title: STV11 encodes a sulphotransferase and confers durable resistance to rice stripe virus.
abstract: Rice stripe virus (RSV) causes one of the most serious viral diseases of rice (Oryza sativa L.), but the molecular basis of RSV resistance has remained elusive. Here we show that the resistant allele of rice STV11 (STV11-R) encodes a sulfotransferase (OsSOT1) catalysing the conversion of salicylic acid (SA) into sulphonated SA (SSA), whereas the gene product encoded by the susceptible allele STV11-S loses this activity. Sequence analyses suggest that the STV11-R and STV11-S alleles were predifferentiated in different geographic populations of wild rice, Oryza rufipogon, and remained prevalent in cultivated indica and japonica rice varieties, respectively. Introgression of the STV11-R allele into susceptible cultivars or heterologous transfer of STV11-R into tobacco plants confers effective resistance against RSV. Our results shed new insights into plant viral defense mechanisms and suggest effective means of breeding RSV-resistant crops using molecular marker-assisted selection or genetic engineering.
authors.affiliation: 1] National key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China [2].
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: 1] National key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China [2].
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: National key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zheng
authors.affiliation: National key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Hu
authors.affiliation: National key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Liu
authors.affiliation: National key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Dai
authors.affiliation: National key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Baoxiang
authors.initials: B
authors.lastname: Wang
authors.affiliation: National key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Weixun
authors.initials: W
authors.lastname: Wu
authors.affiliation: National key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: He
authors.initials: H
authors.lastname: Gao
authors.affiliation: National key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Yunhui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Tao
authors.affiliation: National Hybrid Rice R&D Center, Changsha 410125, China.
authors.forename: Huafeng
authors.initials: H
authors.lastname: Deng
authors.affiliation: National Hybrid Rice R&D Center, Changsha 410125, China.
authors.forename: Dingyang
authors.initials: D
authors.lastname: Yuan
authors.affiliation: National key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Jiang
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Xiuping
authors.initials: X
authors.lastname: Guo
authors.affiliation: National key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xianian
authors.initials: X
authors.lastname: Cheng
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Chuanyin
authors.initials: C
authors.lastname: Wu
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Wang
authors.affiliation: National Hybrid Rice R&D Center, Changsha 410125, China.
authors.forename: Longping
authors.initials: L
authors.lastname: Yuan
authors.affiliation: 1] National key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China [2] National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Wan
mesh_terms: D000483:Alleles; D001947:Breeding; D018556:Crops, Agricultural / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D005818:Genetic Engineering; D012275:Oryza / Q000235:genetics* / Q000821:virology; D010935:Plant Diseases / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D020156:Salicylic Acid / Q000378:metabolism; D015238:Sulfotransferases / Q000235:genetics* / Q000378:metabolism; D029364:Tenuivirus; D014777:Virus Diseases / Q000235:genetics
chemical_list: D010940:Plant Proteins; D015238:Sulfotransferases; D020156:Salicylic Acid