title: Simultaneous efficient removal of high-strength ammonia nitrogen and chemical oxygen demand from landfill leachate by using an extremely high ammonia nitrogen-resistant strain.
abstract: Bioaugmentation is a promising technology for pollutant elimination from stressed environments, and it would provide an efficient way to solve challenges in traditional biotreatment of wastewater with high strength of ammonia nitrogen (NH4(+)-N). A high NH4(+)-N-resistant bacteria strain, identified as Bacillus cereus (Jlu BC), was domesticated and isolated from the bacteria consortium in landfill leachate. Jlu BC could survive in 100 g/L NH4(+)-N environment, which indicated its extremely high NH4(+)-N tolerance than the stains found before. Jlu BC was employed in the bioaugmented system to remove high strength of NH4(+)-N from landfill leachate, and to increase the removal efficiency, response surface methodology (RSM) was used for optimizing bioaugmentation degradation conditions. At the optimum condition (initial pH 7.33, 4.14 days, initial chemical oxygen demand [COD] concentration [18,000 mg/L], 3.5 mL inoculated domesticated bacteria strain, 0.3 mg/mL phosphorus supplement, 30 °C, and 170 rpm), 94.74 ± 3.8% removal rate of NH4(+)-N was obtained, and the experiment data corresponded well with the predicted removal rate of the RSM models (95.50%). Furthermore, COD removal rate of 81.94 ± 1.4% was obtained simultaneously. The results presented are promising, and the screened strain would be of great practical importance in mature landfill leachate and other NH4(+)-N enrichment wastewater pollution control.
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, Changchun, 130012, People's Republic of China.
authors.forename: Dahai
authors.initials: D
authors.lastname: Yu
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, Changchun, 130012, People's Republic of China.
authors.forename: Jiyu
authors.initials: J
authors.lastname: Yang
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, Changchun, 130012, People's Republic of China.
authors.forename: Xuexun
authors.initials: X
authors.lastname: Fang
authors.affiliation: Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, Changchun, 130021, People's Republic of China.
authors.forename: Hejun
authors.initials: H
authors.lastname: Ren
mesh_terms: D000641:Ammonia / Q000302:isolation & purification*; D001409:Bacillus cereus / Q000502:physiology*; D001673:Biodegradation, Environmental; D057919:Biological Oxygen Demand Analysis; D001709:Biotechnology / Q000379:methods; D009584:Nitrogen / Q000302:isolation & purification*; D062065:Wastewater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
chemical_list: D000641:Ammonia; D009584:Nitrogen; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Bacillus cereus; ammonium nitrogen; bioaugmentation; landfill leachate; response surface methodology; tolerance