title: Epigallocatechin-3-gallate protects against cisplatin nephrotoxicity by inhibiting the apoptosis in mouse.
abstract: Cisplatin (CP) is a commonly used anticancer drug, but its notable side effect of nephrotoxicity limits its use in clinic. Epigallocatechin-3-gallate (EGCG), an anti-oxidant, anti-inflammatory, and anti-tumorigenic green tea polyphenol, has been available on the market for its beneficial effects. The aim of this study was to investigate whether EGCG can prevent the nephrotoxic effect of CP and the involved mechanisms. Male C57/BL6 mice were randomly divided into four groups: control group, EGCG group, CP group, and CP+EGCG group. On day 5, mice were sacrificed. Our results showed that EGCG treatment significantly ameliorated the histopathological changes and the increased serum creatinine and blood urea nitrogen (BUN) induced by CP. TUNEL-positive cells significantly reduced in the CP+EGCG group compared with CP group. EGCG also inhibited the expression of the ligand of death receptor Fas (Fas-L), apoptosis regulator BAX (Bax) and tumor-suppressor protein p53, and increased the expression of B-cell lymphoma 2 (Bcl-2). These findings suggest that EGCG can ameliorate CP-induced apoptosis in the kidney by regulating death receptor Fas conducted extrinsic pathway, and the expression of Bax and Bcl-2.
authors.affiliation: Department of Nephrology, Qi Lu Hospital of Shandong University Jinan, Shandong 250012, China.
authors.forename: Peimei
authors.initials: P
authors.lastname: Zou
authors.affiliation: Department of Nephrology, Qi Lu Hospital of Shandong University Jinan, Shandong 250012, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Nephrology, Qi Lu Hospital of Shandong University Jinan, Shandong 250012, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Department of Nephrology, Qi Lu Hospital of Shandong University Jinan, Shandong 250012, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Pei
authors.affiliation: Department of Nephrology, Qi Lu Hospital of Shandong University Jinan, Shandong 250012, China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Nephrology, Qi Lu Hospital of Shandong University Jinan, Shandong 250012, China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Nephrology, Qi Lu Hospital of Shandong University Jinan, Shandong 250012, China ; Ministry of Education Key Laboratory of Experimental Teratology and Institute of Molecular Medicine and Genetics, Shandong University School of Medicine Jinan, Shandong 250013, China.
authors.forename: Guangyi
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Nephrology, Qi Lu Hospital of Shandong University Jinan, Shandong 250012, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002945:Cisplatin / Q000633:toxicity*; D004195:Disease Models, Animal; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D002392:Catechin; C045651:epigallocatechin gallate; D002945:Cisplatin
keywords: Cisplatin (CP); Epigallocatechin-3-gallate (EGCG); apoptosis; mouse; renal