title: PLCβ1a and PLCβ1b selective regulation and cyclin D3 modulation reduced by kinamycin F during k562 cell differentiation.
abstract: Here we report that both PLCβ1a and PLCβ1b are relevant regulators of erythropoiesis in that kinamycin F, a potent inducer of γ-globin production in K562 cells, caused a selectively reduction of both PLCβ1 isozymes even though the results point out that the effect of the drug is mainly directed toward the expression of the PLCβ1a isoform. We have identified a different role for the two isozymes as regulators of K562 differentiation process induced by kinamycin F. The overexpression of PLCβ1b induced an increase in γ-globin expression even in the absence of kinamycin F. Moreover during K562 differentiation, cyclin D3 level is regulated by PLCβ1 signaling pathway. Namely the amplification of the expression of the PLCβ1a, but not of PLCβ1b, is able to maintain high levels of expression of cyclin D3 even after treatment with kinamycin F. This could be due to their different distribution in the cell compartments since the amount of PLCβ1b is mainly present in the nucleus in respect to PLCβ1a. Our data indicate that the amplification of PLCβ1a expression, following treatment with kinamycin F, confers a real advantage to K562 cells viability and protects cells themselves from apoptosis.
authors.affiliation: SC Laboratory of Musculoskeletal Cell Biology, Rizzoli Orthopedic Institute, Bologna, Italy; Laboratory RAMSES, Rizzoli Orthopedic Institute, Bologna, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Bavelloni
authors.forename: Gary I
authors.initials: GI
authors.lastname: Dmitrienko
authors.forename: Valerie J
authors.initials: VJ
authors.lastname: Goodfellow
authors.forename: Ahmad
authors.initials: A
authors.lastname: Ghavami
authors.forename: Manuela
authors.initials: M
authors.lastname: Piazzi
authors.forename: William
authors.initials: W
authors.lastname: Blalock
authors.forename: Francesca
authors.initials: F
authors.lastname: Chiarini
authors.forename: Lucio
authors.initials: L
authors.lastname: Cocco
authors.forename: Irene
authors.initials: I
authors.lastname: Faenza
mesh_terms: D017209:Apoptosis; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D056743:Cyclin D3 / Q000096:biosynthesis / Q000235:genetics*; D004920:Erythropoiesis / Q000187:drug effects / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006801:Humans; D054799:Phospholipase C beta / Q000096:biosynthesis*; D020033:Protein Isoforms / Q000096:biosynthesis* / Q000235:genetics; D011809:Quinones / Q000008:administration & dosage; D015398:Signal Transduction / Q000187:drug effects; D055545:gamma-Globins / Q000096:biosynthesis*; D000095602:Angucyclines and Angucyclinones
chemical_list: D056743:Cyclin D3; D054799:Phospholipase C beta; D020033:Protein Isoforms; D011809:Quinones; D055545:gamma-Globins; C059021:kinamycin F; D000095602:Angucyclines and Angucyclinones; C518033:PLCB1 protein, human