title: Cell migration is another player of the minute virus of mice infection.
abstract: The parvovirus minute virus of mice, prototype strain (MVMp), preferentially infects and kills cancer cells. This intrinsic MVMp oncotropism may depend in part on the early stages of MVMp infection. To test this hypothesis, we investigated the early events of MVMp infection in mouse LA9 fibroblasts and a highly invasive mouse mammary tumor cell line derived from polyomavirus middle T antigen-mediated transformation. Using a combination of fluorescence and electron microscopy, we found that various parameters of the cell migration process affect MVMp infection. We show that, after binding to the plasma membrane, MVMp particles rapidly cluster at the leading edge of migrating cells, which exhibit higher levels of MVMp uptake than non-motile cells. Moreover, promoting cell migration on a fibronectin matrix increased MVMp infection, and induction of epithelial-mesenchymal transition allowed MVMp replication in non-permissive epithelial cells. Hence, we propose that cell migration influences the early stages of MVMp infection.
authors.affiliation: Department of Zoology, University of British Columbia, 6270 University Boulevard, Vancouver, British Columbia, Canada V6T 1Z4.
authors.forename: Pierre O
authors.initials: PO
authors.lastname: Garcin
authors.affiliation: Department of Zoology, University of British Columbia, 6270 University Boulevard, Vancouver, British Columbia, Canada V6T 1Z4. Electronic address: pante@zoology.ubc.ca.
authors.forename: Nelly
authors.initials: N
authors.lastname: Panté
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology / Q000821:virology; D058750:Epithelial-Mesenchymal Transition; D005353:Fibronectins; D051379:Mice; D008915:Minute Virus of Mice / Q000502:physiology*
chemical_list: D005353:Fibronectins
keywords: Cell migration; Cell protrusions; Epithelial–mesenchymal transition; Filopodia; MVMp early infection; Minute virus of mice; Viral oncotropism