title: Thin-layer chromatography combined with diode laser thermal vaporization inductively coupled plasma mass spectrometry.
abstract: Here we present a novel coupling of thin-layer chromatography (TLC) to diode laser thermal vaporization inductively coupled plasma mass spectrometry (DLTV ICP MS). DLTV is a new technique of aerosol generation which uses a diode laser to induce pyrolysis of a substrate. In this case the cellulose stationary phase on aluminum-backed TLC sheets overprinted with black ink to absorb laser light. The experimental arrangement relies on economic instrumentation: an 808-nm 1.2-W continuous-wave infrared diode laser attached to a syringe pump serving as the movable stage. Using a glass tubular cell, the entire length of a TLC separation channel is scanned. The 8-cm long lanes were scanned in ∼35 s. The TLC - DLTV ICP MS coupling is demonstrated on the separation of four cobalamins (hydroxo-; adenosyl-; cyano-; and methylcobalamin) with limits of detection ∼2 pg and repeatability ∼15% for each individual species.
authors.affiliation: Department of Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
authors.forename: Antonín
authors.initials: A
authors.lastname: Bednařík
authors.affiliation: Department of Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
authors.forename: Iva
authors.initials: I
authors.lastname: Tomalová
authors.affiliation: Department of Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic; Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czech Republic.
authors.forename: Viktor
authors.initials: V
authors.lastname: Kanický
authors.affiliation: Department of Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic; Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czech Republic. Electronic address: preisler@chemi.muni.cz.
authors.forename: Jan
authors.initials: J
authors.lastname: Preisler
mesh_terms: D002855:Chromatography, Thin Layer / Q000379:methods*; D019587:Dietary Supplements; D054023:Lasers, Semiconductor; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D014805:Vitamin B 12 / Q000302:isolation & purification; D014815:Vitamins / Q000302:isolation & purification; D014835:Volatilization
chemical_list: D014815:Vitamins; D014805:Vitamin B 12
keywords: Cobalamin; Diode laser thermal vaporization; Hyphenated techniques; Inductively coupled plasma mass spectrometry; Speciation; Thin-layer chromatography