title: First evidence of TRPV5 and TRPV6 channels in human parathyroid glands: possible involvement in neoplastic transformation.
abstract: The parathyroid glands play an overall regulatory role in the systemic calcium (Ca(2+)) homeostasis. The purpose of the present study was to demonstrate the presence of the Ca(2+) channels transient receptor potential vanilloid (TRPV) 5 and TRPV6 in human parathyroid glands. Semi-quantitative and quantitative PCR was carried out to evaluate the presence of TRPV5 and TRPV6 mRNAs in sporadic parathyroid adenomas and normal parathyroid glands. Western blot and immunocytochemical assays were used to assess protein expression, cellular localization and time expression in primary cultures from human parathyroid adenoma. TRPV5 and TRPV6 transcripts were then identified both in normal and pathological tissues. Predominant immunoreactive bands were detected at 75-80 kD for both vanilloid channels. These channels co-localized with the calcium-sensing receptor (CASR) on the membrane surface, but immunoreactivity was also detected in the cytosol and around the nuclei. Our data showed that western blotting recorded an increase of protein expression of both channels in adenoma samples compared with normal glands suggesting a potential relation with the cell calcium signalling pathway and the pathological processes of these glands.
authors.affiliation: Department of Pharmacy, University of Pisa, Pisa, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Giusti
authors.forename: Filomena
authors.initials: F
authors.lastname: Cetani
authors.forename: Ylenia
authors.initials: Y
authors.lastname: Da Valle
authors.forename: Elena
authors.initials: E
authors.lastname: Pardi
authors.forename: Federica
authors.initials: F
authors.lastname: Ciregia
authors.forename: Elena
authors.initials: E
authors.lastname: Donadio
authors.forename: Claudia
authors.initials: C
authors.lastname: Gargini
authors.forename: Ilaria
authors.initials: I
authors.lastname: Piano
authors.forename: Simona
authors.initials: S
authors.lastname: Borsari
authors.forename: Ali
authors.initials: A
authors.lastname: Jaber
authors.forename: Antonella
authors.initials: A
authors.lastname: Caputo
authors.forename: Fulvio
authors.initials: F
authors.lastname: Basolo
authors.forename: Gino
authors.initials: G
authors.lastname: Giannaccini
authors.forename: Claudio
authors.initials: C
authors.lastname: Marcocci
authors.forename: Antonio
authors.initials: A
authors.lastname: Lucacchini
mesh_terms: D000236:Adenoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002478:Cells, Cultured; D006801:Humans; D049950:Hyperparathyroidism, Primary / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D007124:Immunoenzyme Techniques; D010280:Parathyroid Glands / Q000166:cytology / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D044169:Receptors, Calcium-Sensing / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism*
chemical_list: D015220:Calcium Channels; D012333:RNA, Messenger; D044169:Receptors, Calcium-Sensing; D050916:TRPV Cation Channels; C492239:TRPV5 protein, human; C422827:TRPV6 protein, human; D002118:Calcium
keywords: TRPV5; TRPV6; adenoma; calcium; parathyroid glands