title: Prognostic significance of astrocyte elevated gene-1 in human astrocytomas.
abstract: Astrocyte Elevated Gene-1 (AEG-1) has been proposed as a biomarker for a variety of cancers. This study aimed to investigate the expression of AEG-1 in human astrocytomas and the correlation between AEG-1 expression and clinicopathologic variables of astrocytomas. AEG-1 expression in four pairs of matched astrocytomas tissues and 5 cell lines was detected by immunohistochemical and Western blot analysis. In addition, AEG-1 protein expression was examined by immunohistochemical staining in 204 cases, including 32 normal brain tissues, 80 Low-malignant astrocytomas (LMAs) and 92 High-Malignant astrocytomas (HMAs). AEG-1 expression in 31 LMAs and 29 HMAs samples was detected by RT-PCR and Western blot analysis. We detected AEG-1 expression in normal neurons and glioma cell lines U87, U251 and M059K, but not in normal glial cells. Immunohistochemical analysis showed that 128 of 172 (74.4%) paraffin-embedded archival astrocytomas samples exhibited positive AEG-1 expression. Statistical analysis suggested that higher AEG-1 level was significantly correlated with histological grade of astrocytomas. In addition, AEG-1 mRNA and protein expression was higher in HMAs than in LMAs. AEG-1 expression had no correlation with the gender or age of astrocytoma patients. In summary, our data suggest that AEG-1 may represent a novel prognostic marker for astrocytomas.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital, Harbin Medical University Harbin 150001, China.
authors.forename: Zhangxiu
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Microbiology, Harbin Medical University, Immunity and Infection, Pathogenic Biology Key Laboratory in Heilongjiang Province Harbin 150081, China.
authors.forename: Meihui
authors.initials: M
authors.lastname: He
authors.affiliation: Department of Neurosurgery, The Third Affiliated Hospital, Harbin Medical University Harbin 150001, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital, Harbin Medical University Harbin 150001, China.
authors.forename: Baozhan
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Microbiology, Harbin Medical University, Immunity and Infection, Pathogenic Biology Key Laboratory in Heilongjiang Province Harbin 150081, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Tong
authors.affiliation: Department of Microbiology, Harbin Medical University, Immunity and Infection, Pathogenic Biology Key Laboratory in Heilongjiang Province Harbin 150081, China.
authors.forename: Junming
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Microbiology, Harbin Medical University, Immunity and Infection, Pathogenic Biology Key Laboratory in Heilongjiang Province Harbin 150081, China.
authors.forename: Bowen
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Microbiology, Harbin Medical University, Immunity and Infection, Pathogenic Biology Key Laboratory in Heilongjiang Province Harbin 150081, China.
authors.forename: Lanlan
authors.initials: L
authors.lastname: Wei
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital, Harbin Medical University Harbin 150001, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001254:Astrocytoma / Q000378:metabolism / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000096:biosynthesis*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D011379:Prognosis; D016601:RNA-Binding Proteins; D060888:Real-Time Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; C483039:MTDH protein, human; D008565:Membrane Proteins; D016601:RNA-Binding Proteins
keywords: AEG-1; Astrocytoma; prognosis