title: Medium-intensity acute exhaustive exercise induces neural cell apoptosis in the rat hippocampus.
abstract: The present study assessed the influence of medium-intensity (treadmill at a speed of 19.3 m/min until exhaustion) and high-intensity (treadmill at a speed of 26.8 m/min until exhaustion) acute exhaustive exercise on rat hippocampal neural cell apoptosis. TUNEL staining showed significantly increased neural cell apoptosis in the hippocampal CA1 region of rats after medium- and high-intensity acute exhaustive exercise, particularly the medium-intensity acute exhaustive exercise, when compared with the control. Immunohistochemistry showed significantly increased expression of the antiapoptotic protein Bcl-2 and the proapoptotic protein Bax in the hippocampal CA1 region of rats after medium- and high-intensity acute exhaustive exercise. Additionally, the ratio of Bax to Bcl-2 increased in both exercise groups. In particular, the medium-intensity acute exhaustive exercise group had significantly higher Bax and Bcl-2 protein expression and a higher Bax/Bcl-2 ratio. These findings indicate that acute exhaustive exercise of different intensities can induce neural cell apoptosis in the hippocampus, and that medium-intensity acute exhaustive exercise results in greater damage when compared with high-intensity exercise.
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha 410081, Hunan Province, China ; School of Biological Science and Technology, Central South University, Changsha 410013, Hunan Province, China.
authors.forename: Shanni
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha 410081, Hunan Province, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha 410081, Hunan Province, China.
authors.forename: Hengmei
authors.initials: H
authors.lastname: Yan
keywords: Bax; Bcl-2; acute exhaustive exercise; apoptosis; brain injury; different intensities; grant-supported paper; hippocampus; neural regeneration; neuron; neuroregeneration; photographs-containing paper