title: Possible involvement of eEF1A in Tomato spotted wilt virus RNA synthesis.
abstract: Tomato spotted wilt virus (TSWV) is a negative-strand RNA virus in the family Bunyaviridae and propagates in both insects and plants. Although TSWV can infect a wide range of plant species, host factors involved in viral RNA synthesis of TSWV in plants have not been characterized. In this report, we demonstrate that the cell-free extract derived from one of the host plants can activate mRNA transcriptional activity of TSWV. Based on activity-guided fractionation of the cell-free extract, we identified eukaryotic elongation factor (eEF) 1A as a possible host factor facilitating TSWV transcription and replication. The RNA synthesis-supporting activity decreased in the presence of an eEF1A inhibitor, suggesting that eEF1A plays an important role in RNA synthesis of TSWV.
authors.affiliation: Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan. Electronic address: a-komoda@mail.ecc.u-tokyo.ac.jp.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Komoda
authors.affiliation: Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ishibashi
authors.affiliation: Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: Kazue
authors.initials: K
authors.lastname: Kawamura-Nagaya
authors.affiliation: Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishikawa
mesh_terms: D002460:Cell Line; D039642:Eukaryotic Initiation Factors / Q000235:genetics / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D010936:Plant Extracts / Q000737:chemistry; D010940:Plant Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis*; D017865:Tospovirus / Q000235:genetics / Q000378:metabolism*
chemical_list: D039642:Eukaryotic Initiation Factors; D010936:Plant Extracts; D010940:Plant Proteins; D012333:RNA, Messenger; D012367:RNA, Viral
keywords: Bunyavirus; Cell-free system; Host factor; Replication; Tomato spotted wilt virus; Transcription; eEF1A