title: An orthotopic mouse model of laryngeal squamous cell carcinoma.
abstract: OBJECTIVE
  This study aimed to create a reliable and reproducible orthotopic mouse model of laryngeal malignancy that recapitulates its biologic behavior, local invasiveness, and spread as seen in patients.
  
  
  METHODS
  Via direct laryngoscopy, human squamous cell carcinoma line FaDu (ATCC HTB-43) was implanted in the larynx (supraglottis and glottis) in nu/nu mice (n = 31). Animals were monitored with direct laryngoscopy and ultrasound for tumor growth and survival. Specimens of larynxes, neck lymphatics, livers, and lungs were removed for histopathologic evaluation to assess tumor extension, thyroid cartilage invasion, and nodal spread.
  
  
  RESULTS
  Thirty-one successful direct laryngoscopies were performed. Supraglottic and glottic tumor uptake was 100% and 25%, respectively. Median survival for the animals with supraglottic tumors was 35 days. Histopathologic evaluation revealed pre-epiglottic extension, paraglottic extension, thyroid cartilage invasion, and lymph node metastasis.
  
  
  CONCLUSIONS
  We describe the first orthotopic model for laryngeal cancer. Our model faithfully recapitulates the phenotype and malignant behavior that reproduces its natural biologic behavior as seen in laryngeal cancer patients. This model offers an opportunity to identify and specifically target therapy for larynx squamous cell carcinoma.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Rambam Health Care Campus, Haifa, Israel jacob_cohen@hotmail.com.
authors.forename: Jacob T
authors.initials: JT
authors.lastname: Cohen
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Rambam Health Care Campus, Haifa, Israel The Laboratory for Applied Cancer Research, Clinical Research Center at Rambam, Rambam Health Care Campus, The Technion, Israel Institute of Technology, Haifa, Israel.
authors.forename: Ziv
authors.initials: Z
authors.lastname: Gil
authors.affiliation: The Laboratory for Applied Cancer Research, Clinical Research Center at Rambam, Rambam Health Care Campus, The Technion, Israel Institute of Technology, Haifa, Israel.
authors.forename: Yoav
authors.initials: Y
authors.lastname: Binenbaum
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Rambam Health Care Campus, Haifa, Israel The Laboratory for Applied Cancer Research, Clinical Research Center at Rambam, Rambam Health Care Campus, The Technion, Israel Institute of Technology, Haifa, Israel.
authors.forename: Shorook
authors.initials: S
authors.lastname: Na'ara
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Rambam Health Care Campus, Haifa, Israel The Laboratory for Applied Cancer Research, Clinical Research Center at Rambam, Rambam Health Care Campus, The Technion, Israel Institute of Technology, Haifa, Israel.
authors.forename: Moran
authors.initials: M
authors.lastname: Amit
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D051379:Mice; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D013957:Thyroid Cartilage / Q000473:pathology
keywords: cancer; glottis; larynx; mouse; supraglottis