title: Adenovirus E1A targets the DREF nuclear factor to regulate virus gene expression, DNA replication, and growth.
abstract: The adenovirus E1A gene is the first gene expressed upon viral infection. E1A remodels the cellular environment to maximize permissivity for viral replication. E1A is also the major transactivator of viral early gene expression and a coregulator of a large number of cellular genes. E1A carries out its functions predominantly by binding to cellular regulatory proteins and altering their activities. The unstructured nature of E1A enables it to bind to a large variety of cellular proteins and form new molecular complexes with novel functions. The C terminus of E1A is the least-characterized region of the protein, with few known binding partners. Here we report the identification of cellular factor DREF (ZBED1) as a novel and direct binding partner of E1A. Our studies identify a dual role for DREF in the viral life cycle. DREF contributes to activation of gene expression from all viral promoters early in infection. Unexpectedly, it also functions as a growth restriction factor for adenovirus as knockdown of DREF enhances virus growth and increases viral genome copy number late in the infection. We also identify DREF as a component of viral replication centers. E1A affects the subcellular distribution of DREF within PML bodies and enhances DREF SUMOylation. Our findings identify DREF as a novel E1A C terminus binding partner and provide evidence supporting a role for DREF in viral replication.
  
  
  OBJECTIVE
  This work identifies the putative transcription factor DREF as a new target of the E1A oncoproteins of human adenovirus. DREF was found to primarily localize with PML nuclear bodies in uninfected cells and to relocalize into virus replication centers during infection. DREF was also found to be SUMOylated, and this was enhanced in the presence of E1A. Knockdown of DREF reduced the levels of viral transcripts detected at 20 h, but not at 40 h, postinfection, increased overall virus yield, and enhanced viral DNA replication. DREF was also found to localize to viral promoters during infection together with E1A. These results suggest that DREF contributes to activation of viral gene expression. However, like several other PML-associated proteins, DREF also appears to function as a growth restriction factor for adenovirus infection.
authors.affiliation: Department of Microbiology, University of Manitoba, Winnipeg, MB, Canada.
authors.forename: Sandi
authors.initials: S
authors.lastname: Radko
authors.affiliation: Department of Microbiology, University of Manitoba, Winnipeg, MB, Canada.
authors.forename: Maria
authors.initials: M
authors.lastname: Koleva
authors.affiliation: Department of Microbiology and Immunology, Western University, London, ON, Canada.
authors.forename: Kris M D
authors.initials: KM
authors.lastname: James
authors.affiliation: Department of Microbiology, University of Manitoba, Winnipeg, MB, Canada.
authors.forename: Richard
authors.initials: R
authors.lastname: Jung
authors.affiliation: Department of Microbiology and Immunology, Western University, London, ON, Canada Department of Oncology, Western University, London, ON, Canada.
authors.forename: Joe S
authors.initials: JS
authors.lastname: Mymryk
authors.affiliation: Department of Microbiology, University of Manitoba, Winnipeg, MB, Canada peter.pelka@umanitoba.ca.
authors.forename: Peter
authors.initials: P
authors.lastname: Pelka
mesh_terms: D017500:Adenovirus E1A Proteins / Q000378:metabolism*; D000260:Adenoviruses, Human / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D002460:Cell Line; D004261:DNA Replication; D015967:Gene Expression Regulation, Viral; D054884:Host-Pathogen Interactions; D006801:Humans; D011485:Protein Binding; D025941:Protein Interaction Mapping; D014157:Transcription Factors / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D017500:Adenovirus E1A Proteins; D014157:Transcription Factors; C518505:ZBED1 protein, human