title: Seed targeting with tiny anti-miR-155 inhibits malignant progression of multiple myeloma cells.
abstract: BACKGROUND
  miR-155 acts as a ubiquitous oncogene in major classes of human cancers and is a potential target for therapeutic intervention. However, the role of miR-155 in multiple myeloma is poorly understood.
  
  
  METHODS
  To explore the role of miR-155 in multiple myeloma, we assessed the influence of tiny seed-targeting anti-miR-155 (t-anti-miR-155) on multiple myeloma cell line (RPMI-8266) viability and apoptosis in vitro.
  
  
  RESULTS
  t-anti-miR-155 significantly inhibited multiple myeloma cell proliferation, migration, and colony formation. Additionally, t-anti-miR-155 significantly increased CD19 positive cell numbers, which are novel biomarkers for multiple myeloma and suppressor of cytokine signaling 1(SOCS1) was shown to be a target gene for miR-155 in multiple myeloma. Finally, the miR-155 signaling pathway was investigated by KEGG assay.
  
  
  CONCLUSIONS
  miR-155 in RPMI-8266 cells is a critical oncomiR in multiple myeloma and seed-targeting t-anti-miR-155 might be a novel strategy for miR-155-based therapeutics.
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College of Jinan University , Guangzhou , P.R. China.
authors.forename: Maoxiao
authors.initials: M
authors.lastname: Feng
authors.forename: Xiaochuang
authors.initials: X
authors.lastname: Luo
authors.forename: Chunming
authors.initials: C
authors.lastname: Gu
authors.forename: Jia
authors.initials: J
authors.lastname: Fei
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D054443:Cell Migration Assays; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D009101:Multiple Myeloma / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000096:biosynthesis / Q000187:drug effects; D014410:Tumor Stem Cell Assay
chemical_list: C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; C108944:SOCS1 protein, human; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins
keywords: miR-155; multiple myeloma; seed targeting; tiny anti-miRNA