title: Human dermal CD14⁺ cells are a transient population of monocyte-derived macrophages.
abstract: Dendritic cells (DCs), monocytes, and macrophages are leukocytes with critical roles in immunity and tolerance. The DC network is evolutionarily conserved; the homologs of human tissue CD141(hi)XCR1⁺ CLEC9A⁺ DCs and CD1c⁺ DCs are murine CD103⁺ DCs and CD64⁻ CD11b⁺ DCs. In addition, human tissues also contain CD14⁺ cells, currently designated as DCs, with an as-yet unknown murine counterpart. Here we have demonstrated that human dermal CD14⁺ cells are a tissue-resident population of monocyte-derived macrophages with a short half-life of <6 days. The decline and reconstitution kinetics of human blood CD14⁺ monocytes and dermal CD14⁺ cells in vivo supported their precursor-progeny relationship. The murine homologs of human dermal CD14⁺ cells are CD11b⁺ CD64⁺ monocyte-derived macrophages. Human and mouse monocytes and macrophages were defined by highly conserved gene transcripts, which were distinct from DCs. The demonstration of monocyte-derived macrophages in the steady state in human tissue supports a conserved organization of human and mouse mononuclear phagocyte system.
authors.affiliation: Institute of Cellular Medicine, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK; Singapore Immunology Network, Agency for Science Technology and Research (A-Star), 8A Biomedical Grove, Immunos, Singapore 138648.
authors.forename: Naomi
authors.initials: N
authors.lastname: McGovern
authors.affiliation: Singapore Immunology Network, Agency for Science Technology and Research (A-Star), 8A Biomedical Grove, Immunos, Singapore 138648.
authors.forename: Andreas
authors.initials: A
authors.lastname: Schlitzer
authors.affiliation: Institute of Cellular Medicine, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: Merry
authors.initials: M
authors.lastname: Gunawan
authors.affiliation: Institute of Cellular Medicine, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: Laura
authors.initials: L
authors.lastname: Jardine
authors.affiliation: Singapore Immunology Network, Agency for Science Technology and Research (A-Star), 8A Biomedical Grove, Immunos, Singapore 138648.
authors.forename: Amanda
authors.initials: A
authors.lastname: Shin
authors.affiliation: Institute of Cellular Medicine, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Poyner
authors.affiliation: Institute of Cellular Medicine, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: Kile
authors.initials: K
authors.lastname: Green
authors.affiliation: Institute of Cellular Medicine, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: Rachel
authors.initials: R
authors.lastname: Dickinson
authors.affiliation: Institute of Cellular Medicine, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: Xiao-Nong
authors.initials: XN
authors.lastname: Wang
authors.affiliation: Singapore Immunology Network, Agency for Science Technology and Research (A-Star), 8A Biomedical Grove, Immunos, Singapore 138648.
authors.forename: Donovan
authors.initials: D
authors.lastname: Low
authors.affiliation: Institute of Cellular Medicine, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: Katie
authors.initials: K
authors.lastname: Best
authors.affiliation: Institute of Cellular Medicine, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: Samuel
authors.initials: S
authors.lastname: Covins
authors.affiliation: Institute of Cellular Medicine, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Milne
authors.affiliation: Institute of Cellular Medicine, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: Sarah
authors.initials: S
authors.lastname: Pagan
authors.affiliation: Institute of Cellular Medicine, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: Khadija
authors.initials: K
authors.lastname: Aljefri
authors.affiliation: Institute of Cellular Medicine, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: Martin
authors.initials: M
authors.lastname: Windebank
authors.affiliation: Institute of Cellular Medicine, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: Diego
authors.initials: D
authors.lastname: Miranda-Saavedra
authors.affiliation: Singapore Immunology Network, Agency for Science Technology and Research (A-Star), 8A Biomedical Grove, Immunos, Singapore 138648.
authors.forename: Anis
authors.initials: A
authors.lastname: Larbi
authors.affiliation: Institute of Cellular Medicine, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: Pavandip Singh
authors.initials: PS
authors.lastname: Wasan
authors.affiliation: Singapore Immunology Network, Agency for Science Technology and Research (A-Star), 8A Biomedical Grove, Immunos, Singapore 138648.
authors.forename: Kaibo
authors.initials: K
authors.lastname: Duan
authors.affiliation: Singapore Immunology Network, Agency for Science Technology and Research (A-Star), 8A Biomedical Grove, Immunos, Singapore 138648; Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543.
authors.forename: Michael
authors.initials: M
authors.lastname: Poidinger
authors.affiliation: Institute of Cellular Medicine, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
authors.forename: Venetia
authors.initials: V
authors.lastname: Bigley
authors.affiliation: Singapore Immunology Network, Agency for Science Technology and Research (A-Star), 8A Biomedical Grove, Immunos, Singapore 138648.
authors.forename: Florent
authors.initials: F
authors.lastname: Ginhoux
authors.affiliation: Institute of Cellular Medicine, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK. Electronic address: matthew.collin@newcastle.ac.uk.
authors.forename: Matthew
authors.initials: M
authors.lastname: Collin
authors.affiliation: Institute of Cellular Medicine, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK. Electronic address: m.a.haniffa@newcastle.ac.uk.
authors.forename: Muzlifah
authors.initials: M
authors.lastname: Haniffa
mesh_terms: D000818:Animals; D039481:CD11b Antigen / Q000096:biosynthesis; D002454:Cell Differentiation / Q000276:immunology; D019070:Cell Lineage / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology; D005260:Female; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D017452:Receptors, IgG / Q000096:biosynthesis; D012867:Skin / Q000166:cytology / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D039481:CD11b Antigen; C509295:ITGAM protein, human; D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG