title: Caries prevention and adhesiveness of restorative materials submitted to cariogenic mixed biofilm.
abstract: This in vitro study evaluated the mechanical properties of different restorative materials submitted to cariogenic conditions with induced mixed biofilm. Extracted bovine incisors (n = 108) were divided into 3 groups (n = 36) [Group 1, resin; Group 2, glass ionomer cement (GIC); and Group 3, resin-modified GIC] and were bonded on a previously prepared enamel surface with a 25 mm² area delimited with nail varnish. Each group was then further subdivided into 3 groups and tested for shear bond strength and effectiveness in caries protection. Groups 1A-3A were tested immediately after bonding, Groups 1B-3B were tested after 5 days in brain heart infusion media, and Groups 1C-3C were tested after 5 days under cariogenic conditions with mixed biofilm. The mixed biofilm system was composed of Streptococcus mutans and Candida albicans in order to artificially create white spot lesions (WSLs). Group 1 presented the most samples with WSLs, whereas Groups 2 and Group 3 presented the highest preventive effect (P < 0.05) across all subgroups. The mean bond strengths were highest in Group 1 across all subgroups (P < 0.05). The majority of the specimens in the Group 1 subgroups presented mixed and cohesive fractures, whereas Groups 2 and 3 subgroups presented the largest amount of adhesive fractures.
authors.forename: Thalita A
authors.initials: TA
authors.lastname: Miana
authors.forename: Tatiana Kelly da Silva
authors.initials: TK
authors.lastname: Fidalgo
authors.forename: Maristela Barbosa
authors.initials: MB
authors.lastname: Portela
authors.forename: Lucianne Cople
authors.initials: LC
authors.lastname: Maia
mesh_terms: D000268:Adhesiveness; D018441:Biofilms; D003731:Dental Caries / Q000517:prevention & control*; D006801:Humans
keywords: Candida albicans; Streptococcus mutans; dental caries; dental enamel; fluoride