title: Antisickling activity of butyl stearate isolated from Ocimum basilicum (Lamiaceae).
abstract: OBJECTIVE
  To perform phytochemical analyses on the leaves of Ocimum basilicum L. (O. basilicum), to elucidate the structure of isolate and then perform the antisickling activity on the crude extract and on the isolate.
  
  
  METHODS
  The Emmel test performed on the acidified methanolic extract of this plant was used to evaluate the antisickling activity. The structure characterization of the active compound was performed using chromatographic techniques for the separation and the spectroscopic ones for structure elucidation (1H-NMR, 13C-NMR, COSY, HMBC).
  
  
  RESULTS
  The chemical screening on the crude extract revealed the presence of polyphenols (flavonoids, anthocyanins, leucoanthocyanins, tannins, quinones) alkaloids, saponins, triterpenoids and steroids. The obtained extract after evaporation yielded 34.50 g (11.5%) out of 300 g of powdered leaves of O. basilicum. The acidified methanolic extract and butyl stearate showed an interesting antisickling activity.
  
  
  CONCLUSIONS
  The acidified methanolic extract and butyl stearate from O. basilicum displayed a good antisickling activity. To the best of our knowledge, this is the first time to report the antisickling activity of this compound in this plant. The synthesized compound presented the same spectroscopic characteristics than the natural one and the antisickling activities of its derivatives are understudying.
authors.affiliation: Faculté des Sciences B.P. 190, Université de Kinshasa, Kinshasa XI, R.D. Congo.
authors.forename: Dorothée Dinangayi
authors.initials: DD
authors.lastname: Tshilanda
authors.affiliation: Faculté des Sciences B.P. 190, Université de Kinshasa, Kinshasa XI, R.D. Congo.
authors.forename: Pius Tshimankinda
authors.initials: PT
authors.lastname: Mpiana
authors.affiliation: Faculté des Sciences B.P. 190, Université de Kinshasa, Kinshasa XI, R.D. Congo.
authors.forename: Damase Nguwo Vele
authors.initials: DN
authors.lastname: Onyamboko
authors.affiliation: Faculté des Sciences B.P. 190, Université de Kinshasa, Kinshasa XI, R.D. Congo.
authors.forename: Blaise Mavinga
authors.initials: BM
authors.lastname: Mbala
authors.affiliation: Faculté des Sciences B.P. 190, Université de Kinshasa, Kinshasa XI, R.D. Congo.
authors.forename: Koto-Te-Nyiwa
authors.initials: KT
authors.lastname: Ngbolua
authors.affiliation: Faculté des Sciences B.P. 190, Université de Kinshasa, Kinshasa XI, R.D. Congo.
authors.forename: Damien Sha Tshibey
authors.initials: DS
authors.lastname: Tshibangu
authors.affiliation: Faculté des Sciences B.P. 190, Université de Kinshasa, Kinshasa XI, R.D. Congo.
authors.forename: Matthieu Kokengo
authors.initials: MK
authors.lastname: Bokolo
authors.affiliation: Faculté des Sciences B.P. 190, Université de Kinshasa, Kinshasa XI, R.D. Congo.
authors.forename: Kalulu Muzele
authors.initials: KM
authors.lastname: Taba
authors.affiliation: Institut Supérieur Pédagogique de la Gombe (ISP-Gombe), Kinshasa, R.D. Congo.
authors.forename: Teddy Kabeya
authors.initials: TK
authors.lastname: Kasonga
keywords: Antisickling plant; Butyl stearate; Ocimum basilicum; Sickle cell disease