title: Affinity purification of binding miRNAs for messenger RNA fused with a common tag.
abstract: Prediction of microRNA-mRNA interaction typically relies on bioinformatic methods, but these methods only suggest the possibility of microRNA binding and may miss important interactions as well as falsely predict others. A major obstacle to the miRNA research has been the lack of experimental procedures for the identification of miRNA-mRNA interactions. Recently, a few studies have attempted to explore experimental methods to isolate and identify miRNA targets or miRNAs targeting a single gene. Here, we developed an more convenient experimental approach for the isolation and identification of miRNAs targeting a single gene by applying short biotinylated DNA anti-sense oligonucleotides mix to enhanced green fluorescent protein (EGFP) mRNA which was fused to target gene mRNA. This method does not require a design of different anti-sense oligonucleotides to any mRNA. This is a simple and an efficient method to potentially identify miRNAs targeting specific gene mRNA combined with chip screen.
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Science, Hunan Normal University, Changsha 410081, China. weike0831@gmail.com.
authors.forename: Ke
authors.initials: K
authors.lastname: Wei
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Science, Hunan Normal University, Changsha 410081, China. yanfengwujinjin@gmail.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Yan
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Science, Hunan Normal University, Changsha 410081, China. huix1125@gmail.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Science, Hunan Normal University, Changsha 410081, China. yangxiaoxu@hunnu.edu.cn.
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Yang
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Science, Hunan Normal University, Changsha 410081, China. guiexie@gmail.com.
authors.forename: Guie
authors.initials: G
authors.lastname: Xie
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Science, Hunan Normal University, Changsha 410081, China. xiaoy5488@gmail.com.
authors.forename: Ye
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Science, Hunan Normal University, Changsha 410081, China. paopaoguqiqiu@gmail.com.
authors.forename: Tingting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Science, Hunan Normal University, Changsha 410081, China. xiaoy5488@gmail.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xun
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Science, Hunan Normal University, Changsha 410081, China. yimijiayou@gmail.com.
authors.forename: Zhaoqin
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Science, Hunan Normal University, Changsha 410081, China. hanmei@hunnu.edu.cn.
authors.forename: Mei
authors.initials: M
authors.lastname: Han
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Science, Hunan Normal University, Changsha 410081, China. zhangjian@hunnu.edu.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Science, Hunan Normal University, Changsha 410081, China. xshlin@hunnu.edu.cn.
authors.forename: Shuanglin
authors.initials: S
authors.lastname: Xiang
mesh_terms: D002846:Chromatography, Affinity; D049452:Green Fluorescent Proteins / Q000737:chemistry; D035683:MicroRNAs / Q000737:chemistry*; D016376:Oligonucleotides, Antisense / Q000737:chemistry; D012333:RNA, Messenger / Q000737:chemistry*
chemical_list: D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins