title: The interaction of N-trifluoroacetylgalactosamine and its derivatives with winged bean (Psophocarpus tetragonolobus) basic agglutinin reveals differential mechanism of their recognition: a fluorine-19 nuclear magnetic resonance study.
abstract: Here, we show the binding results of a leguminosae lectin, winged bean basic agglutinin (WBA I) to N-trifluoroacetylgalactosamine (NTFAGalN), methyl-α-N-trifluoroacetylgalactosamine (MeαNTFAGalN) and methyl-β-tifluoroacetylgalactosamine (MeβNTFAGalN) using (19) F NMR spectroscopy. No chemical shift difference between the free and bound states for NTFAGalN and MeβNTFAGalN, and 0.01-ppm chemical shift change for MeαNTFAGalN, demonstrate that the MeαNTFAGalN has a sufficiently long residence time on the protein binding site as compared to MeβNTFAGalN and the free anomers of NTFAGalN. The sugar anomers were found in slow exchange with the binding site of agglutinin. Consequently, we obtained their binding parameters to the protein using line shape analyses. Aforementioned analyses of the activation parameters for the interactions of these saccharides indicate that the binding of α and β anomers of NTFAGalN and MeαNTFAGalN is controlled enthalpically, while that of MeβNTFAGalN is controlled entropically. This asserts the sterically constrained nature of the interaction of the MeβNTFAGalN with WBA I. These studies thus highlight a significant role of the conformation of the monosaccharide ligands for their recognition by WBA I.
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Bangalore, 560012, India.
authors.forename: Samiksha
authors.initials: S
authors.lastname: Katiyar
authors.forename: Amrita
authors.initials: A
authors.lastname: Singh
authors.forename: Avadhesha
authors.initials: A
authors.lastname: Surolia
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D037121:Plant Lectins / Q000737:chemistry*
chemical_list: D037121:Plant Lectins; C049304:winged bean lectin; D000117:Acetylglucosamine