title: Anoctamin-1 in the juvenile rat urinary bladder.
abstract: OBJECTIVE
  To investigate presence, location and functional role of calcium-activated chloride channel (CaCC) Anoctamin-1 (Ano1) in rat urinary bladder.
  
  
  METHODS
  Bladders from 3 week old Wistar rats were studied. End-point PCR on total mRNA was used to assess the expression of Ano1. Immunofluorescent labelling of whole mount bladder tissue imaged with confocal microscope allowed localization of Ano1 and vimentin immunopositive cells. The effects of CaCC blockers: niflumic acid (NFA) (3,10,30 µM) and 5-Nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) (10, 30 µM) on spontaneous phasic contractile activity of intact (with mucosa) and denuded (without mucosa) detrusor strips were measured under isometric tension in organ baths (n = 141, N = 60).
  
  
  RESULTS
  Ano1 expression was found at mRNA level in mucosa and detrusor layers. Confocal microscopy revealed presence of Ano1 immunopositive cells in mucosa and in detrusor layers; a subpopulation of vimentin positive cells expressed Ano1. Both chloride channel blockers reduced the amplitude and frequency of phasic contractions in denuded and intact strips.
  
  
  CONCLUSIONS
  Ano1 is expressed in rat urinary bladder and is present in cells sharing markers with interstitial cells. CaCC blockers reduced phasic activity of the bladder tissue. Ano1 is expressed in the bladder and plays a role in its spontaneous phasic contractile activity.
authors.affiliation: School of Clinical Sciences, University of Bristol, Bristol, United Kingdom; Bristol Urological Institute, North Bristol NHS Trust, Bristol, United Kingdom.
authors.forename: Dominika A
authors.initials: DA
authors.lastname: Bijos
authors.affiliation: School of Clinical Sciences, University of Bristol, Bristol, United Kingdom; Bristol Urological Institute, North Bristol NHS Trust, Bristol, United Kingdom.
authors.forename: Marcus J
authors.initials: MJ
authors.lastname: Drake
authors.affiliation: Bristol Urological Institute, North Bristol NHS Trust, Bristol, United Kingdom; Department of Biological, Biomedical and Analytical Sciences, University of the West of England, Bristol, United Kingdom.
authors.forename: Bahareh
authors.initials: B
authors.lastname: Vahabi
mesh_terms: D000375:Aging; D000818:Animals; D000075369:Anoctamin-1; D018118:Chloride Channels / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D049990:Membrane Transport Modulators / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects; D009544:Niflumic Acid / Q000494:pharmacology; D009579:Nitrobenzoates / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism
chemical_list: C552942:ANO1 protein, rat; D000075369:Anoctamin-1; D018118:Chloride Channels; D049990:Membrane Transport Modulators; D009579:Nitrobenzoates; D012333:RNA, Messenger; D014746:Vimentin; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D009544:Niflumic Acid