title: Novel nanometer scaffolds regulate the biological behaviors of neural stem cells.
abstract: Ideal tissue-engineered scaffold materials regulate proliferation, apoptosis and differentiation of cells seeded on them by regulating gene expression. In this study, aligned and randomly oriented collagen nanofiber scaffolds were prepared using electronic spinning technology. Their diameters and appearance reached the standards of tissue-engineered nanometer scaffolds. The nanofiber scaffolds were characterized by a high swelling ratio, high porosity and good mechanical properties. The proliferation of spinal cord-derived neural stem cells on novel nanofiber scaffolds was obviously enhanced. The proportions of cells in the S and G2/M phases noticeably increased. Moreover, the proliferation rate of neural stem cells on the aligned collagen nanofiber scaffolds was high. The expression levels of cyclin D1 and cyclin-dependent kinase 2 were increased. Bcl-2 expression was significantly increased, but Bax and caspase-3 gene expressions were obviously decreased. There was no significant difference in the differentiation of neural stem cells into neurons on aligned and randomly oriented collagen nanofiber scaffolds. These results indicate that novel nanofiber scaffolds could promote the proliferation of spinal cord-derived neural stem cells and inhibit apoptosis without inducing differentiation. Nanofiber scaffolds regulate apoptosis and proliferation in neural stem cells by altering gene expression.
authors.affiliation: Longnan Hospital of Daqing, i.e. the Fifth Hospital Affiliated to Qiqihar Medical University, Daqing 163453, Heilongjiang Province, China.
authors.forename: Jihui
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Longnan Hospital of Daqing, i.e. the Fifth Hospital Affiliated to Qiqihar Medical University, Daqing 163453, Heilongjiang Province, China.
authors.forename: Fuge
authors.initials: F
authors.lastname: Sui
authors.affiliation: Department of Spine Surgery, the Second Hospital Affiliated to Harbin Medical University, Harbin 150086, Heilongjiang Province, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Yao
authors.affiliation: Department of Spine Surgery, the Second Hospital Affiliated to Harbin Medical University, Harbin 150086, Heilongjiang Province, China.
authors.forename: Yansong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Spine Surgery, the Second Hospital Affiliated to Harbin Medical University, Harbin 150086, Heilongjiang Province, China.
authors.forename: Yugang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Longnan Hospital of Daqing, i.e. the Fifth Hospital Affiliated to Qiqihar Medical University, Daqing 163453, Heilongjiang Province, China.
authors.forename: Feipeng
authors.initials: F
authors.lastname: Tian
authors.affiliation: Longnan Hospital of Daqing, i.e. the Fifth Hospital Affiliated to Qiqihar Medical University, Daqing 163453, Heilongjiang Province, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Longnan Hospital of Daqing, i.e. the Fifth Hospital Affiliated to Qiqihar Medical University, Daqing 163453, Heilongjiang Province, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: He
authors.affiliation: Longnan Hospital of Daqing, i.e. the Fifth Hospital Affiliated to Qiqihar Medical University, Daqing 163453, Heilongjiang Province, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Shao
authors.affiliation: Longnan Hospital of Daqing, i.e. the Fifth Hospital Affiliated to Qiqihar Medical University, Daqing 163453, Heilongjiang Province, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Liu
keywords: apoptosis; differentiation; nanofiber scaffolds; neural regeneration; neuroregeneration; proliferation; spinal cord-derived neural stem cells; stem cells; tissue engineering