title: Exposure to chlorine dioxide gas for 4 hours renders Syphacia ova nonviable.
abstract: The purpose of our study was to evaluate the efficacy of chlorine dioxide gas for environmental decontamination of Syphacia spp. ova. We collected Syphacia ova by perianal cellophane tape impression of pinworm-infected mice. Tapes with attached ova were exposed to chlorine dioxide gas for 1, 2, 3, or 4 h. After gas exposure, ova were incubated in hatching medium for 6 h to promote hatching. For controls, tapes with attached ova were maintained at room temperature for 1, 2, 3, and 4 h without exposure to chlorine dioxide gas and similarly incubated in hatch medium for 6 h. Ova viability after incubation was assessed by microscopic examination. Exposure to chlorine dioxide gas for 4 h rendered 100% of Syphacia spp. ova nonviable. Conversely, only 17% of ova on the 4-h control slide were nonviable. Other times of exposure to chlorine dioxide gas resulted in variable effectiveness. These data suggest that exposure to chlorine dioxide gas for at least 4 h is effective for surface decontamination of Syphacia spp. ova.
authors.affiliation: Office of Laboratory Animal Care, College of Veterinary Medicine, University of Tennessee, Knoxville, Tennessee, USA.
authors.forename: Jane A
authors.initials: JA
authors.lastname: Czarra
authors.affiliation: Office of Laboratory Animal Care, College of Veterinary Medicine, University of Tennessee, Knoxville, Tennessee, USA.
authors.forename: Joleen K
authors.initials: JK
authors.lastname: Adams
authors.affiliation: Office of Laboratory Animal Care, College of Veterinary Medicine, University of Tennessee, Knoxville, Tennessee, USA.
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Carter
authors.affiliation: Animal Care Unit, Office of Research, The University of Kansas, Lawrence, Kansas, USA; Department of Pharmacology and Toxicology, The University of Kansas, Lawrence, Kansas, USA.
authors.forename: William A
authors.initials: WA
authors.lastname: Hill
authors.affiliation: Office of Laboratory Animal Care, College of Veterinary Medicine, University of Tennessee, Knoxville, Tennessee, USA.
authors.forename: Patricia N
authors.initials: PN
authors.lastname: Coan
mesh_terms: D000818:Animals; D017606:Chlorine Compounds / Q000494:pharmacology*; D003666:Decontamination; D017229:Enterobiasis / Q000469:parasitology; D051379:Mice; D010063:Ovum / Q000187:drug effects; D010087:Oxides / Q000494:pharmacology*; D010124:Oxyuroidea / Q000187:drug effects* / Q000254:growth & development
chemical_list: D017606:Chlorine Compounds; D010087:Oxides; C025109:chlorine dioxide