title: Genome sequence of the acid-tolerant Burkholderia sp. strain WSM2230 from Karijini National Park, Australia.
abstract: Burkholderia sp. strain WSM2230 is an aerobic, motile, Gram-negative, non-spore-forming acid-tolerant rod isolated from acidic soil collected in 2001 from Karijini National Park, Western Australia, using Kennedia coccinea (Coral Vine) as a host. WSM2230 was initially effective in nitrogen-fixation with K. coccinea, but subsequently lost symbiotic competence. Here we describe the features of Burkholderia sp. strain WSM2230, together with genome sequence information and its annotation. The 6,309,801 bp high-quality-draft genome is arranged into 33 scaffolds of 33 contigs containing 5,590 protein-coding genes and 63 RNA-only encoding genes. The genome sequence of WSM2230 failed to identify nodulation genes and provides an explanation for the observed failure of the laboratory grown strain to nodulate. The genome of this strain is one of 100 sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.
authors.affiliation: School of Biomedical Sciences, Faculty of Health Sciences, Curtin University, Western Australia, Australia.
authors.forename: Robert
authors.initials: R
authors.lastname: Walker
authors.affiliation: School of Biomedical Sciences, Faculty of Health Sciences, Curtin University, Western Australia, Australia.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Watkin
authors.affiliation: Centre for Rhizobium Studies, School of Veterinary and Life Sciences, Murdoch University, Western Australia, Australia.
authors.forename: Rui
authors.initials: R
authors.lastname: Tian
authors.affiliation: School of Life and Environmental Sciences, Deakin University, Victoria, Australia.
authors.forename: Lambert
authors.initials: L
authors.lastname: Bräu
authors.affiliation: Centre for Rhizobium Studies, School of Veterinary and Life Sciences, Murdoch University, Western Australia, Australia.
authors.forename: Graham
authors.initials: G
authors.lastname: O'Hara
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors.forename: Lynne
authors.initials: L
authors.lastname: Goodwin
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: James
authors.initials: J
authors.lastname: Han
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Lobos
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Marcel
authors.initials: M
authors.lastname: Huntemann
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Amrita
authors.initials: A
authors.lastname: Pati
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Tanja
authors.initials: T
authors.lastname: Woyke
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Mavromatis
authors.affiliation: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Victor
authors.initials: V
authors.lastname: Markowitz
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Ivanova
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Nikos
authors.initials: N
authors.lastname: Kyrpides
authors.affiliation: Centre for Rhizobium Studies, School of Veterinary and Life Sciences, Murdoch University, Western Australia, Australia.
authors.forename: Wayne
authors.initials: W
authors.lastname: Reeve
keywords: Betaproteobacteria; nitrogen fixation; rhizobia; root-nodule bacteria