title: Transfection of the glial cell line-derived neurotrophic factor gene promotes neuronal differentiation.
abstract: Glial cell line-derived neurotrophic factor recombinant adenovirus vector-transfected bone marrow mesenchymal stem cells were induced to differentiate into neuron-like cells using inductive medium containing retinoic acid and epidermal growth factor. Cell viability, microtubule-associated protein 2-positive cell ratio, and the expression levels of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43 protein in the supernatant were significantly higher in glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells compared with empty virus plasmid-transfected bone marrow mesenchymal stem cells. Furthermore, microtubule-associated protein 2, glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43 mRNA levels in cell pellets were statistically higher in glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells compared with empty virus plasmid-transfected bone marrow mesenchymal stem cells. These results suggest that glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells have a higher rate of induction into neuron-like cells, and this enhanced differentiation into neuron-like cells may be associated with up-regulated expression of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43.
authors.affiliation: Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan Province, China ; Department of Anatomy, Luzhou Medical College, Luzhou, Sichuan Province, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Du
authors.affiliation: Research Center for Preclinical Medicine, Luzhou Medical College, Luzhou, Sichuan Province, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Gao
authors.affiliation: Research Center for Preclinical Medicine, Luzhou Medical College, Luzhou, Sichuan Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Deng
authors.affiliation: Department of Anatomy, Luzhou Medical College, Luzhou, Sichuan Province, China.
authors.forename: Nengbin
authors.initials: N
authors.lastname: Chang
authors.affiliation: Department of Anatomy, Luzhou Medical College, Luzhou, Sichuan Province, China.
authors.forename: Huailin
authors.initials: H
authors.lastname: Xiong
authors.affiliation: Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
keywords: bone marrow mesenchymal stem cells; cell differentiation; epidermal growth factor; glial cell line-derived neurotrophic factor; growth-associated protein-43; nerve growth factor; nerve regeneration; neural regeneration; neuron-like cells; recombinant adenovirus vector; retinoic acid; transfection