title: Molecular characterization of yam virus X, a new potexvirus infecting yams (Dioscorea spp) and evidence for the existence of at least three distinct potexviruses infecting yams.
abstract: The genome of yam virus X (YVX), a new member of the genus Potexvirus from yam (Dioscorea trifida), was completely sequenced. Structural and phylogenetic analysis showed that the closest relative of YVX is nerine virus X. A prevalence study found YVX only in plants maintained in Guadeloupe and showed that it also infects members of the complex D. cayenensis rotundata. This study provides evidence for the existence of two additional potexviruses, one of which infects D. nummularia in Vanuatu and the other, D. bulbifera and D. rotundata in Haiti and D. trifida and D. rotundata in Guadeloupe. This work also shows that existing potexvirus-specific degenerate primers targeting the ORF1-encoded polymerase domain are well suited for the identification of the three potexviruses reported here.
authors.affiliation: CIRAD, UMR AGAP, Station de Neufchâteau, Sainte-Marie, 97130, Capesterre-Belle Eau (Guadeloupe), France.
authors.forename: Isabelle Acina
authors.initials: IA
authors.lastname: Mambole
authors.forename: Lydiane
authors.initials: L
authors.lastname: Bonheur
authors.forename: Laurence Svanella
authors.initials: LS
authors.lastname: Dumas
authors.forename: Denis
authors.initials: D
authors.lastname: Filloux
authors.forename: Rose-Marie
authors.initials: RM
authors.lastname: Gomez
authors.forename: Chantal
authors.initials: C
authors.lastname: Faure
authors.forename: David
authors.initials: D
authors.lastname: Lange
authors.forename: Fabiola
authors.initials: F
authors.lastname: Anzala
authors.forename: Claudie
authors.initials: C
authors.lastname: Pavis
authors.forename: Armelle
authors.initials: A
authors.lastname: Marais
authors.forename: Philippe
authors.initials: P
authors.lastname: Roumagnac
authors.forename: Thierry
authors.initials: T
authors.lastname: Candresse
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Teycheney
mesh_terms: D016000:Cluster Analysis; D027742:Dioscorea / Q000821:virology*; D023061:Gene Order; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017863:Potexvirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D012367:RNA, Viral