title: Metabotropic glutamate receptor 1 splice variants mGluR1a and mGluR1b combine in mGluR1a/b dimers in vivo.
abstract: The assembly of two covalently linked monomers into dimeric complexes is a prerequisite for metabotropic glutamate receptor 1 (mGluR1) function. The former concept of a strictly homodimeric subunit contribution in metabotropic glutamate receptor complexes has recently been brought into question. Alternative splicing of the GRM1 gene results in expression of variants that vary within their intracellular C-termini. Here we bring evidence that the short mGluR1b variant is found preferentially in a complex with the long mGluR1a variant in the rodent brain. The mGluR1a and mGluR1b variants distribution overlaps in Purkinje cells and the two variants colocalize in their spines. However mGluR1a and mGluR1b show distinct sub-cellular localization when expressed alone in neurons. We discovered that trafficking of mGluR1b to distal dendrites is reliant on its association with mGluR1a and that the long C-terminus of mGluR1a within the mGluR1a/b dimer is necessary for trafficking of the complex.
authors.affiliation: Institute of Molecular Genetics, Academy of Science of the Czech Republic, Videnska 1083, 14220 Prague 4, Czech Republic.
authors.forename: Šárka
authors.initials: Š
authors.lastname: Techlovská
authors.affiliation: Institute of Molecular Genetics, Academy of Science of the Czech Republic, Videnska 1083, 14220 Prague 4, Czech Republic.
authors.forename: Jayne Nicole
authors.initials: JN
authors.lastname: Chambers
authors.affiliation: Institute of Molecular Genetics, Academy of Science of the Czech Republic, Videnska 1083, 14220 Prague 4, Czech Republic.
authors.forename: Michaela
authors.initials: M
authors.lastname: Dvořáková
authors.affiliation: National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: Ronald S
authors.initials: RS
authors.lastname: Petralia
authors.affiliation: National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: Ya-Xian
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Institute of Molecular Genetics, Academy of Science of the Czech Republic, Videnska 1083, 14220 Prague 4, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Hájková
authors.affiliation: Institute of Molecular Genetics, Academy of Science of the Czech Republic, Videnska 1083, 14220 Prague 4, Czech Republic.
authors.forename: Alice
authors.initials: A
authors.lastname: Nová
authors.affiliation: Institute of Molecular Genetics, Academy of Science of the Czech Republic, Videnska 1083, 14220 Prague 4, Czech Republic.
authors.forename: Daniela
authors.initials: D
authors.lastname: Franková
authors.affiliation: CNRS UMR5203, Institut de génomique fonctionnelle, Montpellier, France.|INSERM U661, Montpellier, France.|Université Montpellier 1 & 2, Montpellier F-34000, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Prezeau
authors.affiliation: Institute of Molecular Genetics, Academy of Science of the Czech Republic, Videnska 1083, 14220 Prague 4, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Blahos
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D001921:Brain / Q000378:metabolism*; D002478:Cells, Cultured; D049229:Dendritic Spines / Q000378:metabolism; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D057809:HEK293 Cells; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009474:Neurons / Q000378:metabolism*; D020033:Protein Isoforms; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D018094:Receptors, Metabotropic Glutamate / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D020033:Protein Isoforms; D018094:Receptors, Metabotropic Glutamate; C104077:metabotropic glutamate receptor type 1
keywords: Alternative splicing; G-protein coupled receptors (GPCR); Metabotropic glutamate receptors; Protein–protein interactions; Trafficking