title: Variable transcription of pro- and anti-inflammatory cytokines in phocine lymphocytes following canine distemper virus infection.
abstract: Canine distemper virus (CDV) is a highly contagious viral pathogen. Domesticated dogs are the main reservoir of CDV. Although phocine distemper virus was responsible for the recent epidemics in seals in the North and Baltic Seas, most devastating epidemics in seals were also caused by CDV. To further study the pathogenesis of CDV infection in seals, it was the aim of the present study to investigate the mechanisms of CDV induced immunosuppression in seals by analyzing the gene transcription of different pro- and anti-inflammatory cytokines in Concanavalin A (Con A) stimulated and non-stimulated phocine lymphocytes in vitro following infection with the CDV Onderstepoort (CDV-OND) strain. Phocine lymphocytes were isolated via density gradient centrifugation. The addition of 1 μg/ml Con A and virus was either performed simultaneously or lymphocytes were stimulated for 48 h with Con A prior to virus infection. Gene transcription of interleukin (IL)-6, IL-12 and tumor necrosis factor alpha (TNFα) as pro-inflammatory cytokines and IL-4, IL-10 and transforming growth factor beta (TGFβ) as anti-inflammatory cytokines were determined by using RT-qPCR. CDV-OND infection caused an initial increase of pro-inflammatory phocine cytokines mRNA 24h after infection, followed by a decrease in gene transcription after 48 h. A strong increase in the transcription of IL-4 and TGFβ was detected after 48 h when virus and mitogen were added simultaneously. An increased IL-10 production occurred only when stimulation and infection were performed simultaneously. Furthermore, an inhibition of IL-12 on IL-4 was noticed in phocine lymphocytes which were stimulated for 48 h prior to infection. In summary, the duration of the stimulation or the lymphocytes seem to have an important influence on the cytokine transcription and indicates that the outcome of CDV infection is dependent on various factors that might sensitize lymphocytes or make them more susceptible or reactive to CDV infection.
authors.affiliation: Institute of Terrestrial and Aquatic Wildlife Research (ITAW), University of Veterinary Medicine, Hannover, Foundation, Werftstrasse 6, 25761 Büsum, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Seibel
authors.affiliation: Institute of Terrestrial and Aquatic Wildlife Research (ITAW), University of Veterinary Medicine, Hannover, Foundation, Werftstrasse 6, 25761 Büsum, Germany. Electronic address: ursula.siebert@tiho-hannover.de.
authors.forename: U
authors.initials: U
authors.lastname: Siebert
authors.affiliation: Seal Centre Friedrichskoog e.V., An der Seeschleuse 4, 25718 Friedrichskoog, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Rosenberger
authors.affiliation: Department of Pathology, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559 Hannover, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Baumgärtner
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004216:Distemper / Q000276:immunology* / Q000378:metabolism; D004217:Distemper Virus, Canine; D005786:Gene Expression Regulation / Q000276:immunology*; D008214:Lymphocytes / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D046048:Seals, Earless
chemical_list: D016207:Cytokines
keywords: Cytokines; Harbor seals; Lymphocytes; Mitogen; Morbillivirus