title: Effects of hyperbaric oxygen therapy on the liver after injury caused by the hepatic ischemia-reperfusion process.
abstract: OBJECTIVE
  To evaluate the effects of hyperbaric oxygen on rats submitted to hepatic ischemia and reperfusion.
  
  
  METHODS
  Twenty-three Wistar rats were divided at random into 3 groups: SHAM, rats submitted to surgical and anesthetic stress without induction of hepatic ischemia/reperfurion; I/R, rats submitted to total ischemia of the hepatic pedicle for 25 min followed by 5 min of reperfusion; HBOI/R, rats submitted to 60 min of hyperbaric oxygen therapy at a pressure of 2 absolute atmospheres immediately after the experimental protocol of ischemia/reperfusion. Hepatic function was evaluated by quantitation of serum alanine aminotranferase (ALT) and aspartate aminotransferase (AST), and by mitochondrial function through the determination of states 3 and 4 of mitochondrial respiration, respiratory control ratio (RCR) and mitochondrial swelling. Data were analyzed by the Mann-Whitney test, with the level of significance set at p <0.05.
  
  
  RESULTS
  There was a significant difference in state 3 values for the SHAM group vs I/R and I/R vs IRHBO, in state 4 values for the SHAM group vs I/R; and in mitochondrial swelling for the SHAM groups vs I/RHBO, SHAM vs I/R, and IR vs I/RHBO.
  
  
  CONCLUSIONS
  The use of hyperbaric oxygen after I/R improved in a relative manner both the production of energy and the effects on the mitochondrial wall.
authors.affiliation: Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Marina Rodrigues Garcia da
authors.initials: MR
authors.lastname: Silveira
authors.affiliation: Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Maria Rita
authors.initials: MR
authors.lastname: Margarido
authors.affiliation: Ribeirão Preto Medical School, University of São Paulo, Brazil.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Vanni
authors.affiliation: Universidade Santo Amaro, São Paulo, SP, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Nejo Junior
authors.affiliation: Department of Surgery and Anatomy, FMRP, USP, Ribeirao Preto, SP, Brazil.
authors.forename: Orlando de
authors.initials: Od
authors.lastname: Castro-E-Silva
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D019069:Cell Respiration; D006931:Hyperbaric Oxygenation / Q000379:methods*; D008099:Liver / Q000098:blood supply*; D008297:Male; D008930:Mitochondria, Liver / Q000502:physiology; D011897:Random Allocation; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000628:therapy*; D015203:Reproducibility of Results; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase