title: Proteomic identification of dihydrolipoamide dehydrogenase as a target of autoantibodies in patients with endometrial cancer.
abstract: OBJECTIVE
  Accumulating evidence shows that various types of cancers induce a specific immune response, resulting in the production of antibodies against self-components (autoantibodies). The aim of the present study was to identify antigens for autoantibodies in sera from endometrial cancer patients as novel diagnostic markers for the disease.
  
  
  METHODS
  The reactivity of individual sera from patients was examined by 2-dimensional (2-D) immunoblotting using HeLa cell lysates as antigens to identify autoantigens. ELISA was established to quantitatively measure autoantibody titer of patients' sera.
  
  
  RESULTS
  A mitochondrial protein, dihydrolipoamide dehydrogenase (DLD), was identified as an autoantigen specific to endometrial cancer patients. The levels of immunoglobulin (Ig)A but not IgG autoantibody to DLD were significantly increased in the sera of endometrial cancer patients.
  
  
  CONCLUSIONS
  IgA autoantibody against DLD could be a novel diagnostic marker for endometrial cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Nippon Medical School Hospital, Tokyo, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Yoneyama
authors.affiliation: Division of Cellular Proteomics, Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
authors.forename: Rina
authors.initials: R
authors.lastname: Shibata
authors.affiliation: Department of Advanced Medicine and Development, BML Inc., Saitama, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Igarashi
authors.affiliation: Department of Advanced Medicine and Development, BML Inc., Saitama, Japan.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Kojima
authors.affiliation: Department of Advanced Medicine and Development, BML Inc., Saitama, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Kodani
authors.affiliation: Department of Advanced Medicine and Development, BML Inc., Saitama, Japan.
authors.forename: Kinya
authors.initials: K
authors.lastname: Nagata
authors.affiliation: Department of Obstetrics and Gynecology, Nippon Medical School Hospital, Tokyo, Japan.
authors.forename: Keisuku
authors.initials: K
authors.lastname: Kurose
authors.affiliation: Department of Obstetrics and Gynecology, Nippon Medical School Hospital, Tokyo, Japan.
authors.forename: Rieko
authors.initials: R
authors.lastname: Kawase
authors.affiliation: Department of Obstetrics and Gynecology, Nippon Medical School Hospital, Tokyo, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Takeshita
authors.affiliation: Division of Cellular Proteomics, Institute of Medical Science, The University of Tokyo, Tokyo, Japan Division of Biochemistry, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan hattoris@pharm.kitasato-u.ac.jp.
authors.forename: Seisuke
authors.initials: S
authors.lastname: Hattori
mesh_terms: D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000276:immunology; D016022:Case-Control Studies; D002460:Cell Line; D008058:Dihydrolipoamide Dehydrogenase / Q000276:immunology* / Q000378:metabolism; D016889:Endometrial Neoplasms / Q000175:diagnosis / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D008875:Middle Aged; D009367:Neoplasm Staging; D040901:Proteomics / Q000379:methods; D002583:Uterine Cervical Neoplasms / Q000276:immunology / Q000378:metabolism
chemical_list: D001323:Autoantibodies; D014408:Biomarkers, Tumor; D007070:Immunoglobulin A; D008058:Dihydrolipoamide Dehydrogenase
keywords: Autoantibody; biomarker; diagnosis; dihydrolipoamide dehydrogenase; endometrial cancer