title: Determination of thermodynamic and kinetic properties of biomolecules by mass spectrometry.
abstract: Over the past two decades, mass spectrometry (MS) has transformed the life sciences. The advances in understanding biomolecule structure and function by MS is progressing at an accelerated pace. MS has also largely been applied to study thermodynamic and kinetic structure of biomolecules. Herein, we highlight the recent discussions about native mass spectrometry and studies about determining stable gas phase structures, hydrogen/deuterium exchange studies about reaction kinetics and determination of binding constants of biomolecules with their ligands.
authors.affiliation: Department of Chemistry and Applied Biosciences, ETH Zurich, CH-8093 Zurich, Switzerland; Institute of Child Health, Division of Pediatric Basic Sciences, Hacettepe University, 06100 Ankara, Turkey.
authors.forename: Basri
authors.initials: B
authors.lastname: Gülbakan
authors.affiliation: Department of Chemistry and Applied Biosciences, ETH Zurich, CH-8093 Zurich, Switzerland.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Barylyuk
authors.affiliation: Department of Chemistry and Applied Biosciences, ETH Zurich, CH-8093 Zurich, Switzerland. Electronic address: zenobi@org.chem.ethz.ch.
authors.forename: Renato
authors.initials: R
authors.lastname: Zenobi
mesh_terms: D003903:Deuterium / Q000737:chemistry; D005740:Gases / Q000737:chemistry; D006801:Humans; D006859:Hydrogen / Q000737:chemistry; D007700:Kinetics; D008024:Ligands; D013058:Mass Spectrometry / Q000379:methods*; D013816:Thermodynamics
chemical_list: D005740:Gases; D008024:Ligands; D006859:Hydrogen; D003903:Deuterium