title: Down-regulated expression of NPM1 in IMS-M2 cell line by (-)-epigallocatechin-3-gallate.
abstract: OBJECTIVE
  To investigate the inhibited effect of epigallocatechin-3-gallate (EGCG) on the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations.
  
  
  METHODS
  Cell proliferation assay was performed to test the effects of EGCG on cell growth of IMS-M2 cells harboring the NPM1 mutations. Western blot analysis were performed to test the protein expression of NPM1, AKT, those associated with apoptosis.
  
  
  RESULTS
  EGCG can down-regulate the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations. Moreover, EGCG also suppressed the cell proliferation and induced apoptosis in IMS-M2 cells.
  
  
  CONCLUSIONS
  The results suggested that EGCG could be considered as a reagent for treatment of AML patients with NPM1 mutations.
authors.affiliation: Department of Molecular Cytogenetics, Hematology and Blood Transfusion Hospital in Ho Chi Minh City, Ho Chi Minh, Vietnam.
authors.forename: Hoang Thanh
authors.initials: HT
authors.lastname: Chi
authors.affiliation: Department of Medical Genome Sciences, Graduate School of Frontier Sciences, the University of Tokyo, Tokyo, Japan.
authors.forename: Bui Thi Kim
authors.initials: BT
authors.lastname: Ly
authors.affiliation: Center for Molecular Biomedicine, The University of Medicine and Pharmacy-Ho Chi Minh City, Ho Chi Minh, Vietnam.
authors.forename: Hoang Anh
authors.initials: HA
authors.lastname: Vu
authors.affiliation: Basic nursing science, The Japanese Red Cross College of Nursing Japan, Tokyo, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Department of Molecular Cytogenetics, Hematology and Blood Transfusion Hospital in Ho Chi Minh City, Ho Chi Minh, Vietnam.
authors.forename: Phu Chi
authors.initials: PC
authors.lastname: Dung
authors.affiliation: Department of Molecular Cytogenetics, Hematology and Blood Transfusion Hospital in Ho Chi Minh City, Ho Chi Minh, Vietnam ; Center for Molecular Biomedicine, The University of Medicine and Pharmacy-Ho Chi Minh City, Ho Chi Minh, Vietnam.
authors.forename: Phan Thi
authors.initials: PT
authors.lastname: Xinh
keywords: Apoptosis; EGCG; IMS-M2; NPM1