title: Comparative evaluation of serum antioxidant levels in periodontally diseased patients: An interventional study.
abstract: BACKGROUND
  Periodontal disease is an immune-inflammatory disease characterized by connective tissue breakdown, loss of attachment and alveolar bone resorption. In normal physiology, there is a dynamic equilibrium between reactive oxygen species activity and antioxidant defense capacity and when that equilibrium shifts in favor of reactive oxygen species, oxidative stress results. Oxidative stress is thought to play a causative role in the pathogenesis of periodontal diseases. Catalase (CAT) protects cells from hydrogen peroxide generated within them. Even though, CAT is not essential for some cell types under normal conditions, it plays an important role countering the effects of oxidative stress on the cell.
  
  
  OBJECTIVE
  This study was designed to estimate and compare the CAT and total antioxidant capacity (TAOC) levels in the serum of periodontitis, gingivitis, and healthy individuals before and after nonsurgical periodontal therapy.
  
  
  METHODS
  This study was conducted in the Department of Periodontics, A. B. Shetty Memorial Institute of Dental Sciences, Deralakatte, Mangalore. The study was designed as a single blinded interventional study comprising of 75 subjects, inclusive of both sexes and divided into three groups of 25 patients each. Patients were categorized into chronic periodontitis, gingivitis and healthy. The severity of inflammation was assessed by using gingival index and pocket probing depth. Biochemical analysis was done to estimate the TAOC and CAT levels before and after nonsurgical periodontal therapy. Results obtained were then statistically analyzed using ANOVA test and paired t-test.
  
  
  RESULTS
  The results showed a higher level of serum TAOC and CAT in the healthy group compared with the other groups. The difference was found to be statistically significant (P < 0.0001). The posttreatment levels of TAOC were statistically higher than the pretreatment levels in periodontitis group.
authors.affiliation: Department of Periodontics, A. B. Shetty Institute of Dental Sciences, Derlakatte, Mangalore, India.
authors.forename: Biju
authors.initials: B
authors.lastname: Thomas
authors.affiliation: Department of Periodontics, A. B. Shetty Institute of Dental Sciences, Derlakatte, Mangalore, India.
authors.forename: Shabeer Mohamed
authors.initials: SM
authors.lastname: Madani
authors.affiliation: Department of Oral Surgery, A. B. Shetty Institute of Dental Sciences, Derlakatte, Mangalore, India.
authors.forename: B Rajendra
authors.initials: BR
authors.lastname: Prasad
authors.affiliation: Department of Biochemistry, K.S. Hegde Academy of Medical Sciences, Derlakatte, Mangalore, India.
authors.forename: Suchetha
authors.initials: S
authors.lastname: Kumari
keywords: Antioxidant defense; catalase; interventional study; periodontitis; total antioxidants