title: Unraveling the complexity of lipid body organelles in human eosinophils.
abstract: Lipid-rich organelles are common in many cell types. In cells, such as adipocytes, these organelles are termed LDs, whereas in other cells, such as leukocytes, they are called LBs. The study of leukocyte LBs has attracted attention as a result of their association with human diseases. In leukocytes, such as eosinophils, LB accumulation has been documented extensively during inflammatory conditions. In these cells, LBs are linked to the regulation of immune responses by compartmentalization of several proteins and lipids involved in the control and biosynthesis of inflammatory mediators (eicosanoids). However, it has been unclear how diverse proteins, including membrane-associated enzymes involved in eicosanoid formation, incorporate into LBs, especially if the internal content of LBs is assumed to consist solely of stores of neutral lipids, as present within adipocyte LDs. Studies of the formation, function, and ultrastructure of LBs in eosinophils have been providing insights pertinent to LBs in other leukocytes. Here, we review current knowledge of the composition and function of leukocyte LBs as provided by studies of human eosinophil LBs, including recognitions of the internal architecture of eosinophil LBs based on 3D electron tomographic analyses.
authors.affiliation: Laboratory of Cellular Biology, Department of Biology, Institute of Biological Sciences, Federal University of Juiz de Fora, Brazil; and Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Rossana C N
authors.initials: RC
authors.lastname: Melo
authors.affiliation: Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA pweller@bidmc.harvard.edu.
authors.forename: Peter F
authors.initials: PF
authors.lastname: Weller
mesh_terms: D015415:Biomarkers; D004804:Eosinophils / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D007109:Immunity; D056747:Immunomodulation; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism; D066292:Lipid Droplets / Q000378:metabolism* / Q000648:ultrastructure; D050356:Lipid Metabolism; D014176:Protein Biosynthesis
chemical_list: D015415:Biomarkers
keywords: cell activation; eicosanoids; electron microscopy; immune responses; inflammation; lipid droplets