title: Mycoplasma hyorhinis induces epithelial-mesenchymal transition in gastric cancer cell MGC803 via TLR4-NF-κB signaling.
abstract: Our previous works showed chronic infection of Mycoplasma hyorhinis (M. hyorhinis) was associated with gastric cancer metastasis, but the mechanisms were unknown. Herein, we found M. hyorhinis induced epithelial-mesenchymal transition (EMT) in gastric cancer cell MGC803, which was counteracted by inhibitor of NF-κB signaling or p65 knockdown. Furthermore, we found that TLR4 associated with p37, a membrane protein of M. hyorhinis. Knock-down or inhibition of TLR4 antagonized M. hyorhinis-induced NF-κB signaling, EMT, and cell migration. Thus, M. hyorhinis induces EMT and promotes cell migration via TLR4-NF-κB signaling, which provides a clue to the pathogenesis of M. hyorhinis in gastric cancer.
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, Peking University Cancer Hospital & Institute, Beijing 100142, China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Duan
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, Peking University Cancer Hospital & Institute, Beijing 100142, China.
authors.forename: Like
authors.initials: L
authors.lastname: Qu
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, Peking University Cancer Hospital & Institute, Beijing 100142, China. Electronic address: scc@bjcancer.org.
authors.forename: Chengchao
authors.initials: C
authors.lastname: Shou
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D006801:Humans; D009175:Mycoplasma Infections / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D045790:Mycoplasma hyorhinis / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism*; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D051197:Toll-Like Receptor 4 / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D016328:NF-kappa B; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; C553975:p37 protein, human
keywords: EMT; Gastric cancer; Mycoplasma hyorhinis; NF-κB; TLR4