title: The calcification potential of human MSCs can be enhanced by interleukin-1β in osteogenic medium.
abstract: Inflammation is one of the key regulators of the repair process in bone tissues. Current data about the effect of interleukin-1β (IL-1β) on MSCs and osteoblasts are conflicting. We investigated the long-term effect of IL-1β on direct osteogenic differentiation of hMSCs in vitro. IL-1β-stimulated cells showed enhanced proliferation and entered maturation prior to non-stimulated ones, as monitored by ALP activity. The process of calcification was accelerated during long-term stimulation of hMSCs with IL-1β. Since donor variability is a well-known issue, we suggest a new method to illustrate global changes of a random chosen donor population through collative analysis. We further demonstrate an absorbance assay to evaluate the degree of calcification during in vitro culture of monolayer expanded hMSCs. Our findings support the importance of IL-1β in osteogenic differentiation of hMSCs in an in vitro monolayer culture model. A new online absorbance assay is a useful method to evaluate the osteogenic differentiation of hMSCs at early stages. These findings will be helpful in optimizing predifferentiation of hMSCs in vitro for bone tissue engineering. Copyright © 2014 John Wiley & Sons, Ltd.
authors.affiliation: AO Research Institute Davos, Davos Platz, Switzerland.
authors.forename: Claudia
authors.initials: C
authors.lastname: Loebel
authors.affiliation: AO Research Institute Davos, Davos Platz, Switzerland.
authors.forename: Ewa M
authors.initials: EM
authors.lastname: Czekanska
authors.affiliation: Department of Orthopaedic and Trauma Surgery, University Medical Centre, Albert-Ludwigs University, Freiburg, Germany.
authors.forename: Judith
authors.initials: J
authors.lastname: Staudacher
authors.affiliation: Department of Orthopaedic and Trauma Surgery, University Medical Centre, Albert-Ludwigs University, Freiburg, Germany.
authors.forename: Gian
authors.initials: G
authors.lastname: Salzmann
authors.affiliation: AO Research Institute Davos, Davos Platz, Switzerland.
authors.forename: R Geoff
authors.initials: RG
authors.lastname: Richards
authors.affiliation: AO Research Institute Davos, Davos Platz, Switzerland.
authors.forename: Mauro
authors.initials: M
authors.lastname: Alini
authors.affiliation: AO Research Institute Davos, Davos Platz, Switzerland.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Stoddart
mesh_terms: D000328:Adult; D000368:Aged; D001842:Bone and Bones / Q000166:cytology; D002113:Calcification, Physiologic; D002114:Calcinosis; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D005260:Female; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis / Q000187:drug effects*; D023822:Tissue Engineering / Q000379:methods; D055815:Young Adult
chemical_list: C508585:IL1B protein, human; D053583:Interleukin-1beta; D004247:DNA
keywords: Runx2; interleukin-1β; mesenchymal stromal cell; osteogenesis