title: Drosophila Yemanuclein associates with the cohesin and synaptonemal complexes.
abstract: Meiosis is characterized by two chromosome segregation rounds (meiosis I and II), which follow a single round of DNA replication, resulting in haploid genome formation. Chromosome reduction occurs at meiosis I. It relies on key structures, such as chiasmata, which are formed by repair of double-strand breaks (DSBs) between the homologous chromatids. In turn, to allow for segregation of homologs, chiasmata rely on the maintenance of sister chromatid cohesion. In most species, chiasma formation requires the prior synapsis of homologous chromosome axes, which is mediated by the synaptonemal complex, a tripartite proteinaceous structure specific to prophase I of meiosis. Yemanuclein (Yem) is a maternal factor that is crucial for sexual reproduction. It is required in the zygote for chromatin assembly of the male pronucleus, where it acts as a histone H3.3 chaperone in complex with Hira. We report here that Yem associates with the synaptonemal complex and the cohesin complex. A genetic interaction between yem(1) (V478E) and the Spo11 homolog mei-W68, modified a yem(1) dominant effect on crossover distribution, suggesting that Yem has an early role in meiotic recombination. This is further supported by the impact of yem mutations on DSB kinetics. A Hira mutation gave a similar effect, presumably through disruption of Hira-Yem complex.
authors.affiliation: Institut de Génétique Humaine, Unité Propre de Recherche 1142, Centre National de la Recherche Scientifique (CNRS), 34396 Montpellier, France.
authors.forename: Régis E
authors.initials: RE
authors.lastname: Meyer
authors.affiliation: Institut de Génétique Humaine, Unité Propre de Recherche 1142, Centre National de la Recherche Scientifique (CNRS), 34396 Montpellier, France.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Algazeery
authors.affiliation: Institut de Génétique Humaine, Unité Propre de Recherche 1142, Centre National de la Recherche Scientifique (CNRS), 34396 Montpellier, France.
authors.forename: Michèle
authors.initials: M
authors.lastname: Capri
authors.affiliation: Institut de Génétique Humaine, Unité Propre de Recherche 1142, Centre National de la Recherche Scientifique (CNRS), 34396 Montpellier, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Brazier
authors.affiliation: Institut de Génétique Humaine, Unité Propre de Recherche 1142, Centre National de la Recherche Scientifique (CNRS), 34396 Montpellier, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Ferry
authors.affiliation: Institut de Génétique Humaine, Unité Propre de Recherche 1142, Centre National de la Recherche Scientifique (CNRS), 34396 Montpellier, France Institute of Regenerative Medicine and Biotherapy (IRMB), INSERM U1040/Hôpital Saint-Eloi CHRU, 34295 Montpellier, France ounissa.ait-ahmed@inserm.fr.
authors.forename: Ounissa
authors.initials: O
authors.lastname: Aït-Ahmed
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D053903:DNA Breaks, Double-Stranded; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism*; D005260:Female; D008297:Male; D008540:Meiosis; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D013573:Synaptonemal Complex / Q000378:metabolism*; D000097722:Cohesins
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009687:Nuclear Proteins; D000097722:Cohesins; C075160:yem protein, Drosophila
keywords: Cohesin; Drosophila; Hira; Meiosis; Oocyte; Recombination; Synaptonemal complex; Yem; Yemanuclein