title: Changes in urinary metabolic profile after oral administration of curcuma extract in rats.
abstract: The diffusion of phytochemicals in health promoting products is growing, but studies related to their effects on healthy subjects are still lacking despite the large consumption of natural products as nutraceuticals or food supplements. In many cases, research supports the in vitro antioxidant activity of phytochemicals, but the health claims attributed to the final marketed nutraceutical products have dubious scientific foundation. Also, studies focussed on the definition of their biological targets and mechanisms of action can be useful to assess their efficacy and safety. In this study, the effect of oral administration of 80mg/kg of Curcuma longa Linn. extract to 12 healthy rats over 25 days was evaluated by monitoring the changes of urinary composition. 24-h urine was collected during the animal experiment and the composition was analyzed by (1)H NMR and HPLC-MS. The two datasets were studied individually through a metabolomic approach and the multivariate analysis revealed significant differences between the control and the treated group. Curcumin levels were also measured in 24-h urine samples by HPLC-MS. Both the (1)H NMR and the HPLC-MS dataset showed that the administration of 80mg/kg of Curcuma longa extract to healthy animals induces changes in urinary composition. Decreased allantoin urinary levels can be considered a partial demonstration of the in vivo effect of curcumin on oxidative stress in a healthy animal model.
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35131 Padova, Italy. Electronic address: stefano.dallacqua@unipd.it.
authors.forename: Stefano
authors.initials: S
authors.lastname: Dall'Acqua
authors.affiliation: S-IN Soluzioni Informatiche, Via Ferrari 14, 36100 Vicenza, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Stocchero
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35131 Padova, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Clauser
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35131 Padova, Italy.
authors.forename: Irene
authors.initials: I
authors.lastname: Boschiero
authors.affiliation: Department of Chemical Sciences, University of Padova, Via Marzolo 1, 35131 Padova, Italy.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Ndoum
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35131 Padova, Italy.
authors.forename: Mariano
authors.initials: M
authors.lastname: Schiavon
authors.affiliation: Department of Chemical Sciences, University of Padova, Via Marzolo 1, 35131 Padova, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Mammi
authors.affiliation: Department of Chemical Sciences, University of Padova, Via Marzolo 1, 35131 Padova, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Schievano
mesh_terms: D000284:Administration, Oral; D000481:Allantoin / Q000652:urine*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000493:pharmacokinetics; D015415:Biomarkers / Q000652:urine; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D030024:Curcuma; D003474:Curcumin / Q000008:administration & dosage* / Q000493:pharmacokinetics; D005260:Female; D008297:Male; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods; D015999:Multivariate Analysis; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000652:urine*; D010946:Plants, Medicinal; D066244:Proton Magnetic Resonance Spectroscopy; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D010936:Plant Extracts; D000481:Allantoin; D003474:Curcumin
keywords: ASCA; Curcumin; HPLC-MS; Metabolomic; NMR