title: Development of new optical imaging systems of oxygen metabolism and simultaneous measurement in hemodynamic changes using awake mice.
abstract: BACKGROUND
  PET allows the measurement of CBF, CBV and CMRO2 in human and plays an important role in the diagnosis of pathologic conditions and clinical research. On the other hand, in animal studies, there is no optical imaging system for evaluating changes in CBF and CBV, and oxygen metabolism, from the same brain area under awake condition.
  
  
  METHODS
  In the present study, we developed a simultaneous measurement system of LSI and IOSI, which was verified by LDF. Moreover, to evaluate oxygen metabolism, FAI was performed from the same brain area as LSI and IOSI measurements.
  
  
  RESULTS
  The change in CBF according to LSI was correlated with that by LDF. Similarly, the change in CBV obtained by IOSI was also correlated with RBC concentration change measured by LDF. The change in oxygen metabolism by FAI was associated with that in CBF obtained by LSI, although the change in CBF was greater than that in oxygen metabolism.
  
  
  CONCLUSIONS
  We revealed that the relationship between oxygen metabolism and CBF as measured by our system was in good agreement with the relationship between CMRO2 and CBF in human PET studies.
  
  
  CONCLUSIONS
  Our measurement system of CBF, CBV and oxygen metabolism is not only useful for studying neurovascular coupling, but also easily corroborates human PET studies.
authors.affiliation: Department of Biophysics, Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Chiba 263-8555, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takuwa
authors.affiliation: Department of Biophysics, Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Chiba 263-8555, Japan; Division of Thermo-Biosystem Relations, United Graduate School of Agricultural Science, Iwate University, 4-3-5 Ueda, Morioka 020-8551, Japan; Department of Chemistry and Bioengineering, Faculty of Engineering, Iwate University, 4-3-5 Ueda, Morioka 020-8551, Japan. Electronic address: matsuura@iwate-u.ac.jp.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Matsuura
authors.affiliation: Department of Biophysics, Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Chiba 263-8555, Japan; Division of Thermo-Biosystem Relations, United Graduate School of Agricultural Science, Iwate University, 4-3-5 Ueda, Morioka 020-8551, Japan.
authors.forename: Asuka
authors.initials: A
authors.lastname: Nishino
authors.affiliation: Department of Chemistry and Bioengineering, Faculty of Engineering, Iwate University, 4-3-5 Ueda, Morioka 020-8551, Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Sakata
authors.affiliation: Department of Biophysics, Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Chiba 263-8555, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Tajima
authors.affiliation: Department of Biophysics, Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Chiba 263-8555, Japan; Advanced Clinical Research Center, Fukushima Global Medical Science Center, Fukushima Medical University, 1 Hikariga-oka, Fukushima 960-1295, Japan. Electronic address: hito@nirs.go.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ito
mesh_terms: D000818:Animals; D001810:Blood Volume / Q000502:physiology; D001921:Brain / Q000378:metabolism*; D002560:Cerebrovascular Circulation / Q000502:physiology; D006439:Hemodynamics / Q000502:physiology*; D017078:Laser-Doppler Flowmetry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D059906:Neuroimaging / Q000295:instrumentation / Q000379:methods*; D055095:Optics and Photonics / Q000379:methods*; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption; D014851:Wakefulness
chemical_list: D010100:Oxygen
keywords: Cerebral blood flow; Cerebral blood volume; Flavoprotein autofluorescence imaging; Intrinsic optical signal imaging; Laser speckle imaging; Oxygen metabolism