title: A green salt-leaching technique to produce sericin/PVA/glycerin scaffolds with distinguished characteristics for wound-dressing applications.
abstract: Sericin/PVA/glycerin scaffolds could be fabricated using the freeze-drying technique; they showed good physical and biological properties and can be applied as wound dressings. However, freeze-drying is an energy- and time-consuming process with a high associated cost. In this study, an alternative, solvent-free, energy- and time-saving, low-cost salt-leaching technique is introduced as a green technology to produce sericin/PVA/glycerin scaffolds. We found that sericin/PVA/glycerin scaffolds were successfully fabricated without any crosslinking using a salt-leaching technique. The salt-leached sericin/PVA/glycerin scaffolds had a porous structure with pore interconnectivity. The sericin in the salt-leached scaffolds had a crystallinity that was as high as that of the freeze-dried scaffolds. Compared to the freeze-dried scaffolds with the same composition, the salt-leached sericin/PVA/glycerin scaffolds has larger pores, a lower Young's modulus, and faster rates of biodegradation and sericin release. When cultured with L929 mouse fibroblast cells, a higher number of cells were found in the salt-leached scaffolds. Furthermore, the salt-leached scaffolds were less adhesive to the wound, which would reduce pain upon removal. Therefore, salt-leached sericin/PVA/glycerin scaffolds with distinguished characteristics were introduced as another choice of wound dressing, and their production process was simpler, more energy efficient, and saved time and money compared to the freeze-dried scaffolds.
authors.affiliation: Bioactive Resources for Innovative Clinical Applications Research Unit, Chulalongkorn University and Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, PhayaThai Road, Phatumwan, Bangkok, 10330, Thailand.
authors.forename: Pornanong
authors.initials: P
authors.lastname: Aramwit
authors.forename: Juthamas
authors.initials: J
authors.lastname: Ratanavaraporn
authors.forename: Sanong
authors.initials: S
authors.lastname: Ekgasit
authors.forename: Duangta
authors.initials: D
authors.lastname: Tongsakul
authors.forename: Nipaporn
authors.initials: N
authors.lastname: Bang
mesh_terms: D000818:Animals; D001458:Bandages; D005612:Freeze Drying; D005990:Glycerol / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D047030:Sericins / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D047030:Sericins; D011142:Polyvinyl Alcohol; D005990:Glycerol
keywords: freeze-drying; salt-leaching; scaffold; sericin; wound dressing