title: Low-level expression of purine bases in BALB/3T3 cells monitored by ultrasensitive graphene-based glass carbon electrode.
abstract: Using an ultrasensitive chemically reduced graphene oxide and ionic liquid modified glass carbon electrode (RGI-GCE), separated electrochemical signals of adenine and hypoxanthine in both human breast cancer (MCF-7) and mouse embryonic fibroblast (BALB/3T3) cells were observed. For the first time, low-level expression of purine bases in noncancerous BALB/3T3 cells can be electrochemically monitored. The metabolism of purine bases in carcinogen agent-contaminated BALB/3T3 cells was also investigated through the change of electrochemical signals ascribed to different purine bases, which opens a new electrochemical approach to the exploration of a low-level purine mechanism in noncancerous cells.
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi 154007, China.
authors.forename: Guang-Gang
authors.initials: GG
authors.lastname: Gao
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi 154007, China.
authors.forename: Guo-Biao
authors.initials: GB
authors.lastname: Xu
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi 154007, China.
authors.forename: Jin-Lian
authors.initials: JL
authors.lastname: Li
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi 154007, China.
authors.forename: Ji-Wen
authors.initials: JW
authors.lastname: Cui
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi 154007, China. Electronic address: dmwu@jmsu.edu.cn.
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Wu
authors.affiliation: School of Public Health, Jiamusi University, Jiamusi 154007, China. Electronic address: qhbin63@163.com.
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Qiu
mesh_terms: D000225:Adenine / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D041702:BALB 3T3 Cells; D015374:Biosensing Techniques / Q000379:methods; D002244:Carbon / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D005898:Glass / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D019271:Hypoxanthine / Q000032:analysis* / Q000378:metabolism; D061986:MCF-7 Cells; D051379:Mice; D010084:Oxidation-Reduction
chemical_list: D019271:Hypoxanthine; D002244:Carbon; D006108:Graphite; D000225:Adenine
keywords: Cells; Electrochemical; Graphene; Ionic liquid; Purine