title: Methyl-thiophanate increases reactive oxygen species production and induces genotoxicity in rat peripheral blood.
abstract: Methylthiophanate is one of the widely used fungicides to control important fungal diseases of crops. The aim of this study was to elucidate the short-term hematoxicity and genotoxicity effects of methylthiophanate administered by intraperitoneal way at three doses (300, 500 and 700 mg/kg of body weight) after 24, 48 and 72 h. Our results showed, 24 h after methylthiophanate injection, a hematological perturbation such as red blood cells (p < 0.05, p < 0.05 and p < 0.01) and hemoglobin content (p < 0.05), respectively, and a noticeable genotoxic effect in WBC evidenced by a significant increase in the frequency of the micronuclei and a decrease in cell viability. An increase in erythrocyte osmotic fragility was also noted after 24 and 48 h of methylthiophanate treatment at graded doses. A significant increase in hydrogen peroxide, advanced oxidation of protein products and malondialdehyde levels, in erythrocytes of methylthiophanate-treated rats with 300, 500 and 700 mg/kg of body weight, was also observed after 24 h of treatment (p < 0.05, p < 0.01 and p < 0.001, respectively), suggesting the implication of oxidative stress in its toxicity. Antioxidants activities of superoxide dismutase and glutathione peroxidase in erythrocytes significantly increased (p < 0.001) 24 h after the highest dose injected. While all these parameters were improved after 72 h of methylthiophanate injection (300, 500 and 700 mg/kg body weight). In conclusion, these data showed that the exposure of adult rats to methylthiophanate resulted in oxidative stress leading to hematotoxicity and the impairment of defence system, confirming the pro-oxidant and genotoxic effects of this fungicide.
authors.affiliation: Animal Physiology Laboratory, Faculty of Sciences, University of Sfax , Tunisia .
authors.forename: Ibtissem
authors.initials: I
authors.lastname: Ben Amara
authors.forename: Hajer
authors.initials: H
authors.lastname: Ben Saad
authors.forename: Boutheina
authors.initials: B
authors.lastname: Cherif
authors.forename: Awatef
authors.initials: A
authors.lastname: Elwej
authors.forename: Saloua
authors.initials: S
authors.lastname: Lassoued
authors.forename: Choumous
authors.initials: C
authors.lastname: Kallel
authors.forename: Najiba
authors.initials: N
authors.lastname: Zeghal
mesh_terms: D000818:Animals; D007928:Lethal Dose 50; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013875:Thiophanate / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D017382:Reactive Oxygen Species; D013875:Thiophanate
keywords: Genotoxicity; hematological parameters; methylthiophanate; micronucleus test; oxidative stress; rat viability test