title: Integron-borne transmission of VEB-1 extended-spectrum β-lactamase in Pseudomonas aeruginosa in a tertiary care hospital in India.
abstract: A total 14 clinical isolates of Pseudomonas aeruginosa that produced VEB-1 and were susceptible only to polymyxin B were recovered from hospitalized patients. VEB-1 was located within variable regions of the class 1 integron, flanked by resistant genes, and was horizontally transferable as well as carried within the IncP-type plasmid. We conclude that the IncP-type plasmid is responsible for the horizontal transmission of VEB-1-mediated expanded-spectrum cephalosporin resistance in this medical center.
authors.affiliation: Department of Microbiology, Assam University, Silchar, India.
authors.forename: Anand Prakash
authors.initials: AP
authors.lastname: Maurya
authors.affiliation: Department of Life Science and Bioinformatics, Assam University, Silchar, India.
authors.forename: Anupam Das
authors.initials: AD
authors.lastname: Talukdar
authors.affiliation: Department of Microbiology, Silchar Medical College and Hospital, Silchar, India.
authors.forename: Debadatta Dhar
authors.initials: DD
authors.lastname: Chanda
authors.affiliation: Department of Microbiology, Silchar Medical College and Hospital, Silchar, India.
authors.forename: Atanu
authors.initials: A
authors.lastname: Chakravarty
authors.affiliation: Department of Microbiology, Assam University, Silchar, India ab0404@gmail.com.
authors.forename: Amitabha
authors.initials: A
authors.lastname: Bhattacharjee
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002511:Cephalosporins / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D029968:Escherichia coli Proteins; D022761:Gene Transfer, Horizontal / Q000235:genetics*; D006801:Humans; D007194:India; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D011112:Polymyxin B / Q000627:therapeutic use; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D062606:Tertiary Care Centers; D063128:Tertiary Health Care; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C119424:beta-lactamase VEB-1, E coli; D001618:beta-Lactamases; D011112:Polymyxin B