title: New microsatellite markers for Anthyllis vulneraria (Fabaceae), analyzed with Spreadex gel electrophoresis.
abstract: OBJECTIVE
  New microsatellite primers were developed for the diploid herb Anthyllis vulneraria. These primers will be used in upcoming studies focusing on random genetic variation, local adaptation, and phenotypic plasticity in alpine plants. •
  
  
  RESULTS
  The new primers were adjusted to separate PCR amplicons (70 to 170 bp) on precast Spreadex gels using horizontal gel electrophoresis. No capillary sequencer was needed. Three to twelve alleles were found per locus depending on the population studied. •
  
  
  CONCLUSIONS
  Our preliminary results showed that the three studied alpine populations are predominantly outcrossing, but include variable levels of self-fertilization.
authors.affiliation: Department of Environmental Sciences, Population Biology of Plants, University of Basel, Schönbeinstrasse 6, CH-4056 Basel, Switzerland.
authors.forename: Halil
authors.initials: H
authors.lastname: Kesselring
authors.affiliation: Department of Environmental Sciences, Population Biology of Plants, University of Basel, Schönbeinstrasse 6, CH-4056 Basel, Switzerland.
authors.forename: Elena
authors.initials: E
authors.lastname: Hamann
authors.affiliation: Department of Environmental Sciences, Population Biology of Plants, University of Basel, Schönbeinstrasse 6, CH-4056 Basel, Switzerland.
authors.forename: Jürg
authors.initials: J
authors.lastname: Stöcklin
authors.affiliation: Department of Environmental Sciences, Population Biology of Plants, University of Basel, Schönbeinstrasse 6, CH-4056 Basel, Switzerland.
authors.forename: Georg F J
authors.initials: GF
authors.lastname: Armbruster
keywords: Anthyllis vulneraria; Fabaceae; alpine plants; ethidium bromide; horizontal electrophoresis; microsatellites