title: Single-molecule correlated chemical probing of RNA.
abstract: Complex higher-order RNA structures play critical roles in all facets of gene expression; however, the through-space interaction networks that define tertiary structures and govern sampling of multiple conformations are poorly understood. Here we describe single-molecule RNA structure analysis in which multiple sites of chemical modification are identified in single RNA strands by massively parallel sequencing and then analyzed for correlated and clustered interactions. The strategy thus identifies RNA interaction groups by mutational profiling (RING-MaP) and makes possible two expansive applications. First, we identify through-space interactions, create 3D models for RNAs spanning 80-265 nucleotides, and characterize broad classes of intramolecular interactions that stabilize RNA. Second, we distinguish distinct conformations in solution ensembles and reveal previously undetected hidden states and large-scale structural reconfigurations that occur in unfolded RNAs relative to native states. RING-MaP single-molecule nucleic acid structure interrogation enables concise and facile analysis of the global architectures and multiple conformations that govern function in RNA.
authors.affiliation: Departments of Chemistry.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Homan
authors.affiliation: Biomedical Engineering, and.
authors.forename: Oleg V
authors.initials: OV
authors.lastname: Favorov
authors.affiliation: Departments of Chemistry.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Lavender
authors.affiliation: Department of Computer Engineering, Istanbul University, Avcilar, Istanbul 34320, Turkey.
authors.forename: Olcay
authors.initials: O
authors.lastname: Kursun
authors.affiliation: Departments of Chemistry.
authors.forename: Xiyuan
authors.initials: X
authors.lastname: Ge
authors.affiliation: Departments of Chemistry.
authors.forename: Steven
authors.initials: S
authors.lastname: Busan
authors.affiliation: Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599; and.
authors.forename: Nikolay V
authors.initials: NV
authors.lastname: Dokholyan
authors.affiliation: Departments of Chemistry, weeks@unc.edu.
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Weeks
mesh_terms: D004926:Escherichia coli / Q000737:chemistry*; D001411:Geobacillus stearothermophilus / Q000737:chemistry*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000737:chemistry*; D016053:RNA, Protozoan / Q000737:chemistry*; D013768:Tetrahymena / Q000737:chemistry*
chemical_list: D012329:RNA, Bacterial; D016053:RNA, Protozoan
keywords: dimethyl sulfate; motif discovery; spectral clustering; structure refinement