title: Chemical and Physical Cues Synergistically Affect Mating Behavior Sequences of Male Dasylepida ishigakiensis (Coleoptera: Scarabaeidae).
abstract: We investigated physical and chemical cues involved in male mating behavior of the white grub beetle, Dasylepida ishigakiensis (Scarabaeidae). When presented with female attractant pheromone (R)-2-butanol lures in a flight tunnel, nearly all males exhibited orientation and touching behaviors to freshly killed males and females and to intact glass models. Males landed and bent their abdomens on male and female bodies, but not on intact glass models. When treated with one female equivalent (FE) extract, washed immature male bodies and glass models both evoked stronger male responses than untreated equivalents, with the former eliciting a greater response than the treated glass models. Male responses to target male and female bodies decreased with increased numbers of washings of target bodies with organic solvents. These results suggest that the chemical factors that elicit male abdominal bending behavior are present on the body surface in both sexes. Washed immature male bodies treated with 1 FE or one male equivalent (ME) of extract induced strong male abdominal bending behavior. Washed mature female bodies treated with 1 ME extract also evoked male responses. Extracts of both sexes included factors eliciting male abdominal bending behavior. These results suggest that both physical and chemical cues derived from conspecifics cooperate to facilitate male mating recognition in D. ishigakiensis. The mating process of this species in the field is highly synchronized. Thus, after orienting to a female-like object, the only information males require by touching is whether the sex attractant pheromone that attracted them is indeed from a conspecific.
authors.affiliation: 1 Laboratory of Insect Interaction Research, National Institute of Agrobiological Sciences (NIAS), Ohwashi, Tsukuba, Ibaraki 305-0851, Japan.
authors.forename: Nao
authors.initials: N
authors.lastname: Fujiwara-Tsujii
authors.forename: Hiroe
authors.initials: H
authors.lastname: Yasui
authors.forename: Norio
authors.initials: N
authors.lastname: Arakaki
mesh_terms: D000818:Animals; D000440:Butanols / Q000494:pharmacology*; D001517:Coleoptera / Q000187:drug effects* / Q000502:physiology*; D003463:Cues; D005260:Female; D008297:Male; D010675:Pheromones / Q000494:pharmacology; D012726:Sexual Behavior, Animal / Q000187:drug effects*
chemical_list: D000440:Butanols; D010675:Pheromones; C043958:2-butanol
keywords: Dasylepida ishigakiensis; abdominal bending; contact pheromone; flight tunnel; male mating behavior