title: Comparative metabolomic-based metabolic mechanism hypothesis for microbial mixed cultures utilizing cane molasses wastewater for higher 2-phenylethanol production.
abstract: The mixed microbes coculture method in cane molasses wastewater (CMW) was adopted to produce 2-phenylethanol (2-PE). Comparative metabolomics combined with multivariate statistical analysis was performed to profile the differences of overall intracellular metabolites concentration for the mixed microbes cocultured under two different fermentation conditions with low and high 2-PE production. In total 102 intracellular metabolites were identified, and 17 of them involved in six pathways were responsible for 2-PE biosynthesis. After further analysis of metabolites and verification by feeding experiment, an overall metabolic mechanism hypothesis for the microbial mixed cultures (MMC) utilizing CMW for higher 2-PE production was presented. The results demonstrated that the branches of intracellular pyruvate metabolic flux, as well as the flux of phenylalanine, tyrosine, tryptophan, glutamate, proline, leucine, threonine, and oleic acid, were closely related to 2-PE production and cell growth, which provided theoretical guidance for domestication and selection of species as well as medium optimization for MMC metabolizing CMW to enhance 2-PE yield.
authors.affiliation: Key Laboratory of Systems Bioengineering (Ministry of Education) and SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, People's Republic of China.
authors.forename: Xinrong
authors.initials: X
authors.lastname: Pan
authors.forename: Haishan
authors.initials: H
authors.lastname: Qi
authors.forename: Li
authors.initials: L
authors.lastname: Mu
authors.forename: Jianping
authors.initials: J
authors.lastname: Wen
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Jia
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology; D002952:Citric Acid Cycle; D005227:Fatty Acids / Q000378:metabolism; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D008965:Molasses; D010427:Pentose Phosphate Pathway; D010626:Phenylethyl Alcohol / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism; D012765:Shikimic Acid / Q000378:metabolism; D062065:Wastewater
chemical_list: D000596:Amino Acids; D005227:Fatty Acids; D010626:Phenylethyl Alcohol; D019289:Pyruvic Acid; D012765:Shikimic Acid; D062065:Wastewater
keywords: 2-phenyethanol; cane molasses wastewater; comparative metabolomics; metabolic mechanism; mixed microbes