title: Characterization of microsatellite markers for the vulnerable grassland forb Senecio macrocarpus (Asteraceae).
abstract: OBJECTIVE
  Development of microsatellite markers for the vulnerable forb Senecio macrocarpus was performed to begin an assessment of its population structure and breeding method to aid in the conservation of the species in Victoria, Australia. •
  
  
  RESULTS
  Fifteen microsatellite markers were developed for S. macrocarpus from 454 pyrosequencing. The markers were tested on 104 individuals from four populations. The markers produced between two and seven alleles per locus while the expected heterozygosity ranged from 0.20 to 0.67 and the observed heterozygosity ranged from 0.00 to 1.00. The observed heterozygosity is suggestive that the populations may be apomictic. •
  
  
  CONCLUSIONS
  The microsatellite markers developed for S. macrocarpus are intended to be used on future studies that aim to assess the population genetics and local breeding dynamics of the species with an emphasis on conservation.
authors.affiliation: Royal Botanic Gardens Melbourne, National Herbarium of Victoria, Private Bag 2000, Birdwood Avenue, South Yarra, Victoria 3141, Australia.
authors.forename: Collin W
authors.initials: CW
authors.lastname: Ahrens
authors.affiliation: Royal Botanic Gardens Melbourne, National Herbarium of Victoria, Private Bag 2000, Birdwood Avenue, South Yarra, Victoria 3141, Australia.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: James
keywords: 454 sequencing; Asteraceae; Senecio macrocarpus; apomixis; conservation; microsatellites; polyploidy