title: Preparation and pharmacokinetics in beagle dogs of ganershu sustained-release pellets.
abstract: BACKGROUND
  The active ingredients of Ganershu compound recipe, which are effective for hepatitis treatment in liver protection and transaminase reduction. However, the active ingredients of Ganershu compound recipe are poor absorption, which conduct it has a low oral bioavailability.
  
  
  OBJECTIVE
  We prepared Ganershu sustained-release pellets (GSPs) by fluidized-bed on central composite design-response surface methodology and increase its bioavailability in beagle dogs.
  
  
  METHODS
  In this study, GSPs were successfully prepared. The Drug-loaded pellets and sustained-release coated were carried out in fluidized-bed machine. GSP was optimized for fitting release, roundness, and the overall desirability by central composite design-response surface methodology.
  
  
  RESULTS
  To optimize cumulative release profile, the outermost ethyl cellulose coating layer and the hydroxypropyl methyl cellulose (HPMC) swelling layer were employed, which were respectively given coating levels in terms of weight gain of 22% and 6%, the concentration of HPMC is 4.5% (g/ml). The pharmacokinetics of Ganershu normal pellets (GNPs) and GSP was studied in beagle dogs after oral administration. The naringenin as an index, the area under the curve0-∞ of naringenin in GSP was 1.38 times greater than that of GNP. Meanwhile, Tmax of GSP was prolonged for about 74%.
  
  
  CONCLUSIONS
  This study can clearly indicate that we enhanced the oral bioavailability of Ganershu by preparing the GSP, which had the sustained dissolution and improved the potential of it for clinical application.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China ; Department of Pharmacy Office, Traditional Chinese Medicine Hospital of Zhang-jia-gang, Zhangjiagang 215600, China.
authors.forename: Jin-Huo
authors.initials: JH
authors.lastname: Pan
authors.affiliation: Department of Pharmacy Office, Traditional Chinese Medicine Hospital of Zhang-jia-gang, Zhangjiagang 215600, China.
authors.forename: Jian-Chun
authors.initials: JC
authors.lastname: Wang
authors.affiliation: Department of Pharmacy Office, Traditional Chinese Medicine Hospital of Zhang-jia-gang, Zhangjiagang 215600, China.
authors.forename: Zhi-Tao
authors.initials: ZT
authors.lastname: Jiang
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Shao-Bo
authors.initials: SB
authors.lastname: Ge
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Ye-Xia
authors.initials: YX
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy Office, The First People's Hospital of Suqian, Suqian 223800, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Yan
keywords: Beagle dogs; Ganershu sustained-release pellets; fluidized-bed; naringenin; pharmacokinetics