title: The effect of common antineoplastic agents on induction of apoptosis in canine lymphoma and leukemia cell lines.
abstract: OBJECTIVE
  Lymphoma, the most common hematopoietic cancer in dogs is sensitive to chemotherapy which is the dominant treatment method. The aim of the present study was to evaluate the concentration-dependent cytotoxicity and ability to induce apoptosis of the anti-neoplastic agents cyclophosphamide (CYC), chlorambucil (CBL), cytosine arabinoside (ARA), dexamethasone (DEX), doxorubicin (DOX), etoposide (ETO), lomustine (LOM), prednisone (PRED) and vincristine (VINK) against GL-1, CL-1, CLBL-1 and Jurkat cell lines.
  
  
  METHODS
  To determine cell viability and level of apoptosis, three different tests were performed: Thiazolyl Blue Tetrazolium Bromide (MTT), annexin V/propidium iodide (An/PI) staining and flow cytometric DNA fragmentation.
  
  
  RESULTS
  All tested substances exhibited concentration-dependent inhibitory effects on the proliferation of the examined cell lines with a different level of apoptosis induction. VINK and DOX strongly decreased the viability of canine cell lines, whereas CYC induced the highest level of apoptosis.
  
  
  CONCLUSIONS
  Canine lymphoma (CL-1, CLBL-1) and leukemia (GL-1) cell lines are a useful tool for developing new and more effective treatment regimes for canine neoplasia.
authors.affiliation: Department of Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wroclaw, Poland aleksandra.pawlak@up.wroc.pl.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Pawlak
authors.affiliation: Laboratory of Tumor Molecular Immunobiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Rapak
authors.affiliation: Laboratory of Flow Cytometry, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Zbyryt
authors.affiliation: Department of Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wroclaw, Poland.
authors.forename: Bożena
authors.initials: B
authors.lastname: Obmińska-Mrukowicz
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004285:Dogs; D005434:Flow Cytometry; D020128:Inhibitory Concentration 50; D007938:Leukemia / Q000378:metabolism; D008223:Lymphoma / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents
keywords: Canine lymphoma; apoptosis; canine leukemia; cytotoxicity