title: Survey of autoantibody responses against tumor-associated antigens in thyroid cancer.
abstract: BACKGROUND
  Autoantibodies against tumor-associated antigens (TAAs) have been shown to serve as highly specific serological biomarkers for the diagnosis of various solid cancers. Although the autoimmunity against thyroid tissue specific antigens has been studied extensively, so far, the autoantibody responses against common TAAs such as cancer-testis antigens (CTAs), mutated or differentiation antigens have not been comprehensively analyzed in patients with thyroid cancer.
  
  
  OBJECTIVE
  The current study aims to characterize the frequency of autoantibody responses against common TAAs in patients with thyroid cancer and benign thyroid nodules.
  
  
  METHODS
  A phage-displayed antigen microarray comprising 65 TAAs was produced and tested with sera from 53 patients with thyroid cancer, 90 patients with benign thyroid nodules and 96 cancer-free individuals, 100 melanoma, 54 breast cancer and 14 lung cancer patients as controls.
  
  
  RESULTS
  A panel of 6 TAAs was identified that preferentially reacted with sera from patients with thyroid cancer. The top ranked antigen in this panel was GAGE1 eliciting autoantibody response in 6% of patients with thyroid cancer but not with benign nodules, whereas no reactivity to other CTAs was detected in the sera from patients with thyroid cancer.
  
  
  CONCLUSIONS
  Although six TAAs, including one CTA, showed thyroid cancer-associated reactivity, overall, spontaneous humoral immune responses against TAAs are rare in thyroid cancer and their utility for the development of non-invasive assay for the differential diagnosis of thyroid nodules is limited.
authors.affiliation: Latvian Biomedical Research and Study Centre, Riga, Latvia University of Latvia, Riga, Latvia.
authors.forename: A
authors.initials: A
authors.lastname: Abols
authors.affiliation: University of Latvia, Riga, Latvia.
authors.forename: K
authors.initials: K
authors.lastname: Ducena
authors.affiliation: Latvian Biomedical Research and Study Centre, Riga, Latvia.
authors.forename: P
authors.initials: P
authors.lastname: Zayakin
authors.affiliation: Latvian Biomedical Research and Study Centre, Riga, Latvia.
authors.forename: K
authors.initials: K
authors.lastname: Silina
authors.affiliation: Latvian Biomedical Research and Study Centre, Riga, Latvia.
authors.forename: Z
authors.initials: Z
authors.lastname: Kalnina
authors.affiliation: University of Latvia, Riga, Latvia.
authors.forename: L
authors.initials: L
authors.lastname: Sadovska
authors.affiliation: Latvian Oncology Center, Riga Eastern Clinical University Hospital, Riga, Latvia.
authors.forename: J
authors.initials: J
authors.lastname: Tars
authors.affiliation: Pauls Stradins University Hospital, Riga, Latvia.
authors.forename: J
authors.initials: J
authors.lastname: Vilmanis
authors.affiliation: Pauls Stradins University Hospital, Riga, Latvia.
authors.forename: Z
authors.initials: Z
authors.lastname: Narbuts
authors.affiliation: Latvian Oncology Center, Riga Eastern Clinical University Hospital, Riga, Latvia.
authors.forename: J
authors.initials: J
authors.lastname: Eglitis
authors.affiliation: University of Latvia, Riga, Latvia Pauls Stradins University Hospital, Riga, Latvia.
authors.forename: V
authors.initials: V
authors.lastname: Pirags
authors.affiliation: Latvian Biomedical Research and Study Centre, Riga, Latvia.
authors.forename: A
authors.initials: A
authors.lastname: Line
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000097:blood* / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000097:blood / Q000276:immunology; D001943:Breast Neoplasms / Q000097:blood / Q000276:immunology; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000276:immunology; D008297:Male; D008545:Melanoma / Q000097:blood / Q000276:immunology; D008875:Middle Aged; D013964:Thyroid Neoplasms / Q000097:blood* / Q000276:immunology*; D055815:Young Adult
chemical_list: D000951:Antigens, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor
keywords: Phage-displayed antigen microarray; autoantibodies; biomarker; cancer-testis antigens; thyroid cancer; thyroid nodules