title: Microsatellite primers in the weedy annual herb Anacyclus clavatus (Asteraceae) and four closely related species.
abstract: OBJECTIVE
  Nuclear microsatellite primers were developed for the weedy herb Anacyclus clavatus to study the genetic structure of hybrid zones with closely related taxa in the western Mediterranean Basin, where different floral phenotypes are present. •
  
  
  RESULTS
  We obtained two microsatellite libraries using next-generation sequencing and Sanger sequencing of cloned restriction fragments. A total of 13 polymorphic and 11 monomorphic loci were identified in three Iberian populations of A. clavatus. The primers amplified di- and trinucleotide repeats with 1-8 alleles per locus. Most primers also amplified in A. homogamos, A. monanthos, A. radiatus, and A. valentinus. •
  
  
  CONCLUSIONS
  These results indicate the utility of these markers in A. clavatus for population genetic and hybridization studies as well as their applicability across the genus.
authors.affiliation: Departamento de Biodiversidad y Conservación, Real Jardín Botánico (RJB), Consejo Superior de Investigaciones Científicas (CSIC), Plaza de Murillo 2, 28014 Madrid, Spain.
authors.forename: Alicia
authors.initials: A
authors.lastname: Agudo
authors.affiliation: Departamento de Ecología Integrativa, Estación Biológica de Doñana (EBD), Consejo Superior de Investigaciones Científicas (CSIC), Avenida Américo Vespucio s/n, 41092 Seville, Spain.
authors.forename: F Xavier
authors.initials: FX
authors.lastname: Picó
authors.affiliation: Departamento de Biodiversidad y Conservación, Real Jardín Botánico (RJB), Consejo Superior de Investigaciones Científicas (CSIC), Plaza de Murillo 2, 28014 Madrid, Spain.
authors.forename: Inés
authors.initials: I
authors.lastname: Alvarez
keywords: Anacyclus clavatus; Asteraceae; hybridization; population genetics; weeds