title: Development of microsatellite markers in Cratylia mollis and their transferability to C. argentea (Fabaceae).
abstract: OBJECTIVE
  This work aimed to develop microsatellite markers for Cratylia mollis as tools to assess its genetic diversity and structure and to evaluate their potential cross-amplification in related species. •
  
  
  RESULTS
  Microsatellite markers were developed using a microsatellite-enriched library and an intersimple sequence repeat library. From a set of 19 markers, 12 microsatellite loci were polymorphic and presented considerable variation in allele number (2-11), expected heterozygosity (0.226-0.883), and polymorphism information content per locus (0.212-0.870). Cross-amplification in C. argentea was successful in 16 loci, 12 of which were polymorphic (2-10 alleles). •
  
  
  CONCLUSIONS
  The polymorphism of this set of microsatellite markers for C. mollis, as well as their successful cross-amplification in C. intermedia and C. bahiensis and their transferability to C. argentea, supports their use in future comparative studies to understand the mechanism involved in population divergence and speciation in the genus.
authors.affiliation: Departamento Ciências Biológicas, Universidade Estadual de Feira de Santana (UEFS), Av. Transnordestina s.n., 44036-900 Feira de Santana, Bahia, Brazil.
authors.forename: M Cristina
authors.initials: MC
authors.lastname: López-Roberts
authors.affiliation: Departamento Ciências Biológicas, Universidade Estadual de Feira de Santana (UEFS), Av. Transnordestina s.n., 44036-900 Feira de Santana, Bahia, Brazil.
authors.forename: Luciano Paganucci
authors.initials: LP
authors.lastname: de Queiroz
authors.affiliation: Departamento Ciências Biológicas, Universidade Estadual de Feira de Santana (UEFS), Av. Transnordestina s.n., 44036-900 Feira de Santana, Bahia, Brazil.
authors.forename: Cássio
authors.initials: C
authors.lastname: van den Berg
keywords: Cratylia; Fabaceae; cross-amplification; microsatellite; population divergence; transferability