title: High-throughput microsatellite marker development for the distylous herb Primula mistassinica (Primulaceae).
abstract: OBJECTIVE
  Twelve microsatellite markers were developed for Primula mistassinica, a distylous, diploid arctic-alpine plant. The markers will be used to investigate the landscape genetics of a disjunct population on Isle Royale, Michigan, and the phylogeographic patterns of the species. •
  
  
  RESULTS
  We used Roche/454 high-throughput technology to sequence microsatellite-enriched regions in the P. mistassinica genome. We developed 12 polymorphic microsatellite primer sets. These loci contained di-, tri-, and tetranucleotide repeats with two to nine alleles per locus when assessed in 23 individuals. •
  
  
  CONCLUSIONS
  Understanding the historical movements of P. mistassinica will provide insight to the survival prospects of current Arctic plant populations, which face the pressures of global, anthropogenic climate change.
authors.affiliation: Department of Biology, Williams College, Williamstown, Massachusetts 01267 USA.
authors.forename: Hannah
authors.initials: H
authors.lastname: Matheny
authors.affiliation: Department of Biology, Williams College, Williamstown, Massachusetts 01267 USA.
authors.forename: Joan
authors.initials: J
authors.lastname: Edwards
authors.affiliation: Department of Biology, Williams College, Williamstown, Massachusetts 01267 USA.
authors.forename: Luana S
authors.initials: LS
authors.lastname: Maroja
keywords: 454 sequencing; Primula mistassinica; Primulaceae; arctic-alpine plant; microsatellite enrichment; polymorphism