title: Facile synthesis and characterization of novel biodegradable amphiphilic block copolymers bearing pendant hydroxyl groups.
abstract: Novel amphiphilic block copolymers bearing pendant hydroxyl groups polylactide-b-poly(3,3-bis(Hydroxymethyl-triazolylmethyl) oxetane)-b-polylactide (PLA-b-PHMTYO-b-PLA) were synthesized via a facile and efficient method. First, the block copolymer intermediates polylactide-b-poly(3,3-Diazidomethyloxetane)-b-polylactide (PLA-b-PBAMO-b-PLA) were synthesized through ring-opening polymerization of lactide using PBAMO as a macroinitiator. Following "Click" reaction of PLA-b-PBAMO-b-PLA with propargyl alcohol provided the targeted amphiphilic block copolymers PLA-b-PHMTYO-b-PLA with pendant hydroxyl groups. The composition and structure of prepared copolymers were characterized by (1)H nuclear magnetic resonance ((1)H NMR) spectroscopy, Fourier transform infrared (FT-IR) and gel permeation chromatography (GPC). The self-assembly behavior of the copolymers in water was investigated by transmission electron microscope (TEM), dynamic light scattering (DLS) and static light scattering (SLS). The results showed that the novel copolymers PLA-b-PHMTYO-b-PLA self-assembled into spherical micelles with diameters ranging from 100 nm to 200 nm in aqueous solution. These copolymers also exhibited low critical micellar concentrations (CMC: 6.9 × 10(-4)mg/mL and 3.9 × 10(-5)mg/mL, respectively). In addition, the in vitro cytotoxicity of these copolymers was determined in the presence of L929 cells. The results showed that the block copolymers PLA-b-PHMTYO-b-PLA exhibited better biocompatibility. Therefore, these well-defined copolymers are expected to find some applications in drug delivery or tissue engineering.
authors.affiliation: Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, PR China.
authors.forename: Gaicen
authors.initials: G
authors.lastname: Hu
authors.affiliation: Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, PR China.
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Fan
authors.affiliation: Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, PR China.
authors.forename: Bingcan
authors.initials: B
authors.lastname: Xu
authors.affiliation: Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, PR China.
authors.forename: Delong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, PR China. Electronic address: zghu@htu.cn.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002460:Cell Line; D002850:Chromatography, Gel; D051379:Mice; D008823:Micelles; D046529:Microscopy, Electron, Transmission; D011108:Polymers / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001672:Biocompatible Materials; D008823:Micelles; D011108:Polymers
keywords: Amphiphilic block copolymer; Micelle; Self-assembly; “Click” reaction