title: Development of microsatellite markers for Vitex rotundifolia (Verbenaceae), an endangered coastal plant in Lake Biwa, Japan.
abstract: OBJECTIVE
  Microsatellite loci were developed for Vitex rotundifolia, an endangered species isolated to Lake Biwa, to investigate its genetic diversity and population structure. •
  
  
  RESULTS
  Ten primer sets were identified in Japanese populations of V. rotundifolia. The number of alleles per locus ranged from one to six and gene diversity per locus ranged from 0.040 to 0.697 between two populations. In addition, all loci could be successfully amplified in V. trifolia. •
  
  
  CONCLUSIONS
  These markers will be useful for studies of genetic diversity and population structure of endangered species isolated to Lake Biwa of V. rotundifolia, to aid in the development of conservation strategies.
authors.affiliation: Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Ohtsuki
authors.affiliation: Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8224, Japan.
authors.forename: Tasuku
authors.initials: T
authors.lastname: Shoda
authors.affiliation: Lake Biwa Environmental Research Institute, 5-34 Yanagasaki, Otsu-city, Shiga 520-0022, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kaneko
authors.affiliation: Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Setoguchi
keywords: Vitex rotundifolia; Vitex trifolia; conservation genetics; gene diversity; microsatellite; population structure