title: Protection from infection with influenza A H7N9 virus in a mouse model by equine neutralizing F(ab')2.
abstract: Influenza A H7N9 virus has demonstrated considerable pandemic potential in China ever since early spring 2013. Until now, there have been no specific medicines to treat influenza A H7N9 virus infected patients. Development of a safe and effective H7N9 therapeutic preparation is urgently needed. To this end, we prepared and evaluated the pepsin-digested F(ab')2 fragments of serum IgGs from the horses inoculated with a inactivated influenza A H7N9 whole virus antigens. The protective effects of the F(ab')2 fragments against H7N9 virus infection were determined in cultured MDCK cells by cytopathic effect (CPE) and evaluated in a BALB/c mouse model by observing death, weight loss and viral load. The in vitro results showed that the F(ab')2 fragments had an HI titer of 1:2048 and a neutralization titer of 1: 31,623. The in vivo assays suggested that 600 U of the preparations could efficiently protect BALB/c mice from a lethal dose of A/Anhui/01/2013 (H7N9) infection even when administered two days post infection. Thus, this highly purified preparation should be a potential candidate for treating severe patients suffering from influenza A H7N9.
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
authors.forename: Zhongpeng
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Hematology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors.forename: Chuanbo
authors.initials: C
authors.lastname: Fan
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
authors.forename: Yueqiang
authors.initials: Y
authors.lastname: Duan
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
authors.forename: Liangyan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Laboratory Animal Center, Academy of Military Medical Science, Beijing 100071, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Yang
authors.affiliation: Beijing 302 Hospital, Beijing 100039, China.
authors.forename: Ruisheng
authors.initials: R
authors.lastname: Li
authors.affiliation: Beijing 302 Hospital, Beijing 100039, China.
authors.forename: Penghui
authors.initials: P
authors.lastname: Yang
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China. Electronic address: xiaozzp@hotmail.com.
authors.forename: Xiliang
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004285:Dogs; D006736:Horses; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000008:administration & dosage*; D007167:Immunotherapy / Q000379:methods*; D064766:Influenza A Virus, H7N9 Subtype / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology / Q000628:therapy*; D061985:Madin Darby Canine Kidney Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000628:therapy*; D019562:Viral Load / Q000187:drug effects
chemical_list: D057134:Antibodies, Neutralizing; D007140:Immunoglobulin Fab Fragments
keywords: Equine immunoglobulin F(ab′)(2) fragments;; H7N9; Mice