title: Isolation and characterization of microsatellite markers for Shorea platyclados (Dipterocarpaceae).
abstract: OBJECTIVE
  Microsatellite markers were isolated and characterized in Shorea platyclados (Dipterocarpaceae) for DNA profiling and genetic diversity assessment of this tropical timber species. •
  
  
  RESULTS
  Fifteen polymorphic microsatellite loci were developed and characterized in S. platyclados using a genomic library enriched for dinucleotide (CT) repeats. The primers amplified dinucleotide repeats with 3-14 alleles per locus across four natural populations. The observed and expected heterozygosities ranged from 0.292 to 1.000 and from 0.301 to 0.894, respectively. No significant deviation from Hardy-Weinberg equilibrium was detected in the 15 loci. Four loci pairs displayed linkage disequilibrium. •
  
  
  CONCLUSIONS
  These highly polymorphic markers are adequate for DNA profiling and studies of population genetics in S. platyclados.
authors.affiliation: Genetics Laboratory, Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia.
authors.forename: Chin Hong
authors.initials: CH
authors.lastname: Ng
authors.affiliation: Genetics Laboratory, Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia.
authors.forename: Chai Ting
authors.initials: CT
authors.lastname: Lee
authors.affiliation: Genetics Laboratory, Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia.
authors.forename: Soon Leong
authors.initials: SL
authors.lastname: Lee
authors.affiliation: Genetics Laboratory, Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia.
authors.forename: Lee Hong
authors.initials: LH
authors.lastname: Tnah
authors.affiliation: Genetics Laboratory, Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia.
authors.forename: Kevin Kit Siong
authors.initials: KK
authors.lastname: Ng
keywords: DNA marker; Shorea platyclados.; hill dipterocarp; microsatellite