title: Change in ploidy status from hyperdiploid to near-tetraploid in multiple myeloma associated with bortezomib/lenalidomide resistance.
abstract: Ploidy is an important prognostic factor in the risk stratification of multiple myeloma (MM) patients. Patients with MM can be divided into two groups according to the modal number of chromosomes: nonhyperdiploid (NH-MM) and hyperdiploid (H-MM), which has a more favorable outcome. The two ploidy groups represent two different oncogenetic pathways determined at the premalignant stage. The ploidy subtype also persists during the course of the disease, even during progression after the therapy, with only very rare cases of ploidy conversion. The clinical significance of ploidy conversion and its relation to drug resistance have been previously discussed. Here, we describe a female MM patient with a rare change in her ploidy status from H-MM to NH-MM, detected by cytogenetic and molecular cytogenetic examinations of consecutive bone marrow aspirates. We hypothesize that ploidy conversion (from H-MM to NH-MM) is associated with disease progression and acquired resistance to bortezomib/lenalidomide therapy.
authors.affiliation: Center of Oncocytogenetics, Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital, First Faculty of Medicine of Charles University, Prague, Czech Republic. Electronic address: lenka.pavlistova@seznam.cz.
authors.forename: Lenka
authors.initials: L
authors.lastname: Pavlistova
authors.affiliation: Center of Oncocytogenetics, Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital, First Faculty of Medicine of Charles University, Prague, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Zemanova
authors.affiliation: Center of Oncocytogenetics, Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital, First Faculty of Medicine of Charles University, Prague, Czech Republic; Department of Cytogenetics, Institute of Hematology and Blood Transfusion, Prague, Czech Republic.
authors.forename: Iveta
authors.initials: I
authors.lastname: Sarova
authors.affiliation: Center of Oncocytogenetics, Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital, First Faculty of Medicine of Charles University, Prague, Czech Republic.
authors.forename: Halka
authors.initials: H
authors.lastname: Lhotska
authors.affiliation: Center of Oncocytogenetics, Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital, First Faculty of Medicine of Charles University, Prague, Czech Republic.
authors.forename: Adela
authors.initials: A
authors.lastname: Berkova
authors.affiliation: First Medical Department, General University Hospital, First Faculty of Medicine of Charles University, Prague, Czech Republic.
authors.forename: Ivan
authors.initials: I
authors.lastname: Spicka
authors.affiliation: Center of Oncocytogenetics, Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital, First Faculty of Medicine of Charles University, Prague, Czech Republic; Department of Cytogenetics, Institute of Hematology and Blood Transfusion, Prague, Czech Republic.
authors.forename: Kyra
authors.initials: K
authors.lastname: Michalova
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D001897:Boronic Acids / Q000008:administration & dosage; D000069286:Bortezomib; D002869:Chromosome Aberrations; D055028:Comparative Genomic Hybridization; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D000077269:Lenalidomide; D008875:Middle Aged; D009101:Multiple Myeloma / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D009364:Neoplasm Recurrence, Local / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D011003:Ploidies; D011379:Prognosis; D011719:Pyrazines / Q000008:administration & dosage; D013792:Thalidomide / Q000008:administration & dosage / Q000031:analogs & derivatives
chemical_list: D001897:Boronic Acids; D007136:Immunoglobulins; D011719:Pyrazines; D013792:Thalidomide; D000069286:Bortezomib; D000077269:Lenalidomide
keywords: FISH; Multiple myeloma; drug resistance; ploidy conversion