title: Site-specific, reversible and fluorescent immobilization of proteins on CrAsH-modified surfaces for microarray analytics.
abstract: A novel technique for protein immobilization onto CrAsH-modified surfaces is presented. This approach enables an efficient, reversible and fluorogenic immobilization of proteins. Moreover, expressed proteins can also be directly immobilized from cellular lysates without prior purification. The immobilized proteins are suitable for protein-protein interaction studies and the fluorescence enhancement upon immobilization can be employed for the direct detection of the immobilized protein without the need for secondary detection methods.
authors.affiliation: Max-Planck-Institut für Molekulare Physiologie, Abteilung Chemische Biologie, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany.
authors.forename: Janine
authors.initials: J
authors.lastname: Schulte-Zweckel
authors.forename: Federica
authors.initials: F
authors.lastname: Rosi
authors.forename: Domalapally
authors.initials: D
authors.lastname: Sreenu
authors.forename: Hendrik
authors.initials: H
authors.lastname: Schröder
authors.forename: Christof M
authors.initials: CM
authors.lastname: Niemeyer
authors.forename: Gemma
authors.initials: G
authors.lastname: Triola
mesh_terms: D020816:Amino Acid Motifs; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D008856:Microscopy, Fluorescence; D040081:Protein Array Analysis; D054730:Protein Interaction Domains and Motifs; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D013388:Succinimides / Q000737:chemistry; D013499:Surface Properties
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D055767:Immobilized Proteins; D011506:Proteins; D013388:Succinimides; C024098:6-carboxyfluorescein; C001426:N-hydroxysuccinimide