title: Polymorphic microsatellite loci for Virola sebifera (Myristicaceae) derived from shotgun 454 pyrosequencing.
abstract: OBJECTIVE
  Polymorphic microsatellite loci were characterized in the dioecious neotropical rainforest tree Virola sebifera. The markers will be used to study ecological and genetic impacts of hunting and landscape change in this vertebrate-dispersed, insect-pollinated tree species.
  
  
  RESULTS
  Simple sequence repeat (SSR) markers were screened from genomic libraries of South American V. sebifera obtained by shotgun 454 pyrosequencing. Primer pairs were tested on Panamanian samples (N = 42). Approximately 52% of the 61 tested SSR markers amplified, and 16% were polymorphic. Ten selected polymorphic SSR loci contained seven to 15 alleles per locus, and polymorphic information content averaged 0.694. Observed heterozygosity ranged from 0.465 to 0.905, and expected heterozygosity was between 0.477 and 0.876.
  
  
  CONCLUSIONS
  The 10 polymorphic loci will be useful in studying gene flow and genetic structure at local and regional spatial scales in V. sebifera.
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Michigan, 830 North University Avenue, Ann Arbor, Michigan 48109-1048 USA.
authors.forename: Na
authors.initials: N
authors.lastname: Wei
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Michigan, 830 North University Avenue, Ann Arbor, Michigan 48109-1048 USA ; Smithsonian Tropical Research Institute, P.O. Box 0843-03092, Balboa, Ancón, Republic of Panama.
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Dick
authors.affiliation: Australian Centre for Evolutionary Biology and Biodiversity, School of Earth and Environmental Science and Environment Institute, University of Adelaide, North Terrace, Adelaide, South Australia 5001, Australia ; Science Resource Centre, Department of Environment and Natural Resources, Adelaide, South Australia 5005, Australia.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Lowe
authors.affiliation: Australian Centre for Evolutionary Biology and Biodiversity, School of Earth and Environmental Science and Environment Institute, University of Adelaide, North Terrace, Adelaide, South Australia 5001, Australia ; School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, South Australia 5001, Australia ; Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, South Australia 5001, Australia.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Gardner
keywords: Myristicaceae; Virola sebifera; microsatellite loci; shotgun 454 pyrosequencing