title: PKCδ is required for porcine reproductive and respiratory syndrome virus replication.
abstract: Protein kinase C (PKC) that transduces signals to modulate a wide range of cellular functions has been shown to regulate a number of viral infections. Herein, we showed that inhibition of PKC with the PKC inhibitor GF109203X significantly impaired porcine reproductive and respiratory syndrome virus (PRRSV) replication. Inhibition of PKC led to virus yield reduction, which was associated with decreased viral RNA synthesis and lowered virus protein expression. And this inhibitory effect by PKC inhibitor was shown to occur at the early stage of PRRSV infection. Subsequently, we found that PRRSV infection activated PKCδ in PAMs and knockdown of PKCδ by small interfering RNA (siRNA) suppressed PRRSV replication, suggesting that novel PKCδ may play an important factor in PRRSV replication. Taken together, these data imply that PKC is involved in PRRSV infection and beneficial to PRRSV replication, extending our understanding of PRRSV replication.
authors.affiliation: State Key Laboratories of Agrobiotechnology, China; Department of Microbiology and Immunology, College of Biological Science, China Agricultural University, Beijing 100193, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: State Key Laboratories of Agrobiotechnology, China; Department of Microbiology and Immunology, College of Biological Science, China Agricultural University, Beijing 100193, China.
authors.forename: Xue-Kun
authors.initials: XK
authors.lastname: Guo
authors.affiliation: State Key Laboratories of Agrobiotechnology, China; Department of Microbiology and Immunology, College of Biological Science, China Agricultural University, Beijing 100193, China.
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Bi
authors.affiliation: College of Biological Science, Hunan Normal University, Changsha 410006, China.
authors.forename: Yihui
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: State Key Laboratories of Agrobiotechnology, China; Department of Microbiology and Immunology, College of Biological Science, China Agricultural University, Beijing 100193, China. Electronic address: whfeng@cau.edu.cn.
authors.forename: Wen-Hai
authors.initials: WH
authors.lastname: Feng
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015971:Gene Expression Regulation, Enzymologic; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D007211:Indoles / Q000494:pharmacology; D016676:Macrophages, Alveolar / Q000821:virology; D008301:Maleimides / Q000494:pharmacology; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology*; D051745:Protein Kinase C-delta / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D013552:Swine; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication / Q000502:physiology*
chemical_list: D007211:Indoles; D008301:Maleimides; D012367:RNA, Viral; D014764:Viral Proteins; D051745:Protein Kinase C-delta; C070515:bisindolylmaleimide I
keywords: PKCδ; PRRSV; Protein Kinase C inhibitor; Protein kinase C; siRNA