title: Immunosensor based on electrodeposition of gold-nanoparticles and ionic liquid composite for detection of Salmonella pullorum.
abstract: In order to increase the reproducibility and stability of electrochemical immunosensor, which is a key issue for its application and popularization, an accurate and stable immunosensor for rapid detection of Salmonella pullorum (S. pullorum) was proposed in this study. The immunosensor was fabricated by modifying Screen-printed Carbon Electrode (SPCE) with electrodeposited gold nanoparticles (AuNPs), HRP-labeled anti-S. pullorum and ionic liquids (ILs) (AuNP/HRP/IL). AuNPs are electrodeposited on the working electrode surface to increase the amount of antibodies that bind to the electrode and then modified with ILs to protect the antibodies from being inactivated in the test environment and maintain their biological activity and the stability of the detection electrode. The electrochemical characteristics of the stepwise modified electrodes and the detection of S. pullorum were investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). As shown in the results of the experiments, AuNPs with unique electrochemical properties as well as biocompatibility characteristics have been proven to be able to strengthen the antibody combination effectively and to increase the electrochemical response signal. In addition, a crucial assessment regarding implementation of stability and reproducibility analysis of a range of immunosensors is provided. We found that application of AuNPs/ILs in the immune modified electrodes showed obvious improvement when compared with other groups. Given their high levels of reproducibility, stability, target specificity and sensitivity, AuNPs and ILs were considered to be excellent elements for electrode modification.
authors.affiliation: Food Safety Key Lab of Zhejiang Province, College of Food Science and Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou 310035, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Food Safety Key Lab of Zhejiang Province, College of Food Science and Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou 310035, PR China.
authors.forename: Wenchao
authors.initials: W
authors.lastname: Dou
authors.affiliation: Food Safety Key Lab of Zhejiang Province, College of Food Science and Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou 310035, PR China. Electronic address: zhaogy-user@163.com.
authors.forename: Guangying
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Food Safety Key Lab of Zhejiang Province, College of Food Science and Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou 310035, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D004595:Electroplating; D006046:Gold / Q000032:analysis*; D052578:Ionic Liquids; D053758:Nanoparticles / Q000032:analysis*; D015203:Reproducibility of Results; D012475:Salmonella / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D052578:Ionic Liquids; D006046:Gold
keywords: Accuracy and stability; AuNPs; ILs; Immunosensor; S. pullorum