title: In vitro cytotoxicity effects of date palm (Phoenix dactylifera L.) pollen on neonate mouse spermatogonial stem cells.
abstract: There is a fast growing tendency in the use of herbal remedies in developing countries. One of the traditional medicines used for male infertility treatment is date palm (Phoenix dactylifera) pollen (DPP). Isolated spermatogonial stem cells and sertoli cells using enzymatic digestion were grown in Dulbecco's modified Eagle's medium supplemented with 4% foetal bovine serum in the absence or presence of 0.06, 0.25 and 0.62 mg/mL concentrations of aqueous extract of DPP for 2 weeks. The assessment of mean number of the whole cells and the living cells showed that there were no significant differences between the mean viability percentage and proliferation rate between control and experimental groups (P>0.05). As there are no cytotoxicity effects of DPP in our cultural system, this system can be utilised for the enrichment or differentiation of these cells in clinical applications, cell replacement therapy, tissue regeneration and tissue engineering applications.
authors.affiliation: a Department of Molecular & Cellular Biology, Faculty of Biology , Damghan University , Semnan , Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Mahaldashtian
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Makoolati
authors.forename: Mohamad Taghi
authors.initials: MT
authors.lastname: Ghorbanian
authors.forename: Majid
authors.initials: M
authors.lastname: Naghdi
authors.forename: Seyed Amin
authors.initials: SA
authors.lastname: Kouhpayeh
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D008297:Male; D051379:Mice; D065749:Phoeniceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011058:Pollen / Q000737:chemistry*; D012708:Sertoli Cells / Q000187:drug effects*; D013093:Spermatogonia / Q000187:drug effects*; D013737:Testis / Q000166:cytology
chemical_list: D010936:Plant Extracts
keywords: date palm pollen; proliferation; spermatogonial stem cell; viability