title: Millisecond duration pulses for flow-through electro-induced protein extraction from E. coli and associated eradication.
abstract: Pulsed electric fields are used to induce membrane permeabilization on cells. In the case of species with cell wall (yeasts, microalgae), it was previously shown that when the pulse duration was several ms long, this resulted in a cytoplasmic soluble protein slow leakage. In this work, we show that a similar consequence can be obtained with different strains of E. coli. Experimental evidences of a resulting wall alteration are described. Pre-industrial flow process pilots are used. As the membrane electropermeabilization can be irreversible by applying a proper choice of the pulse parameters, this approach is used for bacterial inactivation in flow process. It is observed that sub-millisecond pulse trains are more cost effective than longer ones.
authors.affiliation: CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), 205 route de Narbonne BP64182, F-31077 Toulouse, France; Université de Toulouse, UPS, IPBS, F-31077 Toulouse, France.
authors.forename: M
authors.initials: M
authors.lastname: Coustets
authors.affiliation: CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), 205 route de Narbonne BP64182, F-31077 Toulouse, France; Université de Toulouse, UPS, IPBS, F-31077 Toulouse, France; Sofia University, Dept. Biophysics and Radiobiology, Biological Faculty, 8 Dragan Tzankov blvd, 1164 Sofia, Bulgaria.
authors.forename: V
authors.initials: V
authors.lastname: Ganeva
authors.affiliation: Sofia University, Dept. Biophysics and Radiobiology, Biological Faculty, 8 Dragan Tzankov blvd, 1164 Sofia, Bulgaria.
authors.forename: B
authors.initials: B
authors.lastname: Galutzov
authors.affiliation: CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), 205 route de Narbonne BP64182, F-31077 Toulouse, France; Université de Toulouse, UPS, IPBS, F-31077 Toulouse, France. Electronic address: Justin.teissie@ipbs.fr.
authors.forename: J
authors.initials: J
authors.lastname: Teissie
mesh_terms: D002021:Buffers; D002462:Cell Membrane / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D018274:Electroporation / Q000379:methods; D004926:Escherichia coli / Q000737:chemistry / Q000254:growth & development; D029968:Escherichia coli Proteins / Q000302:isolation & purification*; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000302:isolation & purification; D009113:Muramidase / Q000737:chemistry; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D013696:Temperature
chemical_list: D002021:Buffers; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); D009113:Muramidase
keywords: E. coli; Electropulsation; Extraction; Flow process; Inactivation