title: Proteome profiling of human cutaneous leishmaniasis lesion.
abstract: In this study, we used proteomics and biological network analysis to evaluate the potential biological processes and components present in the identified proteins of biopsies from cutaneous leishmaniasis (CL) patients infected by Leishmania braziliensis in comparison with normal skin. We identified 59 proteins differently expressed in samples from infected and normal skin. Biological network analysis employing identified proteins showed the presence of networks that may be involved in the cell death mediated by cytotoxic T lymphocytes. After immunohistochemical analyses, the expression of caspase-9, caspase-3, and granzyme B was validated in the tissue and positively correlated with the lesion size in CL patients. In conclusion, this work identified differentially expressed proteins in the inflammatory site of CL, revealed enhanced expression of caspase-9, and highlighted mechanisms associated with the progression of tissue damage observed in lesions.
authors.affiliation: Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz (FIOCRUZ), Salvador, Brazil. Electronic address: cler_ss@hotmail.com.
authors.forename: Claire
authors.initials: C
authors.lastname: da Silva Santos
authors.affiliation: Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Sanaz
authors.initials: S
authors.lastname: Attarha
authors.affiliation: Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Ravi Kanth
authors.initials: RK
authors.lastname: Saini
authors.affiliation: Faculdade de Medicina da Bahia, Universidade Federal da Bahia, Salvador, Brazil.
authors.forename: Viviane
authors.initials: V
authors.lastname: Boaventura
authors.affiliation: Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz (FIOCRUZ), Salvador, Brazil.
authors.forename: Jackson
authors.initials: J
authors.lastname: Costa
authors.affiliation: Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz (FIOCRUZ), Salvador, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Khouri
authors.affiliation: Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz (FIOCRUZ), Salvador, Brazil; Faculdade de Medicina da Bahia, Universidade Federal da Bahia, Salvador, Brazil; Instituto de Investigação em Imunologia, São Paulo, Brazil.
authors.forename: Manoel
authors.initials: M
authors.lastname: Barral-Netto
authors.affiliation: Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz (FIOCRUZ), Salvador, Brazil; Instituto de Investigação em Imunologia, São Paulo, Brazil; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Brazil. Electronic address: brodskyn@bahia.fiocruz.br.
authors.forename: Cláudia Ida
authors.initials: CI
authors.lastname: Brodskyn
authors.affiliation: Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Serhiy
authors.initials: S
authors.lastname: Souchelnytskyi
mesh_terms: D053148:Caspase 3 / Q000032:analysis; D053453:Caspase 9 / Q000032:analysis; D053804:Granzymes / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D016773:Leishmaniasis, Cutaneous / Q000378:metabolism* / Q000473:pathology; D020543:Proteome; D012867:Skin / Q000473:pathology
chemical_list: D020543:Proteome; D053804:Granzymes; D053148:Caspase 3; D053453:Caspase 9