title: Human umbilical cord mesenchymal stem cell-loaded amniotic membrane for the repair of radial nerve injury.
abstract: In this study, we loaded human umbilical cord mesenchymal stem cells onto human amniotic membrane with epithelial cells to prepare nerve conduits, i.e., a relatively closed nerve regeneration chamber. After neurolysis, the injured radial nerve was enwrapped with the prepared nerve conduit, which was fixed to the epineurium by sutures, with the cell on the inner surface of the conduit. Simultaneously, a 1.0 mL aliquot of human umbilical cord mesenchymal stem cell suspension was injected into the distal and proximal ends of the injured radial nerve with 1.0 cm intervals. A total of 1.75 × 10(7) cells were seeded on the amniotic membrane. In the control group, patients received only neurolysis. At 12 weeks after cell transplantation, more than 80% of patients exhibited obvious improvements in muscular strength, and touch and pain sensations. In contrast, these improvements were observed only in 55-65% of control patients. At 8 and 12 weeks, muscular electrophysiological function in the region dominated by the injured radial nerve was significantly better in the transplantation group than the control group. After cell transplantation, no immunological rejections were observed. These findings suggest that human umbilical cord mesenchymal stem cell-loaded amniotic membrane can be used for the repair of radial nerve injury.
authors.affiliation: Affiliated Central Hospital of Shenyang Medical College, Shenyang 110024, Liaoning Province, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Endocrinology and Metabolism, First Clinical Hospital of Norman Bethune College of Medicine, Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Hanjiao
authors.initials: H
authors.lastname: Qin
authors.affiliation: Shengjing Hospital, China Medical University, Shenyang 110000, Liaoning Province, China.
authors.forename: Zishan
authors.initials: Z
authors.lastname: Feng
authors.affiliation: Affiliated Central Hospital of Shenyang Medical College, Shenyang 110024, Liaoning Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Affiliated Central Hospital of Shenyang Medical College, Shenyang 110024, Liaoning Province, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Affiliated Central Hospital of Shenyang Medical College, Shenyang 110024, Liaoning Province, China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Yang
authors.affiliation: Affiliated Central Hospital of Shenyang Medical College, Shenyang 110024, Liaoning Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Human Anatomy and Histoembryology, Norman Bethune University of Medical Science, Changchun 130000, Jilin Province, China.
authors.forename: Youjun
authors.initials: Y
authors.lastname: Li
keywords: amniotic membrane; electrophysiology; human umbilical cord mesenchymal stem cells; motor; nerve conduit; nerve regeneration chamber; neural regeneration; neuroregeneration; peripheral nerve injury; radial nerve; sensory; stem cells