title: Ameliorative effect of alkaloid extract of Cyclea peltata (Poir.) Hook. f. & Thoms. roots (ACP) on APAP/CCl4 induced liver toxicity in Wistar rats and in vitro free radical scavenging property.
abstract: OBJECTIVE
  To evaluate the hepatoprotective and antioxidant properties of alkaloid extract of Cyclea peltata (C. peltata) against paracetamol/carbon tetra chloride induced liver damage in Wistar rats.
  
  
  METHODS
  In vivo paracetamol/carbon tetrachloride induced liver damage in Wistar rats, in vitro free radical scavenging studies, HPTLC estimation of tetrandrine and direct analysis in real time- mass spectrometry of alkaloid extract of C. peltata were used for the validation.
  
  
  RESULTS
  The results showed that pretreatment with alkaloid extract of C. peltata caused significant reduction of serum glutamate pyruvate transaminase, serum glutamate oxaloacetate transaminase, serum alkaline phosphatase, serum cholesterol, liver malondialdehyde levels. The reduced glutathione, catalase, superoxide dismutase levels in liver were increased with alkaloid extract of C. peltata treatment. These results were almost comparable to silymarin and normal control. Histopathological studies also substantiated the biochemical findings. The in vitro hydroxyl, superoxide and DPPH scavenging study of alkaloid extract of C. peltata showed significant free radical scavenging property.
  
  
  CONCLUSIONS
  The hepatoprotective property of alkaloid extract of C. peltata against paracetamol/carbon tetrachloride may be due the synergistic action of alkaloids especially tetrandrine, fangchinoline through free radical scavenging and thus preventing oxidative stress.
authors.affiliation: Division of Ethnomedicine and Ethnopharmacology, Jawaharlal Nehru Tropical Botanic Garden and Research Institute (JNTBGRI), Trivandrum, Kerala 695562, India.
authors.forename: Varghese Jancy
authors.initials: VJ
authors.lastname: Shine
authors.affiliation: Division of Ethnomedicine and Ethnopharmacology, Jawaharlal Nehru Tropical Botanic Garden and Research Institute (JNTBGRI), Trivandrum, Kerala 695562, India.
authors.forename: Panikamparambil Gopalakrishnan
authors.initials: PG
authors.lastname: Latha
authors.affiliation: Division of Ethnomedicine and Ethnopharmacology, Jawaharlal Nehru Tropical Botanic Garden and Research Institute (JNTBGRI), Trivandrum, Kerala 695562, India.
authors.forename: Somasekharan Nair Rajam
authors.initials: SN
authors.lastname: Suja
authors.affiliation: Division of Ethnomedicine and Ethnopharmacology, Jawaharlal Nehru Tropical Botanic Garden and Research Institute (JNTBGRI), Trivandrum, Kerala 695562, India.
authors.forename: Gangadharan Indira
authors.initials: GI
authors.lastname: Anuja
authors.affiliation: Deparment of Chemistry, Sree Narayana College, Punalur, Kollam-691305, Kerala, India.
authors.forename: Gopan
authors.initials: G
authors.lastname: Raj
authors.affiliation: Division of Ethnomedicine and Ethnopharmacology, Jawaharlal Nehru Tropical Botanic Garden and Research Institute (JNTBGRI), Trivandrum, Kerala 695562, India.
authors.forename: Sreedharan Nair
authors.initials: SN
authors.lastname: Rajasekharan
keywords: Catalase; Glutathione; Hydroxyl; Malondialdehyde; Superoxide dismutase; Tetrandrine