title: Virus-based surface patterning of biological molecules, probes, and inorganic materials.
abstract: An essential requirement for continued technological advancement in many areas of biology, physics, chemistry, and materials science is the growing need to generate custom patterned materials. Building from recent achievements in the site-specific modification of virus for covalent surface tethering, we show in this work that stable 2D virus patterns can be generated in custom geometries over large area glass surfaces to yield templates of biological, biochemical, and inorganic materials in high density. As a nanomaterial building block, filamentous viruses have been extensively used in recent years to produce materials with interesting properties, owing to their ease of genetic and chemical modification. By utilizing un-natural amino acids generated at specific locations on the filamentous fd bacteriophage protein coat, surface immobilization is carried out on APTES patterned glass resulting in precise geometries of covalently linked virus material. This technique facilitated the surface display of a high density of virus that were labeled with biomolecules, fluorescent probes, and gold nanoparticles, thereby opening the possibility of integrating virus as functional components for surface engineering.
authors.affiliation: Department of Chemical Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea; Institute of Nanoscience and Technology, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea.
authors.forename: Suji
authors.initials: S
authors.lastname: Ahn
authors.affiliation: Department of Chemical Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea; Institute of Nanoscience and Technology, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea.
authors.forename: Seongho
authors.initials: S
authors.lastname: Jeon
authors.affiliation: Department of Chemical Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea; Institute of Nanoscience and Technology, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea.
authors.forename: Eun-A
authors.initials: EA
authors.lastname: Kwak
authors.affiliation: Department of Chemical Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea; Institute of Nanoscience and Technology, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea.
authors.forename: Jong-Man
authors.initials: JM
authors.lastname: Kim
authors.affiliation: Department of Chemical Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea; Institute of Nanoscience and Technology, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea. Electronic address: justynj@hanyang.ac.kr.
authors.forename: Justyn
authors.initials: J
authors.lastname: Jaworski
mesh_terms: D007287:Inorganic Chemicals / Q000737:chemistry*; D015335:Molecular Probes; D013499:Surface Properties; D014780:Viruses / Q000737:chemistry*
chemical_list: D007287:Inorganic Chemicals; D015335:Molecular Probes
keywords: Covalent surface modification; Filamentous virus; Fluorescent probes; Gold nanoparticles; Un-natural amino acids