title: Love wave immunosensor for the detection of carbaryl pesticide.
abstract: A Love Wave (LW) immunosensor was developed for the detection of carbaryl pesticide. The experimental setup consisted on: a compact electronic characterization circuit based on phase and amplitude detection at constant frequency; an automated flow injection system; a thermal control unit; a custom-made flow-through cell; and Quartz /SiO2 LW sensors with a 40 μm wavelength and 120 MHz center frequency. The carbaryl detection was based on a competitive immunoassay format using LIB-CNH45 monoclonal antibody (MAb). Bovine Serum Albumin-CNH (BSA-CNH) carbaryl hapten-conjugate was covalently immobilized, via mercaptohexadecanoic acid self-assembled monolayer (SAM), onto the gold sensing area of the LW sensors. This immobilization allowed the reusability of the sensor for at least 70 assays without significant signal losses. The LW immunosensor showed a limit of detection (LOD) of 0.09 μg/L, a sensitivity of 0.31 μg/L and a linear working range of 0.14-1.63 μg/L. In comparison to other carbaryl immunosensors, the LW immunosensor achieved a high sensitivity and a low LOD. These features turn the LW immunosensor into a promising tool for applications that demand a high resolution, such as for the detection of pesticides in drinking water at European regulatory levels.
authors.affiliation: Grupo de Fenómenos Ondulatorios (GFO), Universitat Politècnica de València, 46022 Valencia, Spain. laurent.francis@uclouvain.be.
authors.forename: María-Isabel
authors.initials: MI
authors.lastname: Rocha-Gaso
authors.affiliation: Grupo de Fenómenos Ondulatorios (GFO), Universitat Politècnica de València, 46022 Valencia, Spain. jogarnar@upvnet.upv.es.
authors.forename: José-Vicente
authors.initials: JV
authors.lastname: García
authors.affiliation: Grupo de Fenómenos Ondulatorios (GFO), Universitat Politècnica de València, 46022 Valencia, Spain. pablo@awsensors.com.
authors.forename: Pablo
authors.initials: P
authors.lastname: García
authors.affiliation: Instituto Interuniversitario de Investigación en Bioingeniería y Tecnología Orientada al Ser Humano (I3BH), Universitat Politècnica de València, 46022 Valencia, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: March-Iborra
authors.affiliation: Grupo de Fenómenos Ondulatorios (GFO), Universitat Politècnica de València, 46022 Valencia, Spain. yojiji@eln.upv.es.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Jiménez
authors.affiliation: Sensors, Microsystems and Actuators Laboratory of Louvain (SMALL), ICTEAM Institute, Université Catholique de Louvain, 1348 Louvain-la-Neuve, Belgium. laurent.francis@uclouvain.be.
authors.forename: Laurent-Alain
authors.initials: LA
authors.lastname: Francis
authors.affiliation: Instituto Interuniversitario de Investigación en Bioingeniería y Tecnología Orientada al Ser Humano (I3BH), Universitat Politècnica de València, 46022 Valencia, Spain. amontoya@ginmuno.i3bh.es.
authors.forename: Angel
authors.initials: A
authors.lastname: Montoya
authors.affiliation: Grupo de Fenómenos Ondulatorios (GFO), Universitat Politècnica de València, 46022 Valencia, Spain. aarnau@eln.upv.es.
authors.forename: Antonio
authors.initials: A
authors.lastname: Arnau
mesh_terms: D000162:Acoustics / Q000295:instrumentation*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D012721:Carbaryl / Q000032:analysis* / Q000276:immunology; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D010575:Pesticides / Q000032:analysis* / Q000276:immunology; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D010575:Pesticides; D014874:Water Pollutants, Chemical; D012721:Carbaryl