title: Effects of recombinant IL-17F intranasal inoculation against Streptococcus pneumoniae infection in a murine model.
abstract: Interleukin-17F (IL-17F) is an important member of IL-17 cytokine family, which plays important roles in host defense against microbial infections. Streptococcus pneumoniae is a common pathogen associated with several invasive and noninvasive pneumococcal diseases, and mucosal immune response plays crucial roles in defenses against pneumococcal infection. Thus, intranasal inoculation may be an alternative approach against pneumococci. In this study, BALB/c mice were intranasally inoculated with recombinant IL-17F (rIL-17F) prior to S. pneumoniae (American Type Culture Collection 6303, serotype 3) infection. As compared with the control group, numbers of total leukocyte, neutrophil, and macrophage in lungs were significantly increased in mice inoculated with rIL-17F. The levels of macrophage inflammatory protein 1α (MIP-1α), MIP-2β, and interferon γ were significantly increased in bronchoalveolar lavage fluid and culture supernatant of splenocytes from mice inoculated with rIL-17F. rIL-17F inoculation also significantly elevated β-defensin-2 expression in lung tissues. Furthermore, compared with S. pneumoniae infection group, rIL-17F inoculation prior to infection significantly reduced S. pneumoniae colonization in lungs. These findings demonstrated that rIL-17F intranasal inoculation strengthened host defense against pneumococci, which may be developed to prevent pneumococcal infection.
authors.affiliation: Department of Pediatrics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai, 200233, People's Republic of China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Pediatrics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai, 200233, People's Republic of China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Pediatrics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai, 200233, People's Republic of China.
authors.forename: Liangxia
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Pediatrics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai, 200233, People's Republic of China.
authors.forename: Chunli
authors.initials: C
authors.lastname: Hao
authors.affiliation: Department of Pediatrics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai, 200233, People's Republic of China.
authors.forename: Wanting
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Pediatrics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai, 200233, People's Republic of China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D016207:Cytokines / Q000378:metabolism; D005260:Female; D020381:Interleukin-17 / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D011008:Pneumococcal Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology; D013296:Streptococcus pneumoniae / Q000276:immunology
chemical_list: D016207:Cytokines; D020381:Interleukin-17; D011994:Recombinant Proteins
keywords: Streptococcus pneumoniae; inflammation; interleukin-17F; β-defensin-2