title: Analgesic effect of intrathecal bumetanide is accompanied by changes in spinal sodium-potassium-chloride co-transporter 1 and potassium-chloride co-transporter 2 expression in a rat model of incisional pain.
abstract: Accumulating evidence has demonstrated that the sodium-potassium-chloride co-transporter 1 and potassium-chloride co-transporter 2 have a role in the modulation of pain transmission at the spinal level through chloride regulation in the pain pathway and by effecting neuronal excitability and pain sensitization. The present study aimed to investigate the analgesic effect of the specific sodium-potassium-chloride co-transporter 1 inhibitor bumetanide, and the change in spinal sodium-potassium-chloride co-transporter 1 and potassium-chloride co-transporter 2 expression in a rat model of incisional pain. Results showed that intrathecal bumetanide could decrease cumulative pain scores, and could increase thermal and mechanical pain thresholds in a rat model of incisional pain. Sodium-potassium-chloride co-transporter 1 expression increased in neurons from dorsal root ganglion and the deep laminae of the ipsilateral dorsal horn following incision. By contrast, potassium-chloride co-transporter 2 expression decreased in neurons of the deep laminae from the ipsilateral dorsal horn. These findings suggest that spinal sodium-potassium-chloride co-transporter 1 expression was up-regulated and spinal potassium-chloride co-transporter 2 expression was down-regulated following incision. Intrathecal bumetanide has analgesic effects on incisional pain through inhibition of sodium-potassium-chloride co-transporter 1.
authors.affiliation: Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China ; Department of Anesthesiology and Pain Medicine, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Yanbing
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Shiyuan
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Anesthesiology and Pain Medicine, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Anesthesiology and Pain Medicine, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Qingjuan
authors.initials: Q
authors.lastname: Gong
keywords: bumetanide; dorsal root ganglion; incision model; nerve regeneration; neural regeneration; postoperative pain; potassium-chloride co-transporter 2; sodium-potassium-chloride co-transporter 1; spinal cord