title: A new triterpene and protective effect of Periploca somaliensis Browicz fruits against CCl₄-induced injury on human hepatoma cell line (Huh7).
abstract: The potential hepatoprotective effect of the methanolic extract of Periploca somaliensis Browicz fruits, its different fractions (n-hexane, chloroform and n-butanol) and the major isolated compound ursolic acid was evaluated using the human hepatoma cell line (Huh7) based on the changes in the activity of aspartate aminotransferase, alanine transaminase, glutathione and superoxide dismutase. Each sample was tested at three different concentrations (1000, 100 and 10 μg/mL). All tested samples exhibited a potent concentration-independent cytoprotective effect relative to silymarin as a reference standard. Chromatographic fractionation of the chloroform-soluble fraction of the methanol extract of P. somaliensis Browicz fruits afforded two known triterpenes, namely ursolic acid, and 11α,12α-epoxy-3β-hydroxy-olean-13β,28-olide, and a newly discovered one, namely 3β-hydroxy-urs-11-en-13β,28-olide. The structures of the isolated compounds were elucidated by the analysis of 1D and 2D NMR spectral data.
authors.affiliation: a Department of Pharmacognosy , Faculty of Pharmacy, Cairo University , 11562 Cairo , Egypt.
authors.forename: Azza R
authors.initials: AR
authors.lastname: Abdel-Monem
authors.forename: Zeinab A
authors.initials: ZA
authors.lastname: Kandil
authors.forename: Ashraf B
authors.initials: AB
authors.lastname: Abdel-Naim
authors.forename: Essam
authors.initials: E
authors.lastname: Abdel-Sattar
mesh_terms: D002251:Carbon Tetrachloride; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor / Q000187:drug effects; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology; D015394:Molecular Structure; D031109:Periploca / Q000737:chemistry*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D000097245:Ursolic Acid
chemical_list: D002251:Carbon Tetrachloride; D014315:Triterpenes; D000097245:Ursolic Acid; C000597093:3beta-hydroxy-urs-11-en-13beta,28-olide
keywords: Huh7 cell line; Periploca somaliensis Browicz; hepatoprotective; triterpenes