title: Novel fluorinated lipopeptides from Bacillus sp. CS93 via precursor-directed biosynthesis.
abstract: While attempting to improve production of fluoro-iturin A in Bacillus sp. CS93 new mono- and di-fluorinated fengycins were detected in culture supernatants by (19)F NMR and tandem mass spectrometry, after incubation of the bacterium with 3-fluoro-L-tyrosine. The fluorinated amino acid was presumably incorporated in place of one or both of the tyrosyl residues in fengycin. Investigations to generate additional new fluorinated derivatives were undertaken using commercially available fluorinated phenylalanines and 2-fluoro- and 2,3-difluoro-tyrosine that were synthesised by Negishi cross-coupling of iodoalanine and fluorinated bromo-phenols. The anti-fungal activity of the fluorinated lipopeptides was assayed against Trichophyton rubrum and found to be similar to that of the non-fluorinated metabolites.
authors.affiliation: UCD School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Neil K
authors.initials: NK
authors.lastname: O'Connor
authors.forename: Alex S
authors.initials: AS
authors.lastname: Hudson
authors.forename: Steven L
authors.initials: SL
authors.lastname: Cobb
authors.forename: Deborah
authors.initials: D
authors.lastname: O'Neil
authors.forename: Jennifer
authors.initials: J
authors.lastname: Robertson
authors.forename: Vanessa
authors.initials: V
authors.lastname: Duncan
authors.forename: Cormac D
authors.initials: CD
authors.lastname: Murphy
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D001407:Bacillus / Q000737:chemistry / Q000378:metabolism*; D054879:Halogenation; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000096:biosynthesis* / Q000737:chemistry* / Q000494:pharmacology; D014249:Trichophyton / Q000187:drug effects; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D010456:Peptides, Cyclic; C001245:3-fluorotyrosine; D014443:Tyrosine; C013579:iturin A