title: Development of microsatellites for Verbenoxylum reitzii (Verbenaceae), a tree endemic to the Brazilian Atlantic Forest.
abstract: OBJECTIVE
  Microsatellite markers were developed for Verbenoxylum reitzii (Verbenaceae), a tree endemic to the Brazilian Atlantic Forest, to investigate their usefulness in population genetic studies. The loci were tested for cross-amplification in the related genera Recordia and Duranta. •
  
  
  RESULTS
  Eleven polymorphic microsatellite markers were isolated from an enriched library of V. reitzii and characterized. The primers were tested on 60 individuals from three populations of this species. The number of alleles per locus ranged from two to 11, and the observed and expected heterozygosities varied from 0.0 to 1.0 and from 0.088 to 0.758, respectively. Ten loci successfully amplified in R. boliviana and all failed in D. vestita. •
  
  
  CONCLUSIONS
  Our results suggest the usefulness of the microsatellite loci developed here to access genetic variability for phylogeographic and population genetic studies in V. reitzii, which are important for the conservation of this rare species.
authors.affiliation: Programa de Pós-Graduação em Botânica, Universidade Federal do Rio Grande do Sul, Bento Gonçalves 9500, Porto Alegre, Brazil ; Laboratory of Molecular Evolution, Department of Genetics, Universidade Federal do Rio Grande do Sul, PO Box 15053, Porto Alegre, Brazil.
authors.forename: Verônica A
authors.initials: VA
authors.lastname: Thode
authors.affiliation: Laboratory of Molecular Evolution, Department of Genetics, Universidade Federal do Rio Grande do Sul, PO Box 15053, Porto Alegre, Brazil.
authors.forename: Alice
authors.initials: A
authors.lastname: Backes
authors.affiliation: Laboratory of Molecular Evolution, Department of Genetics, Universidade Federal do Rio Grande do Sul, PO Box 15053, Porto Alegre, Brazil.
authors.forename: Geraldo
authors.initials: G
authors.lastname: Mader
authors.affiliation: Laboratory of Molecular Evolution, Department of Genetics, Universidade Federal do Rio Grande do Sul, PO Box 15053, Porto Alegre, Brazil.
authors.forename: Raquel
authors.initials: R
authors.lastname: Kriedt
authors.affiliation: Genomic and Molecular Biology Laboratory, Pontifícia Universidade Católica do Rio Grande do Sul, Ipiranga 6681, Porto Alegre, Brazil.
authors.forename: Sandro L
authors.initials: SL
authors.lastname: Bonatto
authors.affiliation: Programa de Pós-Graduação em Botânica, Universidade Federal do Rio Grande do Sul, Bento Gonçalves 9500, Porto Alegre, Brazil ; Laboratory of Molecular Evolution, Department of Genetics, Universidade Federal do Rio Grande do Sul, PO Box 15053, Porto Alegre, Brazil.
authors.forename: Loreta B
authors.initials: LB
authors.lastname: Freitas
keywords: Recordia; Verbenaceae; Verbenoxylum; Verbenoxylum reitzii; cross-amplification; microsatellite