title: In vitro effects of oil's fatty acids on T cell function in gestational diabetic pregnant women and their newborns.
abstract: BACKGROUND
  The aim of this investigation was to determine the in vitro effects of linseed, olive and Nigel oils on T cell proliferation and function in gestational diabetes.
  
  
  METHODS
  Blood samples were collected from 40 control healthy and 32 gestational diabetic mothers and their newborns. Peripheral blood lymphocytes were isolated using a density gradient of Ficoll. T cell proliferation, interleukin-2 and -4 (IL-2, IL-4) secretion, fatty acid composition and intracellular oxidative status were investigated.
  
  
  RESULTS
  Mitogen (Concanavalin A) stimulated lymphocyte proliferation, IL-2 secretion, intracellular reduced glutathione levels, superoxide dismutase (SOD) and catalase activities were lower while intracellular malondialdehyde (MDA) and carbonyl proteins were higher in diabetic mothers and in their newborns as compared to their respective controls. Linseed oil induced a reduction in T-lymphocyte proliferation and IL-2 production, and alpha linolenic acid membrane enrichment in both diabetic and control groups. In the presence of Nigel oil, T-lymphocyte proliferation and IL-2 secretion, phospholipid linoleic and oleic acids were enhanced. Olive oil had no effect on lymphocyte proliferation in all groups. Linseed, olive and Nigel oils induced an increase in T cell levels of reduced glutathione levels and in activities of catalase and SOD with a concomitant decrease in MDA and carbonyl protein contents.
  
  
  CONCLUSIONS
  Linseed, olive and Nigel oils had beneficial effects on T cell functions in gestational diabetes.
authors.affiliation: Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, University Abou-Bekr Belkaïd, Tlemcen, Algeria.
authors.forename: Farah
authors.initials: F
authors.lastname: Djelti
authors.affiliation: Laboratory of Physiology, Physiopathology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, University Abou-Bekr Belkaïd, Tlemcen, Algeria.
authors.forename: Hafida
authors.initials: H
authors.lastname: Merzouk
authors.affiliation: Department of Technical Sciences, Faculty of Engineering, University Abou-Bekr Belkaïd, Tlemcen, Algeria.
authors.forename: Sid Ahmed
authors.initials: SA
authors.lastname: Merzouk
authors.affiliation: INSERM UMR 866, "Lipids Nutrition Cancer", University of Burgundy, Faculty of Life, Earth and Environment Sciences, Dijon, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Narce
mesh_terms: D000328:Adult; D016640:Diabetes, Gestational / Q000276:immunology*; D005227:Fatty Acids / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn; D010938:Plant Oils / Q000008:administration & dosage*; D011247:Pregnancy; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D005227:Fatty Acids; D010938:Plant Oils
keywords: Nigel oil; gestational diabetes; linseed oil; lymphocytes; olive oil; oxidant/antioxidant status; 关键词：妊娠期糖尿病，亚麻油，淋巴细胞，奈吉尔油，橄榄油，氧化/抗氧化状态