title: Development of genomic microsatellites in Gleditsia triacanthos (Fabaceae) using Illumina sequencing.
abstract: OBJECTIVE
  Fourteen genomic microsatellite markers were developed and characterized in honey locust, Gleditsia triacanthos, using Illumina sequencing. Due to their high variability, these markers can be applied in analyses of genetic diversity and structure, and in mating system and gene flow studies. •
  
  
  RESULTS
  Thirty-six individuals from across the species range were included in a genetic diversity analysis and yielded three to 20 alleles per locus. Observed heterozygosity and expected heterozygosity ranged from 0.214 to 0.944 and from 0.400 to 0.934, respectively, with minimal occurrence of null alleles. Regular segregation of maternal alleles was observed at seven loci and moderate segregation distortion at four of 11 loci that were heterozygous in the seed parent. •
  
  
  CONCLUSIONS
  Honey locust is an important agroforestry tree capable of very fast growth and tolerance of poor site conditions. This is the first report of genomic microsatellites for this species.
authors.affiliation: School of Forest Resources and Environmental Science, Michigan Technological University, 1400 Townsend Drive, Houghton, Michigan 49931 USA.
authors.forename: Sandra A
authors.initials: SA
authors.lastname: Owusu
authors.affiliation: Clemson University Genomics Institute, Clemson, South Carolina 29634 USA.
authors.forename: Margaret
authors.initials: M
authors.lastname: Staton
authors.affiliation: Pacific Northwest Research Station, USDA Forest Service, Corvallis, Oregon 97331 USA.
authors.forename: Tara N
authors.initials: TN
authors.lastname: Jennings
authors.affiliation: Department of Forestry, Wildlife and Fisheries, University of Tennessee, Knoxville, Tennessee 37996-4563 USA.
authors.forename: Scott
authors.initials: S
authors.lastname: Schlarbaum
authors.affiliation: School of Natural Resources, University of Missouri, Columbia, Missouri 65211 USA.
authors.forename: Mark V
authors.initials: MV
authors.lastname: Coggeshall
authors.affiliation: Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556 USA.
authors.forename: Jeanne
authors.initials: J
authors.lastname: Romero-Severson
authors.affiliation: Department of Ecosystem Science and Management and Department of Plant Science, Pennsylvania State University, University Park, Pennsylvania 16802 USA.
authors.forename: John E
authors.initials: JE
authors.lastname: Carlson
authors.affiliation: School of Forest Resources and Environmental Science, Michigan Technological University, 1400 Townsend Drive, Houghton, Michigan 49931 USA.
authors.forename: Oliver
authors.initials: O
authors.lastname: Gailing
keywords: Fabaceae; Gleditsia triacanthos; agroforestry; microsatellite; next-generation sequencing