title: Chimpanzee adenovirus vaccine generates acute and durable protective immunity against ebolavirus challenge.
abstract: Ebolavirus disease causes high mortality, and the current outbreak has spread unabated through West Africa. Human adenovirus type 5 vectors (rAd5) encoding ebolavirus glycoprotein (GP) generate protective immunity against acute lethal Zaire ebolavirus (EBOV) challenge in macaques, but fail to protect animals immune to Ad5, suggesting natural Ad5 exposure may limit vaccine efficacy in humans. Here we show that a chimpanzee-derived replication-defective adenovirus (ChAd) vaccine also rapidly induced uniform protection against acute lethal EBOV challenge in macaques. Because protection waned over several months, we boosted ChAd3 with modified vaccinia Ankara (MVA) and generated, for the first time, durable protection against lethal EBOV challenge.
authors.affiliation: Vaccine Research Center, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, Maryland, USA.
authors.forename: Daphne A
authors.initials: DA
authors.lastname: Stanley
authors.affiliation: 1] United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA. [2] Integrated Research Facility, NIAID, NIH, Fort Detrick, Maryland, USA (A.N.H., J.C.J. and L.H.), and Sanofi, Cambridge, Massachusetts, USA (G.J.N.).
authors.forename: Anna N
authors.initials: AN
authors.lastname: Honko
authors.affiliation: 1] Vaccine Research Center, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, Maryland, USA. [2].
authors.forename: Clement
authors.initials: C
authors.lastname: Asiedu
authors.affiliation: United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA.
authors.forename: John C
authors.initials: JC
authors.lastname: Trefry
authors.affiliation: Vaccine Research Center, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, Maryland, USA.
authors.forename: Annie W
authors.initials: AW
authors.lastname: Lau-Kilby
authors.affiliation: 1] United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA. [2] Integrated Research Facility, NIAID, NIH, Fort Detrick, Maryland, USA (A.N.H., J.C.J. and L.H.), and Sanofi, Cambridge, Massachusetts, USA (G.J.N.).
authors.forename: Joshua C
authors.initials: JC
authors.lastname: Johnson
authors.affiliation: 1] United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA. [2] Integrated Research Facility, NIAID, NIH, Fort Detrick, Maryland, USA (A.N.H., J.C.J. and L.H.), and Sanofi, Cambridge, Massachusetts, USA (G.J.N.).
authors.forename: Lisa
authors.initials: L
authors.lastname: Hensley
authors.affiliation: Okairos, Rome, Italy.
authors.forename: Virginia
authors.initials: V
authors.lastname: Ammendola
authors.affiliation: Okairos, Rome, Italy.
authors.forename: Adele
authors.initials: A
authors.lastname: Abbate
authors.affiliation: Okairos, Rome, Italy.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Grazioli
authors.affiliation: Vaccine Research Center, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, Maryland, USA.
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Foulds
authors.affiliation: Vaccine Research Center, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, Maryland, USA.
authors.forename: Cheng
authors.initials: C
authors.lastname: Cheng
authors.affiliation: Vaccine Research Center, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, Maryland, USA.
authors.forename: Lingshu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Vaccine Research Center, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, Maryland, USA.
authors.forename: Mitzi M
authors.initials: MM
authors.lastname: Donaldson
authors.affiliation: Okairos, Rome, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Colloca
authors.affiliation: Okairos, Rome, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Folgori
authors.affiliation: Vaccine Research Center, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, Maryland, USA.
authors.forename: Mario
authors.identifier: 0000000174487040
authors.initials: M
authors.lastname: Roederer
authors.affiliation: 1] Vaccine Research Center, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, Maryland, USA. [2] Integrated Research Facility, NIAID, NIH, Fort Detrick, Maryland, USA (A.N.H., J.C.J. and L.H.), and Sanofi, Cambridge, Massachusetts, USA (G.J.N.).
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Nabel
authors.affiliation: Vaccine Research Center, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, Maryland, USA.
authors.forename: John
authors.initials: J
authors.lastname: Mascola
authors.affiliation: 1] Okairos, Rome, Italy. [2] Centro Ingegneria Genetica (CEINGE), Naples, Italy. [3] Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Naples, Italy.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Nicosia
authors.affiliation: Keires AG, Basel, Switzerland.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Cortese
authors.affiliation: Vaccine Research Center, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, Maryland, USA.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Koup
authors.affiliation: Vaccine Research Center, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, Maryland, USA.
authors.forename: Nancy J
authors.initials: NJ
authors.lastname: Sullivan
mesh_terms: D062705:Adenovirus Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D000260:Adenoviruses, Human / Q000235:genetics / Q000276:immunology; D000261:Adenoviruses, Simian / Q000235:genetics / Q000276:immunology; D000818:Animals; D003673:Defective Viruses / Q000235:genetics / Q000276:immunology; D046129:Ebola Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D029043:Ebolavirus / Q000235:genetics / Q000276:immunology*; D005260:Female; D005822:Genetic Vectors; D019142:Hemorrhagic Fever, Ebola / Q000276:immunology* / Q000517:prevention & control* / Q000821:virology; D006801:Humans; D007117:Immunization, Secondary; D008252:Macaca fascicularis; D002679:Pan troglodytes; D012367:RNA, Viral / Q000097:blood / Q000235:genetics; D013997:Time Factors; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology
chemical_list: D062705:Adenovirus Vaccines; D046129:Ebola Vaccines; D012367:RNA, Viral