title: Scutellaria baicalensis stem-leaf total flavonoid reduces neuronal apoptosis induced by amyloid beta-peptide (25-35).
abstract: Scutellaria baicalensis stem-leaf total flavonoid might attenuate learning/memory impairment and neuronal loss in rats induced by amyloid beta-peptide. This study aimed to explore the effects of Scutellaria baicalensis stem-leaf total flavonoid on amyloid beta-peptide-induced neuronal apoptosis and the expression of apoptosis-related proteins in the rat hippocampus. Male Wistar rats were given intragastric administration of Scutellaria baicalensis stem-leaf total flavonoid, 50 or 100 mg/kg, once per day. On day 8 after administration, 10 μg amyloid beta-peptide (25-35) was injected into the bilateral hippocampus of rats to induce neuronal apoptosis. On day 20, hippocampal tissue was harvested and probed with the terminal deoxyribonucleotidyl transferase-mediated biotin-16-dUTP nick-end labeling assay. Scutellaria baicalensis stem-leaf total flavonoid at 50 and 100 mg/kg reduced neuronal apoptosis induced by amyloid beta-peptide (25-35) in the rat hippocampus. Immunohistochemistry and western blot assay revealed that expression of the pro-apoptotic protein Bax, cytochrome c and caspase-3 was significantly diminished by 50 and 100 mg/kg Scutellaria baicalensis stem-leaf total flavonoid, while expression of the anti-apoptotic protein Bcl-2 was increased. Moreover, 100 mg/kg Scutellaria baicalensis stem-leaf total flavonoid had a more dramatic effect than the lower dosage. These experimental findings indicate that Scutellaria baicalensis stem-leaf total flavonoid dose-dependently attenuates neuronal apoptosis induced by amyloid beta-peptide in the hippocampus, and it might mediate this by regulating the expression of Bax, cytochrome c, caspase-3 and Bcl-2.
authors.affiliation: Department of Pharmacology, Chengde Medical College, Chengde 067000, Hebei Province, China.
authors.forename: Ruiting
authors.initials: R
authors.lastname: Wang
authors.affiliation: Affiliated Hospital of Chengde Medical College, Chengde 067000, Hebei Province, China.
authors.forename: Xingbin
authors.initials: X
authors.lastname: Shen
authors.affiliation: Affiliated Hospital of Chengde Medical College, Chengde 067000, Hebei Province, China.
authors.forename: Enhong
authors.initials: E
authors.lastname: Xing
authors.affiliation: Department of Pharmacology, Chengde Medical College, Chengde 067000, Hebei Province, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Guan
authors.affiliation: Affiliated Hospital of Chengde Medical College, Chengde 067000, Hebei Province, China.
authors.forename: Lisheng
authors.initials: L
authors.lastname: Xin
keywords: Alzheimer's disease; Scutellaria baicalensis stem-leaf total flavonoid; amyloid beta-peptide; apoptotic protein; cytochrome c; grants-supported paper; neural regeneration; neurodegenerative disease; neurons; neuroregeneration; traditional Chinese medicine