title: Brain distribution pharmacokinetics and integrated pharmacokinetics of Panax Notoginsenoside R1, Ginsenosides Rg1, Rb1, Re and Rd in rats after intranasal administration of Panax Notoginseng Saponins assessed by UPLC/MS/MS.
abstract: Panax notoginseng saponins (PNS) constitute the main active components of a traditional Chinese medicine, Panax notoginseng (Burk.) F.H. Chen (Sanqi). To investigate brain distribution of Panax Notoginsenoside R1, Ginsenosides Rg1, Rb1, Re, and Rd, and the integrated PNS in rats, their contents in cortex, striatum, hypothalamus, medulla oblongata, hippocampus and olfactory bulb were simultaneously measured by UPLC-MS/MS. Sample preparation was carried out by the protein precipitation technique with an internal Digoxin standard. The method described here was highly efficient, with short run time, excellent specificity and sensitivity, and successfully applied for pharmacokinetics studies. NGR1, GRg1, GRb1, GRe and GRd from PNS have been detected in all six brain regions studied and quantified accurately. These findings provide more insight for further understanding of the main ways from the nasal cavity to brain as well as the migration of nasally applied drugs into the CNS parenchyma.
authors.affiliation: Department of Industrial Pharmacy, School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, PR China.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Industrial Pharmacy, School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, PR China.
authors.forename: Pengyue
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Industrial Pharmacy, School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Industrial Pharmacy, School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, PR China.
authors.forename: Yanke
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Department of Industrial Pharmacy, School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, PR China.
authors.forename: Huichao
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Industrial Pharmacy, School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Industrial Pharmacy, School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, PR China. Electronic address: dushouying@263.net.
authors.forename: Shouying
authors.initials: S
authors.lastname: Du
authors.affiliation: Department of Industrial Pharmacy, School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, PR China. Electronic address: landocean28@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000493:pharmacokinetics; D036145:Ginsenosides / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics*; D016014:Linear Models; D008297:Male; D053723:Panax notoginseng / Q000737:chemistry*; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D036145:Ginsenosides
keywords: Brain distribution; Integrated pharmacokinetics; Intranasal administration; Panax Notoginseng Saponins; Pharmacokinetics