title: Up-regulated expression of PD-1 and its ligands during acute Classical Swine Fever virus infection in swine.
abstract: In order to investigate the relationship between the PD-1 pathway and impairment of immune responses with the CSFV infection, the mRNA expression of PD-1 and its ligands were evaluated by quantitative polymerase chain reaction (qPCR) during artificial CSFV infection. Simultaneously, expression of IL-2 and IL-10 mRNA were detected. The T cell proliferation and CSFV load in plasma were also measured. Results showed that the expression of PD-1 and its ligands mRNA were significantly increased (p < 0.01) in PBMC from 3 to 7 days post infection (dpi). Meanwhile the level of IL-10 was up-regulated (p < 0.01). The IL-2 mRNA was not obviously changed but it is significantly increased from 14 dpi. The T cell proliferation was notably decreased at 7 dpi. The CSFV load was also increased in plasma. Overall, our results suggest that the expression of PD-1 and its ligands were up-regulated and probably correlated with immune inhibition during acute CSFV infection.
authors.affiliation: Department of Life Sciense and Technology, Xinxiang University, Xinxiang, Henan 453003 China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yue
authors.affiliation: Department of Life Sciense and Technology, Xinxiang University, Xinxiang, Henan 453003 China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Zhu
authors.affiliation: Department of Life Sciense and Technology, Xinxiang University, Xinxiang, Henan 453003 China.
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Zhang
authors.affiliation: Department of Life Sciense and Technology, Xinxiang University, Xinxiang, Henan 453003 China.
authors.forename: Guo-Peng
authors.initials: GP
authors.lastname: Sun
authors.affiliation: Department of Life Sciense and Technology, Xinxiang University, Xinxiang, Henan 453003 China; College of Veterinary and Animal Sciences, Henan Istitute of Science and Technology, Xinxiang, Henan 453003, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Life Sciense and Technology, Xinxiang University, Xinxiang, Henan 453003 China.
authors.forename: Dong-Guang
authors.initials: DG
authors.lastname: Guo
authors.affiliation: Department of Life Sciense and Technology, Xinxiang University, Xinxiang, Henan 453003 China; College of Veterinary and Animal Sciences, Henan Istitute of Science and Technology, Xinxiang, Henan 453003, China.
authors.forename: Ai-Guo
authors.initials: AG
authors.lastname: Wang
authors.affiliation: Department of Life Sciense and Technology, Xinxiang University, Xinxiang, Henan 453003 China; College of Veterinary and Animal Sciences, Henan Istitute of Science and Technology, Xinxiang, Henan 453003, China.
authors.forename: Bo-Wen
authors.initials: BW
authors.lastname: Li
authors.affiliation: College of Veterinary and Animal Sciences, Henan Istitute of Science and Technology, Xinxiang, Henan 453003, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yin
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan 611130, China.
authors.forename: An-Chun
authors.initials: AC
authors.lastname: Cheng
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan 611130, China. Electronic address: mshwang@163.com.
authors.forename: Ming-Shu
authors.initials: MS
authors.lastname: Wang
authors.affiliation: Department of Life Sciense and Technology, Xinxiang University, Xinxiang, Henan 453003 China; College of Veterinary and Animal Sciences, Henan Istitute of Science and Technology, Xinxiang, Henan 453003, China. Electronic address: wangxuannian@163.com.
authors.forename: Xuan-Nian
authors.initials: XN
authors.lastname: Wang
mesh_terms: D000818:Animals; D060890:B7-H1 Antigen / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D006691:Classical Swine Fever / Q000378:metabolism* / Q000473:pathology; D006692:Classical Swine Fever Virus; D005260:Female; D016753:Interleukin-10 / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D008297:Male; D061027:Programmed Cell Death 1 Ligand 2 Protein / Q000235:genetics / Q000378:metabolism*; D061026:Programmed Cell Death 1 Receptor / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D013552:Swine; D013601:T-Lymphocytes / Q000473:pathology; D015854:Up-Regulation / Q000235:genetics; D019562:Viral Load
chemical_list: D060890:B7-H1 Antigen; D007376:Interleukin-2; D061027:Programmed Cell Death 1 Ligand 2 Protein; D061026:Programmed Cell Death 1 Receptor; D012333:RNA, Messenger; D016753:Interleukin-10
keywords: Classical swine fever virus; Ligand; Peripheral blood mononucleur cells; Programmed death-1; Swine