title: Structure and gene cluster of the O-antigen of Escherichia coli O68.
abstract: The O-polysaccharide (O-antigen) of Escherichia coli O68 was studied by sugar analysis, partial solvolysis with anhydrous trifluoroacetic acid, and 1D and 2D (1)H and (13)C NMR spectroscopies. The following structure of the branched heptasaccharide repeating unit was established: [structure: see text]. The O-antigen gene cluster of E. coli O68 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the O-antigen structure.
authors.affiliation: TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457, China; The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, China; Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, China.
authors.forename: Lingyan
authors.initials: L
authors.lastname: Jiang
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. Electronic address: perepel@ioc.ac.ru.
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Perepelov
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Filatov
authors.affiliation: The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, China; Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Sof'ya N
authors.initials: SN
authors.lastname: Senchenkova
authors.affiliation: The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, China; Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, China; State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D005810:Multigene Family; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics*; D016366:Open Reading Frames; D014269:Trifluoroacetic Acid / Q000737:chemistry
chemical_list: D019081:O Antigens; D014269:Trifluoroacetic Acid
keywords: Bacterial polysaccharide structure; Escherichia coli; Lipopolysaccharide; O-Antigen; O-Antigen gene cluster