title: Sequential analysis of oxidative stress markers and vitamin C status in acute bacterial osteomyelitis.
abstract: In bacterial bone infections, excessively formed oxidants may result in local and systemic oxidative stress. Vitamin C is the major extracellular nonenzymatic antioxidant, also implicated in bone cells metabolism and viability. The physiological functions of vitamin C largely depend on its redox status. We sequentially assessed oxidative stress markers, hydroperoxides and malondialdehyde (MDA), total antioxidant activity (AOA), total vitamin C, ascorbic acid (Asc), and oxidized/reduced vitamin C ratio in 137 patients with acute osteomyelitis (OM). Compared to 52 healthy controls, in OM group baseline serum hydroperoxides, MDA and oxidized/reduced vitamin C ratio were higher whilst Asc and AOA were lower (P < 0.05, resp.). On the other side, total vitamin C levels in patients and controls were similar (P > 0.05), thereby suggesting a relative rather than absolute vitamin C deficiency in OM. During the follow-up, oxidative stress markers, AOA, and oxidizedreduced vitamin C ratio were gradually returned to normal, while there was no apparent change of total vitamin C concentrations. Persistently high values of oxidized/reduced vitamin C ratio and serum MDA were found in subacute OM. In conclusion, acute OM was associated with enhanced systemic oxidative stress and the shift of vitamin C redox status towards oxidized forms.
authors.affiliation: Clinics for General and Orthopedic Surgery, Medical Faculty Pristina, Anri Dinana bb, 38220 Kosovska Mitrovica, Serbia.
authors.forename: Rade
authors.identifier: 0000-0002-3344-5837
authors.initials: R
authors.lastname: Grbic
authors.affiliation: Institute of Biochemistry, Medical Faculty Pristina, Anri Dinana bb, 38220 Kosovska Mitrovica, Serbia.
authors.forename: Dijana J
authors.initials: DJ
authors.lastname: Miric
authors.affiliation: Institute of Biochemistry, Medical Faculty Pristina, Anri Dinana bb, 38220 Kosovska Mitrovica, Serbia.
authors.forename: Bojana
authors.initials: B
authors.lastname: Kisic
authors.affiliation: Institute of Pathophysiology, Medical Faculty Pristina, Anri Dinana bb, 38220 Kosovska Mitrovica, Serbia.
authors.forename: Ljiljana
authors.identifier: 0000-0003-1165-9022
authors.initials: L
authors.lastname: Popovic
authors.affiliation: Institute of Physiology, Medical Faculty Pristina, Anri Dinana bb, 38220 Kosovska Mitrovica, Serbia.
authors.forename: Vojkan
authors.identifier: 0000-0003-0751-8163
authors.initials: V
authors.lastname: Nestorovic
authors.affiliation: Clinics for General and Orthopedic Surgery, Medical Faculty Pristina, Anri Dinana bb, 38220 Kosovska Mitrovica, Serbia.
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Vasic
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000097:blood*; D015415:Biomarkers / Q000097:blood*; D002648:Child; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde; D010019:Osteomyelitis / Q000097:blood*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid