title: Scalable high-density peptide arrays for comprehensive health monitoring.
abstract: There is an increasing awareness that health care must move from post-symptomatic treatment to presymptomatic intervention. An ideal system would allow regular inexpensive monitoring of health status using circulating antibodies to report on health fluctuations. Recently, we demonstrated that peptide microarrays can do this through antibody signatures (immunosignatures). Unfortunately, printed microarrays are not scalable. Here we demonstrate a platform based on fabricating microarrays (~10 M peptides per slide, 330,000 peptides per assay) on silicon wafers using equipment common to semiconductor manufacturing. The potential of these microarrays for comprehensive health monitoring is verified through the simultaneous detection and classification of six different infectious diseases and six different cancers. Besides diagnostics, these high-density peptide chips have numerous other applications both in health care and elsewhere.
authors.affiliation: Center for Innovations in Medicine, Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA.
authors.forename: Joseph Barten
authors.initials: JB
authors.lastname: Legutki
authors.affiliation: Center for Innovations in Medicine, Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA.
authors.forename: Zhan-Gong
authors.initials: ZG
authors.lastname: Zhao
authors.affiliation: NextVal, 4186 Sorrento Valley Boulevard, Suite G, San Diego, California 92121, USA.
authors.forename: Matt
authors.initials: M
authors.lastname: Greving
authors.affiliation: Center for Innovations in Medicine, Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA.
authors.forename: Neal
authors.initials: N
authors.lastname: Woodbury
authors.affiliation: Center for Innovations in Medicine, Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA.
authors.forename: Stephen Albert
authors.initials: SA
authors.lastname: Johnston
authors.affiliation: Center for Innovations in Medicine, Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA.
authors.forename: Phillip
authors.initials: P
authors.lastname: Stafford
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000097:blood*; D000918:Antibody Specificity; D003141:Communicable Diseases / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D006304:Health Status; D006801:Humans; D056656:Lab-On-A-Chip Devices; D055616:Microtechnology / Q000379:methods; D008969:Molecular Sequence Data; D008991:Monitoring, Physiologic / Q000295:instrumentation / Q000379:methods; D009369:Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D011786:Quality Control; D012825:Silicon / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D010455:Peptides; D012825:Silicon