title: Effects of nutrients on matrix metalloproteinases in human T-lymphotropic virus type 1 positive and negative malignant T-lymphocytes.
abstract: Experimental and clinical studies have revealed the effectiveness of a specific nutrient synergy (SNS) mixture composed of ascorbic acid (AA), lysine, proline, arginine, epigallocatechin gallate (EGCG) and other micronutrients in targeting crucial physiological mechanisms involved in cancer progression and metastasis. HTLV-1 causes adult T-cell leukemia (ATL). The spread and metastases of ATL as well as other tumors has been associated with matrix metalloproteinases, especially the gelatinases MMP-2 and MMP-9. The objective of this study was to investigate whether SNS, AA and EGCG affects the gelatinolytic activity of MMP-2 and its transcriptional and translational levels in HTLV-1-positive and -negative malignant T-cells. The results indicated that SNS and EGCG caused a dose-dependent decline in the activity, transcription and translation of MMP-2 after treatment with SNS and EGCG, while AA was only able to inhibit the activity at maximum doses tested and to some extent, the protein expression levels of MMP-2, without affecting their transcriptional levels. The highest activity was noted in the case of SNS which is likely to be due to a synergistic effect of the different constituents in the formulation. These results point towards the potential integration of SNS in the anti-invasive treatment of ATL and related diseases.
authors.affiliation: Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Kingdom of Saudi Arabia.
authors.forename: Steve
authors.initials: S
authors.lastname: Harakeh
authors.affiliation: Molecular Tumor-genesis and Anticancer Pharmacology, EDST, Lebanese University, Hadath, Lebanon.
authors.forename: Raefa
authors.initials: R
authors.lastname: Abou-Khouzam
authors.affiliation: Department of Hematology, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
authors.forename: Ghazi A
authors.initials: GA
authors.lastname: Damanhouri
authors.affiliation: Department of Biological Sciences, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Al-Hejin
authors.affiliation: Department of Biochemistry, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
authors.forename: Taha
authors.initials: T
authors.lastname: Kumosani
authors.affiliation: Dr. Rath Research Institute, Santa Clara, CA, USA.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Niedzwiecki
authors.affiliation: Dr. Rath Research Institute, Santa Clara, CA, USA.
authors.forename: Mathias
authors.initials: M
authors.lastname: Rath
authors.affiliation: Department of Animal and Veterinary Sciences, American University of Beirut (AUB), Beirut 11-0236, Lebanon.
authors.forename: Elie
authors.initials: E
authors.lastname: Barbour
authors.affiliation: Molecular Tumor-genesis and Anticancer Pharmacology, EDST, Lebanese University, Hadath, Lebanon.
authors.forename: Mona
authors.initials: M
authors.lastname: Diab-Assaf
authors.affiliation: Molecular Tumor-genesis and Anticancer Pharmacology, EDST, Lebanese University, Hadath, Lebanon.
authors.forename: Rania
authors.initials: R
authors.lastname: Azar
mesh_terms: D001205:Ascorbic Acid / Q000008:administration & dosage*; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D004357:Drug Synergism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D015490:HTLV-I Infections / Q000188:drug therapy / Q000235:genetics / Q000473:pathology; D015368:Human T-lymphotropic virus 1 / Q000187:drug effects / Q000472:pathogenicity; D006801:Humans; D015458:Leukemia, T-Cell / Q000188:drug therapy* / Q000235:genetics / Q000821:virology; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis* / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D014176:Protein Biosynthesis / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D002392:Catechin; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; C057580:gallocatechol; D001205:Ascorbic Acid