title: In Vitro Effects of Ivermectin and Sulphadiazine on Toxoplasma gondii.
abstract: OBJECTIVE
  Ivermectin and sulphadiazine were tested individually to determine their in vitro effects on Toxoplasma gondii grown in human epidermoid larynx carcinoma (Hep-2) cell culture.
  
  
  METHODS
  In-vitro study.
  
  
  METHODS
  Toxoplasma growth was quantities by an enzyme immunoassay performed directly on the fixed cultures, using a rabbit anti-T. gondii immunoglobulin G as the first antibody and a phosphatase-labeled anti-rabbit immunoglobulin G as the second antibody. For each drug, regression models were used to quantify the relationship between optical density values and antimicrobial agent concentrations in the cultures.
  
  
  RESULTS
  The 50% inhibitory concentrations (IC50) of ivermectin and sulphadiazine were found to be 0.2 μg/mL and 7.3 μg/mL after 48 h of exposure, respectively. None of the concentrations tested for each drugs demonstrated toxicity to Hep-2 cells after 72 h of incubation.
  
  
  CONCLUSIONS
  These results indicate that ivermectin significantly inhibited replication of the tachyzoites of T. gondii RH strain.
authors.affiliation: Microbiology Laboratory, Samsun Education and Research Hospital, Samsun, Turkey.
authors.forename: Melek
authors.initials: M
authors.lastname: Bilgin
authors.affiliation: Department of Biology, Faculty of Arts and Sciences, Amasya University, Amasya, Turkey.
authors.forename: Tuba
authors.initials: T
authors.lastname: Yıldırım
authors.affiliation: Department of Medical Microbiology, Cerrahpaşa Faculty of Medicine, İstanbul University, İstanbul, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Hökelek
keywords: Hep-2; Toxoplasma gondii; in vitro; ivermectin; sulphadiazine