title: Electrochemical and biocompatibility response of newly developed TiZr-based metallic glasses.
abstract: This paper presents systematical investigations, including electrochemical activity, MTT cell assay and in vivo test, on the biocompatibility of three metallic glasses. The electrochemical behaviors and the cell toxicity of two newly developed TiZr-based metallic glasses (MGs), Ti42Zr40Si15Ta3 and Ti40Zr40Si15Cu5, with lower or without unfavorable elements are systematically investigated. Results show that the MGs with low Cu content exhibit a low electrochemical response. Cytotoxicity tests for the MGs and the mediums after the potential state test are evaluated with in vitro MTT assays. The solid specimens and the mediums after the potential state test for the pure Ti, Ti42Zr40Si15Ta3 and Ti40Zr40Si15Cu5 exhibit no significant cytotoxicity in the MTT test, while the tested medium for Ti45Cu35Zr20 MG shows lower cell viability. The inductively coupled plasma-mass spectrometry (ICP-MS) results also indicate that the Cu-rich sample released a significant amount of ions which may be the major factor causing the low viability in the MTT test. The good healing condition and the low C-reactive protein (CRP) index for the implanted New Zealand rabbits in a one-month in vivo test also show the satisfactory short-term biocompatibility of the TiZr-based MGs. The electrochemical measurements, in vitro, and in vivo experiments confirm that the developed TiZr-based MGs with lower Cu content (≦5%) are promising for biomedical purposes.
authors.affiliation: Department of Materials and Optoelectronic Science, Center for Nanoscience and Nanotechnology, National Sun Yat-sen University, Kaohsiung, Taiwan, ROC.
authors.forename: C H
authors.initials: CH
authors.lastname: Huang
authors.affiliation: Department of Mechanical and Electro-Mechanical Engineering, National Sun Yat-sen University, Kaohsiung, Taiwan, ROC.
authors.forename: Y S
authors.initials: YS
authors.lastname: Huang
authors.affiliation: Department of Orthopedics, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC; Orthopedic Research Center, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.
authors.forename: Y S
authors.initials: YS
authors.lastname: Lin
authors.affiliation: Department of Mechanical and Electro-Mechanical Engineering, National Sun Yat-sen University, Kaohsiung, Taiwan, ROC. Electronic address: chehsin@mail.nsysu.edu.tw.
authors.forename: C H
authors.initials: CH
authors.lastname: Lin
authors.affiliation: Department of Materials and Optoelectronic Science, Center for Nanoscience and Nanotechnology, National Sun Yat-sen University, Kaohsiung, Taiwan, ROC. Electronic address: jacobc@faculty.nsysu.edu.tw.
authors.forename: J C
authors.initials: JC
authors.lastname: Huang
authors.affiliation: Department of Orthopedics, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC; Orthopedic Research Center, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC; Department of Orthopedics, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.
authors.forename: C H
authors.initials: CH
authors.lastname: Chen
authors.affiliation: Institute of Materials Science and Engineering, Department of Mechanical Engineering, National Central University, Chung-Li, Taiwan, ROC.
authors.forename: J B
authors.initials: JB
authors.lastname: Li
authors.affiliation: Department of Materials and Optoelectronic Science, Center for Nanoscience and Nanotechnology, National Sun Yat-sen University, Kaohsiung, Taiwan, ROC.
authors.forename: Y H
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Institute of Materials Science and Engineering, Department of Mechanical Engineering, National Central University, Chung-Li, Taiwan, ROC.
authors.forename: J S C
authors.initials: JS
authors.lastname: Jang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D004563:Electrochemistry; D005898:Glass; D008297:Male; D013058:Mass Spectrometry; D011817:Rabbits; D014025:Titanium / Q000737:chemistry*; D015040:Zirconium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D015040:Zirconium; D014025:Titanium
keywords: Biocompatibility; Cytotoxicity; Electrochemical response; Epiphyseal growth plate; Metallic glasses