title: Protein micropatterns by PEG grafting on Dewetted PLGA films.
abstract: The ability to control protein and cell positioning on a microscopic scale is crucial in many biomedical applications, such as single cell studies. We have developed and investigated the grafting of poly(ethylene glycol) (PEG) brushes onto poly(d,l-lactide-co-glycolide) (PLGA) thin films, which can be micropatterned by exploiting their spontaneous dewetting on top of polystyrene (PS) films. Dense PEG brushes with excellent protein repellence were achieved on PLGA by using cloud point grafting conditions, and selective adsorption of proteins on the micropatterned substrates was achieved by exploiting the different affinity protein adsorption onto the PEG brushes and the PS holes. PEG-grafted PLGA films showed better resistance against spontaneous degradation in buffer than bare PLGA films, due to passivation by the thin PEG coating. The simplicity of dewetting and subsequent grafting approaches, coupled with the ability to coat and pattern nonplanar substrates give rise to possible applications of PEG-grafted PLGA films in single cell studies and cell cultures for tissue engineering.
authors.affiliation: School of Chemistry, The University of Sydney , Sydney, NSW 2006, Australia.
authors.forename: Manuel
authors.initials: M
authors.lastname: Ghezzi
authors.forename: Stuart C
authors.initials: SC
authors.lastname: Thickett
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Telford
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Easton
authors.forename: Laurence
authors.initials: L
authors.lastname: Meagher
authors.forename: Chiara
authors.initials: C
authors.lastname: Neto
mesh_terms: D000327:Adsorption; D000588:Amines / Q000737:chemistry; D000818:Animals; D002417:Cattle; D006868:Hydrolysis; D019344:Lactic Acid / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000588:Amines; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; D011092:Polyethylene Glycols