title: Elevated levels of miR-155 in blood and urine from patients with nephrolithiasis.
abstract: BACKGROUND
  Both circulating and urinary miRNAs may represent a potential noninvasive molecular biomarker capable of predicting chronic kidney disease, and, in the present study, we will investigate the serum and urinary levels of miR-155 in patients with nephrolithiasis.
  
  
  METHODS
  Serum and urinary levels of miR-155 are quantified in 60 patients with nephrolithiasis; the result was compared to 50 healthy volunteers. Estimated glomerular filtration (eGFR) was calculated and, by simple regression analysis, the correlations of miR-155/eGFR and miR-155/CRP (C-reactive protein) levels were analyzed as well.
  
  
  RESULTS
  The median levels of serum and urinary levels of miR-155 are significantly higher in nephrolithiasis patients than in controls. eGFR inversely correlates with urinary level of miR-155; CRP positively correlates with urinary miR-155. Urinary level of miR-155 inversely correlates with urinary expression of interleukin- (IL-) 1β, IL-6, and tumor necrosis factor- (TNF-) α and positively correlates with urinary expression of regulated upon activation, normal T-cell expressed, and secreted (RANTES).
  
  
  CONCLUSIONS
  Serum and urinary levels of miR-155 were significantly elevated in patients with nephrolithiasis, and the upregulation of miR-155 was correlated with decline of eGFR and elevation of CRP. Our results suggested that miR-155 might play important roles in the pathophysiology of nephrolithiasis via regulating inflammatory cytokines expression. Further study on the molecular pathogenic mechanism and larger scale of clinical trial are required.
authors.affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.
authors.forename: Yang-Yang
authors.initials: YY
authors.lastname: Hu
authors.affiliation: Department of Otolaryngology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
authors.forename: Wei-Da
authors.initials: WD
authors.lastname: Dong
authors.affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Xu
authors.affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Yao
authors.affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Peng
authors.affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.
authors.forename: Jun-Hua
authors.initials: JH
authors.lastname: Zheng
mesh_terms: D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D005919:Glomerular Filtration Rate; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000652:urine*; D008875:Middle Aged; D053040:Nephrolithiasis / Q000097:blood* / Q000235:genetics / Q000503:physiopathology / Q000652:urine*
chemical_list: D016207:Cytokines; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D002097:C-Reactive Protein