title: Seasonal dynamics of water bloom-forming Microcystis morphospecies and the associated extracellular microcystin concentrations in large, shallow, eutrophic Dianchi Lake.
abstract: The increasing occurrence of Microcystis blooms is of great concern to public health and ecosystem due to the potential hepatotoxic microcystins (MCs) produced by these colonial cyanobacteria. In order to interpret the relationships between variations of Microcystis morphospecies and extracellular MC concentrations, the seasonal dynamics of phytoplankton community composition, MC concentrations, and environmental parameters were monitored monthly from August, 2009 to July, 2010. The results indicated that Microcystis dominated total phytoplankton abundance from May to December (96%-99% of total biovolume), with toxic Microcystis viridis and non-toxic Microcystis wesenbergii dominating after July (constituting 65%-95% of the Microcystis population), followed by M. viridis as the sole dominant species from November to January (49%-93%). Correlation analysis revealed that water temperature and nutrient were the most important variables accounting for the occurrence of M. wesenbergii, while the dominance of M. viridis was related with nitrite and nitrate. The relatively low content of MCs was explained by the association with a large proportion of M. viridis and M. wesenbergii, small colony size of Microcystis populations, and low water temperature, pH and dissolved oxygen. The extracellular MC (mean of 0.5±0.2μg/L) of water samples analyzed by enzyme-linked immunosorbent assay (ELISA) demonstrated the low concentrations of MC in Dianchi Lake which implied the low potential risk for human health in the basin. The survey provides the first whole lake study of the occurrence and seasonal variability of Microcystis population and extracellular MCs that are of particular interest for water quality monitoring and management.
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. Electronic address: ljf19841113@163.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Nanqin
authors.initials: N
authors.lastname: Gan
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Ma
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Shan
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Bangding
authors.initials: B
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. Electronic address: lrsong@ihb.ac.cn.
authors.forename: Lirong
authors.initials: L
authors.lastname: Song
mesh_terms: D002681:China; D002734:Chlorophyll / Q000032:analysis; D000077194:Chlorophyll A; D057097:Harmful Algal Bloom; D060106:Lakes / Q000737:chemistry; D052998:Microcystins / Q000032:analysis* / Q000633:toxicity; D046931:Microcystis; D010839:Phytoplankton; D012621:Seasons
chemical_list: D052998:Microcystins; D002734:Chlorophyll; C078588:microcystin; D000077194:Chlorophyll A
keywords: Cyanobacterial blooms; Dianchi Lake; Microcystis succession; Morphospecies; Toxicity