title: Evaluation of Parkia pendula lectin mRNA differentially expressed in seedlings.
abstract: Parkia pendula (Willd.) Walp. (Fabaceae) is a neotropical species of the genus Parkia more abundantly distributed in Central to South America. From the seeds of P. pendula a glucose/mannose specific lectin (PpeL) was isolated that has been characterised and used as a biotechnological tool but until now this is the first manuscript to analyse P. pendula mRNA expression in seedlings. For this porpoise a Differential display reverse transcription polimerase chain reaction (DDRT-PCR) was used to evaluate the expression of P. pendula lectin mRNAs in non-rooted seedlings. No bands were observed in the agarose gel, indicating the absence of mRNA of PpeL seedlings. our findings confirm that lectins mRNAs are differently regulated among species even if they are grouped in the same class.
authors.affiliation: Laboratory of Immunopathology Keizo Asami, Department of Pathology, Federal University of Pernambuco ? UFPE, Recife, PE, Brazil.
authors.forename: M J B M
authors.initials: MJ
authors.lastname: Rêgo
authors.affiliation: Laboratory of Immunopathology Keizo Asami, Department of Pathology, Federal University of Pernambuco ? UFPE, Recife, PE, Brazil.
authors.forename: P B
authors.initials: PB
authors.lastname: Santos
authors.affiliation: Laboratory of Immunopathology Keizo Asami, Department of Pathology, Federal University of Pernambuco ? UFPE, Recife, PE, Brazil.
authors.forename: L B
authors.initials: LB
authors.lastname: Carvalho-Junior
authors.affiliation: Laboratory of Molecular Genetics, Division of Life Sciences, King's College London, London, United Kingdom.
authors.forename: J
authors.initials: J
authors.lastname: Stirling
authors.affiliation: Laboratory of Immunopathology Keizo Asami, Department of Pathology, Federal University of Pernambuco ? UFPE, Recife, PE, Brazil.
authors.forename: E I C
authors.initials: EI
authors.lastname: Beltrão
mesh_terms: D007887:Fabaceae / Q000737:chemistry / Q000235:genetics*; D037121:Plant Lectins / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D018749:RNA, Plant / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings
chemical_list: D037121:Plant Lectins; D012333:RNA, Messenger; D018749:RNA, Plant