title: The specific diagnosis of gastrointestinal nematode infections in livestock: larval culture technique, its limitations and alternative DNA-based approaches.
abstract: The specific diagnosis of gastrointestinal nematode infections in ruminants is routinely based on larval culture technique and on the morphological identification of developed third-stage larvae. However, research on the ecology and developmental requirements of different species suggests that environmental conditions (e.g., temperature and humidity) for optimal development to occur vary between the different species. Thus, employing a common culture protocol for all species will favour the development of certain species over others and can cause a biased result in particular when species proportions in a mixed infection are to be determined. Furthermore, the morphological identification of L3 larvae is complicated by a lack of distinctive, obvious features that would allow the identification of all key species. In the present paper we review in detail the potential limitations of larval culture technique and morphological identification and provide account to some modern molecular alternatives to the specific diagnosis of gastrointestinal nematode infection in ruminants.
authors.affiliation: AusDiagnostics Pty. Ltd., Beaconsfield 2015, NSW, Australia. Electronic address: florian@ausdx.com.
authors.forename: Florian
authors.initials: F
authors.lastname: Roeber
authors.affiliation: Environmental and Rural Science, University of New England, Armidale 2351, NSW, Australia.
authors.forename: Lewis
authors.initials: L
authors.lastname: Kahn
mesh_terms: D000818:Animals; D046508:Culture Techniques; D018509:DNA, Helminth / Q000235:genetics; D005767:Gastrointestinal Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D007814:Larva; D058751:Livestock / Q000469:parasitology*; D009348:Nematoda / Q000235:genetics / Q000302:isolation & purification*; D009349:Nematode Infections / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D012418:Ruminants / Q000469:parasitology*
chemical_list: D018509:DNA, Helminth
keywords: Diagnosis; Epidemiology; Larval culture; PCR; Ruminants; Trichostrongylid nematodes