title: Best practices for mapping replication origins in eukaryotic chromosomes.
abstract: Understanding the regulatory principles ensuring complete DNA replication in each cell division is critical for deciphering the mechanisms that maintain genomic stability. Recent advances in genome sequencing technology facilitated complete mapping of DNA replication sites and helped move the field from observing replication patterns at a handful of single loci to analyzing replication patterns genome-wide. These advances address issues, such as the relationship between replication initiation events, transcription, and chromatin modifications, and identify potential replication origin consensus sequences. This unit summarizes the technological and fundamental aspects of replication profiling and briefly discusses novel insights emerging from mining large datasets, published in the last 3 years, and also describes DNA replication dynamics on a whole-genome scale.
authors.affiliation: Laboratory of Genome Plasticity and Aging, Institute of Functional Genomics, CNRS UMR5203, INSERM U661, UMI, Montpellier, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Besnard
authors.forename: Romain
authors.initials: R
authors.lastname: Desprat
authors.forename: Michael
authors.initials: M
authors.lastname: Ryan
authors.forename: Malik
authors.initials: M
authors.lastname: Kahli
authors.forename: Mirit I
authors.initials: MI
authors.lastname: Aladjem
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Lemaitre
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D002875:Chromosomes / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication / Q000502:physiology*; D005057:Eukaryotic Cells / Q000378:metabolism*; D018741:Replication Origin / Q000502:physiology*
keywords: DNA replication; bioinformatics; next-generation sequencing (NGS); origins; replication timing