title: Pretreatment with scutellaria baicalensis stem-leaf total flavonoid prevents cerebral ischemia-reperfusion injury.
abstract: Pretreatment with scutellaria baicalensis stem-leaf total flavonoid has protective effects against ischemia and attenuates myocardial ischemia-reperfusion injury. In this study, rats were given scutellaria baicalensis stem-leaf total flavonoid intragastrically at 50, 100, and 200 mg/kg per day for 7 days before focal cerebral ischemia-reperfusion injury models were established using the suture method. We then determined the protective effects of scutellaria baicalensis stem-leaf total flavonoid pretreatment on focal cerebral ischemia-reperfusion injury. Results showed that neurological deficit scores increased, infarct volumes enlarged, apoptosis increased and Bcl-2 and Bax protein expression were upregulated at 24 hours after reperfusion. Pretreatment with scutellaria baicalensis stem-leaf total flavonoid at any dose lowered the neurological deficit scores, reduced the infarct volume, prevented apoptosis in hippocampal cells, attenuated neuronal and blood-brain barrier damage and upregulated Bcl-2 protein expression but inhibited Bax protein expression. Doses of 100 and 200 mg/kg were the most efficacious. Our findings indicate that pretreatment with scutellaria baicalensis stem-leaf total flavonoid at 100 and 200 mg/kg can improve the neurological functions and have preventive and protective roles after focal cerebral ischemia-reperfusion injury.
authors.affiliation: Laboratory of Spinal Cord Injury and Rehabilitation, Chengde Medical College, Chengde 067000, Hebei Province, China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Laboratory of Spinal Cord Injury and Rehabilitation, Chengde Medical College, Chengde 067000, Hebei Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Kong
authors.affiliation: Laboratory of Spinal Cord Injury and Rehabilitation, Chengde Medical College, Chengde 067000, Hebei Province, China.
authors.forename: Shufeng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Laboratory of Spinal Cord Injury and Rehabilitation, Chengde Medical College, Chengde 067000, Hebei Province, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Chen
authors.affiliation: Laboratory of Spinal Cord Injury and Rehabilitation, Chengde Medical College, Chengde 067000, Hebei Province, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Laboratory of Spinal Cord Injury and Rehabilitation, Chengde Medical College, Chengde 067000, Hebei Province, China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Kong
keywords: apoptosis; brain injury; grants-supported paper; hippocampus; ischemia-reperfusion injury; neural regeneration; neuroregeneration; pretreatment; scutellaria baicalensis stem-leaf total flavonoid