title: Antioxidant activity and protective effect of extract of Celosia cristata L. flower on tert-butyl hydroperoxide-induced oxidative hepatotoxicity.
abstract: This study was undertaken to evaluate the antioxidant potential and protective effects of Celosia cristata L. (Family: Amaranthaceae) flower (CCF) extracts on tert-butyl-hydroperoxide (t-BHP)-induced oxidative damage in the hepatocytes of Chang cells and rat livers. In vitro, CCF extracts exhibited protective effect through their radical scavenging ability to enhance cell viability, prevent reactive oxygen species (ROS) generation, and inhibit mitochondrial membrane depolarisation in t-BHP-induced hepatotoxicity in Chang cells. In vivo, oral feeding of CCF (100mg and 500mg/kg of body weight) to rats for five consecutive days before a single dose of t-BHP (2mmol/kg, i.p.) showed a significant (p<0.05) protective effect by lowering serum levels of glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT). The extract decreased the hepatic levels of lipid peroxidation (MDA) and serum level of triglyceride (TG) against t-BHP-induced oxidative stress. These results indicate that CCF extract prevented oxidative stress-induced liver injury by enhancing hepatocyte antioxidant abilities.
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea; Department of Applied Life Science, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea.
authors.forename: Yon-Suk
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea; Department of Applied Life Science, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea.
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Hwang
authors.affiliation: Nokyong Research Center, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea.
authors.forename: Si-Heung
authors.initials: SH
authors.lastname: Sung
authors.affiliation: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
authors.affiliation: Department of Food & Biotechnology, Korea National University of Transportation, Chungju 380-702, Republic of Korea.
authors.forename: Jae-Hyun
authors.initials: JH
authors.lastname: Jeong
authors.affiliation: Nokyong Research Center, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea.
authors.forename: Byong-Tae
authors.initials: BT
authors.lastname: Jeon
authors.affiliation: Nokyong Research Center, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea.
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Moon
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea; Department of Applied Life Science, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea; Nokyong Research Center, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea. Electronic address: parkpj@kku.ac.kr.
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D034762:Celosia / Q000737:chemistry* / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology* / Q000517:prevention & control; D035264:Flowers / Q000737:chemistry / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000633:toxicity
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D020011:Protective Agents; D017382:Reactive Oxygen Species; D020122:tert-Butylhydroperoxide
keywords: Antioxidant activity; Celosia cristata L; Chang cells; Free radical; Oxidative stress; t-BHP