title: Development of a novel in vivo cancer model using cell sheet engineering.
abstract: OBJECTIVE
  Standard in vivo cancer models entail injecting single cancer cells, but this technique is not always successful. We developed a novel cancer cell sheet by using temperature-responsive polymer poly(N-isopropyl acryl amide)-coated plates, which allow controlled attachment and detachment of living cancer cells via simple temperature changes.
  
  
  METHODS
  Four human cancer cell lines were used to make cell sheets. The cancer cell sheets were subcutaneously transplanted into nude mice and compared regarding their tumor-forming ability with the conventional cell suspension technique.
  
  
  RESULTS
  Human cancer cell sheets were successfully transplanted into nude mice. The cancer cell sheets resulted in stable engraftment and showed a higher tumor volume determined by total flux with the IVIS® imaging system.
  
  
  CONCLUSIONS
  Novel cancer cell sheets are useful tools to make in vivo cancer models in mice for the assessment of anticancer therapeutics.
authors.affiliation: Department of Gastroenterological Surgery, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Suzuki
authors.affiliation: Department of Gastroenterological Surgery, Tokyo Women's Medical University, Tokyo, Japan Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan aruga.atsushi@twmu.ac.jp.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Aruga
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Kobayashi
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.affiliation: Department of Gastroenterological Surgery, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D045325:HCT116 Cells; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
keywords: Cancer; cell sheet; mouse model; tissue engineering; transplantation