title: In vivo stem cell transplantation using reduced cell numbers.
abstract: Dental pulp stem cell (DPSC) characterization is essential for regeneration of a dentin/pulp like complex in vivo. This is especially important for identifying the potential of DPSCs to function as stem cells. Previously reported DPSC transplantation methods have used with huge numbers of cells, along with hydroxyapatite/tricalcium phosphate (HA/TCP), gelatin and fibrin, and collagen scaffolds. This protocol describe a transplantation protocol that uses fewer cells and a temperature-responsive cell culture dish.
authors.affiliation: Department of Pharmacology, School of Life Dentistry at Tokyo, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan, ryuryu@tokyo.ndu.ac.jp.
authors.forename: Takeo W
authors.initials: TW
authors.lastname: Tsutsui
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D003782:Dental Pulp / Q000166:cytology; D005260:Female; D051379:Mice; D061251:Primary Cell Culture / Q000379:methods; D012038:Regeneration; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology*