title: The impact of ozone treatment on changes in biologically active substances of cardamom seeds.
abstract: The overall objective of this study was to develop a decontamination method against microorganisms in cardamom (Elettaria cardamomum (L.) Maton) seeds using ozone as a decontaminating agent. Ozone treatment was conducted 3 times, at 24-h intervals, and the parameters of the process were determined assuring the least possible losses of biologically active substances (essential oils and polyphenols): ozone concentration 160 to 165.0 g/m(3) ; flow rate 0.1 L/min; pressure 0.5 atm; time 30 min. After each step of decontamination, the microbiological profile of the cardamom seeds was studied, and the contaminating microflora was identified. Next to the microbiological profile, the total polyphenol content (TPC), composition of essential oils, free radical-scavenging capacity, total antioxidant capacity, ferric-reducing antioxidant power (FRAP), and LC-MS polyphenol analysis were determined. This study shows that extract from cardamom seeds after ozone treatment is characterized by a better radical scavenging activity (IC(50) = 24.18 ± 0.04 mg/mL) than the control sample (IC(50) = 31.94 ± 0.05 mg/mL). The extract from cardamom seeds after ozone treatment showed an improved FRAP activity as well (613.64 ± 49.79 mmol TE/g compared to 480.29 ± 30.91 mmol TE/g of control sample). The TPC and the total antioxidant capacity were negatively affected, respectively, 41.2% and 16.2%, compared to the control sample.
authors.affiliation: Inst. of General Food Chemistry, Faculty of Biotechnology and Food Sciences, Lodz Univ. of Technology, Lodz, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Joanna Brodowska
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Śmigielski
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Nowak
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Brodowska
authors.forename: Rik
authors.initials: R
authors.lastname: Catthoor
authors.forename: Agata
authors.initials: A
authors.lastname: Czyżowska
mesh_terms: D028523:Elettaria / Q000737:chemistry* / Q000382:microbiology; D005516:Food Microbiology; D016166:Free Radical Scavengers / Q000737:chemistry; D009822:Oils, Volatile / Q000737:chemistry; D010126:Ozone / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry; D059808:Polyphenols / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry* / Q000382:microbiology
chemical_list: D016166:Free Radical Scavengers; D009822:Oils, Volatile; D010936:Plant Extracts; D010938:Plant Oils; D059808:Polyphenols; D010126:Ozone
keywords: antioxidant activity; cardamom seeds; decontamination; ozone; polyphenols