title: Trophic Effects and Regenerative Potential of Mobilized Mesenchymal Stem Cells From Bone Marrow and Adipose Tissue as Alternative Cell Sources for Pulp/Dentin Regeneration.
abstract: Dental pulp stem cell (DPSC) subsets mobilized by granulocyte-colony-stimulating factor (G-CSF) are safe and efficacious for complete pulp regeneration. The supply of autologous pulp tissue, however, is very limited in the aged. Therefore, alternative sources of mesenchymal stem/progenitor cells (MSCs) are needed for the cell therapy. In this study, DPSCs, bone marrow (BM), and adipose tissue (AD)-derived stem cells of the same individual dog were isolated using G-CSF-induced mobilization (MDPSCs, MBMSCs, and MADSCs). The positive rates of CXCR4 and G-CSFR in MDPSCs were similar to MADSCs and were significantly higher than those in MBMSCs. Trophic effects of MDPSCs on angiogenesis, neurite extension, migration, and antiapoptosis were higher than those of MBMSCs and MADSCs. Pulp-like loose connective tissues were regenerated in all three MSC transplantations. Significantly higher volume of regenerated pulp and higher density of vascularization and innervation were observed in response to MDPSCs compared to MBMSC and MADSC transplantation. Collagenous matrix containing dentin sialophosphoprotein (DSPP)-positive odontoblast-like cells was the highest in MBMSCs and significantly higher in MADSCs compared to MDPSCs. MBMSCs and MADSCs, therefore, have potential for pulp regeneration, although the volume of regenerated pulp tissue, angiogenesis, and reinnervation, were less. Thus, in conclusion, an alternative cell source for dental pulp/dentin regeneration are stem cells from BM and AD tissue.
authors.affiliation: Department of Dental Regenerative Medicine, Center of Advanced Medicine for Dental and Oral Diseases, National Center for Geriatrics and Gerontology, Research Institute, Obu, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Murakami
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Koichiro
authors.initials: K
authors.lastname: Iohara
authors.forename: Yohei
authors.initials: Y
authors.lastname: Osako
authors.forename: Yujiro
authors.initials: Y
authors.lastname: Hirose
authors.forename: Misako
authors.initials: M
authors.lastname: Nakashima
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D003782:Dental Pulp / Q000166:cytology / Q000502:physiology*; D004285:Dogs; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D023421:Models, Animal; D009804:Odontoblasts / Q000166:cytology / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000378:metabolism; D012038:Regeneration; D012795:Sialoglycoproteins / Q000378:metabolism; D014182:Transplantation, Autologous; D000099311:Dentin Sialophosphoprotein
chemical_list: D017077:Culture Media, Conditioned; D016326:Extracellular Matrix Proteins; D016179:Granulocyte Colony-Stimulating Factor; D010750:Phosphoproteins; D019718:Receptors, CXCR4; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein