title: Does the hepatic branch of vagus mediate the secretion of glucagon-like peptide-1 during the Roux-en-Y gastric bypass surgery?
abstract: OBJECTIVE
  The purpose of this study is to investigate the impact of the hepatic branch of the vagus and Roux-en-Y gastric bypass (RYGB) on the level of fasting and postprandial serum glucagon-like peptide-1 (GLP-1) in type 2 diabetic mellitus rats.
  
  
  METHODS
  Randomized block design, factorial experiment. Forty-five type 2 diabetic rats were divided into four groups: sham operation (S, n = 10) and sham operation with the hepatic branch of the vagotomy (SV, n = 11), Roux-en-Y gastric bypass (RYGB, n = 12) and RYGB without preservation of the vagus (RYGBV, n = 12). Levels of fasting and postprandial serum GLP-1 30 min after 50 % glucose solution (2 g/kg) by gavage were determined before surgery and postoperatively at 1, 4, and 8 weeks. Interactions between RYGB and the common hepatic branch were also assessed.
  
  
  RESULTS
  Roux-en-Y gastric bypass surgery significantly increased the concentration of postprandial serum GLP-1 and maintained it at a higher level (P < 0.05). Preservation of vagus hepatic branch only increased the concentration of postprandial serum GLP-1 at the initial stage (P < 0.05), which gradually weakened over time (P > 0.05). Both RYGB and vagotomy of the hepatic branch had no influence on fasting serum GLP-1 (P > 0.05).
  
  
  CONCLUSIONS
  During RYGB surgery for the long-term treatment of T2DM, preservation of the hepatic branch of the vagus might have no impact on serum GLP-1 level.
authors.affiliation: Department of General Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China.
authors.forename: Nian-Cun
authors.initials: NC
authors.lastname: Qiu
authors.forename: Miao-E
authors.initials: ME
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Song
authors.forename: Ying
authors.initials: Y
authors.lastname: Jiang
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Zi-Qun
authors.initials: ZQ
authors.lastname: Gu
authors.forename: Cheng-Xiang
authors.initials: CX
authors.lastname: Shan
authors.forename: Ming
authors.initials: M
authors.lastname: Qiu
mesh_terms: D000704:Analysis of Variance; D000713:Anastomosis, Roux-en-Y / Q000379:methods; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000601:surgery; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism / Q000601:surgery*; D004195:Disease Models, Animal; D015390:Gastric Bypass / Q000379:methods*; D052216:Glucagon-Like Peptide 1 / Q000378:metabolism*; D007333:Insulin Resistance; D008099:Liver / Q000294:innervation; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D013311:Streptozocin; D015996:Survival Rate; D016896:Treatment Outcome; D014628:Vagotomy; D014630:Vagus Nerve / Q000601:surgery*
chemical_list: D001786:Blood Glucose; D013311:Streptozocin; D052216:Glucagon-Like Peptide 1