title: A novel method of genomic DNA extraction for Cactaceae.
abstract: OBJECTIVE
  Genetic studies of Cactaceae can at times be impeded by difficult sampling logistics and/or high mucilage content in tissues. Simplifying sampling and DNA isolation through the use of cactus spines has not previously been investigated. •
  
  
  RESULTS
  Several protocols for extracting DNA from spines were tested and modified to maximize yield, amplification, and sequencing. Sampling of and extraction from spines resulted in a simplified protocol overall and complete avoidance of mucilage as compared to typical tissue extractions. Sequences from one nuclear and three plastid regions were obtained across eight genera and 20 species of cacti using DNA extracted from spines. •
  
  
  CONCLUSIONS
  Genomic DNA useful for amplification and sequencing can be obtained from cactus spines. The protocols described here are valuable for any cactus species, but are particularly useful for investigators interested in sampling living collections, extensive field sampling, and/or conservation genetic studies.
authors.affiliation: Research, Conservation, and Collections, Desert Botanical Garden, 1201 North Galvin Parkway, Phoenix, Arizona 85008 USA.
authors.forename: Shannon D
authors.initials: SD
authors.lastname: Fehlberg
authors.affiliation: Research, Conservation, and Collections, Desert Botanical Garden, 1201 North Galvin Parkway, Phoenix, Arizona 85008 USA.
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Allen
authors.affiliation: Research, Conservation, and Collections, Desert Botanical Garden, 1201 North Galvin Parkway, Phoenix, Arizona 85008 USA.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Church
keywords: DNA amplification; DNA extraction; DNA sequencing; cactus; cactus spines