title: Pharmacokinetics and tissue distribution study of tanshinone IIA after oral administration of Bushen Huoxue Qubi granules to rats with blood stasis syndrome.
abstract: BACKGROUND
  Bushen Huoxue Qubi (BHQ) granules, a traditional Chinese medicine preparation, has been clinically used for the treatment of the blood stasis syndrome.
  
  
  OBJECTIVE
  The main objective is to investigate whether the diseased condition would alter the pharmacokinetics and tissue distribution of tanshinone IIA in BHQ, which was given orally to the acute blood stasis rats.
  
  
  METHODS
  The main bioactive constituent in BHQ, tanshinone IIA, was measured in the plasma and tissues of animals by the high performance liquid chromatography with ultraviolet detection. The analysis was successfully performed on an Agilent TC-C18 column (250 × 4.6 mm I.D., 5 μm) protected with a  Octadecylsilane (ODS) guard column (10 × 4.6 mm I.D., 5 μm). The mobile phase was aqueous solution (A) (containing 0.40% aqueous acetic acid) and acetonitrile (B). The conditions of the solvent gradient elution were 35-40% (B) in 0-15 min, 40-42% (B) in 15-18 min and 42-70% (B) in 18-30 min at a flow rate of 1.0 mL/min. Detection was conducted with wavelength of 270 nm at 30°C.
  
  
  RESULTS
  Good linearity relationships were found (r (2)> 0.9955) over the investigated concentration range for bio-samples. Blood stasis was associated with significantly higher area under the concentration-time curve (AUC), the maximum plasma concentration (Cmax) and biological half-life (t1/2), lower total body clearance (CL) and apparent volume of distribution (Vd) of tanshinone IIA in plasma and higher AUC0-t of tanshinone IIA in the analyzed tissues of rats treated with BHQ.
  
  
  CONCLUSIONS
  Blood stasis could alter pharmacokinetics and tissue distribution of tanshinone IIA in BHQ.
authors.affiliation: Department of Chemistry, Key Laboratory of Modernization of Chinese Medicine, Changsha, China ; Department of Biotechnology, Laboratory of Biotechnology and Engineering, School of Life Science and Technology, Central South University of Forestry and Technology, Changsha, China.
authors.forename: Yuanqing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Key Laboratory of Modernization of Chinese Medicine, Changsha, China.
authors.forename: Jianye
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Chemistry, Key Laboratory of Modernization of Chinese Medicine, Changsha, China.
authors.forename: Shunxiang
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Diagnosis, Diagnostic Institute of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Cai
authors.affiliation: Department of Chemistry, TCM and Ethnomedicine Innovation and Development Laboratory, School of Pharmacy, Changsha, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Key Laboratory of Modernization of Chinese Medicine, Changsha, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Luo
authors.affiliation: Department of Chemistry, Key Laboratory of Modernization of Chinese Medicine, Changsha, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Huang
authors.affiliation: Department of Rheumatism and Immunology, Xiangya Second Hospital of Central South University, Changsha, China.
authors.forename: Jiesheng
authors.initials: J
authors.lastname: Gao
keywords: Blood stasis; Bushen Huoxue Qubi granules; high performance liquid chromatography; pharmacokinetics; tanshinone IIA; tissue distribution