title: Developmental increase in hippocampal endocannabinoid mobilization: role of metabotropic glutamate receptor subtype 5 and phospholipase C.
abstract: Endocannabinoids (eCBs) released from postsynaptic neurons mediate retrograde suppression of neurotransmitter release at central synapses. eCBs are crucial for establishing proper synaptic connectivity in the developing nervous system. Mobilization of eCBs is driven either by a rise in intracellular Ca(2+) (depolarization-induced suppression of inhibition, DSI) or postsynaptic G protein-coupled receptors (GPCRs) that activate phospholipase C beta (PLCβ). To determine whether eCB mobilization changes between neonatal and juvenile ages, we used whole cell voltage-clamp recordings of CA1 neurons from rat hippocampal slices at postnatal days 1-18 (neonatal) and 19-43 (juvenile), because many neurophysiological parameters change dramatically between approximately postnatal days 18-20. We found that DSI was slightly greater in juveniles than in neonates, while eCB mobilization stimulated by GPCRs was unchanged. However, when DSI was elicited during GPCR activation, its increase was much greater in juveniles, suggesting that eCB mobilization caused by the synergy between the Ca(2+) and GPCR pathways is developmentally upregulated. Western blotting revealed significant increases in both metabotropic type glutamate receptor 5 (mGluR5) and PLCβ1 proteins in juveniles compared with neonates. Responses to pharmacological activation or inhibition of PLC implied that eCB upregulation is associated with a functional increase in PLC activity. We conclude that synergistic eCB mobilization in hippocampal CA1 neurons is greater in juveniles than in neonates, and that this may result from increases in the mGluR5-PLCβ1 eCB pathway. The data enhance our understanding of the developmental regulation of the eCB system and may provide insight into diseases caused by improper cortical wiring, or the impact of cannabis exposure during development.
authors.affiliation: Department of Physiology and Pharmacology, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan, Republic of China; slliang@mail.cgu.edu.tw.
authors.forename: Shu-Ling
authors.initials: SL
authors.lastname: Liang
authors.affiliation: Program in Neuroscience, University of Maryland School of Medicine, Baltimore, Maryland; Departments of Physiology and Psychiatry, University of Maryland School of Medicine, Baltimore, Maryland; and.
authors.forename: Bradley E
authors.initials: BE
authors.lastname: Alger
authors.affiliation: Program in Neuroscience, University of Maryland School of Medicine, Baltimore, Maryland; Departments of Physiology and Psychiatry, University of Maryland School of Medicine, Baltimore, Maryland; and Department of Pharmacology, University of Maryland School of Medicine, Baltimore, Maryland.
authors.forename: Margaret M
authors.initials: MM
authors.lastname: McCarthy
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D056547:CA1 Region, Hippocampal / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D063388:Endocannabinoids / Q000378:metabolism*; D005260:Female; D053444:Inhibitory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D008297:Male; D018408:Patch-Clamp Techniques; D054799:Phospholipase C beta / Q000037:antagonists & inhibitors / Q000378:metabolism*; D017966:Pyramidal Cells / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D017207:Rats, Sprague-Dawley; D043884:Receptor, Cannabinoid, CB1 / Q000378:metabolism; D064529:Receptor, Metabotropic Glutamate 5 / Q000819:agonists / Q000378:metabolism*; D018094:Receptors, Metabotropic Glutamate / Q000819:agonists / Q000378:metabolism; D046509:Tissue Culture Techniques
chemical_list: D063388:Endocannabinoids; C578937:Grm5 protein, rat; D043884:Receptor, Cannabinoid, CB1; D064529:Receptor, Metabotropic Glutamate 5; D018094:Receptors, Metabotropic Glutamate; C104077:metabotropic glutamate receptor type 1; D054799:Phospholipase C beta; C518035:Plcb1 protein, rat
keywords: CA1 region; DSI; G protein-coupled receptors; Western blot; mAChRs; whole cell voltage-clamp