title: Genetic diversity of androdioecious Osmanthus fragrans (Oleaceae) cultivars using microsatellite markers.
abstract: OBJECTIVE
  For cultivar classification, identification, and genetic improvement, microsatellite markers were developed to analyze the genetic diversity of androdioecious Osmanthus fragrans cultivars. •
  
  
  RESULTS
  Fifteen microsatellite markers were developed from sequences downloaded from the National Center for Biotechnology Information, which included two with null alleles. These primers were screened on 62 typical androdioecious O. fragrans cultivars belonging to four groups (Asiaticus, Albus, Luteus, and Aurantiacus). The number of alleles ranged from two to six, with a mean of 3.7 per locus. The observed and expected heterozygosities ranged from 0.1000 to 0.9091 and from 0.1287 to 0.9167, respectively. Results from structure analyses indicated that Asiaticus and Albus were genetically mixed, and Luteus and Aurantiacus were partially genetically differentiated. •
  
  
  CONCLUSIONS
  These markers will be useful for genetic study of androdioecious O. fragrans cultivars and facilitate cultivar classification, particularly for the cultivar groups Luteus and Aurantiacus.
authors.affiliation: International Cultivar Registration Center for Osmanthus , Nanjing Forestry University, Nanjing, Jiangsu 210037, People's Republic of China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Duan
authors.affiliation: International Cultivar Registration Center for Osmanthus , Nanjing Forestry University, Nanjing, Jiangsu 210037, People's Republic of China.
authors.forename: Xianrong
authors.initials: X
authors.lastname: Wang
authors.affiliation: International Cultivar Registration Center for Osmanthus , Nanjing Forestry University, Nanjing, Jiangsu 210037, People's Republic of China.
authors.forename: Qibai
authors.initials: Q
authors.lastname: Xiang
authors.affiliation: International Cultivar Registration Center for Osmanthus , Nanjing Forestry University, Nanjing, Jiangsu 210037, People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: Liang
authors.affiliation: International Cultivar Registration Center for Osmanthus , Nanjing Forestry University, Nanjing, Jiangsu 210037, People's Republic of China.
authors.forename: Xuexia
authors.initials: X
authors.lastname: Li
authors.affiliation: International Cultivar Registration Center for Osmanthus , Nanjing Forestry University, Nanjing, Jiangsu 210037, People's Republic of China.
authors.forename: Yulian
authors.initials: Y
authors.lastname: Liu
authors.affiliation: International Cultivar Registration Center for Osmanthus , Nanjing Forestry University, Nanjing, Jiangsu 210037, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
keywords: Oleaceae; Osmanthus fragrans; androdioecy; genetic diversity; microsatellite markers