title: Seminal plasma affects sperm sex sorting in boars.
abstract: Two experiments were conducted in boar semen samples to evaluate how both holding time (24h) and the presence of seminal plasma (SP) before sorting affect sperm sortability and the ability of sex-sorted spermatozoa to tolerate liquid storage. Whole ejaculate samples were divided into three aliquots immediately after collection: one was diluted (1:1, v/v) in Beltsville thawing solution (BTS; 50% SP); the SP of the other two aliquots was removed and the sperm pellets were diluted with BTS + 10% of their own SP (10% SP) or BTS alone (0% SP). The three aliquots of each ejaculate were divided into two portions, one that was processed immediately for sorting and a second that was sorted after 24h storage at 15-17°C. In the first experiment, the ability to exhibit well-defined X- and Y-chromosome-bearing sperm peaks (split) in the cytometry histogram and the subsequent sorting efficiency were assessed (20 ejaculates). In contrast with holding time, the SP proportion influenced the parameters examined, as evidenced by the higher number of ejaculates exhibiting split and better sorting efficiency (P<0.05) in semen samples with 0-10% SP compared with those with 50% SP. In a second experiment, the quality (viability, total and progressive motility) and functionality (plasma membrane fluidity and intracellular generation of reactive oxygen species) of sex-sorted spermatozoa were evaluated after 0, 72 and 120h storage at 15-17°C (10 ejaculates). Holding time and SP proportion did not influence the quality or functionality of stored sex-sorted spermatozoa. In conclusion, a holding time as long as 24h before sorting did not negatively affect sex sorting efficiency or the ability of sorted boar spermatozoa to tolerate long-term liquid storage. A high proportion of SP (50%) in the semen samples before sorting reduced the number of ejaculates to be sorted and negatively influenced the sorting efficiency, but did not affect the ability of sex-sorted spermatozoa to tolerate liquid storage.
authors.affiliation: Department of Medicine and Animal Surgery, University of Murcia, Veterinary Medicine, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain.
authors.forename: Diego V
authors.initials: DV
authors.lastname: Alkmin
authors.affiliation: Department of Medicine and Animal Surgery, University of Murcia, Veterinary Medicine, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Parrilla
authors.affiliation: Department of Medicine and Animal Surgery, University of Murcia, Veterinary Medicine, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Tarantini
authors.affiliation: Department of Medicine and Animal Surgery, University of Murcia, Veterinary Medicine, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Del Olmo
authors.affiliation: Department of Medicine and Animal Surgery, University of Murcia, Veterinary Medicine, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Vazquez
authors.affiliation: Department of Medicine and Animal Surgery, University of Murcia, Veterinary Medicine, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain.
authors.forename: Emilio A
authors.initials: EA
authors.lastname: Martinez
authors.affiliation: Department of Medicine and Animal Surgery, University of Murcia, Veterinary Medicine, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Roca
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000662:veterinary; D002470:Cell Survival; D004542:Ejaculation; D005434:Flow Cytometry / Q000662:veterinary; D005819:Genetic Markers; D005838:Genotype; D008297:Male; D010641:Phenotype; D017382:Reactive Oxygen Species / Q000378:metabolism; D012661:Semen / Q000166:cytology*; D012662:Semen Preservation / Q000662:veterinary*; D012743:Sex Preselection / Q000662:veterinary*; D013048:Specimen Handling / Q000662:veterinary*; D013076:Sperm Count / Q000662:veterinary; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism / Q000502:physiology*; D034421:Sus scrofa; D013997:Time Factors; D014960:X Chromosome; D014998:Y Chromosome
chemical_list: D005819:Genetic Markers; D017382:Reactive Oxygen Species