title: Blood pressure, heart rate and tubuloglomerular feedback in A1AR-deficient mice with different genetic backgrounds.
abstract: OBJECTIVE
  Differences in genetic background between control mice and mice with targeted gene mutations have been recognized as a potential cause for phenotypic differences. In this study, we have used A1AR-deficient mice in a C57Bl/6 and SWR/J congenic background to assess the influence of background on the effect of A1AR-deficiency on cardiovascular and renal functional parameters.
  
  
  METHODS
  In A1AR+/+ and A1AR-/- mice in C57Bl/6 and SWR/J congenic backgrounds, we assessed blood pressure and heart rate using radio-telemetry, plasma renin concentrations and tubuloglomerular feedback.
  
  
  RESULTS
  We did not detect significant differences in arterial blood pressure (MAP) and heart rates (HR) between A1AR+/+ and A1AR-/- mice in either C57Bl/6, SWR/J or mixed backgrounds. MAP and HR were significantly higher in SWR/J than in C57Bl/6 mice. A high NaCl intake increased MAP in A1AR-/- mice on C57Bl/6 background while there was less or no salt sensitivity in the SWR/J background. No significant differences in plasma renin concentration were detected between A1AR-/- and A1AR+/+ mice in any of the strains. Tubuloglomerular feedback was found to be absent in A1AR-/- mice with SWR/J genetic background.
  
  
  CONCLUSIONS
  While this study confirmed important differences between inbred mouse strains, we did not identify phenotypic modifications of A1AR-related effects on blood pressure, heart rate and plasma renin by differences in genetic background.
authors.affiliation: Department of Physiology, Chonbuk National University Medical School, Jeonju, South Korea; National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, Maryland.
authors.forename: S M
authors.initials: SM
authors.lastname: Kim
authors.forename: D
authors.initials: D
authors.lastname: Mizel
authors.forename: Y
authors.initials: Y
authors.lastname: Qin
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.forename: J
authors.initials: J
authors.lastname: Schnermann
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000235:genetics / Q000502:physiology*; D005919:Glomerular Filtration Rate / Q000235:genetics / Q000502:physiology; D006339:Heart Rate / Q000235:genetics / Q000502:physiology*; D007668:Kidney / Q000378:metabolism*; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D043682:Receptor, Adenosine A1 / Q000172:deficiency / Q000378:metabolism*; D012083:Renin / Q000378:metabolism
chemical_list: D043682:Receptor, Adenosine A1; D012083:Renin
keywords: C57Bl/6 mice; SWR/J mice; adenosine; blood pressure telemetry; micropuncture