title: Development and evaluation of microsatellite markers for the gynodioecious shrub Daphne jezoensis (Thymelaeaceae).
abstract: OBJECTIVE
  Ten microsatellite markers were developed and characterized in a gynodioecious summer-deciduous shrub, Daphne jezoensis, to facilitate studies of the evolution of gynodioecy in the species. •
  
  
  RESULTS
  We used a next-generation sequencing approach with the Ion Personal Genome Machine (PGM) system to identify and develop microsatellite markers with perfect di- and trinucleotide repeats. These markers were tested with 47 samples from two natural populations. The mean observed and expected heterozygosities per population ranged from 0.40 to 0.46 and 0.60 to 0.66, respectively. •
  
  
  CONCLUSIONS
  The developed markers will be useful to study the mating system, gene flow, and population genetic structure of D. jezoensis.
authors.affiliation: Faculty of Regional Environment Science, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kameyama
authors.affiliation: Sugadaira Montane Research Center, University of Tsukuba, 1278-294 Sugadairakogen, Ueda-shi, Nagano-ken 386-2204, Japan.
authors.forename: Akira S
authors.initials: AS
authors.lastname: Hirao
keywords: Daphne jezoensis; Thymelaeaceae; gynodioecy; microsatellite