title: Discovery of EST-derived microsatellite primers in the legume Lens culinaris (Fabaceae).
abstract: OBJECTIVE
  We developed microsatellite markers in the legume Lens culinaris from publicly available databases to enrich the limited marker resource available for the crop. •
  
  
  RESULTS
  Eighty-two primer sets were identified using expressed sequence sets of L. culinaris available in the National Center for Biotechnology Information (NCBI) database and were characterized in six species of Lens. Among them, 20 simple sequence repeat (SSR) primers produced no amplification product, 43 produced monomorphic products, and 19 were polymorphic. The primers amplified mono-, di-, tri-, tetra-, penta-, and hexanucleotide repeats with one to four alleles. These SSR loci successfully amplified in five related wild species, with a total of 61 primer pairs in L. nigricans and L. odemensis (98.39%), 59 in L. tomentosus (95.1%), and 60 in L. ervoides and L. orientalis (96.7%), respectively. •
  
  
  CONCLUSIONS
  The microsatellite markers discovered in this study will be useful in genetic mapping, marker-assisted breeding, and characterization of germplasm.
authors.affiliation: Division of Genetics, Indian Agricultural Research Institute, New Delhi 110012, India.
authors.forename: Neelu
authors.initials: N
authors.lastname: Jain
authors.affiliation: Division of Genetics, Indian Agricultural Research Institute, New Delhi 110012, India.
authors.forename: H K
authors.initials: HK
authors.lastname: Dikshit
authors.affiliation: Division of Genetics, Indian Agricultural Research Institute, New Delhi 110012, India.
authors.forename: D
authors.initials: D
authors.lastname: Singh
authors.affiliation: Division of Genetics, Indian Agricultural Research Institute, New Delhi 110012, India.
authors.forename: Akanksha
authors.initials: A
authors.lastname: Singh
authors.affiliation: Division of Genetics, Indian Agricultural Research Institute, New Delhi 110012, India.
authors.forename: Harish
authors.initials: H
authors.lastname: Kumar
keywords: EST-SSRs; Fabaceae; Lens culinaris; microsatellites