title: Inhalation of Shin-I essential oil enhances lactate clearance in treadmill exercise.
abstract: OBJECTIVE
  To evaluate the effect of Shin-I essential oil inhalation on blood lactate changes in rats subjected to treadmill exercise.
  
  
  METHODS
  : Adult male Sprague Dawley rats (n=12) were randomly divided into the control or the Shin-I group. Rats were subjected to a treadmill exercise program (15 m/min for 30 min). After exercise, rats were exposed to 200 µL of water or Shin-I essential oil, respectively, using a nebulizer for 180 min during the recovery period. Blood samples were collected every 15 min. Blood glucose and lactate concentrations were determined in a CMA 600 analyzer.
  
  
  RESULTS
  : The basal glucose and lactate levels were no significantly different between two groups. After exercise, glucose levels were slightly increased to about 110%-120% of the basal level in both groups. Lactate levels of both groups reached to 110%-140% of basal levels during exercise. In the recovery period, lactate levels further increased to 180% of the basal level and were maintained at a plateau in the control group. However, lactate levels gradually decreased to 60%-65% of the basal level in the Shin-I group. Lactate clearance was significantly enhanced after Shin-I essential oil inhalation.
  
  
  CONCLUSIONS
  : Our results provide evidence that Shin-I essential oil inhalation may accelerate recovery after exercise in rats.
authors.affiliation: Stem Cell Center, Department of Medical Research, Taichung Veterans General Hospital, 1650, Sect. 4, Taiwan Boulevard, Taichung, 40705, Taiwan ; Department of Food and Nutrition, Providence University, 200, Sec. 7, Taiwan Boulevard, Shalu Dist., Taichung, 43301, Taiwan.
authors.forename: Hsuan-Ying
authors.initials: HY
authors.lastname: Chen
authors.affiliation: Department of Food and Nutrition, Providence University, 200, Sec. 7, Taiwan Boulevard, Shalu Dist., Taichung, 43301, Taiwan.
authors.forename: Ming-Fu
authors.initials: MF
authors.lastname: Wang
authors.affiliation: Yangsen Biotechnology Co., Ltd. 3F, 5, Sec.1, Bei-tou.Rd., Bei-tou, Taipei, 11245, Taiwan.
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Lin
authors.affiliation: Institute of Biochemistry and Molecular Biology, National Yang-Ming University, 155, Sec.2, Li-nong Street, Taipei, 11221, Taiwan.
authors.forename: Ying-Chieh
authors.initials: YC
authors.lastname: Tsai
authors.affiliation: Stem Cell Center, Department of Medical Research, Taichung Veterans General Hospital, 1650, Sect. 4, Taiwan Boulevard, Taichung, 40705, Taiwan.
authors.forename: Fu-Chou
authors.initials: FC
authors.lastname: Cheng
keywords: Blood; Lactate; Shin-I essential oil; Treadmill exercise