title: Astrocytic and vascular scaffolding for neuroblast migration in the rostral migratory stream.
abstract: New neurons are continuously being added to the olfactory bulb (OB) of adult rodents that are generated in the subventricular zone (SVZ), distant by a few millimeters. Neuronal precursors have to overcome this long distance without the radial-glial migratory scaffold, in contrast to migration mode during embryonic development. The previous model explains migration of precursors from the SVZ through the rostral migratory stream (RMS) to the OB as a movement of neuroblasts along each other, ensheathed by astroglial tubes. Recent results indicate that blood vessels are suitable candidates for neuronal migration guidance in the RMS. These novel findings have changed the former concept accounting for neuronal precursor migration. The aim of our study was to map a pattern of vascularization in the RMS of adult rats and to investigate mutual relations among blood vessels, neuroblasts and astrocytes in this area. Detailed morphological analysis revealed that blood vessels in the RMS are organized in a specific manner. In most of the RMS extent, blood vessels run parallel to the outline of the migratory pathway. Interestingly, the caudal part of the RMS has a unique vasculature organization in which blood vessels create a spiral-like configuration. Chains of neuroblasts enveloped by astrocytes largely align along blood vessels. The exception is the caudal part of the RMS where neuroblasts do not follow non - parallel blood vessels. Our morphological findings suggest that blood vessels and astrocytes may cooperatively form physical substrate - scaffold for the neuroblasts migration in the RMS of adult rats.
authors.forename: Marcela
authors.initials: M
authors.lastname: Martoncikova
authors.forename: Kamila
authors.initials: K
authors.lastname: Fabianova
authors.forename: Andrea
authors.initials: A
authors.lastname: Schreiberova
authors.forename: Juraj
authors.initials: J
authors.lastname: Blasko
authors.forename: Viera
authors.initials: V
authors.lastname: Almasiova
authors.affiliation: Institute of Neurobiology, Slovak Academy of Sciences, Soltesovej 4, 040 01 Kosice, Slovak Republic. martoncikova@saske.sk.
authors.forename: Eniko
authors.initials: E
authors.lastname: Racekova
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001253:Astrocytes / Q000502:physiology*; D001808:Blood Vessels / Q000166:cytology*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002465:Cell Movement / Q000502:physiology*; D002552:Cerebral Ventricles / Q000166:cytology / Q000502:physiology; D000089622:Doublecortin Domain Proteins; D005260:Female; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D058953:Neural Stem Cells / Q000502:physiology*; D009479:Neuropeptides / Q000378:metabolism; D009830:Olfactory Bulb / Q000166:cytology / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D054457:Tissue Scaffolds
chemical_list: D015815:Cell Adhesion Molecules; D000089622:Doublecortin Domain Proteins; C568780:Esam protein, rat; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides