title: Antibacterial and anti-inflammatory activity of a temporin B peptide analogue on an in vitro model of cystic fibrosis.
abstract: Natural peptides with antimicrobial properties are deeply investigated as tools to fight bacteria resistant to common antibiotics. Small peptides, as those belonging to the temporin family, are very attractive because their activity can easily be tuned after small modification to their primary sequence. Structure-activity studies previously reported by us allowed the identification of one peptide, analogue of temporin B, TB_KKG6A, showing, unlike temporin B, antimicrobial activity against both Gram-positive and Gram-negative bacteria. In this paper, we investigated the antimicrobial and anti-inflammatory activity of the peptide TB_KKG6A against Pseudomonas aeruginosa. Interestingly, we found that the peptide exhibits antimicrobial activity at low concentrations, being able to downregulate the pro-inflammatory chemokines and cytokines interleukin (IL)-8, IL-1β, IL-6 and tumor necrosis factor-α produced downstream infected human bronchial epithelial cells. Experiments were carried out also with temporin B, which was found to show pro-inflammatory activity. Details on the interaction between TB_KKG6A and the P. aeruginosa LPS were obtained by circular dichroism and fluorescence studies.
authors.affiliation: Dipartimento di Patologia e Diagnostica, Università di Verona, 37134, Verona, Italy.
authors.forename: Valentino
authors.initials: V
authors.lastname: Bezzerri
authors.forename: Concetta
authors.initials: C
authors.lastname: Avitabile
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Dechecchi
authors.forename: Ilaria
authors.initials: I
authors.lastname: Lampronti
authors.forename: Monica
authors.initials: M
authors.lastname: Borgatti
authors.forename: Giulia
authors.initials: G
authors.lastname: Montagner
authors.forename: Giulio
authors.initials: G
authors.lastname: Cabrini
authors.forename: Roberto
authors.initials: R
authors.lastname: Gambari
authors.forename: Alessandra
authors.initials: A
authors.lastname: Romanelli
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001980:Bronchi / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D002460:Cell Line; D002942:Circular Dichroism; D003550:Cystic Fibrosis / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D015195:Drug Design; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors / Q000378:metabolism; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D008968:Molecular Conformation; D011506:Proteins / Q000737:chemistry / Q000494:pharmacology; D011552:Pseudomonas Infections / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development / Q000276:immunology; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D013050:Spectrometry, Fluorescence
chemical_list: D000900:Anti-Bacterial Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D023181:Antimicrobial Cationic Peptides; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D011506:Proteins; C000598077:TB-KKG6A peptide; C104501:temporin
keywords: Pseudomonas aeruginosa; anti-inflammatory; antimicrobial; peptide; pro-inflammatory; temporin