title: Protein microarray for complex apoptosis  monitoring of dysplastic oral keratinocytes in  experimental photodynamic therapy.
abstract: BACKGROUND
  Photodynamic therapy is an alternative treatment of muco-cutaneous tumors that uses a light source able to photoactivate a chemical compound that acts as a photosensitizer. The phthalocyanines append to a wide chemical class that encompasses a large range of compounds; out of them aluminium-substituted disulphonated phthalocyanine possesses a good photosensitizing potential.
  
  
  RESULTS
  The destructive effects of PDT with aluminium-substituted disulphonated phthalocyanine are achieved by induction of apoptosis in tumoral cells as assessed by flow cytometry analysis. Using protein microarray we evaluate the possible molecular pathways by which photodynamic therapy activates apoptosis in dysplastic oral keratinocytes cells, leading to the tumoral cells destruction. Among assessed analytes, Bcl-2, P70S6K kinase, Raf-1 and Bad proteins represent the apoptosis related biomolecules that showed expression variations with the greatest amplitude.
  
  
  CONCLUSIONS
  Up to date, the intimate molecular apoptotic mechanisms activated by photodynamic therapy with this type of phthalocyanine in dysplastic human oral keratinocytes are not completely elucidated. With protein microarray as high-throughput proteomic approach a better understanding of the manner in which photodynamic therapy leads to tumoral cell destruction can be obtained, by depicting apoptotic molecules that can be potentially triggered in future anti-tumoral therapies.
authors.forename: Clara
authors.initials: C
authors.lastname: Matei
authors.forename: Mircea
authors.initials: M
authors.lastname: Tampa
authors.forename: Constantin
authors.initials: C
authors.lastname: Caruntu
authors.forename: Rodica-Mariana
authors.initials: RM
authors.lastname: Ion
authors.forename: Simona-Roxana
authors.initials: SR
authors.lastname: Georgescu
authors.forename: Georgiana Roxana
authors.initials: GR
authors.lastname: Dumitrascu
authors.forename: Carolina
authors.initials: C
authors.lastname: Constantin
authors.forename: Monica
authors.initials: M
authors.lastname: Neagu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D006801:Humans; D007211:Indoles / Q000627:therapeutic use; D015603:Keratinocytes / Q000187:drug effects* / Q000473:pathology; D009062:Mouth Neoplasms / Q000188:drug therapy* / Q000473:pathology; D009942:Organometallic Compounds / Q000627:therapeutic use; D010778:Photochemotherapy; D011230:Precancerous Conditions / Q000188:drug therapy* / Q000473:pathology; D040081:Protein Array Analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D019908:Proto-Oncogene Proteins c-raf / Q000032:analysis; D011838:Radiation-Sensitizing Agents / Q000627:therapeutic use; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000032:analysis; D051025:bcl-Associated Death Protein / Q000032:analysis
chemical_list: D007211:Indoles; D009942:Organometallic Compounds; D019253:Proto-Oncogene Proteins c-bcl-2; D011838:Radiation-Sensitizing Agents; D051025:bcl-Associated Death Protein; C060570:aluminum phthalocyanine; D019908:Proto-Oncogene Proteins c-raf; D038762:Ribosomal Protein S6 Kinases, 70-kDa