title: Fluorescent sensors reveal subcellular thermal changes.
abstract: In mammals and birds, thermoregulation to conserve body temperature is vital to life. Multiple mechanisms of thermogeneration have been proposed, localized in different subcellular organelles. However, studying thermogenesis directly in intact organelles has been challenging. Visualizing patterns of thermal changes at subcellular resolution would reveal physiologically relevant spatio-temporal information, especially if this could be done in the native cellular configuration of the cell. Here we review and compare the wide variety of intracellular thermosensors currently identified. This review focuses particularly on genetically encoded sensors. It also explores the notable physiological discoveries made using these imaging methods, which are rapidly becoming indispensible to the study of thermal biology.
authors.affiliation: World Premier International Research Initiative-Institute for Integrated Cell-Material Sciences, Kyoto University, Kyoto 606-8501, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Sakaguchi
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan; Department of Technology and Ecology, Hall of Global Environmental Studies, Kyoto University, Kyoto 615-8510, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Kiyonaka
authors.affiliation: World Premier International Research Initiative-Institute for Integrated Cell-Material Sciences, Kyoto University, Kyoto 606-8501, Japan; Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan; Department of Technology and Ecology, Hall of Global Environmental Studies, Kyoto University, Kyoto 615-8510, Japan. Electronic address: mori@sbchem.kyoto-u.ac.jp.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Mori
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000032:analysis*; D006801:Humans; D042541:Intracellular Space / Q000737:chemistry*; D036103:Nanotechnology; D011108:Polymers / Q000737:chemistry; D013696:Temperature
chemical_list: D005456:Fluorescent Dyes; D011108:Polymers