title: Characterization of the wzc gene from Pantoea sp. strain PPE7 and its influence on extracellular polysaccharide production and virulence on Pleurotus eryngii.
abstract: To characterize of the pathogenicity gene from the soft rot pathogen Pantoea sp. PPE7 in Pleurotus eryngii, we constructed over 10,000 kanamycin-resistant transposon mutants of Pantoea sp. strain PPE7 by transposon mutagenesis. One mutant, Pantoea sp. NPPE9535, did not cause a soft rot disease on Pleurotus eryngii was confirmed by the pathogenicity test. The transposon was inserted into the wzc gene and the disruption of the wzc gene resulted in the reduction of polysaccharide production and abolished the virulence of Pantoea sp. strain PPE7 in P. eryngii. Analysis of the hydropathic profile of this protein indicated that it is composed of two main domains: an N-terminal domain including two transmembrane α-helices and a C-terminal cytoplasmic domain consisting of a tyrosine-rich region. Comparative analysis indicated that the amino acid sequence of Wzc is similar to that of a number of proteins involved in the synthesis or export of polysaccharides in other bacterial species. Purified GST-Wzc was found to affect the phosphorylation of tyrosine residue in vivo. These results showed that the wzc gene might play an important role in the virulence of Pantoea sp. strain PPE7 in P. eryngii.
authors.affiliation: Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research and Extension Service, Jinju 660-360, Republic of Korea. Electronic address: goguma99@korea.kr.
authors.forename: Min Keun
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research and Extension Service, Jinju 660-360, Republic of Korea.
authors.forename: Young Han
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea.
authors.forename: Hyeran
authors.initials: H
authors.lastname: Kim
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea.
authors.forename: Jeongyeo
authors.initials: J
authors.lastname: Lee
authors.affiliation: Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research and Extension Service, Jinju 660-360, Republic of Korea.
authors.forename: Jae San
authors.initials: JS
authors.lastname: Ryu
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001483:Base Sequence; D004251:DNA Transposable Elements; D004789:Enzyme Activation; D062610:Fungal Polysaccharides / Q000096:biosynthesis*; D015723:Gene Library; D023061:Gene Order; D005816:Genetic Complementation Test; D008565:Membrane Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D056265:Microbial Interactions; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D020636:Pantoea / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D010641:Phenotype; D020076:Pleurotus / Q000378:metabolism* / Q000472:pathogenicity* / Q000648:ultrastructure; D011505:Protein-Tyrosine Kinases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D062610:Fungal Polysaccharides; D008565:Membrane Proteins; C417168:WZC protein, Bacteria; D011505:Protein-Tyrosine Kinases
keywords: Extracellular polysaccharides; Pleurotus eryngii; Protein-tyrosine kinase; wzc gene