title: Role of MAPK signal pathways in differentiation process of M2 macrophages induced by high-ambient glucose and TGF-β1.
abstract: Macrophage can be alternatively activated by TGF-β1, whether high-ambient glucose can enhance the sensitivity of TGF-β1 and the intracellular mechanisms involved in this process are not fully understood. We examined whether the mitogen-activated protein kinase is involved in the activation of macrophage induced by TGF-β1 and high-ambient glucose. The expression of arginase-1, CD206 and TGF-β1 was accessed by Western blot and immunofluorescence in RAW 264.7 cells stimulated with TGF-β1 and high-ambient glucose. The activation of MAPK pathways in the process was investigated by Western blot. The role of MAPK was assessed using biochemical inhibitors. The protein of arginase-1, CD206 and TGF-β1 was significantly overexpressed in RAW264.7 cells stimulated by TGF-β1 and high-ambient glucose. ERK and JNK phosphorylation occurred in 30 min and p38MAPK phosphorylation occurred in 30 min and 24 h after the stimulation. And the expression of arginase-1 and TGF-β1 was partially blocked by the pretreated ERK biochemical inhibitor (U0126) instead of the JNK inhibitor (SP600125) and p38MAPK inhibitor (SB203580). In conclusion, high-ambient glucose can enhance the sensitivity of TGF-β1 in RAW264.7 cells, which resulted in overexpression of TGF-β1 and arginase-1 in macrophages. ERK plays a role in this process.
authors.affiliation: a Department of Nephrology , The First Affiliated Hospital, Sun Yat-sen University , Guangzhou , China .|b Key Laboratory of Nephrology , Ministry of Health, the First Affiliated Hospital, Sun Yat-sen University , Guangzhou , China , and.|c Department of Nephrology , The Sixth Affiliated Hospital, Sun Yat-sen University , Guangzhou , China.
authors.forename: Ning
authors.initials: N
authors.lastname: Su
authors.affiliation: a Department of Nephrology , The First Affiliated Hospital, Sun Yat-sen University , Guangzhou , China .|b Key Laboratory of Nephrology , Ministry of Health, the First Affiliated Hospital, Sun Yat-sen University , Guangzhou , China , and.|c Department of Nephrology , The Sixth Affiliated Hospital, Sun Yat-sen University , Guangzhou , China.
authors.forename: Yafang
authors.initials: Y
authors.lastname: Li
authors.affiliation: a Department of Nephrology , The First Affiliated Hospital, Sun Yat-sen University , Guangzhou , China .|b Key Laboratory of Nephrology , Ministry of Health, the First Affiliated Hospital, Sun Yat-sen University , Guangzhou , China , and.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: a Department of Nephrology , The First Affiliated Hospital, Sun Yat-sen University , Guangzhou , China .|b Key Laboratory of Nephrology , Ministry of Health, the First Affiliated Hospital, Sun Yat-sen University , Guangzhou , China , and.
authors.forename: Jinjin
authors.initials: J
authors.lastname: Fan
authors.affiliation: a Department of Nephrology , The First Affiliated Hospital, Sun Yat-sen University , Guangzhou , China .|b Key Laboratory of Nephrology , Ministry of Health, the First Affiliated Hospital, Sun Yat-sen University , Guangzhou , China , and.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.affiliation: a Department of Nephrology , The First Affiliated Hospital, Sun Yat-sen University , Guangzhou , China .|b Key Laboratory of Nephrology , Ministry of Health, the First Affiliated Hospital, Sun Yat-sen University , Guangzhou , China , and.
authors.forename: Wenxing
authors.initials: W
authors.lastname: Peng
authors.affiliation: a Department of Nephrology , The First Affiliated Hospital, Sun Yat-sen University , Guangzhou , China .|b Key Laboratory of Nephrology , Ministry of Health, the First Affiliated Hospital, Sun Yat-sen University , Guangzhou , China , and.
authors.forename: Xueqing
authors.initials: X
authors.lastname: Yu
authors.affiliation: c Department of Nephrology , The Sixth Affiliated Hospital, Sun Yat-sen University , Guangzhou , China.
authors.forename: Tian-Biao
authors.initials: TB
authors.lastname: Zhou
authors.affiliation: a Department of Nephrology , The First Affiliated Hospital, Sun Yat-sen University , Guangzhou , China .|b Key Laboratory of Nephrology , Ministry of Health, the First Affiliated Hospital, Sun Yat-sen University , Guangzhou , China , and.|c Department of Nephrology , The Sixth Affiliated Hospital, Sun Yat-sen University , Guangzhou , China.
authors.forename: Zongpei
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D005947:Glucose / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000502:physiology*; D008262:Macrophage Activation / Q000502:physiology; D008264:Macrophages / Q000166:cytology* / Q000502:physiology*; D051379:Mice; D000067996:RAW 264.7 Cells; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D053773:Transforming Growth Factor beta1; D005947:Glucose
keywords: ERK; RAW264.7 cells; TGF-β1; high-ambient glucose; macrophage