title: VEGF-releasing suture material for enhancement of vascularization: development, in vitro and in vivo study.
abstract: As it has been demonstrated that bioactive substances can be delivered locally using coated surgical suture materials, the authors developed a vascular endothelial growth factor (VEGF)-releasing suture material that should promote vascularization and potentially wound healing. In this context, the study focused on the characterization of the developed suture material and the verification of its biological activity, as well as establishing a coating process that allows reproducible and stable coating of a commercially available polydioxanone suture material with poly(l-lactide) (PLLA) and 0.1μg and 1.0μg VEGF. The in vitro VEGF release kinetics was studied using a Sandwich ELISA. The biological activity of the released VEGF was investigated in vitro using human umbilical vein endothelial cells. The potential of the VEGF-releasing suture material was also studied in vivo 5days after implantation in the hind limb of Wistar rats, when the histological findings were analyzed. The essential results, enhanced cell viability in vitro as well as significantly increased vascularization in vivo, were achieved using PLLA/1.0μg VEGF-coated suture material. Furthermore, ELISA measurements revealed a high reproducibility of the VEGF release behavior. Based on the results achieved regarding the dose-effect relationship of VEGF, the stability during its processing and the release behavior, it can be predicted that a bioactive suture material would be successful in later in vivo studies. Therefore, this knowledge could be the basis for future studies, where bioactive substances with different modes of action are combined for targeted, overall enhancement of wound healing.
authors.affiliation: Department of General, Thoracic, Vascular and Transplantation Surgery, University of Rostock, Schillingallee 35, D-18057 Rostock, Germany. Electronic address: christian.bigalke@googlemail.com.
authors.forename: Christian
authors.initials: C
authors.lastname: Bigalke
authors.affiliation: Institute for Biomedical Engineering, University of Rostock, Friedrich-Barnewitz-Str. 4, D-18119 Rostock, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Luderer
authors.affiliation: Institute for Biomedical Engineering, University of Rostock, Friedrich-Barnewitz-Str. 4, D-18119 Rostock, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Wulf
authors.affiliation: Institute for Biomedical Engineering, University of Rostock, Friedrich-Barnewitz-Str. 4, D-18119 Rostock, Germany.
authors.forename: Thilo
authors.initials: T
authors.lastname: Storm
authors.affiliation: Institute for Biomedical Engineering, University of Rostock, Friedrich-Barnewitz-Str. 4, D-18119 Rostock, Germany.
authors.forename: Marian
authors.initials: M
authors.lastname: Löbler
authors.affiliation: Institute for Biomedical Engineering, University of Rostock, Friedrich-Barnewitz-Str. 4, D-18119 Rostock, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Arbeiter
authors.affiliation: Department of General, Thoracic, Vascular and Transplantation Surgery, University of Rostock, Schillingallee 35, D-18057 Rostock, Germany.
authors.forename: Bettina M
authors.initials: BM
authors.lastname: Rau
authors.affiliation: Institute for Biomedical Engineering, University of Rostock, Friedrich-Barnewitz-Str. 4, D-18119 Rostock, Germany; Institute of Pathology, University of Rostock, Strempelstr. 14, D-18055 Rostock, Germany.
authors.forename: Horst
authors.initials: H
authors.lastname: Nizze
authors.affiliation: Institute for Experimental Surgery, University of Rostock, Schillingallee 69a, D-18057 Rostock, Germany.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Vollmar
authors.affiliation: Institute for Biomedical Engineering, University of Rostock, Friedrich-Barnewitz-Str. 4, D-18119 Rostock, Germany.
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Schmitz
authors.affiliation: Department of General, Thoracic, Vascular and Transplantation Surgery, University of Rostock, Schillingallee 35, D-18057 Rostock, Germany.
authors.forename: Ernst
authors.initials: E
authors.lastname: Klar
authors.affiliation: Institute for Biomedical Engineering, University of Rostock, Friedrich-Barnewitz-Str. 4, D-18119 Rostock, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Sternberg
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000008:administration & dosage* / Q000737:chemistry; D000818:Animals; D001808:Blood Vessels / Q000187:drug effects / Q000254:growth & development*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004058:Diffusion; D004343:Drug Implants / Q000008:administration & dosage* / Q000737:chemistry; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D018919:Neovascularization, Physiologic / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013537:Sutures; D016896:Treatment Outcome; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D043925:Angiogenesis Inducing Agents; D004343:Drug Implants; D042461:Vascular Endothelial Growth Factor A
keywords: Coating; Suture material; Vascular endothelial growth factor; Vascularization