title: [Serum glycoprotein profiling by lectin affinity microarray to distinguish the various stages of primary liver carcinogenesis].
abstract: OBJECTIVE
  To identify specific serum glycoprotein profiles that correspond to the carcinogenic process of primary liver cancer (PLC) by analyzing a population with high-incidence of PLC using lectin affinity microarray.
  
  
  METHODS
  Serum samples were collected from individuals classified as high risk for PLC (including patients with liver cirrhosis and hepatitis B) and development of PLC was recorded. Healthy individuals served as normal controls. The serum samples were subjected to glycoprotein profling by using lectin microarrays and the results were confirmed by lectin blot. Between-group differences were statistically analyzed.
  
  
  RESULTS
  PLC carcinogenesis was found to be correlated with enhanced affinity for AAL, ACL, ConA, LCA, MPL, NML, PHA-E, PHA-L, PSA, RCA-I, STL, VAL,WGA, and SNA (P less than 0.05). These data implied that changes in specific glycan structures, such as aFuc, GlcNAc, GalNAc, mannose, bisecting GlcNAc and terminal beta1-4 Gal, may be involved in PLC carcinogenesis . The PLC group showed significantly different results for all detected lectins, except SNA (P less than 0.05). However, among the PLC group, the SNA affinity was not significantly different for the hepatitis B group (P =0.443, P more than 0.05).
  
  
  CONCLUSIONS
  Glycans may be associated with the carcinogenic process of PLC and may be developed as diagnostic and prognostic biomarkers of PLC in the future.
authors.affiliation: The Affiliatde Tumor Hospital of Guangxi Medical university, Nanning 530021, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Jing
authors.forename: Heng
authors.initials: H
authors.lastname: Hu
authors.forename: Chun
authors.initials: C
authors.lastname: Sun
authors.forename: Tianren
authors.initials: T
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Deng
authors.forename: Jilin
authors.initials: J
authors.lastname: Li
authors.forename: Jiahua
authors.initials: J
authors.lastname: Yu
authors.forename: Yinkun
authors.initials: Y
authors.lastname: Liu
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhang
mesh_terms: D063646:Carcinogenesis; D002846:Chromatography, Affinity; D015331:Cohort Studies; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D037102:Lectins / Q000097:blood*; D008113:Liver Neoplasms / Q000097:blood* / Q000473:pathology*
chemical_list: D006023:Glycoproteins; D037102:Lectins