title: Molecular determinants of the recognition of ulipristal acetate by oxo-steroid receptors.
abstract: The human progesterone receptor (PR) plays a key role in reproductive function in women. PR antagonists have numerous applications in female health care including regular and emergency contraception, and treatment of hormone-related pathological conditions such as breast cancer, endometriosis, and leiomyoma. The main factor limiting their long-term administration is the fact that they cross-bind to other oxo-steroid receptors. Ulipristal acetate (UPA), a highly potent PR antagonist, has recently come onto the market and is much more selective for PR than the other oxo-steroid receptors (androgen, AR, glucocorticoid, GR, and mineralocorticoid, MR receptors) and, remarkably, it displays lower GR-inactivating potency than RU486. We adopted a structural approach to characterizing the binding of UPA to the oxo-steroid receptors at the molecular level. We solved the X-ray crystal structure of the ligand-binding domain (LBD) of the human PR complexed with UPA and a peptide from the transcriptional corepressor SMRT. We used the X-ray crystal structure of the GR in its antagonist conformation to dock UPA within its ligand-binding cavity. Finally, we generated three-dimensional models of the LBD of androgen and mineralocorticoid receptors (AR and MR) in an antagonist conformation and docked UPA within them. Comparing the structures revealed that the network of stabilizing contacts between the UPA C11 aryl group and the LBD is responsible for its high PR antagonist potency. It also showed that it is the inability of UPA to contact Gln642 in GR that explains why it has lower potency in inactivating GR than RU486. Finally, we found that the binding pockets of AR and MR are too small to accommodate UPA, and allowed us to propose that the extremely low sensitivity of MR to UPA is due to inappropriate interactions with the C11 substituent. All these findings open new avenues for designing new PR antagonist compounds displaying greater selectivity.
authors.affiliation: Inserm U773, Centre de Recherche Biomédicale Bichat-Beaujon CRB3, Paris, France; Université Denis Diderot, Site Bichat, Paris, France.
authors.forename: I
authors.initials: I
authors.lastname: Petit-Topin
authors.affiliation: Inserm U773, Centre de Recherche Biomédicale Bichat-Beaujon CRB3, Paris, France; Université Denis Diderot, Site Bichat, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Fay
authors.affiliation: Laboratoire HRA Pharma, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Resche-Rigon
authors.affiliation: Laboratoire HRA Pharma, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Ulmann
authors.affiliation: Laboratoire HRA Pharma, Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: Gainer
authors.affiliation: Inserm U773, Centre de Recherche Biomédicale Bichat-Beaujon CRB3, Paris, France; Université Denis Diderot, Site Bichat, Paris, France.
authors.forename: M-E
authors.initials: ME
authors.lastname: Rafestin-Oblin
authors.affiliation: Inserm U773, Centre de Recherche Biomédicale Bichat-Beaujon CRB3, Paris, France; Université Denis Diderot, Site Bichat, Paris, France; Inserm U693, Le Kremlin - Bicêtre F94276, France; Faculté de Médecine Paris-Sud, Univ Paris-Sud, UMR-S693, Le Kremlin - Bicêtre F94276, France. Electronic address: jerome.fagart@inserm.fr.
authors.forename: J
authors.initials: J
authors.lastname: Fagart
mesh_terms: D001665:Binding Sites; D018360:Crystallography, X-Ray; D057809:HEK293 Cells; D006727:Hormone Antagonists / Q000494:pharmacology*; D006801:Humans; D008958:Models, Molecular; D009649:Norpregnadienes / Q000494:pharmacology*; D011487:Protein Conformation; D011944:Receptors, Androgen / Q000737:chemistry / Q000378:metabolism*; D018161:Receptors, Mineralocorticoid / Q000737:chemistry / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000819:agonists / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D006727:Hormone Antagonists; D009649:Norpregnadienes; D011944:Receptors, Androgen; D018161:Receptors, Mineralocorticoid; D011980:Receptors, Progesterone; C555622:ulipristal acetate
keywords: Antagonist; Crystal; Nuclear receptor; Progesterone receptor; Reproduction; Selectivity; Steroids; structure