title: Functional characterization of chemosensory proteins in the scarab beetle, Holotrichia oblita Faldermann (Coleoptera: Scarabaeida).
abstract: Chemosensory proteins (CSPs) play important roles in chemical communication by insects, as they recognize and transport environmental chemical signals to receptors within sensilla. In this study, we identified HoblCSP1 and HoblCSP2 from a cDNA library of Holotrichia oblita antennae, successfully expressed them in E. coli and purified them by Ni ion affinity chromatography. We then measured the ligand-binding specificities of HoblCSP1 and HoblCSP2 to 50 selected ligands in a competitive binding assay. These results demonstrated that HoblCSP1 and HoblCSP2 have similar ligand-binding spectra. Both proteins displayed the highest affinity for β-ionone, α-ionone and cinnamaldehyde, indicating that they prefer binding to odorants other than sex pheromones. Additionally, immuno-localization revealed that HoblCSP1 is highly concentrated in sensilla basiconica, while HoblCSP2 is specifically localized to sensilla placodea. In conclusion, HoblCSP1 and HoblCSP2 are responsible for binding to general odorants with slightly different specificities due to their different in vivo environments.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China; College of Plant Science, Jilin University, Changchun, Jilin Province, P.R. China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Sun
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China; College of Plant Science, Jilin University, Changchun, Jilin Province, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Guan
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China; College of Plant Science, Jilin University, Changchun, Jilin Province, P.R. China.
authors.forename: Honglin
authors.initials: H
authors.lastname: Feng
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Yin
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China.
authors.forename: Yazhong
authors.initials: Y
authors.lastname: Cao
authors.affiliation: College of Plant Science, Jilin University, Changchun, Jilin Province, P.R. China.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Xi
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China.
authors.forename: Kebin
authors.initials: K
authors.lastname: Li
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism* / Q000648:ultrastructure; D001667:Binding, Competitive; D001517:Coleoptera / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D008297:Male; D008969:Molecular Sequence Data; D009475:Neurons, Afferent / Q000166:cytology / Q000378:metabolism; D045792:Norisoprenoids / Q000737:chemistry / Q000378:metabolism; D009812:Odorants / Q000032:analysis; D010802:Phylogeny; D011485:Protein Binding; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D058618:Sensilla / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D019476:Insect Proteins; D008024:Ligands; D045792:Norisoprenoids; D018035:Receptors, Odorant; D011994:Recombinant Proteins; D000171:Acrolein; C008157:beta-ionone; C011879:alpha-ionone; C012843:cinnamaldehyde