title: Difference in Early Activation of NF-κB and MCP-1 in Acinar-Cell-Rich versus Fibrotic Human Pancreas Exposed to Surgical Trauma and Hypoxia.
abstract: Objectives. Previously we have shown that a pancreas with over 40% acinar cells is exposed to postoperative pancreatitis and other complications after pancreaticoduodenectomy (PD). Our aim was to analyze the expression of NF-κB and MCP-1 in the cut edge of human pancreas after PD in both acinar-cell-rich and fibrotic pancreata. Methods. Several pancreatic samples from six patients, three with acinar-cell-rich and three with fibrotic pancreata, were exposed to surgical trauma in PD, and thereafter to hypoxemia for 15 minutes, 2-2.5 hours, 4 hours, or 6 hours, to mimic postoperative conditions of the pancreatic remnant in a patient. Immunohistochemical analysis of inflammation markers (NF-κB, MCP-1) was performed. Results. In the acinar-cell-rich pancreata, intra-acinar NF-κB and MCP-1 expression increased from mild at 15 minutes to high during the first 4 hours, whereas in ductal cells MCP-1 staining was highly intense at both time points. Acinar cell NF-κB and MCP-1 expression and ductal cell MCP-1 expression were also observed in the fibrotic pancreata, but the activation remained low throughout the 6 hours. Conclusions. In acinar-cell-rich pancreas, an extensive inflammatory cascade begins almost immediately after surgical trauma. Fibrosis may limit the progression of inflammatory process in pancreas.
authors.affiliation: Department of Gastroenterology and Alimentary Tract Surgery, Tampere Pancreas Laboratory, Tampere University Hospital, Teiskontie 35, 33521 Tampere, Finland.
authors.forename: Matias
authors.identifier: 0000-0002-8888-2743
authors.initials: M
authors.lastname: Laaninen
authors.affiliation: Department of Gastroenterology and Alimentary Tract Surgery, Tampere Pancreas Laboratory, Tampere University Hospital, Teiskontie 35, 33521 Tampere, Finland.
authors.forename: Merja
authors.initials: M
authors.lastname: Bläuer
authors.affiliation: Department of Gastroenterology and Alimentary Tract Surgery, Tampere Pancreas Laboratory, Tampere University Hospital, Teiskontie 35, 33521 Tampere, Finland.
authors.forename: Juhani
authors.initials: J
authors.lastname: Sand
authors.affiliation: Department of Gastroenterology and Alimentary Tract Surgery, Tampere Pancreas Laboratory, Tampere University Hospital, Teiskontie 35, 33521 Tampere, Finland.
authors.forename: Isto
authors.identifier: 0000-0002-0611-9905
authors.initials: I
authors.lastname: Nordback
authors.affiliation: Department of Gastroenterology and Alimentary Tract Surgery, Tampere Pancreas Laboratory, Tampere University Hospital, Teiskontie 35, 33521 Tampere, Finland.
authors.forename: Johanna
authors.initials: J
authors.lastname: Laukkarinen