title: Danshensu-mediated protective effect against hepatic fibrosis induced by carbon tetrachloride in rats.
abstract: The culprit of hepatic fibrosis (HF) is linked to suprathreshold deposition of collagen. Thus, collagen reduction by improved metabolism contributes to HF management. In this study, we aimed to investigate the hepatoprotective effects of Danshensu (DSS) against carbon tetrachloride (CCl4)-induced HF rats. The results showed that DSS-administrated rats resulted in decreasing in hepatosomatic indexes, and lowering serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Meanwhile, the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) were increased, while the content of malonaldehyde (MDA) was lessened in liver tissue of DSS administration group. In addition, the pro-fibrotic markers of hydroxyproline (Hyp), type III procollagen (PCIII) and hyaluronic acid (HA) contents were decreased. Histopathological examination confirmed that the hepatotoxicity in CCl4-injured rats was alleviated following the DSS administration. Furthermore, intrahepatic protein expressions of alpha-smooth muscle actin (α-SMA), phosphorylated JAK2 (p-JAK2) and phosphorylated STAT3 (p-STAT3) were effectively down-regulated, respectively. Overall, this work demonstrates that DSS played the protective effect against CCl4-induced cytotoxicity in liver tissue, which the probable mechanism is associated with attenuation of lipid peroxidation, collagen accumulation and enhancement of anti-oxidative defense capability, as well as regulation of intrahepatic JAK/STAT pathway for maintaining collagenic homoeostasis.
authors.affiliation: Department of Gastroenterology, Provincial Hospital, Shandong University, No. 324, Jingwuweiqi Road, Jinan, Shandong 250021, PR China; Department of Nosocomial Infection Management, The Central Hospital of Taian, Shandong 271000, PR China.
authors.forename: W
authors.initials: W
authors.lastname: Qu
authors.affiliation: Department of Gastroenterology, The Second Affiliated Hospital of Kunming Medical University, Yunnan 650101, PR China.
authors.forename: H
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Gynaecology and Obstetrics, Affiliated Hospital of Shandong Academy of Medical Sciences, Shandong 250031, PR China; Shandong Academy of Medical Sciences, Shandong 250062, PR China.
authors.forename: K
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Provincial Hospital, Shandong University, No. 324, Jingwuweiqi Road, Jinan, Shandong 250021, PR China. Electronic address: qcy_hospital@126.com.
authors.forename: C
authors.initials: C
authors.lastname: Qin
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002251:Carbon Tetrachloride; D019610:Cytoprotection / Q000187:drug effects*; D007773:Lactates / Q000494:pharmacology* / Q000627:therapeutic use; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D008111:Liver Function Tests; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007773:Lactates; C035055:3,4-dihydroxyphenyllactic acid; D002251:Carbon Tetrachloride
keywords: Carbon tetrachloride; Danshensu; Fibrose hépatique; Hepatic fibrosis; Hepatoprotection; Hépatoprotection; Metabolism; Métabolisme