title: Biopsy of human morula-stage embryos: outcome of 215 IVF/ICSI cycles with PGS.
abstract: Preimplantation genetic diagnosis (PGD) is commonly performed on biopsies from 6-8-cell-stage embryos or blastocyst trophectoderm obtained on day 3 or 5, respectively. Day 4 human embryos at the morula stage were successfully biopsied. Biopsy was performed on 709 morulae from 215 ICSI cycles with preimplantation genetic screening (PGS), and 3-7 cells were obtained from each embryo. The most common vital aneuploidies (chromosomes X/Y, 21) were screened by fluorescence in situ hybridization (FISH). No aneuploidy was observed in 72.7% of embryos, 91% of those developed to blastocysts. Embryos were transferred on days 5-6. Clinical pregnancy was obtained in 32.8% of cases, and 60 babies were born. Patients who underwent ICSI/PGS treatment were compared with those who underwent standard ICSI treatment by examining the percentage of blastocysts, pregnancy rate, gestational length, birth height and weight. No significant differences in these parameters were observed between the groups. Day 4 biopsy procedure does not adversely affect embryo development in vitro or in vivo. The increased number of cells obtained by biopsy of morulae might facilitate diagnostic screening. There is enough time after biopsy to obtain PGD results for embryo transfer on day 5-6 in the current IVF cycle.
authors.affiliation: Center for Reproductive Medicine MAMA, Moscow, Russian Federation.
authors.forename: Elena E
authors.initials: EE
authors.lastname: Zakharova
authors.affiliation: Center for Reproductive Medicine MAMA, Moscow, Russian Federation.
authors.forename: Victoria V
authors.initials: VV
authors.lastname: Zaletova
authors.affiliation: Center for Reproductive Medicine MAMA, Moscow, Russian Federation.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Krivokharchenko
mesh_terms: D000328:Adult; D001706:Biopsy; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009028:Morula / Q000166:cytology*; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D019836:Preimplantation Diagnosis; D020554:Sperm Injections, Intracytoplasmic