title: [Construction and expression of the targeting super-antigen EGF-SEA fusion gene].
abstract: OBJECTIVE
  To construct expression vector for the SEA-EGF fusion gene.
  
  
  METHODS
  Clone the SEA gene and the EGF gene segment with PCR and RT-PCR independently, and connect this two genes by the bridge PCR. Insert the fusion gene EGF-SEA into the expression vector PET-44. Induced the secretion of the fusion protein SEA-EGF by the antileptic.
  
  
  RESULTS
  The gene fragment encoding EGF and SEA mature peptide was successfully cloned. The fusion gene EGF-SEA was successfully constructed and was inserted into expression vector.
  
  
  CONCLUSIONS
  The new recombinant expression vector for fusion gene EGF-SEA is specific for head and neck cancer, laid the foundation for the further study of fusion protein SEA-EGF targeting immune therapy in head and neck tumors.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xie
authors.forename: Shaoping
authors.initials: S
authors.lastname: Peng
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Liao
authors.forename: Jiafeng
authors.initials: J
authors.lastname: Liu
authors.forename: Xuemei
authors.initials: X
authors.lastname: Liu
authors.forename: Weifeng
authors.initials: W
authors.lastname: Chen
mesh_terms: D004768:Enterotoxins / Q000235:genetics*; D004815:Epidermal Growth Factor / Q000235:genetics*; D005822:Genetic Vectors; D006258:Head and Neck Neoplasms / Q000188:drug therapy; D006801:Humans; D058990:Molecular Targeted Therapy; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004768:Enterotoxins; D011993:Recombinant Fusion Proteins; C026066:enterotoxin A, Staphylococcal; D004815:Epidermal Growth Factor