title: A monoclonal antibody based enzyme-linked immunosorbent assay for detection of phenylethanolamine A in tissue of swine.
abstract: Phenylethanolamine A (PEA) is a phenethanolamine member of the family of β-adrenergic agonists compounds illegally used as feed additives for growth promotion. In this study, PEA was covalently linked to carrier protein cationized bovine serum albumin (cBSA) and egg albumin (OVA). A monoclonal antibody specific for PEA was produced and characteristics of monoclonal antibody (McAb) were studied. Based on the McAb, an enzyme-linked immunosorbent assay (ELISA) for detection of PEA was developed, which showed an IC50 value of 6.25 ng mL(-1) and a detection limit of 0.19 ng mL(-1). The average recovery of PEA from spiked samples was 103.4%, which demonstrated that the matrices of meat where PEA may be found do not interfere with the assay.
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education/Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Xi An Da Lu 5333, Changchun 130062, PR China.
authors.forename: Yansong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education/Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Xi An Da Lu 5333, Changchun 130062, PR China.
authors.forename: Shiying
authors.initials: S
authors.lastname: Lu
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education/Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Xi An Da Lu 5333, Changchun 130062, PR China.
authors.forename: Zengshan
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Jilin Bussiness and Technology College, Jilin University, Changchun 130062, PR China.
authors.forename: Lihui
authors.initials: L
authors.lastname: Sun
authors.affiliation: Jilin Bussiness and Technology College, Jilin University, Changchun 130062, PR China.
authors.forename: Junfei
authors.initials: J
authors.lastname: Guo
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education/Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Xi An Da Lu 5333, Changchun 130062, PR China.
authors.forename: Pan
authors.initials: P
authors.lastname: Hu
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education/Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Xi An Da Lu 5333, Changchun 130062, PR China.
authors.forename: Junhui
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education/Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Xi An Da Lu 5333, Changchun 130062, PR China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education/Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Xi An Da Lu 5333, Changchun 130062, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education/Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Xi An Da Lu 5333, Changchun 130062, PR China.
authors.forename: Honglin
authors.initials: H
authors.lastname: Ren
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education/Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Xi An Da Lu 5333, Changchun 130062, PR China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Meng
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education/Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Xi An Da Lu 5333, Changchun 130062, PR China. Electronic address: zhouyu69@sina.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004983:Ethanolamines / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D004983:Ethanolamines
keywords: Assay; Monoclonal antibody (McAb); Phenylethanolamine A (PEA)