title: RP-HPLC characterization of lupenone and β-sitosterol in rhizoma musae and evaluation of the anti-diabetic activity of lupenone in diabetic Sprague-Dawley rats.
abstract: With the aim of characterizing the active ingredients lupenone and β-sitosterol in Rhizoma Musae samples a reversed-phase HPLC method for the separation of these two compounds in Rhizoma Musae samples was developed (regression coefficient>0.9996). The method was further applied to quantify lupenone and β-sitosterol content in Rhizoma Musae samples cultured in different growth environments. Different variables such as geographical location, growth stage, and harvest time, demonstrated differential effects on lupenone and β-sitosterol levels. Moreover, we determined the optimum conditions for cultivation and harvesting of Rhizoma Musae herbs. Lupenone administration caused a significant reduction in fasting blood glucose (FBG) levels in diabetic rats at doses of 1.78, 5.33, and 16.00 mg·kg⁻¹·day⁻¹ for 14 days, the glycated hemoglobin (HbA1c) levels of diabetic rats also significantly reduced at doses of 5.33, and 16.00 mg·kg⁻¹·day⁻¹, indicating a robust antidiabetic activity. To our knowledge, this is the first report of an optimized HPLC method successfully applied to quantify lupenone and β-sitosterol, and its applicability in optimizing Rhizoma Musae growth. Animal experiments also showed for the first time that lupenone from Rhizoma Musae has anti-diabetic activity.
authors.affiliation: Department of Pharmacognosy, Guiyang College of Traditional Chinese Medicine, 50, Nanming District, Guiyang 550002, Guizhou, China. xf333666999@sina.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Pharmacognosy, Guiyang College of Traditional Chinese Medicine, 50, Nanming District, Guiyang 550002, Guizhou, China. whm0425@126.com.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Pharmacognosy, Guiyang College of Traditional Chinese Medicine, 50, Nanming District, Guiyang 550002, Guizhou, China. wxp0123@126.com.
authors.forename: Xiangpei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pharmacognosy, Guiyang College of Traditional Chinese Medicine, 50, Nanming District, Guiyang 550002, Guizhou, China. yangye459@163.com.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Pharmacognosy, Guiyang College of Traditional Chinese Medicine, 50, Nanming District, Guiyang 550002, Guizhou, China. wym01234@sina.com.
authors.forename: Yuanmin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pharmacognosy, Guiyang College of Traditional Chinese Medicine, 50, Nanming District, Guiyang 550002, Guizhou, China. qianhaibing888@163.com.
authors.forename: Haibing
authors.initials: H
authors.lastname: Qian
authors.affiliation: Department of Pharmacognosy, Guiyang College of Traditional Chinese Medicine, 50, Nanming District, Guiyang 550002, Guizhou, China. zhangyanyan111@sina.com.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D004353:Drug Evaluation, Preclinical; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D057230:Limit of Detection; D008297:Male; D028521:Musa / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D027343:Rhizome / Q000737:chemistry*; D012855:Sitosterols / Q000302:isolation & purification / Q000494:pharmacology*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D000094042:Lupanes
chemical_list: D007004:Hypoglycemic Agents; D010936:Plant Extracts; D012855:Sitosterols; D014315:Triterpenes; C470592:lupenone; D000094042:Lupanes; C025473:gamma-sitosterol