title: Mass cytometry analysis of human T cell phenotype and function.
abstract: Mass cytometry is a form of flow cytometry based on single-cell mass spectrometry that uses monoisotopic elemental labels to probe individual cells. Reduced cross talk between channels and an ability to measure >30 independent cellular parameters make this an attractive approach for high-dimensional analysis of cellular phenotypes and function. Here, methods of using this approach for the analysis of human T cell surface markers and intracellular cytokines are described.
authors.affiliation: Singapore Immunology Network (SIgN), Agency for Science Technology and Research (A-STAR), 8A Biomedical Grove, Immunos #03-06, Singapore, 138648, Singapore, evan_newell@immunol.a-star.edu.sg.
authors.forename: Evan W
authors.initials: EW
authors.lastname: Newell
authors.forename: Lai Li
authors.initials: LL
authors.lastname: Yun
mesh_terms: D016207:Cytokines / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008213:Lymphocyte Activation; D010641:Phenotype; D013194:Staining and Labeling / Q000379:methods; D013601:T-Lymphocytes / Q000166:cytology* / Q000502:physiology*
chemical_list: D016207:Cytokines