title: Design of a ProDer f 1 vaccine delivered by the MHC class II pathway of antigen presentation and analysis of the effectiveness for specific immunotherapy.
abstract: Dermatophagoides farinae (Der f 1) is one of leading cause for allergic asthma, and allergen-specific immunotherapy (SIT) is currently recognized as the only etiological therapy to ameliorate asthmatic symptom. The current study was designed on the major histocompatibility complex (MHC) class II pathway, invariant chain (Ii)-segment hybrids as vaccine basis to explore the efficacy of Der f 1 hybrid vaccine by virtue of Ii as carrier in enhancing the protective immune response to asthma. Initially, we engineered a fused molecule, DCP-IhC-ProDer f 1, to deliver ProDer f 1 antigen via specific dendritic cell-targeting peptides to dendritic cells (DCs). Then the DCP-IhC-ProDer f 1 was immunized to the asthmatic models of murine induced by ProDer f 1 allergen. The findings showed that the cytokine repertoire in the murine model was shifted after SIT, including stronger secretion of IFN-γ and IL-10, and a decreased production of IL-4 and IL-17. ELISA determination revealed that the hybrid displayed weak IgE and IgG1 reactivities, and IgG2a levels were elevated. Furthermore, DCP-IhC-ProDer f 1 treatment inhibited inflammatory cell infiltration in the lung tissues. Our results suggest that the DCP-Ihc-ProDer f 1 may be used as a candidate SIT against asthma.
authors.affiliation: Department of Medical Parasitology, Wannan Medical University Wuhu 241002, Anhui, China ; Department of Clinical Laboratory, Affiliated Nanjing Jiangbei Hospital, Medical College of Southeast University Nanjing 210048, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Medical Parasitology, Wannan Medical University Wuhu 241002, Anhui, China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Medical Parasitology, Wannan Medical University Wuhu 241002, Anhui, China.
authors.forename: Chaopin
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D039741:Antigens, Dermatophagoides / Q000276:immunology* / Q000627:therapeutic use; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology* / Q000627:therapeutic use; D060829:Arthropod Proteins / Q000276:immunology* / Q000627:therapeutic use; D001249:Asthma / Q000517:prevention & control*; D015153:Blotting, Western; D003546:Cysteine Endopeptidases / Q000276:immunology* / Q000627:therapeutic use; D003888:Desensitization, Immunologic / Q000379:methods*; D004195:Disease Models, Animal; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000276:immunology* / Q000627:therapeutic use; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000276:immunology / Q000627:therapeutic use; D014612:Vaccines / Q000276:immunology*
chemical_list: D039741:Antigens, Dermatophagoides; D000944:Antigens, Differentiation, B-Lymphocyte; D060829:Arthropod Proteins; D000949:Histocompatibility Antigens Class II; D011994:Recombinant Proteins; D014612:Vaccines; C045084:invariant chain; D003546:Cysteine Endopeptidases; C451159:Dermatophagoides farinae antigen f 1
keywords: Dermatophagoides farinae; dendritic cell; invariant chain; major histocompatibility complex; specific immunotherapy