title: Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the chicken CD8αα-BF2*0401 complex.
abstract: In the process of antigen presentation, the MHCI-CD8 complex is important for immune signal transduction by the activation of cytotoxic T cells. Here, the expression, purification, crystallization and X-ray analysis of the complex of the chicken MHC class I molecule BF2*0401 and CD8αα (CD8αα-BF2*0401) are reported. This complex was verified by SDS-PAGE analysis of a CD8αα-BF2*0401 crystal, which showed three bands corresponding to the molecular weights of BF2*0401, β2-microglobulin and CD8α, respectively. The crystal of CD8αα-BF2*0401 diffracted to 2.8 Å resolution and belonged to space group P21, with unit-cell parameters a = 90.6, b = 90.8, c = 94.9 Å, β = 98°. The Matthews coefficient and solvent content were calculated to be 2.88 Å(3) Da(-1) and ∼57.3%, respectively.
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
authors.forename: Mansoor
authors.initials: M
authors.lastname: Tariq
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
authors.forename: Chun
authors.initials: C
authors.lastname: Xia
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016827:CD8 Antigens / Q000737:chemistry* / Q000235:genetics; D002645:Chickens; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain; D017931:DNA Primers; D009842:Oligopeptides; C000595645:arginyl-tryptophyl-arginyl-leucyl-leucyl-leucyl-leucyl-lysyl-lysyl-histidine
keywords: BF2*0401; CD8αα; chicken