title: Theoretical and experimental study on lipophilicity and wound healing activity of ginger compounds.
abstract: OBJECTIVE
  To correlate the chromatographic and computational method to calculate lipophilicity of selected ginger compounds and to observe the effects of log P on wound healing.
  
  
  METHODS
  Mixtures of acetonitrile and water with acetonitrile content between 95% and 50% v/v in 5% increments were kept separately in 10 different chromatographic chambers, saturated with solvent for 2 h. Spots were observed under UV light at λ=254 nm p-anisaldehyde used as a spraying reagent. Theoretical calculation was done using the Alogps 2.1 online program at www.vcclab.org/lab/alogps. For percentage wound contraction, five groups of animal (mice) (25-30 g) of either sex were selected. Wound were created on dorsal surface of animals using toothed forceps, scalpel and pointed scissors. The wound areas were calculated using vernier caliper. After making wound mice were orally administered 35 mg/kg 6-shogoal, 6-gingerol, 8-gingerol and 10-gingerol respectively. Group E as the control group received tap water.
  
  
  RESULTS
  The lipophilicity values determined in thin layer chromatography were correlated with the theoretically calculated various log P by linear regression analysis. Significant correlations were found between log P values calculated by software program and the experimental reversed-phase thin-layer chromatography data. Order of wound healing property of ginger compounds is directly dependent on lipophilicity i.e. more lipophilic compound has highest activity.
  
  
  CONCLUSIONS
  Experimentally determined lipophilicity (R MO) values were correlated with log P determined by software's and found satisfactory. Lipophilicity (R MO) is a useful parameter for the determination and prediction of biological activity of ginger compounds.
authors.affiliation: Department of Pharmaceutical Chemistry, College of Pharmacy, Salman Bin AbdulAziz University, P.O. Box 173, Al-Kharj 11942, Kingdom of Saudi Arabia.
authors.forename: Mohammed Afroz
authors.initials: MA
authors.lastname: Bakht
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Mohammed F
authors.initials: MF
authors.lastname: Alajmi
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Perwez
authors.initials: P
authors.lastname: Alam
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, Salman Bin AbdulAziz University, P.O. Box 173, Al-Kharj 11942, Kingdom of Saudi Arabia.
authors.forename: Aftab
authors.initials: A
authors.lastname: Alam
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, Salman Bin AbdulAziz University, P.O. Box 173, Al-Kharj 11942, Kingdom of Saudi Arabia.
authors.forename: Prawez
authors.initials: P
authors.lastname: Alam
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, Salman Bin AbdulAziz University, P.O. Box 173, Al-Kharj 11942, Kingdom of Saudi Arabia.
authors.forename: Tariq Mohammed
authors.initials: TM
authors.lastname: Aljarba
keywords: Calculated partition coefficient; RM0; RP-TLC; Wound healing; lipophilicity