title: Ischemic preconditioning reduces ischemic brain injury by suppressing nuclear factor kappa B expression and neuronal apoptosis.
abstract: Ischemic stroke induces a series of complex pathophysiological events including blood-brain barrier disruption, inflammatory response and neuronal apoptosis. Previous studies demonstrate that ischemic preconditioning attenuates ischemic brain damage via inhibiting blood-brain barrier disruption and the inflammatory response. Rats underwent transient (15 minutes) occlusion of the bilateral common carotid artery with 48 hours of reperfusion, and were subjected to permanent middle cerebral artery occlusion. This study explored whether ischemic preconditioning could reduce ischemic brain injury and relevant molecular mechanisms by inhibiting neuronal apoptosis. Results found that at 72 hours following cerebral ischemia, myeloperoxidase activity was enhanced, malondialdehyde levels increased, and neurological function was obviously damaged. Simultaneously, neuronal apoptosis increased, and nuclear factor-κB and cleaved caspase-3 expression was significantly increased in ischemic brain tissues. Ischemic preconditioning reduced the cerebral ischemia-induced inflammatory response, lipid peroxidation, and neurological function injury. In addition, ischemic preconditioning decreased nuclear factor-κB p65 and cleaved caspase-3 expression. These results suggested that ischemic preconditioning plays a protective effect against ischemic brain injury by suppressing the inflammatory response, reducing lipid peroxidation, and neuronal apoptosis via inhibition of nuclear factor-κB and cleaved caspase-3 expression.
authors.affiliation: Department of Neurosurgery, Affiliated Union Hospital of Fujian Medical University, Fujian Neurosurgical Institute, Fuzhou 350001, Fujian Province, China.
authors.forename: Songsheng
authors.initials: S
authors.lastname: Shi
authors.affiliation: Department of Neurosurgery, Affiliated Union Hospital of Fujian Medical University, Fujian Neurosurgical Institute, Fuzhou 350001, Fujian Province, China.
authors.forename: Weizhong
authors.initials: W
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, Affiliated Union Hospital of Fujian Medical University, Fujian Neurosurgical Institute, Fuzhou 350001, Fujian Province, China.
authors.forename: Xiankun
authors.initials: X
authors.lastname: Tu
authors.affiliation: Department of Neurosurgery, Affiliated Union Hospital of Fujian Medical University, Fujian Neurosurgical Institute, Fuzhou 350001, Fujian Province, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Affiliated Union Hospital of Fujian Medical University, Fujian Neurosurgical Institute, Fuzhou 350001, Fujian Province, China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Wang
keywords: apoptosis; brain injury; cleaved caspase-3; grants-supported paper; ischemic preconditioning; neural cells; neural regeneration; neuroregeneration; nuclear factor kappa-B; photographs-containing paper