title: RNA interference of the P450 CYP6CM1 gene has different efficacy in B and Q biotypes of Bemisia tabaci.
abstract: BACKGROUND
  Cytochrome P450 monooxygenases have been proven to be associated with high resistance in Bemisia tabaci B biotype (Middle East-Asia Minor 1 genetic group) and Q biotype (Mediterranean genetic group) to the neonicotinoid class of insecticides. In this study, the RNA interference (RNAi) effects on P450 CYP6CM1 gene expression, mortality and pesticide-detoxifying ability between B. tabaci B and Q biotypes were compared in an attempt to provide a basis for potential RNAi application in management of this pest.
  
  
  RESULTS
  Double-stranded RNAs (dsRNAs) of P450 CYP6CM1 genes corresponding to the B and Q biotypes were synthesised using specific primers and introduced into the insect body of B. tabaci adults through membrane feeding. The results showed that dsRNAs significantly silenced the target genes in B. tabaci with dsRNA concentration or treatment time, and silencing was more effective in B biotype than in Q biotype. Feeding dsRNAs led to high mortality in both biotypes, with higher mortality in B biotype (up to 85.88%) than in Q biotype (up to 56.40%). In addition, ability to detoxify imidacloprid and nicotine was inhibited in dsRNA-treated adults of both biotypes, more efficiently in B biotype than in Q biotype.
  
  
  CONCLUSIONS
  RNA interference of the P450 CYP6CM1 gene reduced gene expression, increased mortality, and inhibited the ability to detoxify a pesticide or a plant secondary metabolite in both biotypes of B. tabaci, with better efficacy in B biotype than in Q biotype.
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan, 450002, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan, 450002, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan, 450002, China.
authors.forename: Rune
authors.initials: R
authors.lastname: Bai
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan, 450002, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan, 450002, China.
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Tang
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan, 450002, China.
authors.forename: Shiheng
authors.initials: S
authors.lastname: An
authors.affiliation: College of Agriculture, Food and Natural Resources, University of Missouri, Columbia, MO, 65211, USA.
authors.forename: Qisheng
authors.initials: Q
authors.lastname: Song
authors.affiliation: College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan, 450002, China.
authors.forename: Fengming
authors.initials: F
authors.lastname: Yan
mesh_terms: D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D003368:Gossypium / Q000737:chemistry; D006430:Hemiptera / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D007093:Imidazoles / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000378:metabolism*; D008297:Male; D000073943:Neonicotinoids; D009538:Nicotine / Q000378:metabolism*; D009574:Nitro Compounds / Q000378:metabolism*; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism
chemical_list: D007093:Imidazoles; D019476:Insect Proteins; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D012330:RNA, Double-Stranded; C082359:imidacloprid; D009538:Nicotine; D003577:Cytochrome P-450 Enzyme System
keywords: B biotype; Bemisia tabaci; CYP6CM1 gene, RNAi; Q biotype; cytochrome P450