title: Preparation of bufalin-loaded pluronic polyetherimide nanoparticles, cellular uptake, distribution, and effect on colorectal cancer.
abstract: A large number of studies have shown that bufalin can have a significant antitumor effect in a variety of tumors. However, because of toxicity, insolubility in water, fast metabolism, short half-life, and other shortcomings, its application is limited in cancer therapy. In this study, we explored the anti-metastatic role of bufalin-loaded pluronic polyetherimide nanoparticles on HCT116 colon cancer-bearing mice. Nanoparticle size, shape, drug loading, encapsulation efficiency, and in vitro drug release were studied. Also, cellular uptake of nanoparticles, in vivo tumor targeting, and tumor metastasis were studied. The nanoparticles had a particle size of about 60 nm and an encapsulation efficiency of 75.71%, by weight. The in vitro release data showed that free bufalin was released faster than bufalin-loaded pluronic polyetherimide nanoparticles, and almost 80% of free bufalin was released after 32 hours. Nanoparticles had an even size distribution, were stable, and had a slow release and a tumor-targeting effect. Bufalin-loaded pluronic polyetherimide nanoparticles can significantly inhibit the growth and metastasis of colorectal cancer.
authors.affiliation: Department of Hepatobiliary Surgery, Qianfoshan Hospital, Shandong University, Jinan, People's Republic of China ; Department of General Surgery, Dahua Hospital, Shanghai, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Department of General Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of General Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China.
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Guo
authors.affiliation: Department of General Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China.
authors.forename: Yi-Jie
authors.initials: YJ
authors.lastname: Bao
authors.affiliation: Department of General Surgery, Dahua Hospital, Shanghai, People's Republic of China.
authors.forename: Dong-Hao
authors.initials: DH
authors.lastname: Xie
authors.affiliation: Department of General Surgery, Dahua Hospital, Shanghai, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: You-Rong
authors.initials: YR
authors.lastname: Duan
authors.affiliation: Department of General Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China.
authors.forename: Pei-Hao
authors.initials: PH
authors.lastname: Yin
authors.affiliation: Department of Hepatobiliary Surgery, Qianfoshan Hospital, Shandong University, Jinan, People's Republic of China.
authors.forename: Zhi-Hai
authors.initials: ZH
authors.lastname: Peng
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D002018:Bufanolides / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics; D045325:HCT116 Cells; D006801:Humans; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D020442:Poloxamer / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D002018:Bufanolides; D004337:Drug Carriers; D011108:Polymers; C433673:polyetherimide; D020442:Poloxamer; C022777:bufalin
keywords: bufalin; colon cancer; nanoparticles; tumor target