title: Aldara-induced psoriasis-like skin inflammation: isolation and characterization of cutaneous dendritic cells and innate lymphocytes.
abstract: Psoriasis is a chronic auto-inflammatory skin disease of unknown etiology affecting millions of people worldwide. Dissecting the cellular networks and molecular signals promoting the development of psoriasis critically depends on appropriate animal models. Topical application of Aldara cream containing the Toll-like receptor (TLR)7-ligand Imiquimod induces skin inflammation and pathology in mice closely resembling plaque-type psoriasis in humans. The particular power of the Aldara model lies in examining the early events during psoriatic plaque formation, which is difficult to achieve in patients. Hence, recent reports using this model have challenged currently prevailing concepts concerning the pathophysiology of psoriasis. Here, we describe the induction and phenotype of Aldara-mediated dermatitis in mice and, in particular, analysis of the inflammatory cell infiltrate using flow cytometry.
authors.affiliation: Department of Immunology, Erasmus MC, University Medical Center Rotterdam, 3015 GE, Rotterdam, The Netherlands.
authors.forename: C T
authors.initials: CT
authors.lastname: Wohn
authors.forename: S
authors.initials: S
authors.lastname: Pantelyushin
authors.forename: J L
authors.initials: JL
authors.lastname: Ober-Blöbaum
authors.forename: B E
authors.initials: BE
authors.lastname: Clausen
mesh_terms: D000634:Aminoquinolines / Q000009:adverse effects*; D000818:Animals; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D006224:Cricetinae; D003713:Dendritic Cells / Q000473:pathology*; D003875:Drug Eruptions / Q000276:immunology* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D000077271:Imiquimod; D007113:Immunity, Innate; D008214:Lymphocytes / Q000473:pathology*; D008297:Male; D051379:Mice; D011565:Psoriasis / Q000139:chemically induced* / Q000276:immunology* / Q000473:pathology; D051381:Rats; D012867:Skin / Q000276:immunology / Q000473:pathology*
chemical_list: D000634:Aminoquinolines; D000077271:Imiquimod