title: Ananas comosus L. Leaf Phenols and p-Coumaric Acid Regulate Liver Fat Metabolism by Upregulating CPT-1 Expression.
abstract: In this study, we aimed to investigate the effect and action mechanisms of pineapple leaf phenols (PLPs) on liver fat metabolism in high-fat diet-fed mice. Results show that PLP significantly reduced abdominal fat and liver lipid accumulation in high-fat diet-fed mice. The effects of PLP were comparable with those of FB. Furthermore, at the protein level, PLP upregulated the expression of carnitine palmitoyltransferase 1 (CPT-1), whereas FB had no effects on CPT-1 compared with the HFD controls. Regarding mRNA expression, PLP mainly promoted the expression of CPT-1, PGC1a, UCP-1, and AMPK in the mitochondria, whereas FB mostly enhanced the expression of Ech1, Acox1, Acaa1, and Ehhadh in peroxisomes. PLP seemed to enhance fat metabolism in the mitochondria, whereas FB mainly exerted the effect in peroxisomes. In addition, p-coumaric acid (CA), one of the main components from PLP, significantly inhibited fat accumulation in oleic acid-induced HepG2 cells. CA also significantly upregulated CPT-1 mRNA and protein expressions in HepG2 cells. We, firstly, found that PLP enhanced liver fat metabolism by upregulating CPT-1 expression in the mitochondria and might be promising in treatment of fatty liver diseases as alternative natural products. CA may be one of the active components of PLP.
authors.affiliation: Division of Life Science & Health, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
authors.forename: Weidong
authors.identifier: 0000-0002-0034-8707
authors.initials: W
authors.lastname: Xie
authors.affiliation: Zhu Jiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Shaobo
authors.identifier: 0000-0003-0005-8651
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Protein Science Laboratory of the Ministry of Education, Laboratory of Pharmaceutical Science, School of Life Science, School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Lei
authors.affiliation: Division of Life Science & Health, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Ouyang
authors.affiliation: Protein Science Laboratory of the Ministry of Education, Laboratory of Pharmaceutical Science, School of Life Science, School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Du