title: Carboxyl-terminal-dependent recruitment of nonmuscle myosin II to megakaryocyte contractile ring during polyploidization.
abstract: Endomitosis is a unique megakaryocyte (MK) differentiation process that is the consequence of a late cytokinesis failure associated with a contractile ring defect. Evidence from in vitro studies has revealed the distinct roles of 2 nonmuscle myosin IIs (NMIIs) on MK endomitosis: only NMII-B (MYH10), but not NMII-A (MYH9), is localized in the MK contractile ring and implicated in mitosis/endomitosis transition. Here, we studied 2 transgenic mouse models in which nonmuscle myosin heavy chain (NMHC) II-A was genetically replaced either by II-B or by a chimeric NMHCII that combined the head domain of II-A with the rod and tail domains of II-B. This study provides in vivo evidence on the specific role of NMII-B on MK polyploidization. It demonstrates that the carboxyl-terminal domain of the heavy chains determines myosin II localization to the MK contractile ring and is responsible for the specific role of NMII-B in MK polyploidization.
authors.affiliation: INSERM UMR1009, Institut Gustave Roussy, Villejuif, France; Université Paris-Sud, Unité Mixte de Recherche 1009, Le Kremlin-Bicêtre, France; Institut Gustave Roussy, Unité Mixte de Recherche 1009, Villejuif, France; and.
authors.forename: Idinath
authors.initials: I
authors.lastname: Badirou
authors.affiliation: INSERM UMR1009, Institut Gustave Roussy, Villejuif, France; Université Paris-Sud, Unité Mixte de Recherche 1009, Le Kremlin-Bicêtre, France; Institut Gustave Roussy, Unité Mixte de Recherche 1009, Villejuif, France; and.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Pan
authors.affiliation: INSERM UMR1009, Institut Gustave Roussy, Villejuif, France; Université Paris-Sud, Unité Mixte de Recherche 1009, Le Kremlin-Bicêtre, France; Institut Gustave Roussy, Unité Mixte de Recherche 1009, Villejuif, France; and.
authors.forename: Céline
authors.initials: C
authors.lastname: Legrand
authors.affiliation: Laboratory of Molecular Cardiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD.
authors.forename: Aibing
authors.initials: A
authors.lastname: Wang
authors.affiliation: INSERM UMR1009, Institut Gustave Roussy, Villejuif, France; Université Paris-Sud, Unité Mixte de Recherche 1009, Le Kremlin-Bicêtre, France; Institut Gustave Roussy, Unité Mixte de Recherche 1009, Villejuif, France; and.
authors.forename: Larissa
authors.initials: L
authors.lastname: Lordier
authors.affiliation: INSERM UMR1009, Institut Gustave Roussy, Villejuif, France; Université Paris-Sud, Unité Mixte de Recherche 1009, Le Kremlin-Bicêtre, France; Institut Gustave Roussy, Unité Mixte de Recherche 1009, Villejuif, France; and.
authors.forename: Siham
authors.initials: S
authors.lastname: Boukour
authors.affiliation: INSERM UMR1009, Institut Gustave Roussy, Villejuif, France; Université Paris-Sud, Unité Mixte de Recherche 1009, Le Kremlin-Bicêtre, France; Institut Gustave Roussy, Unité Mixte de Recherche 1009, Villejuif, France; and.
authors.forename: Anita
authors.initials: A
authors.lastname: Roy
authors.affiliation: INSERM UMR1009, Institut Gustave Roussy, Villejuif, France; Université Paris-Sud, Unité Mixte de Recherche 1009, Le Kremlin-Bicêtre, France; Institut Gustave Roussy, Unité Mixte de Recherche 1009, Villejuif, France; and.
authors.forename: William
authors.initials: W
authors.lastname: Vainchenker
authors.affiliation: INSERM UMR1009, Institut Gustave Roussy, Villejuif, France; Université Paris-Sud, Unité Mixte de Recherche 1009, Le Kremlin-Bicêtre, France; Institut Gustave Roussy, Unité Mixte de Recherche 1009, Villejuif, France; and.
authors.forename: Yunhua
authors.initials: Y
authors.lastname: Chang
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D008533:Megakaryocytes / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008938:Mitosis; D018995:Myosin Heavy Chains / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D024507:Nonmuscle Myosin Type IIA / Q000737:chemistry / Q000235:genetics; D024501:Nonmuscle Myosin Type IIB / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D011123:Polyploidy; D017434:Protein Structure, Tertiary
chemical_list: D024507:Nonmuscle Myosin Type IIA; D024501:Nonmuscle Myosin Type IIB; C103815:nonmuscle myosin type IIB heavy chain; D018995:Myosin Heavy Chains