title: Effects of Cordyceps militaris on the growth of rumen microorganisms and in vitro rumen fermentation with respect to methane emissions.
abstract: This experiment was designed to investigate the effects of different concentrations (0.00, 0.10, 0.15, 0.20, 0.25, and 0.30 g/L) of dried Cordyceps militaris mushroom on in vitro anaerobic ruminal microbe fermentation and methane production using soluble starch as a substrate. Ruminal fluids were collected from Korean native cattle, mixed with phosphate buffer (1:2), and incubated anaerobically at 38 °C for 3, 6, 9, 12, 24, 36, 48, and 72 h. The addition of C. militaris significantly increased total volatile fatty acid and total gas production. The molar proportion of acetate was decreased and that of propionate was increased, with a corresponding decrease in the acetate:propionate ratio. As the concentration of C. militaris increased from 0.10 to 0.30 g/L, methane and hydrogen production decreased. The decrease in methane accumulation relative to the control was 14.1, 22.0, 24.9, 39.7, and 40.9% for the 0.10, 0.15, 0.20, 0.25, and 0.30 g/L treatments, respectively. Ammonia-N concentration and numbers of live protozoa decreased linearly with increasing concentrations of C. militaris. The pH of the medium significantly decreased at the highest level of C. militaris compared with the control. In conclusion, C. militaris stimulated mixed ruminal microorganism fermentation and inhibited methane production in vitro. Therefore, C. militaris could be developed as a novel compound for antimethanogenesis.
authors.affiliation: Department of Beef and Dairy Science, Korea National College of Agriculture and Fisheries, Hwaseong, 445-893, Korea.
authors.forename: W Y
authors.initials: WY
authors.lastname: Kim
authors.affiliation: Virginia Polytechnic Institute and State University, Blacksburg 24061.
authors.forename: M D
authors.initials: MD
authors.lastname: Hanigan
authors.affiliation: Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University, IALS, Jinju, 660-701, Korea.
authors.forename: S J
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: National Institute of Animal Science, Rural Development Association, Suwon, 441-706 Korea.
authors.forename: S M
authors.initials: SM
authors.lastname: Lee
authors.affiliation: National Institute of Animal Science, Rural Development Association, Suwon, 441-706 Korea.
authors.forename: D H
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University, IALS, Jinju, 660-701, Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Hyun
authors.affiliation: Department of Beef and Dairy Science, Korea National College of Agriculture and Fisheries, Hwaseong, 445-893, Korea.
authors.forename: J M
authors.initials: JM
authors.lastname: Yeo
authors.affiliation: Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University, IALS, Jinju, 660-701, Korea,. Electronic address: lss@gnu.ac.kr.
authors.forename: S S
authors.initials: SS
authors.lastname: Lee
mesh_terms: D000818:Animals; D002417:Cattle / Q000378:metabolism* / Q000382:microbiology*; D032661:Cordyceps / Q000502:physiology*; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005285:Fermentation; D008697:Methane / Q000378:metabolism*; D011897:Random Allocation; D012417:Rumen / Q000378:metabolism / Q000382:microbiology*
chemical_list: D005232:Fatty Acids, Volatile; D008697:Methane
keywords: Cordyceps militaris; methane; protozoa; rumen fermentation