title: Quantification of rilpivirine in human plasma, cervicovaginal fluid, rectal fluid and genital/rectal mucosal tissues using liquid chromatography-tandem mass spectrometry.
abstract: BACKGROUND
  A sensitive, specific and robust liquid chromatography-tandem mass spectrometry method has been developed and validated for the quantification of rilpivirine in human plasma, genital/rectal biofluids and mucosal tissues.
  
  
  METHODS
  Plasma and tissue samples were extracted using protein precipitation (acetonitrile/water; 5:1 v/v), and genital/rectal biofluids absorbed onto ophthalmic swabs were extracted using liquid-liquid extraction (hexane/ethyl acetate; 80:20 v/v). A stable isotope-labeled internal standard ((13)C-d4-RPV) was used, and the assay was validated over a concentration range of 0.5-400 ng/ml.
  
  
  CONCLUSIONS
  Inter- and intra-assay precision and accuracy met the acceptance as per US FDA bioanalytical guidelines. The validated assay has been used for the determination of rilpivirine concentrations in these matrices as part of an exploratory pharmacokinetic study investigating the suitability of a long-acting formulation of rilpivirine for pre-exposure prophylaxis.
authors.affiliation: Liverpool Bioanalytical Facility, Department of Molecular & Clinical Pharmacology, University of Liverpool, Liverpool, UK.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Else
authors.forename: John
authors.initials: J
authors.lastname: Tjia
authors.forename: Akil
authors.initials: A
authors.lastname: Jackson
authors.forename: Sujan Dilly
authors.initials: SD
authors.lastname: Penchala
authors.forename: Deirdre
authors.initials: D
authors.lastname: Egan
authors.forename: Marta
authors.initials: M
authors.lastname: Boffito
authors.forename: Saye H
authors.initials: SH
authors.lastname: Khoo
authors.forename: David J
authors.initials: DJ
authors.lastname: Back
mesh_terms: D019380:Anti-HIV Agents / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics*; D001826:Body Fluids / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D009092:Mucous Membrane / Q000378:metabolism; D009570:Nitriles / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics*; D011743:Pyrimidines / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics*; D015203:Reproducibility of Results; D000068696:Rilpivirine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D019380:Anti-HIV Agents; D009570:Nitriles; D011743:Pyrimidines; D000068696:Rilpivirine