title: One step preparation and electrochemical analysis of IQS, a cell-cell communication signal in the nosocomial pathogen Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa uses a hierarchical cell-cell communication system consisting of a number of regulatory elements to coordinate the expression of bacterial virulence genes. Sensitive detection of quorum sensing (QS) molecules has the potential for early identification of P. aeruginosa facilitating early medical intervention. A recently isolated cell-cell communication molecule, a thiazole termed IQS, can bypass the las QS system of P. aeruginosa under times of stress, activating a subset of QS-controlled genes. This compound offers a new target for pathogen detection and has been prepared in a one step protocol. A simple electrochemical strategy was employed for its sensitive detection using boron-doped diamond and glassy carbon electrodes by cyclic voltammetry and amperometry.
authors.affiliation: Innovative Chromatography Group, Irish Separation Science Cluster (ISSC), Ireland; Department of Chemistry and Analytical & Biological Chemistry Research Facility (ABCRF), University College Cork, Ireland.
authors.forename: Fengjun
authors.initials: F
authors.lastname: Shang
authors.affiliation: Department of Chemistry and Analytical & Biological Chemistry Research Facility (ABCRF), University College Cork, Ireland.
authors.forename: Eoin Ó
authors.initials: EÓ
authors.lastname: Muimhneacháin
authors.affiliation: BIOMERIT Research Centre, Department of Microbiology, University College Cork, Ireland.
authors.forename: F
authors.initials: F
authors.lastname: Jerry Reen
authors.affiliation: Innovative Chromatography Group, Irish Separation Science Cluster (ISSC), Ireland; Department of Chemistry and Analytical & Biological Chemistry Research Facility (ABCRF), University College Cork, Ireland.
authors.forename: Alyah
authors.initials: A
authors.lastname: Buzid
authors.affiliation: BIOMERIT Research Centre, Department of Microbiology, University College Cork, Ireland; Curtin University, School of Biomedical Sciences, Perth, WA 6845, Australia.
authors.forename: Fergal
authors.initials: F
authors.lastname: O'Gara
authors.affiliation: Innovative Chromatography Group, Irish Separation Science Cluster (ISSC), Ireland; Department of Chemistry and Analytical & Biological Chemistry Research Facility (ABCRF), University College Cork, Ireland.
authors.forename: John H T
authors.initials: JHT
authors.lastname: Luong
authors.affiliation: Innovative Chromatography Group, Irish Separation Science Cluster (ISSC), Ireland; Department of Chemistry and Analytical & Biological Chemistry Research Facility (ABCRF), University College Cork, Ireland.
authors.forename: Jeremy D
authors.initials: JD
authors.lastname: Glennon
authors.affiliation: Department of Chemistry and Analytical & Biological Chemistry Research Facility (ABCRF), University College Cork, Ireland.
authors.forename: Gerard P
authors.initials: GP
authors.lastname: McGlacken
mesh_terms: D002244:Carbon / Q000737:chemistry; D002450:Cell Communication / Q000235:genetics*; D055664:Electrochemical Techniques; D004566:Electrodes; D015394:Molecular Structure; D011550:Pseudomonas aeruginosa / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D053038:Quorum Sensing / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics*; D013844:Thiazoles / Q000737:chemistry*
chemical_list: D013844:Thiazoles; D002244:Carbon