title: Effects of interleukin-10 polymorphisms and smoking on the risk of gastric cancer in Taiwan.
abstract: Gastric cancer is the second cause of death from cancer worldwide and its prevalence and mortality rates are still very high in developed countries. Interleukin-10 (IL10) is a pleiotropic cytokine produced by macrophages which can suppress and stimulate the immune response in tumorigenesis signaling. However, the contribution of IL10 genomic variants to gastric cancer is still largely unknown. In the present study, we aimed at investigating the role of IL10 genotypes in gastric cancer risk. The promoter single nucleotide polymorphisms on IL10, A-1082G (rs1800896), T-819C (rs3021097) and A-592C (rs1800872), were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method among 716 Taiwanese people (358 patients gastric cancer and 358 cancer-free controls). The results showed that there was a significant difference between the patient and control groups in the genotypic frequency distribution of IL10 A-1082G genotypes (p=0.0004). In addition, those carrying the G allele were found to have a higher risk for gastric cancer compared with those with the A allele (p=3.19×10(-5)). Furthermore, personal cigarrete smoking habits enhanced the gastric cancer risk for those IL10 A-1082G AG and GG carriers. In conclusion, AG and GG genotype at IL10 A-1082G, together with smoking, synergistically contribute to individual susceptibility for gastric cancer in Taiwan.
authors.affiliation: Division of Gastroenterology, Department of Internal Medicine, Kaoshiung Armed-Forces General Hospital, Kaoshiung, Taiwan, R.O.C. Division of Gastroenterology, Department of Internal Medicine, Taichung Armed-Forces General Hospital, Taichung, Taiwan, R.O.C. Department of Medicine, National Defense Medical Center, Taipei, Taiwan, R.O.C.
authors.forename: Wu-Hsien
authors.initials: WH
authors.lastname: Kuo
authors.affiliation: Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan, R.O.C. Department of Pharmacy, Taichung Armed-Forces General Hospital, Taichung, Taiwan, R.O.C.
authors.forename: Chung-Yu
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Division of Gastroenterology, Department of Internal Medicine, Kaoshiung Armed-Forces General Hospital, Kaoshiung, Taiwan, R.O.C.
authors.forename: Chun-Kai
authors.initials: CK
authors.lastname: Fu
authors.affiliation: Department of Pharmacy, Taichung Armed-Forces General Hospital, Taichung, Taiwan, R.O.C.
authors.forename: Yung-Hung
authors.initials: YH
authors.lastname: Hsieh
authors.affiliation: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors.forename: Cheng-Hsi
authors.initials: CH
authors.lastname: Liao
authors.affiliation: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors.forename: Chin-Mu
authors.initials: CM
authors.lastname: Hsu
authors.affiliation: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors.forename: Yi-Kai
authors.initials: YK
authors.lastname: Huang
authors.affiliation: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C. Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, R.O.C.
authors.forename: Chia-Wen
authors.initials: CW
authors.lastname: Tsai
authors.affiliation: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C. Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C.
authors.forename: Wen-Shin
authors.initials: WS
authors.lastname: Chang
authors.affiliation: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C. Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, R.O.C. Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C. artbau1@yahoo.com.tw artbau2@gmail.com.
authors.forename: Da-Tian
authors.initials: DT
authors.lastname: Bau
mesh_terms: D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012306:Risk; D012907:Smoking / Q000009:adverse effects*; D013274:Stomach Neoplasms / Q000453:epidemiology / Q000209:etiology*; D013624:Taiwan
chemical_list: D016753:Interleukin-10
keywords: Gastric cancer; IL10; genotype; polymorphism; smoking