title: Heterogeneous functions of perinatal mesenchymal stromal cells require a preselection before their banking for clinical use.
abstract: Perinatal sources of mesenchymal stromal cells (MSCs) have raised growing interest because they are readily and widely available with minimal ethical/legal issues and can easily be stored for allogeneic settings. In addition, perinatal tissues are known to be important in mediating the fetomaternal tolerance of pregnancy, which confer upon perinatal-MSCs (P-MSCs) a particular interest in immunomodulation. It has been recently shown that it is possible to deeply modify the secreted factor profiles of MSCs with different cytokine stimuli such as interferon gamma or tumor necrosis factor alpha to license MSCs for a better immunosuppresive potential. Therefore, we aimed to compare adult bone marrow-MSCs with MSCs from perinatal tissues (cord blood, umbilical cord, amnion, and chorion) on their in vitro immunological and stromacytic efficiencies under different priming conditions. Our results showed that P-MSCs had a potential to modulate the in vitro immune response and be useful for hematopoietic progenitor cell ex vivo expansion. However, we showed contrasted effects of cytokine priming embedded in an important between-donor variability. In conclusion, our study highlights the importance to elaborate predicitive in vitro tests to screen between-donor variability of perinatal tissues for banking allogeneic standardized MSCs.
authors.affiliation: 1 Unité de Thérapie Cellulaire et Réparation Tissulaire, Centre de Transfusion Sanguine des Armées "Jean Julliard", Institut de Recherche Biomédicale des Armées, Clamart , France .
authors.forename: Juliette
authors.initials: J
authors.lastname: Peltzer
authors.forename: Florent
authors.initials: F
authors.lastname: Montespan
authors.forename: Cédric
authors.initials: C
authors.lastname: Thepenier
authors.forename: Laetitia
authors.initials: L
authors.lastname: Boutin
authors.forename: Georges
authors.initials: G
authors.lastname: Uzan
authors.forename: Nathalie
authors.initials: N
authors.lastname: Rouas-Freiss
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Lataillade
mesh_terms: D050156:Adipogenesis; D000328:Adult; D064591:Allografts; D000650:Amnion / Q000166:cytology*; D001854:Bone Marrow Cells / Q000502:physiology; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D020219:Chondrogenesis; D002823:Chorion / Q000166:cytology*; D018920:Coculture Techniques; D003114:Colony-Forming Units Assay; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D056747:Immunomodulation; D016130:Immunophenotyping; D007231:Infant, Newborn; D007959:Lymphocyte Culture Test, Mixed; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D010012:Osteogenesis; D011247:Pregnancy; D014015:Tissue Banks; D014470:Umbilical Cord / Q000166:cytology*