title: Detection of the glucocorticoid receptors in brain protein extracts by SDS-PAGE.
abstract: Glucocorticoids are steroid hormones vital for organ system homeostasis and for the maintenance of essential biological processes. A significant part of these actions are mediated through glucocorticoid receptor (GR) that belongs to the nuclear receptor superfamily. To cover such variety of processes the different glucocorticoids act through different GR isoforms that are originated due to posttranscriptional and posttranslational mechanisms. For this reason when evaluating the levels of GRs we should preferentially determine protein levels instead of gene expression. Here, we describe the detection by Western blotting of the GR (α and β isoforms) protein, using macrodissected brain tissue.
authors.affiliation: Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus Gualtar, Braga, 4710-057, Portugal.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Marques
authors.forename: João C
authors.initials: JC
authors.lastname: Sousa
authors.forename: João J
authors.initials: JJ
authors.lastname: Cerqueira
authors.forename: Nuno
authors.initials: N
authors.lastname: Sousa
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D001923:Brain Chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008297:Male; D008810:Mice, Inbred C57BL; D011965:Receptors, Glucocorticoid / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D011965:Receptors, Glucocorticoid; C411470:glucocorticoid receptor alpha; C121943:glucocorticoid receptor beta