title: Versatile matrix for constructing enzyme-based biosensors.
abstract: A versatile matrix was fabricated and utilized as a universal interface for the construction of enzyme-based biosensors. This matrix was formed on the gold electrode via combining self-assembled monolayer of 2,3-dimercaptosuccinic acid with gold nanoparticles. Gold nanoparticles were electrochemically deposited. Electrochemistry of three redox enzymes (catalase, glucose oxidase, and horseradish peroxidase) was investigated on such a matrix. The electrocatalytic monitoring of hydrogen peroxide and glucose was conducted on this matrix after being coated with those enzymes. On them the monitoring of hydrogen peroxide and glucose shows rapid response times, wide linear working ranges, low detection limits, and high enzymatic affinities. This matrix is thus a versatile and suitable platform to develop highly sensitive enzyme-based biosensors.
authors.affiliation: School of Chemistry and Environmental Engineering, Wuhan Institute of Technology , Wuhan 430073, China.
authors.forename: Zhaohao
authors.initials: Z
authors.lastname: Wang
authors.forename: Xi
authors.initials: X
authors.lastname: Luo
authors.forename: Qijin
authors.initials: Q
authors.lastname: Wan
authors.forename: Kangbing
authors.initials: K
authors.lastname: Wu
authors.forename: Nianjun
authors.initials: N
authors.lastname: Yang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002374:Catalase / Q000378:metabolism*; D055664:Electrochemical Techniques; D004566:Electrodes; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase / Q000378:metabolism*; D006046:Gold / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force; D004113:Succimer / Q000737:chemistry
chemical_list: D006046:Gold; D006861:Hydrogen Peroxide; D004113:Succimer; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D002374:Catalase; D005947:Glucose
keywords: gold nanoparticles; hydrogen peroxide biosensors; redox enzyme; self-assembled monolayers; versatile matrix