title: Differentiation of human adipose-derived stem cells into neuron-like cells by Radix Angelicae Sinensis.
abstract: Human adipose tissues are an ideal source of stem cells. It is important to find inducers that can safely and effectively differentiate stem cells into functional neurons for clinical use. In this study, we investigate the use of Radix Angelicae Sinensis as an inducer of neuronal differentiation. Primary human adipose-derived stem cells were obtained from adult subcutaneous fatty tissue, then pre-induced with 10% Radix Angelicae Sinensis injection for 24 hours, and incubated in serum-free Dulbecco's modified Eagle's medium/Nutrient Mixture F-12 containing 40% Radix Angelicae Sinensis to induce its differentiation into neuron-like cells. Butylated hydroxyanisole, a common inducer for neuronal differentiation, was used as the control. After human adipose-derived stem cells differentiated into neuron-like cells under the induction of Radix Angelicae Sinensis for 24 hours, the positive expression of neuron-specific enolase was lower than that of the butylated hydroxyanisole-induced group, and the expression of glial fibrillary acidic protein was negative. After they were induced for 48 hours, the positive expression of neuron specific enolase in human adipose-derived stem cells was significantly higher than that of the butylated hydroxyanisole-induced group. Our experimental findings indicate that Radix Angelicae Sinensis can induce human adipose-derived stem cell differentiation into neuron-like cells and produce less cytotoxicity.
authors.affiliation: Department of Histology and Embryology, Luzhou Medical College, Luzhou 646000, Sichuan Province, China.
authors.forename: Qiaozhi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Histology and Embryology, Luzhou Medical College, Luzhou 646000, Sichuan Province, China.
authors.forename: Lile
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Histology and Embryology, Luzhou Medical College, Luzhou 646000, Sichuan Province, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Histology and Embryology, Luzhou Medical College, Luzhou 646000, Sichuan Province, China.
authors.forename: Guangyi
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Electronic Microscopy, Luzhou Medical College, Luzhou 646000, Sichuan Province, China.
authors.forename: Jiyan
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Department of Histology and Embryology, Luzhou Medical College, Luzhou 646000, Sichuan Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yu
keywords: Radix Angelicae Sinensis; adipocytes; cytotoxicity; differentiation; grants-supported paper; human adipose-derived stem cells; neural regeneration; neuron-like cells; neuroregeneration; stem cells