title: Probing ambiguous base-pairs by genetic transformation with XNA templates.
abstract: The templating potential of anhydrohexitol oligonucleotides bearing ambiguous bases was studied in vivo, by using a selection screen for mosaic heteroduplex plasmids in Escherichia coli. 1,5-Anhydro-2,3-dideoxy-2-(5-nitroindazol-1-yl)-D-arabino-hexitol showed the greatest ambiguity among the three nucleosides tested. At most two successive ambiguous bases could be tolerated on hexitol templates read in bacterial cells. Hexitol nucleosides bearing simplified heterocycles thus stand as promising monomers for generating random DNA sequences in vivo from defined synthetic oligonucleotides.
authors.affiliation: CEA, DSV, IG, Genoscope, 2 rue Gaston Crémieux 91057 Evry Cedex (France); ISSB, Génopole genavenir6, Equipe Xénome, 5 rue Henri Desbruères 91030 Evry Cedex (France).
authors.forename: Valérie
authors.initials: V
authors.lastname: Pezo
authors.forename: Guy
authors.initials: G
authors.lastname: Schepers
authors.forename: Catia
authors.initials: C
authors.lastname: Lambertucci
authors.forename: Philippe
authors.initials: P
authors.lastname: Marlière
authors.forename: Piet
authors.initials: P
authors.lastname: Herdewijn
mesh_terms: D020029:Base Pairing; D015394:Molecular Structure; D009696:Nucleic Acids / Q000737:chemistry / Q000235:genetics*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D013402:Sugar Alcohols / Q000737:chemistry*; D013698:Templates, Genetic; D014170:Transformation, Genetic / Q000235:genetics*
chemical_list: D009696:Nucleic Acids; D009841:Oligonucleotides; D013402:Sugar Alcohols; C543192:hexitol
keywords: HNA; XNA; mutagenesis; polymerization; universal bases; unnatural bases