title: Potato chip intake increases ascorbic acid levels and decreases reactive oxygen species in SMP30/GNL knockout mouse tissues.
abstract: Potato chips (PC) contain abundant amounts of the free radical scavenger ascorbic acid (AA) due to the rapid dehydration of potato tubers (Solanum tuberosum) that occurs during frying. To evaluate the antioxidant activity of PC, this study examined reactive oxygen species (ROS) levels in tissues from SMP30/GNL knockout (KO) mice that cannot synthesize AA and determined AA and ROS levels after the animals were fed 20 and 10% PC diets for 7 weeks. Compared with AA-sufficient mice, AA-depleted SMP30/GNL KO mice showed high ROS levels in tissues. SMP30/GNL KO mice fed a PC diet showed high AA and low ROS levels in the brain, heart, lung, testis, soleus muscle, plantaris muscle, stomach, small intestine, large intestine, eyeball, and epididymal fat compared with AA-depleted mice. The data suggest that PC intake increases AA levels and enhances ROS scavenging activity in tissues of SMP30/GNL KO mice, which are a promising model for evaluating the antioxidant activity of foods.
authors.affiliation: Molecular Regulation of Aging, Tokyo Metropolitan Institute of Gerontology , 35-2 Sakae-cho, Itabashi-ku, Tokyo 173-0015, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Kondo
authors.forename: Rui
authors.initials: R
authors.lastname: Sakuma
authors.forename: Megumi
authors.initials: M
authors.lastname: Ichisawa
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Ishihara
authors.forename: Misako
authors.initials: M
authors.lastname: Kubo
authors.forename: Setsuko
authors.initials: S
authors.lastname: Handa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mugita
authors.forename: Naoki
authors.initials: N
authors.lastname: Maruyama
authors.forename: Hidenori
authors.initials: H
authors.lastname: Koga
authors.forename: Akihito
authors.initials: A
authors.lastname: Ishigami
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D035281:Plant Tubers / Q000737:chemistry / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D011198:Solanum tuberosum / Q000737:chemistry / Q000378:metabolism*; D013737:Testis / Q000378:metabolism
chemical_list: D002135:Calcium-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D017382:Reactive Oxygen Species; C513324:Rgn protein, mouse; D001205:Ascorbic Acid
keywords: antioxidant; bioavailability; mouse model; potato; vitamin C