title: srGAP3 promotes neurite outgrowth of dorsal root ganglion neurons by inactivating RAC1.
abstract: OBJECTIVE
  To explore effect of srGAP3 promotes neurite outgrowth of dorsal root ganglion neurons.
  
  
  METHODS
  In this study, expression of Slit1 was observed predominantly in the glia, while expression of Robo2 and srGAP3 was detected in sensory neurons of postnatal rat cultured dorsal root ganglion (DRG). Furthermore, upregulation of srGAP3 following sciatic nerve transection was detected by immunohistochemistry and Western blotting.
  
  
  RESULTS
  It was observed that inhibition of neurite outgrowth in cultured adult DRG neurons following treatment with anti-srGAP3 or anti-Robo2 was more effectively (1.5-fold higher) than that following treatment with an anti-BDNF positive control antibody. It demonstrated that srGAP3 interacted with Robo2 and Slit1 protein to decrease Rac1-GTP activity in cultured adult rat DRG neurons and the opposite effect on Rac1-GTP activity was detected by co-immunoprecipitation and immunoblotting analyses following treatment with anti-Robo2 or anti-srGAP3. These data demonstrated a role for srGAP3 in neurite outgrowth of DRG sensory neurons.
  
  
  CONCLUSIONS
  Our observations suggest that srGAP3 promotes neurite outgrowth and filopodial growth cones by interacting with Robo2 to inactivate Rac1 in mammalian DRG neurons.
authors.affiliation: Department of Anatomy, Hainan Medical College, Haikou 571101, Hainan, China; Neuroscience Research Institute, Hainan Medical College, Haikou 571101, Hainan, China.
authors.forename: Quan-Peng
authors.initials: QP
authors.lastname: Zhang
authors.affiliation: Department of Anatomy, Hainan Medical College, Haikou 571101, Hainan, China; Neuroscience Research Institute, Hainan Medical College, Haikou 571101, Hainan, China.
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Zhang
authors.affiliation: Department of Anatomy, Hainan Medical College, Haikou 571101, Hainan, China.
authors.forename: Xian-Fang
authors.initials: XF
authors.lastname: Zhang
authors.affiliation: Department of Neurology, The Affiliated Hospital, Hainan Medical College, Haikou 571101, Hainan, China.
authors.forename: Jiu-Hong
authors.initials: JH
authors.lastname: Zhao
authors.affiliation: Department of Anatomy, Hainan Medical College, Haikou 571101, Hainan, China.
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Ma
authors.affiliation: Department of Anatomy, Hainan Medical College, Haikou 571101, Hainan, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Department of Anatomy, Hainan Medical College, Haikou 571101, Hainan, China; Neuroscience Research Institute, Hainan Medical College, Haikou 571101, Hainan, China. Electronic address: Yixn001@google.com.
authors.forename: Xi-Nan
authors.initials: XN
authors.lastname: Yi
mesh_terms: D000818:Animals; D020690:GTPase-Activating Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000166:cytology* / Q000293:injuries / Q000378:metabolism*; D016501:Neurites / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000502:physiology*; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism; D020830:rac1 GTP-Binding Protein / Q000378:metabolism*
chemical_list: D020690:GTPase-Activating Proteins; C530215:SRGAP3 protein, rat; C499134:Rac1 protein, rat; D020764:cdc42 GTP-Binding Protein; D020830:rac1 GTP-Binding Protein
keywords: Dorsal root ganglion; Rats; Slit-Robo GTPase activating protein 3