title: Anti-osteopontin monoclonal antibody prevents ovariectomy-induced osteoporosis in mice by promotion of osteoclast apoptosis.
abstract: Osteopontin (OPN) is abundant in mineralized tissues and has long been implicated in bone remodeling. However, the therapeutic effect of targeting OPN in bone loss diseases and the underlying molecular mechanism remain largely unknown. Here, we reported that anti-OPN mAb (23C3) could protect against ovariectomy-induced osteoporosis in mice, demonstrated by microcomputed tomography analysis and histopathology evaluation. In vitro assay showed that 23C3 mAb reduced osteoclasts (OCs)-mediated bone resorption through promotion of mature OC apoptosis. Thus, the study has important implications for understanding the role of OPN in OC bone resorption and survival, and OPN antagonists may have therapeutic potential for osteoporosis and other osteopenic diseases.
authors.affiliation: International Joint Cancer Institute, The Second Military Medical University, 800 Xiang Yin Road, Shanghai 200433, People's Republic of China; PLA General Hospital Cancer Center & PLA Cancer Research Institute, PLA Postgraduate School of Medicine, 28 Fuxing Road, Beijing, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: International Joint Cancer Institute, The Second Military Medical University, 800 Xiang Yin Road, Shanghai 200433, People's Republic of China; PLA General Hospital Cancer Center & PLA Cancer Research Institute, PLA Postgraduate School of Medicine, 28 Fuxing Road, Beijing, People's Republic of China; National Engineering Research Center for Antibody Medicine and Shanghai Key Lab. of Cell Engineering and Antibody, 399 Libing Road, Shanghai 201203, People's Republic of China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Dai
authors.affiliation: International Joint Cancer Institute, The Second Military Medical University, 800 Xiang Yin Road, Shanghai 200433, People's Republic of China.
authors.forename: Huaqing
authors.initials: H
authors.lastname: Wang
authors.affiliation: International Joint Cancer Institute, The Second Military Medical University, 800 Xiang Yin Road, Shanghai 200433, People's Republic of China; PLA General Hospital Cancer Center & PLA Cancer Research Institute, PLA Postgraduate School of Medicine, 28 Fuxing Road, Beijing, People's Republic of China.
authors.forename: Huafeng
authors.initials: H
authors.lastname: Wei
authors.affiliation: International Joint Cancer Institute, The Second Military Medical University, 800 Xiang Yin Road, Shanghai 200433, People's Republic of China; National Engineering Research Center for Antibody Medicine and Shanghai Key Lab. of Cell Engineering and Antibody, 399 Libing Road, Shanghai 201203, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.affiliation: International Joint Cancer Institute, The Second Military Medical University, 800 Xiang Yin Road, Shanghai 200433, People's Republic of China; PLA General Hospital Cancer Center & PLA Cancer Research Institute, PLA Postgraduate School of Medicine, 28 Fuxing Road, Beijing, People's Republic of China; National Engineering Research Center for Antibody Medicine and Shanghai Key Lab. of Cell Engineering and Antibody, 399 Libing Road, Shanghai 201203, People's Republic of China. Electronic address: yguo_smmu@163.com.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Guo
authors.affiliation: International Joint Cancer Institute, The Second Military Medical University, 800 Xiang Yin Road, Shanghai 200433, People's Republic of China; PLA General Hospital Cancer Center & PLA Cancer Research Institute, PLA Postgraduate School of Medicine, 28 Fuxing Road, Beijing, People's Republic of China; National Engineering Research Center for Antibody Medicine and Shanghai Key Lab. of Cell Engineering and Antibody, 399 Libing Road, Shanghai 201203, People's Republic of China. Electronic address: kexingfan@126.com.
authors.forename: Kexing
authors.initials: K
authors.lastname: Fan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D015519:Bone Density / Q000187:drug effects; D001862:Bone Resorption / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000209:etiology / Q000235:genetics; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression; D007274:Injections, Intraperitoneal; D051379:Mice; D008810:Mice, Inbred C57BL; D010010:Osteoclasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D053495:Osteopontin / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D010024:Osteoporosis / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000209:etiology / Q000235:genetics; D010052:Ovariectomy / Q000009:adverse effects*; D055114:X-Ray Microtomography
chemical_list: D000911:Antibodies, Monoclonal; C506896:Spp1 protein, mouse; D053495:Osteopontin
keywords: Bone resorption; Osteoclast survival; Osteopontin; Osteoporosis