expressed protein-encoding genes was analyzing by quantitative PCR (QPCR) and reanalysis of microarray expression data. Forty-seven proteins in total were successfully identified through proteomic screening. GO and KEGG enrichment analysis showed Cd accumulation triggered stress-related pathways in the cells, and strongly affecting metabolic pathways. Many proteins associated with nutrient reservoir and starch-related enzyme were identified in this study suggesting that a considerably damage on grain quality was caused. The results also implied stress response was initiated by the abnormal cells and the transmission of signals may mediated by reactive oxygen species (ROS). Our research will provide new insights into Cd accumulation in rice grain under Cd stress.
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China. Electronic address: dwxue@hznu.edu.cn.
authors.forename: Dawei
authors.initials: D
authors.lastname: Xue
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Xiangxiong
authors.initials: X
authors.lastname: Deng
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Xiangbin
authors.initials: X
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China.
authors.forename: Dali
authors.initials: D
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China. Electronic address: guolongbiao@caas.cn.
authors.forename: Longbiao
authors.initials: L
authors.lastname: Guo
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China. Electronic address: qianqian188@hotmail.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Qian
mesh_terms: D002104:Cadmium / Q000378:metabolism*; D020869:Gene Expression Profiling; D005838:Genotype; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D040901:Proteomics; D013312:Stress, Physiological
chemical_list: D002104:Cadmium
keywords: Cadmium; Gene chip; Proteomic; Quantitative PCR; Rice