title: Personalized metabolomics for predicting glucose tolerance changes in sedentary women after high-intensity interval training.
abstract: High-intensity interval training (HIIT) offers a practical approach for enhancing cardiorespiratory fitness, however its role in improving glucose regulation among sedentary yet normoglycemic women remains unclear. Herein, multi-segment injection capillary electrophoresis-mass spectrometry is used as a high-throughput platform in metabolomics to assess dynamic responses of overweight/obese women (BMI > 25, n = 11) to standardized oral glucose tolerance tests (OGTTs) performed before and after a 6-week HIIT intervention. Various statistical methods were used to classify plasma metabolic signatures associated with post-prandial glucose and/or training status when using a repeated measures/cross-over study design. Branched-chain/aromatic amino acids and other intermediates of urea cycle and carnitine metabolism decreased over time in plasma after oral glucose loading. Adaptive exercise-induced changes to plasma thiol redox and orthinine status were measured for trained subjects while at rest in a fasting state. A multi-linear regression model was developed to predict changes in glucose tolerance based on a panel of plasma metabolites measured for naïve subjects in their untrained state. Since treatment outcomes to physical activity are variable between-subjects, prognostic markers offer a novel approach to screen for potential negative responders while designing lifestyle modifications that maximize the salutary benefits of exercise for diabetes prevention on an individual level.
authors.affiliation: Department of Chemistry and Chemical Biology, McMaster University, Hamilton, Canada.
authors.forename: Naomi L
authors.initials: NL
authors.lastname: Kuehnbaum
authors.affiliation: Department of Kinesiology, McMaster University, Hamilton, Canada.
authors.forename: Jenna B
authors.initials: JB
authors.lastname: Gillen
authors.affiliation: Department of Kinesiology, McMaster University, Hamilton, Canada.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Gibala
authors.affiliation: Department of Kinesiology, McMaster University, Hamilton, Canada.
authors.forename: Philip
authors.initials: P
authors.lastname: Britz-McKibbin
mesh_terms: D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose; D005081:Exercise Therapy; D005260:Female; D018149:Glucose Intolerance; D006801:Humans; D055442:Metabolome; D009765:Obesity / Q000097:blood* / Q000628:therapy; D064797:Physical Conditioning, Human; D010807:Physical Endurance; D057285:Precision Medicine; D057185:Sedentary Behavior
chemical_list: D015415:Biomarkers; D001786:Blood Glucose