title: Hypoxia regulates reactive oxygen species levels in SHG-44 glioma cells.
abstract: In the present study, cultured human SHG-44 glioma cells were subjected to a hypoxic environment simulated using the CoCl2 method. Flow cytometry showed increased reactive oxygen species production in these cells. Real-time reverse transcription-PCR showed significantly increased hypoxia-inducible factor-1α mRNA expression in cells exposed to the hypoxic condition. The antioxidant N-acetylcysteine significantly inhibited reactive oxygen species production and reduced hypoxia-inducible factor-1α mRNA expression in normoxic and hypoxic groups, especially in the latter group. These findings indicate that hypoxia induces reactive oxygen species production and hypoxia-inducible factor-1α mRNA expression in human SHG-44 glioma cells, and that the antioxidant N-acetylcysteine can inhibit these changes.
authors.affiliation: First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710000, Shaanxi Province, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Xianyang Central Hospital, Xianyang 712000, Shaanxi Province, China.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Xie
authors.affiliation: First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710000, Shaanxi Province, China.
authors.forename: Gaofeng
authors.initials: G
authors.lastname: Xu
authors.affiliation: Xianyang Central Hospital, Xianyang 712000, Shaanxi Province, China.
authors.forename: Yongyong
authors.initials: Y
authors.lastname: Su
authors.affiliation: Xianyang Central Hospital, Xianyang 712000, Shaanxi Province, China.
authors.forename: Jinzheng
authors.initials: J
authors.lastname: Li
authors.affiliation: Xi'an Central Hospital, Xi'an 710000, Shaanxi Province, China.
authors.forename: Mang
authors.initials: M
authors.lastname: Zhu
authors.affiliation: First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710000, Shaanxi Province, China.
authors.forename: Maode
authors.initials: M
authors.lastname: Wang
keywords: N-acetylcysteine; antioxidant; basic research; central nervous system; glioma; hypoxia; hypoxia-inducible factor-1α; neural regeneration; neuroregeneraion; photographs-containing paper; reactive oxygen species