title: Dew inspired breathing-based detection of genetic point mutation visualized by naked eye.
abstract: A novel label-free method based on breathing-induced vapor condensation was developed for detection of genetic point mutation. The dew-inspired detection was realized by integration of target-induced DNA ligation with rolling circle amplification (RCA). The vapor condensation induced by breathing transduced the RCA-amplified variances in DNA contents into visible contrast. The image could be recorded by a cell phone for further or even remote analysis. This green assay offers a naked-eye-reading method potentially applied for point-of-care liver cancer diagnosis in resource-limited regions.
authors.affiliation: 1] Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China [2] Institute of Molecular Medicine, College of Life and Health Science, Northeastern University, Shenyang 110004, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Xie
authors.affiliation: Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Tongzhou
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Tianqi
authors.initials: T
authors.lastname: Huang
authors.affiliation: Tianjin Second People's Hospital and Tianjin Institute of Hepatology, Tianjin 300192, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hou
authors.affiliation: Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Huang
authors.affiliation: 1] Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China [2] Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Du
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000235:genetics; D055088:Early Detection of Cancer / Q000379:methods; D005898:Glass; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D017354:Point Mutation / Q000235:genetics*; D012119:Respiration; D013194:Staining and Labeling / Q000379:methods*; D013227:Steam; D013499:Surface Properties; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D017155:Wettability
chemical_list: D013227:Steam; D016159:Tumor Suppressor Protein p53