title: Validation of analytical breast cancer microarray analysis in medical laboratory.
abstract: A previously reported microarray data analysis by RISS algorithm on breast cancer showed over-expression of the growth factor receptor (Grb7) and it also highlighted Tweety (TTYH1) gene to be under expressed in breast cancer for the first time. Our aim was to validate the results obtained from the microarray analysis with respect to these genes. Also, the relationship between their expression and the different prognostic indicators was addressed. RNA was extracted from the breast tissue of 30 patients with primary malignant breast cancer. Control samples from the same patients were harvested at a distance of ≥5 cm from the tumour. Semi-quantitative RT-PCR analysis was done on all samples. There was a significant difference between the malignant and control tissues as regards Grb7 expression. It was significantly related to the presence of lymph node metastasis, stage and histological grade of the malignant tumours. There was a significant inverse relation between expression of Grb7 and expression of both oestrogen and progesterone receptors. Grb7 was found to be significantly related to the biological classification of breast cancer. TTYH1 was not expressed in either the malignant or the control samples. The RISS by our group algorithm developed was laboratory validated for Grb7, but not for TTYH1. The newly developed software tool needs to be improved.
authors.affiliation: Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo, 11381, Egypt.
authors.forename: Amal Said
authors.initials: AS
authors.lastname: Darweesh
authors.forename: Manal Louis
authors.initials: ML
authors.lastname: Louka
authors.forename: Maha
authors.initials: M
authors.lastname: Hana
authors.forename: Shaymaa
authors.initials: S
authors.lastname: Rashad
authors.forename: Mohamed
authors.initials: M
authors.lastname: El-Shinawi
authors.forename: Ahmed
authors.initials: A
authors.lastname: Sharaf-Eldin
authors.forename: Samar Kamal
authors.initials: SK
authors.lastname: Kassim
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D004534:Egypt; D005260:Female; D051382:GRB7 Adaptor Protein / Q000235:genetics*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D046228:Microarray Analysis / Q000379:methods*; D008875:Middle Aged; D011379:Prognosis; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors
chemical_list: C495955:GRB7 protein, human; D008565:Membrane Proteins; C417913:TTYH1 protein, human; D051382:GRB7 Adaptor Protein