title: Study of the T16189C variant and mitochondrial lineages in Tunisian and overall Mediterranean region.
abstract: The mitochondrial DNA (mtDNA) variant T16189C has been investigated in several metabolic diseases. In this study, we aimed to estimate the frequency of the T16189C variant in Tunisian and other Mediterranean populations and to evaluate the impact of this variant on the phylogeny of Mediterranean populations. Blood sample of 240 unrelated Tunisian subjects were recruited from several Tunisian localities. The hypervariable region 1 of the mtDNA were amplified and sequenced. Additional sequences (N = 4921) from Mediterranean populations were compiled from previous studies. The average frequency of T16189C variant in Tunisia (29%) is similar to that observed in North African and Near Eastern populations. Our findings showed positive correlation of the T16189C variant with Sub-Saharan and North African lineages, while a negative correlation was found with the Eurasian haplogroups, reaching its maximum with the Eurasian haplogroup H. The principal component analyses showed a high internal heterogeneity between Tunisian localities. At the Mediterranean scale, Tunisians are closer to North African (Algerian and Moroccan) and Near Eastern populations (Syrians and Palestinians) than to Europeans.
authors.affiliation: a Biomedical Genomics and Oncogenetics laboratory (LR 11 IPT 05), Institut Pasteur de Tunis, Université El Manar de Tunis , Tunis , Tunisia .
authors.forename: Sana
authors.initials: S
authors.lastname: Hsouna
authors.affiliation: a Biomedical Genomics and Oncogenetics laboratory (LR 11 IPT 05), Institut Pasteur de Tunis, Université El Manar de Tunis , Tunis , Tunisia .
authors.forename: Nizar
authors.initials: N
authors.lastname: Ben Halim
authors.affiliation: a Biomedical Genomics and Oncogenetics laboratory (LR 11 IPT 05), Institut Pasteur de Tunis, Université El Manar de Tunis , Tunis , Tunisia .
authors.forename: Khaled
authors.initials: K
authors.lastname: Lasram
authors.affiliation: b Laboratoire de Biochimie et Biologie Moléculaire , Faculté des Sciences et Techniques , Nouakchott , Mauritania .
authors.forename: Ghlana
authors.initials: G
authors.lastname: Meiloud
authors.affiliation: a Biomedical Genomics and Oncogenetics laboratory (LR 11 IPT 05), Institut Pasteur de Tunis, Université El Manar de Tunis , Tunis , Tunisia .
authors.forename: Imen
authors.initials: I
authors.lastname: Arfa
authors.affiliation: c Genetics Laboratory, Faculté de Médecine de Monastir , Monastir , Tunisia .
authors.forename: Emna
authors.initials: E
authors.lastname: Kerkeni
authors.affiliation: a Biomedical Genomics and Oncogenetics laboratory (LR 11 IPT 05), Institut Pasteur de Tunis, Université El Manar de Tunis , Tunis , Tunisia .
authors.forename: Lilia
authors.initials: L
authors.lastname: Romdhane
authors.affiliation: a Biomedical Genomics and Oncogenetics laboratory (LR 11 IPT 05), Institut Pasteur de Tunis, Université El Manar de Tunis , Tunis , Tunisia .
authors.forename: Henda
authors.initials: H
authors.lastname: Jamoussi
authors.affiliation: e Department of Biochemistry , Institut Pasteur de Tunis , Tunis , Tunisia , and.
authors.forename: Sonia
authors.initials: S
authors.lastname: Bahri
authors.affiliation: e Department of Biochemistry , Institut Pasteur de Tunis , Tunis , Tunisia , and.
authors.forename: Slim
authors.initials: S
authors.lastname: Ben Ammar
authors.affiliation: a Biomedical Genomics and Oncogenetics laboratory (LR 11 IPT 05), Institut Pasteur de Tunis, Université El Manar de Tunis , Tunis , Tunisia .|d Service de Consultation Externe et Exploration Fonctionnelle, Institut National de Nutrition , Tunis , Tunisia .
authors.forename: Abdelmajid
authors.initials: A
authors.lastname: Abid
authors.affiliation: f Laboratoire de Génétique Moléculaire Humaine, Département de Recherche Scientifique , Institut Pasteur du Maroc , Casablanca , Morocco.
authors.forename: Abdelhamid
authors.initials: A
authors.lastname: Barakat
authors.affiliation: b Laboratoire de Biochimie et Biologie Moléculaire , Faculté des Sciences et Techniques , Nouakchott , Mauritania .
authors.forename: Ahmed
authors.initials: A
authors.lastname: Houmeida
authors.affiliation: a Biomedical Genomics and Oncogenetics laboratory (LR 11 IPT 05), Institut Pasteur de Tunis, Université El Manar de Tunis , Tunis , Tunisia .
authors.forename: Sonia
authors.initials: S
authors.lastname: Abdelhak
authors.affiliation: a Biomedical Genomics and Oncogenetics laboratory (LR 11 IPT 05), Institut Pasteur de Tunis, Université El Manar de Tunis , Tunis , Tunisia .
authors.forename: Rym
authors.initials: R
authors.lastname: Kefi
mesh_terms: D004272:DNA, Mitochondrial / Q000737:chemistry*; D014644:Genetic Variation; D006239:Haplotypes; D006801:Humans; D019083:Mediterranean Region; D025341:Principal Component Analysis; D017422:Sequence Analysis, DNA; D014416:Tunisia
chemical_list: D004272:DNA, Mitochondrial
keywords: Mediterranenan populations; T16189C; Tunisia; mitochondrial haplogroups; principal component analysis