title: Gamma-cross-linked nonfibrillar collagen gel as a scaffold for osteogenic differentiation of mesenchymal stem cells.
abstract: We fabricated a transparent nonfibrillar collagen gel using gamma irradiation (5 kGy) and cultured rat mesenchymal stem cells (MSCs) on both the gamma-irradiated collagen gel and on unirradiated fibrillar collagen gel. Cells attached well and proliferated with high viability on the surface of both gels. The cells cultured on the gamma-irradiated nonfibrillar gel had a unique elongated shape and adhered to each other in culture. After 21 days of culture in dexamethasone-containing culture medium, the contents of bone-specific osteocalcin and calcium on the gamma-irradiated nonfibrillar gel were 1.4 and 1.9 times higher than those on fibrillar collagen gel, respectively. These data show that osteogenic differentiation of MSCs was promoted more efficiently on the gamma-cross-linked nonfibrillar gel than on the fibrillar gel and demonstrate the potential of the gamma-irradiated collagen gel for use in bone tissue engineering.
authors.affiliation: Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Takitoh
authors.affiliation: International Center for Research and Education on Mineral and Energy Resources, Akita University, 1-1 Tegata, Gakuen-machi, Akita, Akita 010-8502, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Bessho
authors.affiliation: Human Life Technology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Motohiro
authors.initials: M
authors.lastname: Hirose
authors.affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-46 Nakouji, Amagasaki, Hyogo 661-0974, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Ohgushi
authors.affiliation: Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Mori
authors.affiliation: Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan. Electronic address: hara@b.s.osakafu-u.ac.jp.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hara
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000737:chemistry / Q000166:cytology; D002118:Calcium / Q000032:analysis / Q000378:metabolism; D002448:Cell Adhesion; D048430:Cell Shape; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D024022:Fibrillar Collagens / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D005720:Gamma Rays; D005782:Gels; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D024041:Non-Fibrillar Collagens / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D015675:Osteocalcin / Q000032:analysis; D010012:Osteogenesis; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D024022:Fibrillar Collagens; D005782:Gels; D024041:Non-Fibrillar Collagens; D015675:Osteocalcin; D003907:Dexamethasone; D002118:Calcium
keywords: Bone formation; Bone marrow stromal cell; Collagen fibril; Mesenchymal stem cell; Osteoblast