title: Orthogonal design to sift the optimal parameter of Neiguan acupuncture for cerebral infarction.
abstract: The individual difference and non-repeatability in acupuncture have not only restricted the development of acupuncture, but have also affected the specificity of acupoints. The present study used instruments to control needle depth, lifting and thrusting frequency, and the duration of acupuncture. Effects of the quantified acupuncture were observed at Neiguan (PC6) with different stimulation parameters. A frequency of 1, 2, or 3 Hz and duration of 5, 60, or 180 seconds were used to observe cerebral blood flow and ratio of infarct volume recovery. Results showed that stimulation at Neiguan with a frequency of 1 Hz and long duration of 180 seconds or 2/3 Hz and long duration of 5/60 seconds significantly increased cerebral blood flow and decreased the ratio of infarct volume. Interactions between frequency and duration play a critical role in quantified acupuncture therapy.
authors.affiliation: First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Acupuncture and Moxibustion Research Institute of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China ; Key Laboratory of Cerebropathy Acupuncture Therapy of State Administration of Traditional Chinese Medicine, Tianjin 300193, China ; Tianjin Key Laboratory of Acupuncture & Moxibustion Science, Tianjin 300193, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Yang
authors.affiliation: Acupuncture and Moxibustion Research Institute of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China ; Key Laboratory of Cerebropathy Acupuncture Therapy of State Administration of Traditional Chinese Medicine, Tianjin 300193, China ; Tianjin Key Laboratory of Acupuncture & Moxibustion Science, Tianjin 300193, China ; Three-level Laboratory of Acupuncture Dose-Effect Relationship, State Administration of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Xiaonong
authors.initials: X
authors.lastname: Fan
authors.affiliation: Acupuncture and Moxibustion Research Institute of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China ; Key Laboratory of Cerebropathy Acupuncture Therapy of State Administration of Traditional Chinese Medicine, Tianjin 300193, China ; Tianjin Key Laboratory of Acupuncture & Moxibustion Science, Tianjin 300193, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Wuhai Hospital of Traditional Chinese Medicine, Wuhai 016000, Inner Mongolia Autonomous Region, China.
authors.forename: Nina
authors.initials: N
authors.lastname: He
authors.affiliation: Department of Rehabilitation Medicine, West China Medical School, West China Hospital Sichuan University, Chengdu 610041, Sichuan Province, China.
authors.forename: Lingxin
authors.initials: L
authors.lastname: Li
authors.affiliation: Postgraduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Luo
authors.affiliation: First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Xuemin
authors.initials: X
authors.lastname: Shi
keywords: Neiguan; acupoint; acupuncture duration; grants-supported paper; lifting and thrusting frequency; lifting and thrusting method; middle artery occlusion injury; neural regeneration; neuroregeneration; orthogonal design; quantified acupuncture