title: CUL4B promotes proliferation and inhibits apoptosis of human osteosarcoma cells.
abstract: Cullin 4B (CUL4B) is a component of the Cullin4B-Ring E3 ligase complex (CRL4B) that functions in proteolysis and is implicated in tumorigenesis. Here, we report that CUL4B is associated with tumorigenesis by promoting proliferation and inhibiting apoptosis of human osteosarcoma cells. We performed RNA interference (RNAi) with a lentiviral vector system to silence the CUL4B gene using osteosarcoma SAOS-2 cells. The negative control included the normal target cells infected with the negative control virus whereas the knockdown cells included the normal target cells transfected with the RNAi target virus. We assessed the inhibition resulting from the decreased expression of the CUL4B gene on the proliferation rate of SAOS-2 cells, and also evaluated the cell cycle distribution, apoptosis and clonability. Compared with the negative control, the CUL4B gene expression was significantly inhibited in the SAOS-2 cells at the mRNA and protein levels in the knockdown group (P<0.01). Furthermore, in the knockdown group, the cell proliferation rate and clonability were also significantly inhibited (P<0.01). The apoptosis rate increased significantly (P<0.05). A significant decrease in the number of cells in the G1 phase (P<0.01) and significant increases in the S (P<0.01) and G2 phases (P<0.05) were observed. The silencing of CUL4B gene expression can effectively inhibit osteosarcoma cell proliferation and induce apoptosis. These findings may provide a novel biomarker for the treatment of osteosarcoma.
authors.affiliation: Department of Orthopedics, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, Shanghai Jiading Central Hospital, Shanghai 201800, P.R. China.
authors.forename: Bao-Liang
authors.initials: BL
authors.lastname: Shen
authors.affiliation: Department of Emergency and Trauma, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China.
authors.forename: Qing-Ge
authors.initials: QG
authors.lastname: Fu
authors.affiliation: Department of Orthopedics, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Yi-Xing
authors.initials: YX
authors.lastname: Tang
authors.affiliation: Department of Orthopedics, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Cang-Long
authors.initials: CL
authors.lastname: Hou
authors.affiliation: Department of Orthopedics, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
mesh_terms: D017209:Apoptosis; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D044842:Cullin Proteins / Q000235:genetics* / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D006801:Humans; D008954:Models, Biological; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D034622:RNA Interference
chemical_list: C517832:CUL4B protein, human; D044842:Cullin Proteins