title: Ultra-fast liquid chromatography with tandem mass spectrometry determination of ochratoxin A in traditional Chinese medicines based on vortex-assisted solid-liquid microextraction and aptamer-affinity column clean-up.
abstract: A rapid, selective, and sensitive ultra-fast liquid chromatography with tandem mass spectrometry method was developed for the determination of ochratoxin A in traditional Chinese medicines based on vortex-assisted solid-liquid microextraction and aptamer-affinity column clean-up. Through optimizing the sample pretreatment procedures and chromatographic conditions, good linearity (r(2) ≥ 0.9993), low limit of detection (0.5-0.8 μg/kg), and satisfactory recovery (83.54-94.44%) expressed the good reliability and applicability of the established method in various traditional Chinese medicines. Moreover, the aptamer-affinity column, prepared in-house, showed an excellent feasibility owing to its specific identification of ochratoxin A in various kinds of selected traditional Chinese medicines. The maximum adsorption amount and applicability value were 188.96 ± 10.56 ng and 72.3%, respectively. The matrix effects were effectively eliminated, especially for m/z 404.2→358.0 of ochratoxin A. The application of the developed method for screening the natural contamination levels of ochratoxin A in 25 random traditional Chinese medicines on the market in China indicated that only eight samples were contaminated with low levels below the legal limit (5.0 μg/kg) set by the European Union. This study provided a preferred choice for the rapid and accurate monitoring of ochratoxin A in complex matrices.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China; School of Pharmacy, Jiangsu University, Jiangsu, China.
authors.forename: Xihui
authors.initials: X
authors.lastname: Yang
authors.forename: Yichen
authors.initials: Y
authors.lastname: Hu
authors.forename: Weijun
authors.initials: W
authors.lastname: Kong
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Chu
authors.forename: Meihua
authors.initials: M
authors.lastname: Yang
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.forename: Zhen
authors.initials: Z
authors.lastname: Ouyang
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004340:Drug Contamination; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D009793:Ochratoxins; C025589:ochratoxin A
keywords: Aptamer-affinity columns; Ochratoxin A; Traditional Chinese medicine; Ultra-fast liquid chromatography with tandem mass spectrometry; Vortex-assisted solid-liquid microextraction