title: Developmental stage dependent metabolic regulation during meiotic differentiation in budding yeast.
abstract: BACKGROUND
  The meiotic developmental pathway in yeast enables both differentiation of vegetative cells into haploid spores that ensure long-term survival, and recombination of the parental DNA to create genetic diversity. Despite the importance of proper metabolic regulation for the supply of building blocks and energy, little is known about the reprogramming of central metabolic pathways in meiotically differentiating cells during passage through successive developmental stages.
  
  
  RESULTS
  Metabolic regulation during meiotic differentiation in budding yeast was analysed by integrating information on genome-wide transcriptional activity, 26 enzymatic activities in the central metabolism, the dynamics of 67 metabolites, and a metabolic flux analysis at mid-stage meiosis. Analyses of mutants arresting sporulation at defined stages demonstrated that metabolic reprogramming is tightly controlled by the progression through the developmental pathway. The correlation between transcript levels and enzymatic activities in the central metabolism varies significantly in a developmental-stage dependent manner. The complete loss of phosphofructokinase activity at mid-stage meiosis enables a unique setup of the glycolytic pathway which facilitates carbon flux repartitioning into synthesis of spore-wall precursors during the co-assimilation of glycogen and acetate. The need for correct homeostasis of purine nucleotides during the meiotic differentiation was demonstrated by the sporulation defect of the AMP deaminase mutant, amd1, which exhibited hyper-accumulation of ATP accompanied by depletion of guanosine nucleotides.
  
  
  CONCLUSIONS
  Our systems-level analysis shows that reprogramming of the central metabolism during the meiotic differentiation is controlled at different hierarchical levels to meet the metabolic and energetic needs at successive developmental stages.
authors.forename: Thomas
authors.initials: T
authors.lastname: Walther
authors.forename: Fabien
authors.initials: F
authors.lastname: Létisse
authors.forename: Lindsay
authors.initials: L
authors.lastname: Peyriga
authors.forename: Ceren
authors.initials: C
authors.lastname: Alkim
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Liu
authors.forename: Aurélie
authors.initials: A
authors.lastname: Lardenois
authors.forename: Hélène
authors.initials: H
authors.lastname: Martin-Yken
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Portais
authors.forename: Michael
authors.initials: M
authors.lastname: Primig
authors.forename: Jean
authors.initials: J
authors.lastname: François
mesh_terms: D015966:Gene Expression Regulation, Fungal; D008540:Meiosis; D055442:Metabolome; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins