title: A sentinel protein assay for simultaneously quantifying cellular processes.
abstract: We describe a proteomic screening approach based on the concept of sentinel proteins, biological markers whose change in abundance characterizes the activation state of a given cellular process. Our sentinel assay simultaneously probed 188 biological processes in Saccharomyces cerevisiae exposed to a set of environmental perturbations. The approach can be applied to analyze responses to large sets of uncharacterized perturbations in high throughput.
authors.affiliation: Institute of Biochemistry, Department of Biology, ETH Zurich, Zurich, Switzerland.
authors.forename: Martin
authors.initials: M
authors.lastname: Soste
authors.affiliation: 1] Institute of Biochemistry, Department of Biology, ETH Zurich, Zurich, Switzerland. [2] Laboratory of Applied Proteome Analyses, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libechov, Czech Republic.
authors.forename: Rita
authors.initials: R
authors.lastname: Hrabakova
authors.affiliation: Institute of Molecular Life Sciences and Swiss Institute of Bioinformatics, University of Zurich, Zurich, Switzerland.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Wanka
authors.affiliation: Institute of Biochemistry, Department of Biology, ETH Zurich, Zurich, Switzerland.
authors.forename: Andre
authors.initials: A
authors.lastname: Melnik
authors.affiliation: Institute of Biochemistry, Department of Biology, ETH Zurich, Zurich, Switzerland.
authors.forename: Paul
authors.initials: P
authors.lastname: Boersema
authors.affiliation: Institute of Molecular Systems Biology, Department of Biology, ETH Zurich, Zurich, Switzerland.
authors.forename: Alessio
authors.initials: A
authors.lastname: Maiolica
authors.affiliation: Institute of Biochemistry, Department of Biology, ETH Zurich, Zurich, Switzerland.
authors.forename: Timon
authors.initials: T
authors.lastname: Wernas
authors.affiliation: Institute of Biochemistry, Department of Biology, ETH Zurich, Zurich, Switzerland.
authors.forename: Marco
authors.initials: M
authors.lastname: Tognetti
authors.affiliation: Institute of Molecular Life Sciences and Swiss Institute of Bioinformatics, University of Zurich, Zurich, Switzerland.
authors.forename: Christian
authors.identifier: 0000000177349102
authors.initials: C
authors.lastname: von Mering
authors.affiliation: Institute of Biochemistry, Department of Biology, ETH Zurich, Zurich, Switzerland.
authors.forename: Paola
authors.initials: P
authors.lastname: Picotti
mesh_terms: D019295:Computational Biology / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000737:chemistry; D010750:Phosphoproteins / Q000737:chemistry; D025941:Protein Interaction Mapping; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D049490:Systems Biology / Q000379:methods*; D059467:Transcriptome
chemical_list: D010455:Peptides; D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins