title: Establishment of cell lines derived from the genus Macaca through controlled expression of cell cycle regulators.
abstract: Nonhuman primates are useful animal models for the study of human diseases. However, the number of established cell lines from nonhuman primates is quite limited compared with the number established from other experimental animals. The establishment of nonhuman primate cell lines would allow drug testing on those cell lines before moving experiments into primates. In this study, we established nonhuman primate primary cell lines by introducing the genes for CDK4R24C, cyclin D1, and hTERT. These cell lines proliferated more rapidly than primary cells and bypassed cellular senescence. Karyotype analysis showed that the chromosome patterns were intact in the immortalized cell lines. Furthermore, we showed that the expression of introduced genes could be precisely controlled through the Tet-Off system with the addition of doxycycline. The present study shows that introduction of the CDK4R24C, cyclin D1, and hTERT genes are effective methods of establishing nonhuman primate cell lines.
authors.affiliation: Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-amamiyamachi, Aoba-ku, Sendai, 981-8555, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Kuroda
authors.forename: Tohru
authors.initials: T
authors.lastname: Kiyono
authors.forename: Takahiro
authors.initials: T
authors.lastname: Eitsuka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Isogai
authors.forename: Koichi
authors.initials: K
authors.lastname: Takahashi
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Donai
authors.forename: Emiko
authors.initials: E
authors.lastname: Isogai
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Fukuda
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D002461:Cell Line, Transformed; D049109:Cell Proliferation / Q000235:genetics; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000235:genetics; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D051358:Cyclin-Dependent Kinase 4 / Q000235:genetics / Q000378:metabolism; D004318:Doxycycline / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D015151:Immunoblotting; D059785:Karyotype; D016086:Lentivirus / Q000235:genetics; D008251:Macaca; D008297:Male; D020125:Mutation, Missense; D019098:Telomerase / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D018797:Cell Cycle Proteins; D019938:Cyclin D1; D051358:Cyclin-Dependent Kinase 4; D019098:Telomerase; D004318:Doxycycline
keywords: CDK4R24C; CYCLIN D1; MACAQUE MONKEY; NONHUMAN PRIMATE MODEL; hTERT