title: Reversal of experimentally induced seizure activity in mice by glibenclamide.
abstract: BACKGROUND
  ATP sensitive potassium channels are widely distributed in central nervous system (CNS) and these channels could be the target in CNS disorders by their modulators.
  
  
  OBJECTIVE
  The present study was designed to investigate the anticonvulsant potential of glibenclamide on MES induced seizure and pentylenetetrazole induced seizure in mice.
  
  
  METHODS
  Seizures were induced in 7 months albino mice with a single 12 mA intensity of 50 Hz stimulus for 0.2 s using electroconvulsiometer. Tonic flexion, tonic extension, clonic convulsion and mortality protection were recorded, 60 minutes after the oral administration of the vehicle (3% Tween 80), Standard (diazepam 3 mg/kg i.p.) and glibenclamide (5 mg/kg). In second model, seizures were induced with a single convulsive dose (80 mg/kg i.p) of pentylentetrazole (PTZ). Seizures were assessed in terms of onset of seizure, number of jerks, onset of tonic convulsion and clonic convulsions and mortality protection. The study was performed at antidiabetic dose of glibenclamide 5 mg/kg per oral.
  
  
  RESULTS
  Glibenclamide (5 mg/kg p.o.) showed significant (p<0.05) protective activity in MES induced seizures and attenuated pentylenetetrazole-induced seizure activity in mice. The anticonvulsant action of glibenclamide was noticeable in this study. However, further studies are required to elucidate its full anticonvulsant potential.
  
  
  CONCLUSIONS
  Glibenclamide is able to exert protective effects in MES induced seizures and attenuates pentylenetetrazole induced seizure activity in mice.
authors.affiliation: Department of Pharmacology, Parul Institute of Pharmacy, Vadodara.
authors.forename: Anand B
authors.initials: AB
authors.lastname: Pithadia
authors.affiliation: Department of Pharmacology, Parul Institute of Pharmacy, Vadodara.
authors.forename: Archana
authors.initials: A
authors.lastname: Navale
authors.affiliation: Department of Pharmacology, Parul Institute of Pharmacy, Vadodara.
authors.forename: Javed
authors.initials: J
authors.lastname: Mansuri
authors.affiliation: Department of Pharmacology, Parul Institute of Pharmacy, Vadodara.
authors.forename: Rajesh S
authors.initials: RS
authors.lastname: Shetty
authors.affiliation: Department of Pharmacology, Institute of Pharmacy, Nirma University, Ahmedabad.
authors.forename: Shital
authors.initials: S
authors.lastname: Panchal
authors.affiliation: Department of Pharmacology, L.M. College of Pharmacy, Ahmedabad, INDIA.
authors.forename: Sunita
authors.initials: S
authors.lastname: Goswami
keywords: GABA; Pentylenetetrazole; Potassium ion channels