title: Purification, crystallization and preliminary X-ray crystallographic analysis of TssL from Vibrio cholerae.
abstract: The type VI secretion system (T6SS) is a macromolecular complex that is conserved in Gram-negative bacteria. The T6SS secretes effector proteins into recipient cells in a contact-dependent manner in order to accomplish cooperative and competitive interactions with the cells. Although the composition and mechanism of the T6SS have been intensively investigated across many Gram-negative bacteria, to date structural information on T6SS components from the important pathogen Vibrio cholerae has been rare. Here, the cloning, purification, crystallization and preliminary X-ray crystallographic analysis of the cytoplasmic domain of TssL, an inner membrane protein of the T6SS, from V. cholerae are reported. Diffraction data were collected to 1.5 Å resolution using synchrotron radiation. The crystal belonged to the hexagonal space group P61, with unit-cell parameters a = 78.4, b = 78.4, c = 49.5 Å. The successful structural characterization of TssL from V. cholerae will contribute to understanding the role of the membrane-associated subunits of the T6SS in more detail.
authors.affiliation: Beamline Science Division, Pohang Accelerator Laboratory, Jigok-ro 127, Pohang 790-784, Republic of Korea.
authors.forename: Jae-Hee
authors.initials: JH
authors.lastname: Jeong
authors.affiliation: Department of Biology, Teachers College, Kyungpook National University, Daehak-ro 80, Buk-ku, Daegu 702-701, Republic of Korea.
authors.forename: Jeong Ho
authors.initials: JH
authors.lastname: Chang
authors.affiliation: Beamline Science Division, Pohang Accelerator Laboratory, Jigok-ro 127, Pohang 790-784, Republic of Korea.
authors.forename: Yeon Gil
authors.initials: YG
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D001483:Base Sequence; D002850:Chromatography, Gel; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D017931:DNA Primers; D008958:Models, Molecular; D008969:Molecular Sequence Data; D014734:Vibrio cholerae / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers
keywords: T6SS; TssL; Vibrio cholerae