title: Comparison of waterborne and in ovo nanoinjection exposures to assess effects of PFOS on zebrafish embryos.
abstract: Since perfluorooctane sulfonate (PFOS) had been detected in eggs of seabirds and fish, toxicity of waterborne PFOS to embryonic development of zebrafish (Danio rerio) was investigated. However, because assessment of effects by use of dietary exposure of adults is time-consuming and expensive, a study was conducted to compare effects on embryos via nanoinjection and waterborne exposure. Nanoinjection, in which small amounts of chemicals are injected into developing eggs, was used to incorporate PFOS into the yolk sac of embryos of zebrafish. Effects of PFOS during the period of development of the embryo were assessed within 96 h post-fertilization (hpf). PFOS significantly retarded development of embryos of zebrafish and resulted in abnormalities as well as lethality of developing embryos. Both methods of exposure, waterborne and nanoinjection, resulted in similar dose-dependent effects. Some sublethal effects, such as edema at 48 hpf, delayed hatching, and curvature of the spine was observed after 72 hpf. In ovo, nanoinjection was deemed to be an accurate method of exposure for controlling the actual internal dose for study of adverse effects, which closely mimicked responses to waterborne exposure of zebrafish embryo to PFOS.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China.
authors.forename: Yabing
authors.initials: Y
authors.lastname: Li
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Han
authors.forename: Xinmei
authors.initials: X
authors.lastname: Zheng
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Ma
authors.forename: Hongling
authors.initials: H
authors.lastname: Liu
authors.forename: John P
authors.initials: JP
authors.lastname: Giesy
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Xie
authors.forename: Hongxia
authors.initials: H
authors.lastname: Yu
mesh_terms: D017738:Alkanesulfonic Acids / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D005466:Fluorocarbons / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D013723:Teratogens / Q000633:toxicity*; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity*; D015027:Zebrafish / Q000196:embryology
chemical_list: D017738:Alkanesulfonic Acids; D005466:Fluorocarbons; D013723:Teratogens; D014874:Water Pollutants, Chemical; C076994:perfluorooctane sulfonic acid