title: Cloning and characterization of an apolipoprotein C2 promoter in the mouse central nervous system.
abstract: Apolipoprotein C2 is an important member of the apolipoprotein C family, and is a potent activator of lipoprotein lipase. In the central nervous system, apolipoprotein C2 plays an important role in the catabolism of triglyceride-rich lipoproteins. Studies into the exact regulatory mechanism of mouse apolipoprotein C2 expression have not been reported. In this study, seven luciferase expression vectors, which contained potential mouse apolipoprotein C2 gene promoters, were constructed and co-transfected with pRL-TK into HEK293T cells to investigate apolipoprotein C2 promoter activity. Luciferase assays indicated that the apolipoprotein C2 promoter region was mainly located in the +104 bp to +470 bp region. The activity of the different lengths of apolipoprotein C2 promoter region varied. This staggered negative-positive-negative arrangement indicates the complex regulation of apolipoprotein C2 expression and provides important clues for elucidating the regulatory mechanism of apolipoprotein C2 gene transcription.
authors.affiliation: Key Laboratory of Transgenetic Animal Research, Department of Laboratory Animal, China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Transgenetic Animal Research, Department of Laboratory Animal, China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Du
authors.affiliation: Key Laboratory of Transgenetic Animal Research, Department of Laboratory Animal, China Medical University, Shenyang 110001, Liaoning Province, China ; College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, Liaoning Province, China.
authors.forename: Shengyang
authors.initials: S
authors.lastname: Li
authors.affiliation: Key Laboratory of Transgenetic Animal Research, Department of Laboratory Animal, China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Xiangchuan
authors.initials: X
authors.lastname: Lv
authors.affiliation: Key Laboratory of Transgenetic Animal Research, Department of Laboratory Animal, China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Shenglai
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Transgenetic Animal Research, Department of Laboratory Animal, China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Key Laboratory of Transgenetic Animal Research, Department of Laboratory Animal, China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Transgenetic Animal Research, Department of Laboratory Animal, China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zheng
keywords: apolipoprotein C2; basic research; dual-luciferase reporter assay; grants-supported paper; neural regeneration; neuroregeneration; promoter; regulatory elements; transcriptional activity