title: Comparison of proteases in DNA extraction via quantitative polymerase chain reaction.
abstract: We compared four proteases in the QIAamp DNA Investigator Kit (Qiagen) to extract DNA for use in multiplex polymerase chain reaction (PCR) assays. The aim was to evaluate alternate proteases for improved DNA recovery as compared with proteinase K for forensic, biochemical research, genetic paternity and immigration, and molecular diagnostic purposes. The Quantifiler Kit TaqMan quantitative PCR assay was used to measure the recovery of DNA from human blood, semen, buccal cells, breastmilk, and earwax in addition to low-template samples, including diluted samples, computer keyboard swabs, chewing gum, and cigarette butts. All methods yielded amplifiable DNA from all samples.
authors.affiliation: Department of Chemistry, Towson University, Towson, MD 21252, USA.
authors.forename: Alison M
authors.initials: AM
authors.lastname: Eychner
authors.affiliation: Department of Chemistry, Towson University, Towson, MD 21252, USA.
authors.forename: Roberta J
authors.initials: RJ
authors.lastname: Lebo
authors.affiliation: Department of Chemistry, Towson University, Towson, MD 21252, USA. Electronic address: kmelkins@towson.edu.
authors.forename: Kelly M
authors.initials: KM
authors.lastname: Elkins
mesh_terms: D031230:Ananas / Q000201:enzymology; D001963:Bromelains / Q000378:metabolism; D029441:Carica / Q000201:enzymology; D004247:DNA / Q000032:analysis / Q000097:blood / Q000302:isolation & purification* / Q000378:metabolism*; D019286:Endopeptidase K / Q000378:metabolism; D005658:Fungi / Q000201:enzymology; D006801:Humans; D010206:Papain / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic
chemical_list: D011933:Reagent Kits, Diagnostic; D001963:Bromelains; D004247:DNA; D010447:Peptide Hydrolases; D019286:Endopeptidase K; D010206:Papain
keywords: Bromelain; DNA extraction; Papain; Proteinase K; QIAamp; Quantitative real-time PCR