title: Crystallization and preliminary X-ray crystallographic analysis of the PH-GRAM domain of human MTMR4.
abstract: Phosphoinositide lipid molecules play critical roles in intracellular signalling pathways and are regulated by phospholipases, lipid kinases and phosphatases. In particular, phosphatidylinositol 3-phosphate and phosphatidylinositol 3,5-bisphosphate are related to endosomal trafficking events through the recruitment of effector proteins and are involved in the degradation step of autophagy. Myotubularin-related proteins (MTMRs) are a large family of phosphatases that catalyze the dephosphorylation of phosphatidylinositol 3-phosphate and phosphatidylinositol 3,5-bisphosphate at the D3 position, thereby regulating cellular phosphoinositide levels. In this study, the PH-GRAM domain of human MTMR4 was cloned, overexpressed in Escherichia coli, purified and crystallized by the vapour-diffusion method. The crystals diffracted to 3.20 Å resolution at a synchrotron beamline and belonged to either space group P61 or P65, with unit-cell parameters a = b = 109.10, c = 238.97 Å.
authors.affiliation: Department of Chemistry, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Jee Un
authors.initials: JU
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Son
authors.affiliation: Department of Chemistry, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Ki-Young
authors.initials: KY
authors.lastname: Yoo
authors.affiliation: Department of Chemistry, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Woori
authors.initials: W
authors.lastname: Shin
authors.affiliation: Department of Chemistry, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Dong-Won
authors.initials: DW
authors.lastname: Im
authors.affiliation: Medical Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, 111 Gwahangno, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Seung Jun
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Bio Engineering, Hanyang University, Seongdong-gu, Seoul 133-791, Republic of Korea.
authors.forename: Seong Eon
authors.initials: SE
authors.lastname: Ryu
authors.affiliation: Department of Chemistry, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Yong-Seok
authors.initials: YS
authors.lastname: Heo
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D011487:Protein Conformation; D054558:Protein Tyrosine Phosphatases, Non-Receptor / Q000737:chemistry*
chemical_list: D017931:DNA Primers; C427064:MTMR4 protein, human; D054558:Protein Tyrosine Phosphatases, Non-Receptor
keywords: MTMR4; PH-GRAM domain; myotubularin-related proteins; phosphatase; phosphoinositide