title: Computational fluid dynamics-based design of a microfabricated cell capture device.
abstract: A microfluidic cell capture device was designed, fabricated, evaluated by numerical simulations and validated experimentally. The cell capture device was designed with a minimal footprint compartment comprising internal micropillars with the goal to obtain a compact, integrated bioanalytical system. The design of the device was accomplished by computational fluid dynamics (CFD) simulations. Various microdevice designs were rapidly prototyped in poly-dimethylsiloxane using conventional soft lithograpy technique applying micropatterned SU-8 epoxy based negative photoresist as moulding replica. The numerically modeled flow characteristics of the cell capture device were experimentally validated by tracing and microscopic recording the flow trajectories using yeast cells. Finally, we give some perspectives on how CFD modeling can be used in the early stage of microfluidics-based cell capture device development.
authors.affiliation: MTA-PE Translational Glycomics Research Group, University of Pannonia, Veszprem, Hungary CEITEC-Central European Institute of Technology, Brno, Czech Republic.
authors.forename: Gabor
authors.initials: G
authors.lastname: Jarvas
authors.affiliation: MTA-PE Translational Glycomics Research Group, University of Pannonia, Veszprem, Hungary Horvath Laboratory of Bioseparation Sciences, University of Debrecen, Debrecen, Hungary.
authors.forename: Marton
authors.initials: M
authors.lastname: Szigeti
authors.affiliation: MTA-PE Translational Glycomics Research Group, University of Pannonia, Veszprem, Hungary.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Hajba
authors.affiliation: Institute for Technical Physics and Materials Science, Research Centre for Natural Sciences-HAS, Budapest, Hungary.
authors.forename: Peter
authors.initials: P
authors.lastname: Furjes
authors.affiliation: MTA-PE Translational Glycomics Research Group, University of Pannonia, Veszprem, Hungary Horvath Laboratory of Bioseparation Sciences, University of Debrecen, Debrecen, Hungary a.guttman@neu.edu.
authors.forename: Andras
authors.initials: A
authors.lastname: Guttman
mesh_terms: D019295:Computational Biology / Q000295:instrumentation*; D004867:Equipment Design; D057446:Hydrodynamics; D044085:Microfluidics / Q000295:instrumentation*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*