title: Detection of parathion and patulin by quartz-crystal microbalance functionalized by the photonics immobilization technique.
abstract: Oriented antibodies are tethered on the gold surface of a quartz crystal microbalance through the photonics immobilization technique so that limit of detection as low as 50 nM and 140 nM are achieved for parathion and patulin, respectively. To make these small analytes detectable by the microbalance, they have been weighed down through a "sandwich protocol" with a second antibody. The specificity against the parathion has been tested by checking the immunosensor response to a mixture of compounds similar to parathion, whereas the specificity against the patulin has been tested with a real sample from apple puree. In both cases, the results are more than satisfactory suggesting interesting outlook for the proposed device.
authors.affiliation: CNISM and Dipartimento di Fisica, Università di Napoli "Federico II", Via Cintia, 26, Naples 80126, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Funari
authors.affiliation: CNISM and Dipartimento di Fisica, Università di Napoli "Federico II", Via Cintia, 26, Naples 80126, Italy.
authors.forename: Bartolomeo
authors.initials: B
authors.lastname: Della Ventura
authors.affiliation: Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Unità di Ricerca per le Colture Alternative al Tabacco, Via P. Vitiello, 108, Scafati 84018, Italy.
authors.forename: Raffaele
authors.initials: R
authors.lastname: Carrieri
authors.affiliation: Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Unità di Ricerca per le Colture Alternative al Tabacco, Via P. Vitiello, 108, Scafati 84018, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Morra
authors.affiliation: Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Unità di Ricerca per le Colture Alternative al Tabacco, Via P. Vitiello, 108, Scafati 84018, Italy.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Lahoz
authors.affiliation: CNISM and Dipartimento di Fisica, Università di Napoli "Federico II", Via Cintia, 26, Naples 80126, Italy.
authors.forename: Felice
authors.initials: F
authors.lastname: Gesuele
authors.affiliation: CNISM and Dipartimento di Fisica, Università di Napoli "Federico II", Via Cintia, 26, Naples 80126, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Altucci
authors.affiliation: CNISM and Dipartimento di Fisica, Università di Napoli "Federico II", Via Cintia, 26, Naples 80126, Italy. Electronic address: rvelotta@unina.it.
authors.forename: Raffaele
authors.initials: R
authors.lastname: Velotta
mesh_terms: D004784:Environmental Monitoring / Q000295:instrumentation*; D004785:Environmental Pollutants / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D010278:Parathion / Q000032:analysis*; D010365:Patulin / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004785:Environmental Pollutants; D010278:Parathion; D010365:Patulin
keywords: Immunosensor; Parathion; Patulin; Photonics immobilization technique; Quartz crystal microbalance