title: Effects of hypoxic exercise training on microRNA expression and lipid metabolism in obese rat livers.
abstract: To investigate the effects of hypoxic exercise training on microRNA (miRNA) expression and the role of miRNA expression in regulating lipid metabolism, 20 dietary-induced obese SD rats were divided into a normoxic sedentary group (N, n=10) and a hypoxic exercise training group (H, n=10). After four weeks, measurements were taken of body weight, body length, fat mass, serum lipid concentration, miRNAs differentially expressed in rat liver, and gene and protein expression levels of peroxisome proliferator activated receptor α (PPARα), fatty acid synthetase (FAS), and carnitine palmitoyl transferase 1A (CPT1A) in rat liver. Body weight, Lee's index, fat mass, fat/weight ratio, and serum levels of total cholesterol (TC) and high density lipoprotein cholesterol (HDL-C) were all significantly lower in the H group than in the N group (P<0.01). Six miRNAs expressed significantly differently in the liver (P<0.05). Specifically, expression levels of miR-378b were significantly lower in the H group than in the N group (P<0.05). Compared with the normoxic sedentary group, hypoxic exercise training resulted in a lower ratio of FAS mRNA to CPT1A mRNA (P<0.05), as well as lower CPT1A protein levels (P<0.01), while a higher ratio of FAS to CPT1A protein levels (P<0.01) was observed. In conclusion, hypoxic training may elevate the resistance of high fat diet induced obesity in rats by reducing the expression of miR-378b, and decrease the fatty acid mitochondrial oxidation in obese rat livers by decreasing the protein expression of CPT1A and increasing the protein expression ratio of FAS/CPT1A.
authors.affiliation: Biology Center, China Institute of Sport Science, Beijing 100061, China; Sport Science Research Center of Shandong Province, Jinan 250102, China; Department of Exercise and Health Sciences, University of Massachusetts Boston, Boston, MA 02125, USA; School of Life Sciences, Shandong University, Jinan 250100, China.
authors.forename: Ying-li
authors.initials: YL
authors.lastname: Lu
authors.forename: Wen
authors.initials: W
authors.lastname: Jing
authors.forename: Lian-shi
authors.initials: LS
authors.lastname: Feng
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Jian-fang
authors.initials: JF
authors.lastname: Xu
authors.forename: Tong-jian
authors.initials: TJ
authors.lastname: You
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics / Q000378:metabolism; D059305:Diet, High-Fat; D054890:Fatty Acid Synthase, Type I / Q000235:genetics / Q000378:metabolism; D000860:Hypoxia / Q000235:genetics / Q000378:metabolism / Q000628:therapy; D050356:Lipid Metabolism / Q000235:genetics*; D008099:Liver / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D009765:Obesity / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D047493:PPAR alpha / Q000235:genetics / Q000378:metabolism; D010805:Physical Conditioning, Animal / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015431:Weight Loss
chemical_list: C572773:MIRN378 microRNA, rat; D035683:MicroRNAs; D047493:PPAR alpha; D002334:Carnitine O-Palmitoyltransferase; D054890:Fatty Acid Synthase, Type I
keywords: Hypoxic training; Lipid metabolism; Liver; MicroRNA; Obese rat