title: Sciatic nerve regeneration using a nerve growth factor-containing fibrin glue membrane.
abstract: Our previous findings confirmed that the nerve growth factor-containing fibrin glue membrane provides a good microenvironment for peripheral nerve regeneration; however, the precise mechanism remains unclear. p75 neurotrophin receptor (p75(NTR)) plays an important role in the regulation of peripheral nerve regeneration. We hypothesized that a nerve growth factor-containing fibrin glue membrane can promote neural regeneration by up-regulating p75(NTR) expression. In this study, we used a silicon nerve conduit to bridge a 15 mm-long sciatic nerve defect and injected a mixture of nerve growth factor and fibrin glue at the anastomotic site of the nerve conduit and the sciatic nerve. Through RT-PCR and western blot analysis, nerve growth factor-containing fibrin glue membrane significantly increased p75(NTR) mRNA and protein expression in the Schwann cells at the anastomotic site, in particular at 8 weeks after injection of the nerve growth factor/fibrin glue mixture. These results indicate that nerve growth factor-containing fibrin glue membrane can promote peripheral nerve regeneration by up-regulating p75(NTR) expression in Schwann cells.
authors.affiliation: Department of Orthopedics, Second Affiliated Hospital of Shandong University, Jinan 250033, Shandong Province, China.
authors.forename: Shengzhong
authors.initials: S
authors.lastname: Ma
authors.affiliation: Department of Orthopedics, Second Affiliated Hospital of Shandong University, Jinan 250033, Shandong Province, China.
authors.forename: Changliang
authors.initials: C
authors.lastname: Peng
authors.affiliation: Department of Orthopedics, Second Affiliated Hospital of Shandong University, Jinan 250033, Shandong Province, China.
authors.forename: Shiqing
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Orthopedics, Second Affiliated Hospital of Shandong University, Jinan 250033, Shandong Province, China.
authors.forename: Dongjin
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Orthopedics, Second Affiliated Hospital of Shandong University, Jinan 250033, Shandong Province, China.
authors.forename: Chunzheng
authors.initials: C
authors.lastname: Gao
keywords: Schwann cells; fibrin glue; grants-supported paper; microenvironment; nerve growth factor; nerve growth factor-containing fibrin glue membrane; neural regeneration; neuroregeneration; p75 neurotrophin receptor; peripheral nerve injury; peripheral nerve regeneration; sciatic nerve