title: Development of a biological protocol for endotoxin detection using quartz crystal microbalance (QCM).
abstract: In this paper, a biological protocol for endotoxin detection has been developed and optimized by quartz crystal microbalance (QCM). The parameters involved in the formation of the self-assembled monolayer (SAM) have been analyzed, and a study of the pH of the ligand buffer has been performed in order to find the best condition for the ligand immobilization and, in consequence, for the endotoxin detection. The detection limit obtained with the characterized biological protocol corresponds to 1.90 μg/ml. The effectiveness of the optimized biological protocol has been analyzed by cyclic voltammetry analysis.
authors.affiliation: CEIT-IK4 and Tecnun, University of Navarra, Paseo de Manuel Lardizábal 15, 20.018, Donostia-San Sebastián, Spain, eperez@ceit.es.
authors.forename: E
authors.initials: E
authors.lastname: Pérez-Lorenzo
authors.forename: A
authors.initials: A
authors.lastname: Zuzuarregui
authors.forename: S
authors.initials: S
authors.lastname: Arana
authors.forename: M
authors.initials: M
authors.lastname: Mujika
mesh_terms: D001681:Biological Assay / Q000379:methods*; D004731:Endotoxins / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*
chemical_list: D004731:Endotoxins