title: Alterations in miRNA expression patterns in whole blood of OSCC patients.
abstract: OBJECTIVE
  Aberrant microRNA (miRNA) expression in blood of cancer patients is a common finding. The present study aimed at evaluating the differences in miRNA expression in whole blood samples of oral squamous cell carcinoma (OSCC) patients compared to healthy controls.
  
  
  METHODS
  Microarray based miRNA profiling was performed on whole blood samples of 20 OSCC patients and 20 healthy volunteers and the differences in expression patterns between the two groups were evaluated. The results were validated by Reverse Transcription quantitative-Polymerase Chain Reaction (RT-qPCR) in 50 OSCC patients and 35 volunteers.
  
  
  RESULTS
  21 miRNAs were identified to be significantly differentially expressed in whole blood of OSCC patients compared to healthy controls. The impact of miR-186 (p=0.002), miR-494 (p=0.001) and miR-3651 (p=0.0001) could be validated by RT-qPCR.
  
  
  CONCLUSIONS
  The aberrant expressions of miR-186, miR-494 and miR-3651 in whole blood of OSCC patients may serve as the basis for establishing minimally-invasive screening methods for OSCC based on miRNAs as biomarkers.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Erlangen University Hospital, Erlangen, Germany jutta.ries@uk-erlangen.de.
authors.forename: Jutta
authors.initials: J
authors.lastname: Ries
authors.affiliation: Department of Oral and Maxillofacial Surgery, University of Athens Medical School, Athens, Greece.
authors.forename: Eleftherios
authors.initials: E
authors.lastname: Vairaktaris
authors.affiliation: Department of Oral and Maxillofacial Surgery, Erlangen University Hospital, Erlangen, Germany.
authors.forename: Rita
authors.initials: R
authors.lastname: Kintopp
authors.affiliation: Department of Oral and Maxillofacial Surgery, Erlangen University Hospital, Erlangen, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Baran
authors.affiliation: Department of Oral and Maxillofacial Surgery, Erlangen University Hospital, Erlangen, Germany.
authors.forename: Friedrich W
authors.initials: FW
authors.lastname: Neukam
authors.affiliation: Department of Oral and Maxillofacial Surgery, Halle University Hospital, Halle (Saale), Germany.
authors.forename: Emeka
authors.initials: E
authors.lastname: Nkenke
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019540:Area Under Curve; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D012372:ROC Curve; D059467:Transcriptome; D047368:Tumor Burden
chemical_list: D035683:MicroRNAs
keywords: OSCC; RT-qPCR; miRNA expression; microarray; screening method; whole blood