title: High quality draft genome sequence of Olivibacter sitiensis type strain (AW-6(T)), a diphenol degrader with genes involved in the catechol pathway.
abstract: Olivibacter sitiensis Ntougias et al. 2007 is a member of the family Sphingobacteriaceae, phylum Bacteroidetes. Members of the genus Olivibacter are phylogenetically diverse and of significant interest. They occur in diverse habitats, such as rhizosphere and contaminated soils, viscous wastes, composts, biofilter clean-up facilities on contaminated sites and cave environments, and they are involved in the degradation of complex and toxic compounds. Here we describe the features of O. sitiensis AW-6(T), together with the permanent-draft genome sequence and annotation. The organism was sequenced under the Genomic Encyclopedia for Bacteria and Archaea (GEBA) project at the DOE Joint Genome Institute and is the first genome sequence of a species within the genus Olivibacter. The genome is 5,053,571 bp long and is comprised of 110 scaffolds with an average GC content of 44.61%. Of the 4,565 genes predicted, 4,501 were protein-coding genes and 64 were RNA genes. Most protein-coding genes (68.52%) were assigned to a putative function. The identification of 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase-coding genes indicates involvement of this organism in the catechol catabolic pathway. In addition, genes encoding for β-1,4-xylanases and β-1,4-xylosidases reveal the xylanolytic action of O. sitiensis.
authors.affiliation: Democritus University of Thrace, Department of Environmental Engineering, Laboratory of Wastewater Management and Treatment Technologies, Xanthi, Greece.
authors.forename: Spyridon
authors.initials: S
authors.lastname: Ntougias
authors.affiliation: Department of Energy Joint Genome Institute, Genome Biology Program, Walnut Creek, CA, USA.
authors.forename: Alla
authors.initials: A
authors.lastname: Lapidus
authors.affiliation: Department of Energy Joint Genome Institute, Genome Biology Program, Walnut Creek, CA, USA.
authors.forename: James
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Energy Joint Genome Institute, Genome Biology Program, Walnut Creek, CA, USA.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Mavromatis
authors.affiliation: Department of Energy Joint Genome Institute, Genome Biology Program, Walnut Creek, CA, USA.
authors.forename: Amrita
authors.initials: A
authors.lastname: Pati
authors.affiliation: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Chen
authors.affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
authors.affiliation: Department of Energy Joint Genome Institute, Genome Biology Program, Walnut Creek, CA, USA.
authors.forename: Tanja
authors.initials: T
authors.lastname: Woyke
authors.affiliation: Agricultural University of Athens, Electron Microscopy Laboratory, Athens, Greece.
authors.forename: Constantinos
authors.initials: C
authors.lastname: Fasseas
authors.affiliation: Department of Energy Joint Genome Institute, Genome Biology Program, Walnut Creek, CA, USA ; King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Nikos C
authors.initials: NC
authors.lastname: Kyrpides
authors.affiliation: Agricultural University of Athens, Laboratory of General and Agricultural Microbiology, Athens, Greece.
authors.forename: Georgios I
authors.initials: GI
authors.lastname: Zervakis
keywords: Bacteroidetes; Sphingobacteriaceae; alkaline two-phase olive mill waste; hemicellulose degradation; β-1,4-xylanase; β-1,4-xylosidase