title: Effects of ozonated olive oil on acute radiation proctitis in rats.
abstract: BACKGROUND
  Acute radiation proctitis is a common complication of pelvic radiation and management of acute radiation proctitis is under evaluation. The beneficial effects of ozonated olive oil (OzOO) have already been shown in the treatment of chronic wounds. Thus, this study was designed to evaluate the therapeutic effects of topical OzOO on acute radiation proctitis.
  
  
  OBJECTIVE
  To evaluate the therapeutic effects of topical OzOO on acute radiation proctitis.
  
  
  METHODS
  Animal experimentation.
  
  
  METHODS
  RATS WERE DIVIDED INTO THREE GROUPS: control; irradiation+saline (1 mL); and irradiation +OzOO (1 mL). A single fraction of 17.5 Gy was delivered to each rat. The OzOO was administered rectally each day after irradiation. Each rat was observed daily for signs of proctitis. Irradiated rats were euthanised on days 5 and 10. The mucosal changes were evaluated macroscopically and pathologically.
  
  
  RESULTS
  According to the clinical findings, five rats in the irradiation+saline group showed Grade 4 symptoms on the 10(th) day. Macroscopic finding scores on the 10(th) day in the irradiation+saline and irradiation+OzOO groups were statistically significantly different. On pathological examination, radiation-induced mucosal damage was the most prominent 10 days after irradiation in saline-treated rats. On the 10(th) day, the irradiation+OzOO group showed mild inflammation and slight crypt change, which corresponded to Grade 1 pathological findings.
  
  
  CONCLUSIONS
  OzOO attenuates macroscopic and pathological findings of acute radiation proctitis in rats.
authors.affiliation: Department of General Surgery, Bülent Ecevit University Faculty of Medicine, Zonguldak, Turkey.
authors.forename: Fatma Ayça
authors.initials: FA
authors.lastname: Gültekin
authors.affiliation: Department of Radiation Oncology, Bülent Ecevit University Faculty of Medicine, Zonguldak, Turkey.
authors.forename: Bekir Hakan
authors.initials: BH
authors.lastname: Bakkal
authors.affiliation: Department of General Surgery, Bülent Ecevit University Faculty of Medicine, Zonguldak, Turkey.
authors.forename: Demet
authors.initials: D
authors.lastname: Sümer
authors.affiliation: Department of Biostatistics, Bülent Ecevit University Faculty of Medicine, Zonguldak, Turkey.
authors.forename: Füruzan
authors.initials: F
authors.lastname: Köktürk
authors.affiliation: Department of Pathology, Bülent Ecevit University Faculty of Medicine, Zonguldak, Turkey.
authors.forename: Sibel
authors.initials: S
authors.lastname: Bektaş
keywords: Ozonated olive oil; radiation proctitis