title: Cross-species, amplifiable microsatellite markers for neoverrucid barnacles from deep-sea hydrothermal vents developed using next-generation sequencing.
abstract: Barnacles of the genus Neoverruca are abundant near deep-sea hydrothermal vents of the northwestern Pacific Ocean, and are useful for understanding processes of population formation and maintenance of deep-sea vent faunas. Using next-generation sequencing, we isolated 12 polymorphic microsatellite loci from Neoverruca sp., collected in the Okinawa Trough. These microsatellite loci revealed 2-19 alleles per locus. The expected and observed heterozygosities ranged from 0.286 to 1.000 and 0.349 to 0.935, respectively. Cross-species amplification showed that 9 of the 12 loci were successfully amplified for Neoverruca brachylepadoformis in the Mariana Trough. A pairwise FST value calculated using nine loci showed significant genetic differentiation between the two species. Consequently, the microsatellite markers we developed will be useful for further population genetic studies to elucidate genetic diversity, differentiation, classification, and evolutionary processes in the genus Neoverruca.
authors.affiliation: Marine Biophysics Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna, Okinawa 904-0495, Japan. yuichi.nakajima@oist.jp.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Nakajima
authors.affiliation: Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna, Okinawa 904-0495, Japan. c.shinzato@oist.jp.
authors.forename: Chuya
authors.initials: C
authors.lastname: Shinzato
authors.affiliation: Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna, Okinawa 904-0495, Japan. mariia.khalturina@oist.jp.
authors.forename: Mariia
authors.initials: M
authors.lastname: Khalturina
authors.affiliation: Department of Marine Biodiversity Research, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan. hwatanabe@jamstec.go.jp.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Watanabe
authors.affiliation: Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology, B-200 Monobe, Nankoku, Kochi 783-8502, Japan. inagaki@jamstec.go.jp.
authors.forename: Fumio
authors.initials: F
authors.lastname: Inagaki
authors.affiliation: Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna, Okinawa 904-0495, Japan. norisky@oist.jp.
authors.forename: Nori
authors.initials: N
authors.lastname: Satoh
authors.affiliation: Marine Biophysics Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna, Okinawa 904-0495, Japan. satoshi@oist.jp.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Mitarai
mesh_terms: D000818:Animals; D005828:Genetics, Population; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D060086:Hydrothermal Vents; D018895:Microsatellite Repeats / Q000235:genetics*; D001468:Thoracica / Q000235:genetics*