title: Glucocorticoid receptor DNA binding factor 1 expression and osteosarcoma prognosis.
abstract: Glucocorticoid receptor DNA binding factor 1 (GRF-1) is an important Rho family GTPase-activating protein, and the dysregulation of GRF-1 expression maybe involved in tumor progression. However, the role of GRF-1 expression in the osteosarcoma prognosis has been well less elaborated. Here, we conducted a hospital-based case study, including 247 patients with pathologically confirmed osteosarcoma to evaluate the associations between GRF-1 expression and osteosarcoma prognosis. We found that high GRF-1 expression was correlated with poor outcome of osteosarcoma compared with low GRF-1 expression (the median recurrence-free survival times, 11 months vs 56 months; the median overall survival times, 18 months vs 53 months). Like tumor stage, the GRF-1 expression was an independent prognostic factor influencing the survival of osteosarcoma [hazard ratio values (95 % confidence interval) were 5.39 (3.54-8.20) for recurrence-free survival (RFS) and 6.58 (4.44-9.74) for overall survival (OS), respectively]. Furthermore, the high expression of GRF-1 was significantly associated with larger tumor size, tumor dedifferentiation, and increasing metastasis risk. Functionally, the knockdown of GRF-1 expression inhibited tumor cells proliferation and induced cell apoptosis. These results indicate for the first time that GRF-1 expression may modify osteosarcoma prognosis and may be a potential tumor therapeutic target.
authors.affiliation: Department of Orthopedic Trauma and Hand Surgery, Research Center for Regenerative Medicine, The First Affiliated Hospital of Guangxi Medical University, No.6 Shuang Yong Rd., Nanning, Guangxi, China.
authors.forename: Jinmin
authors.initials: J
authors.lastname: Zhao
authors.forename: Hua
authors.initials: H
authors.lastname: Xu
authors.forename: Maolin
authors.initials: M
authors.lastname: He
authors.forename: Yang
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D001859:Bone Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D016017:Odds Ratio; D012516:Osteosarcoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D011379:Prognosis; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D012307:Risk Factors; D047368:Tumor Burden; D055815:Young Adult
chemical_list: C074560:ARHGAP35 protein, human; D020662:Guanine Nucleotide Exchange Factors; D012097:Repressor Proteins