title: Naringin prevents ovariectomy-induced osteoporosis and promotes osteoclasts apoptosis through the mitochondria-mediated apoptosis pathway.
abstract: Naringin, the primary active compound of the traditional Chinese medicine Rhizoma drynariae, possesses many pharmacological activities. The present study is an effort to explore the anti-osteoporosis potential of naringin in vivo and in vitro. In vivo, we used ovariectomized rats to clarify the mechanisms by which naringin anti-osteoporosis. In vitro, we used osteoclasts to investigate naringin promotes osteoclasts apoptosis. Naringin was effective at enhancing BMD, trabecular thickness, bone mineralization, and mechanical strength in a dose-dependent manner. The result of RT-PCR analysis revealed that naringin down-regulated the mRNA expression levels of BCL-2 and up-regulated BAX, caspase-3 and cytochrome C. In addition, naringin significantly reduced the bone resorption area in vitro. These findings suggest that naringin promotes the apoptosis of osteoclasts by regulating the activity of the mitochondrial apoptosis pathway and prevents OVX-induced osteoporosis in rats.
authors.affiliation: Tianjin Institute of Orthopedics in Traditional Chinese and Western Medicine, No. 155, Munan Road, Tianjin TJ 300050, China; Graduate School of Tianjin Medical University, No. 22, Qixiangtai Street, Heping District, Tianjin 300070, China.
authors.forename: Fengbo
authors.initials: F
authors.lastname: Li
authors.affiliation: Tianjin Institute of Orthopedics in Traditional Chinese and Western Medicine, No. 155, Munan Road, Tianjin TJ 300050, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Sun
authors.affiliation: Tianjin Institute of Orthopedics in Traditional Chinese and Western Medicine, No. 155, Munan Road, Tianjin TJ 300050, China.
authors.forename: Jianxiong
authors.initials: J
authors.lastname: Ma
authors.affiliation: Tianjin Institute of Orthopedics in Traditional Chinese and Western Medicine, No. 155, Munan Road, Tianjin TJ 300050, China. Electronic address: gengxiao502@163.com.
authors.forename: Xinlong
authors.initials: X
authors.lastname: Ma
authors.affiliation: Tianjin Institute of Orthopedics in Traditional Chinese and Western Medicine, No. 155, Munan Road, Tianjin TJ 300050, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Tianjin Institute of Orthopedics in Traditional Chinese and Western Medicine, No. 155, Munan Road, Tianjin TJ 300050, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Tianjin Institute of Orthopedics in Traditional Chinese and Western Medicine, No. 155, Munan Road, Tianjin TJ 300050, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Tian
authors.affiliation: Graduate School of Tianjin Medical University, No. 22, Qixiangtai Street, Heping District, Tianjin 300070, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Tianjin Institute of Orthopedics in Traditional Chinese and Western Medicine, No. 155, Munan Road, Tianjin TJ 300050, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Han
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015519:Bone Density / Q000187:drug effects; D050071:Bone Density Conservation Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001862:Bone Resorption / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D002113:Calcification, Physiologic / Q000187:drug effects; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D045304:Cytochromes c / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D044950:Flavanones / Q000302:isolation & purification / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010010:Osteoclasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010024:Osteoporosis / Q000209:etiology / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D010052:Ovariectomy / Q000009:adverse effects*; D010936:Plant Extracts / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D050071:Bone Density Conservation Agents; D044950:Flavanones; D010936:Plant Extracts; D019253:Proto-Oncogene Proteins c-bcl-2; D045304:Cytochromes c; D053148:Caspase 3; C005274:naringin
keywords: Apoptosis; Naringin; Osteoclast; Osteoporosis