title: Gene expression profiling of sesaminol triglucoside and its tetrahydrofuranoid metabolites in primary rat hepatocytes.
abstract: Sesaminol triglucoside is a major lignin in sesame meal and has a methylenedioxyphenyl group and multiple functions in vivo. As a tetrahydrofurofuran type lignan, sesaminol triglucoside is metabolized to mammalian lignans. This investigation studies the effect of sesaminol triglucoside and its tetrahydrofuranoid metabolites (sesaminol, 2-episesaminol, hydroxymethyl sesaminol-tetrahydrofuran, enterolactone, and enterodiol) on gene expression in primary rat hepatocytes using a DNA microarray. Sesame lignans significantly affected the expression of xenobiotic-induced transcripts of cytochrome P450, solute carrier (SLC), and ATP-binding cassette (ABC) transporters. Changes in gene expression were generally greater in response to metabolites with methylenedioxyphenyl moieties (sesaminol triglucoside, sesaminol, and 2-episesaminol) than to the tetrahydrofuranoid metabolites (hydroxymethyl sesaminol-tetrahydrofuran, enterolactone, and enterodiol). Tetrahydrofuran lignans, such as sesaminol triglucoside, sesamin, hydroxymethyl sesaminol-tetrahydrofuran, and sesaminol changed the expression of ABC transporters.
authors.affiliation: Food Industry Research & Development Institute , Hsinchu , Taiwan and.
authors.forename: Kuo-Ching
authors.initials: KC
authors.lastname: Jan
authors.forename: Binghuei-Barry
authors.initials: BB
authors.lastname: Yang
authors.forename: Tristan C
authors.initials: TC
authors.lastname: Liu
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000378:metabolism; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004149:Dioxoles / Q000378:metabolism* / Q000494:pharmacology; D005663:Furans / Q000378:metabolism* / Q000494:pharmacology; D015870:Gene Expression; D020869:Gene Expression Profiling; D005960:Glucosides / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism; D008658:Inactivation, Metabolic / Q000187:drug effects / Q000235:genetics; D017705:Lignans / Q000378:metabolism* / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D008322:Mammals; D010936:Plant Extracts / Q000378:metabolism / Q000494:pharmacology; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry; D031685:Sesamum / Q000737:chemistry*
chemical_list: D018528:ATP-Binding Cassette Transporters; D004149:Dioxoles; D005663:Furans; D005960:Glucosides; D017705:Lignans; D010936:Plant Extracts; C518861:sesaminol triglucoside; C018674:tetrahydrofuran; C066016:sesaminol; C029498:2,3-bis(3'-hydroxybenzyl)butane-1,4-diol; D003577:Cytochrome P-450 Enzyme System; D015107:4-Butyrolactone; C054125:sesamin; C029497:2,3-bis(3'-hydroxybenzyl)butyrolactone
keywords: Cytochrome P450; gene expression; microarray; sesaminol triglucoside; tetrahydrofuranoid lignans