title: [Effect of verapamil and nitroglycerin on transplanted lung function in canines].
abstract: OBJECTIVE
  To investigate the protective effect of combined administration of verapamil and nitroglycerin on the function of canine transplanted lungs.
  
  
  METHODS
  Twenty orthotopic left lung transplantations were performed in 40 canines, which were randomly divided into 4 groups. In group I (control), the donor lungs were perfused and preserved with LPD solution, while group II with LPD solution plus verapamil 0.1 g/L, group III with LPD solution plus nitroglycerin 0.1g/L, and group IV with LPD solution plus verapamil 0.1 g/L and nitroglycerin 0.1 g/L. Hemodynamics and graft gas exchange were assessed 0, 2 and 4 h after the operation. The lung grafts were harvested to measure the wet/dry weight ratio, malondialdehyde (MDA) contents and superoxide dismutase (SOD) activity.
  
  
  RESULTS
  Compared with group I, II and III, the mean pulmonary artery pressure (MPAP), pulmonary vascular resistance index (PVRI), partial pressure of oxygen in arterial blood (PaO₂), dynamic compliance (Cdyn) and alveolar-arterial oxygen tension volume [P(A- a)O₂] in group IV were improved significantly (P<0.05). No significant difference in the partial pressure of carbondioxide (PaCO₂) and peak inspiratory pressure (PIP) was observed in the 4 groups (P>0.05). In group IV, the wet/dry weight ratio and MDA contents were lower than those in the other 3 groups, and the SOD activity was significantly higher than that of the other 3 groups (P<0.05).
  
  
  CONCLUSIONS
  Verapamil and nitroglycerin in LPD solution can protect the respiratory function of canine lung grafts by attenuating pulmonary edema and oxidative stress.
authors.affiliation: Department of Cardiothoracic Surgery, Third Xiangya Hospital, Central South University, Changsha 410013,China.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Jiang
authors.forename: Ping
authors.initials: P
authors.lastname: Hu
authors.forename: Jianxin
authors.initials: J
authors.lastname: Liu
authors.forename: Dianjun
authors.initials: D
authors.lastname: Wang
authors.forename: Longyu
authors.initials: L
authors.lastname: Jin
authors.forename: Chao
authors.initials: C
authors.lastname: Hong
mesh_terms: D000818:Animals; D001784:Blood Gas Analysis; D004285:Dogs; D006439:Hemodynamics; D008168:Lung; D016040:Lung Transplantation; D005996:Nitroglycerin / Q000494:pharmacology*; D009926:Organ Preservation; D020011:Protective Agents; D015427:Reperfusion Injury; D014700:Verapamil / Q000494:pharmacology*
chemical_list: D020011:Protective Agents; D014700:Verapamil; D005996:Nitroglycerin