title: Microsatellite primers for the Pacific Northwest conifer Callitropsis nootkatensis (Cupressaceae).
abstract: OBJECTIVE
  Microsatellite primers were developed for Nootka cypress (Callitropsis nootkatensis) to provide quantitative measures for gene conservation that can assist in guiding management decisions for a species experiencing climate-induced decline. •
  
  
  RESULTS
  Using multiplexed massively parallel sequencing, we identified 136,785 microsatellite-containing sequences from 489,625 Illumina paired-end 80-bp reads. After stringent filtering, we selected 144 primer pairs and screened variation at these loci in five populations of C. nootkatensis. Loci show between three and 36 dinucleotide repeats per locus, with an average of 13. Screening of these markers in the Pacific Northwest relative Chamaecyparis lawsoniana demonstrated no marker transferability. This finding highlights the narrow taxonomic utility of microsatellite markers in Callitropsis. •
  
  
  CONCLUSIONS
  These microsatellites show high polymorphism and can be used for routine screening of natural variation in Callitropsis nootkatensis, and will be particularly helpful in identifying clones and inbred relatives at the stand-level.
authors.affiliation: Pacific Northwest Research Station, USDA Forest Service, 3200 SW Jefferson Way, Corvallis, Oregon 97331 USA.
authors.forename: Tara N
authors.initials: TN
authors.lastname: Jennings
authors.affiliation: Pacific Northwest Research Station, USDA Forest Service, 3200 SW Jefferson Way, Corvallis, Oregon 97331 USA ; Horticultural Crops Research Unit, USDA Agricultural Research Service, 3240 NW Orchard Avenue, Oregon 97331 USA.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Knaus
authors.affiliation: Pacific Northwest Research Station, USDA Forest Service, 3200 SW Jefferson Way, Corvallis, Oregon 97331 USA.
authors.forename: Katherine
authors.initials: K
authors.lastname: Alderman
authors.affiliation: Pacific Northwest Research Station, USDA Forest Service, 11305 Glacier Highway, Juneau, Alaska 99801 USA.
authors.forename: Paul E
authors.initials: PE
authors.lastname: Hennon
authors.affiliation: Pacific Northwest Research Station, USDA Forest Service, 11305 Glacier Highway, Juneau, Alaska 99801 USA.
authors.forename: David V
authors.initials: DV
authors.lastname: D'Amore
authors.affiliation: Pacific Northwest Research Station, USDA Forest Service, 3200 SW Jefferson Way, Corvallis, Oregon 97331 USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Cronn
keywords: Callitropsis nootkatensis; Chamaecyparis nootkatensis; Nootka cypress; Pacific Northwest; climate change; germplasm