title: PD-L1 is increased in the spinal cord and infiltrating lymphocytes in experimental allergic encephalomyelitis.
abstract: Experimental allergic encephalomyelitis is a mouse model of human multiple sclerosis with similar pathology and pathogenesis. Th1 cells play an important role in the pathogenesis of experimental allergic encephalomyelitis. This study determined the potential effect of programmed cell death 1 ligand 1 in the pathogenesis of experimental allergic encephalomyelitis induced by injecting myelin oligodendrocyte glycoprotein, complete Freund's adjuvant and Bordetella pertussis toxin into C57BL/6J mice. Experimental allergic encephalomyelitis mice developed disease and showed inflammatory changes in the central nervous system by hematoxylin-eosin staining of spinal cord pathological sections, demyelination by Luxol fast-blue staining and clinical manifestations. The expression of programmed cell death 1 ligand 1 in mice was detected by immunohistochemistry, flow cytometry and western blot analysis. The expression of programmed cell death 1 ligand 1 in the spinal cord and splenocytes of mice was significantly increased compared with normal mice. Our findings suggest the involvement of programmed cell death 1 ligand 1 in the pathogenesis of experimental allergic encephalomyelitis and suggest this should be studied in multiple sclerosis.
authors.affiliation: Department of Neurology, Second People's Hospital of Lianyungang City, Lianyungang 222006, Jiangsu Province, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Neurology, Second People's Hospital of Lianyungang City, Lianyungang 222006, Jiangsu Province, China.
authors.forename: Jiandong
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Neurology, Second People's Hospital of Lianyungang City, Lianyungang 222006, Jiangsu Province, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Fu
authors.affiliation: Department of Neurology, Second People's Hospital of Lianyungang City, Lianyungang 222006, Jiangsu Province, China.
authors.forename: Jiechun
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Neurology, First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China ; Institute of Clinical Immunology, Soochow University, Suzhou 215006, Jiangsu Province, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Xue
authors.affiliation: Department of Neurology, First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China ; Institute of Clinical Immunology, Soochow University, Suzhou 215006, Jiangsu Province, China.
authors.forename: Wanli
authors.initials: W
authors.lastname: Dong
authors.affiliation: Institute of Clinical Immunology, Soochow University, Suzhou 215006, Jiangsu Province, China.
authors.forename: Yanzheng
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Department of Neurology, Third Hospital of Xingtai City, Xingtai 054000, Hebei Province, China.
authors.forename: Lingtao
authors.initials: L
authors.lastname: Tang
authors.affiliation: Department of Neurology, First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Xue
authors.affiliation: Department of Neurology, First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Fang
authors.affiliation: Red-Cross Blood Center of Suzhou City, Suzhou 215006, Jiangsu Province, China.
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Wang
authors.affiliation: Institute of Clinical Immunology, Soochow University, Suzhou 215006, Jiangsu Province, China.
authors.forename: Xueguang
authors.initials: X
authors.lastname: Zhang
keywords: B7-CD28 superfamily; animal models; autoimmune disease; costimulatory molecule; costimulatory signal; experimental allergic encephalomyelitis; grants-supported paper; multiple sclerosis; neural regeneration; neuroregeneration; programmed cell death 1 ligand 1