title: Molecular characterization of the virulent microorganisms along with their drug-resistance traits associated with the export quality frozen shrimps in Bangladesh.
abstract: Current investigation characterized export quality shrimp samples in terms of pathogenic load along with the drug-resistance traits of the isolates, and detected the major virulent genes present in those isolates. Among the 30 such shrimp samples (15 each of Macrobrachium rosenbergi or Golda and Penaeus monodon or Bagda) studied, almost all were found to be contaminated with a huge load of bacteria (10(6)-10(8) cfu/g) and fungi (10(4)-10(5) cfu/g). Among the specific pathogens, presence of Escherichia coli, Vibrio spp., Aeromonas spp., Klebsiella spp., Shigella spp., Staphylococcus spp. and Listeria spp. were detected, of which most were likely to be resistant against commonly used antibiotics. Gene specific polymerase chain reaction (PCR) study revealed the presence of eae gene in E. coli, aero specific gene in Aeromonas spp., and sodB gene in Vibrio spp. Together with the huge extent of microbial contamination with a drug-resistance attribute, presence of such virulent genes further projects the probable public health risk upon consumption of the export quality shrimps.
authors.affiliation: Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka, 1217 Bangladesh.
authors.forename: Rashed
authors.initials: R
authors.lastname: Noor
authors.affiliation: Department of Microbiology, University of Dhaka, Fullar Road, Dhaka, 1000 Bangladesh.
authors.forename: Md Faqrul
authors.initials: MF
authors.lastname: Hasan
authors.affiliation: Department of Microbiology, University of Dhaka, Fullar Road, Dhaka, 1000 Bangladesh.
authors.forename: M Majibur
authors.initials: MM
authors.lastname: Rahman
keywords: Consumer health safety; Drug-resistance; Export quality frozen shrimps; Microorganisms; Virulence genes