title: The effect of M-phase stage-dependent kinase inhibitors on inositol 1,4,5-trisphosphate receptor 1 (IP3 R1) expression and localization in pig oocytes.
abstract: At fertilization, inositol 1,4,5-trisphosphate receptor type 1 (IP3 R1) has a crucial role in Ca(2+) release in mammals. Expression levels, localization and phosphorylation of IP3 R1 are important for its function, but it still remains unclear which molecule(s) regulates IP3 R1 behavior in pig oocytes. We examined whether there was a difference in localization of IP3 R1 after in vitro or in vivo maturation of pig oocytes. In mouse oocytes, large clusters of IP3 R1 were formed in the cortex of the oocyte except in a ring-shaped band of cortex adjacent to the spindle. However, no such clusters of IP3 R1 were observed in pig oocytes and there was no difference in its localization between in vitro and in vivo matured oocytes. We next tried to clarify which factor(s) regulates IP3 R1 localization, phosphorylation and expression using M-phase stage-dependent kinase inhibitors. Our results show that treatments with roscovitine (p34(cdc2) kinase inhibitor) or U0126 (mitogen-activated protein kinase inhibitor) did not affect IP3 R1 expression or localization in pig oocytes, although the latter strongly inhibited phosphorylation. However, treatment with BI-2536, an inhibitor of polo-like kinase 1 (Plk1), dramatically decreased the expression level of IP3 R1 in pig oocytes in a dose-dependent manner. From these results, it is suggested that Plk1 is involved in the regulation of IP3 R1 expression in pig oocytes.
authors.affiliation: Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand; Laboratory of Animal Reproduction, Graduate School of Veterinary Sciences, Azabu University, Sagamihara, Japan.
authors.forename: Anucha
authors.initials: A
authors.lastname: Sathanawongs
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Fujiwara
authors.forename: Tsubasa
authors.initials: T
authors.lastname: Kato
authors.forename: Masahiko
authors.initials: M
authors.lastname: Hirose
authors.forename: Maki
authors.initials: M
authors.lastname: Kamoshita
authors.forename: Richard J H
authors.initials: RJ
authors.lastname: Wojcikiewicz
authors.forename: Jan B
authors.initials: JB
authors.lastname: Parys
authors.forename: Junya
authors.initials: J
authors.lastname: Ito
authors.forename: Naomi
authors.initials: N
authors.lastname: Kashiwazaki
mesh_terms: D000818:Animals; D002070:Butadienes / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors / Q000502:physiology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects*; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D009570:Nitriles / Q000494:pharmacology*; D009865:Oocytes / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors / Q000502:physiology*; D011621:Pteridines / Q000494:pharmacology*; D011687:Purines / Q000494:pharmacology*; D000077546:Roscovitine; D013552:Swine / Q000378:metabolism*; D000097869:Polo-Like Kinase 1
chemical_list: D002070:Butadienes; D002118:Calcium; D018797:Cell Cycle Proteins; D053496:Inositol 1,4,5-Trisphosphate Receptors; D009570:Nitriles; D047428:Protein Kinase Inhibitors; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D011621:Pteridines; D011687:Purines; D000077546:Roscovitine; D000097869:Polo-Like Kinase 1; C518477:BI 2536; C113580:U 0126
keywords: 4; 5-trisphosphate receptor; inositol 1; maturation; oocyte; pig; polo-like kinase 1