title: Cofactor profiling of the glucocorticoid receptor from a cellular environment.
abstract: The Microarray Assay for Realtime Coregulator-Nuclear receptor Interaction (MARCoNI) technology allows the identification of nuclear receptor-coregulator interactions via flow-through microarrays. As such, differences in the coregulator profile between distinct nuclear receptors or of a single receptor in agonist or antagonist mode can be investigated, even in a single run. In this chapter, the method how to perform these peptide microarrays with cell lysates containing the overexpressed glucocorticoid receptor is described, as well as the influence of assay parameters, variations to the protocol, and data analysis.
authors.affiliation: Cytokine Receptor Lab, VIB Department of Medical Protein Research, Department of Biochemistry, University of Ghent, Albert Baertsoenkaai 3, 9000, Ghent, Belgium.
authors.forename: Sofie J
authors.initials: SJ
authors.lastname: Desmet
authors.forename: Lien
authors.initials: L
authors.lastname: Dejager
authors.forename: Dorien
authors.initials: D
authors.lastname: Clarisse
authors.forename: Jonathan
authors.initials: J
authors.lastname: Thommis
authors.forename: Diana
authors.initials: D
authors.lastname: Melchers
authors.forename: Niek
authors.initials: N
authors.lastname: Bastiaensen
authors.forename: Rob
authors.initials: R
authors.lastname: Ruijtenbeek
authors.forename: Ilse M
authors.initials: IM
authors.lastname: Beck
authors.forename: Claude
authors.initials: C
authors.lastname: Libert
authors.forename: René
authors.initials: R
authors.lastname: Houtman
authors.forename: Onno C
authors.initials: OC
authors.lastname: Meijer
authors.forename: Karolien
authors.initials: K
authors.lastname: De Bosscher
mesh_terms: D000818:Animals; D057809:HEK293 Cells; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods*; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D012984:Software
chemical_list: D011965:Receptors, Glucocorticoid