title: Immobilization of a thermostable, fungal recombinant chitinase on biocompatible chitosan beads and the properties of the immobilized enzyme.
abstract: A recombinant, thermostable fungal chitinase from the thermophilic fungus, Thermomyces lanuginosus, was immobilized on glutaraldehyde cross-linked chitosan beads, and the properties of the immobilized chitinase were studied. The enzyme was found to be almost completely immobilized in 6 H under shaking condition at 30 °C. The immobilized enzyme exhibited much wider pH optimum and was more stable at alkaline pH values as compared with the soluble enzyme. Both the forms of the enzyme were optimally active at 60 °C and stable at 50 °C for 3 H, and after 3 H, the activity of the soluble enzyme declined sharply, whereas the immobilized chitinase was stable up to 6 H without any significant loss in the activity. KM and Vmax values of the immobilized enzyme were 1.18 mM and 445.7 μmol/Min/mg of protein, respectively. The immobilized enzyme was stable at least for 1 month at 4 °C without any significant loss in the activity.
authors.affiliation: Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, India.
authors.forename: Muthu
authors.initials: M
authors.lastname: Prasad
authors.affiliation: Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, India.
authors.forename: Peramachi
authors.initials: P
authors.lastname: Palanivelu
mesh_terms: D001203:Ascomycota / Q000201:enzymology*; D002688:Chitinases / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D006358:Hot Temperature; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D011994:Recombinant Proteins; D048271:Chitosan; D002688:Chitinases
keywords: Thermomyces lanuginosus; chitooligosaccharides; chitosan beads; fungal recombinant chitinase; immobilization; thermophilic fungus