title: Molecular tethering effect of C-terminus of amyloid peptide aβ42.
abstract: Amyloid peptides are considered to be the main contributor for the membrane disruption related to the pathogenesis of degenerative diseases. The variation of amino acids at the carboxylic terminus of amyloid peptide has revealed significant effects on the modulation of abnormal assemblies of amyloid peptides. In this work, molecular binding agents were tethered to the C-terminus of β-amyloid peptide 1-42 (Aβ42). The molecular interaction between Aβ42 and molecule tethers was identified at single molecule level by using scanning tunneling microscopy (STM). The mechanistic insight into the feature variation of the self-assembly of Aβ42 peptide caused by molecular tethering at C-terminus was clearly revealed, which could appreciably affect the nucleation of amyloid peptide, thus reducing the membrane disruptions.
authors.affiliation: National Center for Nanoscience and Technology , Beijing 100190, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Niu
authors.forename: Meng
authors.initials: M
authors.lastname: Xu
authors.forename: Qiusen
authors.initials: Q
authors.lastname: Han
authors.forename: Hongyang
authors.initials: H
authors.lastname: Duan
authors.forename: Mingdong
authors.initials: M
authors.lastname: Dong
authors.forename: Flemming
authors.initials: F
authors.lastname: Besenbacher
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Yanlian
authors.initials: Y
authors.lastname: Yang
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism* / Q000633:toxicity; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism* / Q000633:toxicity; D066329:Protein Aggregates / Q000187:drug effects; D011485:Protein Binding; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D011725:Pyridines / Q000378:metabolism / Q000494:pharmacology
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D066329:Protein Aggregates; D011725:Pyridines; C075222:amyloid beta-protein (1-42); C034306:4,4'-bipyridyl
keywords: amyloid peptide; membrane disruption; modulation; molecular tethering effect; peptide aggregation