title: Prognostic value of genetic detection using CEA and MAGE in peritoneal washes with gastric carcinoma after curative resection: result of a 3-year follow-up.
abstract: Peritoneal metastasis is the most frequent cause of death in patients with gastric cancer. Reverse transcriptase-polymerase chain reaction (RT-PCR) assay of peritoneal washes has been used to predict peritoneal metastasis of gastric carcinoma. We applied carcinoembryonic antigen (CEA) and melanoma-associated gene (MAGE) RT-PCR for the detection of peritoneal metastasis of gastric carcinoma after curative surgery and evaluated its clinical significance. Peritoneal washes were obtained from 117 patients with gastric carcinoma. MAGE A1-A6 and CEA RT-PCR were performed, and the results were evaluated according to their clinicopathologic characteristics. Three-year follow-up clinical studies were periodically performed, and disease-free survival rates were retrospectively investigated using the medical records. Among 117 peritoneal fluids, 11 cases (9.4%) revealed MAGE expression and 38 cases (32.5%) revealed CEA expression. When focusing on recurrence rates, RT-PCR-positive had much higher recurrence rates than RT-PCR-negative cases (32.5% vs 5.2%, P < 0.01). Univariate analysis revealed that depth of invasion, lymph node metastasis, tumor node metastasis (TNM) stage, Lauren classification, and MAGE and CEA expressions were independent prognostic factors for recurrence. In a multivariate analysis, MAGE expression and TNM stage were significantly and independently related to recurrence in patients who underwent curative resection. MAGE expression was determined to be the most important prognostic factor for recurrence (hazard ratio: 12.487, P < 0.01). It is feasible to identify free cancer cells in peritoneal lavage by using a MAGE A1-A6 and CEA RT-PCR. MAGE RT-PCR results disclosed significant associations with peritoneal recurrence and proved to be the most important factor for the recurrence rate in patients with gastric carcinoma who had undergone radical resection.
authors.affiliation: Department of Laboratory Medicine (C-HJ); and Department of Surgery (I-HK, H-DC), School of Medicine, Catholic University of Daegu, Daegu, Korea.
authors.forename: Chang-Ho
authors.initials: CH
authors.lastname: Jeon
authors.forename: In-Ho
authors.initials: IH
authors.lastname: Kim
authors.forename: Hyun-Dong
authors.initials: HD
authors.lastname: Chae
mesh_terms: D000368:Aged; D002272:Carcinoembryonic Antigen / Q000096:biosynthesis*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D058950:Melanoma-Specific Antigens / Q000096:biosynthesis*; D008875:Middle Aged; D010533:Peritoneal Lavage; D010534:Peritoneal Neoplasms / Q000175:diagnosis* / Q000556:secondary*; D011379:Prognosis; D013274:Stomach Neoplasms / Q000473:pathology*
chemical_list: D002272:Carcinoembryonic Antigen; D058950:Melanoma-Specific Antigens