title: [Isolation and identification of cellulolytic anaerobic fungi and their associated methanogens from holstein cow].
abstract: OBJECTIVE
  We studied the microbial interaction between anaerobic fungi and methanogens in the rumen of Holstein Cow.
  
  
  METHODS
  Co-cultures of anaerobic fungi with indigenously associated methanogen were isolated by Hungate roll-tube technique. The anaerobic fungi were identified by morphology and 4', 6 diamidino-2-phylindole nucleus staining and the methanogens were identified by 16S rRNA gene sequencing.
  
  
  RESULTS
  A total of 28 co-cultures of anaerobic fungus with indigenously associated methanogen were obtained. The anaerobic fungi in the co-cultures were identified as monocentric genera Piromyces, Neocallimastix and Caeomyces. The indigenously associated methanogens were Methanobrevibacter olleyae like and Methanobrevibacter thaueri like strains. Four different phylotypes of fungus-methanogen co-cultures were obtained, which were Piromyces/Methanobrevibacter olleyae like strains, Neocallimastix/ Methanobrevibacter olleyae like strains, Neocallimastix/Methanobrevibacter thaueri like strains and Caecomyces/ Methanobrevibacter olleyae like strains.
  
  
  CONCLUSIONS
  Our study isolated and identified 28 co-cultures of anaerobic fungus and associated methanogens, which provided new materials for further study the mechanism of methane emission in the rumen.
authors.forename: Meizhou
authors.initials: M
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.forename: Yuanfei
authors.initials: Y
authors.lastname: Li
authors.forename: Shengyong
authors.initials: S
authors.lastname: Mao
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Cheng
authors.forename: Weiyun
authors.initials: W
authors.lastname: Zhu
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D002417:Cattle; D002482:Cellulose / Q000378:metabolism*; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D008697:Methane / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012417:Rumen / Q000382:microbiology*
chemical_list: D002482:Cellulose; D008697:Methane