title: FISH mapping for physical map improvement in the large genome of barley: a case study on chromosome 2H.
abstract: Fluorescence in situ hybridization (FISH) has been an efficient way for integrating physical and genetic maps of various small genomes like rice, sorghum and Brachypodium; whereas in the large genomes like barley, the repetitive nature of the genome complicates the generation and detection of single-copy FISH probes. Here, we used exemplarily physical map contigs of a defined interval of the long arm of barley chromosome 2H to evaluate the potential of FISH-based mapping as a supportive means for genetic anchoring of the physical map and to resolve the linear order of contigs along the respective chromosome. Repeat-free FISH probes corresponding to 8 previously anchored BAC contigs were specifically allocated to chromosome 2H. This represented an almost 90% success rate in single-copy FISH probe development. FISH mapping of contigs located in the subtelomeric region revealed an over-performance of genetic mapping over FISH for physical map anchoring.
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany.
authors.forename: Naser
authors.initials: N
authors.lastname: Poursarebani
authors.forename: Lu
authors.initials: L
authors.lastname: Ma
authors.forename: Thomas
authors.initials: T
authors.lastname: Schmutzer
authors.forename: Andreas
authors.initials: A
authors.lastname: Houben
authors.forename: Nils
authors.initials: N
authors.lastname: Stein
mesh_terms: D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant / Q000235:genetics*; D020451:Contig Mapping; D018744:DNA, Plant / Q000235:genetics; D018745:Genome, Plant; D001467:Hordeum / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008938:Mitosis; D026801:Synteny
chemical_list: D018744:DNA, Plant