title: Influence of poly-N-acetylglucosamine in the extracellular matrix on N-chlorotaurine mediated killing of Staphylococcus epidermidis.
abstract: N-chlorotaurine (NCT) has recently been shown to have bactericidal activity against bacterial biofilm on metal discs (Coraca-Huber et al., 2014). In a biofilm, Staphylococcus epidermidis polymerizes poly-N-acetylglucosamine (PNAG) to form an extracellular matrix (ECM). Pseudomonas aeruginosa does not express this PNAG and has been shown to be highly susceptible to NCT. We compared the action of NCT on S. epidermidis 1457, a PNAG positive strain (SE1457) and S. epidermidis 1457- M10 an isogenic PNAG negative mutant (SE1457 M10). NCT-mediated killing was more effective and quicker on the PNAG negative strain SE1457 M10. Bacteria hidden in biofilms for prolonged periods of time were generally more susceptible than freshly formed biofilms. The differences in NCT-mediated killing might not be direct effects since NCT did not react with the monomeric N-acetylglucosamine, but might be explained by denser growth in the PNAG-containing biofilm produced by the wild type strain, which results in delayed penetration of NCT. The higher susceptibility of older biofilms to NCTmediated killing could be explained by more pronounced 3D architecture and subsequent larger surface area for interactions with NCT.
authors.affiliation: Experimental Orthopaedics, Innsbruck Medical University, Innsbruck, Austria.
authors.forename: Christoph G
authors.initials: CG
authors.lastname: Ammann
authors.forename: Manfred
authors.initials: M
authors.lastname: Fille
authors.forename: Johann
authors.initials: J
authors.lastname: Hausdorfer
authors.forename: Michael
authors.initials: M
authors.lastname: Nogler
authors.forename: Markus
authors.initials: M
authors.lastname: Nagl
authors.forename: Débora C
authors.initials: DC
authors.lastname: Coraça-Huber
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D005109:Extracellular Matrix / Q000378:metabolism*; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D013654:Taurine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D013654:Taurine; C043410:N-chlorotaurine; D000117:Acetylglucosamine