title: Production of thermostable lipase by Thermomyces lanuginosus on solid-state fermentation: selective hydrolysis of sardine oil.
abstract: A naturally immobilized biocatalyst with lipase activity was produced by Thermomyces lanuginosus on solid-state fermentation with perlite as inert support. Maxima lipase activities (22 and 120 U/g of dry matter, using p-nitrophenyl octanoate and trioctanoine, respectively, as substrates) were obtained after 72 h of solid culture, remaining nearly constant up to 120 h. Maxima lipase activity was found at 60 to 85 °C and pH 10. The biocatalyst was stable at 60 °C for at least 4 h of incubation and a pH from 7 to 10. Energy values of activation and deactivation of lipase were of 26 and 6.7 kJ/mol, respectively. The biocatalyst shows high selectivity for the release of the omega-3 polyunsaturated fatty acids, eicosapentaenoic (EPA) and docosahexaenoic acids (DHA), during the hydrolysis of sardine oil. The EPA/DHA ratio (16:6) released by this biocatalyst was superior to that obtained with the commercial preparations of T. lanuginosus.
authors.affiliation: Universidad Autónoma Metropolitana-Unidad Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, Iztapalapa, Mexico, 09340, D.F., Mexico.
authors.forename: Nayeli
authors.initials: N
authors.lastname: Avila-Cisneros
authors.forename: Susana
authors.initials: S
authors.lastname: Velasco-Lozano
authors.forename: Sergio
authors.initials: S
authors.lastname: Huerta-Ochoa
authors.forename: Jesús
authors.initials: J
authors.lastname: Córdova-López
authors.forename: Miquel
authors.initials: M
authors.lastname: Gimeno
authors.forename: Ernesto
authors.initials: E
authors.lastname: Favela-Torres
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000201:enzymology*; D002384:Catalysis; D004789:Enzyme Activation; D004795:Enzyme Stability; D005227:Fatty Acids / Q000378:metabolism*; D005395:Fish Oils / Q000382:microbiology*; D005399:Fishes / Q000378:metabolism*; D006868:Hydrolysis; D008049:Lipase / Q000096:biosynthesis*; D013696:Temperature
chemical_list: D005227:Fatty Acids; D005395:Fish Oils; D008049:Lipase