title: Collagen surfaces modified with photo-cleavable polyethylene glycol-lipid support versatile single-cell arrays of both non-adherent and adherent cells.
abstract: Cell patterning on photo-responsive materials are a promising tool for preparing unique single-cell arrays. However, most conventional single-cell arrays on such smart materials can be applied only to adherent cells and limit cellular functions such as extension and migration within the patterned adhesive surfaces. In this study, a versatile single cell array that works with both non-adherent and adherent cells was constructed using a photo-cleavable polyethylene glycol (PEG)-lipid/collagen surface. On this single-cell array, cells behaved similar to their native functions without limitation from the patterned surface. Furthermore, quantitative imaging analyses of cellular motility and morphological changes were performed in a high-throughput manner.
authors.affiliation: Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Yamahira
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kawahara
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nagamune
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D003094:Collagen / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D011092:Polyethylene Glycols / Q000737:chemistry*; D046888:Tissue Array Analysis / Q000379:methods*
chemical_list: D008055:Lipids; D011092:Polyethylene Glycols; D003094:Collagen
keywords: cell motility; high-throughput analyses; imaging cytometry; photo-responsible materials; single-cell arrays