title: Genome sequence of the acid-tolerant Burkholderia sp. strain WSM2232 from Karijini National Park, Australia.
abstract: Burkholderia sp. strain WSM2232 is an aerobic, motile, Gram-negative, non-spore-forming acid-tolerant rod that was trapped in 2001 from acidic soil collected from Karijini National Park (Australia) using Gastrolobium capitatum as a host. WSM2232 was effective in nitrogen fixation with G. capitatum but subsequently lost symbiotic competence during long-term storage. Here we describe the features of Burkholderia sp. strain WSM2232, together with genome sequence information and its annotation. The 7,208,311 bp standard-draft genome is arranged into 72 scaffolds of 72 contigs containing 6,322 protein-coding genes and 61 RNA-only encoding genes. The loss of symbiotic capability can now be attributed to the loss of nodulation and nitrogen fixation genes from the genome. This rhizobial genome is one of 100 sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.
authors.affiliation: School of Biomedical Sciences, Faculty of Health Sciences, Curtin University, Western Australia, Australia.
authors.forename: Robert
authors.initials: R
authors.lastname: Walker
authors.affiliation: School of Biomedical Sciences, Faculty of Health Sciences, Curtin University, Western Australia, Australia.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Watkin
authors.affiliation: Centre for Rhizobium Studies, School of Veterinary and Life Sciences, Murdoch University, Western Australia, Australia.
authors.forename: Rui
authors.initials: R
authors.lastname: Tian
authors.affiliation: School of Life and Environmental Sciences, Deakin University, Victoria, Australia.
authors.forename: Lambert
authors.initials: L
authors.lastname: Bräu
authors.affiliation: Centre for Rhizobium Studies, School of Veterinary and Life Sciences, Murdoch University, Western Australia, Australia.
authors.forename: Graham
authors.initials: G
authors.lastname: O'Hara
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors.forename: Lynne
authors.initials: L
authors.lastname: Goodwin
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: James
authors.initials: J
authors.lastname: Han
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Tatiparthi
authors.initials: T
authors.lastname: Reddy
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Marcel
authors.initials: M
authors.lastname: Huntemann
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Amrita
authors.initials: A
authors.lastname: Pati
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Tanja
authors.initials: T
authors.lastname: Woyke
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Mavromatis
authors.affiliation: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Victor
authors.initials: V
authors.lastname: Markowitz
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Ivanova
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Nikos
authors.initials: N
authors.lastname: Kyrpides
authors.affiliation: Centre for Rhizobium Studies, School of Veterinary and Life Sciences, Murdoch University, Western Australia, Australia.
authors.forename: Wayne
authors.initials: W
authors.lastname: Reeve
keywords: Betaproteobacteria; nitrogen fixation; rhizobia; root-nodule bacteria