title: Antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells.
abstract: Antioxidants were implicated as potential reagents to enhance osteogenesis, and nano-fullerenes have been demonstrated to have a great antioxidative capacity by both in vitro and in vivo experiments. In this study, we assessed the impact of a polyhydroxylated fullerene, fullerol, on the osteogenic differentiation of human adipose-derived stem cells (ADSCs). Fullerol was not toxic against human ADSCs at concentrations up to 10 μM. At a concentration of 1 μM, fullerol reduced cellular reactive oxygen species after a 5-day incubation either in the presence or in the absence of osteogenic media. Pretreatment of fullerol for 7 days increased the osteogenic potential of human ADSCs. Furthermore, when incubated together with osteogenic medium, fullerol promoted osteogenic differentiation in a dose-dependent manner. Finally, fullerol proved to promote expression of FoxO1, a major functional isoform of forkhead box O transcription factors that defend against reactive oxygen species in bone. Although further clarification of related mechanisms is required, the findings may help further development of a novel approach for bone repair, using combined treatment of nano-fullerol with ADSCs.
authors.affiliation: Department of Orthopaedic Surgery, University of Virginia School of Medicine, Charlottesville, VA, USA.
authors.forename: Xinlin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Orthopaedic Surgery, University of Virginia School of Medicine, Charlottesville, VA, USA.
authors.forename: Ching-Ju
authors.initials: CJ
authors.lastname: Li
authors.affiliation: Department of Orthopaedic Surgery, University of Virginia School of Medicine, Charlottesville, VA, USA.
authors.forename: Yueping
authors.initials: Y
authors.lastname: Wan
authors.affiliation: Department of Orthopaedic Surgery, University of Virginia School of Medicine, Charlottesville, VA, USA.
authors.forename: Pinar
authors.initials: P
authors.lastname: Smith
authors.affiliation: Department of Orthopaedic Surgery, University of Virginia School of Medicine, Charlottesville, VA, USA.
authors.forename: Guowei
authors.initials: G
authors.lastname: Shang
authors.affiliation: Department of Orthopaedic Surgery, University of Virginia School of Medicine, Charlottesville, VA, USA.
authors.forename: Quanjun
authors.initials: Q
authors.lastname: Cui
mesh_terms: D017667:Adipocytes / Q000187:drug effects*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000378:metabolism; D037741:Fullerenes / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism
chemical_list: D000975:Antioxidants; C085126:FOXO1 protein, human; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; D037741:Fullerenes; D017382:Reactive Oxygen Species
keywords: bone repair; forkhead box protein O1; polyhydroxylated fullerene; reactive oxygen species