title: ABCG2 regulates the pattern of self-renewing divisions in cisplatin-resistant non-small cell lung cancer cell lines.
abstract: Overexpression of ABCG2 is considered a major mechanism of cancer drug resistance. Recent studies have shown that ABCG2 can regulate the switch between symmetric and asymmetric cell division in adult stem cells; however, the relationship between ABCG2 and cell division in drug-resistant cancer cells remains to be determined. In the present study, we demonstrated that ABCG2 is involved in the cell division of drug-resistant cancer cells. We first established drug-resistant H460 and A549 cell lines by repeated exposure to cisplatin and found that the expression of ABCG2 in these cell lines was significantly increased. As evidenced by PKH-26 staining, these drug-resistant cell lines favored symmetric division, which differed from the asymmetric division of the parental cells. Furthermore, we established stable ABCG2‑overexpressing and stable shRNA-ABCG2‑knockdown cell lines to evaluate the potential role of ABCG2 in cancer cell division. The results showed that overexpression of ABCG2 in A549 parental cells significantly increased the proportion of symmetric division, whereas knockdown of ABCG2 in drug-resistant A549 cells significantly increased the proportion of asymmetric division. Taken together, our findings suggest that ABCG2 is involved in the modulation of cancer drug resistance by regulating the pattern of cell division. The present study provides novel insight into the role of ABCG2 in cancer treatment resistance.
authors.affiliation: Department of Oncology, Xinqiao Hospital, Third Military Medical University, Chongqing 400038, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Orthopedics, Chinese PLA Beijing Army General Hospital, Beijing 100700, P.R. China.
authors.forename: Jingming
authors.initials: J
authors.lastname: Hou
authors.affiliation: Department of Oncology, Xinqiao Hospital, Third Military Medical University, Chongqing 400038, P.R. China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Oncology, Xinqiao Hospital, Third Military Medical University, Chongqing 400038, P.R. China.
authors.forename: Zongchang
authors.initials: Z
authors.lastname: Song
authors.affiliation: Department of Oncology, Xinqiao Hospital, Third Military Medical University, Chongqing 400038, P.R. China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Oncology, Xinqiao Hospital, Third Military Medical University, Chongqing 400038, P.R. China.
authors.forename: Cui
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Oncology, Xinqiao Hospital, Third Military Medical University, Chongqing 400038, P.R. China.
authors.forename: Wenying
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Oncology, Xinqiao Hospital, Third Military Medical University, Chongqing 400038, P.R. China.
authors.forename: Yide
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Institute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing 400038, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism; D002455:Cell Division; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D055785:Gene Knockdown Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C116614:ABCG2 protein, human; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; D000970:Antineoplastic Agents; D009363:Neoplasm Proteins; D002945:Cisplatin