title: Pantoea sp. isolated from tropical fresh water exhibiting N-acyl homoserine lactone production.
abstract: N-Acyl homoserine lactone (AHL) serves as signaling molecule for quorum sensing (QS) in Gram-negative bacteria to regulate various physiological activities including pathogenicity. With the aim of isolating freshwater-borne bacteria that can cause outbreak of disease in plants and portrayed QS properties, environmental water sampling was conducted. Here we report the preliminary screening of AHL production using Chromobacterium violaceum CV026 and Escherichia coli [pSB401] as AHL biosensors. The 16S rDNA gene sequence of isolate M009 showed the highest sequence similarity to Pantoea stewartii S9-116, which is a plant pathogen. The isolated Pantoea sp. was confirmed to produce N-3-oxohexanoyl-L-HSL (3-oxo-C6-HSL) through analysis of high resolution mass tandem mass spectrometry.
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Wen-Si
authors.initials: WS
authors.lastname: Tan
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Nina Yusrina
authors.identifier: 0000-0001-6525-4405
authors.initials: NY
authors.lastname: Muhamad Yunos
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Pui-Wan
authors.initials: PW
authors.lastname: Tan
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Nur Izzati
authors.identifier: 0000-0002-8166-4524
authors.initials: NI
authors.lastname: Mohamad
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Tan-Guan-Sheng
authors.initials: TG
authors.lastname: Adrian
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Wai-Fong
authors.initials: WF
authors.lastname: Yin
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Kok-Gan
authors.initials: KG
authors.lastname: Chan
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000096:biosynthesis; D001483:Base Sequence; D002861:Chromobacterium; D004926:Escherichia coli; D005618:Fresh Water / Q000382:microbiology*; D006714:Homoserine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D008296:Malaysia; D008969:Molecular Sequence Data; D020636:Pantoea / Q000235:genetics* / Q000378:metabolism*; D053038:Quorum Sensing / Q000502:physiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017423:Sequence Analysis, RNA; D017385:Sequence Homology; D053719:Tandem Mass Spectrometry; D014329:Tropical Climate
chemical_list: C109860:N-(3-oxododecanoyl)homoserine lactone; D012336:RNA, Ribosomal, 16S; D006714:Homoserine; D015107:4-Butyrolactone