title: 660 nm red light-enhanced bone marrow mesenchymal stem cell transplantation for hypoxic-ischemic brain damage treatment.
abstract: Bone marrow mesenchymal stem cell transplantation is an effective treatment for neonatal hypoxic-ischemic brain damage. However, the in vivo transplantation effects are poor and their survival, colonization and differentiation efficiencies are relatively low. Red or near-infrared light from 600-1,000 nm promotes cellular migration and prevents apoptosis. Thus, we hypothesized that the combination of red light with bone marrow mesenchymal stem cell transplantation would be effective for the treatment of hypoxic-ischemic brain damage. In this study, the migration and colonization of cultured bone marrow mesenchymal stem cells on primary neurons after oxygen-glucose deprivation were detected using Transwell assay. The results showed that, after a 40-hour irradiation under red light-emitting diodes at 660 nm and 60 mW/cm(2), an increasing number of green fluorescence-labeled bone marrow mesenchymal stem cells migrated towards hypoxic-ischemic damaged primary neurons. Meanwhile, neonatal rats with hypoxic-ischemic brain damage were given an intraperitoneal injection of 1 × 10(6) bone marrow mesenchymal stem cells, followed by irradiation under red light-emitting diodes at 660 nm and 60 mW/cm(2) for 7 successive days. Shuttle box test results showed that, after phototherapy and bone marrow mesenchymal stem cell transplantation, the active avoidance response rate of hypoxic-ischemic brain damage rats was significantly increased, which was higher than that after bone marrow mesenchymal stem cell transplantation alone. Experimental findings indicate that 660 nm red light emitting diode irradiation promotes the migration of bone marrow mesenchymal stem cells, thereby enhancing the contribution of cell transplantation in the treatment of hypoxic-ischemic brain damage.
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.
authors.forename: Xianchao
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China ; Chongqing Engineering Research Center for Medical Electronics Technology, Chongqing, China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Hou
authors.affiliation: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China ; Chongqing Engineering Research Center for Medical Electronics Technology, Chongqing, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wu
authors.affiliation: Rehabilitation Center, Children's Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Chongqing Engineering Research Center for Medical Electronics Technology, Chongqing, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Chen
authors.affiliation: Rehabilitation Center, Children's Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Nong
authors.initials: N
authors.lastname: Xiao
authors.affiliation: Chongqing Engineering Research Center for Medical Electronics Technology, Chongqing, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
keywords: NSFC grant; Transwell assay; bone marrow mesenchymal stem cells; cell migration; hypoxic-ischemic brain damage; learning ability; nerve regeneration; neural regeneration; red light; stem cells; transplantation