title: Transcriptomic and proteomic analyses of embryogenic tissues in Picea balfouriana treated with 6-benzylaminopurine.
abstract: The cytokinin 6-benzylaminopurine (6-BAP) influences the embryogenic capacity of the tissues of Picea balfouriana during long subculture (after 3 months). Tissues that proliferate in 3.6 and 5 µM 6-BAP exhibit the highest and lowest embryogenic capacity, respectively, generating 113 ± 6 and 23 ± 3 mature embryos per 100 mg of tissue. In this study, a comparative transcriptomic and proteomic approach was applied to characterize the genes and proteins that are differentially expressed among tissues under the influence of different levels of 6-BAP. A total of 51 375 unigenes and 2617 proteins were obtained after quality filtering. There were 2770 transcripts for proteins found among these unigenes. Gene ontology (GO) analysis of the differentially expressed unigenes and proteins showed that they were involved in cell and binding activity and were enriched in ribosome and glutathione metabolism pathways. Ribosomal proteins, glutathione S-transferase proteins, germin-like proteins and calmodulin-independent protein kinases were up-regulated in the embryogenic tissues with the highest embryogenic ability (treated with 3.6 µM 6-BAP), which was validated via quantitative real-time polymerase chain reaction (qRT-PCR) analysis, and these proteins might serve as molecular markers of embryogenic ability. Data are available via Sequence Read Archive (SRA) and ProteomeXchange with identifier SRP042246 and PXD001022, respectively.
authors.affiliation: State Key Laboratory of Forest Genetics and Tree Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
authors.forename: Qingfen
authors.initials: Q
authors.lastname: Li
authors.forename: Shougong
authors.initials: S
authors.lastname: Zhang
authors.forename: Junhui
authors.initials: J
authors.lastname: Wang
mesh_terms: D001593:Benzyl Compounds; D020869:Gene Expression Profiling; D007210:Indoleacetic Acids / Q000378:metabolism; D007541:Isopentenyladenosine / Q000031:analogs & derivatives / Q000378:metabolism; D007701:Kinetin / Q000494:pharmacology*; D058977:Molecular Sequence Annotation; D028222:Picea / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism; D058439:Plant Somatic Embryogenesis Techniques; D020543:Proteome; D011687:Purines; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D001593:Benzyl Compounds; D007210:Indoleacetic Acids; D020543:Proteome; D011687:Purines; C030737:indoleacetic acid; D007541:Isopentenyladenosine; C009699:zeatin riboside; C480551:benzylaminopurine; D007701:Kinetin