title: Simultaneous determination of 30 hormones illegally added to anti-ageing functional foods using UPLC-MS/MS coupled with SPE clean-up.
abstract: A novel analytical method employing solid-phase extraction (SPE) coupled with ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed for the simultaneous determination of 30 hormones in anti-ageing functional foods (capsules, powders and tablets). The analytes were extracted with acetic acid-acetonitrile (1-99 v/v), methanol and acetone, respectively. The extract was purified using a combined column, followed by analyte detection with electrospray ionisation in positive- or negative-ion modes. The results indicated that the 30 compounds had good linear correlations in the range of 1-1000 μg kg⁻¹, and the correlation coefficients were above 0.99. The limits of detection (LOD) and limits of quantification (LOQ) were 0.03-2 and 0.1-5 μg kg⁻¹, respectively. The average recovery of 30 compounds at the three spiked levels varied from 74.7% to 124.1%, and the relative standard deviation (RSD) was 2.4-15.0%. This method was applied to the analysis of hormones in 14 real samples of which seven hormones (such as estrone, dienestrol) were detected in four samples, but the remainder of the hormones were not detected. The developed method is sensitive, efficient, reliable and applicable to real samples.
authors.affiliation: a College of Pharmacy , Chongqing Medical University , Yuzhong District , Chongqing , China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: He
authors.forename: Cunxian
authors.initials: C
authors.lastname: Xi
authors.forename: Bobin
authors.initials: B
authors.lastname: Tang
authors.forename: Guomin
authors.initials: G
authors.lastname: Wang
authors.forename: Dongdong
authors.initials: D
authors.lastname: Chen
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.forename: Zhaode
authors.initials: Z
authors.lastname: Mu
mesh_terms: D019588:Aging, Premature / Q000517:prevention & control; D053000:Analytic Sample Preparation Methods; D000975:Antioxidants / Q000737:chemistry* / Q000191:economics / Q000592:standards; D002681:China; D002851:Chromatography, High Pressure Liquid; D004028:Dienestrol / Q000032:analysis / Q000737:chemistry; D019587:Dietary Supplements / Q000032:analysis* / Q000191:economics / Q000592:standards; D004967:Estrogens / Q000032:analysis / Q000737:chemistry; D004968:Estrogens, Non-Steroidal / Q000032:analysis / Q000737:chemistry; D004970:Estrone / Q000032:analysis / Q000737:chemistry; D005506:Food Contamination; D005513:Food Inspection / Q000379:methods*; D019983:Guideline Adherence; D006728:Hormones / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D015394:Molecular Structure; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D004967:Estrogens; D004968:Estrogens, Non-Steroidal; D006728:Hormones; D004970:Estrone; D004028:Dienestrol
keywords: UPLC-MS/MS; anti-ageing functional food; hormone; solid-phase extraction