title: Corneal epithelial wound healing promoted by verbascoside-based liposomal eyedrops.
abstract: Different liposomal formulations were prepared to identify those capable of forming eyedrops for corneal diseases. Liposomes with neutral or slightly positive surface charge interact very well with the cornea. Then these formulations were loaded with verbascoside to heal a burn of corneal epithelium induced by alkali. The cornea surface affected involved in wound was monitored as a function of time. Experimental results were modeled by balance equation between the rate of healing, due to the flow of phenylpropanoid, and growth of the wound. The results indicate a latency time of only three hours and furthermore the corneal epithelium heals in 48 hours. Thus, the topical administration of verbascoside appears to reduce the action time of cells, as verified by histochemical and immunofluorescence assays.
authors.affiliation: Department of Bioscience and Territory (DIBT), University of Molise, Contrada Lappone, Pesche, 86090 Isernia, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Ambrosone
authors.affiliation: Department of Medicine and Health Sciences, University of Molise, Via F. De Sanctis, 86100 Campobasso, Italy.
authors.forename: Germano
authors.initials: G
authors.lastname: Guerra
authors.affiliation: Department of Public Health, University of Naples Federico II, Via S. Pansini 5, 80131 Naples, Italy.
authors.forename: Mariapia
authors.initials: M
authors.lastname: Cinelli
authors.affiliation: Department of Medicine and Health Sciences, University of Molise, Via F. De Sanctis, 86100 Campobasso, Italy.
authors.forename: Mariaelena
authors.initials: M
authors.lastname: Filippelli
authors.affiliation: Department of Bioscience and Territory (DIBT), University of Molise, Contrada Lappone, Pesche, 86090 Isernia, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Mosca
authors.affiliation: Department of Agriculture, Environment and Food (AAA), University of Molise, Via F. De Sanctis, 86100 Campobasso, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Vizzarri
authors.affiliation: Department of Medicine and Health Sciences, University of Molise, Via F. De Sanctis, 86100 Campobasso, Italy.
authors.forename: Dario
authors.initials: D
authors.lastname: Giorgio
authors.affiliation: Department of Medicine and Health Sciences, University of Molise, Via F. De Sanctis, 86100 Campobasso, Italy.
authors.forename: Ciro
authors.initials: C
authors.lastname: Costagliola
mesh_terms: D000818:Animals; D003316:Corneal Diseases / Q000188:drug therapy*; D019573:Epithelium, Corneal / Q000187:drug effects / Q000473:pathology; D005960:Glucosides / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D008081:Liposomes / Q000008:administration & dosage / Q000737:chemistry; D009883:Ophthalmic Solutions / Q000008:administration & dosage* / Q000737:chemistry; D010636:Phenols / Q000008:administration & dosage* / Q000737:chemistry; D011817:Rabbits; D014945:Wound Healing / Q000187:drug effects*; D059808:Polyphenols
chemical_list: D005960:Glucosides; D008081:Liposomes; D009883:Ophthalmic Solutions; D010636:Phenols; C058956:acteoside; D059808:Polyphenols