title: Correlation between PARP-1 Val762Ala polymorphism and the risk of lung cancer in a Chinese population.
abstract: The aim of this study was to investigate the relationship of the PARP-1 Val762Ala (rs1136410 T>C) polymorphism and the risk of lung cancer. A population-based case-control study of 373 lung cancer patients and 360 healthy control subjects (individually matched on age and gender) in a Chinese population was conducted. Genomic DNA was extracted by the phenol-chloroform method from the peripheral blood. PARP-1 Val762Ala polymorphism was identified using polymerase chain reaction-restriction fragments length polymorphism technique. After adjusting for age, tobacco smoking, gender, smoking index, and drinking status, logistic regression analysis demonstrated that CC genotype in PARP-1 Val762Ala polymorphism had an increased risk of lung cancer compared with TT genotype (OR = 1.59, 95 % CI = 1.03 ~ 2.50, P = 0.048), a statistically difference that still existed when merging CC and TC genotypes (OR = 1.56, 95 % CI = 1.03 ~ 2.44, P = 0.042). However, no obvious difference was found between TT and TC (OR = 1.54, 95 % CI = 0.96 ~ 2.44, P = 0.073). Subgroup analysis by histological type indicated that adenocarcinoma patients had higher frequencies of CC or TC+CC genotypes than healthy controls (CC: OR = 1.85, 95 % CI = 1.12 ~ 3.03, P = 0.015; TC+CC: OR = 1.67, 95 % CI = 1.06 ~ 2.63, P = 0.027, respectively), but no statistically significant difference within each genotype in squamous cell carcinoma or small cell lung cancer (all P > 0.05). Our findings support the view that PARP-1 Val762Ala polymorphism may contribute to an increased risk of lung cancer in the Chinese population, especially for adenocarcinoma.
authors.affiliation: Department of Medical Oncology, The First Hospital of China Medical University, Nanjing South Street No. 155, Heping District, Shenyang, 110001, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yu
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Liu
authors.forename: Jing-Dong
authors.initials: JD
authors.lastname: Zhang
authors.forename: Xiu-Juan
authors.initials: XJ
authors.lastname: Qu
authors.forename: Bo
authors.initials: B
authors.lastname: Jin
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D019943:Amino Acid Substitution; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D055752:Small Cell Lung Carcinoma / Q000235:genetics*
chemical_list: C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases