title: Interleukin-27 inhibits foam cell formation by promoting macrophage ABCA1 expression through JAK2/STAT3 pathway.
abstract: The purpose of this study is to determine whether IL-27 regulates macrophage ABCA1 expression, foam cell formation, and also explore the underlying mechanisms. Here, we revealed that IL-27 decreased lipid accumulation in THP-1 derived macrophages through markedly enhancing cholesterol efflux and increasing ABCA1 expression at both protein and mRNA levels. Our study further demonstrated that IL-27 increased ABCA1 level via activation of signal transducer and activator of transcription 3 (STAT3). Inhibition of Janus kinase 2, (JAK2)/STAT3 suppressed the stimulatory effects of IL-27 on ABCA1 expression. The present study concluded that IL-27 reduces lipid accumulation of foam cell by upregulating ABCA1 expression via JAK2/STAT3. Therefore, targeting IL-27 may offer a promising strategy to treat atherosclerotic vascular disease.
authors.affiliation: Department of ICU, First Affiliated Hospital of University of South China, Hengyang 421001, Hunan, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Fu
authors.affiliation: Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Tang
authors.affiliation: Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Xin-Ping
authors.initials: XP
authors.lastname: Ouyang
authors.affiliation: Department of ICU, First Affiliated Hospital of University of South China, Hengyang 421001, Hunan, China.
authors.forename: Shi-Lin
authors.initials: SL
authors.lastname: Tang
authors.affiliation: Department of ICU, First Affiliated Hospital of University of South China, Hengyang 421001, Hunan, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Su
authors.affiliation: Department of ICU, First Affiliated Hospital of University of South China, Hengyang 421001, Hunan, China.
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of ICU, First Affiliated Hospital of University of South China, Hengyang 421001, Hunan, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Huang
authors.affiliation: Department of ICU, First Affiliated Hospital of University of South China, Hengyang 421001, Hunan, China.
authors.forename: Miao
authors.initials: M
authors.lastname: He
authors.affiliation: Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Yun-Cheng
authors.initials: YC
authors.lastname: Lv
authors.affiliation: Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Ping-Ping
authors.initials: PP
authors.lastname: He
authors.affiliation: Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yao
authors.affiliation: Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Tan
authors.affiliation: Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xie
authors.affiliation: Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Cardiovascular Medicine, Second Affiliated Hospital of University of South China, Hengyang 421001, Hunan, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Wu
authors.affiliation: Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Cardiovascular Medicine, Second Affiliated Hospital of University of South China, Hengyang 421001, Hunan, China.
authors.forename: Kong
authors.initials: K
authors.lastname: Chen
authors.affiliation: Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Lan
authors.affiliation: Department of Cardiovascular Medicine, Second Affiliated Hospital of University of South China, Hengyang 421001, Hunan, China.
authors.forename: Meng-Ya
authors.initials: MY
authors.lastname: Zeng
authors.affiliation: Department of Biochemistry and Molecular Biology, The Libin Cardiovascular Institute of Alberta, The University of Calgary, Health Sciences Center, 3330 Hospital Dr. NW, Calgary, Alberta T2N 4N1, Canada.
authors.forename: Xi-Long
authors.initials: XL
authors.lastname: Zheng
authors.affiliation: Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, University of South China, Hengyang, Hunan 421001, China. Electronic address: tangchaoke@qq.com.
authors.forename: Chao-Ke
authors.initials: CK
authors.lastname: Tang
mesh_terms: D064286:ATP Binding Cassette Transporter 1 / Q000378:metabolism*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D002784:Cholesterol / Q000378:metabolism*; D005487:Foam Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D064094:Interleukin-27 / Q000494:pharmacology*; D053614:Janus Kinase 2 / Q000378:metabolism*; D050356:Lipid Metabolism / Q000502:physiology*; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
chemical_list: C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D064094:Interleukin-27; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D002784:Cholesterol; C507924:JAK2 protein, human; D053614:Janus Kinase 2
keywords: ATP-binding cassette transporter A1; Foam cell; Interleukin-27; Janus kinase 2; Signal transducer and activator of transcription 3