title: Effect of ranolazine on cardiac microcirculation in normal and diabetic rats.
abstract: Ranolazine is a new antianginal drug that is postulated to act through blocking of cardiac late sodium channels and improvement in cardiac blood flow. The present study has been carried out to investigate its possible effect on microcirculation of normal and diabetic rats’ cardiac muscle.
  
  
  METHODS
  Normal and diabetic rats were anesthetized.The chest was opened and a surface laser Doppler flowmeter probe was sutured on the left ventricle next to the left anterior descending branch of the coronary artery. After 30 minutes of animal adaptation, baseline flow was recorded.Thereafter, ranolazine was injected intravenously (10 mg/kg) through the jugular vein and the changes in the cardiac blood flow were measured every 5 minutes for 30 minutes. Systolic blood pressure and heart rate were also recorded.
  
  
  RESULTS
  Although, injection of ranolazine resulted in some reduction of cardiac blood flow, no significant changes were observed in normal and diabetic animals. Ranolazine had no significant effect on systolic blood pressure of either groups studied. Nevertheless, it caused a mild but statistically significant increase in the heart rate of diabetic and normal rats.
  
  
  CONCLUSIONS
  In conclusion, in normal and diabetic rats, no evidence of improvement in intramyocardial blood flow by ranolazine was obtained.
authors.affiliation: Shiraz University of Medical Sciences Department of Pharmacology PO Box 71345-1649 Shiraz Iran.
authors.forename: H
authors.initials: H
authors.lastname: Khazraei
authors.affiliation: Shiraz University of Medical Sciences Department of Surgery, Division of Cardiovascular Surgery Shiraz Iran.
authors.forename: M
authors.initials: M
authors.lastname: Shafa
authors.affiliation: Shiraz University of Medical Sciences Department of Pharmacology PO Box 71345-1649 Shiraz Iran Shiraz University of Medical Sciences Medicinal and Natural Products Chemistry Research Center Shiraz Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Mirkhani
mesh_terms: D000083:Acetanilides / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D001783:Blood Flow Velocity; D001794:Blood Pressure / Q000187:drug effects; D002317:Cardiovascular Agents / Q000008:administration & dosage / Q000494:pharmacology*; D003326:Coronary Circulation / Q000187:drug effects*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D006339:Heart Rate / Q000187:drug effects; D007275:Injections, Intravenous; D017078:Laser-Doppler Flowmetry; D008297:Male; D008833:Microcirculation / Q000187:drug effects*; D010879:Piperazines / Q000008:administration & dosage / Q000494:pharmacology*; D000069458:Ranolazine; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D000083:Acetanilides; D002317:Cardiovascular Agents; D010879:Piperazines; D000069458:Ranolazine
keywords: cardiac microcirculation; diabetes; laser Doppler flowmetry; ranolazine; rat