title: [Umbilical cord blood-derived mesenchymal stem cells inhibit proliferation of peripheral blood lymphocytes].
abstract: OBJECTIVE
  To study the effect of umbilical cord blood-derived mesenchymal stem cells (UCBMSCs) on the proliferation of adult peripheral blood lymphocytes.
  
  
  METHODS
  MSCs were isolated and amplified from human UCB and identified by detecting the surface markers and the ability of differentiation into osteoblasts and adipocytes. Lymphocytes were stimulated with phytohemagglutinin (PHA) and co-cultured with UCBMSCs, or cultured with UCBMSCs in non-contact Transwell(TM) system. Two culture systems were both divided into negative control group (lymphocytes were cultured alone), positive control group (lymphocytes were stimulated with PHA), and experimental group (lymphocytes were cultured with UCBMSCs and stimulated with PHA). The proliferation of lymphocytes was examined by MTS assay, and the inhibition rate was calculated.
  
  
  RESULTS
  In both culture systems, UCBMSCs could inhibit the proliferation of peripheral blood lymphocytes in a dose-dependent manner (P<0.05). But when the proportion of UCBMSCs to lymphocytes of the two systems was the same, the inhibitory effect of co-culture system was significantly stronger than that of Transwell(TM) system (P<0.05).
  
  
  CONCLUSIONS
  UCBMSCs can inhibit the proliferation of peripheral blood lymphocytes, and the inhibitory effect of co-culture system is stronger than that of Transwell(TM) system.
authors.affiliation: First Affiliated Hospital, School of Medicine, Ningbo University, Ningbo 315000, China.
authors.forename: Zhijuan
authors.initials: Z
authors.lastname: Xu
authors.affiliation: First Affiliated Hospital, School of Medicine, Ningbo University, Ningbo 315000, China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Sheng
authors.affiliation: First Affiliated Hospital, School of Medicine, Ningbo University, Ningbo 315000, China.
authors.forename: Guifang
authors.initials: G
authors.lastname: Ouyang
mesh_terms: D000328:Adult; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002454:Cell Differentiation / Q000276:immunology; D049109:Cell Proliferation; D002470:Cell Survival / Q000276:immunology; D002478:Cells, Cultured; D018920:Coculture Techniques; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology*; D010835:Phytohemagglutinins / Q000276:immunology / Q000494:pharmacology
chemical_list: D010835:Phytohemagglutinins