title: Modulation of nitrosative stress via glutathione-dependent formaldehyde dehydrogenase and S-nitrosoglutathione reductase.
abstract: Glutathione-dependent formaldehyde dehydrogenase (GFD) from Taiwanofungus camphorata plays important roles in formaldehyde detoxification and antioxidation. The enzyme is bifunctional. In addition to the GFD activity, it also functions as an effective S-nitrosoglutathione reductase (GSNOR) against nitrosative stress. We investigated the modulation of HEK (human embryonic kidney) 293T cells under nitrosative stress by transfecting a codon optimized GFD cDNA from Taiwanofungus camphorata (Tc-GFD-O) to these cells. The parental and transfected HEK 293T cells were then subjected to S-nitrosoglutathione treatment to induce nitrosative stress. The results showed that in Tc-GFD-O-transfected 293T cells, the expression and activity of GFD increased. Additionally, these cells under the nitrosative stress induced by S-nitrosoglutathione showed both higher viability and less apoptosis than the parental 293T cells. This finding suggests that the Tc-GFD-O in HEK 293T cells may provide a protective function under nitrosative stress.
authors.affiliation: Institute of Biotechnology, National Changhua University of Education, Changhua 500, Taiwan. kencf@cc.ncue.edu.tw.
authors.forename: Chuian-Fu
authors.initials: CF
authors.lastname: Ken
authors.affiliation: Department of Bioscience and Biotechnology and Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung 202, Taiwan. huang_sara1208@yahoo.com.tw.
authors.forename: Chih-Yu
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Department of Chemistry, Western Illinois University, Macomb, IL 61455, USA. L-Wen@wiu.edu.
authors.forename: Lisa
authors.initials: L
authors.lastname: Wen
authors.affiliation: Department of Chemistry, Western Illinois University, Macomb, IL 61455, USA. J-Huang3@wiu.edu.
authors.forename: Jenq-Kuen
authors.initials: JK
authors.lastname: Huang
authors.affiliation: Department of Bioscience and Biotechnology and Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung 202, Taiwan. B0220@mail.ntou.edu.tw.
authors.forename: Chi-Tsai
authors.initials: CT
authors.lastname: Lin
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D026422:S-Nitrosoglutathione / Q000494:pharmacology
chemical_list: D026422:S-Nitrosoglutathione; D000445:Aldehyde Oxidoreductases; C087006:formaldehyde dehydrogenase, glutathione-independent