title: Overexpression of MLN51 triggers P-body disassembly and formation of a new type of RNA granules.
abstract: Metastatic lymph node 51 (MLN51, also known as CASC3) is a core component of the exon junction complex (EJC), which is loaded onto spliced mRNAs and plays an essential role in determining their fate. Unlike the three other EJC core components [eIF4AIII, Magoh and Y14 (also known as RBM8A)], MLN51 is mainly located in the cytoplasm, where it plays a key role in the assembly of stress granules. In this study, we further investigated the cytoplasmic role of MLN51. We show that MLN51 is a new component of processing bodies (P-bodies). When overexpressed, MLN51 localizes in novel small cytoplasmic foci. These contain RNA, show directed movements and are distinct from stress granules and P-bodies. The appearance of these foci correlates with the process of P-body disassembly. A similar reduction in P-body count is also observed in human HER2-positive (HER2(+)) breast cancer cells overexpressing MLN51. This suggests that P-body disassembly and subsequent mRNA deregulation might correlate with cancer progression.
authors.affiliation: Université de Rennes 1, UMR CNRS 6290 IGDR, «Translation and Folding Team», Campus de Beaulieu, 35042 Rennes cedex, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Cougot
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, CNRS/U964 INSERM/Université de Strasbourg, 67404 Illkirch, France.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Daguenet
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, CNRS/U964 INSERM/Université de Strasbourg, 67404 Illkirch, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Baguet
authors.affiliation: Université de Rennes 1, UMR CNRS 6290 IGDR, «Translation and Folding Team», Campus de Beaulieu, 35042 Rennes cedex, France.
authors.forename: Annie
authors.initials: A
authors.lastname: Cavalier
authors.affiliation: Université de Rennes 1, UMR CNRS 6290 IGDR, «Translation and Folding Team», Campus de Beaulieu, 35042 Rennes cedex, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Thomas
authors.affiliation: Unité INSERM 991, Plateforme histopathologique, IFR 140 GFAS, Université de Rennes 1, 35043 Rennes, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Bellaud
authors.affiliation: Unité INSERM 991, Plateforme histopathologique, IFR 140 GFAS, Université de Rennes 1, 35043 Rennes, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Fautrel
authors.affiliation: Centre de Ressources Biologiques Santé de Rennes, Centre Eugène Marquis, Rue de la Bataille Flandres Dunkerque - 35042 Rennes cedex, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Godey
authors.affiliation: Institut de Génétique Moléculaire de Montpellier, CNRS, UMR 5535, 34293 Montpellier cedex 5, France edouard.bertrand@igmm.cnrs.fr cat@igbmc.fr reynald.gillet@univ-rennes1.fr.
authors.forename: Edouard
authors.initials: E
authors.lastname: Bertrand
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, CNRS/U964 INSERM/Université de Strasbourg, 67404 Illkirch, France edouard.bertrand@igmm.cnrs.fr cat@igbmc.fr reynald.gillet@univ-rennes1.fr.
authors.forename: Catherine
authors.initials: C
authors.lastname: Tomasetto
authors.affiliation: Université de Rennes 1, UMR CNRS 6290 IGDR, «Translation and Folding Team», Campus de Beaulieu, 35042 Rennes cedex, France edouard.bertrand@igmm.cnrs.fr cat@igbmc.fr reynald.gillet@univ-rennes1.fr.
authors.forename: Reynald
authors.initials: R
authors.lastname: Gillet
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000235:genetics / Q000378:metabolism*; D039601:Eukaryotic Initiation Factor-4A / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C459802:CASC3 protein, human; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; C406243:RBM8A protein, human; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D039601:Eukaryotic Initiation Factor-4A
keywords: Breast cancer; MLN51; P-bodies; Stress granules; mRNA