title: Induction of potential protective immunity against enterotoxemia in calves by single or multiple recombinant Clostridium perfringens toxoids.
abstract: Cattle enterotoxemia caused by Clostridium perfringens toxins is a noncontagious, sporadic, and fatal disease characterized by sudden death. Strategies for controlling and preventing cattle enterotoxemia are based on systematic vaccination of herds with toxoids. Because the process of producing conventional clostridial vaccines is dangerous, expensive, and time-consuming, the prospect of recombinant toxoid vaccines against diseases caused by C. perfringens toxins is promising. In this study, nontoxic recombinant toxoids derived from α-, β- and ε-toxins of C. perfringens, namely, rCPA247-370 , rCPB and rEtxHP, respectively, were expressed in Escherichia coli. High levels of specific IgG antibodies and neutralizing antibodies against the toxins were detected in sera from calves vaccinated with either a single recombinant toxoid or a mixed cocktail of all three recombinant toxoids, indicating the potential of these recombinant toxoids to provide calves with protective immunity against enterotoxemia caused by C. perfringens.
authors.affiliation: Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427, Maduan Street, Harbin, 150001, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Jiang
authors.forename: Yanyan
authors.initials: Y
authors.lastname: De
authors.forename: Jitao
authors.initials: J
authors.lastname: Chang
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Yu
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D057134:Antibodies, Neutralizing / Q000097:blood; D000992:Antitoxins / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D003015:Clostridium Infections / Q000517:prevention & control / Q000662:veterinary*; D003016:Clostridium perfringens / Q000235:genetics / Q000276:immunology*; D004767:Enterotoxemia / Q000517:prevention & control*; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D015870:Gene Expression; D007074:Immunoglobulin G / Q000097:blood; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D014121:Toxoids / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000907:Antibodies, Bacterial; D057134:Antibodies, Neutralizing; D000992:Antitoxins; C035454:Clostridium perfringens toxoid; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014121:Toxoids
keywords: Clostridium perfringens; calf; enterotoxemia; neutralizing antibody; recombinant toxoid