title: Survey of the transcriptome of Brevibacillus borstelensis exposed to low temperature shock.
abstract: Molecular mechanisms underlying the ability of Brevibacillus borstelensis to survive and adapt to various environmentally relevant stresses are poorly understood. To define organism's molecular response to low temperature, gene expression profile of B. borstelensis at 20 °C was carried out by high-throughput sequencing technology. A total of 4579 transcripts with a maximum transcript length of 9919 bp were annotated. Gene expression profiling identified 712 genes that were significantly up- or down-regulated during cold shock. Functional categorization of the differentially expressed genes revealed that response to stress, regulation of transcription, transport, signal transduction and cytoplasm were the differentially regulated processes. The microbial stress responsive genes (hsp90, hslU, grpE, dnaK, dnaJ, hslV) and genes under regulatory adaptive responses (rpoN) were identified. The gene encoding cold shock protein purine nucleoside phosphorylase was found to be remarkably up-regulated. RT-PCR experiments carried out on genes expressed under cold shock independently verified the transcriptome data results. In addition, a large number of genes encoding hypothetical protein were identified. The brief survey of the transcripts obtained in response to cold shock underlines the survival strategy of thermophilic bacteria exposed to low temperature environment, which is further helpful in generating genetic information associated with this bacteria.
authors.affiliation: Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Orissa, India.
authors.forename: S
authors.initials: S
authors.lastname: Tripathy
authors.affiliation: Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Orissa, India.
authors.forename: R
authors.initials: R
authors.lastname: Sen
authors.affiliation: Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Orissa, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Padhi
authors.affiliation: Division of Fish Genetics and Biotechnology, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Orissa, India.
authors.forename: D K
authors.initials: DK
authors.lastname: Sahu
authors.affiliation: Division of Fish Genetics and Biotechnology, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Orissa, India.
authors.forename: S
authors.initials: S
authors.lastname: Nandi
authors.affiliation: Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Orissa, India.
authors.forename: S
authors.initials: S
authors.lastname: Mohanty
authors.affiliation: Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Orissa, India. Electronic address: maitink@yahoo.co.in.
authors.forename: N K
authors.initials: NK
authors.lastname: Maiti
mesh_terms: D058675:Brevibacillus / Q000235:genetics*; D003080:Cold Temperature; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D059014:High-Throughput Nucleotide Sequencing; D058977:Molecular Sequence Annotation; D017423:Sequence Analysis, RNA; D013312:Stress, Physiological / Q000235:genetics*; D059467:Transcriptome
keywords: Brevibacillus borstelensis; Low temperature; Thermophile; Transcriptome