title: The activity-integrated method for quality assessment of reduning injection by on-line DPPH-CE-DAD.
abstract: A sensitive on-line DPPH-CE-DAD method was developed and validated for both screening and determining the concentration of seven antioxidants of Reduning injection. The pH and concentrations of buffer solution, SDS, β-CD and organic modifier were studied for the detection of DPPH and seven antioxidants. By on-line mixing DPPH and sample solution, a DPPH-CE method for testing the antioxidant activity of the complex matrix was successfully established and used to screen the antioxidant components of Reduning injection. Then, antioxidant components including caffeic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, chlorogenic acid, neochlorogenic acid and cryptochlorogenic acid were quantified by the newly established CE-DAD method. Finally, the total antioxidant activity and the multiple active components were selected as markers to evaluate the quality of Reduning injection. The results demonstrated that the on-line DPPH-CE-DAD method was reagent-saving, rapid and feasible for on-line simultaneous determination of total pharmacological activity and contents of multi-components samples. It was also a powerful method for evaluating the quality control and mechanism of action of TCM injection.
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China; State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Kanion Pharmaceutical Co., Ltd, Lianyungang, China.
authors.forename: Yan-xu
authors.initials: YX
authors.lastname: Chang
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Liu
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
authors.forename: Er-wei
authors.initials: EW
authors.lastname: Liu
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
authors.forename: Xiu-cheng
authors.initials: XC
authors.lastname: Jiao
authors.affiliation: State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Kanion Pharmaceutical Co., Ltd, Lianyungang, China.
authors.forename: Zhen-zhong
authors.initials: ZZ
authors.lastname: Wang
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Gao
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
authors.forename: Bo-li
authors.initials: BL
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Kanion Pharmaceutical Co., Ltd, Lianyungang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000975:Antioxidants / Q000032:analysis; D001713:Biphenyl Compounds / Q000737:chemistry*; D002138:Calibration; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D004560:Electricity; D004581:Electronics / Q000295:instrumentation*; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D020128:Inhibitory Concentration 50; D007267:Injections; D057230:Limit of Detection; D009862:Online Systems; D010851:Picrates / Q000737:chemistry*; D012015:Reference Standards; D013696:Temperature; D013997:Time Factors; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D000975:Antioxidants; D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D010851:Picrates; D047392:beta-Cyclodextrins; C000596355:reduning; C004931:1,1-diphenyl-2-picrylhydrazyl; C031215:betadex; C032159:acetonitrile