title: Modulation of Cerrena unicolor laccase and manganese peroxidase production.
abstract: Among seven carbon sources tested, glycerol and glucose favored the Cerrena unicolor laccase production (18.8-20.3 U/mL); in addition, glycerol ensured the highest manganese peroxidase (MnP) activity (2 U/mL). Substitution of glycerol with the ethanol production residue (EPR) gave the highest laccase (90.1 U/mL) activity, while the walnut pericarp provided the highest MnP activity (7.4 U/mL). Supplementation of medium with 1 mM copper and 1 mM xylidine at appropriate time caused significant additive effect on laccase expression (333.2 U/mL) in shake-flask experiments. Overproduction of laccase activity (507 U/mL) and secretion of MnP activity was obtained when C. unicolor was cultivated in stirred-tank fermenter. C. unicolor showed several distinctive and attractive technological features: it is capable to synthesize high levels of oxidases under high carbon and high nitrogen conditions and it secretes high laccase activity during trophophase.
authors.affiliation: Agricultural University of Georgia, 240 David Agmashenebeli alley, 0159 Tbilisi, Georgia.
authors.forename: Eva
authors.initials: E
authors.lastname: Kachlishvili
authors.affiliation: Agricultural University of Georgia, 240 David Agmashenebeli alley, 0159 Tbilisi, Georgia.
authors.forename: Eka
authors.initials: E
authors.lastname: Metreveli
authors.affiliation: Agricultural University of Georgia, 240 David Agmashenebeli alley, 0159 Tbilisi, Georgia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Elisashvili
keywords: Carbon source; Cerrena unicolor; Inducers; Laccase; Lignocellulosic materials; Manganese peroxidase; Submerged fermentation