title: Effect of oral administration of Lactobacillus plantarum HY7714 on epidermal hydration in ultraviolet B-irradiated hairless mice.
abstract: In this study, we evaluated the effect of Lactobacillus plantarum HY7714 on skin hydration in human dermal fibroblasts and in hairless mice. In Hs68 cells, L. plantarum HY7714 not only increased the serine palmitoyltransferase (SPT) mRNA level, but also decreased the ceramidase mRNA level. In order to confirm the hydrating effects of L. plantarum HY7714 in vivo, we orally administered vehicle or L. plantarum HY7714 at a dose of 1 × 10(9) CFU/day to hairless mice for 8 weeks. In hairless mice, L. plantarum HY7714 decreased UVB-induced epidermal thickness. In addition, we found that L. plantarum HY7714 administration suppressed the increase in transepidermal water loss and decrease in skin hydration, which reflects barrier function fluctuations following UV irradiation. In particular, L. plantarum HY7714 administration increased the ceramide level compared with that in the UVB group. In the experiment on SPT and ceramidase mRNA expressions, L. plantarum HY7714 administration improved the reduction in SPT mRNA levels and suppressed the increase in ceramidase mRNA levels caused by UVB in the hairless mice skins. Collectively, these results suggest that L. plantarum HY7714 can be a potential candidate for preserving skin hydration levels against UV irradiation.
authors.affiliation: Korea Yakult Co., Ltd., Yongin 446-901, Republic of Korea.
authors.forename: Jehyeon
authors.initials: J
authors.lastname: Ra
authors.forename: Dong Eun
authors.initials: DE
authors.lastname: Lee
authors.forename: Sung Hwan
authors.initials: SH
authors.lastname: Kim
authors.forename: Ji-Woong
authors.initials: JW
authors.lastname: Jeong
authors.forename: Hyung Keun
authors.initials: HK
authors.lastname: Ku
authors.forename: Tae-Youl
authors.initials: TY
authors.lastname: Kim
authors.forename: Il-Dong
authors.initials: ID
authors.lastname: Choi
authors.forename: Woonhee
authors.initials: W
authors.lastname: Jeung
authors.forename: Jae-Hun
authors.initials: JH
authors.lastname: Sim
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Ahn
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002460:Cell Line; D055572:Ceramidases / Q000096:biosynthesis; D005347:Fibroblasts / Q000502:physiology; D020869:Gene Expression Profiling; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000254:growth & development*; D008812:Mice, Hairless; D019936:Probiotics / Q000008:administration & dosage*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051102:Serine C-Palmitoyltransferase / Q000096:biosynthesis; D012867:Skin / Q000201:enzymology / Q000528:radiation effects*; D012879:Skin Physiological Phenomena / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D012333:RNA, Messenger; D051102:Serine C-Palmitoyltransferase; D055572:Ceramidases