title: Baicalin inhibits colistin sulfate-induced apoptosis of PC12 cells.
abstract: Baicalin, a type of flavonoid extracted from the dried root of Scutellaria baicalensis georgi, has been shown to effectively inhibit cell apoptosis. Therefore, we assumed that baicalin would suppress co-listin sulfate-induced neuronal apoptosis. PC12 cells exposed to colistin sulfate (62.5-500 μg/mL) for 24 hours resulted in PC12 cell apoptosis. In addition, caspase-3 activity, lactate dehydrogenase level and free radical content increased in a dose-dependent manner. Subsequently, PC12 cells were pretreated with baicalin (25, 50 and 100 μg/mL), and exposed to 125 μg/mL colistin sulfate. Cell morphology markedly changed, and cell viability increased. Moreover, caspase-3 activity, tate dehydrogenase level and free radical content decreased. Results indicated that baicalin inhi-bited colistin sulfate-induced PC12 cell apoptosis by suppressing free radical injury, and reducing caspase-3 activity and lactate dehydrogenase activity.
authors.affiliation: Institute of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China ; Institute of Animal Husbandry and Veterinary Medicine, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Scientific Research Department, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Lv
authors.affiliation: Institute of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China.
authors.forename: Jichang
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Animal Husbandry and Veterinary Medicine, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institute of Animal Husbandry and Veterinary Medicine, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China.
authors.forename: Tiezhong
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Institute of Animal Husbandry and Veterinary Medicine, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China.
authors.forename: Yingzi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lin
keywords: PC12 cells; apoptosis; baicalin; caspase-3; colistin sulfate; grants-supported paper; lactate dehydrogenase; neural regeneration; neuroregeneration; traditional Chinese medicine