title: Blood-letting punctures at twelve Jing-Well points of the hand can treat cerebral ischemia in a similar manner to mannitol.
abstract: A rat model of middle cerebral artery permanent occlusion was established using the modified Longa method. Successfully established model animals were treated by blood-letting puncture at twelve Jing-Well points of the hand, and/or by injecting mannitol into the caudal vein twice daily. Brain tissue was collected at 24, 48 and 72 hours after modeling, and blood was collected through the retinal vein before Evans blue was injected, approximately 1 hour prior to harvesting of brain tissue. Results showed that Evans blue leakage into brain tissue and serum nitric oxide synthase activity were significantly increased in model rats. Treatment with blood-letting punctures at twelve Jing-Well points of the hand and/or injection of mannitol into the caudal vein reduced the amount of Evans blue leakage into the brain tissue and serum nitric oxide synthase activity to varying degrees. There was no significant difference between single treatment and combined treatment. Experimental findings indicate that blood-letting punctures at twelve Jing-Well points of the hand can decrease blood-brain barrier permeability and serum nitric oxide synthase activity in rats following middle cerebral artery occlusion, and its effect is similar to that of mannitol injection alone and Jing-Well points plus mannitol injection.
authors.affiliation: Experimental Acupuncture Research Center, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Lu
authors.affiliation: Experimental Acupuncture Research Center, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Zelin
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Experimental Acupuncture Research Center, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Experimental Acupuncture Research Center, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Gao
authors.affiliation: Experimental Acupuncture Research Center, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Experimental Acupuncture Research Center, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Zhongzheng
authors.initials: Z
authors.lastname: Li
authors.affiliation: Third Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou 310005, Zhejiang Province, China.
authors.forename: Jianqiao
authors.initials: J
authors.lastname: Fang
keywords: Jing-Well points of hand; acupoint; blood-brain barrier; blood-letting; brain injury; cerebral edema; cerebral infarction; cerebral ischemia; grants-supported paper; mannitol; middle cerebral artery occlusion; neural regeneration; neuroprotection; neuroregeneration; nitric oxide synthase