title: Transcriptional Reprogramming of the Mycoparasitic Fungus Ampelomyces quisqualis During the Powdery Mildew Host-Induced Germination.
abstract: Ampelomyces quisqualis is a mycoparasite of a diverse range of phytopathogenic fungi associated with the powdery mildew disease. Among them are several Erysiphaceae species with great economic impact on high-value crops such as grape. Due to its ability to parasitize and prevent the spread of powdery mildews, A. quisqualis has received considerable attention for its biocontrol potential. However, and in sharp contrast to the extensively studied biocontrol species belonging to the genus Trichoderma, little is known about the biology of A. quisqualis at the molecular and genetic levels. We present the first genome-wide transcription profiling in A. quisqualis during host-induced germination. A total of 1,536 putative genes showed significant changes in transcription during the germination of A. quisqualis. This finding denotes an extensive transcriptional reprogramming of A. quisqualis induced by the presence of the host. Several upregulated genes were predicted to encode for putative mycoparasitism-related proteins such as secreted proteases, virulence factors, and proteins related to toxin biosynthesis. Our data provide the most comprehensive sequence resource currently available for A. quisqualis in addition to offering valuable insights into the biology of A. quisqualis and its mycoparasitic lifestyle. Eventually, this may improve the biocontrol capacity of this mycoparasite.
authors.forename: Stefanos
authors.initials: S
authors.lastname: Siozios
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Tosi
authors.forename: Alberto
authors.initials: A
authors.lastname: Ferrarini
authors.forename: Alessandro
authors.initials: A
authors.lastname: Ferrari
authors.forename: Paola
authors.initials: P
authors.lastname: Tononi
authors.forename: Diana
authors.initials: D
authors.lastname: Bellin
authors.forename: Monika
authors.initials: M
authors.lastname: Maurhofer
authors.forename: Cesare
authors.initials: C
authors.lastname: Gessler
authors.forename: Massimo
authors.initials: M
authors.lastname: Delledonne
authors.forename: Ilaria
authors.initials: I
authors.lastname: Pertot
mesh_terms: D001203:Ascomycota / Q000235:genetics* / Q000502:physiology; D061046:Biological Control Agents; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control*; D012331:RNA, Fungal / Q000235:genetics; D059467:Transcriptome; D027843:Vitis / Q000382:microbiology*
chemical_list: D061046:Biological Control Agents; D018076:DNA, Complementary; D012331:RNA, Fungal
keywords: gene expression profiling; transcriptome