title: Development of microsatellite markers for the neotropical vine Dalechampia scandens (Euphorbiaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed to assess polymorphism and level of genetic diversity in four Mexican populations of the neotropical vine Dalechampia scandens (Euphorbiaceae). •
  
  
  RESULTS
  Thirty-seven microsatellite markers representing bi-, tri-, tetra-, and pentanucleotide microsatellite repeats were developed. In total, 166 alleles were identified across 54 individuals. The number of alleles varied from one to 11 with an average of 4.49 alleles per locus. All loci except one were highly polymorphic between populations, whereas considerably less variation was detected within populations for most loci. The average observed and expected heterozygosities across study populations ranged from 0 to 0.63 and 0 to 0.59, respectively, for individual loci, and a deviation from Hardy-Weinberg equilibrium was observed for most loci. •
  
  
  CONCLUSIONS
  The developed markers may be useful for studying genetic structure, parentage analysis, mapping, phylogeography, and cross-amplification in other closely related species of Dalechampia.
authors.affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Falahati-Anbaran
authors.affiliation: Museum of Natural History and Archaeology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
authors.forename: Hans K
authors.initials: HK
authors.lastname: Stenøien
authors.affiliation: Centre for Biodiversity Dynamics, Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
authors.forename: Christophe
authors.initials: C
authors.lastname: Pélabon
authors.affiliation: Centre for Biodiversity Dynamics, Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
authors.forename: Geir H
authors.initials: GH
authors.lastname: Bolstad
authors.affiliation: Plant Biology and Ecology Department, University of Seville, 41080 Seville, Spain ; School of Biological Sciences, University of Portsmouth, Portsmouth PO1 2DY, United Kingdom.
authors.forename: Rocio
authors.initials: R
authors.lastname: Perez-Barrales
authors.affiliation: Centre for Ecological and Evolutionary Synthesis, Department of Biology, University of Oslo, P.O. Box 1066, N-0316 Oslo, Norway.
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Hansen
authors.affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway ; School of Biological Sciences, University of Portsmouth, Portsmouth PO1 2DY, United Kingdom ; Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, Alaska 99775 USA.
authors.forename: W Scott
authors.initials: WS
authors.lastname: Armbruster
keywords: Dalechampia scandens; Euphorbiaceae; genetic diversity; microsatellite loci