title: Arabidopsis voltage-dependent anion channel 1 (AtVDAC1) is required for female development and maintenance of mitochondrial functions related to energy-transaction.
abstract: The voltage-dependent anion channels (VDACs), prominently localized in the outer mitochondrial membrane, play important roles in the metabolite exchange, energy metabolism and mitochondria-mediated apoptosis process in mammalian cells. However, relatively little is known about the functions of VDACs in plants. To further investigate the function of AtVDAC1 in Arabidopsis, we analyzed a T-DNA insertion line for the AtVDAC1 gene. The knock-out mutant atvdac1 showed reduced seed set due to a large number of undeveloped ovules in siliques. Genetic analyses indicated that the mutation of AtVDAC1 affected female fertility and belonged to a sporophytic mutation. Abnormal ovules in the process of female gametogenesis were observed using a confocal laser scanning microscope. Interestingly, both mitochondrial transmembrane potential (ΔΨ) and ATP synthesis rate were obviously reduced in the mitochondria isolated from atvdac1 plants.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Xiaodi
authors.initials: X
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Ziwei
authors.initials: Z
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Xueyong
authors.initials: X
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Guoqin
authors.initials: G
authors.lastname: Liu
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004734:Energy Metabolism / Q000235:genetics*; D056130:Gametogenesis, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D053078:Membrane Potential, Mitochondrial / Q000235:genetics; D008928:Mitochondria / Q000235:genetics / Q000502:physiology*; D056771:Ovule / Q000235:genetics / Q000254:growth & development*; D063245:Plant Development / Q000235:genetics*; D030821:Plants, Genetically Modified; D050995:Voltage-Dependent Anion Channel 1 / Q000235:genetics / Q000502:physiology*
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA; D000255:Adenosine Triphosphate; D050995:Voltage-Dependent Anion Channel 1