title: Antioxidant and antiproliferative activity of Stachys glutinosa L. ethanol extract.
abstract: Ethanol extracts of Stachys glutinosa L. (Lamiaceae) were investigated for antioxidative properties, as well as antiproliferative action on various cell lines. The antioxidant activities were investigated by ABTS (2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonic acid) assay, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, β-carotene/linoleic acid assay, scavenging of hydrogen peroxide (horseradish peroxidase test), superoxide anion scavenging, and hypochlorous acid scavenging (taurine test). The antioxidant activity was reported as IC50 and reveals antioxidant effects. Antiproliferative effects were measured in vitro on three cell lines: HepG2 (human hepatocarcinoma), MCF7 (breast human adenocarcinoma) and C2C12 (mouse myoblast) cell lines by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The ethanol extract induced variations in cell viability on all cell lines tested. At 200 μg/mL, the effects on cell viability were - 23%, - 27% and - 37%, respectively, for C2C12, MCF7 and HepG2.
authors.affiliation: a Department of Pharmacy , University G. d'Annunzio , Chieti , Italy.
authors.forename: Lidia
authors.initials: L
authors.lastname: Leporini
authors.forename: Luigi
authors.initials: L
authors.lastname: Menghini
authors.forename: Marzia
authors.initials: M
authors.lastname: Foddai
authors.forename: Giacomo Luigi
authors.initials: GL
authors.lastname: Petretto
authors.forename: Mario
authors.initials: M
authors.lastname: Chessa
authors.forename: Bruno
authors.initials: B
authors.lastname: Tirillini
authors.forename: Giorgio
authors.initials: G
authors.lastname: Pintore
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol; D006027:Glycosides / Q000737:chemistry; D006801:Humans; D020128:Inhibitory Concentration 50; D013058:Mass Spectrometry; D051379:Mice; D010636:Phenols / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D031366:Stachys / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D006027:Glycosides; D010636:Phenols; D010936:Plant Extracts; D000431:Ethanol
keywords: Stachys glutinosa L; antioxidant; antiproliferative activity; ethanol extracts