title: Molecular diversity of Scottish Cryptosporidium hominis isolates.
abstract: Cryptosporidium hominis is one of the most prevalent protozoan parasites to infect humans where transmission is via the consumption of infective oocysts. This study describes sporadic cases in addition to the molecular diversity of outbreak cases in Scotland using the glycoprotein-60 subtyping tool. From a total of 187 C. hominis isolates, 65 were subjected to further molecular analysis and 46 were found to be the common IbA10G2 subtype. Unusual subtypes included four isolates belonging to the Ia family (IaA14R3, n = 12; IaA14R2, n = 1; IaA9G3, n = 1; IaA25R3, n = 2), two from the Id family (IdA24, n = 1; IdA17, n = 1) and one belonging to the Ie family, namely IeA11G3T3. These data contribute significantly to our knowledge and understanding of the molecular diversity of C. hominis isolates from outbreak investigations involving Scottish residents which will be beneficial for the management of future outbreaks.
authors.affiliation: Clinical Microbiology,Greater Glasgow and Clyde NHS.
authors.forename: A
authors.initials: A
authors.lastname: Deshpande
authors.affiliation: Scottish Microbiology Reference Laboratories,Greater Glasgow and Clyde NHS.
authors.forename: C L
authors.initials: CL
authors.lastname: Alexander
authors.affiliation: Scottish Microbiology Reference Laboratories,Greater Glasgow and Clyde NHS.
authors.forename: M
authors.initials: M
authors.lastname: Coyne
authors.affiliation: Health Protection Scotland,NHS National Services Scotland.
authors.forename: S
authors.initials: S
authors.lastname: Brownlie
authors.affiliation: Health Protection Scotland,NHS National Services Scotland.
authors.forename: A
authors.initials: A
authors.lastname: Smith-Palmer
authors.affiliation: Scottish Microbiology Reference Laboratories,Greater Glasgow and Clyde NHS.
authors.forename: B L
authors.initials: BL
authors.lastname: Jones
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D002648:Child; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology; D003458:Cryptosporidium / Q000235:genetics*; D004196:Disease Outbreaks / Q000706:statistics & numerical data; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008875:Middle Aged; D012606:Scotland / Q000453:epidemiology; D055815:Young Adult
keywords: outbreaks