title: Assessment of ovarian cancer conditions from exhaled breath.
abstract: We present a pilot study that aims to examine the possibility to easily and noninvasively detect and discriminate females with ovarian cancer (OC) from females that have no tumor(s) and from females that have benign genital tract neoplasia, using exhaled breath samples. The study is based on clinical samples and data from 182 females, as follows: 48 females with OC, 48 tumor-free controls and 86 females with benign gynecological neoplasia. Analysis of the breath samples with gas chromatography linked with mass spectrometry shows that decanal, nonanal, styrene, 2-butanone and hexadecane could serve as potential volatile markers for OC. Analysis of the same samples with tailor-made nanoarrays shows good discrimination between females with OC and females that have either no tumor or benign genital tract neoplasia (71% for accuracy, sensitivity and specificity). Conversely, the nanoarray output shows excellent discrimination between the OC patients and the tumor-free controls (79% sensitivity, 100% specificity and 89% accuracy). These results suggest that the nanoarray approach might be useful to avoid unnecessary complicated or expensive tests for tumor-free females in case of a negative result. In the case of positive result, the test will indicate with high probability the presence of OC.
authors.affiliation: Department of Chemical Engineering and Russell Berrie Nanotechnology Institute, Technion-Israel Institute of Technology, Haifa, 3200003, Israel.
authors.forename: Haitham
authors.initials: H
authors.lastname: Amal
authors.forename: Da-You
authors.initials: DY
authors.lastname: Shi
authors.forename: Radu
authors.initials: R
authors.lastname: Ionescu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Qing-Ling
authors.initials: QL
authors.lastname: Hua
authors.forename: Yue-Yin
authors.initials: YY
authors.lastname: Pan
authors.forename: Li
authors.initials: L
authors.lastname: Tao
authors.forename: Hu
authors.initials: H
authors.lastname: Liu
authors.forename: Hossam
authors.initials: H
authors.lastname: Haick
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000426:Alcohol Dehydrogenase / Q000032:analysis; D001944:Breath Tests; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000378:metabolism*; D012372:ROC Curve; D055549:Volatile Organic Compounds / Q000032:analysis
chemical_list: D055549:Volatile Organic Compounds; D000426:Alcohol Dehydrogenase
keywords: breath; diagnosis; ovarian cancer; sensor; volatile organic compound