title: Coupled carbon, sulfur and nitrogen cycles of mixotrophic growth of Pseudomonas sp. C27 under denitrifying sulfide removal conditions.
abstract: Pseudomonas sp. C27 is a facultative autotrophic bacterium (FAB) that can effectively conduct mixotrophic denitrifying sulfide removal (DSR) reactions using organic matters and sulfide as electron donors. Quantitative proteomics analysis of C27 using isobaric tag for relative and absolute quantitation (iTRAQ) and bioinformatics techniques identified 1916 unique proteins, based on which a novel pathway utilizing couple carbon, sulfide and nitrogen cycles for mixotrophic growth of C27. DSR experiments at different C/N ratios confirmed the presence of the new pathway. This novel pathway may be of great significance for C27-alike strains to conduct sulfide and nitrate removals in biological treatments.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan; Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan. Electronic address: djlee@ntu.edu.tw.
authors.forename: Duu-Jong
authors.initials: DJ
authors.lastname: Lee
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China. Electronic address: waj0578@hit.edu.cn.
authors.forename: Aijie
authors.initials: A
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; School of Forestry, Northeast Forestry University, Harbin 150040, China.
authors.forename: Dawen
authors.initials: D
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Nanqi
authors.initials: N
authors.lastname: Ren
mesh_terms: D001483:Base Sequence; D001673:Biodegradation, Environmental; D057486:Carbon Cycle / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D019295:Computational Biology; D008969:Molecular Sequence Data; D058458:Nitrogen Cycle / Q000502:physiology*; D020543:Proteome / Q000235:genetics*; D040901:Proteomics; D011549:Pseudomonas / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013455:Sulfur / Q000378:metabolism*
chemical_list: D020543:Proteome; D012336:RNA, Ribosomal, 16S; D013455:Sulfur
keywords: Acetate; Pathway; Pseudomonas sp. C27; Sulfide