title: Antioxidant and anti-acetylcholinesterase activities of extracts and secondary metabolites from Acacia cyanophylla.
abstract: OBJECTIVE
  To investigate the antioxidant potential and anti-acetycholinesterase activity of compounds and extracts from Acacia cyanophylla (A. cyanophylla).
  
  
  METHODS
  Three polyphenolic compounds were isolated from ethyl acetate extract of A. cyanophylla flowers. They have been identified as isosalipurposide 1, quercetin 2 and naringenin 3. Their structures were elucidated by extensive spectroscopic methods including 1D and 2D NMR experiments as well as ES-MS. The prepared extracts and the isolated compounds 1-3 were tested for their antioxidant activity using 1'-1'-diphenylpicrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) scavenging assays and reducing power. They have been also investigated for inhibitory effect against acetylcholinesterase using the microplate assay.
  
  
  RESULTS
  In the DPPH test, the EtOAc extract of flowers exhibited the highest antioxidant effect (67.26 µg/mL). Isosalipurposide 1 showed a significant antiradical power against DPPH (81.9 µg/mL). All extracts showed a dose-dependent acetylcholinesterase inhibition. In terms of the IC50 value, the butanolic extract (16.03 µg/mL) was the most potent sample. Isosalipurposide 1 was found to be active against AChE with an IC50 value of 52.04 µg/mL.
  
  
  CONCLUSIONS
  The results demonstrated the important antioxidant and anti-acetylcholinesterase activity of pure compounds and extracts from A. cyanophylla.
authors.affiliation: Laboratoire de Chimie hétérocyclique, Produits Naturels et Réactivité. Equipe: Chimie Médicinale et Produits Naturels. Faculté des Sciences de Monastir, Université de Monastir 5019, Monastir, Tunisie.
authors.forename: Lotfi
authors.initials: L
authors.lastname: Ghribia
authors.affiliation: Laboratoire de Chimie hétérocyclique, Produits Naturels et Réactivité. Equipe: Chimie Médicinale et Produits Naturels. Faculté des Sciences de Monastir, Université de Monastir 5019, Monastir, Tunisie.
authors.forename: Hatem
authors.initials: H
authors.lastname: Ghouilaa
authors.affiliation: Laboratoire des Maladies Transmissibles et des Substances Biologiquement Actives, Faculté de Pharmacie, 5000 Monastir, Tunisie.
authors.forename: Amel
authors.initials: A
authors.lastname: Omrib
authors.affiliation: Laboratoire des Maladies Transmissibles et des Substances Biologiquement Actives, Faculté de Pharmacie, 5000 Monastir, Tunisie.
authors.forename: Malek
authors.initials: M
authors.lastname: Besbesb
authors.affiliation: Laboratoire de Chimie hétérocyclique, Produits Naturels et Réactivité. Equipe: Chimie Médicinale et Produits Naturels. Faculté des Sciences de Monastir, Université de Monastir 5019, Monastir, Tunisie.
authors.forename: Hichem Ben
authors.initials: HB
authors.lastname: Janneta
keywords: Acacia cyanophylla; Anti-acetylcholinesterase activity; Antioxidant activity; Isosalipurposide; Naringenin; Quercetin