title: Influence of circadian time and lighting conditions on expression of melatonin receptors 1 and 2 in murine lymphocytes.
abstract: OBJECTIVE
  To investigate the expression of membrane melatonin receptors in murine thymocytes, CD4(+) T-cells, bone marrow cells and B-cells according to melatonin concentration in the blood.
  
  
  METHODS
  The levels of mRNA of melatonin receptors were investigated in the cells isolated during the day or during the night, corresponding to low and high melatonin concentrations in the blood.
  
  
  RESULTS
  Low levels of Mtnr1 and Mtnr2 transcripts were detected in thymocytes and splenic B-cells. The expression of membranous melatonin receptors in B-cells corresponds to melatonin concentration in the blood.
  
  
  CONCLUSIONS
  The expression of Mtnr1 and Mtnr2 in murine lymphocytes is very weak. Melatonin may be involved in regulation of the expression of Mtnr1 and Mtnr2 in murine B-cells.
authors.affiliation: State Research Institute Centre for Innovative Medicine, Department of immunology, Vilnius, Lithuania Department of Microbiology and Biotechnology, Faculty of Natural Sciences, Vilnius University, Vilnius, Lithuania.
authors.forename: Vitalij
authors.initials: V
authors.lastname: Cernysiov
authors.affiliation: State Research Institute Centre for Innovative Medicine, Department of immunology, Vilnius, Lithuania.
authors.forename: Ruta
authors.initials: R
authors.lastname: Bozaite
authors.affiliation: State Research Institute Centre for Innovative Medicine, Department of immunology, Vilnius, Lithuania.
authors.forename: Mykolas
authors.initials: M
authors.lastname: Mauricas
authors.affiliation: State Research Institute Centre for Innovative Medicine, Department of immunology, Vilnius, Lithuania igirkontaite@yahoo.com.
authors.forename: Irute
authors.initials: I
authors.lastname: Girkontaite
mesh_terms: D000818:Animals; D002940:Circadian Rhythm; D005786:Gene Expression Regulation; D008027:Light; D008029:Lighting; D008214:Lymphocytes / Q000378:metabolism*; D008550:Melatonin / Q000097:blood / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D017440:Photoperiod; D044122:Receptor, Melatonin, MT1 / Q000235:genetics* / Q000378:metabolism; D044123:Receptor, Melatonin, MT2 / Q000235:genetics* / Q000378:metabolism; D044094:Receptors, Melatonin / Q000235:genetics*
chemical_list: D044122:Receptor, Melatonin, MT1; D044123:Receptor, Melatonin, MT2; D044094:Receptors, Melatonin; D008550:Melatonin
keywords: Melatonin; Mtnr1; Mtnr2; lymphocytes; mouse