title: 7,8,4'-Trihydroxyisoflavone attenuates DNCB-induced atopic dermatitis-like symptoms in NC/Nga mice.
abstract: Atopic dermatitis (AD) is characterized by chronic highly pruritic and relapsing inflammatory skin lesions. Despite its growing prevalence, therapeutic treatments remain limited. Natural immune modulators from herbal extracts or derivatives may be useful for treating AD symptoms. This study examined the effect of 7,8,4'-trihydroxyisoflavone (7,8,4'-THIF), a metabolite of soy isoflavone daidzin, on AD-like symptoms. Repeated epicutaneous application of 2,4-dinitrochlorobenzene (DNCB) was performed on the ear and dorsal skin of NC/Nga mice to induce AD-like symptoms and skin lesions, and 7,8,4'-THIF (200 and 400 nmol) or tacrolimus (100 µg) was applied topically for 3 weeks to assess their anti-pruritic effects. We found that 7,8,4'-THIF alleviated DNCB-induced AD-like symptoms as quantified by skin lesion, dermatitis score, ear thickness, and scratching behavior. Histopathological analysis demonstrated that 7,8,4'-THIF decreased DNCB-induced eosinophil and mast cell infiltration into skin lesions. We also found that 7,8,4'-THIF significantly alleviated DNCB-induced loss of water through the epidermal layer. In addition to reducing the DNCB-induced increase in serum IgE, 7,8,4'-THIF also lowered skin lesion levels of the chemokine thymus and activation regulated chemokine; Th2 cytokines interleukin (IL)-4, IL-5, and IL-13; and Th1 cytokines IL-12 and interferon-γ. These results suggest that 7,8,4'-THIF might be a potential therapeutic candidate for the treatment of atopic dermatitis.
authors.affiliation: WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
authors.forename: Heejung
authors.initials: H
authors.lastname: Kim
authors.affiliation: WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea; Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Republic of Korea.
authors.forename: Jong Rhan
authors.initials: JR
authors.lastname: Kim
authors.affiliation: WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
authors.forename: Heerim
authors.initials: H
authors.lastname: Kang
authors.affiliation: WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jinhwan
authors.initials: J
authors.lastname: Choi
authors.affiliation: WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
authors.forename: Hee
authors.initials: H
authors.lastname: Yang
authors.affiliation: Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Republic of Korea.
authors.forename: Pomjoo
authors.initials: P
authors.lastname: Lee
authors.affiliation: Center for Food and Bioconvergence, Seoul National University, Seoul, Republic of Korea; Laboratory of Toxicology, Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Kim
authors.affiliation: WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea; Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Republic of Korea; Center for Food and Bioconvergence, Seoul National University, Seoul, Republic of Korea; Research Institute of Bio Food Industry, Institute of Green Bio Science and Technology, Seoul National University, Pyeongchang, Republic of Korea.
authors.forename: Ki Won
authors.initials: KW
authors.lastname: Lee
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D016207:Cytokines / Q000032:analysis / Q000276:immunology; D003876:Dermatitis, Atopic / Q000139:chemically induced* / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D004137:Dinitrochlorobenzene; D005260:Female; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007529:Isoflavones / Q000627:therapeutic use*; D051379:Mice; D012867:Skin / Q000187:drug effects* / Q000276:immunology / Q000473:pathology*
chemical_list: C502165:7,8,4'-trihydroxyisoflavone; D000893:Anti-Inflammatory Agents; D016207:Cytokines; D004137:Dinitrochlorobenzene; D007529:Isoflavones; D007073:Immunoglobulin E