title: Neuronal-like cell differentiation of non-adherent bone marrow cell-derived mesenchymal stem cells.
abstract: Non-adherent bone marrow cell-derived mesenchymal stem cells from C57BL/6J mice were separated and cultured using the "pour-off" method. Non-adherent bone marrow cell-derived mesenchymal stem cells developed colony-forming unit-fibroblasts, and could be expanded by supplementation with epidermal growth factor. Immunocytochemistry showed that the non-adherent bone marrow cell-derived mesenchymal stem cells exposed to basic fibroblast growth factor/epidermal growth factor/nerve growth factor expressed the neuron specific markers, neurofilament-200 and NeuN, in vitro. Non-adherent bone marrow cell-derived mesenchymal stem cells from β-galactosidase transgenic mice were also transplanted into focal ischemic brain (right corpus striatum) of C57BL/6J mice. At 8 weeks, cells positive for LacZ and β-galactosidase staining were observed in the ischemic tissues, and cells co-labeled with both β-galactosidase and NeuN were seen by double immunohistochemical staining. These findings suggest that the non-adherent bone marrow cell-derived mesenchymal stem cells could differentiate into neuronal-like cells in vitro and in vivo.
authors.affiliation: Department of Neurosurgery, Nanjing Children's Hospital of Nanjing Medical University, Nanjing 210008, Jiangsu Province, China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Newborn Medicine, Nanjing Children's Hospital of Nanjing Medical University, Nanjing 210008, Jiangsu Province, China.
authors.forename: Jinghan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Newborn Medicine, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai 200040, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Ben
keywords: beta-galactosidase transgenic mouse; bone marrow cells-derived mesenchymal stem cells; cell transplantation; cerebral ischemia; colony-forming unit-fibroblasts; differentiation; grants-supported paper; neural regeneration; neuronal-like cells; neuroregeneration; non-adherent bone marrow cell-derived mesenchymal stem cells; proliferation; stem cells