title: To pool or not to pool? Impact of the use of individual and pooled fecal samples for in vitro fermentation studies.
abstract: This study investigated the stability and the activity of the microbiota from a single and a pool of donors in the TNO in vitro model of the colon (TIM-2 system). Our findings demonstrate the suitability of the preparation of a pool of fecal sample to be used for fermentation experiments.
authors.affiliation: Top Institute of Food & Nutrition (TIFN), PO Box 557, 6700 AA Wageningen, The Netherlands; Maastricht University, School of Nutrition, Toxicology and Metabolism (NUTRIM), Faculty of Health, Medicine and Life Sciences, Department of Human Biology, PO Box 616, 6200 MD Maastricht, The Netherlands; The Netherlands Organization for Applied Scientific Research (TNO), PO Box 360, 3700 AJ Zeist, The Netherlands. Electronic address: marisol.aguirremorales@tno.nl.
authors.forename: Marisol
authors.initials: M
authors.lastname: Aguirre
authors.affiliation: Top Institute of Food & Nutrition (TIFN), PO Box 557, 6700 AA Wageningen, The Netherlands; Wageningen University, Laboratory of Microbiology, Systems and Synthetic Biology, 6703 HB Wageningen, The Netherlands. Electronic address: javier.ramirogarcia@wur.nl.
authors.forename: Javier
authors.initials: J
authors.lastname: Ramiro-Garcia
authors.affiliation: The Netherlands Organization for Applied Scientific Research (TNO), PO Box 360, 3700 AJ Zeist, The Netherlands. Electronic address: marjorie_koenen@hotmail.com.
authors.forename: Marjorie E
authors.initials: ME
authors.lastname: Koenen
authors.affiliation: Top Institute of Food & Nutrition (TIFN), PO Box 557, 6700 AA Wageningen, The Netherlands; The Netherlands Organization for Applied Scientific Research (TNO), PO Box 360, 3700 AJ Zeist, The Netherlands. Electronic address: koen.venema@outlook.com.
authors.forename: Koen
authors.initials: K
authors.lastname: Venema
mesh_terms: D000085:Acetates / Q000378:metabolism; D000328:Adult; D044822:Biodiversity; D002087:Butyrates / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D005260:Female; D005285:Fermentation; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D054892:Metagenome; D064307:Microbiota; D008875:Middle Aged; D010802:Phylogeny; D011422:Propionates / Q000378:metabolism; D013997:Time Factors
chemical_list: D000085:Acetates; D002087:Butyrates; D011422:Propionates
keywords: Fecal inoculum preparation; Fermentation; Gut microbiota; In vitro model; Pooling