title: Role of α-adrenergic vasoconstriction in regulating skeletal muscle blood flow and vascular conductance during forearm exercise in ageing humans.
abstract: In healthy humans, ageing is typically associated with reduced skeletal muscle blood flow and vascular conductance during exercise. Further, there is a marked increase in resting sympathetic nervous system (SNS) activity with age, yet whether augmented SNS-mediated α-adrenergic vasoconstriction contributes to the age-associated impairment in exercising muscle blood flow and vascular tone in humans is unknown. We tested the hypothesis that SNS-mediated vasoconstriction is greater in older than young adults and limits muscle (forearm) blood flow (FBF) during graded handgrip exercise (5, 15, 25% maximal voluntary contraction (MVC)). FBF was measured (Doppler ultrasound) and forearm vascular conductance (FVC) was calculated in 11 young (21 ± 1 years) and 12 older (62 ± 2 years) adults in control conditions and during combined local α- and β-adrenoreceptor blockade via intra-arterial infusions of phentolamine and propranolol, respectively. Under control conditions, older adults exhibited significantly lower FBF and FVC at 15% MVC exercise (22.6 ± 1.3 vs. 29 ± 3.3 ml min(-1) 100 g forearm fat-free mass (FFM)(-1) and 21.7 ± 1.2 vs. 33.6 ± 4.0 ml min(-1) 100 g FFM(-1) 100 mmHg(-1); P < 0.05) and 25% MVC exercise (37.4 ± 1.4 vs. 46.0 ± 4.9 ml min(-1) 100 g FFM(-1) and 33.7 ± 1.4 vs. 49.0 ± 5.7 ml min(-1) 100 g FFM(-1) 100 mmHg(-1); P < 0.05), whereas there was no age group difference at 5% MVC exercise. Local adrenoreceptor blockade increased FBF and FVC at rest and during exercise in both groups, although the increase in FBF and FVC from rest to steady-state exercise was similar in young and older adults across exercise intensities, and thus the age-associated impairment in FBF and FVC persisted. Our data indicate that during graded intensity handgrip exercise, the reduced FVC and subsequently lower skeletal muscle blood flow in older healthy adults is not due to augmented sympathetic vasoconstriction, but rather due to impairments in local signalling or structural limitations in the peripheral vasculature with advancing age.
authors.affiliation: Human Cardiovascular Physiology Laboratory, Department of Health and Exercise Science, Colorado State University, Fort Collins, CO, 80523-1582, USA.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Richards
authors.affiliation: Medical Center of the Rockies Foundation, Poudre Valley Health System, Loveland, CO, 80538, USA.
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Luckasen
authors.affiliation: Medical Center of the Rockies Foundation, Poudre Valley Health System, Loveland, CO, 80538, USA.
authors.forename: Dennis G
authors.initials: DG
authors.lastname: Larson
authors.affiliation: Human Cardiovascular Physiology Laboratory, Department of Health and Exercise Science, Colorado State University, Fort Collins, CO, 80523-1582, USA Vascular Physiology Research Group, Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523-1582, USA frank.dinenno@colostate.edu.
authors.forename: Frank A
authors.initials: FA
authors.lastname: Dinenno
mesh_terms: D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology*; D000375:Aging; D005260:Female; D005542:Forearm; D018737:Hand Strength / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000187:drug effects; D010646:Phentolamine / Q000494:pharmacology*; D012039:Regional Blood Flow; D014655:Vascular Resistance / Q000187:drug effects / Q000502:physiology; D014661:Vasoconstriction / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects; D055815:Young Adult
chemical_list: D000317:Adrenergic alpha-Antagonists; D010646:Phentolamine