title: Upregulation of heat shock factor 1 transcription activity is associated with hepatocellular carcinoma progression.
abstract: Heat shock factor 1 (HSF1) is associated with tissue‑specific tumorigenesis in a number of mouse models, and has been used a as prognostic marker of cancer types, including breast and prostatic cancer. However, its role in human hepatocellular carcinoma (HCC) is not well understood. Using immunoblotting and immunohistochemical staining, it was identified that HSF1 and its serine (S) 326 phosphorylation, a biomarker of HSF1 activation, are significantly upregulated in human HCC tissues and HCC cell lines compared with their normal counterparts. Cohort analyses indicated that upregulation of the expression of HSF1 and its phospho‑S326 is significantly correlated with HCC progression, invasion and patient survival prognosis (P<0.001); however, not in the presence of a hepatitis B virus infection and the expression of alpha-fetoprotein and carcinoembryonic antigen. Knockdown of HSF1 with shRNA induced the protein expression of tumor suppressor retinoblastoma protein, resulting in attenuated plc/prf5 cell growth and colony formation in vitro. Taken together, these data markedly support that HSF1 is a potential prognostic marker and therapeutic target for the treatment of HCC.
authors.affiliation: State Key Laboratory of Antibody Engineering, Department of Genetics and Cell Biology, Henan University School of Medicine, Henan 475004, P.R. China.
authors.forename: Shulian
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Surgery, Huaihe Hospital Affiliated to Henan University, Kaifeng, Henan 475004, P.R. China.
authors.forename: Wanli
authors.initials: W
authors.lastname: Ma
authors.affiliation: Department of Surgery, Shanghai Tenth People's Hospital Affiliated to Tongji University, Shanghai 20072, P.R. China.
authors.forename: Teng
authors.initials: T
authors.lastname: Fei
authors.affiliation: State Key Laboratory of Antibody Engineering, Department of Genetics and Cell Biology, Henan University School of Medicine, Henan 475004, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lou
authors.affiliation: State Key Laboratory of Antibody Engineering, Department of Genetics and Cell Biology, Henan University School of Medicine, Henan 475004, P.R. China.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Antibody Engineering, Department of Genetics and Cell Biology, Henan University School of Medicine, Henan 475004, P.R. China.
authors.forename: Xiukun
authors.initials: X
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Antibody Engineering, Department of Genetics and Cell Biology, Henan University School of Medicine, Henan 475004, P.R. China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Antibody Engineering, Department of Genetics and Cell Biology, Henan University School of Medicine, Henan 475004, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Antibody Engineering, Department of Genetics and Cell Biology, Henan University School of Medicine, Henan 475004, P.R. China.
authors.forename: Guangchao
authors.initials: G
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Antibody Engineering, Department of Genetics and Cell Biology, Henan University School of Medicine, Henan 475004, P.R. China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Antibody Engineering, Department of Genetics and Cell Biology, Henan University School of Medicine, Henan 475004, P.R. China.
authors.forename: Yuanfang
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Surgery, Shanghai Tenth People's Hospital Affiliated to Tongji University, Shanghai 20072, P.R. China.
authors.forename: Zhengshun
authors.initials: Z
authors.lastname: Song
authors.affiliation: State Key Laboratory of Antibody Engineering, Department of Genetics and Cell Biology, Henan University School of Medicine, Henan 475004, P.R. China.
authors.forename: Yanzhong
authors.initials: Y
authors.lastname: Hu
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D000076249:Heat Shock Transcription Factors; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016160:Retinoblastoma Protein / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; C000619706:HSF1 protein, human; D018840:HSP70 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; D016160:Retinoblastoma Protein; D014157:Transcription Factors