title: Characterization of 10 microsatellite loci for Bathysa australis (Rubiaceae).
abstract: OBJECTIVE
  Bathysa australis is a common subcanopy tree from the Atlantic Forest that is pollinated by bees and wasps and produces autochoric seeds. This species exhibits great phenotypic plasticity along the elevational gradient of Serra do Mar in southeastern Brazil. We expect to assess the genetic diversity and gene flow between populations of this species along the elevational gradient. •
  
  
  RESULTS
  We developed a microsatellite-enriched genomic library for B. australis, and 10 microsatellite loci were successfully amplified, ranging from one to 13 alleles per locus. The observed and expected heterozygosities ranged from 0.333 to 0.900 (average: 0.629) and 0.564 to 0.900 (average: 0.742), respectively. •
  
  
  CONCLUSIONS
  These are the first microsatellite markers developed for the genus Bathysa and may be useful in other species of the Condamineeae tribe. These primers will be an important tool for studies of population ecology and conservation genetics.
authors.affiliation: Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, P.O. Box 6109, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Talita S
authors.initials: TS
authors.lastname: Reis
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, P.O. Box 6010, 13083-875 Campinas, São Paulo, Brazil ; Departamento de Fitopatologia, Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ)/Universidade de São Paulo, 12418-900 Piracicaba, São Paulo, Brazil.
authors.forename: Maísa
authors.initials: M
authors.lastname: Ciampi-Guillardi
authors.affiliation: Universidade Federal Rural do Semiárido, 59625-900 Mossoró, Rio Grande do Norte, Brazil.
authors.forename: Cristina
authors.initials: C
authors.lastname: Baldauf
authors.affiliation: Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, P.O. Box 6109, 13083-970 Campinas, São Paulo, Brazil ; Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, P.O. Box 6010, 13083-875 Campinas, São Paulo, Brazil.
authors.forename: Anete P
authors.initials: AP
authors.lastname: Souza
authors.affiliation: Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, P.O. Box 6109, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Flavio A M
authors.initials: FA
authors.lastname: Dos Santos
keywords: Atlantic Forest; Bathysa australis; conservation genetics; medicinal plant; polymorphism; population ecology