title: What is the new target inhibiting the progression of Alzheimer's disease.
abstract: To stop the progression of Alzheimer's disease in the early stage, it is necessary to identify new therapeutic targets. We examined striatal-enriched phosphatase 61 expression in the brain tissues of 12-month-old APPswe/PSEN1dE9 transgenic mice. Immunohistochemistry showed that al-enriched phosphatase 61 protein expression was significantly increased but phosphorylated N-methyl-D-aspartate receptor 2B levels were significantly decreased in the cortex and hippocampus of APPswe/PSEN1dE9 transgenic mice. Western blotting of a cell model of Alzheimer's disease consisting of amyloid-beta peptide (1-42)-treated C57BL/6 mouse cortical neurons in vitro showed that valeric acid (AP5), an N-methyl-D-aspartate receptor antagonist, significantly inhibited amyloid-beta 1-42-induced increased activity of striatal-enriched phosphatase 61. In addition, the phosphorylation of N-methyl-D-aspartate receptor 2B at Tyr1472 was impaired in amyloid-beta 1-42-treated cortical neurons, but knockdown of striatal-enriched phosphatase 61 enhanced the phosphorylation of N-methyl-D-aspartate receptor 2B. Collectively, these findings indicate that striatal-enriched phosphatase 61 can disturb N-methyl-D-aspartate receptor transport and inhibit the progression of learning and study disturbances induced by Alzheimer's disease. Thus, al-enriched phosphatase 61 may represent a new target for inhibiting the progression of Alzheimer's disease.
authors.affiliation: Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Provincial Key Laboratory of Cardiovascular and Cerebrovascular Drug Basic Research, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Cao
keywords: Alzheimer's disease; GluN2B; N-methyl-D-aspartate receptor; RNA interference; amyloid-beta peptide; brain injury; immunohistochemistry; neural regeneration; neurodegeneration; neuroregeneration; striatal-enriched phosphatase 61; western blot