title: Antimicrobial and anti-biofilm effect of a novel BODIPY photosensitizer against Pseudomonas aeruginosa PAO1.
abstract: Photodynamic therapy (PDT) combines the use of organic dyes (photosensitizers, PSs) and visible light in order to elicit a photo-oxidative stress which causes bacterial death. GD11, a recently synthesized PS belonging to the boron-dipyrromethene (BODIPY) class, was demonstrated to be efficient against planktonic cultures of Pseudomonas aeruginosa, causing a 7 log unit reduction of viable cells when administered at 2.5 μM. The effectiveness of GD11 against P. aeruginosa biofilms grown in flow-cells and microtiter trays was also demonstrated. Confocal laser scanning microscopy of flow-cell-grown biofilms suggests that the treatment has a biocidal effect against bacterial biofilm cells.
authors.affiliation: a Department of Theoretical and Applied Sciences , University of Insubria , Varese , Italy.
authors.forename: Viviana Teresa
authors.initials: VT
authors.lastname: Orlandi
authors.forename: Morten
authors.initials: M
authors.lastname: Rybtke
authors.forename: Enrico
authors.initials: E
authors.lastname: Caruso
authors.forename: Stefano
authors.initials: S
authors.lastname: Banfi
authors.forename: Tim
authors.initials: T
authors.lastname: Tolker-Nielsen
authors.forename: Paola
authors.initials: P
authors.lastname: Barbieri
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000528:radiation effects; D001895:Boron / Q000494:pharmacology; D018613:Microscopy, Confocal; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011162:Porphobilinogen / Q000031:analogs & derivatives / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology* / Q000528:radiation effects
chemical_list: D000900:Anti-Bacterial Agents; D017319:Photosensitizing Agents; C507544:dipyrromethene; D011162:Porphobilinogen; D001895:Boron
keywords: BODIPY; Pseudomonas aeruginosa; photodynamic treatment