title: Visceral Leishmaniasis: Advancements in Vaccine Development via Classical and Molecular Approaches.
abstract: Visceral leishmaniasis (VL) or kala-azar, a vector-borne protozoan disease, shows endemicity in larger areas of the tropical, subtropical and the Mediterranean countries. WHO report suggested that an annual incidence of VL is nearly 200,000 to 400,000 cases, resulting in 20,000 to 30,000 deaths per year. Treatment with available anti-leishmanial drugs are not cost effective, with varied efficacies and higher relapse rate, which poses a major challenge to current kala-azar control program in Indian subcontinent. Therefore, a vaccine against VL is imperative and knowing the fact that recovered individuals developed lifelong immunity against re-infection, it is feasible. Vaccine development program, though time taking, has recently gained momentum with the emergence of omic era, i.e., from genomics to immunomics. Classical as well as molecular methodologies have been overtaken with alternative strategies wherein proteomics based knowledge combined with computational techniques (immunoinformatics) speed up the identification and detailed characterization of new antigens for potential vaccine candidates. This may eventually help in the designing of polyvalent synthetic and recombinant chimeric vaccines as an effective intervention measures to control the disease in endemic areas. This review focuses on such newer approaches being utilized for vaccine development against VL.
authors.affiliation: Division of Parasitology, Central Drug Research Institute , Lucknow , India.
authors.forename: Sumit
authors.initials: S
authors.lastname: Joshi
authors.affiliation: Division of Parasitology, Central Drug Research Institute , Lucknow , India.
authors.forename: Keerti
authors.initials: K
authors.lastname: Rawat
authors.affiliation: Division of Parasitology, Central Drug Research Institute , Lucknow , India.
authors.forename: Narendra Kumar
authors.initials: NK
authors.lastname: Yadav
authors.affiliation: Division of Molecular and Structural Biology, Central Drug Research Institute , Lucknow , India.
authors.forename: Vikash
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Division of Molecular and Structural Biology, Central Drug Research Institute , Lucknow , India.
authors.forename: Mohammad Imran
authors.initials: MI
authors.lastname: Siddiqi
authors.affiliation: Division of Parasitology, Central Drug Research Institute , Lucknow , India.
authors.forename: Anuradha
authors.initials: A
authors.lastname: Dube
keywords: DNA vaccines; mutant vaccines; recombinant vaccines; synthetic peptide vaccines; visceral leishmaniasis