title: In silico mining of microsatellites in coding sequences of the date palm (Arecaceae) genome, characterization, and transferability.
abstract: OBJECTIVE
  To complement existing sets of primarily dinucleotide microsatellite loci from noncoding sequences of date palm, we developed primers for tri- and hexanucleotide microsatellite loci identified within genes. Due to their conserved genomic locations, the primers should be useful in other palm taxa, and their utility was tested in seven other Phoenix species and in Chamaerops, Livistona, and Hyphaene. •
  
  
  RESULTS
  Tandem repeat motifs of 3-6 bp were searched using a simple sequence repeat (SSR)-pipeline package in coding portions of the date palm draft genome sequence. Fifteen loci produced highly consistent amplification, intraspecific polymorphisms, and stepwise mutation patterns. •
  
  
  CONCLUSIONS
  These microsatellite loci showed sufficient levels of variability and transferability to make them useful for population genetic, selection signature, and interspecific gene flow studies in Phoenix and other Coryphoideae genera.
authors.affiliation: IRD, UMR DIADE-BDP, DYNADIV, and EVODYN teams, 911 Av. Agropolis, BP 64501, 34394 Montpellier, Cedex 5, France.
authors.forename: Frédérique
authors.initials: F
authors.lastname: Aberlenc-Bertossi
authors.affiliation: IRD, UMR DIADE-BDP, DYNADIV, and EVODYN teams, 911 Av. Agropolis, BP 64501, 34394 Montpellier, Cedex 5, France.
authors.forename: Karina
authors.initials: K
authors.lastname: Castillo
authors.affiliation: IRD, UMR DIADE-BDP, DYNADIV, and EVODYN teams, 911 Av. Agropolis, BP 64501, 34394 Montpellier, Cedex 5, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Tranchant-Dubreuil
authors.affiliation: IRD, UMR DIADE-BDP, DYNADIV, and EVODYN teams, 911 Av. Agropolis, BP 64501, 34394 Montpellier, Cedex 5, France ; Laboratoire de génétique moléculaire, immunologie et biotechnologie, Faculté des Sciences de Tunis, Campus Universitaire, 2092 El Manar, Tunis, Tunisia.
authors.forename: Emira
authors.initials: E
authors.lastname: Chérif
authors.affiliation: Consiglio per la ricerca e la sperimentazione in agricoltura-Unità di Ricerca per la Floricoltura e le Specie Ornamentali (CRA-FSO), Corso degli Inglesi 508, I-18038 Sanremo (IM), Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Ballardini
authors.affiliation: IRD, UMR DIADE-BDP, DYNADIV, and EVODYN teams, 911 Av. Agropolis, BP 64501, 34394 Montpellier, Cedex 5, France ; ISV/CERD, route de l'Aéroport, BP 468, Djibouti.
authors.forename: Sabira
authors.initials: S
authors.lastname: Abdoulkader
authors.affiliation: IRD, UMR DIADE-BDP, DYNADIV, and EVODYN teams, 911 Av. Agropolis, BP 64501, 34394 Montpellier, Cedex 5, France ; Centre de Bio-Archéologie et d'Ecologie (UMR 5059 CNRS/Université Montpellier 2/EPHE/INRAP), Institut de Botanique, 163 Rue Auguste Broussonet, 34090 Montpellier, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Gros-Balthazard
authors.affiliation: IRD, UMR DIADE-BDP, DYNADIV, and EVODYN teams, 911 Av. Agropolis, BP 64501, 34394 Montpellier, Cedex 5, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Chabrillange
authors.affiliation: INRA, UMR AGAP, 2 Place Viala, 34060 Montpellier, Cedex 1, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Santoni
authors.affiliation: Consiglio per la ricerca e la sperimentazione in agricoltura-Unità di Ricerca per la Floricoltura e le Specie Ornamentali (CRA-FSO), Corso degli Inglesi 508, I-18038 Sanremo (IM), Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Mercuri
authors.affiliation: IRD, UMR DIADE-BDP, DYNADIV, and EVODYN teams, 911 Av. Agropolis, BP 64501, 34394 Montpellier, Cedex 5, France.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Pintaud
keywords: Arecaceae; Coryphoideae; Phoenix dactylifera; microsatellite/SSR mining; transferability