title: microRNA-181 promotes prostate cancer cell proliferation by regulating DAX-1 expression.
abstract: microRNAs (miRNAs) are a class of short noncoding RNA molecules that have a critical role in the initiation and progression of types of human cancer, including prostate cancer. In the present study, the expression of miR-181 in prostate cancer tissues was evaluated and was demonstrated to be significantly upregulated in prostate cancer tissues compared with that in adjacent normal tissues. The results ofin vitroMTT and BrdU incorporation assays, as well as cell-cycle analysis, indicated that miR-181 overexpression markedly promoted the proliferation of LNCaP cells. Furthermore, miR-181 overexpression was found to promote the progression of LNCaP tumor growth in nude mice. Mechanistic studies demonstrated that dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (DAX-1), a negative regulator of androgen receptor in prostate cancer, was inhibited by miR-181 overexpression. Therefore, the results from the present study suggest that miR-181 functions as a growth-suppressive miRNA during prostate cancer development.
authors.affiliation: Department of Urologic Surgery, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, P.R. China.
authors.forename: Shi-Jun
authors.initials: SJ
authors.lastname: Tong
authors.affiliation: Department of Urologic Surgery, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Urologic Surgery, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, P.R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Urologic Surgery, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, P.R. China.
authors.forename: Lian-Xi
authors.initials: LX
authors.lastname: Qu
keywords: DAX-1; cell proliferation; miR-181; microRNA; prostate cancer