title: Examining estrogen regulation of cancer stem cells through multicolor lineage tracing.
abstract: We have designed a dual-color fluorescent reporter that can track microRNA expression in vitro, which can be used for lineage tracing experiments. We have used this system to track miR-140 promoter activity in breast cancer cells and to follow the impact of estrogen signaling in cancer stem cell subpopulations.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Maryland Baltimore School of Medicine, Biomedical Research Facility, 337, Baltimore, MD, 21210, USA.
authors.forename: Yongshu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Gabriel
authors.initials: G
authors.lastname: Eades
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D005456:Fluorescent Dyes / Q000032:analysis / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000235:genetics*; D008856:Microscopy, Fluorescence / Q000379:methods; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D015398:Signal Transduction; D014162:Transfection / Q000379:methods
chemical_list: D004967:Estrogens; D005456:Fluorescent Dyes; D035683:MicroRNAs; C544302:Mirn140 microRNA, human