title: Synthesis and characterization of hyaluronic acid/human-like collagen hydrogels.
abstract: Injectable hydrogel plays an important role in soft tissue filling and repair. We report an injectable hydrogel based on hyaluronic acid (HA) and human-like collagen (HLC), both with favorable biocompatibility and biodegradability. These two types of biomacromolecules were crosslinked with 1,4-butanediol diglycidyl ether to form a three-dimensional network. The redundant crosslinker was removed by dialysis and distillation. An HA-based hydrogel prepared by the same method was used as a control. The cytocompatibility was studied with a Cell Counting Kit-8 (CCK-8) test. Carbazole colorimetry was used to analyze the in vitro degradation rate. The histocompatibility was evaluated by hematoxylin and eosin (H&E) staining analysis and immunohistochemical analysis. The CCK-8 assay demonstrated that the HA/HLC hydrogel was less cytotoxic than the HA-based hydrogel and could promote baby hamster kidney cell (BHK) proliferation. The cell adhesion indicated that BHK could grow well on the surface of the materials and maintain good cell viability. The in vitro degradation test showed that the HA/HLC hydrogel had a longer degradation time and an excellent antienzyme ability. In vivo injection showed that there was little inflammatory response to HA/HLC after 1, 2, and 4 weeks. Therefore, the HA/HLC hydrogel is a promising biomaterial for soft tissue filling and repair.
authors.affiliation: Shaanxi Key Laboratory of Degradable Biomedical Materials, Department of Chemical Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Shaanxi Key Laboratory of Degradable Biomedical Materials, Department of Chemical Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China. Electronic address: xiaoxuanma@163.com.
authors.forename: Xiaoxuan
authors.initials: X
authors.lastname: Ma
authors.affiliation: Shaanxi Key Laboratory of Degradable Biomedical Materials, Department of Chemical Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China. Electronic address: fandaidi@nwu.edu.cn.
authors.forename: Daidi
authors.initials: D
authors.lastname: Fan
authors.affiliation: Shaanxi Key Laboratory of Degradable Biomedical Materials, Department of Chemical Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China.
authors.forename: Chenhui
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Shaanxi Key Laboratory of Degradable Biomedical Materials, Department of Chemical Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Deng
authors.affiliation: Shaanxi Key Laboratory of Degradable Biomedical Materials, Department of Chemical Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Hui
authors.affiliation: Shaanxi Key Laboratory of Degradable Biomedical Materials, Department of Chemical Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Ma
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002478:Cells, Cultured; D003094:Collagen / Q000138:chemical synthesis* / Q000737:chemistry; D003124:Colorimetry; D006224:Cricetinae; D006801:Humans; D006820:Hyaluronic Acid / Q000138:chemical synthesis / Q000737:chemistry*; D020100:Hydrogels; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D006820:Hyaluronic Acid; D003094:Collagen
keywords: Biocompatibility; Biodegradability; Human-like collagen; Hyaluronic acid; Hydrogel