title: Use of sulfur-oxidizing bacteria as recognition elements in hydrogen sulfide biosensing system.
abstract: Four sulfur-oxidizing bacteria (Thiobacillus thioparus, Acidithiobacillus thiooxidans PTCC1717, Acidithiobacillus ferrooxidans PTCC1646, and Acidithiobacillus ferrooxidans PTCC1647) were used as biorecognition elements in a hydrogen sulfide biosensing system. All the experiments were performed in 0.1 M phosphate buffer solution containing 1-20 ppm H2S with optimum pH and temperature for each species. Although H2 S was applied to the biosensing system, the dissolved O2 content decreased. Dissolved O2 consumed by cells in both free and immobilized forms was measured using a dissolved oxygen sensor. Free bacterial cells exhibit fast response (<200 Sec). Immobilization of the cells on polyvinyl alcohol was optimized using an analytical software. Immobilized A. ferrooxidans and A. thiooxidans retained more than 50% of activity after 30 days of immobilization. According to the data, A. thiooxidans and A. ferrooxidans are appropriate species for hydrogen sulfide biosensor.
authors.affiliation: Department of Life Science Engineering, Faculty of New Science and Technologies, University of Tehran, Tehran, Iran.|Department of Research Center in Life Science Engineering, University of Tehran, Tehran, Iran.
authors.forename: Behdokht
authors.initials: B
authors.lastname: Janfada
authors.affiliation: Department of Life Science Engineering, Faculty of New Science and Technologies, University of Tehran, Tehran, Iran.|Department of Research Center in Life Science Engineering, University of Tehran, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Yazdian
authors.affiliation: Department of Research Center in Life Science Engineering, University of Tehran, Tehran, Iran.|Department of Chemical Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran.
authors.forename: Ghassem
authors.initials: G
authors.lastname: Amoabediny
authors.affiliation: Department of Life Science Engineering, Faculty of New Science and Technologies, University of Tehran, Tehran, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Rahaie
mesh_terms: D042763:Acidithiobacillus / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D018914:Cells, Immobilized / Q000378:metabolism*; D006862:Hydrogen Sulfide / Q000032:analysis* / Q000378:metabolism; D010084:Oxidation-Reduction
chemical_list: D006862:Hydrogen Sulfide
keywords: biosensing system; biosensors; dissolved oxygen; hydrogen sulfide; immobilization; microbial communication; polyvinyl alcohol; sulfur oxidizing bacteria