title: Aptasensor based on tripetalous cadmium sulfide-graphene electrochemiluminescence for the detection of carcinoembryonic antigen.
abstract: A facile label-free electrochemiluminescence (ECL) aptasensor, based on the ECL of cadmium sulfide-graphene (CdS-GR) nanocomposites with peroxydisulfate as the coreactant, was designed for the detection of carcinoembryonic antigen (CEA). Tripetalous CdS-GR nanocomposites were synthesized through a simple onepot solvothermal method and immobilized on the glassy carbon electrode surface. L-Cystine (L-cys) could largely promote the electron transfer and enhance the ECL intensity. Gold nanoparticles (AuNPs) were assembled onto the L-cys film modified electrode for aptamer immobilization and ECL signal amplification. The aptamer modified with thiol was adsorbed onto the surface of the AuNPs through a Au-S bond. Upon hybridization of the aptamer with the target protein, the sequence could conjugate CEA to form a Y architecture. With CEA as a model analyte, the decreased ECL intensity is proportional to the CEA concentration in the range of 0.01-10.0 ng mL(-1) with a detection limit of 3.8 pg mL(-1) (S/N = 3). The prepared aptasensor was applied to the determination of CEA in human serum samples. The recoveries of CEA in the human serum samples were between 85.0% and 109.5%, and the RSD values were no more than 3.4%.
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China. liuym9518@sina.com.
authors.forename: Gui-Fang
authors.initials: GF
authors.lastname: Shi
authors.forename: Jun-Tao
authors.initials: JT
authors.lastname: Cao
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Zhang
authors.forename: Ke-Jing
authors.initials: KJ
authors.lastname: Huang
authors.forename: Yan-Ming
authors.initials: YM
authors.lastname: Liu
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Chen
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Ren
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D019187:Cadmium Compounds / Q000737:chemistry*; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D006108:Graphite / Q000737:chemistry*; D049449:Luminescence; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D013440:Sulfides / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D019187:Cadmium Compounds; D002272:Carcinoembryonic Antigen; D013440:Sulfides; C034939:cadmium sulfide; D006108:Graphite