title: A three generation reproduction study with Sprague-Dawley rats consuming high-amylose transgenic rice.
abstract: The transgenic rice line (TRS) enriched with amylose and resistant starch (RS) was developed by antisense RNA inhibition of starch-branching enzymes. Cereal starch with high amylose has a great benefit on human health through its resistant starch. In order to evaluate the effect of transgenic rice on rats, the rats were fed diets containing 70% TRS rice flour, its near-isogenic rice flour or the standard diet as the control through three generations. In the present study, clinical performance, reproductive capacity and pathological responses including body weight, food consumption, reproductive data, hematological parameters, serum chemistry components, organ relative weights and histopathology were examined. Some statistically significant differences were observed in rats consuming the high amylose rice diet when compared to rats fed the near-isogenic control rice diet or the conventional (non-rice) standard diet. These differences were generally of small magnitude, appeared to be random in nature, and were within normal limits for the strain of rat used, and were therefore not considered to be biologically meaningful or treatment related.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Xing Hua
authors.initials: XH
authors.lastname: Zhou
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: ydong@ujs.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Dong
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Yan Sheng
authors.initials: YS
authors.lastname: Zhao
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Xiao
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Yuan Qing
authors.initials: YQ
authors.lastname: He
authors.affiliation: Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China. Electronic address: qqliu@yzu.edu.cn.
authors.forename: Qiao Quan
authors.initials: QQ
authors.lastname: Liu
mesh_terms: D000688:Amylose / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004032:Diet; D004435:Eating / Q000187:drug effects; D005260:Female; D005527:Food, Fortified / Q000009:adverse effects / Q000032:analysis; D008297:Male; D012275:Oryza / Q000009:adverse effects / Q000737:chemistry / Q000235:genetics; D010053:Ovary / Q000187:drug effects / Q000473:pathology; D030821:Plants, Genetically Modified / Q000009:adverse effects / Q000737:chemistry / Q000235:genetics; D017207:Rats, Sprague-Dawley; D012098:Reproduction / Q000187:drug effects*; D013737:Testis / Q000187:drug effects / Q000473:pathology
chemical_list: D000688:Amylose
keywords: High-amylose rice; Reproductive toxicology; Safety; Three-generation study; Transgenic rice