title: Comparison of Coconut Water and Jordanian Propolis on Survival of Bench-dried Periodontal Ligament Cells: An in vitro Cell Culture Study.
abstract: OBJECTIVE
  The aim of this study is to assess and compare the efficacy of Jordanian propolis and full concentration mature coconut water in their ability to preserve periodontal ligament (PDL) cell viability after exposure of PDL cells to up to 120 minutes dry storage.
  
  
  METHODS
  PDL cells were obtained from sound permanent first molars which were cultured in Dulbecco's Modified Eagles Medium (DMEM). Cultures were subjected to 0, 30, 45, 60, 90 and 120 minutes dry storage times then incubated with 100% mature coconut water, Jordanian propolis and DMEM for 45 minutes at room temperature (18-26°C). Untreated cells served as controls at each dry storage time tested. PDL cell viability was assessed by MTT assay. Statistical analysis of data was accomplished by using one-way analysis of variance complemented by Tukey test and the level of significance was 5% ( p < 0.05).
  
  
  RESULTS
  Up to 60 minutes dry storage, no significant improvement on the percentage of viable cells was found from soaking in all tested media. On the other hand, soaking in mature coconut water only resulted in higher percentages of viable cells at >60 minutes dry storage. However, this improvement was not significant (p > 0.05).
  
  
  CONCLUSIONS
  Avulsed teeth which have been left dry for <45 minutes should be replanted immediately, whereas avulsed teeth which have been left dry for >45 minutes may benefit from soaking for 45 minutes in mature coconut water. How to cite this article: Al-Haj Ali SN, Al-Jundi S, Mhaidat N. Comparison of Coconut Water and Jordanian Propolis on Survival of Bench-dried Periodontal Ligament Cells: An in vitro Cell Culture Study. Int J Clin Pediatr Dent 2013;6(3):161-165.
authors.affiliation: Assistant Professor, Department of Orthodontics and Pediatric Dentistry, Qassim University, PO Box 6666, Qassim, Kingdom of Saudi Arabia, e-mail: dr.sanaa.alhajali@qudent.org.
authors.forename: Sanaa Najeh
authors.initials: SN
authors.lastname: Al-Haj Ali
authors.affiliation: Professor, Department of Pediatric and Preventive Dentistry, Jordan University of Science and Technology, Irbid, Jordan.
authors.forename: Suhad
authors.initials: S
authors.lastname: Al-Jundi
authors.affiliation: Associate Professor, Department of Clinical Pharmacy, Jordan University of Science and Technology, Irbid, Jordan.
authors.forename: Nizar
authors.initials: N
authors.lastname: Mhaidat
keywords: Avulsion; Cell culture; Dry storage; Mature coconut water; Propolis