title: Cross-neutralizing antibody profile of Chinese HIV-1-infected individuals and the viral envelope features from elite neutralizers.
abstract: OBJECTIVE
  Knowledge about the profile of neutralization responses and the viral envelope features of HIV-1-infected individuals in China may provide new insights for vaccine design against local viruses.
  
  
  METHODS
  Eight hundred sixty plasma samples from antiretroviral treatment-naive HIV-1-infected individuals in Xinjiang province (611) and Guangxi province (249), who had acquired infection over 3 years through intravenous drug use or sexual contact, were examined for their ability to neutralize diverse envelope-pseudoviruses of 5 subtypes. The sequence features of the envelopes from elite neutralizers were analyzed.
  
  
  RESULTS
  From Xinjiang and Guangxi, 29.1% and 5.2% of plasmas displayed intrasubtype cross neutralization against subtype B and subtype C, respectively. From Xinjiang, 10.6% of the plasmas displayed broad neutralization against the 3 subtypes, B, C, and CRF01_AE; whereas only 2.4% from Guangxi displayed broad neutralization. Envelopes from 6 elite neutralizers were obtained by single-genome amplification. The variations of their envelopes including the lengths, glycans, and net charges in V1, V2, and V4 regions were compared with those from CRF07_BC env sequences from the HIV Sequence Database. The Envs from 3 elite neutralizers displayed the sensitivities to the monoclonal broadly neutralizing antibodies such as PG9, PG16, and 4E10. Some unique characteristics of the envelope glycoproteins from the Chinese elite neutralizers were found.
  
  
  CONCLUSIONS
  The neutralization profile of HIV-1-infected individuals in the 2 regions of China, where the HIV-1 subtypes are the representative in China, and the unique characteristics of the envelope glycoproteins from the Chinese elite neutralizers provide useful information for viral infection prevention and an insight for vaccine design against locally epidemic viruses.
authors.affiliation: *Department of Microbiology, Harbin Medical University, Heilongjiang Provincial Key Laboratory for Infection and Immunity, Key Laboratory of Etiology of Heilongjiang Province Education Bureau, Harbin, China; †Yili Prefecture Center for Disease Control and Prevention, Yili, China; ‡Department of Chemistry, Harbin Food and Drug Inspection Center, Harbin, China; §Guangxi Center for Disease Control and Prevention, Nanning, China; and ‖Departments of AIDS and STD, Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China.
authors.forename: Caiyun
authors.initials: C
authors.lastname: Ren
authors.forename: Song
authors.initials: S
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Zhuang
authors.forename: Haotong
authors.initials: H
authors.lastname: Yu
authors.forename: Jiaye
authors.initials: J
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Sun
authors.forename: Di
authors.initials: D
authors.lastname: Li
authors.forename: Haotian
authors.initials: H
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Shujia
authors.initials: S
authors.lastname: Liang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhong
authors.forename: Hong
authors.initials: H
authors.lastname: Ling
mesh_terms: D057134:Antibodies, Neutralizing / Q000097:blood*; D002681:China; D003429:Cross Reactions; D015483:HIV Antibodies / Q000097:blood*; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D054299:env Gene Products, Human Immunodeficiency Virus / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D015483:HIV Antibodies; D054299:env Gene Products, Human Immunodeficiency Virus