title: Analysis of cell surface alterations in Legionella pneumophila cells treated with human apolipoprotein E.
abstract: Binding of human apolipoprotein E (apoE) to Legionella pneumophila lipopolysaccharide was analysed at the molecular level by Fourier-transform infrared spectroscopy, thereby providing biophysical evidence for apoE-L. pneumophila lipopolysaccharide interaction. Atomic force microscopy imaging of apoE-exposed L. pneumophila cells revealed alterations in the bacterial cell surface topography and nanomechanical properties in comparison with control bacteria. The changes induced by apoE binding to lipopolysaccharide on the surface of L. pneumophila cells may participate in: (1) impeding the penetration of host cells by the bacteria; (2) suppression of pathogen intracellular growth and eventually; and (3) inhibition of the development of infection.
authors.affiliation: Department of Genetics and Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Sklodowska University, Lublin, Poland marta.szysz@poczta.umcs.lublin.pl.
authors.forename: Marta
authors.initials: M
authors.lastname: Palusinska-Szysz
authors.affiliation: Department of Immunobiology, Institute of Biology and Biochemistry, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Zdybicka-Barabas
authors.affiliation: Department of Immunobiology, Institute of Biology and Biochemistry, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Cytryńska
authors.affiliation: Department of Genetics and Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Wdowiak-Wróbel
authors.affiliation: Department of Genetics and Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Chmiel
authors.affiliation: Department of Biophysics, Institute of Physics, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Wiesław I
authors.initials: WI
authors.lastname: Gruszecki
mesh_terms: D001057:Apolipoproteins E / Q000378:metabolism*; D006801:Humans; D016952:Legionella pneumophila / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000378:metabolism; D018625:Microscopy, Atomic Force; D011485:Protein Binding; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties / Q000187:drug effects*
chemical_list: D001057:Apolipoproteins E; D008070:Lipopolysaccharides
keywords: Fourier-transform infrared spectroscopy; Legionella pneumophila; apolipoprotein E; atomic force microscopy; endotoxin