title: The role of p300 in the tumor progression of oral squamous cell carcinoma.
abstract: BACKGROUND
  EP300 gene encoding p300 is a candidate tumor suppressor gene. This study investigated p300 expression and gene alteration in oral squamous cell carcinoma (OSCC) specimens to assess its role in OSCC development.
  
  
  METHODS
  Genomic DNA extracted from 13 human OSCC cell lines and 40 OSCC patient specimens was subjected to methylation-specific PCR and exon sequencing. Immunohistochemical staining with primary antibodies against p300 and p53 was performed in 48 patients with OSCC. We analyzed the association between the data and clinicopathological factors of OSCC patients.
  
  
  RESULTS
  Methylation-specific PCR revealed that the EP300 promoter region was not hypermethylated in OSCC. Only one cell line demonstrated a point mutation at exon 31. On immunohistochemical examination, patients with metastatic lymph nodes (P = 0.009) and advanced clinical stage (P = 0.046) tended to show increased expression of p300. There was no statistically significant relationship between p300 expression and p53 accumulation in OSCC tissue samples. Patient survival was not correlated with p300 expression.
  
  
  CONCLUSIONS
  EP300 is not a tumor suppressor gene because there was neither epigenetic inactivation of the gene nor a mutation resulting in functional impairment. Based on p300 overexpression and its association with clinical factors in patients with OSCC, it is likely that p300 itself or one of its target genes plays a key role in the aggressive phenotypes of OSCC.
authors.affiliation: Department of Oral and Maxillofacial Pathology, School of Dentistry and Research Center for Tooth and Periodontal Regeneration (MRC), Kyung Hee University, Seoul, Korea.
authors.forename: Young-Ah
authors.initials: YA
authors.lastname: Cho
authors.forename: Ji-Soo
authors.initials: JS
authors.lastname: Hong
authors.forename: Eun-Jin
authors.initials: EJ
authors.lastname: Choe
authors.forename: Hye-Jung
authors.initials: HJ
authors.lastname: Yoon
authors.forename: Seong-Doo
authors.initials: SD
authors.lastname: Hong
authors.forename: Jae-Il
authors.initials: JI
authors.lastname: Lee
authors.forename: Sam-Pyo
authors.initials: SP
authors.lastname: Hong
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology / Q000556:secondary; D045744:Cell Line, Tumor; D003062:Codon / Q000235:genetics; D018450:Disease Progression; D050881:E1A-Associated p300 Protein / Q000235:genetics*; D004848:Epithelium / Q000473:pathology; D005091:Exons / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D016147:Genes, Tumor Suppressor; D006801:Humans; D008207:Lymphatic Metastasis / Q000473:pathology; D008297:Male; D008745:Methylation; D008875:Middle Aged; D009061:Mouth Mucosa / Q000473:pathology; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D009367:Neoplasm Staging; D017354:Point Mutation / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D017423:Sequence Analysis, RNA; D016019:Survival Analysis; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: D003062:Codon; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D050881:E1A-Associated p300 Protein; C494360:EP300 protein, human
keywords: oncogene; oral squamous cell carcinoma; p300; p53; tumor suppressor gene