title: Development of microsatellite markers for two Australian Persoonia (Proteaceae) species using two different techniques.
abstract: OBJECTIVE
  Microsatellite markers were developed and cross-species transferability assessed for two Persoonia species to evaluate genetic diversity and population genetic structure of these broadly distributed southwest Australian tree species. •
  
  
  RESULTS
  Microsatellite-enriched libraries and 454 GS-FLX shotgun sequencing were used to identity nine microsatellite loci for P. elliptica (one 454; eight cloning) and six for P. longifolia (three 454; three cloning). These loci were screened for variation in individuals from populations in southwestern Australia. In P. elliptica, observed and expected heterozygosities ranged from 0.46 to 0.93 and 0.42 to 0.88, respectively. For P. longifolia, observed and expected heterozygosities ranged from 0.04 to 0.88 and 0.04 to 0.84, respectively. •
  
  
  CONCLUSIONS
  The microsatellites identified in this study will enable the examination of population and spatial structuring of genetic diversity in P. elliptica and P. longifolia, two priority species for mine site restoration in southwestern Australia.
authors.affiliation: School of Plant Biology, University of Western Australia, Nedlands, Western Australia 6009, Australia ; Botanic Gardens and Parks Authority, Kings Park and Botanic Garden, West Perth, Western Australia 6005, Australia.
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Stingemore
authors.affiliation: School of Plant Biology, University of Western Australia, Nedlands, Western Australia 6009, Australia ; Botanic Gardens and Parks Authority, Kings Park and Botanic Garden, West Perth, Western Australia 6005, Australia.
authors.forename: Paul G
authors.initials: PG
authors.lastname: Nevill
authors.affiliation: School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, South Australia 5001, Australia ; Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, South Australia 5000, Australia.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Gardner
authors.affiliation: School of Plant Biology, University of Western Australia, Nedlands, Western Australia 6009, Australia ; Botanic Gardens and Parks Authority, Kings Park and Botanic Garden, West Perth, Western Australia 6005, Australia.
authors.forename: Siegfried L
authors.initials: SL
authors.lastname: Krauss
keywords: 454 GS-FLX; Persoonia; Proteaceae; cross-species amplification; microsatellites; southwest Australia