title: HS-SPME-GC-FID method for detection and quantification of Bacillus cereus ATCC 10702 mediated 2-acetyl-1-pyrroline.
abstract: A rapid micro-scale solid-phase micro-extraction (SPME) procedure coupled with gas-chromatography with flame ionized detector (GC-FID) was used to extract parts per billion levels of a principle basmati aroma compound "2-acetyl-1-pyrroline" (2-AP) from bacterial samples. In present investigation, optimization parameters of bacterial incubation period, sample weight, pre-incubation time, adsorption time, and temperature, precursors and their concentrations has been studied. In the optimized conditions, detection of 2-AP produced by Bacillus cereus ATCC10702 using only 0.5 g of sample volume was 85 μg/kg. Along with 2-AP, 15 other compounds produced by B. cereus were also reported out of which 14 were reported for the first time consisting mainly of (E)-2-hexenal, pentadecanal, 4-hydroxy-2-butanone, n-hexanal, 2-6-nonadienal, 3-methoxy-2(5H) furanone and 2-acetyl-1-pyridine and octanal. High recovery of 2-AP (87 %) from very less amount of B. cereus samples was observed. The method is reproducible fast and can be used for detection of 2-AP production by B. cereus.
authors.affiliation: Agronomy and Soil Science Division, CSIR-CIMAP, Lucknow, 226015, India.
authors.forename: Yogita
authors.initials: Y
authors.lastname: Deshmukh
authors.forename: Puja
authors.initials: P
authors.lastname: Khare
authors.forename: D D
authors.initials: DD
authors.lastname: Patra
authors.forename: Altafhusain B
authors.initials: AB
authors.lastname: Nadaf
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D001409:Bacillus cereus / Q000378:metabolism*; D019149:Bioreactors; D005410:Flame Ionization / Q000379:methods*; D011758:Pyrroles / Q000032:analysis* / Q000378:metabolism; D052617:Solid Phase Microextraction / Q000379:methods*
chemical_list: D000596:Amino Acids; D011758:Pyrroles; C426303:2-acetyl-1-pyrroline
keywords: 2-acetyl-1-pyrroline; Bacillus cereus; solid-phase micro-extraction