title: Sub-genomic RNA of defective interfering (D.I.) dengue viral particles is replicated in the same manner as full length genomes.
abstract: The predicted secondary structure of sub-genomic RNA in dengue virus defective interfering (D.I.) particles from patients, or generated in vitro, resembled that of the 3' and 5' regions of wild type dengue virus (DENV) genomes. While these structures in the sub-genomic RNA were found to be essential for its replication, their nucleotide sequences were not, so long as any new sequences maintained wild type RNA secondary structure. These observations suggested that these sub-genomic fragments of RNA from dengue viruses were replicated in the same manner as the full length genomes of their wild type, "helper", viruses and that they probably represent the smallest fragments of DENV RNA that can be replicated during a natural infection. While D.I. particles containing sub-genomic RNA are completely parasitic, the relationship between wild type and D.I. DENV may be symbiotic, with the D.I. particles enhancing the transmission of infectious DENV.
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, Brisbane, 4059, Australia.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Li
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, Brisbane, 4059, Australia. Electronic address: j.aaskov@qut.edu.au.
authors.forename: John
authors.initials: J
authors.lastname: Aaskov
mesh_terms: D000818:Animals; D002460:Cell Line; D009033:Culicidae / Q000166:cytology; D003673:Defective Viruses; D003716:Dengue Virus / Q000235:genetics / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D016679:Genome, Viral / Q000502:physiology*; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D012367:RNA, Viral
keywords: Defective interfering particles; Dengue virus; Disease attenuation; RNA secondary structure; Replication