title: P-Akt/miR‑200 signaling regulates epithelial-mesenchymal transition, migration and invasion in circulating gastric tumor cells.
abstract: Both circulating tumor cells (CTCs) and epithelial-mesenchymal transition (EMT) play an important role in invasion, migration and chemoresistant in tumor development. This study aimed to detect whether EMT occurred in human gastric CTCs and to explore the mechanism of EMT in human gastric CTCs. We analysed epithelial markers (pan-CK, E-cadherin), mesenchymal markers (N-cadherin, vimentin) EMT related miR‑200s, and Akt in gastric CTCs. The impact of miR‑200s on EMT, migration and invasion in CTCs was tested. We found that epithelial markers pan-CK, E-cadherin were decreased, and mesenchymal markers N-cadherin, vimentin were overexpressed in gastric CTCs. Expression of EMT related transcriptors, snail1, zeb1, twist1, were reversely correlated with miR‑200s, and were positively correlated with phospho-Akt. Upregulated of miR‑200s downregulated twist1 and zeb1 mRNA expression, and resulted in the supression of EMT, and impaired migration and invasion in gastric CTCs. Inhibition of p-Akt led to upregulation of miR‑200s. In conclusion, gastric CTCs exhibited remarkable EMT process, and p-Akt/miR‑200s signaling regulates EMT, migration and invasion in gastric CTCs.
authors.affiliation: Department of Medical Oncology, West China Hospital, Sichuan University, Chengdu 610041, P.R. China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Yuan
authors.affiliation: Laboratory of Signal Transduction and Molecular Target Therapy, West China Hospital, Sichuan University, Chengdu 610041, P.R. China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Xia
authors.affiliation: Laboratory of Signal Transduction and Molecular Target Therapy, West China Hospital, Sichuan University, Chengdu 610041, P.R. China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Laboratory of Signal Transduction and Molecular Target Therapy, West China Hospital, Sichuan University, Chengdu 610041, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Medical Oncology, West China Hospital, Sichuan University, Chengdu 610041, P.R. China.
authors.forename: Weibing
authors.initials: W
authors.lastname: Leng
authors.affiliation: Laboratory of Stem Cell Biology, West China Hospital, Sichuan University, Chengdu 610041, P.R. China.
authors.forename: Tie
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Medical Oncology, West China Hospital, Sichuan University, Chengdu 610041, P.R. China.
authors.forename: Qingjuan
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Laboratory of Signal Transduction and Molecular Target Therapy, West China Hospital, Sichuan University, Chengdu 610041, P.R. China.
authors.forename: Qiulin
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Laboratory of Stem Cell Biology, West China Hospital, Sichuan University, Chengdu 610041, P.R. China.
authors.forename: Xianming
authors.initials: X
authors.lastname: Mo
authors.affiliation: Department of Medical Oncology, West China Hospital, Sichuan University, Chengdu 610041, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Medical Oncology, West China Hospital, Sichuan University, Chengdu 610041, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Bi
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis; D009360:Neoplastic Cells, Circulating / Q000378:metabolism* / Q000473:pathology; D051058:Oncogene Protein v-akt / Q000096:biosynthesis* / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D009363:Neoplasm Proteins; D051058:Oncogene Protein v-akt