title: Synthesis of Oligonucleotides Containing 4,5',8-Trimethylpsoralen at the 2'-O Position and Their Cross-Linking Properties with RNAs.
abstract: 4,5',8-Trimethylpsoralen-conjugated oligonucleotides have been used in the study of photo-cross-linking with target oligonucleotides and in the field of the photodynamic therapy. This unit describes synthetic procedures for oligonucleotides using 2'-O-methylphosphoramidite units and an adenosine phosphoramidite unit containing a 4,5',8-trimethylpsoralen derivative attached at the 2' position of an adenosine sugar moiety via an ethoxymethylene linkage. Procedures for obtaining the photo-cross-linking efficiency of 2'-O-methyloligonucleotides containing a 4,5',8-trimethylpsoralen derivative with a target oligonucleotide under UV irradiation conditions are also described, together with the procedure for preparation of (32)P-radiolabeled RNA.
authors.affiliation: Kyoto Institute of Technology, Graduate School of Science and Technology, Department of Biomolecular Engineering, Matsugasaki, Sakyo-ku, Kyoto, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Kobori
authors.forename: Asako
authors.initials: A
authors.lastname: Yamayoshi
authors.forename: Akira
authors.initials: A
authors.lastname: Murakami
mesh_terms: D003432:Cross-Linking Reagents / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D014307:Trioxsalen / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D003432:Cross-Linking Reagents; D009841:Oligonucleotides; D012313:RNA; D014307:Trioxsalen
keywords: 4,5′,8-trimethylpsoralen; antisense; cross-link