title: Simple and rapid CD4 testing based on large-field imaging system composed of microcavity array and two-dimensional photosensor.
abstract: This study presents a novel method for CD4 testing based on one-shot large-field imaging. The large-field imaging system was fabricated by a microcavity array and a two-dimensional (2D) photosensor within the desk-top-sized instrument. The microcavity array was employed to separate leukocytes from whole blood based on differences in the size of leukocytes and other blood cells. The large-field imaging system with lower side irradiation enabled acquisition of cell signatures with high signal-to-noise ratio, because the metallic substrate of the microcavity array obstructed excessive excitation light. In this setting, dual-color imaging of CD4(+) and CD8(+) T cells was achieved within the entire image area (64 mm(2)) in 2s. The practical performance of the large-field imaging system was demonstrated by determining the CD4/CD8 ratio in a few microliter of control whole blood as small as those obtained by a finger prick. The CD4/CD8 ratios measured using the large-field imaging system correlated well with those measured by microscopic analysis. These results indicate that our proposed system provides a simple and rapid CD4 testing for the application of HIV/AIDS treatment.
authors.affiliation: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Saeki
authors.affiliation: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Sugamura
authors.affiliation: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Masahito
authors.initials: M
authors.lastname: Hosokawa
authors.affiliation: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yoshino
authors.affiliation: Malcom Co., Ltd., 4-15-10, Honmachi, Shibuya-ku, Tokyo 151-0071, Japan.
authors.forename: Tae-Kyu
authors.initials: TK
authors.lastname: Lim
authors.affiliation: Malcom Co., Ltd., 4-15-10, Honmachi, Shibuya-ku, Tokyo 151-0071, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Harada
authors.affiliation: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Matsunaga
authors.affiliation: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan. Electronic address: tsuyo@cc.tuat.ac.jp.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000628:therapy; D015374:Biosensing Techniques; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology*; D018414:CD8-Positive T-Lymphocytes / Q000473:pathology*; D002469:Cell Separation; D006801:Humans; D046210:Microfluidic Analytical Techniques; D059629:Signal-To-Noise Ratio; D046888:Tissue Array Analysis / Q000379:methods
keywords: 2D photosensor; CD4 testing; HIV/AIDS treatment; Large-field imaging system; Microcavity array