title: Electrically controlled delivery of cargo into single human neural stem cell.
abstract: Nanoprobe-based techniques have emerged as an efficient tool for the manipulation and analysis of single cells. Here, we report a powerful whole-electrical single-cell manipulation tool that enables rapid and controllable delivery of cargo into single neural stem cells with precision monitoring of the cell penetration process using a conductive nanoprobe. The highly electrically sensitive nanoprobes that were fabricated and the indium tin oxide electrode-integrated cell chip were found to be very effective for monitoring the cell penetration process via current changes that appear as spike-like negative currents. Moreover, the assembly of cargoes onto the nanoprobes was controllable and could reach its maximum load in a very short period of time (<10 min) based on the same electrical system that was used for monitoring cell penetration and without the need for any complex chemical linkers or mediators. Even more remarkably, the cargo assembled on the surface of the nanoprobe was successfully released in a very short period of time (<10 s), regardless of the surrounding intracellular or extracellular environments. The monitoring of cell penetration, assembly of quantum dots (QDs), and release of QDs into the intracellular environment were all accomplished using our whole-electrical system that combined a conductive nanoprobe with cell chip technology. This is a novel technology, which can eliminate complex and time-consuming steps owing to chemical modifications, as well as reduce the time needed for the delivery of cargo into the cell cytosol/nucleus during cell penetration, which is very important for reducing cell damage.
authors.affiliation: Department of Chemical & Biomolecular Engineering, Sogang University , 35 Baekbeom-ro, Mapo-gu, Seoul 121-742, Korea.
authors.forename: Tae-Hyung
authors.initials: TH
authors.lastname: Kim
authors.forename: Hyeon-Yeol
authors.initials: HY
authors.lastname: Cho
authors.forename: Ki-Bum
authors.initials: KB
authors.lastname: Lee
authors.forename: Seung U
authors.initials: SU
authors.lastname: Kim
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Choi
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004560:Electricity; D006801:Humans; D058608:Nanopores; D058953:Neural Stem Cells / Q000737:chemistry* / Q000166:cytology; D045663:Quantum Dots; D059010:Single-Cell Analysis; D017971:Tin Compounds / Q000737:chemistry
chemical_list: D017971:Tin Compounds; C109984:indium tin oxide
keywords: electrical assembly and release; human neural stem cell; nanoprobe; quantum dots; single-cell manipulation