title: MALDI-TOF MS based identification of food-borne yeast isolates.
abstract: In this study, food-borne yeast isolates (n=96), comprising at least 33 species, were identified using MALDI-TOF MS and conventional methods (API ID 32 C and Phoenix Yeast ID). Discrepancies of both methods were resolved by sequencing the ITS1-5.8S-rRNA-ITS2 region. For ten isolates, mainly classified to Rhodotorula and Trichosporon species, no clear final species identification was possible. 62 isolates were correctly identified to species level using either MALDI-TOF MS or conventional tests. 15 isolates were misidentified when applying conventional assays. In contrary, no species misidentifications were observed after MALDI-TOF MS based classification. In return, 16 isolates were not identifiable after matching their protein fingerprints against MALDI Biotyper 4.0.0.1 library. MALDI TOF MS in-house database update clearly improved the identification. In conclusion, the presented data suggest that MALDI-TOF MS is an appropriate platform for reliable classification and identification of food-borne yeast isolates.
authors.affiliation: Bavarian Health and Food Safety Authority, Veterinaerstr. 2, 85764 Oberschleißheim, Germany. Electronic address: Melanie.Pavlovic@lgl.bayern.de.
authors.forename: Melanie
authors.initials: M
authors.lastname: Pavlovic
authors.affiliation: Bavarian Health and Food Safety Authority, Veterinaerstr. 2, 85764 Oberschleißheim, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Mewes
authors.affiliation: Bavarian Health and Food Safety Authority, Veterinaerstr. 2, 85764 Oberschleißheim, Germany.
authors.forename: Marzena
authors.initials: M
authors.lastname: Maggipinto
authors.affiliation: Bavarian Health and Food Safety Authority, Veterinaerstr. 2, 85764 Oberschleißheim, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schmidt
authors.affiliation: Bavarian Health and Food Safety Authority, Veterinaerstr. 2, 85764 Oberschleißheim, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Messelhäußer
authors.affiliation: Bavarian Health and Food Safety Authority, Eggenreuther Weg 43, 91058 Erlangen, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Balsliemke
authors.affiliation: Bavarian Health and Food Safety Authority, Veterinaerstr. 2, 85764 Oberschleißheim, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Hörmansdorfer
authors.affiliation: Bavarian Health and Food Safety Authority, Veterinaerstr. 2, 85764 Oberschleißheim, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Busch
authors.affiliation: Bavarian Health and Food Safety Authority, Veterinaerstr. 2, 85764 Oberschleißheim, Germany.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Huber
mesh_terms: D005516:Food Microbiology; D008828:Microbiological Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D015003:Yeasts / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification*
keywords: Food microbiology; Food-borne; MALDI Biotyper; MALDI-TOF MS; Yeast