title: Superpulsed (Ga-As, 904 nm) low-level laser therapy (LLLT) attenuates inflammatory response and enhances healing of burn wounds.
abstract: Low-level laser therapy (LLLT) using superpulsed near-infrared light can penetrate deeper in the injured tissue and could allow non-pharmacological treatment for chronic wound healing. This study investigated the effects of superpulsed laser (Ga-As 904 nm, 200 ns pulse width; 100 Hz; 0.7 mW mean output power; 0.4 mW/cm(2) average irradiance; 0.2 J/cm(2) total fluence) on the healing of burn wounds in rats, and further explored the probable associated mechanisms of action. Irradiated group exhibited enhanced DNA, total protein, hydroxyproline and hexosamine contents compared to the control and silver sulfadiazine (reference care) treated groups. LLLT exhibited decreased TNF-α level and NF-kB, and up-regulated protein levels of VEGF, FGFR-1, HSP-60, HSP-90, HIF-1α and matrix metalloproteinases-2 and 9 compared to the controls. In conclusion, LLLT using superpulsed 904 nm laser reduced the inflammatory response and was able to enhance cellular proliferation, collagen deposition and wound contraction in the repair process of burn wounds. Photomicrographs showing no, absence inflammation and faster wound contraction in LLLT superpulsed (904 nm) laser treated burn wounds as compared to the non-irradiated control and silver sulfadiazine (SSD) ointment (reference care) treated wounds.
authors.affiliation: Defence Institute of Physiology and Allied Sciences, DRDO, Lucknow Road, 110 054, Timarpur, Delhi, India. asheeshgupta2001@gmail.com.
authors.forename: Asheesh
authors.initials: A
authors.lastname: Gupta
authors.affiliation: Defence Institute of Physiology and Allied Sciences, DRDO, Lucknow Road, 110 054, Timarpur, Delhi, India.
authors.forename: Gaurav K
authors.initials: GK
authors.lastname: Keshri
authors.affiliation: Defence Institute of Physiology and Allied Sciences, DRDO, Lucknow Road, 110 054, Timarpur, Delhi, India.
authors.forename: Anju
authors.initials: A
authors.lastname: Yadav
authors.affiliation: Defence Institute of Physiology and Allied Sciences, DRDO, Lucknow Road, 110 054, Timarpur, Delhi, India.
authors.forename: Shefali
authors.initials: S
authors.lastname: Gola
authors.affiliation: Defence Institute of Physiology and Allied Sciences, DRDO, Lucknow Road, 110 054, Timarpur, Delhi, India.
authors.forename: Satish
authors.initials: S
authors.lastname: Chauhan
authors.affiliation: Defence Institute of Physiology and Allied Sciences, DRDO, Lucknow Road, 110 054, Timarpur, Delhi, India.
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Salhan
authors.affiliation: Defence Institute of Physiology and Allied Sciences, DRDO, Lucknow Road, 110 054, Timarpur, Delhi, India.
authors.forename: Shashi
authors.initials: S
authors.lastname: Bala Singh
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D015153:Blotting, Western; D002056:Burns / Q000473:pathology / Q000503:physiopathology / Q000532:radiotherapy*; D004195:Disease Models, Animal; D007249:Inflammation / Q000473:pathology / Q000503:physiopathology / Q000532:radiotherapy*; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D010784:Photomicrography; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D012837:Silver Sulfadiazine / Q000494:pharmacology; D012867:Skin / Q000473:pathology / Q000503:physiopathology / Q000528:radiation effects; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology / Q000528:radiation effects
chemical_list: D000891:Anti-Infective Agents, Local; D014409:Tumor Necrosis Factor-alpha; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat; D020780:Matrix Metalloproteinase 9; C572337:Mmp9 protein, rat; D012837:Silver Sulfadiazine
keywords: Burns; hypoxia inducible factor-1α; low-level laser (light) therapy (LLLT); nuclear factor-kB; photobiomodulation; superpulsed laser (904 nm)