title: Generation of dipeptidyl peptidase-IV-inhibiting peptides from β-lactoglobulin secreted by Lactococcus lactis.
abstract: Previous studies showed that hydrolysates of β-lactoglobulin (BLG) prepared using gastrointestinal proteases strongly inhibit dipeptidyl peptidase-IV (DPP-IV) activity in vitro. In this study, we developed a BLG-secreting Lactococcus lactis strain as a delivery vehicle and in situ expression system. Interestingly, trypsin-digested recombinant BLG from L. lactis inhibited DPP-IV activity, suggesting that BLG-secreting L. lactis may be useful in the treatment of type 2 diabetes mellitus.
authors.affiliation: Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan ; Japan Society for the Promotion of Science (JSPS), Japan.
authors.forename: Suguru
authors.initials: S
authors.lastname: Shigemori
authors.affiliation: Graduate School of Agriculture, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan.
authors.forename: Kazushi
authors.initials: K
authors.lastname: Oshiro
authors.affiliation: Graduate School of Agriculture, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Wang
authors.affiliation: Graduate School of Agriculture, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan.
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Yamamoto
authors.affiliation: Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan.
authors.forename: Yeqin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Internal Medicine and Clinical Immunology, Graduate School of Medicine, Yokohama City University, 3-9 Fukuura Kanagawa, Yokohama 236-0004, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Sato
authors.affiliation: Frontier Agriscience and Technology Center (FAST), Shinshu University, 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Uyeno
authors.affiliation: Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan ; Graduate School of Agriculture, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan ; Graduate School of Agriculture, Department of Interdisciplinary Genome Sciences and Cell Metabolism, Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research (ICCER), Shinshu University, 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shimosato
mesh_terms: D000818:Animals; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism*; D054873:Dipeptidyl-Peptidase IV Inhibitors / Q000494:pharmacology*; D005260:Female; D005822:Genetic Vectors / Q000378:metabolism; D013294:Lactococcus lactis / Q000378:metabolism*; D007782:Lactoglobulins / Q000737:chemistry* / Q000302:isolation & purification; D008807:Mice, Inbred BALB C; D009561:Nisin / Q000032:analysis; D010455:Peptides / Q000494:pharmacology*; D014357:Trypsin / Q000378:metabolism
chemical_list: D054873:Dipeptidyl-Peptidase IV Inhibitors; D007782:Lactoglobulins; D010455:Peptides; D009561:Nisin; D018819:Dipeptidyl Peptidase 4; D014357:Trypsin