title: Anti-inflammatory effect of chlorogenic acid on the IL-8 production in Caco-2 cells and the dextran sulphate sodium-induced colitis symptoms in C57BL/6 mice.
abstract: Chlorogenic acid (CHA) is an antioxidant polyphenol prevalent in human diet, with coffee, fruits, and vegetables being its main source. Effects of CHA and CHA metabolites were evaluated on the IL-8 production in human intestinal Caco-2 cells induced by combined stimulation with tumour necrosis factor alpha (TNFα) and H2O2. CHA and caffeic acid (CA) inhibited TNFα- and H2O2-induced IL-8 production. We also examined the in vivo effects of CHA and CA using dextran sulphate sodium (DSS)-induced colitis in mice. CHA attenuated DSS-induced body weight loss, diarrhea, fecal blood, and shortening of colon and dramatically improved colitis histological scores. Furthermore, increases in the mRNA expression of colonic macrophage inflammatory protein 2 and IL-1β, which were induced by DSS, were significantly suppressed by CHA supplementation. These results suggest that dietary CHA use may aid in the prevention of intestinal inflammatory conditions.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan; Functional Materials Research Group, Division of Metabolism and Functionality Research, Korea Food Research Institute, Republic of Korea.
authors.forename: Hee Soon
authors.initials: HS
authors.lastname: Shin
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan; Department of Biotechnology, Faculty of Engineering, Maebashi Institute of Technology, Gunma, Japan. Electronic address: satsu@maebashi-it.ac.jp.
authors.forename: Hideo
authors.initials: H
authors.lastname: Satsu
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan; Institute for Basic Science, School of Biological Sciences, Seoul National University, Seoul, South Korea.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Bae
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Haru
authors.initials: H
authors.lastname: Ogiwara
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Totsuka
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan; Department of Nutritional Science, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000494:pharmacology; D018938:Caco-2 Cells; D002726:Chlorogenic Acid / Q000008:administration & dosage* / Q000494:pharmacology; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D016264:Dextran Sulfate / Q000009:adverse effects; D005260:Female; D006801:Humans; D053583:Interleukin-1beta / Q000276:immunology; D016209:Interleukin-8 / Q000276:immunology*; D007422:Intestines / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D002726:Chlorogenic Acid; D016264:Dextran Sulfate
keywords: Caffeic acid; Chlorogenic acid; Colitis; Dextran sulphate sodium; IL-8