title: [RNAi-mediated silencing of CD147 inhibits the proliferation and infiltrations of leukemic cells SHI-1].
abstract: OBJECTIVE
  To investigate the role of CD147 gene on the proliferation and infiltration of a human monocytic leukemic cell line SHI-1.
  
  
  METHODS
  The expression of CD147 in SHI-1 cells was knockdowned by the lentiviral vector. The expressions of CD147, MMP-2 and MMP-9 were detected by semiquantitative RT-PCR. The protein of CD147 was detected by Western blotting. The capabilities of proliferation and infiltration of SHI-1 cell were examined by MTT and trans- matrigel invasion assay co-cultured with leukemia BMSC in vitro. SHI-1 cells were inoculated subcutaneously or via tail vein into nude mice to investigate its growth and infiltrative ability in vivo.
  
  
  RESULTS
  The mRNA and protein of CD147 in SHI-1/CD147i cells decreased by 85% and 91%, respectively after the SHI-1 cells were infected by the lentivirus containing the CD147 siRNA. The proliferation capability of SHI-1/CD147i cells significantly decreased than those of SHI-1 and SHI-1/NC cells. The mRNA expressions of MMP-2, MMP-9 in SHI-1/CD147i cells were significantly lower than those in SHI-1/NC and SHI-1 cells. The SHI-1/CD147i cells showed significantly lower invasion rate than SHI-1 cells and SHI-1/NC cells when co-cultured with BMSCs. The neoplasms formed by SHI-1/CD147i cells in the subcutaneous of mice were significantly smaller than of the neoplasms formed by SHI-1 and SHI-1/NC cells. In nude mice inoculated via caudal vein with SHI/CD147i cells, mice demonstrated longer survival and moderate infiltration characteristic than those inoculated with SHI and SHI-1/NC cells.
  
  
  CONCLUSIONS
  CD147 might play important roles in the proliferation and infiltration of leukemia cells. CD147 should be a potential target for the treatment of acute leukemia.
authors.affiliation: Department of Hematology, the Second Hospital Affiliated to Nanchang University, Nanchang 330006, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Tu
authors.affiliation: Department of Hematology, the Second Hospital Affiliated to Nanchang University, Nanchang 330006, China.
authors.forename: Zhenjiang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Hematology, the Second Hospital Affiliated to Nanchang University, Nanchang 330006, China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Tang
authors.affiliation: Department of Hematology, the Second Hospital Affiliated to Nanchang University, Nanchang 330006, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Fei
authors.affiliation: Department of Hematology, the Second Hospital Affiliated to Nanchang University, Nanchang 330006, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Hematology, the Second Hospital Affiliated to Nanchang University, Nanchang 330006, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Hematology, the Second Hospital Affiliated to Nanchang University, Nanchang 330006, China.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: He
mesh_terms: D000818:Animals; D051926:Basigin / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D055785:Gene Knockdown Techniques; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D051926:Basigin; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9