title: Highly fluorescent amidine/schiff base dual-modified polyacrylonitrile nanoparticles for selective and sensitive detection of copper ions in living cells.
abstract: Highly fluorescent surface modified polyacrylonitrile nanoparticles (PAN NPs) of 50 nm diameter were fabricated for selective Cu(2+) sensing. After surface modification, the PAN NPs were converted to amidine/Schiff base dual-modified PAN nanoparticles (tPAN NPs) with a Cu(2+) sensing property and high QY (0.19). The selectivity of tPAN NPs for Cu(2+) is much higher than that of other metal ions due to the fact that amidine group on the surface of tPAN NPs has a higher binding affinity with Cu(2+). The effect of other metal ions on the fluorescence intensity of the tPAN NPs was also studied, and other metal ions showed a low interference response in the detection of Cu(2+). Furthermore, as a metal ion chelator, ethylenediaminetetraacetate can competitively interact with Cu(2+) to recover the quenched fluorescence of tPAN NPs. The tPAN NPs are easily introduced into cells and exhibit low toxicity, enabling their use as a fluorescence sensor for Cu(2+) in living cells. The tPAN NPs provide a new direction for the development of copper ion sensors in living cells.
authors.affiliation: WCU Program of Chemical Convergence for Energy and Environment (C2E2), School of Chemical and Biological Engineering, College of Engineering, Seoul National University , Shinlimdong 56-1, Seoul 151-742, Korea.
authors.forename: Inkyu
authors.initials: I
authors.lastname: Lee
authors.forename: Sojin
authors.initials: S
authors.lastname: Kim
authors.forename: Seh-Na
authors.initials: SN
authors.lastname: Kim
authors.forename: Yoonsun
authors.initials: Y
authors.lastname: Jang
authors.forename: Jyongsik
authors.initials: J
authors.lastname: Jang
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000578:Amidines / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival; D003300:Copper / Q000032:analysis*; D005453:Fluorescence; D006801:Humans; D007477:Ions; D008857:Microscopy, Interference; D053758:Nanoparticles / Q000648:ultrastructure; D010316:Particle Size; D012545:Schiff Bases / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000180:Acrylic Resins; D000578:Amidines; D007477:Ions; D012545:Schiff Bases; C010504:polyacrylonitrile; D003300:Copper
keywords: cell imaging; copper ion; fluorescence; metal ion sensing; polymer nanoparticle