title: Regulation of extracellular signal-regulated kinase 1/2 influences hippocampal neuronal survival in a rat model of diabetic cerebral ischemia.
abstract: Activation of extracellular signal-regulated kinase 1/2 has been demonstrated in acute brain ischemia. We hypothesized that activated extracellular signal-regulated kinase 1/2 can protect hippocampal neurons from injury in a diabetic model after cerebral ischemia/reperfusion. In this study, transient whole-brain ischemia was induced by four-vessel occlusion in normal and diabetic rats, and extracellular signal-regulated kinase 1/2 inhibitor (U0126) was administered into diabetic rats 30 minutes before ischemia as a pretreatment. Results showed that the number of surviving neurons in the hippocampal CA1 region was reduced, extracellular signal-regulated kinase 1/2 phosphorylation and Ku70 activity were decreased, and pro-apoptotic Bax expression was upregulated after intervention using U0126. These findings demonstrate that inhibition of extracellular signal-regulated kinase 1/2 activity aggravated neuronal loss in the hippocampus in a diabetic rat after cerebral ischemia/reperfusion, further decreased DNA repairing ability and accelerated apoptosis in hippocampal neurons. Extracellular signal-regulated kinase 1/2 activation plays a neuroprotective role in hippocampal neurons in a diabetic rat after cerebral ischemia/reperfusion.
authors.affiliation: College of Rehabilitation, Hebei United University, Tangshan, Hebei Province, China.
authors.forename: Yaning
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Affiliated Hospital of Hebei United University, Tangshan, Hebei Province, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Rehabilitation, Hebei United University, Tangshan, Hebei Province, China.
authors.forename: Qiqun
authors.initials: Q
authors.lastname: Tang
authors.affiliation: College of Rehabilitation, Hebei United University, Tangshan, Hebei Province, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Zhang
authors.affiliation: College of Rehabilitation, Hebei United University, Tangshan, Hebei Province, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Jing
authors.affiliation: College of Rehabilitation, Hebei United University, Tangshan, Hebei Province, China.
authors.forename: Changxiang
authors.initials: C
authors.lastname: Chen
authors.affiliation: College of Rehabilitation, Hebei United University, Tangshan, Hebei Province, China.
authors.forename: Shuxing
authors.initials: S
authors.lastname: Li
keywords: Bax; DNA dependent protein kinase; apoptosis; brain injury; cerebral ischemia/reperfusion; extracellular signal-regulated kinase; hippocampus; nerve regeneration; neural regeneration