title: Isolation of microsatellite markers for the red mangrove, Rhizophora mangle (Rhizophoraceae).
abstract: OBJECTIVE
  Three species of the mangrove tree genus Rhizophora are found in the New World and along the west coast of Africa. Of these, R. mangle is the most abundant and has a complex interbreeding relationship with the sympatric R. racemosa and R. harrisonii. The development of additional microsatellite markers would permit paternity analyses and investigation of the hybrid origin of these species. •
  
  
  RESULTS
  Using an enriched library method, via hybridization with biotinylated oligonucleotides complementary to repetitive poly AG/TC, primers for 11 microsatellite markers of R. mangle were developed and characterized in populations in Pará and São Paulo (Brazil) and Florida (USA). Ten of these markers were transferable to R. racemosa and R. harrisonii. •
  
  
  CONCLUSIONS
  The microsatellite markers presented here will be useful in studies of contemporary and historical gene flow between American and West African Rhizophora species.
authors.affiliation: Institute for Coastal Research, Universidade Federal do Pará (UFPA), Alameda Leandro Ribeiro s/n, Aldeia, 68600-000 Bragança, Pará, Brazil.
authors.forename: Diana O
authors.initials: DO
authors.lastname: Ribeiro
authors.affiliation: Molecular Biology and Biochemistry, Universidade Federal de Viçosa, Viçosa, 36570-000 Minas Gerais, Brazil.
authors.forename: Christina C
authors.initials: CC
authors.lastname: Vinson
authors.affiliation: Institute for Coastal Research, Universidade Federal do Pará (UFPA), Alameda Leandro Ribeiro s/n, Aldeia, 68600-000 Bragança, Pará, Brazil.
authors.forename: Dulcivania S S
authors.initials: DS
authors.lastname: Nascimento
authors.affiliation: Institute for Coastal Research, Universidade Federal do Pará (UFPA), Alameda Leandro Ribeiro s/n, Aldeia, 68600-000 Bragança, Pará, Brazil.
authors.forename: Ulf
authors.initials: U
authors.lastname: Mehlig
authors.affiliation: Institute for Coastal Research, Universidade Federal do Pará (UFPA), Alameda Leandro Ribeiro s/n, Aldeia, 68600-000 Bragança, Pará, Brazil.
authors.forename: Moirah P M
authors.initials: MP
authors.lastname: Menezes
authors.affiliation: Institute for Coastal Research, Universidade Federal do Pará (UFPA), Alameda Leandro Ribeiro s/n, Aldeia, 68600-000 Bragança, Pará, Brazil.
authors.forename: Iracilda
authors.initials: I
authors.lastname: Sampaio
authors.affiliation: Institute for Coastal Research, Universidade Federal do Pará (UFPA), Alameda Leandro Ribeiro s/n, Aldeia, 68600-000 Bragança, Pará, Brazil.
authors.forename: Marivana B
authors.initials: MB
authors.lastname: Silva
keywords: Rhizophora harrisonii; Rhizophora racemosa; Rhizophoraceae; mangrove; microsatellites