title: Preventive efficacy of a topical combination of fipronil--(S)-methoprene--eprinomectin--praziquantel against ear mite (Otodectes cynotis) infestation of cats through a natural infestation model.
abstract: A study based on naturally infested cats was designed to evaluate the effectiveness of a single treatment with a topical formulation containing fipronil, (S)-methoprene, eprinomectin and praziquantel, for the prevention of Otodectes cynotis infestation in cats. Six treated cats and six untreated cats were housed with three chronically Otodectes cynotis-infested cats, respectively. The cats of each group were kept together in a 20-m(2) room for 1 month. Both clinical examination and ear mite counts were conducted on Day 28. All donor cats were confirmed to be chronically infested with Otodectes cynotis on Day -1 and Day 28. From untreated control cats, 129 live mites were recovered on Day 28 and all cats were found to be infested. In the treated group, three cats were found to be infested, with a total of five live mites recovered, the difference between the two groups being significant (p = 0.003). One treatment corresponded to 96% preventive efficacy at Day 28 based on ear mite counts. With regard to cerumen, the clinical score increased significantly for untreated cats between Day -1 and Day 28 (p = 0.00026) and not for treated cats (p = 0.30). The difference in cerumen abundance was significant between untreated and treated cats on Day 28 (p = 0.0035). Concerning the pruritic reflex in at least one ear, all cats were negative at inclusion. All six untreated cats became positive and showed a reflex on Day 28, whereas no treated cat showed ear pruritus (p = 0.00026).
authors.affiliation: Merial, 29 avenue Tony Garnier, 69007 Lyon, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Beugnet
authors.affiliation: Parasitologie, École Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Bouhsira
authors.affiliation: Merial, 29 avenue Tony Garnier, 69007 Lyon, France.
authors.forename: Lénaïg
authors.initials: L
authors.lastname: Halos
authors.affiliation: Parasitologie, École Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Franc
mesh_terms: D056810:Acaricides / Q000008:administration & dosage / Q000627:therapeutic use*; D000287:Administration, Topical; D000818:Animals; D002371:Cat Diseases / Q000188:drug therapy / Q000469:parasitology / Q000517:prevention & control* / Q000635:transmission; D002415:Cats; D004338:Drug Combinations; D004424:Ear Canal / Q000469:parasitology; D004427:Ear Diseases / Q000188:drug therapy / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D005260:Female; D007559:Ivermectin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D008297:Male; D008726:Methoprene / Q000008:administration & dosage / Q000627:therapeutic use*; D008924:Mite Infestations / Q000188:drug therapy / Q000469:parasitology / Q000517:prevention & control / Q000635:transmission / Q000662:veterinary*; D038781:Otoscopy; D011223:Praziquantel / Q000008:administration & dosage / Q000627:therapeutic use*; D011537:Pruritus / Q000209:etiology / Q000662:veterinary; D040042:Psoroptidae; D011720:Pyrazoles / Q000008:administration & dosage / Q000627:therapeutic use*; D016037:Single-Blind Method; D007507:Therapeutic Irrigation
chemical_list: D056810:Acaricides; D004338:Drug Combinations; D011720:Pyrazoles; D011223:Praziquantel; D007559:Ivermectin; C101434:eprinomectin; D008726:Methoprene; C082360:fipronil