title: Novonestmycins A and B, two new 32-membered bioactive macrolides from Streptomyces phytohabitans HBERC-20821.
abstract: Two new 32-membered macrolide compounds, named Novonestmycins A (1) and B (2), were isolated from the soil strain Streptomyces phytohabitans HBERC-20821. Their structures were elucidated by using spectroscopic methods, including 1D, 2D-NMR and MS spectrometry. The two compounds showed strong activities against the phytophathogenic fungi Corynespora cassiicola, Rhizoctonia solani and Septoria nodorum, with MIC values of 0.78, 0.39 and 0.78 μg ml(-1), respectively. In addition, the two compounds exhibited potent inhibitory activities against four different human tumor cell lines as well as one 5-FU-resistant human hepatocellular carcinoma cell line, with IC50 of 0.15-0.48 μg ml(-1) and 0.24-1.34 μg ml(-1), respectively.
authors.affiliation: College of Life Sciences, Wuhan University, Wuhan, PR China.
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Wan
authors.affiliation: National Engineering Research Center for Bio-pesticide, Hubei Academy of Agricultural Sciences, Wuhan, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Fang
authors.affiliation: National Engineering Research Center for Bio-pesticide, Hubei Academy of Agricultural Sciences, Wuhan, PR China.
authors.forename: Liqiao
authors.initials: L
authors.lastname: Shi
authors.affiliation: National Engineering Research Center for Bio-pesticide, Hubei Academy of Agricultural Sciences, Wuhan, PR China.
authors.forename: Kaimei
authors.initials: K
authors.lastname: Wang
authors.affiliation: National Engineering Research Center for Bio-pesticide, Hubei Academy of Agricultural Sciences, Wuhan, PR China.
authors.forename: Yani
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Engineering Research Center for Bio-pesticide, Hubei Academy of Agricultural Sciences, Wuhan, PR China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: National Engineering Research Center for Bio-pesticide, Hubei Academy of Agricultural Sciences, Wuhan, PR China.
authors.forename: Zhaoyuan
authors.initials: Z
authors.lastname: Wu
authors.affiliation: 1] College of Life Sciences, Wuhan University, Wuhan, PR China [2] National Engineering Research Center for Bio-pesticide, Hubei Academy of Agricultural Sciences, Wuhan, PR China.
authors.forename: Ziwen
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Syngenta Jealott's Hill International Research Centre, Bracknell, Berkshire, UK.
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Gu
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D006801:Humans; D018942:Macrolides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D012988:Soil Microbiology; D013302:Streptomyces / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D018942:Macrolides; C000598195:novonestmycin A; C000598196:novonestmycin B