title: Use of magnetic polyaniline/maghemite nanocomposite for DNA retrieval from aqueous solutions.
abstract: We demonstrated that the magnetic polyaniline/maghemite nanocomposite (Pani/γ-Fe2O3 MNC) is an efficient agent for retrieval of pure double stranded deoxyribonucleic acid (dsDNA) chains from aqueous solutions. The dsDNA chains used in the retrieval experiments were of sodium salt of Salmon Sperm DNA. Based on λ=260 nm absorption measurements, we have employed UV-Vis spectroscopy to estimate the concentration of DNA present in solutions, before and after the interaction with the MNC. The best results corresponded to a maximum amount of 75.2 mg of DNA absorbed per gram of MNC reached within only 10 min of joint exposure into the aqueous solution. After magnetic separation of the fully DNA-loaded Pani/γ-Fe2O3 MNC, we achieved essentially complete DNA desorption by appropriate changes in the pH of the solution. We have shown that it is possible to recycle the use of these MNC in several adsorption-desorption cycles. By comparing the present results to those of other DNA retrieval systems reported in the literature, we argued that the Pani/γ-Fe2O3 MNC here described represent a promising low-cost material for use as a fast, simple and efficient method of DNA separation and concentration.
authors.affiliation: Pós-Graduação em Ciência de Materiais, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Medina-Llamas
authors.affiliation: Pós-Graduação em Ciência de Materiais, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil.
authors.forename: Alicia Elizabeth
authors.initials: AE
authors.lastname: Chávez-Guajardo
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil.
authors.forename: Cesar Augusto Souza
authors.initials: CA
authors.lastname: Andrade
authors.affiliation: Departamento de Engenharia Mecânica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil.
authors.forename: Kleber Gonçalves Bezerra
authors.initials: KG
authors.lastname: Alves
authors.affiliation: Pós-Graduação em Ciência de Materiais, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil; Departamento de Física, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil. Electronic address: celso@df.ufpe.br.
authors.forename: Celso Pinto
authors.initials: CP
authors.lastname: de Melo
mesh_terms: D000327:Adsorption; D000814:Aniline Compounds / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods; D004247:DNA / Q000302:isolation & purification*; D005290:Ferric Compounds / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000814:Aniline Compounds; D005290:Ferric Compounds; C416807:polyaniline; C000499:ferric oxide; D004247:DNA
keywords: DNA retrieval; Pani/γ-Fe(2)O(3) magnetic nanocomposite; UV–Vis spectroscopy