title: Visualization and body distribution of [¹³¹I]-herceptin in nude mice with BT-474 breast carcinoma.
abstract: The study aimed to investigate the bio-distribution and radio-immuno-imaging features of [(131)I]-herceptin in nude mice with BT-474 breast carcinoma. [(131)I]-Herceptin was administrated by tail intravenous injection to the nude mice with BT-474 breast carcinoma. Radiocounting was performed at 4, 12, 24, 48, and 96 h after administration. The activity ratio in the tumor tissue and non-tumor tissue (T/NT) and the radiocounting percentage per gram tissue to the injected dose (%ID/g) were calculated. The nude mice with BT-474 breast carcinoma were also visualized continuously by single photon emission computed tomography at 2, 4, 8, 12, 24, 48, and 96 h after the injection of [(131)I]-herceptin. Nude mice with MDA-MB-231 used as the control group were subjected to the same analyses. Clear tumor images were obtained after the injection of [(131)I]-herceptin in nude mice with BT-474 breast carcinoma. The images were the clearest at 24 h after the injection and remained clear even at 96 h. The T/NT ratio and %ID/g in the tumor tissues of nude mice with BT-474 were both significantly higher than those of the control group (P < 0.01). [(131)I]-Herceptin displays tumors clearly in the nude mice with BT-474 and accumulates well in the tumor tissues.
authors.affiliation: Department of Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Z X
authors.initials: ZX
authors.lastname: Yang
authors.affiliation: Department of Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: H
authors.initials: H
authors.lastname: Cao
authors.affiliation: Department of Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: C G
authors.initials: CG
authors.lastname: Xing
authors.affiliation: Department of Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: S H
authors.initials: SH
authors.lastname: Wei
authors.affiliation: Department of Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China guoqinjiangcn@126.com.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Jiang
authors.affiliation: Department of Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Z L
authors.initials: ZL
authors.lastname: Liu
mesh_terms: D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000493:pharmacokinetics*; D000970:Antineoplastic Agents / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics*; D008325:Mammary Neoplasms, Experimental / Q000175:diagnosis / Q000000981:diagnostic imaging / Q000378:metabolism*; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors; D014018:Tissue Distribution; D015899:Tomography, Emission-Computed, Single-Photon; D014183:Transplantation, Heterologous; D000068878:Trastuzumab
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D000970:Antineoplastic Agents; D007457:Iodine Radioisotopes; D000068878:Trastuzumab