title: Analysis of the biological activities of Saccharomyces cerevisiae expressing intracellular EGF, extracellular EGF, and tagged EGF in early-weaned rats.
abstract: A growing number of studies suggest that epidermal growth factor (EGF) plays an important role in early-weaned animals. The objective of this experiment was to compare the biological activity of intracellularly expressed EGF (IE-EGF), extracellularly expressed EGF (EE-EGF), and tagged EGF (T-EGF) from Saccharomyces cerevisiae (S. cerevisiae) both in vivo and in vitro. Strains of S. cerevisiae expressing IE-EGF, EE-EGF, and T-EGF were designated INVSc1-IE(+), INVSc1-EE(+), and INVSc1-TE(-), respectively. The production performance, intestinal development, physio-biochemical indexes, and immunological function of early-weaned rats were measured in vivo to evaluate the biological activity of IE-EGF, EE-EGF, and T-EGF. In addition, the proliferation of rat enterocyte was also measured in vitro. In the in vivo experiment, the recombinant S. cerevisiae was shown to survive throughout the intestinal tract. The production performance (e.g., body weight) and intestinal development (e.g., mean villous height, crypt depth, total protein, DNA, and RNA) of the rats were significantly enhanced in the INVSc1-IE(+) group compared with the INVSc1-EE(+) and INVSc1-TE(-) groups (P < 0.05). However, the levels of lactate dehydrogenase (LDH), immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) showed no difference in the INVSc1-IE(+) group compared to the INVSc1-EE(+) and INVSc1-TE(-) groups (P > 0.05), with the only significant difference being found for creatine kinase (CK) (P < 0.05). In the in vitro experiment, the proliferation of enterocyte was significantly stimulated by both IE-EGF and EE-EGF compared with T-EGF (P < 0.05). Herein, IE-EGF is more suitable for application to early-weaned animals compared with EE-EGF and T-EGF.
authors.affiliation: College of Life Science and Technology, Southwest University for Nationalities, Chengdu City, 610041, People's Republic of China.
authors.forename: Shujin
authors.initials: S
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.forename: Huina
authors.initials: H
authors.lastname: Chen
authors.forename: Yangchun
authors.initials: Y
authors.lastname: Cao
authors.forename: Zhengfan
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jiabao
authors.initials: J
authors.lastname: Yang
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Huang
authors.forename: Chunhua
authors.initials: C
authors.lastname: Guo
mesh_terms: D000818:Animals; D001835:Body Weight; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020895:Enterocytes / Q000187:drug effects / Q000502:physiology; D004815:Epidermal Growth Factor / Q000235:genetics / Q000378:metabolism*; D007422:Intestines / Q000382:microbiology / Q000502:physiology; D050296:Microbial Viability; D008969:Molecular Sequence Data; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA
chemical_list: D011994:Recombinant Proteins; D004815:Epidermal Growth Factor