title: Indoor air pollution from solid fuels and peripheral blood DNA methylation: findings from a population study in Warsaw, Poland.
abstract: DNA methylation is a potential mechanism linking indoor air pollution to adverse health effects. Fetal and early-life environmental exposures have been associated with altered DNA methylation and play a critical role in progress of diseases in adulthood. We investigated whether exposure to indoor air pollution from solid fuels at different lifetime periods was associated with global DNA methylation and methylation at the IFG2/H19 imprinting control region (ICR) in a population-based sample of non-smoking women from Warsaw, Poland. Global methylation and IFG2/H19 ICR methylation were assessed in peripheral blood DNA from 42 non-smoking women with Luminometric Methylation Assay (LUMA) and quantitative pyrosequencing, respectively. Linear regression models were applied to estimate associations between indoor air pollution and DNA methylation in the blood. Compared to women without exposure, the levels of LUMA methylation for women who had ever exposed to both coal and wood were reduced 6.70% (95% CI: -13.36, -0.04). Using both coal and wood before age 20 was associated with 6.95% decreased LUMA methylation (95% CI: -13.79, -0.11). Further, the negative correlations were more significant with exposure to solid fuels for cooking before age 20. There were no clear associations between indoor solid fuels exposure before age 20 and through the lifetime and IFG2/H19 ICR methylation. Our study of non-smoking women supports the hypothesis that exposure to indoor air pollution from solid fuels, even early-life exposure, has the capacity to modify DNA methylation that can be detected in peripheral blood.
authors.affiliation: Institute for Translational Epidemiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States; Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States; Department of Biostatistics and Epidemiology, University of North Texas Health Science Center, Fort Worth, TX 76107, United States. Electronic address: menghua.tao@unthsc.edu.
authors.forename: Meng-Hua
authors.initials: MH
authors.lastname: Tao
authors.affiliation: Institute for Translational Epidemiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States; Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States.
authors.forename: Jiachen
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Community and Preventive Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States.
authors.forename: Alexander P
authors.initials: AP
authors.lastname: Rialdi
authors.affiliation: Department of Biochemistry, Baylor University, Waco, TX 76798, United States.
authors.forename: Regina
authors.initials: R
authors.lastname: Martinez
authors.affiliation: Department of Cancer Epidemiology and Prevention, Cancer Center and M. Sklodowska-Curie Memorial Institute of Oncology, Warsaw 02781, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Dabek
authors.affiliation: Section of Genetic Epidemiology, International Agency for Research on Cancer, Lyon 69372, France.
authors.forename: Ghislaine
authors.initials: G
authors.lastname: Scelo
authors.affiliation: Department of Cancer Epidemiology and Prevention, Cancer Center and M. Sklodowska-Curie Memorial Institute of Oncology, Warsaw 02781, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Lissowska
authors.affiliation: Department of Community and Preventive Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States; Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States; Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States; Ruttenberg Cancer Center, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States.
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.affiliation: Institute for Translational Epidemiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States; Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States.
authors.forename: Paolo
authors.initials: P
authors.lastname: Boffetta
mesh_terms: D000368:Aged; D016902:Air Pollution, Indoor; D001483:Base Sequence; D003031:Coal; D019175:DNA Methylation; D017931:DNA Primers; D006801:Humans; D008297:Male; D008875:Middle Aged; D011044:Poland; D016133:Polymerase Chain Reaction; D012906:Smoke / Q000009:adverse effects; D014934:Wood
chemical_list: D003031:Coal; D017931:DNA Primers; D012906:Smoke
keywords: Epidemiology; Gene-specific methylation; Global methylation; IGF2/H19; Indoor air pollution