title: A simple resonance Rayleigh scattering method for determination of trace CA125 using immuno-AuRu nanoalloy as probe via ultrasonic irradiation.
abstract: AuRu nanoalloy (GR) with Au/Ru molar ratio of 32/1 was prepared by the sodium borohydride reduction method. It was used to label the CA125 antibody (Ab) to obtain an immunonanoprobe (GRAb) for cancer antigen 125 (CA125). In pH 7.0 citric acid-Na2HPO4 buffer solution and irradiation of ultrasound, the probes were aggregated nonspecifically to big clusters that showed a strong resonance Rayleigh scattering (RRS) peak at 278 nm. Upon addition of CA125, GRAb reacted specifically with CA125 to form dispersive immunocomplexes of CA125-GRAb in the solution and this process enhanced by the ultrasonic cavitation effect, which led to the RRS intensity decreased greatly. The decreased RRS intensity was linear to the concentration of CA125 in the range of 1.3-80 U/mL, with a detection limit of 0.6 U/mL. The proposed method was applied to detect CA125 in real sample, with satisfactory results.
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin 541004, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Tang
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin 541004, China.
authors.forename: Guiqing
authors.initials: G
authors.lastname: Wen
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin 541004, China.
authors.forename: Yanghe
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin 541004, China.
authors.forename: Aihui
authors.initials: A
authors.lastname: Liang
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin 541004, China. Electronic address: zljiang@mailbox.gxnu.edu.cn.
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D065966:Absorption, Physicochemical; D000497:Alloys / Q000737:chemistry*; D002021:Buffers; D018394:CA-125 Antigen / Q000097:blood*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods*; D008565:Membrane Proteins / Q000097:blood*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D012428:Ruthenium / Q000737:chemistry*; D012542:Scattering, Radiation; D013997:Time Factors; D014465:Ultrasonics
chemical_list: D000497:Alloys; D002021:Buffers; D018394:CA-125 Antigen; C451759:MUC16 protein, human; D008565:Membrane Proteins; D006046:Gold; D012428:Ruthenium
keywords: AuRu nanoalloy; CA125; Immunoassay; Resonance Rayleigh scattering; Ultrasound