title: [Research on glucose measuring technique by surface plasmon resonance based on thiol coupling].
abstract: In the glucose measuring technique by surface plasmon resonance, D-galactose/D-glucose binding protein (GGBP) that can specifically adsorb glucose was introduced, and high-precision specific detection of glucose concentration was realized. In the present paper, the GGBP protein was bound on the surface of SPR sensor through thiol coupling method. GGBP binding experiment was carried out on SPR sensor and then glucose concentration experiment was conducted with this sensor. The results indicated that the SPR sensor had good linearity, stability and repeatability in the range of 0.1-10 mg x dL(-1). SPR sensor bound with GGBP would have great potential and vast development prospects.
authors.forename: Da-Chao
authors.initials: DC
authors.lastname: Li
authors.forename: Di
authors.initials: D
authors.lastname: Yang
authors.forename: Jia
authors.initials: J
authors.lastname: Yang
authors.forename: Jing-Xin
authors.initials: JX
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Wu
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Yu
authors.forename: Ke-Xin
authors.initials: KX
authors.lastname: Xu
mesh_terms: D005690:Galactose; D005947:Glucose / Q000032:analysis*; D009004:Monosaccharide Transport Proteins; D011485:Protein Binding; D013438:Sulfhydryl Compounds; D020349:Surface Plasmon Resonance
chemical_list: D009004:Monosaccharide Transport Proteins; D013438:Sulfhydryl Compounds; D005947:Glucose; D005690:Galactose