title: Collagen type-I leads to in vivo matrix mineralization and secondary stabilization of Mg-Zr-Ca alloy implants.
abstract: Biodegradable magnesium-zirconia-calcium (Mg-Zr-Ca) alloy implants were coated with Collagen type-I (Coll-I) and assessed for their rate and efficacy of bone mineralization and implant stabilization. The phases, microstructure and mechanical properties of these alloys were analyzed using X-ray diffraction (XRD), optical microscopy and compression test, respectively, and the corrosion behavior was established by their hydrogen production rate in simulated body fluid (SBF). Coll-I extracted from rat tail, and characterized using fourier transform infrared (FT-IR) spectroscopy, was used for dip-coating the Mg-based alloys. The coated alloys were implanted into the femur bones of male New Zealand white rabbits. In vivo bone formation around the implants was quantified by measuring the bone mineral content/density (BMC/BMD) using dual-energy X-ray absorptiometry (DXA). Osseointegration of the implant and new bone mineralization was visualized by histological and immunohistochemical analysis. Upon surface coating with Coll-I, these alloys demonstrated high surface energy showing enhanced performance as an implant material that is suitable for rapid and efficient new bone tissue induction with optimal mineral content and cellular properties. The results demonstrate that Coll-I coated Mg-Zr-Ca alloys have a tendency to form superior trabecular bone structure with better osteoinduction around the implants and higher implant secondary stabilization, through the phenomenon of contact osteogenesis, compared to the control and uncoated ones in shorter periods of implantation. Hence, Coll-I surface coating of Mg-Zr-Ca alloys is a promising method for expediting new bone formation in vivo and enhancing osseointegration in load bearing implant applications.
authors.affiliation: Institute for Frontier Materials, Deakin University, Waurn Ponds, Geelong, Victoria 3217, Australia.
authors.forename: Dolly
authors.initials: D
authors.lastname: Mushahary
authors.affiliation: Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, Hawthorn, Victoria 3122, Australia.
authors.forename: Cuie
authors.initials: C
authors.lastname: Wen
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology, Hyderabad, Andhra Pradesh 500007, India.
authors.forename: Jerald Mahesh
authors.initials: JM
authors.lastname: Kumar
authors.affiliation: Advanced Material Research and Development Center, Zhejiang Industry & Trade Vocational College, Wenzhou, Zhejiang 325003, China.
authors.forename: Jixing
authors.initials: J
authors.lastname: Lin
authors.affiliation: National Institute of Nutrition (ICMR), Hyderabad, Andhra Pradesh 500007, India.
authors.forename: Nemani
authors.initials: N
authors.lastname: Harishankar
authors.affiliation: Institute for Frontier Materials, Deakin University, Waurn Ponds, Geelong, Victoria 3217, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Hodgson
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology, Hyderabad, Andhra Pradesh 500007, India.
authors.forename: Gopal
authors.initials: G
authors.lastname: Pande
authors.affiliation: Institute for Frontier Materials, Deakin University, Waurn Ponds, Geelong, Victoria 3217, Australia. Electronic address: yuncang.li@deakin.edu.au.
authors.forename: Yuncang
authors.initials: Y
authors.lastname: Li
mesh_terms: D016475:3T3 Cells; D015502:Absorptiometry, Photon; D000497:Alloys; D000818:Animals; D015519:Bone Density; D002113:Calcification, Physiologic; D002118:Calcium; D024042:Collagen Type I / Q000378:metabolism*; D008274:Magnesium; D051379:Mice; D016348:Osseointegration; D019736:Prostheses and Implants; D013499:Surface Properties; D014961:X-Ray Diffraction; D015032:Zinc
chemical_list: D000497:Alloys; D024042:Collagen Type I; D008274:Magnesium; D015032:Zinc; D002118:Calcium
keywords: DXA; Matrix mineralization; Osteoblasts; Osteoinduction; Peri-implant