title: Revelation of staphylococcal cassette chromosome mec types in methicillin-resistant Staphylococcus aureus isolates from Thailand and Vietnam.
abstract: Methicillin resistant Staphylococcus aureus (MRSA) is highly prevalent, and its typing plays a crucial role in epidemiology and evolution in both health and community settings. Multiplex PCR and staphylococcal cassette chromosome mec (SCCmec) typing based on mec complexes and cassette chromosome recombinase (ccr) allotypes have been developed for MRSA identification. The first of these procedures can identify 4 mec classes (A, B, C1, and E) and 2 ccr allotypes (B2 and B4) in one tube, and the second can identify mecA, mec class C2, and 3 allotypes (A1, A3, and C). Our method offers a novel means to further differentiate between the main SCCmec types I through XI and is both highly sensitive (detectable up to 0.3ηg DNA) and specific (100%). Several SCCmec types (I, III, IV, V and a non-typeable group) were found in 66 MRSA isolates obtained from Ho Chi Minh City, Vietnam and Nakhon Pathom, Thailand. SCCmec type III was highly predominant in both regions. The designed assay is rapid, convenient, flexible, and reliable. Therefore, this assay is suitable for the high-throughput screening of the main SCCmec types of MRSA isolates.
authors.affiliation: Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand; Center of Medical Laboratory Services, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
authors.forename: R
authors.initials: R
authors.lastname: Lawung
authors.affiliation: Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand; Department of Microbiology, Faculty of Medicine, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam.
authors.forename: L V
authors.initials: LV
authors.lastname: Chuong
authors.affiliation: Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
authors.forename: R
authors.initials: R
authors.lastname: Cherdtrakulkiat
authors.affiliation: Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
authors.forename: A
authors.initials: A
authors.lastname: Srisarin
authors.affiliation: Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand. Electronic address: virapong.pra@mahidol.ac.th.
authors.forename: V
authors.initials: V
authors.lastname: Prachayasittikul
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013785:Thailand; D014744:Vietnam
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins
keywords: Methicillin resistant Staphylococcus aureus; SCCmec; ccr allotype; mec gene