title: Comparison of two laboratory assays in monitoring the efficacy of different prophylaxis regimens for severe haemophilia.
abstract: BACKGROUND
  The goal of this study was to compare the validity of two laboratory assays, rotation thromboelastometry (ROTEM) and endogenous thrombin potential (ETP), in monitoring and evaluating different prophylactic treatment regimens in patients with severe haemophilia.
  
  
  METHODS
  Twenty adult patients with severe haemophilia were divided into three groups according to treatment regimen with concentrate of factor (F) VIII/IX: full-dose prophylaxis (5 patients), intermediate-dose prophylaxis (5 patients), and on demand treatment (10 patients).
  
  
  RESULTS
  The ROTEM for the group treated with full-dose prophylaxis was significantly lower than ROTEM for the group treated with intermediate-dose prophylaxis (p = 0.025). Among the patients given full-dose prophylaxis, 40% (2 patients) had prolonged ROTEM after 3 months of treatment, while among those given intermediate-dose prophylaxis all patients (100%, 5 patients) had prolonged ROTEM (p = 0.038). The ETP was significantly improved after 3 months of full-dose in comparison with intermediate-dose prophylaxis (p = 0.042).
  
  
  CONCLUSIONS
  ROTEM and ETP are useful laboratory assays for monitoring efficacy of different prophylaxis regimens with concentrate of FVIII/IX in patients with severe haemophilia, helping in making decisions regarding optimal dose-regimen prophylaxis.
authors.forename: Irena
authors.initials: I
authors.lastname: Djunic
authors.forename: Violeta
authors.initials: V
authors.lastname: Dopsaj
authors.forename: Predrag
authors.initials: P
authors.lastname: Miljic
authors.forename: Nebojsa
authors.initials: N
authors.lastname: Savic
authors.forename: Natasa
authors.initials: N
authors.lastname: Milic
authors.forename: Mirjana
authors.initials: M
authors.lastname: Mitrovic
authors.forename: Nada
authors.initials: N
authors.lastname: Suvajdzic-Vukovic
authors.forename: Dragica
authors.initials: D
authors.lastname: Tomin
authors.forename: Ivo
authors.initials: I
authors.lastname: Elezovic
mesh_terms: D000328:Adult; D001780:Blood Coagulation Tests; D016022:Case-Control Studies; D019411:Clinical Laboratory Techniques; D016903:Drug Monitoring / Q000379:methods; D005164:Factor IX / Q000096:biosynthesis; D005169:Factor VIII / Q000096:biosynthesis; D006467:Hemophilia A / Q000097:blood* / Q000517:prevention & control*; D006801:Humans; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D013916:Thrombelastography / Q000379:methods; D013917:Thrombin / Q000096:biosynthesis / Q000737:chemistry; D013997:Time Factors; D055815:Young Adult
chemical_list: C078147:F8 protein, human; D005169:Factor VIII; D005164:Factor IX; D013917:Thrombin