title: Microarray analysis of gene expression in lung cancer cell lines treated by fractionated irradiation.
abstract: BACKGROUND
  To identify differentially expressed genes between parent and radioresistant lung cancer cell lines established by fractionated irradiation.
  
  
  METHODS
  Lung cancer cell lines (A549, NCI-H1650) were irradiated with several fractionation schemes. Clonogenic assays were used to identify radioresistant cell lines. We compared the gene expression profiles on a cDNA microarray.
  
  
  RESULTS
  Four established cell (A549-2G, A549-5G, H1650-2G and H1650-5G) were shown to be radioresistant (p≤0.05). Seventy-two genes were commonly altered in A549-G and 655 genes in H1650-G, compared to their parental cells. Genes in the wingless-type MMTV integration site family (WNT) signaling pathway were the ones most frequently altered in both A549-G and H1650-G cells. Those involved in inflammation; integrin, platelet-derived growth factor (PDGF), interleukin, transforming growth factor-beta (TGFB), epidermal growth factor receptor (EGFR) signaling, were commonly altered in radioresistant H1650 sublines.
  
  
  CONCLUSIONS
  The major gene expression changes during irradiation are related to WNT signaling pathway.
authors.affiliation: Department of Radiation Oncology, Chonnam National University Medical School, Gwangju, Republic of Korea.
authors.forename: Sung-Ja
authors.initials: SJ
authors.lastname: Ahn
authors.affiliation: Department of Pathology, Chonnam National University Medical School, Gwangju, Republic of Korea cchoi@chonnam.ac.kr.
authors.forename: Chan
authors.initials: C
authors.lastname: Choi
authors.affiliation: Department of Pathology, Chonnam National University Medical School, Gwangju, Republic of Korea.
authors.forename: Yoo-Duk
authors.initials: YD
authors.lastname: Choi
authors.affiliation: Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Republic of Korea.
authors.forename: Young-Chul
authors.initials: YC
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Republic of Korea.
authors.forename: Kyu-Sik
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Republic of Korea.
authors.forename: In-Jae
authors.initials: IJ
authors.lastname: Oh
authors.affiliation: Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Republic of Korea.
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Ban
authors.affiliation: Department of Radiation Oncology, Chonnam National University Medical School, Gwangju, Republic of Korea.
authors.forename: Mee-Sun
authors.initials: MS
authors.lastname: Yoon
authors.affiliation: Department of Radiation Oncology, Chonnam National University Medical School, Gwangju, Republic of Korea.
authors.forename: Taek-Keun
authors.initials: TK
authors.lastname: Nam
authors.affiliation: Department of Radiation Oncology, Chonnam National University Medical School, Gwangju, Republic of Korea.
authors.forename: Jae-Uk
authors.initials: JU
authors.lastname: Jeong
authors.affiliation: Department of Radiation Oncology, Chonnam National University Medical School, Gwangju, Republic of Korea.
authors.forename: Joo-Young
authors.initials: JY
authors.lastname: Song
authors.affiliation: Department of Radiation Oncology, Chonnam National University Medical School, Gwangju, Republic of Korea.
authors.forename: Woong-Ki
authors.initials: WK
authors.lastname: Chung
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D016000:Cluster Analysis; D019583:Dose Fractionation, Radiation; D004307:Dose-Response Relationship, Radiation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000532:radiotherapy; D011836:Radiation Tolerance / Q000235:genetics; D015203:Reproducibility of Results
keywords: Non-small cell lung cancer; cDNA miroarrays; fractionated irradiation; radioresistance