title: Development and characterization of microsatellite markers for Lilium longiflorum (Liliaceae).
abstract: OBJECTIVE
  Ten microsatellite primers were developed to obtain information on genetic variation in Lilium longiflorum, a bulbous species showing high intraspecific genetic differentiation. •
  
  
  RESULTS
  Of 61 microsatellite loci isolated using the dual suppression PCR technique, 10 loci were effective to characterize and estimate genetic variation in two populations of L. longiflorum. The number of alleles at each locus was different between the populations (averages = 3.2 and 10.3 alleles per locus), and the mean observed heterozygosity values were 0.245 and 0.732. •
  
  
  CONCLUSIONS
  Our results demonstrate that there is significant genetic variation between the populations and that the microsatellite markers developed in this study will be useful tools for the investigation of the genetic structure and mating system of natural L. longiflorum populations.
authors.affiliation: Laboratory of Agricultural Ecology, Faculty of Agriculture, Kyushu University, Kasuya, Fukuoka, 811-2307, Japan.
authors.forename: Satomi
authors.initials: S
authors.lastname: Sakazono
authors.affiliation: Laboratory of Horticultural Science, Faculty of Agriculture, Kyushu University, Fukuoka, 812-8581, Japan.
authors.forename: Michikazu
authors.initials: M
authors.lastname: Hiramatsu
authors.affiliation: Graduate School of Life Sciences, Tohoku University, Miyagi, 980-8577, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Watanabe
authors.affiliation: Laboratory of Horticultural Science, Faculty of Agriculture, Kyushu University, Fukuoka, 812-8581, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Okubo
keywords: Liliaceae; Lilium longiflorum; genetic variation; natural population; self-compatibility; self-incompatibility