title: Isolation and characterization of 20 polymorphic microsatellite markers for Juglans mandshurica (Juglandaceae).
abstract: OBJECTIVE
  Fifty microsatellite loci were developed for the endangered species Juglans mandshurica to investigate its genetic diversity and population structure. •
  
  
  RESULTS
  In all, 50 microsatellite markers were isolated from J. mandshurica, using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol. Twenty of these polymorphic markers were assessed in samples collected from 98 individuals among five populations in northeastern China. Across all of the J. mandshurica samples, the number of alleles per locus ranged from one to 17. •
  
  
  CONCLUSIONS
  These new microsatellite loci will be useful for conservation genetics studies of J. mandshurica.
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization (Heilongjiang), Northeast Forestry University, Harbin 150040, People's Republic of China.
authors.forename: Chang-Mei
authors.initials: CM
authors.lastname: Chen
authors.affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, Liaoning, People's Republic of China.
authors.forename: Shi-Jie
authors.initials: SJ
authors.lastname: Han
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization (Heilongjiang), Northeast Forestry University, Harbin 150040, People's Republic of China.
authors.forename: Shu-Sheng
authors.initials: SS
authors.lastname: Yuan
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization (Heilongjiang), Northeast Forestry University, Harbin 150040, People's Republic of China.
authors.forename: Chun-Jing
authors.initials: CJ
authors.lastname: Wang
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization (Heilongjiang), Northeast Forestry University, Harbin 150040, People's Republic of China.
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Yu
keywords: Juglandaceae; Juglans mandshurica; genetic conservation; microsatellite marker; simple sequence repeat