title: Preventive effects ofDendrobium candidumWall ex Lindl. on the formation of lung metastases in BALB/c mice injected with 26-M3.1 colon carcinoma cells.
abstract: Dendrobium candidumWall ex Lindl. (D. candidum) is a traditional Chinese medicine widely used in Asia. The present study has showed thatD. candidumexerted an anti-metastatic effect in mice injected with 26-M3.1 colon carcinoma cells.D. candidumshowed the most marked tumor inhibitory rate of 64.5% at a dose of 400 mg/kg body weight (b.w). The mRNA and protein expression of Bax in lung tissue ofD. candidum-treated mice was shown to be higher as compared with control mice, whereas the mRNA and protein expression of Bcl-2 showed the opposite trend. Decreased mRNA and protein expression of MMP and increased expression of TIMPs was demonstrated in lung tissues by quantitative polymerase chain reaction and western blot assays.D. candidumreduced the serum cytokine levels of IL-6, IL-12, TNF-α and IFN-γ to a greater extent as compared with the control mice, and administration of 400 mg/kg b.w. resulted in a lower serum cytokine levels as compared with mice treated with 200 mg/kg b.w. Eleven compounds were in theD. candidumleaf, of which the functional contents may help to generate novel treatments for the prevention of lung metastases. The results of the present study have demonstrated thatD. candidumhad a potentin vivoantitumor and anti-metastatic effect in BALB/c mice injected with 26-M3.1 cells.
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China ; Department of Food, Nutrition and Packaging Science, Clemson University, Clemson, SC 29634, USA.
authors.forename: Guijie
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Sun
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China.
authors.forename: Yalin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Food, Nutrition and Packaging Science, Clemson University, Clemson, SC 29634, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
keywords: Dendrobium candidum; anti-metastasis; anticancer; apoptosis; cytokine