title: Purification, crystallization and preliminary X-ray crystallographic analysis of glycosyltransferase-1 from Bacillus cereus.
abstract: Glycosyltransferases (GTs), which are distributed widely in various organisms, including bacteria, fungi, plants and animals, play a role in synthesizing biological compounds. Glycosyltransferase-1 from Bacillus cereus (BcGT-1), which is capable of transferring glucose to small molecules such as kaempferol and quercetin, has been identified as a member of the family 1 glycosyltransferases which utilize uridine diphosphate glucose (UDP-glucose) as the sugar donor. BcGT-1 (molecular mass 45.5 kDa) has been overexpressed, purified and crystallized using the hanging-drop vapour-diffusion method. According to X-ray diffraction of BcGT-1 crystals to 2.10 Å resolution, the crystal belonged to space group P1, with unit-cell parameters a = 54.56, b = 84.81, c = 100.12 Å, α = 78.36, β = 84.66, γ = 84.84°. Preliminary analysis indicates the presence of four BcGT-1 molecules in the asymmetric unit with a solvent content of 50.27%.
authors.affiliation: Life Science Group, Scientific Research Division, National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan.
authors.forename: Yin-Cheng
authors.initials: YC
authors.lastname: Hsieh
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu 30010, Taiwan.
authors.forename: Hsi-Ho
authors.initials: HH
authors.lastname: Chiu
authors.affiliation: Life Science Group, Scientific Research Division, National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan.
authors.forename: Yen-Chieh
authors.initials: YC
authors.lastname: Huang
authors.affiliation: Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Hoong-Kun
authors.initials: HK
authors.lastname: Fun
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu 30010, Taiwan.
authors.forename: Chia-Yu
authors.initials: CY
authors.lastname: Lu
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu 30010, Taiwan.
authors.forename: Yaw-Kuen
authors.initials: YK
authors.lastname: Li
authors.affiliation: Life Science Group, Scientific Research Division, National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan.
authors.forename: Chun-Jung
authors.initials: CJ
authors.lastname: Chen
mesh_terms: D001409:Bacillus cereus / Q000201:enzymology*; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D016695:Glycosyltransferases / Q000737:chemistry*
chemical_list: D016695:Glycosyltransferases
keywords: Bacillus cereus; glycosyltransferase