title: Humoral and lung immune responses to Mycobacterium tuberculosis infection in a primate model of protection.
abstract: Recently we reported (Mehra et al., 2013), that lung granulomas fromMycobacterium bovisBacille Calmette-Guérin (BCG)-vaccinated cynomolgus macaques exhibit upon challenge withM. tuberculosisa more balanced expression of α- and β-chemokines, relative to comparable samples from sham-vaccinated animals by comparative transcriptomics. Here, we studied the recruitment of immune cells to blood and lungs inM. tuberculosis-infected macaques as a function of prior BCG-vaccination. Vaccination initially enhanced the levels of both macrophages and lymphocytes in blood. In contrast, significantly more CD4+lymphocytes were later recruited to the lungs of sham-vaccinated animals compared with earlier times/BCG vaccinated animals. BCG-vaccination had a short-lived impact on the anti-M. tuberculosisresponse.M. tuberculosiscontinued to replicate in the lung even in the wake of increased CD4+T cell recruitment to primate lungs, indicating that immune subversive mechanisms are key to its survivalin vivo.
authors.affiliation: Tulane National Primate Research Center, Covington, LA, USA.
authors.forename: Noton K
authors.initials: NK
authors.lastname: Dutta
authors.affiliation: Department of Microbiology & Immunology, Tulane University Health Sciences Center, New Orleans, LA, USA.
authors.forename: James
authors.initials: J
authors.lastname: McLachlan
authors.affiliation: Tulane National Primate Research Center, Covington, LA, USA.|Department of Microbiology & Immunology, Tulane University Health Sciences Center, New Orleans, LA, USA.
authors.forename: Smriti
authors.initials: S
authors.lastname: Mehra
authors.affiliation: Tulane National Primate Research Center, Covington, LA, USA.|Department of Microbiology & Immunology, Tulane University Health Sciences Center, New Orleans, LA, USA.
authors.forename: Deepak
authors.initials: D
authors.lastname: Kaushal
keywords: Animal model; BCG; Granuloma; Lymphocyte; M. tuberculosis; Macrophage