title: Microsatellite markers for Leucobryum boninense (Leucobryaceae), endemic to the Bonin Islands, Japan.
abstract: OBJECTIVE
  Microsatellite primers were developed for Leucobryum boninense, endemic to the Bonin Islands, Japan, to investigate its level of genetic diversity and population genetic structure. •
  
  
  RESULTS
  Using next-generation sequencing, 21 primer sets were developed, among which nine loci were polymorphic in the populations of the Bonin Islands. Among these polymorphic loci, the number of alleles per locus ranged from two to 10 (mean = 3.444) and the expected heterozygosity ranged from 0.066 to 0.801 (mean = 0.338). •
  
  
  CONCLUSIONS
  These results indicate the utility of the nine microsatellite markers that we developed for population genetic studies of L. boninense.
authors.affiliation: Makino Herbarium, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo, 192-0397, Japan.
authors.forename: Emiko
authors.initials: E
authors.lastname: Oguri
authors.affiliation: Department of Biological Science, Graduate School of Science, Hiroshima University, 1-3-1 Kagamiyama, Higashi-hiroshima, Hiroshima, 739-8526, Japan.
authors.forename: Tomio
authors.initials: T
authors.lastname: Yamaguchi
authors.affiliation: Department of Biology, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba, 263-8522, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kajita
authors.affiliation: Makino Herbarium, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo, 192-0397, Japan.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Murakami
keywords: Bonin (Ogasawara) Islands; Leucobryaceae; Leucobryum boninense; Musci; microsatellite; next-generation sequencing