title: Development of SSR markers for the tropical alpine tree species Leptospermum recurvum (Myrtaceae) on Mount Kinabalu in Borneo.
abstract: OBJECTIVE
  Nuclear microsatellite (simple sequence repeat [SSR]) markers were developed for the woody species Leptospermum recurvum found on Mount Kinabalu, Borneo, to facilitate investigation of the genetic structure and patterns of gene flow in relation to leaf phenotypic polymorphisms. •
  
  
  RESULTS
  Eleven primer pairs were developed using the compound SSR marker technique. Ten of the 11 loci were polymorphic and their expected heterozygosity ranged from 0.04 to 0.83. Neither linkage disequilibrium nor departure from Hardy-Weinberg equilibrium were detected. All primer pairs also amplified the SSR loci of L. polygalifolium. •
  
  
  CONCLUSIONS
  These findings suggest the utility of these primers for investigating genetic structure and gene flow in L. recurvum and indicate their applicability to another species of Leptospermum.
authors.affiliation: Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Soichi
authors.initials: S
authors.lastname: Ando
authors.affiliation: Faculty of Symbiotic Systems Science, Fukushima University, 1 Kanayagawa, Fukushima 960-1296, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Kaneko
authors.affiliation: Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Isagi
authors.affiliation: Sabah Parks, P.O. Box 10626, 88806 Kota Kinabalu, Malaysia.
authors.forename: Rimi
authors.initials: R
authors.lastname: Repin
authors.affiliation: Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Kanehiro
authors.initials: K
authors.lastname: Kitayama
keywords: Leptospermum recurvum; Mount Kinabalu; Myrtaceae; simple sequence repeat (SSR) markers