title: Lentivirus carrying the Atoh1 gene infects normal rat cochlea.
abstract: Lentivirus carrying the Atoh1 gene can infect Corti's organ and express a hair-like cell surface marker in the supporting cell area. However, expression of the gene carried by adenovirus is instantaneous, which undoubtedly limits its clinical application. Lentivirus acts as a carrier that can stably and continuously express genes. In this study, the cochlear structure and hearing level were not affected, and Atoh1 gene carried by lentivirus promoted the production of hair-like cells in the cochlear supporting cell area. This led to expression of the hair-like cell surface marker myosin 7a 30 days after lentivirus carrying Atoh1 was microinjected into the cochlear round window of rats.
authors.affiliation: Department of Otolaryngology, Guangming New District People's Hospital, Shenzhen 518106, Guangdong Province, China.
authors.forename: Song
authors.initials: S
authors.lastname: Pan
authors.affiliation: Department of Surgery, Hubei University of Science and Technology, Xianning 437100, Hubei Province, China.
authors.forename: Jingzhi
authors.initials: J
authors.lastname: Wan
authors.affiliation: Department of Otolaryngology, Beilun Branch of the First Affiliated Hospital, College of Medicine, Zhejiang University, Ningbo 315800, Zhejiang Province, China.
authors.forename: Shaosheng
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Otolaryngology, Guangming New District People's Hospital, Shenzhen 518106, Guangdong Province, China.
authors.forename: Song
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Xiong
authors.affiliation: Department of Otolaryngology, Guangming New District People's Hospital, Shenzhen 518106, Guangdong Province, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Otolaryngology, Guangming New District People's Hospital, Shenzhen 518106, Guangdong Province, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Xiong
authors.affiliation: Department of Otolaryngology, Guangming New District People's Hospital, Shenzhen 518106, Guangdong Province, China.
authors.forename: Kunfei
authors.initials: K
authors.lastname: Yu
authors.affiliation: Department of Otolaryngology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Fu
keywords: Atoh1; auditory threshold; cochlea; gene therapy; grants-supported paper; hair cells; lentivirus; myosin 7a; neural regeneration; neuroregeneration