title: Analysis of nitrated proteins in Saccharomyces cerevisiae involved in mating signal transduction.
abstract: Protein tyrosine nitration (PTN) is a PTM that regulates signal transduction and inflammatory responses, and is related to neurodegenerative and cardiovascular diseases. The cellular function of PTN remains unclear because the low stoichiometry of PTN limits the identification and quantification of nitrated peptides. Effective enrichment is an important aspect of PTN analysis. In this study, we analyzed the in vivo nitroproteome elicited by mating signal transduction in Saccharomyces cerevisiae using a novel chemical enrichment method followed by LC-MS/MS. Nitroproteome profiling successfully identified changes in the nitration states of 14 proteins during mating signal transduction in S. cerevisiae, making this the first reported in vivo nitroproteome in yeast. We investigated the biological functions of these nitroproteins and their relationships to mating signal transduction in S. cerevisiae using a protein-protein interaction network. Our results suggest that PTN and denitration may be involved in nonreactive nitrogen species-mediated signal transduction and can provide clues for understanding the functional roles of PTN in vivo.
authors.affiliation: Department of Applied Chemistry, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea.
authors.forename: Jeong Won
authors.initials: JW
authors.lastname: Kang
authors.forename: Na Young
authors.initials: NY
authors.lastname: Lee
authors.forename: Kyung-Cho
authors.initials: KC
authors.lastname: Cho
authors.forename: Min Young
authors.initials: MY
authors.lastname: Lee
authors.forename: Do-Young
authors.initials: DY
authors.lastname: Choi
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Kwang Pyo
authors.initials: KP
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009566:Nitrates / Q000032:analysis* / Q000378:metabolism; D060066:Protein Interaction Maps; D011499:Protein Processing, Post-Translational; D040901:Proteomics; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction; D053719:Tandem Mass Spectrometry; D014443:Tyrosine / Q000032:analysis* / Q000378:metabolism
chemical_list: D009566:Nitrates; D029701:Saccharomyces cerevisiae Proteins; D014443:Tyrosine
keywords: Cell biology; LC-MS/MS; Mating signal transduction; Protein tyrosine nitration; Saccharomyces cerevisiae; Yeast