title: [Effect of temperature on prodigiosin synthesis in Serratia marcecens].
abstract: OBJECTIVE
  To study the effect of temperature on prodigiosin synthesis in Serratia marcecens JNB5-1.
  
  
  METHODS
  Different proteins obtained after culturing S. marcecens JNB5-1 at 28 degrees C and 37 degrees C were separated and identified through two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry. Subsequently, 7 of the proteins were further analyzed by real-time qPCR.
  
  
  RESULTS
  We identified 16 different proteins. Among them, prodigiosin biosynthetic enzymes (O-methyl transferase and Oxidoreductase) and proteins related with the precursor substances (proine, methionine, serine, 2-Octenal and Malonyl-CoA) of the prodigiosin biosynthetic were significantly down-expressed at 37 degrees C. Heat shock protein (Hsp60) and superoxide dismutase were up-expressed at 37degrees C. The results of real-time qPCR show that the mRNA transcriptional levels of o-methyl transferase, oxidoreductase and transketolase at 37 degrees C were all lower than that at 28 degrees C.
  
  
  CONCLUSIONS
  S. marcecens JNB5-1 could produce prodigiosin at lower temperature (28 degrees C) but not at higher temperature (37 degrees C). Higher temperature probably inhibited the expression of enzymes related with prodigiosin biosynthesis.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.forename: Meijuan
authors.initials: M
authors.lastname: Xu
authors.forename: Taowei
authors.initials: T
authors.lastname: Yang
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Rao
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D053898:Biosynthetic Pathways; D015180:Electrophoresis, Gel, Two-Dimensional; D008969:Molecular Sequence Data; D011353:Prodigiosin / Q000096:biosynthesis*; D012705:Serratia / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D011353:Prodigiosin