title: Effect of rhynchophylline on conditioned place preference on expression of NR2B in methamphetamine-dependent mice.
abstract: OBJECTIVE
  To study the effect of rhynchophylline on N-methyl d-aspartate receptor subtype 2B subunit in hippocampus of Methamphetamine-induced conditioned place preference (CPP) mice.
  
  
  METHODS
  Place preference mice models were established by methamphetamine; the expression of NR2B was observed by immunohistochemistry technique and Western blot.
  
  
  RESULTS
  Methamphetamine (4mg/kg)-induced place preference mice model was successfully established; ketamine (15mg/kg), rhynchophylline (40mg/kg) and rhynchophylline (80mg/kg) can eliminate place preference; Immunohistochemistry showed that the number of NR2B-positive neurons in hippocampus was increased in the methamphetamine model group, whereas less NR2B-positive neurons were found in the ketamine group, low and high dosage rhynchophylline group. Western blot showed that the expression of NR2B protein was significantly increased in the model group, whereas less expression was found in the ketamine group, low and high dosage rhynchophylline group.
  
  
  CONCLUSIONS
  NR2B plays an important role in the formation of methamphetamine-induced place preference in mice. Rhynchophylline reversed the expression of NR2B in the hippocampus demonstrates the potential effect of mediates methamphetamine induced rewarding effect.
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Junkui
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China; Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong.
authors.forename: Qiuxian
authors.initials: Q
authors.lastname: Peng
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Mingjin
authors.initials: M
authors.lastname: Jiang
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Chaohua
authors.initials: C
authors.lastname: Luo
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Youli
authors.initials: Y
authors.lastname: Guo
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Fang
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China. Electronic address: cherrymogz@gmail.com.
authors.forename: Zhixian
authors.initials: Z
authors.lastname: Mo
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D003216:Conditioning, Operant / Q000187:drug effects; D004334:Drug Administration Schedule; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D026121:Indole Alkaloids / Q000494:pharmacology*; D007649:Ketamine / Q000494:pharmacology; D008297:Male; D008694:Methamphetamine / Q000009:adverse effects*; D051379:Mice; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D000078183:Oxindoles; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D012201:Reward; D019966:Substance-Related Disorders / Q000188:drug therapy* / Q000209:etiology / Q000235:genetics / Q000503:physiopathology
chemical_list: D000470:Alkaloids; D018691:Excitatory Amino Acid Antagonists; D026121:Indole Alkaloids; C121001:NR2B NMDA receptor; D000078183:Oxindoles; D016194:Receptors, N-Methyl-D-Aspartate; D008694:Methamphetamine; C052714:rhyncophylline; D007649:Ketamine
keywords: Conditioned place preference; Hippocampal; Methamphetamine; NR2B; Rhynchophylline