title: Acetylcholine secretion by motor neuron-like cells from umbilical cord mesenchymal stem cells.
abstract: Umbilical cord mesenchymal stem cells were isolated by a double enzyme digestion method. The third passage of umbilical cord mesenchymal stem cells was induced with heparin and/or basic fibroblast growth factor. Results confirmed that cell morphology did not change after induction with basic fibroblast growth factor alone. However, neuronal morphology was visible, and microtubule-associated protein-2 expression and acetylcholine levels increased following induction with heparin alone or heparin combined with basic fibroblast growth factor. Hb9 and choline acetyltransferase expression was high following inductive with heparin combined with basic fibroblast growth factor. Results indicate that the inductive effect of basic fibroblast growth factor alone was not obvious. Heparin combined with basic fibroblast growth factor noticeably promoted the differentiation of umbilical cord mesenchymal stem cells into motor neuron-like cells. Simultaneously, umbilical cord mesenchymal stem cells could secrete acetylcholine.
authors.affiliation: Department of Anatomy, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China.
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Anatomy, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China.
authors.forename: Dehua
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Anatomy, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Department of Anatomy, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Fang
keywords: Hb9; acetylcholine; basic fibroblast growth factor; differentiation; heparin; human umbilical cord mesenchymal stem cell; induction; motor neuron; neural regeneration; neuroregeneration; stem cells