title: Development and characterization of 10 microsatellite markers in Sagina nodosa (Caryophyllaceae).
abstract: OBJECTIVE
  We developed 10 novel microsatellite loci for Sagina nodosa, a diploid perennial arctic-alpine herb. To our knowledge, these are the first microsatellite loci for a Sagina species. •
  
  
  RESULTS
  We performed a low-coverage 454 next-generation sequencing of enriched genomic fragments derived from one individual to generate a massive library of contigs containing potential polymorphic microsatellites. We present data for 10 novel polymorphic microsatellite loci containing di-, tri-, tetra-, and hexanucleotide repeats with two to nine alleles per locus assessed in 29 individuals. •
  
  
  CONCLUSIONS
  These polymorphic microsatellite loci in S. nodosa will provide insights on the population structure and life history of S. nodosa in Isle Royale and other North American populations.
authors.affiliation: Department of Biology, Williams College, Williamstown, Massachusetts 01267 USA ; Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, United Kingdom.
authors.forename: Tarjinder
authors.initials: T
authors.lastname: Singh
authors.affiliation: Department of Biology, Williams College, Williamstown, Massachusetts 01267 USA.
authors.forename: Joan
authors.initials: J
authors.lastname: Edwards
authors.affiliation: Department of Biology, Williams College, Williamstown, Massachusetts 01267 USA.
authors.forename: Luana S
authors.initials: LS
authors.lastname: Maroja
keywords: 454 sequencing; Sagina nodosa; arctic-alpine plant; knotted pearlwort; polymorphism; population genetics