title: Sophora microphylla (Fabaceae) microsatellite markers and their utility across the genus.
abstract: OBJECTIVE
  Genus-specific microsatellite markers were developed for Sophora for population genetic and systematic studies of the group in New Zealand, and potentially elsewhere in the geographic range. •
  
  
  RESULTS
  From sequencing a total genomic DNA library (using Roche 454), we identified and developed 29 polymorphic microsatellite markers for S. microphylla and S. chathamica. We tested 12 of these markers on 14 S. chathamica individuals and four S. microphylla populations. All loci amplified in both species and species-specific alleles occurred at seven loci. In S. microphylla populations, the observed and expected heterozygosities ranged from 0.000-0.960 and 0.000-0.908, respectively, with alleles per locus ranging from seven to 23. •
  
  
  CONCLUSIONS
  The developed markers will be valuable in studies of phylogenetics, population structure, mating system, and selection of provenances for restoration projects.
authors.affiliation: Institute of Agriculture and Environment, Massey University, Palmerston North 4442, New Zealand.
authors.forename: Megan L
authors.initials: ML
authors.lastname: Van Etten
authors.affiliation: Landcare Research, Lincoln 7640, New Zealand.
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Houliston
authors.affiliation: Landcare Research, Lincoln 7640, New Zealand.
authors.forename: Caroline M
authors.initials: CM
authors.lastname: Mitchell
authors.affiliation: Landcare Research, Lincoln 7640, New Zealand.
authors.forename: Peter B
authors.initials: PB
authors.lastname: Heenan
authors.affiliation: Institute of Agriculture and Environment, Massey University, Palmerston North 4442, New Zealand.
authors.forename: Alastair W
authors.initials: AW
authors.lastname: Robertson
authors.affiliation: Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Tate
keywords: Fabaceae; Sophora microphylla; genetic variation; simple sequence repeat markers