title: Osmotic stress-induced phosphorylation of H2AX by polo-like kinase 3 affects cell cycle progression in human corneal epithelial cells.
abstract: Increased concentrations of extracellular solutes affect cell function and fate by stimulating cellular responses, such as evoking MAPK cascades, altering cell cycle progression, and causing apoptosis. Our study results here demonstrate that hyperosmotic stress induced H2AX phosphorylation (γH2AX) by an unrevealed kinase cascade involving polo-like kinase 3 (Plk3) in human corneal epithelial (HCE) cells. We found that hyperosmotic stress induced DNA-double strand breaks and increased γH2AX in HCE cells. Phosphorylation of H2AX at serine 139 was catalyzed by hyperosmotic stress-induced activation of Plk3. Plk3 directly interacted with H2AX and was colocalized with γH2AX in the nuclei of hyperosmotic stress-induced cells. Suppression of Plk3 activity by overexpression of a kinase-silencing mutant or by knocking down Plk3 mRNA effectively reduced γH2AX in hyperosmotic stress-induced cells. This was consistent with results that show γH2AX was markedly suppressed in the Plk3(-/-) knock-out mouse corneal epithelial layer in response to hyperosmotic stimulation. The effect of hyperosmotic stress-activated Plk3 and increased γH2AX in cell cycle progression showed an accumulation of G2/M phase, altered population in G1 and S phases, and increased apoptosis. Our results for the first time reveal that hyperosmotic stress-activated Plk3 elicited γH2AX. This Plk3-mediated activation of γH2AX subsequently regulates the cell cycle progression and cell fate.
authors.affiliation: From the Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Torrance, California 90502 and.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: the Department of Environmental Medicine, New York University School of Medicine, Tuxedo, New York 10987.
authors.forename: Wei
authors.initials: W
authors.lastname: Dai
authors.affiliation: From the Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Torrance, California 90502 and lluou@ucla.edu.
authors.forename: Luo
authors.initials: L
authors.lastname: Lu
mesh_terms: D000818:Animals; D002453:Cell Cycle; D053903:DNA Breaks, Double-Stranded; D004622:Embryo, Mammalian / Q000166:cytology; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism*; D019573:Epithelium, Corneal / Q000166:cytology*; D005347:Fibroblasts / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D006801:Humans; D018345:Mice, Knockout; D009997:Osmotic Pressure; D010766:Phosphorylation; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D025521:Tumor Suppressor Proteins; D000097868:Polo-like Kinases
chemical_list: D006657:Histones; D017346:Protein Serine-Threonine Kinases; D025521:Tumor Suppressor Proteins; C468783:H2AX protein, human; C486256:PLK3 protein, human; D000097868:Polo-like Kinases
keywords: Cell Cycle; Cornea; Epithelial Cell; H2A Histone Family, Member X (H2AFX); Phosphorylation; Signaling