title: Characterisation of secretory calcium-binding phosphoprotein-proline-glutamine-rich 1: a novel basal lamina component expressed at cell-tooth interfaces.
abstract: Functional genomic screening of the rat enamel organ (EO) has led to the identification of a number of secreted proteins expressed during the maturation stage of amelogenesis, including amelotin (AMTN) and odontogenic ameloblast-associated (ODAM). In this study, we characterise the gene, protein and pattern of expression of a related protein called secretory calcium-binding phosphoprotein-proline-glutamine-rich 1 (SCPPPQ1). The Scpppq1 gene resides within the secretory calcium-binding phosphoprotein (Scpp) cluster. SCPPPQ1 is a highly conserved, 75-residue, secreted protein rich in proline, leucine, glutamine and phenylalanine. In silico data mining has revealed no correlation to any known sequences. Northern blotting of various rat tissues suggests that the expression of Scpppq1 is restricted to tooth and associated tissues. Immunohistochemical analyses show that the protein is expressed during the late maturation stage of amelogenesis and in the junctional epithelium where it localises to an atypical basal lamina at the cell-tooth interface. This discrete localisation suggests that SCPPPQ1, together with AMTN and ODAM, participates in structuring the basal lamina and in mediating attachment of epithelia cells to mineralised tooth surfaces.
authors.affiliation: Shriners Hospital for Children, Montréal, Québec, Canada.
authors.forename: Pierre
authors.initials: P
authors.lastname: Moffatt
authors.forename: Rima M
authors.initials: RM
authors.lastname: Wazen
authors.forename: Juliana
authors.initials: J
authors.lastname: Dos Santos Neves
authors.forename: Antonio
authors.initials: A
authors.lastname: Nanci
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000378:metabolism; D001483:Base Sequence; D001485:Basement Membrane / Q000378:metabolism*; D015152:Blotting, Northern; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D057809:HEK293 Cells; D006639:Histidine; D006801:Humans; D051379:Mice; D008963:Molar / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D009842:Oligopeptides; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014070:Tooth / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D014162:Transfection
chemical_list: D000906:Antibodies; D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; C471213:His-His-His-His-His-His; C000599303:LOC100359907; D009842:Oligopeptides; D010750:Phosphoproteins; D011994:Recombinant Proteins; C000599302:SCPPPQ1 protein, mouse; D006639:Histidine