title: Emerging salivary biomarkers by mass spectrometry.
abstract: Human saliva, a multi-constituent oral fluid, has a high potential for early diagnosis of disease. Proteomic analysis of saliva holds promise as a non-invasive method that is advantageous over serum. This non-invasive diagnostic method represents developing trends in analytical and clinical chemistry. Significant technological advances in the field of proteomics during the last two decades have greatly facilitated the research toward this direction. However, these technologies still require integration and standardization of validation against accepted clinical and pathologic parameters. In this review, a summary of mass spectrometry-based technologies of saliva biomarker discovery, potential clinical applications, and challenges of saliva proteomics have been discussed, as well as latest technologies of validation and quantification of saliva biomarkers. It is likely that the use of saliva for early diagnostics of diseases will continue to expand thus providing a new approach of instrumental investigation for physiologic and physiological states. These novel biomarkers have obvious clinical utility that will help to diagnose many diseases at early stage.
authors.affiliation: Research Center of Analytical Instrumentation, College of Life Science, Sichuan University, Chengdu 610064, PR China; College of Chemistry and Life Sciences, Chengdu Normal University, Chengdu 610041, PR China.
authors.forename: Qihui
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Research Center of Analytical Instrumentation, College of Life Science, Sichuan University, Chengdu 610064, PR China; Department of Environmental and Food Engineering, Liuzhou Vocational and Technical College, Liuzhou 545006, PR China.
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Research Center of Analytical Instrumentation, College of Life Science, Sichuan University, Chengdu 610064, PR China.
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Research Center of Analytical Instrumentation, College of Life Science, Sichuan University, Chengdu 610064, PR China. Electronic address: yduan@scu.edu.cn.
authors.forename: Yixiang
authors.initials: Y
authors.lastname: Duan
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D004194:Disease / Q000145:classification*; D042241:Early Diagnosis; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D012463:Saliva / Q000737:chemistry*
chemical_list: D015415:Biomarkers
keywords: Biomarker; Disease diagnosis; Mass spectrometry; Non-invasive; Saliva