title: ACE inhibitory activity of pangasius catfish (Pangasius sutchi) skin and bone gelatin hydrolysate.
abstract: Skin and bone gelatins of pangasius catfish (Pangasius sutchi) were hydrolyzed with alcalase to isolate Angiotensin Converting Enzyme (ACE) inhibitory peptides. Samples with the highest degree of hydrolysis (DH) were separated into different fractions with molecular weight cut-off (MWCO) sizes of 10, 3 and 1 kDa, respectively and assayed for ACE inhibitory activity. Skin and bone gelatins had highest DH of 64.87 and 68.48 % after 2 and 1 h incubation, respectively. Results from this study indicated that by decreasing the molecular weight of fractions, ACE inhibitory activity was increased. Therefore, F3 permeates (MWCO < 1 kDa) of skin (IC50 = 3.2 μg/ml) and bone (IC50 = 1.3 μg/ml) gelatins possessed higher ACE inhibitory activity compared to their untreated gelatins and corresponding hydrolyzed fractions. In this study, the major amino acids were Glycine followed by Proline with an increased amount of hydrophobic amino acid content in F3 permeates of skin (4.01 %) and bone (5.79 %) gelatin. Digestion stability against gastrointestinal proteases did not show any remarkable change on ACE inhibition potency of these permeates. It was concluded that alcalase hydrolysis of P. sutchi by-products could be utilized as a part of functional food or ingredients of a formulated drug in order to control high blood pressure.
authors.affiliation: School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Malaysia ; Food and Drug Laboratory Research Center, Food and Drug Organization, MOH & ME, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mahmoodani
authors.affiliation: School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Malaysia.
authors.forename: Masomeh
authors.initials: M
authors.lastname: Ghassem
authors.affiliation: School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Malaysia.
authors.forename: Abdul Salam
authors.initials: AS
authors.lastname: Babji
authors.affiliation: School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Malaysia.
authors.forename: Salma Mohamad
authors.initials: SM
authors.lastname: Yusop
authors.affiliation: Food and Drug Laboratory Research Center, Food and Drug Organization, MOH & ME, Tehran, Iran.
authors.forename: Roya
authors.initials: R
authors.lastname: Khosrokhavar
keywords: ACE inhibitory; Enzymatic hydrolysis; Gelatin; High blood pressure; Pangasius sutchi