title: Quantitative histopathological assessment of ocular surface squamous neoplasia using digital image analysis.
abstract: The aim of this retrospective pilot study was to evaluate the Aperio nuclear V9 algorithm as an image analysis tool to observe the histopathological changes of ocular surface squamous neoplasia (OSSN). A histopathological assessment, including the Ki-67 proliferative index (PI) of immunohistochemically-stained tumor conjunctiva (TC) and healthy conjunctiva (HC) tissues, was performed in six cases of OSSN. The Aperio V9 algorithm was applied to digital images of the tissue specimens to count the Ki-67 PI and to measure the nuclear area indices. This digital algorithm was validated using stereological and visual analysis methods. The visual scoring of Ki-67 PI ranged from 22 to 60% (mean, 38.5%), and from 5 to 20% (mean 9.5%) in TC and HC tissue, respectively. The computer-aided analysis, using the Aperio nuclear V9 algorithm, revealed that the Ki-67 PI ranged from 21.5 to 43.5% (mean, 33.6%), and from 1.9 to 21.0% (mean, 11.8%) in the TC and HC tissue, respectively. The stereological method demonstrated that the Ki-67 PI ranged from 30.1 to 51.5% (mean, 41.0%), and from 3.2 to 30.1% (mean, 15.1%) in the TC and HC tissues, respectively. The strongest association in the collinearity of regression analysis was observed between the Aperio nuclear V9 algorithm/stereological models in the TC tissue (r2=0.7; P=0.04) and the HC tissue (r2=0.7; P=0.03), and the visual/stereological models in the TC tissue (r2=0.7; P=0.04) and the visual/Aperio nuclear V9 algorithm models in the HC tissue (r2=0.7; P=0.04). A weak and statistically insignificant association was identified between the visual/Aperio nuclear V9 algorithm analysis in the TC tissue (r2=0.4; P=0.2) and the visual/stereological models in the HC tissue (r2=0.5; P=0.13). No significant difference was observed between the nuclear area of the TC (mean, 36.5 μm2) and HC (mean, 35.7 μm2; P=0.88) tissues. It was concluded that the Aperio nuclear V9 algorithm is a useful tool for the reliable analysis of histopathological changes of OSSN. The results of this computer-aided algorithm correlate strongly with the stereological method when assessing the Ki-67 PI.
authors.affiliation: Faculty of Medicine, Vilnius University, Vilnius LT-03101, Lithuania ; Centre of Eye Diseases, Vilnius University Hospital Santariškių Klinikos, Vilnius LT-08661, Lithuania.
authors.forename: Rimvydas
authors.initials: R
authors.lastname: Ašoklis
authors.affiliation: Faculty of Medicine, Vilnius University, Vilnius LT-03101, Lithuania ; Centre of Eye Diseases, Vilnius University Hospital Santariškių Klinikos, Vilnius LT-08661, Lithuania.
authors.forename: Aistė
authors.initials: A
authors.lastname: Kadziauskienė
authors.affiliation: Centre of Eye Diseases, Vilnius University Hospital Santariškių Klinikos, Vilnius LT-08661, Lithuania.
authors.forename: Rasa
authors.initials: R
authors.lastname: Paulavičienė
authors.affiliation: Faculty of Medicine, Vilnius University, Vilnius LT-03101, Lithuania ; National Center of Pathology, Vilnius University Hospital Santariškių Klinikos, Vilnius LT-08661, Lithuania.
authors.forename: Donatas
authors.initials: D
authors.lastname: Petroška
authors.affiliation: Faculty of Medicine, Vilnius University, Vilnius LT-03101, Lithuania ; National Center of Pathology, Vilnius University Hospital Santariškių Klinikos, Vilnius LT-08661, Lithuania.
authors.forename: Arvydas
authors.initials: A
authors.lastname: Laurinavičius
keywords: Ki-67; digital image analysis; ocular surface squamous neoplasia