title: Apigenin mediated protection of OGD-evoked neuron-like injury in differentiated PC12 cells.
abstract: Ischemic stroke has been confirmed to cause neuronal injury due to its insufficient supply of glucose and oxygen to brain tissue. Previous research has shown that oxidative stress, a result of excessive accumulation of reactive oxygen species (ROS), relates to pathophysiology of ischemic stroke, and causes oxidative damage to biomolecules, eventually leading to programmed cell death. Meanwhile, apigenin has been shown to exhibit antioxidant, anti-inflammatory, anti-cancer properties and neuroprotective action. Hence, this study was to investigate the potential mechanisms underlying the neural protection of apigenin on oxygen and glucose deprivation/reperfusion (OGD/R) induced neuronal injury in differentiated PC12 cells. Cells were pretreated with apigenin for 6 h, and then subjected to OGD for 12 h followed by reperfusion for 24 h. The results showed that OGD/R significantly decreased cell viability, mitochondrial membrane potential, mRNA levels of antioxidant and detoxifying enzymes and Nrf2 protein expression, while elevated the release of LDH, cell apoptosis, intracellular ROS level, P53 protein expression and upregulated its downstream genes in PC12 cells. However, apigenin effectively inhibited these undesirable changes induced by OGD/R. Our findings demonstrate that this compound attenuates OGD/R induced neuronal injury mainly by virtue of its anti-apoptosis and antioxidative properties via affecting the expression of Nrf2 and P53, and their downstream target gene transcription.
authors.affiliation: The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Huizhen
authors.initials: H
authors.lastname: Guo
authors.forename: Songzhi
authors.initials: S
authors.lastname: Kong
authors.forename: Weimin
authors.initials: W
authors.lastname: Chen
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Dai
authors.forename: Tongxiang
authors.initials: T
authors.lastname: Lin
authors.forename: Jiyan
authors.initials: J
authors.lastname: Su
authors.forename: Shanshan
authors.initials: S
authors.lastname: Li
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Xie
authors.forename: Ziren
authors.initials: Z
authors.lastname: Su
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Lai
mesh_terms: D000818:Animals; D047310:Apigenin / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D047310:Apigenin; D005947:Glucose; D010100:Oxygen