title: The inter-individual variability outperforms the intra-individual variability of differentially expressed proteins prior and post irradiation in lymphoblastoid cell lines.
abstract: BACKGROUND
  Radio-sensitivity in normal tissue is characterized by heterogeneity throughout the population and the absence of pre-diagnostic biomarkers.
  
  
  OBJECTIVE
  We conducted a proteomic approach to search for radiation characteristic protein regulation.
  
  
  METHODS
  Cell lines were 10 Gy irradiated and analysed by 2D-DIGE after 24 h. RESULTS were analysed intra- and inter-individually. The principal component analysis and hierarchical clustering was applied to all datasets.
  
  
  RESULTS
  Differences in intra-individual spot abundance prior and post irradiation exactly show the separation of sample classes in two groups: sham-irradiated and irradiated. The inter-individual datasets clustered according to the cell line. The intra-individual differences on protein level after gamma-irradiation are very low, compared with the inter-individual differences among cell lines derived from the same tissue.
  
  
  CONCLUSIONS
  The application of 2-D DIGE may offer a realistic chance for a better molecular characterization of radio-sensitivity and for the discovery of candidate biomarkers.
authors.affiliation: Federal Office for Radiation Protection, Department SG Radiation Protection and Health , Neuherberg , Germany .
authors.forename: A
authors.initials: A
authors.lastname: Gürtler
authors.forename: M
authors.initials: M
authors.lastname: Hauptmann
authors.forename: S
authors.initials: S
authors.lastname: Pautz
authors.forename: U
authors.initials: U
authors.lastname: Kulka
authors.forename: A A
authors.initials: AA
authors.lastname: Friedl
authors.forename: S
authors.initials: S
authors.lastname: Lehr
authors.forename: S
authors.initials: S
authors.lastname: Hornhardt
authors.forename: M
authors.initials: M
authors.lastname: Gomolka
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism* / Q000528:radiation effects*; D002460:Cell Line; D005720:Gamma Rays; D005786:Gene Expression Regulation / Q000528:radiation effects*; D006801:Humans; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics; D011836:Radiation Tolerance / Q000528:radiation effects*; D013997:Time Factors; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D011506:Proteins
keywords: 2D-DIGE; biomarker; radiation; radio-sensitivity