title: Antioxidant and anti-inflammatory effects of selected natural compounds contained in a dietary supplement on two human immortalized keratinocyte lines.
abstract: Several advantages may derive from the use of dietary supplements containing multiple natural antioxidants and/or anti-inflammatory agents. At present, however, there is scarce information on the properties and potential of combined supplements. To fill the gap, the antioxidant and anti-inflammatory activities exerted by a combination of seven natural components (coenzyme Q10, krill oil, lipoic acid, resveratrol, grape seed oil, α-tocopherol, and selenium) contained in a dietary supplement used for the prevention of skin disorders were investigated in vitro. Each component was administered, alone or in combination, to human keratinocytes, and the inhibition of Reactive Oxygen Species production and lipid peroxidation as well as the ability to reduce inflammatory cytokine secretion and to modulate Nuclear Factor-κB pathway was evaluated. The combination exhibited high antioxidant activity and in specific conditions the combination's efficiency was higher than that of the most powerful components administered individually. Moreover, the combination showed remarkable anti-inflammatory properties. It reduced more efficiently than each component the secretion of Monocyte Chemoattractant Protein-1, a crucial cytokine for the development of chronic inflammation in skin, and inhibited Nuclear Factor-κB molecular pathway. Overall, our findings suggest that the combined formulation may have the potential to powerfully inhibit oxidative stress and inflammation at skin level.
authors.affiliation: Institute of General Pathology, Catholic University, 00168 Rome, Italy.
authors.forename: Elena
authors.identifier: 0000-0002-4040-876X
authors.initials: E
authors.lastname: Fasano
authors.affiliation: Institute of General Pathology, Catholic University, 00168 Rome, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Serini
authors.affiliation: Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Arcavacata di Rende, 87030 Cosenza, Italy.
authors.forename: Nadia
authors.initials: N
authors.lastname: Mondella
authors.affiliation: Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Arcavacata di Rende, 87030 Cosenza, Italy.
authors.forename: Sonia
authors.identifier: 0000-0001-9939-4457
authors.initials: S
authors.lastname: Trombino
authors.affiliation: Institute of Clinical Dermatology, Catholic University, 00168 Rome, Italy ; Research Center for Biotechnology Applied to Cosmetology, Catholic University, 00168 Rome, Italy.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Celleno
authors.affiliation: Institute of Pathology, Catholic University, 00168 Rome, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Lanza
authors.affiliation: Institute of General Pathology, Catholic University, 00168 Rome, Italy.
authors.forename: Achille
authors.identifier: 0000-0002-6784-166X
authors.initials: A
authors.lastname: Cittadini
authors.affiliation: Institute of General Pathology, Catholic University, 00168 Rome, Italy ; Research Center for Biotechnology Applied to Cosmetology, Catholic University, 00168 Rome, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Calviello
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001688:Biological Products / Q000494:pharmacology*; D002461:Cell Line, Transformed; D018932:Chemokine CCL2 / Q000378:metabolism; D019587:Dietary Supplements; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D020672:I-kappa B Proteins / Q000378:metabolism; D007249:Inflammation / Q000473:pathology; D015850:Interleukin-6 / Q000096:biosynthesis; D053535:Keratin-13 / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D000072000:NF-KappaB Inhibitor alpha; D018384:Oxidative Stress / Q000187:drug effects; D010766:Phosphorylation / Q000187:drug effects; D010938:Plant Oils / Q000494:pharmacology; D024502:alpha-Tocopherol / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001688:Biological Products; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D020672:I-kappa B Proteins; D015850:Interleukin-6; D053535:Keratin-13; C000606204:NFKBIA protein, human; D010938:Plant Oils; D000072000:NF-KappaB Inhibitor alpha; D006861:Hydrogen Peroxide; D024502:alpha-Tocopherol