title: Mechanism of motilin-mediated inhibition on voltage-dependent potassium currents in hippocampal neurons.
abstract: OBJECTIVE
  The effects of motilin on voltage-dependent K+ currents in hippocampal neurons with the addition of L-arginine (L-AA), D-arginine (D-AA) and N-nitro-L-arginine methyl ester (L-NAME) were investigated in this study.
  
  
  METHODS
  Mice (1-3 days old) were randomly assigned to different groups according to the addition of motilin, L-AA, D-AA, and L-NAME. The K+ current signals were detected by the whole-cell patch-clamp technique.
  
  
  RESULTS
  Compared with the control group, the transient outward voltage-dependent K+ current was significantly inhibited by motilin added with L-AA. In contrast, the addition of motilin and L-NAME significantly increased the K+ current, while no significant change was detected by the addition of motilin accompanied with D-AA.
  
  
  CONCLUSIONS
  The inhibiting effects of motilin on the voltage-dependent K+ current in hippocampal neurons indicate that motilin acts as a regulatory factor for the nitric oxide pathway.
authors.affiliation: Department of Neonatology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi province 710061, China; Department of Physiology, Heze Medical College, Heze, Shandong Province, China. Electronic address: luyong20306@163.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Stomatology, Medical College of Qingdao University, Qingdao, Shandong Province 266003, China. Electronic address: rayratlily@yahoo.com.
authors.forename: F
authors.initials: F
authors.lastname: Zhong
authors.affiliation: Department of Physiology, Heze Medical College, Heze, Shandong Province, China. Electronic address: 928734056@qq.com.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Neonatology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi province 710061, China. Electronic address: huili@mail.xjtu.edu.cn.
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Neonatology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi province 710061, China; Shaanxi Province Biomedicine Key Laboratory, College of Life Sciences, Northwest University, Xi'an, Shanxi province, China. Electronic address: zlzhu@mail.xjtu.edu.cn.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of Physiology, Heze Medical College, Heze, Shandong Province, China. Electronic address: hzyzkxx@163.com.
authors.forename: X
authors.initials: X
authors.lastname: Kong
authors.affiliation: Department of Physiology, Heze Medical College, Heze, Shandong Province, China. Electronic address: hzyzzjk@163.com.
authors.forename: J
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Physiology, Heze Medical College, Heze, Shandong Province, China. Electronic address: 77182453@qq.com.
authors.forename: Q
authors.initials: Q
authors.lastname: Wu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D002478:Cells, Cultured; D006624:Hippocampus / Q000187:drug effects* / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D049990:Membrane Transport Modulators / Q000494:pharmacology*; D009037:Motilin / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000378:metabolism; D024642:Potassium Channels, Voltage-Gated / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011897:Random Allocation; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D049990:Membrane Transport Modulators; D024642:Potassium Channels, Voltage-Gated; C026512:arginine methyl ester; D009569:Nitric Oxide; D009037:Motilin; D001120:Arginine; D011188:Potassium
keywords: hippocampal neurons; motilin; nitric oxide; whole-cell patch clamp