title: In vitro selection of a single-stranded DNA molecular recognition element against atrazine.
abstract: Widespread use of the chlorotriazine herbicide, atrazine, has led to serious environmental and human health consequences. Current methods of detecting atrazine contamination are neither rapid nor cost-effective. In this work, atrazine-specific single-stranded DNA (ssDNA) molecular recognition elements (MRE) were isolated. We utilized a stringent Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methodology that placed the greatest emphasis on what the MRE should not bind to. After twelve rounds of SELEX, an atrazine-specific MRE with high affinity was obtained. The equilibrium dissociation constant (Kd) of the ssDNA sequence is 0.62 ± 0.21 nM. It also has significant selectivity for atrazine over atrazine metabolites and other pesticides found in environmentally similar locations and concentrations. Furthermore, we have detected environmentally relevant atrazine concentrations in river water using this MRE. The strong affinity and selectivity of the selected atrazine-specific ssDNA validated the stringent SELEX methodology and identified a MRE that will be useful for rapid atrazine detection in environmental samples.
authors.affiliation: Department of Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, PO Box 9530, Morgantown, WV 26506, USA. rwilliams@mix.wvu.edu.
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Williams
authors.affiliation: Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV 26506, USA. clcrihfield@mix.wvu.edu.
authors.forename: Cassandra L
authors.initials: CL
authors.lastname: Crihfield
authors.affiliation: Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV 26506, USA. srgattu@mix.wvu.edu.
authors.forename: Srikanth
authors.initials: S
authors.lastname: Gattu
authors.affiliation: Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV 26506, USA. lisa.holland@mail.wvu.edu.
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Holland
authors.affiliation: Department of Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, PO Box 9530, Morgantown, WV 26506, USA. lsooter@hsc.wvu.edu.
authors.forename: Letha J
authors.initials: LJ
authors.lastname: Sooter
mesh_terms: D001280:Atrazine / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D066298:In Vitro Techniques
chemical_list: D004277:DNA, Single-Stranded; D001280:Atrazine