title: The impact of dialysis modality on novel markers of stress reaction, matrix remodeling and endothelial damage in children on chronic dialysis.
abstract: OBJECTIVE
  Dialysis triggers stress reaction, matrix remodeling and endothelial damage, but little is known about the changes it induces on selected heat shock proteins (Hsp90α), adhesion molecules (E-cadherin, sE-selectin), metalloproteinases (MMP-8) and their extracellular inducer (EMMPRIN). The aim of this study was to assess serum concentrations of the above-mentioned parameters in children on chronic dialysis.
  
  
  METHODS
  19 patients on hemodialysis (HD), 22 children on peritoneal dialysis (PD) and 30 age-matched controls were examined. Serum concentrations of parameters were assessed by ELISA.
  
  
  RESULTS
  Hsp90α, MMP-8, EMMPRIN and E-cadherin concentrations were significantly increased in children on dialysis vs. controls and higher levels were in HD than PD patients. There was no difference in the level of sE-selectin between HD and PD modalities. A single HD session diminished Hsp90α, MMP-8, EMMPRIN and E-cadherin values, but had no impact on sE-selectin levels.
  
  
  CONCLUSIONS
  Hemodialysis evokes stress reaction, matrix and endothelium destruction, to a greater extent than peritoneal dialysis. Single hemodialysis influences circulating cells rather than endothelial cells.
authors.affiliation: Department of Pediatric Nephrology, Wrocław Medical University, Wrocław, Poland.
authors.forename: Kinga
authors.initials: K
authors.lastname: Musiał
authors.forename: Danuta
authors.initials: D
authors.lastname: Zwolińska
mesh_terms: D051926:Basigin / Q000097:blood / Q000235:genetics; D015415:Biomarkers / Q000097:blood; D015820:Cadherins / Q000097:blood / Q000235:genetics; D016022:Case-Control Studies; D002648:Child; D003430:Cross-Sectional Studies; D019040:E-Selectin / Q000097:blood / Q000235:genetics; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D005109:Extracellular Matrix / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D018841:HSP90 Heat-Shock Proteins / Q000097:blood / Q000235:genetics; D006801:Humans; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000097:blood / Q000235:genetics; D018384:Oxidative Stress; D006435:Renal Dialysis / Q000379:methods*; D051436:Renal Insufficiency, Chronic / Q000097:blood* / Q000473:pathology / Q000628:therapy*; D012189:Retrospective Studies
chemical_list: C497886:BSG protein, human; D015415:Biomarkers; D015820:Cadherins; D019040:E-Selectin; D018841:HSP90 Heat-Shock Proteins; C533585:HSP90AA2P protein, human; C546936:SELE protein, human; D051926:Basigin; D020784:Matrix Metalloproteinase 8