title: X-ray nanoprobes and diffraction-limited storage rings: opportunities and challenges of fluorescence tomography of biological specimens.
abstract: X-ray nanoprobes require coherent illumination to achieve optic-limited resolution, and so will benefit directly from diffraction-limited storage rings. Here, the example of high-resolution X-ray fluorescence tomography is focused on as one of the most voracious demanders of coherent photons, since the detected signal is only a small fraction of the incident flux. Alternative schemes are considered for beam delivery, sample scanning and detectors. One must consider as well the steps before and after the X-ray experiment: sample preparation and examination conditions, and analysis complexity due to minimum dose requirements and self-absorption. By understanding the requirements and opportunities for nanoscale fluorescence tomography, one gains insight into the R&D challenges in optics and instrumentation needed to fully exploit the source advances that diffraction-limited storage rings offer.
authors.affiliation: Australian Synchrotron, 800 Blackburn Road, Clayton, Victoria 3168, Australia.
authors.forename: Martin D
authors.initials: MD
authors.lastname: de Jonge
authors.affiliation: CSIRO Earth Science and Research Engineering, Clayton, Victoria 3168, Australia.
authors.forename: Christopher G
authors.initials: CG
authors.lastname: Ryan
authors.affiliation: Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439, USA.
authors.forename: Chris J
authors.initials: CJ
authors.lastname: Jacobsen
mesh_terms: D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods; D008856:Microscopy, Fluorescence; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D053758:Nanoparticles; D055095:Optics and Photonics; D011829:Radiation Dosage; D017356:Synchrotrons; D014961:X-Ray Diffraction / Q000295:instrumentation / Q000379:methods*; D055114:X-Ray Microtomography / Q000295:instrumentation / Q000379:methods*
keywords: X-ray fluorescence microscopy; diffraction-limited storage rings; fluorescence tomography; nanotomography; scanning X-ray microscopy