title: Simultaneous quantification of eight bioactive secondary metabolites from Codonopsis ovata by validated high performance thin layer chromatography and their antioxidant profile.
abstract: Chemical investigation of Codonopsis ovata resulted in the isolation and identification of β-sitosterol-3-O-glycoside, luteolin, apigenin, gentiacaulein, swertiaperenine, β-sitosterol, taraxeryl-3-acetate, and 3β-acetoxyoleanane-12-one. A rapid, precise, sensitive and validated HPTLC method for simultaneous quantification of these natural products (NPs) was developed on silica-gel 60F254 plate using ternary solvent system, n-hexane:ethyl acetate:formic acid (10.5:3.5:0.43, v/v/v). Markers were quantified after post chromatographic derivatization with cerric ammonium sulfate reagent. The method was validated for accuracy, precision, LOD, LOQ and all calibration curves showed a good linear relationship (r>0.9924) within test range. Precision was evaluated by intra- and inter-day tests with RSDs <2.59%, accuracy validation recovery 92.43-99.50% with RSDs <1.00%. Apigenin was found major component (natural abundance: 1.103%) and β-sitosterol the least (0.0263%). The NPs displayed antioxidant activity with luteolin exhibiting maximum effect at 1μg/mL concentration (75.9% for DPPH and 43.7% for ABTS) and others at 10 and 25μg/mL, suggesting thereby their apparent potential use for the prevention of free radical induced diseases or as an additive element to food and pharmaceutical industry.
authors.affiliation: Bioorganic Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi 180001, India.
authors.forename: Alamgir A
authors.initials: AA
authors.lastname: Dar
authors.affiliation: Bioorganic Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi 180001, India. Electronic address: plsangwan@iiim.ac.in.
authors.forename: Payare L
authors.initials: PL
authors.lastname: Sangwan
authors.affiliation: Bioorganic Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi 180001, India.
authors.forename: Imran
authors.initials: I
authors.lastname: Khan
authors.affiliation: Bioorganic Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi 180001, India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Gupta
authors.affiliation: PK-PD Toxicology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi 180001, India.
authors.forename: Afnan
authors.initials: A
authors.lastname: Qaudri
authors.affiliation: PK-PD Toxicology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi 180001, India.
authors.forename: Sheikh A
authors.initials: SA
authors.lastname: Tasduq
authors.affiliation: Biodiversity and Applied Botany Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi 180001, India.
authors.forename: Surinder
authors.initials: S
authors.lastname: Kitchlu
authors.affiliation: School of Biology and Chemistry, Sri Mata Vaishno Devi University, Katra 182320, India.
authors.forename: Anil
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Bioorganic Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi 180001, India.
authors.forename: Surrinder
authors.initials: S
authors.lastname: Koul
mesh_terms: D000085:Acetates / Q000737:chemistry; D000645:Ammonium Sulfate / Q000737:chemistry; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002138:Calibration; D002855:Chromatography, Thin Layer / Q000379:methods*; D029745:Codonopsis / Q000378:metabolism*; D005561:Formates / Q000737:chemistry; D016166:Free Radical Scavengers / Q000032:analysis* / Q000494:pharmacology; D006586:Hexanes / Q000737:chemistry; D057230:Limit of Detection; D016014:Linear Models; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis* / Q000494:pharmacology; D010946:Plants, Medicinal; D012015:Reference Standards; D015203:Reproducibility of Results; D058428:Silica Gel / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D000085:Acetates; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005561:Formates; D016166:Free Radical Scavengers; D006586:Hexanes; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; D013451:Sulfonic Acids; C030544:formic acid; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C026385:n-hexane; D058428:Silica Gel; C007650:ethyl acetate; C004931:1,1-diphenyl-2-picrylhydrazyl; D000645:Ammonium Sulfate
keywords: Antioxidant; Codonopsis ovata; HPTLC; Quantification; Validation