title: Adipose stem cells-conditioned medium blocks 6-hydroxydopamine-induced neurotoxicity via the IGF-1/PI3K/AKT pathway.
abstract: Previous studies suggest that the delivery of neurotrophic factors secreted from adipose stromal cells (ASC) protect the brain from 6-hydroxydopamine (6-OHDA)-induced neurotoxicity. However, it remains unclear which secreted neurotrophic factor has an important role in protecting 6-OHDA-treated neurons. Through the use of antibodies in this study, we demonstrated that specific neutralization of IGF-1 activity in ASC conditioned media (ASC-CM) significantly blocks ASC-CM-induced neuroprotection against 6-OHDA neurotoxicity. Consistently, this neuroprotection was mostly attributed to the activation of the AKT-mediated signaling pathway. In contrast, brain derived neurotrophic factor (BDNF), glial-derived neurotrophic factor (GDNF), vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) in ASC-CM did not play a role in ASC-CM-induced neuroprotection against 6-OHDA.
authors.affiliation: Department of Neurology, Linyi People's Hospital, No. 49 Yizhou Road, Linyi, Shandong 276000, P.R. China.
authors.forename: Xianjun
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Neurology, Linyi People's Hospital, No. 49 Yizhou Road, Linyi, Shandong 276000, P.R. China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Neurology, Linyi People's Hospital, No. 49 Yizhou Road, Linyi, Shandong 276000, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gong
authors.affiliation: Department of Neurology, Qilu Hospital of Shandong University and Bain Science Research Institute, Shandong University, No. 107 Wenhuaxi Road, Jinan, Shandong 250012, P.R. China.
authors.forename: Shengnian
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Pediatrics, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, P.R. China. Electronic address: hjpeng1975@hotmail.com.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Peng
authors.affiliation: Department of Neurology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
authors.forename: Adam
authors.initials: A
authors.lastname: Shatara
authors.affiliation: Department of Neurology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
authors.forename: Timmy Z
authors.initials: TZ
authors.lastname: Zhu
authors.affiliation: Department of Neurology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Meltzer
authors.affiliation: Department of Neurology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Neurology, Indiana University School of Medicine, Indianapolis, IN 46202, USA. Electronic address: huiygu@iupui.edu.
authors.forename: Huiying
authors.initials: H
authors.lastname: Gu
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D000906:Antibodies; D017209:Apoptosis; D002470:Cell Survival; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000276:immunology / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D016627:Oxidopamine / Q000633:toxicity*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D000906:Antibodies; D036341:Intercellular Signaling Peptides and Proteins; D007334:Insulin-Like Growth Factor I; D016627:Oxidopamine; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt
keywords: 6-hydroxydopamine; AKT; ASC-CM; IGF-1; Neuronal cell death