title: Impaired degranulation and proliferative capacity of Mycobacterium tuberculosis-specific CD8+ T cells in HIV-infected individuals with latent tuberculosis.
abstract: Human immunodeficiency virus (HIV)-infected individuals with latent Mycobacterium tuberculosis infection have substantially higher rates of progression to active tuberculosis than HIV-uninfected individuals with latent tuberculosis. To explore HIV-induced deficits in M. tuberculosis-specific CD8+ T-cell functions, we compared interferon γ production, degranulation, and proliferation of CD8+ T cells in response to M. tuberculosis peptides (ESAT-6/CFP-10) between HIV-infected (median CD4+ T-cell count, 522 cells/µL; interquartile range, 318-585 cells/µL) and HIV-uninfected individuals with latent tuberculosis from South Africa. We found that M. tuberculosis-specific degranulation and proliferative capacities were impaired in the HIV-infected group. Thus, our results suggest that HIV coinfection compromises CD8+ T-cell functions in latent tuberculosis.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine Department of Global Health, Rollins School of Public Health.
authors.forename: Ameeta S
authors.initials: AS
authors.lastname: Kalokhe
authors.affiliation: Emory Vaccine Center.
authors.forename: Toidi
authors.initials: T
authors.lastname: Adekambi
authors.affiliation: Emory Vaccine Center Department of Microbiology and Immunology, Yerkes National Primate Center, Emory University, Atlanta, Georgia.
authors.forename: Chris C
authors.initials: CC
authors.lastname: Ibegbu
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine.
authors.forename: Susan M
authors.initials: SM
authors.lastname: Ray
authors.affiliation: Department of Global Health, Rollins School of Public Health Emory Vaccine Center Department of Microbiology and Immunology, Yerkes National Primate Center, Emory University, Atlanta, Georgia.
authors.forename: Cheryl L
authors.initials: CL
authors.lastname: Day
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine Emory Vaccine Center.
authors.forename: Jyothi
authors.initials: J
authors.lastname: Rengarajan
mesh_terms: D000328:Adult; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000502:physiology; D015550:Cell Degranulation; D049109:Cell Proliferation; D002478:Cells, Cultured; D060085:Coinfection / Q000453:epidemiology / Q000276:immunology / Q000503:physiopathology; D005260:Female; D015658:HIV Infections / Q000150:complications* / Q000453:epidemiology / Q000503:physiopathology*; D006801:Humans; D055985:Latent Tuberculosis / Q000150:complications* / Q000453:epidemiology / Q000276:immunology / Q000503:physiopathology*; D007963:Leukocytes, Mononuclear; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology; D055815:Young Adult
keywords: CD8+ T cells; HIV; degranulation; latent tuberculosis; proliferation