title: Use of mep HyperCel for polishing of human serum albumin.
abstract: The manufacture of human serum albumin by chromatographic procedures involves gel filtration chromatography as a final polishing step. Despite this step being essential to remove high molecular weight impurity proteins and thus ensure a stable and safe final product, it is relatively inefficient. This paper explores the use of hydrophobic charge induction chromatographic media, MEP HyperCel as an alternative to Sephacryl S200HR gel filtration for the polishing of human serum albumin derived by ion exchange chromatographic purification of Cohn Supernatant I. The use of MEP HyperCel results in a product with a higher purity than achieved with gel filtration and in a less time consuming manner and with potential resource savings. MEP HyperCel appears to have great potential for incorporation into downstream processes in the plasma fractionation industry as an efficient means of achieving polishing of intermediates or capture of proteins of interest.
authors.affiliation: Research and Development Department, CSL Behring (Australia), 189-209 Camp Rd, Broadmeadows, Victoria 3047, Australia. Electronic address: karl.mccann@cslbehring.com.au.
authors.forename: Karl B
authors.initials: KB
authors.lastname: McCann
authors.affiliation: Research and Development Department, CSL Behring (Australia), 189-209 Camp Rd, Broadmeadows, Victoria 3047, Australia.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Vucica
authors.affiliation: Research and Development Department, CSL Behring (Australia), 189-209 Camp Rd, Broadmeadows, Victoria 3047, Australia.
authors.forename: John
authors.initials: J
authors.lastname: Wu
authors.affiliation: Research and Development Department, CSL Behring (Australia), 189-209 Camp Rd, Broadmeadows, Victoria 3047, Australia.
authors.forename: Joseph
authors.initials: J
authors.lastname: Bertolini
mesh_terms: D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D004340:Drug Contamination; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D012709:Serum Albumin / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000592:standards
chemical_list: D012709:Serum Albumin
keywords: Human serum albumin; Hydrophobic charge induction chromatography; MEP HyperCel; Plasma