title: Soil genotoxicity assessment--results of an interlaboratory study on the Vicia micronucleus assay in the context of ISO standardization.
abstract: The Vicia micronucleus assay was standardized in an international protocol, ISO 29200, "Assessment of genotoxic effects on higher plants-Vicia faba micronucleus test," for soil or soil materials (e.g., compost, sludge, sediment, waste, and fertilizing materials). The aim of this interlaboratory study on the Vicia micronucleus assay was to investigate the robustness of this in vivo assay in terms of its applicability in different countries where each participant were asked to use their own seeds and reference soil, in agreement with the ISO 29200 standard. The ISO 29200 standard protocol was adopted for this study, and seven laboratories from three countries (France, Italy, and Brazil) participated in the study. Negative and positive controls were correctly evaluated by 100 % of the participants. In the solid-phase test, the micronucleus frequency (number of micronuclei/1,000 cells) varied from 0.0 to 1.8 for the negative control (i.e., Hoagland's solution) and from 5.8 to 85.7 for the positive control (i.e., maleic hydrazide), while these values varied from 0.0 to 1.7 for the negative control and from 14.3 to 97.7 for the positive control in the case of liquid-phase test. The variability in the data obtained does not adversely affect the robustness of the protocol assessed, on the condition that the methodology described in the standard ISO 29200 is strictly respected. Thus, the Vicia micronucleus test (ISO 29200) is appropriate for complementing prokaryotic or in vitro tests cited in legislation related to risk assessment of genotoxicity potential.
authors.affiliation: Laboratoire Interdisciplinaire des Environnements Continentaux, Université de Lorraine (LIEC), CNRS UMR 7360, 8, Rue du General Delestraint, Metz, 57070, France, sylvie.cotelle@univ-lorraine.fr.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Cotelle
authors.forename: Adrien
authors.initials: A
authors.lastname: Dhyèvre
authors.forename: Serge
authors.initials: S
authors.lastname: Muller
authors.forename: Pascale
authors.initials: P
authors.lastname: Chenon
authors.forename: Nicolas
authors.initials: N
authors.lastname: Manier
authors.forename: Pascal
authors.initials: P
authors.lastname: Pandard
authors.forename: Abdelwahad
authors.initials: A
authors.lastname: Echairi
authors.forename: Jérôme
authors.initials: J
authors.lastname: Silvestre
authors.forename: Maritxu
authors.initials: M
authors.lastname: Guiresse
authors.forename: Eric
authors.initials: E
authors.lastname: Pinelli
authors.forename: Lucia
authors.initials: L
authors.lastname: Giorgetti
authors.forename: Meri
authors.initials: M
authors.lastname: Barbafieri
authors.forename: Valéria C
authors.initials: VC
authors.lastname: Silva
authors.forename: Fernanda
authors.initials: F
authors.lastname: Engel
authors.forename: Claudemir M
authors.initials: CM
authors.lastname: Radetski
mesh_terms: D015162:Micronucleus Tests / Q000379:methods / Q000592:standards*; D012989:Soil Pollutants / Q000032:analysis*; D031307:Vicia faba / Q000187:drug effects* / Q000235:genetics*
chemical_list: D012989:Soil Pollutants