title: Characterization of zebrafish Pax1b and Pax9 in fin bud development.
abstract: Both Pax1 and Pax9 belong to the important paired box gene family (PAX), which mainly participates in animal development and sclerotome differentiation. To date, the precise molecular mechanism and related signaling pathway of Pax1 remain unclear. In our study, microinjection of morpholino- (MO-) modified antisense oligonucleotides against pax1b induced pectoral fin bud defects. Furthermore, we demonstrate that the phenotypes caused by the knockdown of Pax1b in zebrafish could not be phenocopied by pax9 MO and could not be rescued by either Pax1a or Pax9 overexpression. We further find that Pax1b affects the expression of col2a1, Uncx4.1, Noggin3, and aggrecan, confirming the role of Pax1b in chondrocyte differentiation and bone maturation. Moreover, we identify an interaction between PAX1 and FOXO1 and find that the interaction was enhanced under hypoxia stress. Together, this evidence for cell death caused by pax1b knockdown provides new insight into the role of the Pax protein family in cell fate determination and tissue specification.
authors.affiliation: Faculty of Basic Medical Sciences, Chongqing Medical University, Medical College Road 1, Chongqing 400016, China ; Emergency Department, The First Affiliated Hospital of Chongqing Medical University, Youyi Road 1, Chongqing 400042, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Chen
authors.affiliation: Faculty of Basic Medical Sciences, Chongqing Medical University, Medical College Road 1, Chongqing 400016, China.
authors.forename: Huizhe
authors.initials: H
authors.lastname: Huang
authors.affiliation: School of Life Sciences, Xiamen University, South Xiang'an Road, Xiamen 361102, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: Faculty of Basic Medical Sciences, Chongqing Medical University, Medical College Road 1, Chongqing 400016, China.
authors.forename: Fengjin
authors.initials: F
authors.lastname: Guo
authors.affiliation: Department of Nephrology, The First Affiliated Hospital of Chongqing Medical University, Youyi Road 1, Chongqing 400042, China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Du
authors.affiliation: Faculty of Basic Medical Sciences, Chongqing Medical University, Medical College Road 1, Chongqing 400016, China.
authors.forename: Linqiang
authors.initials: L
authors.lastname: Ma
authors.affiliation: Faculty of Basic Medical Sciences, Chongqing Medical University, Medical College Road 1, Chongqing 400016, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Faculty of Basic Medical Sciences, Chongqing Medical University, Medical College Road 1, Chongqing 400016, China.
authors.forename: Zhuma
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Faculty of Basic Medical Sciences, Chongqing Medical University, Medical College Road 1, Chongqing 400016, China ; Emergency Department, The First Affiliated Hospital of Chongqing Medical University, Youyi Road 1, Chongqing 400042, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Gui
authors.affiliation: Faculty of Basic Medical Sciences, Chongqing Medical University, Medical College Road 1, Chongqing 400016, China.
authors.forename: Taixian
authors.initials: T
authors.lastname: Yuan
authors.affiliation: School of Life Sciences, Xiamen University, South Xiang'an Road, Xiamen 361102, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Life Sciences, Xiamen University, South Xiang'an Road, Xiamen 361102, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Song
authors.affiliation: School of Life Sciences, Xiamen University, South Xiang'an Road, Xiamen 361102, China.
authors.forename: Yiquan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Nephrology, The First Affiliated Hospital of Chongqing Medical University, Youyi Road 1, Chongqing 400042, China.
authors.forename: Junling
authors.initials: J
authors.lastname: He
authors.affiliation: Faculty of Basic Medical Sciences, Chongqing Medical University, Medical College Road 1, Chongqing 400016, China ; Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Youyi Road 1, Chongqing 400042, China.
authors.forename: Han
authors.initials: H
authors.lastname: Lei
authors.affiliation: Faculty of Basic Medical Sciences, Chongqing Medical University, Medical College Road 1, Chongqing 400016, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Gao
mesh_terms: D058500:Animal Fins / Q000002:abnormalities / Q000196:embryology* / Q000378:metabolism*; D000818:Animals; D001846:Bone Development; D016923:Cell Death; D047108:Embryonic Development / Q000235:genetics; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D009024:Morphogenesis; D051764:PAX9 Transcription Factor / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D051761:Paired Box Transcription Factors / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D011485:Protein Binding; D013312:Stress, Physiological; D013623:Tail / Q000002:abnormalities; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; D051764:PAX9 Transcription Factor; D051761:Paired Box Transcription Factors; C000591521:Pax1b protein, zebrafish; D029961:Zebrafish Proteins; C576354:foxo1a protein, zebrafish; C497324:pax9 protein, zebrafish