title: Identification of human linear B-cell epitope sites on the envelope glycoproteins of Crimean-Congo haemorrhagic fever virus.
abstract: A peptide library was used to screen for regions containing potential linear B-cell epitope sites in the glycoproteins and nucleoprotein of Crimean-Congo haemorrhagic fever virus (CCHFV) in an enzyme-linked immunosorbent assay (ELISA). The library consisted of 156 peptides, spanning the nucleoprotein and mature GN and GC proteins in a 19-mer with 9-mer overlap format. Using pooled serum samples from convalescent patients to screen the library, six peptides were identified as potential epitope sites. Further testing of these six peptides with individual patient sera identified two of these peptides as probable epitope sites, with peptide G1451-1469 reacting to 13/15 and peptide G1613-1631 to 14/15 human sera. These peptides are situated on the GC protein at amino acid positions 1451-1469 (relative to CCHFV isolate SPU103/97) (TCTGCYACSSGISCKVRIH) and 1613-1631 (FMFGWRILFCFKCCRRTRG). Identified peptides may have application in ELISA for diagnostic or serosurveillance purposes.
authors.affiliation: Department of Medical Microbiology and Virology,National Health Laboratory Service/University of the Free State,Bloemfontein,South Africa.
authors.forename: D
authors.initials: D
authors.lastname: Goedhals
authors.affiliation: Center for Emerging and Zoonotic Pathogens, National Institute for Communicable Diseases,National Health Laboratory Service,Johannesburg,South Africa.
authors.forename: J T
authors.initials: JT
authors.lastname: Paweska
authors.affiliation: Department of Medical Microbiology and Virology,National Health Laboratory Service/University of the Free State,Bloemfontein,South Africa.
authors.forename: F J
authors.initials: FJ
authors.lastname: Burt
mesh_terms: D000595:Amino Acid Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte / Q000235:genetics* / Q000378:metabolism; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D018985:Epitopes, B-Lymphocyte; D009698:Nucleoproteins; D014759:Viral Envelope Proteins
keywords: viral haemorrhagic fever