title: Nogo-B receptor expression correlates negatively with malignancy grade and ki-67 antigen expression in invasive ductal breast carcinoma.
abstract: BACKGROUND
  Nogo-B receptor (NgBR) has been shown to be involved in endothelial cell chemotaxis and morphogenesis. However, few studies analyzing its expression in cancer cells have been performed.
  
  
  METHODS
  We examined NgBR expression in 233 patients with invasive ductal breast carcinoma (IDC) and corresponding non-malignant breast tissues (NMBT) on mRNA (real-time polymerase chain reaction) and protein levels (immunohistochemistry; IHC and western-blot analysis). NgBR expression was found also analyzed in breast cancer cell lines of varying invasiveness.
  
  
  RESULTS
  NgBR expression was increased in IDC compared to NMBT on the mRNA (p=0.0007) and protein level (p=0.018). NgBR expression decreased significantly with IDC malignancy grade and correlated negatively with the Ki-67 antigen expression (r=-0.18; p=0.0005). High NgBR mRNA expression was associated with estrogen receptor negativity (p=0.0023) and the triple-negative phenotype of the tumors (p=0.0129).
  
  
  CONCLUSIONS
  NgBR may be involved in IDC development, however, its role in its progression requires further research.
authors.affiliation: Department of Histology and Embryology, Medical University, Wroclaw, Poland.
authors.forename: Bartosz
authors.initials: B
authors.lastname: Pula
authors.affiliation: Department of Histology and Embryology, Medical University, Wroclaw, Poland.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Olbromski
authors.affiliation: Department of Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Environmental and Life Sciences, Wroclaw, Poland Laboratory of Glycobiology and Cell Interactions, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Owczarek
authors.affiliation: Department of Tumour Pathology, Centre of Oncology, Maria Sklodowska-Curie Memorial Institute, Krakow, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Ambicka
authors.affiliation: Research and Development Center, Regional Specialist Hospital, Wroclaw, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Witkiewicz
authors.affiliation: Department of Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Environmental and Life Sciences, Wroclaw, Poland Laboratory of Glycobiology and Cell Interactions, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Ugorski
authors.affiliation: Department of Tumour Pathology, Centre of Oncology, Maria Sklodowska-Curie Memorial Institute, Krakow, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Rys
authors.affiliation: Department of Histology and Embryology, Medical University, Wroclaw, Poland Department of Histology and Embryology, Medical University, Poznan, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Zabel
authors.affiliation: Department of Histology and Embryology, Medical University, Wroclaw, Poland Department of Physiotherapy, University School of Physical Education, Wroclaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Dziegiel
authors.affiliation: Department of Histology and Embryology, Medical University, Wroclaw, Poland marzenna.podhorska-okolow@umed.wroc.pl.
authors.forename: Marzena
authors.initials: M
authors.lastname: Podhorska-Okolow
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000401:mortality / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000188:drug therapy / Q000378:metabolism* / Q000401:mortality / Q000473:pathology*; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D005500:Follow-Up Studies; D015870:Gene Expression; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism*; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D011379:Prognosis; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*
chemical_list: D019394:Ki-67 Antigen; C512614:NUS1 protein, human; D011956:Receptors, Cell Surface
keywords: Breast cancer; Ki-67 antigen; NgBR; Nogo-B receptor; immunohistochemistry