title: Optimization of ultrasound-assisted extraction parameters of chlorophyll from Chlorella vulgaris residue after lipid separation using response surface methodology.
abstract: An investigation into ultrasound-assisted extraction (UAE) was conducted for the extraction of chlorophyll from Chlorella vulgaris residue after lipid separation. The best possible combination of extraction parameters was obtained with the response surface methodology (RSM), at a three-variable, three-level experiment Box-Behnken design (BBD). The optimum extraction parameters were as follows: extraction temperature, 61.4 °C, extraction time, 78.7 min, ethanol volume, 79.4 %, at a fixed ultrasonic power of 200 W. Under the modified optimal conditions, the model predicted a total chlorophyll content of 30.1 mg/g. Verification of the optimization showed that chlorophyll extraction of 31.1 ± 1.56 mg/g was observed under the optimal conditions, which well matches with the predicted value. Under these conditions, two stage extraction could sufficiently reach the maximal chlorophyll yield (35.2 mg/g), and the extraction rate reached up to 88.9 %. The present paper provides a feasible technology route for comprehensive utilization of bioactive substances from Chlorella and microalgal biomass biorefinery.
authors.affiliation: College of Life Science, Northwest Normal University, Lanzhou, 730070 China ; State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000 China.
authors.forename: Weibao
authors.initials: W
authors.lastname: Kong
authors.affiliation: College of Life Science, Northwest Normal University, Lanzhou, 730070 China.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.affiliation: College of Life Science, Northwest Normal University, Lanzhou, 730070 China.
authors.forename: Ji
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Life Science, Northwest Normal University, Lanzhou, 730070 China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Yang
authors.affiliation: State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000 China.
authors.forename: Shaofeng
authors.initials: S
authors.lastname: Hua
authors.affiliation: State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000 China.
authors.forename: Hao
authors.initials: H
authors.lastname: Song
authors.affiliation: State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000 China.
authors.forename: Chungu
authors.initials: C
authors.lastname: Xia
keywords: Chlorella vulgaris; Chlorophyll; Optimization; Response surface methodology; Ultrasound-assisted extraction