title: Ontogeny of the major xenobiotic-metabolizing enzymes expression and the dietary lipids modulatory effect in the rat dimethylbenz(a)anthracene-induced breast cancer model.
abstract: Breast cancer is the most common malignancy in women worldwide. Environmental factors such as xenobiotic exposure and lifestyle and nutrition play a key role in its etiology. This study was designed to evaluate the age-related changes in the expression of major xenobiotic-metabolizing enzymes (XMEs) in the rat liver and the mammary gland in the dimethylbenz(a)anthracene-induced breast cancer model. The influence of dietary lipids on the ontogeny of XMEs was also evaluated. mRNA and protein levels of phase I (CYP1A1, CYP1A2, and CYP1B1) and phase II (NAD(P)H:quinone acceptor oxidoreductase 1 and GSTP1) enzymes were analyzed, as well as their regulation by AhR and Nrf2, respectively. Results showed differences in the phase I enzymes expression, whereas little changes were obtained in phase II. High corn oil and olive oil diets differentially influenced the expression of age-related changes, suggesting that the different susceptibility to xenobiotic exposure depending upon the age may be modulated by dietary factors.
authors.affiliation: Department of Cell Biology, Physiology and Immunology, Medical Physiology Unit, School of Medicine, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain. Eduard.Escrich@uab.es.
authors.forename: Miguel Ángel
authors.initials: MÁ
authors.lastname: Manzanares
authors.forename: Montserrat
authors.initials: M
authors.lastname: Solanas
authors.forename: Raquel
authors.initials: R
authors.lastname: Moral
authors.forename: Raquel
authors.initials: R
authors.lastname: Escrich
authors.forename: Elena
authors.initials: E
authors.lastname: Vela
authors.forename: Eduard
authors.initials: E
authors.lastname: Escrich
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity*; D000375:Aging / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis*; D002273:Carcinogens / Q000633:toxicity*; D003314:Corn Oil / Q000494:pharmacology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D051549:Glutathione S-Transferase pi / Q000096:biosynthesis*; D015674:Mammary Neoplasms, Animal / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000096:biosynthesis*; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism*; D000069463:Olive Oil; D010938:Plant Oils / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism; D015262:Xenobiotics
chemical_list: D002273:Carcinogens; D051267:NF-E2-Related Factor 2; D009363:Neoplasm Proteins; C495637:Nfe2l2 protein, rat; D000069463:Olive Oil; D010938:Plant Oils; D018336:Receptors, Aryl Hydrocarbon; D015262:Xenobiotics; D015127:9,10-Dimethyl-1,2-benzanthracene; D003314:Corn Oil; D001189:Aryl Hydrocarbon Hydroxylases; D016660:NAD(P)H Dehydrogenase (Quinone); C507703:NQO1 protein, rat; D051549:Glutathione S-Transferase pi; C496558:Gstp1 protein, rat
keywords: Breast Cancer Model; Dietary Lipids; Ontogeny; Polycyclic Aromatic Hydrocarbons (PAHs); Xenobiotic-Metabolizing Enzymes (XMEs)