title: Identification of an antigenic domain in the N-terminal region of avian hepatitis E virus (HEV) capsid protein that is not common to swine and human HEVs.
abstract: The antigenic domains located in the C-terminal 268 amino acid residues of avian hepatitis E virus (HEV) capsid protein have been characterized. This region shares common epitopes with swine and human HEVs. However, epitopes in the N-terminal 338 amino acid residues have never been reported. In this study, an antigenic domain located between amino acids 23 and 85 was identified by indirect ELISA using the truncated recombinant capsid proteins as coating antigens and anti-avian HEV chicken sera as primary antibodies. In addition, this domain did not react with anti-swine and human HEV sera. These results indicated that the N-terminal 338 amino acid residues of avian HEV capsid protein do not share common epitopes with swine and human HEVs. This finding is important for our understanding of the antigenicity of the avian HEV capsid protein. Furthermore, it has important implications in the selection of viral antigens for serological diagnosis.
authors.affiliation: Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of Agriculture, Yangling, Shaanxi 712100, PR China.|Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Lizhen
authors.initials: L
authors.lastname: Wang
authors.affiliation: Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of Agriculture, Yangling, Shaanxi 712100, PR China.|Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Yani
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of Agriculture, Yangling, Shaanxi 712100, PR China.|Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Taofeng
authors.initials: T
authors.lastname: Du
authors.affiliation: Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of Agriculture, Yangling, Shaanxi 712100, PR China.|Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Chengbao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of Agriculture, Yangling, Shaanxi 712100, PR China.|Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Shuqi
authors.initials: S
authors.lastname: Xiao
authors.affiliation: Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of Agriculture, Yangling, Shaanxi 712100, PR China.|Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Mu
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Henan Agriculture University, Zhengzhou, Henan 450002, PR China.|Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Gaiping
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Virology, Immunobiology and Parasitology, National Veterinary Institute (SVA), 75189 Uppsala, Sweden.
authors.forename: Lihong
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Virology, Immunobiology and Parasitology, National Veterinary Institute (SVA), 75189 Uppsala, Sweden.
authors.forename: Frederik
authors.initials: F
authors.lastname: Widén
authors.affiliation: Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
authors.forename: Walter H
authors.initials: WH
authors.lastname: Hsu
authors.affiliation: Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of Agriculture, Yangling, Shaanxi 712100, PR China.|Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, China Ministry of Agriculture, Yangling, Shaanxi 712100, PR China.|Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: En-Min
authors.initials: EM
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D002645:Chickens; D003429:Cross Reactions; D015967:Gene Expression Regulation, Viral; D016751:Hepatitis E / Q000662:veterinary / Q000821:virology; D016752:Hepatitis E virus / Q000235:genetics / Q000378:metabolism*; D006524:Hepatitis, Viral, Animal / Q000821:virology; D052678:Hepevirus / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D011201:Poultry Diseases / Q000821:virology*; D017434:Protein Structure, Tertiary; D012327:RNA Virus Infections / Q000662:veterinary / Q000821:virology; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000821:virology
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins