title: Resident CD4+ T cells accumulate in lymphoid organs after prolonged antigen exposure.
abstract: Effector and memory CD4(+) T cells acquire distinct migratory properties depending on the type and location of the immune responses. Due to the highly dynamic nature of T cell circulation, the comprehensive analysis of these migratory routes requires dedicated experimental approaches. Here, we analyse the migration of effector/memory CD4(+) T cells by long-term in vivo cell tracking. We identify a resident population of antigen-experienced CD4(+) T cells that resides in lymph nodes and Peyer's patches without circulation or proliferation. Resident CD4(+) T cells constitute up to 50% of all effector/memory cells, including, but not limited to, follicular helper T cells. Furthermore, these non-circulating T cells possess a distinct T cell receptor repertoire and accumulate in Peyer's patches after continuous oral antigen exposure. Our results provide the first direct evidence for a resident population of effector/memory CD4(+) T cells that is retained in lymphoid tissues.
authors.affiliation: Institute of Immunology, Hannover Medical School, D-30625 Hannover, Germany.
authors.forename: Milas
authors.initials: M
authors.lastname: Ugur
authors.affiliation: Institute of Immunology, Hannover Medical School, D-30625 Hannover, Germany.
authors.forename: Olga
authors.initials: O
authors.lastname: Schulz
authors.affiliation: Institute of Biochemistry, Hannover Medical School, D-30625 Hannover, Germany.
authors.forename: Manoj B
authors.initials: MB
authors.lastname: Menon
authors.affiliation: Institute of Immunology, Hannover Medical School, D-30625 Hannover, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Krueger
authors.affiliation: 1] Institute of Immunology, Hannover Medical School, D-30625 Hannover, Germany [2] Institute of Molecular Medicine, RWTH Aachen University, D-52074 Aachen, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Pabst
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology*; D058948:Cell Tracking; D017931:DNA Primers / Q000235:genetics; D005434:Flow Cytometry; D006652:Histological Techniques; D006801:Humans; D008198:Lymph Nodes / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010581:Peyer's Patches / Q000166:cytology*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000941:Antigens; D017931:DNA Primers