title: Development and characterization of 11 polymorphic microsatellite markers in Tapiscia sinensis (Staphyleaceae).
abstract: OBJECTIVE
  We developed and characterized five polymorphic microsatellite loci from an AC-enriched genomic library of a rare tree, Tapiscia sinensis, and six compound microsatellite primers using a dual-suppression-PCR-based approach, to investigate geographical genetic structure. •
  
  
  RESULTS
  The polymorphism of these loci was assessed in 102 samples collected from three populations. The number of alleles per locus ranged from one to nine. Expected heterozygosity per locus ranged from 0.000 to 0.709 and observed heterozygosity ranged from 0.000 to 0.750. Five loci deviated from Hardy-Weinberg equilibrium after Bonferroni correction. •
  
  
  CONCLUSIONS
  This set of microsatellites may be useful for investigating broad-scale genetic variation in the extant populations of the endangered tree species T. sinensis.
authors.affiliation: College of Horticulture, Shanxi Agriculture University, Taigu 030801, Shanxi, People's Republic of China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Zhang
authors.affiliation: College of Horticulture, Shanxi Agriculture University, Taigu 030801, Shanxi, People's Republic of China.
authors.forename: Zhiguang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Life Science, Shanxi Agriculture University, Taigu 030801, Shanxi, People's Republic of China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, Hubei, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Tian
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, Hubei, People's Republic of China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Yao
authors.affiliation: College of Life Sciences, Jiangxi Normal University, Nanchang 330022, Jiangxi, People's Republic of China.
authors.forename: Jinju
authors.initials: J
authors.lastname: Zhang
keywords: Staphyleaceae; Tapiscia sinensis; conservation genetics; microsatellite