title: The growth-promoting activity of egg white proteins in the C2C12 myoblast cell line.
abstract: In this study, we examined the effects of several egg white proteins (ovalbumin, ovomucoid, ovotransferrin and lysozyme) on proliferation and myotube growth in C2C12 murine myoblast cells. Cell proliferation was measured using a water-soluble tetrazolium salt (WST-8)-based assay and then validated using Giemsa staining. Significant proliferative activities of C2C12 cells were observed in response to the addition of 10(-5) -10(-4) mol/L ovalbumin or ovomucoid. Ovotransferrin decreased C2C12 cell proliferation and lysozyme showed no significant effects on the proliferation of C2C12 cells. In contrast, the proliferative effects of ovalbumin and ovomucoid were not observed in 3T3-L1 murine preadipocyte cells. We also measured the effects of ovalbumin and ovomucoid on C2C12 myotube diameters by using histological analysis. In comparison to control cells, myotube diameters were significantly increased in cells cultured in 10(-6) -10(-4) mol/L ovalbumin or ovomucoid, suggesting that ovalbumin and ovomucoid stimulate the growth of myotubes. Thus, our results clearly demonstrated that ovalbumin or ovomucoid stimulated the proliferation of myoblasts and growth of myotubes.
authors.affiliation: Department of Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Mizunoya
authors.forename: Ayumi
authors.initials: A
authors.lastname: Tashima
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sato
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Tatsumi
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Ikeuchi
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D048708:Cell Growth Processes / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D003207:Conalbumin / Q000494:pharmacology; D004527:Egg Proteins / Q000494:pharmacology*; D051379:Mice; D009113:Muramidase / Q000494:pharmacology; D018485:Muscle Fibers, Skeletal / Q000166:cytology; D032446:Myoblasts / Q000166:cytology*; D010047:Ovalbumin / Q000494:pharmacology; D010059:Ovomucin / Q000494:pharmacology; D013268:Stimulation, Chemical
chemical_list: D004527:Egg Proteins; D003207:Conalbumin; D010059:Ovomucin; D010047:Ovalbumin; D009113:Muramidase
keywords: C2C12; egg white; ovalbumin; ovomucoid; skeletal muscle