title: Possible involvement of CA1 5-HT1B/1D and 5-HT2A/2B/2C receptors in harmaline-induced amnesia.
abstract: In the present study, effects of the serotonergic system of the dorsal hippocampus (CA1) on harmaline-induced amnesia were examined. A single-trial step-down passive avoidance task was used for the assessment of memory retention in adult male NMRI mice. Pre-training intra-peritoneal (i.p.) administration of harmaline (1mg/kg) induced impairment of memory retention. Moreover, intra-CA1 administration of 5-HT1B/1D receptor agonist, CP94253 (5 ng/mouse), 5-HT1B/1D receptor antagonist, GR127935 (0.05 and 0.5 ng/mouse), 5-HT2A/2B/2C receptor agonist, α-methyl 5-HT (0.5 ng/mouse) and 5-HT2 receptor antagonist, cinancerine (0.5 ng/mouse) impaired memory acquisition, but did not affect locomotor activity and tail flick. Furthermore, pre-training intra-CA1 injection of subthreshold dose of CP94253 (0.05 ng/mouse) or GR127935 (0.005 ng/mouse) reversed impairment of memory acquisition induced by harmaline (1 mg/kg, i.p.). However, pre-training intra-CA1 infusion of subthreshold dose of α-methyl 5-HT (0.005 ng/mouse) or cinancerine (0.005 ng/mouse) with the administration of harmaline (0.5 and 1 mg/kg, i.p.) heighten impairment of memory acquisition. These findings implicate the involvement of CA1 serotonergic mechanism in harmaline-induced impairment of memory acquisition.
authors.affiliation: School of Advanced Sciences in Medicine, Islamic Azad University, Tehran Medical Branch, Tehran, Iran. Electronic address: Nasehi@iricss.org.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nasehi
authors.affiliation: Department of Biology, Faculty of Basic Science, Tarbiat Moalem (Kharazmi) University, Tehran, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Jamshidi-Mehr
authors.affiliation: Institute for Cognitive Science Studies (ICSS), Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Khakpai
authors.affiliation: School of Advanced Sciences in Medicine, Islamic Azad University, Tehran Medical Branch, Tehran, Iran; Institute for Cognitive Science Studies (ICSS), Tehran, Iran; Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology and Iranian National Center for Addiction Studies, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; School of Cognitive Sciences, Institute for Research in Fundamental Sciences (IPM), Tehran, Iran. Electronic address: zarinmr@ams.ac.ir.
authors.forename: Mohammad-Reza
authors.initials: MR
authors.lastname: Zarrindast
mesh_terms: D000647:Amnesia / Q000139:chemically induced*; D000818:Animals; D056547:CA1 Region, Hippocampal / Q000187:drug effects* / Q000502:physiology; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D002928:Cinanserin / Q000494:pharmacology; D006246:Harmaline / Q000494:pharmacology*; D008297:Male; D008568:Memory / Q000187:drug effects; D008815:Mice, Inbred Strains; D010069:Oxadiazoles / Q000494:pharmacology; D010879:Piperazines / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D044302:Receptor, Serotonin, 5-HT1B / Q000187:drug effects* / Q000502:physiology; D044346:Receptor, Serotonin, 5-HT1D / Q000187:drug effects* / Q000502:physiology; D044402:Receptor, Serotonin, 5-HT2A / Q000187:drug effects* / Q000502:physiology; D044403:Receptor, Serotonin, 5-HT2B / Q000187:drug effects* / Q000502:physiology; D044405:Receptor, Serotonin, 5-HT2C / Q000187:drug effects* / Q000502:physiology; D012701:Serotonin / Q000031:analogs & derivatives / Q000494:pharmacology; D058825:Serotonin 5-HT1 Receptor Agonists / Q000494:pharmacology; D058829:Serotonin 5-HT1 Receptor Antagonists / Q000494:pharmacology
chemical_list: C097067:CP 94253; D000697:Central Nervous System Stimulants; D010069:Oxadiazoles; D010879:Piperazines; D011725:Pyridines; D044302:Receptor, Serotonin, 5-HT1B; D044346:Receptor, Serotonin, 5-HT1D; D044402:Receptor, Serotonin, 5-HT2A; D044403:Receptor, Serotonin, 5-HT2B; D044405:Receptor, Serotonin, 5-HT2C; D058825:Serotonin 5-HT1 Receptor Agonists; D058829:Serotonin 5-HT1 Receptor Antagonists; C042390:alpha-methylserotonin; C090701:GR 127935; D012701:Serotonin; D006246:Harmaline; D002928:Cinanserin
keywords: Harmaline; Memory; Mice; Passive avoidance; Serotonergic system