title: Hyperspectral imaging and characterization of live cells by broadband coherent anti-Stokes Raman scattering (CARS) microscopy with singular value decomposition (SVD) analysis.
abstract: Coherent anti-Stokes Raman scattering (CARS) microscopy can be used as a powerful imaging technique to identify chemical compositions of complex samples in biology, biophysics, medicine, and materials science. In this work we developed a CARS microscopic system capable of hyperspectral imaging. By employing an ultrafast laser source, a photonic crystal fiber, and a scanning laser microscope together with spectral detection by a highly sensitive back-illuminated cooled charge-coupled device (CCD) camera, we were able to rapidly acquire and process hyperspectral images of live cells with chemical selectivity. We discuss various aspects of hyperspectral CARS image analysis and demonstrate the use of singular value decomposition methods to characterize the cellular lipid content.
authors.affiliation: Department of Chemistry, University of Michigan, 930 N. University, Ann Arbor, MI 48109 USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Khmaladze
authors.forename: Joshua
authors.initials: J
authors.lastname: Jasensky
authors.forename: Erika
authors.initials: E
authors.lastname: Price
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.forename: Andrew
authors.initials: A
authors.lastname: Boughton
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Han
authors.forename: Emily
authors.initials: E
authors.lastname: Seeley
authors.forename: Xinran
authors.initials: X
authors.lastname: Liu
authors.forename: Mark M
authors.initials: MM
authors.lastname: Banaszak Holl
authors.forename: Zhan
authors.initials: Z
authors.lastname: Chen
mesh_terms: D017667:Adipocytes / Q000737:chemistry* / Q000166:cytology; D000818:Animals; D002460:Cell Line; D008055:Lipids / Q000032:analysis / Q000737:chemistry; D051379:Mice; D008853:Microscopy / Q000379:methods*; D012815:Signal Processing, Computer-Assisted; D059010:Single-Cell Analysis / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D008055:Lipids