title: An integrated multifunctional platform based on biotin-doped conducting polymer nanowires for cell capture, release, and electrochemical sensing.
abstract: Here, we propose an integrated multifunctional system constructed by conductive disulfide-biotin-doped polypyrrole nanowires (SS-biotin-Ppy NWs) for capture, release, and in situ quantification of circulating tumor cells (CTCs). A well-ordered three-dimensional nanowire structure equipped with a monoclonal antibody offers a significant impact on the cell-capture efficiency, as well as on electrical- or glutathione (GSH)-mediated release of the captured cells. In addition, the electrochemical identification/detection of the captured cancer cells can be directly conducted on the same Ppy NW platform by using horseradish peroxidase (HRP)-labeled and anti-EpCAM-conjugated nanoparticles (HRP/anti-EpCAM Ppy NPs), showing very high sensitivity and specificity. The signal amplification can be clearly attributed to the catalytic response resulting from enzymatic reduction of hydrogen peroxide on Ppy NWs, consequently generating a greatly increased amperometric response with a detection range of 10 to 1 × 10(4) cells and a detection limit of as low as 10 cells. Overall, the proposed Ppy NWs not only present a promising platform for effective cell capture and release but also permit cytosensing capability for on-site analysis.
authors.affiliation: New Experimental Therapeutic Branch, National Cancer Center, 111 Jungbalsan-ro, Ilsamdong-gu, Goyang, Gyeonggi-do 410-769, South Korea.
authors.forename: Woo Young
authors.initials: WY
authors.lastname: Hong
authors.affiliation: New Experimental Therapeutic Branch, National Cancer Center, 111 Jungbalsan-ro, Ilsamdong-gu, Goyang, Gyeonggi-do 410-769, South Korea.
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Jeon
authors.affiliation: New Experimental Therapeutic Branch, National Cancer Center, 111 Jungbalsan-ro, Ilsamdong-gu, Goyang, Gyeonggi-do 410-769, South Korea.
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Lee
authors.affiliation: New Experimental Therapeutic Branch, National Cancer Center, 111 Jungbalsan-ro, Ilsamdong-gu, Goyang, Gyeonggi-do 410-769, South Korea. Electronic address: yncho@ncc.re.kr.
authors.forename: Youngnam
authors.initials: Y
authors.lastname: Cho
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation; D001710:Biotin / Q000737:chemistry*; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000295:instrumentation*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004867:Equipment Design; D006801:Humans; D007150:Immunohistochemistry / Q000295:instrumentation; D057230:Limit of Detection; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000175:diagnosis; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D001710:Biotin
keywords: Cancer diagnosis; Circulating tumor cells; Conducting polymer; Electrical stimulation; Immunosensor