title: Low pH capillary electrophoresis application to improve capillary electrophoresis-systematic evolution of ligands by exponential enrichment.
abstract: In this work, a novel low pH CE-SELEX (LpH-CE-SELEX) as a CE-SELEX variant is proposed. Transferring (Trf), bovine serum albumin (BSA) and cytochrome c (Cyt c) as model protein are incubated with a FAM labeled ssDNA library, respectively. Incubation mixture is separated in low pH CE (pH 2.6), where positively charged protein, protein-ssDNA complex and negatively charged ssDNA library migrate oppositely without EOF driven. Analysis of protein-ssDNA complex under positive voltage and unbound ssDNA library under negative voltage by CE-UV are applied for interactive evaluation. By increasing injection time, larger amount protein-ssDNA complex can be collected conveniently at the cathode end whereas ssDNA migrates to anode. Finally, stability of protein-ssDNA complex in low pH CE separation is discussed.
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Beijing Centre for Physical and Chemical Analysis, Beijing 100089, China.
authors.forename: Xinying
authors.initials: X
authors.lastname: Zhao
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
authors.forename: Hongyang
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, China. Electronic address: qufengqu@bit.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Qu
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000378:metabolism; D002417:Cattle; D045304:Cytochromes c / Q000302:isolation & purification; D004247:DNA / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D052156:SELEX Aptamer Technique / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification; D014168:Transferrin / Q000302:isolation & purification
chemical_list: D052157:Aptamers, Nucleotide; D008024:Ligands; D014168:Transferrin; D012710:Serum Albumin, Bovine; D045304:Cytochromes c; D004247:DNA
keywords: Aptamers selection; Low pH CE-SELEX; Low pH capillary electrophoresis; Protein