title: Phylogenetic diversity of dominant bacterial and archaeal communities in plant-microbial fuel cells using rice plants.
abstract: In this study, the phylogenetic diversities of bacterial and archaeal communities in a plantmicrobial fuel cell (P-MFC) were investigated together with the environmental parameters, affecting its performance by using rice as a model plant. The beneficial effect of the plant appeared only during a certain period of the rice-growing season, at which point the maximum power density was approximately 3-fold higher with rice plants. The temperature, electrical conductivity (EC), and pH in the cathodic and anodic compartments changed considerably during the rice-growing season, and a higher temperature, reduced difference in pH between the cathodic and anodic compartments, and higher EC were advantageous to the performance of the P-MFC. A 16S rRNA pyrosequencing analysis showed that the 16S rRNAs of Deltaproteobacteria and those of Gammaproteobacteria were enriched on the anodes and the cathodes, respectively, when the electrical circuit was connected. At the species level, the operational taxonomic units (OTUs) related to Rhizobiales, Geobacter, Myxococcus, Deferrisoma, and Desulfobulbus were enriched on the anodes, while an OTU related to Acidiferrobacter thiooxydans occupied the highest proportion on the cathodes and occurred only when the circuit was connected. Furthermore, the connection of the electrical circuit decreased the abundance of 16S rRNAs of acetotrophic methanogens and increased that of hydrogenotrophic methanogens. The control of these physicochemical and microbiological factors is expected to be able to improve the performance of P-MFCs.
authors.affiliation: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Wanju, 565-851, Republic of Korea.
authors.forename: Jae-Hyung
authors.initials: JH
authors.lastname: Ahn
authors.forename: Woo-Suk
authors.initials: WS
authors.lastname: Jeong
authors.forename: Min-Young
authors.initials: MY
authors.lastname: Choi
authors.forename: Byung-Yong
authors.initials: BY
authors.lastname: Kim
authors.forename: Jaekyeong
authors.initials: J
authors.lastname: Song
authors.forename: Hang-Yeon
authors.initials: HY
authors.lastname: Weon
mesh_terms: D001105:Archaea / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D058448:Biota; D016000:Cluster Analysis; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004553:Electric Conductivity; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D012275:Oryza / Q000254:growth & development* / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S