title: Efficient arachidonic acid-rich oil production by Mortierella alpina through a repeated fed-batch fermentation strategy.
abstract: Arachidonic acid (ARA)-rich oil production by Mortierella alpina is a long fermentation period needed process due to the low growth rate of the filamentous fungus used. This causes the low productivity of ARA-rich oil and hinders its industrial mass scale production. In the present study, different fed-batch strategies were conducted to shorten the fermentation period. The result showed that compared with the batch culture, the fermentation period was shortened from 7days to 5days with the productivity of ARA-rich oil increased from 0.9g/(L·d) to 1.3g/(L·d) by using the fed-batch fermentation strategy. Furthermore, repeated fed-batch fermentation strategy was adopted to achieve the purpose of continuous production. By using this strategy, the fermentation period was shortened from 40days to 26days in a four cycle repeated fed-batch fermentation. This strategy proved to be convenient and economical for ARA-rich oil commercial production process.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China. Electronic address: xiaojunji@njtech.edu.cn.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
authors.forename: Ai-Hui
authors.initials: AH
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
authors.forename: Zhi-Kui
authors.initials: ZK
authors.lastname: Nie
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
authors.forename: Wen-Jia
authors.initials: WJ
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
authors.forename: Lu-Jing
authors.initials: LJ
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China. Electronic address: biotech@njtech.edu.cn.
authors.forename: He
authors.initials: H
authors.lastname: Huang
mesh_terms: D016718:Arachidonic Acid / Q000096:biosynthesis*; D019149:Bioreactors; D018929:Cell Culture Techniques; D005285:Fermentation / Q000502:physiology*; D008401:Gas Chromatography-Mass Spectrometry; D007218:Industrial Microbiology / Q000379:methods*; D020094:Mortierella / Q000378:metabolism / Q000502:physiology*; D009821:Oils / Q000032:analysis*; D013997:Time Factors
chemical_list: D009821:Oils; D016718:Arachidonic Acid
keywords: Arachidonic acid; Lipid; Mortierella alpina; Productivity; Repeated fed-batch