title: Isolation and characterization of microsatellite loci in Byrsonima cydoniifolia (Malpighiaceae) and cross-amplification in B. crassifolia.
abstract: OBJECTIVE
  Microsatellite markers were developed and characterized in Byrsonima cydoniifolia (Malpighiaceae) to allow further investigation of genetic variation in natural populations. Cross-amplification was tested in the related species B. crassifolia. •
  
  
  RESULTS
  Seventeen microsatellite markers were isolated by a microsatellite-enriched library protocol. Fourteen polymorphic and three monomorphic loci were identified in B. cydoniifolia. The mean number of alleles in the three populations were 6.5, 6.5, and 8.2, ranging from three to 17 for different loci and populations. Mean observed and expected heterozygosities were 0.706 and 0.727, respectively. The fixation index was close to zero for all but two loci. Nine microsatellite loci were successfully cross-amplified in B. crassifolia. •
  
  
  CONCLUSIONS
  This new set of microsatellite markers will be a useful tool for genetic studies of B. cydoniifolia, supporting strategies for maintaining the genetic diversity of this species and possibly that of many related species.
authors.affiliation: Programa de Pós-graduação em Genética e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia CP 13174001-970, Goiás, Brazil ; Laboratório de Genética e Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia CP 13174001-970, Goiás, Brazil.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Bernardes
authors.affiliation: Laboratório de Genética e Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia CP 13174001-970, Goiás, Brazil.
authors.forename: Daniela Elaine
authors.initials: DE
authors.lastname: Dos Anjos
authors.affiliation: Laboratório de Genética e Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia CP 13174001-970, Goiás, Brazil.
authors.forename: Sara Giselle de Cássia Alexandre
authors.initials: SG
authors.lastname: Gondim
authors.affiliation: Instituto de Ciências Exatas e da Terra, Universidade Federal de Mato Grosso, Barra do Garças CP 78600-000, Mato Grosso, Brazil.
authors.forename: Devanir Mitsuyuki
authors.initials: DM
authors.lastname: Murakami
authors.affiliation: Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso, Barra do Garças CP 78600-000, Mato Grosso, Brazil.
authors.forename: Nair
authors.initials: N
authors.lastname: Bizão
authors.affiliation: Programa de Pós-graduação em Genética e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia CP 13174001-970, Goiás, Brazil ; Laboratório de Genética e Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia CP 13174001-970, Goiás, Brazil.
authors.forename: Mariana Pires de Campos
authors.initials: MP
authors.lastname: Telles
keywords: Byrsonima crassifolia; Byrsonima cydoniifolia; Malpighiaceae; genetic variability; murici; simple sequence repeat (SSR)