title: In vivo residue-specific dopa-incorporated engineered mussel bioglue with enhanced adhesion and water resistance.
abstract: Misaminoacylation of 3,4-dihydroxyphenylalanine (Dopa) molecules to tRNA(Tyr) by endogenous tyrosyl-tRNA synthetase allowed the quantitative replacement of tyrosine residues with a yield of over 90 % by an in vivo residue-specific incorporation strategy, to create, for the first time, engineered mussel adhesive proteins (MAPs) in Escherichia coli with a very high Dopa content, close to that of natural MAPs. The Dopa-incorporated MAPs exhibited a superior surface adhesion and water resistance ability by assistance of Dopa-mediated interactions including the oxidative Dopa cross-linking, and furthermore, showed underwater adhesive properties comparable to those of natural MAPs. These results propose promising use of Dopa-incorporated engineered MAPs as bioglues or adhesive hydrogels for practical underwater applications.
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784 (Korea).
authors.forename: Byeongseon
authors.initials: B
authors.lastname: Yang
authors.forename: Niraikulam
authors.initials: N
authors.lastname: Ayyadurai
authors.forename: Hyungdon
authors.initials: H
authors.lastname: Yun
authors.forename: Yoo Seong
authors.initials: YS
authors.lastname: Choi
authors.forename: Byeong Hee
authors.initials: BH
authors.lastname: Hwang
authors.forename: Jun
authors.initials: J
authors.lastname: Huang
authors.forename: Qingye
authors.initials: Q
authors.lastname: Lu
authors.forename: Hongbo
authors.initials: H
authors.lastname: Zeng
authors.forename: Hyung Joon
authors.initials: HJ
authors.lastname: Cha
mesh_terms: D000268:Adhesiveness; D000595:Amino Acid Sequence; D000818:Animals; D032701:Biomimetics; D049872:Bivalvia / Q000737:chemistry* / Q000235:genetics; D004295:Dihydroxyphenylalanine / Q000737:chemistry* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D014867:Water / Q000737:chemistry
chemical_list: D011506:Proteins; D011994:Recombinant Proteins; C037403:adhesive protein, mussel; D014867:Water; D004295:Dihydroxyphenylalanine
keywords: Dopa; biomimetic synthesis; mussel adhesive proteins; noncanonical amino acids; underwater bioadhesive