title: Physiological techniques in the study of rapid aldosterone effects.
abstract: Molecular imaging and electrophysiological techniques are powerful tools to analyze the responses stimulated by aldosterone and other hormones in target tissues. Studies with Ussing-type chambers can be used to measure and characterize changes in transepithelial currents resulting from hormone treatment. Confocal imaging techniques can be used in real time or in fixed preparations to evaluate the localization of receptors, signalling intermediates, and transporters.
authors.affiliation: Department of Molecular Medicine, RCSI Education & Research Centre, Royal College of Surgeons in Ireland Hospital, Beaumont, Dublin 9, Ireland.
authors.forename: Yamil R
authors.initials: YR
authors.lastname: Yusef
authors.forename: Warren
authors.initials: W
authors.lastname: Thomas
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Harvey
mesh_terms: D000450:Aldosterone / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D004553:Electric Conductivity; D004847:Epithelial Cells / Q000166:cytology*; D004848:Epithelium / Q000502:physiology*; D004867:Equipment Design; D005455:Fluorescent Antibody Technique / Q000379:methods; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D018408:Patch-Clamp Techniques / Q000295:instrumentation / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D000450:Aldosterone