title: New microsatellite markers for Tricyrtis macrantha (Convallariaceae) and cross-amplification in closely related species.
abstract: OBJECTIVE
  Microsatellite markers were characterized in Tricyrtis macrantha (sect. Brachycyrtis, Convallariaceae), a vulnerable species endemic to Japan, to investigate its genetic diversity and population structure. •
  
  
  RESULTS
  Eleven microsatellite markers were developed. The number of alleles per locus ranged from one to seven, and genetic diversity per locus ranged from 0.142 to 0.812. Four markers were successfully amplified in other species of sect. Brachycyrtis (T. ishiiana, T. ishiiana var. surugensis, and T. macranthopsis). •
  
  
  CONCLUSIONS
  The microsatellite markers can be used to investigate the genetic diversity and population structure of the vulnerable and endangered species of Tricyrtis sect. Brachycyrtis, to aid in the development of conservation strategies for each species.
authors.affiliation: Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Ohki
authors.affiliation: Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Setoguchi
keywords: Convallariaceae; Tricyrtis macrantha; cross-amplification; genetic diversity; microsatellite; population structure