title: Expression of mediators of purinergic signaling in human liver cell lines.
abstract: Purinergic signaling regulates a diverse and biologically relevant group of processes in the liver. However, progress of research into functions regulated by purinergic signals in the liver has been hampered by the complexity of systems probed. Specifically, there are multiple liver cell subpopulations relevant to hepatic functions, and many of these have been effectively modeled in human cell lines. Furthermore, there are more than 20 genes relevant to purinergic signaling, each of which has distinct functions. Hence, we felt the need to categorize genes relevant to purinergic signaling in the best characterized human cell line models of liver cell subpopulations. Therefore, we investigated the expression of adenosine receptor, P2X receptor, P2Y receptor, and ecto-nucleotidase genes via RT-PCR in the following cell lines: LX-2, hTERT, FH11, HepG2, Huh7, H69, and MzChA-1. We believe that our findings will provide an excellent resource to investigators seeking to define functions of purinergic signals in liver physiology and liver disease pathogenesis.
authors.affiliation: Division of Gastroenterology and Hepatology, University of Arkansas for Medical Sciences, Little Rock, AR, 72205, USA.
authors.forename: Jessica R
authors.initials: JR
authors.lastname: Goree
authors.forename: Elise G
authors.initials: EG
authors.lastname: Lavoie
authors.forename: Michel
authors.initials: M
authors.lastname: Fausther
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Dranoff
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D002460:Cell Line; D055166:Hepatic Stellate Cells / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000166:cytology / Q000378:metabolism; D011687:Purines / Q000378:metabolism*; D018047:Receptors, Purinergic P1 / Q000378:metabolism; D058469:Receptors, Purinergic P2X / Q000378:metabolism; D058470:Receptors, Purinergic P2Y / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D011687:Purines; D018047:Receptors, Purinergic P1; D058469:Receptors, Purinergic P2X; D058470:Receptors, Purinergic P2Y; D000251:Adenosine Triphosphatases; C027201:ectoATPase