title: Mouse monoclonal antibodies against estrogen receptor.
abstract: The production of monoclonal antibodies, by cloning hybridoma derived from the fusion of myeloma cells and spleen lymphocytes, has allowed to obtain great advances in many fields of biological knowledge. The use of specific antibodies to the estrogen receptor, in fact, has been an invaluable method to bring out its mechanisms of action and its effects, both genomic and extra-genomic. Here we describe, step by step, the production of monoclonal antibodies, starting from protocol for antigen preparation to the selection of antibody-secreting hybridoma.
authors.affiliation: Dipartimento di Biochimica, Biofisica e Patologia Generale-II Seconda Università di Napoli, Via L. De Crecchio 7, Naples, 80138, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: De Rosa
authors.forename: Valentina
authors.initials: V
authors.lastname: Rossi
authors.forename: Ciro
authors.initials: C
authors.lastname: Abbondanza
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods; D002459:Cell Fusion; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D047628:Estrogen Receptor alpha / Q000276:immunology; D005260:Female; D006825:Hybridomas / Q000166:cytology / Q000276:immunology*; D007114:Immunization; D015151:Immunoblotting / Q000379:methods; D007124:Immunoenzyme Techniques / Q000379:methods; D061986:MCF-7 Cells; D051379:Mice; D011960:Receptors, Estrogen / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D011960:Receptors, Estrogen