title: Changes of serum parameters of TiO₂ nanoparticle-induced atherosclerosis in mice.
abstract: The evaluation of toxicological effects of nanoparticulate matter is increasingly important due to their growing occupational use and presence as compounds in consumer products. Numerous studies have shown that exposure to nanosized particles lead to systemic inflammation in experimental animals, but whether long-term exposure to nanosized particles induces atherogenesis is rarely evaluated. In the current study, mice were continuously exposed to TiO2 nanoparticles (NPs) at 1.25, 2.5, or 5mg/kg body weight, administered by nasal instillation for nine consecutive months, and the association between serum parameter changes and atherosclerosis in mice were investigated. The present findings suggested that chronic exposure to TiO2 NPs resulted in atherogenesis coupling with pulmonary inflammation, increased levels of serum triglycerides, glucose, total cholesterol, low-density lipoprotein cholesterol, advanced glycation end products, reactive oxygen species, NAD(P)H oxidases 4, C-reaction protein, E-selectin, endothelin-1, tissue factor, intercellular adhesion molecule-1, vascular cell adhesion molecule-1, monocyte chemoattractant protein-1, plasminogen activator inhibitor-1, and reduced levels of serum high-density lipoprotein cholesterol, nitric oxide and tissue plasminogen activator. Our study suggests an association of long-term exposure to TiO2 NPs with atherosclerosis and pulmonary inflammation. This finding demonstrates the hypothesized role of TiO2 NPs as a risk factor for atherogenesis.
authors.affiliation: Medical College of Soochow University, Suzhou 215123, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Yu
authors.affiliation: Medical College of Soochow University, Suzhou 215123, China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Medical College of Soochow University, Suzhou 215123, China.
authors.forename: Yuguan
authors.initials: Y
authors.lastname: Ze
authors.affiliation: Library of Soochow University, Suzhou 215123, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: Medical College of Soochow University, Suzhou 215123, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.affiliation: Medical College of Soochow University, Suzhou 215123, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Hong
authors.affiliation: Medical College of Soochow University, Suzhou 215123, China.
authors.forename: Bingqing
authors.initials: B
authors.lastname: Xu
authors.affiliation: Medical College of Soochow University, Suzhou 215123, China.
authors.forename: Anan
authors.initials: A
authors.lastname: Lin
authors.affiliation: Medical College of Soochow University, Suzhou 215123, China.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Medical College of Soochow University, Suzhou 215123, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Medical College of Soochow University, Suzhou 215123, China. Electronic address: libingyan@suda.edu.cn.
authors.forename: Bingyan
authors.initials: B
authors.lastname: Li
authors.affiliation: Medical College of Soochow University, Suzhou 215123, China. Electronic address: hongfsh_cn@sina.com.
authors.forename: Fashui
authors.initials: F
authors.lastname: Hong
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000097:blood / Q000139:chemically induced*; D015415:Biomarkers / Q000097:blood*; D005260:Female; D008055:Lipids / Q000097:blood; D008168:Lung / Q000187:drug effects / Q000276:immunology; D008813:Mice, Inbred ICR; D053758:Nanoparticles / Q000633:toxicity*; D011897:Random Allocation; D014025:Titanium / Q000097:blood / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D008055:Lipids; C009495:titanium dioxide; D014025:Titanium
keywords: Atherosclerosis; Mice; Pulmonary inflammation; Serum parameters; Titanium dioxide nanoparticles