title: FRIENDLY regulates mitochondrial distribution, fusion, and quality control in Arabidopsis.
abstract: Mitochondria are defining components of most eukaryotes. However, higher plant mitochondria differ biochemically, morphologically, and dynamically from those in other eukaryotes. FRIENDLY, a member of the CLUSTERED MITOCHONDRIA superfamily, is conserved among eukaryotes and is required for correct distribution of mitochondria within the cell. We sought to understand how disruption of FRIENDLY function in Arabidopsis (Arabidopsis thaliana) leads to mitochondrial clustering and the effects of this aberrant chondriome on cell and whole-plant physiology. We present evidence for a role of FRIENDLY in mediating intermitochondrial association, which is a necessary prelude to mitochondrial fusion. We demonstrate that disruption of mitochondrial association, motility, and chondriome structure in friendly affects mitochondrial quality control and leads to mitochondrial stress, cell death, and strong growth phenotypes.
authors.affiliation: Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E2 (A.M.E.Z., K.W., D.C.L.);Faculty of Science, Damanhour University, Damanhour 22516, Egypt (A.M.E.Z.);Institute of Crop Science and Resource Conservation, University of Bonn, D-53113 Bonn, Germany (M.S., A.J.M.);Max-Planck-Institute for Plant Breeding Research, Plant Proteomics Group, 50829 Cologne, Germany (I.F.);Department of Mathematics, Imperial College London, London SW7 2AZ, United Kingdom (I.G.J., C.G., N.S.J.);Université d'Angers, Institut National de la Recherche Agronomique, and Agrocampus Ouest, Unité Mixte de Recherche 1345, Institut de Recherche en Horticulture et Semences, Angers F-49045, France (A.B., D.M., D.C.L.); andPlant Biotechnology Institute, National Research Council of Canada, Saskatoon, Saskatchewan S7N 0W9, Canada (Y.C., R.D.).
authors.forename: Amr M
authors.initials: AM
authors.lastname: El Zawily
authors.affiliation: Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E2 (A.M.E.Z., K.W., D.C.L.);Faculty of Science, Damanhour University, Damanhour 22516, Egypt (A.M.E.Z.);Institute of Crop Science and Resource Conservation, University of Bonn, D-53113 Bonn, Germany (M.S., A.J.M.);Max-Planck-Institute for Plant Breeding Research, Plant Proteomics Group, 50829 Cologne, Germany (I.F.);Department of Mathematics, Imperial College London, London SW7 2AZ, United Kingdom (I.G.J., C.G., N.S.J.);Université d'Angers, Institut National de la Recherche Agronomique, and Agrocampus Ouest, Unité Mixte de Recherche 1345, Institut de Recherche en Horticulture et Semences, Angers F-49045, France (A.B., D.M., D.C.L.); andPlant Biotechnology Institute, National Research Council of Canada, Saskatoon, Saskatchewan S7N 0W9, Canada (Y.C., R.D.).
authors.forename: Markus
authors.initials: M
authors.lastname: Schwarzländer
authors.affiliation: Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E2 (A.M.E.Z., K.W., D.C.L.);Faculty of Science, Damanhour University, Damanhour 22516, Egypt (A.M.E.Z.);Institute of Crop Science and Resource Conservation, University of Bonn, D-53113 Bonn, Germany (M.S., A.J.M.);Max-Planck-Institute for Plant Breeding Research, Plant Proteomics Group, 50829 Cologne, Germany (I.F.);Department of Mathematics, Imperial College London, London SW7 2AZ, United Kingdom (I.G.J., C.G., N.S.J.);Université d'Angers, Institut National de la Recherche Agronomique, and Agrocampus Ouest, Unité Mixte de Recherche 1345, Institut de Recherche en Horticulture et Semences, Angers F-49045, France (A.B., D.M., D.C.L.); andPlant Biotechnology Institute, National Research Council of Canada, Saskatoon, Saskatchewan S7N 0W9, Canada (Y.C., R.D.).
authors.forename: Iris
authors.initials: I
authors.lastname: Finkemeier
authors.affiliation: Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E2 (A.M.E.Z., K.W., D.C.L.);Faculty of Science, Damanhour University, Damanhour 22516, Egypt (A.M.E.Z.);Institute of Crop Science and Resource Conservation, University of Bonn, D-53113 Bonn, Germany (M.S., A.J.M.);Max-Planck-Institute for Plant Breeding Research, Plant Proteomics Group, 50829 Cologne, Germany (I.F.);Department of Mathematics, Imperial College London, London SW7 2AZ, United Kingdom (I.G.J., C.G., N.S.J.);Université d'Angers, Institut National de la Recherche Agronomique, and Agrocampus Ouest, Unité Mixte de Recherche 1345, Institut de Recherche en Horticulture et Semences, Angers F-49045, France (A.B., D.M., D.C.L.); andPlant Biotechnology Institute, National Research Council of Canada, Saskatoon, Saskatchewan S7N 0W9, Canada (Y.C., R.D.).
authors.forename: Iain G
authors.initials: IG
authors.lastname: Johnston
authors.affiliation: Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E2 (A.M.E.Z., K.W., D.C.L.);Faculty of Science, Damanhour University, Damanhour 22516, Egypt (A.M.E.Z.);Institute of Crop Science and Resource Conservation, University of Bonn, D-53113 Bonn, Germany (M.S., A.J.M.);Max-Planck-Institute for Plant Breeding Research, Plant Proteomics Group, 50829 Cologne, Germany (I.F.);Department of Mathematics, Imperial College London, London SW7 2AZ, United Kingdom (I.G.J., C.G., N.S.J.);Université d'Angers, Institut National de la Recherche Agronomique, and Agrocampus Ouest, Unité Mixte de Recherche 1345, Institut de Recherche en Horticulture et Semences, Angers F-49045, France (A.B., D.M., D.C.L.); andPlant Biotechnology Institute, National Research Council of Canada, Saskatoon, Saskatchewan S7N 0W9, Canada (Y.C., R.D.).
authors.forename: Abdelilah
authors.initials: A
authors.lastname: Benamar
authors.affiliation: Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E2 (A.M.E.Z., K.W., D.C.L.);Faculty of Science, Damanhour University, Damanhour 22516, Egypt (A.M.E.Z.);Institute of Crop Science and Resource Conservation, University of Bonn, D-53113 Bonn, Germany (M.S., A.J.M.);Max-Planck-Institute for Plant Breeding Research, Plant Proteomics Group, 50829 Cologne, Germany (I.F.);Department of Mathematics, Imperial College London, London SW7 2AZ, United Kingdom (I.G.J., C.G., N.S.J.);Université d'Angers, Institut National de la Recherche Agronomique, and Agrocampus Ouest, Unité Mixte de Recherche 1345, Institut de Recherche en Horticulture et Semences, Angers F-49045, France (A.B., D.M., D.C.L.); andPlant Biotechnology Institute, National Research Council of Canada, Saskatoon, Saskatchewan S7N 0W9, Canada (Y.C., R.D.).
authors.forename: Yongguo
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E2 (A.M.E.Z., K.W., D.C.L.);Faculty of Science, Damanhour University, Damanhour 22516, Egypt (A.M.E.Z.);Institute of Crop Science and Resource Conservation, University of Bonn, D-53113 Bonn, Germany (M.S., A.J.M.);Max-Planck-Institute for Plant Breeding Research, Plant Proteomics Group, 50829 Cologne, Germany (I.F.);Department of Mathematics, Imperial College London, London SW7 2AZ, United Kingdom (I.G.J., C.G., N.S.J.);Université d'Angers, Institut National de la Recherche Agronomique, and Agrocampus Ouest, Unité Mixte de Recherche 1345, Institut de Recherche en Horticulture et Semences, Angers F-49045, France (A.B., D.M., D.C.L.); andPlant Biotechnology Institute, National Research Council of Canada, Saskatoon, Saskatchewan S7N 0W9, Canada (Y.C., R.D.).
authors.forename: Clémence
authors.initials: C
authors.lastname: Gissot
authors.affiliation: Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E2 (A.M.E.Z., K.W., D.C.L.);Faculty of Science, Damanhour University, Damanhour 22516, Egypt (A.M.E.Z.);Institute of Crop Science and Resource Conservation, University of Bonn, D-53113 Bonn, Germany (M.S., A.J.M.);Max-Planck-Institute for Plant Breeding Research, Plant Proteomics Group, 50829 Cologne, Germany (I.F.);Department of Mathematics, Imperial College London, London SW7 2AZ, United Kingdom (I.G.J., C.G., N.S.J.);Université d'Angers, Institut National de la Recherche Agronomique, and Agrocampus Ouest, Unité Mixte de Recherche 1345, Institut de Recherche en Horticulture et Semences, Angers F-49045, France (A.B., D.M., D.C.L.); andPlant Biotechnology Institute, National Research Council of Canada, Saskatoon, Saskatchewan S7N 0W9, Canada (Y.C., R.D.).
authors.forename: Andreas J
authors.initials: AJ
authors.lastname: Meyer
authors.affiliation: Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E2 (A.M.E.Z., K.W., D.C.L.);Faculty of Science, Damanhour University, Damanhour 22516, Egypt (A.M.E.Z.);Institute of Crop Science and Resource Conservation, University of Bonn, D-53113 Bonn, Germany (M.S., A.J.M.);Max-Planck-Institute for Plant Breeding Research, Plant Proteomics Group, 50829 Cologne, Germany (I.F.);Department of Mathematics, Imperial College London, London SW7 2AZ, United Kingdom (I.G.J., C.G., N.S.J.);Université d'Angers, Institut National de la Recherche Agronomique, and Agrocampus Ouest, Unité Mixte de Recherche 1345, Institut de Recherche en Horticulture et Semences, Angers F-49045, France (A.B., D.M., D.C.L.); andPlant Biotechnology Institute, National Research Council of Canada, Saskatoon, Saskatchewan S7N 0W9, Canada (Y.C., R.D.).
authors.forename: Ken
authors.initials: K
authors.lastname: Wilson
authors.affiliation: Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E2 (A.M.E.Z., K.W., D.C.L.);Faculty of Science, Damanhour University, Damanhour 22516, Egypt (A.M.E.Z.);Institute of Crop Science and Resource Conservation, University of Bonn, D-53113 Bonn, Germany (M.S., A.J.M.);Max-Planck-Institute for Plant Breeding Research, Plant Proteomics Group, 50829 Cologne, Germany (I.F.);Department of Mathematics, Imperial College London, London SW7 2AZ, United Kingdom (I.G.J., C.G., N.S.J.);Université d'Angers, Institut National de la Recherche Agronomique, and Agrocampus Ouest, Unité Mixte de Recherche 1345, Institut de Recherche en Horticulture et Semences, Angers F-49045, France (A.B., D.M., D.C.L.); andPlant Biotechnology Institute, National Research Council of Canada, Saskatoon, Saskatchewan S7N 0W9, Canada (Y.C., R.D.).
authors.forename: Raju
authors.initials: R
authors.lastname: Datla
authors.affiliation: Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E2 (A.M.E.Z., K.W., D.C.L.);Faculty of Science, Damanhour University, Damanhour 22516, Egypt (A.M.E.Z.);Institute of Crop Science and Resource Conservation, University of Bonn, D-53113 Bonn, Germany (M.S., A.J.M.);Max-Planck-Institute for Plant Breeding Research, Plant Proteomics Group, 50829 Cologne, Germany (I.F.);Department of Mathematics, Imperial College London, London SW7 2AZ, United Kingdom (I.G.J., C.G., N.S.J.);Université d'Angers, Institut National de la Recherche Agronomique, and Agrocampus Ouest, Unité Mixte de Recherche 1345, Institut de Recherche en Horticulture et Semences, Angers F-49045, France (A.B., D.M., D.C.L.); andPlant Biotechnology Institute, National Research Council of Canada, Saskatoon, Saskatchewan S7N 0W9, Canada (Y.C., R.D.).
authors.forename: David
authors.identifier: http://orcid.org/0000-0002-3352-2185
authors.initials: D
authors.lastname: Macherel
authors.affiliation: Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E2 (A.M.E.Z., K.W., D.C.L.);Faculty of Science, Damanhour University, Damanhour 22516, Egypt (A.M.E.Z.);Institute of Crop Science and Resource Conservation, University of Bonn, D-53113 Bonn, Germany (M.S., A.J.M.);Max-Planck-Institute for Plant Breeding Research, Plant Proteomics Group, 50829 Cologne, Germany (I.F.);Department of Mathematics, Imperial College London, London SW7 2AZ, United Kingdom (I.G.J., C.G., N.S.J.);Université d'Angers, Institut National de la Recherche Agronomique, and Agrocampus Ouest, Unité Mixte de Recherche 1345, Institut de Recherche en Horticulture et Semences, Angers F-49045, France (A.B., D.M., D.C.L.); andPlant Biotechnology Institute, National Research Council of Canada, Saskatoon, Saskatchewan S7N 0W9, Canada (Y.C., R.D.).
authors.forename: Nick S
authors.initials: NS
authors.lastname: Jones
authors.affiliation: Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E2 (A.M.E.Z., K.W., D.C.L.);Faculty of Science, Damanhour University, Damanhour 22516, Egypt (A.M.E.Z.);Institute of Crop Science and Resource Conservation, University of Bonn, D-53113 Bonn, Germany (M.S., A.J.M.);Max-Planck-Institute for Plant Breeding Research, Plant Proteomics Group, 50829 Cologne, Germany (I.F.);Department of Mathematics, Imperial College London, London SW7 2AZ, United Kingdom (I.G.J., C.G., N.S.J.);Université d'Angers, Institut National de la Recherche Agronomique, and Agrocampus Ouest, Unité Mixte de Recherche 1345, Institut de Recherche en Horticulture et Semences, Angers F-49045, France (A.B., D.M., D.C.L.); andPlant Biotechnology Institute, National Research Council of Canada, Saskatoon, Saskatchewan S7N 0W9, Canada (Y.C., R.D.) david.logan@univ-angers.fr.
authors.forename: David C
authors.identifier: http://orcid.org/0000-0002-8980-240X
authors.initials: DC
authors.lastname: Logan
mesh_terms: D000199:Actins / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D003599:Cytoskeleton / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000378:metabolism*; D010788:Photosynthesis; D059467:Transcriptome
chemical_list: D000199:Actins; D029681:Arabidopsis Proteins