title: Alleviation of Dermatophagoides microceras-induced allergy by an immunomodulatory protein, FIP-fve, from Flammulina velutipes in mice.
abstract: Asthma is a major public health concern. Its greatest risk factor is house dust mite (HDM). Dermatophagoides microceras (Der m) is a type of HDM, and in central Taiwan, there is approximately 80% prevalence of sensitization to Der m. FIP-fve is a fungal immunomodulatory protein (FIP) isolated from the fungus Flammulina velutipes, and exhibits anti-inflammatory properties. To investigate whether FIP-fve affects Der m-induced asthma and inflammation, we evaluated hyper-responsiveness (AHR), pathological changes, and cytokines in mice. We demonstrated that oral FIP-fve decreased Der m-induced airway AHR, airway inflammation, cell infiltration, and expression of cytokines in the bronchoalveolar lavage fluid of Balb/c mice. The results of this study suggest that FIP-fve suppresses asthma, inflammation, and respiratory pathogenesis stimulated by Der m. FIP-fve is able to maintain immunomodulatory activity even in simulated gastric fluid and intestinal fluid. FIP-fve could be a safe and stable agent for suppression of allergic asthma.
authors.affiliation: a Institute of Medicine , Chung Shan Medical University , Taichung , Taiwan.
authors.forename: Yu-Chi
authors.initials: YC
authors.lastname: Chang
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Hsiao
authors.forename: Shao-Chi
authors.initials: SC
authors.lastname: Hung
authors.forename: Ya-Wen
authors.initials: YW
authors.lastname: Chen
authors.forename: Chu-Chyn
authors.initials: CC
authors.lastname: Ou
authors.forename: Wei-Ting
authors.initials: WT
authors.lastname: Chang
authors.forename: Ko-Huang
authors.initials: KH
authors.lastname: Lue
authors.forename: Jiunn-Liang
authors.initials: JL
authors.lastname: Ko
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D005260:Female; D055393:Flammulina / Q000737:chemistry*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D005656:Fungal Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D006967:Hypersensitivity / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D015850:Interleukin-6 / Q000096:biosynthesis; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D020556:Neutrophil Infiltration / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D010991:Plethysmography; D055550:Protein Stability; D039981:Pyroglyphidae / Q000737:chemistry / Q000276:immunology; D012137:Respiratory System / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D000093242:Interleukin-6 Inhibitors
chemical_list: D000485:Allergens; D000894:Anti-Inflammatory Agents, Non-Steroidal; D005656:Fungal Proteins; D015850:Interleukin-6; D000093242:Interleukin-6 Inhibitors; C547132:FIP-fve protein, Flammulina velutipes
keywords: Der m (Dermatophagoides microceras); FIP-fve (fungal immunomodulatory protein-fve); asthma; house dust mite; immunomodulatory protein