title: Phytochemical profile of a microalgae Euglena tuba and its hepatoprotective effect against iron-induced liver damage in Swiss albino mice.
abstract: OBJECTIVE
  This study was aimed to evaluate different phytochemical constituents and the ameliorating effect of 70% methanol extract of Euglena tuba (ETME) on iron overload-induced liver injury, along with its in vitro iron-chelating and DNA protection effects.
  
  
  RESULTS
  Phytochemicals of ETME were identified by GC-MS analysis. Iron chelation and protection of Fenton reaction-induced DNA damage was conducted in vitro. Post oral administration of ETME to iron-overloaded mice, the levels of serum parameters, antioxidant enzymes, liver iron, lipid peroxidation, protein carbonyl and hydroxyproline contents were measured. ETME showed inhibition of lipid peroxidation, protein oxidation and liver fibrosis. The serum markers and liver iron were lessened, whereas enhanced levels of liver antioxidant enzymes were detected in ETME-treated group. Furthermore, the histopathological observations also substantiated the protective effects of the extract.
  
  
  CONCLUSIONS
  Several bioactive compounds identified by GC-MS may be the basis of hepatoprotective as well as antioxidant and iron-chelating effect of ETME.
  
  
  CONCLUSIONS
  Currently available iron-chelating agents show several side effects and limitations which may be overcome by ETME, which suggest its benefit against pathology of iron overload-linked diseases. Hence, ETME can be used as a promising hepatoprotective agent.
authors.affiliation: Division of Molecular Medicine, Bose Institute, Kolkata, West Bengal, India.
authors.forename: S
authors.initials: S
authors.lastname: Panja
authors.forename: D
authors.initials: D
authors.lastname: Chaudhuri
authors.forename: N B
authors.initials: NB
authors.lastname: Ghate
authors.forename: N
authors.initials: N
authors.lastname: Mandal
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002457:Cell Extracts / Q000737:chemistry / Q000627:therapeutic use; D005055:Euglena / Q000737:chemistry*; D007501:Iron / Q000378:metabolism; D007502:Iron Chelating Agents / Q000627:therapeutic use*; D019190:Iron Overload / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D058086:Microalgae / Q000737:chemistry; D010084:Oxidation-Reduction; D064209:Phytochemicals / Q000737:chemistry; D020011:Protective Agents / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D002457:Cell Extracts; D007502:Iron Chelating Agents; D064209:Phytochemicals; D020011:Protective Agents; D007501:Iron
keywords: DNA protection; Euglena tuba; GC-MS analysis; ferritin; hepatoprotection; iron overload; lipid peroxidation