title: Isolation and characterization of microsatellite loci in the invasive herb Solidago altissima (Asteraceae).
abstract: OBJECTIVE
  Microsatellite markers were developed in the perennial herb Solidago altissima from populations within its introduced range in Japan to assess its population structure and to facilitate tracking of invasion expansion. •
  
  
  RESULTS
  Using 454 pyrosequencing, 16 microsatellite primer sets were developed for S. altissima. The primer sets were tested on 70 individuals sampled from three populations in Japan. The primers amplified di- and trinucleotide repeats with five to 25 alleles per locus, and the expected heterozygosity ranged from 0.46 to 0.92. •
  
  
  CONCLUSIONS
  These results indicate the utility of primers in S. altissima for future research on a wide range of applications, including tracking of invasion dynamics and investigating population genetics of the species.
authors.affiliation: Laboratory of Forest Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Yuzu
authors.initials: Y
authors.lastname: Sakata
authors.affiliation: Faculty of Symbiotic Systems Science, Fukushima University, Fukushima 960-1296, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Kaneko
authors.affiliation: Wildlife Research Center, Kyoto University, Kyoto 606-8203, Japan.
authors.forename: Azusa
authors.initials: A
authors.lastname: Hayano
authors.affiliation: Wildlife Research Center, Kyoto University, Kyoto 606-8203, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Inoue-Murayama
authors.affiliation: Center for Ecological Research, Kyoto University, Otsu 520-2113, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ohgushi
authors.affiliation: Laboratory of Forest Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Isagi
keywords: 454 sequencing; Asteraceae; Solidago altissima; invasion; polyploidy