title: Quantitative analysis of single-molecule superresolution images.
abstract: This review highlights the quantitative capabilities of single-molecule localization-based superresolution imaging methods. In addition to revealing fine structural details, the molecule coordinate lists generated by these methods provide the critical ability to quantify the number, clustering, and colocalization of molecules with 10-50 nm resolution. Here we describe typical workflows and precautions for quantitative analysis of single-molecule superresolution images. These guidelines include potential pitfalls and essential control experiments, allowing critical assessment and interpretation of superresolution images.
authors.affiliation: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Carla
authors.initials: C
authors.lastname: Coltharp
authors.affiliation: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Xinxing
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: xiao@jhmi.edu.
authors.forename: Jie
authors.initials: J
authors.lastname: Xiao
mesh_terms: D005456:Fluorescent Dyes; D008853:Microscopy / Q000379:methods* / Q000592:standards; D057054:Molecular Imaging / Q000379:methods* / Q000592:standards
chemical_list: D005456:Fluorescent Dyes