title: Bimolecular fluorescence complementation (BiFC) in live Drosophila embryos.
abstract: Bimolecular fluorescence complementation (BiFC) is a powerful method for studying protein-protein interactions in different cell types and organisms. This method was recently developed in the fruit fly Drosophila melanogaster, allowing analyzing protein interaction properties in a physiologically relevant developing context. Here we present a detailed protocol for performing BiFC with the Venus fluorescent protein in live Drosophila embryos, taking the Hox-PBC partnership as an illustrative test case. This protocol applies to any transcription factor and split fluorescent protein in general.
authors.affiliation: Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon - CNRS UMR5242, 46 Allée d'Italie, 69364, Lyon Cedex 07, France.
authors.forename: Marilyne
authors.initials: M
authors.lastname: Duffraisse
authors.forename: Bruno
authors.initials: B
authors.lastname: Hudry
authors.forename: Samir
authors.initials: S
authors.lastname: Merabet
mesh_terms: D000818:Animals; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000378:metabolism*; D025941:Protein Interaction Mapping / Q000379:methods*; D014158:Transcription, Genetic
chemical_list: D018398:Homeodomain Proteins