title: Extracellular polymeric substances production kinetics of 13 sludge isolates using wastewater sludge as raw material and its flocculation potential.
abstract: The kinetics of batch fermentation of 13 extracellular polymeric substances (EPS) producing bacterial strains (9 Bacillus, 2 Serratia and 2 Yersinia) were carried out using sterilized sludge as a raw material. The most of Bacillus (µ(max): 0.11-0.27 h⁻¹), Serratia (µ(max): 0.23-0.27 h⁻¹) and Yersinia (µ(max): 0.18-0.19 h⁻¹) strains had capability to grow and produce EPS (1.36-2.12 g/L) in the sterilized sludge. In general, EPS production was mixed growth associated for all the bacterial strains cultivated independently. Bacillus sp. 7, Serratia sp. 2 and Yersinia sp. 2 produced higher concentration (1.95-2.12 g/L) of EPS than the other remaining bacterial strains. Protein and carbohydrate contents of EPS remained constant during fermentation. Broth EPS (B-EPS) exhibited high kaolin flocculation activity (≥ 75%) in most of the cases except Bacillus sp. 1, Bacillus sp. 5 and Bacillus sp. 9, respectively. In general, high flocculation activities (FAs) (≥ 75%), were attained using 1.31-1.70 mg B-EPS/g kaolin, 0.45-0.97 mg protein/g kaolin and 0.11-0.21 mg carbohydrates/g kaolin. The study suggests that further systematic exploration is required for optimizing the process of EPS production. EPS produced in the sludge can potentially be used for different water and wastewater treatments.
authors.affiliation: a Université du Québec, Institut national de la recherche scientifique, Centre Eau, Terre et Environnement , 490 de la Couronne, Québec ( QC ), Canada G1 K 9A9.
authors.forename: Tanaji
authors.initials: T
authors.lastname: More
authors.affiliation: a Université du Québec, Institut national de la recherche scientifique, Centre Eau, Terre et Environnement , 490 de la Couronne, Québec ( QC ), Canada G1 K 9A9.
authors.forename: Amine
authors.initials: A
authors.lastname: Mahmoudi
authors.affiliation: a Université du Québec, Institut national de la recherche scientifique, Centre Eau, Terre et Environnement , 490 de la Couronne, Québec ( QC ), Canada G1 K 9A9.
authors.forename: Song
authors.initials: S
authors.lastname: Yan
authors.affiliation: a Université du Québec, Institut national de la recherche scientifique, Centre Eau, Terre et Environnement , 490 de la Couronne, Québec ( QC ), Canada G1 K 9A9.
authors.forename: Rajeshwar Dayal
authors.initials: RD
authors.lastname: Tyagi
mesh_terms: D001407:Bacillus / Q000254:growth & development / Q000378:metabolism; D001704:Biopolymers / Q000096:biosynthesis*; D005427:Flocculation; D007616:Kaolin / Q000737:chemistry; D007700:Kinetics; D012705:Serratia / Q000254:growth & development / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*; D015007:Yersinia / Q000254:growth & development / Q000378:metabolism
chemical_list: D001704:Biopolymers; D012722:Sewage; D007616:Kaolin
keywords: extracellular polymeric substances; growth rate; kinetics; production; wastewater sludge