title: microRNA-183 plays as oncogenes by increasing cell proliferation, migration and invasion via targeting protein phosphatase 2A in renal cancer cells.
abstract: The aim of this study was to investigate the function of miR-183 in renal cancer cells and the mechanisms miR-183 regulates this process. In this study, level of miR-183 in clinical renal cancer specimens was detected by quantitative real-time PCR. miR-183 was up- and down-regulated in two renal cancer cell lines ACHN and A498, respectively, and cell proliferation, Caspase 3/7 activity, colony formation, in vitro migration and invasion were measured; and then the mechanisms of miR-183 regulating was analyzed. We found that miR-183 was up-regulated in renal cancer tissues; inhibition of endogenous miR-183 suppressed in vitro cell proliferation, colony formation, migration, and invasion and stimulated Caspase 3/7 activity; up-regulated miR-183 increased cell growth and metastasis and suppressed Caspase 3/7 activity. We also found that miR-183 directly targeted tumor suppressor, specifically the 3'UTR of three subunits of protein phosphatase 2A (PP2A-Cα, PP2A-Cβ, and PP2A-B56-γ) transcripts, inhibiting their expression and regulated the downstream regulators p21, p27, MMP2/3/7 and TIMP1/2/3/4. These results revealed the oncogenes role of miR-183 in renal cancer cells via direct targeting protein phosphatase 2A.
authors.affiliation: Laboratory of Urology, Guangdong Medical College, Zhanjiang 524001, China. Electronic address: lcuzfy@163.com.
authors.forename: Mingning
authors.initials: M
authors.lastname: Qiu
authors.affiliation: Laboratory of Urology, Guangdong Medical College, Zhanjiang 524001, China. Electronic address: leiliulab@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Laboratory of Urology, Guangdong Medical College, Zhanjiang 524001, China. Electronic address: lieqianchen@163.com.
authors.forename: Lieqian
authors.initials: L
authors.lastname: Chen
authors.affiliation: Laboratory of Urology, Guangdong Medical College, Zhanjiang 524001, China. Electronic address: guobintan@163.com.
authors.forename: Guobin
authors.initials: G
authors.lastname: Tan
authors.affiliation: Laboratory of Urology, Guangdong Medical College, Zhanjiang 524001, China. Electronic address: zijilianglab@163.com.
authors.forename: Ziji
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Laboratory of Urology, Guangdong Medical College, Zhanjiang 524001, China. Electronic address: kangningwanglab@163.com.
authors.forename: Kangning
authors.initials: K
authors.lastname: Wang
authors.affiliation: Laboratory of Urology, Guangdong Medical College, Zhanjiang 524001, China. Electronic address: jianjunliulab@163.com.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Laboratory of Urology, Guangdong Medical College, Zhanjiang 524001, China. Electronic address: hegechen@163.com.
authors.forename: Hege
authors.initials: H
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D017931:DNA Primers; D006801:Humans; D007680:Kidney Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D009857:Oncogenes; D054648:Protein Phosphatase 2 / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; C533449:MIRN183 microRNA, human; D035683:MicroRNAs; D054648:Protein Phosphatase 2
keywords: Protein phosphatase 2A; Renal cancer cells; miR-183