title: Optical hyperspectral imaging in microscopy and spectroscopy - a review of data acquisition.
abstract: Rather than simply acting as a photographic camera capturing two-dimensional (x, y) intensity images or a spectrometer acquiring spectra (λ), a hyperspectral imager measures entire three-dimensional (x, y, λ) datacubes for multivariate analysis, providing structural, molecular, and functional information about biological cells or tissue with unprecedented detail. Such data also gives clinical insights for disease diagnosis and treatment. We summarize the principles underpinning this technology, highlight its practical implementation, and discuss its recent applications at microscopic to macroscopic scales. Datacube acquisition strategies in hyperspectral imaging x, y, spatial coordinates; λ, wavelength.
authors.affiliation: Department of Biomedical Engineering, Washington University in St. Louis, MO, 63139. gaol@wustl.edu.
authors.forename: Liang
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Ophthalmology, NYU School of Medicine, New York, NY, 10016. Roland.Smith@nyumc.org.
authors.forename: R Theodore
authors.initials: RT
authors.lastname: Smith
mesh_terms: D000818:Animals; D006801:Humans; D008853:Microscopy / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*; D013057:Spectrum Analysis / Q000379:methods*
keywords: biomedical imaging; hyperspectral imaging; multispectral imaging