title: Establishment and partial characterization of an epirubicin-resistant gastric cancer cell line with upregulated ABCB1.
abstract: Multidrug resistance (MDR) is a major impediment to successful chemotherapy of gastric cancer. Our aim was to establish an epirubicin-resistant cell subline (AGS/EPI) and to elucidate the mechanisms involved in acquired EPI resistance. The AGS/EPI cell subline developed by exposing parental AGS cells to stepwise increasing concentrations of EPI demonstrated 2.52-fold resistance relative to the AGS cell line, and mRNA expression of the ATP-dependent drug-efflux pump P-glycoprotein (Pgp), more recently known as ABCB1 protein, was similarly upregulated. An AGS/EPI cell subline could thus be effectively established, and MDR mechanism of these cells was shown to be related to the overexpression of mRNA of the ABCB1 gene.
authors.affiliation: Department of Medicine, Gastroenterology Division, Federal University, Sao Paulo, Brazil E-mail : aledson.felipe@gmail.com.
authors.forename: Aledson Vitor
authors.initials: AV
authors.lastname: Felipe
authors.forename: Andrea Aparecida
authors.initials: AA
authors.lastname: Moraes
authors.forename: Juliana
authors.initials: J
authors.lastname: de Oliveira
authors.forename: Tiago Donizetti
authors.initials: TD
authors.lastname: da Silva
authors.forename: Nora Manoukian
authors.initials: NM
authors.lastname: Forones
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000096:biosynthesis / Q000235:genetics; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D018432:Drug Resistance, Multiple / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D015251:Epirubicin / Q000494:pharmacology*; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000235:genetics
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D000903:Antibiotics, Antineoplastic; D012333:RNA, Messenger; D015251:Epirubicin