title: Expression of the brother of the regulator of imprinted sites gene in the sputum of patients with lung cancer.
abstract: BACKGROUND
  Brother of the regulator of imprinted sites (BORIS) is a putative new oncogene that is classified as a cancer germline gene; however, its role in the development of cancer is unclear. This study investigated the expression of BORIS in lung cancer and its clinical implications.
  
  
  METHODS
  The expression of BORIS messenger ribonucleic acid (mRNA) in the sputum of 100 patients with lung cancer (50 with squamous cell carcinoma, 36 with adenocarcinoma, and 14 with small-cell carcinoma) was evaluated by reverse transcription polymerase chain reaction.
  
  
  RESULTS
  The overall expression rate of BORIS in patients with lung cancer was 36.0%: 19 of 50 squamous cell carcinomas (38.0%), 13 of 36 adenocarcinomas (36.1%), and 4 of 14 (28.6%) small-cell carcinomas. There was no significant difference in the BORIS expression according to age, gender, or histologic type. However, the mRNA expression of BORIS was significantly related to the pathologic cancer stage (p=0.004) and lymph node metastasis (p=0.001). The expression of the melanoma antigen gene family A1-6 was not associated with the expression of BORIS.
  
  
  CONCLUSIONS
  Our results suggest that the expression of BORIS might be a negative prognostic factor in lung cancers and implicate BORIS as a molecular target for immunotherapy.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Kosin University Gospel Hospital, Kosin University College of Medicine.
authors.forename: Hae Young
authors.initials: HY
authors.lastname: Lee
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Kosin University Gospel Hospital, Kosin University College of Medicine.
authors.forename: Jong In
authors.initials: JI
authors.lastname: Kim
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Kosin University Gospel Hospital, Kosin University College of Medicine.
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Cho
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Kosin University Gospel Hospital, Kosin University College of Medicine.
authors.forename: Taek Yong
authors.initials: TY
authors.lastname: Ko
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Kosin University Gospel Hospital, Kosin University College of Medicine.
authors.forename: Hyun Su
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Kosin University Gospel Hospital, Kosin University College of Medicine.
authors.forename: Sung Dal
authors.initials: SD
authors.lastname: Park
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Kosin University Gospel Hospital, Kosin University College of Medicine.
authors.forename: Sung Rae
authors.initials: SR
authors.lastname: Cho
authors.affiliation: Department of Pathology, Kosin University Gospel Hospital, Kosin University College of Medicine.
authors.forename: Hee Kyung
authors.initials: HK
authors.lastname: Chang
authors.affiliation: Department of Familly Medicine, Kosin University Gospel Hospital, Kosin University College of Medicine.
authors.forename: Guk Jin
authors.initials: GJ
authors.lastname: Hwang
authors.affiliation: Department of Clinical Laboratory Science, Gimhae College.
authors.forename: Sang Bong
authors.initials: SB
authors.lastname: Jung
keywords: Brother of the regulator of imprinted sites; Lung neoplasms; Polymerase chain reaction; Reverse transcription