title: Primers for low-copy nuclear genes in the Hawaiian endemic Clermontia (Campanulaceae) and cross-amplification in Lobelioideae.
abstract: OBJECTIVE
  Primers were developed to amplify 12 intron-less, low-copy nuclear genes in the Hawaiian genus Clermontia (Campanulaceae), a suspected tetraploid. •
  
  
  RESULTS
  Data from a pooled 454 titanium run of the partial transcriptomes of seven Clermontia species were used to identify the loci of interest. Most loci were amplified and sequenced directly with success in a representative selection of lobeliads even though several of these loci turned out to be duplicated. Levels of variation were comparable to those observed in commonly used plastid and ribosomal markers. •
  
  
  CONCLUSIONS
  We found evidence of a genome duplication that likely predates the diversification of the Hawaiian lobeliads. Some genes nevertheless appear to be single-copy and should be useful for phylogenetic studies of Clermontia or the entire Lobelioideae subfamily.
authors.affiliation: Tropical Conservation Biology and Environmental Science Program, University of Hawai'i at Hilo, 200 West Kawili Street, Hilo, Hawaii 96720 USA.
authors.forename: Yohan
authors.initials: Y
authors.lastname: Pillon
authors.affiliation: Tropical Conservation Biology and Environmental Science Program, University of Hawai'i at Hilo, 200 West Kawili Street, Hilo, Hawaii 96720 USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Johansen
authors.affiliation: Tropical Conservation Biology and Environmental Science Program, University of Hawai'i at Hilo, 200 West Kawili Street, Hilo, Hawaii 96720 USA.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Sakishima
authors.affiliation: Department of Biology, University of Florida, Gainesville, Florida 32611 USA ; Genetics Institute, University of Florida, Gainesville, Florida 32610 USA.
authors.forename: Srikar
authors.initials: S
authors.lastname: Chamala
authors.affiliation: Department of Biology, University of Florida, Gainesville, Florida 32611 USA ; Genetics Institute, University of Florida, Gainesville, Florida 32610 USA.
authors.forename: W Brad
authors.initials: WB
authors.lastname: Barbazuk
authors.affiliation: Tropical Conservation Biology and Environmental Science Program, University of Hawai'i at Hilo, 200 West Kawili Street, Hilo, Hawaii 96720 USA.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Stacy
keywords: Clermontia; Hawai‘i; Lobelioideae; low-copy nuclear genes; next-generation sequencing; polyploidy