title: Construction of human Fab library and screening of a single-domain antibody of amyloid-beta 42 oligomers.
abstract: Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers. Thus, the present study prepared amyloid-beta 42 oli-gomers and constructed a naïve human Fab phage display library based on blood samples from six healthy people. After three rounds of biopanning in vitro, a human single-domain antibody that specifically recognized amyloid-beta 42 oligomers was identified. Western blot and enzyme-linked immunosorbent assay demonstrated this antibody bound specifically to human amyloid-beta 42 tetramer and nonamer, but not the monomer or high molecular weight oligomers. This study successfully constructed a human phage display library and screened a single-domain antibody that specifically recognized amyloid-beta 42 oligomers.
authors.affiliation: Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, Beijing Normal University, Beijing 100875, China ; Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Southeast University, Nanjing 210009, Jiangsu Province, China.
authors.forename: Zuanning
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, Beijing Normal University, Beijing 100875, China ; Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Southeast University, Nanjing 210009, Jiangsu Province, China.
authors.forename: Minge
authors.initials: M
authors.lastname: Du
authors.affiliation: Geriatric Internal Department of Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, Beijing Normal University, Beijing 100875, China ; Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Southeast University, Nanjing 210009, Jiangsu Province, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Dou
keywords: Alzheimer's disease; Parkinson's disease; alpha-synuclein; amyloid-beta; antibody library construction; grants-supported paper; humanized antibody; immunotherapy; neural regeneration; neuroregeneration; oligomer; phage display; single-domain antibody