title: A new ELISA for quantification of brassinosteroids in plants.
abstract: Starting from (22R,23R)-2α,3α,22,23,26-pentahydroxy-5α-cholestan-6-one 26-hemisuccinate, conjugates of 28-norcastasterone with horse radish peroxidase and bovine serum albumin were prepared. The latter conjugate was injected into rabbits; produced polyclonal antibodies were used to quantitate 6-keto-brassinosteroids. The newly developed analytical system was used in combination with two other immunoenzymatic assays for brassinosteroids to determine individual compounds of this series. In addition, a direct method of brassinosteroid analysis was proposed. It has the advantage of requiring no sample pretreatment steps such as extraction with organic solvents and chromatography.
authors.affiliation: Hemma-Test Ltd., Masherova St., 11, 220029 Minsk, Belarus.
authors.forename: Andrey G
authors.initials: AG
authors.lastname: Pradko
authors.affiliation: Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich St., 5/2, 220141 Minsk, Belarus.
authors.forename: Raisa P
authors.initials: RP
authors.lastname: Litvinovskaya
authors.affiliation: Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich St., 5/2, 220141 Minsk, Belarus.
authors.forename: Alina L
authors.initials: AL
authors.lastname: Sauchuk
authors.affiliation: Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich St., 5/2, 220141 Minsk, Belarus.
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Drach
authors.affiliation: Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich St., 5/2, 220141 Minsk, Belarus.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Baranovsky
authors.affiliation: Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich St., 5/2, 220141 Minsk, Belarus.
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Zhabinskii
authors.affiliation: Hemma-Test Ltd., Masherova St., 11, 220029 Minsk, Belarus.
authors.forename: Tatyana V
authors.initials: TV
authors.lastname: Mirantsova
authors.affiliation: Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich St., 5/2, 220141 Minsk, Belarus. Electronic address: khripach@iboch.bas-net.by.
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Khripach
mesh_terms: D000818:Animals; D060406:Brassinosteroids / Q000032:analysis*; D002417:Cattle; D020843:Chamomile / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D006736:Horses; D008968:Molecular Conformation; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism
chemical_list: D060406:Brassinosteroids; D012710:Serum Albumin, Bovine; D006735:Horseradish Peroxidase
keywords: Brassinosteroids; Flores Chamomillae; HPLC; Phytohormones; Synthesis; ЕLISA