title: Combination of platelet-rich plasma within periodontal ligament stem cell sheets enhances cell differentiation and matrix production.
abstract: The longstanding goal of periodontal therapy is to regenerate periodontal tissues. Although platelet-rich plasma (PRP) has been gaining increasing popularity for use in the orofacial region, whether PRP is useful for periodontal regeneration is still unknown. The purpose of this study was to determine whether a mixture of periodontal ligament stem cell (PDLSC) sheets and PRP promoted bone regeneration, one of the most important measurement indices of periodontal tissue regenerative capability in vitro and in vivo. In this study, we evaluated the effects of different doses of PRP on the differentiation of human PDLSCs. Then cell sheet formation, extracellular matrix deposition and osteogenic gene expression in response to different doses of PRP treatment during sheet grafting was investigated. Furthermore, we implanted PDLSC sheets treated with 1% PRP subcutaneously into immunocompromised mice to evaluate their bone-regenerative capability. The results revealed that 1% PRP significantly enhanced the osteogenic differentiation of PDLSCs. Based on the production of extracellular matrix proteins, the results of scanning electron microscopy and the expression of the osteogenic genes ALP, Runx2, Col-1 and OCN, the provision of 1% PRP for PDLSC sheets was the most effective PRP administration mode for cell sheet formation. The results of in vivo transplantation showed that 1% PRP-mediated PDLSC sheets exhibited better periodontal tissue regenerative capability than those obtained without PRP intervention. These data suggest that a suitable concentration of PRP stimulation may enhance extracellular matrix production and positively affect cell behaviour in PDLSC sheets. Copyright © 2014 John Wiley & Sons, Ltd.
authors.affiliation: State Key Laboratory of Military Stomatology, Centre for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.|Department of Periodontology, School of Stomatology, Zunyi Medical Collage, Guizhou, People's Republic of China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Military Stomatology, Centre for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.|Research and Development Centre for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.
authors.forename: Bei
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Stomatology, First Affiliated Hospital, Guangzhou Medical University, Guangdong, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Department of Oral and Maxillofacial Surgery, General Hospital of Shenyang Military Area Command, Liaoning, People's Republic of China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Military Stomatology, Centre for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Military Stomatology, Centre for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.|Department of Stomatology, First Affiliated Hospital, Guangzhou Medical University, Guangdong, People's Republic of China.
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Military Stomatology, Centre for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.|Department of Stomatology, First Affiliated Hospital, Guangzhou Medical University, Guangdong, People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Periodontology, School of Stomatology, Zunyi Medical Collage, Guizhou, People's Republic of China.
authors.forename: Song
authors.initials: S
authors.lastname: Ge
authors.affiliation: State Key Laboratory of Military Stomatology, Centre for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.|Research and Development Centre for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000293:Adolescent; D000818:Animals; D002454:Cell Differentiation; D002469:Cell Separation; D002648:Child; D003094:Collagen / Q000096:biosynthesis; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D008819:Mice, Nude; D010012:Osteogenesis; D010513:Periodontal Ligament / Q000166:cytology*; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology*; D055815:Young Adult
chemical_list: D003094:Collagen
keywords: bone regeneration; cell sheet; osteogenic differentiation; periodontal ligament stem cells; periodontal regeneration; platelet-rich plasma