title: Visfatin and oxidative stress influence endothelial progenitor cells in obese populations.
abstract: PURPOSE/AIMS OF THE STUDY: This study sought to study the levels of circulating endothelial progenitor cells (EPCs), serum visfatin, and oxidative stress in obese individuals, and their respective correlations.
  
  
  METHODS
  The circulating levels of EPCs were measured through detecting CD309 and CD34 by flow cytometry. Serum visfatin concentration was measured by enzyme-linked immunosorbent assay in 31 obese men [obese group: BMI 28.9 ± 0.86 kg/m(2); age 44.93 ± 1.78 years (range 40 to 47)] and 30 normal-weight men [control group: BMI 22.7 ± 1.22 kg/m(2); age 44.03 ± 1.87 years (range 41 to 47)]. Indexes of oxidative stress were assayed by a colorimetric method. The relationships between circulating EPCs, serum visfatin, and oxidative stress markers were further analyzed by multiple linear regression analysis. Statistical significance was set at p < 0.05.
  
  
  RESULTS
  The obese group showed higher levels of serum visfatin and a lower level of circulating EPCs compared with controls. Serum superoxide dismutase (SOD) activity, total antioxidant capacity (T-AOC), and glutathione peroxidase (GSH-px) activity were significantly lower in obese subjects than in controls, while levels of serum malondialdehyde (MDA) were significantly higher. Circulating EPCs were positively associated with SOD (β = 0.306) and LnHOMA-IR (β = 0.223) and negatively associated with BMI (β = -0.321), serum visfatin (β = -0.236), and MDA (β = -0.293).
  
  
  CONCLUSIONS
  The quantity of circulating EPCs decreases in obese individuals, along with increased serum visfatin and oxidative stress product. Visfatin and oxidative stress might therefore impact on the circulating EPCs in obese populations.
authors.affiliation: Hebei Research Institute for Endocrine and Metabolic Diseases , Shijiazhuang, Hebei , P.R. China .
authors.forename: Shuchun
authors.initials: S
authors.lastname: Chen
authors.forename: Lina
authors.initials: L
authors.lastname: Sun
authors.forename: Haina
authors.initials: H
authors.lastname: Gao
authors.forename: Luping
authors.initials: L
authors.lastname: Ren
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Guangyao
authors.initials: G
authors.lastname: Song
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D015992:Body Mass Index; D016022:Case-Control Studies; D016207:Cytokines / Q000097:blood*; D066026:Endothelial Progenitor Cells / Q000473:pathology* / Q000502:physiology*; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D054409:Nicotinamide Phosphoribosyltransferase / Q000097:blood*; D009765:Obesity / Q000097:blood* / Q000473:pathology*; D018384:Oxidative Stress / Q000502:physiology*; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000975:Antioxidants; D016207:Cytokines; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human
keywords: Endothelial progenitor cells; obesity; oxidative stress; visfatin