title: Activin A prevents neuron-like PC12 cell apoptosis after oxygen-glucose deprivation.
abstract: In this study, PC12 cells were induced to differentiate into neuron-like cells using nerve growth factor, and were subjected to oxygen-glucose deprivation. Cells were treated with 0, 10, 20, 30, 50, 100 ng/mL exogenous Activin A. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide assay and Hoechst 33324 staining showed that the survival percentage of PC12 cells significantly decreased and the rate of apoptosis significantly increased after oxygen-glucose deprivation. Exogenous Activin A significantly increased the survival percentage of PC12 cells in a dose-dependent manner. Reverse transcription-PCR results revealed a significant increase in Activin receptor IIA, Smad3 and Smad4 mRNA levels, which are key sites in the Activin A/Smads signaling pathway, in neuron-like cells subjected to oxygen-glucose deprivation, while mRNA expression of the apoptosis-regulation gene caspase-3 decreased. Our experimental findings indicate that exogenous Activin A plays an anti-apoptotic role and protects neurons by means of activating the Activin A/Smads signaling pathway.
authors.affiliation: Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin Province, China ; Department of Neurology, Changchun Central Hospital, Changchun 130051, Jilin Province, China.
authors.forename: Guihua
authors.initials: G
authors.lastname: Xu
authors.affiliation: Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors.forename: Jinting
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors.forename: Chunli
authors.initials: C
authors.lastname: Mei
authors.affiliation: Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors.forename: Jiaoqi
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors.forename: Zhongshu
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors.forename: Han
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Mang
authors.affiliation: Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin Province, China.
authors.forename: Zhongxin
authors.initials: Z
authors.lastname: Xu
keywords: Activin A; Activin A/Smads signaling pathway; apoptosis; biological factor; brain injury; caspase-3; grants-supported paper; neural regeneration; neuroregeneration; oxygen-glucose deprivation