title: A view of bivalent epigenetic marks in two human embryonic stem cell lines reveals a different cardiogenic potential.
abstract: Human embryonic stem (HUES) cells are derived from early individual embryos with unique genetic printing. However, how their epigenetic status might affect their potential to differentiate toward specific lineages remains a puzzling question. Using chromatin immunoprecipitation (ChIP)-polymerase chain reaction and ChIP-on-chip, the status of bivalent domains on gene promoters (ie, histone 3 on lysine 4 and histone 3 on lysine 27 trimethylation) was monitored for both undifferentiated and bone morphogenetic protein 2 (BMP2)-induced cardiac-committed cells. A marked difference in the epigenetic profile of HUES cell lines was observed and this was correlated to the pattern of gene expression induced by BMP2 as well as to their potential to generate cardiac progenitors and differentiated myocytes. Thus, the epigenetic H3trimeK4 and H3trimeK27 prints generating bivalent domains on promoters, could be used to predict a preference in their differentiation toward a specific lineage.
authors.affiliation: 1 INSERM UMR 633, Genopole Evry, University Paris V Descartes , Evry, France .
authors.forename: Julia
authors.initials: J
authors.lastname: Leschik
authors.forename: Leslie
authors.initials: L
authors.lastname: Caron
authors.forename: Henry
authors.initials: H
authors.lastname: Yang
authors.forename: Chad
authors.initials: C
authors.lastname: Cowan
authors.forename: Michel
authors.initials: M
authors.lastname: Pucéat
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology / Q000502:physiology; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D002478:Cells, Cultured; D047369:Chromatin Immunoprecipitation; D018920:Coculture Techniques; D019175:DNA Methylation; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D005347:Fibroblasts; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000235:genetics; D005819:Genetic Markers; D006321:Heart / Q000196:embryology; D011495:Histone-Lysine N-Methyltransferase / Q000378:metabolism; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051379:Mice; D009206:Myocardium / Q000166:cytology; D032383:Myocytes, Cardiac / Q000166:cytology*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D005819:Genetic Markers; D006657:Histones; D014157:Transcription Factors; D011495:Histone-Lysine N-Methyltransferase