title: Direct involvement of CD56 in cytokine-induced killer-mediated lysis of CD56+ hematopoietic target cells.
abstract: Cytokine-induced killer (CIK) cells are in-vitro-expanded T lymphocytes that represent a heterogeneous population. A large majority of CIK cells are CD3(+)CD56(+), and this population has been shown to confer a cytotoxic effect against tumor targets. The scope of this work was to study whether CD56 has a direct role in CIK-mediated cytotoxicity. Blocking of CD56 with the anti-CD56 monoclonal antibody GPR165 significantly reduced CIK-mediated lysis of three CD56(+) hematopoietic tumor cell lines (AML-NS8, NB4, and KCL22), whereas no effect was observed on three CD56(-) hematopoietic tumor cell lines (K562, REH, and MOLT-4). Knockdown of CD56 in CIK cells by short interfering RNA made the cells less cytotoxic against a CD56(+) target, and knockdown of CD56 in target cells with lentiviral short hairpin RNA significantly altered their susceptibility to CIK-mediated lysis. Our data suggest that homophilic interaction between CD56 molecules may occur in tumor-cell recognition, leading to CIK-mediated cell death.
authors.affiliation: USS Centre of Cellular Therapy "G.Lanzani", USC Hematology and Bone Marrow Transplantation Unit, A.O. Papa Giovanni XXIII, Bergamo, Italy.
authors.forename: Rut
authors.initials: R
authors.lastname: Valgardsdottir
authors.affiliation: USS Centre of Cellular Therapy "G.Lanzani", USC Hematology and Bone Marrow Transplantation Unit, A.O. Papa Giovanni XXIII, Bergamo, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Capitanio
authors.affiliation: Nerviano Medical Sciences, Nerviano, Italy.
authors.forename: Gemma
authors.initials: G
authors.lastname: Texido
authors.affiliation: IRCCS AOU San Martino - IST, Genoa, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Pende
authors.affiliation: Istituto Giannina Gaslini, Genoa, Italy; Dipartimento di Medicina Sperimentale e Centro di Eccellenza per la Ricerca Biomedica, Università di Genova, Genoa, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Cantoni
authors.affiliation: Nerviano Medical Sciences, Nerviano, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Pesenti
authors.affiliation: USS Centre of Cellular Therapy "G.Lanzani", USC Hematology and Bone Marrow Transplantation Unit, A.O. Papa Giovanni XXIII, Bergamo, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Rambaldi
authors.affiliation: USS Centre of Cellular Therapy "G.Lanzani", USC Hematology and Bone Marrow Transplantation Unit, A.O. Papa Giovanni XXIII, Bergamo, Italy.
authors.forename: Josée
authors.initials: J
authors.lastname: Golay
authors.affiliation: USS Centre of Cellular Therapy "G.Lanzani", USC Hematology and Bone Marrow Transplantation Unit, A.O. Papa Giovanni XXIII, Bergamo, Italy. Electronic address: mintrona@hpg23.it.
authors.forename: Martino
authors.initials: M
authors.lastname: Introna
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology; D019002:CD56 Antigen / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D055612:Cytokine-Induced Killer Cells / Q000502:physiology*; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic / Q000502:physiology; D018274:Electroporation; D055785:Gene Knockdown Techniques; D005822:Genetic Vectors / Q000235:genetics; D006412:Hematopoietic Stem Cells; D006801:Humans; D007938:Leukemia / Q000473:pathology; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D034741:RNA, Small Interfering / Q000494:pharmacology; D013329:Structure-Activity Relationship
chemical_list: D000911:Antibodies, Monoclonal; D019002:CD56 Antigen; C522918:NCAM1 protein, human; D020033:Protein Isoforms; D034741:RNA, Small Interfering