title: Establishment of an enzyme-linked immunosorbent assay and application on determination of ginsenoside Re in human saliva.
abstract: This work describes an immunochemical approach for the quality control of Panax ginseng and a pharmacological study of ginsenoside Re, a major bioactive constituent in P. ginseng, using an enzyme-linked immunosorbent assay. A hybridoma secreting monoclonal antibody against ginsenoside Re was produced by fusing splenocytes immunized with a ginsenoside Re-bovine serum albumin conjugate with the hypoxanthine-aminopterin-thymidine-sensitive mouse myeloma SP2/0 cell line. The method, at an effective measuring range of 7.8-500 ng · mL(-1) of ginsenoside Re, successfully detected ginsenoside Re in Chinese traditional herb prescriptions. The results demonstrate that we generated a novel and reliable assay system for measuring ginsenoside Re in Chinese medicines more efficiently. Futhermore, we determined the ginsenoside Re concentrations in the saliva of six healthy adults after the oral administration of a ginseng capsule to study the pharmacokinetics of ginsenoside Re in human saliva.
authors.affiliation: Center of Scientific Experiment, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China.
authors.forename: Huihua
authors.initials: H
authors.lastname: Qu
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China.
authors.forename: Jiayang
authors.initials: J
authors.lastname: Sai
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Sun
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China.
authors.forename: Yifei
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Chaoyang District, Beijing, China.
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D036145:Ginsenosides / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics / Q000592:standards; D006801:Humans; D006825:Hybridomas; D008516:Medicine, Chinese Traditional / Q000592:standards; D008807:Mice, Inbred BALB C; D005894:Panax / Q000737:chemistry*; D011786:Quality Control; D012463:Saliva / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D036145:Ginsenosides; C049864:ginsenoside Re