title: Evaluation of Hirsutella sinensis mycelium on food safety and anti-hepatoma activity in an animal model.
abstract: There is evidence that Hirsutella sinensis may have antitumor activity. The aim of the present study was to determine the anti-hepatoma effects and food safety assessment of Hirsutella sinensis mycelium in vivo and in vitro. Effects on mutagenicity were determined using a bacterial reverse mutation assay employing the Salmonella typhimurium strains TA98, TA100, TA102, TA1535 and TA1537. There were no dose-dependent increases or decreases in the number of colonies both with and without metabolic S9 activation in Ames tests. Mice were inoculated with SK-Hep 1 cells and those developing tumors were treated with three different concentrations of Hirsutella sinensis mycelium. After six weeks, blood samples were collected and liver pathology was determined. Aspartate aminotransferase levels were significantly different only in the low-dose treatment group (106±27 IU/l, p=0.048), compared to the control group (162±80 IU/l). The tumor weight was significantly different only in the low-dose treatment group. We found that necrosis, hemorrhage and calcifications were presented in both control and experimental groups. Inhibition of tumor growth was observed only at the lowest dose.
authors.affiliation: Department of Pathology, National Defense Medical Center, Division of Clinical Pathology, Tri-Service General Hospital, Taipei, Taiwan, R.O.C.
authors.forename: Hung-Sheng
authors.initials: HS
authors.lastname: Shang
authors.affiliation: Department of Anatomical Pathology, Cheng Hsin General Hospital, Taipei, Taiwan, R.O.C.
authors.forename: Jason
authors.initials: J
authors.lastname: Chou
authors.affiliation: National Taiwan University College of Medicine Animal Medicine Center, Taipei, Taiwan, R.O.C.
authors.forename: Ming-Fang
authors.initials: MF
authors.lastname: Wu
authors.affiliation: Department of Pathology and Laboratory Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, R.O.C. School of Medical Laboratory Science and Biotechnology, Taipei Medical University, Taipei, Taiwan, R.O.C. School of Medicine, Fu-Jen Catholic University, New Taipei City, Taiwan, R.O.C.
authors.forename: Yung-Luen
authors.initials: YL
authors.lastname: Shih
authors.affiliation: Office of Director, Cheng Hsin General Hospital, Taipei, Taiwan, R.O.C.
authors.forename: Ming-Yang
authors.initials: MY
authors.lastname: Yeh
authors.affiliation: Jen-Teh Junior College of Medicine, Nursing and Management, Houlong, Taiwan, R.O.C.
authors.forename: Hsueh-Yu
authors.initials: HY
authors.lastname: Chung
authors.affiliation: Department of Clinical Pathology, Cheng Hsin General Hospital, Taipei, Taiwan, R.O.C. School of Medical Laboratory Science and Biotechnology, Taipei Medical University, Taipei, Taiwan, R.O.C.
authors.forename: Hsu-Feng
authors.initials: HF
authors.lastname: Lu
authors.affiliation: Department of Clinical Pathology, Cheng Hsin General Hospital, Taipei, Taiwan, R.O.C.
authors.forename: Nien-Chieh
authors.initials: NC
authors.lastname: Liao
authors.affiliation: Department of Clinical Pathology, Cheng Hsin General Hospital, Taipei, Taiwan, R.O.C.
authors.forename: Jia You
authors.initials: JY
authors.lastname: Liu
authors.affiliation: Department of Clinical Pathology, Cheng Hsin General Hospital, Taipei, Taiwan, R.O.C. jgchung@mail.cmu.edu.tw ch1835@chgh.org.tw.
authors.forename: Shu-Ching
authors.initials: SC
authors.lastname: Hsueh
authors.affiliation: Department of Biological Science and Technology, China Medical University, Taichung Taiwan, R.O.C. Department of Biotechnology, Asia University, Taichung, Taiwan, R.O.C. jgchung@mail.cmu.edu.tw ch1835@chgh.org.tw.
authors.forename: Jing-Gung
authors.initials: JG
authors.lastname: Chung
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000502:physiology; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D059022:Food Safety; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology / Q000628:therapy; D051379:Mice; D009152:Mutagenicity Tests; D025282:Mycelium; D012486:Salmonella typhimurium / Q000235:genetics; D047368:Tumor Burden; D023041:Xenograft Model Antitumor Assays
keywords: Hirsutella sinensis; animal model; food safety; hepatoma; mutagenicity