title: Development and characterization of EST-SSR markers for the Solidago virgaurea complex (Asteraceae) in the Japanese archipelago.
abstract: OBJECTIVE
  We developed simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) for the Solidago virgaurea complex, an ecologically and morphologically diverse species complex in the Japanese archipelago, to elucidate population genetic structure and examine taxonomic boundaries. •
  
  
  RESULTS
  Utilizing the RNA sequencing data obtained by next-generation sequencing techniques, 15 polymorphic EST-SSR markers with three to 14 alleles were developed, most of which were transferable to different Solidago species native to Eurasia and North America. •
  
  
  CONCLUSIONS
  The EST-SSR markers developed in this study may be useful for elucidating the population structure and taxonomic delimitation of the species complex, as well as for investigating the population genetics and reproductive ecology of Solidago species.
authors.affiliation: Laboratory of Plant Evolution and Biodiversity, Department of General Systems Studies, Graduate School of Arts and Sciences, University of Tokyo, Meguro, Tokyo 153-0041, Japan ; Research Fellow of the Japan Society for the Promotion of Science, Chiyoda, Tokyo 102-8472, Japan.
authors.forename: Shota
authors.initials: S
authors.lastname: Sakaguchi
authors.affiliation: Laboratory of Plant Evolution and Biodiversity, Department of General Systems Studies, Graduate School of Arts and Sciences, University of Tokyo, Meguro, Tokyo 153-0041, Japan.
authors.forename: Motomi
authors.initials: M
authors.lastname: Ito
keywords: Asteraceae; Solidago; expressed sequence tag; genetic structure; microsatellite