title: Development and characterization of 32 microsatellite loci in Genipa americana (Rubiaceae).
abstract: OBJECTIVE
  Microsatellite primers were developed for the tree species Genipa americana (Rubiaceae) for further population genetic studies. •
  
  
  RESULTS
  We identified 144 clones containing 65 repeat motifs from a genomic library enriched for (CT)8 and (GT)8 motifs. Primer pairs were developed for 32 microsatellite loci and validated in 40 individuals of two natural G. americana populations. Seventeen loci were polymorphic, revealing from three to seven alleles per locus. The observed and expected heterozygosities ranged from 0.24 to 1.00 and from 0.22 to 0.78, respectively. •
  
  
  CONCLUSIONS
  The 17 primers identified as polymorphic loci are suitable to study the genetic diversity and structure, mating system, and gene flow in G. americana.
authors.affiliation: Faculdade de Engenharia de Ilha Solteira, Universidade Estadual Paulista (UNESP), Av. Brasil Centro 56, CP 31, Ilha Solteira, São Paulo 15385-000, Brazil.
authors.forename: Ricardo O
authors.initials: RO
authors.lastname: Manoel
authors.affiliation: Instituto Florestal de São Paulo, CP 1322, São Paulo, São Paulo 01059-970, Brazil.
authors.forename: Miguel L M
authors.initials: ML
authors.lastname: Freitas
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas (UNICAMP), CP 6010, Campinas, São Paulo 13083-970, Brazil.
authors.forename: Mariana A
authors.initials: MA
authors.lastname: Barreto
authors.affiliation: Faculdade de Engenharia de Ilha Solteira, Universidade Estadual Paulista (UNESP), Av. Brasil Centro 56, CP 31, Ilha Solteira, São Paulo 15385-000, Brazil.
authors.forename: Mário L T
authors.initials: ML
authors.lastname: Moraes
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas (UNICAMP), CP 6010, Campinas, São Paulo 13083-970, Brazil.
authors.forename: Anete P
authors.initials: AP
authors.lastname: Souza
authors.affiliation: Instituto Florestal de São Paulo, CP 1322, São Paulo, São Paulo 01059-970, Brazil.
authors.forename: Alexandre M
authors.initials: AM
authors.lastname: Sebbenn
keywords: Genipa americana; Rubiaceae; conservation genetics; microsatellite markers; population genetics; tree species