title: In Vitro selection of Neisseria gonorrhoeae mutants with elevated MIC values and increased resistance to cephalosporins.
abstract: Strains of Neisseria gonorrhoeae with mosaic penA genes bearing novel point mutations in penA have been isolated from ceftriaxone treatment failures. Such isolates exhibit significantly higher MIC values to third-generation cephalosporins. Here we report the in vitro isolation of two mutants with elevated MICs to cephalosporins. The first possesses a point mutation in the transpeptidase region of the mosaic penA gene, and the second contains an insertion mutation in pilQ.
authors.affiliation: Division of STD Prevention, NCHHSTP, Centers for Disease Control and Prevention, Atlanta, Georgia, USA SBJ1@cdc.gov.
authors.forename: Steven R
authors.initials: SR
authors.lastname: Johnson
authors.affiliation: Center for Communicable Disease Dynamics, Department of Epidemiology, Harvard School of Public Health, Boston, Massachusetts, USA Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Yonatan
authors.initials: Y
authors.lastname: Grad
authors.affiliation: Division of Scientific Resources, NCEZID, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Satishkumar Ranganathan
authors.initials: SR
authors.lastname: Ganakammal
authors.affiliation: Division of STD Prevention, NCHHSTP, Centers for Disease Control and Prevention, Atlanta, Georgia, USA Division of Scientific Resources, NCEZID, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Burroughs
authors.affiliation: Division of Scientific Resources, NCEZID, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Mike
authors.initials: M
authors.lastname: Frace
authors.affiliation: Center for Communicable Disease Dynamics, Department of Epidemiology, Harvard School of Public Health, Boston, Massachusetts, USA Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA.
authors.forename: Marc
authors.initials: M
authors.lastname: Lipsitch
authors.affiliation: Division of Scientific Resources, NCEZID, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: Weil
authors.affiliation: Division of STD Prevention, NCHHSTP, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: David
authors.initials: D
authors.lastname: Trees
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002511:Cephalosporins / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial; D004917:Erythromycin / Q000494:pharmacology; D036781:Fimbriae Proteins / Q000235:genetics*; D006069:Gonorrhea / Q000188:drug therapy / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D046915:Penicillin-Binding Proteins / Q000235:genetics; D017211:Treatment Failure; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D046915:Penicillin-Binding Proteins; C084295:pilQ protein, bacteria; D036781:Fimbriae Proteins; D004917:Erythromycin; D001618:beta-Lactamases