title: Function of Sox2 in ependymal cells of lesioned spinal cords in adult zebrafish.
abstract: The sex-determining region Y-box 2 (Sox2) is related not only to pluripotency, but also to cell proliferation. Zebrafish can regain their motor function after spinal cord injury (SCI). Following SCI, new motor neurons are produced from proliferating ependymal cells. Here, we investigated the expression and function of Sox2 after SCI in zebrafish. Sox2 was upregulated as early as 1 day post-lesion (dpl) in ependymal cells, which was followed by cell proliferation. Sox2 knockdown significantly decreased the number of proliferating cells at 5dpl. The results of this study suggest a role of Sox2 as one of the proliferation initiators in ependymal cells after SCI.
authors.affiliation: Department of Molecular Neurobiology, Graduate School of Medicine, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa 920-8640, Japan; Wellness Promotion Science Center, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa, Ishikawa 920-0942, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ogai
authors.affiliation: Department of Clinical Laboratory Science, Graduate School of Medical Science, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa, Ishikawa 920-0942, Japan.
authors.forename: Kumi
authors.initials: K
authors.lastname: Nakatani
authors.affiliation: Department of Clinical Laboratory Science, Graduate School of Medical Science, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa, Ishikawa 920-0942, Japan.
authors.forename: Suguru
authors.initials: S
authors.lastname: Hisano
authors.affiliation: Department of Clinical Laboratory Science, Graduate School of Medical Science, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa, Ishikawa 920-0942, Japan.
authors.forename: Kayo
authors.initials: K
authors.lastname: Sugitani
authors.affiliation: Department of Molecular Neurobiology, Graduate School of Medicine, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa 920-8640, Japan; Graduate School and Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science, 3500-3 Minamitamagaki, Suzuka, Mie 513-8670, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Koriyama
authors.affiliation: Department of Molecular Neurobiology, Graduate School of Medicine, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa 920-8640, Japan. Electronic address: satoru@med.kanazawa-u.ac.jp.
authors.forename: Satoru
authors.initials: S
authors.lastname: Kato
mesh_terms: D000375:Aging; D000818:Animals; D049109:Cell Proliferation / Q000502:physiology; D004195:Disease Models, Animal; D004805:Ependyma / Q000378:metabolism*; D009046:Motor Neurons / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism; D055747:SOX Transcription Factors / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000503:physiopathology; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000378:metabolism*
chemical_list: D055747:SOX Transcription Factors; C544611:Sox2 protein, zebrafish; D029961:Zebrafish Proteins
keywords: Cell proliferation; Sox2; Spinal cord regeneration; Zebrafish