title: Cytokinin producing bacteria stimulate amino acid deposition by wheat roots.
abstract: Phytohormone production is one mechanism by which rhizobacteria can stimulate plant growth, but it is not clear whether the bacteria gain from this mechanism. The hypothesis that microbial-derived cytokinin phytohormones stimulate root exudation of amino acids was tested. The rhizosphere of wheat plants was drenched with the synthetic cytokinin trans-zeatin or inoculated with Bacillus subtilis IB-22 (which produces zeatin type cytokinins) or B. subtilis IB-21 (which failed to accumulate cytokinins). Growing plants in a split root system allowed spatial separation of zeatin application or rhizobacterial inoculation to one compartment and analyses of amino acid release from roots (rhizodeposition) into the other compartment (without either microbial inoculation or treatment with exogenous hormone). Supplying B. subtilis IB-22 or zeatin to either the whole root system or half of the roots increased concentrations of amino acids in the soil solution although the magnitude of the increase was greater when whole roots were treated. There was some similarity in amino acid concentrations induced by either bacterial or zeatin treatment. Thus B. subtilis IB-22 increased amino acid rhizodeposition, likely due to its ability to produce cytokinins. Furthermore, B. subtilis strain IB-21, which failed to accumulate cytokinins in culture media, did not significantly affect amino acid concentrations in the wheat rhizosphere. The ability of rhizobacteria to produce cytokinins and thereby stimulate rhizodeposition may be important in enhancing rhizobacterial colonization of the rhizoplane.
authors.affiliation: Institute of Biology, Ufa Science Centre, Russian Academy of Sciences, pr. Oktyabrya, 69, 450054 Ufa, Russia. Electronic address: guzel@anrb.ru.
authors.forename: Guzel R
authors.initials: GR
authors.lastname: Kudoyarova
authors.affiliation: Institute of Biology, Ufa Science Centre, Russian Academy of Sciences, pr. Oktyabrya, 69, 450054 Ufa, Russia.
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Melentiev
authors.affiliation: Institute of Biology, Ufa Science Centre, Russian Academy of Sciences, pr. Oktyabrya, 69, 450054 Ufa, Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Martynenko
authors.affiliation: Institute of Biology, Ufa Science Centre, Russian Academy of Sciences, pr. Oktyabrya, 69, 450054 Ufa, Russia.
authors.forename: Leila N
authors.initials: LN
authors.lastname: Timergalina
authors.affiliation: Institute of Biology, Ufa Science Centre, Russian Academy of Sciences, pr. Oktyabrya, 69, 450054 Ufa, Russia.
authors.forename: Tatiana N
authors.initials: TN
authors.lastname: Arkhipova
authors.affiliation: Institute of Biology, Ufa Science Centre, Russian Academy of Sciences, pr. Oktyabrya, 69, 450054 Ufa, Russia.
authors.forename: Galina V
authors.initials: GV
authors.lastname: Shendel
authors.affiliation: Institute of Biology, Ufa Science Centre, Russian Academy of Sciences, pr. Oktyabrya, 69, 450054 Ufa, Russia.
authors.forename: Ludmila Yu
authors.initials: LY
authors.lastname: Kuz'mina
authors.affiliation: The Lancaster Environment Centre, Lancaster University, LA1 4YQ Lancaster, UK.
authors.forename: Ian C
authors.initials: IC
authors.lastname: Dodd
authors.affiliation: Bashkir State University, Zaki-Validi Street, 32, 450074 Ufa, Russia.
authors.forename: Stanislav Yu
authors.initials: SY
authors.lastname: Veselov
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D001412:Bacillus subtilis / Q000378:metabolism*; D003583:Cytokinins / Q000096:biosynthesis*; D027343:Rhizome / Q000378:metabolism / Q000382:microbiology; D014908:Triticum / Q000378:metabolism / Q000382:microbiology
chemical_list: D000596:Amino Acids; D003583:Cytokinins
keywords: Bacillus subtilis; Cytokinin; Plant/microbe interaction; Rhizodeposition; Wheat