title: Microsatellite primers identified by 454 sequencing in the floodplain tree species Eucalyptus victrix (Myrtaceae).
abstract: OBJECTIVE
  Microsatellite primers were developed for Eucalyptus victrix (Myrtaceae) to evaluate the population and spatial genetic structure of this widespread northwestern Australian riparian tree species, which may be impacted by hydrological changes associated with mining activity. •
  
  
  RESULTS
  454 GS-FLX shotgun sequencing was used to obtain 1895 sequences containing putative microsatellite motifs. Ten polymorphic microsatellite loci were identified and screened for variation in individuals from two populations in the Pilbara region. Observed heterozygosities ranged from 0.44 to 0.91 (mean: 0.66) and the number of alleles per locus ranged from five to 25 (average: 11). •
  
  
  CONCLUSIONS
  These microsatellite loci will be useful in future studies of population and spatial genetic structure in E. victrix, and inform the development of seed sourcing strategies for the species.
authors.affiliation: Botanic Gardens and Parks Authority, Kings Park and Botanic Garden, West Perth 6005 Western Australia, Australia ; School of Plant Biology, University of Western Australia, Nedlands 6009 Western Australia, Australia.
authors.forename: Paul G
authors.initials: PG
authors.lastname: Nevill
authors.affiliation: Botanic Gardens and Parks Authority, Kings Park and Botanic Garden, West Perth 6005 Western Australia, Australia ; School of Plant Biology, University of Western Australia, Nedlands 6009 Western Australia, Australia.
authors.forename: Donna
authors.initials: D
authors.lastname: Bradbury
authors.affiliation: Natural History Museum of Denmark, University of Copenhagen, Copenhagen DK-1350, Denmark.
authors.forename: Tina
authors.initials: T
authors.lastname: Jørgensen
authors.affiliation: Botanic Gardens and Parks Authority, Kings Park and Botanic Garden, West Perth 6005 Western Australia, Australia ; School of Plant Biology, University of Western Australia, Nedlands 6009 Western Australia, Australia.
authors.forename: Siegfried
authors.initials: S
authors.lastname: Krauss
authors.affiliation: Rio Tinto Iron Ore, Level 22, 152-158 St. Georges Terrace, Perth 6000 Western Australia, Australia.
authors.forename: Sunil
authors.initials: S
authors.lastname: Samaraweera
authors.affiliation: School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide 5001 South Australia, Australia ; Australian Centre for Evolutionary Biology and Biodiversity, University of Adelaide, Adelaide 5005 South Australia, Australia.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Gardner
keywords: 454 GS-FLX; Eucalyptus victrix; microsatellite primers; northwestern Australia; riparian; shotgun sequencing