title: Anti-biofilm activity of biogenic selenium nanoparticles and selenium dioxide against clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa, and Proteus mirabilis.
abstract: The aim of the present study was to investigate the anti-biofilm activity of biologically synthesized selenium nanoparticles (Se NPs) against the biofilm produced by clinically isolated bacterial strains compared to that of selenium dioxide. Thirty strains of Staphylococcus aureus, Pseudomonas aeruginosa, and Proteus mirabilis were isolated from various specimens of the patients hospitalized in different hospitals (Kerman, Iran). Quantification of the biofilm using microtiter plate assay method introduced 30% of S. aureus, 13% of P. aeruginosa and 17% of P. mirabilis isolates as severely adherent strains. Transmission electron micrograph (TEM) of the purified Se NPs (produced by Bacillus sp. MSh-1) showed individual and spherical nano-structure in the size range of 80-220nm. Obtained results of the biofilm formation revealed that selenium nanoparticles inhibited the biofilm of S. aureus, P. aeruginosa, and P. mirabilis by 42%, 34.3%, and 53.4%, respectively, compared to that of the non-treated samples. Effect of temperature and pH on the biofilm formation in the presence of Se NPs and SeO2 was also evaluated.
authors.affiliation: Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Shakibaie
authors.affiliation: Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Forootanfar
authors.affiliation: Department of Microbiology and Immunology, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Yaser
authors.initials: Y
authors.lastname: Golkari
authors.affiliation: Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Tayebe
authors.initials: T
authors.lastname: Mohammadi-Khorsand
authors.affiliation: Department of Microbiology and Immunology, Kerman University of Medical Sciences, Kerman, Iran; Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran; Environmental Health Engineering Research Centers, Kerman University of Medical Sciences, Kerman, Iran. Electronic address: mohammadreza.shakibaie@gmail.com.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Shakibaie
mesh_terms: D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011513:Proteus mirabilis / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D012643:Selenium / Q000494:pharmacology*; D064588:Selenium Oxides / Q000494:pharmacology*; D013052:Spectrometry, X-Ray Emission; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D013696:Temperature
chemical_list: D064588:Selenium Oxides; D012643:Selenium
keywords: Biofilm; Biosynthesis; Selenium dioxide; Selenium nanoparticle