title: Characterization of swine leucocyte antigen alleles in a crossbred pig to be used in xenotransplant studies.
abstract: We have characterized swine leucocyte antigen (SLA) classes I and II molecules of a domestic pig as a model for use in our xenotransplant program. Molecular characterization of the SLA classes I and II genes is critical to understanding the adaptive immune responses between swine and humans in the event of xenotransplantation. Seven swine leucocyte antigen genes (SLA-1, SLA-2, SLA-3, DQB1, DRB1, DQA and DRA) were analyzed and 15 alleles were identified. A novel DRA*w04re01 is reported for this limited polymorphic class II gene. The heterozygous haplotypes, Hp-32.0/35.0 and Hp-0.13/0.23 were deduced for our IU-pig model, for SLA classes I and II regions, respectively.
authors.affiliation: Indiana University School of Medicine, Department of Surgery, Indianapolis, IN, USA.
authors.forename: L M
authors.initials: LM
authors.lastname: Reyes
authors.forename: R J
authors.initials: RJ
authors.lastname: Blosser
authors.forename: R F
authors.initials: RF
authors.lastname: Smith
authors.forename: A C
authors.initials: AC
authors.lastname: Miner
authors.forename: L L
authors.initials: LL
authors.lastname: Paris
authors.forename: R L
authors.initials: RL
authors.lastname: Blankenship
authors.forename: M F
authors.initials: MF
authors.lastname: Tector
authors.forename: A J
authors.initials: AJ
authors.lastname: Tector
mesh_terms: D056704:Adaptive Immunity; D000483:Alleles; D000818:Animals; D003433:Crosses, Genetic; D006239:Haplotypes; D006579:Heterozygote; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D013552:Swine / Q000235:genetics* / Q000276:immunology
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II
keywords: Sus scrofa; genotyping; haplotypes; polymorphism; swine leucocyte antigen; xenotransplantation