title: Effect of probiotic L. plantarum IS-10506 and zinc supplementation on humoral immune response and zinc status of Indonesian pre-school children.
abstract: A 90-day randomized, double-blind, placebo-controlled, pre-post trial was conducted in four groups of Indonesian children aged 12-24 months: placebo, probiotic, zinc, and a combination of probiotic and zinc (n=12 per group). Microencapsulated Lactobacillus plantarum IS-10506 of dadih origin was supplemented at a dose of 10(10)CFU/day as a probiotic. Zinc was supplemented as 20mg zinc sulfate monohydrate (8mg zinc elemental). Blood and stool samples were collected at baseline and at the end of the study period. Fecal sIgA was assessed by ELISA and serum zinc concentrations by ICP-MS. Fecal sIgA increased significantly in the probiotic group (30.33±3.32μg/g; p<0.01) and in the combination probiotic and zinc group (27.55±2.28μg/g; p<0.027), as compared with the placebo group (13.58±2.26μg/g). Changes in serum zinc concentrations in the combination probiotic and zinc group showed the highest elevation at the end of the study period. A combination of probiotic L. plantarum IS-10506 at a dose of 10(10)CFU/day and 8mg of elemental zinc supplementation showed a potential ability to improve the zinc status of pre-school children. Taken together, supplementation with the probiotic L. plantarum IS-10506 and zinc for 90 days resulted in a significantly increased humoral immune response, as well as improved zinc status, in young children.
authors.affiliation: Food Technology Department, Faculty of Engineering, BINUS University, Alam Sutra Campus, Tangerang, Indonesia; SEAMEO RECFON University of Indonesia, Salemba Raya 6, Jakarta, Indonesia. Electronic address: isurono@binus.edu.
authors.forename: Ingrid S
authors.initials: IS
authors.lastname: Surono
authors.affiliation: Faculty of Medicine, Indonesian Christian University, Jl. Mayjen Sutoyo Cawang, Jakarta 13630, Indonesia.
authors.forename: Pratiwi D
authors.initials: PD
authors.lastname: Martono
authors.affiliation: Department of Public Health, Gunma University, Graduate School of Medicine, 39-22 Showa-machi 3-chome, Maebashi, Gunma 371-8511, Japan.
authors.forename: Satomi
authors.initials: S
authors.lastname: Kameo
authors.affiliation: Department of Public Health, Gunma University, Graduate School of Medicine, 39-22 Showa-machi 3-chome, Maebashi, Gunma 371-8511, Japan.
authors.forename: Eka W
authors.initials: EW
authors.lastname: Suradji
authors.affiliation: Department of Public Health, Gunma University, Graduate School of Medicine, 39-22 Showa-machi 3-chome, Maebashi, Gunma 371-8511, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Koyama
mesh_terms: D002675:Child, Preschool; D004311:Double-Blind Method; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D056724:Immunity, Humoral / Q000187:drug effects*; D007071:Immunoglobulin A, Secretory / Q000378:metabolism; D007223:Infant; D048191:Lactiplantibacillus plantarum / Q000502:physiology*; D008297:Male; D019936:Probiotics / Q000494:pharmacology*; D015032:Zinc / Q000097:blood*
chemical_list: D007071:Immunoglobulin A, Secretory; D015032:Zinc
keywords: Fecal sIgA; Probiotic L. plantarum IS-10506; Serum zinc; Zinc