title: Recovery and Cultivation of Keratinocytes From Shipped Mouse Skin.
abstract: Murine keratinocyte culture from neonatal skin is an important tool for studying the functional role of specific genes in epithelial biology. However, when the transgenic animal is only available in a geographically distant local, obtaining viable keratinocytes can be problematic. A method for transferring the isolated murine skin from collaborating labs could decrease the cost of shipping live animals, and would allow the efficient use of the tissues from the transgenic animals. Here we optimized shipping conditions and characterized the cells retrieved and cultured from mouse skin shipped for 48 h at 0 °C. The cultured keratinocytes from the control, non-shipped skin and the 2-day shipped skin were 43.6 +/- 7.8% viable, doubled every 2 days, and expressed comparable amounts of heat shock proteins and CD29/integrin beta-1. However, under the same shipping conditions, the 3-day shipped tissue failed to establish colonies in the culture. Therefore, this 2-day shipping technique allows the transfer mouse skin from distant locations with recovery of viable, propagatable keratinocytes, facilitating long-distance collaborations.
authors.affiliation: Department of Dermatology, School of Medicine, University of California, Davis, California.
authors.forename: Hsin-Ya
authors.initials: HY
authors.lastname: Yang
authors.affiliation: Department of Dermatology, School of Medicine, University of California, Davis, California.
authors.forename: Thi Dinh
authors.initials: TD
authors.lastname: La
authors.affiliation: Department of Dermatology, School of Medicine, University of California, Davis, California.
authors.forename: Zhanna
authors.initials: Z
authors.lastname: Gurenko
authors.affiliation: Department of Dermatology, School of Medicine, University of California, Davis, California.
authors.forename: Pieter
authors.initials: P
authors.lastname: Steenhuis
authors.affiliation: Department of Dermatology, School of Medicine, University of California, Davis, California.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Dermatology, School of Medicine, University of California, Davis, California.
authors.forename: R Rivkah
authors.initials: RR
authors.lastname: Isseroff
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D019012:Integrin beta1 / Q000378:metabolism; D015603:Keratinocytes / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D009926:Organ Preservation; D020127:Recovery of Function / Q000502:physiology*; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D009927:Tissue and Organ Procurement / Q000379:methods
chemical_list: D019012:Integrin beta1