title: Evaluation of genotoxic potential of commonly used organophosphate pesticides in peripheral blood lymphocytes of rats.
abstract: Chlorpyrifos (CPF), methyl parathion (MPT), and malathion (MLT) are among the most extensively used organophosphate (OP) pesticides in India. DNA protein cross-links (DPC) and DNA strand breaks are toxic lesions associated with the mechanism(s) of toxicity of carcinogenic compounds. In the present study, we examined the hypothesis that individual and interactive genotoxic effects of CPF, MPT, and MLT are involved in the formation of DPC and DNA strand break. The DNA strand break was measured by comet assay and expressed as DNA damage index, while DPC estimation was carried out by fluorescence emission assay. The results showed that exposure of rat lymphocytes with CPF, MPT, and MLT caused significantly marked increase in DNA damage and DPC formation in time-dependent manner. MPT caused the highest damage, and these pesticides do not potentiate the toxicity of each other.
authors.affiliation: Department of Pharmacology, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: A
authors.initials: A
authors.lastname: Ojha
authors.affiliation: Department of Pharmacology, All India Institute of Medical Sciences, New Delhi, India yk.ykgupta@gmail.com.
authors.forename: Y K
authors.initials: YK
authors.lastname: Gupta
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004390:Chlorpyrifos / Q000633:toxicity*; D004249:DNA Damage; D007306:Insecticides / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D008294:Malathion / Q000633:toxicity*; D008297:Male; D008743:Methyl Parathion / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity*; D017208:Rats, Wistar
chemical_list: D007306:Insecticides; D009153:Mutagens; D008743:Methyl Parathion; D004390:Chlorpyrifos; D008294:Malathion
keywords: Pesticide toxicology; ecotoxicology; genetic toxicology; human toxicology; occupational toxicology; organophosphates