title: Surface plasmon field-enhanced fluorescence spectroscopy apparatus with a convergent optical system for point-of-care testing.
abstract: Surface plasmon field-enhanced fluorescence spectroscopy (SPFS) is a promising methodology for point-of-care (POC) testing. The SPFS devices that have been reported are equipped with an angle rotating stage to adjust the surface plasmon resonance (SPR) angle. In a clinical setting, however, the SPR angle determination is a tedious and time-consuming process. In this study, we employed an SPFS instrument with a convergent optical system that allows the omission of this procedure. We demonstrated that this instrumentation allowed the sensitive determination of low concentrations of α-fetoprotein in serum and reduced the variation effect caused by the protein concentrations in samples. The SPFS with a convergent optical system is suitable for POC testing.
authors.affiliation: Institute for Frontier Medical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Toda
authors.affiliation: Institute for Frontier Medical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Arima
authors.affiliation: Graduate School of Engineering, University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Takiguchi
authors.affiliation: Institute for Frontier Medical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan. Electronic address: iwata@frontier.kyoto-u.ac.jp.
authors.forename: Hiroo
authors.initials: H
authors.lastname: Iwata
mesh_terms: D015415:Biomarkers / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D055096:Optical Devices; D019095:Point-of-Care Systems; D012031:Refractometry; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D015415:Biomarkers; D000509:alpha-Fetoproteins
keywords: Convergent optical system; Highly sensitive detection; Surface plasmon field-enhanced fluorescence spectroscopy; Tumor marker