title: Simple and reliable urea assay based on a signal accumulation type of ion-sensitive field-effect transistor.
abstract: A simple urea assay was developed using a signal accumulation type of ion-sensitive field-effect transistor (SA-ISFET). Decreases in proton concentration resulting from urease-catalyzed hydrolysis of urea are detected by SA-ISFET as a change in potential. The method exhibits high sensitivity, linearity, and reproducibility when potential signals are accumulated 10-fold.
authors.affiliation: Kyoto Municipal Institute of Industrial Technology and Culture, 91 Chudouji Awata-cho, Shimogyo-ku, Kyoto 600-8813, Japan.
authors.forename: Naohiro
authors.initials: N
authors.lastname: Tomari
authors.affiliation: Department of Life Science, Faculty of Science and Engineering, Setsunan University, 17-8 Ikedanaka-machi, Neyagawa, Osaka 572-8508, Japan.
authors.forename: Asako
authors.initials: A
authors.lastname: Kawasaki
authors.affiliation: Kyoto Municipal Institute of Industrial Technology and Culture, 91 Chudouji Awata-cho, Shimogyo-ku, Kyoto 600-8813, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yamamoto
authors.affiliation: Department of Life Science, Faculty of Science and Engineering, Setsunan University, 17-8 Ikedanaka-machi, Neyagawa, Osaka 572-8508, Japan. Electronic address: nishiya@lif.setsunan.ac.jp.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Nishiya
mesh_terms: D055162:Biocatalysis; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007477:Ions; D011522:Protons; D015203:Reproducibility of Results; D014173:Transistors, Electronic; D014508:Urea / Q000032:analysis*; D014510:Urease / Q000378:metabolism
chemical_list: D007477:Ions; D011522:Protons; D014508:Urea; D014510:Urease
keywords: Ion-sensitive field-effect transistor; Proton; Signal accumulation; Urea assay; Urease