title: Down-regulation of Slit-Robo pathway mediating neuronal cytoskeletal remodeling processes facilitates the antidepressive-like activity of Gastrodia elata Blume.
abstract: Nowadays, depression is a serious psychological disorder that causes extreme economic loss and social problems. Previously, we discovered that the water extract of Gastrodia elata Blume (WGE) improved depressive-like behavior by influencing neurotransmitters in rats subjected to the forced swimming test. To elucidate possible mechanisms, in the present study, we performed a proteomics and bioinformatics analysis to identify the related pathways. Western blot-validated results indicated that the core protein network modulated by WGE administration was closely associated with down-regulation of the Slit-Robo pathway, which modulates neuronal cytoskeletal remodeling processes. Although Slit-Robo signaling has been well investigated in neuronal development, its relationship with depression is not fully understood. We provide a potential hint on the mechanism responsible for the antidepressive-like activity of WGE. In conclusion, we suggest that the Slit-Robo pathway and neuronal cytoskeleton remodeling are possibly one of the pathways associated with the antidepressive-like effects of WGE.
authors.affiliation: Institute of Food Science and Technology, National Taiwan University , Taipei, Taiwan.
authors.forename: Shih-Hang
authors.initials: SH
authors.lastname: Lin
authors.forename: Wei-Cheng
authors.initials: WC
authors.lastname: Chen
authors.forename: Kuan-Hung
authors.initials: KH
authors.lastname: Lu
authors.forename: Pei-Ju
authors.initials: PJ
authors.lastname: Chen
authors.forename: Shu-Chen
authors.initials: SC
authors.lastname: Hsieh
authors.forename: Tzu-Ming
authors.initials: TM
authors.lastname: Pan
authors.forename: Shui-Tein
authors.initials: ST
authors.lastname: Chen
authors.forename: Lee-Yan
authors.initials: LY
authors.lastname: Sheen
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000008:administration & dosage*; D003599:Cytoskeleton / Q000378:metabolism*; D003863:Depression / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects*; D031668:Gastrodia / Q000737:chemistry*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D000097905:Roundabout Proteins; D000098552:Slit Homolog 2 Protein
chemical_list: D000928:Antidepressive Agents; D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D010936:Plant Extracts; D011971:Receptors, Immunologic; D000097905:Roundabout Proteins; D000098552:Slit Homolog 2 Protein; C516057:Robo2 protein, rat; C492288:Slit1 protein, rat
keywords: Gastrodia elata Blume; Slit−Robo pathway; antidepressive-like; depression; proteomics