title: Actin-associated protein palladin is required for migration behavior and differentiation potential of C2C12 myoblast cells.
abstract: The actin-associated protein palladin has been shown to be involved in differentiation processes in non-muscle tissues. However, but its function in skeletal muscle has rarely been studied. Palladin plays important roles in the regulation of diverse actin-related signaling in a number of cell types. Since intact actin-cytoskeletal remodeling is necessary for myogenesis, in the present study, we pursue to investigate the role of actin-associated palladin in skeletal muscle differentiation. Palladin in C2C12 myoblasts is knocked-down using specific small interfering RNA (siRNA). The results show that down-regulation of palladin decreased migratory activity of mouse skeletal muscle C2C12 myoblasts. Furthermore, the depletion of palladin enhances C2C12 vitality and proliferation. Of note, the loss of palladin promotes C2C12 to express the myosin heavy chain, suggesting that palladin has a role in the modulation of C2C12 differentiation. It is thus proposed that palladin is required for normal C2C12 myogenesis in vitro.
authors.affiliation: Department of Life Sciences, National Cheng Kung University, No. 1, University Road, Tainan 70101, Taiwan, ROC.
authors.forename: Ngoc Uyen Nhi
authors.initials: NU
authors.lastname: Nguyen
authors.affiliation: Department of Life Sciences, National Cheng Kung University, No. 1, University Road, Tainan 70101, Taiwan, ROC.
authors.forename: Vincent Roderick
authors.initials: VR
authors.lastname: Liang
authors.affiliation: Department of Life Sciences, National Cheng Kung University, No. 1, University Road, Tainan 70101, Taiwan, ROC. Electronic address: hvwang@mail.ncku.edu.tw.
authors.forename: Hao-Ven
authors.initials: HV
authors.lastname: Wang
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000199:Actins / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D003598:Cytoskeletal Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D051379:Mice; D024510:Muscle Development / Q000235:genetics*; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins; D010750:Phosphoproteins; D034741:RNA, Small Interfering; C485873:palladin protein, mouse; D018995:Myosin Heavy Chains
keywords: C2C12; Differentiation; Migration; Myogenesis; Palladin; Proliferation