title: Urinary signatures of Renal Cell Carcinoma investigated by peptidomic approaches.
abstract: Renal Cell Carcinoma (RCC) is typically asymptomatic and surgery usually increases patient's lifespan only for early stage tumours. Moreover, solid renal masses cannot be confidently differentiated from RCC. Therefore, markers to distinguish malignant kidney tumours and for their detection are needed. Two different peptide signatures were obtained by a MALDI-TOF profiling approach based on urine pre-purification by C8 magnetic beads. One cluster of 12 signals could differentiate malignant tumours (n = 137) from benign renal masses and controls (n = 153) with sensitivity of 76% and specificity of 87% in the validation set. A second cluster of 12 signals distinguished clear cell RCC (n = 118) from controls (n = 137) with sensitivity and specificity values of 84% and 91%, respectively. Most of the peptide signals used in the two models were observed at higher abundance in patient urines and could be identified as fragments of proteins involved in tumour pathogenesis and progression. Among them: the Meprin 1α with a pro-angiogenic activity, the Probable G-protein coupled receptor 162, belonging to the GPCRs family and known to be associated with several key functions in cancer, the Osteopontin that strongly correlates to tumour stages and invasiveness, the Phosphorylase b kinase regulatory subunit alpha and the SeCreted and TransMembrane protein 1.
authors.affiliation: Department of Health Science, University of Milano-Bicocca, Monza, Italy.
authors.forename: Clizia
authors.initials: C
authors.lastname: Chinello
authors.affiliation: Department of Health Science, University of Milano-Bicocca, Monza, Italy.
authors.forename: Marta
authors.initials: M
authors.lastname: Cazzaniga
authors.affiliation: Department of Health Science, University of Milano-Bicocca, Monza, Italy.
authors.forename: Gabriele
authors.initials: G
authors.lastname: De Sio
authors.affiliation: Department of Health Science, University of Milano-Bicocca, Monza, Italy.
authors.forename: Andrew James
authors.initials: AJ
authors.lastname: Smith
authors.affiliation: Department of Health Science, University of Milano-Bicocca, Monza, Italy.
authors.forename: Erica
authors.initials: E
authors.lastname: Gianazza
authors.affiliation: Department of Specialistic Surgical Sciences, Urology unit, Ospedale Maggiore Policlinico Foundation, Milano, Italy.
authors.forename: Angelica
authors.initials: A
authors.lastname: Grasso
authors.affiliation: Department of Specialistic Surgical Sciences, Urology unit, Ospedale Maggiore Policlinico Foundation, Milano, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Rocco
authors.affiliation: Department of Laboratory Medicine, Hospital of Desio, Desio, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Signorini
authors.affiliation: Department of Surgical Pathology, Cytology, Medical Genetics and Nephropathology, Azienda Ospedaliera San Gerardo, Monza, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Grasso
authors.affiliation: Department of Medicine, Surgery and Dental Sciences, Pathology Unit, Ospedale Maggiore Policlinico Foundation Milano, Italy.
authors.forename: Silvano
authors.initials: S
authors.lastname: Bosari
authors.affiliation: Department of Informatics, Systems and Communication, University of Milano-Bicocca, Milano, Italy.
authors.forename: Italo
authors.initials: I
authors.lastname: Zoppis
authors.affiliation: Mosaiques Diagnostics GmbH, Hannover, Germany.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Dakna
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Yuri E M
authors.initials: YE
authors.lastname: van der Burgt
authors.affiliation: Department of Informatics, Systems and Communication, University of Milano-Bicocca, Milano, Italy.
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Mauri
authors.affiliation: Department of Health Science, University of Milano-Bicocca, Monza, Italy.
authors.forename: Fulvio
authors.initials: F
authors.lastname: Magni
mesh_terms: D000328:Adult; D000368:Aged; D002292:Carcinoma, Renal Cell / Q000652:urine*; D016000:Cluster Analysis; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000652:urine*; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000652:urine*; D040901:Proteomics; D055815:Young Adult
chemical_list: D010455:Peptides