title: Albumin antioxidant response to stress in diabetic nephropathy progression.
abstract: BACKGROUND
  A new component of the protein antioxidant capacity, designated Response Surplus (RS), was recently described. A major feature of this component is the close relationship between protein antioxidant capacity and molecular structure. Oxidative stress is associated with renal dysfunction in patients with renal failure, and plasma albumin is the target of massive oxidation in nephrotic syndrome and diabetic nephropathy. The aim of the present study was to explore the albumin redox state and the RS component of human albumin isolated from diabetic patients with progressive renal damage.
  
  
  RESULTS
  Serum aliquots were collected and albumin isolated from 125 diabetic patients divided into 5 groups according to their estimated glomerular filtration rate (GFR). In addition to clinical and biochemical variables, the albumin redox state, including antioxidant capacity, thiol group content, and RS component, were evaluated. The albumin antioxidant capacity and thiol group content were reciprocally related to the RS component in association with GFR reduction. The GFR decline and RS component were significantly negatively correlated (R = -0.83, p<0.0001). Age, creatinine, thiol groups, and antioxidant capacity were also significantly related to the GFR decline (R = -0.47, p < 0.001; R = -0.68, p<0.0001; R = 0.44, p < 0.001; and R = 0.72, p < 0.0001).
  
  
  CONCLUSIONS
  The response of human albumin to stress in relation to the progression of diabetic renal disease was evaluated. The findings confirm that the albumin molecular structure is closely related to its redox state, and is a key factor in the progression of diabetes nephropathy.
authors.affiliation: Department of Experimental Metabolism, Center for Biomedical Research of Michoacán (CIBIMI-IMSS), Morelia, Michoacán, México.
authors.forename: Rafael
authors.initials: R
authors.lastname: Medina-Navarro
authors.affiliation: Department of Nephrology, General Regional Hospital N° 1, IMSS, Morelia, Michoacán, Mexico.
authors.forename: Itzia
authors.initials: I
authors.lastname: Corona-Candelas
authors.affiliation: Department of Nephrology, General Regional Hospital N° 1, IMSS, Morelia, Michoacán, Mexico.
authors.forename: Saúl
authors.initials: S
authors.lastname: Barajas-González
authors.affiliation: Biochemistry Medical Research Unit, National Medical Center, IMSS, México City, México.
authors.forename: Margarita
authors.initials: M
authors.lastname: Díaz-Flores
authors.affiliation: Biochemistry Medical Research Unit, National Medical Center, IMSS, México City, México.
authors.forename: Genoveva
authors.initials: G
authors.lastname: Durán-Reyes
mesh_terms: D000328:Adult; D000368:Aged; D000418:Albumins / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism*; D003928:Diabetic Nephropathies / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D005919:Glomerular Filtration Rate / Q000502:physiology; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D018384:Oxidative Stress / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D000418:Albumins; D000975:Antioxidants