title: Evaluation of the immunological cellular response of Cebus apella exposed to the carcinogen N-methyl-N-nitrosourea and treated with CANOVA®.
abstract: The immune response modifier Canova® is a homeopathic remedy indicated for patients with depressed immune system, since this drug appears to increase adaptive immunity and induce an immune response against multiple and severe pathological conditions, including cancer. We evaluated the pattern of immune cellular response in non-human primates of the species Cebus apella exposed to N-methyl-N-nitrosourea (MNU) with and without Canova®. Twelve animals were divided into four groups, with three animals each: negative control and three experimental groups, MNU-alone (35 days); MNU (35 days)-plus-Canova® (3 days) and Canova®-alone (3 days). The animals received MNU orally and Canova® by three intravenous injections. Evaluation of the cellular immune response was performed by immunophenotyping of T-lymphocytes (CD4(+), CD8(+)), B-lymphocytes and natural killer cells. Analysis was also performed of the cell cycle. Our results suggest an increase of T-lymphocytes (CD4(+)CD3(+)) only in the Canova® group, while in the MNU-plus-Canova® group only B-lymphocytes increased.
authors.affiliation: Laboratory of Human Cytogenetics, Institute of Biological Sciences, Federal University of Pará, Belém, PA, Brazil daniellefeio@yahoo.com.br.
authors.forename: Danielle Cristinne Azevedo
authors.initials: DC
authors.lastname: Feio
authors.affiliation: National Primate Center, Ministry of Health, Ananindeua, PA, Brazil.
authors.forename: José Augusto Pereira Carneiro
authors.initials: JA
authors.lastname: Muniz
authors.affiliation: Laboratory of Human Cytogenetics, Institute of Biological Sciences, Federal University of Pará, Belém, PA, Brazil.
authors.forename: Raquel Carvalho
authors.initials: RC
authors.lastname: Montenegro
authors.affiliation: Laboratory of Human Cytogenetics, Institute of Biological Sciences, Federal University of Pará, Belém, PA, Brazil.
authors.forename: Rommel Rodriguez
authors.initials: RR
authors.lastname: Burbano
authors.affiliation: Laboratory of General Pathology - Immunopathology and Cytology, Institute of Biological Sciences, Federal University of Pará, Belém, PA, Brazil.
authors.forename: Lacy Cardoso
authors.initials: LC
authors.lastname: De Brito Junior
authors.affiliation: Laboratory of Molecular Biology, Center of Biological and Health Sciences, State University of Pará, Belém, PA, Brazil.
authors.forename: Patrícia Danielle Lima
authors.initials: PD
authors.lastname: De Lima
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D002273:Carcinogens / Q000008:administration & dosage / Q000633:toxicity*; D002428:Cebus; D002453:Cell Cycle / Q000187:drug effects; D003435:Crotalid Venoms / Q000008:administration & dosage / Q000494:pharmacology*; D007111:Immunity, Cellular / Q000187:drug effects* / Q000276:immunology*; D016130:Immunophenotyping; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008770:Methylnitrosourea / Q000008:administration & dosage / Q000633:toxicity*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000954:Antigens, Surface; C513575:Canova medication; D002273:Carcinogens; D003435:Crotalid Venoms; D010936:Plant Extracts; D008770:Methylnitrosourea
keywords: Canova; Cebus apella; MNU; immune response modification; non-human primates