title: Extremely 'vanadiphilic' multiply metal-resistant and halophilic aerobic anoxygenic phototrophs, strains EG13 and EG8, from hypersaline springs in Canada.
abstract: Two pinkish peach-colored strains of obligately aerobic phototrophic bacteria, EG13 and EG8, were isolated from a saline spring effluent stream in west central Manitoba, Canada. The strains possessed bacteriochlorophyll a incorporated into a typical purple bacterial light-harvesting complex 1 (870 nm) and reaction center (801 nm). Analysis of 16S rRNA gene sequences indicated 100% identity among the isolates and 99% similarity to Roseovarius tolerans EL-172(T). The strains were physiologically well adapted to high salinity (0-22%), fluctuating pH (7-12) and temperature (7-40 °C) of the exposed hypersaline stream of East German Creek. EG8 and EG13 were also highly resistant to the toxic metal(loid) oxyanions tellurite, selenite and metavanadate (≥1000 μg/ml each). Most intriguingly, growth and pigment production of EG13 on glutamate minimal medium was stimulated by 1000-10000 μg/ml of sodium metavanadate compared to metal-free conditions. Phylogenetic analysis and phenotypic properties such as pigment composition and morphology indicate close relatedness to Roseovarius genus.
authors.affiliation: Department of Microbiology, 418 Buller Building, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
authors.forename: J T
authors.initials: JT
authors.lastname: Csotonyi
authors.forename: C
authors.initials: C
authors.lastname: Maltman
authors.forename: J
authors.initials: J
authors.lastname: Swiderski
authors.forename: E
authors.initials: E
authors.lastname: Stackebrandt
authors.forename: V
authors.initials: V
authors.lastname: Yurkov
mesh_terms: D000332:Aerobiosis; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001429:Bacteriochlorophylls / Q000737:chemistry; D018533:Biomass; D002170:Canada; D004269:DNA, Bacterial / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000737:chemistry; D060985:Natural Springs / Q000382:microbiology*; D010788:Photosynthesis; D018524:Phototropism; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D013696:Temperature; D014639:Vanadium / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D001429:Bacteriochlorophylls; D004269:DNA, Bacterial; D008670:Metals; D012336:RNA, Ribosomal, 16S; D014639:Vanadium