title: Features of cellular effects of combined exposure to ionizing radiation and copper ions.
abstract: The constant increase in the scale of the use of chemicals and ionizing radiation in various industries, medicine and science increases their impact on all environmental components. Therefore, the probability of simultaneous effects of radiation and chemical factors on biological objects is growing. In this regard the issue of combined exposure to different by virtue of the nature factors is becoming more and more challenging. The objective of the work was to investigate the characteristics of the individual and combined effects of ionizing radiation and copper salts on cell viability in vitro.
  
  
  METHODS
  The research involved inoculated cell culture line L929. Copper acetate (Cu(CH₃COOH)₂) was added to the cell culture 1 hour after irradiation. Irradiation of the cells was performed 24 hours after passaging. Proliferative activity of the cells was assessed by growth kinetics. Study results showed the cytotoxicity of copper ions at concentration of 1 μmol/L. A combined effect of ionizing radiation in sublethal doses and copper ions at a concentration of 0.001 and 1 μmol/L led to the increased number of atypical polykaryocytes.
  
  
  CONCLUSIONS
  Both activation and inhibition of cell proliferation and mitotic activity occurs after the incubation of cells with copper ions at various concentrations. Cellular survival and mitotic activity decrease along with radiation dose elevation. Index of polykaryocytes at that is elevated. The altered morphological and functional properties of the cells were found in combined exposure to ionizing radiation and copper ions in vitro. They were not directly proportional either to a dose of irradiation or concentration of copper ions.
authors.forename: D D
authors.initials: DD
authors.lastname: Hapieienko
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003300:Copper / Q000494:pharmacology* / Q000633:toxicity; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D051379:Mice; D008940:Mitotic Index; D011829:Radiation Dosage; D011839:Radiation, Ionizing
chemical_list: D003300:Copper
keywords: cell culture; heavy metals; ionizing radiation; mitosis; proliferation; survival