title: Enhancing cubosome functionality by coating with a single layer of poly-ε-lysine.
abstract: We report the preparation and characterization of monoolein cubosomes that can be easily surface modified through adsorption of a single layer of cationic poly-ε-lysine. Poly-ε-lysine coated cubosomes show remarkable stability in serum solution, are nontoxic and, are readily internalized by HeLa cells. The poly-ε-lysine coating provides chemical handles for further bioconjugation of the cubosome surface. We also demonstrate that the initial release rate of a hydrophilic drug, Naproxen sodium, from the cubosomes is retarded with just a single layer of polymer. Interestingly, cubosomes loaded with Naproxen sodium, recently shown to have anticancer properties, cause more apoptosis in HeLa cells when compared to free unencapsulated drug.
authors.affiliation: Division of Polymer Science and Engineering, National Chemical Laboratory , Dr. Homi Bhabha Road, Pune-411008, India.
authors.forename: Sonal
authors.initials: S
authors.lastname: Deshpande
authors.forename: Edakkal
authors.initials: E
authors.lastname: Venugopal
authors.forename: Shobha
authors.initials: S
authors.lastname: Ramagiri
authors.forename: Jayesh R
authors.initials: JR
authors.lastname: Bellare
authors.forename: Guruswamy
authors.initials: G
authors.lastname: Kumaraswamy
authors.forename: Neetu
authors.initials: N
authors.lastname: Singh
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D016503:Drug Delivery Systems; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D009288:Naproxen / Q000494:pharmacology; D011107:Polylysine / Q000737:chemistry*; D053838:Scattering, Small Angle; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D020099:Coated Materials, Biocompatible; D011107:Polylysine; D009288:Naproxen
keywords: bioconjugation; cubosomes; drug delivery vehicle; dual loading; theranostics