title: Polymorphisms in genes coding for the NK-cell receptor NKG2D and its ligand MICA in recurrent miscarriage.
abstract: OBJECTIVE
  To investigate the possible association of Natural Killer Group (NKG) receptors gene polymorphisms and MHC class I chain-related protein A (MICA) gene polymorphism with recurrent miscarriage (RM).
  
  
  METHODS
  Seven SNPs in NKG2D gene (rs1049174, rs2255336, rs2617160, rs2617161, rs2246809, rs2617169, and rs2617170), one SNP in NKG2A gene (rs1983526), and one SNP in MICA gene (MICA129) were assessed by allelic discrimination (real-time PCR) in both patients and control women.
  
  
  RESULTS
  The rs2617170 T/T genotype significantly protected against RM [OR (95%) = 0.63 (0.40-0.98)]. The NKG2D haplotypes analysis on the basis of pairwise LD revealed two haplotype blocks. In block1, we found an increased frequency of CAT (Pc = 0.007; OR = 2.13; 95% CI = 1.24-3.68) and GGA haplotypes (Pc = 0.041; OR = 2.02; 95%CI = 1.03-3.96) and reduced frequency of CAA haplotype (Pc = 0.027; OR = 0.72; 95% CI = 0.54-0.96) in patients. In block2, increased frequency of GATG haplotype (Pc = 10(-4) ; OR = 9.25; 95% CI = 3.04-28.12) and reduced frequency of ATTC haplotype (Pc = 0.035; OR = 0.69; 95%CI = 0.50-0.97) were seen in patients.
  
  
  CONCLUSIONS
  The NKG2D gene polymorphisms may influence the success of pregnancy in Tunisian women.
authors.affiliation: Research Unit of Hematological and Autoimmune Diseases, Faculty of Pharmacy, University of Monastir, Monastir, Tunisia.
authors.forename: Sondes
authors.initials: S
authors.lastname: Hizem
authors.forename: Nabil
authors.initials: N
authors.lastname: Mtiraoui
authors.forename: Safia
authors.initials: S
authors.lastname: Massaoudi
authors.forename: Catherine
authors.initials: C
authors.lastname: Fortier
authors.forename: Wahid
authors.initials: W
authors.lastname: Boukouaci
authors.forename: Amokrane
authors.initials: A
authors.lastname: Kahina
authors.forename: Dominique
authors.initials: D
authors.lastname: Charron
authors.forename: Touhami
authors.initials: T
authors.lastname: Mahjoub
authors.forename: Ryad
authors.initials: R
authors.lastname: Tamouza
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics*; D000328:Adult; D004252:DNA Mutational Analysis; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D055655:NK Cell Lectin-Like Receptor Subfamily K / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy
chemical_list: D015395:Histocompatibility Antigens Class I; C527085:KLRK1 protein, human; C099187:MHC class I-related chain A; D055655:NK Cell Lectin-Like Receptor Subfamily K
keywords: Haplotypes; MICA gene; NKG gene; polymorphisms; recurrent miscarriage