title: Patents in therapeutic recombinant protein production using mammalian cells.
abstract: The industrial production of recombinant proteins preferentially requires the generation of stable cell lines expressing proteins in a quick, relatively facile, and a reproducible manner. Different methods are used to insert exogenous DNA into the host cell, and choosing the appropriate producing cell is of paramount importance for the efficient production and quality of the recombinant protein. This review addresses the advances in recombinant protein production in mammalian cell lines, according to key patents from the last 30 years.
authors.forename: Virginia
authors.initials: V
authors.lastname: Picanco-Castro
authors.forename: Marcela Cristina Correa
authors.initials: MC
authors.lastname: de Freitas
authors.forename: Aline de Sousa
authors.initials: Ade S
authors.lastname: Bomfim
authors.affiliation: FCFRP-USP, DACTB, Av do Cafe s/n, Monte Alegre, CEP 14040-903, Ribeirao Preto, SP, Brazil. elisa@fcfrp.usp.br.
authors.forename: Elisa Maria
authors.initials: EM
authors.lastname: de Sousa Russo
mesh_terms: D000818:Animals; D000906:Antibodies / Q000235:genetics / Q000378:metabolism; D001779:Blood Coagulation Factors / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D005658:Fungi / Q000378:metabolism; D013006:Growth Hormone / Q000235:genetics / Q000378:metabolism; D006801:Humans; D010330:Patents as Topic; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000906:Antibodies; D001779:Blood Coagulation Factors; D003470:Culture Media; D011994:Recombinant Proteins; D013006:Growth Hormone