title: Mutational analysis of the genes encoding RFamide-related peptide-3, the human orthologue of gonadotrophin-inhibitory hormone, and its receptor (GPR147) in patients with gonadotrophin-releasing hormone-dependent pubertal disorders.
abstract: RFamide-related peptide-3 (RFRP-3), the orthologue of avian gonadotrophin-inhibitory hormone, and its receptor GPR147 have been recently identified in the human hypothalamus, and their roles in the regulation of reproductive axis has been studied. The present study aimed to investigate whether the presence of variants in the genes encoding human RFRP-3 (NPVF gene) and its receptor, GPR147 (NPFFR1 gene), is associated with the occurrence of gonadotrophin-releasing hormone-dependent pubertal disorders. Seventy-eight patients with idiopathic central precocious puberty (CPP) and 51 with normosmic isolated hypogonadotrophic hypogonadism (nIHH) were investigated. Fifty healthy subjects comprised the control group. The coding sequences of the NPVF and NPFFR1 genes were amplified and sequenced. Odds ratios (OR) were used to estimate the likelihood of CPP or nIHH in the presence of the described polymorphisms. All such polymorphisms have already been registered in the National Center for Biotechnology Information database. A three-nucleotide in frame deletion was identified in the NPVF gene (p.I71_K72), with a smaller proportion in the CPP (5%) compared to the nIHH (15%) group (P = 0.06). This results in the deletion of the isoleucine at position 71, adjacent to lysine at an endoproteolytic cleavage site of the precursor peptide. This polymorphism was associated with a lower risk of CPP (OR = 0.33; 95% confidence interval = 0.08-0.88); interestingly, only two men with nIHH were homozygotes for this variant. A total of five missense polymorphisms were found in the NPFFR1 gene, which encodes GPR147, with similar frequencies among groups and no association with pubertal timing. Our data suggest that RFRP-3/GPR147 may play secondary, modulatory roles on the regulation of pubertal development; a restraining modulatory effect of the NPVF p.I71_K72 variant on the activation of the gonadotrophic axis cannot be ruled out and deserves further investigation.
authors.affiliation: Laboratório de Farmacologia Molecular, Faculdade de Ciências da Saúde e Ambulatório de Endocrinologia das Gônadas e Adrenais, Hospital Universitário de Brasília, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: C J G
authors.initials: CJ
authors.lastname: Lima
authors.forename: S C
authors.initials: SC
authors.lastname: Cardoso
authors.forename: E F L
authors.initials: EF
authors.lastname: Lemos
authors.forename: E
authors.initials: E
authors.lastname: Zingler
authors.forename: C
authors.initials: C
authors.lastname: Capanema
authors.forename: L D
authors.initials: LD
authors.lastname: Menezes
authors.forename: G
authors.initials: G
authors.lastname: Vogado
authors.forename: B T A
authors.initials: BT
authors.lastname: Dos Santos
authors.forename: O L
authors.initials: OL
authors.lastname: de Moraes
authors.forename: E F
authors.initials: EF
authors.lastname: Duarte
authors.forename: V N
authors.initials: VN
authors.lastname: de Brito
authors.forename: A C
authors.initials: AC
authors.lastname: Latronico
authors.forename: A
authors.initials: A
authors.lastname: Lofrano-Porto
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D004252:DNA Mutational Analysis; D005260:Female; D006801:Humans; D007006:Hypogonadism / Q000235:genetics* / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D009479:Neuropeptides / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D011629:Puberty, Precocious / Q000235:genetics* / Q000378:metabolism; D018013:Receptors, Neuropeptide / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult
chemical_list: D009479:Neuropeptides; C416987:RFamide peptide; D018013:Receptors, Neuropeptide; C083929:neuropeptide FF receptor
keywords: GPR147; GnIH; GnRH; RFamide-related peptides; hypogonadotrophic hypogonadism; precocious puberty