title: Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone.
abstract: Several hundred monokaryotic and new dikaryotic strains derived thereof were established from (+)-valencene tolerant Pleurotus species. When grouped according to their growth rate on agar plates and compared to the parental of Pleurotus sapidus 69, the slowly growing monokaryons converted (+)-valencene more efficiently to the grapefruit flavour compound (+)-nootkatone. The fast growing monokaryons and the slow×slow and the fast×fast dikaryotic crosses showed similar or inferior yields. Some slow×fast dikaryons, however, exceeded the biotransformation capability of the parental dikaryon significantly. The activity of the responsible enzyme, lipoxygenase, showed a weak correlation with the yields of (+)-nootkatone indicating that the determination of enzyme activity using the primary substrate linoleic acid may be misleading in predicting the biotransformation efficiency. This exploratory study indicated that a classical genetics approach resulted in altered and partly improved terpene transformation capability (plus 60%) and lipoxygenase activity of the strains.
authors.affiliation: Institut für Lebensmittelchemie, Leibniz Universität Hannover, Callinstraße 5, 30167 Hannover, Germany. Electronic address: alejandra.omarini@lci.uni-hannover.de.
authors.forename: Alejandra B
authors.initials: AB
authors.lastname: Omarini
authors.affiliation: Institut für Lebensmittelchemie, Leibniz Universität Hannover, Callinstraße 5, 30167 Hannover, Germany.
authors.forename: Ina
authors.initials: I
authors.lastname: Plagemann
authors.affiliation: Institut für Lebensmittelchemie, Leibniz Universität Hannover, Callinstraße 5, 30167 Hannover, Germany.
authors.forename: Silke
authors.initials: S
authors.lastname: Schimanski
authors.affiliation: Institut für Lebensmittelchemie, Leibniz Universität Hannover, Callinstraße 5, 30167 Hannover, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Krings
authors.affiliation: Institut für Lebensmittelchemie, Leibniz Universität Hannover, Callinstraße 5, 30167 Hannover, Germany.
authors.forename: Ralf G
authors.initials: RG
authors.lastname: Berger
mesh_terms: D001709:Biotechnology / Q000379:methods; D001711:Biotransformation / Q000502:physiology*; D003433:Crosses, Genetic; D049770:Genes, Mating Type, Fungal / Q000502:physiology; D019787:Linoleic Acid; D008084:Lipoxygenase / Q000378:metabolism; D020076:Pleurotus / Q000235:genetics* / Q000502:physiology*; D000081223:Polycyclic Sesquiterpenes; D011136:Polysorbates; D012717:Sesquiterpenes / Q000378:metabolism*; D013045:Species Specificity; D018709:Statistics, Nonparametric
chemical_list: D000081223:Polycyclic Sesquiterpenes; D011136:Polysorbates; D012717:Sesquiterpenes; C506706:valencene; D019787:Linoleic Acid; D008084:Lipoxygenase; C050302:nootkatone
keywords: (+)-Valencene; Basidiomycota; Biotransformation; Monokaryon; Pleurotus