title: Thermal stress induces changes in gene expression and blood parameters in high and low feed efficiency meat quail.
abstract: In this study, we analysed markers of stress, plasma creatinine and T3 content, and insulin-like growth factor I (IGF-I), growth hormone receptor (GHR), uncoupling protein (UCP), adenine nucleotide translocase (ANT) and cytochrome c oxidase subunit III (COX III) mRNA expression in the liver and muscle of high (0.22 g/g) and low (0.14 g/g) feed efficiency (FE) meat quail at three different air temperatures, comfortable, heat and cold stress, for 24 h. High FE quail presented higher plasma T3 and lower creatinine levels. IGF-I mRNA expression was higher in the livers of high FE quail than in the livers of low FE quail under both comfortable and cold stress conditions. In the muscle, regardless of the environment, high FE birds showed higher IGF-I mRNA expression. High FE birds also showed higher GHR mRNA expression under comfortable conditions. Regarding the environment, higher expression was observed in birds at comfortable conditions, and lower expression in birds under heat stress. UCP mRNA expression in the liver was lower in high FE birds and higher under heat stress compared with the other conditions. Low and high FE birds showed greater ANT mRNA expression in the muscle under cold stress. Greater mRNA COX III expressions were observed in the liver and muscle of quails under comfortable conditions. Our results suggest that temperature affects the expression of genes related to growth and mitochondrial energy production, and quail with different FEs respond differently to environmental stimuli. In comfortable conditions, high FE animals show higher IGF-I mRNA expression and plasma T3 and lower creatinine content.
authors.affiliation: Department of Animal Science (sponsored by CNPq), State University of Maringá, av. Colombo, 5790, Maringá, PR, Brazil, egasparino@uem.br.
authors.forename: Eliane
authors.initials: E
authors.lastname: Gasparino
authors.forename: Débora Marques
authors.initials: DM
authors.lastname: Voltolini
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Del Vesco
authors.forename: Simara Marcia
authors.initials: SM
authors.lastname: Marcato
authors.forename: Vittor
authors.initials: V
authors.lastname: Zancanela
authors.forename: Daiane
authors.initials: D
authors.lastname: de Oliveira Grieser
authors.forename: Angélica
authors.initials: A
authors.lastname: de Souza Khatlab
authors.forename: Simone Eliza Facioni
authors.initials: SE
authors.lastname: Guimarães
authors.forename: Adhemar Rodriges
authors.initials: AR
authors.lastname: de Oliveira Neto
mesh_terms: D000821:Animal Feed; D000818:Animals; D003404:Creatinine / Q000097:blood; D003576:Electron Transport Complex IV / Q000235:genetics; D015870:Gene Expression; D018869:Heat-Shock Response; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D007473:Ion Channels / Q000235:genetics; D008099:Liver / Q000378:metabolism; D008460:Meat; D000226:Mitochondrial ADP, ATP Translocases / Q000235:genetics; D024101:Mitochondrial Proteins / Q000235:genetics; D018482:Muscle, Skeletal / Q000378:metabolism; D011784:Quail / Q000097:blood* / Q000235:genetics*; D011986:Receptors, Somatotropin / Q000235:genetics; D013696:Temperature; D014284:Triiodothyronine / Q000097:blood; D000071256:Uncoupling Protein 1
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; D011986:Receptors, Somatotropin; D000071256:Uncoupling Protein 1; D014284:Triiodothyronine; D007334:Insulin-Like Growth Factor I; D000226:Mitochondrial ADP, ATP Translocases; D003404:Creatinine; D003576:Electron Transport Complex IV