title: Neuropeptide Y protects cerebral cortical neurons by regulating microglial immune function.
abstract: Neuropeptide Y has been shown to inhibit the immunological activity of reactive microglia in the rat cerebral cortex, to reduce N-methyl-D-aspartate current (I NMDA) in cortical neurons, and protect neurons. In this study, after primary cultured microglia from the cerebral cortex of rats were treated with lipopolysaccharide, interleukin-1β and tumor necrosis factor-α levels in the cell culture medium increased, and mRNA expression of these cytokines also increased. After primary cultured cortical neurons were incubated with the lipopolysaccharide-treated microglial conditioned medium, peak I NMDA in neurons increased. These effects of lipopolysaccharide were suppressed by neuropeptide Y. After addition of the neuropeptide Y Y1 receptor antagonist BIBP3226, the effects of neuropeptide Y completely disappeared. These results suggest that neuropeptide Y prevents excessive production of interleukin-1β and tumor necrosis factor-α by inhibiting microglial reactivity. This reduces I NMDA in rat cortical neurons, preventing excitotoxicity, thereby protecting neurons.
authors.affiliation: Graduate School, Hebei Medical University, Shijiazhuang, Hebei Province, China.
authors.forename: Qijun
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Functional Neurosurgery, Hebei General Hospital, Shijiazhuang, Hebei Province, China.
authors.forename: Changzheng
authors.initials: C
authors.lastname: Dong
authors.affiliation: Department of Functional Neurosurgery, Hebei General Hospital, Shijiazhuang, Hebei Province, China.
authors.forename: Wenling
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Third Hospital, Hebei Medical University, Shijiazhuang, Hebei Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Bu
authors.affiliation: Department of Functional Neurosurgery, Hebei General Hospital, Shijiazhuang, Hebei Province, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Wu
authors.affiliation: Graduate School, Hebei Medical University, Shijiazhuang, Hebei Province, China ; Department of Functional Neurosurgery, Hebei General Hospital, Shijiazhuang, Hebei Province, China.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Zhao
keywords: INMDA; immunological activity; interleukin-1β; microglia; nerve regeneration; neural regeneration; neuropeptide Y; tumor necrosis factor-α