title: Development of 12 chloroplast microsatellite markers in Vigna unguiculata (Fabaceae) and amplification in Phaseolus vulgaris.
abstract: OBJECTIVE
  Vigna unguiculata is an economically important legume, and the complexity of its variability and evolution needs to be further understood. Based on publicly available databases, we developed chloroplast microsatellite primers to investigate genetic diversity within V. unguiculata and its related species Phaseolus vulgaris. •
  
  
  RESULTS
  Twelve polymorphic chloroplast microsatellite markers were developed and characterized in 62 V. unguiculata individuals. The number of alleles per locus varied between two and four, the unbiased haploid diversity per locus ranged from 0.123 to 0.497, and the polymorphism information content varied from 0.114 to 0.369. In cross-species amplifications, nine of these markers showed polymorphism in 29 P. vulgaris individuals. •
  
  
  CONCLUSIONS
  The newly developed chloroplast microsatellite markers exhibit variation in V. unguiculata as well as their transferability in P. vulgaris. These markers can be used to investigate genetic diversity and evolution in V. unguiculata and P. vulgaris.
authors.affiliation: School of Life Sciences, Jianghan University, Wuhan 430056, Hubei, People's Republic of China ; Hubei Province Engineering Research Center of Legume Plants, Jianghan University, Wuhan 430056, Hubei, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Pan
authors.affiliation: School of Life Sciences, Jianghan University, Wuhan 430056, Hubei, People's Republic of China ; Hubei Province Engineering Research Center of Legume Plants, Jianghan University, Wuhan 430056, Hubei, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Life Sciences, Jianghan University, Wuhan 430056, Hubei, People's Republic of China ; Hubei Province Engineering Research Center of Legume Plants, Jianghan University, Wuhan 430056, Hubei, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.affiliation: School of Life Sciences, Jianghan University, Wuhan 430056, Hubei, People's Republic of China ; Hubei Province Engineering Research Center of Legume Plants, Jianghan University, Wuhan 430056, Hubei, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wu
authors.affiliation: School of Life Sciences, Jianghan University, Wuhan 430056, Hubei, People's Republic of China ; Hubei Province Engineering Research Center of Legume Plants, Jianghan University, Wuhan 430056, Hubei, People's Republic of China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Hu
authors.affiliation: School of Life Sciences, Jianghan University, Wuhan 430056, Hubei, People's Republic of China ; Hubei Province Engineering Research Center of Legume Plants, Jianghan University, Wuhan 430056, Hubei, People's Republic of China.
authors.forename: Chanyou
authors.initials: C
authors.lastname: Chen
keywords: Fabaceae; Phaseolus vulgaris; Vigna unguiculata; chloroplast microsatellite; cross-amplification