title: Association study of miR‑149 rs2292832 and miR‑608 rs4919510 and the risk of hepatocellular carcinoma in a large‑scale population.
abstract: Polymorphisms in pre‑microRNAs (miRNAs) or mature miRNAs may influence miRNA processing or target binding, thus contributing to tumorigenesis and cancer development. The present study aimed to evaluate whether miR‑149 rs2292832 (C>T) and miR‑608 rs4919510 (G>C) are associated with the risk and clinical characteristics of hepatocellular carcinoma (HCC) in a large‑scale population. miR‑149 rs2292832 and miR‑608 rs4919510 were genotyped in a total of 993 patients with HCC and 992 unrelated healthy subjects by Sequenom MassARRAY. The results showed that, compared with the reference CC genotype, the TC+TT genotype of miR‑149 was more highly associated with HCC [CC vs. TC+TT: Odds ratio (OR)=1.384, 95% confidence interval (CI)=1.013‑1.892, P=0.041], and was also associated with an increased risk of hepatitis B virus (HBV)‑associated HCC (CC vs. TC+TT: OR=1.453, 95% CI=1.034‑2.042, P=0.031). However, no significant association between miRNA‑608 rs4919510 and the risk of HCC/HBV‑associated HCC was found. In addition, these two SNPs were shown not to be correlated with a range of clinical characteristics. The present study may provide an indicator for identification of the high risk of HCC in patients.
authors.affiliation: Department of Digestive Diseases, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Digestive Diseases, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Ministry of Education Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, P.R. China.
authors.forename: Yanyun
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Ministry of Education Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, P.R. China.
authors.forename: Linqi
authors.initials: L
authors.lastname: Chen
authors.affiliation: Ministry of Education Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, P.R. China.
authors.forename: Shicheng
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Digestive Diseases, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Digestive Diseases, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Yunfang
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Digestive Diseases, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Digestive Diseases, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Pei
authors.affiliation: Department of Biomedical Engineering, Northwestern University, Evanston, Illinois 60208, USA.
authors.forename: Siran
authors.initials: S
authors.lastname: Liu
authors.affiliation: Ministry of Education Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, P.R. China.
authors.forename: Jiucun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University, Shanghai 200438, P.R. China.
authors.forename: Heping
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Digestive Diseases, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012306:Risk
chemical_list: C551557:MIRN149 microRNA, human; C000591804:MIRN608 microRNA, human; D035683:MicroRNAs