title: Microsatellite markers for the yam bean Pachyrhizus (Fabaceae).
abstract: OBJECTIVE
  Microsatellite loci were developed for the understudied root crop yam bean (Pachyrhizus spp.) to investigate intraspecific diversity and interspecific relationships within the genus Pachyrhizus. •
  
  
  RESULTS
  Seventeen nuclear simple sequence repeat (SSR) markers with perfect di- and trinucleotide repeats were developed from 454 pyrosequencing of SSR-enriched genomic libraries. Loci were characterized in P. ahipa and wild and cultivated populations of four closely related species. All loci successfully cross-amplified and showed high levels of polymorphism, with number of alleles ranging from three to 12 and expected heterozygosity ranging from 0.095 to 0.831 across the genus. •
  
  
  CONCLUSIONS
  By enabling rapid assessment of genetic diversity in three native neotropical crops, P. ahipa, P. erosus, and P. tuberosus, and two wild relatives, P. ferrugineus and P. panamensis, these markers will allow exploration of the genetic diversity and evolutionary history of the genus Pachyrhizus.
authors.affiliation: UMR 7206 Eco-anthropologie et ethnobiologie, Muséum National d'Histoire Naturelle, Département Hommes Natures Sociétés, CP 135, 57 rue Cuvier, 75231 Paris CEDEX 05, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Delêtre
authors.affiliation: UMR 7206 Eco-anthropologie et ethnobiologie, Muséum National d'Histoire Naturelle, Département Hommes Natures Sociétés, CP 135, 57 rue Cuvier, 75231 Paris CEDEX 05, France.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Soengas
authors.affiliation: SSM-UMS 2700 MNHN-CNRS, Muséum National d'Histoire Naturelle, Département Systématique et Evolution, CP 26, 57 rue Cuvier, 75231 Paris CEDEX 05, France.
authors.forename: José
authors.initials: J
authors.lastname: Utge
authors.affiliation: SSM-UMS 2700 MNHN-CNRS, Muséum National d'Histoire Naturelle, Département Systématique et Evolution, CP 26, 57 rue Cuvier, 75231 Paris CEDEX 05, France.
authors.forename: Josie
authors.initials: J
authors.lastname: Lambourdière
authors.affiliation: Plant Systematic Group, Department of Plant and Environmental Sciences, Faculty of Life Sciences, University of Copenhagen, Rolighedsvej 21, DK-1958 Frederiksberg C, Denmark.
authors.forename: Marten
authors.initials: M
authors.lastname: Sørensen
keywords: Fabaceae; Pachyrhizus; cross-species amplification; microsatellites; pyrosequencing; yam bean