title: Idarubicin-loaded folic acid conjugated magnetic nanoparticles as a targetable drug delivery system for breast cancer.
abstract: Conventional cancer chemotherapies cannot differentiate between healthy and cancer cells, and lead to severe side effects and systemic toxicity. Another major problem is the drug resistance development before or during the treatment. In the last decades, different kinds of controlled drug delivery systems have been developed to overcome these shortcomings. The studies aim targeted drug delivery to tumor site. Magnetic nanoparticles (MNP) are potentially important in cancer treatment since they can be targeted to tumor site by an externally applied magnetic field. In this study, MNPs were synthesized, covered with biocompatible polyethylene glycol (PEG) and conjugated with folic acid. Then, anti-cancer drug idarubicin was loaded onto the nanoparticles. Shape, size, crystal and chemical structures, and magnetic properties of synthesized nanoparticles were characterized. The characterization of synthesized nanoparticles was performed by dynamic light scattering (DLS), Fourier transform-infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), scanning electron microscopy (SEM) analyses. Internalization and accumulation of MNPs in MCF-7 cells were illustrated by light and confocal microscopy. Empty MNPs did not have any toxicity in the concentration ranges of 0-500μg/mL on MCF-7 cells, while drug-loaded nanoparticles led to significant toxicity in a concentration-dependent manner. Besides, idarubicin-loaded MNPs exhibited higher toxicity compared to free idarubicin. The results are promising for improvement in cancer chemotherapy.
authors.affiliation: Middle East Technical University, Department of Biological Sciences, Ankara 06800, Turkey; Middle East Technical University, Department of Biotechnology, Ankara 06800, Turkey. Electronic address: ufukg@metu.edu.tr.
authors.forename: Ufuk
authors.initials: U
authors.lastname: Gunduz
authors.affiliation: Middle East Technical University, Department of Biological Sciences, Ankara 06800, Turkey.
authors.forename: Tugba
authors.initials: T
authors.lastname: Keskin
authors.affiliation: Middle East Technical University, Department of Biological Sciences, Ankara 06800, Turkey.
authors.forename: Gulistan
authors.initials: G
authors.lastname: Tansık
authors.affiliation: Middle East Technical University, Central Laboratory, Molecular Biology and Biotechnology R&D Center, Ankara 06800, Turkey.
authors.forename: Pelin
authors.initials: P
authors.lastname: Mutlu
authors.affiliation: Ahi Evran University, Faculty of Engineering and Architecture, Kırsehir 40000, Turkey.
authors.forename: Serap
authors.initials: S
authors.lastname: Yalcin
authors.affiliation: Middle East Technical University, Department of Biotechnology, Ankara 06800, Turkey.
authors.forename: Gozde
authors.initials: G
authors.lastname: Unsoy
authors.affiliation: Afyon Kocatepe University, Department of Chemical Engineering, Afyon 03200, Turkey.
authors.forename: Arzu
authors.initials: A
authors.lastname: Yakar
authors.affiliation: Middle East Technical University, Department of Biotechnology, Ankara 06800, Turkey.
authors.forename: Rouhollah
authors.initials: R
authors.lastname: Khodadust
authors.affiliation: Middle East Technical University, Department of Chemical Engineering, Ankara 06800, Turkey.
authors.forename: Gungor
authors.initials: G
authors.lastname: Gunduz
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000378:metabolism; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D005492:Folic Acid / Q000008:administration & dosage* / Q000378:metabolism; D006801:Humans; D015255:Idarubicin / Q000008:administration & dosage* / Q000378:metabolism; D061986:MCF-7 Cells; D058185:Magnetite Nanoparticles / Q000008:administration & dosage*
chemical_list: D000970:Antineoplastic Agents; D058185:Magnetite Nanoparticles; D005492:Folic Acid; D015255:Idarubicin
keywords: Drug delivery; Folic acid; Idarubicin; MCF-7; Magnetic nanoparticle; Polyethylene glycol (PEG)