title: The evaluation of angiotensin-converting enzyme (ACE) gene I/D and IL-4 gene intron 3 VNTR polymorphisms in coronary artery disease.
abstract: OBJECTIVE
  Genetic polymorphism is a strong risk factor for coronary artery disease (CAD). In the present study, our aim was to evaluate angiotensin-converting enzyme (ACE) gene I/D polymorphism and interleukin-4 (IL-4) gene Intron 3 variable number of tandem repeat (VNTR) polymorphism in CAD.
  
  
  METHODS
  One hundred and twenty-four CAD patients and one hundred and twenty-three controls were enrolled. Genomic DNA was isolated and genotyped using polymerase chain reaction (PCR) analyses.
  
  
  RESULTS
  The risk associated with inheriting the combined genotypes for the two polymorphisms were evaluated and it was found that the individuals who were P2P2-homozygous at IL-4 gene intron 3 VNTR and DD-homozygous at ACE gene I/D have a higher risk of developing CAD.
  
  
  CONCLUSIONS
  Although, there is no correlation between IL4 VNTR polymorphism and ACE gene polymorphism and CAD, there is a strong association between CAD and co-existence of IL-4 VNTR and ACE gene polymorphisms in the Turkish population.
authors.affiliation: Gaziosmanpasa University, Faculty of Medicine, Department of Emergency Medicine, Tokat, Turkey drnursahbs@hotmail.com nursahbsl@gmail.com.
authors.forename: Nursah
authors.initials: N
authors.lastname: Basol
authors.affiliation: Gaziosmanpasa University, Faculty of Medicine, Department of Cardiology, Tokat, Turkey.
authors.forename: Atac
authors.initials: A
authors.lastname: Celik
authors.affiliation: Gaziosmanpasa University, Faculty of Medicine, Department of Medical Biology, Tokat, Turkey.
authors.forename: Nevin
authors.initials: N
authors.lastname: Karakus
authors.forename: Sibel Demir
authors.initials: SD
authors.lastname: Ozturk
authors.affiliation: Gaziosmanpasa University, Faculty of Medicine, Department of Medical Biology, Tokat, Turkey.
authors.forename: Sibel Demir
authors.initials: SD
authors.lastname: Ozsoy
authors.affiliation: Gaziosmanpasa University, Faculty of Medicine, Department of Medical Biology, Tokat, Turkey.
authors.forename: Serbulent
authors.initials: S
authors.lastname: Yigit
mesh_terms: D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D054643:INDEL Mutation; D015847:Interleukin-4 / Q000235:genetics*; D007438:Introns; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats; D016017:Odds Ratio; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D011110:Polymorphism, Genetic; D012307:Risk Factors
chemical_list: D015847:Interleukin-4; D007703:Peptidyl-Dipeptidase A
keywords: ACE gene I/D polymorphism; Coronary artery disease; IL4 VNTR polymorphism