title: Dosing-time dependent oxidative effects of sodium nitroprusside in brain, kidney, and liver of mice.
abstract: The purpose of this study was to investigate if the oxidative effects of sodium nitroprusside (SNP) are dosing-time dependent. Therefore, the variation of malondialdehyde (MDA) status was assessed after a single i.p. administration of SNP (2.5mgkg(-1) b.w.) or vehicle (9‰ NaCl) to different and comparable groups of mice (n=48) at two different circadian times (1 and 13h after light onset [HALO]). Brain, kidney, and liver tissues were excised over 36h, and their MDA contents were estimated at 0, 1, 3, 6, 9, 12, 24, and 36h after SNP administration.
  
  
  RESULTS
  indicated mean MDA level was not significantly changed in each investigated tissue compared with the control. In contrast, the mean MDA value varied among organs and was comparable in brain and liver but lower than in kidney. The data show SNP significantly (P<0.05) increases MDA status in both tissues and exerts time-dependent oxidative effects with the greatest toxicity coinciding with the beginning of the diurnal rest span (local time: 08:00h, i.e., at 1 HALO). The obtained results reveal SNP-induced oxidative damage (evidenced by MDA accumulation) varies according to both the dosing-time and the target organ.
authors.affiliation: UMR Biosurveillance et Toxicologie Environnementale, Département de Biologie, Faculté des Sciences et Techniques de Maradi, 465 Maradi, Niger. Electronic address: sanimamane@yahoo.fr.
authors.forename: Mamane
authors.initials: M
authors.lastname: Sani
authors.affiliation: UR Ethnobotanie et Stress Oxydant, Département des Sciences de la Vie, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisia.
authors.forename: Hichem
authors.initials: H
authors.lastname: Sebai
authors.affiliation: UR Ethnobotanie et Stress Oxydant, Département des Sciences de la Vie, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisia.
authors.forename: Néziha
authors.initials: N
authors.lastname: Ghanem-Boughanmi
authors.affiliation: Laboratoire de Pharmacologie, Faculté de Médecine, 5019 Monastir, Tunisia.
authors.forename: Naceur A
authors.initials: NA
authors.lastname: Boughattas
authors.affiliation: Laboratoire de Biosurveillance de l'Environnement, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisia.
authors.forename: Mossadok
authors.initials: M
authors.lastname: Ben-Attia
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects*; D051379:Mice; D020030:Nitric Oxide Donors / Q000633:toxicity*; D009599:Nitroprusside / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D013997:Time Factors
chemical_list: D020030:Nitric Oxide Donors; D009599:Nitroprusside
keywords: Brain; Kidney; Liver; Malondialdehyde; Mice; Sodium nitroprusside