title: Expression of DNA topoisomerase II-α: Clinical significance in laryngeal carcinoma.
abstract: DNA topoisomerase II-α (Topo II-α) is essential for numerous cell processes, including DNA replication, transcription, recombination, and chromosome separation and condensation. Altered Topo II-α expression may lead to carcinogenesis and cancer progression. The aim of the present study was to investigate the association between Topo II-α expression levels and clinicopathological data from laryngeal cancer patients. Immunohistochemistry was used to analyze Topo II-α expression in laryngeal squamous cell carcinoma and distant healthy tissues obtained from 70 patients. In addition, fluorescencein situhybridization was used to detect Topo II-α amplification and chromosome 17 ploidy using a laryngeal cancer tissue microarray. The expression of Topo II-α protein was detected in 71.43% (50/70) of laryngeal carcinoma tissues, in contrast to 9% of healthy tissues (2/22). Furthermore, the expression of Topo II-α protein was found to be associated with tumor de-differentiation and advanced tumor T stage. However, the expression of Topo II-α protein was not identified to be associated withTopo II-αamplification in laryngeal carcinoma, although was found to positively correlate with chromosome 17 aneuploidy (P<0.05). A higher aneuploidy rate contributed to increased expression levels of Topo II-α protein. Aberrant Topo II-α expression and chromosome 17 aneuploidy contributed to the development and progression of laryngeal cancer, indicating that targeting Topo II-α may provide a treatment strategy for patients with laryngeal cancer.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China ; Nursing College of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
authors.forename: Haili
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
authors.forename: Shuxin
authors.initials: S
authors.lastname: Wen
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Huangfu
authors.affiliation: Department of Pathology, Shanxi Cancer Hospital, Taiyuan, Shanxi 030013, P.R. China.
authors.forename: Ruifang
authors.initials: R
authors.lastname: Sun
authors.affiliation: Department of Pathology, Shanxi Cancer Hospital, Taiyuan, Shanxi 030013, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Bai
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China ; Nursing College of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
authors.forename: Binquan
authors.initials: B
authors.lastname: Wang
keywords: DNA topoisomerase II-α; chromosome 17 aneuploidy; fluorescence in situ hybridization; immunohistochemistry; laryngeal squamous cell carcinoma