title: [Establishment and functional identification of a stable cell line overexpression heme oxygenase-1].
abstract: OBJECTIVE
  To establish a stable cell line overexpression heme oxygenase-1 (HO-1) mediated by a modified lentivirus system and identify its function.
  
  
  METHODS
  The HO-1 gene was amplified by polymerase chain reaction and cloned into the modified pLentiLox3.7 expression vectors. The recombinant plasmids were transfected into HEK293T cells and the HO-1 was detected by Western blot. The recombinant plasmids were transfected into HEK293T cells to produce the viruses, with the helping plasmids including plp1, plp2, and VSVG. HEK293T cells were infected by the viruses and the cells that can express HO-1 were identified by Western blot. The reactive oxygen species were detected in the HO-1-overexpression HEK293T cells and the normal cells after the adding of hydrogen peroxide. The same experiment was performed with human umbilical vein endothelial cells.
  
  
  RESULTS
  The stable cell line that can overexpress HO-1 was established, which was verified by Western blot. The reactive oxygen species in the HO-1-overexpression HEK293T cells and human umbilical vein endothelial cells decreased obviously after exposure to hydrogen peroxide.
  
  
  CONCLUSIONS
  The lentivirus-carrying HO-1 was successfully packaged and the stable cell line overexpression HO-1 was established. HO-1 can play a protective role in the course of oxidative damage.
authors.affiliation: Department of Vascular Surgery,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
authors.forename: Gen-huan
authors.initials: GH
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Medical Molecular Biology,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing 100005,China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Medical Molecular Biology,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing 100005,China.
authors.forename: Yan-chuan
authors.initials: YC
authors.lastname: Li
authors.affiliation: Department of Vascular Surgery,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
authors.forename: Leng
authors.initials: L
authors.lastname: Ni
authors.affiliation: Department of Vascular Surgery,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
authors.forename: Zhan-qi
authors.initials: ZQ
authors.lastname: Wang
authors.affiliation: Department of Vascular Surgery,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
authors.forename: Xi-tao
authors.initials: XT
authors.lastname: Song
authors.affiliation: Department of Vascular Surgery,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
authors.forename: Chang-wei
authors.initials: CW
authors.lastname: Liu
mesh_terms: D002460:Cell Line; D057809:HEK293 Cells; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D010957:Plasmids; D014162:Transfection
chemical_list: C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1