title: A modified acidic approach for DNA extraction from plant species containing high levels of secondary metabolites.
abstract: Purified genomic DNA can be difficult to obtain from some plant species because of the presence of impurities such as polysaccharides, which are often co-extracted with DNA. In this study, we developed a fast, simple, and low-cost protocol for extracting DNA from plants containing high levels of secondary metabolites. This protocol does not require the use of volatile toxic reagents such as mercaptoethanol, chloroform, or phenol and allows the extraction of high-quality DNA from wild and cultivated tropical species.
authors.affiliation: Agência Paulista de Tecnologia dos Agronegócios, Polo Apta Centro-Sul, Piracicaba, SP, Brasil.
authors.forename: M M
authors.initials: MM
authors.lastname: Cavallari
authors.affiliation: Central de Laboratórios de Pesquisa, Ciência e Tecnologia Ambiental, Pró-Reitoria de Pesquisa e Pós-Graduação, Universidade do Sagrado Coração, Bauru, SP, Brasil mvbsiqueira@gmail.com.
authors.forename: M V B M
authors.initials: MV
authors.lastname: Siqueira
authors.affiliation: Laboratório de Diversidade Genética e Melhoramento, Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, SP, Brasil.
authors.forename: T M
authors.initials: TM
authors.lastname: Val
authors.affiliation: Agência Paulista de Tecnologia dos Agronegócios, Polo Apta Centro-Sul, Piracicaba, SP, Brasil.
authors.forename: J C
authors.initials: JC
authors.lastname: Pavanelli
authors.affiliation: Agência Paulista de Tecnologia dos Agronegócios, Polo Apta Centro-Sul, Piracicaba, SP, Brasil.
authors.forename: M
authors.initials: M
authors.lastname: Monteiro
authors.affiliation: Instituto de Biologia, Universidade Estadual de Campinas, Cidade Universitária Zeferino Vaz, Campinas, SP, Brasil.
authors.forename: C
authors.initials: C
authors.lastname: Grando
authors.affiliation: Laboratório de Diversidade Genética e Melhoramento, Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, SP, Brasil.
authors.forename: J B
authors.initials: JB
authors.lastname: Pinheiro
authors.affiliation: Agência Paulista de Tecnologia dos Agronegócios, Polo Apta Centro-Sul, Piracicaba, SP, Brasil.
authors.forename: M I
authors.initials: MI
authors.lastname: Zucchi
authors.affiliation: Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Brasília, DF, Brasil.
authors.forename: M A
authors.initials: MA
authors.lastname: Gimenes
mesh_terms: D031021:Anacardium / Q000737:chemistry* / Q000235:genetics; D002021:Buffers; D037821:Casearia / Q000737:chemistry* / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D006863:Hydrogen-Ion Concentration; D032411:Lippia / Q000737:chemistry* / Q000235:genetics; D059627:Liquid Phase Microextraction / Q000379:methods*; D018895:Microsatellite Repeats; D018515:Plant Leaves / Q000737:chemistry* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000592:standards; D059808:Polyphenols; D019347:Potassium Acetate; D013447:Sulfites
chemical_list: D002021:Buffers; D018744:DNA, Plant; D059808:Polyphenols; D013447:Sulfites; D019347:Potassium Acetate; C009279:sodium bisulfite