title: Proteomic profiling of serum from patients with tuberculosis.
abstract: BACKGROUND
  Effective treatment and monitoring of tuberculosis (TB) requires biomarkers that can be easily evaluated in blood samples. The aim of this study was to analyze the serum proteome of patients with TB and to identify protein biomarkers for TB.
  
  
  METHODS
  Serum samples from 26 TB patients and 31 controls were analyzed by using nano-flow ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry in data-independent mode, and protein and peptide amounts were calculated by using a label-free quantitative approach. The generated data were analyzed by using principal component analysis and partial least squares discriminant analysis, a multivariate statistical method.
  
  
  RESULTS
  Of more than 500 proteins identified, alpha-1-antitrypsin was the most discriminative, which was 4.4 times higher in TB patients than in controls. Peptides from alpha-1-antitrypsin and antithrombin III increased in TB patients and showed a high variable importance in the projection scores and coefficient in partial least square discriminant analysis.
  
  
  CONCLUSIONS
  Sera from patients with TB had higher alpha-1-antitrypsin levels than sera from control participants. Alpha-1-antitrypsin levels may aid in the diagnosis of TB.
authors.affiliation: Clinical Proteomics Laboratory, Seoul National University Hospital, Seoul, Korea. ; Biomedical Research Institute and Department of Laboratory Medicine, Seoul National University Hospital, Seoul, Korea.
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Song
authors.affiliation: Clinical Proteomics Laboratory, Seoul National University Hospital, Seoul, Korea. ; Biomedical Research Institute and Department of Laboratory Medicine, Seoul National University Hospital, Seoul, Korea.
authors.forename: Minje
authors.initials: M
authors.lastname: Han
authors.affiliation: Clinical Proteomics Laboratory, Seoul National University Hospital, Seoul, Korea.
authors.forename: Yang Seon
authors.initials: YS
authors.lastname: Choi
authors.affiliation: Clinical Proteomics Laboratory, Seoul National University Hospital, Seoul, Korea.
authors.forename: Ki Soon
authors.initials: KS
authors.lastname: Dan
authors.affiliation: Clinical Proteomics Laboratory, Seoul National University Hospital, Seoul, Korea.
authors.forename: Man Gil
authors.initials: MG
authors.lastname: Yang
authors.affiliation: Department of Laboratory Medicine, Seoul National University Bundang Hospital, Seongnam, Korea.
authors.forename: Junghan
authors.initials: J
authors.lastname: Song
authors.affiliation: Clinical Proteomics Laboratory, Seoul National University Hospital, Seoul, Korea. ; Biomedical Research Institute and Department of Laboratory Medicine, Seoul National University Hospital, Seoul, Korea.
authors.forename: Sung Sup
authors.initials: SS
authors.lastname: Park
authors.affiliation: Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea.
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000328:Adult; D000368:Aged; D000990:Antithrombin III / Q000032:analysis; D015415:Biomarkers / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D016002:Discriminant Analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014376:Tuberculosis / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D000515:alpha 1-Antitrypsin / Q000032:analysis
chemical_list: D015415:Biomarkers; D020543:Proteome; D000515:alpha 1-Antitrypsin; D000990:Antithrombin III
keywords: Alpha-1-antitrypsin; Proteomics; Tuberculosis