title: Cost-effective microsatellite markers for Banksia integrifolia (Proteaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed to assess the level of genetic variation and population structure in Banksia integrifolia, a widespread species endemic to eastern Australia. •
  
  
  RESULTS
  We used next-generation sequencing approaches to identify and develop 11 polymorphic microsatellite markers with perfect tri- and tetranucleotide repeats. We tested these markers with 71 specimens from three populations. Observed and expected heterozygosities ranged from 0.0 to 0.875 and 0.0 to 0.763, respectively. •
  
  
  CONCLUSIONS
  The developed markers will be valuable for studies of the population structure, mating system, and selection of provenances for restoration projects involving B. integrifolia.
authors.affiliation: Ecosystem Management, University of New England, Armidale, New South Wales 2351, Australia.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Fatemi
authors.affiliation: Ecological Genetics, Landcare Research, Gerald Street, Lincoln 7640, New Zealand.
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Houliston
authors.affiliation: Ecosystem Management, University of New England, Armidale, New South Wales 2351, Australia.
authors.forename: Azadeh
authors.initials: A
authors.lastname: Haddadchi
authors.affiliation: Ecosystem Management, University of New England, Armidale, New South Wales 2351, Australia.
authors.forename: Caroline L
authors.initials: CL
authors.lastname: Gross
keywords: Banksia integrifolia; Proteaceae; coast banksia; genetic variation; next-generation sequencing; simple sequence repeat markers