title: Keratinocyte growth factor, interleukins (1 beta, 6, 8, 10, 12), and tumor necrosis factor alpha in culture medium of dermal fibroblast of burned patients.
abstract: OBJECTIVE
  To evaluate the level of cytokines and keratinocyte growth factor (KGF) or Fibroblast Growth Factor 7 (FGF-7) in the culture medium of cultured human dermal fibroblasts from patients with large burn in comparison to small burn.
  
  
  METHODS
  Fibroblasts of 10 patients (four large burns, four small burns and two controls) were initiated by the enzymatic method using collagenase. Cytokines and KGF in the supernatant of the culture medium was measured by, respectively, flow cytometry using Cytometric Bead Array Human Inflammation kit (CBA, BD Biosciences, USA) and the enzyme immunoassay method using the Quantikine (r) Human KGF. The experiments were performed in triplicate.
  
  
  RESULTS
  The expression of IL-12 protein in patients with large burns showed a tendency to increase. IL- 6, IL- 10, and IL- 1beta were observed no difference. For IL - 8, TNF - alpha and KGF was observed a significant difference between the expression in large and small burned patient.
  
  
  CONCLUSIONS
  That IL-8, TNF-alpha and KGF showed higher expression in cultured fibroblasts of large burned patients.
authors.affiliation: Department of Surgery, EPM, UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Samuel Marcos Ribeiro de
authors.initials: SM
authors.lastname: Noronha
authors.affiliation: Department of Surgery, EPM, UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Silvana Aparecida Alves Corrêa de
authors.initials: SA
authors.lastname: Noronha
authors.affiliation: EPM, UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Anthony Gueratto
authors.initials: AG
authors.lastname: Klepp
authors.affiliation: Department of Gynecology, EPM, UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Michele Zampieri
authors.initials: MZ
authors.lastname: Ipolito
authors.affiliation: Department of Surgery, EPM, UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Lydia Masako
authors.initials: LM
authors.lastname: Ferreira
authors.affiliation: Department of Surgery, Paulista School of Medicine, Federal University of Sao Paulo, Brazil.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Gragnani
mesh_terms: D000328:Adult; D002056:Burns / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051523:Fibroblast Growth Factor 7 / Q000032:analysis* / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007378:Interleukins / Q000032:analysis* / Q000378:metabolism; D008297:Male; D012867:Skin / Q000293:injuries* / Q000473:pathology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000378:metabolism
chemical_list: D003470:Culture Media; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; D051523:Fibroblast Growth Factor 7