title: Monitoring the progression of loop-mediated isothermal amplification using conductivity.
abstract: Loop-mediated isothermal amplification (LAMP) yields a large amount of DNA, as well as magnesium pyrophosphate precipitate, causing a decrease in ionic strength that can be measured with a conductivity meter. There is a clear relationship between the conductivity of the LAMP mixture solution and the duration of biochemical reaction. Moreover, there is also a clear relationship between the change in conductivity and the amount of initial template DNA over the range of 0.08 to 3.2 ng. These results demonstrate the feasibility not only for detecting the LAMP product qualitatively but also for real-time monitoring the biochemical reaction progression quantitatively using conductivity measurements.
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao 266071, People's Republic of China. Electronic address: zhangxz@ysfri.ac.cn.
authors.forename: Xuzhi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Chemistry and ChemicalEngineering, Ocean University of China, Qingdao 266100, People's Republic of China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Liu
authors.affiliation: School of Chemistry and The Australian Centre for NanoMedicine, University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: Xun
authors.initials: X
authors.lastname: Lu
authors.affiliation: School of Chemistry and The Australian Centre for NanoMedicine, University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Justin Gooding
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao 266071, People's Republic of China.
authors.forename: Qiufen
authors.initials: Q
authors.lastname: Li
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao 266071, People's Republic of China.
authors.forename: Keming
authors.initials: K
authors.lastname: Qu
mesh_terms: D004247:DNA / Q000032:analysis*; D004563:Electrochemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
chemical_list: D004247:DNA
keywords: Conductivity; Gene analysis method; Loop-mediated isothermal amplification; Monitor biochemical reaction