title: Dynamic analysis of CO₂ labeling and cell respiration using membrane-inlet mass spectrometry.
abstract: Here, we introduce a mass spectrometry-based analytical method and relevant technical details for dynamic cell respiration and CO2 labeling analysis. Such measurements can be utilized as additional information and constraints for model-based (13)C metabolic flux analysis. Dissolved dynamics of oxygen consumption and CO2 mass isotopomer evolution from (13)C-labeled tracer substrates through different cellular processes can be precisely measured on-line using a miniaturized reactor system equipped with a membrane-inlet mass spectrometer. The corresponding specific rates of physiologically relevant gases and CO2 mass isotopomers can be quantified within a short-term range based on the liquid-phase dynamics of dissolved fermentation gases.
authors.affiliation: Genomatica Inc., 4757 Nexus Center Drive, San Diego, CA, 92121, USA, tyang@genomatica.com.
authors.forename: Tae Hoon
authors.initials: TH
authors.lastname: Yang
mesh_terms: D002245:Carbon Dioxide / Q000737:chemistry*; D002247:Carbon Isotopes; D019069:Cell Respiration / Q000502:physiology*; D007553:Isotope Labeling / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods*; D064688:Metabolic Flux Analysis / Q000379:methods*; D008954:Models, Biological; D010101:Oxygen Consumption / Q000502:physiology*
chemical_list: D002247:Carbon Isotopes; D002245:Carbon Dioxide