title: Nanoparticles carrying neurotrophin-3-modified Schwann cells promote repair of sciatic nerve defects.
abstract: Schwann cells and neurotrophin-3 play an important role in neural regeneration, but the secretion of neurotrophin-3 from Schwann cells is limited, and exogenous neurotrophin-3 is inactived easily in vivo. In this study, we have transfected neurotrophin-3 into Schwann cells cultured in vitro using nanoparticle liposomes. Results showed that neurotrophin-3 was successfully transfected into Schwann cells, where it was expressed effectively and steadily. A composite of Schwann cells transfected with neurotrophin-3 and poly(lactic-co-glycolic acid) biodegradable conduits was transplanted into rats to repair 10-mm sciatic nerve defects. Transplantation of the composite scaffold could restore the myoelectricity and wave amplitude of the sciatic nerve by electrophysiological examination, promote nerve axonal and myelin regeneration, and delay apoptosis of spinal motor neurons. Experimental findings indicate that neurotrophin-3 transfected Schwann cells combined with bridge grafting can promote neural regeneration and functional recovery after nerve injury.
authors.affiliation: Functional Laboratory, School of Basic Medical Sciences, Xinxiang Medical College, Xinxiang 453003, Henan Province, China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Zong
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, Henan Province, China.
authors.forename: Hongxing
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, Henan Province, China.
authors.forename: Yilei
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, Henan Province, China.
authors.forename: Jingling
authors.initials: J
authors.lastname: Jia
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, Henan Province, China.
authors.forename: Libin
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, Henan Province, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.affiliation: Functional Laboratory, School of Basic Medical Sciences, Xinxiang Medical College, Xinxiang 453003, Henan Province, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, Henan Province, China.
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Dong
keywords: Schwann cells; gene transfection; grants-supported paper; nanoparticle liposome; neural regeneration; neuroelectrophysiology; neuroregeneration; neurotrophin-3; peripheral nerve injury; sciatic nerve