title: Sequential expression of cyclooxygenase-2, glutamate receptor-2, and platelet activating factor receptor in rat hippocampal neurons after fluid percussion injury.
abstract: Traumatic brain injury causes gene expression changes in different brain regions. Occurrence and development of traumatic brain injury are closely related, involving expression of three factors, namely cyclooxygenase-2, glutamate receptor-2, and platelet activating factor receptor. However, little is known about the correlation of these three factors and brain neuronal injury. In this study, primary cultured rat hippocampal neurons were subjected to fluid percussion injury according to Scott's method, with some modifications. RT-PCR and semi-quantitative immunocytochemical staining was used to measure the expression levels of cyclooxygenase-2, glutamate receptor-2, and platelet activating factor receptor. Our results found that cyclooxygenase-2 expression were firstly increased post-injury, and then decreased. Both mRNA and protein expression levels reached peaks at 8 and 12 hours post-injury, respectively. Similar sequential changes in glutamate receptor 2 were observed, with highest levels mRNA and protein expression at 8 and 12 hours post-injury respectively. On the contrary, the expressions of platelet activating factor receptor were firstly decreased post-injury, and then increased. Both mRNA and protein expression levels reached the lowest levels at 8 and 12 hours post-injury, respectively. Totally, our findings suggest that these three factors are involved in occurrence and development of hippocampal neuronal injury.
authors.affiliation: Second Department of Medicine, Inner Mongolia Corps Hospital, Chinese People's Armed Police Forces, Huhhot, Inner Mongolia Autonomous Region, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Pathology, General Hospital of Chinese People's Armed Police Forces, Beijing, China.
authors.forename: Qingming
authors.initials: Q
authors.lastname: Shu
authors.affiliation: Section of Pharmaceutical Chemistry, Department of Rescue Medicine, Logistics University of Chinese People's Armed Police Force, Tianjin, China.
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Li
authors.affiliation: Second Department of Surgery, Inner Mongolia Corps Hospital, Chinese People's Armed Police Forces, Huhhot, Inner Mongolia Autonomous Region, China.
authors.forename: Maolin
authors.initials: M
authors.lastname: Ge
authors.affiliation: Training Department, Logistics University of Chinese People's Armed Police Force, Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Tianjin, China.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Zhang
keywords: NSFC grant; RT-PCR; brain injury; cyclooxygenase-2; glutamate receptor 2; hippocampus; immunocytochemistry; nerve regeneration; neural regeneration; platelet activating factor; platelet activating factor receptor