title: Molecular processes studied at a single-molecule level using DNA origami nanostructures and atomic force microscopy.
abstract: DNA origami nanostructures allow for the arrangement of different functionalities such as proteins, specific DNA structures, nanoparticles, and various chemical modifications with unprecedented precision. The arranged functional entities can be visualized by atomic force microscopy (AFM) which enables the study of molecular processes at a single-molecular level. Examples comprise the investigation of chemical reactions, electron-induced bond breaking, enzymatic binding and cleavage events, and conformational transitions in DNA. In this paper, we provide an overview of the advances achieved in the field of single-molecule investigations by applying atomic force microscopy to functionalized DNA origami substrates.
authors.affiliation: Institute of Chemistry-Physical Chemistry, Universität Potsdam, Karl-Liebknecht-Straße 24-25, D-14476 Potsdam, Germany. bald@uni-potsdam.de.
authors.forename: Ilko
authors.initials: I
authors.lastname: Bald
authors.affiliation: Technical and Macromolecular Chemistry, University of Paderborn, Warburger Str. 100, D-33098 Paderborn, Germany. adrian.keller@uni-paderborn.de.
authors.forename: Adrian
authors.initials: A
authors.lastname: Keller
mesh_terms: D000818:Animals; D004247:DNA / Q000648:ultrastructure*; D054856:G-Quadruplexes; D006801:Humans; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000648:ultrastructure
chemical_list: D004247:DNA