title: Knockdown of NOB1 expression inhibits the malignant transformation of human prostate cancer cells.
abstract: Nin one binding-1 protein (NOB1) is a kind of zinc protein involved in ribosome biogenesis and controlled proteolysis. To explore the function of NOB1 in human prostate malignancy, we analyzed the expression of NOB1 in prostate cancer and found that NOB1 was elevated in prostate cancer tissues compared to the adjacent normal tissues. Knockdown of NOB1 by lentivirus-shRNA inhibited the proliferation and colony-formation ability of PC-3 and DU145 prostate cancer cells. Cell cycle analysis showed that silencing of NOB1 caused G0/G1 phase arrest and a slight decrease in S phase (P < 0.05). Furthermore, knockdown of NOB1 significantly suppressed the mobility of PC-3 and DU145 prostate cancer cells (P < 0.05). Collectively, these findings suggested that NOB1 might be involved in tumorigenecity of prostate cancer, and could be a potential molecular target for prostate cancer gene therapy.
authors.affiliation: Department of Urinary Surgery of Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhang
authors.forename: Dongxu
authors.initials: D
authors.lastname: Zhang
authors.forename: Fajun
authors.initials: F
authors.lastname: Qu
authors.forename: Yi
authors.initials: Y
authors.lastname: Hong
authors.forename: Jianwei
authors.initials: J
authors.lastname: Cao
authors.forename: Xiuwu
authors.initials: X
authors.lastname: Pan
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Huang
authors.forename: Hai
authors.initials: H
authors.lastname: Huang
authors.forename: Lei
authors.initials: L
authors.lastname: Yin
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.forename: Jizhong
authors.initials: J
authors.lastname: Ren
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Wang
authors.forename: Danfeng
authors.initials: D
authors.lastname: Xu
authors.forename: Xingang
authors.initials: X
authors.lastname: Cui
mesh_terms: D000728:Androgens / Q000378:metabolism; D000818:Animals; D059447:Cell Cycle Checkpoints / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008297:Male; D008819:Mice, Nude; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: D000728:Androgens; C506664:NOB1 protein, human; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins