title: Low expression of dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin in non-Hodgkin lymphoma and a significant correlation with β2-microglobulin.
abstract: Dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN), a member of the C-type lectin superfamily, has been reported to bind to various pathogens and several tumor cells and to participate in immunoregulation. It is still unclear whether there is a significant association between the level of DC-SIGN and non-Hodgkin lymphoma (NHL). To investigate the clinical diagnostic significance of DC-SIGN in NHL, we conducted a study with 52 NHL patients and 104 healthy individuals. Enzyme-linked immunosorbent assay and tissue microarray technology were utilized for the analysis. The serum sDC-SIGN levels in the NHL patients were significantly lower than those in the healthy controls (P=0.0019). A cutoff value of 1.499 µg/ml for sDC-SIGN predicted the presence of NHL with 78.85% sensitivity and 53.85% specificity [area under the curve (AUC)=0.6531, P=0.0019]. The serum sDC-SIGN levels in NHL patients were also significantly correlated with β2-microglobulin (P=0.0062). Moreover, tissue microarray analysis demonstrated that the expression of DC-SIGN in the lymph nodes or tissues of 96 NHL patients was significantly lower than that in 18 normal lymph nodes (P<0.0001). However, the expression of DC-SIGN in NHL displayed no significant correlation with the expression of CD20 or CD79a. In conclusion, DC-SIGN may be a promising biological molecule for clinical research on NHL, whereas the underlying roles need to be investigated in additional studies.
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, 116023, China.
authors.forename: Dongbing
authors.initials: D
authors.lastname: Ding
authors.forename: Wenbin
authors.initials: W
authors.lastname: Chen
authors.forename: Changfu
authors.initials: C
authors.lastname: Zhang
authors.forename: Zhe
authors.initials: Z
authors.lastname: Chen
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Jiang
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Yang
authors.forename: Xudong
authors.initials: X
authors.lastname: Jiang
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Zuo
authors.forename: Shuangyi
authors.initials: S
authors.lastname: Ren
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003713:Dendritic Cells; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism*; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012680:Sensitivity and Specificity; D046888:Tissue Array Analysis; D001613:beta 2-Microglobulin / Q000378:metabolism*; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015415:Biomarkers; D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D001613:beta 2-Microglobulin; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin