title: Iron-chelating and anti-lipid peroxidation properties of 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one (CM1) in long-term iron loading β-thalassemic mice.
abstract: OBJECTIVE
  To evaluate the iron-chelating properties and free-radical scavenging activities of 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one (CM1) treatment in chronic iron-loaded β-thalassemic (BKO) mice.
  
  
  METHODS
  The BKO mice were fed with a ferrocene-rich diet and were orally administered with CM1 [50 mg/(kg.day)] for 6 months. Blood levels of non-transferrin bound iron, labile plasma iron, ferritin (Ft) and malondialdehyde were determined.
  
  
  RESULTS
  The BKO mice were fed with an iron diet for 8 months which resulted in iron overload. Interestingly, the mice showed a decrease in the non-transferrin bound iron, labile plasma iron and malondialdehyde levels, but not the Ft levels after continuous CM1 treatment.
  
  
  CONCLUSIONS
  CM1 could be an effective oral iron chelator that can reduce iron overload and lipid peroxidation in chronic iron overload β-thalassemic mice.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Kanokwan
authors.initials: K
authors.lastname: Kulprachakarn
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Nittaya
authors.initials: N
authors.lastname: Chansiw
authors.affiliation: College of Medicine and Public Health, Ubon Ratchathani University, Ubon Ratchathani, Thailand.
authors.forename: Kanjana
authors.initials: K
authors.lastname: Pangjit
authors.affiliation: Institute of Research and Development, Government Pharmaceuticals Organization, Ministry of Public Health, Thailand.
authors.forename: Chada
authors.initials: C
authors.lastname: Phisalaphong
authors.affiliation: Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University Salaya Campus, Nakornprathom, Thailand.
authors.forename: Suthat
authors.initials: S
authors.lastname: Fucharoen
authors.affiliation: Institute of Pharmaceutical Science, King's College London, Franklin-Wilkins Building, London, United Kingdom.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Hider
authors.affiliation: Medical Science Research Equipment Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Sineenart
authors.initials: S
authors.lastname: Santitherakul
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Somdet
authors.initials: S
authors.lastname: Srichairatanakool
keywords: Iron chelator; Iron overload; Iron-chelating; Lipid peroxidation; Non-transferrin bound iron; β-thalassemia