title: Genome sequence and emended description of Leisingera nanhaiensis strain DSM 24252(T) isolated from marine sediment.
abstract: Leisingera nanhaiensis DSM 24252(T) is a Gram-negative, motile, rod-shaped marine Alphaproteobacterium, isolated from sandy marine sediments. Here we present the non-contiguous genome sequence and annotation together with a summary of the organism's phenotypic features. The 4,948,550 bp long genome with its 4,832 protein-coding and 64 RNA genes consists of one chromosome and six extrachromosomal elements with lengths of 236 kb, 92 kb, 61 kb, 58 kb, 56 kb, and 35 kb, respectively. The analysis of the genome showed that DSM 24252(T) possesses all genes necessary for dissimilatory nitrite reduction, and the strain was shown to be facultatively anaerobic, a deviation from the original description that calls for an emendation of the species. Also present in the genome are genes coding for a putative prophage, for gene-transfer agents and for the utilization of methylated amines. Phylogenetic analysis and intergenomic distances indicate that L. nanhaiensis might not belong to the genus Leisingera.
authors.affiliation: Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, Oldenburg, Germany .
authors.forename: Sven
authors.initials: S
authors.lastname: Breider
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors.forename: Hazuki
authors.initials: H
authors.lastname: Teshima
authors.affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
authors.forename: Jörn
authors.initials: J
authors.lastname: Petersen
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors.forename: Olga
authors.initials: O
authors.lastname: Chertkov
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors.forename: Hajnalka
authors.initials: H
authors.lastname: Dalingault
authors.affiliation: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Chen
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Amrita
authors.initials: A
authors.lastname: Pati
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Ivanova
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Alla
authors.initials: A
authors.lastname: Lapidus
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA ; DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Lynne A
authors.initials: LA
authors.lastname: Goodwin
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors.forename: Patrick
authors.initials: P
authors.lastname: Chain
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA ; DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: John C
authors.initials: JC
authors.lastname: Detter
authors.affiliation: HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Rohde
authors.affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Tindall
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Nikos C
authors.initials: NC
authors.lastname: Kyrpides
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Tanja
authors.initials: T
authors.lastname: Woyke
authors.affiliation: Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, Oldenburg, Germany .
authors.forename: Meinhard
authors.initials: M
authors.lastname: Simon
authors.affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Göker
authors.affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
authors.affiliation: Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, Oldenburg, Germany .
authors.forename: Thorsten
authors.initials: T
authors.lastname: Brinkhoff
keywords: Marine; Rhodobacteraceae; Roseobacter clade; facultative anaerobe; methylated compounds; motile