title: Positive reinforcing effects of RFamide-related peptide-1 in the rat central nucleus of amygdala.
abstract: The amygdaloid body (AMY) plays an important role in memory, learning and reward-related processes. RFamide-related peptide-1 (RFRP-1) immunoreactive fibers and NPFF1 receptors were identified in the AMY, and previously we verified that neuropeptide RFRP-1 infused into the central nucleus of AMY (CeA) caused food intake decrease. The aim of the present study was to examine the possible rewarding or aversive effects of RFRP-1 in the CeA. In conditioned place preference, test male Wistar rats were microinjected bilaterally with 50 or 100ng RFRP-1 in volume of 0.4μl. In other groups of animals, 20ng NPFF receptor antagonist RF9 was applied or the antagonist was used 15min before 50ng RFRP-1 treatment. Fifty nanograms of RFRP-1 had positive reinforcing properties, while 100ng RFRP-1 had no effect. Prior treatment with NPFF receptor antagonist RF9 could block the rewarding effects of RFRP-1, while the antagonist applied alone did not influence the behavior of rats in place preference paradigm. Our results show that RFRP-1 and NPFF-1 receptors play important roles in the amygdaloid rewarding-reinforcing mechanisms.
authors.affiliation: Institute of Physiology, Pécs University Medical School, Pécs, Hungary; Molecular Neurophysiology Research Group, Pécs University, Szentágothai Research Center, Pécs, Hungary. Electronic address: Laszlo.Lenard@aok.pte.hu.
authors.forename: László
authors.initials: L
authors.lastname: Lénárd
authors.affiliation: Institute of Physiology, Pécs University Medical School, Pécs, Hungary.
authors.forename: Anita
authors.initials: A
authors.lastname: Kovács
authors.affiliation: Institute of Physiology, Pécs University Medical School, Pécs, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Ollmann
authors.affiliation: Institute of Physiology, Pécs University Medical School, Pécs, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Péczely
authors.affiliation: Institute of Physiology, Pécs University Medical School, Pécs, Hungary.
authors.forename: Olga
authors.initials: O
authors.lastname: Zagoracz
authors.affiliation: Institute of Physiology, Pécs University Medical School, Pécs, Hungary.
authors.forename: Rita
authors.initials: R
authors.lastname: Gálosi
authors.affiliation: Institute of Physiology, Pécs University Medical School, Pécs, Hungary.
authors.forename: Kristóf
authors.initials: K
authors.lastname: László
mesh_terms: D000218:Adamantane / Q000031:analogs & derivatives / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D066274:Central Amygdaloid Nucleus / Q000187:drug effects* / Q000502:physiology; D003216:Conditioning, Operant / Q000187:drug effects*; D004151:Dipeptides / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D008297:Male; D008845:Microinjections; D009479:Neuropeptides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012054:Reinforcement, Psychology
chemical_list: D004151:Dipeptides; D009479:Neuropeptides; C416987:RFamide peptide; C508361:adamantylcarbonyl-arginyl-phenylalaninamide; D000218:Adamantane
keywords: Amygdala; NPFF receptor antagonist RF9; Place preference; RFRP-1; Reinforcement