title: Haptens, bioconjugates, and antibodies for penthiopyrad immunosensing.
abstract: Haptens, bioconjugates, and antibodies for highly sensitive immunochemical analysis of the new-generation fungicide penthiopyrad are described. Two haptens with equivalent carboxylated linkers were prepared, and the purified active esters were efficiently coupled to proteins. The results revealed slightly different antibody-eliciting capacities for the two synthetic derivatives. All of the produced antibodies were specific for penthiopyrad, and showed affinity values in the nanomolar range.
authors.affiliation: Department of Organic Chemistry, Universitat de València, Doctor Moliner 50, 46100 Burjassot, València, Spain.
authors.forename: Eric
authors.initials: E
authors.lastname: Ceballos-Alcantarilla
authors.forename: Antonio
authors.initials: A
authors.lastname: Abad-Fuentes
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Aloisio
authors.forename: Consuelo
authors.initials: C
authors.lastname: Agulló
authors.forename: Antonio
authors.initials: A
authors.lastname: Abad-Somovilla
authors.forename: Josep V
authors.initials: JV
authors.lastname: Mercader
mesh_terms: D000906:Antibodies / Q000276:immunology*; D015374:Biosensing Techniques; D006241:Haptens; D018796:Immunoconjugates; D011720:Pyrazoles / Q000032:analysis* / Q000276:immunology; D013876:Thiophenes / Q000032:analysis* / Q000276:immunology
chemical_list: D000906:Antibodies; D006241:Haptens; D018796:Immunoconjugates; D011720:Pyrazoles; D013876:Thiophenes; C539106:penthiopyrad