title: Recognition of concanavalin A by cationic glucosylated liposomes.
abstract: The specificity of carbohydrate-lectin interaction has been reported as an attractive strategy for drug delivery in cancer therapy because of the high levels of lectins in several human malignancies. A novel cationic glucosylated amphiphile was therefore synthesized, as a model system, to attribute specificity toward d-glucose receptors to liposome formulations. Fluorescence experiments demonstrated that the monomeric glucosylated amphiphile is capable of interacting with fluorescently labeled concanavalin A, a D-glucose specific plant lectin. The interaction of concanavalin A with liposomes composed of a phospholipid and the glucosylated amphiphile was demonstrated by agglutination observed by optical density and dynamic laser light scattering measurements, thus paving the way to the preparation of other glycosilated amphiphiles differing for the length of polyoxyethylenic spacer, the sugar moieties, and/or the length of the hydrophobic chain.
authors.affiliation: Dipartimento di Chimica, Università degli Studi di Roma "Sapienza" , P.le A. Moro 5, 00185 Roma, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Mauceri
authors.forename: Stefano
authors.initials: S
authors.lastname: Borocci
authors.forename: Luciano
authors.initials: L
authors.lastname: Galantini
authors.forename: Luisa
authors.initials: L
authors.lastname: Giansanti
authors.forename: Giovanna
authors.initials: G
authors.lastname: Mancini
authors.forename: Antonio
authors.initials: A
authors.lastname: Martino
authors.forename: Livia
authors.initials: L
authors.lastname: Salvati Manni
authors.forename: Claudio
authors.initials: C
authors.lastname: Sperduto
mesh_terms: D002412:Cations / Q000138:chemical synthesis / Q000737:chemistry; D003208:Concanavalin A / Q000737:chemistry*; D006031:Glycosylation; D008081:Liposomes / Q000138:chemical synthesis / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D013501:Surface-Active Agents / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D002412:Cations; D008081:Liposomes; D013501:Surface-Active Agents; D003208:Concanavalin A