title: Acute and subacute oral toxicity study on the flavonoid rich fraction of Monodora tenuifolia seed in albino rats.
abstract: OBJECTIVE
  To investigate the effects of the flavonoid rich fraction of Monodora tenuifolia seed on the haematology, histopathology and liver profile of Wistar albino rats.
  
  
  METHODS
  Toxicity study was investigated on the flavonoid rich fraction of Monodora tenuifolia in rats administered different concentrations orally for 28 d using standard laboratory procedures.
  
  
  RESULTS
  The LD50 of the flavonoid rich fraction was found to be above 5 000 mg/kg body weight in mice observed for 48 h. After the Day 14, biochemical markers of liver injury such as serum alanine aminotransferase, and aspartate aminotransferase decreased significantly (P<0.05 at doses of 100 and 200 mg/kg body weight and P<0.01 at 400 mg/kg) while serum alkaline phosphatase increased non-significantly (P>0.05). There was non-significant (P>0.05) effect observed across the groups in the levels of serum total protein, albumin, globulin, urea and creatinine. The result of histological examination showed various degrees of peribiliary hepatitis after the Day 14 which fizzled out after the Day 28.
  
  
  CONCLUSIONS
  The result therefore suggests that the seed extract is potentially safe.
authors.affiliation: Department of Biochemistry, Faculty of Sciences, Imo State University Owerri, Imo State, Nigeria.
authors.forename: Raphael Chukwuma
authors.initials: RC
authors.lastname: Ekeanyanwu
authors.affiliation: Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria Nsukka, Enugu State, Nigeria.
authors.forename: Obioma Uzoma
authors.initials: OU
authors.lastname: Njoku
keywords: Biochemical parameters; Haematological parameters; Histology; Monodora tenuifolia; Toxicity