title: Nanotherapy with hybrid liposomes for colorectal cancer along with apoptosis in vitro and in vivo.
abstract: OBJECTIVE
  We examined the therapeutic effects of hybrid liposomes (HL) composed of L-α-dimyristylphosphati-dylcholine (DMPC) and polyoxyethylene (25) dodecyl ether (C12(EO)25) on the growth of human colorectal cancer (WiDr) cells in vitro and in vivo.
  
  
  METHODS
  HL composed of 95 mol% DMPC and 5 mol% C12(EO)25 were prepared by the sonication method and their therapeutic effects in xenograft mouse models of colorectal cancer liver metastases were examined in vivo.
  
  
  RESULTS
  The inhibitory effects of HL-25 on the growth of WiDr cells along with apoptosis were assessed in vitro. Remarkable inhibitory effects of HL-25 for the liver metastasis of colorectal cancer cells along with apoptosis were revealed on the basis of histological analysis. Prolonged survival was attained for the xenograft mouse model of colorectal cancer after treatment with HL-25 in vivo.
  
  
  CONCLUSIONS
  Therapeutic effects of HL-25 without any drugs on the liver metastasis of human colorectal cancer were obtained for the first time in vivo.
authors.affiliation: Division of Applied Life Science, Graduate School of Engineering, Sojo University, Kumamoto, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ichihara
authors.affiliation: Immuno-medical Lab, Omote Sando Yoshida Hospital, Kumamoto, Japan.
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Nakagawa
authors.affiliation: Immuno-medical Lab, Omote Sando Yoshida Hospital, Kumamoto, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Matsuoka
authors.affiliation: Immuno-medical Lab, Omote Sando Yoshida Hospital, Kumamoto, Japan.
authors.forename: Kenshi
authors.initials: K
authors.lastname: Yoshida
authors.affiliation: Division of Applied Life Science, Graduate School of Engineering, Sojo University, Kumamoto, Japan matumoto@life.sojo-u.ac.jp.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Matsumoto
authors.affiliation: Division of Applied Life Science, Graduate School of Engineering, Sojo University, Kumamoto, Japan Immuno-medical Lab, Omote Sando Yoshida Hospital, Kumamoto, Japan.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Ueoka
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000188:drug therapy / Q000473:pathology / Q000556:secondary; D051379:Mice; D050997:Nanomedicine; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D047368:Tumor Burden / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D008081:Liposomes; C558492:polyoxyethylene(25)dodecyl ether; D011092:Polyethylene Glycols; D004134:Dimyristoylphosphatidylcholine
keywords: Hybrid liposome; apoptosis; colorectal cancer; liver metastasis; nanotherapy