title: Is acetylcholinesterase a biomarker of susceptibility in Daphnia magna (Crustacea, Cladocera) after deltamethrin exposure?
abstract: In the present study, we explored the possibility of using the acetylcholinesterase (AChE) as a biomarker after deltamethrin (pyrethroid insecticide) exposure with three strains of the cladoceran Daphnia magna. Four calculated time-weighted deltamethrin concentrations (20.1, 40.3, 80.6 and 161.3 ng L(-1)) were compared against control acetylcholinesterase activity. Our results showed that after 48 h of deltamethrin exposure, all treatments induced a significant decrease of AChE activities whatever the three considered strains. However, diverse responses were registered in terms of lowest observed effect concentrations (LOEC: 80.6 ng L(-1) for strain 1 and 20.1 ng L(-1) for strains 2 and 3) revealing differences in sensitivity among the three tested strains of D. magna. Our results suggest that after deltamethrin exposure, the AChE activity responses can be also used as a biomarker of susceptibility (i.e., variation of strain specific response). Moreover, our results show that strain 1 is the less sensitive in terms of IC50-48 h of AChE, whereas it became the most sensitive when considering the EC50-48 h estimated in the standard ecotoxicity test.
authors.affiliation: Laboratoire de Bio-surveillance de l'Environnement (LBE), Unité d'Hydrobiologie littorale et limnique, Université de Carthage, Faculté des Sciences de Bizerte, 7021 Zarzouna, Bizerte, Tunisia; Université de Lorraine (UdL), Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC) - UMR 7360 CNRS, Campus Bridoux, Bât. IBiSE, 8, rue du Général Delestraint, 57070 Metz, France.
authors.forename: Héla
authors.initials: H
authors.lastname: Toumi
authors.affiliation: Laboratoire de Bio-surveillance de l'Environnement (LBE), Unité d'Hydrobiologie littorale et limnique, Université de Carthage, Faculté des Sciences de Bizerte, 7021 Zarzouna, Bizerte, Tunisia.
authors.forename: Moncef
authors.initials: M
authors.lastname: Boumaiza
authors.affiliation: ICPEES (UMR 7515 CNRS - Université de Strasbourg), 1 rue Blessig, 67084 Strasbourg Cedex, France.
authors.forename: Maurice
authors.initials: M
authors.lastname: Millet
authors.affiliation: Laboratório de Remediação Ambiental, Universidade do Vale do Itajaí, Rua Uruguai, 458, Itajaí, SC 88302-202, Brazil.
authors.forename: Claudemir Marcos
authors.initials: CM
authors.lastname: Radetski
authors.affiliation: Université de Lorraine (UdL), Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC) - UMR 7360 CNRS, Campus Bridoux, Bât. IBiSE, 8, rue du Général Delestraint, 57070 Metz, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Felten
authors.affiliation: Université de Lorraine (UdL), Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC) - UMR 7360 CNRS, Campus Bridoux, Bât. IBiSE, 8, rue du Général Delestraint, 57070 Metz, France. Electronic address: jean-francois.ferard@univ-lorraine.fr.
authors.forename: Jean François
authors.initials: JF
authors.lastname: Férard
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003621:Daphnia / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D007306:Insecticides / Q000633:toxicity*; D009570:Nitriles / Q000633:toxicity*; D011722:Pyrethrins / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D000110:Acetylcholinesterase
keywords: Acetylcholinesterase; Biomarker; Daphnia magna; Inter-strain sensitivity; Pyrethroid