title: Possibility of CTX-M-14 Gene Transfer from Shigella sonnei to a Commensal Escherichia coli Strain of the Gastroenteritis Microbiome.
abstract: OBJECTIVE
  To investigated whether the CTX-M-14 gene could be transferred from a clinical Shigella sonnei strain to commensal Escherichia coli strain in the gastroenteritis microbiome.
  
  
  METHODS
  E. coli strains were isolated from 30 stool samples of S. sonnei infected students in a gastroenteritis outbreak in 2004 and were characterized by antibiotic resistance analysis, in vitro conjugation and in vivo transfer of CTX-M-14 gene and molecular assays.
  
  
  RESULTS
  One strain of Escherichia coli that had high levels of resistance to cefotaxime was isolated from a patient infected with S. sonnei. Isoelectric focusing showed that the E. coli and S. sonnei strains produced a β-lactamase with an isoelectric point of 8.1. Moreover, polymerase chain reaction analysis indicated that both strains possessed the same DNA sequences for CTX-M-14. The results of in vitro and in vivo conjugation showed that the efficiency of CTX-M-14 transfer from S. sonnei to E. coli was similar to CTX-M-14 transfer between E. coli strains.
  
  
  CONCLUSIONS
  The data suggest that the acquisition of the extended-spectrum β-lactamases gene by pathogenic bacteria in the human intestinal tract to commensal microbiome bacteria can cause serious infectious diseases.
authors.affiliation: Division of Enteric Bacterial Infections, Korea National Institute of Health, Osong, Korea.
authors.forename: Seung-Hak
authors.initials: SH
authors.lastname: Cho
authors.affiliation: Division of Enteric Bacterial Infections, Korea National Institute of Health, Osong, Korea.
authors.forename: Soon Young
authors.initials: SY
authors.lastname: Han
authors.affiliation: Division of Enteric Bacterial Infections, Korea National Institute of Health, Osong, Korea.
authors.forename: Yeon-Ho
authors.initials: YH
authors.lastname: Kang
keywords: CTX-M-14; Gene transfer; Shigella sonnei; commensal Escherichia coli