title: Lateral flow test using Echinococcus granulosus native antigen B and comparison of IgG and IgG4 dipsticks for detection of human cystic echinococcosis.
abstract: Cystic echinococcosis (CE) caused by infection with Echinococcus granulosus is of major concern for humans in many parts of the world. Antigen B was prepared from E. granulosus hydatid fluid, and Western blots confirmed eight batches showing a band corresponding to the 8-/12-kDa subunit with positive serum and no low-molecular mass band (< 15 kDa) with negative serum. The batches were pooled and used to prepare lateral flow immunoglobulin G4 (IgG4) and IgG dipsticks. Diagnostic sensitivity was determined using serum samples from 21 hydatidosis patients, and diagnostic specificity was established using sera from 17 individuals infected with other parasites and 15 healthy people. IgG4 dipstick had a diagnostic sensitivity of 95% (20 of 21) and a specificity of 100% (32 of 32). The IgG dipstick had a sensitivity of 100% (21 of 21) and a specificity of 87.5% (28 of 32). Thus, both IgG and IgG4 dipsticks had high sensitivities, but IgG4 had greater specificity for the diagnosis of human CE.
authors.affiliation: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia; Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Akbar
authors.initials: A
authors.lastname: Khalilpour
authors.affiliation: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia; Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran rahmah8485@gmail.com sadjjadi316@gmail.com.
authors.forename: Seyed Mahmoud
authors.initials: SM
authors.lastname: Sadjjadi
authors.affiliation: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia; Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Zohreh Kazemi
authors.initials: ZK
authors.lastname: Moghadam
authors.affiliation: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia; Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Muhammad Hafiznur
authors.initials: MH
authors.lastname: Yunus
authors.affiliation: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia; Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Nor Dyana
authors.initials: ND
authors.lastname: Zakaria
authors.affiliation: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia; Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Sabariah
authors.initials: S
authors.lastname: Osman
authors.affiliation: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia; Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran rahmah8485@gmail.com sadjjadi316@gmail.com.
authors.forename: Rahmah
authors.initials: R
authors.lastname: Noordin
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology; D000947:Antigens, Helminth / Q000097:blood / Q000276:immunology; D004443:Echinococcosis / Q000175:diagnosis* / Q000276:immunology*; D048209:Echinococcus granulosus / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006046:Gold; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008074:Lipoproteins / Q000097:blood / Q000276:immunology*; D053768:Metal Nanoparticles / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D008074:Lipoproteins; C514022:antigen B, Echinococcus granulosus; D006046:Gold