title: Development of human serum albumin conjugated with near-infrared dye for photoacoustic tumor imaging.
abstract: Photoacoustic (PA) imaging has emerged as a noninvasive diagnostic method which detects ultrasonic waves thermoelastically induced by optical absorbers irradiated with laser. For tumor diagnosis, PA contrast agent has been proposed to enhance the PA effect for detecting tumors sensitively. Here, we prepared a human serum albumin (HSA) conjugated with indocyanine green (ICG) as a PA contrast agent allowing enhanced permeability and retention effect for sensitive tumor imaging. The feasibility of PA imaging with HSA-ICG to detect allografted tumors was evaluated in tumor-bearing mice. In vivo fluorescence imaging and radiolabeled biodistribution study showed that the biodistribution dramatically changed as the number of ICG bound to HSA increased, and the maximum accumulation of ICG was achieved when around three ICG molecules were loaded on an HSA. In vivo PA imaging demonstrated a tumor-selective and dose-dependent increase of PA signal intensity in mice injected with HSA-ICG (R2 = 0.88, 387% increase for HSA-ICG, 104 nmol ICG). In conclusion, HSA-ICG clearly visualized the allografted tumors with high tumor-to-background ratios having high quantitative and spatial resolution for the sensitive PA imaging of tumors. HSA-ICG could be useful as a favorable contrast agent for PA tumor imaging for the management of cancer.
authors.affiliation: Canon Inc., Medical Imaging Project, Corporate R&D Headquarters, 3-30-2 Shimomaruko, Ohta-ku, Tokyo 146-8501, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Kanazaki
authors.affiliation: Kyoto University, Department of Patho-Functional Bioanalysis Graduate School of Pharmaceutical Sciences, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, JapancKyoto University Hospital, 54 Kawaharacho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Sano
authors.affiliation: Kyoto University, Department of Patho-Functional Bioanalysis Graduate School of Pharmaceutical Sciences, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, JapandUniversity of Fukui, Biomedical Imaging Research Center, 23-3 Matsuokashimoaizuki, Eihe.
authors.forename: Akira
authors.initials: A
authors.lastname: Makino
authors.affiliation: Canon Inc., Medical Imaging Project, Corporate R&D Headquarters, 3-30-2 Shimomaruko, Ohta-ku, Tokyo 146-8501, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Takahashi
authors.affiliation: Kyoto University, Department of Patho-Functional Bioanalysis Graduate School of Pharmaceutical Sciences, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Deguchi
authors.affiliation: Kyoto University, Department of Patho-Functional Bioanalysis Graduate School of Pharmaceutical Sciences, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Manami
authors.initials: M
authors.lastname: Ohashi
authors.affiliation: Kyoto University, Department of Patho-Functional Bioanalysis Graduate School of Pharmaceutical Sciences, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Temma
authors.affiliation: Kyoto University, Department of Patho-Functional Bioanalysis Graduate School of Pharmaceutical Sciences, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ono
authors.affiliation: Kyoto University, Department of Patho-Functional Bioanalysis Graduate School of Pharmaceutical Sciences, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Saji
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D007205:Indium Radioisotopes / Q000737:chemistry / Q000493:pharmacokinetics; D007208:Indocyanine Green / Q000737:chemistry* / Q000493:pharmacokinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000175:diagnosis / Q000473:pathology*; D061848:Optical Imaging / Q000379:methods*; D004369:Pentetic Acid / Q000737:chemistry / Q000493:pharmacokinetics; D061088:Photoacoustic Techniques / Q000379:methods*; D012709:Serum Albumin / Q000737:chemistry* / Q000493:pharmacokinetics; D014018:Tissue Distribution
chemical_list: D007205:Indium Radioisotopes; D012709:Serum Albumin; D004369:Pentetic Acid; D007208:Indocyanine Green