title: Development of SSR markers for Encholirium horridum (Bromeliaceae) and transferability to other Pitcairnioideae.
abstract: OBJECTIVE
  We developed a set of primers for Encholirium horridum, a species closely associated with inselbergs of the Atlantic Forest, to assess genetic diversity, genetic structure, and gene flow between populations of this species. •
  
  
  RESULTS
  From an enriched genomic library, 10 primer pairs for polymorphic microsatellite regions were developed. The average number of alleles ranged from eight to 20, and the observed and expected heterozygosities ranged from 0.000 to 1.000, and from 0.000 to 0.929, respectively, across the populations. •
  
  
  CONCLUSIONS
  These markers will be useful in evaluating genetic diversity, spatial genetic structure, analysis of gene flow by paternity, and characterization of mating system of E. horridum.
authors.affiliation: Jardim Botânico do Rio de Janeiro, 22460-030 Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Karina Vanessa
authors.initials: KV
authors.lastname: Hmeljevski
authors.affiliation: ESALQ/Universidade de São Paulo, 13418-900 Piracicaba, São Paulo, Brazil.
authors.forename: Maísa B
authors.initials: MB
authors.lastname: Ciampi
authors.affiliation: Universidade Federal Rural do Semiárido, 59625-900 Mossoró, Rio Grande do Norte, Brazil.
authors.forename: Cristina
authors.initials: C
authors.lastname: Baldauf
authors.affiliation: NPFT/Universidade Federal de Santa Catarina, P.O. Box 476, 88040-900 Florianópolis, Santa Catarina, Brazil.
authors.forename: Maurício
authors.initials: M
authors.lastname: Sedrez Dos Reis
authors.affiliation: Jardim Botânico do Rio de Janeiro, 22460-030 Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Rafaela Campostrini
authors.initials: RC
authors.lastname: Forzza
keywords: Atlantic Forest; Bromeliaceae; Encholirium horridum; cross-amplification; inselberg; microsatellites