title: New microsatellite markers for wild and commercial species of Passiflora (Passifloraceae) and cross-amplification.
abstract: OBJECTIVE
  We developed the first microsatellites for Passiflora setacea and characterized new sets of markers for P. edulis and P. cincinnata, enabling further genetic diversity studies to support the conservation and breeding of passion fruit species. •
  
  
  RESULTS
  We developed 69 microsatellite markers and, in conjunction with assessments of cross-amplification using primers available from the literature, present 43 new polymorphic microsatellite loci for three species of Passiflora. The mean number of alleles per locus was 3.1, and the mean values of the expected and observed levels of heterozygosity were 0.406 and 0.322, respectively. •
  
  
  CONCLUSIONS
  These microsatellite markers will be valuable tools for investigating the genetic diversity and population structure of wild and commercial species of passion fruit (Passiflora spp.) and may be useful for developing conservation and improvement strategies by contributing to the understanding of the mating system and hybridization within the genus.
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, Instituto de Biologia, Universidade Estadual de Campinas, CP 6010, 13083-875 Campinas, São Paulo, Brazil ; Departamento de Ciências Exatas e Naturais, Universidade Estadual do Sudoeste da Bahia, 45700-000 Itapetinga, Bahia, Brazil.
authors.forename: Carlos B M
authors.initials: CB
authors.lastname: Cerqueira-Silva
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, Instituto de Biologia, Universidade Estadual de Campinas, CP 6010, 13083-875 Campinas, São Paulo, Brazil ; Departamento de Ciências Exatas e Naturais, Universidade Estadual do Sudoeste da Bahia, 45700-000 Itapetinga, Bahia, Brazil.
authors.forename: Elisa S L
authors.initials: ES
authors.lastname: Santos
authors.affiliation: Departamento de Ciências Exatas e Naturais, Universidade Estadual do Sudoeste da Bahia, 45700-000 Itapetinga, Bahia, Brazil.
authors.forename: João G P
authors.initials: JG
authors.lastname: Vieira
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, Instituto de Biologia, Universidade Estadual de Campinas, CP 6010, 13083-875 Campinas, São Paulo, Brazil.
authors.forename: Gustavo M
authors.initials: GM
authors.lastname: Mori
authors.affiliation: Empresa Brasileira de Pesquisa Agropecuária, Centro Nacional de Pesquisa em Mandioca e Fruticultura Tropical, 44380-000 Cruz das Almas, Bahia, Brazil.
authors.forename: Onildo N
authors.initials: ON
authors.lastname: Jesus
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna Km 16, 45662-900 Ilhéus, Bahia, Brazil.
authors.forename: Ronan X
authors.initials: RX
authors.lastname: Corrêa
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, Instituto de Biologia, Universidade Estadual de Campinas, CP 6010, 13083-875 Campinas, São Paulo, Brazil ; Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, 13083-875 Campinas, São Paulo, Brazil.
authors.forename: Anete P
authors.initials: AP
authors.lastname: Souza
keywords: Passiflora; genetic diversity; genomic microsatellite-enriched library; molecular markers; simple sequence repeats; wild passion fruit