title: Detection of Mycobacterium tuberculosis in latently infected lungs by immunohistochemistry and confocal microscopy.
abstract: Detection of latent Mycobacterium tuberculosis is a challenge in the diagnosis of asymptomatic, subclinical tuberculosis. We report the development of an immunofluorescence technique to visualize and enumerate M. tuberculosis in latently infected rabbit lungs where no acid-fast-stained organisms were seen and no cultivable bacilli were obtained by the agar-plating method.
authors.affiliation: Public Health Research Institute (PHRI), Rutgers Biomedical and Health Sciences, Rutgers University, Newark, NJ, USA.|Laboratory of Mycobacterial Immunity and Pathogenesis, Rutgers University, Newark, NJ, USA.
authors.forename: Selvakumar
authors.initials: S
authors.lastname: Subbian
authors.affiliation: Public Health Research Institute (PHRI), Rutgers Biomedical and Health Sciences, Rutgers University, Newark, NJ, USA.
authors.forename: Eliseo
authors.initials: E
authors.lastname: Eugenin
authors.affiliation: The Bill & Melinda Gates Foundation, Seattle, WA, USA.|Public Health Research Institute (PHRI), Rutgers Biomedical and Health Sciences, Rutgers University, Newark, NJ, USA.|Laboratory of Mycobacterial Immunity and Pathogenesis, Rutgers University, Newark, NJ, USA.
authors.forename: Gilla
authors.initials: G
authors.lastname: Kaplan
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D007150:Immunohistochemistry / Q000379:methods*; D008168:Lung / Q000276:immunology; D018613:Microscopy, Confocal / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D011817:Rabbits; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*