title: Mycobacterium tuberculosis promotes anti-apoptotic activity of the macrophage by PtpA protein-dependent dephosphorylation of host GSK3α.
abstract: Mycobacterium tuberculosis tyrosine phosphatase PtpA inhibits two key cellular events in macrophages required for the elimination of invading organisms, phagosome acidification, and maturation. Kinome analysis revealed multiple PtpA-dependent changes to the phosphorylation status of macrophage proteins upon M. tuberculosis infection. Among those proteins we show that PtpA dephosphorylates GSK3α on amino acid Tyr(279), which leads to modulation of GSK3α anti-apoptotic activity, promoting pathogen survival early during infection.
authors.affiliation: From the Division of Infectious Diseases, Department of Medicine, University of British Columbia, Vancouver, British Columbia V6H 3Z6, Canada.
authors.forename: Valérie
authors.initials: V
authors.lastname: Poirier
authors.affiliation: From the Division of Infectious Diseases, Department of Medicine, University of British Columbia, Vancouver, British Columbia V6H 3Z6, Canada.
authors.forename: Horacio
authors.initials: H
authors.lastname: Bach
authors.affiliation: From the Division of Infectious Diseases, Department of Medicine, University of British Columbia, Vancouver, British Columbia V6H 3Z6, Canada yossi@mail.ubc.ca.
authors.forename: Yossef
authors.initials: Y
authors.lastname: Av-Gay
mesh_terms: D017209:Apoptosis; D001426:Bacterial Proteins / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism*; D006801:Humans; D016676:Macrophages, Alveolar / Q000166:cytology / Q000382:microbiology*; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D010588:Phagosomes / Q000378:metabolism; D010766:Phosphorylation; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D001426:Bacterial Proteins; C414465:MptpA protein, Mycobacterium tuberculosis; D038362:Glycogen Synthase Kinase 3; C448021:glycogen synthase kinase 3 alpha; D017027:Protein Tyrosine Phosphatases; C505407:CASP3 protein, human; D053148:Caspase 3
keywords: Apoptosis; Macrophage; Mycobacterium tuberculosis; Protein-tyrosine Phosphatase (Tyrosine Phosphatase); Signal Transduction