title: Effect of Calcium Alginate Microsphere Loaded With Vascular Endothelial Growth Factor on Adipose Tissue Transplantation.
abstract: Revascularization in the early period after transplantation is the key to improving adipocyte survival. Vascular endothelial growth factor (VEGF) is known as the master regulator of angiogenesis. However, consensus is lacking regarding safe and efficient methods for applying VEGF in free fat transplantation in the clinical setting. We constructed calcium alginate (CA) microspheres loaded with VEGF to increase the survival of implanted adipocytes. BALB/c nude mice were used as adipose tissue transplantation receptors. Adipocytes were mixed with CA microspheres loaded with VEGF and implanted subcutaneously into the dorsum of mice. Grafts were harvested at week 3, 6, and 12 after transplantation. We found that the mass and microvascular density of grafts in the VEGF+CA group (CA microspheres loaded with VEGF) were statistically higher than that of other groups in a time-dependent manner. We demonstrated that CA microspheres loaded with VEGF can significantly promote the fat graft neovascularization, thus improving adipocyte survival.
authors.affiliation: From the Department of Plastic Surgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Shi-Li
authors.initials: SL
authors.lastname: Ding
authors.forename: Meng-Yuan
authors.initials: MY
authors.lastname: Zhang
authors.forename: Song-Jia
authors.initials: SJ
authors.lastname: Tang
authors.forename: Hu
authors.initials: H
authors.lastname: Yang
authors.forename: Wei-Qiang
authors.initials: WQ
authors.lastname: Tan
mesh_terms: D000464:Alginates / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D004337:Drug Carriers; D005260:Female; D020723:Glucuronic Acid / Q000008:administration & dosage / Q000494:pharmacology; D006603:Hexuronic Acids / Q000008:administration & dosage / Q000494:pharmacology; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008863:Microspheres; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011897:Random Allocation; D019651:Plastic Surgery Procedures / Q000379:methods*; D050151:Subcutaneous Fat / Q000098:blood supply* / Q000187:drug effects / Q000637:transplantation*; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000464:Alginates; D004337:Drug Carriers; D006603:Hexuronic Acids; D042461:Vascular Endothelial Growth Factor A; D020723:Glucuronic Acid