title: Successful use of rFVIIa for major breast surgery prophylaxis in congenital factor VII deficiency: a case report.
abstract: BACKGROUND
  Factor VII deficiency is a rare cause of haemorrhagic syndrome. The Authors describe a case of a 46 years old patient with congenital factor VII deficiency that successfully underwent breast surgery after treatment with Novoseven® before the procedure.
  
  
  METHODS
  The AA used the schedule reported below to value the levels of PT and aPTT in the patient. Blood Collection: Venous blood from patient and control was collected in glass tubes for routine serum preparation and into plastic tubes (0.129 M sodium citrate, Becton-Dickinson Vacutainer Systems) in a ratio of blood to anticoagulant of 9:1. Platelet Poor Plasma (PPP) was obtained by centrifugation at 4.000 x g for 15 minutes at room temperature. The plasma was recentrifuged for another 10 min at 12000 g to fully eliminate platelet concentration. A normal control plasma pool was prepared by mixing equal volumes of platelet-free plasma obtained from at least 50 normal volunteers. Prothrombin time (PT) was measured with Recombiplastin (IL, Milano Italy). Activated partial thromboplastin times (APTT) was measured with APTT-SP (IL, Milano Italy). They were performed on the coagulation analyzer ACL 1000 (IL, Milano Italy).
  
  
  RESULTS
  The results were interpreted from the ratio of the patient times to the normal control times (Table I).
  
  
  CONCLUSIONS
  The infusion of Novoseven solved the clotting problems enabling the surgical procedure, without risks for the patient.
authors.forename: Antonio
authors.initials: A
authors.lastname: Varricchio
authors.forename: Michela
authors.initials: M
authors.lastname: Schettino
authors.forename: Antonio
authors.initials: A
authors.lastname: Ciampa
authors.forename: Carlo
authors.initials: C
authors.lastname: Iannace
authors.forename: Domenico
authors.initials: D
authors.lastname: Lo Conte
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Di Libero
authors.forename: Bernadette
authors.initials: B
authors.lastname: Donnarumma
authors.forename: Raffaella
authors.initials: R
authors.lastname: Vigorito
authors.forename: Mariolina
authors.initials: M
authors.lastname: Lepore
mesh_terms: D001943:Breast Neoplasms / Q000150:complications / Q000601:surgery; D005168:Factor VII Deficiency / Q000150:complications*; D015942:Factor VIIa / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D008875:Middle Aged; D010144:Paget's Disease, Mammary / Q000150:complications / Q000601:surgery; D019106:Postoperative Hemorrhage / Q000209:etiology* / Q000517:prevention & control*; D011300:Preoperative Care; D011994:Recombinant Proteins / Q000627:therapeutic use
chemical_list: D011994:Recombinant Proteins; C103587:recombinant FVIIa; D015942:Factor VIIa