title: Intraspinal transplantation of motoneuron-like cell combined with delivery of polymer-based glial cell line-derived neurotrophic factor for repair of spinal cord contusion injury.
abstract: To evaluate the effects of glial cell line-derived neurotrophic factor transplantation combined with adipose-derived stem cells-transdifferentiated motoneuron delivery on spinal cord contusion injury, we developed rat models of spinal cord contusion injury, 7 days later, injected adipose-derived stem cells-transdifferentiated motoneurons into the epicenter, rostral and caudal regions of the impact site and simultaneously transplanted glial cell line-derived neurotrophic factor-gelfoam complex into the myelin sheath. Motoneuron-like cell transplantation combined with glial cell line-derived neurotrophic factor delivery reduced cavity formations and increased cell density in the transplantation site. The combined therapy exhibited superior promoting effects on recovery of motor function to transplantation of glial cell line-derived neurotrophic factor, adipose-derived stem cells or motoneurons alone. These findings suggest that motoneuron-like cell transplantation combined with glial cell line-derived neurotrophic factor delivery holds a great promise for repair of spinal cord injury.
authors.affiliation: Shefa Neuroscience Research Center at Khatam Al-Anbia Hospital, Tehran, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Abdanipour
authors.affiliation: Shefa Neuroscience Research Center at Khatam Al-Anbia Hospital, Tehran, Iran.
authors.forename: Taki
authors.initials: T
authors.lastname: Tiraihi
authors.affiliation: Shefa Neuroscience Research Center at Khatam Al-Anbia Hospital, Tehran, Iran.
authors.forename: Taher
authors.initials: T
authors.lastname: Taheri
keywords: adipose-derived stem cells; cell transplantation; glial cell line-derived neurotrophic factor; motoneurons; nerve regeneration; neural regeneration; neurotrophic factor; spinal cord contusion injury; spinal cord injury