title: One-step separation of antioxidant compounds from Erythrina variegata by high speed counter-current chromatography.
abstract: High speed counter-current chromatography (HSCCC) was used for separation and purification of antioxidant compounds from ethyl acetate fraction of the stem bark of Erythrina variegata. The optimal two-phase solvent system was composed of n-hexane-ethyl acetate-methanol-water (1:4:1:4, v/v/v/v). After one-step HSCCC separation, 75 mg of protocatechuic acid ( 1: ), 32 mg of chlorogenic acid ( 2: ) and 44 mg of caffeic acid ( 3: ) were purified from 420 mg of the ethyl acetate fraction. The purity of isolated compounds was determined up to 99.7% as determined by high-performance liquid chromatography (HPLC). The chemical structures of the three compounds were confirmed by UV, HPLC-MS/MS and (1)H NMR. The IC50 values of scavenging DPPH free radical for the three compounds were 22.5, 41.9 and 20.9 μg/mL, respectively. Protocatechuic acid and chlorogenic acid were obtained from the stem bark of E. variegata for the first time.
authors.affiliation: School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.affiliation: School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors.forename: Jingang
authors.initials: J
authors.lastname: Yu
authors.affiliation: School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Liao
authors.affiliation: School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors.forename: Peisen
authors.initials: P
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China Collaborative Innovation Center of Resource-Conserving & Environment-Friendly Society and Ecological Civilization, Changsha 410083, China xqchen@csu.edu.cn.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Chen