title: Which form of collagen is suitable for nerve cell culture?
abstract: In this study, we investigated the effects of hydrolyzed and non-hydrolyzed collagen and two-dimensional and three-dimensional collagen matrices on cell survival, attachment and neurite outgrowth of primary cultured nerve cells using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay and inverted microscopy. Hydrolyzed collagen facilitated nerve cell survival and neurite outgrowth, but it had no obvious influences on cell attachment. In contrast, non-hydrolyzed two-dimensional collagen matrix had no obvious effects on neurite outgrowth. These findings suggest that hydrolyzed collagen is an ideal nerve cell culture media.
authors.affiliation: Razi Vaccine and Serum Research Institute, Mashhad, Iran.
authors.forename: Mohsen Fathi
authors.initials: MF
authors.lastname: Najafi
authors.affiliation: Departamnt of Biology, University of Mohaghegh Ardebili, Ardebil, Iran.
authors.forename: Saber
authors.initials: S
authors.lastname: Zahri
authors.affiliation: Razi Vaccine and Serum Research Institute, Mashhad, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Vahedi
authors.affiliation: Inflammation and Inflammatory Diseases Research Center, Mashhad University of Medical Science, Mashhad, Iran.
authors.forename: Leila Esmaililian
authors.initials: LE
authors.lastname: Toosi
authors.affiliation: Departamnt of Biology, University of Mohaghegh Ardebili, Ardebil, Iran ; Inflammation and Inflammatory Diseases Research Center, Mashhad University of Medical Science, Mashhad, Iran.
authors.forename: Nazila
authors.initials: N
authors.lastname: Ariaee
keywords: cell attachment; collagen; grants-supported paper; growth; matrix; nerve cells; neural regeneration; neuroregeneration; primary culture; survival