title: Subcellular fractionation of rough microsomes.
abstract: When eukaryotic cells are homogenized, the rough endoplasmic reticula are converted into small vesicles, called rough microsomes. Strategies for the isolation of rough microsomes are introduced here, as are methods for evaluating the purity and intactness of an isolated rough microsomal fraction.
authors.affiliation: Department of Cell Biology, NYU Medical School, New York, New York 10016.
authors.forename: David D
authors.initials: DD
authors.lastname: Sabatini
mesh_terms: D000818:Animals; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D006801:Humans; D008861:Microsomes / Q000378:metabolism / Q000648:ultrastructure*; D021381:Protein Transport; D013347:Subcellular Fractions / Q000378:metabolism / Q000648:ultrastructure*