title: Characterization of potent odorants in male giant water bug (Lethocerus indicus Lep. and Serv.), an important edible insect of Southeast Asia.
abstract: Potent odorants in frozen fresh (FFB) and salted boiled (SBB) male giant water bugs (Lethocerus indicus), or 'Maengdana' in Thai, were characterized by application of direct solvent extraction/solvent-assisted flavour evaporation (SAFE), gas chromatography-mass spectrometry (GC-MS), gas chromatography-olfactometry (GC-O), aroma extract dilution analysis (AEDA) and stable isotope dilution assays (SIDA). Twenty and 27 potent odorants were detected in FFB and SBB, respectively. Most odorants were lipid-derived compounds, including the two most abundant volatile components (E)-2-hexenyl acetate and (E)-2-hexenyl butanoate, which contributed banana-like odours. 2-Acetyl-1-pyrroline and 2-acetyl-2-thiazoline, responsible for popcorn-like odours, were detected in SBB only. An aroma reconstitution model of SBB was constructed in an oil-in-water emulsion matrix using 12 selected potent odorants based on the results of AEDA, accurate compound quantification and the calculated odour-activity values (OAV). Omission studies were carried out to verify the significance of esters, particularly (E)-2-hexenyl acetate was determined to be an important character-impact odorant in male giant water bug aroma.
authors.affiliation: School of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Nakhon Ratchasima 30000, Thailand.
authors.forename: Patthamawadi
authors.initials: P
authors.lastname: Kiatbenjakul
authors.affiliation: School of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Nakhon Ratchasima 30000, Thailand. Electronic address: ikanok-orn@sut.ac.th.
authors.forename: Kanok-Orn
authors.initials: KO
authors.lastname: Intarapichet
authors.affiliation: Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, 1302 West Pennsylvania Avenue, Urbana, IL 61801, USA.
authors.forename: Keith R
authors.initials: KR
authors.lastname: Cadwallader
mesh_terms: D000818:Animals; D001210:Asia, Southeastern; D008401:Gas Chromatography-Mass Spectrometry; D006430:Hemiptera / Q000737:chemistry* / Q000378:metabolism; D059625:Liquid-Liquid Extraction; D008297:Male; D009812:Odorants / Q000032:analysis*; D009821:Oils / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D009821:Oils; D014867:Water
keywords: Aroma extract dilution analysis; Aroma reconstitution model; Edible insect; Gas chromatography-olfactometry; Gas chromatography–mass spectrometry; Giant water bug (Lethocerus indicus); Odour activity value; Omission studies