title: [Peroxiredoxin I in sperm and reactive oxygen species in seminal plasma in patients with idiopathic asthenozoospermia].
abstract: OBJECTIVE
  To explore the relationship between peroxiredoxin I expression and seminal reactive oxygen species (ROS) in patients with idiopathic asthenozoospermia.
  
  
  METHODS
  Twenty-six infertile male patients were selected from the Reproductive Medical Center, Xiangya Hospital, from September to December in 2012. Fresh semen was collected from an experimental group (26 idiopathic asthenozoospermia patients) and a control group (15 men with fertility history and normal semen). Luminol chemiluminescence method was applied to detect the seminal ROS level. Western blot was used to detect the peroxiredoxin I expression.
  
  
  RESULTS
  1)The seminal ROS level in the experimental group was significantly increased compared with that in the control group (P<0.05), and the seminal ROS level was negatively correlated with mobility of the sperm (r=-0.777, P<0.01). 2) Compared with the control group, the peroxiredoxin I expression was significantly downregulated in the experimental group (P<0.05). The content of sperm peroxiredoxin I in the 2 groups was negatively correlated with the seminal ROS level (r=-0.474, P<0.01). 3) The content of peroxiredoxin I had a positive correlation with human sperm motility(r=0.779, P<0.01).
  
  
  CONCLUSIONS
  The decline of peroxiredoxin I expression may be one of the crucial factors that leads to idiopathic asthenozoospermia. High level of ROS may be one of the main reasons for sperm vitality decline in patients with idiopathic asthenozoospermia.
authors.affiliation: Reproductive Medical Center, Department of Obstetrics and Gynaecology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.forename: Nenghui
authors.initials: N
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Zeng
mesh_terms: D053627:Asthenozoospermia; D016022:Case-Control Studies; D006801:Humans; D008297:Male; D054464:Peroxiredoxins / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000737:chemistry*; D012661:Semen / Q000737:chemistry*; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; C515940:PRDX1 protein, human; D054464:Peroxiredoxins