title: Transferrin receptor and ferritin-H are developmentally regulated in oligodendrocyte lineage cells.
abstract: Iron is an essential trophic element that is required for cell viability and differentiation, especially in oligodendrocytes, which consume relatively high rates of energy to produce myelin. Multiple iron metabolism proteins are expressed in the brain including transferrin receptor and ferritin-H. However, it is still unknown whether they are developmentally regulated in oligodendrocyte lineage cells for myelination. Here, using an in vitro cultured differentiation model of oligodendrocytes, we found that both transferrin receptor and ferritin-H are significantly upregulated during oligodendrocyte maturation, implying the essential role of iron in the development of oligodendrocytes. Additional different doses of Fe(3+) in the cultured medium did not affect oligodendrocyte precursor cell maturation or ferritin-H expression but decreased the expression of the transferrin receptor. These results indicate that upregulation of both transferrin receptor and ferritin-H contributes to maturation and myelination of oligodendrocyte precursor cells.
authors.affiliation: Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Guan
authors.affiliation: Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Han
authors.affiliation: Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
authors.forename: Wuchao
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Nie
keywords: development; ferritin-H; grants-supported paper; induced differentiation; iron; myelinization; neural regeneration; neurogenesis; neuroregeneration; oligodendrocyte; photographs-containing paper; proliferation; transferrin receptor