title: Gabapentin inhibits central sensitization during migraine.
abstract: Peripheral and central sensitizations are phenomena that occur during migraine. The role of pentin, a migraine preventive drug, on central sensitization remains unclear. In this study, a rat model of migraine was established by electrical stimulation of the trigeminal ganglion, and the an-imals were given intragastric gabapentin. Changes in amino acid content in the cerebrospinal fluid and protein kinase C membrane translocation in the spinal trigeminal nucleus were examined to clarify the mechanisms underlying the efficacy of gabapentin in the treatment of central sensitization during migraine. Electrophysiology, liquid chromatography-mass spectrometry and western blot analysis results revealed that gabapentin reduces neuronal excitability in the spinal nucleus in the trigeminal nerve, decreases excitatory amino acid content and inhibits the activation of protein ki-nase C. This provides evidence that excitatory amino acids and protein kinase C are involved in the formation and maintenance of central sensitization during migraine. Gabapentin inhibits migraine by reducing excitatory amino acid content in the cerebrospinal fluid and inhibiting protein kinase C ac-tivation.
authors.affiliation: Institute of Hypoxia Medicine, Xuanwu Hospital, Capital Medical University, Beijing 100053, China ; Department of Neurology, Affiliated Hospital of Taishan Medical University, Tai'an 271000, Shandong Province, China.
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Biomedical Research Center, Baotou Medical College, Baotou 014060, Inner Mongolia Autonomous Region, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Shao
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital of Baotou Medical College, Baotou 014010, Inner Mongolia Autonomous Region, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Institute of Hypoxia Medicine, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.
authors.forename: Sijie
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Neurology, Affiliated Hospital of Taishan Medical University, Tai'an 271000, Shandong Province, China.
authors.forename: Jingzhong
authors.initials: J
authors.lastname: Niu
authors.affiliation: Department of Neurology, Affiliated Hospital of Taishan Medical University, Tai'an 271000, Shandong Province, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Hu
authors.affiliation: Department of Neurology, Affiliated Hospital of Taishan Medical University, Tai'an 271000, Shandong Province, China.
authors.forename: Mingfeng
authors.initials: M
authors.lastname: Yang
authors.affiliation: Institute of Hypoxia Medicine, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.
authors.forename: Xunming
authors.initials: X
authors.lastname: Ji
keywords: calcitonin gene-related peptide; excitatory amino acid; gamma-aminobutyric acid; glutamic acid; grants-supported paper; ionic excitatory amino acid receptors; migraine; neural regeneration; neurons; neuroregeneration; nitric oxide synthase; nuclei of trigeminal nerve; protein kinase C