title: Stimuli-responsive conformational conversion of peptide gatekeepers for controlled release of guests from mesoporous silica nanocontainers.
abstract: The use of peptides as gatekeepers for payloads of mesoporous silica nanoparticles would allow triggering the release of guests by various biological stimuli. We investigated the effect of peptide conformation on their gatekeeping capability by employing two model peptides with a turn or a random structure. The conformation-dependent gatekeeping properties provided an opportunity to utilize the conformational conversion of peptides as a valuable motif for stimuli-responsive gatekeepers. Based on that investigation, we demonstrated that Fmoc-CGGC-SS-Si, which exhibited a zero-release property without any stimuli due to a turn-like conformation induced by the intramolecular disulfide bond, can be triggered to release guests by converting its conformation to a random structure, induced by reduction of the disulfide bond upon addition of glutathione. We further demonstrated that the conformational conversion of Fmoc-CGGC by Zn(II) ion can also be utilized as a triggering motif.
authors.affiliation: Department of Polymer Science and Engineering and ‡Department of Chemistry, Inha University , Incheon 402-751, Korea.
authors.forename: Jeonghun
authors.initials: J
authors.lastname: Lee
authors.forename: Hyunmi
authors.initials: H
authors.lastname: Kim
authors.forename: Songyi
authors.initials: S
authors.lastname: Han
authors.forename: Eunjung
authors.initials: E
authors.lastname: Hong
authors.forename: Keun-Hyeung
authors.initials: KH
authors.lastname: Lee
authors.forename: Chulhee
authors.initials: C
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000737:chemistry*; D005449:Fluorenes / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010455:Peptides / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D001386:Azides; D003692:Delayed-Action Preparations; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D010455:Peptides; D012822:Silicon Dioxide