title: A click chemistry strategy for visualization of plant cell wall lignification.
abstract: Bioorthogonal click chemistry was commissioned to visualize the plant cell wall lignification process in vivo. This approach uses chemical reporter-tagged monolignol mimics that can be metabolically incorporated into lignins and subsequently derivatized via copper-assisted or copper-free click reactions.
authors.affiliation: Department of Biochemistry and the US Department of Energy's Great Lakes Bioenergy Research Center (GLBRC), the Wisconsin Energy Institute, University of Wisconsin, Madison, WI 53726, USA. jralph@wisc.edu yukitobi@kais.kyoto-u.ac.jp.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Tobimatsu
authors.forename: Dorien
authors.initials: D
authors.lastname: Van de Wouwer
authors.forename: Eric
authors.initials: E
authors.lastname: Allen
authors.forename: Robert
authors.initials: R
authors.lastname: Kumpf
authors.forename: Bartel
authors.initials: B
authors.lastname: Vanholme
authors.forename: Wout
authors.initials: W
authors.lastname: Boerjan
authors.forename: John
authors.initials: J
authors.lastname: Ralph
mesh_terms: D000480:Alkynes / Q000737:chemistry; D017360:Arabidopsis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D001386:Azides / Q000737:chemistry; D002473:Cell Wall / Q000378:metabolism*; D057930:Click Chemistry; D003300:Copper; D008031:Lignin / Q000737:chemistry / Q000378:metabolism*; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D059828:Plant Cells / Q000378:metabolism*
chemical_list: D000480:Alkynes; D001386:Azides; D010636:Phenols; D003300:Copper; D008031:Lignin; C010559:coniferyl alcohol