title: In vitro α-amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of Citrus macroptera Montr. fruit.
abstract: OBJECTIVE
  To investigate the therapeutic effects of methanol extract of Citrus macroptera Montr.fruit in α-amylase inhibitory activity (in vitro) and hypoglycemic activity in normal and glucose induced hyperglycemic rats (in vivo).
  
  
  METHODS
  Fruits of Citrus macroptera without rind was extracted with pure methanol following cold extraction and tested for presence of phytochemical constituents, α-amylase inhibitory activity, and hypoglycemic effect in normal rats and glucose induced hyperglycemic rats.
  
  
  RESULTS
  Presence of saponin, steroid and terpenoid were identified in the extract. The results showed that fruit extract had moderate α-amylase inhibitory activity [IC50 value=(3.638±0.190) mg/mL] as compared to acarbose. Moreover at 500 mg/kg and 1 000 mg/kg doses fruit extract significantly (P<0.05 and P<0.01 respectively) reduced fasting blood glucose level in normal rats as compared to glibenclamide (5 mg/kg). In oral glucose tolerance test, 500 mg/kg dose significantly reduced blood glucose level (P<0.05) at 2 h but 1 000 mg/kg dose significantly reduced blood glucose level at 2 h and 3 h (P<0.05 and P<0.01 respectively) whereas glibenclamide (5 mg/kg) significantly reduced glucose level at every hour after administration. Overall time effect is also considered extremely significant with F value=23.83 and P value=0.0001 in oral glucose tolerance test.
  
  
  CONCLUSIONS
  These findings suggest that the plant may be a potential source for the development of new oral hypoglycemic agent.
authors.affiliation: Laboratory of Natural Products Research, Department of Pharmacy, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
authors.forename: Nizam
authors.initials: N
authors.lastname: Uddin
authors.affiliation: Laboratory of Natural Products Research, Department of Pharmacy, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
authors.forename: Md Rakib
authors.initials: MR
authors.lastname: Hasan
authors.affiliation: Laboratory of Natural Products Research, Department of Pharmacy, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
authors.forename: Md Monir
authors.initials: MM
authors.lastname: Hossain
authors.affiliation: Laboratory of Natural Products Research, Department of Pharmacy, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
authors.forename: Arjyabrata
authors.initials: A
authors.lastname: Sarker
authors.affiliation: Laboratory of Natural Products Research, Department of Pharmacy, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
authors.forename: A H M Nazmul
authors.initials: AH
authors.lastname: Hasan
authors.affiliation: Laboratory of Natural Products Research, Department of Pharmacy, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
authors.forename: A F M Mahmudul
authors.initials: AF
authors.lastname: Islam
authors.affiliation: Laboratory of Natural Products Research, Department of Pharmacy, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
authors.forename: Mohd Motaher H
authors.initials: MM
authors.lastname: Chowdhury
authors.affiliation: Laboratory of Natural Products Research, Department of Pharmacy, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
authors.forename: Md Sohel
authors.initials: MS
authors.lastname: Rana
keywords: Citrus macroptera; Diabetes mellitus; Glibenclamide; Hypoglycemic; OGTT; α-Amylase