title: Development and evaluation of microsatellite markers for a native prairie perennial, Echinacea angustifolia (Asteraceae).
abstract: OBJECTIVE
  Microsatellite loci for the native prairie perennial Echinacea angustifolia were developed and evaluated for future use in population structure and paternity studies. •
  
  
  RESULTS
  A total of 50 trinucleotide microsatellite regions were identified though an enrichment protocol that prescreens for microsatellite repeats before ligating into a vector. Of these, 11 loci were polymorphic and in Hardy-Weinberg equilibrium in three populations with varying numbers of plants. The loci had between three and 14 alleles and collectively provided high paternity exclusion probabilities. •
  
  
  CONCLUSIONS
  These sets of microsatellite primers will provide researchers and land managers with valuable information on the population genetic structure and gene flow between fragmented prairie populations.
authors.affiliation: Department of Biological Sciences, University of Illinois at Chicago, 845 West Taylor Street, Chicago, Illinois 60607 USA ; Plant Conservation and Biology, Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, Illinois 60022 USA.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Ison
authors.affiliation: Plant Conservation and Biology, Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, Illinois 60022 USA.
authors.forename: Stuart
authors.initials: S
authors.lastname: Wagenius
authors.affiliation: Department of Biology, Carleton College, One North College Street, Northfield, Minnesota 55057 USA.
authors.forename: Diedre
authors.initials: D
authors.lastname: Reitz
authors.affiliation: Department of Biological Sciences, University of Illinois at Chicago, 845 West Taylor Street, Chicago, Illinois 60607 USA.
authors.forename: Mary V
authors.initials: MV
authors.lastname: Ashley
keywords: Asteraceae; Echinacea angustifolia; fragmentation; microsatellites; perennial herb; tallgrass prairie