title: Neuropeptide Y and leptin receptor expression in the hypothalamus of rats with chronic immobilization stress.
abstract: In this study, Sprague-Dawley rats were immobilized to a frame for 3 hours a day for 21 days to establish a model of chronic immobilization stress. The body weight and food intake of rats subjected to chronic immobilization stress were significantly decreased compared with the control group. Dual-labeling immunofluorescence revealed that the expression of leptin receptor and the co-localization coeffient in these leptic receptor neurons in the arcuate nucleus of the hypothalamus were both upregulated, while the number of neuropeptide Y neurons was decreased. Chronic immobilization stress induced high expression of leptin receptor in the arcuate nucleus and suppressed the synthesis and secretion of neuropeptide Y, thereby disrupting the pathways in the arcuate nucleus that regulate feeding behavior, resulting in diminished food intake and reduced body weight.
authors.affiliation: School of Pre-clinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China ; Traditional Chinese Medicine College of Hebei Medical University, Shijiazhang 050000, Hebei Province, China.
authors.forename: Shaoxian
authors.initials: S
authors.lastname: Wang
authors.affiliation: School of Pre-clinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Jiaxu
authors.initials: J
authors.lastname: Chen
authors.affiliation: Institute of Basic Theory in Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Guangxin
authors.initials: G
authors.lastname: Yue
authors.affiliation: School of Pre-clinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Bai
authors.affiliation: School of Pre-clinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Meijing
authors.initials: M
authors.lastname: Kou
authors.affiliation: School of Pre-clinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Zhongye
authors.initials: Z
authors.lastname: Jin
keywords: arcuate nucleus; body weight; chronic immobilization stress; double immunofluorescence; feeding center; grants-supported paper; hypothalamus; leptin receptor; neural regeneration; neuropeptide Y; neuroregeneration