title: Complete genomic sequence and comparative analysis of the genome segments of sweet potato chlorotic stunt virus in China.
abstract: BACKGROUND
  Sweet potato chlorotic stunt virus (family Closteroviridae, genus Crinivirus) features a large bipartite, single-stranded, positive-sense RNA genome. To date, only three complete genomic sequences of SPCSV can be accessed through GenBank. SPCSV was first detected from China in 2011, only partial genomic sequences have been determined in the country. No report on the complete genomic sequence and genome structure of Chinese SPCSV isolates or the genetic relation between isolates from China and other countries is available.
  
  
  RESULTS
  The complete genomic sequences of five isolates from different areas in China were characterized. This study is the first to report the complete genome sequences of SPCSV from whitefly vectors. Genome structure analysis showed that isolates of WA and EA strains from China have the same coding protein as isolates Can181-9 and m2-47, respectively. Twenty cp genes and four RNA1 partial segments were sequenced and analyzed, and the nucleotide identities of complete genomic, cp, and RNA1 partial sequences were determined. Results indicated high conservation among strains and significant differences between WA and EA strains. Genetic analysis demonstrated that, except for isolates from Guangdong Province, SPCSVs from other areas belong to the WA strain. Genome organization analysis showed that the isolates in this study lack the p22 gene.
  
  
  CONCLUSIONS
  We presented the complete genome sequences of SPCSV in China. Comparison of nucleotide identities and genome structures between these isolates and previously reported isolates showed slight differences. The nucleotide identities of different SPCSV isolates showed high conservation among strains and significant differences between strains. All nine isolates in this study lacked p22 gene. WA strains were more extensively distributed than EA strains in China. These data provide important insights into the molecular variation and genomic structure of SPCSV in China as well as genetic relationships among isolates from China and other countries.
authors.affiliation: Key Laboratory of Crop Pest Control of Henan Province, Key Laboratory of Pest Management in South of North-China for Ministry of Agriculture of PRC, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Qin
authors.affiliation: School of Life Sciences and technology, Nanyang Normal University, Nanyang, Henan, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Crop Pest Control of Henan Province, Key Laboratory of Pest Management in South of North-China for Ministry of Agriculture of PRC, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China.
authors.forename: Zhenchen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Crop Pest Control of Henan Province, Key Laboratory of Pest Management in South of North-China for Ministry of Agriculture of PRC, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Qiao
authors.affiliation: Key Laboratory of Crop Pest Control of Henan Province, Key Laboratory of Pest Management in South of North-China for Ministry of Agriculture of PRC, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China.
authors.forename: Desheng
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Crop Pest Control of Henan Province, Key Laboratory of Pest Management in South of North-China for Ministry of Agriculture of PRC, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Key Laboratory of Crop Pest Control of Henan Province, Key Laboratory of Pest Management in South of North-China for Ministry of Agriculture of PRC, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Crop Pest Control of Henan Province, Key Laboratory of Pest Management in South of North-China for Ministry of Agriculture of PRC, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China.
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Agricultural Economics and Information, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China.
authors.forename: Zhaoling
authors.initials: Z
authors.lastname: Yan
mesh_terms: D001483:Base Sequence; D002681:China; D017124:Conserved Sequence; D029383:Crinivirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016679:Genome, Viral; D027723:Ipomoea batatas / Q000821:virology*; D010802:Phylogeny; D012367:RNA, Viral / Q000032:analysis; D017423:Sequence Analysis, RNA
chemical_list: D012367:RNA, Viral