title: Selective binding and reverse transcription inhibition of single-strand poly(A) RNA by metal TMPyP complexes.
abstract: Ni-, Cu-, and Zn-TMPyP are capable of binding to single-strand poly(A) RNA with high preference and affinity and inhibiting the reverse transcription of RNA by both M-MuLV and HIV-1 reverse transcriptase. With 10 nM azidothymidine, the IC50 value of M-TMPyP could be lowered to 10(-1) μM order.
authors.affiliation: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University , Guangzhou 510275, P. R. China.
authors.forename: Zhu-Xin
authors.initials: ZX
authors.lastname: Zhou
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Xing
authors.initials: X
authors.lastname: Chen
authors.forename: Xiang-Jing
authors.initials: XJ
authors.lastname: Tian
authors.forename: Liang-Nian
authors.initials: LN
authors.lastname: Ji
mesh_terms: D001665:Binding Sites / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D019216:Metals, Heavy / Q000737:chemistry; D008968:Molecular Conformation; D008979:Moloney murine leukemia virus / Q000201:enzymology; D009942:Organometallic Compounds / Q000737:chemistry / Q000494:pharmacology*; D011061:Poly A / Q000037:antagonists & inhibitors* / Q000235:genetics; D011166:Porphyrins / Q000737:chemistry; D012367:RNA, Viral / Q000187:drug effects* / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D018894:Reverse Transcriptase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic / Q000187:drug effects* / Q000235:genetics
chemical_list: D019216:Metals, Heavy; D009942:Organometallic Compounds; D011166:Porphyrins; D012367:RNA, Viral; D018894:Reverse Transcriptase Inhibitors; C540459:meso-tetrakis(N-methylpyridinium-4-yl)porphyrin; D011061:Poly A; D012194:RNA-Directed DNA Polymerase