title: Development of a novel liposomal nanodelivery system for bioluminescence imaging and targeted drug delivery in ErbB2-overexpressing metastatic ovarian carcinoma.
abstract: Liposomes as targeted drug delivery systems are an emerging strategy in the treatment of cancer to selectively target tumors or genes. In this study, we generated the recombinant protein, EC1-GLuc, by fusing the EC1 peptide, an artificial ligand of ErbB2, with Gaussia luciferase (GLuc). The purified EC1-GLuc was conjugated with a nickel-chelating liposome to construct the EC1-GLuc-liposome. In vitro experiments revealed that the EC1-GLuc-liposome selectively targeted and internalized into ErbB2-overexpressing SKOv3 cells for bioluminescence imaging. A cell-impermeable fluorescence dye (HPTS) encapsulated in the EC-GLuc-liposome was efficiently delivered into the SKOv3 cells. In addition, the EC1-GLuc-liposome also targeted metastatic SKOv3 tumors for bioluminescence imaging and effectively delivered HPTS into metastatic tumors in vivo. Therefore, the present study demonstrates the novel EC1-GLuc-liposome to be an effective theranostic system for monitoring and treating ErbB2-overexpressing metastatic ovarian carcinoma through a combination of targeted molecular imaging and DDS.
authors.affiliation: Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi, P.R. China.
authors.forename: Xiao-Jian
authors.initials: XJ
authors.lastname: Han
authors.affiliation: Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi, P.R. China.
authors.forename: Yong-Fang
authors.initials: YF
authors.lastname: Wei
authors.affiliation: Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi, P.R. China.
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Wan
authors.affiliation: Department of Pharmacology, Nanchang University School of Pharmaceutical Science, Nanchang, Jiangxi, P.R. China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Jiang
authors.affiliation: Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi, P.R. China.
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Zhang
authors.affiliation: Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi, P.R. China.
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Xin
mesh_terms: D000818:Animals; D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D006801:Humans; D008024:Ligands; D008081:Liposomes / Q000737:chemistry*; D008156:Luciferases / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D061986:MCF-7 Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics* / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D023041:Xenograft Model Antitumor Assays
chemical_list: D008024:Ligands; D008081:Liposomes; D008156:Luciferases; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human; C524056:Erbb2 protein, mouse