title: Formation peculiarities of radiation-induced aberrations of chromosomes in human cells under the modifying influence of chemical agents (comparative aspects).
abstract: OBJECTIVE
  The study objective was to determine and provide a comparative analysis of frequency and spectrum of the induced aberrations of chromosomes in culture of the human peripheral blood lymphocytes under the combined impact of radiation, co-mutagen, and chemical mutagen.
  
  
  METHODS
  Culture of human peripheral blood lymphocytes and cytogenetic methods have been used.
  
  
  RESULTS
  A co-mutagenic effect of the drug verapamil was established under the testing γ-irradiation of human peripheral blood lymphocytes in the dose range of 0.3-2.0 Gy at the expense of increased frequency of chromosomal aberrations (dicentrics). The combined effect of γ-irradiation and S-Nitrosoglutathione is directed on the induction and storage of chemical markers of exposure - the chromatid-type aberrations.
  
  
  CONCLUSIONS
  A co-mutagenic effect of verapamil under the low-dose γ-irradiation as a 2-fold increase of the chromosome-type aberrations (radiation markers) incidence was revealed at a chromosomal level in human peripheral blood lymphocytes. Phenomenon of synergism of low-dose γ-irradiation and mutagen S-Nitrosoglutathione as a ~3-fold increased frequency of chromatid-type aberrations (chemical markers) was detected compared to the sole radiation effect.
authors.forename: E A
authors.initials: EA
authors.lastname: Dyomina
authors.forename: O P
authors.initials: OP
authors.lastname: Pylypchuk
mesh_terms: D002478:Cells, Cultured; D002842:Chromatids / Q000187:drug effects / Q000528:radiation effects; D002869:Chromosome Aberrations / Q000139:chemically induced / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects; D009153:Mutagens / Q000633:toxicity*; D026422:S-Nitrosoglutathione / Q000633:toxicity*; D014700:Verapamil / Q000633:toxicity*
chemical_list: D009153:Mutagens; D026422:S-Nitrosoglutathione; D014700:Verapamil
keywords: S-Nitrosoglutathione; co-mutagenesis; human somatic cells; radiation; radiation-induced chromosomal aberrations; verapamil