title: Development and characterization of 37 novel EST-SSR markers in Pisum sativum (Fabaceae).
abstract: OBJECTIVE
  Simple sequence repeat markers were developed based on expressed sequence tags (EST-SSR) and screened for polymorphism among 23 Pisum sativum individuals to assist development and refinement of pea linkage maps. In particular, the SSR markers were developed to assist in mapping of white mold disease resistance quantitative trait loci. •
  
  
  RESULTS
  Primer pairs were designed for 46 SSRs identified in EST contiguous sequences assembled from a 454 pyrosequenced transcriptome of the pea cultivar, 'LIFTER'. Thirty-seven SSR markers amplified PCR products, of which 11 (30%) SSR markers produced polymorphism in 23 individuals, including parents of recombinant inbred lines, with two to four alleles. The observed and expected heterozygosities ranged from 0 to 0.43 and from 0.31 to 0.83, respectively. •
  
  
  CONCLUSIONS
  These EST-SSR markers for pea will be useful for refinement of pea linkage maps, and will likely be useful for comparative mapping of pea and as tools for marker-based pea breeding.
authors.affiliation: Department of Plant, Soil and Microbial Sciences, Michigan State University, 107 CIPS, East Lansing, Michigan 48824 USA.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zhuang
authors.affiliation: Department of Plant Sciences, North Dakota State University, 370G Loftsgard Hall, Fargo, North Dakota 58108 USA.
authors.forename: Kevin E
authors.initials: KE
authors.lastname: McPhee
authors.affiliation: Dow AgroSciences LLC, 9330 Zionsville Road, Indianapolis, Indiana 46268 USA.
authors.forename: Tristan E
authors.initials: TE
authors.lastname: Coram
authors.affiliation: Department of Plant Pathology, Washington State University, Pullman, Washington 99164 USA.
authors.forename: Tobin L
authors.initials: TL
authors.lastname: Peever
authors.affiliation: Department of Plant, Soil and Microbial Sciences, Michigan State University, 107 CIPS, East Lansing, Michigan 48824 USA.
authors.forename: Martin I
authors.initials: MI
authors.lastname: Chilvers
keywords: EST-SSR; Fabaceae; Pisum sativum; Sclerotinia sclerotiorum; microsatellite; transcriptome