title: Stacking and analysis of melamine in milk products with acetonitrile-salt stacking technique in capillary electrophoresis.
abstract: Melamine was measured in real milk products with capillary electrophoresis (CE) based on acetonitrile-salt stacking (ASS) method. Real milk samples were deproteinized with acetonitrile at a final concentration of 60% (v/v) and then injected hydrodynamically at 50 mBar for 40.0 s. The optimized buffer contains 80.0 mmol/L pH 2.8 phosphates. Melamine could be detected within 20.0 min at +10 kV with a low limit of detection (LOD) of 0.03 μmol/L. Satisfactory reproducibility (inter- and intraday RSD% both for migration time and peak area was lower than 5.0%) and a wide linearity range of 0.05 μmol/L ~ 10.0 μmol/L were achieved. The proposed method was suitable for routine assay of MEL in real milk samples that was subjected to a simple treatment step.
authors.affiliation: Institute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Yu
authors.identifier: 0000-0002-2241-1519
authors.initials: Y
authors.lastname: Kong
authors.affiliation: Department of Criminal and Inspectoral, Public Security Bureau of Han Zhong City, Shaanxi, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Wei
authors.affiliation: Institute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Zhanwu
authors.initials: Z
authors.lastname: Hou
authors.affiliation: Institute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Zilong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Institute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Yu
authors.affiliation: Institute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Yongxi
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Institute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Meili
authors.initials: M
authors.lastname: Gao