title: Decellularization of human dermis using non-denaturing anionic detergent and endonuclease: a review.
abstract: Decellularized human dermis has been used for a number of clinical applications including wound healing, soft tissue reconstruction, and sports medicine procedures. A variety of methods exist to prepare this useful class of biomaterial. Here, we describe a decellularization technology (MatrACELL(®)) utilizing a non-denaturing anionic detergent, N-Lauroyl sarcosinate, and endonuclease, which was developed to remove potentially immunogenic material while retaining biomechanical properties. Effective decellularization was demonstrated by a residual DNA content of ≤4 ng/mg of wet weight which represented >97 % DNA removal compared to unprocessed dermis. Two millimeter thick MatrACELL processed human acellular dermal matrix (MH-ADM) exhibited average ultimate tensile load to failure of 635.4 ± 199.9 N and average suture retention strength of 134.9 ± 55.1 N. Using an in vivo mouse skin excisional model, MH-ADM was shown to be biocompatible and capable of supporting cellular and vascular in-growth. Finally, clinical studies of MH-ADM in variety of applications suggest it can be an appropriate scaffold for wound healing, soft tissue reconstruction, and soft tissue augmentation.
authors.affiliation: Institute of Regenerative Medicine, LifeNet Health, 1864 Concert Drive, Virginia Beach, VA, 23453, USA, mark_moore@lifenethealth.org.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Moore
authors.forename: Brian
authors.initials: B
authors.lastname: Samsell
authors.forename: Glenna
authors.initials: G
authors.lastname: Wallis
authors.forename: Sherry
authors.initials: S
authors.lastname: Triplett
authors.forename: Silvia
authors.initials: S
authors.lastname: Chen
authors.forename: Alyce Linthurst
authors.initials: AL
authors.lastname: Jones
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Qin
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D020405:Dermis / Q000166:cytology*; D003902:Detergents / Q000494:pharmacology; D004720:Endonucleases / Q000378:metabolism*; D006801:Humans; D016038:Skin Transplantation / Q000379:methods; D014945:Wound Healing / Q000502:physiology*
chemical_list: D001672:Biocompatible Materials; D003902:Detergents; D004720:Endonucleases