title: Development and characterization of microsatellite loci in the endangered species Taxus wallichiana (Taxaceae).
abstract: OBJECTIVE
  Microsatellite primers were developed in the endangered tree species Taxus wallichiana from Nepal to investigate regional genetic differentiation, local genetic diversity, and gene flow for the conservation of this species under climate- and land-use change scenarios in mountain regions of Nepal. •
  
  
  RESULTS
  We developed 10 highly polymorphic microsatellite markers from 454 DNA sequencing. Characterization of the new microsatellite loci was done in 99 individuals collected from three valleys with different climatic regimes. The number of alleles per locus varied from four to 12. Observed heterozygosity of populations, averaged across loci, ranged from 0.30 to 0.59. •
  
  
  CONCLUSIONS
  The new markers provided by this study will substantially increase the resolution for detailed studies in phylogeography, population genetics, and parentage analysis.
authors.affiliation: Central Department of Botany, Tribhuvan University, Kathmandu, Nepal ; Swiss Federal Research Institute WSL, Zuercherstrasse 111, CH-8903 Birmensdorf, Switzerland.
authors.forename: Jyoti Prasad
authors.initials: JP
authors.lastname: Gajurel
authors.affiliation: Swiss Federal Research Institute WSL, Zuercherstrasse 111, CH-8903 Birmensdorf, Switzerland.
authors.forename: Carolina
authors.initials: C
authors.lastname: Cornejo
authors.affiliation: Swiss Federal Research Institute WSL, Zuercherstrasse 111, CH-8903 Birmensdorf, Switzerland.
authors.forename: Silke
authors.initials: S
authors.lastname: Werth
authors.affiliation: Central Department of Botany, Tribhuvan University, Kathmandu, Nepal.
authors.forename: Krishna Kumar
authors.initials: KK
authors.lastname: Shrestha
authors.affiliation: Swiss Federal Research Institute WSL, Zuercherstrasse 111, CH-8903 Birmensdorf, Switzerland.
authors.forename: Christoph
authors.initials: C
authors.lastname: Scheidegger
keywords: 454 pyrosequencing; Himalayan yew; Himalayas; conservation genetics; simple sequence repeat marker