title: Analysis of eight glycols in serum using LC-ESI-MS-MS.
abstract: A liquid chromatography coupled with electrospray tandem mass spectrometry method was developed for the analysis of ethylene glycol, diethylene glycol, triethylene glycol, 1,4-butanediol, 1,2-butanediol, 2,3-butanediol, 1,2-propanediol and 1,3-propanediol, in serum after a Schotten-Baumann derivatization by benzoyl chloride. Usual validation parameters were tested: linearity, repeatability and intermediate precision, limits of detection and quantification, carry over and ion suppression. Limits of detection were between 0.18 and 1.1 mg/L, and limits of quantification were between 0.4 and 2.3 mg/L. Separation of isomers was possible either chromatographically or by selecting specific multiple reaction monitoring transitions. This method could be a useful tool in case of suspected intoxication with antifreeze agents, solvents, dietary supplements or some medical drug compounds.
authors.affiliation: Pharmacokinetic and Toxicology Unit, Hospital Group of Le Havre, BP 24, 76083 Le Havre cedex, France laurent.imbert@ch-havre.fr.
authors.forename: Laurent
authors.initials: L
authors.lastname: Imbert
authors.affiliation: Pharmacokinetic and Toxicology Unit, Hospital Group of Le Havre, BP 24, 76083 Le Havre cedex, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Saussereau
authors.affiliation: Pharmacokinetic and Toxicology Unit, Hospital Group of Le Havre, BP 24, 76083 Le Havre cedex, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Lacroix
mesh_terms: D002072:Butylene Glycols / Q000097:blood; D002853:Chromatography, Liquid / Q000379:methods; D019855:Ethylene Glycol / Q000097:blood; D005026:Ethylene Glycols / Q000097:blood; D006018:Glycols / Q000097:blood*; D006801:Humans; D057230:Limit of Detection; D011092:Polyethylene Glycols / Q000378:metabolism; D011409:Propylene Glycols / Q000097:blood; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002072:Butylene Glycols; D005026:Ethylene Glycols; D006018:Glycols; D011409:Propylene Glycols; C028914:triethylene glycol; D011092:Polyethylene Glycols; C026978:2,3-butylene glycol; C041787:1,3-propanediol; C013484:diethylene glycol; C039681:1,4-butanediol; D019855:Ethylene Glycol