title: New microsatellite loci for Prosopis alba and P. chilensis (Fabaceae).
abstract: OBJECTIVE
  As only six useful microsatellite loci that exhibit broad cross-amplification are so far available for Prosopis species, it is necessary to develop a larger number of codominant markers for population genetic studies. Simple sequence repeat (SSR) markers obtained for Prosopis species from a 454 pyrosequencing run were optimized and characterized for studies in P. alba and P. chilensis. •
  
  
  RESULTS
  Twelve markers that were successfully amplified showed polymorphism in P. alba and P. chilensis. The number of alleles per locus ranged between two and seven and heterozygosity estimates ranged from 0.2 to 0.8. Most of these loci cross-amplify in P. ruscifolia, P. flexuosa, P. kuntzei, P. glandulosa, and P. pallida. •
  
  
  CONCLUSIONS
  These loci will enable genetic diversity studies of P. alba and P. chilensis and contribute to fine-scale population structure, indirect estimation of relatedness among individuals, and marker-assisted selection.
authors.affiliation: Laboratorio de Genética, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina ; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917 (C1033AAJ), Buenos Aires, Argentina.
authors.forename: Cecilia F
authors.initials: CF
authors.lastname: Bessega
authors.affiliation: Laboratorio de Genética, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina ; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917 (C1033AAJ), Buenos Aires, Argentina.
authors.forename: Carolina L
authors.initials: CL
authors.lastname: Pometti
authors.affiliation: Centre for Australian National Biodiversity Research, CSIRO Plant Industry, GPO Box 1600, Canberra, Australian Capital Territory 2601, Australia.
authors.forename: Joe T
authors.initials: JT
authors.lastname: Miller
authors.affiliation: Centre for Australian National Biodiversity Research, CSIRO Plant Industry, GPO Box 1600, Canberra, Australian Capital Territory 2601, Australia.
authors.forename: Richard
authors.initials: R
authors.lastname: Watts
authors.affiliation: Laboratorio de Genética, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina ; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917 (C1033AAJ), Buenos Aires, Argentina.
authors.forename: Beatriz O
authors.initials: BO
authors.lastname: Saidman
authors.affiliation: Laboratorio de Genética, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina ; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917 (C1033AAJ), Buenos Aires, Argentina.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Vilardi
keywords: Fabaceae; Prosopis; microsatellite; next-generation sequencing data