title: A systematic approach to identify novel cancer drug targets using machine learning, inhibitor design and high-throughput screening.
abstract: We present an integrated approach that predicts and validates novel anti-cancer drug targets. We first built a classifier that integrates a variety of genomic and systematic datasets to prioritize drug targets specific for breast, pancreatic and ovarian cancer. We then devised strategies to inhibit these anti-cancer drug targets and selected a set of targets that are amenable to inhibition by small molecules, antibodies and synthetic peptides. We validated the predicted drug targets by showing strong anti-proliferative effects of both synthetic peptide and small molecule inhibitors against our predicted targets.
authors.affiliation: Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1 Canada.
authors.forename: Jouhyun
authors.initials: J
authors.lastname: Jeon
authors.affiliation: Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1 Canada.
authors.forename: Satra
authors.initials: S
authors.lastname: Nim
authors.affiliation: Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1 Canada.
authors.forename: Joan
authors.initials: J
authors.lastname: Teyra
authors.affiliation: Center for Systems Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, Toronto, ON M5S 3E1 Canada ; Department of Agricultural, Food and Environmental Sciences, University of Perugia, Perugia, 06100 Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Datti
authors.affiliation: Center for Systems Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, Toronto, ON M5S 3E1 Canada.
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Wrana
authors.affiliation: Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1 Canada ; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 3E1 Canada.
authors.forename: Sachdev S
authors.initials: SS
authors.lastname: Sidhu
authors.affiliation: Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1 Canada ; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 3E1 Canada.
authors.forename: Jason
authors.initials: J
authors.lastname: Moffat
authors.affiliation: Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1 Canada ; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 3E1 Canada ; Department of Computer Science, University of Toronto, Toronto, ON M5S 3E1 Canada.
authors.forename: Philip M
authors.initials: PM
authors.lastname: Kim