title: Isolation of leukocytes from mouse central nervous system.
abstract: During neuroinflammatory or neurodegenerative diseases, it is often critical to characterize the composition of infiltrating immune cells. This protocol describes a reliable, fast, and simple method for the isolation of leukocytes from murine central nervous system (CNS) during steady state or inflammation for analysis by flow cytometry or other techniques.
authors.affiliation: Institute of Experimental Immunology, University of Zürich, Winterthurerstrasse 190, 8057, Zürich, Switzerland.
authors.forename: Iva
authors.initials: I
authors.lastname: Lelios
authors.forename: Melanie
authors.initials: M
authors.lastname: Greter
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods; D002490:Central Nervous System / Q000166:cytology* / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology / Q000473:pathology; D004660:Encephalitis / Q000276:immunology / Q000473:pathology; D007962:Leukocytes / Q000166:cytology* / Q000473:pathology; D008214:Lymphocytes / Q000473:pathology; D008264:Macrophages / Q000166:cytology / Q000473:pathology; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000473:pathology; D009000:Monocytes / Q000473:pathology; D009504:Neutrophils / Q000473:pathology