title: Complete genomic sequence of the Vibrio alginolyticus lytic bacteriophage PVA1.
abstract: A novel Vibrio alginolyticus lytic bacteriophage was isolated from sewage samples obtained from a local aquatic market. Morphological analysis revealed that the phage, designated as PVA1, belonged to the family Podoviridae. The complete genomic sequence of phage PVA1 contained 41,529 bp with a G + C content of 43.7 % and 75 putative open reading frames. The genome was grouped into four modules, including phage structure, DNA packaging, DNA replication and regulation, and some additional functions. Further genomic comparison of the phage PVA1 with other known phages showed no significant similarities. Genes related to virulence and lysogeny were not detected in the phage genome. Our results suggest that phage PVA1 may be classified as a new Vibrio phage. We believe that these phage genomic sequence data will provide useful basic information for further molecular research on this Vibrio phage and its host as well for determining its infection/interaction mechanisms.
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China.
authors.forename: Jiancheng
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhenhui
authors.initials: Z
authors.lastname: Cao
authors.forename: Yongping
authors.initials: Y
authors.lastname: Xu
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.forename: Huaqiang
authors.initials: H
authors.lastname: Li
authors.forename: Feifei
authors.initials: F
authors.lastname: Wu
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Cao
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.forename: Shuying
authors.initials: S
authors.lastname: Li
authors.forename: Liji
authors.initials: L
authors.lastname: Jin
mesh_terms: D001435:Bacteriophages / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D001482:Base Composition; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D023061:Gene Order; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D017902:Podoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012722:Sewage / Q000821:virology*; D044149:Vibrio alginolyticus / Q000821:virology*
chemical_list: D004279:DNA, Viral; D012722:Sewage