title: Unique features of a Japanese 'Candidatus Liberibacter asiaticus' strain revealed by whole genome sequencing.
abstract: Citrus greening (huanglongbing) is the most destructive disease of citrus worldwide. It is spread by citrus psyllids and is associated with phloem-limited bacteria of three species of α-Proteobacteria, namely, 'Candidatus Liberibacter asiaticus', 'Ca. L. americanus', and 'Ca. L. africanus'. Recent findings suggested that some Japanese strains lack the bacteriophage-type DNA polymerase region (DNA pol), in contrast to the Floridian psy62 strain. The whole genome sequence of the pol-negative 'Ca. L. asiaticus' Japanese isolate Ishi-1 was determined by metagenomic analysis of DNA extracted from 'Ca. L. asiaticus'-infected psyllids and leaf midribs. The 1.19-Mb genome has an average 36.32% GC content. Annotation revealed 13 operons encoding rRNA and 44 tRNA genes, but no typical bacterial pathogenesis-related genes were located within the genome, similar to the Floridian psy62 and Chinese gxpsy. In contrast to other 'Ca. L. asiaticus' strains, the genome of the Japanese Ishi-1 strain lacks a prophage-related region.
authors.affiliation: NARO Institute of Fruit Tree Science, Tsukuba, Ibaraki, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Katoh
authors.affiliation: NARO Institute of Fruit Tree Science, Tsukuba, Ibaraki, Japan.
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Miyata
authors.affiliation: Kuchinotsu Citrus Research Station, NARO Institute of Fruit Tree Science, Minami-shimabara, Nagasaki, Japan.
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Inoue
authors.affiliation: NARO Institute of Fruit Tree Science, Tsukuba, Ibaraki, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Iwanami
mesh_terms: D020561:Alphaproteobacteria / Q000235:genetics*; D001120:Arginine / Q000096:biosynthesis; D001435:Bacteriophages / Q000235:genetics; D020029:Base Pairing; D001483:Base Sequence; D053898:Biosynthetic Pathways / Q000235:genetics; D002957:Citrus / Q000382:microbiology; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D005798:Genes, Bacterial; D016680:Genome, Bacterial / Q000235:genetics*; D007564:Japan; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D018515:Plant Leaves / Q000382:microbiology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D001120:Arginine; D004259:DNA-Directed DNA Polymerase