title: rAAV/ABAD-DP-6His attenuates oxidative stress-induced injury of PC12 cells.
abstract: Our previous studies have revealed that amyloid β (Aβ)-binding alcohol dehydrogenase (ABAD) decoy peptide antagonizes Aβ42-induced neurotoxicity. However, whether it improves oxidative stress injury remains unclear. In this study, a recombinant adenovirus constitutively secreting and expressing Aβ-ABAD decoy peptide (rAAV/ABAD-DP-6His) was successfully constructed. Our results showed that rAAV/ABAD-DP-6His increased superoxide dismutase activity in hydrogen peroxide-induced oxidative stress-mediated injury of PC12 cells. Moreover, rAAV/ABAD-DP-6His decreased malondialdehyde content, intracellular Ca(2+) concentration, and the level of reactive oxygen species. rAAV/ABAD-DP-6His maintained the stability of the mitochondrial membrane potential. In addition, the ATP level remained constant, and apoptosis was reduced. Overall, the results indicate that rAAV/ABAD-DP-6His generates the fusion peptide, Aβ-ABAD decoy peptide, which effectively protects PC12 cells from oxidative stress injury induced by hydrogen peroxide, thus exerting neuroprotective effects.
authors.affiliation: Department of Neurology, the First Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Mingyue
authors.initials: M
authors.lastname: Jia
authors.affiliation: Department of Neurology, People's Hospital of Jilin Province, Changchun, Jilin Province, China.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Neurology, the First Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Radioactive Medicine Specialty, College of Public Health in Jilin University, Changchun, Jilin Province, China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Burn and Plastic Surgery, the General Hospital of CNPC in Jilin, Jilin, Jilin Province, China.
authors.forename: Dujuan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Neurology, the First Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Neurology, the First Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of Neurology, the First Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Neurology, the First Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
keywords: Alzheimer's disease; NSFC grant; adeno-associated virus; amyloid beta binding alcohol dehydrogenase; amyloid beta peptide; gene therapy; hydrogen peroxide; mitochondrial dysfunction; nerve regeneration; neural regeneration; neurodegenerative disease; oxidative stress