title: Fimbriae and lipopolysaccharides are necessary for co-aggregation between Lactobacilli and Escherichia coli.
abstract: Cells of Lactobacilli co-aggregated with Escherichia coli K-12 cells to form co-aggregates under mixed-culture conditions at 37 °C for 24 h. Co-aggregation was inhibited by sodium dodecyl sulfate but not by protease. E. coli deletion mutants of fimbriae formation and lipopolysaccharide (LPS) formation did not co-aggregate with Lactobacilli. These results showed that fimbriae and LPS are necessary for co-aggregation between Lactobacilli and E. coli.
authors.affiliation: a Department of Materials Science and Chemical Engineering , Kitakyushu National College of Technology , Kitakyushu , Japan.
authors.forename: Kouhei
authors.initials: K
authors.lastname: Mizuno
authors.forename: Soichi
authors.initials: S
authors.lastname: Furukawa
authors.forename: Yumi
authors.initials: Y
authors.lastname: Usui
authors.forename: Madoka
authors.initials: M
authors.lastname: Ishiba
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Ogihara
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Morinaga
mesh_terms: D002449:Cell Aggregation / Q000187:drug effects / Q000235:genetics*; D018920:Coculture Techniques; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism*; D007778:Lactobacillus / Q000166:cytology / Q000235:genetics; D008070:Lipopolysaccharides / Q000235:genetics / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
chemical_list: D008070:Lipopolysaccharides; D036781:Fimbriae Proteins; D012967:Sodium Dodecyl Sulfate
keywords: Escherichia coli; Lactobacillus; co-aggregation; fimbriae; lipopolysaccharide