title: Spectroscopic study on the formation of DNA-Ag clusters and its application in temperature sensitive vehicles of DOX.
abstract: DNA silver nanoclusters (DNA-AgNCs) with a fluorescence emission at 610 nm were synthesized using a special hairpin DNA sequence (5'-AGCACGTAG-C3AC3AC3GC3A-CTACGTGCT-3'). Spectroscopic data demonstrate that the DNA changed from an i-motif structure containing C-quadruplexes to anti-parallel four strands structure during the formation of DNA-AgNCs. Importantly, the loose and compact four strand structure caused by the melting and hybridization of stem duplex was confirmed by the reversible fluorescence change of DNA-AgNCs in the range of 25-66°C. Herein, DNA-AgNCs were used as temperature sensitive vehicles of drug loading. The drug loading capacity is 1 Doxorubicin (Dox) molecules per CG pairs on stem-duplexes. The loaded Dox can be released by raising temperature with the melt of stem duplex. Moreover, the special DNA sequence makes it sensitive to the HepG-2 cells.
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Zhao
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China. Electronic address: chenqy@ujs.edu.cn.
authors.forename: Qiu-Yun
authors.initials: QY
authors.lastname: Chen
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: Yang
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000737:chemistry*; D004317:Doxorubicin / Q000008:administration & dosage*; D056945:Hep G2 Cells; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy; D012834:Silver / Q000737:chemistry*; D013696:Temperature
chemical_list: D000903:Antibiotics, Antineoplastic; D003692:Delayed-Action Preparations; D012834:Silver; D004317:Doxorubicin; D004247:DNA
keywords: Aptamer; DNA-Ag cluster; Dox; Temperature; Vehicle