title: Intracellular signaling of CTLs.
abstract: Extracellular signals are transmitted to intracellular targets thanks to a complex network of interacting proteins that regulate a large number of cellular processes. The signaling mechanisms that occur in CTLs are initiated by T cell receptors (TCRs) that recognize antigens presented in the groove of class I histocompatibility (MHC) molecules of target cells. The strength of this interaction and the consequent activation of intracellular mechanisms determine the fate of the developing T cells. The intracellular mediators of activation can be easily detected using a classical methodology such as western blot. Here we describe this technique bearing particular attention to these mediators using SNAP i.d., a newly developed protein detection system.
authors.affiliation: Department of Emergency and Organ Transplantation, University of Bari, Piazza G. Cesare 11, 70124, Bari, Italy, sharonnatasha.cox@uniba.it.
authors.forename: Sharon Natasha
authors.initials: SN
authors.lastname: Cox
mesh_terms: D017209:Apoptosis / Q000276:immunology; D015153:Blotting, Western; D015550:Cell Degranulation / Q000276:immunology; D049109:Cell Proliferation; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D008213:Lymphocyte Activation / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology* / Q000502:physiology
chemical_list: D015395:Histocompatibility Antigens Class I; D011948:Receptors, Antigen, T-Cell