title: Pre-treatment with α-tocopherol and Terminalia arjuna ameliorates, pro-inflammatory cytokines, cardiac and apoptotic markers in myocardial infracted rats.
abstract: OBJECTIVE
  This study was aimed to evaluate the cardioprotective potential of combination of T. arjuna and α-tocopherol in isoproterenol induced myocardial injury.
  
  
  METHODS
  Wistar albino rats were pre-treated with hydroalcoholic extract of T. arjuna (HETA) and α-tocopherol (100 mg/kg b. w) daily for 30 days. Isoproterenol (ISP, 85 mg/kg b.w) was administered on 28th and 29th days at an interval of 24 hr.
  
  
  RESULTS
  ISP treated rats showed significant increase in lipid peroxidation (MDA), cardiac markers (CK-MB, SGOT, Trop I and LDH), pro-inflammatory cytokine (IL-6, CRP, TNF-α) levels and apoptotic markers (Bcl-2/Bax) as compared to healthy group. Pre-treatment with HETA 100 mg/kg b. w, reduced the elevated levels of these markers and significant effect (p<0.05) were observed with the combination of HETA and α-tocopherol at a dose of 100 mg/kg b. w, which was further confirmed by histopathological studies.
  
  
  CONCLUSIONS
  The present study concluded that the combination of α-tocopherol (100 mg/kg b. w) and hydroalcoholic extract of T. arjuna (100 mg/kg b. w) augments endogenous antioxidant compounds of rat heart and also prevents the myocardium from ISP-induced myocardial injury and it may have therapeutic and prophylactic value in the treatment of ischemic heart disease.
authors.forename: Santosh K
authors.initials: SK
authors.lastname: Shukla
authors.forename: Suman B
authors.initials: SB
authors.lastname: Sharma
authors.forename: Usha R
authors.initials: UR
authors.lastname: Singh
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002316:Cardiotonic Agents / Q000494:pharmacology; D002855:Chromatography, Thin Layer / Q000379:methods; D016207:Cytokines / Q000378:metabolism*; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D032342:Muscle Cells / Q000187:drug effects / Q000378:metabolism; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D017208:Rats, Wistar; D029766:Terminalia / Q000737:chemistry*; D024502:alpha-Tocopherol / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494828:Bax protein, rat; D002316:Cardiotonic Agents; D016207:Cytokines; D010936:Plant Extracts; D051028:bcl-2-Associated X Protein; D024502:alpha-Tocopherol; D007545:Isoproterenol
keywords: Isoproterenol; Myocardial infarction; Oxidative stress; Pro-inflammatory cytokines, Apoptosis; Terminalia arjuna