title: Use of polycaprolactone (PCL) as scaffolds for the regeneration of nerve tissue.
abstract: Adipose tissue is an easily accessible source of stem cells for use in tissue regenerative medicine. In the literature, different methods have been used to stimulate acquisition of neuronal characteristics by adipose-derived stem cells (ADSC). Herein we study the growth and neuronal differentiation potential of ADSC seeded onto a porous polycaprolactone (PCL) scaffold. The objective of this study is to demonstrate that PCL can be used as a scaffold to support reconstruction of new nervous tissue using adipose stem cells. We have previously shown that undifferentiated ADSC adhere and grow on PCL. Herein we show that, after culture on PCL in neuronal differentiation medium, ADSC expressed molecular markers characteristic of neuronal cells (β-tubulin-III, Neuron-Specific Enolase (NSE), Nestin) and secrete brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF). This study suggests that PCL can be used as a scaffold to generate nervous tissue in vitro. PLC has excellent mechanical properties and a slow degradation rate. Moreover, on the basis of our results, we propose that PCL could be used for to make in vitro, scaffold coated with neuronal cells derived from Adipose stem cells (ADSC). Neuronal cells-coated PCL could find several applications to replace damaged area of ​​the body; for example, a possible use could be the generation of nerves.
authors.affiliation: Laboratory of Applied Biotechnology, Department of Anaesthesiological, Surgical and Emergency Sciences, Second University of Naples, Naples, Italy.
authors.forename: Manlio
authors.initials: M
authors.lastname: Barbarisi
authors.forename: Gerardo
authors.initials: G
authors.lastname: Marino
authors.forename: Emilia
authors.initials: E
authors.lastname: Armenia
authors.forename: Quagliariello
authors.initials: Q
authors.lastname: Vincenzo
authors.forename: Francesco
authors.initials: F
authors.lastname: Rosso
authors.forename: Marina
authors.initials: M
authors.lastname: Porcelli
authors.forename: Alfonso
authors.initials: A
authors.lastname: Barbarisi
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000328:Adult; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D020932:Nerve Growth Factor / Q000378:metabolism; D009416:Nerve Regeneration / Q000187:drug effects*; D009417:Nerve Tissue / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011091:Polyesters / Q000494:pharmacology*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D011091:Polyesters; C016240:polycaprolactone; D020932:Nerve Growth Factor
keywords: adipose stem cells; nerve; regeneration; stem cells