title: Microsatellite loci for an old rare species, Pseudotaxus chienii, and transferability in Taxus wallichiana var. mairei (Taxaceae).
abstract: OBJECTIVE
  Microsatellite loci were developed for Pseudotaxus chienii, an old rare species endemic to China, and which provided a useful tool for investigating the patterns of population genetic structure, phylogeography, evolutionary history, and adaptive potential. Transferability was assayed in the related species, Taxus wallichiana var. mairei. •
  
  
  RESULTS
  A total of 15 microsatellite loci were targeted in P. chienii using the Fast Isolation by AFLP of Sequences COntaining Repeats (FIASCO) protocol. Polymorphism was evaluated in five populations of P. chienii and five populations of T. wallichiana var. mairei. Of these loci, 13 were polymorphic in P. chienii, whereas 15 were polymorphic in T. wallichiana var. mairei. •
  
  
  CONCLUSIONS
  The 15 microsatellite loci developed lay a solid foundation for further studies on population genetic variability and investigations of local adaptation. Additionally, cross-species amplification in T. wallichiana var. mairei showed that these loci may also have potential utility in other genera of Taxaceae.
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China ; Shenzhen R&D Center of State Key Laboratory of Biocontrol, Sun Yat-sen University, Shenzhen, People's Republic of China ; Institute for Technology Research and Innovation of Sun Yat-sen University, Zhuhai, People's Republic of China.
authors.forename: Ying-Juan
authors.initials: YJ
authors.lastname: Su
authors.affiliation: CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
keywords: Pseudotaxus chienii; Taxus wallichiana var. mairei; genetic diversity; microsatellites; transferability