title: The effects of di(2-ethylhexyl) phthalate and/or selenium on trace element levels in different organs of rats.
abstract: Di(2-ethylhexyl)phthalate (DEHP), a widely used plasticizer for synthetic polymers, is known to have endocrine disruptive potential, reproductive toxicity, and induces hepatic carcinogenesis in rodents. Selenium (Se) is a component of several selenoenzymes which are essential for cellular antioxidant defense and for the functions of mammalian reproductive system. The present study was designed to investigate the effects of DEHP exposure on trace element distribution in liver, testis, and kidney tissues and plasma of Se-deficient and Se-supplemented rats. Se deficiency was produced by feeding 3-week old Sprague-Dawley rats with ≤0.05mg Se/kg diet for 5 weeks, and supplementation group were on 1mg Se/kg diet. DEHP treated groups received 1000mg/kg dose by gavage during the last 10 days of feeding period. Se, zinc (Zn), copper (Cu), iron (Fe) and manganese (Mn) levels were measured by inductively coupled plasma mass spectrometry (ICP-MS). Se supplementation caused significant increases in hepatic, renal, and testicular Se levels. With DEHP exposure, plasma Se and Zn, kidney Se, Cu and Mn levels were significantly decreased. Besides, liver Fe decreased markedly in all the DEHP-treated groups. Liver and kidney Mn levels decreased significantly in DEHP/SeD group compared to both DEHP and SeD groups. These results showed the potential of DEHP exposure and/or different Se status to modify the distribution pattern of essential trace elements in various tissues, the importance of which needs to be further evaluated.
authors.affiliation: Hacettepe University, Faculty of Pharmacy, Department of Toxicology, Sihhiye, Ankara, Turkey.
authors.forename: Pinar
authors.initials: P
authors.lastname: Erkekoglu
authors.affiliation: Inserm U1055, Laboratoire de Bioénergétique Fondamentale et Appliquée (LBFA), Grenoble F-38000, France; CHU Grenoble, Département de Biochimie, Toxicologie et Pharmacologie, Grenoble F-38000, France.
authors.forename: Josiane
authors.initials: J
authors.lastname: Arnaud
authors.affiliation: CEA Grenoble, INAC/SCIB/LAN, 17 Rue des Martyrs, 38054 Grenoble Cedex 9, France.
authors.forename: Walid
authors.initials: W
authors.lastname: Rachidi
authors.affiliation: Hacettepe University, Faculty of Pharmacy, Department of Toxicology, Sihhiye, Ankara, Turkey. Electronic address: belmagiray@yahoo.fr.
authors.forename: Belma
authors.initials: B
authors.lastname: Kocer-Gumusel
authors.affiliation: CEA Grenoble, INAC/SCIB/LAN, 17 Rue des Martyrs, 38054 Grenoble Cedex 9, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Favier
authors.affiliation: Hacettepe University, Faculty of Pharmacy, Department of Toxicology, Sihhiye, Ankara, Turkey. Electronic address: fhincal@yahoo.com.
authors.forename: Filiz
authors.initials: F
authors.lastname: Hincal
mesh_terms: D000818:Animals; D004051:Diethylhexyl Phthalate / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009928:Organ Specificity / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000494:pharmacology*; D013737:Testis / Q000187:drug effects / Q000378:metabolism; D014018:Tissue Distribution / Q000187:drug effects; D014131:Trace Elements / Q000097:blood / Q000378:metabolism*
chemical_list: D014131:Trace Elements; D004051:Diethylhexyl Phthalate; D012643:Selenium
keywords: Di(ethylhexyl) phthalate (DEHP); Selenium; Selenium deficiency; Selenium supplementation; Trace element