title: Molecular diversity of drug resistant mycobacterium tuberculosis strains in Western Turkey.
abstract: OBJECTIVE
  The aim of this study was to investigate the molecular diversity and clonal relationship of drug resistant Mycobacterium tuberculosis strains isolated in Western Turkey.
  
  
  METHODS
  A total of 87 strains isolated between 2006 and 2009, eight of which were rifampicin monoresistant and 79 were multidrug resistant, were analyzed with IS6110 RFLP and spoligotyping methods.
  
  
  RESULTS
  The results of spoligotyping showed that 7% of the strains were orphans, and 8% were undefined for family in the SpolDB4 database. Major families of the strains were LAM (38%), T (35%), Haarlem (7%), Beijing (2%), S (2%) and U (1%) families. The clustering rate by spoligotyping was calculated as 75%. The most predominant SIT cluster was SIT41 (29%). According to the results of IS6110 RFLP, 71 different patterns of IS6110 were observed. Low copy number was found in 26% of the strains. When the results of two methods were combined, the final clustering rate was calculated as 26%.
  
  
  CONCLUSIONS
  The genotypical distribution of drug resistant tuberculosis isolates in our region indicates genetic diversity and the clustering rate was found low in our region. However, more comprehensive and long-term molecular epidemiological studies are needed to control the drug resistant strains.
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Celal Bayar University, Manisa, Turkey.
authors.forename: Süheyla
authors.initials: S
authors.lastname: Sürücüoğlu
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, İnönü University, Malatya, Turkey.
authors.forename: Selami
authors.initials: S
authors.lastname: Günal
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Celal Bayar University, Manisa, Turkey.
authors.forename: Nuri
authors.initials: N
authors.lastname: Ozkütük
authors.affiliation: Department of Medical Microbiology, Dr. Suat Seren Chest Diseases and Surgery Training and Research Hospital, İzmir, Turkey.
authors.forename: Can
authors.initials: C
authors.lastname: Biçmen
authors.affiliation: Department of Tuberculosis and Chest Diseases, Dr. Suat Seren Chest Diseases and Surgery Training and Research Hospital, İzmir, Turkey.
authors.forename: Ayşe
authors.initials: A
authors.lastname: Ozsöz
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Celal Bayar University, Manisa, Turkey.
authors.forename: Hörü
authors.initials: H
authors.lastname: Gazi
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, İnönü University, Malatya, Turkey.
authors.forename: Rıza
authors.initials: R
authors.lastname: Durmaz
keywords: Mycobacterium tuberculosis; drug resistance; epidemiology; genotyping