title: Characterization of microsatellite loci in Castilleja sessiliflora and transferability to 24 Castilleja species (Orobanchaceae).
abstract: OBJECTIVE
  Microsatellite primers were developed in the hemiparasitic perennial forb Castilleja sessiliflora to investigate patterns of gene flow and genetic diversity within and among populations. •
  
  
  RESULTS
  Twelve polymorphic loci were identified in C. sessiliflora and tested on three populations (32 individuals each) sampled across the range of the species. The loci amplified di- and trinucleotide repeats with 3-14 alleles per locus. To assess cross-amplification, primer pairs were also tested on 24 additional Castilleja species that represent the morphological and geographic diversity of the genus. We provide reports of their effectiveness in all 25 taxa. •
  
  
  CONCLUSIONS
  These results indicate the utility of these primers in C. sessiliflora for future studies of genetic structure and gene flow, as well as their widespread applicability in other members of the diverse and complex genus Castilleja.
authors.affiliation: Plant Biology and Conservation, Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, Illinois 60022 USA.
authors.forename: Jeremie B
authors.initials: JB
authors.lastname: Fant
authors.affiliation: Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218 USA.
authors.forename: Hannah
authors.initials: H
authors.lastname: Weinberg-Wolf
authors.affiliation: College of Natural Resources and Stillinger Herbarium, University of Idaho, 875 Perimeter Drive MS 1133, Moscow, Idaho 83844-1133 USA.
authors.forename: David C
authors.initials: DC
authors.lastname: Tank
authors.affiliation: Plant Biology and Conservation, Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, Illinois 60022 USA.
authors.forename: Krissa A
authors.initials: KA
authors.lastname: Skogen
keywords: Castilleja; Castilleja sessiliflora; Orobanchaceae; cross-amplification; hemiparasite; microsatellites