title: Local VEGF inhibition prevents ovarian alterations associated with ovarian hyperstimulation syndrome.
abstract: The relationship between human chorionic gonadotropin and ovarian hyperstimulation syndrome (OHSS) is partially mediated by vascular endothelial growth factor A (VEGF). The aim of this study was to investigate the effects of VEGF inhibition on the development of corpora lutea (CL) and cystic structures, steroidogenesis, apoptosis, cell proliferation, endothelial cell area, VEGF receptors (KDR and Flt-1), claudin-5 and occludin levels in ovaries from an OHSS rat model. The VEGF inhibitor used (VEGF receptor-1 (FLT-1)/Fc chimera, TRAP) decreased the concentrations of progesterone and estradiol as well as the percentage of CL and cystic structures in OHSS rats, and increased apoptosis in CL. Endothelial cell area in CL and KDR expression and its phosphorylation were increased, whereas claudin-5 and occludin levels were decreased in the OHSS compared to the control TRAP reversed these parameters. Our findings indicate that VEGF inhibition prevents the early onset of OHSS and decreases its severity in rats.
authors.affiliation: Instituto de Biología y Medicina Experimental (IByME) - CONICET, Buenos Aires, Argentina.
authors.forename: Leopoldina
authors.initials: L
authors.lastname: Scotti
authors.affiliation: Instituto de Biología y Medicina Experimental (IByME) - CONICET, Buenos Aires, Argentina.
authors.forename: Dalhia
authors.initials: D
authors.lastname: Abramovich
authors.affiliation: Instituto de Biología y Medicina Experimental (IByME) - CONICET, Buenos Aires, Argentina.
authors.forename: Natalia
authors.initials: N
authors.lastname: Pascuali
authors.affiliation: Instituto de Biología y Medicina Experimental (IByME) - CONICET, Buenos Aires, Argentina.
authors.forename: Griselda
authors.initials: G
authors.lastname: Irusta
authors.affiliation: Instituto de Biología y Medicina Experimental (IByME) - CONICET, Buenos Aires, Argentina.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Meresman
authors.affiliation: Instituto de Biología y Medicina Experimental (IByME) - CONICET, Buenos Aires, Argentina; Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Marta
authors.initials: M
authors.lastname: Tesone
authors.affiliation: Instituto de Biología y Medicina Experimental (IByME) - CONICET, Buenos Aires, Argentina. Electronic address: fparborell@gmail.com.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Parborell
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D062507:Claudin-5 / Q000378:metabolism; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D004958:Estradiol / Q000097:blood; D005260:Female; D062793:Occludin / Q000378:metabolism; D016471:Ovarian Hyperstimulation Syndrome / Q000378:metabolism* / Q000473:pathology; D010053:Ovary / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D011374:Progesterone / Q000097:blood; D017207:Rats, Sprague-Dawley; D040262:Receptors, Vascular Endothelial Growth Factor / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors*; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism
chemical_list: D020533:Angiogenesis Inhibitors; D062507:Claudin-5; C491510:Cldn5 protein, rat; D062793:Occludin; C568987:Ocln protein, rat; D011993:Recombinant Fusion Proteins; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; C533178:aflibercept; D011374:Progesterone; D004958:Estradiol; C501164:Flt1 protein, rat; D040262:Receptors, Vascular Endothelial Growth Factor; D040281:Vascular Endothelial Growth Factor Receptor-1; D040301:Vascular Endothelial Growth Factor Receptor-2
keywords: Angiogenesis; Apoptosis; Cell proliferation; OHSS; Ovary; VEGF