title: Backbone assignments of the apo and Zn(II) protoporphyrin IX-bound states of the soluble form of rat heme oxygenase-1.
abstract: In nature, heme is a prosthetic group that is universally used as a cofactor for heme proteins. It is necessary for the execution of fundamental biological processes including electron transfer, oxidation and metabolism. However, free heme is toxic to cells, because of its capability to enhance oxidative stress, hence its cellular concentration is strictly regulated through multiple mechanisms. Heme oxygenase (HO) serves as an irreplaceable member in the heme degradation system. It is a ubiquitous protein, existing in many species including mammals, higher plants, and interestingly, certain pathogenic bacteria. In the HO reaction, HO catalyzes oxidative cleavage of heme to generate biliverdin and release carbon monoxide and ferrous iron. Because of the beneficial effects of these heme catabolism products, HO plays a key role in iron homeostasis and in defense mechanism against oxidative stress. HO is composed of an N-terminal structured region and a C-terminal membrane-bound region. Furthermore, the soluble form of HO, which is obtainable by excision of the membrane-bound region, retains its catalytic activity. Here, we present the backbone resonance assignments of the soluble form (residues 1-232) of HO-1 in the free and Zn(II) protoporphyrin IX (ZnPP)-bound states, and analyzed the structural differences between the states. ZnPP is a potent enzyme inhibitor, and the ZnPP-bound structure of HO-1 mimics the heme-bound structure. These assignments provide the structural basis for a detailed investigation of the HO-1 function.
authors.affiliation: Bioorganic Research Institute, Suntory Foundation for Life Sciences, 1-1-1 Wakayamadai, Shimamoto, Mishima, Osaka, 618-8503, Japan.
authors.forename: Erisa
authors.initials: E
authors.lastname: Harada
authors.forename: Masakazu
authors.initials: M
authors.lastname: Sugishima
authors.forename: Jiro
authors.initials: J
authors.lastname: Harada
authors.forename: Masato
authors.initials: M
authors.lastname: Noguchi
authors.forename: Keiichi
authors.initials: K
authors.lastname: Fukuyama
authors.forename: Kenji
authors.initials: K
authors.lastname: Sugase
mesh_terms: D000818:Animals; D001051:Apoenzymes / Q000737:chemistry* / Q000378:metabolism*; D051547:Heme Oxygenase-1 / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D011524:Protoporphyrins / Q000378:metabolism*; D051381:Rats
chemical_list: D001051:Apoenzymes; D011524:Protoporphyrins; C017803:zinc protoporphyrin; D051547:Heme Oxygenase-1