title: Peptides derived from Rhopilema esculentum hydrolysate exhibit angiotensin converting enzyme (ACE) inhibitory and antioxidant abilities.
abstract: Jellyfish (Rhopilema esculentum) was hydrolyzed using alcalase, and two peptides with angiotensin-I-converting enzyme (ACE) inhibitory and antioxidant activities were purified by ultrafiltration and consecutive chromatographic methods. The amino acid sequences of the two peptides were identified as VKP (342 Da) and VKCFR (651 Da) by electrospray ionization tandem mass spectrometry. The IC50 values of ACE inhibitory activities of the two peptides were 1.3 μM and 34.5 μM, respectively. Molecular docking results suggested that VKP and VKCFR bind to ACE through coordinating with the active site Zn(II) atom. Free radical scavenging activity and protection against hydrogen peroxide (H2O2)-induced rat cerebral microvascular endothelial cell (RCMEC) injury were used to evaluate the antioxidant activities of the two peptides. As the results clearly showed that the peptides increased the superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-px) activities in RCMEC cells), it is proposed that the R. esculentum peptides exert significant antioxidant effects.
authors.affiliation: State Key Laboratory of Reproductive Medicine, Department of Plastic & Consmetic Surgery, Nanjing Maternity and Child Health Hospital, Nanjing Medical University, Nanjing 210029, China. drlijun@126.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Reproductive Medicine, Department of Plastic & Consmetic Surgery, Nanjing Maternity and Child Health Hospital, Nanjing Medical University, Nanjing 210029, China. drliqian@126.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: State Key Laboratory of Reproductive Medicine, Department of Plastic & Consmetic Surgery, Nanjing Maternity and Child Health Hospital, Nanjing Medical University, Nanjing 210029, China. lijingyun175@gmail.com.
authors.forename: Jingyun
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Reproductive Medicine, Department of Plastic & Consmetic Surgery, Nanjing Maternity and Child Health Hospital, Nanjing Medical University, Nanjing 210029, China. enzhoubei@126.com.
authors.forename: Bei
authors.initials: B
authors.lastname: Zhou
mesh_terms: D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D006868:Hydrolysis; D062105:Molecular Docking Simulation; D010446:Peptide Fragments / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D007703:Peptidyl-Dipeptidase A / Q000737:chemistry; D051381:Rats; D037401:Scyphozoa / Q000737:chemistry
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D016166:Free Radical Scavengers; D010446:Peptide Fragments; D007703:Peptidyl-Dipeptidase A