title: Production of polyhydroxyalkanoates (PHA) by bacterial consortium from excess sludge fermentation liquid at laboratory and pilot scales.
abstract: The generation of polyhydroxyalkanoates (PHA) from excess sludge fermentation liquid (SFL) was studied at lab and pilot scale. A PHA-accumulated bacterial consortium (S-150) was isolated from activated sludge using simulated SFL (S-SFL) contained high concentration volatile fatty acids (VFA) and nitrogen. The maximal PHA content accounted for 59.18% in S-SFL and dropped to 23.47% in actual SFL (L-SFL) of the dry cell weight (DCW) at lab scale. The pilot-scale integrated system comprised an anaerobic fermentation reactor (AFR), a ceramic membrane system (CMS) and a PHA production bio-reactor (PHAR). The PHA content from pilot-scale SFL (P-SFL) finally reached to 59.47% DCW with the maximal PHA yield coefficient (YP/S) of 0.17 g PHA/g COD. The results indicated that VFA-containing SFL was suitable for PHA production. The adverse impact of excess nitrogen and non-VFAs in SFL might be eliminated by pilot-scale domestication, which might resulted in community structure optimization and substrate selective ability improvement of S-150.
authors.affiliation: State Key Joint Laboratory on Environmental Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China; Beijing Municipal Institute of City Planning & Design, Beijing 100045, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Jia
authors.affiliation: State Key Joint Laboratory on Environmental Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China.
authors.forename: Huilei
authors.initials: H
authors.lastname: Xiong
authors.affiliation: State Key Joint Laboratory on Environmental Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China. Electronic address: wanghui@tsinghua.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Joint Laboratory on Environmental Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China.
authors.forename: Hanchang
authors.initials: H
authors.lastname: Shi
authors.affiliation: State Key Joint Laboratory on Environmental Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China; Environment Research Institute, Shandong University, Jinan 250100, China.
authors.forename: Xinying
authors.initials: X
authors.lastname: Sheng
authors.affiliation: State Key Joint Laboratory on Environmental Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China.
authors.forename: Run
authors.initials: R
authors.lastname: Sun
authors.affiliation: School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Chen
mesh_terms: D001421:Bacteria, Anaerobic / Q000378:metabolism*; D019149:Bioreactors; D005232:Fatty Acids, Volatile / Q000032:analysis; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D008567:Membranes, Artificial; D009584:Nitrogen / Q000032:analysis; D010865:Pilot Projects; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D012722:Sewage / Q000382:microbiology*
chemical_list: D005232:Fatty Acids, Volatile; D008567:Membranes, Artificial; D054813:Polyhydroxyalkanoates; D012722:Sewage; D009584:Nitrogen
keywords: Bacterial consortium; Pilot scale; Polyhydroxyalkanoates (PHA); Sludge fermentation liquid (SFL)