title: Conductance catheter measurement and effect of different anesthetics in a rat model of postresuscitation myocardial dysfunction.
abstract: We demonstrate the usefulness of left ventricular pressure-volume (PV) loops generated by the use of conductance catheter measurements and investigate the influence of the type of general anesthesia on postresuscitation myocardial dysfunction in a rat model of cardiac arrest (CA) and subsequent cardiopulmonary resuscitation. A total of 42 Wistar-Han rats were randomized to receive general anesthesia with sevoflurane and resuscitation after CA, general anesthesia with pentobarbital intraperitoneally and resuscitation after CA, or general anesthesia with pentobarbital without CA (sham group). Myocardial function, assessed by analysis of PV loops, was measured continuously and in real-time by using a PV-conductance catheter. Rats were monitored for 3 h after restoration of spontaneous circulation (ROSC). The use of PV-conductance catheters supported objective and reliable evaluation of myocardial function and proved feasible in this rat model of CA. End-diastolic volume increased in rats anesthetized with pentobarbital after ROSC (before CA, 237 ± 45 μL; after ROSC, 402 ± 64 μL). Preloadadjusted maximal power before CA was the same in all groups but decreased in both resuscitated groups. The decrease was less pronounced in rats anesthetized with sevoflurane compared with pentobarbital (11.8 ± 4.9 mW/μL(2) compared with 4.8 ± 1.9 mW/μL(2) at 3 h after ROSC). This finding indicates that the type of general anesthesia influences postresuscitation myocardial dysfunction in this rat model of experimentally induced CA and cardiopulmonary resuscitation. Rats that were anesthetized with sevoflurane exhibited less postresuscitation myocardial dysfunction than did those anesthetized with pentobarbital.
authors.affiliation: Department of Anaesthesiology, University of Heidelberg, Heidelberg, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Knapp
authors.affiliation: Department of Anaesthesiology and Postoperative Intensive Care Medicine, Clinical Center of Osnabrück, Osnabrück, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Teschendorf
authors.affiliation: Department of Internal Medicine III, University of Heidelberg, Heidelberg, Germany.
authors.forename: Eberhard
authors.initials: E
authors.lastname: Scholz
authors.affiliation: Department of Anaesthesiology, University of Heidelberg, Heidelberg, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Roewer
authors.affiliation: Department of Anaesthesiology, University of Heidelberg, Heidelberg, Germany.
authors.forename: Nicolai
authors.initials: N
authors.lastname: Russ
authors.affiliation: Department of Anaesthesiology and Postoperative Intensive Care Medicine, University of Cologne, Köln, Germany.
authors.forename: Bernd W
authors.initials: BW
authors.lastname: Böttiger
authors.affiliation: Department of Anaesthesiology, University of Heidelberg, Heidelberg, Germany.
authors.forename: Erik
authors.initials: E
authors.lastname: Popp
mesh_terms: D000777:Anesthetics / Q000008:administration & dosage*; D000818:Animals; D016887:Cardiopulmonary Resuscitation; D004195:Disease Models, Animal; D006323:Heart Arrest / Q000503:physiopathology*; D006334:Heart Function Tests; D008297:Male; D008738:Methyl Ethers / Q000008:administration & dosage*; D010424:Pentobarbital / Q000008:administration & dosage*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D000077149:Sevoflurane
chemical_list: D000777:Anesthetics; D008738:Methyl Ethers; D000077149:Sevoflurane; D010424:Pentobarbital