title: Structure of a DsbF homologue from Corynebacterium diphtheriae.
abstract: Disulfide-bond formation, mediated by the Dsb family of proteins, is important in the correct folding of secreted or extracellular proteins in bacteria. In Gram-negative bacteria, disulfide bonds are introduced into the folding proteins in the periplasm by DsbA. DsbE from Escherichia coli has been implicated in the reduction of disulfide bonds in the maturation of cytochrome c. The Gram-positive bacterium Mycobacterium tuberculosis encodes DsbE and its homologue DsbF, the structures of which have been determined. However, the two mycobacterial proteins are able to oxidatively fold a protein in vitro, unlike DsbE from E. coli. In this study, the crystal structure of a DsbE or DsbF homologue protein from Corynebacterium diphtheriae has been determined, which revealed a thioredoxin-like domain with a typical CXXC active site. Structural comparison with M. tuberculosis DsbF would help in understanding the function of the C. diphtheriae protein.
authors.affiliation: Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Si-Hyeon
authors.initials: SH
authors.lastname: Um
authors.affiliation: Department of Manufacturing Pharmacy, Pusan National University, Busan, Republic of Korea.
authors.forename: Jin-Sik
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul, Republic of Korea.
authors.forename: Kangseok
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Nam-Chul
authors.initials: NC
authors.lastname: Ha
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D020134:Catalytic Domain; D003353:Corynebacterium diphtheriae / Q000737:chemistry*; D003460:Crystallization; D017931:DNA Primers; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers
keywords: Dsb family; Gram-positive bacteria; disulfide isomerase