title: Polysaccharides from Angelica sinensis alleviate neuronal cell injury caused by oxidative stress.
abstract: Angelica sinensis has antioxidative and neuroprotective effects. In the present study, we aimed to determine the neuroprotective effect of polysaccharides isolated from Angelica sinensis. In a preliminary experiment, Angelica sinensis polysaccharides not only protected PC12 neuronal cells from H2O2-induced cytotoxicity, but also reduced apoptosis and intracellular reactive oxygen species levels, and increased the mitochondrial membrane potential induced by H2O2 treatment. In a rat model of local cerebral ischemia, we further demonstrated that Angelica sinensis polysaccharides enhanced the antioxidant activity in cerebral cortical neurons, increased the number of microvessels, and improved blood flow after ischemia. Our findings highlight the protective role of polysaccharides isolated from Angelica sinensis against nerve cell injury and impairment caused by oxidative stress.
authors.affiliation: Department of Rehabilitation Medicine, Zhongnan Hospital and Cerebral Vascular Diseases Research Center, Zhongnan Hospital, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Lei
authors.affiliation: School of Pharmaceutical Sciences, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Rehabilitation Medicine, Zhongnan Hospital and Cerebral Vascular Diseases Research Center, Zhongnan Hospital, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Fang
authors.affiliation: Department of Rehabilitation Medicine, Zhongnan Hospital and Cerebral Vascular Diseases Research Center, Zhongnan Hospital, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: Junbin
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Rehabilitation Medicine, Zhongnan Hospital and Cerebral Vascular Diseases Research Center, Zhongnan Hospital, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Sciences, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Xiao
authors.affiliation: School of Pharmaceutical Sciences, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: School of Pharmaceutical Sciences, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Rehabilitation Medicine, Zhongnan Hospital and Cerebral Vascular Diseases Research Center, Zhongnan Hospital, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: Junjian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Sciences, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: Zebo
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Rehabilitation Medicine, Zhongnan Hospital and Cerebral Vascular Diseases Research Center, Zhongnan Hospital, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: Weijing
authors.initials: W
authors.lastname: Liao
keywords: Angelica sinensis; NSFC grant; antioxidation; apoptosis; cerebral ischemia; microvessels; mitochondrial membrane potential; nerve regeneration; neural regeneration; polysaccharides; reactive oxygen species