title: STR DNA genotyping of hydatidiform moles in South China.
abstract: OBJECTIVE
  To evacuate whether short-tandem-repeat (STR) DNA genotyping is effective for diagnostic measure to precisely classify hydatidiform moles.
  
  
  METHODS
  150 cases were selected based on histologic features that were previously diagnosed or suspected molar pregnancy. All sections were stained with hematoxylin as a quality control method, and guided the microscopic dissection. DNA was extracted from dissected chorionic villi and paired maternal endometrial FFPE tissue sections. Then, STR DNA genotyping was performed by AmpFlSTR(®) Sinofiler(TM) PCR Amplification system (Applied Biosystems, Inc). Data collection and analysis were carried out using GeneMapper(®) ID-X version 1.2 (Applied Biosystems, Inc).
  
  
  RESULTS
  DNA genotyping was informative in all cases, leading to identification of 129 cases with abnormal genotype, including 95 complete and 34 partial moles, except 4 cases failed in PCR. Among 95 complete moles, 92 cases were monospermic and three were dispermic. Among 34 partial moles, 32 were dispermic and 2 were monospermic. The remaining 17 cases were balanced biallelic gestations.
  
  
  CONCLUSIONS
  STR DNA genotyping is effective for diagnostic measure to precisely classify hydatidiform moles. And in the absence of laser capture microdissection (LCM), hematoxylin staining plus manual dissection under microscopic guided is a more economic and practical method.
authors.affiliation: Department of Pathology, Peking University Health Science Center Beijing, China.
authors.forename: Xing-Zheng
authors.initials: XZ
authors.lastname: Zheng
authors.affiliation: Department of Pathology, Yale University School of Medicine New Haven, CT, USA.
authors.forename: Pei
authors.initials: P
authors.lastname: Hui
authors.affiliation: Department of Pathology, Fudan University Shanghai Cancer Center Shanghai, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Chang
authors.affiliation: Department of Pathology, Yurao People's Hospital Zhejiang, China.
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Gao
authors.affiliation: Department of Pathology, Peking University Health Science Center Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Pathology, Peking University Health Science Center Beijing, China.
authors.forename: Bing-Quan
authors.initials: BQ
authors.lastname: Wu
authors.affiliation: Department of Pathology, Peking University Health Science Center Beijing, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000328:Adult; D002681:China; D005260:Female; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D006828:Hydatidiform Mole / Q000145:classification* / Q000175:diagnosis / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000145:classification* / Q000175:diagnosis / Q000235:genetics; D055815:Young Adult
keywords: DNA genotyping; Short-tandem-repeat (STR); diagnosis; hydatidiform mole; non-molar gestation