title: Fluorescent protein-based molecular beacons by zinc finger protein-guided assembly.
abstract: Molecular beacons (MBs) are stem-loop structured oligonucleotides capable of sensitive and specific nucleic acid detection. However, large-scale usage of MBs for high throughput applications has been hindered by the many expensive and tedious chemical modifications. In this paper, we reported a new class of fluorescent protein-based molecular beacons (FP-MBs) based on zinc finger protein-guided assembly. The design consisted of a single oligonucleotide which forms a hairpin structure with an extending arm on both ends for the attachment of two different fluorescent proteins upon simple mixing. This new design allows for simplified MB assembly and modifications for a wide range of applications.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, Delaware, 19716.
authors.forename: Daniel
authors.initials: D
authors.lastname: Blackstock
authors.forename: Qing
authors.initials: Q
authors.lastname: Sun
authors.forename: Wilfred
authors.initials: W
authors.lastname: Chen
mesh_terms: D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012680:Sensitivity and Specificity; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D015335:Molecular Probes; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins
keywords: detection; in vivo; mRNA