title: Synthesis of [³²P]NAADP for the radioreceptor binding assay.
abstract: Nicotinic acid adenine dinucleotide phosphate (NAADP) is a major messenger for Ca(2+) mobilization in cells. NAADP-binding proteins are highly selective and have a strong affinity for NAADP. This is the basis of the radioreceptor binding assay, which is used to measure NAADP levels in cells and tissues and to identify cellular stimuli that use NAADP as an intracellular messenger. In the radioreceptor binding assay, radiolabeled NAADP ([(32)P]NAADP) competes with endogenous NAADP present in samples for binding to their receptors. Here, we describe the synthesis of [(32)P]NAADP for use in the radioreceptor binding assay.
authors.affiliation: Department of Pharmacology, University of Oxford, Oxford OX1 3QT, United Kingdom.
authors.forename: Antony
authors.initials: A
authors.lastname: Galione
authors.affiliation: Department of Pharmacology, University of Oxford, Oxford OX1 3QT, United Kingdom.
authors.forename: Kai-Ting
authors.initials: KT
authors.lastname: Chuang
authors.affiliation: Department of Pharmacology, University of Oxford, Oxford OX1 3QT, United Kingdom.
authors.forename: Tim M
authors.initials: TM
authors.lastname: Funnell
authors.affiliation: Department of Pharmacology, University of Oxford, Oxford OX1 3QT, United Kingdom.
authors.forename: Lianne C
authors.initials: LC
authors.lastname: Davis
authors.affiliation: Department of Pharmacology, University of Oxford, Oxford OX1 3QT, United Kingdom.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Morgan
authors.affiliation: Department of Pharmacology, University of Oxford, Oxford OX1 3QT, United Kingdom.
authors.forename: Margarida
authors.initials: M
authors.lastname: Ruas
authors.affiliation: Department of Pharmacology, University of Oxford, Oxford OX1 3QT, United Kingdom.
authors.forename: John
authors.initials: J
authors.lastname: Parrington
authors.affiliation: Department of Pharmacology, University of Oxford, Oxford OX1 3QT, United Kingdom.
authors.forename: Grant C
authors.initials: GC
authors.lastname: Churchill
mesh_terms: D001665:Binding Sites / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002247:Carbon Isotopes / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D009249:NADP / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000493:pharmacokinetics; D011869:Radioligand Assay
chemical_list: D002247:Carbon Isotopes; D009249:NADP; C024376:NAADP; D002118:Calcium