title: Adipogenic differentiation potential of adipose-derived mesenchymal stem cells from ovariectomized mice.
abstract: OBJECTIVE
  In human post-menopausal osteoporosis, enhanced adipogenesis in bone marrow and enhanced formation of adipose tissue in vivo are observed. These changes correlate with reduced trabecular bone volume and increased adipocyte cell size as well as cell number. However, cellular and molecular mechanisms underlying osteoporosis-related changes in adipocyte cell volume are not known. This study was designed to compare adipogenic potential of adipose tissue-derived stem cells (ADSCs) obtained from ovariectomized mice with that of control ADSCs, and to analyse pathological mechanisms from the point of functional changes of ADSCs.
  
  
  METHODS
  Healthy female C57BL/6J mice were randomly divided into ovariectomy and sham-surgery groups. Mouse ADSCs were isolated and cultured in vitro up to passage 3. After adipogenic induction, oil red O staining of lipid droplets was used to detect adipogenic ability of ADSCs; real-time PCR and immunofluorescence staining were used to detect expression of adipogenesis-related genes and proteins.
  
  
  RESULTS
  As indicated by increased expression of adipogenic and lipogenic genes and proteins, and lipid droplets accumulation shown by oil red-O staining, adipogenic differentiation of ADSCs was significantly enhanced in the ovariectomy group compared to the sham-surgery group (P < 0.05).
  
  
  CONCLUSIONS
  These findings suggest that enhanced adipogenic differentiation of ADSCs is likely to be the important cause for increased adipogenesis in vivo and subsequent obesity-like changes in body mass, in mice, after ovariectomy.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan Province, 610041, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Fu
authors.forename: R
authors.initials: R
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Zhong
authors.forename: N
authors.initials: N
authors.lastname: Fu
authors.forename: X
authors.initials: X
authors.lastname: Wei
authors.forename: X
authors.initials: X
authors.lastname: Cun
authors.forename: S
authors.initials: S
authors.lastname: Deng
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Xie
authors.forename: X
authors.initials: X
authors.lastname: Cai
authors.forename: Y
authors.initials: Y
authors.lastname: Lin
mesh_terms: D052242:Adiponectin / Q000235:genetics / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D050356:Lipid Metabolism; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010052:Ovariectomy; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D052242:Adiponectin; D047495:PPAR gamma; D012333:RNA, Messenger; D008071:Lipoprotein Lipase