title: Mannotriose regulates learning and memory signal transduction in the hippocampus.
abstract: Rehmannia is a commonly used Chinese herb, which improves learning and memory. However, the crucial components of the signal transduction pathway associated with this effect remain elusive. Pri-mary hippocampal neurons were cultured in vitro, insulted with high-concentration (1 × 10(-4) mol/L) cor-ticosterone, and treated with 1 × 10(-4) mol/L mannotriose. Thiazolyl blue tetrazolium bromide assay and western blot analysis showed that hippocampal neuron survival rates and protein levels of glucocorti-coid receptor, serum and glucocorticoid-regulated protein kinase, and brain-derived neurotrophic factor were all dramatically decreased after high-concentration corticosterone-induced injury. This effect was reversed by mannotriose, to a similar level as RU38486 and donepezil. Our findings indicate that mannotriose could protect hippocampal neurons from high-concentration corticosterone-induced injury. The mechanism by which this occurred was associated with levels of glucocorticoid receptor protein, serum and glucocorticoid-regulated protein kinase, and brain-derived neurotrophic factor.
authors.affiliation: Department of Biochemistry, Institute of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry, Institute of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Dai
authors.affiliation: Department of Biochemistry, Institute of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Xueli
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry, Institute of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Zhangbin
authors.initials: Z
authors.lastname: Gong
authors.affiliation: Department of Biochemistry, Institute of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Guoqin
authors.initials: G
authors.lastname: Jin
keywords: Rehmannia; corticosterone; grants-supported paper; hippocampus; learning and memory; mannotriose; neural regeneration; neurons; neuroregeneration; traditional Chinese medicine