title: The utilization of a new immunochromatographic test in detection of Helicobacter pylori antibody from maternal and umbilical cord serum.
abstract: BACKGROUND
  Helicobacter pylori (H. pylori) was linked with several extragastrointestinal diseases, including preeclampsia and intrauterine growth restriction of fetus. One of the signals which can be transferred from mother to fetus is the H. pylori IgG antibody. Aims. We utilized a commercial immunochromatographic kit to detect the antibody in maternal and cord serum.
  
  
  METHODS
  Three hundred and forty-six females were enrolled and the blood samples were collected on antenatal examination and on delivery. The maternal H. pylori infection was determined by stool H. pylori antigen test.
  
  
  RESULTS
  One hundred and five females (30.3%) were H. pylori-infected, and the prevalence was higher in immigrants (43.5%) than in Taiwanese (28.7%, P = 0.058). The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the kit were 77.1%, 88.0%, 73.6%, 89.8%, and 84.7%, respectively. This kit also had similar performance in cord serum. Comparing to the maternal result on delivery, this kit offered a consistent performance in antenatal maternal serum (kappa coefficient 0.92) and in cord serum (kappa coefficient 0.88).
  
  
  CONCLUSIONS
  H. pylori IgG antibody can be transferred through the placenta into the fetal circulation. However, accuracy of the test kit needs to be evaluated before utilization in screening.
authors.affiliation: Graduate Institute of Public Health, Kaohsiung Medical University, Kaohsiung 807, Taiwan ; Department of Gynecology and Obstetrics, E-Da Hospital, Kaohsiung 824, Taiwan ; School of Medicine, College of Medicine, I-Shou University, Kaohsiung 824, Taiwan.
authors.forename: Fu-Chen
authors.initials: FC
authors.lastname: Kuo
authors.affiliation: Department of Pediatrics, E-Da Hospital, I-Shou University, Kaohsiung 824, Taiwan.
authors.forename: Chien-Yi
authors.identifier: 0000-0001-9162-7359
authors.initials: CY
authors.lastname: Wu
authors.affiliation: Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan ; Department of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Chao-Hung
authors.identifier: 0000-0003-1847-395X
authors.initials: CH
authors.lastname: Kuo
authors.affiliation: Graduate Institute of Public Health, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Chia-Fang
authors.initials: CF
authors.lastname: Wu
authors.affiliation: Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan ; Department of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Chien-Yu
authors.initials: CY
authors.lastname: Lu
authors.affiliation: Department of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan ; Division of Infectious Diseases, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
authors.forename: Yen-Hsu
authors.identifier: 0000-0001-5012-1300
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Department of Radiology, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan ; Department of Medical Imaging, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
authors.forename: Chiao-Yun
authors.initials: CY
authors.lastname: Chen
authors.affiliation: Department of Pharmacology, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Yi-Ching
authors.initials: YC
authors.lastname: Lo
authors.affiliation: Graduate Institute of Public Health, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Ming-Tsang
authors.initials: MT
authors.lastname: Wu
authors.affiliation: Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan ; Department of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Huang-Ming
authors.initials: HM
authors.lastname: Hu
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods*; D005260:Female; D005312:Fetal Blood / Q000276:immunology* / Q000382:microbiology; D016481:Helicobacter Infections / Q000097:blood* / Q000276:immunology; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D008431:Maternal-Fetal Exchange / Q000276:immunology*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000097:blood* / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial