title: Whole genome shotgun sequences for microsatellite discovery and application in cultivated and wild Macadamia (Proteaceae).
abstract: OBJECTIVE
  Next-generation sequencing (NGS) data are widely used for single-nucleotide polymorphism discovery and genetic marker development in species with limited available genome information. We developed microsatellite primers for the Proteaceae nut crop species Macadamia integrifolia and assessed cross-species transferability in all congeners to investigate genetic identification of cultivars and gene flow. •
  
  
  RESULTS
  Primers were designed from both raw and assembled Illumina NGS paired-end reads. The final 12 microsatellite markers selected were polymorphic among wild individuals of all four Macadamia species-M. integrifolia, M. tetraphylla, M. ternifolia, and M. jansenii-and in commercial macadamia cultivars including hybrids. •
  
  
  CONCLUSIONS
  We demonstrate the utility of raw and assembled Illumina NGS reads from total genomic DNA for the rapid development of microsatellites in Macadamia. These primers will facilitate future studies of population structure, hybridization, parentage, and cultivar identification in cultivated and wild Macadamia populations.
authors.affiliation: Southern Cross Plant Science, Southern Cross University, Lismore, New South Wales, Australia.
authors.forename: Catherine J
authors.initials: CJ
authors.lastname: Nock
authors.affiliation: Southern Cross Plant Science, Southern Cross University, Lismore, New South Wales, Australia.
authors.forename: Martin S
authors.initials: MS
authors.lastname: Elphinstone
authors.affiliation: Southern Cross Plant Science, Southern Cross University, Lismore, New South Wales, Australia.
authors.forename: Gary
authors.initials: G
authors.lastname: Ablett
authors.affiliation: Southern Cross Plant Science, Southern Cross University, Lismore, New South Wales, Australia.
authors.forename: Asuka
authors.initials: A
authors.lastname: Kawamata
authors.affiliation: Southern Cross Plant Science, Southern Cross University, Lismore, New South Wales, Australia.
authors.forename: Wayne
authors.initials: W
authors.lastname: Hancock
authors.affiliation: Queensland Alliance for Agriculture and Food Innovation, University of Queensland, St. Lucia, Queensland, Australia.
authors.forename: Craig M
authors.initials: CM
authors.lastname: Hardner
authors.affiliation: Southern Cross Plant Science, Southern Cross University, Lismore, New South Wales, Australia.
authors.forename: Graham J
authors.initials: GJ
authors.lastname: King
keywords: Macadamia; Proteaceae; crop; cultivar; horticulture; nut