title: Investigating TLR signaling responses in murine dendritic cells upon bacterial infection.
abstract: Innate immune recognition of microbial components is critical for the onset of an appropriate immune response against invading pathogens, in particular by dendritic cells. Toll-like receptors (TLRs) are key in the detection of a variety of microbial stimuli.Here we focus on the methodology used to evaluate the role of TLRs in the process of dendritic cell response to bacterial intracellular infections, using bone marrow-derived dendritic cells (BMDCs) as a model system. This protocol describes how to access the level of activation of BMDCs using standard immunology and biochemistry approaches along with examination of infected cells by immunofluorescence microscopy.
authors.affiliation: Bases Moléculaires et Structurales des Systèmes Infectieux, CNRS UMR 5086, Institut de Biologie et Chimie des Protéines, Université Lyon 1, IBCP, 69367, Lyon, France, suzana.salcedo@ibcp.fr.
authors.forename: Suzana Pinto
authors.initials: SP
authors.lastname: Salcedo
authors.forename: Lena
authors.initials: L
authors.lastname: Alexopoulou
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology; D003713:Dendritic Cells / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence; D015398:Signal Transduction / Q000502:physiology; D051193:Toll-Like Receptors / Q000378:metabolism*
chemical_list: D051193:Toll-Like Receptors