title: [Ectopic expression of duck albumin down-regulates the expressions of IFN-β and myxovirus resistance 1 mRNA in DF-1 cells].
abstract: OBJECTIVE
  To investigate the impact of duck albumin (ALB) gene on the mRNA expression levels of interferon β (IFN-β) and myxovirus resistance-1 (Mx1).
  
  
  METHODS
  The duck ALB gene was subcloned into pEGFP-C1 eukaryotic expression vector, and then the pEGFP-C1-ALB was transiently transfected into chick fibroblast DF-1 by Lipofectamine(TM) 2000. Twenty-four hours later, real-time quantitative PCR was applied to detect the dynamic change of IFN-β and Mx1 mRNA expressions under the stimulation of polyinosinic polycytidylic acid (PolyI:C).
  
  
  RESULTS
  The pEGFP-C1-ALB was constructed successfully, and transfected into DF-1 effectively. The expression levels of IFN-β and Mx1 gene in cells transfected with pEGFP-C1-ALB were significantly lower than those transfected with pEGFP-C1 after 12-hour stimulation of PolyI:C (P<0.05 or P<0.01).
  
  
  CONCLUSIONS
  The expression levels of IFN-β and Mx1 mRNA were down-regulated by over-expression of duck ALB gene.
authors.affiliation: Animal Science and Technology College, Yangzhou University, Yangzhou 225009, China.
authors.forename: Yiyu
authors.initials: Y
authors.lastname: Tong
authors.affiliation: Animal Science and Technology College, Yangzhou University, Yangzhou 225009, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Animal Science and Technology College, Yangzhou University, Yangzhou 225009, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Animal Science and Technology College, Yangzhou University, Yangzhou 225009, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Animal Science and Technology College, Jiangxi Agriculture University, Nanchang 330045, China.
authors.forename: Qinming
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Animal Science and Technology College, Yangzhou University, Yangzhou 225009, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Animal Science and Technology College, Yangzhou University, Yangzhou 225009, China.
authors.forename: Zhengyang
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Animal Science and Technology College, Yangzhou University, Yangzhou 225009, China.
authors.forename: Guohong
authors.initials: G
authors.lastname: Chen
mesh_terms: D000418:Albumins / Q000235:genetics*; D000818:Animals; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D002645:Chickens; D015536:Down-Regulation / Q000235:genetics; D004372:Ducks; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D016899:Interferon-beta / Q000235:genetics*; D008856:Microscopy, Fluorescence; D064547:Myxovirus Resistance Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000418:Albumins; D030161:Avian Proteins; D064547:Myxovirus Resistance Proteins; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins; D016899:Interferon-beta