title: Cloning, expression, purification, crystallization and preliminary crystallographic analysis of the C-terminal domain of Par-4 (PAWR).
abstract: Prostate apoptosis response-4 protein is an intrinsically disordered pro-apoptotic protein with tumour suppressor function. Par-4 is known for its selective induction of apoptosis in cancer cells only and its ability to interact with various apoptotic proteins via its C-terminus. Par-4, with its unique function and various interacting partners, has gained importance as a potential target for cancer therapy. The C-terminus of the rat homologue of Par-4 was crystallized and a 3.7 Å resolution X-ray diffraction data set was collected. Preliminary data analysis shows the space group to be P41212. The unit-cell parameters are a = b = 115.351, c = 123.663 Å, α = β = γ = 90°.
authors.affiliation: Centre for Structural Systems Biology (CSSB), DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Udaya Kumar
authors.initials: UK
authors.lastname: Tiruttani Subhramanyam
authors.affiliation: Centre for Structural Systems Biology (CSSB), DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Kubicek
authors.affiliation: Centre for Structural Systems Biology (CSSB), DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Ulf B
authors.initials: UB
authors.lastname: Eidhoff
authors.affiliation: Centre for Structural Systems Biology (CSSB), DESY, Notkestrasse 85, 22607 Hamburg, Germany.
authors.forename: Joerg
authors.initials: J
authors.lastname: Labahn
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray
chemical_list: D051017:Apoptosis Regulatory Proteins; C101296:prostate apoptosis response-4 protein
keywords: Par-4; coiled coil; leucine zipper