title: Human adipose tissue-derived mesenchymal stem cells inhibit T-cell lymphoma growth in vitro and in vivo.
abstract: OBJECTIVE
  Human mesenchymal stem cells (hMSCs) are thought to be one of the most reliable stem cell sources for a variety of cell therapies. This study investigated the anti-tumor effect of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) on EL4 murine T-cell lymphoma in vitro and in vivo.
  
  
  METHODS
  The growth-inhibitory effect of hAT-MSCs on EL4 tumor cells was evaluated using a WST-1 cell proliferation assay. Cell-cycle arrest and apoptosis were investigated by flow cytometry and western blot. To evaluate an anti-tumor effect of hAT-MSCs on T-cell lymphoma in vivo, CM-DiI-labeled hAT-MSCs were circumtumorally injected in tumor-bearing nude mice, and tumor size was measured.
  
  
  RESULTS
  hAT-MSCs inhibited T-cell lymphoma growth by altering cell-cycle progression and inducing apoptosis in vitro. hAT-MSCs inhibited tumor growth in tumor-bearing nude mice and prolonged survival time. Immunofluorescence analysis showed that hAT-MSCs migrated to tumor sites.
  
  
  CONCLUSIONS
  hAT-MSCs suppress the growth of T-cell lymphoma, suggesting a therapeutic option for T-cell lymphoma.
authors.affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jin-Ok
authors.initials: JO
authors.lastname: Ahn
authors.affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Ji-Sang
authors.initials: JS
authors.lastname: Chae
authors.affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Ye-Rin
authors.initials: YR
authors.lastname: Coh
authors.affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Woo-Sung
authors.initials: WS
authors.lastname: Jung
authors.affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Hee-Woo
authors.initials: HW
authors.lastname: Lee
authors.affiliation: Stem Cell Research Center, K-STEMCELL Co., Ltd, Seoul, Republic of Korea.
authors.forename: Il-Seob
authors.initials: IS
authors.lastname: Shin
authors.affiliation: Stem Cell Research Center, K-STEMCELL Co., Ltd, Seoul, Republic of Korea.
authors.forename: Sung-Keun
authors.initials: SK
authors.lastname: Kang
authors.affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea hyyoun@snu.ac.kr.
authors.forename: Hwa-Young
authors.initials: HY
authors.lastname: Youn
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D017209:Apoptosis; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018920:Coculture Techniques; D004195:Disease Models, Animal; D006801:Humans; D016399:Lymphoma, T-Cell / Q000378:metabolism* / Q000401:mortality / Q000473:pathology*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D047368:Tumor Burden
keywords: Mesenchymal stem cell; T-cell lymphoma; cytotherapy; growth inhibition