title: Development and characterization of polymorphic microsatellite loci in Phellodendron amurense (Rutaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed for the rare species Phellodendron amurense to assess the genetic diversity and population structure of this plant. •
  
  
  RESULTS
  In total, 27 microsatellite markers were developed for P. amurense by using an enriched genomic library and hybridization; all of these primers successfully amplified DNA fragments in P. amurense. These markers were screened in 74 individuals from four populations in China; 15 loci were found to be polymorphic, with the number of alleles per locus ranging from one to nine. •
  
  
  CONCLUSIONS
  The microsatellite markers developed here represent a useful tool for studying the population genetic structure of P. amurense and to inform toward the development of effective conservation programs for this species.
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, Heilongjiang, People's Republic of China.
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Yu
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, Heilongjiang, People's Republic of China.
authors.forename: Chang-Mei
authors.initials: CM
authors.lastname: Chen
authors.affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, Liaoning, People's Republic of China.
authors.forename: Shi-Jie
authors.initials: SJ
authors.lastname: Han
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, Heilongjiang, People's Republic of China.
authors.forename: Xiao-Rui
authors.initials: XR
authors.lastname: Guo
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, Heilongjiang, People's Republic of China.
authors.forename: Shu-Sheng
authors.initials: SS
authors.lastname: Yuan
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, Heilongjiang, People's Republic of China.
authors.forename: Chun-Jing
authors.initials: CJ
authors.lastname: Wang
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, Heilongjiang, People's Republic of China.
authors.forename: Yuan-Gang
authors.initials: YG
authors.lastname: Zu
keywords: Phellodendron amurense; Rutaceae; genetic conservation; microsatellite marker; simple sequence repeat markers