title: Chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro.
abstract: This study investigated the effects of small interfering RNA (siRNA)-mediated silencing of chemokine receptor 4 (CXCR4) on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXCR4 mRNA and protein was significantly suppressed in transfected cells by all three sequence-specific siRNAs compared with control groups. Furthermore, the invasion capacity of SH-SY5Y cells was significantly decreased following transfection with CXCR4-specific siRNA compared with the control groups. These data demonstrate that down-regulation of CXCR4 can inhibit in vitro invasion of neuroblastoma.
authors.affiliation: Department of Pediatric Surgery, the Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Pediatric Surgery, the Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China.
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Operating Room, the Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Pediatric Surgery, the Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China.
authors.forename: Hongting
authors.initials: H
authors.lastname: Lu
authors.affiliation: Department of Pediatric Surgery, the Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China.
authors.forename: Xiwei
authors.initials: X
authors.lastname: Hao
authors.affiliation: Department of Pediatric Surgery, the Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Dong
keywords: NSFC grant; Transwell chamber; chemokine receptor 4; invasion; liposome; nerve regeneration; neural regeneration; neuroblastoma; small interfering RNA