title: Magnetic-encoded fluorescent multifunctional nanospheres for simultaneous multicomponent analysis.
abstract: In this study, magnetic-encoded fluorescent (CdTe/Fe3O4)@SiO2 multifunctional nanospheres were constructed by adjusting the initial concentration of Fe3O4 in a fabrication process based on reverse microemulsion. The resultant multifunctional nanospheres were characterized by transmission electron microscopy, X-ray diffraction measurements, fluorescence spectrophotometry, and vibrating sample magnetometry. They showed good fluorescence properties, gradient magnetic susceptibility (weak, moderate, and strong), and easy biofunctionalization for biomolecules, such as immunoglobulin G (IgG), protein, and antibody. Then the capture efficiency of the (CdTe/Fe3O4)@SiO2 nanospheres were investigated by using the fluorophore-labeled IgG-conjugated nanospheres as a model. Further studies demonstrated the ability of these (CdTe/Fe3O4)@SiO2 multifunctional nanospheres to accomplish sequentially magnetic separation, capture, and fluorescent detection for each corresponding antigen of CA125, AFP, and CEA with a detection limit of 20 KU/L, 10 ng/mL, and 5 ng/mL, respectively, from a mixed sample under a certain external magnetic field within a few minutes. The strategy of combining magnetic-encoding-based separation and fluorescence-based detection proposed in this study shows great potential to achieve easy, rapid, economical, and near-simultaneous multicomponent separation and analysis for a variety of targets such as drugs, biomarkers, pathogens, and so on.
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University) Ministry of Education, College of Pharmaceutical Sciences, Southwest University , Chongqing, 400715, People's Republic of China.
authors.forename: Erqun
authors.initials: E
authors.lastname: Song
authors.forename: Weiye
authors.initials: W
authors.lastname: Han
authors.forename: Jingrong
authors.initials: J
authors.lastname: Li
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Jiang
authors.forename: Dan
authors.initials: D
authors.lastname: Cheng
authors.forename: Yang
authors.initials: Y
authors.lastname: Song
authors.forename: Pu
authors.initials: P
authors.lastname: Zhang
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
mesh_terms: D018394:CA-125 Antigen / Q000032:analysis*; D019187:Cadmium Compounds / Q000737:chemistry; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D004655:Emulsions; D052203:Ferrosoferric Oxide / Q000737:chemistry; D005456:Fluorescent Dyes; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D057230:Limit of Detection; D059346:Magnets; D008565:Membrane Proteins / Q000032:analysis*; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure; D012822:Silicon Dioxide / Q000737:chemistry; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013691:Tellurium / Q000737:chemistry; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: C097353:AFP protein, human; D018394:CA-125 Antigen; D019187:Cadmium Compounds; D002272:Carcinoembryonic Antigen; D004655:Emulsions; D005456:Fluorescent Dyes; D018796:Immunoconjugates; D007074:Immunoglobulin G; C451759:MUC16 protein, human; D008565:Membrane Proteins; D012996:Solutions; D000509:alpha-Fetoproteins; D012822:Silicon Dioxide; D013691:Tellurium; C028337:cadmium telluride; D052203:Ferrosoferric Oxide