title: Impact of antibacterial drugs on human serum paraoxonase-1 (hPON1) activity: an in vitro study.
abstract: OBJECTIVE
  To investigate the in vitro effects of the antibacterial drugs, meropenem trihydrate, piperacillin sodium, and cefoperazone sodium, on the activity of human serum paraoxonase (hPON1).
  
  
  METHODS
  hPON1 was purified from human serum using simple chromatographic methods, including DEAE-Sephadex anion exchange and Sephadex G-200 gel filtration chromatography.
  
  
  RESULTS
  The three antibacterial drugs decreased in vitro hPON1 activity. Inhibition mechanisms meropenem trihydrate was noncompetitive while piperacillin sodium and cefoperazone sodium were competitive.
  
  
  CONCLUSIONS
  Our results showed that antibacterial drugs significantly inhibit hPON1 activity, both in vitro, with rank order meropenem trihydrate piperacillin sodium cefoperazone sodium in vitro.
authors.affiliation: Department of Primary Education, Faculty of Education, Bayburt University, 69000 Bayburt, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Söyüt
authors.affiliation: Department of Food Engineering, Faculty of Engineering, Iğdır University, 76000 Iğdır, Turkey.
authors.forename: Elif Duygu
authors.initials: ED
authors.lastname: Kaya
authors.affiliation: Department of Chemistry, Faculty of Science, Atatürk University, 25240 Erzurum, Turkey.
authors.forename: Sükrü
authors.initials: S
authors.lastname: Beydemir
keywords: Cefoperazone sodium; Inhibition; Meropenem trihydrate; Paraoxonase; Piperacillin sodium