title: Enantiomeric determination of four diastereoisomeric oxyneolignans from Bambusa tuldoides Munro.
abstract: BACKGROUND
  Bambusa tuldoides Munro, a bamboo species, is used as a health food, dietary supplement and folk medicine in China, and produces lignans that can be used to supplement other natural sources.
  
  
  OBJECTIVE
  To simultaneously separate eight stereoisomers of a particular type of oxyneolignan by chiral chromatography.
  
  
  METHODS
  Ninety-five per cent ethanol extracts of B. tuldoides Munro were analysed using HPLC/UV with a chiral column. The structures and configurations of isolated compounds were elucidated using NMR and circular dichroism (CD).
  
  
  RESULTS
  Four diastereoisomers were characterised and given the names oxyneolignans A, B, C and D. Furthermore, each oxyneolignan occurred as a pair of enantiomers. The oxyneolignans A-D consisted of the erythro-diastereoisomer of oxyneolignan at C7 and C8.
  
  
  CONCLUSIONS
  The chiral chromatography combined with the analysis techniques of NMR and CD reported here were reliable methods for discovering and separating the enantiomers.
authors.affiliation: State Forestry Administration Key Open Laboratory, International Centre for Bamboo and Rattan, Beijing, 100102, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Sun
authors.forename: Jin
authors.initials: J
authors.lastname: Yu
authors.forename: Pei-Cheng
authors.initials: PC
authors.lastname: Zhang
authors.forename: Yong-De
authors.initials: YD
authors.lastname: Yue
mesh_terms: D031723:Bambusa / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D000431:Ethanol; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism
chemical_list: D017705:Lignans; D010936:Plant Extracts; D000431:Ethanol
keywords: NMR; bamboo; circular dichroism; enantiomers; oxyneolignan