title: Reversible positioning of single molecules inside zero-mode waveguides.
abstract: We have developed a hybrid nanopore/zero-mode waveguide device for single-molecule fluorescence and DNA sequencing applications. The device is a freestanding solid-state membrane with sub-5 nm nanopores that reversibly delivers individual biomolecules to the base of 70 nm diameter waveguides for interrogation. Rapid and reversible molecular loading is achieved by controlling the voltage across the device. Using this device we demonstrate protein and DNA loading with efficiency that is orders of magnitude higher than diffusion-based molecular loading.
authors.affiliation: Department of Physics and ‡Department of Chemistry/Chemical Biology, Northeastern University , 110 Forsyth Street, Boston, Massachusetts 02115, United States.
authors.forename: Joseph
authors.initials: J
authors.lastname: Larkin
authors.forename: Mathieu
authors.initials: M
authors.lastname: Foquet
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Turner
authors.forename: Jonas
authors.initials: J
authors.lastname: Korlach
authors.forename: Meni
authors.initials: M
authors.lastname: Wanunu
mesh_terms: D004247:DNA / Q000032:analysis / Q000378:metabolism; D004058:Diffusion; D004867:Equipment Design; D005453:Fluorescence; D008958:Models, Molecular; D058608:Nanopores / Q000648:ultrastructure*; D036103:Nanotechnology / Q000295:instrumentation*; D061848:Optical Imaging / Q000295:instrumentation*; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000295:instrumentation*
chemical_list: D011506:Proteins; D004247:DNA
keywords: DNA sequencing; SMRT-sequencing; nanophotonics; single molecule; zeptoliter