title: Genome sequence of Ensifer medicae strain WSM1115; an acid-tolerant Medicago-nodulating microsymbiont from Samothraki, Greece.
abstract: Ensifer medicae strain WSM1115 forms effective nitrogen fixing symbioses with a range of annual Medicago species and is used in commercial inoculants in Australia. WSM1115 is an aerobic, motile, Gram-negative, non-spore-forming rod. It was isolated from a nodule recovered from the root of burr medic (Medicago polymorpha) collected on the Greek Island of Samothraki. WSM1115 has a broad host range for nodulation and N2 fixation capacity within the genus Medicago, although this does not extend to all medic species. WSM1115 is considered saprophytically competent in moderately acid soils (pH(CaCl2) 5.0), but it has failed to persist at field sites where soil salinity exceeded 10 ECe (dS/m). Here we describe the features of E. medicae strain WSM1115, together with genome sequence information and its annotation. The 6,861,065 bp high-quality-draft genome is arranged into 7 scaffolds of 28 contigs, contains 6,789 protein-coding genes and 83 RNA-only encoding genes, and is one of 100 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
authors.forename: Wayne
authors.initials: W
authors.lastname: Reeve
authors.affiliation: South Australian Research and Development Institute, Urrbrae, South Australia, Australia.
authors.forename: Ross
authors.initials: R
authors.lastname: Ballard
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
authors.forename: John
authors.initials: J
authors.lastname: Howieson
authors.affiliation: South Australian Research and Development Institute, Urrbrae, South Australia, Australia.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Drew
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
authors.forename: Rui
authors.initials: R
authors.lastname: Tian
authors.affiliation: School of Life and Environmental Sciences, Deakin University, Victoria, Australia.
authors.forename: Lambert
authors.initials: L
authors.lastname: Bräu
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors.forename: Christine
authors.initials: C
authors.lastname: Munk
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors.forename: Karen
authors.initials: K
authors.lastname: Davenport
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors.forename: Patrick
authors.initials: P
authors.lastname: Chain
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors.forename: Lynne
authors.initials: L
authors.lastname: Goodwin
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Ioanna
authors.initials: I
authors.lastname: Pagani
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Marcel
authors.initials: M
authors.lastname: Huntemann
authors.affiliation: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Mavrommatis
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Amrita
authors.initials: A
authors.lastname: Pati
authors.affiliation: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Victor
authors.initials: V
authors.lastname: Markowitz
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Ivanova
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Tanja
authors.initials: T
authors.lastname: Woyke
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Nikos
authors.initials: N
authors.lastname: Kyrpides
keywords: Alphaproteobacteria; nitrogen fixation; rhizobia; root-nodule bacteria