title: Individual and joint toxic effects of cadmium sulfate and α-naphthoflavone on the development of zebrafish embryo.
abstract: This paper aims to evaluate the individual and joint toxicities of cadmium sulfate (CdSO4) and α-naphthoflavone (ANF) in zebrafish embryos. As a result, CdSO4 caused both lethal and sub-lethal effects, such as 24 h post-fertilization (hpf) death and 72 hpf delayed hatching. However, ANF only caused sub-lethal effects, including 48 hpf cardiac edema and 72 hpf delayed hatching. Taking 24 hpf death and 48 hpf cardiac edema as endpoints, the toxicities of CdSO4 and ANF were significantly enhanced by each other. Consistently, both CdSO4 and ANF caused significant oxidative stress, including decreases in the reduced glutathione (GSH) level, inhibition of superoxide dismutase (SOD) activity, as well as increases in malondialdehyde (MDA) content in zebrafish embryos, but these mixtures produced much more significant alterations on the biomarkers. Co-treatment of CdSO4 and ANF significantly down-regulated the mRNA level of multidrug resistance-associated protein (mrp) 1 and cytochrome P450 (cyp) 1a, which constituted the protective mechanisms for zebrafish embryos to chemical toxins. In conclusion, co-treatment of CdSO4 and ANF exhibited a much more severe damage in zebrafish embryos than individual treatment. Meanwhile, production of oxidative stress and altered expression of mrp1 and cyp1a could be important components of such joint toxicity.
authors.affiliation: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academic of Sciences, Suzhou 215163, China; Suzhou Xiexin Photovoltaic Technology Co., Ltd., Suzhou 215163, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yin
authors.forename: Jian-ming
authors.initials: JM
authors.lastname: Yang
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Miao
authors.forename: Ying
authors.initials: Y
authors.lastname: Lin
authors.forename: Ming-li
authors.initials: ML
authors.lastname: Chen
mesh_terms: D000818:Animals; D001571:Benzoflavones / Q000008:administration & dosage / Q000633:toxicity*; D019187:Cadmium Compounds / Q000008:administration & dosage / Q000633:toxicity*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D004357:Drug Synergism; D015870:Gene Expression / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics; D018384:Oxidative Stress / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013431:Sulfates / Q000008:administration & dosage / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics
chemical_list: D001571:Benzoflavones; D019187:Cadmium Compounds; D003577:Cytochrome P-450 Enzyme System; D005978:Glutathione; D008315:Malondialdehyde; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D012333:RNA, Messenger; D013431:Sulfates; D013482:Superoxide Dismutase; D014874:Water Pollutants, Chemical; D029961:Zebrafish Proteins; C011512:alpha-naphthoflavone; C037123:cadmium sulfate; C421109:multidrug resistance-associated protein 1
keywords: Cadmium sulfate; Joint toxicity; Oxidative stress; Zebrafish embryo; α-Naphthoflavone