title: Characterization of microsatellites for the endangered Ruta oreojasme (Rutaceae) and cross-amplification in related species.
abstract: OBJECTIVE
  Ruta oreojasme is an endangered species endemic to Gran Canaria (Canary Islands, Spain), where it occurs in small populations with disjunct distribution. Nothing is known about the genetic structure of these populations. •
  
  
  RESULTS
  Using a microsatellite-enriched library method, 10 microsatellite markers have been developed from R. oreojasme, all of which showed polymorphism. The transferability of the 10 markers was tested in two other Canarian endemic species, R. microcarpa and R. pinnata, as well as in the widespread species R. montana. •
  
  
  CONCLUSIONS
  Our results demonstrate the value of these newly developed microsatellite markers to investigate the genetic structure in R. oreojasme and show their potential applicability for population genetic studies in other Ruta species.
authors.affiliation: Institute of Systematic Botany, University of Zurich, Zollikerstrase 107, 8008 Zurich, Switzerland.
authors.forename: Marilena
authors.initials: M
authors.lastname: Meloni
authors.affiliation: Department of Environmental Systems Science, ETH Zurich, Universitätstrasse 6, 8006 Zurich, Switzerland.
authors.forename: Andrea
authors.initials: A
authors.lastname: Reid
authors.affiliation: Institute of Systematic Botany, University of Zurich, Zollikerstrase 107, 8008 Zurich, Switzerland.
authors.forename: Elena
authors.initials: E
authors.lastname: Conti
keywords: Ruta oreojasme; Rutaceae; genetic diversity; microsatellites