title: Quantitative proteomics study of protective effects of grape seed procyanidin B2 on diabetic cardiomyopathy in db/db mice.
abstract: Diabetic cardiomyopathy is one of the major complications of diabetes mellitus. Oxidative stress appears to play a substantial role in cardiomyopathy. Grape seed procyanidin B2 (GSPB2) has been known as an anti-oxidant in treating diabetes mellitus; however, little is known about its effects and underlying mechanisms on diabetic cardiomyopathy. The present study is to explore the molecular targets of GSPB2 responsible for the anti-oxidative effects in db/db mice by quantitative proteomics. GSPB2 (30 mg/kg body weight/day) were intragastric administrated to db/db mice for 10 weeks. Proteomics of the heart tissue extracts by isobaric tags for relative and absolute quantification analysis was obtained from db/db mice. Our study provides important evidence that GSPB2 protect against cardiomyopathy in diabetes mellitus, which are believed to result from regulating the expression of key proteins involving cardiac fibrosis and proliferation. GSPB2 could be expected to become novel clinical application in fighting against diabetic cardiomyopathy.
authors.affiliation: a Key Laboratory of Cardiovascular Proteomics of Shandong Province, Department of Geriatric Endocrinology , Qi-Lu Hospital of Shandong University , Jinan , China.
authors.forename: Si-si
authors.initials: SS
authors.lastname: Luan
authors.forename: Fei
authors.initials: F
authors.lastname: Yu
authors.forename: Bao-ying
authors.initials: BY
authors.lastname: Li
authors.forename: Rui-jie
authors.initials: RJ
authors.lastname: Qin
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Cai
authors.forename: Wen-bin
authors.initials: WB
authors.lastname: Yin
authors.forename: Mei
authors.initials: M
authors.lastname: Cheng
authors.forename: Hai-qing
authors.initials: HQ
authors.lastname: Gao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D044946:Biflavonoids / Q000008:administration & dosage*; D002392:Catechin / Q000008:administration & dosage*; D058065:Diabetic Cardiomyopathies / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D005069:Evaluation Studies as Topic; D056604:Grape Seed Extract / Q000737:chemistry; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D044945:Proanthocyanidins / Q000008:administration & dosage*; D020011:Protective Agents / Q000008:administration & dosage / Q000737:chemistry; D040901:Proteomics
chemical_list: D000975:Antioxidants; D044946:Biflavonoids; D056604:Grape Seed Extract; D044945:Proanthocyanidins; D020011:Protective Agents; C479580:procyanidin B2; D002392:Catechin
keywords: Type-2 diabetes mellitus; cardiac fibrosis; cardiomyopathy; grape seed procyanidin B2; iTRAQ