title: Microwave-assisted synthesis of surface-enhanced Raman scattering nanoprobes for cellular sensing.
abstract: The fabrication of 4-mercaptobenzoic acid (4-MBA) antibody-functionalized gold nanoparticles via microwave technology for surface-enhanced Raman scattering (SERS)-based cellular nanosensing is reported. Nanoprobes were characterized by UV-vis absorbance, Raman scattering properties, and observed by TEM imaging. Results showed that microwave irradiation rapidly yielded nanoprobes with significant Raman scattering intensity and suitable stability to support antibody conjugation in under 10min. Functionalized nanoprobes demonstrated the ability to map the expression of vascular adhesion molecule-1 (VCAM-1) in human coronary artery endothelial (HCAE) cells, indicating that microwave fabrication presents a viable and rapid approach to SERS nanoprobe construction. The successful application of SERS nanoprobes to localize biomarker expression in vitro may ultimately be used for early diagnostic and preventative functions in medicine.
authors.affiliation: Department of Biomedical Engineering, McGill University, 3773 University, Montréal, QC, Canada H3A 2B6.
authors.forename: Kristen
authors.initials: K
authors.lastname: Bowey
authors.affiliation: Montréal Heart Institute, Université de Montréal, 5000 Bélanger, Montréal, QC, Canada H1T 1C8.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Tanguay
authors.affiliation: Department of Nanoscience, University of North Carolina at Greensboro, 2907 East Lee Street, Greensboro, NC 27401, USA. Electronic address: m_sandro@uncg.edu.
authors.forename: Marinella G
authors.initials: MG
authors.lastname: Sandros
authors.affiliation: Department of Biomedical Engineering, McGill University, 3773 University, Montréal, QC, Canada H3A 2B6; Faculty of Dentistry, McGill University, 3640 University, Montréal, QC, Canada H3A 0C7. Electronic address: maryam.tabrizian@mcgill.ca.
authors.forename: Maryam
authors.initials: M
authors.lastname: Tabrizian
mesh_terms: D015374:Biosensing Techniques; D002460:Cell Line; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008872:Microwaves; D049329:Nanostructures; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013499:Surface Properties; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis
chemical_list: D019010:Vascular Cell Adhesion Molecule-1
keywords: Biomarker detection; Cardiovascular disease; Human coronary artery endothelial cells; Plasmonic nanoparticles; Surface-enhanced Raman scattering