title: A unique pattern of bisphenol a effects on nerve growth factor gene expression in embryonic mouse hypothalamic cell line N-44.
abstract: We investigated the toxicity of bisphenol A (BPA) by determining the gene expression of nerve growth factor (Ngf in the embryonic mouse cell line mHypoE-N44 derived from the hypothalamus exposed to BPA dose range between 0.02 and 200 μmol L-1 for 3 h. Ngf mRNA levels decreased in a dose-dependent manner, with significant reductions observed in the 2 to 50 μmol L-1 BPA treatment groups compared to controls. However, at 100 to 200 μmol L-1 the NgfmRNA gradually increased and was significantly higher than control, while the expression of the apoptosis-related genes Caspase 3 and transformation-related protein 73 decreased significantly. These results suggest that in an embryonic hypothalamic cell line the higher doses of BPA induce a unique pattern of Ngf gene expression and that BPA has the potential to suppress apoptosis essential for early-stage brain development.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Warita
authors.forename: Tomoko
authors.initials: T
authors.lastname: Mitsuhashi
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Hoshi
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Ohta
authors.forename: Shingo
authors.initials: S
authors.lastname: Suzuki
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: Takanori
authors.initials: T
authors.lastname: Miki
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics*; D001559:Benzhydryl Compounds / Q000633:toxicity*; D002460:Cell Line / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects*; D007031:Hypothalamus / Q000187:drug effects* / Q000254:growth & development; D051379:Mice; D020932:Nerve Growth Factor / Q000187:drug effects* / Q000235:genetics*; D010636:Phenols / Q000633:toxicity*; D012333:RNA, Messenger / Q000187:drug effects; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D020932:Nerve Growth Factor; D010636:Phenols; D012333:RNA, Messenger; C006780:bisphenol A; D000098826:Bisphenol A Compounds