title: Microsatellite primers in the lichen symbiotic alga Trebouxia decolorans (Trebouxiophyceae).
abstract: OBJECTIVE
  Polymorphic microsatellite markers were developed for the symbiotic green alga Trebouxia decolorans to study fine-scale population structure and clonal diversity. •
  
  
  RESULTS
  Using Illumina pyrosequencing, 20 microsatellite primer sets were developed for T. decolorans. The primer sets were tested on 43 individuals sampled from four subpopulations in Germany. The primers amplified di-, tri-, and tetranucleotide repeats with three to 15 alleles per locus, and the unbiased haploid diversity per locus ranged from 0.636 to 0.821. •
  
  
  CONCLUSIONS
  The identified microsatellite markers will be useful to study the genetic diversity, dispersal, and reproductive mode of this common lichen photobiont.
authors.affiliation: Biodiversity and Climate Research Centre (BiK-F), Senckenberg Gesellschaft für Naturforschung, 60325 Frankfurt am Main, Germany.
authors.forename: Francesco
authors.initials: F
authors.lastname: Dal Grande
authors.affiliation: Department of Lichenology and Bryology, Botanische Staatssammlung München, 80638 Munich, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Beck
authors.affiliation: Biodiversity and Conservation Biology, WSL Swiss Federal Research Institute, Zürcherstrasse 111, 8903 Birmensdorf, Switzerland.
authors.forename: Garima
authors.initials: G
authors.lastname: Singh
authors.affiliation: Biodiversity and Climate Research Centre (BiK-F), Senckenberg Gesellschaft für Naturforschung, 60325 Frankfurt am Main, Germany ; Institute of Ecology, Evolution and Diversity, Goethe Universität, Max-von-Laue-Str. 13, D-60438 Frankfurt, Germany.
authors.forename: Imke
authors.initials: I
authors.lastname: Schmitt
keywords: Anaptychia ciliaris; Xanthoria parietina; photobiont; population genetics; selectivity; symbiosis