title: Bioluminescent probe for hydrogen peroxide imaging in vitro and in vivo.
abstract: Reactive oxygen species (ROS) often have significant roles in mediating redox modifications and other essential physiological processes, such as biological process regulation and signal transduction. Considering that H2O2 is a substantial member of ROS, detection and quantitation of H2O2 undertakes important but urgent responsibility. In this report, a bioluminescent probe for detecting H2O2 was well designed, synthesized, and evaluated. This probe was designed into three parts: a H2O2-sensitive aryl boronic acid, a bioluminescent aminoluciferin moiety, and a self-immolative linker. After extensive evaluation, this probe can selectively and sensitively react with H2O2 to release aminoluciferin. It should be pointed out that this probe is a potential bioluminescent sensor for H2O2 since it can provide a promising toolkit for real-time detection of the H2O2 level in vitro, in cellulo, and in vivo.
authors.affiliation: Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Shandong University , Jinan, Shandong 250012, China.
authors.forename: Wenxiao
authors.initials: W
authors.lastname: Wu
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Laizhong
authors.initials: L
authors.lastname: Chen
authors.forename: Zhao
authors.initials: Z
authors.lastname: Ma
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Yanna
authors.initials: Y
authors.lastname: Cheng
authors.forename: Lupei
authors.initials: L
authors.lastname: Du
authors.forename: Minyong
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D001897:Boronic Acids / Q000737:chemistry*; D045744:Cell Line, Tumor; D005260:Female; D049454:Firefly Luciferin / Q000031:analogs & derivatives*; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements; D008297:Male; D051379:Mice; D008819:Mice, Nude; D057054:Molecular Imaging / Q000379:methods*; D015335:Molecular Probes / Q000737:chemistry*; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D001897:Boronic Acids; D049408:Luminescent Agents; D015335:Molecular Probes; D017382:Reactive Oxygen Species; D049454:Firefly Luciferin; D006861:Hydrogen Peroxide