title: PET quantification of cerebral oxygen metabolism in small animals.
abstract: Understanding cerebral oxygen metabolism is of great importance in both clinical diagnosis and animal experiments because oxygen is a fundamental source of brain energy and supports brain functional activities. Since small animals such as rats are widely used to study various diseases including cerebral ischemia, cerebrovascular diseases, and neurodegenerative diseases, the development of a noninvasive in vivo measurement method of cerebral oxygen metabolic parameters such as oxygen extraction fraction (OEF) and cerebral metabolic rate of oxygen (CMRO2) as well as cerebral blood flow (CBF) and cerebral blood volume (CBV) has been a priority. Although positron emission tomography (PET) with (15)O labeled gas tracers has been recognized as a powerful way to evaluate cerebral oxygen metabolism in humans, this method could not be applied to rats due to technical problems and there were no reports of PET measurement of cerebral oxygen metabolism in rats until an (15)O-O2 injection method was developed a decade ago. Herein, we introduce an intravenous administration method using two types of injectable (15)O-O2 and an (15)O-O2 gas inhalation method through an airway placed in the trachea, which enables oxygen metabolism measurements in rats.
authors.affiliation: Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Temma
authors.affiliation: Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Koshino
authors.affiliation: Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan.
authors.forename: Tetsuaki
authors.initials: T
authors.lastname: Moriguchi
authors.affiliation: Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan.
authors.forename: Jun-ichiro
authors.initials: J
authors.lastname: Enmi
authors.affiliation: Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan.
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Iida
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001921:Brain / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D020520:Brain Infarction / Q000378:metabolism / Q000473:pathology; D002540:Cerebral Cortex / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D002560:Cerebrovascular Circulation; D008297:Male; D010100:Oxygen / Q000008:administration & dosage / Q000378:metabolism*; D010101:Oxygen Consumption; D010104:Oxygen Radioisotopes / Q000008:administration & dosage; D049268:Positron-Emission Tomography; D051381:Rats
chemical_list: D010104:Oxygen Radioisotopes; D010100:Oxygen