title: Effects of transforming growth factor-β2 on myocilin expression and secretion in human primary cultured trabecular meshwork cells.
abstract: High intraocular pressure (IOP) is a risk factor for primary open-angle glaucoma (POAG). The trabecular meshwork (TM), a reticular tissue in the outflow passage of the aqueous humor (AH), is a major contributor to intraocular outflow resistance. High levels of myocilin (MYOC), which is expressed in the TM, are associated with high IOP. Furthermore, transforming growth factor-β2 (TGF-β2) concentrations in human AH are significantly elevated in POAG patients. This study was designed to investigate the effects of TGF-β2 on MYOC expression and secretion in human primary cultured TM cells. Primary cultured human TM cells were treated with 0 (control group), 1, 10, and 100 ng/mL TGF-β2 for 12, 24, or 48 h. MYOC mRNA and protein expressions in TM cells and protein secretion in conditioned media were analyzed by semi-quantitative RT-PCR, Western blotting, and enzyme-linked immunosorbent assays (ELISA), respectively. TM cells treated with 1, 10, and, 100 ng/mL TGF-β2 for 48 h showed higher MYOC mRNA and protein expressions than those in the control group (0 ng/mL TGF-β2) (all P < 0.05). Treatment with TGF-β2 for 48 h also induced MYOC secretion in conditioned media in a dose-dependent manner (0 ng/mL: 7.107±1.163 pg/ml; 1 ng/mL: 7.879±1.894 pg/ml; 10 ng/mL: 8.063±1.181 pg/ml; 100 ng/mL: 8.902±0.699 pg/ml; all P < 0.05). In Conclusion, TGF-β2 induced MYOC expression and secretion in human primary cultured TM cells. Further investigations are required to confirm the involvement of these two factors in POAG pathogenesis.
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital of Fujian Medical University Quanzhou, China.
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital of Fujian Medical University Quanzhou, China.
authors.forename: Wanzhu
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital of Fujian Medical University Quanzhou, China.
authors.forename: Maosheng
authors.initials: M
authors.lastname: Guo
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital of Fujian Medical University Quanzhou, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: He
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital of Fujian Medical University Quanzhou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000328:Adult; D015153:Blotting, Western; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000096:biosynthesis* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005136:Eye Proteins / Q000096:biosynthesis* / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis* / Q000378:metabolism; D006801:Humans; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014129:Trabecular Meshwork / Q000378:metabolism*; D053781:Transforming Growth Factor beta2 / Q000378:metabolism*; D000099179:Myocilin
chemical_list: D003598:Cytoskeletal Proteins; D005136:Eye Proteins; D006023:Glycoproteins; D053781:Transforming Growth Factor beta2; D000099179:Myocilin
keywords: Primary cell culture; glaucoma; myocilin; trabecular meshwork cells; transforming growth factor-beta 2