title: Relationship between oxidative stress and bone mass in obesity and effects of berry supplementation on bone remodeling in obese male mice: an exploratory study.
abstract: Berry consumption can prevent bone loss. However, the effects of different berries with distinct anthocyanin composition have not been thoroughly examined. The present study compared the effects of blueberry, blackberry, and black currant on bone health using a mouse model of diet-induced obesity. To investigate the effect of different berry supplements against a high-fat (HF) diet in vivo, 40 HF diet-induced obese (DIO) C57BL mice were assigned into four groups and fed a HF diet (35% w/w) with or without berry supplementation for 12 weeks (n=10). We measured adipose tissue mass (epididymal and retroperitoneal), plasma antioxidant, bone-related biomarkers, femur bone mineral density (BMD), and bone mineral content (proximal and distal). Adipose masses were negatively correlated with proximal BMD, but positively associated with plasma superoxide dismutase (SOD) concentrations (P<.001). Berry supplementation did not change the plasma ferric reducing antioxidant power, SOD, and insulin-like growth factor-1. However, the black currant group exhibited greater plasma alkaline phosphatase compared with the control group (P<.05). BMD in the distal epiphysis was significantly different between the blueberry and blackberry group (P<.05). However, berry supplementation did not affect bone mass compared with control. The present study demonstrates a negative relationship between fat mass and bone mass. In addition, our findings suggest that the anthocyanin composition of berries will affect bone turnover, warranting further research to investigate the underlying mechanisms.
authors.affiliation: 1 Department of Nutritional Sciences, University of Connecticut , Storrs, Connecticut, USA .
authors.forename: Sang Gil
authors.initials: SG
authors.lastname: Lee
authors.forename: Bohkyung
authors.initials: B
authors.lastname: Kim
authors.forename: Do Yu
authors.initials: do Y
authors.lastname: Soung
authors.forename: Terrence
authors.initials: T
authors.lastname: Vance
authors.forename: Jong Suk
authors.initials: JS
authors.lastname: Lee
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Sung I
authors.initials: SI
authors.lastname: Koo
authors.forename: Dae-Ok
authors.initials: DO
authors.lastname: Kim
authors.forename: Hicham
authors.initials: H
authors.lastname: Drissi
authors.forename: Ock K
authors.initials: OK
authors.lastname: Chun
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D000872:Anthocyanins / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification; D029798:Blueberry Plants / Q000737:chemistry*; D015519:Bone Density / Q000187:drug effects*; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism; D019587:Dietary Supplements / Q000032:analysis; D005638:Fruit / Q000737:chemistry; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D031965:Ribes / Q000737:chemistry*; D065856:Rubus / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D010936:Plant Extracts
keywords: anthocyanins; antioxidant; berries; bone turnover; high-fat diet