title: New insights into plant salt acclimation: the roles of vesicle trafficking and reactive oxygen species signalling in mitochondria and the endomembrane system.
abstract: In this study, we investigated the cellular and molecular mechanisms that regulate salt acclimation. The main objective was to obtain new insights into the molecular mechanisms that control salt acclimation. Therefore, we carried out a multidisciplinary study using proteomic, transcriptomic, subcellular and physiological techniques. We obtained a Nicotiana tabacum BY-2 cell line acclimated to be grown at 258 mM NaCl as a model for this study. The proteomic and transcriptomic data indicate that the molecular response to stress (chaperones, defence proteins, etc.) is highly induced in these salt-acclimated cells. The subcellular results show that salt induces sodium compartmentalization in the cell vacuoles and seems to be mediated by vesicle trafficking in tobacco salt-acclimated cells. Our results demonstrate that abscisic acid (ABA) and proline metabolism are crucial in the cellular signalling of salt acclimation, probably regulating reactive oxygen species (ROS) production in the mitochondria. ROS may act as a retrograde signal, regulating the cell response. The network of endoplasmic reticulum and Golgi apparatus is highly altered in salt-acclimated cells. The molecular and subcellular analysis suggests that the unfolded protein response is induced in salt-acclimated cells. Finally, we propose that this mechanism may mediate cell death in salt-acclimated cells.
authors.affiliation: Department of Abiotic Stress and Plant Pathology, CEBAS-CSIC, PO Box 164, Murcia, Spain.
authors.forename: Jesus
authors.initials: J
authors.lastname: Garcia de la Garma
authors.forename: Nieves
authors.initials: N
authors.lastname: Fernandez-Garcia
authors.forename: Enas
authors.initials: E
authors.lastname: Bardisi
authors.forename: Beatriz
authors.initials: B
authors.lastname: Pallol
authors.forename: Jose Salvador
authors.initials: JS
authors.lastname: Asensio-Rubio
authors.forename: Roque
authors.initials: R
authors.lastname: Bru
authors.forename: Enrique
authors.initials: E
authors.lastname: Olmos
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000064:Acclimatization / Q000187:drug effects*; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D005453:Fluorescence; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008315:Malondialdehyde / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D020543:Proteome / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D055049:Salt Tolerance; D015398:Signal Transduction / Q000187:drug effects*; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D059467:Transcriptome / Q000235:genetics; D022161:Transport Vesicles / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D010940:Plant Proteins; D020543:Proteome; D017382:Reactive Oxygen Species; D012965:Sodium Chloride; D008315:Malondialdehyde; D000040:Abscisic Acid; D011392:Proline; D012964:Sodium; D006861:Hydrogen Peroxide; D020169:Caspases; D005978:Glutathione
keywords: Nicotiana tabacum; endoplasmic reticulum; mitochondria; proline; reactive oxygen species (ROS); salt acclimation; vesicle trafficking