title: Direct, rapid, and label-free detection of enzyme-substrate interactions in physiological buffers using CMOS-compatible nanoribbon sensors.
abstract: We demonstrate the versatility of Al2O3-passivated Si nanowire devices ("nanoribbons") in the analysis of enzyme-substrate interactions via the monitoring of pH change. Our approach is shown to be effective through the detection of urea in phosphate buffered saline (PBS), and penicillinase in PBS and urine, at limits of detection of <200 μM and 0.02 units/mL, respectively. The ability to extract accurate enzyme kinetics and the Michaelis-Menten constant (Km) from the acetylcholine-acetylcholinesterase reaction is also demonstrated.
authors.affiliation: Department of Electrical Engineering, Yale University , New Haven, Connecticut 06511, United States.
authors.forename: Luye
authors.initials: L
authors.lastname: Mu
authors.forename: Ilia A
authors.initials: IA
authors.lastname: Droujinine
authors.forename: Nitin K
authors.initials: NK
authors.lastname: Rajan
authors.forename: Sonya D
authors.initials: SD
authors.lastname: Sawtelle
authors.forename: Mark A
authors.initials: MA
authors.lastname: Reed
mesh_terms: D000109:Acetylcholine / Q000737:chemistry; D000110:Acetylcholinesterase / Q000737:chemistry; D000537:Aluminum Oxide / Q000737:chemistry; D002021:Buffers; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D057230:Limit of Detection; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D053770:Nanowires; D010405:Penicillinase / Q000737:chemistry; D010406:Penicillins / Q000737:chemistry; D011485:Protein Binding; D012825:Silicon / Q000737:chemistry*; D012984:Software; D013379:Substrate Specificity
chemical_list: D002021:Buffers; D037742:Nanotubes, Carbon; D010406:Penicillins; D000110:Acetylcholinesterase; D010405:Penicillinase; D000537:Aluminum Oxide; D000109:Acetylcholine; D012825:Silicon
keywords: Nanobiosensors; enzyme activity detection; enzyme kinetics; enzyme−substrate interaction; pH sensing