title: Production, purification and characterization of bacteriocin from Lactobacillus murinus AU06 and its broad antibacterial spectrum.
abstract: OBJECTIVE
  To study the production, purification and characterization of bacteriocin from Lactobacillus murinus AU06 isolated from marine sediments and its broad spectrum of inhibition against fish pathogens.
  
  
  METHODS
  The selected strain was used in production, purification and characterized of bacteriocin. In addition, purified bacteriocin was tested for its antimicrobial activity against fish pathogens.
  
  
  RESULTS
  In the present study, the bacteriocin production was found to be higher at 35 °C, pH 6.0 and was purified to 4.74 fold with 55. 38 U/mg of specific activity with the yield of 28.92%. The molecular weight of the purified bacteriocin was estimated as 21 kDa. The purified bacteriocin exhibited complete inactivation of antimicrobial activity when treated with proteinase K, pronase, chymotrypsin, trypsin, pepsin and papain. The purified bacteriocin exhibited broad inhibitory spectrum against both Gram positive and negative bacteria.
  
  
  CONCLUSIONS
  It is concluded that the ability of bacteriocin in inhibiting a wide-range of pathogenic bacteria is of potential interest for food safety and may have future applications in food preservative.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Sivaramasamy
authors.initials: S
authors.lastname: Elayaraja
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai-608502, Tamilnadu, India.
authors.forename: Neelamegam
authors.initials: N
authors.lastname: Annamalai
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai-608502, Tamilnadu, India.
authors.forename: Packiyam
authors.initials: P
authors.lastname: Mayavu
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai-608502, Tamilnadu, India.
authors.forename: Thangavel
authors.initials: T
authors.lastname: Balasubramanian
keywords: Antibacterial; Bacteriocin; Lactobacillus murinus; Pathogen; Purification