title: Probing single-tumor cell interactions with different-age type I collagen networks by synchrotron-based Fourier transform infrared microspectroscopy.
abstract: We report here on a first study using synchrotron radiation-based Fourier transform infrared microspectroscopy and imaging to investigate HT1080 human fibrosarcoma cells grown onto different-aged type I collagen networks. Spectral images were analyzed with k-means and fuzzy C-means (FCM) clustering algorithms. K-means delineated tumor cells from their surrounding collagen networks and the latter as a function of age mainly due to specific changes in the sugar absorption region. The FCM analysis gave a better nuance of the spectral images. A progression of the biochemical information was observed upon going from the cellular compartments to the pericellular contact regions and to the intact collagens of the different age groups. Two spectral markers based on sugar and protein bands via the intensity ratio (I1032/I1655) and band area ratio (Asugar/Aamide II), showed an increase in advanced glycation endproducts (AGEs) with age. A clear-separation of the three age groups was obtained for spectra originating from the peripheral contact areas mainly due to changes in protein band intensities. The above-described markers decreased to constant levels for the three conditions indicating a masking of the biochemical information. These results hold promises to better understand the impact of age on tumor progression processes while highlighting new markers of the tumor cell invasion front.
authors.affiliation: Université de Reims Champagne-Ardenne, Equipe MéDIAN-Biophotonique et Technologies pour la Santé, UFR de Pharmacie, 51 rue Cognacq-Jay, 51096 Reims, FrancebCNRS UMR7369, Matrice Extracellulaire et Dynamique Cellulaire, 51096 Reims, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Guilbert
authors.affiliation: Université de Reims Champagne-Ardenne, Equipe MéDIAN-Biophotonique et Technologies pour la Santé, UFR de Pharmacie, 51 rue Cognacq-Jay, 51096 Reims, FrancebCNRS UMR7369, Matrice Extracellulaire et Dynamique Cellulaire, 51096 Reims, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Eklouh-Molinier
authors.affiliation: Diamond Light Source, Diamond House, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom.
authors.forename: Katia
authors.initials: K
authors.lastname: Wehbe
authors.affiliation: Keele University, Institute for Science and Technology in Medicine, Stoke-on-Trent, ST4 7QB, United KingdomeUniversity Hospital of North Staffordshire, Cancer Centre, Newcastle Road, Stoke-on-Trent ST4 6QG, United Kingdom.
authors.forename: Josep
authors.initials: J
authors.lastname: Sulé-Suso
authors.affiliation: Keele University, Institute for Science and Technology in Medicine, Stoke-on-Trent, ST4 7QB, United Kingdom.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Diamond Light Source, Diamond House, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom.
authors.forename: Gianfelice
authors.initials: G
authors.lastname: Cinque
authors.affiliation: Université de Reims Champagne-Ardenne, Equipe MéDIAN-Biophotonique et Technologies pour la Santé, UFR de Pharmacie, 51 rue Cognacq-Jay, 51096 Reims, FrancebCNRS UMR7369, Matrice Extracellulaire et Dynamique Cellulaire, 51096 Reims, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Jeannesson
authors.affiliation: Université de Reims Champagne-Ardenne, Equipe MéDIAN-Biophotonique et Technologies pour la Santé, UFR de Pharmacie, 51 rue Cognacq-Jay, 51096 Reims, FrancebCNRS UMR7369, Matrice Extracellulaire et Dynamique Cellulaire, 51096 Reims, France.
authors.forename: Ganesh D
authors.initials: GD
authors.lastname: Sockalingum
mesh_terms: D000465:Algorithms; D000818:Animals; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000378:metabolism; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D024042:Collagen Type I / Q000737:chemistry / Q000378:metabolism*; D009369:Neoplasms / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D059010:Single-Cell Analysis / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D017356:Synchrotrons
chemical_list: D014408:Biomarkers, Tumor; D024042:Collagen Type I