title: From genes to protein mechanics on a chip.
abstract: Single-molecule force spectroscopy enables mechanical testing of individual proteins, but low experimental throughput limits the ability to screen constructs in parallel. We describe a microfluidic platform for on-chip expression, covalent surface attachment and measurement of single-molecule protein mechanical properties. A dockerin tag on each protein molecule allowed us to perform thousands of pulling cycles using a single cohesin-modified cantilever. The ability to synthesize and mechanically probe protein libraries enables high-throughput mechanical phenotyping.
authors.affiliation: Lehrstuhl für Angewandte Physik, Ludwig-Maximilians-Universität, 80799 Munich, Germany.|Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität, 80799 Munich, Germany.
authors.forename: Marcus
authors.initials: M
authors.lastname: Otten
authors.affiliation: Lehrstuhl für Angewandte Physik, Ludwig-Maximilians-Universität, 80799 Munich, Germany.|Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität, 80799 Munich, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Ott
authors.affiliation: Lehrstuhl für Angewandte Physik, Ludwig-Maximilians-Universität, 80799 Munich, Germany.|Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität, 80799 Munich, Germany.
authors.forename: Markus A
authors.initials: MA
authors.lastname: Jobst
authors.affiliation: Lehrstuhl für Angewandte Physik, Ludwig-Maximilians-Universität, 80799 Munich, Germany.|Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität, 80799 Munich, Germany.
authors.forename: Lukas F
authors.initials: LF
authors.lastname: Milles
authors.affiliation: Lehrstuhl für Angewandte Physik, Ludwig-Maximilians-Universität, 80799 Munich, Germany.|Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität, 80799 Munich, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Verdorfer
authors.affiliation: Lehrstuhl für Angewandte Physik, Ludwig-Maximilians-Universität, 80799 Munich, Germany.|Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität, 80799 Munich, Germany.|Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians-Universität, 80799 Munich, Germany.
authors.forename: Diana A
authors.initials: DA
authors.lastname: Pippig
authors.affiliation: Lehrstuhl für Angewandte Physik, Ludwig-Maximilians-Universität, 80799 Munich, Germany.|Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität, 80799 Munich, Germany.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Nash
authors.affiliation: Lehrstuhl für Angewandte Physik, Ludwig-Maximilians-Universität, 80799 Munich, Germany.|Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität, 80799 Munich, Germany.
authors.forename: Hermann E
authors.initials: HE
authors.lastname: Gaub
mesh_terms: D048013:Acetivibrio thermocellus / Q000235:genetics; D057166:High-Throughput Screening Assays; D046210:Microfluidic Analytical Techniques; D018625:Microscopy, Atomic Force / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis; D019151:Peptide Library; D040081:Protein Array Analysis / Q000379:methods*
chemical_list: D019151:Peptide Library