title: [Identification of metabolites of antitumor lead compound T-OA in rat urine by HPLC-HRMS].
abstract: OBJECTIVE
  To study the major metabolites of antitumor lead compound T-OA (oleanolic acyl-3, 5, 6-trimethyl pyrazine-2-methyl ester) in rat urine, in order to preliminarily infer its metabolic mode in rats.
  
  
  METHODS
  Rat urines of the blank group, the raw material group (ligustrazine TMP and oleanolic acid OA Moore equivalent) and the T-OA group were collected and freeze-dried; Solids were extracted by ethyl acetate; And then the extracts were re-dissolved with acetonitrile. HPLC-HRMS coupling technique was adopted to find the potential mass spectrum peak under ESI(+) (see symbol) ESI(-) modes. Metabolite-related information was obtained by comparing the three groups of spectra.
  
  
  RESULTS
  One metabolite of OA and two metabolites of TMP were identified in the raw material group; none metabolite of T-OA but one phase II metabolite was detected in the T-OA group.
  
  
  CONCLUSIONS
  It is the first time to identify one phase II metabolite of T-OA and one phase II metabolite of OA were identified in rat urine. On that basis, the researchers preliminarily inferred that T-OA does not show the efficacy in the form of raw material. The HPLC-HRMS method established could be used to identify metabolites of related derivative structures. This paper could also provide certain reference for designing pro-drugs based on oleanolic acid.
authors.forename: Kuo
authors.initials: K
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Gong
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
authors.forename: Yu-Fei
authors.initials: YF
authors.lastname: Tian
authors.forename: Ming-Xi
authors.initials: MX
authors.lastname: Wang
authors.forename: Hua-Zheng
authors.initials: HZ
authors.lastname: Zhang
authors.forename: Yu-Zhong
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Hai-Min
authors.initials: HM
authors.lastname: Lei
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism* / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal