title: Passive protection against Salmonella enterica serovar Enteritidis infection from maternally derived antibodies of hens vaccinated with a ghost vaccine.
abstract: This study evaluated maternal immunity against Salmonella enterica serovar Enteritidis acquired through the egg yolk. Two-hundred 19-week-old specific pathogen free (SPF) broiler breeders which were randomly divided into two groups of equal size were injected with S. Enteritidis ghosts (5 × 10(9) colony forming units in 0.1 ml per hen) and phosphate-buffered saline (PBS, 0.01 mol ⋅ l(-1), pH 7.4) twice, respectively, with an interval of 2 weeks. An indirect enzyme-linked immunosorbent assay (ELISA) was applied to detect specific antibodies against S. Enteritidis. S. Enteritidis-specific antibody levels in the vaccinated group increased over time and were significantly higher than those of the control group on days 28 (P < 0.001) and 35 (P < 0.001) post-vaccination. Ten 7-day-old chicks from hens that were vaccinated with a S. Enteritidis ghost vaccine were challenged at 14 days of age with 5 × 10(9) CFU of S. Enteritidis DH091 (homologous to the vaccine strain), 8/10 (80%) chicks from vaccinated hens survived, whereas 3/10 (30%) chicks from unvaccinated hens survived. The chicks acquired high levels of serum antibodies against S. Enteritidis. These results reveal that maternal antibodies in chicks acquired from vaccinated hens through eggs can confer a significant protection against S. Enteritidis infection.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Si
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Shenye
authors.initials: S
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Wanjiang
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China. Electronic address: siguo_liu@hvri.ac.cn.
authors.forename: Siguo
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China. Electronic address: ligx@neau.edu.cn.
authors.forename: Guangxing
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D002645:Chickens / Q000276:immunology*; D004530:Egg Yolk / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005612:Freeze Drying; D007112:Immunity, Maternally-Acquired / Q000276:immunology*; D007116:Immunization, Passive / Q000379:methods / Q000662:veterinary*; D010957:Plasmids; D011201:Poultry Diseases / Q000276:immunology / Q000401:mortality / Q000517:prevention & control*; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000401:mortality / Q000517:prevention & control*; D012477:Salmonella enteritidis / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms; D015996:Survival Rate; D014611:Vaccination / Q000379:methods / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines
keywords: Domestic fowl; Ghost vaccine; Maternally-derived antibodies; Salmonella enterica serovar Enteritidis