title: Complete genome sequence of the Radiation-Resistant bacterium Rubrobacter radiotolerans RSPS-4.
abstract: Rubrobacter radiotolerans strain RSPS-4 is a slightly thermophilic member of the phylum "Actinobacteria" isolated from a hot spring in São Pedro do Sul, Portugal. This aerobic and halotolerant bacterium is also extremely resistant to gamma and UV radiation, which are the main reasons for the interest in sequencing its genome. Here, we present the complete genome sequence of strain RSPS-4 as well as its assembly and annotation. We also compare the gene sequence of this organism with that of the type strain of the species R. radiotolerans isolated from a hot spring in Japan. The genome of strain RSPS-4 comprises one circular chromosome of 2,875,491 bp with a G+C content of 66.91%, and 3 circular plasmids of 190,889 bp, 149,806 bp and 51,047 bp, harboring 3,214 predicted protein coding genes, 46 tRNA genes and a single rRNA operon.
authors.affiliation: Next Generation Sequencing Unit, Biocant, Biotechnology Innovation Center, Cantanhede, Portugal.
authors.forename: C
authors.initials: C
authors.lastname: Egas
authors.affiliation: Next Generation Sequencing Unit, Biocant, Biotechnology Innovation Center, Cantanhede, Portugal.
authors.forename: C
authors.initials: C
authors.lastname: Barroso
authors.affiliation: Next Generation Sequencing Unit, Biocant, Biotechnology Innovation Center, Cantanhede, Portugal.
authors.forename: H J C
authors.initials: HJ
authors.lastname: Froufe
authors.affiliation: Next Generation Sequencing Unit, Biocant, Biotechnology Innovation Center, Cantanhede, Portugal.
authors.forename: J
authors.initials: J
authors.lastname: Pacheco
authors.affiliation: Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: L
authors.initials: L
authors.lastname: Albuquerque
authors.affiliation: Department of Life Sciences, University of Coimbra, Coimbra, Portugal.
authors.forename: M S
authors.initials: MS
authors.lastname: da Costa
keywords: 454 sequencing; Rubrobacter radiotolerans; genome sequence; gram positive; radiation-resistance