title: Follicle-stimulating hormone receptor gene polymorphism in chronic anovulatory women, with or without polycystic ovary syndrome: a cross-sectional study.
abstract: BACKGROUND
  Polymorphisms at codons 307 and 680 are the most commonly encountered allelic variants of the follicle-stimulating hormone receptor (FSHR) gene. Studies in Caucasians suggest that certain FSHR variants are more common in women with polycystic ovary syndrome (PCOS) than normal women. The objective of this study was to determine the distribution of FSHR gene polymorphisms at codons 307 and 680 in Thai women with chronic anovulation, without (121 women) and with PCOS (133 women), using 132 known fertile women as controls.
  
  
  METHODS
  DNA samples from peripheral blood lymphocytes were extracted and analyzed by polymerase chain reaction-restriction fragment length polymorphism.
  
  
  RESULTS
  The prevalence of Threonine307Threonine (TT), Threonine307Alanine (TA), and Alanine307Alanine (AA) genotypes at codon 307 was 53.0% (95% CI = 44.2-61.7%), 42.4% (95% CI = 34-51.3%), and 4.5% (95% CI = 1.9-10.1%) in controls; 52.6% (95% CI = 43.8-61.3%), 39.8% (95% CI = 31.6-48.7%), and 7.5% (95% CI = 3.9-13.7%) in PCOS women; and 50.4% (95% CI = 42.8-61.2%), 45.4% (95% CI = 34.9-53.1%), and 4.5% (95% CI = 1.5-9.6%) in anovulatory women without PCOS, respectively. The prevalence of Asparagine680Asparagine (NN), Asparagine680Serine (NS), and Serine680Serine (SS) genotypes at codon 680 was 54.5% (95% CI = 45.7-63.2%), 40.9% (95% CI = 32.5-49.8%), and 4.5% (95% CI = 1.9-10.1%) in controls; 51.9% (95% CI = 43.1-60.6%), 44.4% (95% CI = 35.8-53.2%), and 3.8% (95% CI = 1.4-9.0%) in PCOS women; and 47.9% (95% CI = 40.4-58.8%), 47.1% (95% CI = 36.5-54.7%), and 5.0% (95% CI = 2-10.9%) in anovulatory women without PCOS, respectively. The prevalence of FSHR gene polymorphisms at both codons were not statistically different among the three groups.
  
  
  CONCLUSIONS
  In Thai women, there was no association between the FSHR gene polymorphism at codons 307 and 680 and chronic anovulation.
authors.forename: Wanakan
authors.initials: W
authors.lastname: Singhasena
authors.forename: Tawiwan
authors.initials: T
authors.lastname: Pantasri
authors.forename: Waraporn
authors.initials: W
authors.lastname: Piromlertamorn
authors.forename: Sudarat
authors.initials: S
authors.lastname: Samchimchom
authors.affiliation: Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. tvutyava@med.cmu.ac.th.
authors.forename: Teraporn
authors.initials: T
authors.lastname: Vutyavanich
mesh_terms: D000328:Adult; D000483:Alleles; D019943:Amino Acid Substitution; D000858:Anovulation / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D003062:Codon; D003430:Cross-Sectional Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D006785:Hospitals, University; D006801:Humans; D008214:Lymphocytes; D010044:Outpatient Clinics, Hospital; D011085:Polycystic Ovary Syndrome / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011962:Receptors, FSH / Q000235:genetics* / Q000378:metabolism; D013785:Thailand; D055815:Young Adult
chemical_list: D003062:Codon; D011962:Receptors, FSH