title: Latency of bovine herpesvirus type 5 (BoHV-5) in tonsils and peripheral blood leukocytes.
abstract: Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) can both establish latency in the trigeminal ganglion. Non-neural sites of latency have been described for BoHV-1 but not for BoHV-5. The aim of this study was to determine whether peripheral blood leukocytes and tonsils are targets for BoHV-5 infection and to establish whether all stages of that virus's infectious cycle can occur in those cell types. Comparisons with BoHV-1 infection of these tissues were also made in order to better understand the pathogenesis of both viruses. BoHV-1 and BoHV-5 were isolated from tonsils of acutely-infected calves. BoHV-5 was also isolated from a tonsil homogenate after dexamethasone-induced reactivation. During latency, infectious virus was recovered from a tonsil explant of one BoHV-5-infected calf. The genomes of BoHV-5 and BoHV-1 were detected in tonsils from acutely-infected calves although were not detected in tonsils from latently-infected calves or from calves treated with dexamethasone. Virus DNA was intermittently detected in leukocytes. The study has shown that BoHV-5 can establish latency in bovine tonsils and peripheral white blood cells, and that it can be reactivated from latently-infected tonsils, which might contribute to viral transmission. The titres of BoHV-1 and BoHV-5 in tonsils were similar, suggesting that replication at this site is a common feature for both viruses.
authors.affiliation: Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Paraje Arroyo Seco S/N, Tandil 7000, Argentina.
authors.forename: P A
authors.initials: PA
authors.lastname: Favier
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917, C1033AAJ, Buenos Aires, Argentina.
authors.forename: M S
authors.initials: MS
authors.lastname: Marin
authors.affiliation: Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Paraje Arroyo Seco S/N, Tandil 7000, Argentina.
authors.forename: P E
authors.initials: PE
authors.lastname: Morán
authors.affiliation: Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria Balcarce, Ruta 226 Km 73.5 (7620), Balcarce, Buenos Aires, Argentina.
authors.forename: A C
authors.initials: AC
authors.lastname: Odeón
authors.affiliation: Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria Balcarce, Ruta 226 Km 73.5 (7620), Balcarce, Buenos Aires, Argentina.
authors.forename: A E
authors.initials: AE
authors.lastname: Verna
authors.affiliation: Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Paraje Arroyo Seco S/N, Tandil 7000, Argentina; Centro de Investigación Veterinaria de Tandil (CIVETAN)-CONICET, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Paraje Arroyo Seco S/N, Tandil 7000, Argentina. Electronic address: seperez@vet.unicen.edu.ar.
authors.forename: S E
authors.initials: SE
authors.lastname: Pérez
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology; D018792:Encephalitis, Viral / Q000662:veterinary / Q000821:virology; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000502:physiology; D025881:Herpesvirus 5, Bovine / Q000302:isolation & purification / Q000502:physiology*; D007962:Leukocytes / Q000821:virology*; D008198:Lymph Nodes / Q000473:pathology / Q000821:virology; D008590:Meningoencephalitis / Q000662:veterinary* / Q000821:virology; D014066:Palatine Tonsil / Q000473:pathology / Q000821:virology*; D017735:Virus Latency / Q000502:physiology*
keywords: BoHV-1; BoHV-5; Latency; Peripheral blood leukocytes; Tonsils