title: Direct roles of SPEECHLESS in the specification of stomatal self-renewing cells.
abstract: Lineage-specific stem cells are critical for the production and maintenance of specific cell types and tissues in multicellular organisms. In Arabidopsis, the initiation and proliferation of stomatal lineage cells is controlled by the basic helix-loop-helix transcription factor SPEECHLESS (SPCH). SPCH-driven asymmetric and self-renewing divisions allow flexibility in stomatal production and overall organ growth. How SPCH directs stomatal lineage cell behaviors, however, is unclear. Here, we improved the chromatin immunoprecipitation (ChIP) assay and profiled the genome-wide targets of Arabidopsis SPCH in vivo. We found that SPCH controls key regulators of cell fate and asymmetric cell divisions and modulates responsiveness to peptide and phytohormone-mediated intercellular communication. Our results delineate the molecular pathways that regulate an essential adult stem cell lineage in plants.
authors.affiliation: Department of Biology, Stanford University, Stanford, CA 94305, USA.
authors.forename: On Sun
authors.initials: OS
authors.lastname: Lau
authors.affiliation: Department of Biology, Stanford University, Stanford, CA 94305, USA.
authors.forename: Kelli A
authors.initials: KA
authors.lastname: Davies
authors.affiliation: Department of Biology, Stanford University, Stanford, CA 94305, USA.
authors.forename: Jessica
authors.initials: J
authors.lastname: Chang
authors.affiliation: Department of Biology, Stanford University, Stanford, CA 94305, USA.
authors.forename: Jessika
authors.initials: J
authors.lastname: Adrian
authors.affiliation: Department of Biology, Stanford University, Stanford, CA 94305, USA.
authors.forename: Matthew H
authors.initials: MH
authors.lastname: Rowe
authors.affiliation: Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Ballenger
authors.affiliation: Department of Biology, Stanford University, Stanford, CA 94305, USA. Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA. Carnegie Institution for Science, Stanford, CA 94305, USA. dbergmann@stanford.edu.
authors.forename: Dominique C
authors.initials: DC
authors.lastname: Bergmann
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology*; D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D002450:Cell Communication / Q000187:drug effects / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D019070:Cell Lineage / Q000187:drug effects / Q000235:genetics; D047369:Chromatin Immunoprecipitation; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant / Q000235:genetics; D010937:Plant Growth Regulators / Q000494:pharmacology / Q000502:physiology; D054046:Plant Stomata / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D010937:Plant Growth Regulators; C517480:SPEECHLESS protein, Arabidopsis