title: Jiaweisinisan facilitates neurogenesis in the hippocampus after stress damage.
abstract: The traditional Chinese medicine Jiaweisinisan has antidepressant effects, and can inhibit hypothalamus-pituitary-adrenal gland axis hyperactivity in stress-induced depression. In this study, rat hippocampal neural precursor cells were cultured in serum-free medium in vitro and a stress damage model was established with 120 μM corticosterone. Cells were treated with 10% (v/v) Jiaweisinisan drug-containing serum and the corticosterone antagonist RU38486. Results of the 3-(4,5-dimethylthiazol-2-yl)-3,5-di-phenytetrazoliumromide assay showed that both Jiaweisinisan drug-containing serum and RU38486 promoted the proliferation of neural precursor cells after corticosterone exposure. Immunofluorescence detection showed that after Jiaweisinisan drug-containing serum and RU38486 treatment, the 5-bromo-2-deoxyuridine/terminal deoxynucleotidyl transferase dUTP nick end labeling ratio in hippocampal neural precursor cells significantly increased, and the apoptotic rates of glial cells reduced, and neuron-like cell differentiation from neural precursor cells significantly increased. Our experimental findings indicate that Jiaweisinisan promotes hippocampal neurogenesis after stress damage.
authors.affiliation: Department of Basic Theory of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510060, Guangdong Province, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Traditional Chinese Medicine, Luzhou Medicine College, Luzhou 646000, Sichuang Province, China.
authors.forename: Chuanlian
authors.initials: C
authors.lastname: Ran
authors.affiliation: Institute of Pharmaceutical Research, Yangtze River Pharmaceutical Group Company Limited, Taizhou 225321, Jiangsu Province, China.
authors.forename: Shukao
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China.
authors.forename: Lizhen
authors.initials: L
authors.lastname: Liao
authors.affiliation: Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China.
authors.forename: Hualei
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China.
authors.forename: Weikang
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Basic Theory of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510060, Guangdong Province, China.
authors.forename: Can
authors.initials: C
authors.lastname: Yan
keywords: Jiaweisinisan; corticosterone; apoptosis; differentiation; grants-supported paper; hippocampal neural precursor cells; neural regeneration; neuroregeneration; proliferation; traditional Chinese medicine