title: Effect of periodontal therapy on C-reactive protein levels in gingival crevicular fluid of patients with gingivitis and chronic periodontitis: A clinical and biochemical study.
abstract: OBJECTIVE
  C-reactive protein (CRP) is a type I acute phase reactant. A number of studies have reported elevated serum CRP levels in periodontitis subjects, which decrease following periodontal therapy. However, the data of interventional studies on gingival crevicular fluid (GCF) levels of CRP is very scarce. The aim of the present study was to evaluate the effect of periodontal therapy on GCF CRP levels in patients with gingivitis and chronic periodontitis.
  
  
  METHODS
  A total of 60 subjects were included in the study with 20 subjects each in following groups: I-Healthy, II-Gingivitis, III-Mild periodontitis based on community periodontal index scores. Periodontal therapy was performed on Group II and Group III patients. GCF was collected from each subject at baseline and 3 months after periodontal therapy. The collected sample was subjected to biochemical analysis to detect CRP levels by using commercially available chemiluminescence immunoassay kit.
  
  
  RESULTS
  The present study demonstrated that the periodontitis group had a higher mean CRP level (2.49 ± 0.47 ng/ml) when compared with the Gingivitis group (1.40 ± 0.32 ng/ml) and Healthy group (0.56 ± 0.20 ng/ml). The mean CRP values after periodontal therapy were found to be reduced to 0.44 ± 0.23 ng/ml in Group II and 1.30 ± 0.36 ng/ml in Group III patients.
  
  
  CONCLUSIONS
  Within the limitations of this study, it can be concluded that GCF CRP level progressively increases from periodontal health to disease. It can also be stated that there is a decrease in GCF CRP levels with periodontal treatment.
authors.affiliation: Department of Periodontics, The Oxford Dental College and Hospital, Bengaluru, Karnataka, India.
authors.forename: Deepika
authors.initials: D
authors.lastname: Jayaprakash
authors.affiliation: Department of Periodontics, DAPMRV Dental College and Hospital, Bengaluru, Karnataka, India.
authors.forename: Suchetha
authors.initials: S
authors.lastname: Aghanashini
authors.affiliation: Department of Periodontics, DAPMRV Dental College and Hospital, Bengaluru, Karnataka, India.
authors.forename: Ranganatha Rao
authors.initials: RR
authors.lastname: Vijayendra
authors.affiliation: Department of Periodontics, The Oxford Dental College and Hospital, Bengaluru, Karnataka, India.
authors.forename: Anirban
authors.initials: A
authors.lastname: Chatterjee
authors.affiliation: Department of Periodontics, The Oxford Dental College and Hospital, Bengaluru, Karnataka, India.
authors.forename: Radhika Mohan
authors.initials: RM
authors.lastname: Rosh
authors.affiliation: Department of Periodontics, K.M. Shah Dental College, Vadodara, Gujarat, India.
authors.forename: Ashit
authors.initials: A
authors.lastname: Bharwani
keywords: C-reactive protein; Chronic periodontitis; enzyme immunoassay; gingival crevicular fluid; gingivitis