title: Stability of iron-quercetin complexes in synthetic wine under in vitro digestion conditions.
abstract: Wine is a dietary source of polyphenolic compounds with reported health benefits when moderately consumed. Several of these compounds can associate with metals forming complexes. Therefore, this work was conducted to reach a better understanding of the nature and chemical stability of wine-derived Fe(3+)-quercetin complexes in a digestion model. The stability of the complexes in a synthetic (simulated) wine was studied before and after in vitro gastric and intestinal digestions by high-performance liquid chromatography (HPLC) with UV-Vis detection. Metal determination was performed by atomic absorption spectrometry (ETAAS) to evaluate possible dissociation of complexes. During HPLC analysis all peaks eluted from the chromatographic column were collected, acidified, and analyzed by ETAAS. The results showed that complexes remain substantially stable after gastric digestion conditions, with recoveries of 84% to 90%. Although metal complexes were partially degraded during intestinal digestion, 41% to 45% of the Fe(3+)-quercetin complexes was recovered.
  
  
  CONCLUSIONS
  This work reveals the chemical stability of Fe3+–quercetin complexes in synthetic wines after an in vitro gastrointestinal digestion. The knowledge of this process would be useful to understand the bioavailability of these compounds.
authors.affiliation: Laboratory of Analytical Chemistry for Research and Development (QUIANID), Facultad de Ciencias Exactas y Naturales, Univ. Nacional de Cuyo, Padre J. Contreras 1300, Mendoza, 5500, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: Leticia B
authors.initials: LB
authors.lastname: Escudero
authors.forename: Cecilia M
authors.initials: CM
authors.lastname: Fusari
authors.forename: Jorgelina C
authors.initials: JC
authors.lastname: Altamirano
authors.forename: Alejandra B
authors.initials: AB
authors.lastname: Camargo
authors.forename: Rodolfo G
authors.initials: RG
authors.lastname: Wuilloud
mesh_terms: D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004063:Digestion / Q000502:physiology; D006801:Humans; D007501:Iron / Q000737:chemistry*; D008954:Models, Biological; D011794:Quercetin / Q000737:chemistry*; D013054:Spectrophotometry, Atomic; D014920:Wine / Q000032:analysis*
chemical_list: D011794:Quercetin; D007501:Iron
keywords: gastric and pancreatic digestion; iron; polyphenols; quercetin; wine