title: Comparative analysis of caveolins in mouse and tammar wallaby: role in regulating mammary gland function.
abstract: Recent studies using the mouse showed an inverse correlation between the Caveolin 1 gene expression and lactation, and this was regulated by prolactin. However, current study using mammary explants from pregnant mice showed that while insulin (I), cortisol (F) and prolactin (P) resulted in maximum induction of the β-casein gene, FP and IFP resulted in the downregulation of Caveolin 1. Additionally, IF, FP and IFP resulted in the downregulation of Caveolin 2. Immunohistochemistry confirmed localisation of Caveolin 1 specific to myoepithelial cells and adipocytes. Comparative studies with the tammar wallaby showed Caveolin 1 and 2 had 70-80% homology with the mouse proteins. However, in contrast to the mouse, Caveolin 1 and 2 genes showed a significantly increased level of expression in the mammary gland during lactation. The regulation of tammar Caveolin 1 and 2 gene expression was examined in mammary explants from pregnant tammars, and no significant difference was observed either in the absence or in the presence of IFP.
authors.affiliation: School of Medicine, Deakin University, Pigdons Rd., 3217 VIC, Australia. Electronic address: sanjanakurupath@gmail.com.
authors.forename: Sanjana
authors.initials: S
authors.lastname: Kuruppath
authors.affiliation: School of Medicine, Deakin University, Pigdons Rd., 3217 VIC, Australia.
authors.forename: Julie A
authors.initials: JA
authors.lastname: Sharp
authors.affiliation: School of Medicine, Deakin University, Pigdons Rd., 3217 VIC, Australia.
authors.forename: Christophe
authors.initials: C
authors.lastname: Lefevre
authors.affiliation: Department of Zoology, La Trobe University, Melbourne, 3086 VIC, Australia.
authors.forename: Robyn M
authors.initials: RM
authors.lastname: Murphy
authors.affiliation: School of Medicine, Deakin University, Pigdons Rd., 3217 VIC, Australia; Department of Anatomy and Developmental Biology, Monash University, Clayton, 3800 VIC, Australia.
authors.forename: Kevin R
authors.initials: KR
authors.lastname: Nicholas
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002364:Caseins / Q000235:genetics; D051242:Caveolin 1 / Q000235:genetics*; D051243:Caveolin 2 / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D015870:Gene Expression / Q000235:genetics; D006728:Hormones / Q000235:genetics; D006854:Hydrocortisone / Q000235:genetics; D007328:Insulin / Q000235:genetics; D007774:Lactation / Q000235:genetics; D007614:Macropodidae / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011247:Pregnancy; D011388:Prolactin / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D002364:Caseins; D051242:Caveolin 1; D051243:Caveolin 2; D006728:Hormones; D007328:Insulin; D011388:Prolactin; D006854:Hydrocortisone
keywords: Caveolin; Hormones; Lactation; Mammary gland; Mouse; Tammar wallaby