title: Genetic variation of the E-cadherin gene is associated with primary infertility in patients with ovarian endometriosis.
abstract: OBJECTIVE
  To explore the association between the genetic variant of E-cadherin gene and endometriosis-related infertility.
  
  
  METHODS
  Case-control study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Five hundred eighty-nine women with ovarian endometriosis including 127 patients with primary infertility and 589 female controls in northern China.
  
  
  METHODS
  None.
  
  
  METHODS
  Single nucleotide polymorphisms (SNPs) in the promoter region, exons, and the 3' untranslated region of the E-cadherin gene were identified by direct sequencing in patients with ovarian endometriosis and with polymerase chain reaction (PCR). Six candidate SNPs (rs16260, rs28372783, rs1801552, rs1801026, rs8049282, and rs13689) were genotyped by PCR and ligase detection reaction.
  
  
  RESULTS
  The results revealed a significant association of rs8049282 SNP on E-cadherin gene with endometriosis-related infertility. When compared with control women or endometriosis patients who had a history of successful fertility, the CC genotype of rs8049282 may significantly increase the risk of primary infertility in patients with ovarian endometriosis (adjusted odds ratio [OR] = 2.70, 95% confidence interval [CI] 1.45-5.00; OR = 2.54, 95% CI 1.45-4.44, respectively).
  
  
  CONCLUSIONS
  Our results suggested that genetic variants on the E-cadherin gene may be involved in endometriosis-related infertility. The rs8049282 SNP of the E-cadherin gene may be a potential molecular marker for the development of primary infertility in northern Chinese women with ovarian endometriosis.
authors.affiliation: Department of Obstetrics and Gynaecology, Hebei Medical University, Fourth Hospital, Shijiazhuang, People's Republic of China. Electronic address: ksjq62cn@sina.com.
authors.forename: Shan
authors.initials: S
authors.lastname: Kang
authors.affiliation: Department of Molecular Biology, Hebei Medical University, Fourth Hospital, Shijiazhuang, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynaecology, Hebei Medical University, Fourth Hospital, Shijiazhuang, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Molecular Biology, Hebei Medical University, Fourth Hospital, Shijiazhuang, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Molecular Biology, Hebei Medical University, Fourth Hospital, Shijiazhuang, People's Republic of China.
authors.forename: Rong-Miao
authors.initials: RM
authors.lastname: Zhou
authors.affiliation: Department of Obstetrics and Gynaecology, Hebei Medical University, Fourth Hospital, Shijiazhuang, People's Republic of China.
authors.forename: Xi-Wa
authors.initials: XW
authors.lastname: Zhao
mesh_terms: D020413:3' Untranslated Regions; D015703:Antigens, CD; D015820:Cadherins / Q000235:genetics*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002681:China; D004715:Endometriosis / Q000150:complications / Q000175:diagnosis / Q000235:genetics* / Q000503:physiopathology; D005091:Exons; D005260:Female; D005298:Fertility / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005820:Genetic Testing / Q000379:methods; D006785:Hospitals, University; D006801:Humans; D007247:Infertility, Female / Q000175:diagnosis / Q000235:genetics* / Q000503:physiopathology; D016015:Logistic Models; D016017:Odds Ratio; D010049:Ovarian Diseases / Q000150:complications / Q000175:diagnosis / Q000235:genetics* / Q000503:physiopathology; D010641:Phenotype; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012307:Risk Factors
chemical_list: D020413:3' Untranslated Regions; D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins
keywords: E-cadherin gene; endometriosis-related infertility; polymorphism; risk