title: Successful live birth from oocytes after more than 14 years of cryopreservation.
abstract: OBJECTIVE
  To report a birth of a healthy girl after long-term oocyte cryopreservation by slow cooling in sodium depleted medium.
  
  
  METHODS
  Clinical application.
  
  
  METHODS
  University Affiliated, Private IVF center.
  
  
  METHODS
  A 38-year-old woman received embryos from IVF by intracytoplasmic sperm injection (ICSI) with her own oocytes that were cryopreserved by slow freezing in a low-sodium medium 14 years and 6 months before, when she was 24 years old.
  
  
  METHODS
  Survival, fertilization, cleavage, clinical pregnancy and delivery.
  
  
  RESULTS
  From six metaphase-II oocytes thawed, two survived, one was fertilized after ICSI and a cleaving embryo was transferred on day 3. A single term pregnancy was achieved, ending with the delivery of a healthy girl.
  
  
  CONCLUSIONS
  Cryopreservation after slow freezing in a sodium depleted medium maintained the developmental competence of oocytes after long-term storage and resulted in a successful live birth. As far as is known, this case represents, up to date, the longest storage period of cryopreserved human oocytes resulting in a live birth.
authors.affiliation: Halitus, Instituto Médico, Capital Federal, Buenos Aires, Argentina, fernanda.urquiza@halitus.com.
authors.forename: María Fernanda
authors.initials: MF
authors.lastname: Urquiza
authors.forename: Inés
authors.initials: I
authors.lastname: Carretero
authors.forename: Paola Rita
authors.initials: PR
authors.lastname: Cano Carabajal
authors.forename: Rodolfo Agustín
authors.initials: RA
authors.lastname: Pasqualini
authors.forename: María Macarena
authors.initials: MM
authors.lastname: Felici
authors.forename: Rodolfo Sergio
authors.initials: RS
authors.lastname: Pasqualini
authors.forename: Carlos José
authors.initials: CJ
authors.lastname: Quintans
mesh_terms: D000328:Adult; D015925:Cryopreservation / Q000379:methods; D005260:Female; D059247:Fertility Preservation / Q000379:methods*; D006801:Humans; D050498:Live Birth; D009865:Oocytes / Q000166:cytology* / Q000254:growth & development; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic; D013997:Time Factors