title: Draft genome sequence of marine alphaproteobacterial strain HIMB11, the first cultivated representative of a unique lineage within the Roseobacter clade possessing an unusually small genome.
abstract: Strain HIMB11 is a planktonic marine bacterium isolated from coastal seawater in Kaneohe Bay, Oahu, Hawaii belonging to the ubiquitous and versatile Roseobacter clade of the alphaproteobacterial family Rhodobacteraceae. Here we describe the preliminary characteristics of strain HIMB11, including annotation of the draft genome sequence and comparative genomic analysis with other members of the Roseobacter lineage. The 3,098,747 bp draft genome is arranged in 34 contigs and contains 3,183 protein-coding genes and 54 RNA genes. Phylogenomic and 16S rRNA gene analyses indicate that HIMB11 represents a unique sublineage within the Roseobacter clade. Comparison with other publicly available genome sequences from members of the Roseobacter lineage reveals that strain HIMB11 has the genomic potential to utilize a wide variety of energy sources (e.g. organic matter, reduced inorganic sulfur, light, carbon monoxide), while possessing a reduced number of substrate transporters.
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Department of Microbiology, University of Georgia, Athens, Georgia, USA.
authors.forename: Bryndan P
authors.initials: BP
authors.lastname: Durham
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Department of Oceanography, University of Hawaii, Honolulu, Hawaii, USA.
authors.forename: Jana
authors.initials: J
authors.lastname: Grote
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Graduate School of Oceanography, University of Rhode Island, Narragansett, Rhode Island, USA.
authors.forename: Kerry A
authors.initials: KA
authors.lastname: Whittaker
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; School of Oceanography, University of Washington, Seattle, Washington, USA.
authors.forename: Sara J
authors.initials: SJ
authors.lastname: Bender
authors.affiliation: Department of Marine Sciences, University of Georgia, Athens, Georgia, USA.
authors.forename: Haiwei
authors.initials: H
authors.lastname: Luo
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Marine Biological Laboratory, Woods Hole, Massachusetts, USA.
authors.forename: Sharon L
authors.initials: SL
authors.lastname: Grim
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Department of Microbiology, Cornell University, Ithaca, New York, USA.
authors.forename: Julia M
authors.initials: JM
authors.lastname: Brown
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Department of Oceanography, University of Hawaii, Honolulu, Hawaii, USA.
authors.forename: John R
authors.initials: JR
authors.lastname: Casey
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Observatoire Océanologique de Villefranche, Villefranche-sur-mer, France.
authors.forename: Antony
authors.initials: A
authors.lastname: Dron
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Universidad Del Magdalena, Santa Marta, Colombia.
authors.forename: Lennin
authors.initials: L
authors.lastname: Florez-Leiva
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Max Plank Institute for Marine Microbiology, Bremen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Krupke
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Department of Ecology and Evolutionary Biology, Brown University, Providence, Rhode Island, USA.
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Luria
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Department of Geophysical Sciences, University of Chicago, Chicago, Illinois, USA.
authors.forename: Aric H
authors.initials: AH
authors.lastname: Mine
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Department of Oceanography, University of Hawaii, Honolulu, Hawaii, USA.
authors.forename: Olivia D
authors.initials: OD
authors.lastname: Nigro
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; School for Marine Science and Technology, University of Massachusetts Dartmouth, Dartmouth, Massachusetts, USA.
authors.forename: Santhiska
authors.initials: S
authors.lastname: Pather
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Department of Earth System Science, University of California Irvine, Irvine, CA, USA.
authors.forename: Agathe
authors.initials: A
authors.lastname: Talarmin
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Marine Science Institute, University of California Santa Barbara, Santa Barbara, California, USA.
authors.forename: Emma K
authors.initials: EK
authors.lastname: Wear
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Department of Atmospheric and Ocean Sciences, University of California Los Angeles, Los Angeles, California, USA.
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Weber
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; School of Natural Sciences, University of California Merced, Merced, California, USA.
authors.forename: Jesse M
authors.initials: JM
authors.lastname: Wilson
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Department of Oceanography, University of Hawaii, Honolulu, Hawaii, USA.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Church
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Edward F
authors.initials: EF
authors.lastname: DeLong
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Department of Oceanography, University of Hawaii, Honolulu, Hawaii, USA.
authors.forename: David M
authors.initials: DM
authors.lastname: Karl
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Department of Oceanography, University of Hawaii, Honolulu, Hawaii, USA.
authors.forename: Grieg F
authors.initials: GF
authors.lastname: Steward
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: John M
authors.initials: JM
authors.lastname: Eppley
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Department of Energy Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Nikos C
authors.initials: NC
authors.lastname: Kyrpides
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Singapore Centre on Environmental Life Sciences Engineering, Singapore.
authors.forename: Stephan
authors.initials: S
authors.lastname: Schuster
authors.affiliation: Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, Hawaii, USA ; Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe, Hawaii, USA.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Rappé
keywords: Roseobacter; aerobic anoxygenic phototroph; dimethylsulfoniopropionate; marine bacterioplankton