title: Male horse meiosis: metaphase I chromosome configuration and chiasmata distribution.
abstract: Chromosome configurations and chiasma frequency during the metaphase I stage of spermatogenesis in the male horse are characterized in this work. The genome-wide frequency and distribution of chiasmata was detected as 49.45 ± 2.07 for 14 fertile stallions. All X and Y chromosomes shared a single chiasma at their pseudoautosomal region, while 1-4 chiasmata were observed in autosomal chromosomes. The chiasma frequency and distribution were further studied for 8 different bivalents identified by FISH in 5 fertile stallions. Genetic length was calculated from chiasmata data for the whole genome as well as for these 8 chromosomes. The findings complement the genetic linkage data and provide insight into the genetic basis of spermatogenesis in normal stallions.
authors.affiliation: Molecular Biology and Genetics Laboratory, Central Veterinary Research Laboratory, Dubai, United Arab Emirates.
authors.forename: Ayman
authors.initials: A
authors.lastname: Al-Jaru
authors.forename: William
authors.initials: W
authors.lastname: Goodwin
authors.forename: Julian
authors.initials: J
authors.lastname: Skidmore
authors.forename: Terje
authors.initials: T
authors.lastname: Raudsepp
authors.forename: Kamal
authors.initials: K
authors.lastname: Khazanehdari
mesh_terms: D000818:Animals; D002369:Castration; D002467:Cell Nucleus / Q000502:physiology; D020090:Chromosome Segregation; D033481:Chromosomes, Mammalian / Q000235:genetics*; D003434:Crossing Over, Genetic; D006736:Horses / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008540:Meiosis; D008677:Metaphase; D012854:Sister Chromatid Exchange; D013091:Spermatogenesis