title: Comment on "Local impermeant anions establish the neuronal chloride concentration".
abstract: Glykys et al. (Reports, 7 February 2014, p. 670) proposed that cytoplasmic impermeant anions and polyanionic extracellular matrix glycoproteins establish the local neuronal intracellular chloride concentration, [Cl(-)]i, and thereby the polarity of γ-aminobutyric acid type A (GABAA) receptor signaling. The experimental procedures and results in this study are insufficient to support these conclusions. Contradictory results previously published by these authors and other laboratories are not referred to.
authors.affiliation: Institute of Physiology, University Medical Center, University of Mainz, Duesbergweg 6, D-55128 Mainz, Germany. luhmann@uni-mainz.de.
authors.forename: Heiko J
authors.initials: HJ
authors.lastname: Luhmann
authors.affiliation: Institute of Physiology, University Medical Center, University of Mainz, Duesbergweg 6, D-55128 Mainz, Germany.
authors.forename: Sergei
authors.initials: S
authors.lastname: Kirischuk
authors.affiliation: Institute of Physiology, University Medical Center, University of Mainz, Duesbergweg 6, D-55128 Mainz, Germany.
authors.forename: Werner
authors.initials: W
authors.lastname: Kilb
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D018118:Chloride Channels / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D011963:Receptors, GABA-A / Q000378:metabolism*
chemical_list: D018118:Chloride Channels; D002712:Chlorides; D011963:Receptors, GABA-A