title: Expression of CTRP3, a novel adipokine, in rats at different pathogenic stages of type 2 diabetes mellitus and the impacts of GLP-1 receptor agonist on it.
abstract: This study aimed to investigate the expression of C1q/TNF-related protein-3 (CTRP3) in rats at different pathogenic stages of type 2 diabetes mellitus (T2DM) and the impacts of glucagon-like peptide-1 (GLP-1) receptor agonist on it. Male wistar rats were fed with high-fat diet for 10 weeks to induce insulin resistance (IR) and then were given low-dose streptozotocin (STZ) intraperitoneal injection to induce T2DM. Exendin-4 (Ex-4), a GLP-1 receptor agonist, was subcutaneous injected to the IR rats and T2DM rats for 4 weeks. The expression of CTRP3 mRNA and protein in epididymis adipose tissue of rats at the stage of IR was lower significantly than that of normal control (NC) rats and decreased more when they were at the stage of overt T2DM (all P < 0.05 or P < 0.01). After the treatment with Ex-4, the mRNA and protein expressions of CTRP3 were increased by 15.5% (P < 0.01) and 14.8% (P < 0.05), respectively, in IR rats and increased by 20.6% (P < 0.01) and 16.5% (P < 0.05), respectively, in T2DM rats. Overall, this study found that the expression of CTRP3 in visceral adipose tissue was progressively decreased in a T2DM rat model from the pathogenic stage of IR to overt diabetes, while Ex-4 treatment increased its expression in such animals.
authors.affiliation: Department of Endocrinology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Internal Medicine, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Endocrinology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Endocrinology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.
authors.forename: Yu-wen
authors.initials: YW
authors.lastname: Wu
authors.affiliation: Department of Endocrinology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.
authors.forename: Su-xin
authors.initials: SX
authors.lastname: Sun
authors.affiliation: Department of Endocrinology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.
authors.forename: Jia-zhong
authors.initials: JZ
authors.lastname: Sun
mesh_terms: D054392:Adipokines / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D059305:Diet, High-Fat; D018450:Disease Progression; D000077270:Exenatide; D005786:Gene Expression Regulation; D000067757:Glucagon-Like Peptide-1 Receptor; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007333:Insulin Resistance; D050152:Intra-Abdominal Fat / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010455:Peptides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D018027:Receptors, Glucagon / Q000819:agonists* / Q000378:metabolism; D013311:Streptozocin; D013997:Time Factors; D014688:Venoms / Q000494:pharmacology*
chemical_list: D054392:Adipokines; C000590534:C1q tumor necrosis factor-related protein 3, rat; C000593835:Glp1r protein, rat; D000067757:Glucagon-Like Peptide-1 Receptor; D007004:Hypoglycemic Agents; D010455:Peptides; D012333:RNA, Messenger; D018027:Receptors, Glucagon; D014688:Venoms; D013311:Streptozocin; D000077270:Exenatide