title: miR‑886‑3p upregulation in clear cell renal cell carcinoma regulates cell migration, proliferation and apoptosis by targeting PITX1.
abstract: miR‑886‑3p has been discovered to be involved in the oncogenesis, progression and metastasis of several types of human cancer. The aim of the present study was to identify the biological function of miR‑886‑3p in clear cell renal cell carcinoma (ccRCC) and to determine its possible molecular mechanisms. miR‑886‑3p was found to be significantly upregulated in ccRCC tissues (P<0.05), in accordance with a previous sequencing result. Functional experiments revealed that forced downregulation of miR‑886‑3p significantly inhibited cellular migration, suppressed cell proliferation and induced cell apoptosis of renal cancer cells. Paired‑like homeodomain 1 (PITX1), which has been identified as a tumor suppressor, was found to be downregulated in ccRCC tissues and identified as a target gene of miR‑886‑3p. Further experiments demonstrated that the protein level, and not the mRNA level, of PITX1 was significantly decreased or increased when miR‑886‑3p was upregulated or downregulated, respectively, indicating that miR‑886‑3p acted as an oncogene by directly regulating the protein expression of PITX1 at a post‑transcriptional level. In conclusion, this study revealed that miR‑886‑3p was upregulated in ccRCC and was involved in cellular migration, proliferation and apoptosis of renal cancer cells by directly targeting the tumor suppressor gene, PITX1.
authors.affiliation: Department of Urology, Peking University Shenzhen Hospital, Institute of Urology, Shenzhen PKU‑HKUST Medical Center, Shenzhen 518036, P.R. China.
authors.forename: Zuhu
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Department of Urology, Peking University Shenzhen Hospital, Institute of Urology, Shenzhen PKU‑HKUST Medical Center, Shenzhen 518036, P.R. China.
authors.forename: Duqun
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Urology, Peking University Shenzhen Hospital, Institute of Urology, Shenzhen PKU‑HKUST Medical Center, Shenzhen 518036, P.R. China.
authors.forename: Zhengming
authors.initials: Z
authors.lastname: Su
authors.affiliation: Department of Urology, Peking University Shenzhen Hospital, Institute of Urology, Shenzhen PKU‑HKUST Medical Center, Shenzhen 518036, P.R. China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Urology, Peking University Shenzhen Hospital, Institute of Urology, Shenzhen PKU‑HKUST Medical Center, Shenzhen 518036, P.R. China.
authors.forename: Wenshui
authors.initials: W
authors.lastname: Yu
authors.affiliation: Department of Urology, Peking University Shenzhen Hospital, Institute of Urology, Shenzhen PKU‑HKUST Medical Center, Shenzhen 518036, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: The Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Shenzhen PKU‑HKUST Medical Center, Shenzhen 518036, P.R. China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Yang
authors.affiliation: The Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Shenzhen PKU‑HKUST Medical Center, Shenzhen 518036, P.R. China.
authors.forename: Cailing
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Urology, Peking University Shenzhen Hospital, Institute of Urology, Shenzhen PKU‑HKUST Medical Center, Shenzhen 518036, P.R. China.
authors.forename: Shangqi
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Urology, Peking University Shenzhen Hospital, Institute of Urology, Shenzhen PKU‑HKUST Medical Center, Shenzhen 518036, P.R. China.
authors.forename: Liangchao
authors.initials: L
authors.lastname: Ni
authors.affiliation: The Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Shenzhen PKU‑HKUST Medical Center, Shenzhen 518036, P.R. China.
authors.forename: Yaoting
authors.initials: Y
authors.lastname: Gui
authors.affiliation: Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing 100083, P.R. China.
authors.forename: Zebin
authors.initials: Z
authors.lastname: Mao
authors.affiliation: Department of Urology, Peking University Shenzhen Hospital, Institute of Urology, Shenzhen PKU‑HKUST Medical Center, Shenzhen 518036, P.R. China.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Lai
mesh_terms: D017209:Apoptosis; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D002465:Cell Movement; D049109:Cell Proliferation; D019295:Computational Biology; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D051761:Paired Box Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: C559060:MIRN886 microRNA, human; D035683:MicroRNAs; D051761:Paired Box Transcription Factors; C101485:homeobox protein PITX1