title: Accumulation of phenylpropanoids and correlated gene expression in hairy roots of tartary buckwheat under light and dark conditions.
abstract: Differential expression patterns of flavonoid biosynthetic pathway genes in the hairy roots of tartary buckwheat cultivars "Hokkai T8" and "Hokkai T10" were studied over a time course of the light-dark cycle. The Agrobacterium rhizogenes-mediated transformation system was applied for inducing hairy roots. Further, a total of six phenolic compounds and two anthocyanins were analyzed in the hairy roots which were exposed to both light and dark conditions, and their amounts were estimated by HPLC. The gene expression levels peaked on day 5 of culture during the time course of both dark and light conditions. Notably, FtPAL, Ft4CL, FtC4H, FtCHI, FtF3H, FtF3'H-1, and FtFLS-1 were more highly expressed in Hokkai T10 than in Hokkai T8 under dark conditions, among which FtPAL and FtCHI were found to be significantly upregulated, except on day 20 of culture. Significantly higher levels of phenolic compound, rutin, along with two anthocyanins were detected in the hairy roots of Hokkai T10 under both conditions. Furthermore, among all the phenolic compounds detected, the amount of rutin in Hokkai T10 hairy roots was found to be ∼5-fold (59,01 mg/g dry weight) higher than that in the control (12.45 mg/g dry weight) at the respective time periods under light and dark conditions.
authors.affiliation: Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, 305-764, Korea, thwe.ayeaye@gmail.com.
authors.forename: Aye Aye
authors.initials: AA
authors.lastname: Thwe
authors.forename: YeJi
authors.initials: Y
authors.lastname: Kim
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.forename: Yeon Bok
authors.initials: YB
authors.lastname: Kim
authors.forename: Nam-Il
authors.initials: NI
authors.lastname: Park
authors.forename: Haeng Hoon
authors.initials: HH
authors.lastname: Kim
authors.forename: Sun-Ju
authors.initials: SJ
authors.lastname: Kim
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Park
mesh_terms: D060054:Agrobacterium / Q000235:genetics; D003624:Darkness; D019612:Fagopyrum / Q000235:genetics / Q000378:metabolism*; D005419:Flavonoids / Q000096:biosynthesis*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D008027:Light; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*
chemical_list: D005419:Flavonoids; D010940:Plant Proteins