title: Comparison of the Simplexa™ Flu A/B & RSV kit (nucleic acid extraction-dependent assay) and the Prodessa ProFlu+™ assay for detecting influenza and respiratory syncytial viruses.
abstract: The relative performance of 2 widely used reverse transcription polymerase chain reaction (RT-PCR) assays, the Focus diagnostics Simplexa™ Flu A/B & RSV kit (nucleic acid extraction-dependent assay) and the Prodessa Proflu+™ assay, was evaluated using 735 prospectively and retrospectively collected nasopharyngeal swab specimens. Overall, the assays showed positive and negative agreements of 100% and 99.7% for influenza A, 98.1% and 99.9% for influenza B, and 99.3% and 99.5% for respiratory syncytial virus. The relative analytical sensitivity of the 2 assays was also similar.
authors.affiliation: University of Rochester Medical Center, Rochester, NY 14642, USA.
authors.forename: Suresh B
authors.initials: SB
authors.lastname: Selvaraju
authors.affiliation: University of Rochester Medical Center, Rochester, NY 14642, USA.
authors.forename: Adrienne V
authors.initials: AV
authors.lastname: Bambach
authors.affiliation: Nationwide Children's Hospital, Columbus, OH 43205, USA.
authors.forename: Amy L
authors.initials: AL
authors.lastname: Leber
authors.affiliation: University of Rochester Medical Center, Rochester, NY 14642, USA.
authors.forename: Maria-Magdalena
authors.initials: MM
authors.lastname: Patru
authors.affiliation: LeBonheur Children's Hospital, Memphis, TN 38103, USA.
authors.forename: Anami
authors.initials: A
authors.lastname: Patel
authors.affiliation: University of Rochester Medical Center, Rochester, NY 14642, USA. Electronic address: marilyn_menegus@urmc.rochester.edu.
authors.forename: Marilyn A
authors.initials: MA
authors.lastname: Menegus
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D009980:Influenza A virus / Q000235:genetics*; D009981:Influenza B virus / Q000235:genetics*; D007251:Influenza, Human / Q000175:diagnosis / Q000821:virology*; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009305:Nasopharynx / Q000821:virology; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis / Q000821:virology*; D012136:Respiratory Syncytial Viruses / Q000235:genetics*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D055815:Young Adult
keywords: FOCUS nucleic acid extraction–dependent assay; Flu and RSV detection; Prodessa Proflu assay; Simplexa