title: MRI molecular imaging using GLUT1 antibody-Fe3O4 nanoparticles in the hemangioma animal model for differentiating infantile hemangioma from vascular malformation.
abstract: The purpose of this study is to evaluate the efficacy of glucose transporter protein 1 (GLUT1) antibody-conjugated iron oxide nanoparticles (Fe3O4 NPs) as magnetic resonance imaging (MRI) molecular imaging agents for differentiating infantile hemangioma from vascular malformation in the hemangioma animal model. The conjugation of Fe3O4 NPs with anti-GLUT1 antibodies leads to a significantly increased uptake of NPs by human umbilical vein endothelial cells. MRI imaging following the intravenous injection of GLUT1 antibody-Fe3O4 NPs yielded a significantly lower signal intensity than did unconjugated Fe3O4 NPs. Upon histological examination of the GLUT1 antibody-Fe3O4 NPs, Prussian blue-stained NPs were identified in CD31-positive endothelial cells of hemangioma. In contrast, when treated with unconjugated Fe3O4 NPs, Prussian blue-stained NPs were found in macrophages rather than in endothelial cells. GLUT1 antibody conjugation can effectively target the injected Fe3O4 NPs to GLUT1-positive tumor cells in infantile hemangioma.
authors.affiliation: Department of Radiology, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Chul-Ho
authors.initials: CH
authors.lastname: Sohn
authors.affiliation: Center for Nanoparticle Research, Institute for Basic Science, and School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
authors.forename: Seung Pyo
authors.initials: SP
authors.lastname: Park
authors.affiliation: Center for Nanoparticle Research, Institute for Basic Science, and School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
authors.forename: Seung Hong
authors.initials: SH
authors.lastname: Choi
authors.affiliation: Department of Pathology, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Sung-Hye
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Plastic and Reconstructive Surgery, Institute of Human-Environment Interface Biology, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Sukwha
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Plastic and Reconstructive Surgery, Beijing Tongren Hospital, Capital Medical University, People's Republic of China.
authors.forename: Lianji
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Internal Medicine, Keimyung University Dongsan Medical Center, Daegu, Republic of Korea.
authors.forename: Sang-Hyon
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, School of Medicine, Yeungnam University, Daegu, Republic of Korea.
authors.forename: Ji An
authors.initials: JA
authors.lastname: Hur
authors.affiliation: Department of Plastic and Reconstructive Surgery, Keimyung University School of Medicine, Daegu, Republic of Korea.
authors.forename: Jaehoon
authors.initials: J
authors.lastname: Choi
authors.affiliation: Department of Plastic and Reconstructive Surgery, Institute of Human-Environment Interface Biology, Seoul National University College of Medicine, Seoul, Republic of Korea. Electronic address: psthchoi@snu.ac.kr.
authors.forename: Tae Hyun
authors.initials: TH
authors.lastname: Choi
mesh_terms: D000818:Animals; D004396:Coloring Agents / Q000737:chemistry; D003287:Contrast Media / Q000737:chemistry; D003937:Diagnosis, Differential; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry; D005295:Ferrocyanides / Q000737:chemistry; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D006391:Hemangioma / Q000175:diagnosis*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D023421:Models, Animal; D057054:Molecular Imaging / Q000379:methods*; D054079:Vascular Malformations / Q000175:diagnosis*
chemical_list: D004396:Coloring Agents; D003287:Contrast Media; D005290:Ferric Compounds; D005295:Ferrocyanides; D051272:Glucose Transporter Type 1; D058185:Magnetite Nanoparticles; C495677:SLC2A1 protein, human; C000499:ferric oxide; C000170:ferric ferrocyanide
keywords: GLUT1; Infantile hemangioma; Iron oxide nanoparticle; MRI