title: Polymorphism in miRNA-1 target site and circulating miRNA-1 phenotype are associated with the decreased risk and prognosis of coronary artery disease.
abstract: MiRNA molecules have been identified to play key roles in a broad range of physiologic and pathologic processes. Polymorphisms in microRNA target sites (PolymiRTSs) can disturb or obstruct miRNA binding and consequentially influence regulation of the target genes. A two-step study design was used in this study. A case-control study was designed to assess the relationship between miRNA-1 target site rs9548934C→T polymorphism in target gene (Component of Oligomeric Golgi Complex 6, COG6) and risk of coronary artery disease (CAD) in 1013 patients and 610 normal controls. This genetic variant was also evaluated for the association with major adverse cardiovascular events (MACE) of CAD in a follow-up study, including 785 (785/1013) patients followed up for 42 months. The phenotypes of circulating miRNA-1 levels in 34 cases were slightly lower than that of 40 controls but not significantly different (P = 0.090). The CT and CT/TT genotypes were associated with a 34% and 26% decreased risk of CAD, and the TT and CT/TT genotypes were associated with a 76% and 49% decreased risk of MACE of CAD. Cox regression analysis showed that rs9548937 C/T variant was associated with a decreased risk of MACE, while age, diabetes mellitus, higher levels of CRP (≥ 3.80 mg/L) and three pathological changes in the coronary artery were associated with an increased risk of MACE. Our findings implicate miRNA-1 target site rs9548934C→T genotypes, circulating miRNA-1 phenotype and CRP levels may modulate the occurrence and MACE of CAD.
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, Department of Epidemiology & Biostatistics, School of Public Health, Southeast University Nanjing, China ; School of Biological Science and Medical Engineering, Southeast University Nanjing, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Zhongda Hospital, Southeast University Nanjing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhi
authors.affiliation: Department of Cardiology, Zhongda Hospital, Southeast University Nanjing, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Cardiology, Zhongda Hospital, Southeast University Nanjing, China.
authors.forename: Genshan
authors.initials: G
authors.lastname: Ma
authors.affiliation: Department of Cardiology, Zhongda Hospital, Southeast University Nanjing, China.
authors.forename: Xingzhou
authors.initials: X
authors.lastname: Ye
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, Department of Epidemiology & Biostatistics, School of Public Health, Southeast University Nanjing, China.
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Yu
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, Department of Epidemiology & Biostatistics, School of Public Health, Southeast University Nanjing, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of Cardiology, Zhongda Hospital, Southeast University Nanjing, China.
authors.forename: Han
authors.initials: H
authors.lastname: Jin
authors.affiliation: School of Biological Science and Medical Engineering, Southeast University Nanjing, China.
authors.forename: Zuhong
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, Department of Epidemiology & Biostatistics, School of Public Health, Southeast University Nanjing, China.
authors.forename: Pingmin
authors.initials: P
authors.lastname: Wei
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics*; D000368:Aged; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood* / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; C553748:COG6 protein, human; C544547:MIRN1 microRNA, human; D035683:MicroRNAs
keywords: MACE; coronary artery disease; miRNAs; polymorphism; target gene