title: Effects of polyphenolic grape extract on the oxidative status of muscle and endothelial cells.
abstract: A grape pomace extract enhanced antioxidant mechanisms in muscle and endothelial cells both in the absence and in the presence of oxidative stress-induced agent tert-butyl hydroperoxide (tBHP). In particular, muscle (C2C12) and endothelial (EA.hy926) cells were treated with the extract at noncytotoxic concentrations for 24 h, and the oxidative stress markers, total reactive oxygen species (ROS), glutathione (GSH), thiobarbituric reactive substances (TBARS), and protein carbonyl levels were assessed. The results showed that the grape extract treatment reduced significantly ROS, TBARS, and protein carbonyl levels and increased GSH in C2C12 cells, while it increased GSH and decreased protein carbonyl levels in EA.hy926 cells. In the presence of tBHP, the grape extract treatment in C2C12 cells reduced significantly ROS, TBARS, and protein carbonyls and increased GSH compared with tBHP alone treatment, while, in EA.hy926 cells, the extract decreased significantly TBARS and protein carbonyls but increased GSH. The antioxidant potency of the extract was different between muscle and endothelial cells suggesting that the antioxidant activity depends on cell type. Moreover, the antioxidant activity of the grape extract, in both cell lines, exerted, at least in part, through increase in GSH levels. The present work is the first to report the effects of grape extract shown for skeletal muscle cells.
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
authors.forename: N
authors.initials: N
authors.lastname: Goutzourelas
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
authors.forename: D
authors.initials: D
authors.lastname: Stagos
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
authors.forename: N
authors.initials: N
authors.lastname: Demertzis
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
authors.forename: P
authors.initials: P
authors.lastname: Mavridou
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
authors.forename: H
authors.initials: H
authors.lastname: Karterolioti
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
authors.forename: S
authors.initials: S
authors.lastname: Georgadakis
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Kerasioti
authors.affiliation: Division of Pharmacognosy and Natural Products Chemistry, School of Pharmacy, University of Athens, Panepistimiopolis Zografou, Athens, Greece.
authors.forename: N
authors.initials: N
authors.lastname: Aligiannis
authors.affiliation: Division of Pharmacognosy and Natural Products Chemistry, School of Pharmacy, University of Athens, Panepistimiopolis Zografou, Athens, Greece.
authors.forename: L
authors.initials: L
authors.lastname: Skaltsounis
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Statiri
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Tsioutsiouliti
authors.affiliation: Department of Forensic Sciences and Toxicology, Medical School, University of Crete, Heraklion, Greece.
authors.forename: A M
authors.initials: AM
authors.lastname: Tsatsakis
authors.affiliation: Harvard School of Public Health, Boston, MA, USA Spherix Consulting, Inc., Bethesda, MD, USA.
authors.forename: A W
authors.initials: AW
authors.lastname: Hayes
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece dkouret@uth.gr.
authors.forename: D
authors.initials: D
authors.lastname: Kouretas
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D051379:Mice; D032342:Muscle Cells / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D027843:Vitis
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D005978:Glutathione
keywords: Grape extract; endothelial cells; muscle cells; oxidative stress