title: Transcriptional regulation of dentin matrix protein 1 (DMP1) in odontoblasts and osteoblasts.
abstract: Dentin matrix protein 1 (DMP1) is a noncollagenous protein important for the mineralization of bones and teeth. Examination of the transcription factor binding sites within the 6.24 kb upstream sequence of rat DMP1 promoter by Matinspector software revealed that TCF11 had the highest number (six) of binding sites with 100% matrix similarity. Four of these sites are conserved in the mouse DMP1 promoter. TCF11 is a member of the Cap-n-Collar (cnc) family of basic leucine zipper transcription factors. Results from this study showed that TCF11 can bind specifically to the DMP1 promoter and activate its transcription in odontoblasts and osteoblasts. This could be attributed to both direct and indirect effects of TCF11. Electrophoretic mobility shift (EMSA) assay showed differential interaction between TCF11 and its binding sites on the DMP1 promoter. 21 bp oligos spanning the TCF11 matrix were used as probes in EMSA, and the results showed that the binding was specific to the sequence of the TCF11 matrix as well as the flanking sequences and this is typical of a heterodimer binding site. Results also showed changes in the binding pattern when cells were differentiated in osteogenic medium for 2 d. Thus, TCF11 may play an important role in the transcriptional regulation of DMP1 gene.
authors.affiliation: Brodie Tooth Development Genetics & Regenerative Medicine Research Laboratory, Department of Oral Biology, University of Illinois , Chicago, IL , USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Jacob
authors.forename: Youbin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Anne
authors.initials: A
authors.lastname: George
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D003804:Dentin / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D009804:Odontoblasts / Q000378:metabolism*; D010006:Osteoblasts / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D051381:Rats; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: C081635:Dmp1 protein, rat; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D014157:Transcription Factors
keywords: DMP1; MC3T3 cells; T4-4 cells; TCF11; bZip proteins; mineralization; odontoblasts; osteoblasts