title: Estimating the nucleotide diversity in Ceratodon purpureus (Ditrichaceae) from 218 conserved exon-primed, intron-spanning nuclear loci.
abstract: OBJECTIVE
  We developed and tested primers for 218 nuclear loci for studying population genetics, phylogeography, and genome evolution in bryophytes. •
  
  
  RESULTS
  We aligned expressed sequence tags (ESTs) from Ceratodon purpureus to the Physcomitrella patens genome sequence, and designed primers that are homologous to conserved exons but span introns in the P. patens genome. We tested these primers on four isolates from New York, USA; Otavalo, Ecuador; and two laboratory isolates from Austria (WT4 and GG1). The median genome-wide nucleotide diversity was 0.008 substitutions/site, but the range was large (0-0.14), illustrating the among-locus heterogeneity in the species. •
  
  
  CONCLUSIONS
  These loci provide a valuable resource for finely resolved, genome-wide population genetic and species-level phylogenetic analyses of C. purpureus and its relatives.
authors.affiliation: Biology Department, University of Florida, Gainesville, Florida 32611 USA ; Biology Department, Washington University in St. Louis, St. Louis, Missouri 63130 USA.
authors.forename: Stuart F
authors.initials: SF
authors.lastname: McDaniel
authors.affiliation: Center for Genome Sciences, Washington University in St. Louis, St. Louis, Missouri 63130 USA.
authors.forename: Marijke J
authors.initials: MJ
authors.lastname: van Baren
authors.affiliation: Biology Department, University of Florida, Gainesville, Florida 32611 USA.
authors.forename: Kelly S
authors.initials: KS
authors.lastname: Jones
authors.affiliation: Biology Department, University of Florida, Gainesville, Florida 32611 USA.
authors.forename: Adam C
authors.initials: AC
authors.lastname: Payton
authors.affiliation: Biology Department, Washington University in St. Louis, St. Louis, Missouri 63130 USA.
authors.forename: Ralph S
authors.initials: RS
authors.lastname: Quatrano
keywords: Physcomitrella patens; chromosomal inversion; expressed sequence tag (EST); phylogeography; sex-linked loci