title: Fast determination of ethylene glycol, 1,2-propylene glycol and glycolic acid in blood serum and urine for emergency and clinical toxicology by GC-FID.
abstract: A simple, cost effective, and fast gas chromatography method with flame ionization detection (GC-FID) for simultaneous measurement of ethylene glycol, 1,2-propylene glycol and glycolic acid was developed and validated for clinical toxicology purposes. This new method employs a relatively less used class of derivatization agents - alkyl chloroformates, allowing the efficient and rapid derivatization of carboxylic acids within seconds while glycols are simultaneously derivatized by phenylboronic acid. The entire sample preparation procedure is completed within 10 min. To avoid possible interference from naturally occurring endogenous acids and quantitation errors 3-(4-chlorophenyl) propionic acid was chosen as an internal standard. The significant parameters of the derivatization have been found using chemometric procedures and these parameters were optimized using the face-centered central composite design. The calibration dependence of the method was proved to be quadratic in the range of 50-5000 mg mL(-1), with adequate accuracy (92.4-108.7%) and precision (9.4%). The method was successfully applied to quantify the selected compounds in serum of patients from emergency units.
authors.affiliation: Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic. Electronic address: tomas.hlozek@vfn.cz.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Hložek
authors.affiliation: Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic; Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic.
authors.forename: Miroslava
authors.initials: M
authors.lastname: Bursová
authors.affiliation: Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic; Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic.
authors.forename: Radomír
authors.initials: R
authors.lastname: Čabalaa
mesh_terms: D016022:Case-Control Studies; D002849:Chromatography, Gas / Q000379:methods; D004632:Emergency Medical Services; D019855:Ethylene Glycol / Q000097:blood* / Q000506:poisoning / Q000652:urine*; D005260:Female; D005410:Flame Ionization / Q000379:methods; D006016:Glycolates / Q000097:blood* / Q000506:poisoning / Q000652:urine*; D006801:Humans; D008297:Male; D019946:Propylene Glycol / Q000097:blood* / Q000506:poisoning / Q000652:urine*; D044967:Serum / Q000737:chemistry; D014116:Toxicology / Q000379:methods
chemical_list: D006016:Glycolates; C031149:glycolic acid; D019946:Propylene Glycol; D019855:Ethylene Glycol
keywords: Chloroformate; Experimental design; GC; Glycolic acid; Glycols