title: MiR-212 exerts suppressive effect on SKOV3 ovarian cancer cells through targeting HBEGF.
abstract: MicroRNAs (miRNAs) play critical roles in the development and progression of ovarian cancer. We found that miR-212 was significantly downregulated in serum and tissues from epithelial ovarian cancer (EOC) patients. Overexpression of miR-212 in ovarian cancer cells inhibited cell proliferation, migration, and invasion. Luciferase reporter assay confirmed HBEGF as a direct target of miR-212. Overexpression of miR-212 decreased HBEGF expression at both the protein and messenger RNA (mRNA) levels. Knockdown of HBEGF expression in SKOV3 cell line significantly inhibited cell growth, migration, and invasion. HBEGF mRNA level was upregulated in EOC tissues and inversely correlated with miR-212 expression in tissues. Upregulation of HBEGF could attenuate the effect induced by miR-212. These findings indicate that miR-212 displays a tumor-suppressive effect in human ovarian cancer. And miR-212 suppresses cell proliferation, migration, and invasion by targeting the HBEGF transcript, highlighting the therapeutic potential of miR-212 and HBEGF in epithelial ovarian cancer treatment.
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe Road, Zhengzhou, 450052, Henan, China.
authors.forename: Li-Qiang
authors.initials: LQ
authors.lastname: Wei
authors.forename: Hui-Tao
authors.initials: HT
authors.lastname: Liang
authors.forename: Dong-Chun
authors.initials: DC
authors.lastname: Qin
authors.forename: Hui-Fang
authors.initials: HF
authors.lastname: Jin
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Ming-Cong
authors.initials: MC
authors.lastname: She
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D066257:Heparin-binding EGF-like Growth Factor / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D034622:RNA Interference; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics
chemical_list: C587883:HBEGF protein, human; D066257:Heparin-binding EGF-like Growth Factor; C551411:MIRN212 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger