title: OsAAP6 functions as an important regulator of grain protein content and nutritional quality in rice.
abstract: Grains from cereals contribute an important source of protein to human food, and grain protein content (GPC) is an important determinant of nutritional quality in cereals. Here we show that the quantitative trait locus (QTL) qPC1 in rice controls GPC by regulating the synthesis and accumulation of glutelins, prolamins, globulins, albumins and starch. qPC1 encodes a putative amino acid transporter OsAAP6, which functions as a positive regulator of GPC in rice, such that higher expression of OsAAP6 is correlated with higher GPC. OsAAP6 greatly enhances root absorption of a range of amino acids and has effects on the distribution of various amino acids. Two common variations in the potential cis-regulatory elements of the OsAAP6 5'-untranslated region seem to be associated with GPC diversity mainly in indica cultivars. Our results represent the first step toward unravelling the mechanism of regulation underlying natural variation of GPC in rice.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Peng
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
authors.forename: Huili
authors.initials: H
authors.lastname: Kong
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
authors.forename: Yibo
authors.initials: Y
authors.lastname: Li
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
authors.forename: Lingqiang
authors.initials: L
authors.lastname: Wang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhong
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Sun
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
authors.forename: Guanjun
authors.initials: G
authors.lastname: Gao
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
authors.forename: Qinglu
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Shanghai Agrobiological Gene Center, Shanghai 201106, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Luo
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
authors.forename: Gongwei
authors.initials: G
authors.lastname: Wang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
authors.forename: Weibo
authors.initials: W
authors.lastname: Xie
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
authors.forename: Junxiao
authors.initials: J
authors.lastname: Chen
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Yao
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Peng
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Lei
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
authors.forename: Xingmin
authors.initials: X
authors.lastname: Lian
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Xiao
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
authors.forename: Caiguo
authors.initials: C
authors.lastname: Xu
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
authors.forename: Xianghua
authors.initials: X
authors.lastname: Li
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: He
mesh_terms: D000418:Albumins / Q000096:biosynthesis / Q000378:metabolism; D026905:Amino Acid Transport Systems / Q000235:genetics*; D000596:Amino Acids / Q000378:metabolism; D001483:Base Sequence; D005916:Globulins / Q000096:biosynthesis / Q000378:metabolism; D005983:Glutens / Q000096:biosynthesis / Q000378:metabolism; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots / Q000378:metabolism; D055315:Prolamins / Q000096:biosynthesis / Q000378:metabolism; D040641:Quantitative Trait Loci; D013213:Starch / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D000418:Albumins; D026905:Amino Acid Transport Systems; D000596:Amino Acids; D005916:Globulins; D010940:Plant Proteins; D055315:Prolamins; D005983:Glutens; D013213:Starch