title: Component-resolved diagnosis in vespid venom-allergic individuals.
abstract: BACKGROUND
  Hymenoptera venom-allergic patients frequently present multiple sensitisations.
  
  
  OBJECTIVE
  To define the allergic profile by components in wasp allergic patients. To study the usefulness of specific IgE to components in cases of double sensitisation.
  
  
  METHODS
  Wasp allergic patients who needed Polistes and/or Vespula venom immunotherapy were included. Before immunotherapy and after two years of treatment the following specific IgE (sIgE) levels were measured: Apis mellifera, Vespula spp. Polistes spp., rVes v 5, rPol d 5, nVes v 5, nPol d 5, nVes v 1, nPol d 1, nApi m 1, nApi m 2 and peroxidase. Skin tests with venoms were performed. Based on the sIgE and the skin test results, Polistes and/or Vespula immunotherapy was administered.
  
  
  RESULTS
  Thirteen patients were included. Double sensitisation to Polistes/Vespula was detected in eight patients. Sensitisation to rVes v 5 and rPol d 5 was found in two of eight cases, to nVes v 1 and nPol d 1 in eight of 13 cases, and to nVes v 5 and nPol d 5 in 2 of 13 cases. Three patients received double immunotherapy with both wasps. One patient was treated with Vespula and nine with Polistes. sIgE levels decreased after two years of treatment. In patients who showed double sensitisation but were treated with only one venom, sIgE to both venoms decreased.
  
  
  CONCLUSIONS
  Components analysis can be useful to study double positivity. In case of doubt, double immunotherapy should be administered. Phospholipase was found to be a major allergen in our population.
authors.affiliation: Allergy Section, Hospital General Universitario de Ciudad Real, Ciudad Real, Spain. Electronic address: pagalindobonilla@gmail.com.
authors.forename: P A
authors.initials: PA
authors.lastname: Galindo-Bonilla
authors.affiliation: R&D, ALK-Abelló, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Galán-Nieto
authors.affiliation: Allergy Section, Hospital General Universitario de Ciudad Real, Ciudad Real, Spain.
authors.forename: T
authors.initials: T
authors.lastname: Alfaya-Arias
authors.affiliation: Allergy Section, Hospital General Universitario de Ciudad Real, Ciudad Real, Spain.
authors.forename: C
authors.initials: C
authors.lastname: García-Rodríguez
authors.affiliation: Allergy Section, Hospital General Universitario de Ciudad Real, Ciudad Real, Spain.
authors.forename: F
authors.initials: F
authors.lastname: de la Roca-Pinzón
authors.affiliation: Allergy Section, Hospital General Universitario de Ciudad Real, Ciudad Real, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Feo-Brito
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001514:Bee Venoms / Q000276:immunology; D001516:Bees / Q000276:immunology; D002648:Child; D003429:Cross Reactions / Q000276:immunology; D003888:Desensitization, Immunologic; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000276:immunology*; D007073:Immunoglobulin E; D008297:Male; D008875:Middle Aged; D054466:Phospholipases A1 / Q000276:immunology; D012882:Skin Tests; D014862:Wasp Venoms / Q000276:immunology*; D014863:Wasps / Q000276:immunology; D055815:Young Adult
chemical_list: D001514:Bee Venoms; D014862:Wasp Venoms; C040583:white-faced hornet venom antigen 5; D007073:Immunoglobulin E; D054466:Phospholipases A1; C080423:allergen Dol M I
keywords: Component-resolved diagnosis; Hymenoptera; Pol d 5; Ves v 5; Wasp venom allergy