title: Current development in non-enzymatic lipid peroxidation products, isoprostanoids and isofuranoids, in novel biological samples.
abstract: Isoprostanoids and isofuranoids are lipid mediators that can be formed from omega-3 and omega-6 polyunsaturated fatty acids (PUFAs). F2-isoprostanes formed from arachidonic acid, especially 15-F2t-isoprostane, are commonly measured in biological tissues for decades as the biomarker for oxidative stress and diseases. Recently, other forms of isoprostanoids derived from adrenic, eicosapentaenoic, and docosahexaenoic acids namely F2-dihomo-isoprostanes, F3-isoprostanes, and F4-neuroprostanes respectively, and isofuranoids including isofurans, dihomo-isofurans, and neurofurans are reported as oxidative damage markers for different metabolisms. The most widely used samples in measuring lipid peroxidation products include but not limited to the blood and urine; other biological fluids, specialized tissues, and cells can also be determined. In this review, measurement of isoprostanoids and isofuranoids in novel biological samples by gas chromatography (GC)-mass spectrometry (MS), GC-MS/MS, liquid chromatography (LC)-MS, and LC-MS/MS will be discussed.
authors.affiliation: School of Biological Sciences, The University of Hong Kong , Hong Kong.
authors.forename: K S
authors.initials: KS
authors.lastname: Leung
authors.forename: J M
authors.initials: JM
authors.lastname: Galano
authors.forename: T
authors.initials: T
authors.lastname: Durand
authors.forename: J C-Y
authors.initials: JC
authors.lastname: Lee
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D002853:Chromatography, Liquid; D005663:Furans / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D028421:Isoprostanes / Q000032:analysis*; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D005663:Furans; D028421:Isoprostanes
keywords: adrenic acid; dihomo-isofurans; dihomo-isoprostanes; isoprostanes; neuroprostanes