title: Detection of quinolone-resistance mutations of parC gene in clinical isolates of Acinetobacter baumannii in Iran.
abstract: BACKGROUND
  The purpose of this study was to screen of parC gene mutations in clinical isolates of Acinetobacter baumannii from intensive care units (ICUs) of Alzahra Hospital, Isfahan, Iran.
  
  
  METHODS
  Seventy isolates of A. baumannii between March 2011 and June 2012 were studied. Susceptibility test was established by E-test method. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing was performed for detection of parC gene mutation.
  
  
  RESULTS
  77.1% of specimens were highly resistant. Mutation at position 80 in parC was observed in 93% of isolates.
  
  
  CONCLUSIONS
  High proportion of A. baumannii isolates had a mutation in parC that can play an important role in increased incidence of these isolates.
authors.affiliation: Department of Microbiology, Science and Research Branch, Islami Azad University, Kerman, Iran.
authors.forename: Bahareh
authors.initials: B
authors.lastname: Vakili
authors.affiliation: Assistant Professor, Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Farzin
authors.initials: F
authors.lastname: Khorvash
authors.affiliation: Department of Microbiology and Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Fazeli
authors.affiliation: Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Moj
authors.initials: M
authors.lastname: Khaleghi
keywords: A. baumannii; multidrug resistant; parC gene