title: Acupoint injection of autologous stromal vascular fraction and allogeneic adipose-derived stem cells to treat hip dysplasia in dogs.
abstract: Stem cells isolated from adipose tissue show great therapeutic potential in veterinary medicine, but some points such as the use of fresh or cultured cells and route of administration need better knowledge. This study aimed to evaluate the effect of autologous stromal vascular fraction (SVF, n = 4) or allogeneic cultured adipose-derived stem cells (ASCs, n = 5) injected into acupuncture points in dogs with hip dysplasia and weak response to drug therapy. Canine ASCs have proliferation and differentiation potential similar to ASCs from other species. After the first week of treatment, clinical evaluation showed marked improvement compared with baseline results in all patients treated with autologous SVF and three of the dogs treated with allogeneic ASCs. On days 15 and 30, all dogs showed improvement in range of motion, lameness at trot, and pain on manipulation of the joints, except for one ASC-treated patient. Positive results were more clearly seen in the SVF-treated group. These results show that autologous SVF or allogeneic ASCs can be safely used in acupoint injection for treating hip dysplasia in dogs and represent an important therapeutic alternative for this type of pathology. Further studies are necessary to assess a possible advantage of SVF cells in treating joint diseases.
authors.affiliation: Laboratory of Stem Cells and Tissue Engineering, Universidade Luterana do Brasil, 92425-900 Canoas, RS, Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: Marx
authors.affiliation: Laboratory of Stem Cells and Tissue Engineering, Universidade Luterana do Brasil, 92425-900 Canoas, RS, Brazil.
authors.forename: Maiele Dornelles
authors.initials: MD
authors.lastname: Silveira
authors.affiliation: Clinivetis Clinica Veterinaria, 93310-500 Novo Hamburgo, RS, Brazil.
authors.forename: Isabel
authors.initials: I
authors.lastname: Selbach
authors.affiliation: Laboratory of Stem Cells and Tissue Engineering, Universidade Luterana do Brasil, 92425-900 Canoas, RS, Brazil.
authors.forename: Ariel Silveira
authors.initials: AS
authors.lastname: da Silva
authors.affiliation: Center for Experimental Biological Models, Pontificia Universidade Catolica do Rio Grande do Sul, 90619-900 Porto Alegre, RS, Brazil.
authors.forename: Luisa Maria Gomes de Macedo
authors.initials: LM
authors.lastname: Braga
authors.affiliation: Laboratory of Stem Cells and Tissue Engineering, Universidade Luterana do Brasil, 92425-900 Canoas, RS, Brazil.
authors.forename: Melissa
authors.initials: M
authors.lastname: Camassola
authors.affiliation: Laboratory of Stem Cells and Tissue Engineering, Universidade Luterana do Brasil, 92425-900 Canoas, RS, Brazil.
authors.forename: Nance Beyer
authors.initials: NB
authors.lastname: Nardi