title: Exploring the catalytic promiscuity of a new glycosyltransferase from Carthamus tinctorius.
abstract: The catalytic promiscuity of a new glycosyltransferase (UGT73AE1) from Carthamus tinctorius was explored. UGT73AE1 showed the capability to glucosylate a total of 19 structurally diverse types of acceptors and to generate O-, S-, and N-glycosides, making it the first reported trifunctional plant glycosyltransferase. The catalytic reversibility and regioselectivity were observed and modeled in a one-pot reaction transferring a glucose moiety from icariin to emodin. These findings demonstrate the potential versatility of UGT73AE1 in the glycosylation of bioactive natural products.
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College , 1 Xian Nong Tan Street, Beijing 100050, P.R. China.
authors.forename: Kebo
authors.initials: K
authors.lastname: Xie
authors.forename: Ridao
authors.initials: R
authors.lastname: Chen
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.forename: Ruishan
authors.initials: R
authors.lastname: Wang
authors.forename: Dawei
authors.initials: D
authors.lastname: Chen
authors.forename: Xiaoxiang
authors.initials: X
authors.lastname: Dou
authors.forename: Jungui
authors.initials: J
authors.lastname: Dai
mesh_terms: D031187:Carthamus tinctorius / Q000201:enzymology*; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D004642:Emodin / Q000378:metabolism; D005419:Flavonoids / Q000378:metabolism; D006027:Glycosides / Q000737:chemistry / Q000378:metabolism; D006031:Glycosylation; D016695:Glycosyltransferases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure
chemical_list: D005419:Flavonoids; D006027:Glycosides; D016695:Glycosyltransferases; D004642:Emodin; C056599:icariin