title: A high-throughput colourimetric method for the determination of pH in crystallization screens.
abstract: The crystallization of proteins is dependent on the careful control of numerous parameters, one of these being pH. The pH of crystallization is generally reported as that of the buffer; however, the true pH has been found to be as many as four pH units away. Measurement of pH with a meter is time-consuming and requires the reformatting of the crystallization solution. To overcome this, a high-throughput method for pH determination of buffered solutions has been developed with results comparable to those of a pH meter.
authors.affiliation: Department of Chemistry, University of York, York YO10 5DD, England.
authors.forename: Jobie
authors.initials: J
authors.lastname: Kirkwood
authors.affiliation: Department of Chemistry, University of York, York YO10 5DD, England.
authors.forename: Julie
authors.initials: J
authors.lastname: Wilson
authors.affiliation: Department of Computer Science, University of York, York YO10 5DD, England.
authors.forename: Simon
authors.initials: S
authors.lastname: O'Keefe
authors.affiliation: AstraZeneca, Alderley Park, Cheshire SK10 4TF, England.
authors.forename: David
authors.initials: D
authors.lastname: Hargreaves
mesh_terms: D003124:Colorimetry / Q000379:methods*; D003460:Crystallization; D057166:High-Throughput Screening Assays; D006863:Hydrogen-Ion Concentration; D013056:Spectrophotometry, Ultraviolet
keywords: absorbance; bromothymol blue; colourimetry; crystallization; indicator dye; pH; spectrophotometry