title: Identification of SNP in HSP90AB1 and its association with the relative thermotolerance and milk production traits in Indian dairy cattle.
abstract: Heat shock proteins (Hsp) play crucial role in cellular thermotolerance and heat stress response. In the present work, Allele specific PCR (AS-PCR) was standardized to detect the nucleotide polymorphism within the HSP90AB1 gene (SNP g.4338T>C) in Indian breeds of dairy cattle. The identified genotypes were associated with relative thermotolerance in terms of physiological parameters and milk production traits. The results of the experiments revealed that the genotype frequency of CC, CT, and TT for Sahiwal were 0.05, 0.78, and 0.17, respectively, and in Frieswal, the frequencies were 0.20, 0.70, and 0.10, respectively. The average rectal temperature (ART) and average respiration rates (ARR) were recorded during peak summer stress and heat tolerance coefficient (HTC) was calculated. The association studies indicated that TT genotypes had significantly (P < 0.01) higher HTC and lower ARR values than CT and CC in both the breeds. The TT genotype animals also had better production parameter in terms of total milk yield (TMY) (P < 0.01). These findings may partly suggest the role of HSP90AB1 polymorphisms in the regulation of heat stress response and consequent effect on production traits. Nevertheless, involvement of other regulatory mechanisms cannot be overruled.
authors.affiliation: a School of Atmospheric Stress Management, National Institute of Abiotic Stress Management , Pune , MH , India.
authors.forename: Basavaraj
authors.initials: B
authors.lastname: Sajjanar
authors.forename: Rajib
authors.initials: R
authors.lastname: Deb
authors.forename: Umesh
authors.initials: U
authors.lastname: Singh
authors.forename: Sushil
authors.initials: S
authors.lastname: Kumar
authors.forename: Manoj
authors.initials: M
authors.lastname: Brahmane
authors.forename: Avinash
authors.initials: A
authors.lastname: Nirmale
authors.forename: Santanu Kumar
authors.initials: SK
authors.lastname: Bal
authors.forename: P S
authors.initials: PS
authors.lastname: Minhas
mesh_terms: D000818:Animals; D001831:Body Temperature; D002417:Cattle / Q000235:genetics / Q000502:physiology*; D005260:Female; D056726:Genetic Association Studies; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics*; D008892:Milk / Q000502:physiology*; D020641:Polymorphism, Single Nucleotide
chemical_list: D018841:HSP90 Heat-Shock Proteins
keywords: AS-PCR; Frieswal; HSP90AB1; HTC; SNP; Sahiwal