title: A CRISPR view of development.
abstract: The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-associated nuclease 9) system is poised to transform developmental biology by providing a simple, efficient method to precisely manipulate the genome of virtually any developing organism. This RNA-guided nuclease (RGN)-based approach already has been effectively used to induce targeted mutations in multiple genes simultaneously, create conditional alleles, and generate endogenously tagged proteins. Illustrating the adaptability of RGNs, the genomes of >20 different plant and animal species as well as multiple cell lines and primary cells have been successfully modified. Here we review the current and potential uses of RGNs to investigate genome function during development.
authors.affiliation: Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53706, USA;
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Harrison
authors.affiliation: Biochemistry Department, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA;
authors.forename: Brian V
authors.initials: BV
authors.lastname: Jenkins
authors.affiliation: Laboratory of Genetics, Laboratory of Cell and Molecular Biology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
authors.forename: Kate M
authors.initials: KM
authors.lastname: O'Connor-Giles
authors.affiliation: Biochemistry Department, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA; wildonger@wisc.edu.
authors.forename: Jill
authors.initials: J
authors.lastname: Wildonger
mesh_terms: D000818:Animals; D064130:CRISPR-Associated Proteins / Q000378:metabolism*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D016678:Genome / Q000235:genetics*; D006801:Humans; D009154:Mutation / Q000235:genetics; D017393:RNA Editing / Q000235:genetics; D058727:RNA, Small Untranslated
chemical_list: D064130:CRISPR-Associated Proteins; D058727:RNA, Small Untranslated
keywords: CRISPR; Cas9; RNA-guided nuclease; development; genome editing; genome engineering