title: Remote control of tissue interactions via engineered photo-switchable cell surfaces.
abstract: We report a general cell surface molecular engineering strategy via liposome fusion delivery to create a dual photo-active and bio-orthogonal cell surface for remote controlled spatial and temporal manipulation of microtissue assembly and disassembly. Cell surface tailoring of chemoselective functional groups was achieved by a liposome fusion delivery method and quantified by flow cytometry and characterized by a new cell surface lipid pull down mass spectrometry strategy. Dynamic co-culture spheroid tissue assembly in solution and co-culture tissue multilayer assembly on materials was demonstrated by an intercellular photo-oxime ligation that could be remotely cleaved and disassembled on demand. Spatial and temporal control of microtissue structures containing multiple cell types was demonstrated by the generation of patterned multilayers for controlling stem cell differentiation. Remote control of cell interactions via cell surface engineering that allows for real-time manipulation of tissue dynamics may provide tools with the scope to answer fundamental questions of cell communication and initiate new biotechnologies ranging from imaging probes to drug delivery vehicles to regenerative medicine, inexpensive bioreactor technology and tissue engineering therapies.
authors.affiliation: 1] Department of Chemistry, Carolina Center for Genome Science, Carolina Center for Cancer Nanotechnology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA [2] Department of Chemistry and Biology, Centre for Research in Biomolecular Interactions, York University, Toronto, Ontario, M3J 1P3, Canada.
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.affiliation: Department of Chemistry, Carolina Center for Genome Science, Carolina Center for Cancer Nanotechnology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
authors.forename: Abigail
authors.initials: A
authors.lastname: Pulsipher
authors.affiliation: Department of Chemistry, Carolina Center for Genome Science, Carolina Center for Cancer Nanotechnology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
authors.forename: Debjit
authors.initials: D
authors.lastname: Dutta
authors.affiliation: Department of Chemistry, Carolina Center for Genome Science, Carolina Center for Cancer Nanotechnology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Lamb
authors.affiliation: 1] Department of Chemistry, Carolina Center for Genome Science, Carolina Center for Cancer Nanotechnology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA [2] Department of Chemistry and Biology, Centre for Research in Biomolecular Interactions, York University, Toronto, Ontario, M3J 1P3, Canada.
authors.forename: Muhammad N
authors.initials: MN
authors.lastname: Yousaf
mesh_terms: D002450:Cell Communication / Q000502:physiology; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D019169:Jurkat Cells; D008081:Liposomes / Q000378:metabolism*; D008164:Luminescent Proteins; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D044968:Regenerative Medicine / Q000379:methods; D023822:Tissue Engineering / Q000379:methods*; D000097573:Red Fluorescent Protein
chemical_list: D049452:Green Fluorescent Proteins; D008081:Liposomes; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein