title: Benefical effects of lycopene against contrast medium-induced oxidative stress, inflammation, autophagy, and apoptosis in rat kidney.
abstract: Currently, the number of imaging and interventional procedures that use contrast agents (CAs) is gradually increasing. Contrast-induced nephropathy (CIN) is the most important CA-related complication. Oxidative stress plays a significant role in its pathophysiology. Lycopene (LPN) is a natural substance with strong antioxidant capacity. The present study aimed to investigate the potential preventive effects of LPN against CIN. In total, 28 male Wistar albino rats were divided into 4 groups with 7 rats in each group; the groups include normal control group, LPN only group at a dose of 4 mg/kg/day for 10 days, CIN group by administering 10 mg/kg furosemide IM + 10 mg/kg indomethacin IP + 10 ml/kg iomeprol IV following 24-h dehydration, and CIN + LPN group. There were statistically significant increase in urea, creatinine, and malondialdehyde levels (p < 0.001, for all) but a significant decrease in glutathione, superoxide dismutase, catalase, and glutathione peroxidase levels (p < 0.001, for all) in the CIN group compared with the control group. On histological examination, a significant increase of infiltrated inflammatory cells and necrotic degenerative changes were observed in the CIN group and the immunohistochemical examination revealed a significant increase in inflammation (inducible nitric oxide synthase), autophagy (LC3/B), and apoptosis (cleaved caspase 3) in the CIN group compared with the control group (p < 0.05, for all). Significant improvements in these unfavorable parameters were observed with CIN + LPN group compared with the CIN only group. In conclusion, the favorable effects of LPN as an anti-inflammatory, antiautophagic, and antiapoptotic agent in an experimental model of CIN have been demonstrated.
authors.affiliation: Department of Cardiology, Erzincan University, Erzincan, Turkey mutlubuyuklu@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Buyuklu
authors.affiliation: Department of Biochemistry, Atatürk University, Erzurum, Turkey.
authors.forename: F M
authors.initials: FM
authors.lastname: Kandemir
authors.affiliation: Department of Pathology, Atatürk University, Erzurum, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Ozkaraca
authors.affiliation: Department of Family Physician, Atatürk University, Erzurum, Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Set
authors.affiliation: Department of Cardiology, Erzincan University, Erzincan, Turkey.
authors.forename: E M
authors.initials: EM
authors.lastname: Bakirci
authors.affiliation: Department of Cardiology, Erzincan University, Erzincan, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Topal
authors.affiliation: Department of Biochemistry, Atatürk University, Erzurum, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Ileriturk
authors.affiliation: Department of Nephrology, Erzincan University, Erzincan, Turkey.
authors.forename: K
authors.initials: K
authors.lastname: Turkmen
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D001343:Autophagy / Q000187:drug effects*; D002338:Carotenoids / Q000494:pharmacology / Q000627:therapeutic use*; D003287:Contrast Media / Q000633:toxicity*; D007150:Immunohistochemistry; D007668:Kidney / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D000077276:Lycopene; D008297:Male; D009393:Nephritis / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D052247:Nitric Oxide Synthase Type II / Q000276:immunology / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D003287:Contrast Media; D002338:Carotenoids; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D000077276:Lycopene
keywords: Contrast nephropathy; apoptosis; autophagy; inflammation; lycopene; oxidative stress