title: A comparative study of different aspects of manipulating ratio spectra applied for ternary mixtures: derivative spectrophotometry versus wavelet transform.
abstract: This work represents a comparative study of different aspects of manipulating ratio spectra, which are: double divisor ratio spectra derivative (DR-DD), area under curve of derivative ratio (DR-AUC) and its novel approach, namely area under the curve correction method (AUCCM) applied for overlapped spectra; successive derivative of ratio spectra (SDR) and continuous wavelet transform (CWT) methods. The proposed methods represent different aspects of manipulating ratio spectra of the ternary mixture of Ofloxacin (OFX), Prednisolone acetate (PA) and Tetryzoline HCl (TZH) combined in eye drops in the presence of benzalkonium chloride as a preservative. The proposed methods were checked using laboratory-prepared mixtures and were successfully applied for the analysis of pharmaceutical formulation containing the cited drugs. The proposed methods were validated according to the ICH guidelines. A comparative study was conducted between those methods regarding simplicity, limitation and sensitivity. The obtained results were statistically compared with those obtained from the reported HPLC method, showing no significant difference with respect to accuracy and precision.
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 11787 6th October City, Egypt.
authors.forename: Hesham
authors.initials: H
authors.lastname: Salem
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr-El Aini Street, 11562 Cairo, Egypt.
authors.forename: Hayam M
authors.initials: HM
authors.lastname: Lotfy
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr-El Aini Street, 11562 Cairo, Egypt.
authors.forename: Nagiba Y
authors.initials: NY
authors.lastname: Hassan
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Modern University for Technology and Information (MTI), 12582 Al Hadaba Al Wosta, Cairo, Egypt.
authors.forename: Mohamed B
authors.initials: MB
authors.lastname: El-Zeiny
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 11787 6th October City, Egypt. Electronic address: drsarahsalah@gmail.com.
authors.forename: Sarah S
authors.initials: SS
authors.lastname: Saleh
mesh_terms: D019540:Area Under Curve; D007093:Imidazoles / Q000737:chemistry*; D015242:Ofloxacin / Q000737:chemistry*; D009883:Ophthalmic Solutions / Q000032:analysis; D011239:Prednisolone / Q000031:analogs & derivatives* / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods*; D058067:Wavelet Analysis
chemical_list: D007093:Imidazoles; D009883:Ophthalmic Solutions; C009935:prednisolone acetate; D011239:Prednisolone; D015242:Ofloxacin; C005810:tetrahydrozoline
keywords: Area under the curve; Derivative of ratio spectra; Ofloxacin; Prednisolone; Tetryzoline; Wavelet transform