title: Normobaric oxygen for cerebral ischemic injury.
abstract: Oxygen inhalation has been shown to increase oxygen supply to tissues after cerebral ischemia/ reperfusion injury, protecting injured neural cells. However, hyperbaric oxygen may aggravate oxidative stress. By contrast, normobaric oxygen has the rapid and non-invasive characteristics and may have therapeutic effects on ischemic/hypoxic disease. Rats inhaled normobaric oxygen (95% O2) for 6 consecutive days, and then a rat model of focal cerebral ischemia was established. Nissl and 2,3,5-triphenyltetrazolium chloride (TTC) staining revealed that normobaric oxygen pretreatment improved neurological deficits and reduced infarct volume. Immunohistochemical staining and western blot assay revealed that the expression of hypoxia-inducible factor-1α, Notch-1, vascular endothelial growth factor and erythropoietin were increased. Behavioral studies also verified that neurological deficit scores increased. The hypoxia-inducible factor inhibitor 2-methoxyestradiol treatment at 1 hour before administration of normobaric oxygen could suppress the protective effect of normobaric oxygen. Given these observations, normobaric oxygen pretreatment may alleviate cerebral ischemic injury via the hypoxia-inducible factor signal pathway.
authors.affiliation: Department of Anatomy and Histoembryology, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Anatomy and Histoembryology, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Haimeng
authors.initials: H
authors.lastname: Cui
authors.affiliation: Department of Anatomy and Histoembryology, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Zihe
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Anatomy and Histoembryology, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Anatomy and Histoembryology, Peking University Health Science Center, Beijing 100191, China.
authors.forename: Changman
authors.initials: C
authors.lastname: Zhou
keywords: blood-brain barrier; brain injury; cerebral ischemia; focal cerebral ischemia; grants-supported paper; hypoxia-inducible factor-1α; neural regeneration; neuroregeneration; normobaric oxygen