title: Applications of synchrotron-based spectroscopic techniques in studying nucleic acids and nucleic acid-functionalized nanomaterials.
abstract: In this review, we summarize recent progress in the application of synchrotron-based spectroscopic techniques for nucleic acid research that takes advantage of high-flux and high-brilliance electromagnetic radiation from synchrotron sources. The first section of the review focuses on the characterization of the structure and folding processes of nucleic acids using different types of synchrotron-based spectroscopies, such as X-ray absorption spectroscopy, X-ray emission spectroscopy, X-ray photoelectron spectroscopy, synchrotron radiation circular dichroism, X-ray footprinting and small-angle X-ray scattering. In the second section, the characterization of nucleic acid-based nanostructures, nucleic acid-functionalized nanomaterials and nucleic acid-lipid interactions using these spectroscopic techniques is summarized. Insights gained from these studies are described and future directions of this field are also discussed.
authors.affiliation: Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
authors.forename: Peiwen
authors.initials: P
authors.lastname: Wu
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Claire E
authors.initials: CE
authors.lastname: McGhee
authors.forename: Li Huey
authors.initials: LH
authors.lastname: Tan
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
mesh_terms: D002942:Circular Dichroism; D049329:Nanostructures / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D053838:Scattering, Small Angle; D013052:Spectrometry, X-Ray Emission; D017356:Synchrotrons; D056928:X-Ray Absorption Spectroscopy; D014961:X-Ray Diffraction
chemical_list: D009696:Nucleic Acids
keywords: SAXS; nanomaterials; nucleic acids; self-assembly; synchrotron