title: RNase Z(S1) processes UbL40 mRNAs and controls thermosensitive genic male sterility in rice.
abstract: Thermosensitive genic male-sterile (TGMS) lines, which are male-sterile at restrictive (high) temperatures but male-fertile at permissive (low) temperatures, have been widely used in breeding two-line hybrid rice (Oryza sativa L.). Here we find that mutation of thermosensitive genic male sterile 5 (tms5) in rice causes the TGMS trait through a loss of RNase Z(S1) function. We show that RNase Z(S1) processes the mRNAs of three ubiquitin fusion ribosomal protein L40 (UbL40) genes into multiple fragments in vitro and in vivo. In tms5 mutants, high temperature results in increased levels of UbL40 mRNAs. Overaccumulation of UbL40 mRNAs causes defective pollen production and male sterility. Our results uncover a novel mechanism of RNase Z(S1)-mediated UbL40 mRNA regulation and shows that loss of this regulation produces TGMS in rice, a finding with potential applications in hybrid crop breeding.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Zhou
authors.affiliation: 1] State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China [2] University of the Chinese Academy of Sciences, Yuquan Road, Beijing 100039, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.affiliation: 1] State Key Laboratory of Hybrid Rice, Changsha 410125, China [2] Yuan Longping High-tech Agriculture Co., Ltd., Changsha 410125, China [3] Yahua Seeds Science Academy of Hunan, Changsha 410119, China.
authors.forename: Yuanzhu
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: 1] State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China [2] Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
authors.forename: Liya
authors.initials: L
authors.lastname: Zhu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Dagang
authors.initials: D
authors.lastname: Jiang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Jingfang
authors.initials: J
authors.lastname: Dong
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Qinjian
authors.initials: Q
authors.lastname: Liu
authors.affiliation: 1] State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China [2] University of the Chinese Academy of Sciences, Yuquan Road, Beijing 100039, China.
authors.forename: Lianfeng
authors.initials: L
authors.lastname: Gu
authors.affiliation: 1] State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China [2] Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
authors.forename: Lingyan
authors.initials: L
authors.lastname: Zhou
authors.affiliation: 1] State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China [2] University of the Chinese Academy of Sciences, Yuquan Road, Beijing 100039, China.
authors.forename: Mingji
authors.initials: M
authors.lastname: Feng
authors.affiliation: Yahua Seeds Science Academy of Hunan, Changsha 410119, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Qin
authors.affiliation: Yahua Seeds Science Academy of Hunan, Changsha 410119, China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Hu
authors.affiliation: 1] University of the Chinese Academy of Sciences, Yuquan Road, Beijing 100039, China [2] State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Chengli
authors.initials: C
authors.lastname: Song
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xianwei
authors.initials: X
authors.lastname: Song
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Erdong
authors.initials: E
authors.lastname: Ni
authors.affiliation: State Key Laboratory of Hybrid Rice, Changsha 410125, China.
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Hybrid Rice, Changsha 410125, China.
authors.forename: Qiyun
authors.initials: Q
authors.lastname: Deng
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Zhenlan
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Yao-Guang
authors.initials: YG
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Cao
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Chuxiong
authors.initials: C
authors.lastname: Zhuang
mesh_terms: D004722:Endoribonucleases / Q000235:genetics* / Q000378:metabolism; D006358:Hot Temperature; D012275:Oryza / Q000235:genetics*; D051479:Plant Infertility / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011058:Pollen / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism; D013696:Temperature
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D004722:Endoribonucleases; C110250:RNase Z