title: [Down-regulation of TSPO expression doesn't affect the productions of TNF-α, IL-1β and IL-6 in LPS-stimulated BV-2 microglia].
abstract: OBJECTIVE
  To evaluate the effect of translocator protein (TSPO, Mr 18 000) on the productions of TNF-α, IL-1β and IL-6 in lipopolysaccharide (LPS)-stimulated BV-2 cells.
  
  
  METHODS
  RNA interference technique was used to decrease TSPO expression in BV-2 cells. Western blotting was performed to assess the level of TSPO protein in BV-2 cells transfected with TSPO siRNA. Then the mRNA and protein levels of TNF-α, IL-1β, and IL-6 were measured in LPS-stimulated BV-2 cells by real-time quantitative PCR (qRT-PCR) and ELISA, respectively.
  
  
  RESULTS
  The level of TSPO protein obviously decreased in BV-2 cells transfected with TSPO siRNA. However, knockdown of TSPO had no effect on the productions of TNF-α, IL-1β and IL-6 in LPS-stimulated BV-2 cells.
  
  
  CONCLUSIONS
  Down-regulated TSPO is not directly involved in regulating TNF-α, IL-1β and IL-6 productions induced by LPS in microglia.
authors.affiliation: Department of Nuclear Medicine, First Affiliated Hospital, Xiamen University, Xiamen 361003, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Dou
authors.affiliation: Department of Nuclear Medicine, First Affiliated Hospital, Xiamen University, Xiamen 361003, China.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Wenjiao
authors.initials: W
authors.lastname: Li
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Chundong
authors.initials: C
authors.lastname: Yu
authors.affiliation: Department of Nuclear Medicine, First Affiliated Hospital, Xiamen University, Xiamen 361003, China.
authors.forename: Xinhui
authors.initials: X
authors.lastname: Su
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D015536:Down-Regulation / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D053583:Interleukin-1beta / Q000235:genetics* / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D034622:RNA Interference; D018079:Receptors, GABA / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism
chemical_list: C463819:Bzrp protein, mouse; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D018079:Receptors, GABA; D014409:Tumor Necrosis Factor-alpha