title: Isolation of 16 microsatellite markers for Spiraea alpina and S. mongolica (Rosaceae) of the Qinghai-Tibet Plateau.
abstract: OBJECTIVE
  A set of microsatellite markers were developed to characterize the level of genetic diversity and gene flow in two plant species endemic to the Qinghai-Tibet Plateau, Spiraea alpina and S. mongolica. •
  
  
  RESULTS
  Using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) method, 16 microsatellite loci showed polymorphisms in both species. In two populations of each species, the number of alleles per locus ranged from three to 18 in S. alpina and from four to 30 in S. mongolica. •
  
  
  CONCLUSIONS
  These microsatellite markers provide an efficient tool for population genetic studies and will be used to assess the genetic diversity and spatial genetic structure of S. alpina and S. mongolica.
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, People's Republic of China ; University of the Chinese Academy of Sciences, Beijing 100039, People's Republic of China.
authors.forename: Gulzar
authors.initials: G
authors.lastname: Khan
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, People's Republic of China ; Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Faqi
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, People's Republic of China.
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, People's Republic of China.
authors.forename: Xiujie
authors.initials: X
authors.lastname: Jiao
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, People's Republic of China.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Fu
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xing
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, People's Republic of China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, People's Republic of China.
authors.forename: Shilong
authors.initials: S
authors.lastname: Chen
keywords: Qinghai–Tibet Plateau; Spiraea; gene flow; genetic diversity; microsatellite markers; population genetics