title: Buyang Huanwu Decoction fraction protects against cerebral ischemia/reperfusion injury by attenuating the inflammatory response and cellular apoptosis.
abstract: Buyang Huanwu Decoction fraction extracted from Buyang Huanwu Decoction contains saponins of Astragalus, total paeony glycoside and safflower flavones. The aim of this study was to demonstrate the neuroprotective effect and mechanism of Buyang Huanwu Decoction fraction on ischemic injury both in vivo and in vitro. In vivo experiments showed that 50-200 mg/kg Buyang Huanwu Decoction fraction reduced infarct volume and pathological injury in ischemia/reperfusion rats, markedly inhibited expression of nuclear factor-κB and tumor necrosis factor-α and promoted nestin protein expression in brain tissue. Buyang Huanwu Decoction fraction (200 mg/kg) exhibited significant effects, which were similar to those of 100 mg/kg Ginkgo biloba extract. In vitro experimental results demonstrated that 10-100 mg/L Buyang Huanwu Decoction fraction significantly improved cell viability, decreased the release of lactate dehydrogenase and malondialdehyde levels, and inhibited the rate of apoptosis in HT22 cells following oxygen-glucose deprivation. Buyang Huanwu Decoction fraction (100 mg/L) exhibited significant effects, which were similar to those of 100 mg/L Ginkgo biloba extract. These findings suggest that Buyang Huanwu Decoction fraction may represent a novel, protective strategy against cerebral ischemia/reperfusion injury in rats and oxygen-glucose deprivation-induced damage in HT22 cells in vitro by attenuating the inflammatory response and cellular apoptosis.
authors.affiliation: Department of Pharmacology, Key Laboratory of Anti-inflammatory and Immunopharmacology, Ministry of Education; Key Laboratory of Chinese Medicine Research and Development, State Administration of Traditional Chinese Medicine, Anhui Medical University, Hefei 230032, Anhui Province, China ; Anhui Provincial Children's Hospital, Hefei 230051, Anhui Province, China.
authors.forename: Yulian
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Pharmacology, Key Laboratory of Anti-inflammatory and Immunopharmacology, Ministry of Education; Key Laboratory of Chinese Medicine Research and Development, State Administration of Traditional Chinese Medicine, Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: Liuyi
authors.initials: L
authors.lastname: Dong
authors.affiliation: Department of Pharmacology, Key Laboratory of Anti-inflammatory and Immunopharmacology, Ministry of Education; Key Laboratory of Chinese Medicine Research and Development, State Administration of Traditional Chinese Medicine, Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: Changqing
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Pharmacology, Key Laboratory of Anti-inflammatory and Immunopharmacology, Ministry of Education; Key Laboratory of Chinese Medicine Research and Development, State Administration of Traditional Chinese Medicine, Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Qin
authors.affiliation: Department of Pharmacology, Key Laboratory of Anti-inflammatory and Immunopharmacology, Ministry of Education; Key Laboratory of Chinese Medicine Research and Development, State Administration of Traditional Chinese Medicine, Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Li
authors.affiliation: Anhui Institute of Materia Medica, Hefei 230022, Anhui Province, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Hefei Qi-xing Medicine and Technology Co., Ltd., Hefei 230088, Anhui Province, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Shao
authors.affiliation: Synthetic Laboratory of Basic Medicine College, Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: Dake
authors.initials: D
authors.lastname: Huang
keywords: Buyang Huanwu Decoction fraction; apoptosis; brain injury; grants-supported paper; hippocampus; inflammatory reaction; ischemia/reperfusion injury; neural regeneration; neurons; neuroprotection; neuroregeneration; oxidation; photographs-containing paper; traditional Chinese herbal medicines; traditional Chinese medicine