title: Identification of murine basophils by flow cytometry and histology.
abstract: Here we describe how murine basophils can be detected in vivo by flow cytometry and immunofluorescence staining. Basophils constitute a homogeneous population of CD4(-)CD19(-)CD49b(+)IgE(+) cells in flow cytometric analysis. When IgE levels are low one can also use anti-FcεRI or anti-CD200R3 antibodies instead of anti-IgE. For immunofluorescence staining we use an anti-Mcpt8 antibody since Mcpt8 is a specific marker for murine basophils. We describe how to prepare the tissue to cut cryo-sections and how to perform the staining using a tyramide-based amplification kit.
authors.affiliation: Department of Infection Biology, University Clinic Erlangen and Friedrich-Alexander-University Erlangen-Nuremberg (FAU), Wasserturmstraße 3/5, Erlangen, 91054, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Schwartz
authors.forename: David
authors.initials: D
authors.lastname: Voehringer
mesh_terms: D000818:Animals; D001491:Basophils / Q000166:cytology*; D016616:Cryoultramicrotomy / Q000379:methods; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique / Q000379:methods; D006652:Histological Techniques / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology; D013194:Staining and Labeling / Q000379:methods