title: Sleep deprivation induces differential morphological changes in the hippocampus and prefrontal cortex in young and old rats.
abstract: Sleep is a fundamental state necessary for maintenance of physical and neurological homeostasis throughout life. Several studies regarding the functions of sleep have been focused on effects of sleep deprivation on synaptic plasticity at a molecular and electrophysiological level, and only a few studies have studied sleep function from a structural perspective. Moreover, during normal aging, sleep architecture displays some changes that could affect normal development in the elderly. In this study, using a Golgi-Cox staining followed by Sholl analysis, we evaluate the effects of 24 h of total sleep deprivation on neuronal morphology of pyramidal neurons from Layer III of the prefrontal cortex (PFC) and the dorsal hippocampal CA1 region from male Wistar rats at two different ages (3 and 22 months). We found no differences in total dendritic length and branching length in both analyzed regions after sleep deprivation. Spine density was reduced in the CA1 of young-adults, and interestingly, sleep deprivation increased spine density in PFC of aged animals. Taken together, our results show that 24 h of total sleep deprivation have different effects on synaptic plasticity and could play a beneficial role in cognition during aging.
authors.affiliation: Department of Biomedicine, Health Sciences Institute, Veracruzana University, Luis Castelazo-Ayala s/n, Industrial-Animas, Xalapa, Veracruz, 91190, México.
authors.forename: Eva
authors.initials: E
authors.lastname: Acosta-Peña
authors.forename: Israel
authors.initials: I
authors.lastname: Camacho-Abrego
authors.forename: Montserrat
authors.initials: M
authors.lastname: Melgarejo-Gutiérrez
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Flores
authors.forename: René
authors.initials: R
authors.lastname: Drucker-Colín
authors.forename: Fabio
authors.initials: F
authors.lastname: García-García
mesh_terms: D000375:Aging / Q000473:pathology* / Q000502:physiology; D000818:Animals; D056547:CA1 Region, Hippocampal / Q000473:pathology* / Q000503:physiopathology; D048429:Cell Size; D003712:Dendrites / Q000473:pathology / Q000502:physiology; D004567:Electrodes, Implanted; D004569:Electroencephalography; D008297:Male; D017397:Prefrontal Cortex / Q000473:pathology* / Q000503:physiopathology; D017966:Pyramidal Cells / Q000473:pathology* / Q000502:physiology; D011897:Random Allocation; D017208:Rats, Wistar; D012890:Sleep / Q000502:physiology; D012892:Sleep Deprivation / Q000473:pathology* / Q000503:physiopathology; D014851:Wakefulness / Q000502:physiology
keywords: Golgi-Cox staining; aging; dendritic morphology; synaptic plasticity