title: Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression.
abstract: Agrobacterium-mediated transient expression is an approach for short-time expression of heterologous genes in plant systems. During the last decade transient expression was regarded as a potent protocol for high scale production of foreign proteins in plants including pharmaceutically valuable proteins. In vitro grown plant cell cultures represent a suitable system for accumulation of heterologous proteins under controlled conditions. Since host characteristics may strongly influence transient expression efficiency, we performed screening of undifferentiated cell cultures for transient expression ability using GUS as a reporter. Analysis of 248 plant species belonging to 49 families from the National Germplasm Bank of the World Flora of the Institute of Cell Biology and Genetic Engineering (Kyiv, Ukraine) allowed for selection of about 50 plant species exhibiting detectable beta-glucuronidase activity.
authors.forename: Y R
authors.initials: YR
authors.lastname: Sindarovska
authors.forename: I S
authors.initials: IS
authors.lastname: Golovach
authors.forename: V B
authors.initials: VB
authors.lastname: Belokurova
authors.forename: I M
authors.initials: IM
authors.lastname: Gerasymenko
authors.forename: Y V
authors.initials: YV
authors.lastname: Sheludko
authors.forename: N V
authors.initials: NV
authors.lastname: Kuchuk
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D018920:Coculture Techniques; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics*; D059828:Plant Cells / Q000201:enzymology*; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics; D013045:Species Specificity; D014170:Transformation, Genetic
chemical_list: D005966:Glucuronidase