title: Metabolomics of AS-5 RBC supernatants following routine storage.
abstract: OBJECTIVE
  The safety and efficacy of stored red blood cells (RBCs) transfusion has been long debated due to retrospective clinical evidence and laboratory results, indicating a potential correlation between increased morbidity and mortality following transfusion of RBC units stored longer than 14 days. We hypothesize that storage in Optisol additive solution-5 leads to a unique metabolomics profile in the supernatant of stored RBCs.
  
  
  METHODS
  Whole blood was drawn from five healthy donors, RBC units were manufactured, and prestorage leucoreduced by filtration. Samples were taken on days 1 and 42, the cells removed, and mass spectrometry-based metabolomics was performed.
  
  
  RESULTS
  The results confirmed the progressive impairment of RBC energy metabolism by day 42 with indirect markers of a parallel alteration of glutathione and NADPH homeostasis. Moreover, oxidized pro-inflammatory lipids accumulated by the end of storage.
  
  
  CONCLUSIONS
  The supernatants from stored RBCs may represent a burden to the transfused recipients from a metabolomics standpoint.
authors.affiliation: Department of Biochemistry and Molecular Genetics, School of Medicine University of Colorado Denver, Anschutz Medical Campus, Aurora, CO, USA.
authors.forename: A
authors.initials: A
authors.lastname: D'Alessandro
authors.forename: K C
authors.initials: KC
authors.lastname: Hansen
authors.forename: C C
authors.initials: CC
authors.lastname: Silliman
authors.forename: E E
authors.initials: EE
authors.lastname: Moore
authors.forename: M
authors.initials: M
authors.lastname: Kelher
authors.forename: A
authors.initials: A
authors.lastname: Banerjee
mesh_terms: D001793:Blood Preservation / Q000379:methods*; D017707:Erythrocyte Transfusion / Q000379:methods; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D055442:Metabolome; D012189:Retrospective Studies
keywords: oxidative stress; red blood cell; storage; transfusion medicine