title: Microsatellite markers for Cariniana legalis (Lecythidaceae) and their transferability to C. estrellensis.
abstract: OBJECTIVE
  Microsatellite primers were developed in the neotropical tree species Cariniana legalis (Lecythidaceae) to investigate its genetic diversity, mating system, and gene flow. •
  
  
  RESULTS
  We identified 96 clones containing 82 repeat motifs from a genomic library enriched for (CT)8 and (GT)8 motifs. Primer pairs were developed for 13 microsatellite loci and validated in 51 C. legalis specimens and 26 C. estrellensis specimens. Eleven loci were polymorphic, revealing a maximum of two to 15 alleles per locus in C. legalis and three to 12 in C. estrellensis. For C. legalis, the observed (H o) and expected (H e) heterozygosities ranged from 0 to 0.99 and from 0.07 to 0.90, respectively. For C. estrellensis, H o and H e ranged from 0 to 0.96 and from 0.14 to 0.91, respectively. •
  
  
  CONCLUSIONS
  The primers identified polymorphic loci that are suitable to study genetic diversity and structure, mating system, and gene flow in C. legalis and the related species C. estrellensis.
authors.affiliation: Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Av. Pádua Dias 11, CP 9, Piracicaba, São Paulo 13418-900, Brazil.
authors.forename: Evandro Vagner
authors.initials: EV
authors.lastname: Tambarussi
authors.affiliation: Instituto Florestal de São Paulo, CP 1322, São Paulo, São Paulo 01059-970, Brazil.
authors.forename: Alexandre Magno
authors.initials: AM
authors.lastname: Sebbenn
authors.affiliation: Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Av. Pádua Dias 11, CP 9, Piracicaba, São Paulo 13418-900, Brazil.
authors.forename: Maria Andréia
authors.initials: MA
authors.lastname: Moreno
authors.affiliation: Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Av. Pádua Dias 11, CP 9, Piracicaba, São Paulo 13418-900, Brazil.
authors.forename: Elza Martins
authors.initials: EM
authors.lastname: Ferraz
authors.affiliation: Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Av. Pádua Dias 11, CP 9, Piracicaba, São Paulo 13418-900, Brazil.
authors.forename: Paulo Yoshio
authors.initials: PY
authors.lastname: Kageyama
authors.affiliation: Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Av. Pádua Dias 11, CP 9, Piracicaba, São Paulo 13418-900, Brazil.
authors.forename: Roland
authors.initials: R
authors.lastname: Vencovsky
keywords: Brazilian Atlantic forest; Cariniana legalis; conservation genetics; microsatellite markers; population genetics; tropical tree species