title: Preliminary assessment of the prototype Yfiler® Plus kit in a population study of Northern Italian males.
abstract: A total of 150 samples of unrelated males from North of Italy were analyzed with the prototype Yfiler® Plus kit (Thermo Fisher Scientific, Oyster Point, CA). This kit is a short tandem repeat (STR) assay based on six-dye chemistry that amplifies 25 Y-STR loci. Sixteen loci are in common with Yfiler® kit and nine are new (DYS576, DYS627, DYS460, DYS518, DYS570, DYS449, DYS481, DYF387S1a/b, DYS533). In this population study, the improvement of adding additional Y-STR markers increased the discriminatory capacity from 0.787 with the minimum haplotype (MHT) loci to 1 with the prototype Yfiler® Plus kit.
authors.affiliation: Forensic Genetics Laboratory, Scientific Police Service, Department of the Minister of Interior, Rome, Italy.
authors.forename: Enrica
authors.initials: E
authors.lastname: Ottaviani
authors.forename: Stefano
authors.initials: S
authors.lastname: Vernarecci
authors.forename: Paola
authors.initials: P
authors.lastname: Asili
authors.forename: Alessandro
authors.initials: A
authors.lastname: Agostino
authors.forename: Paola
authors.initials: P
authors.lastname: Montagna
mesh_terms: D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting / Q000295:instrumentation*; D005787:Gene Frequency; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D007558:Italy; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000295:instrumentation*