title: Differential gene expression in proximal and distal nerve segments of rats with sciatic nerve injury during Wallerian degeneration.
abstract: Wallerian degeneration is a subject of major interest in neuroscience. A large number of genes are differentially regulated during the distinct stages of Wallerian degeneration: transcription factor activation, immune response, myelin cell differentiation and dedifferentiation. Although gene expression responses in the distal segment of the sciatic nerve after peripheral nerve injury are known, differences in gene expression between the proximal and distal segments remain unclear. In the present study in rats, we used microarrays to analyze changes in gene expression, biological processes and signaling pathways in the proximal and distal segments of sciatic nerves undergoing Wallerian degeneration. More than 6,000 genes were differentially expressed and 20 types of expression tendencies were identified, mainly between proximal and distal segments at 7-14 days after injury. The differentially expressed genes were those involved in cell differentiation, cytokinesis, neuron differentiation, nerve development and axon regeneration. Furthermore, 11 biological processes were represented, related to responses to stimuli, cell apoptosis, inflammatory response, immune response, signal transduction, protein kinase activity, and cell proliferation. Using real-time quantitative PCR, western blot analysis and immunohistochemistry, microarray data were verified for four genes: aquaporin-4, interleukin 1 receptor-like 1, matrix metalloproteinase-12 and periaxin. Our study identifies differential gene expression in the proximal and distal segments of a nerve during Wallerian degeneration, analyzes dynamic biological changes of these genes, and provides a useful platform for the detailed study of nerve injury and repair during Wallerian degeneration.
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China ; China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Jiang
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China.
authors.forename: Huaiqin
authors.initials: H
authors.lastname: Li
authors.affiliation: China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.affiliation: China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Dexin
authors.initials: D
authors.lastname: Yin
authors.affiliation: Key Laboratory of People's Liberation Army, Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Shusen
authors.initials: S
authors.lastname: Cui
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China.
authors.forename: Dengbing
authors.initials: D
authors.lastname: Yao
keywords: NSFC grant; Wallerian degeneration; biological process; expression profiling; microarray; nerve regeneration; neural regeneration; peripheral nerve injury; rat; sciatic nerve injury