title: Differential expression of hemolymph proteins between susceptible and insecticide-resistant Blattella germanica (Blattodea: Blattellidae).
abstract: A proteomic approach combining two-dimensional polyacrylamide gel electrophoresis and tandem mass spectrometry was used to compare hemolymph expression profiles of a beta-cypermethrin-resistant Blattella germanica L. strain and a beta-cypermethrin-susceptible strain. Twenty-eight hemolymph proteins were differentially expressed in the resistant cockroach strain; 19 proteins were upregulated and 9 proteins were downregulated compared with the susceptible strain. Protein identification indicated that expression of putative cuticular protein, nitric oxide synthase, triosephosphate isomerase, alpha-amylase, ABC transporter, and Per a 3 allergen was elevated, and expression of arginine kinase and glycosidase was reduced. The differential expression of these proteins reflects the overall change in cellular structure and metabolism related to the resistance of pyrethroid insecticides.
authors.affiliation: Key Laboratory of Animal Resistance Research, College of Life Science, Shandong Normal University, 88 East Wenhua Rd., Jinan 250014, People's Republic of China.
authors.forename: F
authors.initials: F
authors.lastname: Zhang
authors.forename: X J
authors.initials: XJ
authors.lastname: Wang
authors.forename: Y H
authors.initials: YH
authors.lastname: Huang
authors.forename: Z G
authors.initials: ZG
authors.lastname: Zhao
authors.forename: S S
authors.initials: SS
authors.lastname: Zhang
authors.forename: X S
authors.initials: XS
authors.lastname: Gong
authors.forename: L
authors.initials: L
authors.lastname: Xie
authors.forename: D M
authors.initials: DM
authors.lastname: Kang
authors.forename: X
authors.initials: X
authors.lastname: Jing
mesh_terms: D000818:Animals; D020048:Blattellidae / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation; D006458:Hemolymph / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D008297:Male; D020543:Proteome; D011722:Pyrethrins / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D020543:Proteome; D011722:Pyrethrins; C017160:cypermethrin