title: [Magnetic beads adsorption-PCR detection method of four common foodborne pathogens in food].
abstract: OBJECTIVE
  To evaluate the adsorption capacity of magnetic beads for common foodborne pathogens.
  
  
  METHODS
  Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Cronobacter sakazakii were used as targets, the suitable conditions were obtained by optimization the incubation time, bead size, reaction temperature and so on. The adsorption efficiency of the magnetic beads in analog samples was also tested.
  
  
  RESULTS
  The optimum conditions were as follows: 2.0 - 3.0 microm diameter magnetic beads 50 microl, incubation for 60 min under 37 degrees C. In analog samples, the adsorption efficiency of the magnetic beads for Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Cronobacter sakazakii in juice, milk, duck meat were 50% - 84%, 46% - 66%, 54% - 74%, respectively.
  
  
  CONCLUSIONS
  Magnetic beads enrichment technique has high adsorption efficiency, simple to operate, which is meet the requirement for PCR detection.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Hu
authors.forename: Xue
authors.initials: X
authors.lastname: Tian
authors.forename: Hairong
authors.initials: H
authors.lastname: Yang
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Zhao
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000327:Adsorption; D000818:Animals; D044083:Cronobacter sakazakii; D003611:Dairy Products; D004926:Escherichia coli; D005506:Food Contamination; D005516:Food Microbiology; D008089:Listeria monocytogenes; D060328:Magnetic Phenomena; D008460:Meat; D008892:Milk; D016133:Polymerase Chain Reaction; D013211:Staphylococcus aureus