title: Nasal mucosal inhalation of amyloid-beta peptide 3-10 defective adenovirus attenuates cytotoxicity induced by beta-amyloid (1-42).
abstract: Three-month-old Alzheimer's disease model transgenic mice were immunized with Aβ1-42 Plp-Adenovirus [Ad]-X-CMV-(Aβ3-10)10-CpG [AdCpG-(Aβ3-10)10] or AdCpG virus fluid via nasal mucosal inhalation, respectively. ELISA analysis of serum showed Aβ42 antibody titers were significantly increased in mice immunized with Aβ1-42 and AdCpG-(Aβ3-10)10. Concanavalin A and AdCpG-(Aβ3-10)10 stimulation significantly increased the number of proliferating spleen cells cultured from AdCpG(Aβ3-10)10 and Aβ42 groups compared with the control group. In the AdCpG(Aβ3-10)10 group, levels of interleukin (IL)-4 and IL-10 were increased, while those of IL-2 and interferon-γ were decreased. In the Aβ42 group, levels of IL-4, IL-10, IL-2 and interferon-γ were all increased. Experimental findings indicate that AdCpG-(Aβ3-10)10 vaccine can produce strong T helper 2 (Th2) humoral immune responses in addition to the production of Aβ42 antibody. The cellular immunologic response was weak and avoided Aβ1-42-mediated cytotoxicity.
authors.affiliation: Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Tongzi
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Wanshu
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Sha
authors.affiliation: Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Xing
authors.affiliation: Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Guo
authors.affiliation: Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Cao
keywords: Alzheimer's disease; NSFC grant; amyloid-beta peptide vaccine; cytokines; humoral immunity; immunotherapy; inflammation; nerve regeneration; neural regeneration; neurodegenerative disease