title: Distribution of aquaporin genes and selection of individual reference genes for quantitative real-time RT-PCR analysis in multiple tissues of the mouse.
abstract: Aquaporins (AQPs) are a family of water-transporting proteins that are selectively expressed in epithelial, endothelial, and many other cell types of various tissues, where they play important physiological functions. However, the accurate distribution of AQP gene expression has not yet been examined in various tissues of the mouse. We first evaluated the tissue distribution of AQP gene expression using tongue, nasal epithelium, bronchus, trachea, lung, esophagus, stomach, ileum, transverse colon, liver, pancreas, whole blood, thigh muscle, spinal cord, brain, thoracic aorta, heart, kidney, thymus, spleen, skin, eye, and testis of the mouse. Furthermore, for a quantitative analysis, we selected appropriate reference genes for normalized qRT-PCR data in various tissues. The stability of the reference genes was assessed using NormFinder. The stably expressed genes identified in the present study were 18s rRNA. When 18s rRNA was used, as the best reference gene in the present study, the genes for AQPs 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 11, and 12 were notably expressed in the eye, lung, testis, eye, spinal cord, trachea, kidney, testis, testis, testis, testis, and pancreas. These results, regarding the distribution of AQPs, suggest that AQPs may be involved in various physiological and pathophysiological processes.
authors.affiliation: a Division of Pharmacy Professional Development & Research, School of Pharmacy, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Sakai
authors.forename: Ken
authors.initials: K
authors.lastname: Sato
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kai
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Shoji
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hasegawa
authors.forename: Maiko
authors.initials: M
authors.lastname: Nishizaki
authors.forename: Atsunobu
authors.initials: A
authors.lastname: Sagara
authors.forename: Akira
authors.initials: A
authors.lastname: Yamashita
authors.forename: Minoru
authors.initials: M
authors.lastname: Narita
mesh_terms: D000818:Animals; D020346:Aquaporins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005819:Genetic Markers; D008297:Male; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D012337:RNA, Ribosomal, 18S / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D020346:Aquaporins; D005819:Genetic Markers; D012337:RNA, Ribosomal, 18S
keywords: NormFinder; Normfinder; distribution tissulaire des aquaporines; gènes de référence; reference genes; tissue distribution of aquaporin