title: Hox transcriptomics in Drosophila embryos.
abstract: Hox proteins are evolutionarily conserved homeodomain containing transcription factors that specify segment identities along the anteroposterior axis of almost all bilaterian animals. They exert their morphogenetic role by transcriptionally regulating a large battery of downstream target genes. Therefore the dissection of transcriptional networks regulated by Hox proteins is an essential step towards a mechanistic understanding of how these transcription factors coordinate multiple developmental and morphogenetic processes. High-throughput techniques allowing whole-transcriptome mRNA expression profiling are powerful tools for the genome-wide identification of Hox downstream target genes in a variety of experimental settings. Here, we describe how to quantitatively identify Hox downstream genes in Drosophila embryos by performing a Hox transcriptome analysis using microarrays.
authors.affiliation: Centre for Organismal Studies (COS) Heidelberg, University of Heidelberg, Im Neuenheimer Feld 267, 69120, Heidelberg, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Polychronidou
authors.forename: Ingrid
authors.initials: I
authors.lastname: Lohmann
mesh_terms: D000818:Animals; D004330:Drosophila / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods; D018398:Homeodomain Proteins / Q000235:genetics*; D059467:Transcriptome
chemical_list: D018398:Homeodomain Proteins