title: Comparative studies of salinomycin-loaded nanoparticles prepared by nanoprecipitation and single emulsion method.
abstract: To establish a satisfactory delivery system for the delivery of salinomycin (Sal), a novel, selective cancer stem cell inhibitor with prominent toxicity, gelatinase-responsive core-shell nanoparticles (NPs), were prepared by nanoprecipitation method (NR-NPs) and single emulsion method (SE-NPs). The gelatinase-responsive copolymer was prepared by carboxylation and double amination method. We studied the stability of NPs prepared by nanoprecipitation method with different proportions of F68 in aqueous phase to determine the best proportion used in our study. Then, the NPs were prepared by nanoprecipitation method with the best proportion of F68 and single emulsion method, and their physiochemical traits including morphology, particle size, zeta potential, drug loading content, stability, and in vitro release profiles were studied. The SE-NPs showed significant differences in particle size, drug loading content, stability, and in vitro release profiles compared to NR-NPs. The SE-NPs presented higher drug entrapment efficiency and superior stability than the NR-NPs. The drug release rate of SE-NPs was more sustainable than that of the NR-NPs, and in vivo experiment indicated that NPs could prominently reduce the toxicity of Sal. Our study demonstrates that the SE-NPs could be a satisfactory method for the preparation of gelatinase-responsive NPs for intelligent delivery of Sal.
authors.affiliation: Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, 321 Zhongshan Road, Nanjing 210008, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: The Comprehensive Cancer Center of Drum Tower Hospital, Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University, Zhongshan Road 321, Nanjing 210008, People's Republic of China.
authors.forename: Puyuan
authors.initials: P
authors.lastname: Wu
authors.affiliation: The Comprehensive Cancer Center of Drum Tower Hospital, Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University, Zhongshan Road 321, Nanjing 210008, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ren
authors.affiliation: Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, 321 Zhongshan Road, Nanjing 210008, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Xin
authors.affiliation: The Comprehensive Cancer Center of Drum Tower Hospital, Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University, Zhongshan Road 321, Nanjing 210008, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Laboratory of Mesoscopic Chemistry and Department of Polymer Science and Engineering, College of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing 210093, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Xie
authors.affiliation: Laboratory of Mesoscopic Chemistry and Department of Polymer Science and Engineering, College of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing 210093, People's Republic of China.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Yang
authors.affiliation: The Comprehensive Cancer Center of Drum Tower Hospital, Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University, Zhongshan Road 321, Nanjing 210008, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
authors.affiliation: The Comprehensive Cancer Center of Drum Tower Hospital, Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University, Zhongshan Road 321, Nanjing 210008, People's Republic of China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Yu
authors.affiliation: Laboratory of Mesoscopic Chemistry and Department of Polymer Science and Engineering, College of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing 210093, People's Republic of China.
authors.forename: Xiqun
authors.initials: X
authors.lastname: Jiang
authors.affiliation: The Comprehensive Cancer Center of Drum Tower Hospital, Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University, Zhongshan Road 321, Nanjing 210008, People's Republic of China.
authors.forename: Baorui
authors.initials: B
authors.lastname: Liu
authors.affiliation: The Comprehensive Cancer Center of Drum Tower Hospital, Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University, Zhongshan Road 321, Nanjing 210008, People's Republic of China.
authors.forename: Rutain
authors.initials: R
authors.lastname: Li
authors.affiliation: The Comprehensive Cancer Center of Drum Tower Hospital, Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University, Zhongshan Road 321, Nanjing 210008, People's Republic of China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Wang
keywords: Drug delivery; Gelatinase; Nanoparticles; Nanoprecipitation method; Salinomycin; Single emulsion method