title: Multiplication and growth of hybrid poplar (Populus alba × P. tremula) shoots on a hormone-free medium.
abstract: The present study explored an alternative approach for poplar micropropagation, based on the restriction of gas exchange between inside and outside environments of culture vessel, rather than on the application of exogenous hormones. Apical and nodal stem segments (explants) excised from in vitro-developed shoots of hybrid white poplar (Populus alba L. × P. tremula L.) were incubated in either sealed (with Parafilm) or unsealed capped glass culture tubes (150 × 20 mm) on a hormone-free Woody Plant Medium. Shoot proliferation on apical explants was observed in sealed culture tubes but not in the unsealed ones; the difference between these two samples in respect of shoot number increased in the course of time and became threefold after three months of culture, with 3.2 ± 0.4 (mean ± SE) shoots per explant in the sealed tubes versus 1.1 ± 0.1 in the unsealed ones (for comparison, the mean shoot numbers on nodal explants were 2.4 ± 0.3 and 3.4 ± 0.4 in the unsealed and sealed culture tubes, respectively). Moreover, the shoots taken from the sealed culture tubes could be distinguished by superior shoot length, if compared to the shoots from the unsealed tubes, during the subsequent culture stage under uniform conditions.
authors.affiliation: Lithuanian Research Centre for Agriculture and Forestry Institute of Forestry Liepų g. 1, Girionys LT-53101 Kaunas distr. Lithuania.
authors.forename: J
authors.initials: J
authors.lastname: Ziauka
authors.affiliation: Lithuanian Research Centre for Agriculture and Forestry Institute of Forestry Liepų g. 1, Girionys LT-53101 Kaunas distr. Lithuania.
authors.forename: Sigutė
authors.initials: S
authors.lastname: Kuusienė
mesh_terms: D049109:Cell Proliferation; D019069:Cell Respiration; D003433:Crosses, Genetic; D003470:Culture Media / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D032107:Populus / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D012038:Regeneration; D013997:Time Factors; D046509:Tissue Culture Techniques
chemical_list: D003470:Culture Media
keywords: Culture tube; explant; micropropagation; restricted ventilation; white poplar