title: Effect of hyperbaric hepatic hyperoxia on the liver of rats submitted to intermittent ischemia/reperfusion injury.
abstract: OBJECTIVE
  To determine the effect of hyperbaric hyperoxia as hepatic preconditioning on hepatocellular integrity in rats submitted to intermittent hepatic ischemia/reperfusion injury.
  
  
  METHODS
  Twenty male Wistar rats were divided into 4 groups (SHAM, I/R, HBO-I/R and CONTROL). The surgical technique consisted of total clamping of the hepatic pedicle for 15 min, followed by reperfusion for 5 min, performed twice. The application of hyperbaric oxygen (HBO) was carried out in a collective chamber (simultaneous exposure of 4 rats) pressurized directly with oxygen at 2 ATA for 60 min. Tissue malondialdehyde (MDA) levels were determined and blood samples were collected for the determination of serum AST and ALT levels. Data were analyzed statistically by the Mann-Whitney test, with the level of significance set at p < 0.05.
  
  
  RESULTS
  A statistically significant difference in MDA (p< 0.05) was observed between control and HBO-I/R, but not between control and I/R. Regarding AST, there was a difference between control and I/R and HBO-I/R. Analysis of ALT revealed a significant difference between control and I/R (p<0.05) and between I/R and HBO-I/R, with no difference between control and HBO-IR.
  
  
  CONCLUSIONS
  Hyperoxic preconditioning proved to be favorable regarding alanine transaminase, but not aspartate aminotranserase or malondialdehyde levels.
authors.affiliation: Faculty of Medicine of Marília, Marília, São Paulo, Brasil.
authors.forename: Diego Elias da Silva
authors.initials: DE
authors.lastname: Caldeira
authors.affiliation: Ribeirão Preto Medical School, Ribeirão Preto, São Paulo, Brasil.
authors.forename: Marina Rodrigues Garcia da
authors.initials: MR
authors.lastname: Silveira
authors.affiliation: Ribeirão Preto Medical School, Ribeirão Preto, São Paulo, Brasil.
authors.forename: Maria Rita Rodrigues Alves
authors.initials: MR
authors.lastname: Margarido
authors.affiliation: Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of São Paulo.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Vanni
authors.affiliation: Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of São Paulo.
authors.forename: Omar
authors.initials: O
authors.lastname: Feres
authors.affiliation: Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of São Paulo.
authors.forename: Orlando Castro E
authors.initials: OC
authors.lastname: Silva
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D006931:Hyperbaric Oxygenation / Q000379:methods*; D019194:Ischemic Preconditioning / Q000379:methods*; D008099:Liver / Q000098:blood supply*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008930:Mitochondria, Liver / Q000502:physiology; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000628:therapy*; D015203:Reproducibility of Results; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D008315:Malondialdehyde; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase