title: Co(II)/Co(I) reduction-induced axial histidine-flipping in myoglobin reconstituted with a cobalt tetradehydrocorrin as a methionine synthase model.
abstract: A conjugate between apomyoglobin and cobalt tetradehydrocorrin was prepared to replicate the coordination behavior of cob(I)alamin in methionine synthase. X-ray crystallography reveals that the tetra-coordinated Co(I) species is formed through the cleavage of the axial Co-His93 ligation after the reduction of the penta-coordinated Co(II) cofactor in the heme pocket.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan. thayashi@chem.eng.osaka-u.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Hayashi
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Morita
authors.forename: Eiichi
authors.initials: E
authors.lastname: Mizohata
authors.forename: Koji
authors.initials: K
authors.lastname: Oohora
authors.forename: Jun
authors.initials: J
authors.lastname: Ohbayashi
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Hisaeda
mesh_terms: D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / Q000737:chemistry*; D000818:Animals; D001059:Apoproteins / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry / Q000201:enzymology*; D006639:Histidine / Q000737:chemistry*; D006736:Horses; D008958:Models, Molecular; D009211:Myoglobin / Q000737:chemistry*; D010084:Oxidation-Reduction; D014805:Vitamin B 12 / Q000737:chemistry*
chemical_list: D001059:Apoproteins; D009211:Myoglobin; C010857:apomyoglobin; D003035:Cobalt; D006639:Histidine; D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase; D014805:Vitamin B 12