title: Normobaric hyperoxia treatment prevents early alteration in dopamine level in mice striatum after fluid percussion injury: a biochemical approach.
abstract: Dopamine (DA) is one of the key neurotransmitters in the striatum, which is functionally important for a variety of cognitive and motor behaviours. It is known that the striatum is vulnerable to damage from traumatic brain injury (TBI). However, a therapeutic approach has not yet been established to treat TBI. Hence, the present work aimed to evaluate the ability of Normobaric hyperoxia treatment (NBOT) to recover dopaminergic neurons following a fluid percussion injury (FPI) as a TBI experimental animal model. To examine this, mice were divided into four groups: (i) Control, (ii) Sham, (iii) FPI and (iv) FPI+NBOT. Mice were anesthetized and surgically prepared for FPI in the striatum and immediate exposure to NBOT at various time points (3, 6, 12 and 24 h). Dopamine levels were then estimated post injury by utilizing a commercially available ELISA method specific to DA. We found that DA levels were significantly reduced at 3 h, but there was no reduction at 6, 12 and 24 h in FPI groups when compared to the control and sham groups. Subjects receiving NBOT showed consistent increased DA levels at each time point when compared with Sham and FPI groups. These results suggest that FPI may alter DA levels at the early post-TBI stages but not in later stages. While DA levels increased in 6, 12 and 24 h in the FPI groups, NBOT could be used to accelerate the prevention of early dopaminergic neuronal damage following FPI injury and improve DA levels consistently.
authors.affiliation: 1Center for Neuroscience Services and Research(P3Neuro), Universiti Sains Malaysia, Jalan Hospital Universiti Sains Malaysia, Kubang Kerian, Kota Bharu, Kelantan, Malaysia.
authors.forename: Sangu
authors.initials: S
authors.lastname: Muthuraju
authors.forename: Mohammad Rafiqul
authors.initials: MR
authors.lastname: Islam
authors.forename: Soumya
authors.initials: S
authors.lastname: Pati
authors.forename: Hasnan
authors.initials: H
authors.lastname: Jaafar
authors.forename: Jafri Malin
authors.initials: JM
authors.lastname: Abdullah
authors.forename: Kamaruddin Mohd
authors.initials: KM
authors.lastname: Yusoff
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000628:therapy*; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism*; D059290:Dopaminergic Neurons / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D017072:Neostriatum / Q000378:metabolism*; D010102:Oxygen Inhalation Therapy / Q000379:methods*
chemical_list: D004298:Dopamine
keywords: Fluid percussion injury; dopamine; normobaric hyperoxia treatment; striatum