title: Whole genome and transcriptome analyses of environmental antibiotic sensitive and multi-resistant Pseudomonas aeruginosa isolates exposed to waste water and tap water.
abstract: The fitness of sensitive and resistant Pseudomonas aeruginosa in different aquatic environments depends on genetic capacities and transcriptional regulation. Therefore, an antibiotic-sensitive isolate PA30 and a multi-resistant isolate PA49 originating from waste waters were compared via whole genome and transcriptome Illumina sequencing after exposure to municipal waste water and tap water. A number of different genomic islands (e.g. PAGIs, PAPIs) were identified in the two environmental isolates beside the highly conserved core genome. Exposure to tap water and waste water exhibited similar transcriptional impacts on several gene clusters (antibiotic and metal resistance, genetic mobile elements, efflux pumps) in both environmental P. aeruginosa isolates. The MexCD-OprJ efflux pump was overexpressed in PA49 in response to waste water. The expression of resistance genes, genetic mobile elements in PA49 was independent from the water matrix. Consistently, the antibiotic sensitive strain PA30 did not show any difference in expression of the intrinsic resistance determinants and genetic mobile elements. Thus, the exposure of both isolates to polluted waste water and oligotrophic tap water resulted in similar expression profiles of mentioned genes. However, changes in environmental milieus resulted in rather unspecific transcriptional responses than selected and stimuli-specific gene regulation.
authors.affiliation: Institute of Functional Interfaces (IFG), Campus North, Karlsruhe Institute of Technology (KIT), Hermann von Helmholtz Platz 1, Eggenstein-Leopoldshafen, D-76344, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Schwartz
authors.forename: Olivier
authors.initials: O
authors.lastname: Armant
authors.forename: Nancy
authors.initials: N
authors.lastname: Bretschneider
authors.forename: Alexander
authors.initials: A
authors.lastname: Hahn
authors.forename: Silke
authors.initials: S
authors.lastname: Kirchen
authors.forename: Martin
authors.initials: M
authors.lastname: Seifert
authors.forename: Andreas
authors.initials: A
authors.lastname: Dötsch
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D004783:Environmental Microbiology; D005618:Fresh Water / Q000382:microbiology*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D062065:Wastewater / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D062065:Wastewater