title: Advances in clinical applications of tissue proteomics: opportunities and challenges.
abstract: Mass spectrometry (MS)-based proteomics assays provide unprecedented opportunities for determination of protein expression in tissues for clinical applications. Within the last few years a number of clinical assays have been established. These include multiplexed selected reaction monitoring based approaches to quantify protein targets of therapeutic agents in cancer tissues and bottom-up shotgun MS-based proteomics approaches to diagnose and classify protein deposition disorders such as amyloidosis. These examples highlight the vast potential of MS-based proteomics as analytical and descriptive tools for clinical diagnosis of human disease. However, a number of major challenges remain for broader application and acceptance of such technologies in routine clinical care.
authors.affiliation: Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Dogan
mesh_terms: D000686:Amyloidosis / Q000175:diagnosis / Q000378:metabolism; D035843:Biomedical Research / Q000639:trends*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009369:Neoplasms / Q000175:diagnosis / Q000378:metabolism; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D020543:Proteome
keywords: amyloidosis; cancer; immunoglobulin; mass spectrometry; proteomics; transthyretin