title: Effect of dehydration methods on retention of carotenoids, tocopherols, ascorbic acid and antioxidant activity in Moringa oleifera leaves and preparation of a RTE product.
abstract: Fresh leaves of M. oleifera plants were analysed for nutritionally important phytoconstituents and feasible commercially used dehydration method were evaluated to preserve these in dehydrated leaves. Trans-lutein, trans-β-carotene and trans-zeaxanthin were found as the major carotenoids in fresh leaves, accounting for 36.9, 18.2 and 5.5 mg/100 g FW, respectively. Similarly, high amounts of ascorbic acid, α-tocopherol and total phenolic content (271.0, 36.9 and 512.0 mg/100 g FW), respectively were recorded in leaves. α-tocopherol was the most stable vitamin under all drying conditions (86.4 % retention during oven drying), compare to other studied phytoconstituents. Cabinet tray drying was found as efficient as lyophilisation to retain maximum content of total carotenoids (60.1 %), trans-β-carotene (90.1 %), 13-cis-lutein (93.2 %), and DPPH activity, however, lutein (51.3 %) and ascorbic acid (97.8 %) was best preserved by lyophilisation. During dehydration, significant trans to cis isomerization of β-carotene and lutein was also recorded. A ready to eat (RTE) chutney powder (adjunct) was developed from dehydrated leaves. The product was evaluated using Quantitative Descriptive Analysis and was accepted with a high overall quality score. The present investigation explores the nutritional potential of M. oleifera leaves and suitable methods of drying that could be useful for processed food formulation.
authors.affiliation: Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysore, 570 020 India.
authors.forename: R K
authors.initials: RK
authors.lastname: Saini
authors.affiliation: Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysore, 570 020 India.
authors.forename: N P
authors.initials: NP
authors.lastname: Shetty
authors.affiliation: Sensory Science Department, CSIR-Central Food Technological Research Institute, Mysore, 570 020 India.
authors.forename: Maya
authors.initials: M
authors.lastname: Prakash
authors.affiliation: Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysore, 570 020 India.
authors.forename: P
authors.initials: P
authors.lastname: Giridhar
keywords: Antioxidant activity; Dehydration; Drying; Moringa oleifera; Nutrient composition