title: The novel amyloid-beta peptide aptamer inhibits intracellular amyloid-beta peptide toxicity.
abstract: Amyloid β peptide binding alcohol dehydrogenase (ABAD) decoy peptide (DP) can competitively antagonize binding of amyloid β peptide to ABAD and inhibit the cytotoxic effects of amyloid β peptide. Based on peptide aptamers, the present study inserted ABAD-DP into the disulfide bond of human thioredoxin (TRX) using molecular cloning technique to construct a fusion gene that can express the TRX1-ABAD-DP-TRX2 aptamer. Moreover, adeno-associated virus was used to allow its stable expression. Immunofluorescent staining revealed the co-expression of the transduced fusion gene TRX1-ABAD-DP-TRX2 and amyloid β peptide in NIH-3T3 cells, indicating that the TRX1-ABAD-DP-TRX2 aptamer can bind amyloid β peptide within cells. In addition, cell morphology and MTT results suggested that TRX1-ABAD-DP-TRX2 attenuated amyloid β peptide-induced SH-SY5Y cell injury and improved cell viability. These findings confirmed the possibility of constructing TRX-based peptide aptamer using ABAD-DP. Moreover, TRX1-ABAD-DP-TRX2 inhibited the cytotoxic effect of amyloid β peptide.
authors.affiliation: Department of Neurology, First Hospital of Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Neurology, First Hospital of Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Neurology, First Hospital of Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Mingyue
authors.initials: M
authors.lastname: Jia
authors.affiliation: Department of Neurosurgery, First Hospital of Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Chi
authors.initials: C
authors.lastname: Ma
authors.affiliation: Department of Neurology, First Hospital of Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Infection, First Hospital of Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Lihe
authors.initials: L
authors.lastname: Che
authors.affiliation: Department of Neurology, First Hospital of Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Neurology, First Hospital of Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Wu
keywords: Alzheimer's disease; amyloid β peptide; amyloid β peptide binding alcohol dehydrogenase; aptamer; decoy peptide; gene therapy; grants-supported paper; mitochondrial dysfunction; molecular cloning; neural regeneration; neurodegenerative disease; neuroregeneration; photographs-containing paper; thioredoxin