title: Paenibacillus guangzhouensis sp. nov., an Fe(III)- and humus-reducing bacterium from a forest soil.
abstract: A Gram-reaction-variable, rod-shaped, motile, facultatively aerobic and endospore-forming bacterium, designated strain GSS02(T), was isolated from a forest soil. Strain GSS02(T) was capable of reducing humic substances and Fe(III) oxides. Strain GSS02(T) grew optimally at 35 °C, at pH 78 and in the presence of 1% NaCl. The predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C(15:0) and iso-C(16:0) and the polar lipid profile contained mainly phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol, with moderate amounts of two unknown aminophospholipids and a minor amount of one unknown lipid. The DNA G+C content was 53.4 mol%. Comparative 16S rRNA gene sequence analysis showed that strain GSS02(T) was related most closely to Paenibacillus terrigena JCM 21741(T) (98.1% similarity). Mean DNA-DNA relatedness between strain GSS02(T) and P. terrigena JCM 21741(T) was 58.8 ± 0.5%. The phylogenetic, chemotaxonomic and phenotypic results clearly demonstrated that strain GSS02(T) belongs to the genus Paenibacillus and represents a novel species, for which the name Paenibacillus guangzhouensis sp. nov. is proposed. The type strain is GSS02(T) ( =KCTC 33171(T) =CCTCC AB 2013236(T)).
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.|College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, PR China.
authors.forename: Jibing
authors.initials: J
authors.lastname: Li
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Lu
authors.affiliation: College of Orient Science and Technology, Hunan Agricultural University, Changsha 410128, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors.forename: Shungui
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors.forename: Guiqin
authors.initials: G
authors.lastname: Yang
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005290:Ferric Compounds / Q000378:metabolism; D065928:Forests; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D056507:Paenibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D005290:Ferric Compounds; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7