title: [Comparative analysis of isolation methods of DNA from rhizosphere soil for research of microbiocenosis].
abstract: The comparative analysis of different methods for the isolation of microbial DNA from ordinary black earth and dark-gray podzolic soil of soybean rhizosphere was carried out. All preparations of DNA which isolated from dark-gray podzolic soil after purification using highly dispersed silica (AppliChem, Germany) were suitable for 16S rRNA genes amplification with universal bacterial primers 11F i 1492R. For DNA extraction from ordinary black earth suitable for PCR DNA preparations were obtained using two of the six investigated methods. Isolation of DNA using a commercial Power Soil DNA Isolation Kit (MO BIO, USA) can get a small amount of DNA suitable for molecular studies without further purification procedures.
authors.forename: S V
authors.initials: SV
authors.lastname: Vozniuk
authors.forename: L V
authors.initials: LV
authors.lastname: Tytova
authors.forename: H O
authors.initials: HO
authors.lastname: Iutyns'ka
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D018517:Plant Roots / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D058441:Rhizosphere; D012988:Soil Microbiology; D013025:Glycine max / Q000382:microbiology; D014455:Ukraine
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S