title: [Ginger extracts improve renal injury of high fructose-fed SD rats by inhibiting the expression of proinflammatory cytokines].
abstract: OBJECTIVE
  To investigated the effects of ginger extracts on renal injury in high fructose-fed SD rats and the possible underlying mechanism.
  
  
  METHODS
  Twenty rats were randomly divided into control group, fructose group, 20 and 50 mg/kg ginger extract groups, 5 rats in each group. The general situation and histopathological changes in the kidney of rats were observed. The expressions of monocyte chemokine protein 1 (MCP-1), tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) mRNA were detected by real-time quantitative PCR (qRT-PCR); the levels of MCP-1, TNF-α and IL-6 in supernatant of renal tissue homogenates were assessed by ELISA.
  
  
  RESULTS
  Compared to the control group, the kidney mass and the ratio of kidney-to-body mass significantly decreased in fructose group, accompanied by renal tubular injury. Ginger extracts at 50 mg/kg evidently improved renal tubular injury, suppressed the expressions of renal MCP-1, TNF-α and IL-6 mRNA (P<0.05) and decreased the levels of MCP-1 (P<0.05), TNF-α (P<0.01), IL-6 (P<0.05) in kidney tissue homogenates.
  
  
  CONCLUSIONS
  The supplement of ginger extracts can improve fructose-induced renal injury, which may be associated with the suppression on the expression of proinflammatory cytokines.
authors.affiliation: Laboratory of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.affiliation: Laboratory of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Jiang
authors.affiliation: Laboratory of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Lin
mesh_terms: D000818:Animals; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005632:Fructose; D005786:Gene Expression Regulation / Q000187:drug effects*; D020939:Zingiber officinale / Q000737:chemistry*; D018836:Inflammation Mediators / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced / Q000235:genetics / Q000517:prevention & control*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D016207:Cytokines; D018836:Inflammation Mediators; D015850:Interleukin-6; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D005632:Fructose