title: Nematicidal activity of Paecilomyces lilacinus 6029 cultured on Karanja cake medium.
abstract: Antagonistic fungi parasitize root-knot nematodes through secretion of extracellular hydrolytic enzymes and secondary metabolites. In present study, in vitro bioassay showed that Paecilomyces lilacinus 6029 culture filtrate from Karanja cake medium killed 100% Meloidogyne incognita larvae while only 78.28% mortality was recorded by Czapeck-Dox filtrate within 12 h of exposure. The filtrate, irrespective of culture medium, was found to be more nematotoxic when incubated for 15 days. Fourier Transform infrared spectroscopy predicted the presence of phenolic and alcoholic compounds in the filtrate. Furthermore, the active metabolites in fungal filtrate were partially characterized. pH stability test revealed that nematotoxicity of the filtrate appeared at all range of pH with low pH filtrate possessing more toxicity against M. incognita. Interestingly, buffers of same pH value did not show any nematicidal effect. No significant difference in nematicidal activity was observed between boiled (98.2% mortality) and unboiled culture filtrate (100% mortality). Ethyl acetate and lyophilized aqueous extracts produced higher nematicidal activity than a hexane extract indicating polar nature of active compounds produced by P. lilacinus 6029.
authors.affiliation: Centre for Rural Development & Technology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Centre for Rural Development & Technology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India. Electronic address: questabhi@gmail.com.
authors.forename: Satyawati
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Centre for Biomedical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.
authors.forename: Manu
authors.initials: M
authors.lastname: Dalela
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry*; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D009348:Nematoda / Q000187:drug effects* / Q000502:physiology*; D010143:Paecilomyces / Q000254:growth & development* / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D016019:Survival Analysis
chemical_list: D000871:Anthelmintics; D003470:Culture Media
keywords: Culture filtrate; Karanja cake; Meloidogyne incognita; Nematicidal; Paecilomyces lilacinus