title: A mouse model of liver injury to evaluate paracrine and endocrine effects of bone marrow mesenchymal stem cells.
abstract: Liver fibrosis is the result of chronic liver disease, which is caused by sustaining multiple damage or injury to the liver. While the liver continues to receive injuries, it suffers from the wound healing process and this eventually leads to the derangement of the liver architecture. Recently, bone marrow-derived mesenchymal stem cells (BM-MSCs) have been suggested to have therapeutic effects in treating liver fibrosis. Here, we describe the isolation, purification, culture, and transplantation of BM-MSCs in the liver fibrosis mouse model, and the assessment of paracrine and endocrine (including androgens and/or estrogens) effects of BM-MSCs in the in vitro cell culture system.
authors.affiliation: George Whipple Lab for Cancer Research, Departments of Pathology, Urology, Radiation Oncology; The Wilmot Cancer Center, University of Rochester Medical Center, Rochester, NY, 14642, USA.
authors.forename: Chiung-Kuei
authors.initials: CK
authors.lastname: Huang
authors.forename: Soo Ok
authors.initials: SO
authors.lastname: Lee
authors.forename: Jie
authors.initials: J
authors.lastname: Luo
authors.forename: RongHao
authors.initials: R
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Dang
authors.forename: Chawnshang
authors.initials: C
authors.lastname: Chang
mesh_terms: D000818:Animals; D002450:Cell Communication; D018929:Cell Culture Techniques; D064987:Cell- and Tissue-Based Therapy; D004195:Disease Models, Animal; D055166:Hepatic Stellate Cells / Q000378:metabolism; D008103:Liver Cirrhosis / Q000378:metabolism / Q000628:therapy; D008107:Liver Diseases / Q000378:metabolism* / Q000628:therapy*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice