title: Mapping the genomic binding sites of the activated retinoid X receptor in murine bone marrow-derived macrophages using chromatin immunoprecipitation sequencing.
abstract: Chromatin immunoprecipitation followed by massively parallel sequencing (ChIP-Seq) is a powerful technique to map the genomic location of a given chromatin bound factor (i.e., transcription factors, cofactors) or epigenetic marks, such as histone modification. The procedure is based on cross-linking of proteins to DNA followed by the capture of the protein-DNA complexes by "ChIP-grade" antibodies. In this chapter we describe in detail the experimental method developed in our laboratory to investigate in vivo the DNA-binding characteristics of a key heterodimeric nuclear receptor, the retinoid X receptor (RXR) in murine bone marrow-derived macrophages.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Bence
authors.initials: B
authors.lastname: Daniel
authors.forename: Balint L
authors.initials: BL
authors.lastname: Balint
authors.forename: Zsuzsanna S
authors.initials: ZS
authors.lastname: Nagy
authors.forename: Laszlo
authors.initials: L
authors.lastname: Nagy
mesh_terms: D000818:Animals; D001665:Binding Sites; D001854:Bone Marrow Cells / Q000378:metabolism; D002478:Cells, Cultured; D002843:Chromatin / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016678:Genome; D023281:Genomics / Q000379:methods*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D047488:Retinoid X Receptors / Q000378:metabolism*
chemical_list: D002843:Chromatin; D047488:Retinoid X Receptors; D004247:DNA