title: Monocyte chemoattractant protein-1 (MCP-1) g.-2518A>G polymorphism and susceptibility to type 2 diabetes (T2D) and end stage renal disease (ESRD) in the North-West Indian population of Punjab.
abstract: BACKGROUND
  Diabetic nephropathy (DN) is a major microvascular complication that develops in nearly 20-30% of patients with type 2 diabetes (T2D) and is currently the leading cause of end stage renal disease (ESRD). Monocyte chemoattractant protein-1 (MCP-1), a potent chemokine secreted by adipocytes, has been implicated as a causal factor in the progression of vascular complications in T2D, thus MCP-1 appears to be a promising candidate for association study.
  
  
  OBJECTIVE
  The objective of the present study is to evaluate the association, if any, of g.-2518A>G polymorphism (rs1024611) in MCP-1 gene in T2D cases with and without ESRD in the population of Punjab from North-West India.
  
  
  METHODS
  A total of 571 samples from Punjab comprising 350 T2D cases (145 with ESRD and 205 without ESRD) and 221 controls were genotyped for g.-2518A>G MCP-1 polymorphism using amplification refractory mutation system- polymerase chain reaction.
  
  
  RESULTS
  The frequency of G allele was observed to be higher in T2D cases with ESRD (34.49%) compared to T2D cases without ESRD (24.39%) and controls (31.67%). Under the dominant model, G allele increased the risk of ESRD by 1.68-fold [p = 0.047, OR = 1.68 (1.0-2.79) at 95% CI].
  
  
  CONCLUSIONS
  MCP-1 -2518 GG genotype and G allele may increase the risk of progression to ESRD in T2D cases.
authors.affiliation: Department of Human Genetics, Guru Nanak Dev University , Amritsar, Punjab , India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Raina
authors.forename: Kawaljit
authors.initials: K
authors.lastname: Matharoo
authors.forename: A J S
authors.initials: AJ
authors.lastname: Bhanwer
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000235:genetics* / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000453:epidemiology* / Q000235:genetics; D004198:Disease Susceptibility / Q000453:epidemiology / Q000209:etiology; D005260:Female; D006801:Humans; D007194:India / Q000453:epidemiology; D007676:Kidney Failure, Chronic / Q000453:epidemiology* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2
keywords: Association analysis; ESRD; MCP-1; T2D; survival analysis