title: Gammaretrovirus mRNA expression is mediated by a novel, bipartite post-transcriptional regulatory element.
abstract: Post-transcriptional regulatory mechanisms of several complex and simple retroviruses and retroelements have been elucidated, with the exception of the gammaretrovirus family. We found that, similar to the other retroviruses, gag gene expression of MuLV and XMRV depends on post-transcriptional regulation mediated via an RNA sequence overlapping the pro-pol open reading frame, termed the Post-Transcriptional Element (PTE). PTE function can be replaced by heterologous RNA export elements, e.g. CTE of simian type D retroviruses. Alternatively, Gag particle production is achieved using an RNA/codon optimized gag gene. PTE function is transferable and can replace HIV Rev-RRE-regulated expression of HIV gag. Analysis of PTE by SHAPE revealed a highly structured RNA comprising seven stem-loop structures, with the 5' and 3' stem-loops forming an essential bipartite signal. MuLV and XMRV PTE share 98% identity and have highly similar RNA structures, with changes mostly located to single-stranded regions. PTE identification strongly suggests that all retroviruses and retroelements share common strategies of post-transcriptional gene regulation to produce Gag. Expression depends on complex RNA structures embedded within retroviral mRNA, in coding regions or the 3' untranslated region. These specific structures serve as recognition signals for either cellular or viral proteins.
authors.affiliation: Human Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA.
authors.forename: Guy R
authors.initials: GR
authors.lastname: Pilkington
authors.affiliation: RT Biochemistry Section, Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702, USA Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, 61-704, Poland.
authors.forename: Katarzyna J
authors.initials: KJ
authors.lastname: Purzycka
authors.affiliation: Human Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA.
authors.forename: Jenifer
authors.initials: J
authors.lastname: Bear
authors.affiliation: RT Biochemistry Section, Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702, USA legrices@mail.nih.gov.
authors.forename: Stuart F J
authors.initials: SF
authors.lastname: Le Grice
authors.affiliation: Human Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA Barbara.felber@nih.gov.
authors.forename: Barbara K
authors.initials: BK
authors.lastname: Felber
mesh_terms: D015967:Gene Expression Regulation, Viral; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008979:Moloney murine leukemia virus / Q000235:genetics* / Q000378:metabolism; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry*; D038621:Regulatory Sequences, Ribonucleic Acid; D058008:Xenotropic murine leukemia virus-related virus / Q000235:genetics* / Q000378:metabolism; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000378:metabolism
chemical_list: D015683:Gene Products, gag; D012333:RNA, Messenger; D012367:RNA, Viral; D038621:Regulatory Sequences, Ribonucleic Acid; D054301:gag Gene Products, Human Immunodeficiency Virus