title: Cleavage of hemagglutinin-bearing lentiviral pseudotypes and their use in the study of influenza virus persistence.
abstract: Influenza A viruses (IAVs) are a major cause of infectious respiratory human diseases and their transmission is dependent upon the environment. However, the role of environmental factors on IAV survival outside the host still raises many questions. In this study, we used lentiviral pseudotypes to study the influence of the hemagglutinin protein in IAV survival. High-titered and cleaved influenza-based lentiviral pseudoparticles, through the use of a combination of two proteases (HAT and TMPRSS2) were produced. Pseudoparticles bearing hemagglutinin proteins derived from different H1N1, H3N2 and H5N1 IAV strains were subjected to various environmental parameters over time and tested for viability through single-cycle infectivity assays. We showed that pseudotypes with different HAs have different persistence profiles in water as previously shown with IAVs. Our results also showed that pseudotypes derived from H1N1 pandemic virus survived longer than those derived from seasonal H1N1 virus from 1999, at high temperature and salinity, as previously shown with their viral counterparts. Similarly, increasing temperature and salinity had a negative effect on the survival of the H3N2 and H5N1 pseudotypes. These results showed that pseudotypes with the same lentiviral core, but which differ in their surface glycoproteins, survived differently outside the host, suggesting a role for the HA in virus stability.
authors.affiliation: Institut Pasteur, Environment and Infectious Risks Research and Expertise Unit, Laboratory for Urgent Response to Biological Threats, Paris, France; University of Paris Diderot, Sorbonne Paris Cité (Cellule Pasteur), Paris, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Sawoo
authors.affiliation: Institut Pasteur, Environment and Infectious Risks Research and Expertise Unit, Laboratory for Urgent Response to Biological Threats, Paris, France.
authors.forename: Amélie
authors.initials: A
authors.lastname: Dublineau
authors.affiliation: Institut Pasteur, Environment and Infectious Risks Research and Expertise Unit, Laboratory for Urgent Response to Biological Threats, Paris, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Batéjat
authors.affiliation: Institut Pasteur of Shanghai, Unit of Antiviral Immunity and Genetic Therapy, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Paul
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Institut Pasteur, Environment and Infectious Risks Research and Expertise Unit, Laboratory for Urgent Response to Biological Threats, Paris, France.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Manuguerra
authors.affiliation: Institut Pasteur, Environment and Infectious Risks Research and Expertise Unit, Laboratory for Urgent Response to Biological Threats, Paris, France; University of Paris Diderot, Sorbonne Paris Cité (Cellule Pasteur), Paris, France.
authors.forename: India
authors.initials: I
authors.lastname: Leclercq
mesh_terms: D000818:Animals; D004285:Dogs; D057809:HEK293 Cells; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D009980:Influenza A virus / Q000145:classification* / Q000235:genetics / Q000502:physiology*; D016086:Lentivirus / Q000235:genetics / Q000502:physiology*; D061985:Madin Darby Canine Kidney Cells; D054712:Salinity; D013312:Stress, Physiological; D014867:Water
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D014867:Water