title: Expression, purification, crystallization and preliminary X-ray analysis of glucose-1-phosphate uridylyltransferase (GalU) from Erwinia amylovora.
abstract: Glucose-1-phosphate uridylyltransferase from Erwinia amylovora CFPB1430 was expressed as a His-tag fusion protein in Escherichia coli. After tag removal, the purified protein was crystallized from 100 mM Tris pH 8.5, 2 M ammonium sulfate, 5% ethylene glycol. Diffraction data sets were collected to a maximum resolution of 2.46 Å using synchrotron radiation. The crystals belonged to the hexagonal space group P62, with unit-cell parameters a = 80.67, b = 80.67, c = 169.18. The structure was solved by molecular replacement using the structure of the E. coli enzyme as a search model.
authors.affiliation: Laboratory of Bioorganic Chemistry and Bio-Crystallography (B2Cl), Faculty of Science and Technology, Free University of Bolzano, Piazza Università 5, 39100 Bolzano, Italy.
authors.forename: Mirco
authors.initials: M
authors.lastname: Toccafondi
authors.affiliation: EMBL, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Michele
authors.initials: M
authors.lastname: Cianci
authors.affiliation: Laboratory of Bioorganic Chemistry and Bio-Crystallography (B2Cl), Faculty of Science and Technology, Free University of Bolzano, Piazza Università 5, 39100 Bolzano, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Benini
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray / Q000379:methods*; D017931:DNA Primers; D044084:Erwinia amylovora / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D017931:DNA Primers; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase
keywords: Erwinia; GalU; UDP-glucose; amylovoran; sugar metabolism; uridylyltransferase