title: Simultaneous determination of four neuroprotective compounds of Tilia amurensis by high performance liquid chromatography coupled with diode array detector.
abstract: BACKGROUND
  Tilia amurensis consists of various compounds, such as flavonoids and terpenoids.
  
  
  OBJECTIVE
  A simple and reliable high performance liquid chromatography (HPLC) coupled with the diode array detector (DAD) method has been established for simultaneous determination of epicatechin, nudiposide, lyoniside, and scopoletin isolated from Tilia amurensis.
  
  
  METHODS
  Optimum separations were obtained with a SHISEIDO C18 column by gradient eluton, with 0.1% Trifluoroacetic acid (TFA) water-methanol as the mobile phase. The gradient elution system was completed within 40 minutes. The flow rate and detection wavelength were 1 mL/minute, 205 nm, 250 nm, and 280 nm, respectively.
  
  
  RESULTS
  Validation of the analytical method was evaluated by linearity, precision, and the accuracy test. The calibration curve was linear over the established range with R(2) > 0.997. The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.01-15.20 μg/mL and 0.03-46.06 μg/mL. The method exhibited an intraday and interday precision range of 96.25-105.66% and 93.52-109.92%, respectively (RSD <2.80%). The recoveries and relative standard deviation (RSD) of the four compounds in Tilia amurensis were in the range of 90.42-104.84% and 0.2-2.58%.
  
  
  CONCLUSIONS
  This developed method was accurate and reliable for the quality evaluation of the four compounds isolated from Tilia amurensis.
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Chuncheon 200-701, Republic of Korea.
authors.forename: Bohyung
authors.initials: B
authors.lastname: Lee
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Chuncheon 200-701, Republic of Korea.
authors.forename: Jin Bae
authors.initials: JB
authors.lastname: Weon
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Chuncheon 200-701, Republic of Korea.
authors.forename: Bo-Ra
authors.initials: BR
authors.lastname: Yun
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Chuncheon 200-701, Republic of Korea.
authors.forename: Jiwoo
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Chuncheon 200-701, Republic of Korea.
authors.forename: Min Rye
authors.initials: MR
authors.lastname: Eom
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science, Chuncheon 200-701, Republic of Korea ; Research Institute of Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Choong Je
authors.initials: CJ
authors.lastname: Ma
keywords: High performance liquid chromatography/diode array detector; Tilia amurensis; quality control; simultaneous determination