title: Design and development of a robotized system coupled to µCT imaging for intratumoral drug evaluation in a HCC mouse model.
abstract: Hepatocellular carcinoma (HCC) is one of the most common cancer related deaths worldwide. One of the main challenges in cancer treatment is drug delivery to target cancer cells specifically. Preclinical evaluation of intratumoral drugs in orthotopic liver cancer mouse models is difficult, as percutaneous injection hardly can be precisely performed manually. In the present study we have characterized a hepatoma model developing a single tumor nodule by implantation of Hep55.1C cells in the liver of syngeneic C57BL/6J mice. Tumor evolution was followed up by µCT imaging, and at the histological and molecular levels. This orthotopic, poorly differentiated mouse HCC model expressing fibrosis, inflammation and cancer markers was used to assess the efficacy of drugs. We took advantage of the high precision of a previously developed robotized system for automated, image-guided intratumoral needle insertion, to administer every week in the tumor of the Hep55.1C mouse model. A significant tumor growth inhibition was observed using our robotized system, whereas manual intraperitoneal administration had no effect, by comparison to untreated control mice.
authors.affiliation: Institut de Recherche contre les Cancers de l'Appareil Digestif (IRCAD), Strasbourg, France.
authors.forename: Gaétan
authors.initials: G
authors.lastname: Bour
authors.affiliation: IGBMC, Department of Functional Genomics and Cancer, CNRS/INSERM/Université de Strasbourg, BP 163, Illkirch, C. U. Strasbourg, Strasbourg, France.
authors.forename: Fernand
authors.initials: F
authors.lastname: Martel
authors.affiliation: ICube laboratory UMR, CNRS 7357, University of Strasbourg, Strasbourg, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Goffin
authors.affiliation: ICube laboratory UMR, CNRS 7357, University of Strasbourg, Strasbourg, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Bayle
authors.affiliation: ICube laboratory UMR, CNRS 7357, University of Strasbourg, Strasbourg, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Gangloff
authors.affiliation: Institut de Recherche contre les Cancers de l'Appareil Digestif (IRCAD), Strasbourg, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Aprahamian
authors.affiliation: Institut de Recherche contre les Cancers de l'Appareil Digestif (IRCAD), Strasbourg, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Marescaux
authors.affiliation: Institut de Recherche contre les Cancers de l'Appareil Digestif (IRCAD), Strasbourg, France; IGBMC, Department of Functional Genomics and Cancer, CNRS/INSERM/Université de Strasbourg, BP 163, Illkirch, C. U. Strasbourg, Strasbourg, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Egly
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000000981:diagnostic imaging / Q000188:drug therapy / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D004317:Doxorubicin / Q000494:pharmacology / Q000627:therapeutic use; D004341:Drug Evaluation / Q000295:instrumentation*; D008113:Liver Neoplasms / Q000000981:diagnostic imaging / Q000188:drug therapy / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D012371:Robotics; D055114:X-Ray Microtomography; D023041:Xenograft Model Antitumor Assays
chemical_list: D004317:Doxorubicin