title: Protective effect of Jiaweibugan decoction against diabetic peripheral neuropathy.
abstract: Oxygen free radical damage is regarded as a direct or indirect common pathway associated with diabetic neuropathy and is the main cause of complications in peripheral neuropathies. We speculate that Jiaweibugan decoction has a significant effect in treating diabetic peripheral neuropathy through an anti-oxidative stress pathway. In this study, a diabetic rat model was established by intraperitoneal injection of streptozotocin. Rats were treated with Jiaweibugan decoction via intragastric administration. The levels of malondialdehyde and glutathione, which are indirect indexes of oxidative stress, in serum were determined using a colorimetric method. The expression levels of nuclear factor kappa B p65 mRNA and p38 mitogen-activated protein kinase, which are oxidative stress associated factors, in the dorsal root ganglion of spinal S4-6 segments were evaluated by reverse-transcriptase polymerase chain reaction and immunohistochemistry. Results showed that, Jiaweibugan decoction significantly ameliorated motor nerve conduction velocity in diabetic rats, effectively decreased malondialdehyde levels in serum and the expression of nuclear factor kappa B p65 mRNA and p38 mitogen-activated protein kinase mRNA in the dorsal root ganglion, and increased glutathione levels in serum. Therefore, our experimental findings indicate that Jiaweibugan decoction plays an anti-oxidative stress role in the diabetic peripheral neuropathy process, which has a protective effect on peripheral nerve injury.
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China.
authors.forename: Zeqi
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Internal Medicine, Chinese Medical Hospital in Bao'an District of Shenzhen City, Shenzhen 518133, Guangdong Province, China.
authors.forename: Renqun
authors.initials: R
authors.lastname: Ye
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China.
authors.forename: Yulei
authors.initials: Y
authors.lastname: He
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China.
authors.forename: Xinjian
authors.initials: X
authors.lastname: Qiu
keywords: Jiaweibugan decoction; diabetic peripheral neuropathy; glutathione; grants-supported paper; malondialdehyde; neural regeneration; neuroregeneration; nuclear factor kappa B p65; oxidative stress; oxygen free radical damage; p38 mitogen-activated protein kinase; peripheral nerve injury; streptozotocin; taurine; traditional Chinese medicine