title: Facile preparation of polyvinyl alcohol coated SiO2 stationary phases for high performance liquid chromatography.
abstract: A facile method to prepare a polar stationary phase for hydrophilic interaction chromatography (HILIC) was proposed by coating polyvinyl alcohol onto silica particles (PVA-Sil). A water or organic solvent-insoluble permanent PVA coating on the silica particle surface can be formed simply by dipping silica particles into a hot PVA solution and then settled from this solution, leaving a thin layer of PVA coating and frozen in a freezer. The PVA-Sil shields the silica core from solution erosion to some degree and the pH tolerance range was extended to 9.5 from 8.0 for bare silica. PVA-Sil demonstrated a good hydrophilic property for several kinds of polar compounds and ∼57000 m(-1) of plate count was achieved. This method can also be extended as a universal method to prepare various stationary phases with exchangeable functionalities by doping the desired ingredient in a PVA solution.
authors.affiliation: Pharmacy School, East-China Univ. Sci. Tech., 130 Meilong RD, Shanghai 200237, China. zhangff@ecust.edu.cn bcyang@ecust.edu.cn.
authors.forename: Shunli
authors.initials: S
authors.lastname: Ji
authors.forename: Feifang
authors.initials: F
authors.lastname: Zhang
authors.forename: Shengjie
authors.initials: S
authors.lastname: Wu
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Yang
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D012822:Silicon Dioxide; D011142:Polyvinyl Alcohol