title: Perfusion of a cerebral protective solution enhances neuroprotection in a rabbit model of occlusion-reperfusion: prolonged cerebral dormancy time.
abstract: In the present study, we investigated the effect of a cerebral protective solution on prolongation of cerebral dormancy time in a rabbit model of occlusion-reperfusion. In a control group, rabbits were anesthetized and the four cerebral arteries (the left and right common carotid arteries and vertebral arteries) were occluded for 7.5 min followed by reperfusion. All six rabbits in the control group died. In contrast, a second group underwent perfusion of a cerebral protective solution for 15 min between artery occlusion and reperfusion. All six rabbits in this group survived. However, when the perfusion solution was changed to 5% glucose solution or rabbit plasma in two other groups, the rabbits in both the latter two groups also died. Neuroprotection was also observed when the protective solution was administered for 30-60 min after the onset of artery occlusion and before the return of blood flow (reperfusion). To understand the high rate of thrombotic stroke in the clinic, we assessed the influence of different organ tissue infusions on blood coagulation in vitro and found that blood clotting occurred faster in the presence of brain tissue infusion compared to liver, kidney, and heart tissue infusions. These results indicate a higher rate of thrombosis in brain tissue compared to any of the other tissues tested. The current study shows that perfusion of a cerebral protective solution produced a significant neuroprotective benefit in our rabbit model of occlusion-reperfusion, suggesting that administration of a cerebral protective solution may be an effective approach for the treatment of ischemic stroke.
authors.affiliation: Department of Medicine, Rui Kang Hospital, Guangxi University of Chinese Medicine, Nanning, P.R. China liquenti@mail.nih.gov gxyelibin@sina.com.
authors.forename: Libin
authors.initials: L
authors.lastname: Ye
authors.affiliation: Department of Medicine, Rui Kang Hospital, Guangxi University of Chinese Medicine, Nanning, P.R. China.
authors.forename: Aiyuan
authors.initials: A
authors.lastname: Hua
authors.affiliation: First Affiliated Hospital, Guangxi University of Chinese Medicine, Nanning, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Dai
authors.affiliation: Department of Medicine, Rui Kang Hospital, Guangxi University of Chinese Medicine, Nanning, P.R. China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Lu
authors.affiliation: Department of Medicine, Rui Kang Hospital, Guangxi University of Chinese Medicine, Nanning, P.R. China.
authors.forename: Zhaolin
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Medicine, Rui Kang Hospital, Guangxi University of Chinese Medicine, Nanning, P.R. China.
authors.forename: Meilin
authors.initials: M
authors.lastname: Ye
authors.affiliation: National Cancer Institute, National Institutes of Health, Bethesda, MD, U.S.A.
authors.forename: Michael
authors.initials: M
authors.lastname: Weintraub
authors.affiliation: National Cancer Institute, National Institutes of Health, Bethesda, MD, U.S.A. liquenti@mail.nih.gov gxyelibin@sina.com.
authors.forename: Qingdi Quentin
authors.initials: QQ
authors.lastname: Li
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D002540:Cerebral Cortex / Q000098:blood supply* / Q000187:drug effects* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D007668:Kidney / Q000098:blood supply; D008099:Liver / Q000098:blood supply; D008297:Male; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D010477:Perfusion; D011817:Rabbits; D015427:Reperfusion Injury / Q000097:blood / Q000503:physiopathology* / Q000517:prevention & control*
chemical_list: D018696:Neuroprotective Agents
keywords: Cerebral dormancy; cerebral protective solution; rabbit animal model; waking up