title: DNA methylation analysis of SFRP2, GATA4/5, NDRG4 and VIM for the detection of colorectal cancer in fecal DNA.
abstract: Aberrantly methylated genes are increasingly being established as biomarkers for the detection of colorectal cancer (CRC). In the present study, the methylation levels of the secreted frizzled-related protein gene 2 (SFRP2), GATA binding protein 4/5 (GATA4/5), N-Myc downstream-regulated gene 4 (NDRG4) and vimentin (VIM) promoters were evaluated for their use as markers in the noninvasive detection of CRC. Methylation-specific polymerase chain reaction was performed to analyze promoter CpG methylation ofSFRP2,GATA4/5,NDRG4andVIMin the fecal DNA of 56 patients with CRC and 40 individuals exhibiting normal colonoscopy results. Promoter methylation levels ofSFRP2,GATA4/5,NDRG4andVIMin CRC patients were 57.1% (32/56), 42.9% (24/56), 83.9% (47/56), 28.6% (16/56) and 41.1% (23/56), respectively. Furthermore, the specificity of the genes were 90.0% (4/40), 95.0% (2/40), 82.5% (7/40), 97.5% (4/40) and 85.0% (6/40), respectively. The overall sensitivity of detection for fecal DNA with at least one methylated gene was 96.4% (54/56) in CRC patients. By contrast, only 14 of the 40 normal individuals exhibited methylated DNA in the aforementioned promoter regions. Methylation of theSFRP2,GATA4/5,NDRG4andVIMpromoters in fecal DNA is associated with the presence of colorectal tumors. Therefore, the detection of aberrantly methylated DNA in fecal samples may present a promising, noninvasive screening method for CRC.
authors.affiliation: Ningbo Medical Center, Li Huili Hospital, Ningbo, Zhejiang 315040, P.R. China ; Medical School of Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Hongna
authors.initials: H
authors.lastname: Lu
authors.affiliation: Ningbo Medical Center, Li Huili Hospital, Ningbo, Zhejiang 315040, P.R. China.
authors.forename: Shiliang
authors.initials: S
authors.lastname: Huang
authors.affiliation: Ningbo Medical Center, Li Huili Hospital, Ningbo, Zhejiang 315040, P.R. China.
authors.forename: Xie
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Medical School of Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Danping
authors.initials: D
authors.lastname: Wang
authors.affiliation: Ningbo Medical Center, Li Huili Hospital, Ningbo, Zhejiang 315040, P.R. China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Ningbo Medical Center, Li Huili Hospital, Ningbo, Zhejiang 315040, P.R. China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Ningbo Medical Center, Li Huili Hospital, Ningbo, Zhejiang 315040, P.R. China.
authors.forename: Qiubo
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Ningbo Medical Center, Li Huili Hospital, Ningbo, Zhejiang 315040, P.R. China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Huang
keywords: colorectal cancer; fecal DNA; methylated DNA