title: Arthrobacter ginsengisoli sp. nov., isolated from soil of a ginseng field.
abstract: A Gram-staining-positive, catalase-positive, oxidase-negative, non-motile, non-flagellate and rod-shaped bacterium, was designated as DCY81(T), and isolated from soil of a ginseng field in Pocheon province, Republic of Korea. The 16S rRNA gene sequence analysis revealed that strain DCY81(T) belonged to the genus Arthrobacter. Major fatty acid was anteiso-C15:0, while major polar lipids were diphosphatidyglycerol, phatidyglycerol, phosphatidylinositol, monogalactosyldiacylglycerol (GL1), and dimannosyldiacylglycerol (GL2). The dominant quinone was MK-9(H2). The peptidoglycan type was A3α with an L-Lys-L-Ala-L-Thr-L-Ala interpeptide bridge. The DNA-DNA hybridization relatedness between strain DCY81(T) and Arthrobacter siccitolerans LMG 27359(T) (98.2 %), Arthrobacter sulfonivorans JCM 13520(T) (97.81 %), Arthrobacter scleromae DSM 17756(T) (97.59 %), Arthrobacter oxydans KCTC 3383(T) (97.3 %) was 39.1 ± 0.2, 62.2 ± 1.6, 36.8 ± 1.1 and 48.3 ± 1.6 %, respectively which show that the genotypic separation of strain DCY81(T) from the closest reference strain of the genus Arthrobacter. The DNA G+C content was 65.2 mol%. The genotypic analysis, physiological, and chemotaxonomic results indicate that strain DCY81(T) represents a novel species of the genus Arthrobacter. Therefore, Arthrobacter ginsengisoli sp. nov., is proposed as the type strain (=KCTC 29225(T) = JCM 19357(T)).
authors.affiliation: Graduate School of Biotechnology, Ginseng Genetic Resource Bank College of Life Science, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Muhammad Zubair
authors.initials: MZ
authors.lastname: Siddiqi
authors.forename: Yeon-Ju
authors.initials: YJ
authors.lastname: Kim
authors.forename: Van-An
authors.initials: VA
authors.lastname: Hoang
authors.forename: Muhammad Hanif
authors.initials: MH
authors.lastname: Siddiqi
authors.forename: Md Amdadul
authors.initials: MA
authors.lastname: Huq
authors.forename: Deok-Chun
authors.initials: DC
authors.lastname: Yang
mesh_terms: D001173:Arthrobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D005894:Panax; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D008055:Lipids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S