title: Overlapping between CYP3A4 and CYP3A7 expression in the fetal human liver during development.
abstract: OBJECTIVE
  The cytochrome P450 (CYP450) superfamily is implicated in important life processes, including metabolism of many molecules. CYP3A account for the largest portion of CYP450 proteins in human, including CYP3A4, CYP3A5 and CYP3A7. The purpose of this study was to investigate the immunohistochemical expression of CYP3A4 and CYP3A7 in human liver at different post-conceptional (PC) ages.
  
  
  METHODS
  Human liver samples from 30 fetuses and newborns were, clustered according with the PC age, routinely processed for immunohistochemical analysis of CYP3A4 and CYP3A7.
  
  
  RESULTS
  CYP3A4 was positive in all but two cases, CYP3A7 was positive in all but one case, which was negative also for CYP3A4.
  
  
  CONCLUSIONS
  Our data on immunohistochemical detection of CYP3A4 and CYP3A7 during development show that CYP3A4 expression is not restricted to the post-natal age, being the immunostaining for both CYP3A4 and CYP3A7 identical after 25 weeks of PC age, thus the relationship between these CYP450 isoforms should be considered much more complex than previous thought. A high interindividual variability was observed among subjects at all gestational age. The variable CYP3A expression suggests the existence of a marked interindividual variability in drug metabolism during the intrauterine life and in perinatal period.
authors.affiliation: a Department of Surgical Sciences, Divison of Pathology , University Hospital San Giovanni di Dio, University of Cagliari , Cagliari , Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Fanni
authors.affiliation: b Department of Pediatrics and Clinical Medicine , NICU, Puericulture Institute and Neonatal Section, University of Cagliari , Cagliari , Italy.
authors.forename: Vassillios
authors.initials: V
authors.lastname: Fanos
authors.affiliation: a Department of Surgical Sciences, Divison of Pathology , University Hospital San Giovanni di Dio, University of Cagliari , Cagliari , Italy.
authors.forename: Rossano
authors.initials: R
authors.lastname: Ambu
authors.affiliation: a Department of Surgical Sciences, Divison of Pathology , University Hospital San Giovanni di Dio, University of Cagliari , Cagliari , Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Lai
authors.affiliation: a Department of Surgical Sciences, Divison of Pathology , University Hospital San Giovanni di Dio, University of Cagliari , Cagliari , Italy.
authors.forename: Clara
authors.initials: C
authors.lastname: Gerosa
authors.affiliation: a Department of Surgical Sciences, Divison of Pathology , University Hospital San Giovanni di Dio, University of Cagliari , Cagliari , Italy.
authors.forename: Pietro
authors.initials: P
authors.lastname: Pampaloni
authors.affiliation: c Pathology Department , ZOL (Ziekenhuis Oost-Limburg) , Genk , Belgium , and.
authors.forename: Peter
authors.initials: P
authors.lastname: Van Eyken
authors.affiliation: a Department of Surgical Sciences, Divison of Pathology , University Hospital San Giovanni di Dio, University of Cagliari , Cagliari , Italy.
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Senes
authors.affiliation: d Faculty of Medicine , Institute of Biochemistry and Clinical Biochemistry, Catholic University , Rome , Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Castagnola
authors.affiliation: a Department of Surgical Sciences, Divison of Pathology , University Hospital San Giovanni di Dio, University of Cagliari , Cagliari , Italy.
authors.forename: Gavino
authors.initials: G
authors.lastname: Faa
keywords: CYP3A4; CYP3A7; cytochrome P450; fetus; immunohistochemistry; liver; newborns