title: Stochastic particle barcoding for single-cell tracking and multiparametric analysis.
abstract: This study presents stochastic particle barcoding (SPB), a method for tracking cell identity across bioanalytical platforms. In this approach, single cells or small collections of cells are co-encapsulated within an enzymatically-degradable hydrogel block along with a random collection of fluorescent beads, whose number, color, and position encode the identity of the cell, enabling samples to be transferred in bulk between single-cell assay platforms without losing the identity of individual cells. The application of SPB is demonstrated for transferring cells from a subnanoliter protein secretion/phenotyping array platform into a microtiter plate, with re-identification accuracies in the plate assay of 96±2%. Encapsulated cells are recovered by digesting the hydrogel, allowing subsequent genotyping and phenotyping of cell lysates. Finally, a model scaling is developed to illustrate how different parameters affect the accuracy of SPB and to motivate scaling of the method to thousands of unique blocks.
authors.affiliation: Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA.
authors.forename: M
authors.initials: M
authors.lastname: Castellarnau
authors.forename: G L
authors.initials: GL
authors.lastname: Szeto
authors.forename: H-W
authors.initials: HW
authors.lastname: Su
authors.forename: T
authors.initials: T
authors.lastname: Tokatlian
authors.forename: J C
authors.initials: JC
authors.lastname: Love
authors.forename: D J
authors.initials: DJ
authors.lastname: Irvine
authors.forename: J
authors.initials: J
authors.lastname: Voldman
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D058948:Cell Tracking / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D046911:Macromolecular Substances / Q000378:metabolism; D051379:Mice; D008962:Models, Theoretical; D009696:Nucleic Acids / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry; D013269:Stochastic Processes
chemical_list: D046911:Macromolecular Substances; D009696:Nucleic Acids; C437167:poly(ethylene glycol)diacrylate; D011092:Polyethylene Glycols
keywords: barcodes; cells; hydrogels; lab-on-a-chip devices; labeling