title: Novel microsatellite loci for Sebaea aurea (Gentianaceae) and cross-amplification in related species.
abstract: OBJECTIVE
  Microsatellite loci were developed in Sebaea aurea (Gentianaceae) to investigate the functional role of diplostigmaty (i.e., the presence of additional stigmas along the style). •
  
  
  RESULTS
  One hundred seventy-four and 180 microsatellite loci were isolated through 454 shotgun sequencing of genomic and microsatellite-enriched DNA libraries, respectively. Sixteen polymorphic microsatellite loci were characterized, and 12 of them were selected to genotype individuals from two populations. Microsatellite amplification was conducted in two multiplex groups, each containing six microsatellite loci. Cross-species amplification was tested in seven other species of Sebaea. The 12 novel microsatellite loci amplified only in the two most closely related species to S. aurea (i.e., S. ambigua and S. minutiflora) and were also polymorphic in these two species. •
  
  
  CONCLUSIONS
  These results demonstrate the usefulness of this set of newly developed microsatellite loci to investigate the mating system and population genetic structure in S. aurea and related species.
authors.affiliation: Institute of Biology, University of Neuchâtel, Rue Emile-Argand 11, 2000 Neuchâtel, Switzerland ; Department of Ecology and Evolution, University of Lausanne, 1015 Lausanne, Switzerland.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Kissling
authors.affiliation: Institute of Biology, University of Neuchâtel, Rue Emile-Argand 11, 2000 Neuchâtel, Switzerland.
authors.forename: Olivier
authors.initials: O
authors.lastname: Bachmann
authors.affiliation: Ecogenics GmbH, Grabenstrasse 11a, 8952 Zürich-Schlieren, Switzerland.
authors.forename: Marco R
authors.initials: MR
authors.lastname: Thali
authors.affiliation: Centro de Investigaciones sobre Desertificación (CIDE-CSIC-UV-GV), Carretera de Moncada-Náquera, Km 4.5, E-46113 Moncada, Valencia, Spain.
authors.forename: José Gabriel
authors.initials: JG
authors.lastname: Segarra-Moragues
keywords: Gentianaceae; Sebaea aurea; diplostigmaty; microsatellites; multiplex