title: Expression of hypoxia-inducible factor 1 alpha and oligodendrocyte lineage gene-1 in cultured brain slices after oxygen-glucose deprivation.
abstract: Oligodendrocyte lineage gene-1 expressed in oligodendrocytes may trigger the repair of neuronal myelin impairment, and play a crucial role in myelin repair. Hypoxia-inducible factor 1α, a transcription factor, is of great significance in premature infants with hypoxic-ischemic brain damage. There is little evidence of direct regulatory effects of hypoxia-inducible factor 1α on oligodendrocyte lineage gene-1. In this study, brain slices of Sprague-Dawley rats were cultured and subjected to oxygen-glucose deprivation. Then, slices were transfected with hypoxia-inducible factor 1α or oligodendrocyte lineage gene-1. The expression levels of hypoxia-inducible factor 1α and oligodendrocyte lineage gene-1 were significantly up-regulated in rat brains prior to transfection, as detected by immunohistochemical staining. Eight hours after transfection of slices with hypoxia-inducible factor 1α, oligodendrocyte lineage gene-1 expression was upregulated, and reached a peak 24 hours after transfection. Oligodendrocyte lineage gene-1 transfection induced no significant differences in hypoxia-inducible factor 1α levels in rat brain tissues with oxygen-glucose deprivation. These experimental findings indicate that hypoxia-inducible factor 1α can regulate oligodendrocyte lineage gene-1 expression in hypoxic brain tissue, thus repairing the neural impairment.
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang 050016, Hebei Province, China ; Department of Pediatrics, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Cui
authors.affiliation: Department of Pediatrics, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Weijuan
authors.initials: W
authors.lastname: Han
authors.affiliation: Department of Pediatrics, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Yang
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang 050016, Hebei Province, China.
authors.forename: Yanzhong
authors.initials: Y
authors.lastname: Chang
keywords: biological factors; brain injury; brain slice culture; grants-supported paper; hypoxia-inducible factor 1α; immunohistochemistry; myelin repair; neural regeneration; neuroregeneration; oligodendrocyte; oligodendrocyte lineage gene-1; oxygen-glucose deprivation; photographs-containing paper; premature delivery; rat