title: Simultaneous determination of taurine, glucuronolactone and glucuronic acid in energy drinks by ultra high performance liquid chromatography-tandem mass spectrometry (triple quadrupole).
abstract: In this work, we present for the first time a rapid and robust UHPLC-MS/MS method for analyzing taurine, GlcLA and GlcA in energy drinks simultaneously and without derivatization. The separation of three analytes was achieved using a Kinetex Hilic analytical column (100 mm × 4.6 mm i.d.) and a mobile phase formed by water (A) and acetonitrile (B) both with formic acid 0.1% at a flow rate of 0.8 ml min(-1) with isocratic elution in 3.5 min. Calibration curves were calculated using the method of standard addition in a concentration range from 2 to 6 mg/100 ml for taurine (R(2)>0.987), from 0.4 to 1.2 mg/100 ml for GlcLa (R(2)>0.997), and from 0.2 to 0.6 mg/100 ml for GlcA acid (R(2)>0.998). The validated method was applied to the analysis of nine commercial energy drinks. The level of taurine found ranged from 0.01 to 0.45 g/100 ml, and it matched with that reported in the labels of the analyzed energy drink samples.
authors.affiliation: HPLC-MS Laboratory, University of Camerino, Via Sant' Agostino 1, 62032, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Ricciutelli
authors.affiliation: School of Pharmacy, University of Camerino, Via Sant' Agostino 1, 62032, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Caprioli
authors.affiliation: HPLC-MS Laboratory, University of Camerino, Via Sant' Agostino 1, 62032, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Cortese
authors.affiliation: Servizio Polizia Scientifica, Gabinetto Interregionale Piemonte e Valle d'Aosta, Via Veglia 44, 10136 Torino, Italy.
authors.forename: Antonietta
authors.initials: A
authors.lastname: Lombardozzi
authors.affiliation: Servizio Polizia Scientifica, Gabinetto Interregionale Piemonte e Valle d'Aosta, Via Veglia 44, 10136 Torino, Italy.
authors.forename: Morela
authors.initials: M
authors.lastname: Strano
authors.affiliation: School of Pharmacy, University of Camerino, Via Sant' Agostino 1, 62032, Italy.
authors.forename: Sauro
authors.initials: S
authors.lastname: Vittori
authors.affiliation: School of Pharmacy, University of Camerino, Via Sant' Agostino 1, 62032, Italy. Electronic address: gianni.sagratini@unicam.it.
authors.forename: Gianni
authors.initials: G
authors.lastname: Sagratini
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D061215:Energy Drinks / Q000032:analysis*; D005965:Glucuronates / Q000032:analysis*; D020723:Glucuronic Acid / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods; D013654:Taurine / Q000032:analysis*
chemical_list: D005965:Glucuronates; D013654:Taurine; C004940:glucuronolactone; D020723:Glucuronic Acid
keywords: Energy drinks; Glucuronolactone; Taurine; UHPLC–MS/MS