title: Development and validation of an immunochromatographic assay for rapid detection of fumonisin B1 from cereal samples.
abstract: Fumonisins are one of the most agriculturally significant environmental toxins produced by Fusarium and Aspergillus species that grow on agricultural commodities in the field or during storage. Cereals contaminated with fumonisins causes serious loss to agricultural produce leads to health problems in humans and other farm animals. In the present study, polyclonal hyperimmune sera was raised against FB1 in rabbits immunized with FB1-keyhole limpet haemocyanin (KLH). Purified antibodies were used to establish a sensitive gold nanoparticle based immunochromatographic strip (ICG) for detecting FB1 levels in cereal grains. Effective on-site detection of FB1 was achieved by developing a rapid and sensitive pAb based ICG strip. This strip had a detection limit of 5 ng mL(-1) for FB1 in cereal samples and it could be completed within 3 min. Close examination of 150 cereal samples by ICG strip method revealed that 77 were fumonisin-positive. Results obtained by the developed method was further validated with well standardized HPLC method and results of strip method was correlated well with those obtained by HPLC method. In conclusion, the developed method was a better alternative for onsite detection of FB1 in cereal samples intended for human consumption to reduce risk of humans and other farm animals. The high level of FB1 concentrations recorded in present study warrants the need to develop an awareness creation programme to the farmers of India for safe handling of cereal grains at the time of harvesting and storage of grains.
authors.affiliation: DRDO-BU-Center for Life Sciences, Bharathiar University Campus, Coimbatore, Tamilnadu 641046 India ; DFRL, Mysore, 11 India.
authors.forename: M
authors.initials: M
authors.lastname: Venkataramana
authors.affiliation: Department of Applied Nutrition, Defence Food research Laboratory, Siddarthanagar, Mysore, 570011 Karnataka India.
authors.forename: K
authors.initials: K
authors.lastname: Navya
authors.affiliation: Department of Biotechnology, University of Mysore, Mysore, India.
authors.forename: S
authors.initials: S
authors.lastname: Chandranayaka
authors.affiliation: Defence Food Research Laboratory, Ministry of Defence, Siddarthanagar, Mysore, Karnataka 11 India.
authors.forename: S R
authors.initials: SR
authors.lastname: Priyanka
authors.affiliation: Defence Food Research Laboratory, Ministry of Defence, Siddarthanagar, Mysore, Karnataka 11 India.
authors.forename: H S
authors.initials: HS
authors.lastname: Murali
authors.affiliation: Defence Food Research Laboratory, Ministry of Defence, Siddarthanagar, Mysore, Karnataka 11 India.
authors.forename: H V
authors.initials: HV
authors.lastname: Batra
keywords: Antibodies; Cereal samples; Fumonisins; ICG strip