title: Evaluation of five real-time PCR assays for detection of Mycoplasma pneumoniae.
abstract: Four commercial real-time PCR assays to detect Mycoplasma pneumoniae were tested, and the results were compared with the results for an in-house approach. Despite differences of crossing threshold values of up to 4, assays were able to detect at least 20 CFU/5 μl (52 fg DNA/5 μl) of sample with the Diagenode kit showing the best clinical sensitivity.
authors.affiliation: TU Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Dresden, Germany roger.dumke@tu-dresden.de.
authors.forename: Roger
authors.initials: R
authors.lastname: Dumke
authors.affiliation: TU Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Dresden, Germany.
authors.forename: Enno
authors.initials: E
authors.lastname: Jacobs
mesh_terms: D000328:Adult; D002648:Child; D002675:Child, Preschool; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009177:Mycoplasma pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity