title: Development of microsatellite loci for Cyclocarya paliurus (Juglandaceae), a monotypic species in subtropical China.
abstract: OBJECTIVE
  Microsatellite loci were developed for a monotypic species endemic to subtropical China, Cyclocarya paliurus, to help infer the evolutionary histories of ancient monotypic genera in subtropical China. •
  
  
  RESULTS
  Using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, 28 primer sets were identified in two wild populations. All loci were polymorphic, with the number of alleles per locus ranging from two to eight. The expected and observed heterozygosities ranged from 0.153 to 0.802 and from 0 to 0.750, respectively. The transferability of the 28 primer pairs was tested on Juglans regia, Pterocarya stenoptera, and Platycarya strobilacea. Twenty-one (75.0%), 22 (78.6%), and 15 (53.6%) markers were successfully amplified in J. regia, P. stenoptera, and P. strobilacea, respectively. •
  
  
  CONCLUSIONS
  These loci will be useful for in-depth analysis of genetic diversity and phylogeographical variation throughout the distribution range of C. paliurus.
authors.affiliation: Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, People's Republic of China.
authors.forename: Deng-Mei
authors.initials: DM
authors.lastname: Fan
authors.affiliation: Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, People's Republic of China.
authors.forename: Lin-Jiang
authors.initials: LJ
authors.lastname: Ye
authors.affiliation: Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, People's Republic of China.
authors.forename: Wan
authors.initials: W
authors.lastname: Hu
authors.affiliation: Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, People's Republic of China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Tian
authors.affiliation: Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, People's Republic of China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Zhang
keywords: Cyclocarya paliurus; Juglandaceae; microsatellite marker; population genetics