title: Primary culture of choroid plexuses from neonate rats containing progenitor cells capable of differentiation.
abstract: BACKGROUND
  The choroid plexuses, which could secrete a number of neurotrophins, have recently been used in transplantation in central nervous system diseases.
  
  
  OBJECTIVE
  To study the mechanism of nerve regeneration in the central nervous system by grafting choroid plexus tissues.
  
  
  METHODS
  Animal experimentation.
  
  
  METHODS
  The choroid plexuses from the lateral ventricles of neonatal rats were cultured in adherent culture, and immunocytochemical methods were used to analyse the progenitor cells on days 2, 6, and 10 after seeding.
  
  
  RESULTS
  Expression of both nestin and glial fibrillary acidic protein was observed in small cell aggregates on day 2 in primary culture. Most of the nestin-positive cells on day 6 were immunoreactive to glial fibrillary acidic protein antibody. No cells expressing nestin or glial fibrillary acidic protein were seen on day 10.
  
  
  CONCLUSIONS
  These experimental results indicate that the choroid plexus contains a specific cell population - progenitor cells. Under in vitro experimental conditions, the progenitor cells differentiated into choroid plexus epithelial cells but did not form neurons or astrocytes.
authors.affiliation: Department of Orthopaedics, the Second Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, China.
authors.forename: Sheng-Li
authors.initials: SL
authors.lastname: Huang
authors.affiliation: Department of Orthopaedics, the Second Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, China.
authors.forename: Xi-Jing
authors.initials: XJ
authors.lastname: He
authors.affiliation: Central Laboratory for Scientific Research, the Second Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, China.
authors.forename: Zong-Fang
authors.initials: ZF
authors.lastname: Li
authors.affiliation: Institute of Neurobiology, Xi'an Jiaotong University School of Medicine, Xi'an, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yao
authors.affiliation: Central Laboratory for Scientific Research, the Second Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, China.
authors.forename: Guo-Lian
authors.initials: GL
authors.lastname: Yuan
authors.affiliation: Department of Neurosurgery, the Second Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
keywords: Choroid plexus; differentiation; primary culture; progenitor cells; rat