title: Stereoscopic visualization and quantification of auricular cartilage regeneration in rabbits using multiphoton microscopy.
abstract: Multiphoton microscopy (MPM) was applied for imaging and quantifying the elastic cartilage regeneration tissue in a rabbit ear model without using labeling agents. Morphology of cells and collagen matrix were analysis, showing significant difference between regenerated and intact cartilage in cellular size and collagen distribution. The results demonstrate that high resolution images provide by MPM are consistent with the histological results, and show additional biological behavior which is not visible in standard histology. Advantages in instrumentation may lead to the application of MPM for intravital detection and treatment.
authors.affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, P. R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Zhu
authors.forename: Yahao
authors.initials: Y
authors.lastname: Xu
authors.forename: Yiyan
authors.initials: Y
authors.lastname: Tang
authors.forename: Shuyuan
authors.initials: S
authors.lastname: Xiong
authors.forename: Shuangmu
authors.initials: S
authors.lastname: Zhuo
authors.forename: Jianxin
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D004425:Ear Cartilage / Q000033:anatomy & histology* / Q000502:physiology*; D006651:Histocytochemistry; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D011817:Rabbits; D012038:Regeneration
keywords: auricular cartilage regeneration; multiphoton microscopy; quantification; three-dimensional imaging