title: Diagnostic and prognostic value of microRNA-21 in colorectal cancer: an original study and individual participant data meta-analysis.
abstract: BACKGROUND
  We aimed to systematically summarize the diagnostic and prognostic value of circulating/tissue miR21 in patients with colorectal cancer.
  
  
  METHODS
  An original study was conducted to explore the potential value of circulating miR21 in colorectal cancer diagnosis and tissue miR21 in colorectal cancer prognosis. PUBMED and EMBASE were searched (to August, 2013) to identify eligible studies. To explore the diagnostic performance of circulating miR21, meta-analysis methods were used to pool sensitivity, specificity, positive and negative likelihood ratio, diagnostic OR and to construct a summary ROC curve. For prognostic meta-analysis, study-specific HRs of tissue miR21 for survival were summarized. Subgroup and sensitivity analyses were applied to explore heterogeneity.
  
  
  RESULTS
  Finally, 14 studies (including our study) were included in the meta-analyses. The pooled sensitivity, specificity, and AUC of circulating miR21 were 0.76 [95% confidence interval (CI), 0.59-0.88], 0.81 (95% CI, 0.76-0.85), and 0.81 (95% CI, 0.78-0.85) in diagnosing colorectal cancer. Patients with higher expression of tissue miR21 had significant inferior overall survival (OS; pooled HR, 1.56; 95% CI, 1.16-2.11) and disease-free survival (DFS; pooled HR, 1.35; 95% CI, 1.08-1.69). The individual participant data (IPD) meta-analysis demonstrated that tissue miR21 level was independently associated with worse colorectal cancer OS (HR, 1.69; 95% CI, 1.07-2.67; P = 0.023), whereas this association seems to be confined to males (P = 0.007) but not for females (P = 0.845).
  
  
  CONCLUSIONS
  Circulating miR21 level has potential value for colorectal cancer early detection, whereas high tissue miR21 level is associated with adverse colorectal cancer prognosis.
  
  
  CONCLUSIONS
  miR21 is a promising biomarker for early detection and prognosis of colorectal cancer. Cancer Epidemiol Biomarkers Prev; 23(12); 2783-92. ©2014 AACR.
authors.affiliation: Zhejiang University School of Medicine, Hangzhou, China. Department of Pathology, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Honghe
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Zhejiang University School of Medicine, Hangzhou, China. Department of Gastroenterology, Second Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou, China.
authors.forename: Peiwei
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Surgical Oncology, Zhejiang Cancer Hospital, Hangzhou, China.
authors.forename: Haixing
authors.initials: H
authors.lastname: Ju
authors.affiliation: Department of Biochemistry, Faculty of Medicine in Pilsen, Charles University in Prague, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Pesta
authors.affiliation: Department of Biochemistry, Faculty of Medicine in Pilsen, Charles University in Prague, Czech Republic.
authors.forename: Vlastimil
authors.initials: V
authors.lastname: Kulda
authors.affiliation: Department of Clinical Laboratory, Huzhou Central Hospital, Huzhou, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Jin
authors.affiliation: Anorectal Department, Second Affiliated Hospital, Wenzhou Medical University, WenZhou, China.
authors.forename: Mao
authors.initials: M
authors.lastname: Cai
authors.affiliation: Anorectal Department, Second Affiliated Hospital, Wenzhou Medical University, WenZhou, China.
authors.forename: Changbao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Han
authors.initials: H
authors.lastname: Wu
authors.affiliation: Zhejiang University School of Medicine, Hangzhou, China. Department of Epidemiology and Health Statistics, Zhejiang University School of Public Health, Hangzhou, China.
authors.forename: Jinming
authors.initials: J
authors.lastname: Xu
authors.affiliation: Zhejiang University School of Medicine, Hangzhou, China. Department of Epidemiology and Health Statistics, Zhejiang University School of Public Health, Hangzhou, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Ye
authors.affiliation: Zhejiang University School of Medicine, Hangzhou, China. Department of Pathology, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Guanglin
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Zhejiang University School of Medicine, Hangzhou, China. Department of Pathology, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Enping
authors.initials: E
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou, China.
authors.forename: Jianting
authors.initials: J
authors.lastname: Cai
authors.affiliation: Zhejiang University School of Medicine, Hangzhou, China. Department of Pathology, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Maode
authors.initials: M
authors.lastname: Lai
authors.affiliation: Zhejiang University School of Medicine, Hangzhou, China. Department of Epidemiology and Health Statistics, Zhejiang University School of Public Health, Hangzhou, China.
authors.forename: Dajing
authors.initials: D
authors.lastname: Xia
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. Department of Biomedicine, College of Biotechnology, Zhejiang Agriculture and Forestry University, Hangzhou, China. georgewuer@126.com gastate@zju.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Zhejiang University School of Medicine, Hangzhou, China. Department of Epidemiology and Health Statistics, Zhejiang University School of Public Health, Hangzhou, China. georgewuer@126.com gastate@zju.edu.cn.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Wu
mesh_terms: D014408:Biomarkers, Tumor; D015179:Colorectal Neoplasms / Q000175:diagnosis*; D055088:Early Detection of Cancer; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs