title: Chloride channel blocker 4,4-diisothiocyanatostilbene-2,2'-disulfonic acid inhibits nitric oxide-induced apoptosis in cultured rat hippocampal neurons.
abstract: Apoptosis in cultured rat hippocampal neurons was induced using the nitric oxide donor 3-morpholinosydnonimine, and cells were treated with the chloride channel blocker, 4,4-diisothiocyanatostilbene-2,2'-disulfonic acid. Results showed that the survival rate of neurons was significantly increased after treatment with 4,4-diisothiocyanatostilbene-2,2'-disulfonic acid, and the rate of apoptosis decreased. In addition, the expression of the apoptosis-related proteins poly(adenosine diphosphate-ribose)polymerase-1 and apoptosis-inducing factor were significantly reduced. Our experimental findings indicate that the chloride channel blocker 4,4-diisothiocyanatostilbene-2,2'-disulfonic acid can antagonize apoptotic cell death of hippocampal neurons by inhibiting the expression of the apoptosis-related proteins poly(adenosine diphosphate-ribose)polymerase-1 and apoptosis-inducing factor.
authors.affiliation: Department of Physiology, Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, Guangdong Province, China ; Department of Pathology, Dongguan Campus of Guangdong Medical College, Dongguan 523808, Guangdong Province, China.
authors.forename: Jinbao
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of Physiology, Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, Guangdong Province, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Physiology, Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, Guangdong Province, China.
authors.forename: Shuling
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Physiology, Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, Guangdong Province, China.
authors.forename: Yuanyin
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Physiology, Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, Guangdong Province, China.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Department of Physiology, Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, Guangdong Province, China.
authors.forename: Hongling
authors.initials: H
authors.lastname: Fan
authors.affiliation: Department of Physiology, Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, Guangdong Province, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Physiology, Zhuhai Campus of Zunyi Medical College, Zhuhai 519041, Guangdong Province, China.
authors.forename: Quanzhong
authors.initials: Q
authors.lastname: Chang
keywords: 3-morpholinosydnonimine; hippocampus; apoptosis inducing factor; brain injury; chloride channel; grants-supported paper; neural regeneration; neuronal apoptosis; neuroregeneration; photographs-containing paper; poly(adenosine diphosphate-ribose)polymerase-1