title: Oxidative stress and apoptosis in preeclampsia.
abstract: We aimed to determine the oxidative stress and antioxidant status in preeclamptic placenta. Also, we investigated the apoptotic index of villous trophoblast and proliferation index of cytotrophoblasts. The study included 32 pregnant with preeclampsia and 31 normotensive healthy pregnant women. Malondialdehyde (MDA) and total antioxidant status (TAS) levels were measured in the placenta. For detection of apoptosis and proliferation in trophoblast, apoptosis protease activating factor 1 (APAF-1) and Ki-67 were used. Placental MDA levels in preeclamptic women were significantly higher than normal pregnancies (p=0.002). There was no significant difference between the groups in the TAS levels of placenta (p=0.773). Also, the apoptotic index in villous trophoblasts increased (p<0.001), but proliferation index did not change in preeclampsia (p=0.850). Increased oxidative stress and apoptosis in pathological placenta are not balanced by antioxidant systems and proliferation mechanisms.
authors.affiliation: Bulent Ecevit University, Faculty of Medicine, Department of Biochemistry, Zonguldak, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Can
authors.affiliation: Bulent Ecevit University, Faculty of Medicine, Department of Biochemistry, Zonguldak, Turkey. Electronic address: berrak_guven@hotmail.com.
authors.forename: Berrak
authors.initials: B
authors.lastname: Guven
authors.affiliation: Bulent Ecevit University, Faculty of Medicine, Department of Pathology, Zonguldak, Turkey.
authors.forename: Sibel
authors.initials: S
authors.lastname: Bektas
authors.affiliation: Bulent Ecevit University, Faculty of Medicine, Department of Gynaecology and Obstetrics, Zonguldak, Turkey.
authors.forename: Ilker
authors.initials: I
authors.lastname: Arikan
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000235:genetics*; D053477:Apoptotic Protease-Activating Factor 1 / Q000097:blood; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000097:blood; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000235:genetics*; D010920:Placenta / Q000378:metabolism / Q000473:pathology; D011225:Pre-Eclampsia / Q000097:blood* / Q000235:genetics / Q000473:pathology; D011247:Pregnancy
chemical_list: C506592:APAF1 protein, human; D000975:Antioxidants; D053477:Apoptotic Protease-Activating Factor 1; D019394:Ki-67 Antigen; D008315:Malondialdehyde
keywords: APAF-1; Ki-67; Oxidative stress; Preeclampsia