title: Development of an artificial calcium-dependent transcription factor to detect sustained intracellular calcium elevation.
abstract: The development of a synthetic transcription factor that responds to intracellular calcium signals enables analyzing cellular events at the single-cell level or "rewiring" the intracellular information networks. In this study, we developed the calcium-dependent transcription factor (CaTF), which was cleaved by calpain and then translocated to the nuclei where it induced reporter expression. Our results demonstrated that CaTF-mediated reporter expression was stable and responded to the intracellular calcium level and calpain activity. In addition, CaTF detected the sustained calcium increase that was induced by physiological stimulation with epidermal growth factor (EGF). These results suggest that CaTF could be a useful tool to analyze intracellular calcium signals and be an interface between an endogenous signal network and synthetic gene network.
authors.affiliation: Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University , Miki-cho, Kagawa 761-0793, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Suzuki
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Murotomi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nakajima
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Kawai
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Ohta
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Warita
authors.forename: Takanori
authors.initials: T
authors.lastname: Miki
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Takeuchi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D006224:Cricetinae; D004815:Epidermal Growth Factor / Q000378:metabolism; D015870:Gene Expression; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D058615:Synthetic Biology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D014157:Transcription Factors; D004815:Epidermal Growth Factor; D002118:Calcium
keywords: biosensor; cell therapy; interface; intracellular calcium; mammalian synthetic biology; synthetic transcription factor