title: Localization of GATA2 in the nuclear and cytoplasmic regions of ovine conceptuses.
abstract: GATA transcription factors are emerging as critical regulators in trophoblast development and its gene regulation. The purpose of this study was to examine the expression and cellular localization of GATA2 in ovine conceptuses during the peri-implantation period. In Western blot analyses, GATA2 proteins were found in days 15, 17 and 21 ovine conceptuses (day 0=day of estrus). Using immunohistochemistry and immunofluorescence analyses, we found that GATA2 was localized in days 15, 17 and 21 ovine conceptuses, and more importantly, GATA2 protein was detected in both nuclear and cytoplasmic regions of the trophectoderm. To our knowledge, the present study is the first to demonstrate that GATA2 is localized in two cellular compartments of the trophectoderm in ovine and many other mammalian species, and suggests that the difference in GATA2 location plays a role in the regulation of down-stream genes during the early pregnancy period.
authors.affiliation: Laboratory of Theriogenology and Animal Breeding, Graduate School of Agricultural and Life Science, The University of Tokyo, Tokyo, Japan.
authors.forename: Hanako
authors.initials: H
authors.lastname: Bai
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Sakurai
authors.forename: Rulan
authors.initials: R
authors.lastname: Bai
authors.forename: James D
authors.initials: JD
authors.lastname: Godkin
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Imakawa
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D010064:Embryo Implantation; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D050989:GATA2 Transcription Factor; D050991:GATA4 Transcription Factor / Q000032:analysis / Q000378:metabolism* / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D011247:Pregnancy; D012756:Sheep / Q000196:embryology* / Q000235:genetics*; D014327:Trophoblasts / Q000166:cytology*
chemical_list: D050989:GATA2 Transcription Factor; D050991:GATA4 Transcription Factor
keywords: GATA2; localization; ovine conceptuses