title: Transcriptome annotation and marker discovery in white bass (Morone chrysops) and striped bass (Morone saxatilis).
abstract: Striped bass (Morone saxatilis) and white bass (Morone chrysops) are the parental species of the hybrid striped bass, a major U.S. aquaculture species. Currently, genomic resources for striped bass, white bass, and their hybrid lag behind those of other aquaculture species. Current resources consist of a medium-density genetic linkage map and a well-annotated ovarian transcriptome. A well-annotated transcriptome from across striped bass and white bass tissues is needed to advance both broad-based RNA-seq studies of gene expression as well as aid in more targeted studies of important genes and pathways critical for reproductive physiology and immunity. Here, we carried out Illumina-based transcriptome sequencing and annotation in both species utilizing the trinity and trinotate packages. The assembled Moronid reference transcriptomes and identified SSRs and SNPs should advance ongoing studies of reproduction, physiology, and immunology in these species and provide markers for broodstock management and selection.
authors.affiliation: School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Benjamin H
authors.initials: BH
authors.lastname: Beck
authors.forename: S Adam
authors.initials: SA
authors.lastname: Fuller
authors.forename: Eric
authors.initials: E
authors.lastname: Peatman
mesh_terms: D000818:Animals; D001492:Bass / Q000145:classification / Q000235:genetics*; D020451:Contig Mapping; D018895:Microsatellite Repeats; D058977:Molecular Sequence Annotation; D020641:Polymorphism, Single Nucleotide; D013045:Species Specificity; D059467:Transcriptome
keywords: Morone; RNA-seq; SNP; gene assembly