title: Substance P and calcitonin gene-related peptide expression in dorsal root ganglia in sciatic nerve injury rats.
abstract: The neuropeptides, substance P and calcitonin gene-related peptide, have been shown to be involved in pain transmission and repair of sciatic nerve injury. A model of sciatic nerve defect was prepared by dissecting the sciatic nerve at the middle, left femur in female Sprague Dawley rats. The two ends of the nerve were encased in a silica gel tube. L5 dorsal root ganglia were harvested 7, 14 and 28 days post sciatic nerve injury for immunohistochemical staining. Results showed that substance P and citonin gene-related peptide expression increased significantly in dorsal root ganglion of rats with sciatic nerve injury. This increase peaked at 7 days, declined at 14 days, and reduced to normal levels by 28 days post injury. The findings indicate that the neuropeptides, substance P and calcitonin gene- related peptide, mainly increased in the early stages after sciatic nerve injury.
authors.affiliation: Department of Orthopedics, First Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China.
authors.forename: Changma
authors.initials: C
authors.lastname: Fu
authors.affiliation: Department of Orthopedics, First Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China.
authors.forename: Zongsheng
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Department of Orthopedics, First Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China.
authors.forename: Defu
authors.initials: D
authors.lastname: Yu
authors.affiliation: Third Clinical College, Anhui Medical University, Hefei 230022, Anhui Province, China.
authors.forename: Zuhua
authors.initials: Z
authors.lastname: Yang
keywords: calcitonin gene-related peptide; dorsal root ganglion; grants-supported paper; neural regeneration; neuropeptides; neuroprotection; neuroregeneration; pain; peripheral nerve injury; sciatic nerve; spinal cord; substance P