title: An ultrasensitive label-free electrochemical biosensor for microRNA-21 detection based on a 2'-O-methyl modified DNAzyme and duplex-specific nuclease assisted target recycling.
abstract: Based on a highly efficient 2'-O-methyl modified G-quadruplex-hemin DNAzyme and duplex-specific nuclease (DSN) assisted target recycling, a novel label-free electrochemical biosensor for microRNA-21 (miR-21) detection is developed here. By employing the strategy, this DNA biosensor can detect as low as 8 aM miR-21 and exhibits high discrimination ability even against a single-base mismatch.
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, P. R. China. cjh_huaxue@126.com.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wu
authors.forename: Zhijing
authors.initials: Z
authors.lastname: Liu
authors.forename: Shuxian
authors.initials: S
authors.lastname: Cai
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhao
authors.forename: Mei
authors.initials: M
authors.lastname: Chen
authors.forename: Yaokun
authors.initials: Y
authors.lastname: Xia
authors.forename: Chunyan
authors.initials: C
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Jinghua
authors.initials: J
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques; D001943:Breast Neoplasms / Q000378:metabolism; D021881:DNA, Catalytic / Q000737:chemistry; D055664:Electrochemical Techniques; D004720:Endonucleases / Q000737:chemistry; D005260:Female; D054856:G-Quadruplexes; D006427:Hemin / Q000737:chemistry; D006801:Humans; D035683:MicroRNAs / Q000032:analysis* / Q000378:metabolism; D044967:Serum / Q000737:chemistry
chemical_list: D021881:DNA, Catalytic; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D006427:Hemin; D004720:Endonucleases