title: Transient expression of RAD51 in the late G2-phase is required for cell cycle progression in synchronous Physarum cells.
abstract: The homologous recombination factor RAD51 is highly conserved. This criterion enabled us to identify a RAD51 ortholog in Physarum polycephalum. We found that the Physarum protein presents a high homology to the human protein and cross-reacted with antibodies directed against the human RAD51. Taking advantage of the natural synchrony of millions of nuclei within a single cell of Physarum, we investigated the fluctuation of the amount of the PpRAD51 throughout the cell cycle. Our results showed that in the late G2-phase, RAD51 was transiently expressed in a large quantity. Furthermore, knocking-down RAD51 in the G2-phase abolished this transient expression before mitosis and affected cell cycle progression. These results support the idea that RAD51 plays a role in the progression of the cell cycle in the late G2-phase.
authors.affiliation: Faculté des Sciences et des Techniques, UFIP UMR CNRS 6286 & Université de Nantes, 44322, Nantes Cedex 3, France; Division of Mechanism and Regulation of DNA Repair, Faculté des Sciences et des Techniques, UFIP UMR CNRS 6286 & Université de Nantes, 44322, Nantes Cedex 3, France; Division of Epigenetics: Proliferation and Differentiation, Faculté des Sciences et des Techniques, UFIP UMR CNRS 6286 & Université de Nantes, 44322, Nantes Cedex 3, France.
authors.forename: Anthony
authors.initials: A
authors.lastname: Le Cigne
authors.forename: Vanessa
authors.initials: V
authors.lastname: Menil-Philippot
authors.forename: Fabrice
authors.initials: F
authors.lastname: Fleury
authors.forename: Masayuki
authors.initials: M
authors.lastname: Takahashi
authors.forename: Christophe
authors.initials: C
authors.lastname: Thiriet
mesh_terms: D016195:G2 Phase; D006801:Humans; D010804:Physarum / Q000166:cytology / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism; D051135:Rad51 Recombinase / Q000235:genetics / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; D051135:Rad51 Recombinase