title: Chemical and cellular antioxidative properties of threadfin bream (Nemipterus spp.) surimi byproduct hydrolysates fractionated by ultrafiltration.
abstract: Protein hydrolysate from frame, bone and skin (FBSH) of threadfin bream was prepared using Virgibacillus sp. SK33 proteinase and fractionated using sequential ultrafiltration membranes with molecular weight cut-offs (MWCO) of 30, 5 and 1 kDa, respectively. Four fractions, namely FBSH-I (>30 kDa), FBSH-II (5-30 kDa), FBSH-III (1-5 kDa), and FBSH-IV (<1 kDa), were obtained. All fractions were rich in Lys, Glu/Gln, Gly, Pro, Ala, Asp/Asn, and Arg. FBSH-III and FBSH-IV showed the highest surface hydrophobicity measured by 8-anilino-1-naphthalenesulfonic acid (ANS) probe (p<0.05). FBSH-III showed the highest antioxidant activity and cytoprotective effects against tert-butyl hydroperoxide (TBHP)-induced cytotoxicity of Caco-2 cells. In addition, FBSH-III inhibited lactate dehydrogenase (LDH) leakage and intracellular reactive species (ROS) production in a dose-dependent manner. FBSH-III retained antioxidant activity and cytoprotective capacity after in vitro simulated gastrointestinal digestion. These results suggested that FBSH-III might potentially be nutraceutical peptides with antioxidative properties.
authors.affiliation: School of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
authors.forename: Chompoonuch
authors.initials: C
authors.lastname: Wiriyaphan
authors.affiliation: Department of Food Science, University of Massachusetts Amherst, USA.
authors.forename: Hang
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Department of Food Science, University of Massachusetts Amherst, USA.
authors.forename: Eric A
authors.initials: EA
authors.lastname: Decker
authors.affiliation: School of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand. Electronic address: Jirawat@sut.ac.th.
authors.forename: Jirawat
authors.initials: J
authors.lastname: Yongsawatdigul
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D010450:Endopeptidases; D005399:Fishes; D006801:Humans; D010473:Perciformes / Q000378:metabolism / Q000469:parasitology*; D011492:Protein Hydrolysates / Q000737:chemistry*; D017382:Reactive Oxygen Species; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D000975:Antioxidants; D011492:Protein Hydrolysates; D017382:Reactive Oxygen Species; D010450:Endopeptidases
keywords: Antioxidant activities; Cytoprotective effect; Protein hydrolysate; Threadfin bream; Ultrafiltration