title: Isolation and characterization of novel Glu-St1 alleles from Pseudoroegneria spicata and Pd. strigosa.
abstract: Pseudoroegneria is a small genus of the Triticeae tribe; its St genome is present in over half of allopolyploid Triticeae species. The high molecular weight (HMW) subunits of glutenin (GS) encoded by the St genome are not well described. In this paper, we report the characterization of fourteen alleles of HMW-GS genes from the two species Pd. spicata and Pd. strigosa. Analysis shows that all fourteen sequences possess a typical primary structure shared by other known HMW-GS, but with some unique modifications. All fourteen Glu-St1 alleles are significantly smaller than normal Glu-1 genes due to fewer repeat motifs in a repetitive region with no indication of large deletion in other conserved regions. Thus, the small size is a common feature of HMW-GS encoded by Glu-St1 loci of Pseudoroegneria species. Sequence analysis indicated that all fourteen Glu-St1 alleles were intermediate type between x- and y-type, which represent an intermediate stage in the evolutionary divergence of x- and y-type subunits.
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Shu-Bin
authors.initials: SB
authors.lastname: Wang
authors.forename: Hua-Nan
authors.initials: HN
authors.lastname: Han
authors.forename: Yu
authors.initials: Y
authors.lastname: Liang
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.forename: Guang-Min
authors.initials: GM
authors.lastname: Xia
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Liu
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005983:Glutens / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D006109:Poaceae / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D005983:Glutens; C037302:glutenin