title: Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse.
abstract: To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression of recombinant 6×his-gVEGF164 protein was induced by 0.5 mM isopropyl thio-β-D-galactoside at 32°C. Recombinant goat VEGF164 (rgVEGF164) was purified and identi ed by western blot using monoclonal anti-his and anti-VEGF antibodies. The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group. Thus, rgVEGF164 increases hair growth in mice.
authors.forename: Wenlei
authors.initials: W
authors.lastname: Bao
authors.forename: Jianxin
authors.initials: J
authors.lastname: Yin
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Guo
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Wang
authors.forename: Dongjun
authors.initials: D
authors.lastname: Liu
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
keywords: Goat; Hair Growth; Recombinant Protein; Vascular Endothelial Growth Factor (VEGF)