title: Steroidal glycosides from the underground parts of Dracaena thalioides and their cytotoxic activity.
abstract: Six spirostanol glycosides (1-6) and 12 known compounds (7-18) were isolated from the underground parts of Dracaena thalioides (Agavaceae). Their structures were determined by spectroscopic analysis, including 2D NMR spectroscopic data, and chemical transformations. The isolated compounds were evaluated for cytotoxic activity against HL-60 human leukemia cells. Compounds 1, 3-6, and 8-18 showed cytotoxicity against HL-60 cells, of which 10, a bisdesmosidic spirostanol derivative, showed potent cytotoxicity against HL-60 cells with an IC50 value of 0.38μM and induced apoptosis in HL-60 cells.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16 Nanxiaojie, Dongzhimennei, Beijing 100700, China; Department of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
authors.forename: Liying
authors.initials: L
authors.lastname: Tang
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16 Nanxiaojie, Dongzhimennei, Beijing 100700, China.
authors.forename: Zhuju
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16 Nanxiaojie, Dongzhimennei, Beijing 100700, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. Electronic address: yokosuka@toyaku.ac.jp.
authors.forename: Akihito
authors.initials: A
authors.lastname: Yokosuka
authors.affiliation: Department of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Mimaki
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D031409:Dracaena / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D007564:Japan; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D013150:Spirostans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: C000594700:(25R)-17alpha-hydroxyspitost-5-en-3beta-yl O-alpha-L-rhamnopyranosyl-(1-2)-O-(alpha-L-rhamnopyranosyl-(1-3))-4-O-acetyl-beta-D-glucopyranoside; D000972:Antineoplastic Agents, Phytogenic; D006027:Glycosides; D013150:Spirostans
keywords: Agavaceae; Apoptosis; Cytotoxic activity; Dracaena thalioides; HL-60 cells; Spirostanol glycosides; Steroidal glycosides