title: An evaluation of background levels and sources of polycyclic aromatic hydrocarbons in naturally spawned embryos of Pacific herring (Clupea pallasii) from Puget Sound, Washington, USA.
abstract: Pacific herring embryos spawned in nearshore habitats may be exposed to toxic contaminants as they develop, from exogenous sources in spawning habitats and from maternal transfer. Determining baseline concentrations of these toxic contaminants is important for evaluating the health of this species, especially during this sensitive life stage. In this study we compared concentrations of polycyclic aromatic hydrocarbons, or PAHs, in naturally spawned herring embryos from five spawning areas across Puget Sound. The summed values of 31 PAH analytes (Σ31PAH) in early- to late-stage development embryos ranged from 1.1 to 140 ng/g, wet weight. Σ31PAH concentrations increased with development time in embryos from one spawning area where the greatest concentrations were observed, and the relative abundance of PAH chemicals in late-stage embryos was similar to those in nearby sediments, suggesting accumulation from local environmental sources. PAHs in both sediments and late-stage embryos appeared to exhibit a pyrogenic pattern. Although maternal transfer of PAHs appeared to be a negligible source to embryos in spawning areas with the greatest embryo PAH concentrations, maternal transfer may have been the dominant source in embryos from spawning areas where the lowest levels of embryo-PAHs occurred. Chronic embryo mortality has been reported in spawning habitats where we observed the greatest concentration of PAHs in embryos, and necrotic tissue in herring embryos from one such location was similar in description to phototoxic PAH necrosis reported elsewhere for embryonic zebrafish.
authors.affiliation: Washington Department of Fish and Wildlife, Fish Program/Marine Resources Division, 600 Capitol Way N, Olympia, WA 98501-1091, USA. Electronic address: james.west@dfw.wa.gov.
authors.forename: James E
authors.initials: JE
authors.lastname: West
authors.affiliation: Washington Department of Fish and Wildlife, Fish Program/Marine Resources Division, 600 Capitol Way N, Olympia, WA 98501-1091, USA. Electronic address: sandra.oneill@dfw.wa.gov.
authors.forename: Sandra M
authors.initials: SM
authors.lastname: O'Neill
authors.affiliation: NOAA Fisheries, Northwest Fisheries Science Center, 2725 Montlake Boulevard East, Seattle, WA 98112, USA. Electronic address: gina.ylitalo@noaa.gov.
authors.forename: Gina M
authors.initials: GM
authors.lastname: Ylitalo
authors.affiliation: NOAA Fisheries, Northwest Fisheries Science Center, 2725 Montlake Boulevard East, Seattle, WA 98112, USA. Electronic address: john.incardona@noaa.gov.
authors.forename: John P
authors.initials: JP
authors.lastname: Incardona
authors.affiliation: Washington Department of Fish and Wildlife, Fish Program/Marine Resources Division, 600 Capitol Way N, Olympia, WA 98501-1091, USA. Electronic address: dan.doty@dfw.wa.gov.
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Doty
authors.affiliation: Washington Department of Ecology, Marine Monitoring Unit, 300 Desmond Drive, PO Box 47600, Olympia, WA 98504-7600, USA. Electronic address: margaret.dutch@ecy.wa.gov.
authors.forename: Margaret E
authors.initials: ME
authors.lastname: Dutch
mesh_terms: D000818:Animals; D017753:Ecosystem; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D004784:Environmental Monitoring; D005399:Fishes / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism* / Q000592:standards; D014861:Washington; D014874:Water Pollutants, Chemical / Q000378:metabolism* / Q000592:standards
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical
keywords: Contaminants; Embryos; Herring; Maternal transfer; PAHs; Puget Sound