title: Correlation between histopathological and FT-Raman spectroscopy analysis of the liver of Swiss mice infected with Paracoccidioides brasiliensis.
abstract: Paracoccidioidomycosis is the most important systemic mycosis in Latin America. The main entrance of the fungus is the airway. It primarily occurs in the lung, but in its disseminated form may affect any organ. The liver is one of the organs afflicted by this disease and its homeostasis may be impaired. The aim of the present study was to evaluate the evolution of paracoccidioidomycosis in the liver of Swiss mice and correlate morphological factors with the expression of gp43 and with physicochemical analysis via FT-Raman of the infected organ. According to colony forming unit (CFU) and granuloma counting, the first and second weeks were the periods when infection was most severe. Tissue response was characterized by the development of organized granulomas and widespread infection, with yeasts located within the macrophages and isolated hepatocytes. The gp43 molecule was distributed throughout the hepatic parenchyma, and immunostaining was constant in all observed periods. The main physicochemical changes of the infected liver were observed in the spectral ranges between 1700-1530 cm(-1) and 1370-1290 cm(-1), a peak shifting center attributed to phenylalanine and area variation of -CH2 and -CH3 compounds associated to collagen, respectively. Over time, there was a direct proportional relationship between the number of CFUs, the number of granulomas and the physicochemical changes in the liver of mice infected with Paracoccidioides brasiliensis. The expression of gp43 was similar in all observed periods.
authors.affiliation: Department of Morphological Sciences, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Elaine Sciuniti Benites
authors.initials: ES
authors.lastname: Mansano
authors.affiliation: Department of Physics, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Gutierrez Rodrigues
authors.initials: GR
authors.lastname: de Morais
authors.affiliation: Department of Morphological Sciences, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Edilaine Martins
authors.initials: EM
authors.lastname: Moratto
authors.affiliation: Department of Physics, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Francielle
authors.initials: F
authors.lastname: Sato
authors.affiliation: Department of Physics, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Antonio
authors.initials: A
authors.lastname: Medina Neto
authors.affiliation: Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Terezinha Ines Estivalet
authors.initials: TI
authors.lastname: Svidzinski
authors.affiliation: Department of Physics, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Mauro Luciano
authors.initials: ML
authors.lastname: Baesso
authors.affiliation: Department of Morphological Sciences, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Luzmarina
authors.initials: L
authors.lastname: Hernandes
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial; D005583:Fourier Analysis; D006099:Granuloma / Q000473:pathology; D008099:Liver / Q000382:microbiology* / Q000473:pathology*; D008297:Male; D051379:Mice; D010228:Paracoccidioides / Q000254:growth & development / Q000502:physiology*; D010229:Paracoccidioidomycosis / Q000382:microbiology* / Q000473:pathology*; D013059:Spectrum Analysis, Raman; D013997:Time Factors