title: Expression and function of natural antisense transcripts in mouse embryonic stem cells.
abstract: Non-coding RNAs (ncRNAs), such as microRNAs and large intergenic non-coding RNAs, have been shown to play essential roles in regulating pluripotency. Yet, it is not clear the role of natural antisense transcripts (NATs), also belonging to ncRNAs, in embryonic stem cells. However, the role of NATs in embryonic stem cells remains unknown. We further confirmed the expression of the NATs of three key pluripotency genes, Oct4, Nanog and Sox2. Moreover, overexpression of Sox2-NAT reduces the expression of Sox2 protein, and slightly enhances the Sox2 mRNA level. Altogether, our data indicated that like other ncRNAs, NATs might be involved in pluripotency maintenance.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Collaborative Innovation Center for Biotherapy, 2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics and College of Life Sciences, Nankai University, Tianjin, 300071, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhao
authors.forename: ZhaoTing
authors.initials: Z
authors.lastname: Wu
authors.forename: SiYu
authors.initials: S
authors.lastname: Wang
authors.forename: LingYi
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D053595:Embryonic Stem Cells / Q000378:metabolism*; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D016372:RNA, Antisense / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000502:physiology*; D055748:SOXB1 Transcription Factors / Q000235:genetics
chemical_list: D017931:DNA Primers; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D016372:RNA, Antisense; D012333:RNA, Messenger; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse