title: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is associated with asymptomatic malaria in a rural community in Burkina Faso.
abstract: OBJECTIVE
  To investigate 4 combinations of mutations responsible for glucose-6-phosphate dehydrogenase (G6PD) deficiency in a rural community of Burkina Faso, a malaria endemic country.
  
  
  METHODS
  Two hundred individuals in a rural community were genotyped for the mutations A376G, G202A, A542T, G680T and T968C using TaqMan single nucleotide polymorphism assays and polymerase chain reaction followed by restriction fragment length polymorphism.
  
  
  RESULTS
  The prevalence of the G6PD deficiency was 9.5% in the study population. It was significantly higher in men compared to women (14.3% vs 6.0%, P=0.049). The 202A/376G G6PD A- was the only deficient variant detected. Plasmodium falciparum asymptomatic parasitaemia was significantly higher among the G6PD-non-deficient persons compared to the G6PD-deficient (P<0.001). The asymptomatic parasitaemia was also significantly higher among G6PD non-deficient compared to G6PD-heterozygous females (P<0.001).
  
  
  CONCLUSIONS
  This study showed that the G6PD A- variant associated with protection against asymptomatic malaria in Burkina Faso is probably the most common deficient variant.
authors.affiliation: Centre for Biomolecular Research Pietro Annigoni (CERBA) LABIOGENE UFR/SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso.
authors.forename: Abdoul Karim
authors.initials: AK
authors.lastname: Ouattara
authors.affiliation: Centre for Biomolecular Research Pietro Annigoni (CERBA) LABIOGENE UFR/SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso ; Laboratory of Molecular and Cellular Biology (LABMC), University of Science and Technology of Masuku (USTM), BP 943 Franceville, Gabon.
authors.forename: Cyrille
authors.initials: C
authors.lastname: Bisseye
authors.affiliation: Centre for Biomolecular Research Pietro Annigoni (CERBA) LABIOGENE UFR/SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso.
authors.forename: Bapio Valery Jean Télesphore Elvira
authors.initials: BV
authors.lastname: Bazie
authors.affiliation: Centre for Biomolecular Research Pietro Annigoni (CERBA) LABIOGENE UFR/SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso.
authors.forename: Birama
authors.initials: B
authors.lastname: Diarra
authors.affiliation: Centre for Biomolecular Research Pietro Annigoni (CERBA) LABIOGENE UFR/SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso.
authors.forename: Tegwindé Rebeca
authors.initials: TR
authors.lastname: Compaore
authors.affiliation: Centre for Biomolecular Research Pietro Annigoni (CERBA) LABIOGENE UFR/SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso.
authors.forename: Florencia
authors.initials: F
authors.lastname: Djigma
authors.affiliation: Centre for Biomolecular Research Pietro Annigoni (CERBA) LABIOGENE UFR/SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso.
authors.forename: Virginio
authors.initials: V
authors.lastname: Pietra
authors.affiliation: Centre for Biomolecular Research Pietro Annigoni (CERBA) LABIOGENE UFR/SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso.
authors.forename: Remy
authors.initials: R
authors.lastname: Moret
authors.affiliation: Centre for Biomolecular Research Pietro Annigoni (CERBA) LABIOGENE UFR/SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso.
authors.forename: Jacques
authors.initials: J
authors.lastname: Simpore
keywords: Asymptomatic malaria; Burkina Faso; Glucose-6-phosphate dehydrogenase deficiency; Mutations; Polymerase chain reaction