title: Effect of EDTA preparations on rotary root canal instrumentation.
abstract: BACKGROUND
  The aim of this study was to evaluate whether rotary instrumentation using saline, EDTA 17% solution, or RC-Prep (Premier Dental, Philadelphia, PA) resulted in differences in root canal transportation. The secondary objective was to assess if instrumentation using these agents caused changes in the working length and canal volume.
  
  
  METHODS
  Moderately curved mesiobuccal roots of 24 maxillary molars were standardized in length and randomized into 1 control and 2 experimental groups. The canals were instrumented with 0.04 taper rotary files to size #30. All groups were irrigated with saline. Group 1 was also irrigated using EDTA 17% solution (Pulpdent Corp, Watertown, MA), and in group 2, RC-Prep was used. X-ray micro-computed tomographic scans and working length measurements were made before and after instrumentation. Three-dimensional models were created from the pre- and postinstrumentation scan data and compared for volume changes. Centroid points were calculated in cross-sectional slices of the canals, and transportation was determined by measuring the distance between the pre- and postinstrumentation points. The data were analyzed with 1-way analysis of variance (α = 0.05) and the Tukey post hoc test.
  
  
  RESULTS
  Less transportation was observed in group 2 than in group 1 (P = .001) and the control group (P = .014). Transportation in group 1 and the control group was not significantly different. Canal volume in group 1 was increased relative to group 2 (P = .004) and the control group (P = .022). No significant differences in the working length were observed.
  
  
  CONCLUSIONS
  The use of chelating agents during root canal instrumentation did not significantly increase apical transportation.
authors.affiliation: Naval Postgraduate Dental School, Bethesda, Maryland. Electronic address: whitbeck.evan@gmail.com.
authors.forename: Evan R
authors.initials: ER
authors.lastname: Whitbeck
authors.affiliation: Naval Postgraduate Dental School, Bethesda, Maryland.
authors.forename: Kelli
authors.initials: K
authors.lastname: Swenson
authors.affiliation: Naval Postgraduate Dental School, Bethesda, Maryland.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Tordik
authors.affiliation: Naval Postgraduate Dental School, Bethesda, Maryland.
authors.forename: Shayne A
authors.initials: SA
authors.lastname: Kondor
authors.affiliation: Naval Postgraduate Dental School, Bethesda, Maryland.
authors.forename: Terry D
authors.initials: TD
authors.lastname: Webb
authors.affiliation: Dr Anthony Volpe Research Center, American Dental Association Foundation, Gaithersburg, Maryland.
authors.forename: Jirun
authors.initials: J
authors.lastname: Sun
mesh_terms: D016022:Case-Control Studies; D002614:Chelating Agents / Q000737:chemistry; D003722:Dental Alloys; D003762:Dental Instruments; D003786:Dental Pulp Cavity; D004492:Edetic Acid / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D008963:Molar; D009532:Nickel / Q000737:chemistry; D010545:Peroxides / Q000737:chemistry; D011897:Random Allocation; D012388:Root Canal Irrigants / Q000737:chemistry; D018915:Root Canal Preparation / Q000295:instrumentation* / Q000379:methods; D012390:Root Canal Therapy / Q000295:instrumentation* / Q000379:methods; D014025:Titanium / Q000737:chemistry; D019227:Tooth Apex; D014092:Tooth Root; D014508:Urea / Q000737:chemistry; D014885:Waxes / Q000737:chemistry
chemical_list: D002614:Chelating Agents; D003722:Dental Alloys; D010545:Peroxides; D012388:Root Canal Irrigants; D014885:Waxes; C040654:titanium nickelide; C025081:RC-Prep; D009532:Nickel; D014508:Urea; D004492:Edetic Acid; D014025:Titanium
keywords: Chelating agents; EDTA; root canal preparation; transportation; x-ray micro–computed tomographic imaging