title: Nucleic acid-induced tetraphenylethene probe noncovalent self-assembly and the superquenching of aggregation-induced emission.
abstract: Superquenching of aggregation-induced emission (AIE) has been utilized in biosensing for the first time. A positively charged tetraphenylethene derivative (compound 1) showed no emission in an aqueous buffer solution. A single-stranded DNA (a polyanion) induced aggregation of compound 1, and strong compound 1 aggregate emission was observed. When the DNA was labeled with a quencher molecule, compound 1 aggregate emission was efficiently quenched. On the basis of this observation, a new, simple, sensitive and selective DNA methyltransferase (MTase) assay has been developed. A quencher-labeled double-stranded DNA could induce aggregation of compound 1, and superquenching of compound 1 AIE was observed. In the presence of MTase and an endonuclease, the DNA could be specifically methylated and cleaved into single-stranded DNA fragments. The quencher molecule was released, and a turn-on emission signal was detected.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, P. R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Wenying
authors.initials: W
authors.lastname: Li
authors.forename: Huipeng
authors.initials: H
authors.lastname: Zhou
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Li
authors.forename: Cong
authors.initials: C
authors.lastname: Yu
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000737:chemistry; D019175:DNA Methylation; D004277:DNA, Single-Stranded / Q000032:analysis*; D004720:Endonucleases / Q000737:chemistry; D005030:Ethylenes / Q000737:chemistry*; D005427:Flocculation; D005456:Fluorescent Dyes / Q000737:chemistry; D015335:Molecular Probes / Q000737:chemistry*; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D055672:Static Electricity
chemical_list: D004247:DNA; D004248:DNA (Cytosine-5-)-Methyltransferases; D004277:DNA, Single-Stranded; D004720:Endonucleases; D005030:Ethylenes; D005456:Fluorescent Dyes; D015335:Molecular Probes; D000071228:Polyelectrolytes; D011108:Polymers