title: Analysis of telomerase activity based on a spired DNA tetrahedron TS primer.
abstract: The development of sensitive telomerase biosensors is hindered by the restricted accessibility of telomere strand (TS) primer and the limited enzyme reaction space, which is mainly confined by the vertical distance. In this work, we designed an electrochemical telomerase biosensor based on a spired DNA tetrahedron TS primer (STTS). By adding a rigid dsDNA spire onto the top of the DNA tetrahedron, we successfully regulated the distance between the TS primer and the surface, and thus greatly facilitated the telomerase elongation on surface. The signal-to-noise ratio was 2 times higher than TSP without the spire structure. The limit of detection was calculated to be lower than 10 HeLa cells, which is at least 2 magnitudes lower than other surface extension-based electrochemical telomerase sensors without amplification. The practicability of STTS sensor was also demonstrated by analysing various other cell lines including cancer cells, stem cells of high telomerase activity and somatic cells of low telomerase activity.
authors.affiliation: Laboratory of Biometrology, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Laboratory of Biometrology, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, P.R. China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Department of Chemistry, College of Life and Environmental Sciences, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, P.R. China.
authors.forename: Lele
authors.initials: L
authors.lastname: Wang
authors.affiliation: Laboratory of Biometrology, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, P.R. China.
authors.forename: Wen
authors.initials: W
authors.lastname: Liang
authors.affiliation: Laboratory of Biometrology, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Laboratory of Biometrology, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, P.R. China.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Ren
authors.affiliation: Laboratory of Biometrology, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, P.R. China.
authors.forename: Ziying
authors.initials: Z
authors.lastname: Zou
authors.affiliation: Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P.R. China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zuo
authors.affiliation: Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P.R. China.
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
authors.affiliation: Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Laboratory of Biometrology, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, P.R. China. Electronic address: liug@simt.com.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Chemistry, College of Life and Environmental Sciences, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, P.R. China.
authors.forename: Nengqin
authors.initials: N
authors.lastname: Jia
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers; D004261:DNA Replication / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D059629:Signal-To-Noise Ratio; D019098:Telomerase / Q000235:genetics / Q000302:isolation & purification*; D016615:Telomere / Q000737:chemistry / Q000235:genetics
chemical_list: D017931:DNA Primers; D004247:DNA; D019098:Telomerase
keywords: Cancer; DNA nano-structure; Electrochemical biosensor; Spired DNA tetrahedron TS primer; Telomerase activity