title: Fluorescent gold nanoclusters based photoelectrochemical sensors for detection of H2O2 and glucose.
abstract: In this work, low-toxicity fluorescent gold nanoclusters (AuNCs) based photoelectrochemical sensors were developed for H2O2 and glucose detection. Herein, the processes used to fabricate the sensors and the photoelectrochemical performances of the sensors under different conditions were presented. Based on the energy band levels of the AuNCs and electron tunneling processes, a detailed photoelectrochemical sensing model was given. The designed sensors were then used for H2O2 and glucose detection without any extra modification of the AuNCs or complex enzyme immobilization. The results demonstrate that the AuNCs allow for H2O2 sensing based on their capacity for both fluorescence and catalysis. Indeed, it was observed that H2O2 was catalyzed by the AuNCs and reduced by photoinduced electrons derived from excited AuNCs. Furthermore, an enhancement in photocurrent amplitude followed the increase in the concentrations of H2O2 and glucose. The effects of the types of ligands surrounding the AuNCs and the applied potential on the output photocurrent were well studied to optimize the measurement conditions. The sensitivity and LOD of MUA-AuNCs at -500 mV were 4.33 nA/mM and 35 μM, respectively. All experimental results indicated that AuNCs could not only serve as a promising photoelectrical material for building the photoelectrochemical biosensors but as catalysts for H2O2 sensing.
authors.affiliation: Department of Microelectronics, Nankai University, Tianjin 300071, China.
authors.forename: Jianxiu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Microelectronics, Nankai University, Tianjin 300071, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Tu
authors.affiliation: Department of Microelectronics, Nankai University, Tianjin 300071, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Microelectronics, Nankai University, Tianjin 300071, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Liu
authors.affiliation: Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, School for Radiological & Interdisciplinary Sciences (RAD-X), Soochow University, Suzhou 215123, China.
authors.forename: Yangyun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, School for Radiological & Interdisciplinary Sciences (RAD-X), Soochow University, Suzhou 215123, China. Electronic address: wangyongac@gmail.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Microelectronics, Nankai University, Tianjin 300071, China. Electronic address: yuezhao@nankai.edu.cn.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yue
mesh_terms: D015374:Biosensing Techniques; D005456:Fluorescent Dyes / Q000737:chemistry; D005947:Glucose / Q000737:chemistry / Q000302:isolation & purification*; D006046:Gold / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000302:isolation & purification*; D053768:Metal Nanoparticles / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D006046:Gold; D006861:Hydrogen Peroxide; D005947:Glucose
keywords: Fluorescent gold nanoclusters; Glucose; H(2)O(2); Photocurrent; Photoelectrochemistry