title: Status epilepticus increases mature granule cells in the molecular layer of the dentate gyrus in rats.
abstract: Enhanced neurogenesis in the dentate gyrus of the hippocampus following seizure activity, especially status epilepticus, is associated with ectopic residence and aberrant integration of newborn granule cells. Hilar ectopic granule cells may be detrimental to the stability of dentate circuitry by means of their electrophysiological properties and synaptic connectivity. We hypothesized that status epilepticus also increases ectopic granule cells in the molecular layer. Status epilepticus was induced in male Sprague-Dawley rats by intraperitoneal injection of pilocarpine. Immunostaining showed that many doublecortin-positive cells were present in the molecular layer and the hilus 7 days after the induction of status epilepticus. At least 10 weeks after status epilepticus, the estimated number of cells positive for both prospero homeobox protein 1 and neuron-specific nuclear protein in the hilus was significantly increased. A similar trend was also found in the molecular layer. These findings indicate that status epilepticus can increase the numbers of mature and ectopic newborn granule cells in the molecular layer.
authors.affiliation: Institute of Physiology, Shandong University School of Medicine, Jinan 250012, Shandong Province, China.
authors.forename: Zhaoliang
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Institute of Physiology, Shandong University School of Medicine, Jinan 250012, Shandong Province, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.affiliation: Institute of Physiology, Shandong University School of Medicine, Jinan 250012, Shandong Province, China.
authors.forename: Fajun
authors.initials: F
authors.lastname: Wang
authors.affiliation: Institute of Physiology, Shandong University School of Medicine, Jinan 250012, Shandong Province, China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Physiology, Shandong University School of Medicine, Jinan 250012, Shandong Province, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Song
authors.affiliation: Institute of Physiology, Shandong University School of Medicine, Jinan 250012, Shandong Province, China.
authors.forename: Kejing
authors.initials: K
authors.lastname: Liu
authors.affiliation: Institute of Physiology, Shandong University School of Medicine, Jinan 250012, Shandong Province, China.
authors.forename: Ren-Zhi
authors.initials: RZ
authors.lastname: Zhan
keywords: basic research; dentate gyrus; doublecortin; grants-supported paper; granule cells; hippocampus; molecular layer; neural regeneration; neuron-specific nuclear protein; neuroregeneration; photographs-containing paper; prospero homeobox protein 1; status epilepticus