title: Crystallization and preliminary X-ray analysis of the C-terminal fragment of Ski7 from Saccharomyces cerevisiae.
abstract: Ski7 (superkiller protein 7) plays a critical role in the mRNA surveillance pathway. The C-terminal fragment of Ski7 (residues 520-747) from Saccharomyces cerevisiae was heterologously expressed in Escherichia coli and purified to homogeneity. It was successfully crystallized and preliminary X-ray data were collected to 2.0 Å resolution using synchrotron radiation. The crystal belonged to a trigonal space group, either P3121 or P3221, with unit-cell parameters a = b = 73.5, c = 83.6 Å. The asymmetric unit contains one molecule of the C-terminal fragment of Ski7 with a corresponding crystal volume per protein mass (VM) of 2.61 Å(3) Da(-1) and a solvent content of 52.8% by volume. The merging R factor is 6.6%. Structure determination by MAD phasing is under way.
authors.affiliation: Department of Life Sciences, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 136-701, Republic of Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Life Sciences, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 136-701, Republic of Korea.
authors.forename: Si Hoon
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Life Sciences, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 136-701, Republic of Korea.
authors.forename: Byung-Cheon
authors.initials: BC
authors.lastname: Jeong
authors.affiliation: Department of Life Sciences, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 136-701, Republic of Korea.
authors.forename: Hyun Kyu
authors.initials: HK
authors.lastname: Song
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry*; D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D010446:Peptide Fragments / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D017931:DNA Primers; D010446:Peptide Fragments; C118668:SKI7 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins
keywords: RNA degradation; Saccharomyces cerevisiae; Ski7; mRNA surveillance; nonstop decay