title: Cell cycle-related genes p57kip2, Cdk5 and Spin in the pathogenesis of neural tube defects.
abstract: In the field of developmental neurobiology, accurate and ordered regulation of the cell cycle and apoptosis are crucial factors contributing to the normal formation of the neural tube. Preliminary studies identified several genes involved in the development of neural tube defects. In this study, we established a model of developmental neural tube defects by administration of retinoic acid to pregnant rats. Gene chip hybridization analysis showed that genes related to the cell cycle and apoptosis, signal transduction, transcription and translation regulation, energy and metabolism, heat shock, and matrix and cytoskeletal proteins were all involved in the formation of developmental neural tube defects. Among these, cell cycle-related genes were predominant. Retinoic acid ment caused differential expression of three cell cycle-related genes p57kip2, Cdk5 and Spin, the expression levels of which were downregulated by retinoic acid and upregulated during normal neural tube formation. The results of this study indicate that cell cycle-related genes play an important role in the formation of neural tube defects. P57kip2, Cdk5 and Spin may be critical genes in the pathogenesis of neural tube defects.
authors.affiliation: Department of Neurosurgery, Deyang People's Hospital, Deyang 618000, Sichuan Province, China.
authors.forename: Xinjun
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Neurobiology, the Third Military Medical University of Chinese PLA, Chongqing 400038, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, Sichuan Provincial People's Hospital, Chengdu 610041, Sichuan Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Department of Neurosurgery, Deyang People's Hospital, Deyang 618000, Sichuan Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Neurobiology, the Third Military Medical University of Chinese PLA, Chongqing 400038, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Deyang People's Hospital, Deyang 618000, Sichuan Province, China.
authors.forename: Yangyun
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Neurosurgery, Deyang People's Hospital, Deyang 618000, Sichuan Province, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Long
authors.affiliation: Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Chao
authors.initials: C
authors.lastname: You
keywords: cell cycle; gene chip; neural development; neural regeneration; neural tube defects; neurulation; regeneration; regulatory factor; retinoic acid