title: Development of microsatellite loci in Artocarpus altilis (Moraceae) and cross-amplification in congeneric species.
abstract: OBJECTIVE
  Microsatellite loci were isolated and characterized from enriched genomic libraries of Artocarpus altilis (breadfruit) and tested in four Artocarpus species and one hybrid. The microsatellite markers provide new tools for further studies in Artocarpus. •
  
  
  RESULTS
  A total of 25 microsatellite loci were evaluated across four Artocarpus species and one hybrid. Twenty-one microsatellite loci were evaluated on A. altilis (241), A. camansi (34), A. mariannensis (15), and A. altilis × mariannensis (64) samples. Nine of those loci plus four additional loci were evaluated on A. heterophyllus (jackfruit, 426) samples. All loci are polymorphic for at least one species. The average number of alleles ranges from two to nine within taxa. •
  
  
  CONCLUSIONS
  These microsatellite primers will facilitate further studies on the genetic structure and evolutionary and domestication history of Artocarpus species. They will aid in cultivar identification and establishing germplasm conservation strategies for breadfruit and jackfruit.
authors.affiliation: Department of Plant Science, Chicago Botanic Garden, Glencoe, Illinois 60022 USA ; Plant Biology and Conservation, Northwestern University, Evanston, Illinois 60208 USA.
authors.forename: Colby
authors.initials: C
authors.lastname: Witherup
authors.affiliation: Breadfruit Institute, National Tropical Botanical Garden, Kalaheo, Hawaii 96741 USA.
authors.forename: Diane
authors.initials: D
authors.lastname: Ragone
authors.affiliation: Plant Biology and Conservation, Northwestern University, Evanston, Illinois 60208 USA.
authors.forename: Tyr
authors.initials: T
authors.lastname: Wiesner-Hanks
authors.affiliation: USDA Tropical Agriculture Research Station, Mayagüez, Puerto Rico 00680.
authors.forename: Brian
authors.initials: B
authors.lastname: Irish
authors.affiliation: USDA, ARS, Genomics and Bioinformatics Research Unit, Stoneville, Mississippi 38776-0350 USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Scheffler
authors.affiliation: USDA, ARS, Genomics and Bioinformatics Research Unit, Stoneville, Mississippi 38776-0350 USA.
authors.forename: Sheron
authors.initials: S
authors.lastname: Simpson
authors.affiliation: USDA Tropical Plant Genetic Resources and Disease Research, Pacific Basin Agricultural Research Center (PBARC), Hilo, Hawaii 96720 USA.
authors.forename: Francis
authors.initials: F
authors.lastname: Zee
authors.affiliation: Plant Biology, University of Rajshahi, Rajshahi, Bangladesh.
authors.forename: M Iqbal
authors.initials: MI
authors.lastname: Zuberi
authors.affiliation: Department of Plant Science, Chicago Botanic Garden, Glencoe, Illinois 60022 USA ; Plant Biology and Conservation, Northwestern University, Evanston, Illinois 60208 USA.
authors.forename: Nyree J C
authors.initials: NJ
authors.lastname: Zerega
keywords: Artocarpus altilis; Artocarpus camansi; Artocarpus heterophyllus; Artocarpus mariannensis; Moraceae; breadfruit; jackfruit