title: Nitrogen removal and microbial characteristics in CANON biofilters fed with different ammonia levels.
abstract: The nitrogen removal performance and microbial characteristics of four completely autotrophic nitrogen removal over nitrite (CANON) biofilters were investigated. These four reactors were simultaneously seeded from a stable CANON biofilter with a seeding ratio of 1:1, which were fed with different ammonia levels. Results suggested that with the ammonia of 200-400 mg L(-1), aerobic ammonia-oxidizing bacteria (AerAOB) and anaerobic ammonia-oxidizing bacteria (AnAOB) could perform harmonious work. The bioactivity and population of the two groups of bacteria were both high, which then resulted in excellent nitrogen removal, while too low or too high ammonia would both lead to worse performance. When ammonia was too high, the bioactivity, biodiversity and population of AerAOB all decreased and then resulted in the lowest nitrogen removal. Nitrosomonas and Candidatus Brocadia were detected as predominant functional microbes in all the four reactors. Finally, strategies for treating sewage with different ammonia levels were proposed.
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China. Electronic address: lidong2006@bjut.edu.cn.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Huiping
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Shaoming
authors.initials: S
authors.lastname: Cui
authors.affiliation: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000641:Ammonia / Q000032:analysis*; D044822:Biodiversity; D019149:Bioreactors / Q000382:microbiology*; D017931:DNA Primers / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D058440:Denitrification / Q000502:physiology*; D005374:Filtration / Q000379:methods*; D005434:Flow Cytometry; D009606:Nitrosomonas / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D013054:Spectrophotometry, Atomic; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D017931:DNA Primers; D000641:Ammonia
keywords: Ammonia; Biodiversity; CANON; DGGE; FISH