title: Antitumor effects of dammarane-type saponins from steamed Notoginseng.
abstract: Six dammarane-type saponins were extracted from steamed Panax notoginseng. Their chemical structures were identified spectroscopically as ginsenosides Rh1 (1), Rg1 (2), 20 (S)-Rg3 (3), 20 (R)-Rg3 (4), Rb3 (5), and Rb1 (6). Compounds (0.1-10 μM) were tested for inhibition of tumor necrosis factor-α (TNF)-induced nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) luciferase reporter activity using a human kidney 293T cell-based assay. Ginsenoside Rb3 (5) showed the most significant activity with an IC50 of 8.2 μM. This compound also inhibited the induction of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) messenger Ribonucleic acid (mRNA) in a dose-dependent manner after HepG2 cells had been treated with TNF-α (10 ng/mL).
authors.affiliation: Department of Pharmacognosy, Liaoning University of Traditional Chinese Medicine, Dalian, China.
authors.forename: Fan
authors.initials: F
authors.lastname: He
authors.affiliation: Institute of Chemistry and Applications of Plant Resources, Dalian Polytechnic University, Dalian, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Herbal Medicine Improvement Research Center, Korea Institute of Oriental Medicine, Daejeon, Korea.
authors.forename: Chun
authors.initials: C
authors.lastname: Liang
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, Korea.
authors.forename: Seok Bean
authors.initials: SB
authors.lastname: Song
authors.affiliation: Department of Pharmacognosy, Liaoning University of Traditional Chinese Medicine, Dalian, China.
authors.forename: De-Qiang
authors.initials: DQ
authors.lastname: Dou
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, Korea.
authors.forename: Gyu Yong
authors.initials: GY
authors.lastname: Song
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, Korea.
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
keywords: Antitumor; Panax notoginseng; dammarane-type ginsenoside; inhibitory activity