title: piggyBac transposon-derived targeting shRNA interference against the Bombyx mori nucleopolyhedrovirus (BmNPV).
abstract: The Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the most destructive diseases in silkworm, which has caused the main damage to sericulture industry. In this study, we developed a system of RNAi to prevent the BmNPV infection using the piggyBac transposon-derived targeting short hairpin RNA (shRNA) interference. The shRNAs targeting the genes of i.e.-1, lef-1, lef-2 and lef-3 of BmNPV were designed and used to inhibit the intracellular replication or multiplication of BmNPV in Bm cells. The highest activity was presented in the shRNA targeting the i.e.-1c of BmNPV, of which the inhibition rate reached 94.5 % in vitro. Further a stable Bm cell line of piggyBac transposon-derived targeting shRNA interference against BmNPV was established, which has a highly efficacious suppression on virus proliferation. These results indicated that the recombinant shRNA expression system was a useful tool for resistance to BmNPV in vitro. The approach by recombinant shRNAs opens a door of RNAi technology as a strategy that offering technically simpler, cheaper, and quicker gene knockdown for promising research and biotechnology application on silkworm lethal diseases.
authors.affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhou
authors.forename: Rui-ting
authors.initials: RT
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.forename: Shuang
authors.initials: S
authors.lastname: Liang
authors.forename: Mei-xian
authors.initials: MX
authors.lastname: Wang
authors.forename: Yun-gen
authors.initials: YG
authors.lastname: Miao
mesh_terms: D000818:Animals; D012831:Bombyx / Q000821:virology*; D004251:DNA Transposable Elements; D055785:Gene Knockdown Techniques / Q000379:methods*; D005814:Genes, Viral; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000502:physiology*; D034741:RNA, Small Interfering; D014779:Virus Replication
chemical_list: D004251:DNA Transposable Elements; D034741:RNA, Small Interfering