title: Association mapping for partial resistance to Phytophthora sojae in soybean (Glycine max (L.) Merr.).
abstract: Association mapping is a powerful high-resolution mapping tool for complex traits. The objective of this study was to identify QTLs for partial resistance to Phytophthora sojae. In this study, we evaluated a total of 214 soybean accessions by the hypocotyl inoculation method, and 175 were susceptible. The 175 susceptible accessions were then evaluated for P. sojae partial resistance using slant board assays. The 175 accessions were screened with 138 SSR markers that generated 730 SSR alleles. A subset of 495 SSR loci with minor allele frequency (MAF) ≥ 0.05 was used for association mapping by the Tassel general linear model (GLM) and mixed linear model (MLM) programmes. This soybean population could be divided into two subpopulations and no or weak relatedness was detected between pairwise accessions. Four SSR alleles, Satt634-133, Satt634-149, Sat_222-168 and Satt301-190, associated with partial resistance to P. sojae were detected by both GLM and MLM methods. Of these identified markers, one marker, Satt301, was located in regions where P. sojae resistance QTL have been previously mapped using linkage analysis. The identified markers will help to understand the genetic basis of partial resistance, and facilitate future marker-assistant selection aimed to improve resistance to P. sojae and reduce disease-related mortality in soybean.
authors.affiliation: National Center for Soybean Improvement / National Key Laboratory of Crop Genetics and Germplasm Enhancement / Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, People's Republic of China. hanx@njau.edu.cn.
authors.forename: Jutao
authors.initials: J
authors.lastname: Sun
authors.forename: Na
authors.initials: N
authors.lastname: Guo
authors.forename: Jun
authors.initials: J
authors.lastname: Lei
authors.forename: Lihong
authors.initials: L
authors.lastname: Li
authors.forename: Guanjun
authors.initials: G
authors.lastname: Hu
authors.forename: Han
authors.initials: H
authors.lastname: Xing
mesh_terms: D001947:Breeding; D002874:Chromosome Mapping; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D056726:Genetic Association Studies; D008040:Genetic Linkage; D005819:Genetic Markers; D014644:Genetic Variation; D054884:Host-Pathogen Interactions; D018895:Microsatellite Repeats; D010641:Phenotype; D010838:Phytophthora / Q000502:physiology*; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D013025:Glycine max / Q000235:genetics* / Q000276:immunology / Q000382:microbiology
chemical_list: D005819:Genetic Markers