title: Microsatellite primers in the foundation tree species Pinus edulis and P. monophylla (Pinaceae).
abstract: OBJECTIVE
  Microsatellite primers were developed in the foundational tree species Pinus edulis to investigate population differentiation of P. edulis and hybridization among closely related species. •
  
  
  RESULTS
  Using a hybridization protocol, primer sets for 11 microsatellite loci were developed using megagametophyte tissue from P. edulis and scored for polymorphism in three populations of P. edulis and a single P. monophylla population. The primers amplified simple and compound di-, tri-, and pentanucleotide repeats with two to 18 alleles per locus. •
  
  
  CONCLUSIONS
  These results demonstrate the utility of the described primers for studies of population differentiation within and among P. edulis populations as well as across putative hybrid zones where P. edulis may coexist with sister species.
authors.affiliation: Department of Biological Sciences, Northern Arizona University, 617 S. Beaver Street, Flagstaff, Arizona 86011 USA.
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Krohn
authors.affiliation: Department of Biological Sciences, Northern Arizona University, 617 S. Beaver Street, Flagstaff, Arizona 86011 USA.
authors.forename: Lluvia
authors.initials: L
authors.lastname: Flores-Rentería
authors.affiliation: Department of Biological Sciences, Northern Arizona University, 617 S. Beaver Street, Flagstaff, Arizona 86011 USA.
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Gehring
keywords: Pinaceae; Pinus edulis; Pinus monophylla; microsatellite; population genetics