title: Antivenomic characterization of two antivenoms against the venom of the taipan, Oxyuranus scutellatus, from Papua New Guinea and Australia.
abstract: Antivenoms manufactured by bioCSL Limited (Australia) and Instituto Clodomiro Picado (Costa Rica) against the venom of the taipan snakes (Oxyuranus scutellatus) from Australia and Papua New Guinea (PNG), respectively, were compared using antivenomics, an analytical approach that combines proteomics with immunoaffinity chromatography. Both antivenoms recognized all venom proteins present in venom from PNG O. scutellatus, although a pattern of partial recognition was observed for some components. In the case of the Australian O. scutellatus venom, both antivenoms immunorecognized the majority of the components, but the CSL antivenom showed a stronger pattern of immunoreactivity, which was revealed by the percentage of retained proteins in the immunoaffinity column. Antivenoms interacted with taipoxin in surface plasmon resonance. These observations on antivenomics agree with previous neutralization studies.
authors.affiliation: Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica; Charles Campbell Toxinology Centre, School of Medicine and Health Sciences, University of Papua New Guinea, Port Moresby, Papua New Guinea; Special Laboratory of Applied Toxinology, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, Sao Paulo, Brazil; Australian Venom Research Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: María
authors.initials: M
authors.lastname: Herrera
authors.affiliation: Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica; Charles Campbell Toxinology Centre, School of Medicine and Health Sciences, University of Papua New Guinea, Port Moresby, Papua New Guinea; Special Laboratory of Applied Toxinology, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, Sao Paulo, Brazil; Australian Venom Research Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Owen K
authors.initials: OK
authors.lastname: Paiva
authors.affiliation: Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica; Charles Campbell Toxinology Centre, School of Medicine and Health Sciences, University of Papua New Guinea, Port Moresby, Papua New Guinea; Special Laboratory of Applied Toxinology, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, Sao Paulo, Brazil; Australian Venom Research Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Ana Helena
authors.initials: AH
authors.lastname: Pagotto
authors.affiliation: Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica; Charles Campbell Toxinology Centre, School of Medicine and Health Sciences, University of Papua New Guinea, Port Moresby, Papua New Guinea; Special Laboratory of Applied Toxinology, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, Sao Paulo, Brazil; Australian Venom Research Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Segura
authors.affiliation: Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica; Charles Campbell Toxinology Centre, School of Medicine and Health Sciences, University of Papua New Guinea, Port Moresby, Papua New Guinea; Special Laboratory of Applied Toxinology, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, Sao Paulo, Brazil; Australian Venom Research Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Solange M T
authors.initials: SM
authors.lastname: Serrano
authors.affiliation: Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica; Charles Campbell Toxinology Centre, School of Medicine and Health Sciences, University of Papua New Guinea, Port Moresby, Papua New Guinea; Special Laboratory of Applied Toxinology, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, Sao Paulo, Brazil; Australian Venom Research Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Mariángela
authors.initials: M
authors.lastname: Vargas
authors.affiliation: Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica; Charles Campbell Toxinology Centre, School of Medicine and Health Sciences, University of Papua New Guinea, Port Moresby, Papua New Guinea; Special Laboratory of Applied Toxinology, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, Sao Paulo, Brazil; Australian Venom Research Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Mauren
authors.initials: M
authors.lastname: Villalta
authors.affiliation: Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica; Charles Campbell Toxinology Centre, School of Medicine and Health Sciences, University of Papua New Guinea, Port Moresby, Papua New Guinea; Special Laboratory of Applied Toxinology, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, Sao Paulo, Brazil; Australian Venom Research Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Simon D
authors.initials: SD
authors.lastname: Jensen
authors.affiliation: Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica; Charles Campbell Toxinology Centre, School of Medicine and Health Sciences, University of Papua New Guinea, Port Moresby, Papua New Guinea; Special Laboratory of Applied Toxinology, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, Sao Paulo, Brazil; Australian Venom Research Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Guillermo
authors.initials: G
authors.lastname: León
authors.affiliation: Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica; Charles Campbell Toxinology Centre, School of Medicine and Health Sciences, University of Papua New Guinea, Port Moresby, Papua New Guinea; Special Laboratory of Applied Toxinology, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, Sao Paulo, Brazil; Australian Venom Research Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: David J
authors.initials: DJ
authors.lastname: Williams
authors.affiliation: Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica; Charles Campbell Toxinology Centre, School of Medicine and Health Sciences, University of Papua New Guinea, Port Moresby, Papua New Guinea; Special Laboratory of Applied Toxinology, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, Sao Paulo, Brazil; Australian Venom Research Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, Victoria, Australia jose.gutierrez@ucr.ac.cr.
authors.forename: José María
authors.initials: JM
authors.lastname: Gutiérrez
mesh_terms: D000818:Animals; D000997:Antivenins / Q000737:chemistry* / Q000494:pharmacology*; D001315:Australia; D003364:Costa Rica; D004546:Elapid Venoms / Q000037:antagonists & inhibitors / Q000633:toxicity*; D017815:Elapidae; D009500:Neutralization Tests; D010219:Papua New Guinea; D040901:Proteomics; D020349:Surface Plasmon Resonance
chemical_list: D000997:Antivenins; D004546:Elapid Venoms; C015523:taipoxin