title: Low molecular weight heparin improves proteinuria in rats with L-NAME induced preeclampsia by decreasing the expression of nephrin, but not podocin.
abstract: OBJECTIVE
  We investigated the relationship between proteinuria in L-NAME induced preeclampsia and the expression of nephrin and podocin, and the effect of low-molecular-weight-heparin (LMWH) on proteinuria in rats.
  
  
  METHODS
  We detected nephrin and podocin expression of kidneys of pregnant rats after L-NAME and after LMWH intervening pregnant rats.
  
  
  RESULTS
  Glomerular nephrin expression in L-NAME induced preeclampsia significantly decreased, but not podocin. Nephrin was relatively increased after LMWH intervention and this was accompanied by a decrease in proteinuria.
  
  
  CONCLUSIONS
  We demonstrate that down-regulation of nephrin is involved in L-NAME induced proteinuria, and that LMWH reduces proteinuria by up-regulation of neprhin.
authors.affiliation: Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan , P.R. China.
authors.forename: Wanlu
authors.initials: W
authors.lastname: Liu
authors.forename: Fuyuan
authors.initials: F
authors.lastname: Qiao
authors.forename: Haiyi
authors.initials: H
authors.lastname: Liu
authors.forename: Xun
authors.initials: X
authors.lastname: Gong
authors.forename: Xinwei
authors.initials: X
authors.lastname: Shi
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Li
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D005260:Female; D006495:Heparin, Low-Molecular-Weight / Q000494:pharmacology / Q000627:therapeutic use*; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester; D011225:Pre-Eclampsia / Q000188:drug therapy*; D011247:Pregnancy; D011507:Proteinuria / Q000188:drug therapy*; D011897:Random Allocation; D017208:Rats, Wistar
chemical_list: D006495:Heparin, Low-Molecular-Weight; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C407223:NPHS2 protein; C113267:nephrin; D019331:NG-Nitroarginine Methyl Ester
keywords: Animal model; low-molecular-weight-heparin; nephrin; podocin; preeclampsia