title: Development of microsatellite markers in the oil-producing species Vernicia fordii (Euphorbiaceae), a potential biodiesel feedstock.
abstract: OBJECTIVE
  Tung tree, Vernicia fordii, is native to China. Little has been done on genetics and breeding at the molecular level in this species, let alone utilizing microsatellite (simple sequence repeat [SSR]) markers. Therefore, a set of SSR molecular markers was developed for studies on molecular genetics and breeding in tung tree. •
  
  
  RESULTS
  We designed 78 SSR markers using a protocol based on the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol. Assessed in 81 V. fordii accessions, 40 of these markers were polymorphic and 12 of them showed monomorphism. When tested using six V. montana accessions, 52 of the markers were capable of PCR amplification and 25 were polymorphic. •
  
  
  CONCLUSIONS
  The newly developed SSR markers are effective and helpful in the evaluation of genetic germplasm and molecular breeding in tung tree.
authors.affiliation: Wuhan Botanical Garden and Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Chinese Academy of Sciences, Wuhan, Hubei 430074, People's Republic of China ; Graduate University of the Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Wuhan Botanical Garden and Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Chinese Academy of Sciences, Wuhan, Hubei 430074, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Pan
authors.affiliation: Wuhan Botanical Garden and Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Chinese Academy of Sciences, Wuhan, Hubei 430074, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Wuhan Botanical Garden and Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Chinese Academy of Sciences, Wuhan, Hubei 430074, People's Republic of China ; Graduate University of the Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, People's Republic of China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905 Israel.
authors.forename: Eviatar
authors.initials: E
authors.lastname: Nevo
authors.affiliation: Wuhan Botanical Garden and Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Chinese Academy of Sciences, Wuhan, Hubei 430074, People's Republic of China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Peng
keywords: Euphorbiaceae; FIASCO protocol; Vernicia fordii.; diversity evaluation; microsatellite marker