title: Differential ion mobility spectrometry coupled to tandem mass spectrometry enables targeted leukemia antigen detection.
abstract: Differential ion mobility spectrometry (DIMS) can be used as a filter to remove undesired background ions from reaching the mass spectrometer. The ability to use DIMS as a filter for known analytes makes DIMS coupled to tandem mass spectrometry (DIMS-MS/MS) a promising technique for the detection of cancer antigens that can be predicted by computational algorithms. In experiments using DIMS-MS/MS that were performed without the use of high-performance liquid chromatography (HPLC), a predicted model antigen, GLR (FLSSANEHL), was detected at a concentration of 10 pM (20 amol) in a mixture containing 94 competing model peptide antigens, each at a concentration of 1 μM. Without DIMS filtering, the GLR peptide was undetectable in the mixture even at 100 nM. Again, without using HPLC, DIMS-MS/MS was used to detect 2 of 3 previously characterized antigens produced by the leukemia cell line U937.A2. Because of its sensitivity, a targeted DIMS-MS/MS methodology can likely be used to probe for predicted cancer antigens from cancer cell lines as well as human tumor samples.
authors.affiliation: Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill , 450 West Drive, 21-244, Chapel Hill, North Carolina 27599, United States.
authors.forename: Udara
authors.initials: U
authors.lastname: Dharmasiri
authors.forename: Samantha L
authors.initials: SL
authors.lastname: Isenberg
authors.forename: Gary L
authors.initials: GL
authors.lastname: Glish
authors.forename: Paul M
authors.initials: PM
authors.lastname: Armistead
mesh_terms: D000465:Algorithms; D000951:Antigens, Neoplasm / Q000032:analysis*; D045744:Cell Line, Tumor; D006801:Humans; D007938:Leukemia / Q000276:immunology* / Q000473:pathology; D008956:Models, Chemical; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000951:Antigens, Neoplasm
keywords: DIMS; Differential ion mobility spectrometry; FAIMS; cancer antigen; mass spectrometry