title: Liquid chromatography-tandem mass spectrometry characterization of five new leucinostatins produced by Paecilomyces lilacinus CG-189.
abstract: The fungus Paecilomyces lilacinus produces leucinostatins—peptaibiotics that exert a range of biological activities including antimalarial, antiviral, antitumor and phytotoxicity. In this paper, we developed an analytical method employing LC-MS/MS in the precursor ion and product ion scan modes to elucidate five new leucinostatins. Direct Infusion (DI-MS) helped to identify the most abundant leucinostatins: F, D B2, S, A and K. MS/MS analysis using a triple quadrupole operating at different scan modes is a versatile tool to study natural products, especially peptaibiotics. Although DI-MS full-scan analysis is rapid and sensitive, it cannot distinguish between peptide isomers. On the other hand, LC-MS/MS operated in the precursor ion and product ion modes is time consuming, but allows identifying the structure of isomers or isobar in crude extracts.
authors.affiliation: Laboratory of Mass Spectrometry Applied to Natural Products, Department of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, Sao Paulo, Brazil.
authors.forename: Ana Flávia Canovas
authors.initials: AF
authors.lastname: Martinez
authors.affiliation: Laboratory of Mass Spectrometry Applied to Natural Products, Department of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, Sao Paulo, Brazil.
authors.forename: Luiz Alberto Beraldo
authors.initials: LA
authors.lastname: Moraes
mesh_terms: D023181:Antimicrobial Cationic Peptides; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009183:Mycotoxins / Q000737:chemistry*; D010143:Paecilomyces / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D023181:Antimicrobial Cationic Peptides; D009183:Mycotoxins; D010455:Peptides; C052006:leucinostatin D; C008268:leucinostatin A