title: MiR-451 increases radiosensitivity of nasopharyngeal carcinoma cells by targeting ras-related protein 14 (RAB14).
abstract: Radioresistance severely impedes the treatment of nasopharyngeal carcinoma (NPC). Recent evidence has shown that the abnormal expression of microRNAs (miRNAs) contributes to radiosensitivity. The aim of this study, therefore, was to investigate whether expression of the miRNAs correlated with radiosensitivity in the context of NPC. Quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to quantify miR-451 expression in two representative NPC cell lines. The role of miR-451 in NPC radiosensitivity was analyzed using a colony formation assay and an immunofluorescence assay with overexpression of miR-451 in NPC cells. Luciferase reporter assays, RT-PCR, and Western blot were performed to confirm the target of miR-451. High levels of miR-451 expression enhanced radiosensitivity in NPC cells by inhibiting the repair of irradiation-induced double-strand breaks (DSBs) and increasing apoptosis. The results also demonstrated that miR-451 directly targeted ras-related protein 14 (RAB14). Downregulation of RAB14 partially replicated the miR-451-mediated DSBs induced by ionizing radiation (IR). MiR-451 could be a potential target for enhancing radiosensitivity of NPC cells by targeting RAB14.
authors.affiliation: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, No. 1838 Guangzhou Da Dao Bei, Guangzhou, Guangdong, 510515, People's Republic of China, crystal_sine@163.com.
authors.forename: Tian
authors.initials: T
authors.lastname: Zhang
authors.forename: Quanquan
authors.initials: Q
authors.lastname: Sun
authors.forename: Tongxin
authors.initials: T
authors.lastname: Liu
authors.forename: Jiarong
authors.initials: J
authors.lastname: Chen
authors.forename: Shasha
authors.initials: S
authors.lastname: Du
authors.forename: Chen
authors.initials: C
authors.lastname: Ren
authors.forename: Guixiang
authors.initials: G
authors.lastname: Liao
authors.forename: Yawei
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D002277:Carcinoma; D045744:Cell Line, Tumor; D004307:Dose-Response Relationship, Radiation; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics*; D034622:RNA Interference; D011836:Radiation Tolerance / Q000235:genetics*; D020691:rab GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; C531201:MIRN451 microRNA, human; D035683:MicroRNAs; C553411:Rab14 protein, human; D020691:rab GTP-Binding Proteins