title: HIV-1 drug resistance mutations among infants born to HIV-positive mothers in Busia, Kenya.
abstract: To determine HIV-1 subtypes and transmitted HIV-1 drug-resistant mutations among HIV-1-positive children born to HIV-positive mothers in Busia County, blood samples were collected from 53 children aged between 6 weeks and 5 years in 2011. Their mothers were HIV-1 positive and on antiretroviral therapy at the time the children were born. The samples were analyzed for HIV-1 drug resistance and subtypes through sequencing of portions of the HIV-1 pol gene. The generated sequences were analyzed for subtype diversity using the REGA and BLAST subtyping tools. HIV-1 drug resistance was determined using the Stanford University HIV database. Of the 53 samples that were successfully amplified and sequenced, 69.8% (37/53) were determined to be HIV-1 subtype A, 22.6% (12/53) were subtype D, 5.6% (3/53) were subtype C, and 1.8% (1/53) were subtype A1C. The prevalence of HIV-1 drug resistance mutations of any kind was 22.6% (12/53).
authors.affiliation: 1 Centre for Virus Research, Kenya Medical Research Institute , Nairobi, Kenya .
authors.forename: Rency
authors.initials: R
authors.lastname: Lel
authors.forename: Jane
authors.initials: J
authors.lastname: Ngaira
authors.forename: Raphael
authors.initials: R
authors.lastname: Lihana
authors.forename: Samoel
authors.initials: S
authors.lastname: Khamadi
mesh_terms: D019380:Anti-HIV Agents / Q000627:therapeutic use; D002675:Child, Preschool; D024882:Drug Resistance, Viral / Q000235:genetics; D005260:Female; D015658:HIV Infections / Q000188:drug therapy / Q000635:transmission / Q000821:virology*; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D018445:Infectious Disease Transmission, Vertical; D007630:Kenya / Q000453:epidemiology; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology; D015995:Prevalence
chemical_list: D019380:Anti-HIV Agents