title: The Pael-R gene does not mediate the changes in rotenone-induced Parkinson's disease model cells.
abstract: In this study, we established cell models for Parkinson's disease using rotenone. An RNA interference vector targeting Parkin-associated endothelin receptor-like receptor (Pael-R) was transfected into the model cells. The results of reverse-transcription polymerase chain reaction and western blot analysis showed that Pael-R expression was decreased after RNA interference compared with the control group (no treatment) and the model group (rotenone treatment), while the rate of apoptosis and survival of dopaminergic cells did not differ significantly between groups, as detected by flow cytometry and an MTT assay. These experimental findings indicate that the Pael-R gene has no role in the changes in rotenone-induced Parkinson's disease model cells.
authors.affiliation: Department of Neurology, Second Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zou
authors.affiliation: Department of Neurology, Second Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
authors.forename: Xiangqi
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Neurology, Second Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Neurology, Second Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
authors.forename: Niangui
authors.initials: N
authors.lastname: Xu
authors.affiliation: Department of Neurology, Second Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Hu
keywords: Pael-R; Parkinson's disease; RNA interference; apoptosis; mitochondria; nerve regeneration; neural regeneration; neurodegeneration; rotenone