title: Investigation of two-dimensional high performance liquid chromatography approaches for reversed phase resolution of warfarin and hydroxywarfarin isomers.
abstract: Several offline and online 2D HPLC methods were investigated for the reversed phase resolution of a complex mixture of closely related warfarin and hydroxywarfarin isomers. By combining reversed phase achiral/chiral HPLC separation with UV-triggered fraction collection and subsequent chiral/achiral reversed phase HPLC analysis of collected fractions, complete resolution of all 12 components of the mixture was possible. In addition, a faster method was developed from online 2D HPLC analysis where multicomponent fractions from the first dimension are simultaneously chromatographed in the second dimension.
authors.affiliation: Merck Research Laboratories, Rahway, NJ 07065, USA. Electronic address: erik.regalado@merck.com.
authors.forename: Erik L
authors.initials: EL
authors.lastname: Regalado
authors.affiliation: Merck Research Laboratories, Rahway, NJ 07065, USA.
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Schariter
authors.affiliation: Merck Research Laboratories, Rahway, NJ 07065, USA. Electronic address: christopher_welch@merck.com.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Welch
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007536:Isomerism; D014859:Warfarin / Q000032:analysis* / Q000737:chemistry
chemical_list: D014859:Warfarin
keywords: Chiral separations; Closely related species; Complex mixtures; Method development; Two-dimensional HPLC; Warfarin