title: Electrode-less measurement of cell layers impedance.
abstract: This paper is devoted to yet unpublished electrode-less methods (ELM) of cell layers impedance measurement based on transformer principle. The main advantage of ELM is elimination uncertainties caused by interface between electrodes and measured electrolyte. The method of avoiding distortion caused by non-ideal transformer transfer function ("deconvolution") and errors caused by residual voltage is described. The modification of original transformer based method allowing to measure an impedance of inserted object is proposed. Results of several calibration measurements confirming the proper function of ELM including example of transepithelial resistance of cells layer are presented. Crucial parts of measuring system and recommendation for their realization are included.
authors.affiliation: Institute of Physiology Academy of Sciences of the Czech Republic, Prague, Czech Republic, Faculty of Electrical Engineering Czech Technical University, Prague, Czech Republic. dado@fel.cvut.cz.
authors.forename: J
authors.initials: J
authors.lastname: Krůšek
authors.forename: S
authors.initials: S
authors.lastname: Ďaďo
mesh_terms: D000818:Animals; D002468:Cell Physiological Phenomena; D017097:Electric Impedance; D055724:Electrophysiological Phenomena / Q000502:physiology*; D004594:Electrophysiology / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D012984:Software