title: Development of 11 microsatellite loci for an endangered herb, Paraisometrum mileense (Gesneriaceae), in southwest China.
abstract: OBJECTIVE
  Microsatellite markers were developed to investigate the genetic diversity and population structure of the endangered species Paraisometrum mileense, as well as its evolutionary history. •
  
  
  RESULTS
  Using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, 11 polymorphic primer sets were obtained in P. mileense. The number of alleles per locus ranged from one to nine with an average 3.8, and the observed and expected heterozygosities ranged from 0 to 1 and 0 to 0.89, respectively, for the available three populations. •
  
  
  CONCLUSIONS
  These markers may be useful for further investigation of the conservation genetics of P. mileense.
authors.affiliation: College of Life Sciences, Chongqing Normal University, Chongqing 401331, People's Republic of China.
authors.forename: An-Jun
authors.initials: AJ
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Ren-Chao
authors.initials: RC
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, People's Republic of China.
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Ge
authors.affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
keywords: Gesneriaceae; Paraisometrum mileense; endangered herb; microsatellite; population genetics