title: Effects of oxidation on redox and cytotoxic properties of copper complex of Aβ1-16 peptide.
abstract: The effect of oxidation on redox and cytotoxic properties of copper complex of amyloid beta (Aβ) peptide was studied by gamma radiolysis. The oxidation of Aβ1-16 and Aβ1-16/Cu(II) complex was carried out using hydroxyl ((•)OH) radicals produced by gamma radiolysis and the products were analyzed using mass spectrometry. The presence of Cu(II) was found to enhance the oxidation of Aβ1-16 peptide. The oxidation of residues Asp1, His6, and His13 was enhanced due to their involvement in copper binding. The oxidation of His residues of Aβ1-16 peptide, which are chiefly responsible for copper binding, resulted in altered redox properties and subsequently in higher cytotoxicity of the Aβ1-16 peptide in SH-SY5Y cells.
authors.affiliation: Biometry and Nutrition Group, Agharkar Research Institute , Pune , India.
authors.forename: S N
authors.initials: SN
authors.lastname: Ramteke
authors.forename: G R
authors.initials: GR
authors.lastname: Walke
authors.forename: B N
authors.initials: BN
authors.lastname: Joshi
authors.forename: S
authors.initials: S
authors.lastname: Rapole
authors.forename: P P
authors.initials: PP
authors.lastname: Kulkarni
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000187:drug effects / Q000633:toxicity*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D017665:Hydroxyl Radical / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects; D010084:Oxidation-Reduction / Q000187:drug effects; D010446:Peptide Fragments / Q000737:chemistry* / Q000187:drug effects / Q000633:toxicity*; D013329:Structure-Activity Relationship
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C433241:amyloid beta-protein (1-16); D017665:Hydroxyl Radical; D003300:Copper
keywords: amyloid β; copper; free radicals; gamma radiolysis; mass spectrometry