title: Methylation in the promoters of HS3ST2 and CCNA1 genes is associated with cervical cancer in Uygur women in Xinjiang.
abstract: We assessed the suitability of HS3ST2 and CCNA1 genes as biomarkers for the early detection of cervical cancer in Uygur women in Xinjiang, China. Methylation-specific PCR (MSP) and HPV (HPV16 and HPV18)-specific PCR were performed on 110 cervical samples: 40 normal cervices, 10 cervical intraepithelial neoplasia 1 (CIN1), 10 CIN2, 10 CIN3 and 40 cervical cancer tissues. The expression of the 2 genes was measured by reverse transcription PCR (RT-PCR) in 10 methylation-positive and 10 methylation-negative cervical tissues. We found that both HS3ST2 and CCNA1 genes were methylated in 38 of the 40 cervical cancer tissues, 9 of the 10 CIN3, and 6 of the 10 CIN2. In contrast, methylation of these 2 genes was found in only 1 of the 40 normal tissues and none of 10 CIN1. Furthermore, hypermethylated HS3ST2 and CCNA1 genes were correlated with infection with HPV16 and HPV18 in high-grade squamous intraepithelial lesions (HSILs) and cervical cancer (both p<0.05). The expression of HS3ST2 and CCNA1 genes was lower in the methylation-positive cervical tissues than in the methylation-negative cervical tissues. Our results indicate that HS3ST2 and CCNA1 genes may play important roles in HPV-induced cervical cancer and that patients with specific hypermethylated genes may have a greater risk of progressing to invasive cervical cancer.
authors.affiliation: 1 Department of Biochemistry, School of Medicine, Shihezi University, Xinjiang Endemic and Ethnic Disease and Education Ministry Key Laboratory, Shihezi, Xinjiang - PR China.
authors.forename: Qiangqiang
authors.initials: Q
authors.lastname: Zuo
authors.forename: Weinan
authors.initials: W
authors.lastname: Zheng
authors.forename: Jinli
authors.initials: J
authors.lastname: Zhang
authors.forename: Zemin
authors.initials: Z
authors.lastname: Pan
authors.forename: Yinyin
authors.initials: Y
authors.lastname: Liu
authors.forename: Haichen
authors.initials: H
authors.lastname: Long
authors.forename: Peiwen
authors.initials: P
authors.lastname: Fan
authors.forename: Caili
authors.initials: C
authors.lastname: Guo
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Renfu
authors.initials: R
authors.lastname: Shao
mesh_terms: D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics; D002584:Cervix Uteri / Q000166:cytology; D002681:China; D056750:Cyclin A1 / Q000235:genetics*; D019175:DNA Methylation / Q000235:genetics*; D055088:Early Detection of Cancer; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics; D052161:Human papillomavirus 18 / Q000235:genetics; D006801:Humans; D030361:Papillomavirus Infections / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA; D065310:Squamous Intraepithelial Lesions of the Cervix / Q000235:genetics / Q000821:virology; D015238:Sulfotransferases / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; C503745:CCNA1 protein, human; D056750:Cyclin A1; D015238:Sulfotransferases; C021350:heparitin sulfotransferase