title: Fabrication of surface plasmon resonance sensor surface with control of the non-specific adsorption and affinity for the detection of 2,4,6-trinitrotoluene using an antifouling copolymer.
abstract: We fabricated a surface plasmon resonance (SPR) sensor using a hydrophilic polymer for the highly sensitive detection of 2,4,6-trinitrotoluene (TNT). The hydrophilic polymer was made from mono-2-(methacryloyloxy)ethylsuccinate (MES) and 2-hydroxyethylmethacrylate (HEMA) by surface-initiated atom transfer radical polymerization. The detection of TNT was carried out by displacement assay with the SPR measurement. In displacement assay, the affinity between anti-TNT antibody and the sensor surface, affects to the sensitivity. In the SPR measurement, non-specific adsorption should be controlled because SPR sensor cannot discriminate between specific and non-specific adsorption. Therefore, the affinity and non-specific adsorption were controlled by changing the ratio of HEMA to MES. A detection limit of 0.4 ng/ml (ppb) for TNT was achieved using a sensor surface with the lowest affinity without non-specific adsorption.
authors.affiliation: Research and Development Center for Taste and Odor Sensing, Kyushu University , Fukuoka , Japan.
authors.forename: Rui
authors.initials: R
authors.lastname: Yatabe
authors.affiliation: Research and Development Center for Taste and Odor Sensing, Kyushu University , Fukuoka , Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Onodera
authors.affiliation: Graduate School of Information Science and Electrical Engineering, Kyushu University , Fukuoka , Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Toko
keywords: displacement assay; immunosensor; non-specific adsorption; self-assembled monolayer; surface plasmon resonance; surface-initiated atom transfer radical polymerization; trinitrotoluene