title: Kinetic model for a threshold filter in an enzymatic system for bioanalytical and biocomputing applications.
abstract: A recently experimentally observed biochemical "threshold filtering" mechanism by processes catalyzed by the enzyme malate dehydrogenase is explained in terms of a model that incorporates an unusual mechanism of inhibition of this enzyme that has a reversible mechanism of action. Experimental data for a system in which the output signal is produced by biocatalytic processes of the enzyme glucose dehydrogenase are analyzed to verify the model's validity. We also establish that fast reversible conversion of the output product to another compound, without the additional inhibition, cannot on its own result in filtering.
authors.affiliation: Department of Physics and ‡Department of Chemistry and Biomolecular Science, Clarkson University , Potsdam, New York 13676, United States.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Privman
authors.forename: Sergii
authors.initials: S
authors.lastname: Domanskyi
authors.forename: Shay
authors.initials: S
authors.lastname: Mailloux
authors.forename: Yaovi
authors.initials: Y
authors.lastname: Holade
authors.forename: Evgeny
authors.initials: E
authors.lastname: Katz
mesh_terms: D055162:Biocatalysis; D015374:Biosensing Techniques; D039301:Computers, Molecular; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007700:Kinetics; D008291:Malate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008954:Models, Biological
chemical_list: D004791:Enzyme Inhibitors; D008291:Malate Dehydrogenase