title: Aberrant proteomic expression of NSRP70 and its clinical implications and connection to the transcriptional level in adult acute leukemia.
abstract: We investigated three splicing factor proteins (SFPs; NSRP70, SRSF1, and HNRNPA1) in 187 adults with and without acute leukemia (AL). We showed that NSRP70 is a novel lymphoblastic AL (ALL) surrogate marker, which presented excellent diagnostic accuracy (92%) and disappeared during remission. Its highest molecular weight form, but not total amount, was associated with adverse genetic abnormalities in myeloid AL (AML). Furthermore, we identified that these SFPs were more prevalent in ALL than in AML; were not correlated with their mRNA levels; and their formations in AL may occur without coding mutations and relate to post-translational modifications.
authors.affiliation: Department of Laboratory Medicine, School of Medicine, Wonkwang University, Iksan, Republic of Korea; Center for Metabolic Function Regulation, Institute of Wonkwang Medical Science and Institute of Wonkwang Clinical Medicine, Wonkwang University, Iksan, Republic of Korea.
authors.forename: Dan-Bee
authors.initials: DB
authors.lastname: Choi
authors.affiliation: Department of Internal Medicine, School of Medicine, Wonkwang University, Iksan, Republic of Korea.
authors.forename: Moo-Rim
authors.initials: MR
authors.lastname: Park
authors.affiliation: Department of Internal Medicine, School of Medicine, Wonkwang University, Iksan, Republic of Korea.
authors.forename: Hak-Ryul
authors.initials: HR
authors.lastname: Kim
authors.affiliation: School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea.
authors.forename: Chang-Duk
authors.initials: CD
authors.lastname: Jun
authors.affiliation: Department of Internal Medicine and Genome Research Center for Hematopoietic Diseases, Chonnam National University Hwasun Hospital, Hwasun-gun, Republic of Korea.
authors.forename: Hyeoung-Joon
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, School of Medicine, Wonkwang University, Iksan, Republic of Korea.
authors.forename: Hyeok
authors.initials: H
authors.lastname: Shim
authors.affiliation: School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea.
authors.forename: Young-Dae
authors.initials: YD
authors.lastname: Kim
authors.affiliation: Department of Pathology and Genome Research Center for Hematopoietic Diseases, Chonnam National University Hwasun Hospital, Hwasun-gun, Republic of Korea.
authors.forename: Chan
authors.initials: C
authors.lastname: Choi
authors.affiliation: Department of Pathology, School of Medicine, Wonkwang University, Iksan, Republic of Korea.
authors.forename: Keum-Ha
authors.initials: KH
authors.lastname: Choi
authors.affiliation: Department of Pathology, School of Medicine, Wonkwang University, Iksan, Republic of Korea.
authors.forename: Ki Jung
authors.initials: KJ
authors.lastname: Yun
authors.affiliation: Department of Pathology, School of Medicine, Wonkwang University, Iksan, Republic of Korea.
authors.forename: Soo-Cheon
authors.initials: SC
authors.lastname: Chae
authors.affiliation: Center for Metabolic Function Regulation, Institute of Wonkwang Medical Science and Institute of Wonkwang Clinical Medicine, Wonkwang University, Iksan, Republic of Korea; Department of Microbiology, School of Medicine, Wonkwang University, Iksan, Republic of Korea.
authors.forename: Raekil
authors.initials: R
authors.lastname: Park
authors.affiliation: Center for Metabolic Function Regulation, Institute of Wonkwang Medical Science and Institute of Wonkwang Clinical Medicine, Wonkwang University, Iksan, Republic of Korea; Department of Microbiology, School of Medicine, Wonkwang University, Iksan, Republic of Korea.
authors.forename: Seong-Kyu
authors.initials: SK
authors.lastname: Choe
authors.affiliation: Department of Laboratory Medicine, School of Medicine, Wonkwang University, Iksan, Republic of Korea.
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, School of Medicine, Wonkwang University, Iksan, Republic of Korea; Center for Metabolic Function Regulation, Institute of Wonkwang Medical Science and Institute of Wonkwang Clinical Medicine, Wonkwang University, Iksan, Republic of Korea. Electronic address: emailds@hanmail.net.
authors.forename: Do-Sim
authors.initials: DS
authors.lastname: Park
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017398:Alternative Splicing; D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D014158:Transcription, Genetic; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C560091:NSRP1 protein, human; D009687:Nuclear Proteins
keywords: Acute leukemia; HNRNPA1; NSRP70; SRSF1; Splicing factor protein