title: A simple and reliable liquid chromatography-tandem mass spectrometry method for the determination of aflatoxin B₁ in feed.
abstract: A chromatographic method is proposed for the analysis of aflatoxin B1 in cereal-based feed, particularly targeted to dairy animals. The method is based on a solid-liquid extraction followed by a Mycosep 226 clean-up. Accuracy and precision were established at the LOQ (1 µg kg⁻¹) with a spiked sample as well as with two other different naturally contaminated reference materials. The mean overall recovery (n = 18) was 100.8%, with a confidence interval of 2.7% and a CV% of 5.5%. The performance of the proposed method was compared with the AOAC method based on the use of immunoaffinity chromatography columns, proving that it could be considered a valid alternative. Moreover, the sample preparation is very simple and straightforward, potentially being applicable as a high-throughput method. On account of its simplicity and low cost, the method may be applied to the analysis of a large number of samples in the occasion of outbreaks of large-scale contamination.
authors.affiliation: a Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna , Brescia , Italy.
authors.forename: A
authors.initials: A
authors.lastname: Biancardi
authors.forename: C
authors.initials: C
authors.lastname: Dall'Asta
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis* / Q000737:chemistry; D053000:Analytic Sample Preparation Methods; D000821:Animal Feed / Q000032:analysis* / Q000592:standards; D002138:Calibration; D002274:Carcinogens, Environmental / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002523:Edible Grain / Q000737:chemistry*; D005062:European Union; D005506:Food Contamination; D005513:Food Inspection / Q000379:methods*; D007558:Italy; D057230:Limit of Detection; D015394:Molecular Structure; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D002274:Carcinogens, Environmental; D016604:Aflatoxin B1
keywords: cows; feeding stuffs; in-house validation; mass spectrometry; mycotoxins