title: Morphologic and proliferative characteristics of goat type A spermatogonia in the presence of different sets of growth factors.
abstract: OBJECTIVE
  The present study by using different growth factors was aimed to develop the best practical culture condition for purification of goat undifferentiated SSCs and their colonization under in vitro and in vivo conditions.
  
  
  METHODS
  The enzymatically isolated SSCs obtained from one month old goat testes were cultured in DMEM plus FCS supplemented with different sets of growth factors (GDNF, LIF, bFGF, and EGF) for 2 weeks. At the end of each week, the morphological characteristics of cells and colonies alongside with purification rate of undifferentiated type A spermatogonia were evaluated by immunocytochemical staining and flow cytometry.
  
  
  RESULTS
  The number and size of colonies in treatment groups were significantly (P < 0.01) higher than corresponding values in control group. In immunocytochemical evaluation, the proportion of KIT and PGP9.5 positive cells were significantly (P < 0.001) higher in control and treatment groups, respectively.
  
  
  CONCLUSIONS
  The culture medium comprising all four growth factors, especially the one supplemented with the higher concentration of GDNF, was superior to the other groups with respect to the population of undifferentiated type A spermatogonia and its propagation in culture system. Additionally, goat SSCs could colonize within the mouse testis following xenotransplantation.
authors.affiliation: Reproductive Biotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran.
authors.forename: Mohammad Sadra
authors.initials: MS
authors.lastname: Shirazi
authors.forename: Banafsheh
authors.initials: B
authors.lastname: Heidari
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Shirazi
authors.forename: Amir Hassan
authors.initials: AH
authors.lastname: Zarnani
authors.forename: Mahmood
authors.initials: M
authors.lastname: Jeddi-Tehrani
authors.forename: Maryam
authors.initials: M
authors.lastname: Rahmati-Ahmadabadi
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Naderi
authors.forename: Bahareh
authors.initials: B
authors.lastname: Behzadi
authors.forename: Moretza
authors.initials: M
authors.lastname: Farab
authors.forename: Ali
authors.initials: A
authors.lastname: Sarvari
authors.forename: Sara
authors.initials: S
authors.lastname: Borjian-Boroujeni
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Akhondi
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000662:veterinary; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003470:Culture Media; D006041:Goats; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D008297:Male; D051379:Mice; D013093:Spermatogonia / Q000166:cytology* / Q000378:metabolism; D013737:Testis / Q000166:cytology; D014183:Transplantation, Heterologous / Q000662:veterinary
chemical_list: D003470:Culture Media; D036341:Intercellular Signaling Peptides and Proteins