title: NOTCH1 signaling contributes to cell growth, anti-apoptosis and metastasis in salivary adenoid cystic carcinoma.
abstract: BACKGROUND
  Numerous studies have reported both the tumor-suppressive and oncogenic roles of the Notch pathway, indicating that Notch activity regulates tumor biology in a complex, context-dependent manner. The aim of the present study was to identify the role of NOTCH1 in the cell growth and metastasis of SACC.
  
  
  METHODS
  We analyzed the expression of NOTCH1 in clinical SACC samples using immunohistochemical staining. We silenced the expression of NOTCH1 and overexpressed activated NOTCH1 to elucidate the effects of NOTCH1 on proliferation, migration and invasion. NOTCH1 target genes were validated by real-time PCR.
  
  
  RESULTS
  Our results showed that NOTCH1 was upregulated in SACC tissues when compared with normal tissues, and this upregulation was further enhanced in SACC tissues with metastasis and recurrence when compared with SACC tissues without metastasis. Overexpression of NOTCH1 in SACC cells promoted cell growth, migration and invasion, and knockdown of NOTCH1 inhibited cell proliferation in vitro and tumorigenicity in vivo by inducing cell apoptosis.
  
  
  CONCLUSIONS
  The results of this study suggest that NOTCH1 plays a key role in the cell growth, anti-apoptosis, and metastasis of SACC. NOTCH1 inhibitors might therefore have potential therapeutic applications in treating SACC patients by inhibiting cancer cell growth and metastasis.
authors.affiliation: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China. These authors contributed equally to this work.
authors.forename: Bo-Hua
authors.initials: BH
authors.lastname: Su
authors.affiliation: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China. These authors contributed equally to this work.
authors.forename: Jing
authors.initials: J
authors.lastname: Qu
authors.affiliation: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China. These authors contributed equally to this work.
authors.forename: Min
authors.initials: M
authors.lastname: Song
authors.affiliation: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Huang
authors.affiliation: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China.
authors.forename: Xiao-Meng
authors.initials: XM
authors.lastname: Hu
authors.affiliation: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Pathology, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China.
authors.forename: Lin-Can
authors.initials: LC
authors.lastname: Ding
authors.affiliation: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China.
authors.forename: Lin
authors.initials: L
authors.lastname: She
authors.affiliation: Center of Dental Implant, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Oral and Maxillofacial Surgery, Affiliated First Hospital of Fujian Medical University, Fuzhou, China.
authors.forename: Li-Song
authors.initials: LS
authors.lastname: Lin
authors.affiliation: Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Lin
authors.affiliation: Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China. Molecular Oncology, Moffitt Cancer Center, Tampa, FL, United States.
authors.forename: Da-Li
authors.initials: DL
authors.lastname: Zheng
authors.affiliation: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China.
authors.forename: You-Guang
authors.initials: YG
authors.lastname: Lu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D064593:Heterografts; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D051881:Receptor, Notch1 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D012468:Salivary Gland Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D015398:Signal Transduction; D014162:Transfection; D015854:Up-Regulation
chemical_list: C497761:NOTCH1 protein, human; D034741:RNA, Small Interfering; D051881:Receptor, Notch1