title: Development of a calcium phosphate nanocomposite for fast fluorogenic detection of bacteria.
abstract: Current procedures for the detection and identification of bacterial infections are laborious, time-consuming, and require a high workload and well-equipped laboratories. Therefore the work presented herein developed a simple, fast, and low cost method for bacterial detection based on hydroxyapatite nanoparticles with a nutritive mixture and the fluorogenic substrate. Calcium phosphate ceramic nanoparticles were characterized and integrated with a nutritive mixture for the early detection of bacteria by visual as well as fluorescence spectroscopy techniques. The composite was obtained by combining calcium phosphate nanoparticles (Ca:P ratio, 1.33:1) with a nutritive mixture of protein hydrolysates and carbon sources, which promote fast bacterial multiplication, and the fluorogenic substrate 4-methylumbellipheryl-β-D-glucuronide (MUG). The composite had an average particle size of 173.2 nm and did not show antibacterial activity against Gram-negative or Gram-positive bacteria. After an Escherichia coli suspension was in contact with the composite for 60-90 min, fluorescence detected under UV light or by fluorescence spectrophotometer indicated the presence of bacteria. Intense fluorescence was observed after incubation for a maximum of 90 min. Thus, this calcium phosphate nanocomposite system may be useful as a model for the development of other nanoparticle composites for detection of early bacterial adhesion.
authors.affiliation: Centro Nacional de Biopreparados, Carretera a Beltrán Km 1 1/2, Bejucal, Mayabeque, Apartado 6048, Cuba. claudio@biocen.cu.
authors.forename: Claudio R
authors.initials: CR
authors.lastname: Martínez
authors.affiliation: Centro Nacional de Biopreparados, Carretera a Beltrán Km 1 1/2, Bejucal, Mayabeque, Apartado 6048, Cuba. lobaina@biocen.cu.
authors.forename: Tamara L
authors.initials: TL
authors.lastname: Rodríguez
authors.affiliation: Centro Nacional de Biopreparados, Carretera a Beltrán Km 1 1/2, Bejucal, Mayabeque, Apartado 6048, Cuba. raisa@biocen.cu.
authors.forename: Raisa
authors.initials: R
authors.lastname: Zhurbenko
authors.affiliation: Centro Nacional de Biopreparados, Carretera a Beltrán Km 1 1/2, Bejucal, Mayabeque, Apartado 6048, Cuba. ivonne.alfonso@biocen.cu.
authors.forename: Ivonne A
authors.initials: IA
authors.lastname: Valdés
authors.affiliation: Restorative Dentistry Department, Faculty of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, MG, Brazil. savio.morato@yahoo.com.br.
authors.forename: Sávio M L
authors.initials: SM
authors.lastname: Gontijo
authors.affiliation: Chemistry Department, ICEx, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, MG, Brazil. alinnedamasia@gmail.com.
authors.forename: Alinne D M
authors.initials: AD
authors.lastname: Gomes
authors.affiliation: Chemistry Department, ICEx, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, MG, Brazil. diego.fersuar@gmail.com.
authors.forename: Diego F
authors.initials: DF
authors.lastname: Suarez
authors.affiliation: Chemistry Department, ICEx, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, MG, Brazil. sinisterra@ufmg.br.
authors.forename: Rubén D
authors.initials: RD
authors.lastname: Sinisterra
authors.affiliation: Restorative Dentistry Department, Faculty of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, MG, Brazil. mecortes@ufmg.br.
authors.forename: Maria E
authors.initials: ME
authors.lastname: Cortés
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001424:Bacterial Infections / Q000175:diagnosis; D002516:Ceramics / Q000737:chemistry*; D013293:Enterococcus faecalis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry* / Q000494:pharmacology; D057230:Limit of Detection; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013211:Staphylococcus aureus / Q000187:drug effects; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D006882:Hydroxyapatites