title: Zebrafish xenotransplantation model for cancer stem-like cell study and high-throughput screening of inhibitors.
abstract: Xenotransplantation studies are important tools for studying cancer biology, especially for assaying tumor cell malignancy and providing cancer information in vivo. Cancer stem-like cells (CSCs) have been identified in many cancer types to drive tumor growth and recurrence, from "keeping" to "keep" resistant to chemotherapy and radiation therapy. In this study, we developed the xenotransplantation of CSCs derived from the leukemia and solid tumor cell lines using the zebrafish models. In adult zebrafish, we investigated that the xenografted leukemia stem cells (LSCs) from K562 cells could proliferate in vivo and keep the cancer property by re-transplantation. As for the solid tumor, these CSCs from DU145 cells (human prostate cancer) and HepG2 cells (human liver cancer) could form the tumor mass and even metastasis after xenotransplantation. In addition, the zebrafish embryos with CSC xenotransplantation could evaluate docetaxel in vivo efficiently and be available to screen the novel inhibitors by high-throughput manner. In summary, these zebrafish xenotransplantation models devote a good platform for the CSC mechanism investigation and anti-CSC inhibitor screening.
authors.affiliation: Department of Molecular and Cellular Pharmacology, Pharmacogenomics and Pharmacoinformatics, Mie University Graduate School of Medicine, Mie, Japan.
authors.forename: Beibei
authors.initials: B
authors.lastname: Zhang
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Shimada
authors.forename: Junya
authors.initials: J
authors.lastname: Kuroyanagi
authors.forename: Yuhei
authors.initials: Y
authors.lastname: Nishimura
authors.forename: Noriko
authors.initials: N
authors.lastname: Umemoto
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nomoto
authors.forename: Taichi
authors.initials: T
authors.lastname: Shintou
authors.forename: Takeshi
authors.initials: T
authors.lastname: Miyazaki
authors.forename: Toshio
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D004354:Drug Screening Assays, Antitumor; D005260:Female; D056945:Hep G2 Cells; D057166:High-Throughput Screening Assays; D006801:Humans; D020014:K562 Cells; D008297:Male; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000378:metabolism* / Q000637:transplantation; D014183:Transplantation, Heterologous; D015027:Zebrafish
chemical_list: D000970:Antineoplastic Agents