title: Transient axonal glycoprotein-1 induces apoptosis-related gene expression without triggering apoptosis in U251 glioma cells.
abstract: Previous studies show that transient axonal glycoprotein-1, a ligand of amyloid precursor protein, increases the secretion of amyloid precursor protein intracellular domain and is involved in apoptosis in Alzheimer's disease. In this study, we examined the effects of transient axonal glycoprotein-1 on U251 glioma cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that transient axonal glycoprotein-1 did not inhibit the proliferation of U251 cells, but promoted cell viability. The terminal deoxynucleotidyl transferase dUTP nick end labeling assay showed that transient axonal glycoprotein-1 did not induce U251 cell apoptosis. Real-time PCR revealed that transient axonal glycoprotein-1 substantially upregulated levels of amyloid precursor protein intracellular C-terminal domain, and p53 and epidermal growth factor receptor mRNA expression. Thus, transient axonal glycoprotein-1 increased apoptosis-related gene expression in U251 cells without inducing apoptosis. Instead, transient axonal glycoprotein-1 promoted the proliferation of these glioma cells.
authors.affiliation: Department of Neurosurgery, the First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan Province, China ; Neurosurgery Institute, Key Laboratory on Brain Function Repair and Regeneration of Guangdong Province, Department of Neurosurgery, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Haigang
authors.initials: H
authors.lastname: Chang
authors.affiliation: Eight-year Programme, the First Clinical Medical College of Southern Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Song
authors.affiliation: Neurosurgery Institute, Key Laboratory on Brain Function Repair and Regeneration of Guangdong Province, Department of Neurosurgery, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Zhongcan
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, the First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan Province, China.
authors.forename: Yaxiao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Neurosurgery Institute, Key Laboratory on Brain Function Repair and Regeneration of Guangdong Province, Department of Neurosurgery, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Lujun
authors.initials: L
authors.lastname: Yang
authors.affiliation: Neurosurgery Institute, Key Laboratory on Brain Function Repair and Regeneration of Guangdong Province, Department of Neurosurgery, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Mouxuan
authors.initials: M
authors.lastname: Du
authors.affiliation: Neurosurgery Institute, Key Laboratory on Brain Function Repair and Regeneration of Guangdong Province, Department of Neurosurgery, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Yiquan
authors.initials: Y
authors.lastname: Ke
authors.affiliation: Department of Neurosurgery, Military General Hospital of Beijing PLA, Beijing, China.
authors.forename: Ruxiang
authors.initials: R
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, the First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan Province, China.
authors.forename: Baozhe
authors.initials: B
authors.lastname: Jin
authors.affiliation: Neurosurgery Institute, Key Laboratory on Brain Function Repair and Regeneration of Guangdong Province, Department of Neurosurgery, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Jiang
keywords: APP intracellular domain; NSFC grant; brain injury; epidermal growth factor receptor; glioma cells; nerve regeneration; neural regeneration; p53; transient axonal glycoprotein-1