title: Neutrophil gelatinase-associated lipocalin (NGAL) and insulin-like growth factor (IGF)-1 association with a Mannheimia haemolytica infection in sheep.
abstract: This study was aimed at mapping the tissue distribution of some inflammatory parameters associated with a Mannheimia haemolytica (M. haemolytica) infection in sheep. The M. haemolytica was isolated and characterized from the affected lungs of slaughtered animals. Cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-10, insulin-like growth factor (IGF)-1, as well as the acute-phase protein, neutrophil gelatinase-associated lipocalin (NGAL), were identified in the lung tissues, the serum, and the lymph nodes of M. haemolytica infected sheep, by enzyme-linked immunosorbent assay (ELISA). NGAL and IGF-1 pointed to an innate immune response, and epithelial cell repairing, respectively. The adaptive immune response was identified through the type of cytokines present in the affected sheep, as TNF-α represents the pro-inflammatory cytokines, and IL-10 represents the anti-inflammatory cytokines. M. haemolytica isolates were confirmed by polymerase chain reaction (PCR) and DNA sequences. There was a significant difference in the concentrations of NGAL, IGF-1, TNF-α, and IL-10, as observed in the affected sheep when compared to the healthy sheep. This study, for the first time, closely describes the distribution of some key and new inflammatory parameters in the tissue homogenate of affected lungs.
authors.affiliation: Department of Basic Veterinary Medical Science, Faculty of Veterinary Medicine, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan. Electronic address: tarazi@just.edu.jo.
authors.forename: Yaser H
authors.initials: YH
authors.lastname: Tarazi
authors.affiliation: Department of Basic Veterinary Medical Science, Faculty of Veterinary Medicine, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan. Electronic address: mskn@just.edu.jo.
authors.forename: Mohammad S
authors.initials: MS
authors.lastname: Khalifeh
authors.affiliation: Department of Basic Veterinary Medical Science, Faculty of Veterinary Medicine, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan.
authors.forename: Mohammad M
authors.initials: MM
authors.lastname: Abu Al-Kebash
authors.affiliation: Department of Animal Protection and Prevention, Faculty of Agriculture, P.O. Box 301, Irbid 26150, Jordan.
authors.forename: Mohammad H
authors.initials: MH
authors.lastname: Gharaibeh
mesh_terms: D000209:Acute-Phase Proteins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D007249:Inflammation / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D054834:Lipocalins / Q000235:genetics / Q000378:metabolism*; D008168:Lung / Q000473:pathology; D008198:Lymph Nodes / Q000378:metabolism; D008297:Male; D016978:Mannheimia haemolytica; D012766:Pasteurellosis, Pneumonic / Q000378:metabolism / Q000382:microbiology; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D012756:Sheep; D012757:Sheep Diseases / Q000378:metabolism / Q000382:microbiology*
chemical_list: D000209:Acute-Phase Proteins; D054834:Lipocalins; D011518:Proto-Oncogene Proteins; D007334:Insulin-Like Growth Factor I
keywords: IGF-1; IL-10; Mannheimia haemolytica; NGAL; Sheep; TNF-α