title: Broad and direct interaction between TLR and Siglec families of pattern recognition receptors and its regulation by Neu1.
abstract: Both pathogen- and tissue damage-associated molecular patterns induce inflammation through toll-like receptors (TLRs), while sialic acid-binding immunoglobulin superfamily lectin receptors (Siglecs) provide negative regulation. Here we report extensive and direct interactions between these pattern recognition receptors. The promiscuous TLR binders were human SIGLEC-5/9 and mouse Siglec-3/E/F. Mouse Siglec-G did not show appreciable binding to any TLRs tested. Correspondingly, Siglece deletion enhanced dendritic cell responses to all microbial TLR ligands tested, while Siglecg deletion did not affect the responses to these ligands. TLR4 activation triggers Neu1 translocation to cell surface to disrupt TLR4:Siglec-E interaction. Conversely, sialidase inhibitor Neu5Gc2en prevented TLR4 ligand-induced disruption of TLR4:Siglec E/F interactions. Absence of Neu1 in hematopoietic cells or systematic treatment with sialidase inhibitor Neu5Gc2en protected mice against endotoxemia. Our data raised an intriguing possibility of a broad repression of TLR function by Siglecs and a sialidase-mediated de-repression that allows positive feedback of TLR activation during infection.
authors.affiliation: Center for Cancer and Immunology Research, Children's National Medical Center, Washington, DC, United States.
authors.forename: Guo-Yun
authors.initials: GY
authors.lastname: Chen
authors.affiliation: Center for Cancer and Immunology Research, Children's National Medical Center, Washington, DC, United States.
authors.forename: Nicholas K
authors.initials: NK
authors.lastname: Brown
authors.affiliation: Center for Cancer and Immunology Research, Children's National Medical Center, Washington, DC, United States.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Chemistry, University of California, Davis, Davis, United States.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Khedri
authors.affiliation: Department of Chemistry, University of California, Davis, Davis, United States.
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Chemistry, University of California, Davis, Davis, United States.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Genetics, St Jude Children's Research Hospital, Memphis, United States.
authors.forename: Diantha
authors.initials: D
authors.lastname: van de Vlekkert
authors.affiliation: Department of Genetics, St Jude Children's Research Hospital, Memphis, United States.
authors.forename: Alessandra
authors.initials: A
authors.lastname: D'Azzo
authors.affiliation: Center for Cancer and Immunology Research, Children's National Medical Center, Washington, DC, United States.
authors.forename: Pan
authors.initials: P
authors.lastname: Zheng
authors.affiliation: Center for Cancer and Immunology Research, Children's National Medical Center, Washington, DC, United States.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D019446:Endotoxemia / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D004791:Enzyme Inhibitors / Q000276:immunology / Q000494:pharmacology; D006801:Humans; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008856:Microscopy, Fluorescence; D009439:Neuraminidase / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000276:immunology / Q000494:pharmacology; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D051192:Receptors, Pattern Recognition / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D016207:Cytokines; D004791:Enzyme Inhibitors; D008070:Lipopolysaccharides; D009838:Oligodeoxyribonucleotides; D020033:Protein Isoforms; D051192:Receptors, Pattern Recognition; C422908:Siglece protein, mouse; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; C110128:Neu1 protein, mouse; D009439:Neuraminidase
keywords: E. coli; Siglec; immunology; mouse; sialidase; toll-like receptor