title: Oxidative damage to nucleic acids and benzo(a)pyrene-7,8-diol-9,10-epoxide-DNA adducts and chromosomal aberration in children with psoriasis repeatedly exposed to crude coal tar ointment and UV radiation.
abstract: The paper presents a prospective cohort study. Observed group was formed of children with plaque psoriasis (n=19) treated by Goeckerman therapy (GT). The study describes adverse (side) effects associated with application of GT (combined exposure of 3% crude coal tar ointment and UV radiation). After GT we found significantly increased markers of oxidative stress (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanosine, and 8-hydroxyguanine), significantly increased levels of benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE) DNA adducts (BPDE-DNA), and significantly increased levels of total number of chromosomal aberrations in peripheral lymphocytes. We found significant relationship between (1) time of UV exposure and total number of aberrated cells and (2) daily topical application of 3% crude coal tar ointment (% of body surface) and level of BPDE-DNA adducts. The findings indicated increased hazard of oxidative stress and genotoxic effects related to the treatment. However, it must be noted that the oxidized guanine species and BPDE-DNA adducts also reflect individual variations in metabolic enzyme activity (different extent of bioactivation of benzo[a]pyrene to BPDE) and overall efficiency of DNA/RNA repair system. The study confirmed good effectiveness of the GT (significantly decreased PASI score).
authors.affiliation: Institute of Pathological Physiology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, 50038 Hradec Kralove, Czech Republic.
authors.forename: Lenka
authors.identifier: 0000-0002-8580-1485
authors.initials: L
authors.lastname: Borska
authors.affiliation: Institute of Clinical Immunology and Allergology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Simkova 870, 50038 Hradec Kralove, Czech Republic.
authors.forename: Ctirad
authors.initials: C
authors.lastname: Andrys
authors.affiliation: Institute of Clinical Immunology and Allergology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Simkova 870, 50038 Hradec Kralove, Czech Republic.
authors.forename: Jan
authors.identifier: 0000-0003-2778-6800
authors.initials: J
authors.lastname: Krejsek
authors.affiliation: Institute of Clinical Biochemistry and Diagnosis, Faculty of Medicine in Hradec Kralove, Charles University in Prague, 50038 Hradec Kralove, Czech Republic.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Palicka
authors.affiliation: Institute of Clinical Biochemistry and Diagnosis, Faculty of Medicine in Hradec Kralove, Charles University in Prague, 50038 Hradec Kralove, Czech Republic.
authors.forename: Marcela
authors.initials: M
authors.lastname: Chmelarova
authors.affiliation: Clinic of Dermal and Venereal Diseases, University Hospital Hradec Kralove, Novy Hradec Kralove, 500 05 Hradec Kralove, Czech Republic.
authors.forename: Kvetoslava
authors.initials: K
authors.lastname: Hamakova
authors.affiliation: Institute of Pathological Physiology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, 50038 Hradec Kralove, Czech Republic.
authors.forename: Jan
authors.identifier: 0000-0001-8641-4287
authors.initials: J
authors.lastname: Kremlacek
authors.affiliation: Institute of Hygiene and Preventive Medicine, Charles University in Prague, Faculty of Medicine in Hradec Kralove, 50038 Hradec Kralove, Czech Republic.
authors.forename: Zdenek
authors.identifier: 0000-0001-9978-5757
authors.initials: Z
authors.lastname: Fiala
mesh_terms: D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000032:analysis / Q000737:chemistry*; D000293:Adolescent; D001564:Benzo(a)pyrene / Q000737:chemistry / Q000633:toxicity; D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations; D003033:Coal Tar / Q000494:pharmacology / Q000627:therapeutic use*; D015331:Cohort Studies; D004247:DNA / Q000737:chemistry*; D018736:DNA Adducts / Q000032:analysis / Q000737:chemistry*; D004249:DNA Damage / Q000187:drug effects; D005260:Female; D006801:Humans; D007641:Keratolytic Agents / Q000494:pharmacology / Q000627:therapeutic use; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D008297:Male; D009824:Ointments / Q000494:pharmacology / Q000627:therapeutic use; D018384:Oxidative Stress / Q000187:drug effects; D011446:Prospective Studies; D011565:Psoriasis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D014466:Ultraviolet Rays
chemical_list: D018736:DNA Adducts; D007641:Keratolytic Agents; D009824:Ointments; C043931:benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA; D001564:Benzo(a)pyrene; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D003033:Coal Tar; D004247:DNA