title: Next generation sequencing yields the complete mitochondrial genome of the Endangered Chilean silverside Basilichthys microlepidotus (Jenyns, 1841) (Teleostei, Atherinopsidae), validated with RNA-seq.
abstract: The complete sequence of the mitochondrial genome for the Chilean silverside Basilichthys microlepidotus is reported for the first time. The entire mitochondrial genome was 16,544 bp in length (GenBank accession no. KM245937); gene composition and arrangement was conformed to that reported for most fishes and contained the typical structure of 2 rRNAs, 13 protein-coding genes, 22 tRNAs and a non-coding region. The assembled mitogenome was validated against sequences of COI and Control Region previously sequenced in our lab, functional genes from RNA-Seq data for the same species and the mitogenome of two other atherinopsid species available in Genbank.
authors.affiliation: a Departamento de Ciencias Ecológicas , Instituto de Ecología y Biodiversidad, Núcleo Milenio de Ecología y Manejo Sustentable de Islas Oceánicas, Universidad de Chile , Casilla 653, Ñuñoa , Santiago , Chile.
authors.forename: David
authors.initials: D
authors.lastname: Véliz
authors.affiliation: a Departamento de Ciencias Ecológicas , Instituto de Ecología y Biodiversidad, Núcleo Milenio de Ecología y Manejo Sustentable de Islas Oceánicas, Universidad de Chile , Casilla 653, Ñuñoa , Santiago , Chile.
authors.forename: Caren
authors.initials: C
authors.lastname: Vega-Retter
authors.affiliation: a Departamento de Ciencias Ecológicas , Instituto de Ecología y Biodiversidad, Núcleo Milenio de Ecología y Manejo Sustentable de Islas Oceánicas, Universidad de Chile , Casilla 653, Ñuñoa , Santiago , Chile.
authors.forename: Claudio
authors.initials: C
authors.lastname: Quezada-Romegialli
mesh_terms: D000818:Animals; D001482:Base Composition; D056727:Endangered Species; D005399:Fishes / Q000235:genetics*; D023061:Gene Order; D059646:Genome Size; D054629:Genome, Mitochondrial; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D008928:Mitochondria / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods; D017423:Sequence Analysis, RNA / Q000379:methods*
keywords: Freshwater fish; NGS; RNA-Seq; mitogenome