title: Anti-apoptotic effect of taxodione on serum/glucose deprivation-induced PC12 cells death.
abstract: Taxodione, a diterpenoid from the roots of Salvia chorassanica Bunge, possesses cytotoxic, apoptotic, and antimicrobial activity. This study was designed to investigate the protective effects of taxodione on serum/glucose deprivation-induced ischemic injury in PC12 cells and related mechanisms. In an in vitro model of ischemia, PC12 cells were exposed to serum and glucose deprivation for 6 and 18 h. The protective effects of the methanol extract of S. chorassanica and taxodione were assessed using alamarBlue(®) assay. Intracellular ROS production was measured by fluorimetry using 2',7'-dichlorofluorescin diacetate (DCFH-DA). The levels of PARP, Bcl-2, and Bax proteins were detected after western blot analysis. It was shown that taxodione (0.2-1.5 μM) significantly increased cell viability in a dose-dependent manner after ischemic insult. Taxodione has antioxidant activity and protects PC12 cells against oxidative stress-induced apoptotic cell death. Meanwhile, pretreatment with taxodione significantly induced an increase in Bcl-2 and a decrease in Bax protein level. The results of this study confirmed the protective effect of taxodione in serum/glucose deprivation-induced ischemic injury and the putative role of apoptosis as a underling mechanisms. Thus, it would be fair to consider taxodione as a promising ingredient of S. chorassanica for the expansion on novel class of anti-ischemic agents.
authors.affiliation: Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, P.O. Box 9188617871, Mashhad, Iran.
authors.forename: Negar
authors.initials: N
authors.lastname: Shafaei-Bajestani
authors.forename: Seyed Ahmad
authors.initials: SA
authors.lastname: Emami
authors.forename: Javad
authors.initials: J
authors.lastname: Asili
authors.forename: Zahra
authors.initials: Z
authors.lastname: Tayarani-Najaran
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004224:Diterpenes / Q000494:pharmacology*; D005470:Fluorometry; D005947:Glucose / Q000172:deficiency*; D018696:Neuroprotective Agents / Q000494:pharmacology; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D027544:Salvia / Q000737:chemistry; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D016895:Culture Media, Serum-Free; D004224:Diterpenes; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D005947:Glucose; C009387:taxodione; D000111:Acetylcysteine