title: Genistein inhibits the replication of avian leucosis virus subgroup J in DF-1 cells.
abstract: To investigate the antiviral effects of genistein on the replication of avian leukosis virus subgroup J (ALV-J) in DF-1 cells, the cells were treated with genistein at different time points and the antiviral effects were examined by using a variety of assays. We determined that genistein strongly inhibited viral gene expression and decreased the viral protein level in the cell supernatant and the cytoplasm without alerting virus receptor expression and viral attachment. We also observed that genistein was not found to interfere with virus entry, but significantly inhibited both viral gene transcriptions at 24h post infection and virus release, which indicate that genistein exerts its inhibitory effects on the late phase of ALV-J replicative cycle. These results demonstrate that genistein effectively block ALV-J replication by inhibiting virus transcription and release in DF-1 cells, which may be useful for therapeutic drug design.
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou 225009, China; Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. Electronic address: qiankun@yzu.edu.cn.
authors.forename: Kun
authors.initials: K
authors.lastname: Qian
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou 225009, China. Electronic address: 1037034639@qq.com.
authors.forename: Ai-jun
authors.initials: AJ
authors.lastname: Gao
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou 225009, China. Electronic address: 920557300@qq.com.
authors.forename: Ming-yue
authors.initials: MY
authors.lastname: Zhu
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou 225009, China; Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. Electronic address: 987137092@qq.com.
authors.forename: Hong-xia
authors.initials: HX
authors.lastname: Shao
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou 225009, China; Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. Electronic address: 415075168@qq.com.
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Jin
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou 225009, China; Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. Electronic address: 2577853657@qq.com.
authors.forename: Jian-qiang
authors.initials: JQ
authors.lastname: Ye
authors.affiliation: Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou 225009, China; Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. Electronic address: aijian@yzu.edu.cn.
authors.forename: Ai-jian
authors.initials: AJ
authors.lastname: Qin
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001354:Avian Leukosis Virus / Q000187:drug effects* / Q000502:physiology*; D002460:Cell Line; D002645:Chickens; D019833:Genistein / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D014158:Transcription, Genetic / Q000187:drug effects; D057074:Virus Release / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D019833:Genistein
keywords: Avian leucosis virus subgroup J; DF-1 cell; Genistein; Inhibition; Virus replication