title: Microsatellite loci for Orthophytum ophiuroides (Bromelioideae, Bromeliaceae) species adapted to neotropical rock outcrops.
abstract: OBJECTIVE
  Microsatellite primers were developed for Orthophytum ophiuroides, a rupicolous bromeliad species endemic to neotropical rocky fields. These microsatellite loci will be used to investigate population differentiation and species cohesion in such fragmented environments. The loci were tested for cross-amplification in related bromeliad species. •
  
  
  RESULTS
  Eleven polymorphic microsatellite markers were isolated and characterized from an enriched library of O. ophiuroides. The loci were tested on 42 individuals from two populations of this species. The number of alleles per locus ranged from three to nine and the expected and observed heterozygosities ranged from 0.167 to 0.870 and from 0.369 to 0.958, respectively. Seven loci successfully amplified in other related bromeliad species. •
  
  
  CONCLUSIONS
  Our results suggest that the microsatellite loci developed here will be useful to assess genetic diversity and gene flow in O. ophiuroides for the investigation of population differentiation and species cohesion in neotropical mountainous habitats.
authors.affiliation: Instituto de Botânica, 3687 Miguel Stéfano Avenue, 04301-902 São Paulo, São Paulo, Brazil.
authors.forename: Felipe
authors.initials: F
authors.lastname: Aoki-Gonçalves
authors.affiliation: Departamento de Botânica, Universidade Federal de Pernambuco, 1235 Prof. Moraes Rego Avenue, 50670-901 Recife, Pernambuco, Brazil.
authors.forename: Rafael B
authors.initials: RB
authors.lastname: Louzada
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, 400 Cândido Rondon Avenue, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Lívia Moura
authors.initials: LM
authors.lastname: De Souza
authors.affiliation: Instituto de Botânica, 3687 Miguel Stéfano Avenue, 04301-902 São Paulo, São Paulo, Brazil ; Departamento de Ecologia, Universidade Estadual Paulista Júlio de Mesquita Filho, 1514 24-A Avenue, 13506-900 Rio Claro, São Paulo, Brazil.
authors.forename: Clarisse
authors.initials: C
authors.lastname: Palma-Silva
keywords: Bromeliaceae; Orthophytum ophiuroides; cross-amplification; gene flow; genetic diversity; population genetics