title: Influence of static magnetic fields combined with human insulin-like growth factor 1 on human satellite cell cultures.
abstract: Tissue engineering represents a promising research field, targeting the creation of new functional muscle tissue in vitro. The aim of the present study was to show the influence of static magnetic fields (SMF) and insulin-like growth factor-1 (IGF1), as enhancing stimuli on human satellite cell cultures, which are preferred sources of stem cells in engineering skeletal muscle tissue. To detect effects on myogenic maturation and proliferation, AlamarBlue® proliferation, assay and semi-quantitative reverse transcription-polymerase chain reaction of following markers was performed: desmin (DES), myogenic factor-5 (MYF5), myogenic differentiation antigen-1 (MYOD1), myogenin (MYOG), myosin heavy chain (MYH) and α1 actin (ACTA1). As a distinct marker of differentiation, immunohistochemical staining and fusion index determination was performed on satellite cell cultures stimulated with IGF1 and IGF1-plus-SMF with an intensity of 80 mT. Proliferation was increased by additional SMF application to IGF1-stimulated cell cultures on the first day of myogenesis. Relative gene expression of measured markers was increased by IGF1 application in the first days of myogenesis except for ACTA1. Additional SMF application enhanced this effect. Nevertheless we were unable to demonstrate the formation of contractile muscle tissue. Immunhistochemical staining verified muscle origin and all markers were displayed.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany richard.birk@medma.uni-heidelberg.de.
authors.forename: Richard
authors.initials: R
authors.lastname: Birk
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
authors.forename: J Ulrich
authors.initials: JU
authors.lastname: Sommer
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
authors.forename: Dominik
authors.initials: D
authors.lastname: Haas
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Faber
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Aderhold
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
authors.forename: Johannes D
authors.initials: JD
authors.lastname: Schultz
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
authors.forename: Karl
authors.initials: K
authors.lastname: Hoermann
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Stern-Straeter
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D060526:Magnetic Fields; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*
chemical_list: D007334:Insulin-Like Growth Factor I
keywords: Skeletal muscle; differentiation static magnetic fields; insulin-like-1 growth factor; satellite cells; tissue engineering