title: Epstein-Barr virus encoded microRNAs target SUMO-regulated cellular functions.
abstract: Post-translational modification by the small ubiquitin-like modifier (SUMO) regulates the cellular response to different types of stress and plays a pivotal role in the control of oncogenic viral infections. Here we investigated the capacity of microRNAs (miRNAs) encoded by Epstein-Barr virus to interfere with the SUMO signaling network. Using a computational strategy that scores different properties of miRNA-mRNA target pairs, we identified a minimal set of 575 members of the SUMO interactome that may be targeted by one or more Epstein-Barr virus miRNAs. A significant proportion of the candidates cluster in a functional network that controls chromatin organization, stress, DNA damage and immune responses, apoptosis and transforming growth factor beta signaling. Multiple components of the transforming growth factor beta signaling pathway were inhibited upon upregulation of the BamHI-H rightward open reading frame 1 (BHRF1) encoded miRNAs in cells transduced with recombinant lentiviruses or entering the productive virus cycle. These findings point to the capacity of viral miRNAs to interfere with SUMO-regulated cellular functions that control key aspects of viral replication and pathogenesis.
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Simone
authors.initials: S
authors.lastname: Callegari
authors.forename: Stefano
authors.initials: S
authors.lastname: Gastaldello
authors.forename: Omid R
authors.initials: OR
authors.lastname: Faridani
authors.forename: Maria G
authors.initials: MG
authors.lastname: Masucci
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D017209:Apoptosis; D004249:DNA Damage; D015967:Gene Expression Regulation, Viral; D053263:Gene Regulatory Networks; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D035683:MicroRNAs / Q000494:pharmacology*; D016366:Open Reading Frames; D011499:Protein Processing, Post-Translational; D012313:RNA / Q000502:physiology; D012367:RNA, Viral / Q000494:pharmacology*; D015398:Signal Transduction; D025841:Small Ubiquitin-Related Modifier Proteins / Q000502:physiology*; D058207:Sumoylation; D014161:Transduction, Genetic; D016212:Transforming Growth Factor beta / Q000502:physiology; D014779:Virus Replication
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D012367:RNA, Viral; C039919:RNA, recombinant; D025841:Small Ubiquitin-Related Modifier Proteins; D016212:Transforming Growth Factor beta; D012313:RNA
keywords: EBV; SUMO; TGF-beta; miRNA; miRNA tartget prediction