title: Lab-on-chip cytometry based on magnetoresistive sensors for bacteria detection in milk.
abstract: Flow cytometers have been optimized for use in portable platforms, where cell separation, identification and counting can be achieved in a compact and modular format. This feature can be combined with magnetic detection, where magnetoresistive sensors can be integrated within microfluidic channels to detect magnetically labelled cells. This work describes a platform for in-flow detection of magnetically labelled cells with a magneto-resistive based cell cytometer. In particular, we present an example for the validation of the platform as a magnetic counter that identifies and quantifies Streptococcus agalactiae in milk.
authors.affiliation: INESC-MN-Instituto de Engenharia de Sistemas e Computadores-Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, Portugal. acfernandes@inesc-mn.pt.
authors.forename: Ana C
authors.initials: AC
authors.lastname: Fernandes
authors.affiliation: INESC-MN-Instituto de Engenharia de Sistemas e Computadores-Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, Portugal. admargduarte@fmv.ulisboa.pt.
authors.forename: Carla M
authors.initials: CM
authors.lastname: Duarte
authors.affiliation: INESC-MN-Instituto de Engenharia de Sistemas e Computadores-Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, Portugal. fcardoso@inesc-mn.pt.
authors.forename: Filipe A
authors.initials: FA
authors.lastname: Cardoso
authors.affiliation: CIISA, Veterinary Medicine Faculty, Technical University of Lisbon, Av. da Universidade Técnica, 1300-477 Lisbon, Portugal. ricardobexiga@fmv.ulisboa.pt.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Bexiga
authors.affiliation: INESC-MN-Instituto de Engenharia de Sistemas e Computadores-Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, Portugal. scardoso@inesc-mn.pt.
authors.forename: Susana
authors.initials: S
authors.lastname: Cardoso
authors.affiliation: INESC-MN-Instituto de Engenharia de Sistemas e Computadores-Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, Portugal. pfreitas@inesc-mn.pt.
authors.forename: Paulo P
authors.initials: PP
authors.lastname: Freitas
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification* / Q000472:pathogenicity; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008892:Milk / Q000382:microbiology