title: Phylogenetic analysis of poly and non structural protein in Japanese Encephalitis virus with other related viral families.
abstract: BACKGROUND
  Japanese encephalitis (JE) causes inflammation of brain. The mortality rate due to JE is 30% while 10 -15 % of patients make full recovery. The disease spreads through infected mosquito bites breeding in rice fields and feeds on pigs, birds, and ducks.
  
  
  OBJECTIVE
  As proteins show important structure to function relationship the study was designed to carry out the identification of poly and non-structural proteins in the infective virus group using different strains of Japanese encephalitis virus i.e. JAOARS982, Nakayama Strain SA (V), Strain SA-14.
  
  
  METHODS
  With reference to non structural proteins we obtained protein sequences of the following Japanese encephalitis virus groups: Japanese encephalitis virus, Weatnile virus, Kunjin virus. Further comparative and phylogenetic analysis was performed to explore evolutionary relationship among these groups.
  
  
  RESULTS
  Results of phylogeny of alignment score was found to be 375184 using multiple alignment, Jal view, ClustalW (1.83) and ClustalW2. However, the analysis among the non-structural proteins of Japanese Encephalitis Virus, Westnile Virus, and Kunjin Virus revealed the phylogeny alignment score to be 875 through multiple sequence alignment and Tree view respectively.
  
  
  CONCLUSIONS
  Phylogenetic analysis revealed that these four strains are interrelated as well as showing high similarity with the other viruses of this group due to conserved regions among their sequences.
authors.affiliation: Amity Institute of Biotechnology, Amity University Lucknow Campus, UP;
authors.forename: Parachi
authors.initials: P
authors.lastname: Srivastava
authors.affiliation: Amity Institute of Biotechnology, Amity University Lucknow Campus, UP;
authors.forename: Ashish Chandra
authors.initials: AC
authors.lastname: Trivedi
authors.affiliation: Amity Institute of Biotechnology, Amity University Lucknow Campus, UP;
authors.forename: Anshul
authors.initials: A
authors.lastname: Tiwari
authors.affiliation: Department of Microbiology, International Medical &' Technological University, Dares-Salaam, TANZANIA;
authors.forename: Alpha
authors.initials: A
authors.lastname: Verma
authors.affiliation: Invitro Toxicology division, Indian Institute of Toxicology Research, Lucknow, UP.
authors.forename: A B
authors.initials: AB
authors.lastname: Pant
keywords: Japanese Encephalitis; Multiple Sequence Alignment; Phylogenetic Analysis