title: Follicular fluid anti-Müllerian hormone: a predictive marker of fertilization capacity of MII oocytes.
abstract: OBJECTIVE
  The present study aimed to correlate anti-Müllerian hormone (AMH) levels in follicular fluid (FF) with oocyte maturity stages, morphological quality of metaphase II (MII) oocyte and fertilization capacity of MII oocytes.
  
  
  METHODS
  A total of 92 infertile women undergoing controlled ovarian stimulation and intracytoplasmic sperm injection were analyzed. Patients were divided into two groups according to age: <35 years (n = 43) and ≥35 years (n = 49). An FF sample was obtained from a single dominant follicle in each patient for a total of 92 follicular fluid samples analyzed. AMH levels in serum and follicular fluid were measured by enzyme-linked immunosorbent assay. Mature MII oocytes, zygotes, and embryos were assessed for morphological quality.
  
  
  RESULTS
  Serum AMH levels were significantly higher in patients aged <35 years. No correlation was observed between FF AMH level and oocyte maturation stages or morphological quality of MII oocyte. Significantly lower FF AMH levels were observed in fertilized MII oocytes than in non-fertilized MII oocytes in patients aged <35 years (2.56 ± 2.0 ng/ml vs. 4.81 ± 4.14 ng/ml; p = 0.032).
  
  
  CONCLUSIONS
  The present study revealed no correlation between FF AMH and oocyte maturity stage or morphological quality of MII oocyte. However, FF AMH might be a predictive marker for fertilization capacity of MII oocytes.
authors.affiliation: Department of Obstetrics and Gynecology, Clinical Hospital Center Rijeka, University of Rijeka, Cambierieva 17/5, 51 000, Rijeka, Croatia, tamtramisak@gmail.com.
authors.forename: Tamara
authors.initials: T
authors.lastname: Tramišak Milaković
authors.forename: Linda
authors.initials: L
authors.lastname: Panić Horvat
authors.forename: Kristina
authors.initials: K
authors.lastname: Čavlović
authors.forename: Neda
authors.initials: N
authors.lastname: Smiljan Severinski
authors.forename: Hrvoje
authors.initials: H
authors.lastname: Vlašić
authors.forename: Ivan
authors.initials: I
authors.lastname: Vlastelić
authors.forename: Dejan
authors.initials: D
authors.lastname: Ljiljak
authors.forename: Anđelka
authors.initials: A
authors.lastname: Radojčić Badovinac
mesh_terms: D000328:Adult; D000367:Age Factors; D054304:Anti-Mullerian Hormone / Q000032:analysis* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007247:Infertility, Female / Q000628:therapy; D008677:Metaphase; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D009866:Oogenesis; D006080:Ovarian Follicle / Q000378:metabolism*; D010062:Ovulation Induction; D011237:Predictive Value of Tests; D011247:Pregnancy; D018873:Pregnancy Rate; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D054304:Anti-Mullerian Hormone