title: Production and optimization of L-asparaginase by an actinobacterium isolated from Nizampatnam mangrove ecosystem.
abstract: The aim of the present study was to isolate and screen actinomycetes from the mangrove sediments of Nizampatnam that are potent to produce L-asparaginase, an enzyme that catalyses the hydrolysis of asparagine. A total of 31 actinomycetes strains were isolated, of which 6 strains were positive for L-asparaginase. Several physico-chemical parameters were optimized for maximizing L-asparaginase production by the potent strain identified as Pseudonocardia endophytica VUK-10. Production of L-asparaginase by the strain was high in modified Asparagine glucose salts broth (FM-4)(3.96 IU/ml) as compared to other tested media. Maltose(6.99 IU ml(-1)) and L-asparagine (7.42 IU ml(-1)) were found to be the most suitable carbon and nitrogen sources for optimum enzyme production. Maximum production of L-asparaginase was found in the culture medium with pH 8 and temperature 30 degrees C incubated for four days. This is the first report on the production of L-asparaginase by Pseudonocardia endophytica VUK-10 from Nizampatnam mangrove sediments.
authors.forename: M Usha
authors.initials: MU
authors.lastname: Kiranmayi
authors.forename: Sudhakar
authors.initials: S
authors.lastname: Poda
authors.forename: M
authors.initials: M
authors.lastname: Vijayalakshmi
mesh_terms: D039903:Actinobacteria / Q000201:enzymology* / Q000302:isolation & purification; D001215:Asparaginase / Q000378:metabolism*; D007194:India; D053833:Wetlands
chemical_list: D001215:Asparaginase