title: Active hexose correlated compound exerts therapeutic effects in lymphocyte driven colitis.
abstract: Active hexose correlated compound (AHCC) is a commercial extract of Basidiomycetes fungi enriched in oligosaccharides that is used as a human nutritional supplement for various purposes in humans. Our aim was to study the anti-inflammatory effect of AHCC in the CD4+ CD62L(+) T cell transfer model of colitis, considered one of the closest to the human disease. Colitis was induced by transfer of CD4(+) CD62L(+) T cells to recombination activating gene 1(-/-) mice. AHCC (75 mg/d) was administered by gavage as a post-treatment. Three groups were established: noncolitic, colitic (CD4(+) CD62L(+) transferred mice treated with vehicle), and AHCC (colitic treated with AHCC). AHCC improved colitis, as evidenced by a 24% lower colonic myeloperoxidase and a 21% lower alkaline phosphatase activity. In addition, a decreased secretion of proinflammatory genes assessed by RT-qPCR was observed, particularly TNF-α and IL-1β. Ex vivo mesenteric lymph node cells obtained from AHCC treated mice exhibited a fully normalized production of IL-6, IL-17, and IL-10 (p < 0.05). Also, AHCC treated mice exhibited decreased STAT4 and IκB-α phosphorylation in splenic CD4(+) cells. Our data provide validation of AHCC colonic anti-inflammatory activity in a chronic, T cell driven model of inflammatory bowel disease.
authors.affiliation: Departments of Pharmacology, CIBERehd, School of Pharmacy, Instituto de Investigación Biosanitaria ibs. GRANADA, University of Granada, Granada, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Mascaraque
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Suárez
authors.forename: Antonio
authors.initials: A
authors.lastname: Zarzuelo
authors.forename: Fermín
authors.initials: F
authors.lastname: Sánchez de Medina
authors.forename: Olga
authors.initials: O
authors.lastname: Martínez-Augustin
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D003092:Colitis / Q000188:drug therapy*; D003106:Colon / Q000187:drug effects / Q000473:pathology; D004195:Disease Models, Animal; D020672:I-kappa B Proteins / Q000235:genetics / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D051379:Mice; D000072000:NF-KappaB Inhibitor alpha; D010766:Phosphorylation; D011134:Polysaccharides / Q000494:pharmacology*; D015203:Reproducibility of Results; D050797:STAT4 Transcription Factor / Q000235:genetics / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D020672:I-kappa B Proteins; D020381:Interleukin-17; D053583:Interleukin-1beta; D015850:Interleukin-6; C000606204:NFKBIA protein, human; C000606205:Nfkbia protein, mouse; D011134:Polysaccharides; D050797:STAT4 Transcription Factor; C494091:Stat4 protein, mouse; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D000072000:NF-KappaB Inhibitor alpha; C499366:Active Hexose Correlated Compound
keywords: AHCC; CD4+ CD62L+ T cell; Inflammatory bowel disease; Nutraceutical; Prebiotic