title: Development of polymorphic microsatellite markers for Indian tobacco, Lobelia inflata (Campanulaceae)(1.).
abstract: OBJECTIVE
  Nuclear microsatellite markers were developed for Lobelia inflata (Campanulaceae), an obligately self-fertilizing plant species, for use in the study of temporal fluctuation in allele frequency and of the genetic structure within and among populations. •
  
  
  RESULTS
  We developed 28 primer pairs for L. inflata, all of which amplify CT dinucleotide repeats. We evaluated amplification of these loci in 53 L. inflata individuals at three sites in eastern North America and found that 24 loci showed microsatellite polymorphism. We also found that 16 loci amplified successfully in L. cardinalis, and 11 amplified successfully in L. siphilitica. •
  
  
  CONCLUSIONS
  These primers will be useful for assessing allelic diversity within and among populations of L. inflata, and show potential for use in congeneric species.
authors.affiliation: Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, K1S 5B6 Ontario, Canada.
authors.forename: P William
authors.initials: PW
authors.lastname: Hughes
authors.affiliation: Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, K1S 5B6 Ontario, Canada.
authors.forename: Allison F
authors.initials: AF
authors.lastname: Jaworski
authors.affiliation: Department of Biological Sciences, University of Alberta, Edmonton, T6G 2E9 Alberta, Canada.
authors.forename: Corey S
authors.initials: CS
authors.lastname: Davis
authors.affiliation: Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, K1S 5B6 Ontario, Canada.
authors.forename: Susan M
authors.initials: SM
authors.lastname: Aitken
authors.affiliation: Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, K1S 5B6 Ontario, Canada.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Simons
keywords: Campanulaceae; Lobelia; Lobelia cardinalis; Lobelia inflata; Lobelia siphilitica; microsatellite