title: Self-aggregated nanoparticles of carboxylic curdlan-deoxycholic acid conjugates as a carrier of doxorubicin.
abstract: In this study, a new non-toxic, biodegradable, biocompatible and water-soluble carboxylic curdlan bearing the dissociable COOH group in 100% purity, which was prepared by 4-acetamido-TEMPO-mediated oxidation, was hydrophobically modified by deoxycholic acid (DOCA) to attain novel amphiphilic curdlan derivatives (CCDs) for the preparation of nano-carriers for antitumor drug doxorubicin (DOX). Under the effect of ultrasonication, the carboxylic curdlan derivatives in water were self-aggregated into spherical nanoparticles with diameters ranging from 214 nm to 380 nm. The critical aggregation concentrations decreased from 0.047 mg/mL to 0.016 mg/mL with increasing DS of DOCA. DOX-loaded CCD nanoparticles were prepared in an aqueous medium with dialysis method. The DOX-CCD nanoparticles exhibited pH- and dose-dependent drug release profiles during in vitro release experiments. Moreover, the drug transport mechanism was Fickian diffusion according to the Ritger-Peppas model. The CCD nanoparticles might be explored as potential carriers for hydrophobic drugs with controlled release and delivery functions.
authors.affiliation: The Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China; School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: jkyan_27@163.com.
authors.forename: Jing-Kun
authors.initials: JK
authors.lastname: Yan
authors.affiliation: School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Hai-Le
authors.initials: HL
authors.lastname: Ma
authors.affiliation: Department of Applied Biology & Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Juan-Juan
authors.initials: JJ
authors.lastname: Pei
authors.affiliation: School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Zhen-Bin
authors.initials: ZB
authors.lastname: Wang
authors.affiliation: Department of Applied Biology & Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong. Electronic address: jian-yong.wu@polyu.edu.hk.
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Wu
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry; D003840:Deoxycholic Acid / Q000008:administration & dosage / Q000737:chemistry*; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry; D004337:Drug Carriers; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D009369:Neoplasms / Q000188:drug therapy*; D047071:beta-Glucans / Q000008:administration & dosage / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D047071:beta-Glucans; D003840:Deoxycholic Acid; C038459:curdlan; D004317:Doxorubicin; D048271:Chitosan
keywords: Carboxylic curdlan; Deoxycholic acid; Self-aggregated nanoparticles