title: Impact of acute water and feed deprivation events on growth performance, intestinal characteristics, and serum stress markers in weaned pigs.
abstract: The impact of acute stressors (24-h feed or water deprivation) on growth performance, intestinal characteristics, and serum stress markers in weaned pigs was evaluated. Pigs (6.21 ± 0.29 kg) were allotted in a randomized complete block design to 4 treatments on the basis of BW at the time of weaning. There were 8 mixed-sex pigs in each of 12 pens per treatment. Treatments were arranged as a 2 × 2 factorial and consisted of a feed or water stressor that included a 0- or 24-h deprivation period postweaning, and pigs were subsequently allowed access to feed and water. Growth performance was measured 1, 7, 14, and 28 d postweaning. Serum and intestinal samples were taken 1 and 7 d postweaning. Serum was analyzed for cortisol and corticotrophin-releasing factor, and villus height, crypt depth, and mast cell density were measured in the jejunum and the ileum. Expression of mucin (MUC2), tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), claudin 1 (CL-1), occludin (OC), and zonula occludens 1 (ZO-1) genes were measured on d 1 and 7 postweaning in the jejunum and ileum by real-time PCR. There was a decrease (P < 0.05) in ADG with the water stressor 1 d postweaning, although subsequently, there were improvements (P < 0.05) in ADG and feed efficiency. Furthermore, the water stressor reduced ADFI during the last 14 d of the trial and cumulatively (P < 0.05). Seven days postweaning there was an increase (P < 0.05) in jejunal villous height to depth ratio due to the feed stressor and a decrease (P < 0.05) in the ileal villous height to depth ratio due to the water stressor. There was an increase (P < 0.05) in serum cortisol levels due to the water stressor both 1 and 7 d postweaning. Furthermore, there was an increase in serum corticotrophin-releasing factor 1 d but not 7 d postweaning due to the water stressor (P < 0.05). The feed stressor reduced (P < 0.05) TNF-α gene expression, and the water stressor reduced (P < 0.05) OC gene expression in the jejunum 1 d postweaning. In the ileum, there was a reduction in CL-1 and ZO-1 gene expression (P < 0.05) due to the water stressor 7 d postweaning. The results from the current investigation showed that a 24-h feed or water deprivation at the time of weaning has negative impacts on growth performance, intestinal characteristics, and serum stress responses immediately following the stress event and throughout the nursery period.
authors.affiliation: Department of Animal Sciences, Purdue University, West Lafayette, IN 47907.
authors.forename: N
authors.initials: N
authors.lastname: Horn
authors.affiliation: Enzyvia, LLC, Sheridan, IN 46069.
authors.forename: F
authors.initials: F
authors.lastname: Ruch
authors.affiliation: Biomatrix, Princeton, MN 55371.
authors.forename: G
authors.initials: G
authors.lastname: Miller
authors.affiliation: Department of Animal Sciences, Purdue University, West Lafayette, IN 47907.
authors.forename: K M
authors.initials: KM
authors.lastname: Ajuwon
authors.affiliation: Department of Animal Sciences, Purdue University, West Lafayette, IN 47907 ladeola@purdue.edu.
authors.forename: O
authors.initials: O
authors.lastname: Adeola
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood*; D001835:Body Weight; D003346:Corticotropin-Releasing Hormone / Q000097:blood; D005508:Food Deprivation / Q000502:physiology*; D006854:Hydrocortisone / Q000097:blood; D007413:Intestinal Mucosa / Q000254:growth & development; D007422:Intestines / Q000166:cytology / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013312:Stress, Physiological / Q000502:physiology*; D034421:Sus scrofa / Q000097:blood / Q000254:growth & development*; D013552:Swine; D013997:Time Factors; D014868:Water Deprivation / Q000502:physiology*; D014886:Weaning
chemical_list: D015415:Biomarkers; D003346:Corticotropin-Releasing Hormone; D006854:Hydrocortisone
keywords: acute stressors; nursery pig; serum stress markers; tight junction