title: An improved method for rapid generation and screening of Bacillus thuringiensis phage-resistant mutants.
abstract: A simple method to isolate, screen and select phage-resistant mutants of Bacillus thuringiensis was developed. The traditional double-layer agar method was improved by a combination of the spotting assay using a lytic phage, to generate the bacterial-resistant mutants, with an inverted spotting assay (ISA), to rapidly screen the candidate-resistant mutants.
authors.affiliation: Laboratory of Food and Environmental Microbiology, Université catholique de Louvain, Croix du Sud, Louvain-la-Neuve, Belgium. Electronic address: annika.gillis@uclouvain.be.
authors.forename: Annika
authors.initials: A
authors.lastname: Gillis
authors.affiliation: Laboratory of Food and Environmental Microbiology, Université catholique de Louvain, Croix du Sud, Louvain-la-Neuve, Belgium.
authors.forename: Jacques
authors.initials: J
authors.lastname: Mahillon
mesh_terms: D017103:Bacillus Phages / Q000254:growth & development*; D001413:Bacillus thuringiensis / Q000821:virology*; D008828:Microbiological Techniques / Q000379:methods; D009154:Mutation
keywords: Bacillus thuringiensis; Phage; Phage-resistant mutants; Tectivirus