title: MicroRNA-135b promotes proliferation, invasion and migration of osteosarcoma cells by degrading myocardin.
abstract: MicroRNA-135b (miR‑135b) functions as an oncogene in malignant tumors suggesting that it might be a target for malignant tumor therapy, but its role in osteosarcoma has not been investigated to date. In this study, we found that myocardin expression is specifically attenuated in MG63 osteosarcoma cells. Silencing miR‑135b restores the expression of myocardin and miR‑135b inhibits the expression of myocardin by targeting its 3'UTR in MG63 osteosarcoma cells. In additional experiments, we found that miR‑135b was highly expressed in osteosarcoma tissues and it promoted MG63 cell proliferation, migration and invasion. Myocardin had the opposite effects of miR‑135b, which suppressed proliferation, migration and invasion in MG63 cells. Thus, it is probably that miR‑135b promotes proliferation, invasion and migration of osteosarcoma cells by degrading myocardin.
authors.affiliation: Department of Orthopedics, Shanxian County Dongda Hospital, Shanxian, Heze, Shandong 274300, P.R. China.
authors.forename: Wei-Guo
authors.initials: WG
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, P.R. China.
authors.forename: Ying-Lie
authors.initials: YL
authors.lastname: Shang
authors.affiliation: Department of Orthopedics, Shanxian County Dongda Hospital, Shanxian, Heze, Shandong 274300, P.R. China.
authors.forename: Xian-Rui
authors.initials: XR
authors.lastname: Cong
authors.affiliation: Department of Oncology, The People's Hospital of Dongying, East District, Dongying, Shandong 257091, P.R. China.
authors.forename: Xia
authors.initials: X
authors.lastname: Bian
authors.affiliation: Department of Orthopedics, The 2nd People's Hospital of Dongying, Dawang Town, Guangrao, Dongying, Shandong 257335, P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D009687:Nuclear Proteins / Q000378:metabolism*; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D059748:Proteolysis; D015534:Trans-Activators / Q000378:metabolism*; D000099251:Myocardin
chemical_list: D035683:MicroRNAs; D009687:Nuclear Proteins; D015534:Trans-Activators; D000099251:Myocardin; C544965:MIRN135 microRNA, human