title: Development of microsatellite loci for the riparian tree species Melaleuca argentea (Myrtaceae) using 454 sequencing.
abstract: OBJECTIVE
  Microsatellite primers were developed for Melaleuca argentea (Myrtaceae) to evaluate genetic diversity and population genetic structure of this broadly distributed northern Australian riparian tree species. •
  
  
  RESULTS
  454 GS-FLX shotgun sequencing was used to obtain 5860 sequences containing putative microsatellite motifs. Two multiplex PCRs were optimized to genotype 11 polymorphic microsatellite loci. These loci were screened for variation in individuals from two populations in the Pilbara region, northwestern Western Australia. Overall, observed heterozygosities ranged from 0.27 to 0.86 (mean: 0.52) and the number of alleles per locus ranged from two to 13 (average: 4.3). •
  
  
  CONCLUSIONS
  These microsatellite loci will be useful in future studies of the evolutionary history and population and spatial genetic structure in M. argentea, and inform the development of seed sourcing strategies for the species.
authors.affiliation: Botanic Gardens and Parks Authority, Kings Park and Botanic Garden, West Perth 6005 Western Australia, Australia ; School of Plant Biology, University of Western Australia, Nedlands 6009 Western Australia, Australia.
authors.forename: Paul G
authors.initials: PG
authors.lastname: Nevill
authors.affiliation: Botanic Gardens and Parks Authority, Kings Park and Botanic Garden, West Perth 6005 Western Australia, Australia ; School of Plant Biology, University of Western Australia, Nedlands 6009 Western Australia, Australia.
authors.forename: Anna
authors.initials: A
authors.lastname: Williams
authors.affiliation: Botanic Gardens and Parks Authority, Kings Park and Botanic Garden, West Perth 6005 Western Australia, Australia ; School of Plant Biology, University of Western Australia, Nedlands 6009 Western Australia, Australia.
authors.forename: Siegfried
authors.initials: S
authors.lastname: Krauss
authors.affiliation: Botanic Gardens and Parks Authority, Kings Park and Botanic Garden, West Perth 6005 Western Australia, Australia ; School of Plant Biology, University of Western Australia, Nedlands 6009 Western Australia, Australia.
authors.forename: Donna
authors.initials: D
authors.lastname: Bradbury
authors.affiliation: Rio Tinto Iron Ore, Level 22, 152-158 St. Georges Terrace, Perth 6000 Western Australia, Australia.
authors.forename: Sunil
authors.initials: S
authors.lastname: Samaraweera
authors.affiliation: School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide 5001 South Australia, Australia ; Australian Centre for Evolutionary Biology and Biodiversity, University of Adelaide, Adelaide 5005 South Australia, Australia.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Gardner
keywords: 454 GS-FLX; Melaleuca argentea; northern Australia; nuclear SSR markers; riparian tree; shotgun sequencing