title: An improved stool concentration procedure for the detection of Cryptosporidium oocysts in Orang Asli stool samples.
abstract: To improve the stool concentration procedure, we modified different steps of the standard formalin-ether concentration technique and evaluated these modifications by examining stool samples collected in the field. Seven samples were found positive by the modified formalin-ether concentration technique (M-FECT). Therefore, the M-FECT procedure provides enhanced detection of Cryptosporidium oocysts.
authors.affiliation: Department of Parasitology and Medical Entomology, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, 56000 Kuala Lumpur, Malaysia.
authors.forename: Fatmah Md
authors.initials: FM
authors.lastname: Salleh
authors.affiliation: Department of Parasitology and Medical Entomology, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, 56000 Kuala Lumpur, Malaysia.
authors.forename: Norhayati
authors.initials: N
authors.lastname: Moktar
authors.affiliation: Department of Parasitology and Medical Entomology, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, 56000 Kuala Lumpur, Malaysia.
authors.forename: Azlin Mohd
authors.initials: AM
authors.lastname: Yasin
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Hesham M
authors.initials: HM
authors.lastname: Al-Mekhlafi
authors.affiliation: Department of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA, Puncak Alam Campus, 42300 Selangor, Malaysia. Electronic address: tengku9235@puncakalam.uitm.edu.my.
authors.forename: Tengku Shahrul
authors.initials: TS
authors.lastname: Anuar
mesh_terms: D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000175:diagnosis* / Q000382:microbiology; D003458:Cryptosporidium / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D005260:Female; D006801:Humans; D008297:Male; D008828:Microbiological Techniques / Q000379:methods*; D008875:Middle Aged; D010274:Parasitology / Q000379:methods*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*
keywords: Concentration technique; Cryptosporidium oocysts; Formalin–ether