title: Effect of minocycline on cerebral ischemia-reperfusion injury.
abstract: Minocylcine, a tetracycline derivate, has been shown to cross the blood-brain barrier and enter the central nervous system. In this study, cerebral ischemia-reperfusion injury models were established using the suture method, and minocycline was immediately injected intraperitoneally after cerebral ischemia-reperfusion (22.5 mg/kg, initially 45 mg/kg) at a 12-hour interval. Results showed that after minocycline treatment, the volume of cerebral infarction was significantly reduced, the number of surviving cell in the hippocampal CA1 region increased, the number of apoptotic cells decreased, the expression of caspase-3 and poly(adenosine diphosphate-ribose) polymerase-1 protein was down-regulated, and the escape latency in the water maze test was significantly shortened compared with the ischemia-reperfusion group. Our experimental findings indicate that minocycline can protect against neuronal injury induced by focal ischemia-reperfusion, which may be mediated by the inhibition of caspase-3 and poly(adenosine diphosphate-ribose) polymerase-1 protein expression.
authors.affiliation: Department of Physiology, Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, Guangdong Province, China ; Department of Pathology, Mindong Hospital of Fujian Medical University, Fuan 355000, Fujian Province, China.
authors.forename: Yuanyin
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Physiology, Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, Guangdong Province, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Pathology, Guangdong Medical College, Dongguan 523808, Guangdong Province, China.
authors.forename: Jinbao
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of Physiology, Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, Guangdong Province, China.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Department of Physiology, Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, Guangdong Province, China.
authors.forename: Hongling
authors.initials: H
authors.lastname: Fan
authors.affiliation: Department of Physiology, Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, Guangdong Province, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Physiology, Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, Guangdong Province, China.
authors.forename: Quanzhong
authors.initials: Q
authors.lastname: Chang
keywords: apoptosis; brain injury; caspase-3; cerebral ischemia-reperfusion; grants-supported paper; hippocampus; minocycline; neural regeneration; neuroregeneration; poly (adenosine diphosphate-ribose) polymerase-1