title: A non-invasive, rapid method to genotype late-onset Alzheimer's disease-related apolipoprotein E gene polymorphisms.
abstract: The apolipoprotein E gene ε4 allele is considered a negative factor for neural regeneration in late-onset Alzheimer's disease cases. The aim of this study was to establish a non-invasive, rapid method to genotype apolipoprotein E gene polymorphisms. Genomic DNA from mouth swab specimens was extracted using magnetic nanoparticles, and genotyping was performed by real-time PCR using TaqMan-BHQ probes. Genotyping accuracy was validated by DNA sequencing. Our results demonstrate 100% correlation to DNA sequencing, indicating reliability of our protocol. Thus, the method we have developed for apolipoprotein E genotyping is accurate and reliable, and also suitable for genotyping large samples, which may help determine the role of the apolipoprotein E ε4 allele in neural regeneration in late-onset Alzheimer's disease cases.
authors.affiliation: Department of Neurology, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yi
authors.affiliation: Department of Neurology, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wu
authors.affiliation: Department of Neurology, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China.
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Luo
authors.affiliation: Department of Radiology, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Neurology, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
keywords: DNA sequencing; allele; apolipoprotein E gene; late-onset Alzheimer's disease; nerve regeneration; neural regeneration; neurodegeneration; real-time PCR; risk factor