title: Isolation and characterization of nine polymorphic microsatellite loci in Piper solmsianum (piperaceae).
abstract: OBJECTIVE
  Nine microsatellite (simple sequence repeat [SSR]) loci were characterized for natural populations of Piper solmsianum, a potential source of bioactive secondary metabolites, and analyzed to assess the levels of genetic diversity in this species. •
  
  
  RESULTS
  Based on an enriched library using the oligonucleotides (CT)8 and (GT)8, a total of 19 pairs of SSR primers were designed and nine of them were highly polymorphic after screening of 37 specimens from two populations. The number of alleles per locus ranged from one to six while the observed heterozygosity for polymorphic loci ranged from 0.000 to 0.875. •
  
  
  CONCLUSIONS
  The SSR regions characterized were informative, and the genetic markers will be useful to assess the genetic diversity and gene flow in populations of P. solmsianum.
authors.affiliation: Núcleo de Apoio à Pesquisas em Diversidade Molecular de Produtos Naturais (NAP-PN), Instituto de Química, Universidade de São Paulo, C.P. 26077, 05508-000 São Paulo, São Paulo, Brazil.
authors.forename: Nídia C
authors.initials: NC
authors.lastname: Yoshida
authors.affiliation: Centro de Pesquisa e Desenvolvimento de Recursos Genéticos Vegetais, Instituto Agronômico de Campinas (IAC), C.P. 28, 13075-630 Campinas, São Paulo, Brazil.
authors.forename: Paula F
authors.initials: PF
authors.lastname: Lima
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 13418-900 Piracicaba, São Paulo, Brazil.
authors.forename: Regina H G
authors.initials: RH
authors.lastname: Priolli
authors.affiliation: Núcleo de Apoio à Pesquisas em Diversidade Molecular de Produtos Naturais (NAP-PN), Instituto de Química, Universidade de São Paulo, C.P. 26077, 05508-000 São Paulo, São Paulo, Brazil.
authors.forename: Massuo J
authors.initials: MJ
authors.lastname: Kato
authors.affiliation: Centro de Pesquisa e Desenvolvimento de Recursos Genéticos Vegetais, Instituto Agronômico de Campinas (IAC), C.P. 28, 13075-630 Campinas, São Paulo, Brazil.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Colombo
keywords: Piper solmsianum; Piperaceae; allelic polymorphism; genetic diversity; medicinal plant; simple sequence repeat (SSR)