title: Phenotypic detection of carbapenemase-producing Enterobacteriaceae by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and the Carba NP test.
abstract: We compared the diagnostic accuracy of the Carba NP test with that of a straightforward matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) method for detecting carbapenemase-producing Enterobacteriaceae (CPE). Using PCR as the reference method, both tests demonstrated a sensitivity of 87% and a specificity of 100%. MALDI-TOF MS offers a potential alternative for the rapid detection of CPE in the clinical laboratory setting.
authors.affiliation: Microbiology Department, Melbourne Pathology, Melbourne, Australia jaknox2@yahoo.com.au.
authors.forename: James
authors.initials: J
authors.lastname: Knox
authors.affiliation: Department of Chemistry and Biotechnology, Swinburne University of Technology, Melbourne, Australia.
authors.forename: Snehal
authors.initials: S
authors.lastname: Jadhav
authors.affiliation: bioMérieux Australia Pty Ltd., Sydney, Australia.
authors.forename: Danielle
authors.initials: D
authors.lastname: Sevior
authors.affiliation: Westmead Millennium and Marie Bashir Institutes, University of Sydney, Sydney, Australia.
authors.forename: Alex
authors.initials: A
authors.lastname: Agyekum
authors.affiliation: Microbiological Diagnostic Unit, Public Health Laboratory, University of Melbourne, Melbourne, Australia.
authors.forename: Margaret
authors.initials: M
authors.lastname: Whipp
authors.affiliation: Microbiology Department, Melbourne Pathology, Melbourne, Australia.
authors.forename: Lynette
authors.initials: L
authors.lastname: Waring
authors.affiliation: Westmead Millennium and Marie Bashir Institutes, University of Sydney, Sydney, Australia Centre for Infectious Diseases and Microbiology, Westmead Hospital, Westmead, Australia.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Iredell
authors.affiliation: Department of Chemistry and Biotechnology, Swinburne University of Technology, Melbourne, Australia.
authors.forename: Enzo
authors.initials: E
authors.lastname: Palombo
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D001431:Bacteriological Techniques / Q000379:methods*; D004755:Enterobacteriaceae / Q000201:enzymology*; D006801:Humans; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase