title: Cell block technique as an additional tool in the diagnosis of ameloblastoma.
abstract: The objective of this study was to evaluate the cytological content of ameloblastomas of the jaw. Nine cases of ameloblastoma were punctured, and the intralesional material was processed using the cell block technique. After centrifugation, the pellet obtained from the punctured material was fixed in formaldehyde and routinely processed to inclusion in paraffin. The obtained sections were stained with haematoxylin and eosin (H&E). Immunohistochemical reactions against anti-pan-cytokeratin (AE1/AE3) were performed to measure the presence of epithelial cells. Cytological analyses of the obtained slides revealed the presence of epithelial cells (as evidenced by AE1/AE3 labelling) and acellular amorphous eosinophilic materials. These cytological findings, in light of clinical and imaging data, can be helpful in the presumptive diagnosis of this disease entity by eliminating other possible diagnoses. Nonetheless further studies are needed in order to determine the nature of the acellular amorphous eosinophilic material and to ascertain the immunoprofile of epithelial cells.
authors.affiliation: Postgraduate Program in Dentistry, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
authors.forename: Maria Fernanda da Silva
authors.initials: MF
authors.lastname: Belatto
authors.affiliation: Postgraduate Program in Dentistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Grasieli de Oliveira
authors.initials: Gde O
authors.lastname: Ramos
authors.affiliation: Department of Oral Pathology, School of Dentistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Manoela Domingues
authors.initials: MD
authors.lastname: Martins
authors.affiliation: Department of Pathology, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
authors.forename: Liliane Janete
authors.initials: LJ
authors.lastname: Grando
authors.affiliation: Department of Pathology, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
authors.forename: Elena Riet Correa
authors.initials: ER
authors.lastname: Rivero
mesh_terms: D000564:Ameloblastoma / Q000473:pathology*; D044963:Biopsy, Fine-Needle; D003581:Cytodiagnosis / Q000379:methods; D004847:Epithelial Cells / Q000473:pathology; D004912:Erythrocytes / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007573:Jaw Neoplasms / Q000473:pathology*; D007633:Keratins / Q000276:immunology; D009808:Odontogenic Tumors / Q000473:pathology*; D016612:Paraffin Embedding
chemical_list: D007633:Keratins