title: Overexpression of cytoglobin gene inhibits hypoxic injury to SH-SY5Y neuroblastoma cells.
abstract: A plasmid for cytoglobin expression, pAcGFP1-C1-cytoglobin, was transfected into SH-SY5Y cells. Cobalt chloride was used to establish a model of hypoxia. Western blotting indicated that cytoglobin was overexpressed and there was low expression of hypoxia-inducible factor-1α in SH-SY5Y cells after transfection. Following cobalt chloride-induced hypoxia, cytoglobin and hypoxia-inducible factor-1α expression gradually increased in SH-SY5Y cells. Flow cytometry showed that with increasing duration of hypoxia, the proportion of normal cells significantly diminished in the transfected and non-transfected groups. The proportion of cells in the early stages of apoptosis increased. However, the proportion of apoptotic cells was significantly lower in the transfected group compared with the non-transfected group. These results demonstrate that cytoglobin and hypoxia-inducible factor-1α are strongly up-regulated by hypoxia, and that there is a strong relationship between hypoxia-inducible factor-1α and cytoglobin during hypoxic injury.
authors.affiliation: Department of Ophthalmology, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning Province, China.
authors.forename: Xiuling
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Ophthalmology, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning Province, China.
authors.forename: Dianwen
authors.initials: D
authors.lastname: Gao
keywords: SH-SY5Y cells; cobalt chloride; cytoglobin; green fluorescent protein; hypoxia; neural regeneration; neuroprotection; neuroregeneration; recombinant plasmid; transfection