title: Identification of molecular markers associated with alteration of receptor-binding specificity in a novel genotype of highly pathogenic avian influenza A(H5N1) viruses detected in Cambodia in 2013.
abstract: Human infections with influenza A(H5N1) virus in Cambodia increased sharply during 2013. Molecular characterization of viruses detected in clinical specimens from human cases revealed the presence of mutations associated with the alteration of receptor-binding specificity (K189R, Q222L) and respiratory droplet transmission in ferrets (N220K with Q222L). Discovery of quasispecies at position 222 (Q/L), in addition to the absence of the mutations in poultry/environmental samples, suggested that the mutations occurred during human infection and did not transmit further.
authors.affiliation: Virology Unit/National Influenza Centre, Institut Pasteur in Cambodia, Phnom Penh, Cambodia.
authors.forename: Sareth
authors.initials: S
authors.lastname: Rith
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: C Todd
authors.initials: CT
authors.lastname: Davis
authors.affiliation: Virology Unit/National Influenza Centre, Institut Pasteur in Cambodia, Phnom Penh, Cambodia.
authors.forename: Veasna
authors.initials: V
authors.lastname: Duong
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA Centers for Disease Control and Prevention, Phnom Penh, Cambodia.
authors.forename: Borann
authors.initials: B
authors.lastname: Sar
authors.affiliation: Virology Unit/National Influenza Centre, Institut Pasteur in Cambodia, Phnom Penh, Cambodia.
authors.forename: Srey Viseth
authors.initials: SV
authors.lastname: Horm
authors.affiliation: National Institute of Public Health, Phnom Penh, Cambodia.
authors.forename: Savuth
authors.initials: S
authors.lastname: Chin
authors.affiliation: Communicable Disease Department, Ministry of Health, Phnom Penh, Cambodia.
authors.forename: Sovann
authors.initials: S
authors.lastname: Ly
authors.affiliation: Kantha Bopha Hospital, Phnom Penh, Cambodia.
authors.forename: Denis
authors.initials: D
authors.lastname: Laurent
authors.affiliation: Kantha Bopha Hospital, Phnom Penh, Cambodia.
authors.forename: Beat
authors.initials: B
authors.lastname: Richner
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA Epidemic Intelligence Service, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Ikwo
authors.initials: I
authors.lastname: Oboho
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Yunho
authors.initials: Y
authors.lastname: Jang
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: William
authors.initials: W
authors.lastname: Davis
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Sharmi
authors.initials: S
authors.lastname: Thor
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Amanda
authors.initials: A
authors.lastname: Balish
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: A Danielle
authors.initials: AD
authors.lastname: Iuliano
authors.affiliation: National Veterinary Research Institute, Ministry of Agriculture, Forestry and Fisheries, Phnom Penh, Cambodia.
authors.forename: San
authors.initials: S
authors.lastname: Sorn
authors.affiliation: National Veterinary Research Institute, Ministry of Agriculture, Forestry and Fisheries, Phnom Penh, Cambodia.
authors.forename: Davun
authors.initials: D
authors.lastname: Holl
authors.affiliation: Communicable Disease Department, Ministry of Health, Phnom Penh, Cambodia.
authors.forename: Touch
authors.initials: T
authors.lastname: Sok
authors.affiliation: Communicable Disease Department, Ministry of Health, Phnom Penh, Cambodia.
authors.forename: Heng
authors.initials: H
authors.lastname: Seng
authors.affiliation: Virology Unit/National Influenza Centre, Institut Pasteur in Cambodia, Phnom Penh, Cambodia.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Tarantola
authors.affiliation: World Health Organization, Phnom Penh, Cambodia.
authors.forename: Reiko
authors.initials: R
authors.lastname: Tsuyuoka
authors.affiliation: World Health Organization, Phnom Penh, Cambodia.
authors.forename: Amy
authors.initials: A
authors.lastname: Parry
authors.affiliation: World Health Organization, Phnom Penh, Cambodia.
authors.forename: Nora
authors.initials: N
authors.lastname: Chea
authors.affiliation: Food and Agriculture Organization, Phnom Penh, Cambodia.
authors.forename: Lotfi
authors.initials: L
authors.lastname: Allal
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA Centers for Disease Control and Prevention, Phnom Penh, Cambodia.
authors.forename: Paul
authors.initials: P
authors.lastname: Kitsutani
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA Centers for Disease Control and Prevention, Phnom Penh, Cambodia.
authors.forename: Dora
authors.initials: D
authors.lastname: Warren
authors.affiliation: U.S. Naval Medical Research Unit No. 2, Phnom Penh, Cambodia.
authors.forename: Michael
authors.initials: M
authors.lastname: Prouty
authors.affiliation: Virology Unit/National Influenza Centre, Institut Pasteur in Cambodia, Phnom Penh, Cambodia.
authors.forename: Paul
authors.initials: P
authors.lastname: Horwood
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Marc-Alain
authors.initials: MA
authors.lastname: Widdowson
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Lindstrom
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Julie
authors.initials: J
authors.lastname: Villanueva
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Ruben
authors.initials: R
authors.lastname: Donis
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Nancy
authors.initials: N
authors.lastname: Cox
authors.affiliation: Virology Unit/National Influenza Centre, Institut Pasteur in Cambodia, Phnom Penh, Cambodia buchyphilippe@hotmail.com.
authors.forename: Philippe
authors.initials: P
authors.lastname: Buchy
mesh_terms: D000293:Adolescent; D000328:Adult; D019943:Amino Acid Substitution; D002160:Cambodia; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D005260:Female; D005819:Genetic Markers; D005838:Genotype; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D006801:Humans; D007223:Infant; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology*; D007251:Influenza, Human / Q000821:virology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D053585:Virus Attachment
chemical_list: D005819:Genetic Markers; D019267:Hemagglutinin Glycoproteins, Influenza Virus