title: Encapsulation of adenovirus serotype 5 in anionic lecithin liposomes using a bead-based immunoprecipitation technique enhances transfection efficiency.
abstract: Oncolytic viruses (OVs) constitute a promising class of cancer therapeutics which exploit validated genetic pathways known to be deregulated in many cancers. To overcome an immune response and to enhance its potential use to treat primary and metastatic tumors, a method for liposomal encapsulation of adenovirus has been developed. The encapsulation of adenovirus in non-toxic anionic lecithin-cholesterol-PEG liposomes ranging from 140 to 180 nm in diameter have been prepared by self-assembly around the viral capsid. The encapsulated viruses retain their ability to infect cancer cells. Furthermore, an immunoprecipitation (IP) technique has shown to be a fast and effective method to extract non-encapsulated viruses and homogenize the liposomes remaining in solution. 78% of adenovirus plaque forming units were encapsulated and retained infectivity after IP processing. Additionally, encapsulated viruses have shown enhanced transfection efficiency up to 4 × higher compared to non-encapsulated Ads. Extracting non-encapsulated viruses from solution may prevent an adverse in vivo immune response and may enhance treatment for multiple administrations.
authors.affiliation: Department of Materials Science and Engineering, University of California, San Diego (UCSD), La Jolla, CA, USA.
authors.forename: Natalie
authors.initials: N
authors.lastname: Mendez
authors.affiliation: Department of Bioengineering, UCSD, La Jolla, CA, USA.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Herrera
authors.affiliation: Moores Cancer Center, UCSD, La Jolla, CA, USA.
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Moores Cancer Center, UCSD, La Jolla, CA, USA.
authors.forename: Farah
authors.initials: F
authors.lastname: Hedjran
authors.affiliation: Department of Biology, San Diego State University (SDSU), San Diego, CA, USA.
authors.forename: Ralph
authors.initials: R
authors.lastname: Feuer
authors.affiliation: Department of Medicine, UCSD, La Jolla, CA, USA.
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Blair
authors.affiliation: Department of Chemistry and Biochemistry, UCSD, La Jolla, CA, USA.
authors.forename: William C
authors.initials: WC
authors.lastname: Trogler
authors.affiliation: Department of Medicine, UCSD, La Jolla, CA, USA.
authors.forename: Tony R
authors.initials: TR
authors.lastname: Reid
authors.affiliation: Department of Chemistry and Biochemistry, UCSD, La Jolla, CA, USA. Electronic address: akummel@ucsd.edu.
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Kummel
mesh_terms: D000256:Adenoviridae / Q000145:classification / Q000302:isolation & purification*; D000818:Animals; D045744:Cell Line, Tumor; D015316:Genetic Therapy / Q000379:methods; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation; D054709:Lecithins / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D051379:Mice; D057507:Mice, 129 Strain; D050130:Oncolytic Virotherapy; D014162:Transfection
chemical_list: D054709:Lecithins; D008081:Liposomes
keywords: Adenovirus; Drug delivery; Gene therapy; Liposome; Nanoparticle; Phospholipid