title: Kinetic approach for the purification of nucleotides with magnetic separation.
abstract: The isolation of β-nicotinamide adenine dinucleotide is of great importance since it is widely used in different scientific and technologic fields such as biofuel cells, sensor technology, and hydrogen production. In order to isolate β-nicotinamide adenine dinucleotide, first 3-aminophenyboronic acid functionalized magnetic nanoparticles were prepared to serve as a magnetic solid support and subsequently they were used for reversible adsorption/desorption of β-nicotinamide adenine dinucleotide in a batch fashion. The loading capacity of the 3-aminophenyboronic acid functionalized nanoparticles for β-nicotinamide adenine dinucleotide adsorption was 13.0 μmol/g. Adsorption kinetic and isotherm studies showed that the adsorption process followed a pseudo-second-order kinetic model and the experimental data can be represented using Langmuir isotherm model. The 3-aminophenyboronic acid functionalized magnetic nanoparticles were proposed as an alternative support for the β-nicotinamide adenine dinucleotide purification. The results elucidated the significance of magnetic separation as a fast, relatively simple, and low-cost technique. Furthermore, the magnetic supports can be reused at least five times for purification processes.
authors.affiliation: Department of Chemistry, Faculty of Education, Dicle University, Diyarbakir, Turkey.
authors.forename: Servet
authors.initials: S
authors.lastname: Tural
authors.forename: Bilsen
authors.initials: B
authors.lastname: Tural
authors.forename: Mehmet Şakir
authors.initials: MŞ
authors.lastname: Ece
authors.forename: Evren
authors.initials: E
authors.lastname: Yetkin
authors.forename: Necati
authors.initials: N
authors.lastname: Özkan
mesh_terms: D000327:Adsorption; D056804:Biofuels; D001897:Boronic Acids / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008024:Ligands; D008280:Magnetics; D009243:NAD / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D044367:Phase Transition; D015203:Reproducibility of Results; D012833:Siloxanes / Q000737:chemistry
chemical_list: D056804:Biofuels; D001897:Boronic Acids; D008024:Ligands; D012833:Siloxanes; D009243:NAD; C028592:3-aminobenzeneboronic acid; D006859:Hydrogen
keywords: Adsorption; Desorption; Magnetic assisted separation; Nucleotides; Reusability