title: Cloning, purification, crystallization and preliminary X-ray studies of a carbohydrate-binding module (CBM_E1) derived from sugarcane soil metagenome.
abstract: In recent years, owing to the growing global demand for energy, dependence on fossil fuels, limited natural resources and environmental pollution, biofuels have attracted great interest as a source of renewable energy. However, the production of biofuels from plant biomass is still considered to be an expensive technology. In this context, the study of carbohydrate-binding modules (CBMs), which are involved in guiding the catalytic domains of glycoside hydrolases for polysaccharide degradation, is attracting growing attention. Aiming at the identification of new CBMs, a sugarcane soil metagenomic library was analyzed and an uncharacterized CBM (CBM_E1) was identified. In this study, CBM_E1 was expressed, purified and crystallized. X-ray diffraction data were collected to 1.95 Å resolution. The crystals, which were obtained by the sitting-drop vapour-diffusion method, belonged to space group I23, with unit-cell parameters a = b = c = 88.07 Å.
authors.affiliation: Laboratório Nacional de Biociências (LNBio), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Campinas, São Paulo, Brazil.
authors.forename: Bruna Medeia
authors.initials: BM
authors.lastname: Campos
authors.affiliation: Laboratório Nacional de Ciências e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Campinas, São Paulo, Brazil.
authors.forename: Thabata Maria
authors.initials: TM
authors.lastname: Alvarez
authors.affiliation: Laboratório Nacional de Ciências e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Campinas, São Paulo, Brazil.
authors.forename: Marcelo Vizona
authors.initials: MV
authors.lastname: Liberato
authors.affiliation: Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, São Paulo, Brazil.
authors.forename: Igor
authors.initials: I
authors.lastname: Polikarpov
authors.affiliation: Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, Newcastle upon Tyne, England.
authors.forename: Harry J
authors.initials: HJ
authors.lastname: Gilbert
authors.affiliation: Laboratório Nacional de Biociências (LNBio), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Campinas, São Paulo, Brazil.
authors.forename: Ana Carolina de Mattos
authors.initials: AC
authors.lastname: Zeri
authors.affiliation: Laboratório Nacional de Ciências e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Campinas, São Paulo, Brazil.
authors.forename: Fabio Marcio
authors.initials: FM
authors.lastname: Squina
mesh_terms: D001483:Base Sequence; D002241:Carbohydrates / Q000737:chemistry*; D020134:Catalytic Domain; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D017931:DNA Primers; D056186:Metagenomics; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D031786:Saccharum; D012988:Soil Microbiology
chemical_list: D002241:Carbohydrates; D017931:DNA Primers; D010940:Plant Proteins
keywords: GH5; accessory domain; biofuels; carbohydrate-binding module; glycoside hydrolases; renewable energy