title: Visualization of the ocular pulse in the anterior chamber of the mouse eye in vivo using phase-sensitive optical coherence tomography.
abstract: We report on a phase-based method for accurately measuring the ocular pulse in the anterior chamber in vivo. Using phase-sensitive optical coherence tomography with optimized scanning protocols and equations for compensating bulk motion and environmental vibrations, a high sensitivity of 0.9 μm/s minimal velocity is demonstrated at a wide detection band of 0 to 380 Hz. The pulsatile relative motion between cornea and crystalline lens in rodents is visualized and quantified. The relative motion is most likely caused by respiration (1.6 Hz) and heartbeat (6.6 Hz). The velocity amplitude of the relative motion is 10.3 ± 2.4 μm/s. The displacement amplitudes at the respiratory and cardiac frequencies are 202.5 ± 64.9 and 179.9 ± 49.4 nm, respectively. The potential applications the measurement technique can be found in the evaluation of intraocular pressure and the measurement of biomechanical properties of the ocular tissue, which are important in several ocular diseases.
authors.affiliation: Zhejiang University, State Key Lab of Modern Optical Instrumentation, Department of Optical Engineering, Hangzhou, Zhejiang 310027, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: Zhejiang University, State Key Lab of Modern Optical Instrumentation, Department of Optical Engineering, Hangzhou, Zhejiang 310027, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Ding
authors.affiliation: Zhejiang University, State Key Lab of Modern Optical Instrumentation, Department of Optical Engineering, Hangzhou, Zhejiang 310027, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Ni
authors.affiliation: Zhejiang University, College of Medicine, First Affiliated Hospital, Department of Ophthalmology, Hangzhou, Zhejiang 310027, China.
authors.forename: Baishen
authors.initials: B
authors.lastname: Xu
authors.affiliation: Zhejiang University, State Key Lab of Modern Optical Instrumentation, Department of Optical Engineering, Hangzhou, Zhejiang 310027, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Zhejiang University, State Key Lab of Modern Optical Instrumentation, Department of Optical Engineering, Hangzhou, Zhejiang 310027, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Zhejiang University, College of Medicine, First Affiliated Hospital, Department of Ophthalmology, Hangzhou, Zhejiang 310027, China.
authors.forename: Chixin
authors.initials: C
authors.lastname: Du
authors.affiliation: Zhejiang University, College of Medicine, First Affiliated Hospital, Department of Ophthalmology, Hangzhou, Zhejiang 310027, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000098:blood supply* / Q000502:physiology; D006339:Heart Rate / Q000502:physiology*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051379:Mice; D011674:Pulse / Q000379:methods*; D012815:Signal Processing, Computer-Assisted; D041623:Tomography, Optical Coherence / Q000379:methods*