title: Expression profile of Nanos2 gene in dairy goat and its inhibitory effect on Stra8 during meiosis.
abstract: OBJECTIVE
  Nanos2, an RNA-binding protein, belongs to the Nanos gene-coding family and contains two CCHC zinc-finger motifs. In mouse, it plays a pivotal role in male germ cell development, and self-renewal of spermatogonial stem cells. However, little is known of its expression pattern and functions in dairy goat testis.
  
  
  METHODS
  Immunohistochemistry and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) were used to generate the expression profile of Nanos2 in dairy goat testis. Furthermore, its overexpression effects on male germline stem cells (mGSCs) were studied using qRT-PCR, immunofluorescence, dual-luciferase reporter assay and western blotting.
  
  
  RESULTS
  Nanos2 is a conservative gene expressed widely in various tissues, especially in pancreas, and it displays higher expression in adult testes than in other age groups. Overexpression of Nanos2 significantly downregulated meiosis-related genes, including Stra8 and Scp3, which induced inhibition of meiosis. Results from dual-luciferase reporter assay and western blotting indicated that Nanos2 directly downregulated Stra8 in goat GmGSCs.
  
  
  CONCLUSIONS
  Taken together, these results suggest that Nanos2 plays an important role in spermatogonia and that its overexpression restrained meiosis in the dairy goat.
authors.affiliation: College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling, 712100, China; Shaanxi Stem Cell Engineering and Technology Research Center, Northwest Agriculture and Forestry University, Yangling, 712100, China.
authors.forename: X
authors.initials: X
authors.lastname: Yao
authors.forename: F
authors.initials: F
authors.lastname: Tang
authors.forename: M
authors.initials: M
authors.lastname: Yu
authors.forename: H
authors.initials: H
authors.lastname: Zhu
authors.forename: Z
authors.initials: Z
authors.lastname: Chu
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: W
authors.initials: W
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Hua
authors.forename: S
authors.initials: S
authors.lastname: Peng
mesh_terms: D053687:Adult Stem Cells / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D015870:Gene Expression; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D005822:Genetic Vectors; D005854:Germ Cells / Q000378:metabolism; D006041:Goats; D049452:Green Fluorescent Proteins / Q000235:genetics; D008297:Male; D008540:Meiosis / Q000235:genetics; D008969:Molecular Sequence Data; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis / Q000235:genetics; D010179:Pancreas / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D013093:Spermatogonia / Q000378:metabolism; D013573:Synaptonemal Complex / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014162:Transfection
chemical_list: D016601:RNA-Binding Proteins; D049452:Green Fluorescent Proteins; D052247:Nitric Oxide Synthase Type II