title: Antibacterial and cytotoxic effect of biologically synthesized silver nanoparticles using aqueous root extract of Erythrina indica lam.
abstract: Simple, yet an effective and rapid approach for the green synthesis of silver nanoparticles (Ag NPs) using root extract of Erythrina indica and its in vitro antibacterial activity was tried against human pathogenic bacteria and its cytotoxic effect in breast and lung cancer cell lines has been demonstrated in this study. Various instrumental techniques were adopted to characterize the synthesized Ag NPs viz. UV-Vis (Ultra violet), FTIR (Fourier Transform Infrared), XRD (X-ray diffraction), DLS (Dynamic light scattering), HR TEM (High-resolution transmission electron microscopy), EDX (Energy-dispersive X-ray spectroscopy). Surface plasmon spectra for Ag NPs are centered nearly at 438 nm with dark brown color. FTIR analysis revealed the presence of terpenes, phenol, flavonols and tannin act as effective reducing and capping agents for converting silver nitrate to Ag NPs. The synthesized Ag NPs were found to be spherical in shape with size in the range of 20-118 nm. Moreover, the synthesized Ag NPs showed potent antibacterial activity against Gram positive and Gram negative bacteria and these biologically synthesized nanoparticles were also proved to exhibit excellent cytotoxic effect on breast and lung cancer cell lines.
authors.affiliation: Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India. Electronic address: rathissk@gmail.com.
authors.forename: P R
authors.initials: PR
authors.lastname: Rathi Sre
authors.affiliation: Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Reka
authors.affiliation: Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India.
authors.forename: R
authors.initials: R
authors.lastname: Poovazhagi
authors.affiliation: Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Arul Kumar
authors.affiliation: Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India.
authors.forename: K
authors.initials: K
authors.lastname: Murugesan
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D004895:Erythrina / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D008027:Light; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D012542:Scattering, Radiation; D012834:Silver / Q000494:pharmacology*; D013052:Spectrometry, X-Ray Emission; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D014867:Water; D012834:Silver
keywords: Antibacterial; Biosynthesis; Characterization; Cytotoxicity; E. indica root; Silver nanoparticles