title: A novel high-resolution melting analysis-based method for Yersinia enterocolitica genotyping.
abstract: Pathogenic Yersinia enterocolitica strains are associated with biotypes 1B, 2-5, while environmental strains with biotype 1A. In this work a method for Y. enterocolitica genotyping based on HRMA to determine SNPs was developed and the genetic diversity of 50 strains was determined. The strains were clustered into three groups consistent with the pathogenic profile of each biotype. The results provided a better understanding of the Y. enterocolitica genetic variability.
authors.affiliation: Brazilian Reference Center on Yersinia spp. other than Y. pestis, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Brazil.
authors.forename: Roberto A
authors.initials: RA
authors.lastname: Souza
authors.affiliation: Brazilian Reference Center on Yersinia spp. other than Y. pestis, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Brazil. Electronic address: jufalcao@fcfrp.usp.br.
authors.forename: Juliana P
authors.initials: JP
authors.lastname: Falcão
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*; D044366:Transition Temperature; D015008:Yersinia enterocolitica / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial
keywords: Genetic variability; High resolution melting analysis (HRMA); Molecular epidemiology; Single-nucleotide polymorphisms (SNPs); Yersinia enterocolitica