title: A draft genome of Escherichia coli sequence type 127 strain 2009-46.
abstract: BACKGROUND
  Escherichia coli are a frequent cause of urinary tract infections (UTI) and are thought to have a foodborne origin. E. coli with sequence type 127 (ST127) are emerging pathogens increasingly implicated as a cause of urinary tract infections (UTI) globally. A ST127 isolate (2009-46) resistant to ampicillin and trimethoprim was recovered from the urine of a 56 year old patient with a UTI from a hospital in Sydney, Australia and was characterised here.
  
  
  RESULTS
  We sequenced the genome of Escherichia coli 2009-46 using the Illumina Nextera XT and MiSeq technologies. Assembly of the sequence data reconstructed a 5.14 Mbp genome in 89 scaffolds with an N50 of 161 kbp. The genome has extensive similarity to other sequenced uropathogenic E. coli genomes, but also has several genes that are potentially related to virulence and pathogenicity that are not present in the reference E. coli strain.
  
  
  CONCLUSIONS
  E. coli 2009-46 is a multiple antibiotic resistant, phylogroup B2 isolate recovered from a patient with a UTI. This is the first description of a drug resistant E. coli ST127 in Australia.
authors.affiliation: ithree Institute, University of Technology Sydney, Broadway Street, 2007 Ultimo, Australia.
authors.forename: Aaron E
authors.initials: AE
authors.lastname: Darling
authors.affiliation: ithree Institute, University of Technology Sydney, Broadway Street, 2007 Ultimo, Australia.
authors.forename: Jessica
authors.initials: J
authors.lastname: McKinnon
authors.affiliation: ithree Institute, University of Technology Sydney, Broadway Street, 2007 Ultimo, Australia.
authors.forename: Paul
authors.initials: P
authors.lastname: Worden
authors.affiliation: ithree Institute, University of Technology Sydney, Broadway Street, 2007 Ultimo, Australia.
authors.forename: Jerran
authors.initials: J
authors.lastname: Santos
authors.affiliation: ithree Institute, University of Technology Sydney, Broadway Street, 2007 Ultimo, Australia.
authors.forename: Ian G
authors.initials: IG
authors.lastname: Charles
authors.affiliation: ithree Institute, University of Technology Sydney, Broadway Street, 2007 Ultimo, Australia.|NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Woodbridge Road, 2568 Menangle, Australia.
authors.forename: Piklu Roy
authors.initials: PR
authors.lastname: Chowdhury
authors.affiliation: ithree Institute, University of Technology Sydney, Broadway Street, 2007 Ultimo, Australia.
authors.forename: Steven P
authors.initials: SP
authors.lastname: Djordjevic
keywords: Escherichia coli 2009-46; Genome; Sequencing