title: Primers for phylogeny reconstruction in Bignonieae (Bignoniaceae) using herbarium samples.
abstract: OBJECTIVE
  New primers were developed for Bignonieae to enable phylogenetic studies within this clade using herbarium samples. •
  
  
  RESULTS
  Internal primers were designed based on available sequences of the plastid ndhF gene and the rpl32-trnL intergenic spacer region, and the nuclear gene PepC. The resulting primers were used to amplify DNA extracted from herbarium materials. High-quality data were obtained from herbarium samples up to 53 yr old. •
  
  
  CONCLUSIONS
  The standardized methodology allows the inclusion of herbarium materials as alternative sources of DNA for phylogenetic studies in Bignonieae.
authors.affiliation: Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, Rua do Matão 277, 05508-090, São Paulo, São Paulo, Brazil.
authors.forename: Alexandre R
authors.initials: AR
authors.lastname: Zuntini
authors.affiliation: Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, Rua do Matão 277, 05508-090, São Paulo, São Paulo, Brazil.
authors.forename: Luiz Henrique M
authors.initials: LH
authors.lastname: Fonseca
authors.affiliation: Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, Rua do Matão 277, 05508-090, São Paulo, São Paulo, Brazil.
authors.forename: Lúcia G
authors.initials: LG
authors.lastname: Lohmann
keywords: Bignonieae; PepC; herbarium specimens; ndhF; phylogeny; rpl32-trnL spacer