title: Trailing or paradoxical growth of Aspergillus flavus exposed to caspofungin is independent of genotype.
abstract: There are limited data on in vitro susceptibility testing of echinocandins against Aspergillus species. The objective of this study was to describe the phenotypes of Aspergillus flavus observed on exposure to caspofungin in vitro and to test whether these phenotypes were associated with A. flavus genotypes. The caspofungin MICs of 37 A. flavus clinical isolates collected from 14 patients with invasive aspergillosis were determined using Etest assays. Caspofungin MICs ranged from 0.012 to 0.064 mg l(-1); the modal MIC was 0.023 mg l(-1) and the MIC₅₀ and MIC₉₀ were 0.032 and 0.064 mg l(-1), respectively. A clear end point was noted in 24 (65 %) isolates, whereas seven (19 %) displayed a trailing effect and six (16 %) showed paradoxical growth when exposed to caspofungin. In these A. flavus isolates, the absence of a significant population structure or genetic differentiation indicated that trailing or paradoxical growth phenotypes were independent of microsatellite genotype.
authors.affiliation: Laboratoire de Biologie Moléculaire Parasitaire et Fongique, Faculté de Médecine de Sfax, Rue Magida Boulila, 3029 Sfax, Tunisia.
authors.forename: Inès
authors.initials: I
authors.lastname: Hadrich
authors.affiliation: Laboratoire de Biologie Moléculaire Parasitaire et Fongique, Faculté de Médecine de Sfax, Rue Magida Boulila, 3029 Sfax, Tunisia.
authors.forename: Sourour
authors.initials: S
authors.lastname: Neji
authors.affiliation: Laboratoire de Biologie Moléculaire Parasitaire et Fongique, Faculté de Médecine de Sfax, Rue Magida Boulila, 3029 Sfax, Tunisia.
authors.forename: Fattouma
authors.initials: F
authors.lastname: Makni
authors.affiliation: Laboratoire de Biologie Moléculaire Parasitaire et Fongique, Faculté de Médecine de Sfax, Rue Magida Boulila, 3029 Sfax, Tunisia.
authors.forename: Ali
authors.initials: A
authors.lastname: Ayadi
authors.affiliation: Service d'Onco-hématologie, CHU Hedi-Chaker, 3029 Sfax, Tunisia.
authors.forename: Moez
authors.initials: M
authors.lastname: Elloumi
authors.affiliation: Aix-Marseille Université, IP-TPT UMR MD3, 13885 Marseille, France.|Parasitology & Mycology, CHU Timone-Adultes, Assistance Publique-Hôpitaux de Marseille, 13005 Marseille, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Ranque
mesh_terms: D000293:Adolescent; D000328:Adult; D000935:Antifungal Agents / Q000494:pharmacology*; D001228:Aspergillosis / Q000382:microbiology*; D001231:Aspergillus flavus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D000077336:Caspofungin; D054714:Echinocandins / Q000494:pharmacology*; D005260:Female; D005838:Genotype; D006801:Humans; D055666:Lipopeptides; D008297:Male; D008826:Microbial Sensitivity Tests; D018895:Microsatellite Repeats; D008875:Middle Aged; D058889:Molecular Typing; D016533:Mycological Typing Techniques; D010641:Phenotype; D055815:Young Adult
chemical_list: D000935:Antifungal Agents; D054714:Echinocandins; D055666:Lipopeptides; D000077336:Caspofungin