title: Mitochondrial respiration and genomic analysis provide insight into the influence of the symbiotic bacterium on host trypanosomatid oxygen consumption.
abstract: Certain trypanosomatids co-evolve with an endosymbiotic bacterium in a mutualistic relationship that is characterized by intense metabolic exchanges. Symbionts were able to respire for up to 4 h after isolation from Angomonas deanei. FCCP (carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone) similarly increased respiration in wild-type and aposymbiotic protozoa, though a higher maximal O2 consumption capacity was observed in the symbiont-containing cells. Rotenone, a complex I inhibitor, did not affect A. deanei respiration, whereas TTFA (thenoyltrifluoroacetone), a complex II activity inhibitor, completely blocked respiration in both strains. Antimycin A and cyanide, inhibitors of complexes III and IV, respectively, abolished O2 consumption, but the aposymbiotic protozoa were more sensitive to both compounds. Oligomycin did not affect cell respiration, whereas carboxyatractyloside (CAT), an inhibitor of the ADP-ATP translocator, slightly reduced O2 consumption. In the A. deanei genome, sequences encoding most proteins of the respiratory chain are present. The symbiont genome lost part of the electron transport system (ETS), but complex I, a cytochrome d oxidase, and FoF1-ATP synthase remain. In conclusion, this work suggests that the symbiont influences the mitochondrial respiration of the host protozoan.
authors.affiliation: Laboratório de Ultraestrutura Celular Hertha Meyer,Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro,UFRJ, Avenida Carlos Chagas Filho, 343, Bloco G, Subsolo, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, CEP 21941-590,Brasil.
authors.forename: A C
authors.initials: AC
authors.lastname: Azevedo-Martins
authors.affiliation: Laboratório de Ultraestrutura Celular Hertha Meyer,Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro,UFRJ, Avenida Carlos Chagas Filho, 343, Bloco G, Subsolo, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, CEP 21941-590,Brasil.
authors.forename: A C L
authors.initials: AC
authors.lastname: Machado
authors.affiliation: BAMBOO Team,INRIA Grenoble-Rhône-Alpes,Villeurbanne,France.
authors.forename: C C
authors.initials: CC
authors.lastname: Klein
authors.affiliation: Laboratório Nacional de Computação Científica,Av. Getúlio Vargas, 333, Quitandinha, Petrópolis, RJ, CEP: 25651-075,Brasil.
authors.forename: L
authors.initials: L
authors.lastname: Ciapina
authors.affiliation: Laboratório Nacional de Computação Científica,Av. Getúlio Vargas, 333, Quitandinha, Petrópolis, RJ, CEP: 25651-075,Brasil.
authors.forename: L
authors.initials: L
authors.lastname: Gonzaga
authors.affiliation: Laboratório Nacional de Computação Científica,Av. Getúlio Vargas, 333, Quitandinha, Petrópolis, RJ, CEP: 25651-075,Brasil.
authors.forename: A T R
authors.initials: AT
authors.lastname: Vasconcelos
authors.affiliation: BAMBOO Team,INRIA Grenoble-Rhône-Alpes,Villeurbanne,France.
authors.forename: M F
authors.initials: MF
authors.lastname: Sagot
authors.affiliation: Laboratório de Ultraestrutura Celular Hertha Meyer,Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro,UFRJ, Avenida Carlos Chagas Filho, 343, Bloco G, Subsolo, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, CEP 21941-590,Brasil.
authors.forename: W
authors.initials: W
authors.lastname: DE Souza
authors.affiliation: Laboratório Intermediário de Biomembranas, Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde,Universidade Federal do Rio de Janeiro, UFRJ,Avenida Carlos Chagas Filho, 343, Bloco C, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, CEP 21941-590,Brasil.
authors.forename: M
authors.initials: M
authors.lastname: Einicker-Lamas
authors.affiliation: Laboratório de Bioenergética e Fisiologia Mitocondrial, Programa de Biofísica e Bioquímica Celular, Instituto de Bioquímica Médica, Centro de Ciências da Saúde,Universidade Federal do Rio de Janeiro, UFRJ,Avenida Carlos Chagas Filho, 343, Bloco D SS sala 13, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, CEP 21941-590,Brasil.
authors.forename: A
authors.initials: A
authors.lastname: Galina
authors.affiliation: Laboratório de Ultraestrutura Celular Hertha Meyer,Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro,UFRJ, Avenida Carlos Chagas Filho, 343, Bloco G, Subsolo, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, CEP 21941-590,Brasil.
authors.forename: M C M
authors.initials: MC
authors.lastname: Motta
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000378:metabolism; D005075:Biological Evolution; D004579:Electron Transport / Q000235:genetics / Q000502:physiology; D005786:Gene Expression Regulation; D008928:Mitochondria / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*; D013559:Symbiosis / Q000502:physiology*; D014351:Trypanosomatina / Q000235:genetics / Q000382:microbiology* / Q000502:physiology*
keywords: evolution