title: Conjugated polymer nanoparticles for cell membrane imaging.
abstract: The outstanding optical properties and biocompatibility of fluorescent conjugated polymer nanoparticles (CPNs) make them favorable for bioimaging application. However, few CPNs could achieve stable cell membrane labeling due to cell endocytosis. In this work, conjugated polymer nanoparticles (PFPNP-PLE) encapsulated with PFP and PLGA-PEG-N3 in the matrix and functionalized with the small-molecule drug plerixafor (PLE) on the surface were prepared by a mini-emulsion method. PFPNP-PLE exhibits excellent photophysical properties, low cytotoxicity, and specific cytomembrane location, which makes it a potential cell membrane labeling reagent with blue fluorescence emission, an important component for multilabel/multicolor bioimaging.
authors.affiliation: Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190 (P. R. China).
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Chenyao
authors.initials: C
authors.lastname: Nie
authors.forename: Liheng
authors.initials: L
authors.lastname: Feng
authors.forename: Huanxiang
authors.initials: H
authors.lastname: Yuan
authors.forename: Libing
authors.initials: L
authors.lastname: Liu
authors.forename: Fengting
authors.initials: F
authors.lastname: Lv
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
mesh_terms: D001596:Benzylamines; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D000080027:Cyclams; D004337:Drug Carriers / Q000737:chemistry; D004705:Endocytosis; D005456:Fluorescent Dyes / Q000737:chemistry; D019073:HT29 Cells; D006367:HeLa Cells; D006571:Heterocyclic Compounds / Q000737:chemistry; D006801:Humans; D061986:MCF-7 Cells; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011098:Polyglactin 910 / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001596:Benzylamines; D000080027:Cyclams; D004337:Drug Carriers; D005456:Fluorescent Dyes; D006571:Heterocyclic Compounds; D011108:Polymers; C102418:poly(lactic-glycolic acid)-poly(ethyleneglycol) copolymer; D011098:Polyglactin 910; D011092:Polyethylene Glycols; C088327:plerixafor
keywords: cell membrane imaging; conjugated polymers; fluorescence; nanoparticles; plerixafor