title: Genome sequence of Rhizobium leguminosarum bv trifolii strain WSM1689, the microsymbiont of the one flowered clover Trifolium uniflorum.
abstract: Rhizobium leguminosarum bv. trifolii is a soil-inhabiting bacterium that has the capacity to be an effective N2-fixing microsymbiont of Trifolium (clover) species. R. leguminosarum bv. trifolii strain WSM1689 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a root nodule of Trifolium uniflorum collected on the edge of a valley 6 km from Eggares on the Greek Island of Naxos. Although WSM1689 is capable of highly effective N2-fixation with T. uniflorum, it is either unable to nodulate or unable to fix N2 with a wide range of both perennial and annual clovers originating from Europe, North America and Africa. WSM1689 therefore possesses a very narrow host range for effective N2 fixation and can thus play a valuable role in determining the geographic and phenological barriers to symbiotic performance in the genus Trifolium. Here we describe the features of R. leguminosarum bv. trifolii strain WSM1689, together with the complete genome sequence and its annotation. The 6,903,379 bp genome contains 6,709 protein-coding genes and 89 RNA-only encoding genes. This multipartite genome contains six distinct replicons; a chromosome of size 4,854,518 bp and five plasmids of size 667,306, 518,052, 341,391, 262,704 and 259,408 bp. This rhizobial genome is one of 20 sequenced as part of a DOE Joint Genome Institute 2010 Community Sequencing Program.
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Perth, Australia.
authors.forename: Jason
authors.initials: J
authors.lastname: Terpolilli
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Perth, Australia.
authors.forename: Tian
authors.initials: T
authors.lastname: Rui
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Perth, Australia ; Department of Agriculture and Food, Western Australia, Australia.
authors.forename: Ron
authors.initials: R
authors.lastname: Yates
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Perth, Australia.
authors.forename: John
authors.initials: J
authors.lastname: Howieson
authors.affiliation: Department of Plant Sciences, University of Oxford, UK ; Sir Walter Murdoch Adjunct Professor, Murdoch University, Perth, Australia.
authors.forename: Philip
authors.initials: P
authors.lastname: Poole
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors.forename: Christine
authors.initials: C
authors.lastname: Munk
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors.forename: Roxanne
authors.initials: R
authors.lastname: Tapia
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors.forename: Cliff
authors.initials: C
authors.lastname: Han
authors.affiliation: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Victor
authors.initials: V
authors.lastname: Markowitz
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Reddy
authors.initials: R
authors.lastname: Tatiparthi
authors.affiliation: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Mavrommatis
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Ivanova
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Amrita
authors.initials: A
authors.lastname: Pati
authors.affiliation: Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
authors.forename: Lynne
authors.initials: L
authors.lastname: Goodwin
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Tanja
authors.initials: T
authors.lastname: Woyke
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California, USA.
authors.forename: Nikos
authors.initials: N
authors.lastname: Kyrpides
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Perth, Australia.
authors.forename: Wayne
authors.initials: W
authors.lastname: Reeve
keywords: Alphaproteobacteria; lupin-nodulating; nitrogen fixation; rhizobia; root-nodule bacteria