title: [Simultaneous determination of 15 polycyclic aromatic hydrocarbons additives in vegetable oil by ultra performance liquid chromatography].
abstract: OBJECTIVE
  To establish a rapid method for simultaneous determination of 15 polycyclic aromatic hydrocarbons (PAHs) in vegetable oil by ultra performance liquid chromatography with fluorescence detection.
  
  
  METHODS
  The vegetable oils were extracted with acetonitrile and acetone (1:1), purified with Oasis HLB and Sep-Pak Florsil column, separated on Waters PAH C18 (4.6 mm x 250 mm, 3 microm) special column for the analysis of polycyclic aromatic hydrocarbons with a mobile phase of acetonitrile, methanol and water for gradient elution, the column temperature was 35 degrees C, and the injection was 10 microl. The concentration of PAHs in samples were determined with fluorescence detector, and quantitative analysis was carried out by external standard.
  
  
  RESULTS
  The 15 PAHs were completely separated within 9 min. Within 2 to 200 microg/L, the peak area and content was in a good linear relationship (r > or = 0.9990). The average recoveries of three spiked levels (10, 50 and 100 microg/kg) were 75.8% and 96.4%, with RSDs of 3.42% - 8.03% (n = 5). The limits of detection were 0.025 - 0.8 microg/kg and the limits of quantification were 0.08 - 3.0 microg/kg.
  
  
  CONCLUSIONS
  This method is simple and quick with high sensitivity, and it is suitable for the determination of 15 PAHs in vegetable oil.
authors.forename: Guixian
authors.initials: G
authors.lastname: Dong
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Wang
authors.forename: Xiaoge
authors.initials: X
authors.lastname: Zhou
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Guifang
authors.initials: G
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D007202:Indicators and Reagents; D010938:Plant Oils; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000737:chemistry*; D014867:Water
chemical_list: D007202:Indicators and Reagents; D010938:Plant Oils; D011084:Polycyclic Aromatic Hydrocarbons; D014867:Water