title: Ortleppascaris sp. and your host Rhinella marina: A proteomic view into a nematode-amphibian relationship.
abstract: The success of the helminth-host relationship depends on a biochemical molecular arsenal. Perhaps the proteome is the largest and most important set of this weaponry, in which the proteins have a crucial role in vital processes to the parasite/host relationship, from basic metabolism and energy production to complex immune responses. Nowadays, the bioproducts expressed by the parasites are under the "spotlight" of immunoassays and biochemical analysis in helminthology, especially in proteomic analysis, which has provided valuable information about the physiology of the infecting agent. Looking into this point of view, why not turn to the infected agent as well? This study characterised the proteomic profile of fluid-filled fibrous cysts of encapsulated Ortleppascaris sp. larvae in the hepatic parenchyma of their intermediate host, the amphibian Rhinella marina. The proteins were separated by two-dimensional electrophoresis and identified by MS with the aid of Peptide Mass Fingerprint. A total of 54 molecules were analysed in this system, revealing a complex protein profile with molecules related to basic metabolic processes of the parasite, energy production, oxi-reduction and oxidative stress processes as well as molecules related to the host response. This study contributes to proteomic studies of protein markers of the development, infectivity, virulence and co-existence of helminths and their hosts.
authors.affiliation: Laboratory of Cell Biology and Helminthology (Laboratório de Biologia Celular e Helmintologia) "Profa. Dra. Reinalda Marisa Lanfredi", Biological Sciences Institute (Instituto de Ciências Biológicas), Federal University of Pará (Universidade Federal do Pará), Belém, Pará, Brazil.
authors.forename: Jefferson Pereira E
authors.initials: JP
authors.lastname: Silva
authors.affiliation: Laboratory of Cell Biology and Helminthology (Laboratório de Biologia Celular e Helmintologia) "Profa. Dra. Reinalda Marisa Lanfredi", Biological Sciences Institute (Instituto de Ciências Biológicas), Federal University of Pará (Universidade Federal do Pará), Belém, Pará, Brazil.
authors.forename: Adriano Penha
authors.initials: AP
authors.lastname: Furtado
authors.affiliation: Laboratory of Cell Biology and Helminthology (Laboratório de Biologia Celular e Helmintologia) "Profa. Dra. Reinalda Marisa Lanfredi", Biological Sciences Institute (Instituto de Ciências Biológicas), Federal University of Pará (Universidade Federal do Pará), Belém, Pará, Brazil.
authors.forename: Jeannie Nascimento Dos
authors.initials: JN
authors.lastname: Santos
keywords: Helminth; Host–parasite; Ortleppascaris; Proteomic; Relationship; Rhinella marina