title: [The relationship between NLRP3 inflammsomes expression and liver damage after allogeneic hematopoietic stem cell transplantation in mice model].
abstract: OBJECTIVE
  To explore the function of nucleotide-binding domain (NOD)-like receptor protein 3 (NLRP3) inflammsomes in liver damage after allogeneic hematopoietic stem cell transplantation (allo-HSCT).
  
  
  METHODS
  The study presented a murine (BALB/c-based) model of allo-HSCT. Chimera rate was measured by flow cytometry. The hematoxylin-eosin, Masson's trichrome, immunohistochemistry staining were used to observe the pathology changes in liver, then measured the degree of liver damage. Inflammation cells and NLRP3 were measured by Western blot, cytokines IL-1β, IL-18 and NLRP3 related genes were tested with real-time quantitative polymerase chain reaction (q-PCR).
  
  
  RESULTS
  Hematopoietic stem cells had been successfully transplanted, the chimera rate was geater than 97% on the 10th day. Liver damage occurred after allo-HSCT and suffered infiltration of inflammation cells, which reached the peak on day 15, then moved to moderate; the cytokines IL-1β, IL-18 had the similar trend with liver injury, and reached the highest level on day 15, their mRNA expressions increased by (1.19 ± 0.40) fold and (1.64 ± 0.76) fold, respectively; Meanwhile, caspase-1 had the similar trend, its mRNA expression increased by (3.51 ± 0.46) fold on day 15; the inflammasomes NLRP1, NLRP3, NLRC4 and NLRP5 expressed in liver on day 15 of post-allo-HSCT, and NLRP3 inflammasome expressed highest among them. The mRNA and protein level of NLRP3 inflammasomes were kept with the serious degree of the liver damage, its mRNA expression increased by (2.91 ± 0.41) fold on day 15.
  
  
  CONCLUSIONS
  NLRP3 inflammsome expressed in liver injury during allo-HSCT in mice, and may be one of the important factors contributed to liver injury.
authors.affiliation: Department of Hepatology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Yujin
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Hepatology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Jinyu
authors.initials: J
authors.lastname: Fu
authors.affiliation: Department of Hepatology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Jinyan
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Hepatology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Fang
authors.affiliation: Department of Hepatology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hua
authors.affiliation: Department of Hepatology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Mimi
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Hepatology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Na
authors.initials: N
authors.lastname: Guo
authors.affiliation: Department of Hepatology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Kailin
authors.initials: K
authors.lastname: Xu
authors.affiliation: Department of Hepatology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Lingyu
authors.initials: L
authors.lastname: Zeng
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D058847:Inflammasomes / Q000378:metabolism*; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D011184:Postoperative Period
chemical_list: D002352:Carrier Proteins; D058847:Inflammasomes; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human