title: Comparative cytotoxicity of artemisinin and cisplatin and their interactions with chlorogenic acids in MCF7 breast cancer cells.
abstract: In parts of Africa and Asia, self-medication with a hot water infusion of Artemisia annua (Artemisia tea) is a common practice for a number of ailments including malaria and cancer. In our earlier work, such an extract showed better potency than artemisinin alone against both chloroquine-sensitive and -resistant parasites. In this study, in vitro tests of the infusion in MCF7 cells showed high IC50 values (>200 μM). The combination of artemisinin and 3-caffeoylquinic acid (3CA), two major components in the extract, was strongly antagonistic and gave a near total loss of cytotoxicity for artemisinin. We observed that the interaction of 3CAs with another cytotoxic compound, cisplatin, showed potentiation of activity by 2.5-fold. The chelation of cellular iron by 3CA is hypothesized as a possible explanation for the loss of artemisinin activity.
authors.affiliation: Chemical Engineering and Biotechnology, University of Cambridge, CB2 3RA (UK).
authors.forename: John O
authors.initials: JO
authors.lastname: Suberu
authors.forename: Isolda
authors.initials: I
authors.lastname: Romero-Canelón
authors.forename: Neil
authors.initials: N
authors.lastname: Sullivan
authors.forename: Alexei A
authors.initials: AA
authors.lastname: Lapkin
authors.forename: Guy C
authors.initials: GC
authors.lastname: Barker
mesh_terms: D019306:Artemisia / Q000737:chemistry / Q000378:metabolism; D037621:Artemisinins / Q000737:chemistry* / Q000627:therapeutic use / Q000633:toxicity; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D002726:Chlorogenic Acid / Q000737:chemistry* / Q000627:therapeutic use / Q000633:toxicity; D002738:Chloroquine / Q000633:toxicity; D002945:Cisplatin / Q000737:chemistry* / Q000627:therapeutic use / Q000633:toxicity; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D004359:Drug Therapy, Combination; D005260:Female; D006801:Humans; D020128:Inhibitory Concentration 50; D061986:MCF-7 Cells; D010936:Plant Extracts / Q000737:chemistry
chemical_list: D037621:Artemisinins; D010936:Plant Extracts; D002726:Chlorogenic Acid; D002738:Chloroquine; C031327:artemisinin; D002945:Cisplatin
keywords: Artemisia tea; antagonism; artemisinin; chlorogenic acid; cisplatin; synergy