title: Increased cytogenetic abnormalities in exfoliated oral mucosal cells of South Indian foundry workers.
abstract: Biomonitoring offers a valuable tool to estimate the genetic risk as of exposure to genotoxic agents. Here, we intend to assess the potential cytogenetic damage related with occupational exposure to polycyclic aromatic hydrocarbons by evaluating the genetic damages in exfoliated buccal epithelial cells of foundry workers via counting micronucleus (MNs) and other nuclear abnormalities (NAs). This was a cross-sectional study and all study subjects were male . Exfoliated buccal mucosal cells were obtained from 100 subjects involved in either foundry molding or melting processes, and 100 controls matched for sex, age, and smoking from the area of Coimbatore city, Southern India. For each individual, 2000 exfoliated buccal cells were analyzed. Significantly, there was a higher frequency of MN in the exposed workers than in the controls (P < 0.05). Smoking was associated with the increased frequencies of micronuclei and NAs in the buccal epithelium of both the control and the exposed groups. Smoking represented significant factors in terms of increasing the production of MN when the control and the exposed groups were compared (P < 0.05). The results specify that buccal cells of foundry workers display increased levels of genotoxicity and these biomarker responses may be related to the increased cancer risk. These results conclude that the studied individuals are at a risk group and they require periodical biological monitoring and proper care which is essential for them.
authors.affiliation: Molecular Diagnosis and Drug Discovery Laboratory, Department of Biotechnology, School of Life Sciences, Karpagam University, Coimbatore, Tamilnadu, India, rssaranya.ya@gmail.com.
authors.forename: Saranya Ramalingam
authors.initials: SR
authors.lastname: Singaravelu
authors.forename: Sudha
authors.initials: S
authors.lastname: Sellappa
mesh_terms: D000328:Adult; D000395:Air Pollutants, Occupational / Q000633:toxicity*; D016022:Case-Control Studies; D002869:Chromosome Aberrations / Q000139:chemically induced*; D003430:Cross-Sectional Studies; D004249:DNA Damage; D004784:Environmental Monitoring; D004847:Epithelial Cells / Q000187:drug effects; D006801:Humans; D007194:India; D019570:Inhalation Exposure; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000187:drug effects / Q000473:pathology*; D016273:Occupational Exposure; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D012907:Smoking / Q000009:adverse effects; D055815:Young Adult
chemical_list: D000395:Air Pollutants, Occupational; D011084:Polycyclic Aromatic Hydrocarbons