title: [Effect on zebrafish neurodevelopment and neuroprotection of a polyphenolic extract olive seeds].
abstract: OBJECTIVE
  To determine the effect of a polyphenolic extract from olive pit on the development of the nervous system as well as its effect on pain induced by the neurotoxin kainic acid, taking the zebrafish as the animal model.
  
  
  METHODS
  We analyse the effect of the extract at the maximum tolerated dose (100 mg/ml of polyphenols) on the cholinergic activity in zebrafish larvae (72 hours post-fertilization). Only fecundated eggs with no abnormalities are used. 6 eggs/bowl are incubated in a 24 bowls microplate in 2 ml of water with DMSO (0.1%) at 26 ± 1º C: a) neurodevelopment: water (control) and 100 mg/ml of extract, as an essay; b) neuroprotection: water and kainic acid (100 μM) (control) and 100 mg/ml of extract (essay). All incubations are in triplicate. After 72 h, incubations are examined and checked for any abnormalities. Larvae are homogenized and acetyl cholinesterase activity and protein concentration in supernatants is quantified.
  
  
  RESULTS
  The quantity of protein and the morphologic appreciation is similar in all the essays, showing a standard development. Acetyl cholinesterase in fish larvae, with the polyphenolic extract is 162.2% (SD 44.2) compared to controls (100% of activity) (p < 0.01). Fish larvae treated with kainic acid and polyphenolic acid show 140.1% (SD 22.0) of activity, compared to those only incubated with the neurotoxin (100%) (p < 0.05).
  
  
  CONCLUSIONS
  Polyphenols extracted from olive pit produce an increase in the cholinergic activity during the larvae neurodevelopment in the zebrafish as well as protection against the neurotoxin kainic acid.
authors.affiliation: Departamento de Farmacología, Pediatría y Q. Orgánica. Universidad Miguel Hernández. Campus de San Juan. Alicante. España.. ernesto.cortes@umh.es.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Cortés Castell
authors.affiliation: Laboratorio de Biotecnología y Proyectos. Biopartner S.L.. cveciana@biopartner.net.
authors.forename: C
authors.initials: C
authors.lastname: Veciana Galindo
authors.affiliation: Laboratorio de Biotecnología y Proyectos. Biopartner S.L.. ltorro@biopartner.net.
authors.forename: L
authors.initials: L
authors.lastname: Torro Montell
authors.affiliation: Laboratorio de Biotecnología y Proyectos. Biopartner S.L.. esirvent@biopartner.net.
authors.forename: E
authors.initials: E
authors.lastname: Sirvent Segura
authors.affiliation: Departamento de Enfermería y Nutrición. Universidad de Alicante.. mercedes.rizo@ua.es.
authors.forename: M M
authors.initials: MM
authors.lastname: Rizo Baeza
authors.affiliation: Departamento de Medicina Clínica. Universidad Miguel Hernández. España.. vtgil@umh.es.
authors.forename: V
authors.initials: V
authors.lastname: Gil Guillén
mesh_terms: D000818:Animals; D007608:Kainic Acid / Q000008:administration & dosage; D009420:Nervous System / Q000187:drug effects* / Q000254:growth & development*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D031658:Olea; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D059808:Polyphenols; D012639:Seeds; D015027:Zebrafish / Q000254:growth & development*
chemical_list: D018696:Neuroprotective Agents; D010936:Plant Extracts; D059808:Polyphenols; D007608:Kainic Acid