title: Leptin does not enhance cell-mediated immune responses following mycobacterial antigen stimulation.
abstract: BACKGROUND
  Tuberculosis (TB) is a infectious disease characterised by a profound immune-endocrine metabolic imbalance, including a diminution in leptin plasma levels. Leptin appears to be the link between nutritional status and the development of a protective immune response.
  
  
  OBJECTIVE
  To examine the effects of leptin on the proliferation and production of interferon-gamma (IFN-γ) by peripheral blood mononuclear cells (PBMC) in TB patients and healthy controls stimulated with mycobacterial antigens with or without leptin. As macrophages are key cells in mycobacterial containment, the effect of leptin on the production of interleukin (IL) 1β and IL-1Ra by the monocytic cell line THP-1 was also studied.
  
  
  RESULTS
  Leptin diminished the proliferative capacity of PBMC on mycobacterial stimulation, and had no effect on IFN-γ production in terms of measurements in culture supernatants or intracytoplasmic analysis using flow cytometry. Real-time polymerase chain reaction studies of PBMC from TB patients revealed a preserved expression of leptin receptor. Furthermore, IL-1β and IL-1Ra secretion by THP-1 cells was not modified by leptin treatment.
  
  
  CONCLUSIONS
  The study results do not support the utility of treatment with leptin to correct immune imbalances due to TB.
authors.affiliation: Institute of Immunology, School of Medical Sciences, National University of Rosario, Santa Fe, Argentina.
authors.forename: N
authors.initials: N
authors.lastname: Santucci
authors.affiliation: Institute of Immunology, School of Medical Sciences, National University of Rosario, Santa Fe, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Díaz
authors.affiliation: Institute of Immunology, School of Medical Sciences, National University of Rosario, Santa Fe, Argentina.
authors.forename: E
authors.initials: E
authors.lastname: Bianchi
authors.affiliation: Institute of Immunology, School of Medical Sciences, National University of Rosario, Santa Fe, Argentina.
authors.forename: S
authors.initials: S
authors.lastname: Spinelli
authors.affiliation: Institute of Immunology, School of Medical Sciences, National University of Rosario, Santa Fe, Argentina.
authors.forename: L
authors.initials: L
authors.lastname: D'Attilio
authors.affiliation: Institute of Immunology, School of Medical Sciences, National University of Rosario, Santa Fe, Argentina.
authors.forename: B
authors.initials: B
authors.lastname: Bongiovanni
authors.affiliation: Institute of Immunology, School of Medical Sciences, National University of Rosario, Santa Fe, Argentina.
authors.forename: G
authors.initials: G
authors.lastname: Dídoli
authors.affiliation: Respiratory Diseases, Hospital Escuela Eva Perón, Granadero Baigorria Santa Fe, Argentina.
authors.forename: N
authors.initials: N
authors.lastname: Brandan
authors.affiliation: Respiratory Diseases, Hospital Escuela Eva Perón, Granadero Baigorria Santa Fe, Argentina.
authors.forename: L
authors.initials: L
authors.lastname: Nannini
authors.affiliation: Institute of Immunology, School of Medical Sciences, National University of Rosario, Santa Fe, Argentina.
authors.forename: M L
authors.initials: ML
authors.lastname: Bay
authors.affiliation: Institute of Immunology, School of Medical Sciences, National University of Rosario, Santa Fe, Argentina.
authors.forename: O
authors.initials: O
authors.lastname: Bottasso
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology*; D016022:Case-Control Studies; D002460:Cell Line; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000276:immunology*; D053590:Interleukin 1 Receptor Antagonist Protein / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D020738:Leptin / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D009000:Monocytes / Q000187:drug effects / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology; D060888:Real-Time Polymerase Chain Reaction; D014376:Tuberculosis / Q000276:immunology*; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; D053590:Interleukin 1 Receptor Antagonist Protein; D053583:Interleukin-1beta; D020738:Leptin; D007371:Interferon-gamma