title: Enzyme encapsulation in magnetic chitosan-Fe3O4 microparticles.
abstract: Two simple procedures for the preparation of magnetic chitosan enzyme microparticles have been investigated and used for the immobilisation of endophytic fungus Cercospora kikuchii lipase as model enzyme. In the first case, lipase was entrapped in Fe3O4-chitosan microparticles by cross-linking method, while in the second case magnetic immobilised derivatives were produced using spray drying. Immobilised enzymes showed high enzyme activity retention and stability during storage without significant loss of activity. Glutaraldehyde Fe3O4-chitosan powders presented a higher lipase activity retention and storage stability than the others preparations. However, the immobilised derivatives produced by cross-linking showed higher enzyme activity after reuse cycles. The results proved that the magnetic Fe3O4-chitosan microparticles are an effective support for the enzyme immobilisation since the immobilised lipase showed best properties than the free form.
authors.affiliation: School of Pharmaceutical Sciences of Ribeirão Preto, Laboratory of Pharmaceutical Processes (LAPROFAR), University of São Paulo , Ribeirão Preto , Brazil.
authors.forename: Tales Alexandre
authors.initials: TA
authors.lastname: Costa-Silva
authors.forename: Polyana Samorano
authors.initials: PS
authors.lastname: Marques
authors.forename: Cláudia Regina Fernandes
authors.initials: CR
authors.lastname: Souza
authors.forename: Suraia
authors.initials: S
authors.lastname: Said
authors.forename: Wanderley Pereira
authors.initials: WP
authors.lastname: Oliveira
mesh_terms: D001203:Ascomycota / Q000201:enzymology*; D048271:Chitosan / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D048271:Chitosan; D008049:Lipase; D052203:Ferrosoferric Oxide
keywords: Bioencapsulation; Cercospora kikuchii; chitosan; enzyme immobilisation; lipase; spray dryer