title: Evaluation of the novel microarray-based Prove-it™ Bone&Joint assay for direct detection of pathogens from normally sterile body sites in comparison with culture and broad-range bacterial PCR.
abstract: The performance of Prove-it™ Bone&Joint, a novel microarray-based assay for direct pathogen detection from clinical samples was assessed. In culture-positive samples, Prove-it™ performed similarly with broad-range bacterial PCR in osteoarticular (sensitivity 62.9% vs. 57.7%) and non-osteoarticular samples (54.6% vs. 56.8%). Prove-it™ is a rapid tool providing preliminary results while awaiting culture results.
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland. Electronic address: vladimira.hinic@usb.ch.
authors.forename: Vladimira
authors.initials: V
authors.lastname: Hinić
authors.affiliation: Mobidiag Ltd., Helsinki, Finland.
authors.forename: Anne
authors.initials: A
authors.lastname: Aittakorpi
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Suter
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland.
authors.forename: Selda
authors.initials: S
authors.lastname: Turan
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Schultheiss
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland.
authors.forename: Reno
authors.initials: R
authors.lastname: Frei
authors.affiliation: Division of Clinical Microbiology, University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Goldenberger
mesh_terms: D001170:Arthritis, Infectious / Q000175:diagnosis* / Q000382:microbiology*; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D010000:Osteitis / Q000175:diagnosis* / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
keywords: Broad-range bacterial PCR; Culture; Direct molecular detection; Microarray; Osteoarticular and other specimens