title: One-step synthesis of mussel-inspired molecularly imprinted magnetic polymer as stationary phase for chip-based open tubular capillary electrochromatography enantioseparation.
abstract: A facile approach for preparation of molecularly imprinted polymers was developed and successfully used as chiral stationary phase for rapid enantioseparation by open tubular capillary electrochromatography (OT-CEC). In this work, molecularly imprinted polymers were one-step prepared employing Fe3O4 nanoparticles (NPs) as the supporting substrate and dopamine as the functional monomer. By simply mixing Fe3O4 NPs with template molecules in a weak alkaline solution of dopamine, a thin adherent polydopamine (PDA) film imprinted with template molecules was formed by the self-polymerization of dopamine on the surface of Fe3O4 NPs. After extracting the embedded template molecules, the produced imprinted Fe3O4@PDA NPs are of three dimensional shape of template molecules favoring high binding capacity and magnetism property for easy manipulation. The imprinted Fe3O4@PDA NPs prepared with l-tryptophan, l-tyrosine, Gly-l-Phe or s-ofloxacin as template molecules were packed in the PDMS microchannel via magnetic field as novel stationary phase for the successful enantioseparation of corresponding target analysts. In addition, the imprinted Fe3O4@PDA NPs-based OT-CEC system exhibited excellent reproducibility, stability and repeatability, which provides a powerful protocol for separation enantiomers within a short analytical time and opens up a promising avenue for high-throughput screening of chiral compounds.
authors.affiliation: Department of Chemistry, Nanchang University, Nanchang, China.
authors.forename: Xiao-Ni
authors.initials: XN
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Nanchang University, Nanchang, China. Electronic address: liangrp@163.com.
authors.forename: Ru-Ping
authors.initials: RP
authors.lastname: Liang
authors.affiliation: Department of Chemistry, Nanchang University, Nanchang, China.
authors.forename: Xiang-Ying
authors.initials: XY
authors.lastname: Meng
authors.affiliation: Department of Chemistry, Nanchang University, Nanchang, China. Electronic address: jdqiu@ncu.edu.cn.
authors.forename: Jian-Ding
authors.initials: JD
authors.lastname: Qiu
mesh_terms: D053801:Capillary Electrochromatography / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D060328:Magnetic Phenomena; D054802:Molecular Imprinting / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D013237:Stereoisomerism
chemical_list: D005290:Ferric Compounds; D007211:Indoles; D011108:Polymers; C568283:polydopamine; C000499:ferric oxide
keywords: Enantioseparation; Magnetic nanoparticles; Molecularly imprinted polymer; PDMS microchip; Polydopamine