title: The association between CYP1A1 genetic polymorphisms and coronary artery disease in the Uygur and Han of China.
abstract: BACKGROUND
  The cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1) gene is expressed in the vascular endothelium, which metabolizes arachidonic acid into 20-hydroxyeicosatetraenoic acid (20-HETE) and epoxyeicosatrienoic acids (EETs). 20-HETE mediates cardiovascular homeostasis and growth response in vascular smooth muscle cells (VSMCs) as well as the anti-platelet effect. EETs are potent endogenous vasodilators and inhibitors of vascular inflammation. This study assessed the association between human CYP1A1 gene polymorphisms and coronary artery disease (CAD) in the Uygur and Han in China.
  
  
  METHODS
  Two independent case-control studies that recruited Han (389 patients with CAD and 411 controls) and Uygur participants (293 patients with CAD and 408 controls) analyzed the relationship between CYP1A1 single nucleotide polymorphisms (SNPs: rs4886605, rs12441817, rs4646422 and rs1048943) and CAD. All patients with CAD and controls were genotyped for the four SNPs of CYP1A1 using TaqMan SNP genotyping assays.
  
  
  RESULTS
  In the Uygur group, the distribution of the dominant model(CC vs CT + TT) of rs4886605 for the total sample and the males was significantly different between CAD patients and control participants (P = 0.001 and P = 0.012, respectively), The difference remained significant after a multivariate adjustment (P = 0.018, P = 0.015, respectively). The rs12441817 was also associated with CAD in a dominant model for all participants (P = 0.003) and men (P = 0.012), and the difference remained significant after a multivariate adjustment (P = 0.016, P = 0.002, respectively). However, we did not observe differences in the Uygur females and Han group with regard to the allele frequency or genotypic distribution of rs4886605 and rs12441817 between patients with CAD and control participants. Patients with CAD did not significantly differ from the control participants with regard to the distributions of rs4646422 and rs1048943 genotypes, the dominant model, the recessive model, or allele frequency in the Han and Uygur groups.
  
  
  CONCLUSIONS
  Both rs4886605 and rs12441817 SNPs of the CYP1A1 gene are associated with CAD in the Uygur population of China.
authors.forename: Jin-Guo
authors.initials: JG
authors.lastname: Zou
authors.affiliation: Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, People's Republic of China. myt_xj@sina.com.
authors.forename: Yi-Tong
authors.initials: YT
authors.lastname: Ma
authors.forename: Xiang
authors.initials: X
authors.lastname: Xie
authors.forename: Yi-Ning
authors.initials: YN
authors.lastname: Yang
authors.forename: Shuo
authors.initials: S
authors.lastname: Pan
authors.forename: Dilare
authors.initials: D
authors.lastname: Adi
authors.forename: Fen
authors.initials: F
authors.lastname: Liu
authors.forename: Bang-Dang
authors.initials: BD
authors.lastname: Chen
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D002681:China; D003324:Coronary Artery Disease / Q000201:enzymology / Q000235:genetics*; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: C569463:CYP1A1 protein, human; D019363:Cytochrome P-450 CYP1A1