title: Preparation and characterization of antiadhesion barrier film from hyaluronic acid-grafted electrospun poly(caprolactone) nanofibrous membranes for prevention of flexor tendon postoperative peritendinous adhesion.
abstract: Peritendinous adhesion is one of the common complications encountered after tendon injury and subsequent surgery, and it can be minimized by introducing a physical barrier between the injured site and the surrounding tissue. An electrospun hyaluronic acid-grafted poly(caprolactone) (PCL-g-HA) nanofibrous membrane (NFM) is proposed as an alternative to current antiadhesion barrier films. HA is covalently grafted to surface-aminolyzed PCL nanofibers, using carbodiimide as the coupling agent. Pristine PCL and PCL-g-HA NFMs were characterized by scanning electron microscopy, thermogravimetric analysis, X-ray photoelectron spectroscopy, Fourier-transform infrared spectroscopy, and mechanical testing. In vitro cell culture with fibroblasts showed that PCL-g-HA NFMs reduced cellular adhesion on the membrane surface while maintaining cell proliferation. Animal experiments using a rabbit flexor digitorum profundus tendon model confirmed the efficacy of PCL-g-HA in reducing peritendinous adhesion, based on gross observation, histology, joint flexion-angle measurements, gliding tests, and biomechanical evaluation.
authors.affiliation: Department of Chemical and Materials Engineering, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taiwan, Republic of China.
authors.forename: Shih-Hsien
authors.initials: SH
authors.lastname: Chen
authors.affiliation: Department of Chemical and Materials Engineering, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taiwan, Republic of China ; Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taiwan, Republic of China.
authors.forename: Chih-Hao
authors.initials: CH
authors.lastname: Chen
authors.affiliation: Department of Chemical and Materials Engineering, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taiwan, Republic of China.
authors.forename: K T
authors.initials: KT
authors.lastname: Shalumon
authors.affiliation: Department of Chemical and Materials Engineering, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taiwan, Republic of China ; Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan, Republic of China.
authors.forename: Jyh-Ping
authors.initials: JP
authors.lastname: Chen
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D008567:Membranes, Artificial; D057139:Nanofibers / Q000737:chemistry* / Q000627:therapeutic use; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D011817:Rabbits; D013710:Tendons / Q000187:drug effects / Q000601:surgery; D000267:Tissue Adhesions / Q000188:drug therapy / Q000517:prevention & control*
chemical_list: D008567:Membranes, Artificial; D011091:Polyesters; C118198:Seprafilm; C016240:polycaprolactone; D006820:Hyaluronic Acid
keywords: antiadhesion; barrier film; hyaluronic acid; nanofibrous membranes; peritendinous adhesion; polycaprolactone; surface grafting