title: Subunit composition of the human cytoplasmic dynein-2 complex.
abstract: Cytoplasmic dynein-2 is the motor for retrograde intraflagellar transport (IFT), and mutations in dynein-2 are known to cause skeletal ciliopathies. Here, we define for the first time the composition of the human cytoplasmic dynein-2 complex. We show that the proteins encoded by the ciliopathy genes WDR34 and WDR60 are bona fide dynein-2 intermediate chains and are both required for dynein-2 function. In addition, we identify TCTEX1D2 as a unique dynein-2 light chain that is itself required for cilia function. We define several subunits common to both dynein-1 and dynein-2, including TCTEX-1 (also known as DYNLT1) and TCTEX-3 (also known as DYNLT3), roadblock-1 (also known as DYNLRB1) and roadblock-2 (also known as DYNLRB2), and LC8-1 and LC8-2 light chains (DYNLL1 and DYNLL2, respectively). We also find that NudCD3 associates with dynein-2 as it does with dynein-1. By contrast, the common dynein-1 regulators dynactin, LIS1 (also known as PAFAH1B1) and BICD2 are not found in association with dynein-2. These data explain why mutations in either WDR34 or WDR60 cause disease, as well as identifying TCTEX1D2 as a candidate ciliopathy gene.
authors.affiliation: Cell Biology Laboratories, School of Biochemistry, Medical Sciences Building, University of Bristol, Bristol BS8 1TD, UK.
authors.forename: David
authors.initials: D
authors.lastname: Asante
authors.affiliation: Cell Biology Laboratories, School of Biochemistry, Medical Sciences Building, University of Bristol, Bristol BS8 1TD, UK.
authors.forename: Nicola L
authors.initials: NL
authors.lastname: Stevenson
authors.affiliation: Cell Biology Laboratories, School of Biochemistry, Medical Sciences Building, University of Bristol, Bristol BS8 1TD, UK david.stephens@bristol.ac.uk.
authors.forename: David J
authors.initials: DJ
authors.lastname: Stephens
mesh_terms: D001692:Biological Transport / Q000502:physiology; D002460:Cell Line; D002923:Cilia / Q000378:metabolism; D056445:Cytoplasmic Dyneins / Q000378:metabolism*; D004398:Dyneins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation
chemical_list: C533376:DYNLT1 protein, human; D056445:Cytoplasmic Dyneins; D004398:Dyneins
keywords: Cilia; Dynein; Intraflagellar transport; Microtubule motor