title: Replication slippage of the thermophilic DNA polymerases B and D from the Euryarchaeota Pyrococcus abyssi.
abstract: Replication slippage or slipped-strand mispairing involves the misalignment of DNA strands during the replication of repeated DNA sequences, and can lead to genetic rearrangements such as microsatellite instability. Here, we show that PolB and PolD replicative DNA polymerases from the archaeal model Pyrococcus abyssi (Pab) slip in vitro during replication of a single-stranded DNA template carrying a hairpin structure and short direct repeats. We find that this occurs in both their wild-type (exo+) and exonuclease deficient (exo-) forms. The slippage behavior of PabPolB and PabPolD, probably due to limited strand displacement activity, resembles that observed for the high fidelity P. furiosus (Pfu) DNA polymerase. The presence of PabPCNA inhibited PabPolB and PabPolD slippage. We propose a model whereby PabPCNA stimulates strand displacement activity and polymerase progression through the hairpin, thus permitting the error-free replication of repetitive sequences.
authors.affiliation: Departamento de Biología Celular, Genética y Fisiología, Facultad de Ciencias, Universidad de Malaga Málaga, Spain.
authors.forename: Melissa
authors.initials: M
authors.lastname: Castillo-Lizardo
authors.affiliation: Laboratoire de Microbiologie des Environnements Extrêmes, UMR 6197, Institut Français de Recherche pour l'Exploitation de la Mer, Université de Bretagne Occidentale Plouzané, France ; CNRS, UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes Plouzané, France.
authors.forename: Ghislaine
authors.initials: G
authors.lastname: Henneke
authors.affiliation: Departamento de Biología Celular, Genética y Fisiología, Facultad de Ciencias, Universidad de Malaga Málaga, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Viguera
keywords: Archaea; DNA polymerases; primer-template misalignment; slippage; strand displacement activity