title: In vitro attenuation of thermal-induced protein denaturation by aerial parts of Artemisia scoparia.
abstract: The goal of this study was to explore the aerial parts of Artemisia scoparia (crude extract, total flavonoid contents, and aqueous fraction) for protein denaturation potential. The crude extract provoked marked attenuation of thermal-induced denatured protein in a concentration-dependent manner with maximum inhibition of 54.05 μg/mL at 500 μg/mL and IC50 of 449.66 μg/mL. When total flavonoid contents were studied, it illustrated most dominant activity concentration dependently with maximum amelioration of 62.16 μg/mL at 500 μg/mL and IC50 of 378.35 μg/mL. The aqueous fraction also exhibited significant activity with maximum of 56.75% inhibition at 500 μg/mL and IC50 of 445.10 μg/mL. It can be concluded on the basis of the results that the crude extract, flavonoid contents, and aqueous fraction of the plant possessed significant inhibition on thermal-induced denatured protein.
authors.affiliation: Department of Chemistry, Kohat University of Science and Technology, Kohat, Pakistan.
authors.forename: Murad Ali
authors.initials: MA
authors.lastname: Khan
authors.affiliation: Department of Pharmacy, Abdul Wali Khan University Mardan 23200, Pakistan hkdr2006@gmail.com.
authors.forename: Haroon
authors.initials: H
authors.lastname: Khan
authors.affiliation: Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan.
authors.forename: Shafiq Ahmad
authors.initials: SA
authors.lastname: Tariq
authors.affiliation: Department of Pharmacy, University of Peshawar, 25120, Pakistan.
authors.forename: Samreen
authors.initials: S
authors.lastname: Pervez
mesh_terms: D000418:Albumins / Q000737:chemistry; D000704:Analysis of Variance; D019306:Artemisia / Q000737:chemistry*; D001241:Aspirin / Q000494:pharmacology; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D011489:Protein Denaturation / Q000187:drug effects*
chemical_list: D000418:Albumins; D005419:Flavonoids; D010936:Plant Extracts; D001241:Aspirin
keywords: Artemisia scoparia; extract and aqueous fraction; flavonoid contents; protein denaturation potential