title: Antioxidant activity of yogurt made from milk characterized by different casein haplotypes and fortified with chestnut and sulla honeys.
abstract: The aim of this work was to evaluate the antioxidant activity of yogurt made from milk characterized by different casein (CN) haplotypes (αs1-, β-, κ-CN) and fortified with chestnut and sulla honeys. The CN haplotype was determined by isoelectric focusing, whereas antioxidant activity of yogurt was measured using 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid and ferric-reducing antioxidant power. The statistical analysis showed a significant effect of the studied factors. The results showed that chestnut honey presented the highest phenolic acid and flavonoid contents, which are closely associated with its high antioxidant activity. The antioxidant activity of fortified yogurt samples was affected both by different CN haplotypes and by type of honey added. Yogurts fortified with chestnut honey showed higher antioxidant activity than those fortified with sulla honey. The different behavior observed among the fortified yogurts led us to hypothesize that the effects of protein-polyphenol complex on antioxidant activity are interactive. The results suggest that milk proteins polymorphism and polyphenols play different roles in affecting the bioavailability and the antioxidant activity of yogurt.
authors.affiliation: School of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, Potenza, Viale dell'Ateneo Lucano 10 - 85100, Italy. Electronic address: anna.perna@unibas.it.
authors.forename: Annamaria
authors.initials: A
authors.lastname: Perna
authors.affiliation: School of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, Potenza, Viale dell'Ateneo Lucano 10 - 85100, Italy.
authors.forename: Immacolata
authors.initials: I
authors.lastname: Intaglietta
authors.affiliation: School of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, Potenza, Viale dell'Ateneo Lucano 10 - 85100, Italy.
authors.forename: Amalia
authors.initials: A
authors.lastname: Simonetti
authors.affiliation: School of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, Potenza, Viale dell'Ateneo Lucano 10 - 85100, Italy.
authors.forename: Emilio
authors.initials: E
authors.lastname: Gambacorta
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D002364:Caseins / Q000737:chemistry / Q000235:genetics; D004347:Drug Interactions; D007887:Fabaceae; D005419:Flavonoids; D005511:Food Handling / Q000379:methods*; D005527:Food, Fortified; D006239:Haplotypes; D006722:Honey; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis / Q000737:chemistry; D009754:Nuts; D010084:Oxidation-Reduction; D059808:Polyphenols / Q000737:chemistry; D015014:Yogurt / Q000032:analysis*
chemical_list: D000975:Antioxidants; D002364:Caseins; D005419:Flavonoids; D008894:Milk Proteins; D059808:Polyphenols
keywords: antioxidant activity; casein haplotype; honey; yogurt