title: Diagnosis of gastrointestinal basidiobolomycosis: a mini-review.
abstract: Basidiobolus ranarum (Entomophthoromycotina) very rarely affects the gastrointestinal (GI) tract. To date, reported paediatric GI basidiobolomycosis cases are 27 worldwide; 19 from Saudi Arabia and 8 from other parts of the world. Often these cases present a diagnostic dilemma, are prone to misdiagnosis and lack of disease confirmation by proper molecular methodologies. The fungal mass removed by surgery is usually sent for conciliar histopathology, isolation by fungal cultures and final molecular testing for basidiobolomycosis. The incidence of basidiobolomycoses, their predisposing factors and the molecular diagnosis of the fungus causing the disease in combination with a phylogenetic framework are reviewed.
authors.affiliation: Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Mortada H
authors.initials: MH
authors.lastname: El-Shabrawi
authors.forename: Naglaa Mohamed
authors.initials: NM
authors.lastname: Kamal
authors.forename: Kerstin
authors.initials: K
authors.lastname: Kaerger
authors.forename: Kerstin
authors.initials: K
authors.lastname: Voigt
mesh_terms: D000666:Amphotericin B / Q000627:therapeutic use; D000935:Antifungal Agents / Q000627:therapeutic use; D053938:DNA Fragmentation; D004271:DNA, Fungal / Q000302:isolation & purification; D020091:Entomophthorales / Q000302:isolation & purification*; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006760:Hospitalization; D006801:Humans; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D020096:Zygomycosis / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D000666:Amphotericin B
keywords: 18S rRNA; Splendore-Hoeppli phenomenon; gastrointestinal basidiobolomycosis; identification; molecular typing