title: A new strain of Wolbachia in an alpine population of the viviparous Oreina cacaliae (Coleoptera: Chrysomelidae).
abstract: Microbial symbionts played a central role in insect evolution. Oreina cacaliae (Schrank, 1785) (Coleoptera: Chrysomelidae) is a rare example of a viviparous insect, able to feed on toxic plants and sequester toxic compounds. In the current study, the microbiota associated with O. cacaliae was characterized using a culture-independent approach, targeting the 16S rRNA bacterial gene. The obtained 16S rRNA gene sequences were analyzed and identified at different taxonomic levels. Wolbachia was the dominant bacterium, both in male and female (100 and 91.9%, respectively) individuals; the detected Wolbachia was described as a new sequence type based on multilocus sequence typing (Wolbachia ST375 Ocac_A_wVdO). After phylogenetic analyses, Wolbachia ST375 Ocac_A_wVdO was attributed to the supergroup A. Immunofluorescence assays and electron microscopy confirmed the presence of Wolbachia within O. cacaliae oocytes, confirming its transovarial transmission in this species. Representatives of six species of Oreina were tested for the presence of Wolbachia through specific polymerase chain reaction, and a dendrogram was generated for these species based on coxI gene sequences. The Wolbachia harbored by different species of Oreina were characterized by multilocus sequence typing. Five out of the six examined Oreina species were positive for Wolbachia, with four of these harboring the same sequence type.
authors.affiliation: Dipartimento di Scienze Agrarie e Ambientali - Produzione, Territorio, Agroenergia, Università degli Studi di Milano, Milan, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Montagna
authors.forename: Bessem
authors.initials: B
authors.lastname: Chouaia
authors.forename: Luciano
authors.initials: L
authors.lastname: Sacchi
authors.forename: Daniele
authors.initials: D
authors.lastname: Porretta
authors.forename: Elena
authors.initials: E
authors.lastname: Martin
authors.forename: Annamaria
authors.initials: A
authors.lastname: Giorgi
authors.forename: Giuseppe Carlo
authors.initials: GC
authors.lastname: Lozzia
authors.forename: Sara
authors.initials: S
authors.lastname: Epis
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000382:microbiology* / Q000502:physiology*; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D005260:Female; D008297:Male; D058885:Multilocus Sequence Typing; D009865:Oocytes / Q000382:microbiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D012098:Reproduction; D017422:Sequence Analysis, DNA; D020577:Wolbachia / Q000145:classification / Q000235:genetics* / Q000502:physiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S