title: Hydrophilic C terminus of Salicornia europaea vacuolar Na(+)/H(+) antiporter is necessary for its function.
abstract: Plant vacuolar Na(+)/H(+) antiporters play important roles in cellular ion homeostasis,vacuolar pH regulation and sequestration of Na(+) ions into the vacuole. Previous research showed that hydrophilic C-terminal region of Arabidopsis AtNHX1 negatively regulates the Na(+)/H(+) transporting activity. In this study, we truncated the hydrophilic C terminus of a vacuolar Na(+) /H(+) antiporter gene from Salicornia europaea (SeNHX1) to generate its derivative, SeNHX1-ΔC. Expression of SeNHX1 and SeNHX1-ΔC in yeast mutant showed that SeNHX1 significantly improved the tolerance to NaCl; however, the expression of SeNHX1-ΔC enormously decreased the tolerance to NaCl. Overall, these results suggest that the hydrophilic C-terminal region of SeNHX1 is required for Na(+)/H(+) exchanging activity of SeNHX1.
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China. wanggangtjdx@126.com.
authors.forename: Guangxia
authors.initials: G
authors.lastname: Wu
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Ji
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Tian
authors.forename: Hailing
authors.initials: H
authors.lastname: Gao
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Yurong
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D019663:Chenopodiaceae / Q000235:genetics* / Q000502:physiology; D005816:Genetic Complementation Test; D057927:Hydrophobic and Hydrophilic Interactions; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000235:genetics; D055049:Salt Tolerance; D017386:Sequence Homology, Amino Acid; D017923:Sodium-Hydrogen Exchangers / Q000737:chemistry* / Q000502:physiology
chemical_list: D017923:Sodium-Hydrogen Exchangers