title: Antioxidant potential of Rumex vesicarius L.: in vitro approach.
abstract: OBJECTIVE
  To assess in-vitro antioxidant activity of different fraction and perform high performance thin layer chromatography fingerprint analysis of most active fraction of Rumex vesicarius L. (R. vesicarius).
  
  
  METHODS
  In the present study, acetone, ethyl acetate, n-butanol, and methanol extracts of R. vesicarius were evaluated for radical scavenging activity by studying the inhibition of the level of lipid peroxidation induced by Fe(++)/ascorbate, DNA sugar damage, scavenging of hydrogen peroxide, diphenylphosphine DPPH radical scavenging activity, total phenolic content, total flavonoids content and total proanthocyanidin. High performance thin layer chromatography finger print profiling of R. vesicarius L. was also done.
  
  
  RESULTS
  Lipid peroxidation induced by the iron/ascorbate system, hydrogen peroxide, diphenylphosphine and DNA sugar damage were inhibited by the addition of different extract of R. vesicarius. Among them, methanolic extract showed maximum efficacy. The methanolic extract showed the highest total phenolic, total flavonoids and total proanthocyanidin contents.
  
  
  CONCLUSIONS
  The results suggest that the extracts can be a vital source of phytochemical antioxidants.
authors.affiliation: Department of Pharmacology, College of Pharmacy, Salman Bin Abdulaziz University, Kingdom of Saudi Arabia.
authors.forename: Tajdar Husain
authors.initials: TH
authors.lastname: Khan
authors.affiliation: Department of Pharmacology, College of Pharmacy, Salman Bin Abdulaziz University, Kingdom of Saudi Arabia.
authors.forename: Majid Ahmad
authors.initials: MA
authors.lastname: Ganaie
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, Kingdom of Saudi Arabia.
authors.forename: Nasir Ali
authors.initials: NA
authors.lastname: Siddiqui
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University, Kingdom of Saudi Arabia.
authors.forename: Aftab
authors.initials: A
authors.lastname: Alam
authors.affiliation: Department of Pharmacology, College of Pharmacy, Salman Bin Abdulaziz University, Kingdom of Saudi Arabia.
authors.forename: Mohd Nazam
authors.initials: MN
authors.lastname: Ansari
keywords: DNA sugar damage; DPPH; HPTLC; Rumex vesicarius L.; antioxidant