title: Paternal breed effects on expression of IGF-II, BAK1 and BCL2-L1 in bovine preimplantation embryos.
abstract: The effects of the paternal breed on early embryo and later pre- and postnatal development are well documented. Several recent studies have suggested that such paternal effects may be mediated by the paternally induced epigenetic modifications during early embryogenesis. The objective of this study was to investigate the effects of the paternal breed on the early embryonic development and relative expression of the maternally imprinted gene, IGF-II, and the apoptosis-related genes BAK1 and BCL2-L1 in in vitro produced (IVP) bovine embryos derived from two unrelated paternal breeds (Holstein and Brown Swiss). The degree of correlation of IGF-II expression pattern with embryo developmental competence and apoptosis-related genes was also investigated. The relative abundance of IGF-II, BCL2-L1 and BAK1 transcripts in day 8 embryos was measured by quantitative reverse-transcription polymerase chain reaction using the comparative Cp method. Our data revealed that the paternal breed did not influence cleavage rate, blastocyst rate and relative abundance of IGF-II, BAK1 and BCL2-L1 in day 8 blastocysts (P > 0.05). Nevertheless, IGF-II expression levels were highly correlated with embryonic developmental competence (r = 0.66, P < 0.1), relative expression of BCL2-L1 (r = 0.72, P < 0.05) and ratio of BCL2-L1/BAK1 (r = 0.78, P < 0.05). In conclusion, our data show that IGF-II, BCL2-L1 and BAK1 expression is not related to the chosen combination of paternal breed, but that IGF-II expression is correlated with embryonic viability and apoptosis-related gene expression.
authors.affiliation: Department of Animal Science,Faculty of Agriculture,University of Zabol,P.O. Box 98615-538,Zabol,Iran.
authors.forename: Mehdi Vafaye
authors.initials: MV
authors.lastname: Valleh
authors.affiliation: Department of Animal Science,Faculty of Agriculture,Ferdowsi University of Mashhad,Mashhad,I.R.Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Tahmoorespur
authors.affiliation: National Institutes for Genetic Engineering and Biotechnology,Karaj,Iran.
authors.forename: Morteza Daliri
authors.initials: MD
authors.lastname: Joupari
authors.affiliation: Embryonic and Stem Cell Biology and Biotechnology Research Group,Research Institute of Biotechnology,Ferdowsi University of Mashhad,Mashhad,Iran.
authors.forename: Hesam
authors.initials: H
authors.lastname: Dehghani
authors.affiliation: Department of Basic Animal and Veterinary Sciences,Faculty of Life Sciences,University of Copenhagen,Groennegardsvej,7,1870 Frederiksberg C,Denmark.
authors.forename: Mikkel Aabech
authors.initials: MA
authors.lastname: Rasmussen
authors.affiliation: Department of Basic Animal and Veterinary Sciences,Faculty of Life Sciences,University of Copenhagen,Groennegardsvej,7,1870 Frederiksberg C,Denmark.
authors.forename: Poul
authors.initials: P
authors.lastname: Hyttel
authors.affiliation: Department of Basic Animal and Veterinary Sciences,Faculty of Life Sciences,University of Copenhagen,Groennegardsvej,7,1870 Frederiksberg C,Denmark.
authors.forename: Lotte
authors.initials: L
authors.lastname: Strøbech
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism*; D001947:Breeding; D002417:Cattle; D002478:Cells, Cultured; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D007335:Insulin-Like Growth Factor II / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism; D050998:bcl-2 Homologous Antagonist-Killer Protein / Q000235:genetics / Q000378:metabolism*; D051020:bcl-X Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D050998:bcl-2 Homologous Antagonist-Killer Protein; D051020:bcl-X Protein; D007335:Insulin-Like Growth Factor II
keywords: BAK1; BCL2-L1; Bovine blastocyst; IGF-II; Paternal breed