title: Changes in oleic Acid content of transgenic soybeans by antisense RNA mediated posttranscriptional gene silencing.
abstract: The Delta-12 oleate desaturase gene (FAD2-1), which converts oleic acid into linoleic acid, is the key enzyme determining the fatty acid composition of seed oil. In this study, we inhibited the expression of endogenous Delta-12 oleate desaturase GmFad2-1b gene by using antisense RNA in soybean Williams 82. By employing the soybean cotyledonary-node method, a part of the cDNA of soybean GmFad2-1b 801 bp was cloned for the construction of a pCAMBIA3300 vector under the soybean seed promoter BCSP. Leaf painting, LibertyLink strip, PCR, Southern blot, qRT-PCR, and fatty acid analysis were used to detect the insertion and expression of GmFad2-1b in the transgenic soybean lines. The results indicate that the metabolically engineered plants exhibited a significant increase in oleic acid (up to 51.71%) and a reduction in palmitic acid (to <3%) in their seed oil content. No structural differences were observed between the fatty acids of the transgenic and the nontransgenic oil extracts.
authors.affiliation: Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, China.
authors.forename: Xiang-Dong
authors.initials: XD
authors.lastname: Yang
authors.affiliation: Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, China.
authors.forename: Yuan-Yu
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, China.
authors.forename: Guang-Xun
authors.initials: GX
authors.lastname: Qi
authors.affiliation: Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, China.
authors.forename: Dong-Quan
authors.initials: DQ
authors.lastname: Guo
authors.affiliation: Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, China.
authors.forename: Guo-Jie
authors.initials: GJ
authors.lastname: Xing
authors.affiliation: Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, China.
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Xu
authors.affiliation: Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, China.
authors.forename: Hai-Yun
authors.initials: HY
authors.lastname: Li
authors.affiliation: Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, China.
authors.forename: Qi-Yun
authors.initials: QY
authors.lastname: Li
authors.affiliation: Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun, Jilin 130033, China.
authors.forename: Ying-Shan
authors.initials: YS
authors.lastname: Dong