title: Performance of four commercial kits for laboratory diagnosis of urogenital mollicute infection.
abstract: We have empirical evidence for the unsatisfactory performance of the most accessible commercial kits for mollicute culture. We aimed to evaluate the performance of the 4 commercial kits available for diagnosis of urogenital mollicute infections, compared with culture media (CMMC) produced in our research laboratory. We demonstrated that the NewProv kit had a sensitivity of 17% for Ureaplasma sp. and 33% for Mycoplasma hominis. The Laborclin kit presented a sensitivity of 25% for Ureaplasma sp., although M. hominis isolation was not observed during its evaluation. The kits from bioMérieux and International Microbio/Elitech each presented a sensitivity of 100% compared to the CMMC media. We also observed an important level of mollicute resistance (37.5%) to the main antibiotics used in treatment. The lack of diagnostic sensitivity of some commercial systems has consequences for antibiotic resistance, since it may lead to inadequate treatment of urogenital mollicute infections.
authors.affiliation: a Universidade Federal de Santa Catarina, Programa de Pós-graduação em Farmácia - Análises Clínicas.
authors.forename: Laís Del Prá Netto
authors.initials: Ldel P
authors.lastname: Machado
authors.forename: Marcelo A
authors.initials: MA
authors.lastname: Molinari
authors.forename: Larissa
authors.initials: L
authors.lastname: dos Santos
authors.forename: Caio M M
authors.initials: CM
authors.lastname: de Cordova
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003470:Culture Media; D004352:Drug Resistance, Microbial; D005260:Female; D052776:Female Urogenital Diseases / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D009175:Mycoplasma Infections / Q000175:diagnosis* / Q000382:microbiology; D019535:Mycoplasma hominis / Q000187:drug effects / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014509:Ureaplasma / Q000187:drug effects / Q000302:isolation & purification; D016869:Ureaplasma Infections / Q000175:diagnosis* / Q000382:microbiology; D014621:Vagina / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D011933:Reagent Kits, Diagnostic
keywords: Mycoplasma; Ureaplasma; culture; mollicute; resistance; résistance