title: Transcription efficiency of different chicken mannose-binding lectin promoter alleles.
abstract: The serum collectin mannose-binding lectin (MBL) plays a major role in innate immunity by activation of the lectin complement pathway or by acting as an opsonin. The serum levels of human and animal MBL are associated with susceptibility to a wide range of infections, and the variation of MBL in serum is genetically determined. In the chicken, 14 single nucleotide polymorphisms (SNPs) have so far been found in the MBL promoter region. In this study, the transcription activity of a 670-bp promoter region covering all 14 SNPs from the four MBL promoter alleles A1 to A4 was assessed using a dual-luciferase assay. Of the analysed alleles, A1 showed the highest transcription activity although this allele is frequently found in chickens with low MBL mRNA expression.
authors.affiliation: Department of Animal Science, Aarhus University, Blichers Allé 20, P.O. Box 50, 8830, Tjele, Denmark.
authors.forename: R M
authors.initials: RM
authors.lastname: Kjærup
authors.forename: T S
authors.initials: TS
authors.lastname: Dalgaard
authors.forename: L R
authors.initials: LR
authors.lastname: Norup
authors.forename: R M
authors.initials: RM
authors.lastname: Goto
authors.forename: M M
authors.initials: MM
authors.lastname: Miller
authors.forename: P
authors.initials: P
authors.lastname: Sørensen
authors.forename: H R
authors.initials: HR
authors.lastname: Juul-Madsen
mesh_terms: D000483:Alleles; D000818:Animals; D002645:Chickens / Q000235:genetics*; D015870:Gene Expression; D023061:Gene Order; D017930:Genes, Reporter; D037601:Mannose-Binding Lectin / Q000097:blood / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D037601:Mannose-Binding Lectin