title: Healing efficiency of oligosaccharides generated from almond gum (Prunus amygdalus) on dermal wounds of adult rats.
abstract: Almond gum is a naturally occurring polymer produced by almond trees and shrubs. Its abundance, as well as its low cost production makes it a potential feedstock for use in food and pharmaceuticals. In this regard, almond gum oligosaccharides were enzymatically generated, purified and their monosaccharide composition assessed using gas chromatography-flame ionization detector. Oligosaccharide analyses show that the most prominent residues were galactose and arabinose with traces of xylose, rhamnose, glucose and mannose. The glycosyl linkage positions were analyzed using gas chromatography - mass spectrometry showing a main chain composed of galactose units [→3)-Gal-(1→] branched mainly with arabinose residues [Ara-(1→]. The potent role of the generated oligosaccharides on rats wound healing was investigated. They have been applied either alone or supplemented, as active substance, with cream formulation, on full-thickness wound created on the dorsum of the rats. The effect of oligosaccharides was assessed by measuring the wound closure percentage, reaching an average of around 100% when applied alone or supplemented to cream formulation. The healing percentage for the control group was only 74.3% at the same day. The histological evaluation of skin sections visualized by light microscopy revealed an improved collagen deposition and an increased fibroblast and vascular densities.
authors.affiliation: Enzyme Bioconversion Unit (04/UR/09-04), National School of Engineering, P.O. Box 1173-3038, Sfax University, Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Bouaziz
authors.affiliation: Enzyme Bioconversion Unit (04/UR/09-04), National School of Engineering, P.O. Box 1173-3038, Sfax University, Tunisia.
authors.forename: Molka
authors.initials: M
authors.lastname: Ben Romdhane
authors.affiliation: Centre de Recherches sur les Macromolécules Végétales, C.N.R.S., Université Joseph Fourier, BP 53, Grenoble Cedex 9 38041, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Boisset Helbert
authors.affiliation: Centre de Recherches sur les Macromolécules Végétales, C.N.R.S., Université Joseph Fourier, BP 53, Grenoble Cedex 9 38041, France.
authors.forename: Laurine
authors.initials: L
authors.lastname: Buon
authors.affiliation: Enzyme Bioconversion Unit (04/UR/09-04), National School of Engineering, P.O. Box 1173-3038, Sfax University, Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Bhiri
authors.affiliation: Pharmacology Laboratory (UR 15/04), Sfax Medicine Faculty, 3029, Sfax University, Tunisia.
authors.forename: Sana
authors.initials: S
authors.lastname: Bardaa
authors.affiliation: Enzyme Bioconversion Unit (04/UR/09-04), National School of Engineering, P.O. Box 1173-3038, Sfax University, Tunisia.
authors.forename: Dorra
authors.initials: D
authors.lastname: Driss
authors.affiliation: Enzyme Bioconversion Unit (04/UR/09-04), National School of Engineering, P.O. Box 1173-3038, Sfax University, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Koubaa
authors.affiliation: Enzyme Bioconversion Unit (04/UR/09-04), National School of Engineering, P.O. Box 1173-3038, Sfax University, Tunisia.
authors.forename: Akram
authors.initials: A
authors.lastname: Fakhfakh
authors.affiliation: Pharmacology Laboratory (UR 15/04), Sfax Medicine Faculty, 3029, Sfax University, Tunisia.
authors.forename: Zouhair
authors.initials: Z
authors.lastname: Sahnoun
authors.affiliation: Enzyme Bioconversion Unit (04/UR/09-04), National School of Engineering, P.O. Box 1173-3038, Sfax University, Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Kallel
authors.affiliation: Animal Physiology Laboratory (UR 11/ES-70), Sfax Science Faculty, P.O. Box 1171-3000, Sfax University, Tunisia.
authors.forename: Najiba
authors.initials: N
authors.lastname: Zghal
authors.affiliation: Enzyme Bioconversion Unit (04/UR/09-04), National School of Engineering, P.O. Box 1173-3038, Sfax University, Tunisia; Common Service Unit of Bioreactor Coupled with an Ultrafilter, National School of Engineering, P.O. Box 1173-3038, Sfax University, Tunisia. Electronic address: semia.chaabouni@enis.rnu.tn.
authors.forename: Semia
authors.initials: S
authors.lastname: Ellouz Chaabouni
mesh_terms: D000375:Aging; D000818:Animals; D002845:Chromatography; D009844:Oligosaccharides / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000068181:Prunus dulcis / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000293:injuries*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D009844:Oligosaccharides
keywords: Almond gum; Arabinogalactan; Histopathology; Oligosaccharides; Wound healing