title: Regulation of adenosine triphosphate-sensitive potassium channels suppresses the toxic effects of amyloid-beta peptide (25-35).
abstract: In this study, we treated PC12 cells with 0-20 μM amyloid-β peptide (25-35) for 24 hours to induce cytotoxicity, and found that 5-20 μM amyloid-β peptide (25-35) decreased PC12 cell viability, but adenosine triphosphate-sensitive potassium channel activator diazoxide suppressed the decrease in PC12 cell viability induced by amyloid-β peptide (25-35). Diazoxide protected PC12 cells against amyloid-β peptide (25-35)-induced increases in mitochondrial membrane potential and intracellular reactive oxygen species levels. These protective effects were reversed by the selective mitochondrial adenosine triphosphate-sensitive potassium channel blocker 5-hydroxydecanoate. An inducible nitric oxide synthase inhibitor, Nω-nitro-L-arginine, also protected PC12 cells from amyloid-β peptide (25-35)-induced increases in both mitochondrial membrane potential and intracellular reactive oxygen species levels. However, the H2O2-degrading enzyme catalase could not reverse the amyloid-β peptide (25-35)-induced increase in intracellular reactive oxygen species. A 24-hour exposure to amyloid-β peptide (25-35) did not result in apoptosis or necrosis, suggesting that the increases in both mitochondrial membrane potential and reactive oxygen species levels preceded cell death. The data suggest that amyloid-β peptide (25-35) cytotoxicity is associated with adenosine triphosphate-sensitive potassium channels and nitric oxide. Regulation of adenosine triphosphate-sensitive potassium channels suppresses PC12 cell cytotoxicity induced by amyloid-β peptide (25-35).
authors.affiliation: Department of Neurology, Yantaishan Hospital, Yantai 264000, Shandong Province, China.
authors.forename: Min
authors.initials: M
authors.lastname: Kong
authors.affiliation: Department of Neurology, Yuhuangding Hospital Affiliated to Qingdao Medical University, Yantai 264000, Shandong Province, China.
authors.forename: Maowen
authors.initials: M
authors.lastname: Ba
authors.affiliation: Department of Neurology, Yantaishan Hospital, Yantai 264000, Shandong Province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liang
authors.affiliation: Department of Neurology, Yantaishan Hospital, Yantai 264000, Shandong Province, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Shao
authors.affiliation: Department of Neurology, Yantaishan Hospital, Yantai 264000, Shandong Province, China.
authors.forename: Tianxia
authors.initials: T
authors.lastname: Yu
authors.affiliation: Department of Neurology, Yantaishan Hospital, Yantai 264000, Shandong Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
keywords: PC12 cell; adenosine triphosphate-sensitive potassium channel; amyloid-β peptide (25–35); grant-supported paper; inducible nitric oxide synthase; mitochondrial membrane potential; neural regeneration; neurodegenerative diseases; neuroregeneration; photographs-containing paper; reactive oxygen species