title: Synthesis of branched DNA using oxidatively cleavable tritylsulfenyl as a hydroxy protecting group.
abstract: The application of oxidatively cleavable tritylsulfenyl (TrS) group to the synthesis of branched DNA is described. The TrS protecting group can be removed by treatment with 1 M aqueous iodine, while it is stable toward an oxaziridine-type oxidant. At the same time, the sulfur-oxygen linkage showed sufficient stability under the acidic and basic conditions used in oligonucleotide synthesis. These properties of the TrS group enabled the synthesis of branched DNA using a branched phosphoramidite in which the two hydroxy groups are protected by a 4,4'-dimethoxytrityl (DMTr) group or a TrS group. In this unit, we describe an example of the synthesis of a three-way branched DNA using a branched phosphoramidite.
authors.affiliation: Department of Life Science, Tokyo Institute of Technology, Tokyo, Japan.
authors.forename: Kohji
authors.initials: K
authors.lastname: Seio
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanamori
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sekine
mesh_terms: D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry*
chemical_list: D004247:DNA
keywords: branched DNA; hydroxy protecting group; tritylsulfenyl group