title: Fe/S protein biogenesis in trypanosomes - A review.
abstract: Trypanosoma brucei, the causative agent of the African sleeping sickness of humans, and other kinetoplastid flagellates belong to the eukarytotic supergroup Excavata. This early-branching model protist is known for a broad range of unique features. As it is amenable to most techniques of forward and reverse genetics, T. brucei was subject to several studies of its iron-sulfur (Fe/S) protein biogenesis and thus represents the best studied excavate eukaryote. Here we review what is known about the Fe/S protein biogenesis of T. brucei, and focus especially on the comparative and evolutionary interesting aspects. We also explore the connections between the well-known and quite conserved ISC and CIA machineries and the tRNA thiolation pathway. Moreover, the Fe/S cluster protein biogenesis is dissected in the procyclic stage of T. brucei which has an active mitochondrion, as well as in its pathogenic bloodstream stage with a metabolically repressed organelle. This article is part of a Special Issue entitled: Fe/S proteins: Analysis, structure, function, biogenesis and diseases.
authors.affiliation: Biology Centre, Institute of Parasitology, Czech Academy of Sciences and Faculty of Science, University of South Bohemia, 37005 České Budějovice (Budweis), Czech Republic. Electronic address: jula@paru.cas.cz.
authors.forename: Julius
authors.initials: J
authors.lastname: Lukeš
authors.affiliation: Biology Centre, Institute of Parasitology, Czech Academy of Sciences and Faculty of Science, University of South Bohemia, 37005 České Budějovice (Budweis), Czech Republic.
authors.forename: Somsuvro
authors.initials: S
authors.lastname: Basu
mesh_terms: D053898:Biosynthetic Pathways; D006801:Humans; D007501:Iron / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000096:biosynthesis*; D008928:Mitochondria / Q000378:metabolism; D015800:Protozoan Proteins / Q000096:biosynthesis*; D012343:RNA, Transfer / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism; D014346:Trypanosoma brucei brucei / Q000378:metabolism*; D014353:Trypanosomiasis, African / Q000469:parasitology
chemical_list: D007506:Iron-Sulfur Proteins; D015800:Protozoan Proteins; D013455:Sulfur; D012343:RNA, Transfer; D007501:Iron
keywords: Fe/S cluster; Kinetoplastida; Protist; Trypanosoma brucei