title: Development and characterization of microsatellite markers for the medicinal plant Smilax brasiliensis (Smilacaceae) and related species.
abstract: OBJECTIVE
  A new set of microsatellite or simple sequence repeat (SSR) markers were developed for Smilax brasiliensis, which is popularly known as sarsaparilla and used in folk medicine as a tonic, antirheumatic, and antisyphilitic. Smilax brasiliensis is sold in Brazilian pharmacies, and its origin and effectiveness are not subject to quality control. •
  
  
  RESULTS
  Using a protocol for genomic library enrichment, primer pairs were developed for 26 microsatellite loci and validated in 17 accessions of S. brasiliensis. Thirteen loci were polymorphic and four were monomorphic. The primers successfully amplified alleles in the congeners S. campestris, S. cissoides, S. fluminensis, S. goyazana, S. polyantha, S. quinquenervia, S. rufescens, S. subsessiliflora, and S. syphilitica. •
  
  
  CONCLUSIONS
  The new SSR markers described herein are informative tools for genetic diversity and gene flow studies in S. brasiliensis and several congeners.
authors.affiliation: Departamento de Ciências Biológicas, Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Av. Pádua Dias 11, 13418-900 Piracicaba, São Paulo, Brazil.
authors.forename: Aline R
authors.initials: AR
authors.lastname: Martins
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Av. Pádua Dias 11, 13400-970 Piracicaba, São Paulo, Brazil.
authors.forename: Aluana G
authors.initials: AG
authors.lastname: Abreu
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Av. Pádua Dias 11, 13400-970 Piracicaba, São Paulo, Brazil.
authors.forename: Miklos M
authors.initials: MM
authors.lastname: Bajay
authors.affiliation: Departmento de Zootecnia, Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Av. Pádua Dias 11, 13418-900 Piracicaba, São Paulo, Brazil.
authors.forename: Priscilla M S
authors.initials: PM
authors.lastname: Villela
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Av. Pádua Dias 11, 13400-970 Piracicaba, São Paulo, Brazil.
authors.forename: Carlos E A
authors.initials: CE
authors.lastname: Batista
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Av. Pádua Dias 11, 13400-970 Piracicaba, São Paulo, Brazil.
authors.forename: Mariza
authors.initials: M
authors.lastname: Monteiro
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Av. Pádua Dias 11, 13400-970 Piracicaba, São Paulo, Brazil.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Alves-Pereira
authors.affiliation: Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agricolas (CPQBA), Universidade Estadual de Campinas (UNICAMP), C.P. 6171, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Glyn M
authors.initials: GM
authors.lastname: Figueira
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Av. Pádua Dias 11, 13400-970 Piracicaba, São Paulo, Brazil.
authors.forename: José B
authors.initials: JB
authors.lastname: Pinheiro
authors.affiliation: Departamento de Ciências Biológicas, Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Av. Pádua Dias 11, 13418-900 Piracicaba, São Paulo, Brazil.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Appezzato-da-Glória
authors.affiliation: Agência Paulista de Tecnologia dos Agronegócios, Rodovia SP 127, Vila Fátima, 13400-970 Piracicaba, São Paulo, Brazil.
authors.forename: Maria I
authors.initials: MI
authors.lastname: Zucchi
keywords: Smilax; medicinal plant; microsatellites; sarsaparilla; transferability