title: In vivo interactions between procyanidins and human saliva proteins: effect of repeated exposures to procyanidins solution.
abstract: The general accepted mechanism for astringency arises from the interaction between tannins and salivary proteins (SP) resulting in (in)soluble aggregates. By HPLC analysis, it was observed that repeated sips of procyanidins (PC) solution practically depleted aPRPs (∼14%) and statherin (∼2%), and significantly reduced the amount of gPRPs. On the other hand, bPRPs were not significantly affected. In the analysis performed after the last exposure to PC solution, it was seen a significant recovering of the chromatographic peaks corresponding especially to aPRPs (∼74%) and statherin (∼80%). In vitro interaction between SP and PC results in the decrease of the chromatographic peaks of aPRPs and statherin, suggesting that these proteins were involved in the formation of a significant quantity of insoluble complexes. In general, the results suggest that the different families of SP can be involved in different stages of the development of astringency sensation.
authors.affiliation: Centro de Investigação em Química (CIQ), Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto , Rua do Campo Alegre 687, 4169-007 Porto, Portugal.
authors.forename: Elsa
authors.initials: E
authors.lastname: Brandão
authors.forename: Susana
authors.initials: S
authors.lastname: Soares
authors.forename: Nuno
authors.initials: N
authors.lastname: Mateus
authors.forename: Victor
authors.initials: V
authors.lastname: de Freitas
mesh_terms: D000328:Adult; D044946:Biflavonoids / Q000737:chemistry*; D002392:Catechin / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D044945:Proanthocyanidins / Q000737:chemistry*; D012463:Saliva / Q000737:chemistry*; D055273:Salivary Proline-Rich Proteins / Q000737:chemistry*; D013649:Taste; D055815:Young Adult
chemical_list: D044946:Biflavonoids; D044945:Proanthocyanidins; D055273:Salivary Proline-Rich Proteins; C017674:procyanidin; D002392:Catechin
keywords: astringency; complexes; procyanidins; proline-rich proteins; protein−procyanidins interaction; statherin