title: Changes in compressed neurons from dogs with acute and severe cauda equina constrictions following intrathecal injection of brain-derived neurotrophic factor-conjugated polymer nanoparticles.
abstract: This study established a dog model of acute multiple cauda equina constriction by experimental constriction injury (48 hours) of the lumbosacral central processes in dorsal root ganglia neurons. The repair effect of intrathecal injection of brain-derived neurotrophic factor with 15 mg encapsulated biodegradable poly(lactide-co-glycolide) nanoparticles on this injury was then analyzed. Dorsal root ganglion cells (L7) of all experimental dogs were analyzed using hematoxylin-eosin staining and immunohistochemistry at 1, 2 and 4 weeks following model induction. Intrathecal injection of brain-derived neurotrophic factor can relieve degeneration and inflammation, and elevate the expression of brain-derived neurotrophic factor in sensory neurons of compressed dorsal root ganglion. Simultaneously, intrathecal injection of brain-derived neurotrophic factor obviously improved neurological function in the dog model of acute multiple cauda equina constriction. Results verified that sustained intraspinal delivery of brain-derived neurotrophic factor encapsulated in biodegradable nanoparticles promoted the repair of histomorphology and function of neurons within the dorsal root ganglia in dogs with acute and severe cauda equina syndrome.
authors.affiliation: Center of Trauma Repair and Reconstruction of Chinese PLA and Department of Orthopedics of the 98th Hospital of Chinese PLA, Huzhou 313000, Zhejiang Province, China.
authors.forename: Junming
authors.initials: J
authors.lastname: Tan
authors.affiliation: Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
authors.forename: Jiangang
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Shi
authors.affiliation: Department of Pathologic Laboratory of Chest Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pathologic Laboratory of Chest Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Center of Trauma Repair and Reconstruction of Chinese PLA and Department of Orthopedics of the 98th Hospital of Chinese PLA, Huzhou 313000, Zhejiang Province, China.
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Wang
authors.affiliation: Center of Trauma Repair and Reconstruction of Chinese PLA and Department of Orthopedics of the 98th Hospital of Chinese PLA, Huzhou 313000, Zhejiang Province, China.
authors.forename: Dechun
authors.initials: D
authors.lastname: Chen
authors.affiliation: Center of Trauma Repair and Reconstruction of Chinese PLA and Department of Orthopedics of the 98th Hospital of Chinese PLA, Huzhou 313000, Zhejiang Province, China.
authors.forename: Shunming
authors.initials: S
authors.lastname: Xing
authors.affiliation: Center of Trauma Repair and Reconstruction of Chinese PLA and Department of Orthopedics of the 98th Hospital of Chinese PLA, Huzhou 313000, Zhejiang Province, China.
authors.forename: Lianbing
authors.initials: L
authors.lastname: Shen
authors.affiliation: Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
authors.forename: Lianshun
authors.initials: L
authors.lastname: Jia
authors.affiliation: Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Ye
authors.affiliation: Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
authors.forename: Hailong
authors.initials: H
authors.lastname: He
authors.affiliation: Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
authors.forename: Jiashun
authors.initials: J
authors.lastname: Li
keywords: brain-derived neurotrophic factor; cauda equina syndrome; dorsal root ganglion; grants-supported paper; multiple cauda equina constrictions; neural protection; neural regeneration; neuroregeneration; neurotrophic factors; peripheral nerve injury; photographs-containing paper