title: Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome.
abstract: In targeted proteomics it is critical that peptides are not only proteotypic but also accurately represent the level of the protein (quantotypic). Numerous approaches are used to identify proteotypic peptides, but quantotypic properties are rarely assessed. We show that measuring ratios of proteotypic peptides across biological samples can be used to empirically identify peptides with good quantotypic properties. We applied this technique to identify quantotypic peptides for 21% of the human kinome.
authors.affiliation: Division of Cancer Biology, The Institute of Cancer Research, London, UK.
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Worboys
authors.affiliation: Division of Cancer Biology, The Institute of Cancer Research, London, UK.
authors.forename: John
authors.initials: J
authors.lastname: Sinclair
authors.affiliation: Division of Molecular Pathology, The Institute of Cancer Research, London, UK.
authors.forename: Yinyin
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: 1] Division of Cancer Biology, The Institute of Cancer Research, London, UK. [2].
authors.forename: Claus
authors.initials: C
authors.lastname: Jørgensen
mesh_terms: D000465:Algorithms; D045744:Cell Line, Tumor; D002845:Chromatography / Q000379:methods; D019295:Computational Biology / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D010455:Peptides / Q000737:chemistry; D011494:Protein Kinases / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
chemical_list: D010455:Peptides; D011506:Proteins; D020543:Proteome; D011494:Protein Kinases