title: First identification of polymorphic microsatellite markers in the Burgundy truffle, Tuber aestivum (Tuberaceae).
abstract: OBJECTIVE
  Tuber aestivum, the most common truffle in Europe, plays an important role in the commercial truffle market. For the first time, microsatellite primers were developed to investigate polymorphism within this species. •
  
  
  RESULTS
  Using direct shotgun pyrosequencing, 15 polymorphic microsatellites were identified out of the 7784 perfect microsatellites present in the 534620 reads obtained. Tested on 75 samples, these microsatellites were highly polymorphic. The number of alleles varied from four to 15, and the expected heterozygosity ranged from 0.266 to 0.620. A multilocus analysis allowed the identification of 63 genotypes over the 75 samples analyzed. •
  
  
  CONCLUSIONS
  Direct shotgun pyrosequencing is a fast and relatively low-cost technique allowing identification of microsatellites in nonmodel species. The microsatellites developed in this study will be useful in population genetic studies to infer the evolutionary history of this species.
authors.affiliation: UMR Agroécologie INRA 1347/AgroSup/Université de Bourgogne, Pôle Interactions Plantes Microorganismes ERL 6300 CNRS, BP 86510, 21065 Dijon CEDEX, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Molinier
authors.affiliation: UMR "Interaction Arbres Microorganismes", Centre INRA Nancy, 54280 Champenoux, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Murat
authors.affiliation: UMR "Interaction Arbres Microorganismes", Centre INRA Nancy, 54280 Champenoux, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Morin
authors.affiliation: Inoplant, 13 rue des Souhaits, 21110 Aiserey, France.
authors.forename: Armelle
authors.initials: A
authors.lastname: Gollotte
authors.affiliation: UMR Agroécologie INRA 1347/AgroSup/Université de Bourgogne, Pôle Interactions Plantes Microorganismes ERL 6300 CNRS, BP 86510, 21065 Dijon CEDEX, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Wipf
authors.affiliation: UMR "Interaction Arbres Microorganismes", Centre INRA Nancy, 54280 Champenoux, France.
authors.forename: Francis
authors.initials: F
authors.lastname: Martin
keywords: Tuber aestivum; Tuberaceae; direct shotgun pyrosequencing; polymorphism; truffle