title: PKA phosphorylation of cardiac troponin I modulates activation and relaxation kinetics of ventricular myofibrils.
abstract: Protein kinase A (PKA) phosphorylation of myofibril proteins constitutes an important pathway for β-adrenergic modulation of cardiac contractility and relaxation. PKA targets the N-terminus (Ser-23/24) of cardiac troponin I (cTnI), cardiac myosin-binding protein C (cMyBP-C) and titin. The effect of PKA-mediated phosphorylation on the magnitude of contraction has been studied in some detail, but little is known about how it modulates the kinetics of thin filament activation and myofibril relaxation as Ca(2+) levels vary. Troponin C (cTnC) interaction with cTnI (C-I interaction) is a critical step in contractile activation that can be modulated by cTnI phosphorylation. We tested the hypothesis that altering C-I interactions by PKA, or by cTnI phosphomimetic mutations (S23D/S24D-cTnI), directly affects thin filament activation and myofilament relaxation kinetics. Rat ventricular myofibrils were isolated and endogenous cTn was exchanged with either wild-type cTnI, or S23D/S24D-cTnI recombinant cTn. Contractile mechanics were monitored at maximum and submaximal Ca(2+) concentrations. PKA treatment of wild-type cTn or exchange of cTn containing S23D/S24D-cTnI resulted in an increase in the rate of early, slow phase of relaxation (kREL,slow) and a decrease in its duration (tREL,slow). These effects were greater for submaximal Ca(2+) activated contractions. PKA treatment also reduced the rate of contractile activation (kACT) at maximal, but not submaximal Ca(2+), and reduced the Ca(2+) sensitivity of contraction. Using a fluorescent probe coupled to cTnC (C35S-IANBD), the Ca(2+)-cTn binding affinity and C-I interaction were monitored. Ca(2+) binding to cTn (pCa50) was significantly decreased when cTnI was phosphorylated by PKA (ΔpCa50 = 0.31). PKA phosphorylation of cTnI also weakened C-I interaction in the presence of Ca(2+). These data suggest that weakened C-I interaction, via PKA phosphorylation of cTnI, may slow thin filament activation and result in increased myofilament relaxation kinetics, the latter of which could enhance early phase diastolic relaxation during β-adrenergic stimulation.
authors.affiliation: University of Washington, Department of Bioengineering, Seattle, Washington.
authors.forename: Vijay
authors.initials: V
authors.lastname: Rao
authors.affiliation: University of Washington, Department of Bioengineering, Seattle, Washington; National Biomedical Computational Resource, La Jolla, California.
authors.forename: Yuanhua
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: University of California San Diego, Department of Pharmacology, La Jolla, California.
authors.forename: Steffen
authors.initials: S
authors.lastname: Lindert
authors.affiliation: University of Washington, Department of Bioengineering, Seattle, Washington.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: University of Washington, Department of Bioengineering, Seattle, Washington.
authors.forename: Lucas
authors.initials: L
authors.lastname: Oxenford
authors.affiliation: University of California San Diego, Department of Bioengineering, La Jolla, California; National Biomedical Computational Resource, La Jolla, California.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: McCulloch
authors.affiliation: University of California San Diego, Department of Pharmacology, La Jolla, California; National Biomedical Computational Resource, La Jolla, California.
authors.forename: J Andrew
authors.initials: JA
authors.lastname: McCammon
authors.affiliation: University of Washington, Department of Bioengineering, Seattle, Washington. Electronic address: mregnier@uw.edu.
authors.forename: Michael
authors.initials: M
authors.lastname: Regnier
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D007477:Ions / Q000378:metabolism; D007700:Kinetics; D009154:Mutation; D009200:Myocardial Contraction / Q000502:physiology*; D009210:Myofibrils / Q000502:physiology*; D010766:Phosphorylation; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D019209:Troponin C / Q000378:metabolism; D019210:Troponin I / Q000378:metabolism*
chemical_list: D007477:Ions; D011994:Recombinant Proteins; D019209:Troponin C; D019210:Troponin I; D017868:Cyclic AMP-Dependent Protein Kinases; D002118:Calcium