title: Hepatoprotective activity of twelve novel 7'-hydroxy lignan glucosides from Arctii Fructus.
abstract: Twelve novel 7'-hydroxy lignan glucosides (1-12), including two benzofuran-type neolignans, two 8-O-4' neolignans, two dibenzylbutyrolactone lignans, and six tetrahydrofuranoid lignans, together with six known lignan glucosides (13-18), were isolated from the fruit of Arctium lappa L. (Asteraceae), commonly known as Arctii Fructus. Their structures were elucidated using spectroscopy (1D and 2D NMR, MS, IR, ORD, and UV) and on the basis of chemical evidence. The absolute configurations of compounds 1-12 were confirmed using rotating frame nuclear overhauser effect spectroscopy (ROESY), the circular dichroic (CD) exciton chirality method, and Rh2(OCOCF3)4-induced CD spectrum analysis. All of the isolated compounds were tested for hepatoprotective effects against D-galactosamine-induced cytotoxicity in HL-7702 hepatic cells. Compounds 1, 2, 7-12, and 17 showed significantly stronger hepatoprotective activity than the positive control bicyclol at a concentration of 1 × 10(-5) M.
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing 100050, People's Republic of China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Yang
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Huang
authors.forename: Zi-Ming
authors.initials: ZM
authors.lastname: Feng
authors.forename: Jian-Shuang
authors.initials: JS
authors.lastname: Jiang
authors.forename: Pei-Cheng
authors.initials: PC
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D031170:Arctium / Q000737:chemistry*; D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D002942:Circular Dichroism; D004365:Drugs, Chinese Herbal; D005638:Fruit / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008113:Liver Neoplasms; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure
chemical_list: D004365:Drugs, Chinese Herbal; D005960:Glucosides; D017705:Lignans
keywords: Arctii Fructus; Arctium lappa L.; health food; hepatoprotective activity; isolation; lignan