title: Spatial co-localization of multi-enzymes by inorganic nanocrystal-protein complexes.
abstract: We report a simple precipitation method for the construction of spatially co-localized multi-enzyme systems based on inorganic nanocrystal-protein complexes. A spatially controlled multi-enzyme system exhibits enhanced overall catalytic performance, allowing for sensitive detection of glucose in solution.
authors.affiliation: Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China. junge@mail.tsinghua.edu.cn.
authors.forename: Zhixian
authors.initials: Z
authors.lastname: Li
authors.forename: Yifei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yechao
authors.initials: Y
authors.lastname: Su
authors.forename: Pingkai
authors.initials: P
authors.lastname: Ouyang
authors.forename: Jun
authors.initials: J
authors.lastname: Ge
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D012996:Solutions
chemical_list: D004800:Enzymes, Immobilized; D012996:Solutions; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D005947:Glucose