title: Global view of transcriptome in the brains of aged NR2B transgenic mice.
abstract: NR2B subunits are involved in regulating aging, in particular, age-related learning and memory deficits. We examined 19-month-old NR2B transgenic mice and their littermate controls. First, we detected expression of the NR2B subunit gene, Grin2b, in the neocortex of transgenic mice using real-time PCR. Next, we used microarrays to examine differences in neocortical gene expression. Pathway and signal-net analyses identified multiple pathways altered in the transgenic mice, including the P53, Jak-STAT, Wnt, and Notch pathways, as well as regulation of the actin cytoskeleton and neuroactive ligand-receptor interactions. Further signal-net analysis highlighted the P53 and insulin-like growth factor pathways as key regulatory pathways. Our results provide new insight into understanding the molecular mechanisms of NR2B regulated age-related memory storage, normal organismal aging and age-related disease.
authors.affiliation: Key Lab of Brain Functional Genomics, MOE&STCSM, Institute of Cognitive Neuroscience, East China Normal University, Shanghai 200062, China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Lab of Brain Functional Genomics, MOE&STCSM, Institute of Cognitive Neuroscience, East China Normal University, Shanghai 200062, China.
authors.forename: Men
authors.initials: M
authors.lastname: Su
authors.affiliation: Key Lab of Brain Functional Genomics, MOE&STCSM, Institute of Cognitive Neuroscience, East China Normal University, Shanghai 200062, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Lab of Brain Functional Genomics, MOE&STCSM, Institute of Cognitive Neuroscience, East China Normal University, Shanghai 200062, China ; Shanghai Engineering Research Center for Molecular Therapeutics and New Drug Development, East China Normal University, Shanghai 200062, China.
authors.forename: Yinghe
authors.initials: Y
authors.lastname: Hu
keywords: NR2B transgenic mice; P53; aging; gene expression; grants-supported paper; insulin-like growth factor; memory; neural regeneration; neuroregeneration