title: Is longer sevoflurane preconditioning neuroprotective in permanent focal cerebral ischemia?
abstract: Sevoflurane preconditioning has neuroprotective effects in the cerebral ischemia/reperfusion model. However, its influence on permanent cerebral ischemia remains unclear. In the present study, the rats were exposed to sevoflurane for 15, 30, 60, and 120 minutes, followed by induction of permanent cerebral ischemia. Results demonstrated that 30- and 60-minute sevoflurane preconditioning significantly reduced the infarct volume at 24 hours after cerebral ischemia, and 60-minute lurane preconditioning additionally reduced the number of TUNEL- and caspase-3-positive cells in the ischemic penumbra. However, 120-minute sevoflurane preconditioning did not show evident neuroprotective effects. Moreover, 60-minute sevoflurane preconditioning significantly attenuated neurological deficits and infarct volume in rats at 4 days after cerebral ischemia. These findings indicated that 60-minute sevoflurane preconditioning can induce the best neuroprotective effects in rats with permanent cerebral ischemia through the inhibition of apoptosis.
authors.affiliation: West China School of Pharmacy, Sichuan University, Chengdu 610041, Sichuan Province, China ; Neuroscience Research Center, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Caiwei
authors.initials: C
authors.lastname: Qiu
authors.affiliation: Department of Anesthesiology, Panzhihua Central Hospital, Panzhihua 617000, Sichuan Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Sheng
authors.affiliation: Department of Neurology, the 452 People's Liberation Army Hospital, Chengdu 610041, Sichuan Province, China.
authors.forename: Shurong
authors.initials: S
authors.lastname: Wang
authors.affiliation: Neuroscience Research Center, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
keywords: anesthesia; apoptosis; brain injury; caspase-3; cerebral ischemia; neural regeneration; neuroregeneration; preconditioning; sevoflurane