title: Heterologous expression of AtPAP2 in transgenic potato influences carbon metabolism and tuber development.
abstract: Changes in carbon flow and sink/source activities can affect floral, architectural, and reproductive traits of plants. In potato, overexpression (OE) of the purple acid phosphatase 2 of Arabidopsis (AtPAP2) resulted in earlier flowering, faster growth rate, increased tubers and tuber starch content, and higher photosynthesis rate. There was a significant change in sucrose, glucose and fructose levels in leaves, phloem and sink biomass of the OE lines, consistent with an increased expression of sucrose transporter 1 (StSUT1). Furthermore, the expression levels and enzyme activity of sucrose-phosphate synthase (SPS) were also significantly increased in the OE lines. These findings strongly suggest that higher carbon supply from the source and improved sink strength can improve potato tuber yield.
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany; School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors.forename: Youjun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Sun
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, Golm, Germany.
authors.forename: Joerg
authors.initials: J
authors.lastname: Fettke
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany.
authors.forename: Mark Aurel
authors.initials: MA
authors.lastname: Schöttler
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors.forename: Lawrence
authors.initials: L
authors.lastname: Ramsden
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany. Electronic address: Fernie@mpimp-golm.mpg.de.
authors.forename: Alisdair R
authors.initials: AR
authors.lastname: Fernie
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China. Electronic address: bllim@hku.hk.
authors.forename: Boon Leong
authors.initials: BL
authors.lastname: Lim
mesh_terms: D000135:Acid Phosphatase / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D005632:Fructose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D035281:Plant Tubers / Q000254:growth & development* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D011198:Solanum tuberosum / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D006023:Glycoproteins; D026901:Membrane Transport Proteins; D010940:Plant Proteins; C078729:sucrose transport protein, plant; D005632:Fructose; D013395:Sucrose; D005964:Glucosyltransferases; C022698:sucrose-phosphate synthase; C091137:purple acid phosphatase; D000135:Acid Phosphatase; D005947:Glucose
keywords: AtPAP2; Photosynthesis; Potato; Sugar efflux; Tuber yield