title: In vitro activities of amphotericin B deoxycholate and liposomal amphotericin B against 604 clinical yeast isolates.
abstract: We determined the in vitro antifungal activity of liposomal amphotericin B (L-AmB) against 604 clinical yeast isolates. Amphotericin B deoxycholate (D-AmB) was tested in parallel against all the isolates. Susceptibility testing was performed according to the Clinical and Laboratory Standards Institute (CLSI) M27-A3 method. Overall, L-AmB was highly active against the isolates (mean MIC, 0.42 µg ml(-1); MIC90, 1 µg ml(-1); 97.2 % of MICs were ≤1 µg ml(-1)) and comparable to D-AmB (mean MIC, 0.48 µg ml(-1); MIC90, 1 µg ml(-1); 97.3 % of MICs were ≤1 µg ml(-1)). The in vitro activity of D-AmB and L-AmB was correlated (R(2) = 0.61; exp(b), 2.3; 95 % CI, 2.19-2.44, P<0.001). Candida albicans (mean MICs of D-AmB and L-AmB, 0.39 µg ml(-1) and 0.31 µg ml(-1), respectively) and Candida parapsilosis (mean MICs of D-AmB and L-AmB, 0.38 µg ml(-1) and 0.35 µg ml(-1), respectively) were the species most susceptible to the agents tested, while Candida krusei (currently named Issatchenkia orientalis) (mean MICs of D-AmB and L-AmB, 1.27 µg ml(-1) and 1.13 µg ml(-1), respectively) was the least susceptible. The excellent in vitro activity of L-AmB may have important implications for empirical treatment approaches and support its role in treatment of a wide range of invasive infections due to yeasts.
authors.affiliation: Department of Biomedical Science and Human Oncology, Hygiene Section, University of Bari, Bari, Italy.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Montagna
authors.affiliation: Department of Biomedical Science and Human Oncology, Hygiene Section, University of Bari, Bari, Italy.
authors.forename: Grazia
authors.initials: G
authors.lastname: Lovero
authors.affiliation: Department of Biomedical Science and Human Oncology, Hygiene Section, University of Bari, Bari, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Coretti
authors.affiliation: Department of Biomedical Science and Human Oncology, Hygiene Section, University of Bari, Bari, Italy.
authors.forename: Osvalda
authors.initials: O
authors.lastname: De Giglio
authors.affiliation: Department of Medical and Surgical Sciences, Hygiene Section, University of Foggia, Foggia, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Martinelli
authors.affiliation: Department of Internal Medicine and Medical Specialties, Infectious Diseases Clinic, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Bedini
authors.affiliation: Department of Emergency and Organ Transplantation, Hematology Section, University of Bari, Bari, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Delia
authors.affiliation: Department of Pharmaceutical Chemistry, Section of Microbiology, Faculty of Pharmacy, University of Bari, Bari, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Rosato
authors.affiliation: Department of Internal Medicine and Medical Specialties, Infectious Diseases Clinic, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Codeluppi
authors.affiliation: Department of Biomedical Science and Human Oncology, Hygiene Section, University of Bari, Bari, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Caggiano
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans; D003840:Deoxycholic Acid / Q000494:pharmacology*; D004338:Drug Combinations; D006801:Humans; D008826:Microbial Sensitivity Tests; D009181:Mycoses / Q000382:microbiology*; D010843:Pichia; D015003:Yeasts / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D000935:Antifungal Agents; D004338:Drug Combinations; C068538:liposomal amphotericin B; D003840:Deoxycholic Acid; D000666:Amphotericin B; C059765:amphotericin B, deoxycholate drug combination