title: Effects of pulsed electromagnetic field on differentiation of HUES-17 human embryonic stem cell line.
abstract: Electromagnetic fields are considered to potentially affect embryonic development, but the mechanism is still unknown. In this study, human embryonic stem cell (hESC) line HUES-17 was applied to explore the mechanism of exposure on embryonic development to pulsed electromagnetic field (PEMF) for 400 pulses at different electric field intensities and the differentiation of HUES-17 cells was observed after PEMF exposure. The expression of alkaline phosphatase (AP), stage-specific embryonic antigen-3 (SSEA-3), SSEA-4 and the mRNA level and protein level of Oct4, Sox2 and Nanog in HUES-17 cells remained unchanged after PEMF exposure at the electric field intensities of 50, 100, 200 or 400 kV/m. Four hundred pulses PEMF exposure at the electric field intensities of 50, 100, 200 or 400 kV/m did not affect the differentiation of HUES-17 cells. The reason why electromagnetic fields affect embryonic development may be due to other mechanisms rather than affecting the differentiation of embryonic stem cells.
authors.affiliation: Department of Radiation Medicine, the Fourth Military Medical University, Xi'an 710032, China. wuyilin710032@hotmail.com.
authors.forename: Yi-Lin
authors.initials: YL
authors.lastname: Wu
authors.affiliation: Department of Pathology and Pathophysiology, the Fourth Military Medical University, Xi'an 710032, China. shirongm@gmail.com.
authors.forename: Shi-Rong
authors.initials: SR
authors.lastname: Ma
authors.affiliation: Department of Neurosurgery, the First Hospital of Jilin University, Changchun 130021, China. flwrdng@gmail.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.affiliation: Department of Pharmaceutics, the Fourth Military Medical University, Xi'an 710032, China. tengzenghui710032@hotmail.com.
authors.forename: Zeng-Hui
authors.initials: ZH
authors.lastname: Teng
authors.affiliation: Experiment Teaching Center, the Fourth Military Medical University, Xi'an 710032, China. liangxiangyan710032@hotmail.com.
authors.forename: Xiang-Yan
authors.initials: XY
authors.lastname: Liang
authors.affiliation: Department of Radiation Medicine, the Fourth Military Medical University, Xi'an 710032, China. kangchu@fmmu.edu.cn.
authors.forename: Guo-Zhen
authors.initials: GZ
authors.lastname: Guo
authors.affiliation: Experiment Teaching Center, the Fourth Military Medical University, Xi'an 710032, China. hfzhang@fmmu.edu.cn.
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Zhang
authors.affiliation: Department of Radiation Medicine, the Fourth Military Medical University, Xi'an 710032, China. likangchu@hotmail.com.
authors.forename: Kang-Chu
authors.initials: KC
authors.lastname: Li
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D004574:Electromagnetic Fields; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D055746:Stage-Specific Embryonic Antigens / Q000378:metabolism
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D055746:Stage-Specific Embryonic Antigens; C052924:stage-specific embryonic antigen-3; C066584:stage-specific embryonic antigen-4; D000469:Alkaline Phosphatase