title: Study of the efficacy of a Wheaton coated bottle with permethrin and deltamethrin in laboratory conditions and a WHO impregnated paper with bendiocarb in field conditions.
abstract: OBJECTIVE
  To determine the efficacy of WHO impregnated paper and CDC coated bottle based on number of storage days and number of times of consecutive use, in the assessment of insecticide vector susceptibility tests in laboratory and field conditions.
  
  
  METHODS
  Larvae and pupae of Anopheles gambiae s.l. mosquitoes were collected from the breeding sites in Seme-Kpodji and Cotonou districts in Southern Benin in April 2013 during the first rainy season. Anopheles gambiae s.l. mosquitoes were also collected from the breeding sites in Parakou district in Northern Benin in May 2013 at the beginning of the rainy season. Susceptibility tests were done using impregnated paper with bendiocarb (0.1%) following WHO protocol and stock solutions of permethrin (21.5 µg per bottle) and deltamethrin (12.5 µg per bottle) following CDC protocol on unfed female mosquitoes aged 2-5 days old. These bioassays were repeated a certain number of times. The temperature and relative humidity were monitored and recorded during the susceptibility tests.
  
  
  RESULTS
  This study showed that a WHO impregnated paper with bendiocarb could be used four times during four consecutive days in field conditions. Regarding a Wheaton coated bottle with permethrin or deltamethrin, they could be used at least three times during four consecutive days in laboratory conditions.
  
  
  CONCLUSIONS
  The day storage and the number of times that a WHO impregnated paper and a CDC coated bottle maintained their efficacy are useful in the assessment of insecticide vectors susceptibility tests.
authors.affiliation: Centre de Recherche Entomologique de Cotonou (CREC), 06 BP 2604, Cotonou, Bénin ; Faculté des Sciences et Techniques, Université d'Abomey Calavi, Calavi, Bénin.
authors.forename: Nazaire
authors.initials: N
authors.lastname: Aïzoun
authors.affiliation: Centre de Recherche Entomologique de Cotonou (CREC), 06 BP 2604, Cotonou, Bénin ; University of Massachusetts Amherst, Amherst, Massachusetts, USA.
authors.forename: Roseric
authors.initials: R
authors.lastname: Azondekon
authors.affiliation: Centre de Recherche Entomologique de Cotonou (CREC), 06 BP 2604, Cotonou, Bénin ; Faculté des Sciences et Techniques, Université d'Abomey Calavi, Calavi, Bénin.
authors.forename: Rock
authors.initials: R
authors.lastname: Aïkpon
authors.affiliation: Centre de Recherche Entomologique de Cotonou (CREC), 06 BP 2604, Cotonou, Bénin ; Faculté des Sciences et Techniques, Université d'Abomey Calavi, Calavi, Bénin.
authors.forename: Virgile
authors.initials: V
authors.lastname: Gnanguenon
authors.affiliation: Centre de Recherche Entomologique de Cotonou (CREC), 06 BP 2604, Cotonou, Bénin ; Faculté des Sciences et Techniques, Université d'Abomey Calavi, Calavi, Bénin.
authors.forename: Razaki
authors.initials: R
authors.lastname: Osse
authors.affiliation: London School of Hygiene and Tropical Medecine, Keppel Street WC1E 7HT, United Kingdom.
authors.forename: Alex
authors.initials: A
authors.lastname: Asidi
authors.affiliation: Centre de Recherche Entomologique de Cotonou (CREC), 06 BP 2604, Cotonou, Bénin ; Faculté des Sciences et Techniques, Université d'Abomey Calavi, Calavi, Bénin.
authors.forename: Martin
authors.initials: M
authors.lastname: Akogbéto
keywords: Coated bottle; Efficacy; Field conditions; Impregnated paper; Insecticide; Laboratory conditions