title: Mitochondrial electron transport chain is involved in microcystin-RR induced tobacco BY-2 cells apoptosis.
abstract: Microcystin-RR (MC-RR) has been suggested to induce apoptosis in tobacco BY-2 cells through mitochondrial dysfunction including the loss of mitochondrial membrane potential (ΔΨm). To further elucidate the mechanisms involved in MC-RR induced apoptosis in tobacco BY-2 cells, we have investigated the role of mitochondrial electron transport chain (ETC) as a potential source for reactive oxygen species (ROS). Tobacco BY-2 cells after exposure to MC-RR (60mg/L) displayed apoptotic changes in association with an increased production of ROS and loss of ΔΨm. All of these adverse effects were significantly attenuated by ETC inhibitors including Rotenone (2μmol/L, complex I inhibitor) and antimycin A (0.01μmol/L, complex III inhibitor), but not by thenoyltrifluoroacetone (5μmol/L, complex II inhibitor). These results suggest that mitochondrial ETC plays a key role in mediating MC-RR induced apoptosis in tobacco BY-2 cells through an increased mitochondrial production of ROS.
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, the Chinese Academy of Sciences, Wuhan 430074, China. Electronic address: whuangwm@gmail.com.
authors.forename: Wenmin
authors.initials: W
authors.lastname: Huang
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. Electronic address: lidh@ihb.ac.cn.
authors.forename: Dunhai
authors.initials: D
authors.lastname: Li
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Yongding
authors.initials: Y
authors.lastname: Liu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D004579:Electron Transport / Q000187:drug effects*; D008387:Marine Toxins; D052998:Microcystins / Q000633:toxicity*; D008928:Mitochondria / Q000187:drug effects*; D014026:Nicotiana
chemical_list: D008387:Marine Toxins; D052998:Microcystins; C063855:microcystin RR
keywords: Apoptosis; Microcystin-RR; Mitochondrial electron transport chain; Reactive oxygen species; Tobacco BY-2 cells