title: PPARA intron polymorphism associated with power performance in 30-s anaerobic Wingate Test.
abstract: To date, polymorphisms in several genes have been associated with a strength/power performance including alpha 3 actinin, ciliary neurotrophic factor, vitamin D receptor, or angiotensin I converting enzyme, underlining the importance of genetic component of the multifactorial strength/power-related phenotypes. The single nucleotide variation in peroxisome proliferator-activated receptor alpha gene (PPARA) intron 7 G/C (rs4253778; g.46630634G>C) has been repeatedly found to play a significant role in response to different types of physical activity. We investigated the effect of PPARA intron 7 G/C polymorphism specifically on anaerobic power output in a group of 77 elite male Czech ice hockey players (18-36 y). We determined the relative peak power per body weight (Pmax.kg(-1)) and relative peak power per fat free mass (W.kg(-1)FFM) during the 30-second Wingate Test (WT30) on bicycle ergometer (Monark 894E Peak bike, MONARK, Sweden). All WT30s were performed during the hockey season. Overall genotype frequencies were 50.6% GG homozygotes, 40.3% CG heterozygotes, and 9.1% CC homozygotes. We found statistically significant differences in Pmax.kg(-1) and marginally significant differences in Pmax.kg(-1)FFM values in WT30 between carriers and non-carriers for C allele (14.6 ± 0.2 vs. 13.9 ± 0.3 W.kg(-1) and 15.8 ± 0.2 vs. 15.2 ± 0.3 W.kg(-1)FFM, P = 0.036 and 0.12, respectively). Furthermore, Pmax.kg(-1)FFM strongly positively correlated with the body weight only in individuals with GG genotypes (R = 0.55; p<0.001). Our results indicate that PPARA 7C carriers exhibited higher speed strength measures in WT30. We hypothesize that C allele carriers within the cohort of trained individuals may possess a metabolic advantage towards anaerobic metabolism.
authors.affiliation: Department of Physiology and Biochemistry, Faculty of Physical Education and Sport, Charles University in Prague, Prague, Czech Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Petr
authors.forename: Petr
authors.initials: P
authors.lastname: Stastny
authors.affiliation: Department of Sport Games, Faculty of Physical Education and Sport, Charles University in Prague, Prague, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Št'astný
authors.affiliation: Department of Kinanthropology and Humanities, Faculty of Physical Education and Sport, Charles University in Prague, Prague, Czech Republic.
authors.forename: Ondřej
authors.initials: O
authors.lastname: Pecha
authors.affiliation: Department of Physiology and Biochemistry, Faculty of Physical Education and Sport, Charles University in Prague, Prague, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Šteffl
authors.affiliation: Institute of Biology and Medical Genetics, the First Faculty of Medicine, Charles University and the General Teaching Hospital, Prague, Czech Republic.
authors.forename: Ondřej
authors.initials: O
authors.lastname: Šeda
authors.affiliation: Department of Physiology and Biochemistry, Faculty of Physical Education and Sport, Charles University in Prague, Prague, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Kohlíková
mesh_terms: D000293:Adolescent; D000328:Adult; D000693:Anaerobiosis; D054874:Athletic Performance; D001835:Body Weight / Q000235:genetics; D005080:Exercise Test; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007438:Introns / Q000235:genetics*; D008297:Male; D047493:PPAR alpha / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
chemical_list: D047493:PPAR alpha