title: Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro.
abstract: Catechol groups can be involved in redox cycling accompanied by generation of reactive oxygen species (ROS) which may lead to oxidative damage of cellular macromolecules including DNA. The objective of this investigation was to evaluate possible genotoxic effects of a natural catecholamine adrenaline in cultured human lymphocytes using cytogenetic (sister chromatid exchange and micronuclei) and the single cell gel electrophoresis (Comet) assay. In cytogenetic tests, six experimental concentrations of adrenaline were used in a range from 0.01-500 μM. There were no indications of genotoxic effects of adrenaline in sister chromatid exchange and micronucleus tests. However, at four highest concentrations of adrenaline (5 μM, 50 μM, 150 μM and 300 μM) we observed a decreased mitotic index and cell-cycle delay. In addition, in the Comet assay we used adrenaline in a range from 0.0005-500 μM, at two treatment times: 15 min or 60 min. In contrast to cytogenetic analysis, there was a dose-dependent increase of DNA damage detected in the Comet assay. These effects were significantly reduced by concomitant treatment with quercetin or catalase. Therefore, the obtained results indicate that adrenaline may exhibit genotoxic effects in cultured human lymphocytes, most likely due to production of reactive oxygen species.
authors.affiliation: Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Oslobodjenja Blvd. 18, Belgrade, Serbia. Electronic address: ndjelic@sbb.rs.
authors.forename: Ninoslav
authors.initials: N
authors.lastname: Djelić
authors.affiliation: Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Oslobodjenja Blvd. 18, Belgrade, Serbia.
authors.forename: Milena
authors.initials: M
authors.lastname: Radaković
authors.affiliation: Department of Biology and Human Genetics, Institute of Physiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, Belgrade, Serbia.
authors.forename: Biljana
authors.initials: B
authors.lastname: Spremo-Potparević
authors.affiliation: Department of Biology and Human Genetics, Institute of Physiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, Belgrade, Serbia.
authors.forename: Lada
authors.initials: L
authors.lastname: Zivković
authors.affiliation: Institute for Pharmaceutical Research and Development, Galenika, Pasterova 2, 11000 Belgrade, Serbia.
authors.forename: Vladan
authors.initials: V
authors.lastname: Bajić
authors.affiliation: Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Oslobodjenja Blvd. 18, Belgrade, Serbia.
authors.forename: Jevrosima
authors.initials: J
authors.lastname: Stevanović
authors.affiliation: Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Oslobodjenja Blvd. 18, Belgrade, Serbia.
authors.forename: Zoran
authors.initials: Z
authors.lastname: Stanimirović
mesh_terms: D000328:Adult; D002374:Catalase / Q000494:pharmacology; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000037:antagonists & inhibitors / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D015162:Micronucleus Tests; D011794:Quercetin / Q000494:pharmacology; D012854:Sister Chromatid Exchange / Q000187:drug effects
chemical_list: D011794:Quercetin; D002374:Catalase; D004837:Epinephrine
keywords: DNA damage; Micronuclei; Oxidative stress; Sister chromatid exchange