title: Study of leaf metabolome modifications induced by UV-C radiations in representative Vitis, Cissus and Cannabis species by LC-MS based metabolomics and antioxidant assays.
abstract: UV-C radiation is known to induce metabolic modifications in plants, particularly to secondary metabolite biosynthesis. To assess these modifications from a global and untargeted perspective, the effects of the UV-C radiation of the leaves of three different model plant species, Cissus antarctica Vent. (Vitaceae), Vitis vinifera L. (Vitaceae) and Cannabis sativa L. (Cannabaceae), were evaluated by an LC-HRMS-based metabolomic approach. The approach enabled the detection of significant metabolite modifications in the three species studied. For all species, clear modifications of phenylpropanoid metabolism were detected that led to an increased level of stilbene derivatives. Interestingly, resveratrol and piceid levels were strongly induced by the UV-C treatment of C. antarctica leaves. In contrast, both flavonoids and stilbene polymers were upregulated in UV-C-treated Vitis leaves. In Cannabis, important changes in cinnamic acid amides and stilbene-related compounds were also detected. Overall, our results highlighted phytoalexin induction upon UV-C radiation. To evaluate whether UV-C stress radiation could enhance the biosynthesis of bioactive compounds, the antioxidant activity of extracts from control and UV-C-treated leaves was measured. The results showed increased antioxidant activity in UV-C-treated V. vinifera extracts.
authors.affiliation: School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, Quai Ernest-Ansermet 30, Geneva CH-1211, Switzerland. guillaume.marti@univ-tlse3.fr.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Marti
authors.affiliation: Station de recherche Agroscope, Institut des Sciences en Production Végétale IPV, Route de Duiller 50, P.O. Box 1012, Nyon 1260, Switzerland. sylvain.schnee@agroscope.admin.ch.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Schnee
authors.affiliation: School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, Quai Ernest-Ansermet 30, Geneva CH-1211, Switzerland. yannis.andrey@gmail.com.
authors.forename: Yannis
authors.initials: Y
authors.lastname: Andrey
authors.affiliation: School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, Quai Ernest-Ansermet 30, Geneva CH-1211, Switzerland. claudia.avello@unige.ch.
authors.forename: Claudia
authors.initials: C
authors.lastname: Simoes-Pires
authors.affiliation: School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, Quai Ernest-Ansermet 30, Geneva CH-1211, Switzerland. pierre-alain.carrupt@unige.ch.
authors.forename: Pierre-Alain
authors.initials: PA
authors.lastname: Carrupt
authors.affiliation: School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, Quai Ernest-Ansermet 30, Geneva CH-1211, Switzerland. jean-luc.wolfender@unige.ch.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Wolfender
authors.affiliation: Station de recherche Agroscope, Institut des Sciences en Production Végétale IPV, Route de Duiller 50, P.O. Box 1012, Nyon 1260, Switzerland. katia.gindro@agroscope.admin.ch.
authors.forename: Katia
authors.initials: K
authors.lastname: Gindro
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002188:Cannabis / Q000378:metabolism* / Q000528:radiation effects; D032422:Cissus / Q000378:metabolism* / Q000528:radiation effects; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification; D005609:Free Radicals / Q000737:chemistry; D055442:Metabolome / Q000528:radiation effects; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D018515:Plant Leaves / Q000378:metabolism* / Q000528:radiation effects; D021241:Spectrometry, Mass, Electrospray Ionization; D013451:Sulfonic Acids / Q000737:chemistry; D014466:Ultraviolet Rays; D027843:Vitis / Q000378:metabolism* / Q000528:radiation effects
chemical_list: D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl