title: Molecular Characterization of Human and Animal Isolates of Echinococcus granulosus in the Thrace Region, Turkey.
abstract: OBJECTIVE
  Echinococcus granulosus is the causative agent of cystic echinococcosis in humans and many domestic animals, and remains an important global health problem. The aim of this study was to genotype E. granulosus isolates obtained from humans and animals in the Thrace Region of Turkey.
  
  
  METHODS
  A total of 58 isolates were obtained from patients who underwent surgery at several hospitals and from animals at a slaughterhouse in the province of Edirne. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of ribosomal internal transcribed spacer 1 fragments, and polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) of the partial mitochondrial NADH dehydrogenase subunit 1 (ND1) gene, was used to characterize human and animal E. granulosus isolates. To investigate the genetic characteristics of isolates, deoxyribonucleic acid (DNA) sequencing of the mitochondrial cytochrome c oxidase subunit 1 (CO1) and ND1 genes was performed.
  
  
  RESULTS
  Fifty-eight E. granulosus isolates, including 42 from human, 13 from cattle and 3 from sheep were, analyzed. The results indicated two distinct genotypes: the G1 (sheep strain) and G7 (pig strain) genotypes. The sheep strain was shown to be the most common genotype of E. granulosus affecting humans, sheep and cattle. Among the concatenated partial CO1 and ND1 sequence data, eight haplotypes of Echinococcus species were identified in the present study.
  
  
  CONCLUSIONS
  This is the first report indicating that the E. granulosus pig strain is present in humans in this region. We suggest that new strategies be designed for E. granulosus control programs in Turkey.
authors.affiliation: Clinic of Medical Microbiology, Tokat State Hospital, Tokat, Turkey.
authors.forename: Canan
authors.initials: C
authors.lastname: Eryıldız
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Trakya University, Edirne, Turkey.
authors.forename: Nermin
authors.initials: N
authors.lastname: Sakru
keywords: CO1; Echinococcus granulosus; Edirne; ND1; PCR-RFLP; PCR-SSCP; Thrace region; Turkey; genotype