title: Impaired arteriogenesis in syndecan-1(-/-) mice.
abstract: BACKGROUND
  Collateral artery development (arteriogenesis) is an important compensatory response to arterial occlusion caused by atherosclerosis. The heparan sulfate proteoglycan syndecan-1 (sdc1) has previously been shown to affect the response to arterial injury but has yet been studied in arteriogenesis. We tested the hypothesis that sdc1 knockout (sdc1(-/-)) mice would revascularize more poorly than wild type (wt) mice, and then used bone marrow transplantation experiments to determine whether sdc1's effect on arteriogenesis was due to its presence in the local tissue environment or in bone marrow derived cells.
  
  
  METHODS
  Hindlimb ischemia was induced by femoral artery ligation in wt and sdc1(-/-) female mice as well as in wt and sdc1(-/-) female mice transplanted with wt bone marrow or in wt mice transplanted with sdc1(-/-) bone marrow. Blood flow recovery was assessed by laser Doppler perfusion imaging. Arteriogenesis was assessed by measuring the diameter of the dominant collateral pathway after pressure perfusion fixation and intra-aortic contrast injection at 28 d. Immunohistochemistry was used to assess angiogenesis and peri-collateral macrophage infiltration at 7 d, postoperatively.
  
  
  RESULTS
  Sdc1(-/-) mice had impaired blood flow recovery in response to hindlimb ischemia. This impaired recovery was not secondary to a defect in capillary angiogenesis nor was it due to decreased peri-collateral macrophage infiltration. Wt bone marrow did not rescue the impaired recovery of sdc1(-/-) mice.
  
  
  CONCLUSIONS
  Sdc1 affects arteriogenesis in response to hindlimb ischemia and is required in the local tissue environment for normal arteriogenesis.
authors.affiliation: VA Puget Sound Health Care System, University of Washington, Seattle, Washington; Division of Vascular Surgery, Department of Surgery, University of Washington, Seattle, Washington. Electronic address: gtang@uw.edu.
authors.forename: Gale L
authors.initials: GL
authors.lastname: Tang
authors.affiliation: Division of Vascular Surgery, Department of Surgery, University of Washington, Seattle, Washington.
authors.forename: Kevin
authors.initials: K
authors.lastname: Weitz
mesh_terms: D000818:Animals; D001157:Arterial Occlusive Diseases / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D016026:Bone Marrow Transplantation; D002196:Capillaries / Q000502:physiology; D060833:Cellular Microenvironment / Q000502:physiology; D003097:Collateral Circulation / Q000235:genetics / Q000502:physiology*; D005260:Female; D005263:Femoral Artery / Q000000981:diagnostic imaging / Q000502:physiology; D006614:Hindlimb / Q000098:blood supply; D017078:Laser-Doppler Flowmetry; D008264:Macrophages / Q000502:physiology; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D053668:Syndecan-1 / Q000235:genetics* / Q000378:metabolism; D014463:Ultrasonography
chemical_list: C508242:Sdc1 protein, mouse; D053668:Syndecan-1
keywords: Angiogenesis; Arteriogenesis; Mouse hindlimb ischemia; Syndecan-1