title: Pvr expression regulators in equilibrium signal control and maintenance of Drosophila blood progenitors.
abstract: Blood progenitors within the lymph gland, a larval organ that supports hematopoiesis in Drosophila melanogaster, are maintained by integrating signals emanating from niche-like cells and those from differentiating blood cells. We term the signal from differentiating cells the 'equilibrium signal' in order to distinguish it from the 'niche signal'. Earlier we showed that equilibrium signaling utilizes Pvr (the Drosophila PDGF/VEGF receptor), STAT92E, and adenosine deaminase-related growth factor A (ADGF-A) (Mondal et al., 2011). Little is known about how this signal initiates during hematopoietic development. To identify new genes involved in lymph gland blood progenitor maintenance, particularly those involved in equilibrium signaling, we performed a genetic screen that identified bip1 (bric à brac interacting protein 1) and Nucleoporin 98 (Nup98) as additional regulators of the equilibrium signal. We show that the products of these genes along with the Bip1-interacting protein RpS8 (Ribosomal protein S8) are required for the proper expression of Pvr.
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
authors.forename: Bama Charan
authors.initials: BC
authors.lastname: Mondal
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
authors.forename: Jiwon
authors.identifier: http://orcid.org/0000-0003-2409-1130
authors.initials: J
authors.lastname: Shim
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
authors.forename: Cory J
authors.initials: CJ
authors.lastname: Evans
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
authors.forename: Utpal
authors.initials: U
authors.lastname: Banerjee
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D017344:Genes, Insect; D056726:Genetic Association Studies; D005820:Genetic Testing; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D008198:Lymph Nodes / Q000166:cytology; D008954:Models, Biological; D010641:Phenotype; D034622:RNA Interference; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000235:genetics
chemical_list: D029721:Drosophila Proteins; C438368:Pvr protein, Drosophila; D020794:Receptor Protein-Tyrosine Kinases
keywords: D. melanogaster; developmental biology; equilibrium signaling; hematopoiesis; hemocyte; lymph gland; macrophage; niche signaling; stem cells