title: HN1 negatively influences the β-catenin/E-cadherin interaction, and contributes to migration in prostate cells.
abstract: Previously, it has been reported that HN1 is involved in cytoplasmic retention and degradation of androgen receptor in an AKT dependent manner. As HN1 is a hormone inducible gene, and has been shown that it is upregulated in various cancers, we studied the importance of HN1 function in β-catenin signaling in prostate cancer cell line, PC-3 and mammary cancer cell line MDA-MB231. Here, we demonstrated that HN1 physically associates with GSK3β/β-catenin destruction complex and abundantly localizes to cytoplasm, especially when the GSK3β is phosphorylated on S9 residue. Further, ectopic HN1 expression results an increase in the β-catenin degradation leading to loss of E-cadherin interaction, concurrently contributing to actin re-organization, colony formation and migration in cancer cell lines. Thus, we report that HN1 is an essential factor for β-catenin turnover and signaling, augments cell growth and migration in prostate cancer cells.
authors.affiliation: Department of Bioengineering, Faculty of Engineering, Cancer Biology Laboratory, Ege University, Bornova, Izmir, Turkey; Department of Biology, Art and Science Faculty, Harran University, Osmanbey Campus, Sanliurfa, Turkey.
authors.forename: Lokman
authors.initials: L
authors.lastname: Varisli
authors.forename: Bilge E
authors.initials: BE
authors.lastname: Ozturk
authors.forename: Gencer K
authors.initials: GK
authors.lastname: Akyuz
authors.forename: Kemal S
authors.initials: KS
authors.lastname: Korkmaz
mesh_terms: D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins; D011467:Prostate / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: D015820:Cadherins; D018797:Cell Cycle Proteins; C572206:JPT1 protein, human; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D051176:beta Catenin
keywords: ADHERENT JUNCTION; GSK3β; HN1; β-CATENIN