title: Single-copy nuclear gene primers for Streptanthus and other Brassicaceae from genomic scans, published data, and ESTs.
abstract: OBJECTIVE
  We report 11 primer sets for nine single-copy nuclear genes in Streptanthus and other Thelypodieae (Brassicaceae) and their utility at tribal-level and species-level phylogenetics in this poorly resolved group. •
  
  
  RESULTS
  We selected regions based on a cross-referenced matrix of previous studies and public Brassica expressed sequence tags. To design primers, we used alignments of low-depth-coverage Illumina sequencing of genomic DNA for two species of Brassica mapped onto Arabidopsis thaliana. We report several primer combinations for five regions that consistently amplified a single band and yielded high-quality sequences for at least 70% of the species assayed, and for four additional regions whose utility might be clade specific. •
  
  
  CONCLUSIONS
  Our primers will be useful in improving resolution at shallow depths across the Thelypodieae, and likely in other Brassicaceae.
authors.affiliation: Department of Evolution and Ecology, University of California, Davis, One Shields Avenue, Davis, California 95616-5294 USA.
authors.forename: N Ivalú
authors.initials: NI
authors.lastname: Cacho
authors.affiliation: Department of Evolution and Ecology, University of California, Davis, One Shields Avenue, Davis, California 95616-5294 USA.
authors.forename: Sharon Y
authors.initials: SY
authors.lastname: Strauss
keywords: Brassicaceae; Illumina; Thelypodieae; rapid diversification; single-copy nuclear gene; species-level phylogeny