title: Plasma miR-454-3p as a potential prognostic indicator in human glioma.
abstract: microRNAs (miRNAs) are a class of small non-coding RNAs, approximately 21-25 nucleotides in length. Recently, some researchers have demonstrated that plasma miRNAs are sensitive and specific biomarkers of various cancers. Using quantitative real-time PCR, the expression levels of miR-454-3p were compared between pre-operative plasmas from 70 glioma patients and 70 healthy controls, and between these pre-operative and post-operative plasmas. Kaplan-Meier analysis was used to evaluate the association of miR-454-3p with prognosis of glioma patients. The expression levels of miR-454-3p in plasma in glioma patients were significantly higher than that from healthy controls. The expression levels of miR-454-3p were higher in high grade gliomas than in low grade gliomas. The AUC of the expression of miR-454-3p in plasma for glioma diagnosis was 0.9063. The expression levels of miR-454-3p in the post-operative plasmas were significantly downregulated when compared to the pre-operative plasmas. Moreover, the prognosis of glioma with high miR-454-3p expression was significantly worse compared with that of glioma with low miR-454-3p expression. Our results suggested that plasma miR-454-3p could be a novel potential diagnostic biomarker for glioma.
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, #1055 Sanxiang Road, Suzhou, 215004, Jiangsu, China.
authors.forename: Naiyuan
authors.initials: N
authors.lastname: Shao
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Lian
authors.initials: L
authors.lastname: Xue
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Lan
mesh_terms: D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood; D001932:Brain Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000473:pathology / Q000601:surgery; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000097:blood* / Q000175:diagnosis / Q000473:pathology / Q000601:surgery; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D060787:Neoplasm Grading; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C583712:MIRN454 microRNA, human; D035683:MicroRNAs