title: Rapid determination of memantine in human plasma by using nanoring carboxyl-functionalized paramagnetic molecularly imprinted polymer d-μ-SPE and UFLC-MS/MS.
abstract: A novel, simple, and sensitive method based on the use of dispersive micro-solid-phase extraction (d-μ-SPE) procedure combined with ultra-fast liquid chromatography-tandem quadrupole mass spectrometry (UFLC-MS/MS) for the determination of memantine (ME) was developed and validated over the linearity range 0.05-10.0 µg/L with 100 μL of human plasma using memantine-D6 (ME-D6) as the internal standard. The novel nanoring carboxyl-functionalized paramagnetic molecularly imprinted polymer (NR-CF-Mag-MIP) was synthesized by ultrasound-assisted suspension polymerization, using ME as a template molecule, methacrylic acid as a functional monomer, and divinylbenzene as a cross-linking agent. The NR-CF-Mag-MIP was used as the d-μ-SPE sorbent to extract ME from human plasma samples. The obtained results demonstrated the higher extraction capacity of NR-CF-Mag-MIP with recoveries between 97.6 and 101%. The limits of quantification (LOQs) for ME was 0.015 µg/L. Validation results on linearity, specificity, accuracy, precision, and stability, as well as on application to the analysis of samples taken up to 480 h after oral administration of 20 mg (two 10 mg capsules) of ME in healthy volunteers demonstrated the applicability to bioequivalence studies.
authors.affiliation: Ningbo First Hospital, Ningbo, Zhejiang, 315010, China.
authors.forename: Hai-Wen
authors.initials: HW
authors.lastname: Qiu
authors.affiliation: Chaohu Hospital of Anhui Medical University, Chaohu, Anhui, 238000, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Xia
authors.affiliation: Ningbo First Hospital, Ningbo, Zhejiang, 315010, China.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Gong
authors.affiliation: Ningbo First Hospital, Ningbo, Zhejiang, 315010, China.
authors.forename: Lie-Min
authors.initials: LM
authors.lastname: Ruan
authors.affiliation: Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang, 315010, China.
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Zhao
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000031:analogs & derivatives / Q000737:chemistry; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008297:Male; D008559:Memantine / Q000032:analysis* / Q000097:blood; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D058185:Magnetite Nanoparticles; D008689:Methacrylates; C518066:poly(hydroxyethyl methacrylate-N-methacryloylcysteine); D003545:Cysteine; D008559:Memantine
keywords: dispersive micro-solid-phase extraction (d-μ-SPE); human plasma; memantine (ME); nanoring carboxyl-functionalized paramagnetic molecularly imprinted polymer (NR-CF-Mag-MIP); ultra-fast liquid chromatography-tandem quadrupole mass spectrometry (UFLC-MS/MS)