title: Genomic characterization of a rotavirus G8P[1] detected in a child with diarrhea reveal direct animal-to-human transmission.
abstract: Group A rotavirus is a major cause of severe gastroenteritis in children and young animals. During a retrospective analysis of samples collected from Paraguayan children under 5 years old with diarrhea, and previously negative for rotavirus and norovirus, we detected the presence of bovine rotavirus sequences by viral metagenomics. Nucleic acid was extracted direct from stool sample and determined to be G8P[1]. The genomic analyzes revealed that the strain presents an Artiodactyl-like genome (G8-P[1]-I2-R2-C2-M1-Ax-N2-T6-E12-H3) suggesting a direct animal-to-human transmission.
authors.affiliation: Departamento de Biología Molecular y Genética, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Asunción, Paraguay. Electronic address: magaly.martinez@gmail.com.
authors.forename: Magaly
authors.initials: M
authors.lastname: Martinez
authors.affiliation: Blood Systems Research Institute, San Francisco, CA, USA.
authors.forename: Tung Gia
authors.initials: TG
authors.lastname: Phan
authors.affiliation: Departamento de Biología Molecular y Genética, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Asunción, Paraguay.
authors.forename: Maria Eugenia
authors.initials: ME
authors.lastname: Galeano
authors.affiliation: Departamento de Biología Molecular y Genética, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Asunción, Paraguay.
authors.forename: Graciela
authors.initials: G
authors.lastname: Russomando
authors.affiliation: Virology Institute, INTA Castelar, Buenos Aires, Argentina.
authors.forename: Viviana
authors.initials: V
authors.lastname: Parreno
authors.affiliation: Blood Systems Research Institute, San Francisco, CA, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Delwart
authors.affiliation: Departamento de Biología Molecular y Genética, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Asunción, Paraguay. Electronic address: gabriel_parra@hotmail.com.
authors.forename: Gabriel I
authors.initials: GI
authors.lastname: Parra
mesh_terms: D000818:Animals; D002675:Child, Preschool; D003967:Diarrhea / Q000821:virology*; D005759:Gastroenteritis / Q000821:virology; D005814:Genes, Viral; D016679:Genome, Viral; D023281:Genomics; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D010802:Phylogeny; D012189:Retrospective Studies; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D012400:Rotavirus Infections / Q000635:transmission* / Q000821:virology*
keywords: Bovine-like rotavirus G8P[1]; Interspecies transmission; Rotavirus