title: Data analysis for 16S microbial profiling from different benchtop sequencing platforms.
abstract: Progress in microbial ecology is confounded by problems when evaluating results from different sequencing methodologies. Contrary to existing expectations, here we demonstrate that the same biological conclusion is reached using different NGS technologies when stringent sequence quality filtering and accurate clustering algorithms are applied.
authors.affiliation: Microbiology Department, Universidade Federal de Viçosa, Viçosa, MG 36570-900, Brazil; AgroEcology Department, Rothamsted Research, Harpenden, Herts AL52JQ, United Kingdom. Electronic address: victor.pylro@gmail.com.
authors.forename: Victor S
authors.initials: VS
authors.lastname: Pylro
authors.affiliation: Universidade Federal do Pampa, São Gabriel RS, 97300-000, Brazil. Electronic address: luizroesch@unipampa.edu.br.
authors.forename: Luiz Fernando W
authors.initials: LF
authors.lastname: Roesch
authors.affiliation: Microbiology Department, Universidade Federal de Viçosa, Viçosa, MG 36570-900, Brazil; AgroEcology Department, Rothamsted Research, Harpenden, Herts AL52JQ, United Kingdom.
authors.forename: Daniel K
authors.initials: DK
authors.lastname: Morais
authors.affiliation: AgroEcology Department, Rothamsted Research, Harpenden, Herts AL52JQ, United Kingdom.
authors.forename: Ian M
authors.initials: IM
authors.lastname: Clark
authors.affiliation: AgroEcology Department, Rothamsted Research, Harpenden, Herts AL52JQ, United Kingdom.
authors.forename: Penny R
authors.initials: PR
authors.lastname: Hirsch
authors.affiliation: Microbiology Department, Universidade Federal de Viçosa, Viçosa, MG 36570-900, Brazil.
authors.forename: Marcos R
authors.initials: MR
authors.lastname: Tótola
mesh_terms: D001105:Archaea / Q000145:classification / Q000235:genetics; D001419:Bacteria / Q000145:classification / Q000235:genetics; D001938:Brazil; D019295:Computational Biology / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing; D054892:Metagenome; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D012988:Soil Microbiology
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Alpha diversity; Amplicons; Beta diversity; Microbial community analysis; Next generation sequencing