title: Isolation and characterization of microsatellite markers in Beilschmiedia roxburghiana (Lauraceae).
abstract: OBJECTIVE
  Although there are as many as 250 species in the genus Beilschmiedia, their genetic diversity has been poorly investigated. Our objective was to develop microsatellite markers for B. roxburghiana to study its genetic diversity for the sustainable management of this species. •
  
  
  RESULTS
  Using the microsatellite-enriched library and PCR-based screening method, 22 microsatellite markers were developed and 10 showed high polymorphism in a population. The number of alleles per locus for these 10 microsatellites ranged from five to 19. The observed and expected heterozygosities ranged from 0.298 to 1.000 and from 0.314 to 0.878, respectively. •
  
  
  CONCLUSIONS
  Our results from the 10 highly polymorphic microsatellites indicate that the principal reproductive mode of B. roxburghiana is clonal in the studied population. These microsatellites will facilitate further studies on genetic diversity and structure in B. roxburghiana.
authors.affiliation: Laboratory of Plant Phylogenetics and Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan 650223, People's Republic of China ; Graduate University of the Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, People's Republic of China.
authors.forename: Zheng-Feng
authors.initials: ZF
authors.lastname: Wang
authors.affiliation: Laboratory of Plant Phylogenetics and Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan 650223, People's Republic of China.
authors.forename: Qiao-Ming
authors.initials: QM
authors.lastname: Li
authors.affiliation: Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, People's Republic of China.
authors.forename: Wan-Hui
authors.initials: WH
authors.lastname: Ye
authors.affiliation: Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, People's Republic of China.
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Cao
authors.affiliation: Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, People's Republic of China.
authors.forename: Zhang-Ming
authors.initials: ZM
authors.lastname: Wang
keywords: Beilschmiedia roxburghiana; Lauraceae; Xishuangbanna Plot; genetic diversity; microsatellite markers; population genetics