title: Effects of resveratrol on hydrogen peroxide-induced oxidative stress in embryonic neural stem cells.
abstract: Resveratrol, a natural phenolic compound, has been shown to prevent cardiovascular diseases and cancer and exhibit neuroprotective effects. In this study, we examined the neuroprotective and antioxidant effects of resveratrol against hydrogen peroxide in embryonic neural stem cells. Hydrogen peroxide treatment alone increased catalase and glutathione peroxidase activities but did not change superoxide dismutase levels compared with hydrogen peroxide + resveratrol treatment. Nitric oxide synthase activity and concomitant nitric oxide levels increased in response to hydrogen peroxide treatment. Conversely, resveratrol treatment decreased nitric oxide synthase activity and nitric oxide levels. Resveratrol also attenuated hydrogen peroxide-induced nuclear or mitochondrial DNA damage. We propose that resveratrol may be a promising agent for protecting embryonic neural stem cells because of its potential to decrease oxidative stress by inducing higher activity of antioxidant enzymes, decreasing nitric oxide production and nitric oxide synthase activity, and alleviating both nuclear and mitochondrial DNA damage.
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Ege University, Bornova-Izmir 35100, Turkey.
authors.forename: Sibel
authors.initials: S
authors.lastname: Konyalioglu
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Ege University, Bornova-Izmir 35100, Turkey.
authors.forename: Guliz
authors.initials: G
authors.lastname: Armagan
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Ege University, Bornova-Izmir 35100, Turkey.
authors.forename: Ayfer
authors.initials: A
authors.lastname: Yalcin
authors.affiliation: Department of Restorative Dentistry and Endodontics, Faculty of Dentistry, Ege University, Bornova-Izmir 35100, Turkey.
authors.forename: Cigdem
authors.initials: C
authors.lastname: Atalayin
authors.affiliation: Department of Physiology, School of Medicine, Ege University, Bornova-Izmir 35100, Turkey ; Center for Brain Research, Ege University, Bornova-Izmir 35100, Turkey.
authors.forename: Taner
authors.initials: T
authors.lastname: Dagci
keywords: DNA damage; catalase; embryonic neural stem cells; glutathione peroxidase; grants-supported paper; hydrogen peroxide; neural regeneration; neuroprotection; neuroregeneration; nitric oxide; nitric oxide synthase; resveratrol; stem cells; traditional Chinese medicine