title: Development of microsatellite loci in Scrophularia incisa (Scrophulariaceae) and cross-amplification in congeneric species.
abstract: OBJECTIVE
  To elucidate the population genetics and phylogeography of Scrophularia incisa, microsatellite primers were developed. We also applied these microsatellite markers to its closely related species S. dentata and S. kiriloviana. •
  
  
  RESULTS
  Using the compound microsatellite marker technique, 12 microsatellite primers were identified in S. incisa. The number of alleles ranged from 14 to 26 when assessed in 78 individuals from four populations. With high cross-species transferability, these primers also amplified in S. dentata and S. kiriloviana. •
  
  
  CONCLUSIONS
  These results indicate that these microsatellite markers are adequate for detecting and characterizing population genetic structure in the Chinese species of sect. Tomiophyllum at fine and range-wide geographical scales.
authors.affiliation: Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Rui-Hong
authors.initials: RH
authors.lastname: Wang
authors.affiliation: Hangzhou Botanical Garden, Hangzhou 310013, People's Republic of China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Chen
authors.affiliation: Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Pan
authors.initials: P
authors.lastname: Li
authors.affiliation: Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China ; Key Laboratory of Biological Resources and Conservation and Application, Talimu University, Xinjiang, People's Republic of China.
authors.forename: Cheng-Xin
authors.initials: CX
authors.lastname: Fu
keywords: Qinghai–Tibet Plateau; Scrophularia dentata; Scrophularia kiriloviana; genetic diversity; medicinal herb; microsatellite