title: Isolation of antileishmanial, antimalarial and antimicrobial metabolites from Jatropha multifida.
abstract: OBJECTIVE
  To investigate the antileishmanial, antimicrobial and antimalarial activities of the pure metabolites from Jatropha multifida used in African ethnomedicine.
  
  
  METHODS
  The methanolic stem bark extract of Jatropha multifida used in Nigerian folk medicine as remedy against bacterial infections was subjected to column chromatography and HPLC analyses to obtain three known metabolites, microcyclic lathyrane diterpenoids (1-3). Structures were confirmed by comparison of 1D and 2D spectral data with literature.
  
  
  RESULTS
  The three compounds exhibited inhibition of antileishmanial, antimalarial and antimicrobial actions against the tested organisms with compouds 2 and 3 active against Cryptococcus neoformans at IC50 of 8.2 and 8.7 µg/mL, respectively.
  
  
  CONCLUSIONS
  The research lends support to the ethnomedicinal use of the plant in combating microbial infections, leishmaniasis and malarial infections.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, Benin City, 300001, Nigeria ; Department of Pharmacognosy, School of Pharmacy, University of Mississippi, MS 38655, USA.
authors.forename: Abiodun
authors.initials: A
authors.lastname: Falodun
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, Benin City, 300001, Nigeria ; Institute of Organic Chemistry, University of Rostock, 18509, Rostock, Germany.
authors.forename: Vincent
authors.initials: V
authors.lastname: Imieje
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, Benin City, 300001, Nigeria ; Institute of Organic Chemistry, University of Rostock, 18509, Rostock, Germany.
authors.forename: Osayewenre
authors.initials: O
authors.lastname: Erharuyi
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, Benin City, 300001, Nigeria.
authors.forename: Ahomafor
authors.initials: A
authors.lastname: Joy
authors.affiliation: Institute of Organic Chemistry, University of Rostock, 18509, Rostock, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Langer
authors.affiliation: National Center for Natural Products Research, Institute of Pharmaceutical Sciences, University of Mississippi, 38677, USA.
authors.forename: Melissa
authors.initials: M
authors.lastname: Jacob
authors.affiliation: National Center for Natural Products Research, Institute of Pharmaceutical Sciences, University of Mississippi, 38677, USA.
authors.forename: Shabanna
authors.initials: S
authors.lastname: Khan
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, University of Mississippi, MS 38655, USA.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Abaldry
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, University of Mississippi, MS 38655, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Hamann
keywords: Antimalarial; Antimicrobial; Jatropha multifida; Leishmaniasis; Stem bark