title: A new species of Glugea Thélohan, 1891 in the red sea bream Pagrus major (Temminck & Schlegel) (Teleostei: Sparidae) from China.
abstract: A new microsporidian species is described from farmed red sea bream Pagrus major (Temminck & Schlegel) (Teleostei: Sparidae). Large numbers of spherical whitish xenomas were observed throughout the visceral organs of the host. Histological examination showed that the microsporidia caused several xenomas that were embedded in the intestinal muscularis externa or submucosa. Light and transmission electron microscopy examination of the spores also revealed morphological features typical of species of Glugea Thélohan, 1891. This microsporidian parasite has two different types of mature spores: microspores and macrospores. The spores are elongate-ovoid, with a large posterior vacuole. The polaroplast is bi-partite, with anterior and posterior parts comprising densely packed lamellae and loose membranes, respectively, and occupies approximately the anterior half of the spore. The polar filament is anisofilar, with 12-13 coils in a single layer almost touching the posterior spore wall. Comparison of the small subunit rDNA sequences revealed 92.7-98.1% identity with the sequences available from other Glugea spp. from piscine hosts. Phylogenetic analysis demonstrated that the microsporidian species studied clustered within the Glugea clade with strong support. Based on the differences in the morphological characteristics and molecular data, the microsporidian infecting P. major is considered to represent a species new to science, Glugea pagri n. sp.
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, Guangdong, China.
authors.forename: Youlu
authors.initials: Y
authors.lastname: Su
authors.forename: Juan
authors.initials: J
authors.lastname: Feng
authors.forename: Xiuxiu
authors.initials: X
authors.lastname: Sun
authors.forename: Jingzhe
authors.initials: J
authors.lastname: Jiang
authors.forename: Zhixun
authors.initials: Z
authors.lastname: Guo
authors.forename: Lingtong
authors.initials: L
authors.lastname: Ye
authors.forename: Liwen
authors.initials: L
authors.lastname: Xu
mesh_terms: D000818:Animals; D002681:China; D004275:DNA, Ribosomal / Q000235:genetics; D005393:Fish Diseases / Q000382:microbiology* / Q000473:pathology; D050220:Glugea / Q000145:classification* / Q000166:cytology / Q000235:genetics; D016881:Microsporidiosis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D008969:Molecular Sequence Data; D010802:Phylogeny; D021541:Sea Bream / Q000382:microbiology*; D013045:Species Specificity
chemical_list: D004275:DNA, Ribosomal