title: Effect of nicotine on melanogenesis and antioxidant status in HEMn-LP melanocytes.
abstract: Nicotine is a natural ingredient of tobacco plants and is responsible for the addictive properties of tobacco. Nowadays nicotine is also commonly used as a form of smoking cessation therapy. It is suggested that nicotine may be accumulated in human tissues containing melanin. This may in turn affect biochemical processes in human cells producing melanin. The aim of this study was to examine the effect of nicotine on melanogenesis and antioxidant status in cultured normal human melanocytes HEMn-LP. Nicotine induced concentration-dependent loss in melanocytes viability. The value of EC50 was determined to be 7.43 mM. Nicotine inhibited a melanization process in human light pigmented melanocytes and caused alterations of antioxidant defense system. Significant changes in cellular antioxidant enzymes: superoxide dismutase and catalase activities and in hydrogen peroxide content were stated. The obtained results may explain a potential influence of nicotine on biochemical processes in melanocytes in vivo during long term exposition to nicotine.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University of Silesia, Jagiellońska 4, 41-200 Sosnowiec, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Delijewski
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University of Silesia, Jagiellońska 4, 41-200 Sosnowiec, Poland.
authors.forename: Artur
authors.initials: A
authors.lastname: Beberok
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University of Silesia, Jagiellońska 4, 41-200 Sosnowiec, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Otręba
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University of Silesia, Jagiellońska 4, 41-200 Sosnowiec, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Wrześniok
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University of Silesia, Jagiellońska 4, 41-200 Sosnowiec, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Rok
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University of Silesia, Jagiellońska 4, 41-200 Sosnowiec, Poland. Electronic address: ebuszman@sum.edu.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Buszman
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D008543:Melanins / Q000096:biosynthesis*; D008544:Melanocytes / Q000187:drug effects* / Q000378:metabolism; D009538:Nicotine / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D008543:Melanins; D009538:Nicotine
keywords: Antioxidant enzymes; Melanin; Melanocytes; Nicotine; Tyrosinase