title: Determination of phenolic acids and flavonoids in raw propolis by silica-supported ionic liquid-based matrix solid phase dispersion extraction high performance liquid chromatography-diode array detection.
abstract: The silica-supported ionic liquid (S-SIL) was prepared by impregnation and used as the dispersion adsorbent of matrix solid phase dispersion (MSPD) for the simultaneous extraction of eight phenolic acids and flavonoids, including caffeic acid, ferulic acid, morin, luteolin, quercetin, apigenin, chrysin, and kaempferide in raw propolis. High performance liquid chromatography with a Zorbax SB-C18 column (150mm×4.6mm, 3.5μm) was used for separation of the analytes. The mobile phase consisted of 0.2% phosphoric acid aqueous solution and acetonitrile and the flow rate of the mobile phase was 0.5mL/min. The experimental conditions for silica-supported ionic liquid-based matrix solid phase dispersion (S-SIL-based MSPD) were optimized. S-SIL containing 10% [C6MIM]Cl was used as dispersant, 20mL of n-hexane as washing solvent and 15mL of methanol as elution solvent. The ratio of S-SIL to sample was selected to be 4:1. The standard curves showed good linear relationship (r>0.9995). The limits of detection and quantification were in the range of 5.8-22.2ngmL(-1) and 19.2-74.0ngmL(-1), respectively. The relative standard deviations (RSDs) of intra-day and inter-day determination were lower than 8.80% and 11.19%, respectively. The recoveries were between 65.51% and 92.32% with RSDs lower than 8.95%. Compared with ultrasound-assisted extraction (UAE) and soxhlet extraction, the present method consumed less sample, organic solvent, and extraction time, although the extraction yields obtained by S-SIL-based MSPD are slightly lower than those obtained by UAE.
authors.affiliation: College of Chemistry and Life Science, Changchun University of Technology, Changchun 130012, China; College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Zhibing
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Chemistry and Life Science, Changchun University of Technology, Changchun 130012, China; School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Sun
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Yuanpeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Lei
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Aimin
authors.initials: A
authors.lastname: Yu
authors.affiliation: College of Chemistry and Life Science, Changchun University of Technology, Changchun 130012, China. Electronic address: analchem@jlu.edu.cn.
authors.forename: Fangping
authors.initials: F
authors.lastname: Qiu
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Hanqi
authors.initials: H
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D052578:Ionic Liquids / Q000737:chemistry; D010636:Phenols / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D011429:Propolis / Q000737:chemistry*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D005419:Flavonoids; D052578:Ionic Liquids; D010636:Phenols; D011429:Propolis
keywords: High-performance liquid chromatography (HPLC); Phenolic acids and flavonoids; Raw propolis; Silica-supported ionic liquid-based matrix solid phase dispersion (S-SIL-based MSPD)