title: Proteomic characterization of the outer membrane vesicle of Pseudomonas putida KT2440.
abstract: Outer membrane vesicles (OMVs) are produced by various pathogenic Gram-negative bacteria such as Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii. In this study, we isolated OMVs from a representative soil bacterium, Pseudomonas putida KT2440, which has a biodegradative activity toward various aromatic compounds. Proteomic analysis identified the outer membrane proteins (OMPs) OprC, OprD, OprE, OprF, OprH, OprG, and OprW as major components of the OMV of P. putida KT2440. The production of OMVs was dependent on the nutrient availability in the culture media, and the up- or down-regulation of specific OMPs was observed according to the culture conditions. In particular, porins (e.g., benzoate-specific porin, BenF-like porin) and enzymes (e.g., catechol 1,2-dioxygenase, benzoate dioxygenase) for benzoate degradation were uniquely found in OMVs prepared from P. putida KT2440 that were cultured in media containing benzoate as the energy source. OMVs of P. putida KT2440 showed low pathological activity toward cultured cells that originated from human lung cells, which suggests their potential as adjuvants or OMV vaccine carriers. Our results suggest that the protein composition of the OMVs of P. putida KT2440 reflects the characteristics of the total proteome of P. putida KT2440.
authors.affiliation: Division of Life Science, Korea Basic Science Institute , Daejeon 305-806, Republic of Korea.
authors.forename: Chi-Won
authors.initials: CW
authors.lastname: Choi
authors.forename: Edmond Changkyun
authors.initials: EC
authors.lastname: Park
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Yun
authors.forename: Sang-Yeop
authors.initials: SY
authors.lastname: Lee
authors.forename: Yeol Gyun
authors.initials: YG
authors.lastname: Lee
authors.forename: Yeonhee
authors.initials: Y
authors.lastname: Hong
authors.forename: Kyeong Ryang
authors.initials: KR
authors.lastname: Park
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Gun-Hwa
authors.initials: GH
authors.lastname: Kim
authors.forename: Seung Il
authors.initials: SI
authors.lastname: Kim
mesh_terms: D017209:Apoptosis; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D002460:Cell Line; D002853:Chromatography, Liquid; D006801:Humans; D046529:Microscopy, Electron, Transmission; D040901:Proteomics; D016958:Pseudomonas putida / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D001425:Bacterial Outer Membrane Proteins
keywords: Pseudomonas putida KT2440; mass spectrometry; outer membrane vesicle