title: Genome-wide identification and expression analysis of microRNA involved in small cell lung cancer via deep sequencing.
abstract: Small cell lung cancer is a major cause of mortality worldwide. microRNAs (miRNAs) are involved in various biological processes through regulating gene expression. In the present study, to identify the miRNAs involved in human small cell lung cancer at the genome-wide level, Solexa sequencing was employed to sequence two small RNA (sRNA) libraries from small cell lung cancer tissues (LC sRNA library) and the corresponding normal tissues (NT sRNA library). Deep sequencing of the two sRNA libraries identified a number of conserved miRNAs and differential expression analysis of these miRNAs revealed 81 miRNAs differentially expressed in small cell lung cancer, of which more than half were downregulated. The expression trends determined by sequencing were validated by reverse transcription-quantitative polymerase chain reaction analysis. The annotations for the targets of these miRNAs were predicted. This study provides valuable information for understanding the regulatory mechanisms of miRNAs involved in human small cell lung cancer.
authors.affiliation: Department of Respiratory Medicine, the Second Affiliated Hospital of Harbin Medical University, Harbin 150081, P.R. China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Yan
authors.affiliation: Department of Neurology, the Second Affiliated Hospital of Harbin Medical University, Harbin Medical University, Harbin 150081, P.R. China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Shi
authors.affiliation: Department of Respiratory Medicine, the Second Affiliated Hospital of Harbin Medical University, Harbin Medical University, Harbin 150081, P.R. China.
authors.forename: Qiushi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Occupational Health, Public Health College, Harbin Medical University, Harbin 150081, P.R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Respiratory Medicine, the Second Affiliated Hospital of Harbin Medical University, Harbin 150081, P.R. China.
authors.forename: Yaxin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Respiratory Medicine, the Second Affiliated Hospital of Harbin Medical University, Harbin 150081, P.R. China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, the Second Affiliated Hospital of Harbin Medical University, Harbin 150081, P.R. China.
authors.forename: Yuandi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Respiratory Medicine, the Second Affiliated Hospital of Harbin Medical University, Harbin 150081, P.R. China.
authors.forename: Fuzhen
authors.initials: F
authors.lastname: Lv
authors.affiliation: Department of Respiratory Medicine, the Second Affiliated Hospital of Harbin Medical University, Harbin 150081, P.R. China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Shao
mesh_terms: D001483:Base Sequence; D016022:Case-Control Studies; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D015894:Genome, Human; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D058977:Molecular Sequence Annotation; D017423:Sequence Analysis, RNA; D055752:Small Cell Lung Carcinoma / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D035683:MicroRNAs