title: Preparation of crude rough microsomes from tissue culture cells.
abstract: There are various procedures for isolating microsomal fractions from tissue culture cells. The essential conditions for each step of one procedure are described here. Notes for special circumstances are included so that the procedure can be modified according to the experimental purpose.
authors.affiliation: Department of Cell Biology, NYU Medical School, New York, New York 10016.
authors.forename: David D
authors.initials: DD
authors.lastname: Sabatini
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002458:Cell Fractionation; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D003513:Cycloheximide / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D008861:Microsomes / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology
chemical_list: D011500:Protein Synthesis Inhibitors; D003513:Cycloheximide