title: In vitro extracorporeal shock wave treatment enhances stemness and preserves multipotency of rat and human adipose-derived stem cells.
abstract: OBJECTIVE
  Adipose-derived progenitor/stem cells (ASCs) are discussed as a promising candidate for various tissue engineering approaches. However, its applicability for the clinic is still difficult due to intra- and inter-donor heterogeneity and limited life span in vitro, influencing differentiation capacity as a consequence to decreased multipotency.
  
  
  METHODS
  Extracorporeal shock wave treatment has been proven to be a suitable clinical tool to improve regeneration of a variety of tissues for several decades, whereas the mechanisms underlying these beneficial effects remain widely unknown.
  
  
  RESULTS
  In this study we show that human and rat adipose derived stem cells respond strongly to repetitive shock wave treatment in vitro, resulting not only in maintenance and significant elevation of mesenchymal markers (CD73, CD90, CD105), but also in significantly increased differentiation capacity towards the osteogenic and adipogenic lineage as well as toward Schwann-cell like cells even after extended time in vitro, preserving multipotency of ASCs.
  
  
  CONCLUSIONS
  ESWT might be a promising tool to improve ASC quality for cell therapy in various tissue engineering and regenerative medicine applications.
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria; University of Applied Sciences Technikum Wien-Department of Biochemical Engineering, Vienna, Austria. Electronic address: Christina.schuh@trauma.lbg.ac.at.
authors.forename: Christina Maria Anna Pia
authors.initials: CM
authors.lastname: Schuh
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: Philipp
authors.initials: P
authors.lastname: Heher
authors.affiliation: Austrian Cluster for Tissue Regeneration, Vienna, Austria; University of Applied Sciences Technikum Wien-Department of Biochemical Engineering, Vienna, Austria.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Weihs
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: Asmita
authors.initials: A
authors.lastname: Banerjee
authors.affiliation: Austrian Cluster for Tissue Regeneration, Vienna, Austria; University of Applied Sciences Technikum Wien-Department of Biochemical Engineering, Vienna, Austria.
authors.forename: Christiane
authors.initials: C
authors.lastname: Fuchs
authors.affiliation: Red Cross Blood Transfusion Service for Upper Austria, Linz, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Gabriel
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: Susanne
authors.initials: S
authors.lastname: Wolbank
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: Rainer
authors.initials: R
authors.lastname: Mittermayr
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: Heinz
authors.initials: H
authors.lastname: Redl
authors.affiliation: Austrian Cluster for Tissue Regeneration, Vienna, Austria; University of Applied Sciences Technikum Wien-Department of Biochemical Engineering, Vienna, Austria.
authors.forename: Dominik
authors.initials: D
authors.lastname: Rünzler
authors.affiliation: Austrian Cluster for Tissue Regeneration, Vienna, Austria; University of Applied Sciences Technikum Wien-Department of Biochemical Engineering, Vienna, Austria.
authors.forename: Andreas Herbert
authors.initials: AH
authors.lastname: Teuschl
mesh_terms: D000328:Adult; D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis*; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation; D019220:High-Energy Shock Waves; D006801:Humans; D008297:Male; D039902:Multipotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000943:Antigens, Differentiation
keywords: Schwann-like cells; adipose-derived stem cells; extracorporeal shock wave treatment; multipotency; stemness