title: Validation of the FluoroType® MRSA assay for the rapid identification of methicillin-resistant Staphylococcus aureus directly from patient material.
abstract: We performed the first evaluation study of the new HyBeacon based FluoroType(®) MRSA assay for the detection of MRSA directly from 617 patient specimens. Using culture as the reference method sensitivity and specificity were higher than 95%. Results were available within 2.5h, including DNA extraction.
authors.affiliation: Limbach Laboratory, Heidelberg, Germany. Electronic address: ulrich.eigner@labor-limbach.de.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Eigner
authors.affiliation: Limbach Laboratory, Heidelberg, Germany.
authors.forename: Anke
authors.initials: A
authors.lastname: Veldenzer
authors.affiliation: Limbach Laboratory, Heidelberg, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Holfelder
mesh_terms: D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology*
keywords: MRSA; PCR; Rapid test; Staphylococcus aureus; Validation