title: Imaging lipid metabolism in live Caenorhabditis elegans using fingerprint vibrations.
abstract: Quantitation of lipid storage, unsaturation, and oxidation in live C. elegans has been a long-standing obstacle. The combination of hyperspectral stimulated Raman scattering imaging and multivariate analysis in the fingerprint vibration region represents a platform that allows the quantitative mapping of fat distribution, degree of fat unsaturation, lipid oxidation, and cholesterol storage in vivo in the whole worm. Our results reveal for the first time that lysosome-related organelles in intestinal cells are sites for storage of cholesterol in C. elegans.
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907 (USA).
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Delong
authors.initials: D
authors.lastname: Zhang
authors.forename: Micah Y
authors.initials: MY
authors.lastname: Belew
authors.forename: Heidi A
authors.initials: HA
authors.lastname: Tissenbaum
authors.forename: Ji-Xin
authors.initials: JX
authors.lastname: Cheng
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000737:chemistry*; D003878:Dermatoglyphics; D008055:Lipids / Q000737:chemistry*; D053858:Metabolic Networks and Pathways; D013057:Spectrum Analysis / Q000379:methods*; D014732:Vibration
chemical_list: D008055:Lipids
keywords: C. elegans; cell metabolism; lipids; stimulated Raman scattering; vibrational spectroscopy