title: Effects of low level laser therapy on attachment, proliferation, and gene expression of VEGF and VEGF receptor 2 of adipocyte-derived mesenchymal stem cells cultivated under nutritional deficiency.
abstract: Low-level laser therapy (LLLT) has been shown to increase the proliferation of several cell types. We evaluated the effects of LLLT on adhesion, proliferation, and gene expression of vascular endothelial growth factor (VEGF) and type 2 receptor of VEGF (VEGFR2) at mesenchymal stem cells (MSCs) from human (hMSCs) and rat (rMSCs) adipose tissues on nutritional deficiencies. A dose-response curve was performed with cells treated with laser Ga-Al-As (660 nm, 30 mW) at energy of 0.7 to 9 J. Cell adhesion and proliferation were quantified 20, 40, and 60 min after LLLT and 24, 72, and 120 h after cultivation. Gene expression was verified by RT-PCR after 2 h of LLLT. A minor nutritional support caused a significant decrease in proliferation and adhesion of hMSCs and rMSCs. However, at the lowest LLLT dose (0.7 J), we observed a higher proliferation in hMSCs at standard condition shortly after irradiation (24 h). Adhesion was higher in hMSCs cultivated in controlled conditions at higher LLLT doses (3 and 9 J), and rMSCs show a reduction in the adhesion on 1.5 to 9 J. On nutritional deprivation, a 9 J dose was shown to reduce proliferation with 24 h and adhesion to all culture times in rMSCs. VEGF and VEGFR2 were increased after LLLT in both cell types. However, hMSCs under nutritional deprivation showed higher expression of VEGF and its receptor after irradiation with other laser doses. In conclusion, LLLT on human and rat MSCs might upregulate VEGF messenger RNA (mRNA) expression and modulate cell adhesion and proliferation distinctively.
authors.affiliation: Nove de Julho University, Rua Vergueiro, 235, Liberdade, 01504-001, São Paulo, SP, Brazil.
authors.forename: Tabata Santos
authors.initials: TS
authors.lastname: de Oliveira
authors.forename: Andrey Jorge
authors.initials: AJ
authors.lastname: Serra
authors.forename: Martha Trindade
authors.initials: MT
authors.lastname: Manchini
authors.forename: Vinicius
authors.initials: V
authors.lastname: Bassaneze
authors.forename: José Eduardo
authors.initials: JE
authors.lastname: Krieger
authors.forename: Paulo
authors.initials: P
authors.lastname: de Tarso Camillo de Carvalho
authors.forename: Daniela Espindola
authors.initials: DE
authors.lastname: Antunes
authors.forename: Danilo Sales
authors.initials: DS
authors.lastname: Bocalini
authors.forename: Paulo José
authors.initials: PJ
authors.lastname: Ferreira Tucci
authors.forename: José Antônio
authors.initials: JA
authors.lastname: Silva
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology; D000818:Animals; D002448:Cell Adhesion / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects*; D002478:Cells, Cultured; D003470:Culture Media; D015870:Gene Expression / Q000528:radiation effects; D006801:Humans; D028022:Low-Level Light Therapy; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D051381:Rats; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics / Q000378:metabolism*
chemical_list: D003470:Culture Media; D042461:Vascular Endothelial Growth Factor A; D040301:Vascular Endothelial Growth Factor Receptor-2