title: Gelsolin: role of a functional protein in mitigating radiation injury.
abstract: The present study was conducted to explore the protective effect of exogenous gelsolin (GSN) in mice exposed to high-dose of radiation. Changes in the levels of GSNs in peripheral blood of mice and cytoplasm of cultured human intestinal epithelial cells (HIECs) were analyzed after their exposure to different doses of (137)Cs γ-rays at a fixed dose rate. The coagulation associated indices, such as prothrombin time (PT) and activated partial thromboplastin time (APTT) were measured. Effect on radiation-mediated oxidative damage was evaluated by estimating the altered glutathione (GSH) and malondialdehyde (MDA) concentrations in the blood. The results showed that radiation induced a pronounced decrease in the pGSN blood levels. However, the cGSN levels of irradiated HIECs were increased in a dose-dependent manner. Administration of recombinant human pGSN to irradiated mice resulted in an ameliorated clotting time as indicated by the PT and the APTT indices. The treatment of mice with hpGSN enhanced the blood levels of GSH while MDA concentrations were decreased indicating an improved antioxidant status. These results suggest that GSNs might play a regulatory role in the suppression of the tissue damage induced by acute radiation exposure.
authors.affiliation: Division of Radiation Medicine Department of Naval Medicine, Second Military Medical University, Shanghai, China.
authors.forename: Mingjuan
authors.initials: M
authors.lastname: Li
authors.forename: Fengmei
authors.initials: F
authors.lastname: Cui
authors.forename: Ying
authors.initials: Y
authors.lastname: Cheng
authors.forename: Ling
authors.initials: L
authors.lastname: Han
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.forename: Ding
authors.initials: D
authors.lastname: Sun
authors.forename: Yu-long
authors.initials: YL
authors.lastname: Liu
authors.forename: Ping-kun
authors.initials: PK
authors.lastname: Zhou
authors.forename: Rui
authors.initials: R
authors.lastname: Min
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects / Q000528:radiation effects; D002374:Catalase / Q000097:blood; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D005720:Gamma Rays / Q000009:adverse effects; D018260:Gelsolin / Q000097:blood / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D005978:Glutathione / Q000097:blood; D006801:Humans; D007422:Intestines / Q000166:cytology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D010314:Partial Thromboplastin Time; D011517:Prothrombin Time; D011832:Radiation Injuries / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D018260:Gelsolin; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione