title: Development and validation of a specific and sensitive gas chromatography tandem mass spectrometry method for the determination of bisphenol A residues in a large set of food items.
abstract: BPA-containing products are widely used in foodstuffs packaging as authorized within the European Union (UE no. 10/2011). Therefore, foods and beverages are in contact with BPA which can migrate from food contact material to foodstuffs. An accurate assessment of the exposure of the consumers to BPA is crucial for a non-ambiguous risk characterization. In this context, an efficient analytical method using gas chromatography coupled to tandem mass spectrometry (GC-MS/MS), in the selected reaction monitoring (SRM) mode, was developed for the quantification of BPA in foodstuffs at very low levels (<0.5μgkg(-1)). A standard operating procedure, based on the combination of two successive solid phase extractions (SPE), was developed for various liquid and solid foodstuffs. The use of (13)C12-BPA as internal standard allowed accurate quantification of BPA by isotopic dilution. Control charts based on both blank and certified materials have been implemented to ensure analytical data quality. The developed analytical method has been validated according to in-house validation requirements. R(2) was better than 0.9990 within the range [0-100μgkg(-1)], the trueness was 4.2%. Repeatability and within-laboratory reproducibility ranged from 7.5% to 19.0% and 2.5% to 12.2%, respectively, at 0.5 and 5.0μgkg(-1) depending on the matrices tested for. The detection and quantification limits were 0.03 and 0.10μgkg(-1), respectively. The reporting limit was 0.35μgkg(-1), taking into account the mean of the laboratory background contamination. The global uncertainty was 22.2% at 95% confidence interval.
authors.affiliation: LUNAM Université, Oniris, Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), Nantes F-44307, France. Electronic address: laberca@oniris-nantes.fr.
authors.forename: Y
authors.initials: Y
authors.lastname: Deceuninck
authors.affiliation: LUNAM Université, Oniris, Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), Nantes F-44307, France.
authors.forename: E
authors.initials: E
authors.lastname: Bichon
authors.affiliation: LUNAM Université, Oniris, Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), Nantes F-44307, France.
authors.forename: S
authors.initials: S
authors.lastname: Durand
authors.affiliation: French Agency for Food, Environmental and Occupational Health & Safety (ANSES, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail, Direction de l'Evaluation des Risques, 27-31 Avenue du Général Leclerc, 94701 Maisons-Alfort, France.
authors.forename: N
authors.initials: N
authors.lastname: Bemrah
authors.affiliation: LUNAM Université, Oniris, Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), Nantes F-44307, France.
authors.forename: Z
authors.initials: Z
authors.lastname: Zendong
authors.affiliation: LUNAM Université, Oniris, Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), Nantes F-44307, France; INRA, Nantes F-44307, France.
authors.forename: M L
authors.initials: ML
authors.lastname: Morvan
authors.affiliation: LUNAM Université, Oniris, Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), Nantes F-44307, France.
authors.forename: P
authors.initials: P
authors.lastname: Marchand
authors.affiliation: LUNAM Université, Oniris, Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), Nantes F-44307, France.
authors.forename: G
authors.initials: G
authors.lastname: Dervilly-Pinel
authors.affiliation: LUNAM Université, Oniris, Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), Nantes F-44307, France; INRA, Nantes F-44307, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Antignac
authors.affiliation: French Agency for Food, Environmental and Occupational Health & Safety (ANSES, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail, Direction de l'Evaluation des Risques, 27-31 Avenue du Général Leclerc, 94701 Maisons-Alfort, France.
authors.forename: J C
authors.initials: JC
authors.lastname: Leblanc
authors.affiliation: LUNAM Université, Oniris, Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), Nantes F-44307, France.
authors.forename: B
authors.initials: B
authors.lastname: Le Bizec
mesh_terms: D001559:Benzhydryl Compounds / Q000032:analysis*; D002138:Calibration; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D057230:Limit of Detection; D010636:Phenols / Q000032:analysis*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Foodstuffs; GC–MS/MS; Isotopic dilution; MIP; Validation