title: Adenosine triphosphate promotes locomotor recovery after spinal cord injury by activating mammalian target of rapamycin pathway in rats.
abstract: The mammalian target of rapamycin (mTOR) pathway plays an important role in neuronal growth, proliferation and differentiation. To better understand the role of mTOR pathway involved in the induction of spinal cord injury, rat models of spinal cord injury were established by modified Allen's stall method and interfered for 7 days by intraperitoneal administration of mTOR activator adenosine triphosphate and mTOR kinase inhibitor rapamycin. At 1-4 weeks after spinal cord injury induction, the Basso, Beattie and Bresnahan locomotor rating scale was used to evaluate rat locomotor function, and immunohistochemical staining and western blot analysis were used to detect the expression of nestin (neural stem cell marker), neuronal nuclei (neuronal marker), neuron specific enolase, neurofilament protein 200 (axonal marker), glial fibrillary acidic protein (astrocyte marker), Akt, mTOR and signal transduction and activator of transcription 3 (STAT3). Results showed th+at adenosine triphosphate-mediated Akt/mTOR/STAT3 pathway increased endogenous neural stem cells, induced neurogenesis and axonal growth, inhibited excessive astrogliosis and improved the locomotor function of rats with spinal cord injury.
authors.affiliation: Department of Spine Surgery, First People's Hospital of Qingdao Economic & Technological Development Area, Qingdao 266555, Shandong Province, China ; Second Clinical Medical College, Lanzhou University, Lanzhou 730000, Gansu Province, China.
authors.forename: Zhengang
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Second Clinical Medical College, Lanzhou University, Lanzhou 730000, Gansu Province, China ; Department of Orthopedics, Nanchong Central Hospital, Nanchong 637000, Sichuan Province, China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Hu
authors.affiliation: Second Clinical Medical College, Lanzhou University, Lanzhou 730000, Gansu Province, China ; Department of Spine Surgery, Lanzhou General Hospital of Lanzhou Military Region, Lanzhou 730050, Gansu Province, China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Department of Spine Surgery, Lanzhou General Hospital of Lanzhou Military Region, Lanzhou 730050, Gansu Province, China.
authors.forename: Keming
authors.initials: K
authors.lastname: Chen
authors.affiliation: Department of Information, Qingdao Eighth People's Hospital, Qingdao 266100, Shandong Province, China.
authors.forename: Zhenjuan
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Second Clinical Medical College, Lanzhou University, Lanzhou 730000, Gansu Province, China.
authors.forename: Haiyuan
authors.initials: H
authors.lastname: Yue
authors.affiliation: Second Clinical Medical College, Lanzhou University, Lanzhou 730000, Gansu Province, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
keywords: adenosine triphosphate; mammalian target of rapamycin pathway; neural regeneration; neuroregeneration; photographs-containing paper; rapamycin; serine/threonine-specific protein kinase; signal pathway; signal transduction and activator of transcription 3; spinal cord injury