title: Biocytin staining of glia and neurons in brain slices.
abstract: This protocol describes the use of biocytin to visualize and distinguish the morphology of glia and neurons in rat brain slices. Patch pipettes are used to load biocytin into different cell types. The slices are subsequently fixed, stained, and mounted in preparation for imaging.
authors.forename: Jian
authors.initials: J
authors.lastname: Kang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology*; D005260:Female; D066298:In Vitro Techniques; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism; D008297:Male; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C013411:biocytin; D008239:Lysine