title: Microarray-based DNA methylation study of Ewing's sarcoma of the bone.
abstract: Alterations in DNA methylation patterns are a hallmark of malignancy. However, the majority of epigenetic studies of Ewing's sarcoma have focused on the analysis of only a few candidate genes. Comprehensive studies are thus lacking and are required. The aim of the present study was to identify novel methylation markers in Ewing's sarcoma using microarray analysis. The current study reports the microarray-based DNA methylation study of 1,505 CpG sites of 807 cancer-related genes from 69 Ewing's sarcoma samples. The Illumina GoldenGate Methylation Cancer Panel I microarray was used, and with the appropriate controls (n=14), a total of 92 hypermethylated genes were identified in the Ewing's sarcoma samples. The majority of the hypermethylated genes were associated with cell adhesion, cell regulation, development and signal transduction. The overall methylation mean values were compared between patients who survived and those that did not. The overall methylation mean was significantly higher in the patients who did not survive (0.25±0.03) than in those who did (0.22±0.05) (P=0.0322). However, the overall methylation mean was not found to significantly correlate with age, gender or tumor location.GDF10,OSM,APCandHOXA11were the most significant differentially-methylated genes, however, their methylation levels were not found to significantly correlate with the survival rate. The DNA methylation profile of Ewing's sarcoma was characterized and 92 genes that were significantly hypermethylated were detected. A trend towards a more aggressive behavior was identified in the methylated group. The results of this study indicated that methylation may be significant in the development of Ewing's sarcoma.
authors.affiliation: Department of Pathology, College of Medicine, Hallym University, Anyang, Gyeonggi 431-070, Republic of Korea.
authors.forename: Hye-Rim
authors.initials: HR
authors.lastname: Park
authors.affiliation: Department of Biomedical Laboratory Science, College of Health Science, Cheongju University, Cheongju, Chungbuk 360-764, Republic of Korea.
authors.forename: Woon-Won
authors.initials: WW
authors.lastname: Jung
authors.affiliation: Department of Biomedical Laboratory Science, College of Health Science, Korea University, Seoul 136-703, Republic of Korea.
authors.forename: Hyun-Sook
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Pathology, College of Medicine, Kyung Hee University, Seoul 130-702, Republic of Korea.
authors.forename: Yong-Koo
authors.initials: YK
authors.lastname: Park
keywords: DNA methylation; Ewing’s sarcoma; microarray