title: Development of 13 microsatellite markers in the endangered Sinai primrose (Primula boveana, Primulaceae).
abstract: OBJECTIVE
  We developed microsatellite markers for the endangered plant Primula boveana, the Sinai primrose, and assessed the cross-transferability of these markers to six related taxa. •
  
  
  RESULTS
  DNA sequences containing microsatellites were isolated from a microsatellite-enriched library. We obtained successful amplification of 13 microsatellite primer pairs, seven of which were polymorphic in P. boveana. Eleven of these primers successfully cross-amplified to related taxa. •
  
  
  CONCLUSIONS
  The markers reported herein will be useful to characterize the genetic diversity of the endangered P. boveana and to evaluate its mating system, and have the potential to be useful for similar studies in close relatives.
authors.affiliation: Department of Botany, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt; and.
authors.forename: Hassan
authors.initials: H
authors.lastname: Mansour
authors.affiliation: Institute of Systematic Botany, University of Zurich, Zollikerstrasse 107, Zurich 8008, Switzerland.
authors.forename: Ares
authors.initials: A
authors.lastname: Jiménez
authors.affiliation: Institute of Systematic Botany, University of Zurich, Zollikerstrasse 107, Zurich 8008, Switzerland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Keller
authors.affiliation: Institute of Systematic Botany, University of Zurich, Zollikerstrasse 107, Zurich 8008, Switzerland.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Nowak
authors.affiliation: Institute of Systematic Botany, University of Zurich, Zollikerstrasse 107, Zurich 8008, Switzerland.
authors.forename: Elena
authors.initials: E
authors.lastname: Conti
keywords: Dionysia; Primula boveana; Sinai; cross-amplification; microsatellites; sect. Sphondylia.