title: Analgesic, antibacterial and central nervous system depressant activities of Albizia procera leaves.
abstract: OBJECTIVE
  To ascertain analgesic, antibacterial and central nervous system (CNS) depressant activities of ethyl acetate, dichloromethane and carbon tetrachloride fractions of methanol extract of Albizia procera (A. procera) leaves.
  
  
  METHODS
  Leaves extracts of A. procera were tested for analgesic activity by acetic acid induced and formalin test method in mice. The in vitro antibacterial activity was performed by agar well diffusion method. CNS depressant activity was evaluated by hole cross and open field tests.
  
  
  RESULTS
  All the extracts at 200 mg/kg exhibited significant (P<0.01) analgesic activity in acetic acid induced and formalin tests method in mice. Analgesic activity of the ethyl acetate fraction was almost same like as standard drug indomethacin in acetic acid induced method. The CNS depressant activity of the extracts at 500 mg/kg was comparable to the positive control diazepam as determined by hole cross and open field test method. The extracts exhibited moderate antimicrobial activity against all the tested microorganisms (Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, Esherichia coli, Shigella soneii, Shigella boydii) at concentration of 0.8 mg/disc. The measured diameter of zone of inhibition for the extracts was within the range of 7 to 12 mm which was less than the standard kanamycin (16-24 mm).
  
  
  CONCLUSIONS
  It is concluded that all the extracts possess potential analgesic and CNS depressants activity. This study also showed that different fractions of methanol extract could be potential sources of new antimicrobial agents.
authors.affiliation: Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh.
authors.forename: Mst Mahfuza
authors.initials: MM
authors.lastname: Khatoon
authors.affiliation: Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh.
authors.forename: Mst Hajera
authors.initials: MH
authors.lastname: Khatun
authors.affiliation: Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh.
authors.forename: Md Ekramul
authors.initials: ME
authors.lastname: Islam
authors.affiliation: Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh.
authors.forename: Mst Shahnaj
authors.initials: MS
authors.lastname: Parvin
keywords: Albizia procera leaves; Analgesic; Antimicrobial; CNS depressants