title: Kisspeptin regulates gonadotropin-releasing hormone secretion in gonadotropin-releasing hormone/enhanced green fluorescent protein transgenic rats.
abstract: Kisspeptin is essential for activation of the hypothalamo-pituitary-gonadal axis. In this study, we established gonadotropin-releasing hormone/enhanced green fluorescent protein transgenic rats. Rats were injected with 1, 10, or 100 pM kisspeptin-10, a peptide derived from full-length kisspeptin, into the arcuate nucleus and medial preoptic area, and with the kisspeptin antagonist peptide 234 into the lateral cerebral ventricle. The results of immunohistochemical staining revealed that pulsatile luteinizing hormone secretion was suppressed after injection of antagonist peptide 234 into the lateral cerebral ventricle, and a significant increase in luteinizing hormone level was observed after kisspeptin-10 injection into the arcuate nucleus and medial preoptic area. The results of an enzyme-linked immunosorbent assay showed that luteinizing hormone levels during the first hour of kisspeptin-10 infusion into the arcuate nucleus were significantly greater in the 100 pM kisspeptin-10 group than in the 10 pM kisspeptin-10 group. These findings indicate that kisspeptin directly promotes gonadotropin-releasing hormone secretion and luteinizing hormone release in gonadotropin-releasing hormone/enhanced green fluorescent protein transgenic rats. The arcuate nucleus is a key component of the kisspeptin-G protein-coupled receptor 54 signaling pathway underlying regulating luteinizing hormone pulse secretion.
authors.affiliation: Department of Orthopedic Surgery, First Clinical Hospital, Beihua University, Jilin 132011, Jilin Province, China.
authors.forename: Haogang
authors.initials: H
authors.lastname: Xue
authors.affiliation: Department of Orthopedic Surgery, First Clinical Hospital, Beihua University, Jilin 132011, Jilin Province, China.
authors.forename: Chunying
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Orthopedic Surgery, First Clinical Hospital, Beihua University, Jilin 132011, Jilin Province, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Ge
authors.affiliation: Department of Orthopedic Surgery, First Clinical Hospital, Beihua University, Jilin 132011, Jilin Province, China.
authors.forename: Weiqi
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Orthopedic Surgery, First Clinical Hospital, Beihua University, Jilin 132011, Jilin Province, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Orthopedic Surgery, First Clinical Hospital, Beihua University, Jilin 132011, Jilin Province, China.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Qi
keywords: G protein-coupled receptor 54; anteroventral periventricular nucleus; arcuate nucleus; basic research; enhanced green fluorescent protein; gonadotropin-releasing hormone; luteinizing hormone; medial preoptic area; neural regeneration; neuroregeneration; organum vasculosum of the lamina terminalis; photographs-containing paper; transgenic