title: Antenatal taurine reduces cerebral cell apoptosis in fetal rats with intrauterine growth restriction.
abstract: From pregnancy to parturition, Sprague-Dawley rats were daily administered a low protein diet to establish a model of intrauterine growth restriction. From the 12(th) day of pregnancy, 300 mg/kg rine was daily added to food until spontaneous delivery occurred. Brain tissues from normal neonatal rats at 6 hours after delivery, neonatal rats with intrauterine growth restriction, and neonatal rats with intrauterine growth restriction undergoing taurine supplement were obtained for further experiments. The terminal deoxyribonucleotidyl transferase (TdT)-mediated biotin-16-dUTP nick-end labeling assay revealed that the number of apoptotic cells in the brain tissue of neonatal rats with intrauterine growth restriction significantly increased. Taurine supplement in pregnant rats reduced cell apoptosis in brain tissue from neonatal rats with intrauterine growth restriction. nohistochemical staining revealed that taurine supplement increased glial cell line-derived neurotrophic factor expression and decreased caspase-3 expression in the cerebral cortex of intrauterine growth-restricted fetal rats. These results indicate that taurine supplement reduces cell apoptosis through the glial cell line-derived neurotrophic factor-caspase-3 signaling pathway, resulting in a protective effect on the intrauterine growth-restricted fetal rat brain.
authors.affiliation: Department of Neonatology and NICU of Bayi Children's Hospital, General Hospital of Beijing Military Command, Beijing 100700, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Neonatology and NICU of Bayi Children's Hospital, General Hospital of Beijing Military Command, Beijing 100700, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Neonatology and NICU of Bayi Children's Hospital, General Hospital of Beijing Military Command, Beijing 100700, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Neonatology and NICU of Bayi Children's Hospital, General Hospital of Beijing Military Command, Beijing 100700, China.
authors.forename: Na
authors.initials: N
authors.lastname: Yang
authors.affiliation: Department of Neonatology and NICU of Bayi Children's Hospital, General Hospital of Beijing Military Command, Beijing 100700, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Neonatology and NICU of Bayi Children's Hospital, General Hospital of Beijing Military Command, Beijing 100700, China.
authors.forename: Xiaotun
authors.initials: X
authors.lastname: Ren
keywords: brain; caspase-3; cell tosis; fetal rats; glial cell line-derived neurotrophic factor; grants-supported paper; intrauterine growth restriction; neural cells; neural development; neural regeneration; neuroregeneration; taurine