title: Inhibition profile of a series of phenolic acids on bovine lactoperoxidase enzyme.
abstract: Lactoperoxidase (LPO) catalyzes the oxidation of numerous of organic and inorganic substrates by hydrogen peroxide. It has very vital activity in the innate immune system by decreasing or stopping the activation of the bacteria in milk and mucosal secretions. This study's purpose was to investigate in vitro effect of some phenolic acids (ellagic, gallic, ferulic, caffeic, quercetin, p-coumaric, syringic, catechol and epicatechin) on the purified LPO. This enzyme was purified from milk by using different methods such as Amberlite CG-50 resin, CM-Sephadex C-50 ion-exchange and Sephadex G-100 gel filtration chromatography. LPO was purified 28.7-fold with a yield of 20.03%. We found phenolic acids have inhibition effects on bovine LPO enzyme to different concentrations. Our study showed lower concentrations of caffeic acid, ferulic acid and quercetin exhibited much higher inhibitory effect on enzyme, so these three of them were clearly a more potent inhibitor than the others were. All of compounds were non-competitive inhibitors.
authors.affiliation: Department of Food Engineering, Faculty of Engineering and Natural Sciences, Gumushane University , Gumushane , Turkey .
authors.forename: S Beyza Ozturk
authors.initials: SB
authors.lastname: Sarikaya
authors.forename: Melda
authors.initials: M
authors.lastname: Sisecioglu
authors.forename: Murat
authors.initials: M
authors.lastname: Cankaya
authors.forename: İlhami
authors.initials: İ
authors.lastname: Gulcin
authors.forename: Hasan
authors.initials: H
authors.lastname: Ozdemir
mesh_terms: D000818:Animals; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D062385:Hydroxybenzoates / Q000737:chemistry / Q000494:pharmacology*; D007784:Lactoperoxidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015394:Molecular Structure; D013329:Structure-Activity Relationship
chemical_list: D062385:Hydroxybenzoates; D007784:Lactoperoxidase; C017616:phenolic acid
keywords: Inhibition; lactoperoxidase; milk enzyme; phenolic acids