title: Differentiation of neurosphere-derived rat neural stem cells into oligodendrocyte-like cells by repressing PDGF-α and Olig2 with triiodothyronine.
abstract: One of the approaches for treating demyelination diseases is cytotherapy, and adult stem cells are potential sources. In this investigation, we tried to increase the yield of oligodendrocyte-like cells (OLCs) by inducing neural stem cells generated from BMSCs-derived neurospheres, which were used for deriving the neural stem cells (NSCs). The latter were induced into OLCs by heregulin, PDGF-AA, bFGF and triiodothyronine (T3). The BMSCs, NS, NSCs and OLCs were characterized by using immunocytochemistry for fibronectin, CD44, CD90, CD45, Oct-4, O4, Olig2, O1 and MBP markers. PDGF receptor α (PDGFR-α), Olig2 and MOG expression were evaluated by RT-PCR. The BMSCs expressed CD44, CD90, CD106 and Oct-4; the NSCs were immunoreactive to nestin and neurofilament 68. Incubation of the NSCs for 4 days with heregulin, PDGF-AA and bFGF resulted in their induction into oligodendrocyte progenitor-like cells (OPLCs), which immunoreacted to O4, Olig2 and O1, while Olig2 and PDGFR-α were detected by RT-PCR. Replacing heregulin, PDGF-AA and bFGF with T3 for 6 days resulted in repression of O4, O1, Olig2 and PDGFR-α. The OLCs were co-cultured with motoneurons resulted in induction of MOG and MBP, which were expressed in functional OLCs. The latter can be generated from BMSCs-derive NS with high yield.
authors.affiliation: Department of Anatomical Sciences, School of Medical Sciences, Tarbiat Modares University, P.O. Box 14155-4838, Tehran, Iran.
authors.forename: Hojjat-Allah
authors.initials: HA
authors.lastname: Abbaszadeh
authors.affiliation: Department of Anatomical Sciences, School of Medical Sciences, Tarbiat Modares University, P.O. Box 14155-4838, Tehran, Iran; Shefa Neurosciences Research Center, Khatam Al-Anbia Hospital, Tehran, Iran. Electronic address: takialtr@modares.ac.ir.
authors.forename: Taki
authors.initials: T
authors.lastname: Tiraihi
authors.affiliation: Department of Anatomy, Shahed University, Tehran, Iran.
authors.forename: AliReza
authors.initials: A
authors.lastname: Delshad
authors.affiliation: Department of genetics, School of Basic Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Saghedizadeh
authors.affiliation: Shefa Neurosciences Research Center, Khatam Al-Anbia Hospital, Tehran, Iran.
authors.forename: Taher
authors.initials: T
authors.lastname: Taheri
authors.affiliation: Shefa Neurosciences Research Center, Khatam Al-Anbia Hospital, Tehran, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Kazemi
authors.affiliation: Middle Euphrates Neuroscience Center, Kufa University,College of Medicine, Annajaf Al-Ashraf, Iraq.
authors.forename: Hayder K
authors.initials: HK
authors.lastname: Hassoun
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D018507:Gene Expression Regulation, Developmental; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D058953:Neural Stem Cells / Q000187:drug effects* / Q000378:metabolism; D020890:Neuregulin-1 / Q000378:metabolism; D000076104:Oligodendrocyte Transcription Factor 2; D009836:Oligodendroglia / Q000166:cytology / Q000187:drug effects; D010982:Platelet-Derived Growth Factor / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D013234:Stem Cells / Q000166:cytology; D014284:Triiodothyronine / Q000008:administration & dosage
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D009419:Nerve Tissue Proteins; D020890:Neuregulin-1; D000076104:Oligodendrocyte Transcription Factor 2; D010982:Platelet-Derived Growth Factor; D014284:Triiodothyronine; C522729:Olig2 protein, rat; C075278:platelet-derived growth factor A
keywords: BMSCs; Differentiation; Induction; NSCs; Oligodendrocyte like cells