title: Crystallization of the C-terminal redox domain of the sulfur-assimilatory enzyme APR1 from Arabidopsis thaliana.
abstract: Plant-type APS reductase (APR), which catalyzes the reduction of activated sulfate to sulfite in plants, consists of a reductase domain and a C-terminal redox domain showing sequence homology to thioredoxin but possessing the activity of glutaredoxin. In order to understand the structural and biochemical properties of the redox domain of plant-type APS reductase, the C-terminal domain of APR1 (APR1C) from Arabidopsis thaliana was crystallized using the sitting-drop vapour-diffusion method. X-ray diffraction data were collected to a resolution of 2.70 Å on the SPXF beamline BL13B1 at the NSRRC, Taiwan. The crystals belonged to space group P43212 or P41212, with unit-cell parameters a = b = 58.2, c = 86.7 Å. With one molecule per asymmetric unit, the crystal volume per unit protein weight (VM) is 2.64 Å(3) Da(-1), which corresponds to a solvent content of approximately 53.49%. Further structure-based functional studies of APR1C would extend knowledge of the molecular mechanism and regulation of APR.
authors.affiliation: Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Chen
authors.affiliation: Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Yu-Yung
authors.initials: YY
authors.lastname: Chang
authors.affiliation: Genome and Systems Biology Degree Program, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Chao-Cheng
authors.initials: CC
authors.lastname: Cho
authors.affiliation: Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Hsu
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D013455:Sulfur / Q000737:chemistry*
chemical_list: D013455:Sulfur; D050862:Oxidoreductases Acting on Sulfur Group Donors; C019618:adenylylsulfate reductase
keywords: APR1; Arabidopsis thaliana; glutaredoxin; sulfonucleotide reductase; sulfur assimilation