title: Synthetic seed production and physio-biochemical studies in Cassia angustifolia Vahl. - a medicinal plant.
abstract: Synthetic seed technology is an alternative to traditional micropropagation for production and delivery of cloned plantlets. Synthetic seeds were produced by encapsulating nodal segments of C. angustifolia in calcium alginate gel. 3% (w/v) sodium alginate and 100 mM CaCl2 · 2H2O were found most suitable for encapsulation of nodal segments. Synthetic seeds cultured on half strength Murashige and Skoog medium supplemented with thidiazuron (5.0 μM) + indole-3-acetic acid (1.0 μM) produced maximum number of shoots (10.9 ± 0.78) after 8 weeks of culture exhibiting (78%) in vitro conversion response. Encapsulated nodal segments demonstrated successful regeneration after different period (1-6 weeks) of cold storage at 4 °C. The synthetic seeds stored at 4 °C for a period of 4 weeks resulted in maximum conversion frequency (93%) after 8 weeks when placed back to regeneration medium. The isolated shoots when cultured on half strength Murashige and Skoog medium supplemented with 1.0 μM indole-3-butyric acid (IBA), produced healthy roots and plantlets with well-developed shoot and roots were successfully hardened off in plastic pots containing sterile soilrite inside the growth chamber and gradually transferred to greenhouse where they grew well with 85% survival rate. Growth performance of 2 months old in vitro-raised plant was compared with in vivo seedlings of the same age. Changes in the content of photosynthetic pigments, net photosynthetic rate (PN), superoxide dismutase and catalase activity in C. angustifolia indicated the adaptation of micropropagated plants to ex vitro conditions.
authors.affiliation: King Saud University Department of Botany and Microbiology, College of Science, Female Centre for Scientific and Medical Colleges P.O. Box 22452 Riyadh 11495 Saudi Arabia.
authors.forename: N A W
authors.initials: NA
authors.lastname: Bukhari
authors.affiliation: King Saud University Department of Botany and Microbiology, College of Science, Female Centre for Scientific and Medical Colleges P.O. Box 22452 Riyadh 11495 Saudi Arabia.
authors.forename: Iram
authors.initials: I
authors.lastname: Siddique
authors.affiliation: King Saud University Department of Botany and Microbiology, College of Science, Female Centre for Scientific and Medical Colleges P.O. Box 22452 Riyadh 11495 Saudi Arabia.
authors.forename: K
authors.initials: K
authors.lastname: Perveen
authors.affiliation: King Saud University Department of Botany and Microbiology, College of Science, Female Centre for Scientific and Medical Colleges P.O. Box 22452 Riyadh 11495 Saudi Arabia.
authors.forename: I
authors.initials: I
authors.lastname: Siddiqui
authors.affiliation: King Saud University Department of Botany and Microbiology, College of Science, Female Centre for Scientific and Medical Colleges P.O. Box 22452 Riyadh 11495 Saudi Arabia.
authors.forename: M S
authors.initials: MS
authors.lastname: Alwahibi
mesh_terms: D000222:Adaptation, Physiological; D002374:Catalase / Q000378:metabolism; D049109:Cell Proliferation; D002734:Chlorophyll / Q000378:metabolism; D010788:Photosynthesis; D010937:Plant Growth Regulators / Q000494:pharmacology; D010946:Plants, Medicinal / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D012038:Regeneration; D012639:Seeds / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D029846:Senna Plant / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D013482:Superoxide Dismutase; D013997:Time Factors; D046509:Tissue Culture Techniques
chemical_list: D010937:Plant Growth Regulators; D002734:Chlorophyll; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Antioxidant enzymes; Thidiazuron; encapsulation; rooting; synthetic seeds