title: Protective effect of kaempferol on LPS plus ATP-induced inflammatory response in cardiac fibroblasts.
abstract: Inflammatory response is an important mechanism in the pathogenesis of cardiovascular diseases. Cardiac fibroblasts play a crucial role in cardiac inflammation and might become a potential therapeutic target in cardiovascular diseases. Kaempferol, a flavonoid commonly existing in many edible fruits, vegetables, and Chinese herbs, is well known to possess anti-inflammatory property and thus has a therapeutic potential for the treatment of inflammatory diseases. To date, the effect of kaempferol on cardiac fibroblasts inflammation is unknown. In this study, we investigated the anti-inflammatory effect of kaempferol on lipopolysaccharide (LPS) plus ATP-induced cardiac fibroblasts and explored the underlying mechanisms. Our results showed that kaempferol at concentrations of 12.5 and 25 μg/mL significantly suppressed the release of TNF-α, IL-1β, IL-6, and IL-18 and inhibited activation of NF-κB and Akt in LPS plus ATP-induced cardiac fibroblasts. These findings suggest that kaempferol attenuates cardiac fibroblast inflammation through suppression of activation of NF-κB and Akt.
authors.affiliation: Experimental Research Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China.
authors.forename: Xi-Lan
authors.initials: XL
authors.lastname: Tang
authors.forename: Jian-Xun
authors.initials: JX
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Dong
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Jin-Cai
authors.initials: JC
authors.lastname: Hou
authors.forename: Yong-Qiu
authors.initials: YQ
authors.lastname: Zheng
authors.forename: Cheng-Ren
authors.initials: CR
authors.lastname: Lin
authors.forename: Jun-Guo
authors.initials: JG
authors.lastname: Ren
mesh_terms: D000255:Adenosine Triphosphate / Q000633:toxicity*; D000818:Animals; D000831:Animals, Newborn; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism / Q000517:prevention & control; D044949:Kaempferols / Q000494:pharmacology* / Q000627:therapeutic use; D008070:Lipopolysaccharides / Q000633:toxicity*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D017207:Rats, Sprague-Dawley
chemical_list: D000893:Anti-Inflammatory Agents; D044949:Kaempferols; D008070:Lipopolysaccharides; C006552:kaempferol; D000255:Adenosine Triphosphate