title: Removal performance of nitrogen and endocrine-disrupting pesticides simultaneously in the enhanced biofilm system for polluted source water pretreatment.
abstract: The removal performances of nitrogen and trace levels of endocrine-disrupting pesticides (cypermethrin and chlorpyrifos) were studied in the enhanced biofilm pretreatment system at various substrates concentrations and dissolve oxygen (DO) niches. No significant change of EDPs removal occurred with the increased feed of ammonia nitrogen in aerobic batch tests or nitrate in anaerobic batch reactors, but significantly enhanced via reed addition both in aerobic and anaerobic conditions. Simultaneously enhanced denitrification and EDPs removal were achieved in the anoxic niche with reed addition. The results of denaturing gradient gel electrophoresis (DGGE) indicated that new bands appeared, and some bands became more intense with the reed addition. Sequences analysis showed that the dominant species belonged to Methylophilaceae, Hyphomicrobium, Bacillus and Thauera, which were related to the nitrogen or EDPs removals. In addition, the growth of functional heterotrophic microbes may be promoted via reed addition.
authors.affiliation: Department of Environmental Engineering, Zhejiang Ocean University, No. 1 Haida South Road, Zhoushan 316022, PR China; Department of Environmental Engineering, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Feng
authors.affiliation: Department of Environmental Engineering, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Guang-Feng
authors.initials: GF
authors.lastname: Yang
authors.affiliation: Department of Environmental Engineering, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Environmental Engineering, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, PR China; ZJU-UWA Joint Centre in Integrated Water Management and Protection, No. 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Xiang-Yang
authors.initials: XY
authors.lastname: Xu
mesh_terms: D001483:Base Sequence; D018441:Biofilms; D019149:Bioreactors; D004390:Chlorpyrifos / Q000737:chemistry; D058645:Denaturing Gradient Gel Electrophoresis; D058440:Denitrification; D052244:Endocrine Disruptors / Q000032:analysis / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009584:Nitrogen / Q000032:analysis / Q000302:isolation & purification*; D010575:Pesticides / Q000032:analysis / Q000302:isolation & purification*; D018547:Plant Stems / Q000378:metabolism; D011722:Pyrethrins / Q000737:chemistry; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000302:isolation & purification*; D018508:Water Purification / Q000379:methods*
chemical_list: D052244:Endocrine Disruptors; D010575:Pesticides; D011722:Pyrethrins; D014874:Water Pollutants, Chemical; C017160:cypermethrin; D004390:Chlorpyrifos; D009584:Nitrogen
keywords: Endocrine-disrupting pesticide; Microbial community; Nitrogen; Polluted source water; Reed addition