title: Structure of glutathione S-transferase 1 from the major human hookworm parasite Necator americanus (Na-GST-1) in complex with glutathione.
abstract: Glutathione S-transferase 1 from Necator americanus (Na-GST-1) is a vaccine candidate for hookworm infection that has a high affinity for heme and metal porphyrins. As part of attempts to clarify the mechanism of heme detoxification by hookworm GSTs, co-crystallization and soaking studies of Na-GST-1 with the heme-like molecules protoporphyrin IX disodium salt, hematin and zinc protoporphyrin were undertaken. While these studies did not yield the structure of the complex of Na-GST-1 with any of these molecules, co-crystallization experiments resulted in the first structures of the complex of Na-GST-1 with the substrate glutathione. The structures of the complex of Na-GST-1 with glutathione were solved from pathological crystalline aggregates comprising more than one crystal form. These first structures of the complex of Na-GST-1 with the substrate glutathione were solved by molecular replacement from data collected with a sealed-tube home source using the previously reported apo structure as the search model.
authors.affiliation: National School of Tropical Medicine, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Oluwatoyin A
authors.initials: OA
authors.lastname: Asojo
authors.affiliation: National School of Tropical Medicine, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Ceccarelli
mesh_terms: D000818:Animals; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D005978:Glutathione / Q000737:chemistry*; D005982:Glutathione Transferase / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D017221:Necator americanus / Q000201:enzymology*
chemical_list: D005982:Glutathione Transferase; D005978:Glutathione
keywords: Necator americanus; glutathione S-transferase