title: Potential therapeutic roles of tanshinone IIA in human bladder cancer cells.
abstract: Tanshinone IIA (Tan-IIA), one of the major lipophilic components isolated from the root of Salviae Miltiorrhizae, has been found to exhibit anticancer activity in various cancer cells. We have demonstrated that Tan-IIA induces apoptosis in several human cancer cells through caspase- and mitochondria-dependent pathways. Here we explored the anticancer effect of Tan-IIA in human bladder cancer cell lines. Our results showed that Tan-IIA caused bladder cancer cell death in a time- and dose-dependent manner. Tan-IIA induced apoptosis through the mitochondria-dependent pathway in these bladder cancer cells. Tan-IIA also suppressed the migration of bladder cancer cells as revealed by the wound healing and transwell assays. Finally, combination therapy of Tan-IIA with a lower dose of cisplatin successfully killed bladder cancer cells, suggesting that Tan-IIA can serve as a potential anti-cancer agent in bladder cancer.
authors.affiliation: Department of Research, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, No. 88, Section 1, Fengxing Road, Tanzi Dist., Taichung 427, Taiwan. fantac10@gmail.com.
authors.forename: Sheng-Chun
authors.initials: SC
authors.lastname: Chiu
authors.affiliation: Department of Ophthalmology, Mackay Memorial Hospital, No. 690, Section 2, Guangfu Road, East Dist., Hsinchu 30071, Taiwan. hopes929@yahoo.com.tw.
authors.forename: Sung-Ying
authors.initials: SY
authors.lastname: Huang
authors.affiliation: Department of Research, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, No. 88, Section 1, Fengxing Road, Tanzi Dist., Taichung 427, Taiwan. fantac10@gmail.com.
authors.forename: Shu-Fang
authors.initials: SF
authors.lastname: Chang
authors.affiliation: Tzu Chi Stem Cells Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, No. 707, Section 3, Chung-Yang Road, Hualien 970, Taiwan. asdinap@yahoo.com.tw.
authors.forename: Shee-Ping
authors.initials: SP
authors.lastname: Chen
authors.affiliation: Department of Urology, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, No. 88, Section 1, Fengxing Road, Tanzi Dist., Taichung 427, Taiwan. kukoc0925@gmail.com.
authors.forename: Chi-Cheng
authors.initials: CC
authors.lastname: Chen
authors.affiliation: Department of Urology, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, No. 88, Section 1, Fengxing Road, Tanzi Dist., Taichung 427, Taiwan. thlin@hotmail.com.
authors.forename: Tien-Huang
authors.initials: TH
authors.lastname: Lin
authors.affiliation: Department of Urology, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, No. 88, Section 1, Fengxing Road, Tanzi Dist., Taichung 427, Taiwan. d98631006@ntu.edu.tw.
authors.forename: Hsin-Ho
authors.initials: HH
authors.lastname: Liu
authors.affiliation: Department of Urology, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, No. 88, Section 1, Fengxing Road, Tanzi Dist., Taichung 427, Taiwan. zoneshin@yahoo.com.tw.
authors.forename: Tsung-Hsun
authors.initials: TH
authors.lastname: Tsai
authors.affiliation: Department of Urology, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, No. 88, Section 1, Fengxing Road, Tanzi Dist., Taichung 427, Taiwan. j520037@yahoo.com.tw.
authors.forename: Shang-Sen
authors.initials: SS
authors.lastname: Lee
authors.affiliation: Department of Medical Research, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, No. 707, Section 3, Chung-Yang Road, Hualien 970, Taiwan. cypang@mail.tcu.edu.tw.
authors.forename: Cheng-Yoong
authors.initials: CY
authors.lastname: Pang
authors.affiliation: Department of Urology, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, No. 88, Section 1, Fengxing Road, Tanzi Dist., Taichung 427, Taiwan. pchdf95@gmail.com.
authors.forename: Teng-Fu
authors.initials: TF
authors.lastname: Hsieh
mesh_terms: D045784:Abietanes / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D045744:Cell Line, Tumor; D002465:Cell Movement; D002945:Cisplatin / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D006801:Humans; D001749:Urinary Bladder Neoplasms / Q000378:metabolism*
chemical_list: D045784:Abietanes; D000970:Antineoplastic Agents; C021751:tanshinone; D002945:Cisplatin