title: Argonaute high-throughput sequencing of RNAs isolated by cross-linking immunoprecipitation reveals a snapshot of miRNA gene regulation in the mammalian retina.
abstract: Mounting evidence points to roles for miRNA gene regulation in promoting development, function, and cell survival in the mammalian retina. However, little is known regarding which retinal genes are targets of miRNAs. Here, we employed a systematic, nonbiased, biochemical approach to identify targets of miRNA gene regulation in the bovine retina, a common model species for vision research. Using Argonaute high-throughput sequencing of RNAs isolated by cross-linking immunoprecipitation analysis, we identified 348 high-confidence miRNA target sites within 261 genes. This list was enriched in rod and cone photoreceptor genes and included 28 retinal disease genes, providing further evidence of a role of miRNAs in the pathology of blinding diseases.
authors.affiliation: Department of Pharmacology and Cleveland Center for Membrane and Structural Biology and ‡Center for RNA Molecular Biology, Case Western Reserve University, School of Medicine , 2109 Adelbert Road, Cleveland, Ohio 44106, United States.
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Sundermeier
authors.forename: Hui
authors.initials: H
authors.lastname: Jin
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Kleinjan
authors.forename: Debarshi
authors.initials: D
authors.lastname: Mustafi
authors.forename: Donny D
authors.initials: DD
authors.lastname: Licatalosi
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Palczewski
mesh_terms: D000818:Animals; D060565:Argonaute Proteins / Q000276:immunology*; D002417:Cattle; D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D047468:Immunoprecipitation / Q000379:methods*; D035683:MicroRNAs / Q000235:genetics*; D012160:Retina / Q000502:physiology*
chemical_list: D060565:Argonaute Proteins; D035683:MicroRNAs