title: Engineering a sensitive visual-tracking reporter system for   real-time monitoring phosphorus deficiency in tobacco.
abstract: Plant phosphorus (P) diagnosis is widely used for monitoring P status and guiding P fertilizer application in field conditions. The common methods for predicting plant response to P are time- and labour-consuming chemical measurements of the extractable soil P and plant P concentrations. In this study, we successfully generated a visual reporter system in tobacco (Nicotiana tabacum L.) to monitor plant P status by expressing of a Purple gene (Pr) isolated from cauliflower (Brassica oleracea var botrytis) driven by the promoter (Pro) of OsPT6, a P-starvation-induced rice gene. The leaves of OsPT6pro::Pr (PT6pro::Pr) transgenic tobacco continuously turned into dark purple with the increase of duration and severity of P deficiency, and recovered rapidly to basal green colour upon resupply of P. The expression of several anthocyanin biosynthesis involving genes was strongly activated in the transgenic tobacco in comparison to wild type under P-deficient condition. Such additive purple colour was not detected by deficiencies of other major- and micronutrients or stresses of salt, drought and cold. There was an extremely high correlation between P concentration and anthocyanin accumulation in the transgenic tobacco leaves. Using a hyperspectral sensing technology, P concentration in the leaves of transgenic plants could be predicted by the reflectance spectra at 554 nm wavelength with approximately 0.16 as the threshold value of the P deficiency. Taken together, the colour-based visual reporter system could be specifically and readily used for monitoring the plant P status by naked eyes and accurately assessed by spectral reflectance.
authors.forename: Yiting
authors.initials: Y
authors.lastname: Li
authors.forename: Mian
authors.initials: M
authors.lastname: Gu
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Hongmei
authors.initials: H
authors.lastname: Fan
authors.forename: Panpan
authors.initials: P
authors.lastname: Li
authors.forename: Zhaofu
authors.initials: Z
authors.lastname: Li
authors.forename: Guohua
authors.initials: G
authors.lastname: Xu
mesh_terms: D000872:Anthocyanins / Q000096:biosynthesis; D053898:Biosynthetic Pathways / Q000187:drug effects / Q000235:genetics; D003199:Computer Systems; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods*; D005966:Glucuronidase / Q000378:metabolism; D012275:Oryza / Q000187:drug effects / Q000235:genetics; D010758:Phosphorus / Q000172:deficiency* / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics*
chemical_list: D000872:Anthocyanins; D012333:RNA, Messenger; D010758:Phosphorus; D005966:Glucuronidase