title: Electrospinning of aniline pentamer-graft-gelatin/PLLA nanofibers for bone tissue engineering.
abstract: Blends of aniline pentamer-graft-gelatin (AP-g-GA) and poly(l-lactide) (PLLA) were electrospun to prepare uniform nanofibers as biomimetic scaffolds. The nanofibers exhibited good electroactivity, thermal stability and biodegradability. The biocompatibility of the nanofibers in vitro was evaluated by the adhesion and proliferation of mouse preosteoblastic MC3T3-E1 cells. The cellular elongation was significantly greater on electroactive AP-g-GA/PLLA nanofibers than on PLLA nanofibers. Moreover, the AP-g-GA/PLLA nanofibers stimulated by an electrical pulsed signal could promote the differentiation of MC3T3-E1 cells compared with pure PLLA nanofibers. Our results demonstrated that the biodegradable and electroactive AP-g-GA/PLLA nanofibers had potential application in vivo as bone repair scaffold materials in tissue engineering.
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Cui
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Zhuang
authors.affiliation: Department of Chemistry, Tsinghua University, Beijing 100084, China. Electronic address: weiyen@tsinghua.edu.cn.
authors.forename: Yen
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. Electronic address: xschen@ciac.jl.cn.
authors.forename: Xuesi
authors.initials: X
authors.lastname: Chen
mesh_terms: D016475:3T3 Cells; D000814:Aniline Compounds / Q000737:chemistry*; D000818:Animals; D018786:Bone Substitutes / Q000008:administration & dosage / Q000138:chemical synthesis*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D004595:Electroplating / Q000379:methods; D019544:Equipment Failure Analysis; D005780:Gelatin / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D057139:Nanofibers / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D010316:Particle Size; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011474:Prosthesis Design; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D000814:Aniline Compounds; D018786:Bone Substitutes; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D005780:Gelatin; C023650:aniline
keywords: Biocompatibility; Biodegradable; Cell differentiation; Electroactivity; Electrospinning