title: Noggin versus basic fibroblast growth factor on the differentiation of human embryonic stem cells.
abstract: The difference between Noggin and basic fibroblast growth factor for the neural precursor differentiation from human embryonic stem cells has not been studied. In this study, 100 μg/L Noggin or 20 μg/L basic fibroblast growth factor in serum-free neural induction medium was used to differentiate human embryonic stem cells H14 into neural precursors using monolayer differentiation. Two weeks after induction, significantly higher numbers of neural rosettes formed in the Noggin-induced group than the basic fibroblast growth factor-induced group, as detected by phase contrast microscope. Immunofluorescence staining revealed expression levels of Nestin, β-III Tubulin and Sox-1 were higher in the induced cells and reverse-transcription PCR showed induced cells expressed Nestin, Sox-1 and Neurofilament mRNA. Protein and mRNA expression in the Noggin-induced group was increased compared with the basic fibroblast growth factor-induced group. Noggin has a greater effect than basic fibroblast growth factor on the induction of human embryonic stem cell differentiation into neural precursors by monolayer differentiation, as Noggin accelerates and increases the differentiation of neural precursors.
authors.affiliation: Department of Laboratory Animal Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Tissue Engineering Laboratory, Shanghai Institute for Pediatric Research, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China.
authors.forename: Junmei
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Laboratory Animal Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Zhenfu
authors.initials: Z
authors.lastname: Fang
authors.affiliation: Department of Laboratory Animal Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Manxi
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Department of Laboratory Animal Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Xuejin
authors.initials: X
authors.lastname: Chen
keywords: Noggin; basic fibroblast growth factor; grants-supported paper; human embryonic stem cells; neural differentiation; neural precursors; neural regeneration; neuroregeneration; stem cells