title: ¹³C-metabolic flux analysis for Escherichia coli.
abstract: (13)C-Metabolic flux analysis ((13)C-MFA) is used here to study the effects of the knockout of such genes as pgi, zwf, gnd, ppc, pck, pyk, and lpdA on the metabolic changes in Escherichia coli cultivated under aerobic condition. The metabolic regulation mechanisms were clarified by integrating such information as fermentation data, gene expression, enzyme activities, and metabolite concentrations as well the result of (13)C-MFA.
authors.affiliation: Kyushu Institute of Technology, 680-4 Kawazu, Iizuka-shi, Fukuoka, 820-8502, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Matsuoka
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Shimizu
mesh_terms: D000332:Aerobiosis; D002247:Carbon Isotopes / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics; D005285:Fermentation; D055786:Gene Knockout Techniques; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics; D064688:Metabolic Flux Analysis / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000235:genetics* / Q000502:physiology; D008954:Models, Biological
chemical_list: D002247:Carbon Isotopes; D029968:Escherichia coli Proteins; D005956:Glucose-6-Phosphate Isomerase; C554958:pgi protein, E coli