title: Characterization of Acetobacter pomorum KJY8 isolated from Korean traditional vinegar.
abstract: Acetobacter sp. strains were isolated from traditional vinegar collected in Daegu city and Gyeongbuk province. The strain KJY8 showing a high acetic acid productivity was isolated and characterized by phenotypic, chemotaxonomic, and phylogenetic inference based on 16S rRNA sequence analysis. The chemotaxonomic and phylogenetic analyses revealed the isolate to be a strain of Acetobacter pomorum. The isolate showed a G+C content of 60.8 mol%. It contained LL-diaminopimelic acid (LL-A2pm) as the cell wall amino acid and ubiquinone Q9 (H6) as the major quinone. The predominant cellular fatty acids were C18:1w9c, w12t, and w7c. Strain KJY8 grew rapidly on glucose-yeast extract (GYC) agar and formed pale white colonies with smooth to rough surfaces. The optimum cultivation conditions for acetic acid production by the KJY8 strain were 20°C and pH 3.0, with an initial ethanol concentration of 9% (w/v) to produce an acetic acid concentration of 8% (w/v).
authors.affiliation: Fermented Food Science Division, Department of Agrofood Resources, NAAS, RDA, Jeollabuk-do 565-850, Republic of Korea.
authors.forename: Chang Ho
authors.initials: CH
authors.lastname: Baek
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Park
authors.forename: Seong Yeol
authors.initials: SY
authors.lastname: Baek
authors.forename: Seok-Tae
authors.initials: ST
authors.lastname: Jeong
authors.forename: Myoung-Dong
authors.initials: MD
authors.lastname: Kim
authors.forename: Joong-Ho
authors.initials: JH
authors.lastname: Kwon
authors.forename: Yong-Jin
authors.initials: YJ
authors.lastname: Jeong
authors.forename: Soo-Hwan
authors.initials: SH
authors.lastname: Yeo
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D000091:Acetobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005516:Food Microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid; D019342:Acetic Acid