title: Effect of GPIIb/IIIa inhibition with eptifibatide or tirofiban on the expression of cellular adhesion molecules on monocytes.
abstract: OBJECTIVE
  The aim of the present study was to investigate the effect of GPIIb/IIIa inhibition with eptifibatide and tirofiban on the expression of cellular adhesion molecules on monocytes at different temperatures.
  
  
  METHODS
  Circulation of blood from six volunteers was performed in an extracorporal circulation model at 36°C and 18°C for 30 min. The blood of each donor was prepared either with addition of eptifibatide or tirofiban, or was left untreated as control. CD54 and CD162 on monocytes was measured using flow cytometry.
  
  
  RESULTS
  Expression of CD11b was lower at 18°C compared to 36°C by 51% in the eptifibatide group (p=0.0043), by 29% in the tirofiban group (p=0.095) and by 34% in the control group (p=0.038). Expression of CD54 was not significantly different at 18°C compared to 36°C, neither with eptifibatide (p=0.29) nor tirofiban (p=0.48) nor in the control group (p=0.26). Expression of CD162 was lower at 18°C compared to 36°C by 40% using eptifibatide (p=0.0010), by 94% using tirofiban (p=0.0095) and by 34% in the control group (p=0.019). At 36°C and 18°C, no significant differences were found regarding the expression of CD11b, CD54 and CD162 between the eptifibatide-treated group, the tirofiban-treated group and the control group.
  
  
  CONCLUSIONS
  GPIIb/IIIa inhibition with eptifibatide or tirofiban seems to have no effect on the expression of CD11b, CD54 and CD162 on monocytes during normothermia or hypothermia. Our results show that the beneficial effect induced by hypothermia on the extracorporal circulation-associated alteration of leukocyte function, with decreased expression of CD11b and CD162, seems not to be affected by additional treatment with eptifibatide or tirofiban.
authors.affiliation: Pharmacy Department of the University Hospital of Heidelberg, Heidelberg, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Swoboda
authors.affiliation: Emergency Department, University Medical Center Mannheim, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany thomas.walter.med@umm.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Walter
authors.affiliation: First Department of Medicine (Cardiology), University Medical Center Mannheim, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
authors.forename: Siegfried
authors.initials: S
authors.lastname: Lang
authors.affiliation: Clinic for Thoracic, Cardiac and Vascular Surgery, University of Tuebingen, Tuebingen, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Wendel
authors.affiliation: Department of Internal Medicine II, Kirchheim Hospital, Kirchheim, Germany.
authors.forename: Martin E
authors.initials: ME
authors.lastname: Beyer
authors.affiliation: Department of Internal Medicine II, Kirchheim Hospital, Kirchheim, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Griesel
authors.affiliation: Department of Internal Medicine II, Solingen Hospital, Solingen, Germany.
authors.forename: Hans-Martin
authors.initials: HM
authors.lastname: Hoffmeister
authors.affiliation: Emergency Department, University Medical Center Mannheim, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Gruettner
mesh_terms: D000328:Adult; D039481:CD11b Antigen / Q000235:genetics / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D000077542:Eptifibatide; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D064368:Healthy Volunteers; D006801:Humans; D016130:Immunophenotyping; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology*; D010975:Platelet Aggregation Inhibitors / Q000493:pharmacokinetics*; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000037:antagonists & inhibitors*; D000077466:Tirofiban; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D055815:Young Adult
chemical_list: D039481:CD11b Antigen; D015815:Cell Adhesion Molecules; D008562:Membrane Glycoproteins; C084882:P-selectin ligand protein; D010455:Peptides; D010975:Platelet Aggregation Inhibitors; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D018799:Intercellular Adhesion Molecule-1; D014443:Tyrosine; D000077466:Tirofiban; D000077542:Eptifibatide
keywords: CD11b; CD162; CD54; GPIIb/IIIa; eptifibatide; hypothermia; tirofiban