title: Efficient voltammetric discrimination of free bilirubin from uric acid and ascorbic acid by a CVD nanographite-based microelectrode.
abstract: We report a novel electrochemical sensor based on nanographite grown on platinum microelectrodes for the determination of bilirubin in the presence of normal concentrations of albumin. The albumin is a protein with an intrinsic ability to bind the bilirubin therefore reducing the concentration of the free electroactive metabolite in human fluids. In addition, the proposed device permits the discrimination of free bilirubin from two interferents, uric acid and ascorbic acid, by the separation of their oxidation peaks in voltammetry. Preliminary measurements in human serum prove that the proposed nanostructured platform can be used to detect bilirubin.
authors.affiliation: Laboratory of Integrated Systems, EPFL - École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. Electronic address: irene.taurino@epfl.ch.
authors.forename: Irene
authors.initials: I
authors.lastname: Taurino
authors.affiliation: Laboratory of Clinical Biochemistry, Antwerp University Hospital, Edegem, Belgium; University of Antwerp, Wilrijk, Belgium.
authors.forename: Viviane
authors.initials: V
authors.lastname: Van Hoof
authors.affiliation: Crystal Growth Facility, EPFL - École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Magrez
authors.affiliation: Laboratory of Physics of Complex Matter, EPFL - École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
authors.forename: László
authors.initials: L
authors.lastname: Forró
authors.affiliation: Laboratory of Integrated Systems, EPFL - École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
authors.forename: Giovanni
authors.initials: G
authors.lastname: De Micheli
authors.affiliation: Laboratory of Integrated Systems, EPFL - École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
authors.forename: Sandro
authors.initials: S
authors.lastname: Carrara
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis* / Q000737:chemistry; D001663:Bilirubin / Q000032:analysis* / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D008839:Microelectrodes; D010084:Oxidation-Reduction; D010984:Platinum / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry; D014527:Uric Acid / Q000032:analysis* / Q000737:chemistry
chemical_list: D012709:Serum Albumin; D014527:Uric Acid; D010984:Platinum; D006108:Graphite; D001205:Ascorbic Acid; D001663:Bilirubin
keywords: Albumin; Ascorbic acid; Bilirubin; CVD nanographite; Electrochemical biosensor; Human serum; Uric acid