title: In vivo effects of focused shock waves on tumor tissue visualized by fluorescence staining techniques.
abstract: Shock waves can cause significant cytotoxic effects in tumor cells and tissues both in vitro and in vivo. However, understanding the mechanisms of shock wave interaction with tissues is limited. We have studied in vivo effects of focused shock waves induced in the syngeneic sarcoma tumor model using the TUNEL assay, immunohistochemical detection of caspase-3 and hematoxylin-eosin staining. Shock waves were produced by a multichannel pulsed-electrohydraulic discharge generator with a cylindrical ceramic-coated electrode. In tumors treated with shock waves, a large area of damaged tissue was detected which was clearly differentiated from intact tissue. Localization and a cone-shaped region of tissue damage visualized by TUNEL reaction apparently correlated with the conical shape and direction of shock wave propagation determined by high-speed shadowgraphy. A strong TUNEL reaction of nuclei and nucleus fragments in tissue exposed to shock waves suggested apoptosis in this destroyed tumor area. However, specificity of the TUNEL technique to apoptotic cells is ambiguous and other apoptotic markers (caspase-3) that we used in our study did not confirmed this observation. Thus, the generated fragments of nuclei gave rise to a false TUNEL reaction not associated with apoptosis. Mechanical stress from high overpressure shock wave was likely the dominant pathway of tumor damage.
authors.affiliation: Institute of Plasma Physics AS CR, v.v.i., Za Slovankou 3, 182 00 Prague 8, Czech Republic. Electronic address: lukes@ipp.cas.cz.
authors.forename: Petr
authors.initials: P
authors.lastname: Lukes
authors.affiliation: Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University in Prague, Salmovska 1, 120 00 Prague 2, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Zeman
authors.affiliation: Institute of Animal Physiology and Genetics AS CR, v.v.i., Rumburska 89, 277 21 Libechov, Czech Republic.
authors.forename: Vratislav
authors.initials: V
authors.lastname: Horak
authors.affiliation: Institute of Plasma Physics AS CR, v.v.i., Za Slovankou 3, 182 00 Prague 8, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Hoffer
authors.affiliation: Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University in Prague, Salmovska 1, 120 00 Prague 2, Czech Republic.
authors.forename: Pavla
authors.initials: P
authors.lastname: Pouckova
authors.affiliation: Institute of Animal Physiology and Genetics AS CR, v.v.i., Rumburska 89, 277 21 Libechov, Czech Republic.
authors.forename: Monika
authors.initials: M
authors.lastname: Holubova
authors.affiliation: Institute of Pulsed Power Science, Kumamoto University, 2-39-1 Kurokami, Kumamoto, Japan.
authors.forename: S Hamid R
authors.initials: SH
authors.lastname: Hosseini
authors.affiliation: Institute of Pulsed Power Science, Kumamoto University, 2-39-1 Kurokami, Kumamoto, Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Akiyama
authors.affiliation: Institute of Plasma Physics AS CR, v.v.i., Za Slovankou 3, 182 00 Prague 8, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Sunka
authors.affiliation: Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University in Prague, Salmovska 1, 120 00 Prague 2, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Benes
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D004599:Electric Stimulation Therapy / Q000295:instrumentation / Q000379:methods*; D004801:Eosine Yellowish-(YS); D004867:Equipment Design; D005453:Fluorescence; D006416:Hematoxylin; D007150:Immunohistochemistry / Q000379:methods; D020287:In Situ Nick-End Labeling; D008297:Male; D009374:Neoplasms, Experimental / Q000473:pathology* / Q000628:therapy*; D011917:Rats, Inbred Lew
chemical_list: D053148:Caspase 3; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin
keywords: Apoptosis; Electrical discharge; Necrosis; Shock waves; Tumor damage