title: Paracrine effect of mesenchymal stem cells derived from human adipose tissue in bone regeneration.
abstract: Mesenchymal stem cell (MSC) transplantation has proved to be a promising strategy in cell therapy and regenerative medicine. Although their mechanism of action is not completely clear, it has been suggested that their therapeutic activity may be mediated by a paracrine effect. The main goal of this study was to evaluate by radiographic, morphometric and histological analysis the ability of mesenchymal stem cells derived from human adipose tissue (Ad-MSC) and their conditioned medium (CM), to repair surgical bone lesions using an in vivo model (rabbit mandibles). The results demonstrated that both, Ad-MSC and CM, induce bone regeneration in surgically created lesions in rabbit's jaws, suggesting that Ad-MSC improve the process of bone regeneration mainly by releasing paracrine factors. The evidence of the paracrine effect of MSC on bone regeneration has a major impact on regenerative medicine, and the use of their CM can address some issues and difficulties related to cell transplants. In particular, CM can be easily stored and transported, and is easier to handle by medical personnel during clinical procedures.
authors.affiliation: Faculty of Dentistry, Universidad Nacional de Colombia, Bogotá, Colombia.
authors.forename: Itali
authors.initials: I
authors.lastname: Linero
authors.affiliation: Faculty of Medicine, Universidad Nacional de Colombia, Bogotá, Colombia.
authors.forename: Orlando
authors.initials: O
authors.lastname: Chaparro
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects; D000328:Adult; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D005260:Female; D006801:Humans; D020100:Hydrogels; D007124:Immunoenzyme Techniques; D007568:Jaw / Q000166:cytology* / Q000187:drug effects; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D056948:Orthognathic Surgical Procedures; D019899:Paracrine Communication; D011817:Rabbits; D044968:Regenerative Medicine; D014945:Wound Healing; D055815:Young Adult
chemical_list: D017077:Culture Media, Conditioned; D020100:Hydrogels