title: Antibacterial synergy of glycerol monolaurate and aminoglycosides in Staphylococcus aureus biofilms.
abstract: Glycerol monolaurate (GML) is a natural surfactant with antimicrobial properties. At ∼0.3 mM, both GML and its component lauric acid were bactericidal for antibiotic-resistant Staphylococcus aureus biofilms. With the use of MICs of antibiotics obtained from planktonic cells, GML and lauric acid acted synergistically with gentamicin and streptomycin, but not ampicillin or vancomycin, to eliminate detectable viable biofilm bacteria. Images of GML-treated biofilms suggested that GML may facilitate antibiotic interaction with matrix-embedded bacteria.
authors.affiliation: Department of Surgery, University of Minnesota, Minneapolis, Minnesota, USA Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota, USA.
authors.forename: Donavon J
authors.initials: DJ
authors.lastname: Hess
authors.affiliation: Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, USA.
authors.forename: Michelle J
authors.initials: MJ
authors.lastname: Henry-Stanley
authors.affiliation: Department of Surgery, University of Minnesota, Minneapolis, Minnesota, USA Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, USA wells002@umn.edu.
authors.forename: Carol L
authors.identifier: http://orcid.org/0000-0003-4842-2678
authors.initials: CL
authors.lastname: Wells
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology*; D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D005839:Gentamicins / Q000494:pharmacology; D007848:Laurates / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D050178:Monoglycerides / Q000494:pharmacology*; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects*; D013307:Streptomycin / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D005839:Gentamicins; D007848:Laurates; D050178:Monoglycerides; D013501:Surface-Active Agents; C020777:monolaurin; D014640:Vancomycin; D000667:Ampicillin; D013307:Streptomycin