title: Immobilization of trypsin on plasma prepared Ag/PPAni nanocomposite film for efficient digestion of protein.
abstract: This work demonstrates the efficacy of a support matrix prepared by plasma process for trypsin immobilization without any surface activator. Plasma polymerization cum sputtering process is used to prepare the nanocomposite support matrix. Plasma sputtered silver nanoparticles (AgNPs) are uniformly embedded into plasma polymerized aniline (PPAni) film. Various characterization tools are employed to study the surface morphology, microstructure and chemical composition of the support matrices. Trypsin is immobilized onto the support matrix via the formation of covalent bond between them. Plasma generated free radicals on composite films activate the support matrix and make it efficient for increasing the tertiary enzyme stability via multipoint covalent attachment. Trypsin immobilized onto Ag/PPAni matrix has more hydrolyzing capacity of bovine serum albumin (BSA) than free trypsin as well as trypsin immobilized onto PPAni films.
authors.affiliation: Physical Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati 781035, India.
authors.forename: Dolly
authors.initials: D
authors.lastname: Gogoi
authors.affiliation: Physical Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati 781035, India.
authors.forename: Tapan
authors.initials: T
authors.lastname: Barman
authors.affiliation: Guwahati Biotech Park, Technology Complex, IIT-Guwahati, Guwahati 781039, India.
authors.forename: Bula
authors.initials: B
authors.lastname: Choudhury
authors.affiliation: Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati 781035, India.
authors.forename: Mojibur
authors.initials: M
authors.lastname: Khan
authors.affiliation: Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati 781035, India.
authors.forename: Yogesh
authors.initials: Y
authors.lastname: Chaudhari
authors.affiliation: Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati 781035, India.
authors.forename: Madhusmita
authors.initials: M
authors.lastname: Dehingia
authors.affiliation: Physical Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati 781035, India. Electronic address: arpal@iasst.gov.in.
authors.forename: Arup Ratan
authors.initials: AR
authors.lastname: Pal
authors.affiliation: Physical Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati 781035, India.
authors.forename: Heremba
authors.initials: H
authors.lastname: Bailung
authors.affiliation: Physical Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati 781035, India.
authors.forename: Joyanti
authors.initials: J
authors.lastname: Chutia
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006868:Hydrolysis; D053768:Metal Nanoparticles; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites; D011506:Proteins / Q000378:metabolism*; D012834:Silver / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014357:Trypsin / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D011506:Proteins; D012834:Silver; D014357:Trypsin
keywords: Hydrolyzing capacity; Nanocomposite support matrix; Plasma polymerization; Sputtering; Trypsin immobilization