title: Simultaneous quantification of five steroid saponins from Dioscorea zingiberensis C.H. Wright in rat plasma by HPLC-MS/MS and its application to the pharmacokinetic studies.
abstract: A simple, reliable and sensitive high-performance liquid chromatography tandem mass spectrometry method (HPLC-MS/MS) was established for simultaneous analyses of the following 5 steroid saponins in rat plasma after the single dose administration of total steroid saponins extracted from the rhizome of Dioscorea zingiberensis C.H. Wright for the first time. Protodioscin, huangjiangsu A, zingiberensis new saponin, dioscin, and gracillin were quantified using ginsenoside Rb1 as the internal standard (IS). The plasma samples were pretreated by a single step acetonitrile-mediated protein precipitation. The chromatographic separation was performed on an Inersil ODS-3 C18 column (250mm×4.6mm, 5μm) with the mobile phase composed of acetonitrile and water containing 0.1% formic acid under a gradient elution mode at 0.2mLmin(-1) using a microsplit after the eluent from the HPLC apparatus. The quantification was accomplished on a triple quadrupole tandem mass spectrometer using the multiple reaction monitoring (MRM) in the positive ionization mode. The above five analytes were stable under sample storage and preparation conditions applied in the present study. The linearity, precision, accuracy, and recoveries of the analysis confirmed the requirements for quality-control purposes. After validation, this proposed method was successfully adopted to investigate the pharmacokinetic parameters of these five analytes.
authors.affiliation: Biomedicine Key Laboratory of Shaanxi Province, Northwest University, Xi'an 710069, China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Biomedicine Key Laboratory of Shaanxi Province, Northwest University, Xi'an 710069, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Laboratory of Bioseparation Technology, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA. Electronic address: itoy@nhlbi.nih.gov.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
authors.affiliation: Biomedicine Key Laboratory of Shaanxi Province, Northwest University, Xi'an 710069, China. Electronic address: guoxiaolizhang@163.com.
authors.forename: Wenji
authors.initials: W
authors.lastname: Sun
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001774:Blood Chemical Analysis; D002851:Chromatography, High Pressure Liquid; D027742:Dioscorea / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D057230:Limit of Detection; D008297:Male; D017207:Rats, Sprague-Dawley; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000008:administration & dosage / Q000493:pharmacokinetics*; D053719:Tandem Mass Spectrometry; D014018:Tissue Distribution
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins
keywords: Dioscorea zingiberensis C.H. Wright; HPLC–MS/MS; MRM; Pharmacokinetic parameters; Total steroid saponins