title: Prognostic value of O-6-methylguanine-DNA methyltransferase loss in salivary gland carcinomas.
abstract: BACKGROUND
  The purpose of this study was to determine the prognostic value of O-6-methylguanine-DNA methyltransferase (MGMT) inactivation in a group of 286 patients with salivary gland carcinoma and matched histologically normal tissues.
  
  
  METHODS
  MGMT promoter methylation was studied in 36 patients with salivary gland carcinoma and 19 histologically matched normal tissues by pyrosequencing. MGMT protein expression was examined in 286 patients with salivary gland carcinomas and histologically matched normal tissues by immunohistochemistry on tissue microarrays. The results were correlated to demographic, clinicopathologic parameters, and disease follow-up data.
  
  
  RESULTS
  MGMT hypermethylation was significantly (p = .021) associated with the protein loss. MGMT loss was found in 39.2% of salivary gland carcinomas and was predominant in aggressive tumors (poorly differentiated, grade III, regional lymph node involved). MGMT loss significantly (p = .004) predicted poor clinical outcome of salivary gland carcinomas and defined high-risk subgroups in clinically favorable tumor groups.
  
  
  CONCLUSIONS
  We suggest that immunohistochemical evaluation of nuclear MGMT protein might serve as a tool for the prediction of overall survival in patients with salivary gland carcinoma.
authors.affiliation: Faculty of Natural Sciences, Vilnius University, Vilnius, Lithuania.
authors.forename: Asta
authors.initials: A
authors.lastname: Scesnaite
authors.forename: Sonata
authors.initials: S
authors.lastname: Jarmalaite
authors.forename: Maximillian
authors.initials: M
authors.lastname: Mueller
authors.forename: Abbas
authors.initials: A
authors.lastname: Agaimy
authors.forename: Johannes
authors.initials: J
authors.lastname: Zenk
authors.forename: Arndt
authors.initials: A
authors.lastname: Hartmann
authors.forename: Heinrich
authors.initials: H
authors.lastname: Iro
authors.forename: Brigitte
authors.initials: B
authors.lastname: Peters
authors.forename: Stephan
authors.initials: S
authors.lastname: Schwarz
authors.forename: Regine
authors.initials: R
authors.lastname: Schneider-Stock
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002277:Carcinoma / Q000201:enzymology* / Q000401:mortality / Q000473:pathology; D016022:Case-Control Studies; D002648:Child; D019175:DNA Methylation; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000378:metabolism*; D011237:Predictive Value of Tests; D011379:Prognosis; D011401:Promoter Regions, Genetic; D012468:Salivary Gland Neoplasms / Q000201:enzymology* / Q000401:mortality / Q000473:pathology; D055815:Young Adult
chemical_list: D019853:O(6)-Methylguanine-DNA Methyltransferase
keywords: O6-methylguanine-DNA methyltransferase; methylation; prognosis; protein expression; salivary gland carcinoma