title: PLGA nanofiber membranes loaded with epigallocatechin-3-O-gallate are beneficial to prevention of postsurgical adhesions.
abstract: This study concentrates on the development of biodegradable nanofiber membranes with controlled drug release to ensure reduced tissue adhesion and accelerated healing. Nanofibers of poly(lactic-co-glycolic acid) (PLGA) loaded with epigallocatechin-3-O-gallate (EGCG), the most bioactive polyphenolic compound in green tea, were electrospun. The physicochemical and biomechanical properties of EGCG-releasing PLGA (E-PLGA) nanofiber membranes were characterized by atomic force microscopy, EGCG release and degradation profiles, and tensile testing. In vitro antioxidant activity and hemocompatibility were evaluated by measuring scavenged reactive oxygen species levels and activated partial thromboplastin time, respectively. In vivo antiadhesion efficacy was examined on the rat peritonea with a surgical incision. The average fiber diameter of E-PLGA membranes was approximately 300-500 nm, which was almost similar to that of pure PLGA equivalents. E-PLGA membranes showed sustained EGCG release mediated by controlled diffusion and PLGA degradation over 28 days. EGCG did not adversely affect the tensile strength of PLGA membranes, whereas it significantly decreased the elastic modulus and increased the strain at break. E-PLGA membranes were significantly effective in both scavenging reactive oxygen species and extending activated partial thromboplastin time. Macroscopic observation after 1 week of surgical treatment revealed that the antiadhesion efficacy of E-PLGA nanofiber membranes was significantly superior to those of untreated controls and pure PLGA equivalents, which was comparable to that of a commercial tissue-adhesion barrier. In conclusion, the E-PLGA hybrid nanofiber can be exploited to craft strategies for the prevention of postsurgical adhesions.
authors.affiliation: Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, Republic of Korea.
authors.forename: Yong Cheol
authors.initials: YC
authors.lastname: Shin
authors.affiliation: Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, Republic of Korea.
authors.forename: Won Jun
authors.initials: WJ
authors.lastname: Yang
authors.affiliation: Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, Republic of Korea.
authors.forename: Jong Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Nanomaterials Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan, Republic of Korea.
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Oh
authors.affiliation: Department of Design, College of Arts, Pusan National University, Busan, Republic of Korea.
authors.forename: Tai Wan
authors.initials: TW
authors.lastname: Kim
authors.affiliation: Department of Medical Engineering, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors.forename: Jong-Chul
authors.initials: JC
authors.lastname: Park
authors.affiliation: Center for Fiber and Textile Science, Kyoto Institute of Technology, Kyoto, Japan.
authors.forename: Suong-Hyu
authors.initials: SH
authors.lastname: Hyon
authors.affiliation: Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, Republic of Korea.
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Han
mesh_terms: D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D019344:Lactic Acid / Q000737:chemistry* / Q000494:pharmacology; D008297:Male; D008567:Membranes, Artificial; D057139:Nanofibers / Q000737:chemistry*; D010314:Partial Thromboplastin Time; D010537:Peritoneum / Q000187:drug effects / Q000473:pathology; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D020011:Protective Agents / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000267:Tissue Adhesions / Q000473:pathology / Q000517:prevention & control*
chemical_list: D008567:Membranes, Artificial; D020011:Protective Agents; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D002392:Catechin; C045651:epigallocatechin gallate
keywords: antiadhesion; epigallocatechin-3-O-gallate; nanofiber membrane; poly(lactic-co-glycolic acid); tissue-adhesion barrier