title: Cross-species comparison of the Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia mallei quorum-sensing regulons.
abstract: Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia mallei (the Bptm group) are close relatives with very different lifestyles: B. pseudomallei is an opportunistic pathogen, B. thailandensis is a nonpathogenic saprophyte, and B. mallei is a host-restricted pathogen. The acyl-homoserine lactone quorum-sensing (QS) systems of these three species show a high level of conservation. We used transcriptome sequencing (RNA-seq) to define the quorum-sensing regulon in each species, and we performed a cross-species analysis of the QS-controlled orthologs. Our analysis revealed a core set of QS-regulated genes in all three species, as well as QS-controlled factors shared by only two species or unique to a given species. This global survey of the QS regulons of B. pseudomallei, B. thailandensis, and B. mallei serves as a platform for predicting which QS-controlled processes might be important in different bacterial niches and contribute to the pathogenesis of B. pseudomallei and B. mallei.
authors.affiliation: Department of Microbiology, University of Washington School of Medicine, Seattle, Washington, USA.
authors.forename: Charlotte D
authors.initials: CD
authors.lastname: Majerczyk
authors.affiliation: Department of Microbiology, University of Washington School of Medicine, Seattle, Washington, USA.
authors.forename: Mitchell J
authors.initials: MJ
authors.lastname: Brittnacher
authors.affiliation: Department of Microbiology, University of Washington School of Medicine, Seattle, Washington, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Jacobs
authors.affiliation: NuGen, San Carlos, California, USA.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Armour
authors.affiliation: Department of Microbiology, University of Washington School of Medicine, Seattle, Washington, USA.
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Radey
authors.affiliation: Department of Chemistry and Biochemistry, Middlebury College, Middlebury, Vermont, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Bunt
authors.affiliation: Department of Microbiology, University of Washington School of Medicine, Seattle, Washington, USA.
authors.forename: Hillary S
authors.initials: HS
authors.lastname: Hayden
authors.affiliation: Department of Microbiology, University of Washington School of Medicine, Seattle, Washington, USA.
authors.forename: Ryland
authors.initials: R
authors.lastname: Bydalek
authors.affiliation: Department of Microbiology, University of Washington School of Medicine, Seattle, Washington, USA epgreen@uw.edu.
authors.forename: E Peter
authors.initials: EP
authors.lastname: Greenberg
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D019117:Burkholderia / Q000145:classification / Q000235:genetics* / Q000502:physiology*; D042726:Burkholderia mallei / Q000145:classification / Q000235:genetics / Q000502:physiology; D016957:Burkholderia pseudomallei / Q000145:classification / Q000235:genetics / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D053038:Quorum Sensing / Q000502:physiology*; D018085:Regulon / Q000502:physiology*; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins