title: Evaluation of the antioxidant activity and antiproliferative effect of the jaboticaba (Myrciaria cauliflora) seed extracts in oral carcinoma cells.
abstract: It is becoming increasingly evident that certain phytochemicals possess cancer chemopreventive properties. In this study, the antiproliferative activity of extracts from different parts of the jaboticaba (Myrciaria cauliflora) plant was evaluated for its effect on human oral carcinoma cell lines. The cytotoxicities of various plant extract concentrations were examined and the 50% maximal inhibitory concentration (IC50) was determined. Water extracts of jaboticaba seeds showed concentration-dependent antiproliferative effects. Annexin V/propidium iodide positivity with active caspase-3 induction indicated that the treated cells underwent apoptosis. Several important regulatory proteins (Bcl-2, Bcl-xL, Bid, and survivin) involved in apoptosis were also evaluated. The antioxidant activity of jaboticaba was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, and the drug concentration eliciting 50% maximum stimulation (SC50) was determined. The present findings suggest that water extracts of jaboticaba seeds exhibit an antiproliferative effect against oral cancer cells by inducing apoptosis through downregulating survivin expression and thereby activating caspase-mediated Bid cleavage.
authors.affiliation: Department of Otolaryngology, Cathay General Hospital, Taipei City 106, Taiwan ; Department of Otolaryngology, Sijhih Cathay General Hospital, New Taipei City 221, Taiwan ; School of Medicine, Fu-Jen Catholic University, New Taipei City 242, Taiwan.
authors.forename: Wen-Hung
authors.initials: WH
authors.lastname: Wang
authors.affiliation: Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan ; National Sun Yat-Sen University-Kaohsiung Medical University Joint Research Center, Kaohsiung 804, Taiwan ; Translational Research Center, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan ; Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung 804, Taiwan.
authors.forename: Yu-Chang
authors.identifier: 0000-0002-1917-5893
authors.initials: YC
authors.lastname: Tyan
authors.affiliation: Institute of Cosmetic Science, Chia-Nan University of Pharmacy and Science, Tainan 717, Taiwan.
authors.forename: Zong-Shiow
authors.initials: ZS
authors.lastname: Chen
authors.affiliation: Institute of Cosmetic Science, Chia-Nan University of Pharmacy and Science, Tainan 717, Taiwan.
authors.forename: Ching-Gong
authors.initials: CG
authors.lastname: Lin
authors.affiliation: Translational Research Center, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan ; Instrument Technology Research Center, National Applied Research Laboratories, Hsinchu 300, Taiwan ; Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
authors.forename: Ming-Hui
authors.identifier: 0000-0001-5856-0498
authors.initials: MH
authors.lastname: Yang
authors.affiliation: Translational Research Center, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan ; Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan ; Department of Obstetrics and Gynecology, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan ; School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Shyng-Shiou
authors.initials: SS
authors.lastname: Yuan
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan ; Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
authors.forename: Wan-Chi
authors.identifier: 0000-0002-4490-3581
authors.initials: WC
authors.lastname: Tsai
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D051023:BH3 Interacting Domain Death Agonist Protein / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism; D009062:Mouth Neoplasms / Q000378:metabolism / Q000473:pathology*; D027822:Myrtaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D000077022:Survivin; D014867:Water / Q000737:chemistry
chemical_list: D000975:Antioxidants; D051023:BH3 Interacting Domain Death Agonist Protein; C107489:BIRC5 protein, human; D016166:Free Radical Scavengers; D051034:Inhibitor of Apoptosis Proteins; D010936:Plant Extracts; D000077022:Survivin; D014867:Water