title: Molecular characterisation of four class 2 cytokine receptor family members in rainbow trout, Oncorhynchus mykiss.
abstract: The interleukin (IL)-10 cytokine family includes IL-10, IL-19, IL-20, IL-22, IL-24, IL-26 and the lambda/type III interferons. They are highly pleiotropic and mediate a variety of activities, including immune suppression and antibacterial immunity. To exert their functions they signal through a heterodimeric receptor composed of a subunit with a long intracellular domain (R1 type receptors; IL-10R1, IL-20R1 or IL-22R1) and a subunit with a short intracellular domain (R2 type receptors; IL-10R2 or IL-20R2). In this study we report the identification of three R1 type receptors (named IL-10R1/CRFB7, IL-20R1a/CRFB8a and IL-20R1b/CRFB8b) and one R2 type receptor (named IL-10R2/CRFB4) in rainbow trout. The nomenclature of the receptors was supported by homology analysis, conserved motifs and phylogenetic tree analysis, confirming they belong to the piscine class 2 cytokine receptor family. For instance, they all displayed the presence of characteristic features, such as conserved fibronectin type-III domains. Expression analysis in tissues collected from healthy fish revealed different patterns of expression for each receptor, suggesting their potential involvement in different types of immune responses. When studying the modulation of the genes in cell lines and primary cultures, a greater effect was observed in the cell lines, where the expression of most receptors was affected by incubation with microbial mimics (LPS and PolyI:C) or the pro-inflammatory cytokine rIFN-γ. In addition, expression of the four receptors was modulated by viral infection, suggesting a potential involvement of such receptors and their ligands in antiviral defence.
authors.affiliation: Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, Scotland, UK. Electronic address: m.monte@abdn.ac.uk.
authors.forename: Milena M
authors.initials: MM
authors.lastname: Monte
authors.affiliation: Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, Scotland, UK.
authors.forename: Tiehui
authors.initials: T
authors.lastname: Wang
authors.affiliation: Marine Scotland Science, 375 Victoria Road, Aberdeen AB11 9DB, Scotland, UK.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Collet
authors.affiliation: Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, Scotland, UK.
authors.forename: Jun
authors.initials: J
authors.lastname: Zou
authors.affiliation: Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, Scotland, UK.
authors.forename: Chris J
authors.initials: CJ
authors.lastname: Secombes
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D005393:Fish Diseases / Q000276:immunology* / Q000821:virology; D005786:Gene Expression Regulation; D016753:Interleukin-10 / Q000235:genetics*; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000276:immunology*; D061251:Primary Cell Culture; D017434:Protein Structure, Tertiary; D018121:Receptors, Cytokine / Q000096:biosynthesis / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D018121:Receptors, Cytokine; D016753:Interleukin-10
keywords: Cell lines; Class 2 cytokine receptor family (CRF2); Head kidney leucocytes; In vitro stimulation; Oncorhynchus mykiss; Viral infection