title: Isolation, characterization, and cross-transferability of microsatellite markers from the whitebacked planthopper (Sogatella furcifera).
abstract: The whitebacked planthopper Sogatella furcifera (Horváth) (Hemiptera: Delphacidae) is one of the most harmful pests of rice. In this study, 18 polymorphic microsatellite markers were developed from S. furcifera genomic libraries using the fast isolation by amplified fragment length polymorphism of sequences containing repeat protocols. Microsatellite polymorphism was investigated using 32 individuals from one natural population. These 18 simple sequence repeat markers showed a number of alleles that ranged from 3 to 15 and had observed and expected heterozygosities that ranged from 0.094 to 0.871 and from 0.148 to 0.924, respectively. The high cross-species transferability of these markers was evaluated in three other planthopper species: Nilaparvata lugens, N. muiri China, and N. bakeri (Muir). These microsatellite markers will provide powerful tools for population genetic and ecological studies of this pest and its related species in the future.
authors.affiliation: Engineering Research Center of Protection and Utilization for Biological Resources in Minority Regions, South-Central University for Nationalities, Wuhan, Hubei, P.R. China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Engineering Research Center of Protection and Utilization for Biological Resources in Minority Regions, South-Central University for Nationalities, Wuhan, Hubei, P.R. China.
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan Hubei, P.R, China.
authors.forename: G C
authors.initials: GC
authors.lastname: He
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan Hubei, P.R, China.
authors.forename: L L
authors.initials: LL
authors.lastname: Zhu
authors.affiliation: Engineering Research Center of Protection and Utilization for Biological Resources in Minority Regions, South-Central University for Nationalities, Wuhan, Hubei, P.R. China.
authors.forename: R
authors.initials: R
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan Hubei, P.R, China jsl80@163.com.
authors.forename: S L
authors.initials: SL
authors.lastname: Jing
mesh_terms: D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D005787:Gene Frequency; D049750:Genome, Insect; D015698:Genomic Library; D006430:Hemiptera / Q000145:classification / Q000235:genetics*; D006579:Heterozygote; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic