title: Improved rat liver decellularization by arterial perfusion under oscillating pressure conditions.
abstract: One approach of regenerative medicine to generate functional hepatic tissue in vitro is decellularization and recellularization, and several protocols for the decellularization of livers of different species have been published. This appears to be the first report on rat liver decellularization by perfusion under oscillating pressure conditions, intending to optimize microperfusion and minimize damage to the ECM. Four decellularization protocols were compared: perfusion via the portal vein (PV) or the hepatic artery (HA), with (+P) or without (-P) oscillating pressure conditions. All rat livers (n = 24) were perfused with 1% Triton X-100 and 1% sodium dodecyl sulphate, each for 90 min with a perfusion rate of 5 ml/min. Perfusion decellularization was observed macroscopically and the decellularized liver matrices were analysed by histology and biochemical analyses (e.g. levels of DNA, glycosaminoglycans and hepatocyte growth factor). Livers decellularized via the hepatic artery and under oscillating pressure showed a more homogeneous decellularization and less remaining DNA, compared with the livers of the other experimental groups. The novel decellularization method described is effective, quick (3 h) and gentle to the extracellular matrix and thus represents an improvement of existing methodology. Copyright © 2014 John Wiley & Sons, Ltd.
authors.affiliation: General, Visceral, and Transplantation Surgery, Charité - Campus Virchow, Berlin, Germany.
authors.forename: Benjamin
authors.identifier: 0000-0002-4205-9890
authors.initials: B
authors.lastname: Struecker
authors.affiliation: General, Visceral, and Transplantation Surgery, Charité - Campus Virchow, Berlin, Germany.
authors.forename: Antje
authors.initials: A
authors.lastname: Butter
authors.affiliation: General, Visceral, and Transplantation Surgery, Charité - Campus Virchow, Berlin, Germany.
authors.forename: Karl
authors.initials: K
authors.lastname: Hillebrandt
authors.affiliation: General, Visceral, and Transplantation Surgery, Charité - Campus Virchow, Berlin, Germany.
authors.forename: Dietrich
authors.initials: D
authors.lastname: Polenz
authors.affiliation: General, Visceral, and Transplantation Surgery, Charité - Campus Virchow, Berlin, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Reutzel-Selke
authors.affiliation: General, Visceral, and Transplantation Surgery, Charité - Campus Virchow, Berlin, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Tang
authors.affiliation: General, Visceral, and Transplantation Surgery, Charité - Campus Virchow, Berlin, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Lippert
authors.affiliation: General, Visceral, and Transplantation Surgery, Charité - Campus Virchow, Berlin, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Leder
authors.affiliation: General, Visceral, and Transplantation Surgery, Charité - Campus Virchow, Berlin, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Rohn
authors.affiliation: General, Visceral, and Transplantation Surgery, Charité - Campus Virchow, Berlin, Germany.
authors.forename: Dominik
authors.initials: D
authors.lastname: Geisel
authors.affiliation: General, Visceral, and Transplantation Surgery, Charité - Campus Virchow, Berlin, Germany.
authors.forename: Timm
authors.initials: T
authors.lastname: Denecke
authors.affiliation: General, Visceral, and Transplantation Surgery, Charité - Campus Virchow, Berlin, Germany.
authors.forename: Khalid
authors.initials: K
authors.lastname: Aliyev
authors.affiliation: General, Visceral, and Transplantation Surgery, Charité - Campus Virchow, Berlin, Germany.
authors.forename: Korinna
authors.initials: K
authors.lastname: Jöhrens
authors.affiliation: General, Visceral, and Transplantation Surgery, Charité - Campus Virchow, Berlin, Germany.
authors.forename: Nathanael
authors.initials: N
authors.lastname: Raschzok
authors.affiliation: General, Visceral, and Transplantation Surgery, Charité - Campus Virchow, Berlin, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Neuhaus
authors.affiliation: General, Visceral, and Transplantation Surgery, Charité - Campus Virchow, Berlin, Germany.
authors.forename: Johann
authors.initials: J
authors.lastname: Pratschke
authors.affiliation: General, Visceral, and Transplantation Surgery, Charité - Campus Virchow, Berlin, Germany.
authors.forename: Igor M
authors.initials: IM
authors.lastname: Sauer
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry; D005109:Extracellular Matrix / Q000378:metabolism; D006025:Glycosaminoglycans / Q000737:chemistry; D017228:Hepatocyte Growth Factor / Q000737:chemistry; D008099:Liver / Q000502:physiology*; D008297:Male; D009991:Oscillometry; D010477:Perfusion; D011312:Pressure; D051381:Rats; D011917:Rats, Inbred Lew; D044968:Regenerative Medicine / Q000379:methods*; D046509:Tissue Culture Techniques / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D006025:Glycosaminoglycans; D017228:Hepatocyte Growth Factor; D004247:DNA
keywords: future liver support; liver engineering; oscillating pressure conditions; rat liver decellularization; rat liver perfusion device; rat liver recellularization