title: Modulation of selectin-mediated adhesion of flowing lymphoma and bone marrow cells by immobilized SDF-1.
abstract: The α-chemokine, stromal-derived factor-1 (SDF-1), has been linked to the homing of circulating tumor cells to bone. SDF-1 is expressed by bone microvascular cells and osteoblasts and normally functions to attract blood-borne hematopoietic stem and progenitor cells to marrow. It has been shown that treatment of cancer cells with soluble SDF-1 results in a more aggressive phenotype; however, the relevance of the administration of the soluble protein is unclear. As such, a flow device was functionalized with P-selectin and SDF-1 to mimic the bone marrow microvasculature and the initial steps of cell adhesion. The introduction of SDF-1 onto the adhesive surface was found to significantly enhance the adhesion of lymphoma cells, as well as low-density bone marrow cells (LDBMC), both in terms of the number of adherent cells and the strength of cell adhesion. Thus, SDF-1 has a synergistic effect with P-selectin on cancer cell adhesion and may be sufficient to promote preferential metastasis to bone.
authors.affiliation: Department of Biomedical Engineering, Cornell University, 203 Weill Hall, Ithaca, NY 14853, USA. eah99@cornell.edu.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Hedges
authors.affiliation: Department of Biomedical Engineering, Cornell University, 203 Weill Hall, Ithaca, NY 14853, USA. adh83@cornell.edu.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Hughes
authors.affiliation: Division of Hematology/Oncology, Department of Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA. jane_liesveld@urmc.rochester.edu.
authors.forename: Jane L
authors.initials: JL
authors.lastname: Liesveld
authors.affiliation: Department of Biomedical Engineering, Cornell University, 203 Weill Hall, Ithaca, NY 14853, USA. mike.king@cornell.edu.
authors.forename: Michael R
authors.initials: MR
authors.lastname: King
mesh_terms: D001854:Bone Marrow Cells / Q000378:metabolism / Q000502:physiology*; D002448:Cell Adhesion; D018914:Cells, Immobilized / Q000378:metabolism / Q000502:physiology; D054377:Chemokine CXCL12 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D044085:Microfluidics; D055806:Microvessels / Q000166:cytology / Q000502:physiology; D019007:P-Selectin / Q000235:genetics / Q000378:metabolism*; D020298:U937 Cells
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019007:P-Selectin