title: Computational Identification of Dengue Virus MicroRNA-Like Structures and their Cellular Targets.
abstract: MicroRNAs (miRNAs) are small, noncoding RNA molecules that regulate transcriptional and posttranscriptional gene regulation of the cell. Experimental evidence shows that miRNAs have a direct role in different cellular processes, such as immune function, apoptosis, and tumorigenesis. In a viral infection context, miRNAs have been connected with the interplay between host and pathogen, occupying a major role in pathogenesis. While numerous viral miRNAs from DNA viruses have been identified, characterization of functional RNA virus-encoded miRNAs and their potential targets is still ongoing. Here, we used an in silico approach to analyze dengue Virus genome sequences. Pre-miRNAs were extracted through VMir software, and the identification of putative pre-miRNAs and mature miRNAs was accessed using Support Vector Machine web tools. The targets were scanned using miRanda software and functionally annotated using ClueGo. Via computational tools, eight putative miRNAs were found to hybridize with numerous targets of morphogenesis, differentiation, migration, and growth pathways that may play a major role in the interaction of the virus and its host. Future approaches will focus on experimental validation of their presence and target messenger RNA genes to further elucidate their biological functions in human and mosquito cells.
authors.affiliation: Molecular and Translational Medicine Group, University of Antioquia, Medellín, Antioquia, Colombia.
authors.forename: Maicol
authors.initials: M
authors.lastname: Ospina-Bedoya
authors.affiliation: Molecular and Translational Medicine Group, University of Antioquia, Medellín, Antioquia, Colombia.
authors.forename: Natalia
authors.initials: N
authors.lastname: Campillo-Pedroza
authors.affiliation: Molecular and Translational Medicine Group, University of Antioquia, Medellín, Antioquia, Colombia.
authors.forename: Juan P
authors.initials: JP
authors.lastname: Franco-Salazar
authors.affiliation: Molecular and Translational Medicine Group, University of Antioquia, Medellín, Antioquia, Colombia.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Gallego-Gómez
keywords: dengue virus; flavivirus; functional annotation; in silico screening; microRNA precursor; target prediction