title: 8-hydroxy-2-(di-n-propylamino)tetralin intervenes with neural cell apoptosis following diffuse axonal injury.
abstract: Previous studies have reported a neuroprotective effect of 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT) against traumatic brain injury. In accordance with the Marmarou method, rat models of diffuse axonal injury were established. 8-OH-DPAT was intraperitoneally injected into model rats. 8-OH-DPAT treated rats maintained at constant temperature served as normal temperature controls. TUNEL results revealed that neural cell swelling, brain tissue necrosis and cell apoptosis occurred around the injured tissue. Moreover, the number of Bax-, Bcl-2- and caspase-3-positive cells increased at 6 hours after diffuse axonal injury, and peaked at 24 hours. However, brain injury was attenuated, the number of apoptotic cells reduced, Bax and caspase-3 expression decreased, and Bcl-2 expression increased at 6, 12, 24, 72 and 168 hours after diffuse axonal injury in normal temperature control and in 8-OH-DPAT-intervention rats. The difference was most significant at 24 hours. All indices in 8-OH-DPAT-intervention rats were better than those in the constant temperature group. These results suggest that 8-OH-DPAT inhibits Bax and caspase-3 expression, increases Bcl-2 expression, and reduces neural cell apoptosis, resulting in neuroprotection against diffuse axonal injury. This effect is associated with a decrease in brain temperature.
authors.affiliation: Department of Neurosurgery, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China ; Postgraduate School, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China.
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Mao
authors.affiliation: Department of Neurosurgery, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China.
authors.forename: Zhenquan
authors.initials: Z
authors.lastname: Song
authors.affiliation: Jilin Brain Hospital, Siping 136000, Jilin Province, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China ; Postgraduate School, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lv
authors.affiliation: Department of Neurosurgery, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China ; Postgraduate School, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China ; Postgraduate School, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China.
authors.forename: Xinli
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Neurosurgery, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China.
authors.forename: Youli
authors.initials: Y
authors.lastname: Chen
keywords: 8-hydroxy-2-(di-n-propylamino)tetralin; Bax; Bcl-2; brain injury; caspase-3; cell apoptosis; diffuse axonal injury; grant-supported paper; mild hypothermia; neural regeneration; neuroprotection; neuroregeneration; photographs-containing paper