title: Enterococcus faecium PBP5-S/R, the missing link between PBP5-S and PBP5-R.
abstract: During a study to investigate the evolution of ampicillin resistance in Enterococcus faecium, we observed that a number of E. faecium strains, mainly from the recently described subclade A2, showed PBP5 sequences in between PBP5-S and PBP5-R. These hybrid PBP5-S/R patterns reveal a progression of amino acid changes from the S form to the R form of this protein; however, these changes do not strictly correlate with changes in ampicillin MICs.
authors.affiliation: Division of Infectious Diseases, University of Texas Medical School, Houston, Texas, USA Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Piacenza, Cremona, Italy.
authors.forename: Ester
authors.initials: E
authors.lastname: Pietta
authors.affiliation: Division of Infectious Diseases, University of Texas Medical School, Houston, Texas, USA Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston, Texas, USA.
authors.forename: Maria Camila
authors.initials: MC
authors.lastname: Montealegre
authors.affiliation: Division of Infectious Diseases, University of Texas Medical School, Houston, Texas, USA.
authors.forename: Jung Hyeob
authors.initials: JH
authors.lastname: Roh
authors.affiliation: Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Piacenza, Cremona, Italy.
authors.forename: Pier Sandro
authors.initials: PS
authors.lastname: Cocconcelli
authors.affiliation: Division of Infectious Diseases, University of Texas Medical School, Houston, Texas, USA Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston, Texas, USA Center for the Study of Emerging and Re-emerging Pathogens, University of Texas Medical School, Houston, Texas, USA bem.asst@uth.tmc.edu.
authors.forename: Barbara E
authors.initials: BE
authors.lastname: Murray
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000667:Ampicillin / Q000494:pharmacology; D000668:Ampicillin Resistance / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016984:Enterococcus faecium / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics*; D014644:Genetic Variation; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D046915:Penicillin-Binding Proteins / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; C569173:PBP5 protein, E coli; D046915:Penicillin-Binding Proteins; D020033:Protein Isoforms; D000667:Ampicillin