title: Anomalous altered expressions of downstream gene-targets in TP53-miRNA pathways in head and neck cancer.
abstract: The prevalence of head and neck squamous cell carcinoma, HNSCC, continues to grow. Change in the expression of TP53 in HNSCC affects its downstream miRNAs and their gene targets, anomalously altering the expressions of the five genes, MEIS1, AGTR1, DTL, TYMS and BAK1. These expression alterations follow the repression of TP53 that upregulates miRNA-107, miRNA- 215, miRNA-34 b/c and miRNA-125b, but downregulates miRNA-155. The above five so far unreported genes are the targets of these miRNAs. Meta-analyses of microarray and RNA-Seq data followed by qRT-PCR validation unravel these new ones in HNSCC. The regulatory roles of TP53 on miRNA-155 and miRNA-125b differentiate the expressions of AGTR1 and BAK1in HNSCC vis-à-vis other carcinogenesis. Expression changes alter cell cycle regulation, angiogenic and blood cell formation, and apoptotic modes in affliction. Pathway analyses establish the resulting systems-level functional and mechanistic insights into the etiology of HNSCC.
authors.affiliation: Computational Biology Group, Indian Association for the Cultivation of Science, Kolkata, India.
authors.forename: Sanga
authors.initials: S
authors.lastname: Mitra
authors.affiliation: Department of Oncogene Regulation, Chittaranjan National Cancer Research Institute, Kolkata, India.
authors.forename: Nupur
authors.initials: N
authors.lastname: Mukherjee
authors.affiliation: Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, University of Gothenburg, Sweden.
authors.forename: Smarajit
authors.initials: S
authors.lastname: Das
authors.affiliation: Cancer Biology &Inflammatory Disorder Division, Indian Institute of Chemical Biology, Kolkata, India.
authors.forename: Pijush
authors.initials: P
authors.lastname: Das
authors.affiliation: Department of Oncogene Regulation, Chittaranjan National Cancer Research Institute, Kolkata, India.
authors.forename: Chinmay Kumar
authors.initials: CK
authors.lastname: Panda
authors.affiliation: 1] Computational Biology Group, Indian Association for the Cultivation of Science, Kolkata, India [2] Gyanxet, BF 286 Salt Lake, Kolkata, India.
authors.forename: Jayprokas
authors.initials: J
authors.lastname: Chakrabarti
mesh_terms: D063646:Carcinogenesis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D030541:Databases, Genetic; D016002:Discriminant Analysis; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics / Q000378:metabolism; D017423:Sequence Analysis, RNA; D000077195:Squamous Cell Carcinoma of Head and Neck; D060388:Support Vector Machine; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D050998:bcl-2 Homologous Antagonist-Killer Protein / Q000235:genetics / Q000378:metabolism
chemical_list: C544065:AGTR1 protein, human; C494772:BAK1 protein, human; D035683:MicroRNAs; D012333:RNA, Messenger; D044140:Receptor, Angiotensin, Type 1; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D050998:bcl-2 Homologous Antagonist-Killer Protein; D051057:Proto-Oncogene Proteins c-akt