title: Preparation and cytotoxicity of N,N,N-trimethyl chitosan/alginate beads containing gold nanoparticles.
abstract: Polyelectrolyte complex beads based on N,N,N-trimethyl chitosan (TMC) and sodium alginate (ALG) were obtained. This biomaterial was characterised by FTIR, TGA/DTG, DSC and SEM analysis. The good properties of polyelectrolyte complex hydrogel beads were associated, for the first time, with gold nanoparticles (AuNPs). Through a straightforward methodology, AuNPs were encapsulated into the beads. The in vitro cytotoxicity assays on the Caco-2 colon cancer cells and healthy VERO cells showed that the beads presented good biocompatibility on both cell lines, whereas the beads loaded with gold nanoparticles (beads/AuNPs) was slightly cytotoxic on the Caco-2 and VERO cells.
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, GMPC-Departamento de Química, Universidade Estadual de Maringá UEM, Av. Colombo 5790, CEP 87020-900 Maringá, Paraná, Brazil; Universidade Tecnológica Federal do Paraná (UTFPR), Estrada para Boa Esperança, CEP 85660-000 Dois Vizinhos, Paraná, Brasil. Electronic address: afmartins50@yahoo.com.br.
authors.forename: Alessandro F
authors.initials: AF
authors.lastname: Martins
authors.affiliation: Universidade Tecnológica Federal do Paraná (UTFPR), Estrada para Boa Esperança, CEP 85660-000 Dois Vizinhos, Paraná, Brasil.
authors.forename: Suelen P
authors.initials: SP
authors.lastname: Facchi
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, GMPC-Departamento de Química, Universidade Estadual de Maringá UEM, Av. Colombo 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Johny P
authors.initials: JP
authors.lastname: Monteiro
authors.affiliation: Laboratório de Inovação Tecnológica no Desenvolvimento de Fármacos e Cosméticos-Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá UEM, Av. Colombo 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Samara R
authors.initials: SR
authors.lastname: Nocchi
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, GMPC-Departamento de Química, Universidade Estadual de Maringá UEM, Av. Colombo 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Cleiser T P
authors.initials: CT
authors.lastname: Silva
authors.affiliation: Laboratório de Inovação Tecnológica no Desenvolvimento de Fármacos e Cosméticos-Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá UEM, Av. Colombo 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Celso V
authors.initials: CV
authors.lastname: Nakamura
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, GMPC-Departamento de Química, Universidade Estadual de Maringá UEM, Av. Colombo 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Emerson M
authors.initials: EM
authors.lastname: Girotto
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, GMPC-Departamento de Química, Universidade Estadual de Maringá UEM, Av. Colombo 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Adley F
authors.initials: AF
authors.lastname: Rubira
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, GMPC-Departamento de Química, Universidade Estadual de Maringá UEM, Av. Colombo 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Edvani C
authors.initials: EC
authors.lastname: Muniz
mesh_terms: D000464:Alginates / Q000008:administration & dosage / Q000737:chemistry*; D000818:Animals; D018938:Caco-2 Cells; D048271:Chitosan / Q000008:administration & dosage / Q000009:adverse effects / Q000737:chemistry*; D002522:Chlorocebus aethiops; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry*; D020723:Glucuronic Acid / Q000008:administration & dosage / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006603:Hexuronic Acids / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D014709:Vero Cells
chemical_list: D000464:Alginates; D004337:Drug Carriers; D006603:Hexuronic Acids; D006046:Gold; D020723:Glucuronic Acid; D048271:Chitosan
keywords: Alginate; Beads; Gold nanoparticles; N,N,N-trimethyl chitosan; Polyelectrolyte complex