title: Bioconjugated iron oxide nanocubes: synthesis, functionalization, and vectorization.
abstract: A facile bottom-up approach for the synthesis of inorganic/organic bioconjugated nanoprobes based on iron oxide nanocubes as the core with a nanometric silica shell is demonstrated. Surface coating and functionalization protocols developed in this work offered good control over the shell thickness (8-40 nm) and enabled biovectorization of SiO2@Fe3O4 core-shell structures by covalent attachment of folic acid (FA) as a targeting unit for cellular uptake. The successful immobilization of folic acid was investigated both quantitatively (TGA, EA, XPS) and qualitatively (AT-IR, UV-vis, ζ-potential). Additionally, the magnetic behavior of the nanocomposites was monitored after each functionalization step. Cell viability studies confirmed low cytotoxicity of FA@SiO2@Fe3O4 conjugates, which makes them promising nanoprobes for targeted internalization by cells and their imaging.
authors.affiliation: Institute of Inorganic Chemistry, University of Cologne , Greinstrasse 6, Cologne 50939, Germany.
authors.forename: Laura
authors.initials: L
authors.lastname: Wortmann
authors.forename: Shaista
authors.initials: S
authors.lastname: Ilyas
authors.forename: Daniel
authors.initials: D
authors.lastname: Niznansky
authors.forename: Martin
authors.initials: M
authors.lastname: Valldor
authors.forename: Karim
authors.initials: K
authors.lastname: Arroub
authors.forename: Nadja
authors.initials: N
authors.lastname: Berger
authors.forename: Kamil
authors.initials: K
authors.lastname: Rahme
authors.forename: Justin
authors.initials: J
authors.lastname: Holmes
authors.forename: Sanjay
authors.initials: S
authors.lastname: Mathur
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry*; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005290:Ferric Compounds / Q000138:chemical synthesis* / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D060328:Magnetic Phenomena; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D056951:Photoelectron Spectroscopy; D012822:Silicon Dioxide / Q000737:chemistry; D015204:Spectroscopy, Mossbauer; D055672:Static Electricity; D013499:Surface Properties; D013818:Thermogravimetry
chemical_list: D001672:Biocompatible Materials; D005290:Ferric Compounds; C000499:ferric oxide; D012822:Silicon Dioxide
keywords: bioconjugated nanoprobes; cytotoxicity; folic acid; iron oxide-silica core−shell particles; magnetic properties