title: [Alternation of retinal complement system in a mouse model of lipopolysaccharide- induced uveitis].
abstract: OBJECTIVE
  To investigate the activation of the complement system in the retina in a mouse model of endotoxin-induced uveitis (EIU).
  
  
  METHODS
  Balb/c mice were randomly divided into control group and lipopolysaccharide (LPS)-induced EIU group. Twenty-four hours after modeling, the expressions of the major complement components of the classical pathway (CP), mannose-binding lectin (MBL) pathway, alternative pathway (AP), and terminal pathway in the retina were determined by quantitative RT-PCR. Western blotting was employed to examine the protein expressions of the key components of the complement system involved in the CP and AP pathways in the retina.
  
  
  RESULTS
  s Normal mouse retina expressed a variety of complement components that were involved mainly in the CP and AP pathways. The expressions of the complement components involved in the CP and AP pathways were up-regulated in the retina of mice with EIU. Although MASP1 and MASP2 were detected in the retina in both the EIU and control groups, their expressions were weak and showed no significant difference between the two groups.
  
  
  CONCLUSIONS
  The AP and CP but not the MBL pathways of the complement system are activated in the retina of mice with EIU, suggesting a role of the activated complement system in the pathological process of EIU.
authors.affiliation: Department of Ophthalmology, First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing 400016, China.E-mail: 1273438489@qq.com.
authors.forename: Xueying
authors.initials: X
authors.lastname: Tao
authors.forename: Shijie
authors.initials: S
authors.lastname: Zheng
authors.forename: Bo
authors.initials: B
authors.lastname: Lei
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003165:Complement System Proteins; D004195:Disease Models, Animal; D008070:Lipopolysaccharides; D051379:Mice; D008807:Mice, Inbred BALB C; D012160:Retina / Q000503:physiopathology*; D014605:Uveitis / Q000139:chemically induced / Q000503:physiopathology*
chemical_list: D008070:Lipopolysaccharides; D003165:Complement System Proteins