title: Short-term diet and moderate exercise in young overweight men modulate cardiocyte and hepatocarcinoma survival by oxidative stress.
abstract: The present study was designed to evaluate the effects of diet lifestyle on extending lifespan and reducing liver cancer risk. Young overweight men (n = 20), without metabolic syndrome, were placed in a 3-week residential program on a low-fat diet and moderate aerobic exercise. In each subject, pre- and postintervention fasting blood were collected for evaluating levels of serum lipids, and oxidative stress markers. Using subject sera and cardiomyocyte (H9C2) culture systems, we measured heat shock protein 27 and 90 expression, lipid accumulation, and oxidative stress marker levels. After 3-weeks of diet, significant reductions (P < 0.05) in body mass index, serum lipids and lipid ratios, and oxidative markers were recorded. In vitro, we observed that the addition of postintervention sera increased H9C2 cell number and reduced HSP27 and 90 expression, mitochondrial superoxide anion, and lipid accumulation with a parallel increase in nitric oxide (NO) production (all P < 0.01). At the same time, postintervention sera decreased human liver hepatocellular carcinoma cell line (HepG-2) proliferation, lipid accumulation, oxidative stress, and extracellular-signal-regulated kinases (ERK1/2) activity. Lifestyle modification in young overweight men, without metabolic syndrome, could ameliorate cardiocyte survival and reduce hepatocellular carcinoma cell proliferation.
authors.affiliation: Department of Experimental Medicine, Second University of Naples, 80138 Naples, Italy.
authors.forename: Marcellino
authors.identifier: 0000-0002-7184-218X
authors.initials: M
authors.lastname: Monda
authors.affiliation: Department of Experimental Medicine, Second University of Naples, 80138 Naples, Italy.
authors.forename: Giovanni
authors.identifier: 0000-0002-0011-867X
authors.initials: G
authors.lastname: Messina
authors.affiliation: Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, 80138 Naples, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Scognamiglio
authors.affiliation: Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, 80138 Naples, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Lombardi
authors.affiliation: Department of Veterinary Medicine and Animal Reproduction, Federico II University, 80138 Naples, Italy.
authors.forename: Giuseppe A
authors.initials: GA
authors.lastname: Martin
authors.affiliation: Unit of General and Geriatric Surgery, School of Medicine, Second University of Naples, 80137 Naples, Italy.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Sperlongano
authors.affiliation: Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, 80138 Naples, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Porcelli
authors.affiliation: Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, 80138 Naples, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Caraglia
authors.affiliation: Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, 80138 Naples, Italy.
authors.forename: Paola
authors.identifier: 0000-0001-5096-7488
authors.initials: P
authors.lastname: Stiuso
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D018752:Diet, Fat-Restricted; D015444:Exercise; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D008055:Lipids / Q000097:blood; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D050177:Overweight; D018384:Oxidative Stress; D051381:Rats; D013481:Superoxides / Q000378:metabolism; D013997:Time Factors; D055815:Young Adult
chemical_list: D003470:Culture Media; D055551:HSP27 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D008055:Lipids; D013481:Superoxides; D009569:Nitric Oxide; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3