title: Non-rigid multi-frame registration of cell nuclei in live cell fluorescence microscopy image data.
abstract: The analysis of the motion of subcellular particles in live cell microscopy images is essential for understanding biological processes within cells. For accurate quantification of the particle motion, compensation of the motion and deformation of the cell nucleus is required. We introduce a non-rigid multi-frame registration approach for live cell fluorescence microscopy image data. Compared to existing approaches using pairwise registration, our approach exploits information from multiple consecutive images simultaneously to improve the registration accuracy. We present three intensity-based variants of the multi-frame registration approach and we investigate two different temporal weighting schemes. The approach has been successfully applied to synthetic and live cell microscopy image sequences, and an experimental comparison with non-rigid pairwise registration has been carried out.
authors.affiliation: Dept. Bioinformatics and Functional Genomics, Biomedical Computer Vision Group, University of Heidelberg, BioQuant, IPMB, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 267, 69120 Heidelberg, Germany. Electronic address: marco.tektonidis@bioquant.uni-heidelberg.de.
authors.forename: Marco
authors.initials: M
authors.lastname: Tektonidis
authors.affiliation: Dept. Bioinformatics and Functional Genomics, Biomedical Computer Vision Group, University of Heidelberg, BioQuant, IPMB, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 267, 69120 Heidelberg, Germany.
authors.forename: Il-Han
authors.initials: IH
authors.lastname: Kim
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
authors.forename: Yi-Chun M
authors.initials: YC
authors.lastname: Chen
authors.affiliation: Dept. Bioinformatics and Functional Genomics, Biomedical Computer Vision Group, University of Heidelberg, BioQuant, IPMB, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 267, 69120 Heidelberg, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Eils
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
authors.forename: David L
authors.initials: DL
authors.lastname: Spector
authors.affiliation: Dept. Bioinformatics and Functional Genomics, Biomedical Computer Vision Group, University of Heidelberg, BioQuant, IPMB, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 267, 69120 Heidelberg, Germany. Electronic address: k.rohr@dkfz.de.
authors.forename: Karl
authors.initials: K
authors.lastname: Rohr
mesh_terms: D000465:Algorithms; D002467:Cell Nucleus / Q000648:ultrastructure*; D058948:Cell Tracking / Q000379:methods*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D018715:Microscopy, Video / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique
keywords: Biomedical image processing; Image sequence analysis; Microscopy; Registration