title: [Culture conditions optimization and high cell density fermentation of recombinant bacteria producing heparinase II from Flavobacterium heparinum].
abstract: Heparinase II (Hep II) from Flavobacterium heparinum is an enzyme that could specifically cleave certain sequence of heparin and heparan sulfate. In this work, fermentation conditions of recombinant heparinase II (His-Hep II) producing bacteria were optimized, including initial induction time, inducer (IPTG) concentration, induction temperature and induction time. The optimum conditions were as follows: cultivating recombinant bacteria to exponential prophase under 37 degrees C, then adding IPTG to a final concentration of 0.3 g/L, finally cultivating recombinant bacteria under 20 degrees C for 10 h. The total crude enzyme activity reached 570 U/L. Based on these results, high cell density fermentation of recombinant bacteria was studied. The final OD600 could reach 98 and the total crude enzyme activity of His-Hep II increased to 9 436 U/L.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Cheng
authors.forename: Chao
authors.initials: C
authors.lastname: Deng
authors.forename: Weichao
authors.initials: W
authors.lastname: Liu
authors.forename: Chaoliang
authors.initials: C
authors.lastname: Chen
authors.forename: Jinghua
authors.initials: J
authors.lastname: Chen
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Xu
mesh_terms: D005285:Fermentation; D005417:Flavobacterium / Q000378:metabolism*; D008828:Microbiological Techniques; D011133:Polysaccharide-Lyases / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: D011994:Recombinant Proteins; D011133:Polysaccharide-Lyases; C114663:heparinase II