title: Quantitative estimation of hesperidin by HPTLC in different varieties of citrus peels.
abstract: OBJECTIVE
  To develop a simple, selective, sensitive and accurate high-performance thin layer chromatography (HPTLC) method to determine the quantity of hesperidin in different varieties of citrus fruits.
  
  
  METHODS
  The method was carried out in aluminum-backed silica gel 60 F254 plates with ethyl acetate-methanol-water 15:3:2 (%, v/v) as mobile phase.
  
  
  RESULTS
  A compact band was obtained for hesperidin at Rf value of (0.40±0.04). The calibration plot was linear in the range of 100-800 ng/spot of hesperidin and the correlation coefficient of 0.998 6 was indicative of good linear dependence of peak area on concentration. Limit of detection (8.87 ng/spot), limit of quantification (23.21 ng/spot), accuracy (less than 2%) and recovery (ranging from 98.55-99.38) were found satisfactory.
  
  
  CONCLUSIONS
  The method developed can be used for routine analysis of hesperidin in crude drug as well as in herbal and pharmaceutical dosage form containing citrus fruits as an ingredient.
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Kingdom of Saudi Arabia.
authors.forename: Prawez
authors.initials: P
authors.lastname: Alam
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Kingdom of Saudi Arabia.
authors.forename: Aftab
authors.initials: A
authors.lastname: Alam
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, Salman Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Kingdom of Saudi Arabia.
authors.forename: Md Khalid
authors.initials: MK
authors.lastname: Anwer
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Kingdom of Saudi Arabia.
authors.forename: Saleh I
authors.initials: SI
authors.lastname: Alqasoumi
keywords: Citrus; HPTLC; Hesperidin