title: The association of lectin-like oxidized LDL receptor 1 (LOX-1) K167N and 3'UTR188CT polymorphisms with maternal plasma soluble LOX-1 levels and preeclampsia risk in Turkish population.
abstract: OBJECTIVE
  To investigate the main effect of polymorphisms in genes involved in endothelial pathophysiological mechanisms, LOX-1 K167N and 3'UTR188CT single nucleotide polymorphisms (SNPs) in relation to preeclampsia (PE) risk and possible interactions between the gene polymorphisms and plasma oxLDL and soluble LOX-1 (sLOX-1) levels on PE in Turkish population.
  
  
  METHODS
  LOX-1 K167N and 3'UTR188CT polymorphisms were studied in 113 pregnant women with preeclampsia and 96 healthy pregnant women by the PCR-RFLP techniques. sLOX-1 and oxLDL levels were determined by enzyme-linked immunosorbent assay (ELISA) in all study subjects.
  
  
  RESULTS
  Patients having LOX-1 3'UTR188CT (OR 3.55, 95% CI 1.89-6.67, P = 0.001) or 3'UTR188CC (OR 3.04, 95% CI 1.25-7.38, P = 0.012) genotype had a significantly higher risk of PE than those with 3'UTR188TT genotype. Also, patients having K167N KK (OR 2.73, 95% CI 1.33-5.61, P = 0.005) genotype had a significantly higher risk of PE than those with K167N NN genotype. LOX-1 3'UTR188TT and LOX-1 K167N NN genotype carriers were associated with significantly increased serum sLOX-1 level (P = 0.001). We further investigated the potential combined effect of these polymorphic variants on risk of PE development. According to the combined genotype analysis of LOX-1 3'UTR188TT and K167N NN polymorphisms, sLOX-1 and oxLDL levels also showed significant differences between PE patients and controls with or without combined TT/NN genotype carriers.
  
  
  CONCLUSIONS
  Our findings indicate that higher plasma sLOX-1 and oxLDL concentrations, and the LOX-1 3'UTR188C>T and LOX-1 K167N gene polymorphisms were significantly associated with risk of developing preeclampsia. Plasma sLOX-1 may be a potential therapeutic target in the treatment of preeclampsia.
authors.affiliation: Department of Obstetrics and Gynecology, Cerrahpasa Medical Faculty, Istanbul University, 34098, Istanbul, Turkey.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Tuten
authors.forename: Birsen
authors.initials: B
authors.lastname: Aydemir
authors.forename: Mahmut
authors.initials: M
authors.lastname: Oncul
authors.forename: Ali Riza
authors.initials: AR
authors.lastname: Kiziler
authors.forename: Abdullah Serdar
authors.initials: AS
authors.lastname: Acıkgoz
authors.forename: Gulcan Guntas
authors.initials: GG
authors.lastname: Korkmaz
authors.forename: Volkan
authors.initials: V
authors.lastname: Sozer
authors.forename: Hafize
authors.initials: H
authors.lastname: Uzun
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005838:Genotype; D006801:Humans; D037102:Lectins / Q000235:genetics; D008077:Lipoproteins, LDL / Q000097:blood / Q000235:genetics; D062145:Maternal Serum Screening Tests; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011225:Pre-Eclampsia / Q000097:blood / Q000208:ethnology / Q000235:genetics*; D011247:Pregnancy; D012306:Risk; D051127:Scavenger Receptors, Class E / Q000097:blood / Q000235:genetics*; D014421:Turkey / Q000453:epidemiology
chemical_list: D020413:3' Untranslated Regions; D037102:Lectins; D008077:Lipoproteins, LDL; D051127:Scavenger Receptors, Class E; C099252:oxidized low density lipoprotein