title: A critique of methods for temperature imaging in single cells.
abstract: We argue that standard thermodynamic considerations and scaling laws show that a single cell cannot substantially raise its temperature by endogenous thermogenesis. This statement seriously questions the interpretations of recent work reporting temperature heterogeneities measured in single living cells.
authors.affiliation: Institut Fresnel, Centre National de la Recherche Scientifique (CNRS), Aix-Marseille Université, Centrale Marseille, Marseille, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Baffou
authors.affiliation: Institut Fresnel, Centre National de la Recherche Scientifique (CNRS), Aix-Marseille Université, Centrale Marseille, Marseille, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Rigneault
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Aix-Marseille Université, INSERM, CNRS, Marseille, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Marguet
authors.affiliation: cole Normale Supérieure, Paris Sciences et Lettres Research University, Sorbonne Universités, Université Pierre et Marie Curie, Université Paris 06, CNRS, Paris, France.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Jullien
mesh_terms: D000465:Algorithms; D000818:Animals; D016477:Artifacts; D002468:Cell Physiological Phenomena; D003198:Computer Simulation; D006801:Humans; D008954:Models, Biological; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013817:Thermography / Q000379:methods*