title: Identification of differentially expressed genes in Chrysanthemum nankingense (Asteraceae) under heat stress by RNA Seq.
abstract: The RNA-Seq platform was used to characterize the high-temperature stress response of Chrysanthemum nankingense. A set of 54,668 differentially expressed unigenes was identified. After a threshold of ratio change ≥ 2 and a q-value of <0.05 were applied, the number of differentially transcribed genes was reduced to 3955, of which 765 were up-regulated and 3190 were down-regulated in response to heat stress. The differentially transcribed genes were predicted to participate in 26 biological processes, 4 cellular components, and 13 molecular functions. Among the most differentially expressed genes between the two libraries were well-recognized high-temperature responsive protein families, such as heat shock factors and heat shock proteins, various transcription factor families, and a number of RNA metabolism-related genes. Overall, the RNA-Seq analyses revealed a high degree of transcriptional complexity in early heat stress response. Some of these high-temperature responsive C. nankingense genes may prove useful in efforts to improve thermotolerance of commercial chrysanthemum.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: swxsunjing@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2012204032@njau.edu.cn.
authors.forename: Liping
authors.initials: L
authors.lastname: Ren
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2011104119@njau.edu.cn.
authors.forename: Yue
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2012204031@njau.edu.cn.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Gao
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2013204035@njau.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Dong
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: chensm@njau.edu.cn.
authors.forename: Sumei
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: chenfd@njau.edu.cn.
authors.forename: Fadi
authors.initials: F
authors.lastname: Chen
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: jiangjiafu@njau.edu.cn.
authors.forename: Jiafu
authors.initials: J
authors.lastname: Jiang
mesh_terms: D031202:Chrysanthemum / Q000235:genetics*; D015536:Down-Regulation; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics; D018869:Heat-Shock Response / Q000235:genetics*; D006358:Hot Temperature; D012313:RNA / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics; D059467:Transcriptome / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D006360:Heat-Shock Proteins; D014157:Transcription Factors; D012313:RNA
keywords: Chrysanthemum nankingense; Heat stress; RNA-Seq; Stress response