title: Systematic and efficient separation of 11 compounds from Rhizoma Chuanxiong via counter-current chromatography-solid phase extraction-counter-current chromatography hyphenation.
abstract: A counter-current chromatography (CCC)-solid phase extraction (SPE)-CCC system with high preparative capacity was used to realize rapid one-run systematic separation of natural products, in which two six-port valves and the SPE cartridge served as the interface. In the orthogonal separation system, equal column volumes of TEB-300A and TEB-300B were employed for the first dimension (1st-D) and second dimension (2nd-D), respectively. An optimized solid-phase column (25 mm × 10 mm i.d.) packed with Oasis HLB materials acted as the trapping SPE column. The analyte-focusing effect of the trapping column associated with the considerable preparative capacities of 1st-D and 2nd-D using totally different solvent systems significantly facilitated this one-run systematic separation of natural product. Therefore, this proposed approach was successfully applied to isolate chemical compounds from the crude extract of Rhizoma Chuanxiong. As a result, 11 compounds with widely different polarities were separated by running CCC for only one time. More importantly, this hyphenated strategy could serve as a rapid and efficient systematic pathway for the separation of natural products.
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China; Collaborative Innovation Center of Resource-conserving & Environment-friendly Society and Ecological Civilization, Changsha 410083, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.affiliation: The Third Xiangya Hospital, Central South University, Changsha 410083, China.
authors.forename: Jianda
authors.initials: J
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China; Collaborative Innovation Center of Resource-conserving & Environment-friendly Society and Ecological Civilization, Changsha 410083, China.
authors.forename: Jingang
authors.initials: J
authors.lastname: Yu
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China; Collaborative Innovation Center of Resource-conserving & Environment-friendly Society and Ecological Civilization, Changsha 410083, China.
authors.forename: Yixi
authors.initials: Y
authors.lastname: Xie
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China; Collaborative Innovation Center of Resource-conserving & Environment-friendly Society and Ecological Civilization, Changsha 410083, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Jiang
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China; Collaborative Innovation Center of Resource-conserving & Environment-friendly Society and Ecological Civilization, Changsha 410083, China. Electronic address: hyangchem@csu.edu.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China; Collaborative Innovation Center of Resource-conserving & Environment-friendly Society and Ecological Civilization, Changsha 410083, China. Electronic address: xqchen@csu.edu.cn.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Chen
mesh_terms: D000085:Acetates; D000097:Acetonitriles; D003377:Countercurrent Distribution / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D006586:Hexanes; D000432:Methanol; D052616:Solid Phase Extraction; D012997:Solvents; D014867:Water
chemical_list: D000085:Acetates; D000097:Acetonitriles; D004365:Drugs, Chinese Herbal; D006586:Hexanes; D012997:Solvents; D014867:Water; C026385:n-hexane; C007650:ethyl acetate; D000432:Methanol; C032159:acetonitrile
keywords: CCC–SPE–CCC; HSCCC; Rhizoma Chuanxiong; SPE; Systematic separation; Two dimensional HSCCC