title: Mice deficient in intestinal γδ intraepithelial lymphocytes display an altered intestinal O-glycan profile compared with wild-type littermates.
abstract: Intestinal γδ T-cell receptor-bearing intraepithelial lymphocytes (γδ IELs) play a multifaceted role in maintaining mucosal homeostasis. In order to investigate the relationship between O-glycosylation and inflammation, we carried out an in-depth mass spectrometric comparison of the intestinal O-glycosylation profile of mice lacking γδ IELs (TCRδ(-/-)) and of their wild-type (WT) littermates. A total of 69 nonsulfated and 59 sulfated compositional types of O-glycans were identified in the small intestine and colon of TCRδ(-/-) and WT mice. Our results demonstrated structural differences in intestinal glycosylation in TCRδ(-/-) mice compared with WT littermates. TCRδ(-/-) colons contained a lower proportion of core-2 structures and an increased proportion of core-1 structures whereas TCRδ(-/-) small intestines had a decreased percentage of core-3 structures. The glycan antennae in TCRδ(-/-) colon and small intestine showed altered structural diversity compared with WT mice. There were significant differences in the sialylated species between the TCRδ(-/-) and WT mice with the sialylated Tn antigen found exclusively in the TCRδ(-/-)small intestine, whereas the sulfation pattern remained mostly unchanged. These findings provide novel molecular insights underpinning the role of γδ IELs in maintaining gut homeostasis.
authors.affiliation: Gut Health and Food Safety Institute Strategic Programme, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK.
authors.forename: Christine
authors.initials: C
authors.lastname: Fuell
authors.affiliation: Gut Health and Food Safety Institute Strategic Programme, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK.
authors.forename: Olivia I
authors.initials: OI
authors.lastname: Kober
authors.affiliation: Gut Health and Food Safety Institute Strategic Programme, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Hautefort
authors.affiliation: Gut Health and Food Safety Institute Strategic Programme, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK nathalie.juge@ifr.ac.uk.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Juge
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D003106:Colon / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006031:Glycosylation; D018928:Immunity, Mucosal; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D007421:Intestine, Small / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000172:deficiency / Q000235:genetics* / Q000276:immunology; D012794:Sialic Acids / Q000737:chemistry / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*
chemical_list: D011134:Polysaccharides; D016692:Receptors, Antigen, T-Cell, gamma-delta; D012794:Sialic Acids
keywords: MALDI-ToF-MS; O-glycosylation; T-cell receptor-γδ; intestinal intraepithelial T lymphocytes; mouse intestine