title: The respiratory chains of four strains of the alkaliphilic Bacillus clausii.
abstract: A comparative analysis of terminal respiratory enzymes has been performed on four strains of Bacillus clausii used for preparation of a European probiotic. These four strains originated most probably from a common ancestor through early selection of stable clones for industrial propagation. They exhibit a low level of intra-specific diversity and a high degree of genomic conservation, making them an attractive model to study the different bioenergetics behaviors of alkaliphilic bacilli. The analysis of the different bioenergetics responses has been carried out revealing striking differences among the strains. Two out of the four strains have shown a functional redundancy of the terminal part of the respiratory chain. The biochemical data correlate with the expression level of the mRNA of cytochrome c oxidase and quinol oxidase genes (heme-copper type). The consequences of these different bioenergetics behaviors are also discussed.
authors.affiliation: SMBNOS, University of Bari, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Abbrescia
authors.affiliation: SMBNOS, University of Bari, Italy.
authors.forename: P L
authors.initials: PL
authors.lastname: Martino
authors.affiliation: SMBNOS, University of Bari, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Panelli
authors.affiliation: SMBNOS, University of Bari, Italy.
authors.forename: A M
authors.initials: AM
authors.lastname: Sardanelli
authors.affiliation: Institute of Biomembranes and Bioenergetics (IBBE), Italian Research Council (CNR), Bari, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Papa
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita' del Salento, Lecce, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Alifano
authors.affiliation: SMBNOS, University of Bari, Italy.
authors.forename: L L
authors.initials: LL
authors.lastname: Palese
authors.affiliation: Nanoscience Institute-CNR, U.O.S. NNL, Lecce, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Gaballo
keywords: Alkaliphilic bacilli; Bacillus clausii; Bioenergetics; Cyanide sensitivity; Respiratory chain; Terminal oxidases