title: Development of microsatellite markers for the coastal shrub Scaevola taccada (Goodeniaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed for the coastal shrub species Scaevola taccada to estimate the population genetic structure, which may reflect different seed dispersal patterns. •
  
  
  RESULTS
  Thirteen microsatellite primer sets were developed for S. taccada using 454 pyrosequencing. The primer sets were tested on 64 individuals sampled from two populations in Japan. Fragments were amplified using the primers, with one to 10 alleles per locus, and the expected heterozygosity ranged from 0.00 to 0.85. •
  
  
  CONCLUSIONS
  These results indicate the utility of markers in S. taccada for broad estimations of the population genetic structure of this species.
authors.affiliation: Laboratory of Forest Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Haruko
authors.initials: H
authors.lastname: Ando
authors.affiliation: Department of Life Science, Rikkyo University, Tokyo 171-8501, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Emura
authors.affiliation: Faculty of Science, University of the Ryukyus, Okinawa 903-0213, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Denda
authors.affiliation: Laboratory of Forest Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Nakahama
authors.affiliation: Wildlife Research Center, Kyoto University, Kyoto 606-8203, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Inoue-Murayama
authors.affiliation: Laboratory of Forest Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Isagi
keywords: 454 sequencing; Goodeniaceae; Scaevola taccada; genetic structure; seed dispersal