title: In situ label-free visualization of orally dosed strictinin within mouse kidney by MALDI-MS imaging.
abstract: Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is a powerful technique for visualizing the distribution of a wide range of biomolecules within tissue sections. However, methodology for visualizing a bioactive ellagitannin has not yet been established. This paper presents a novel in situ label-free MALDI-MSI technique for visualizing the distribution of strictinin, a bioactive ellagitannin found in green tea, within mammalian kidney after oral dosing. Among nine representative matrix candidates, 1,5-diaminonaphthalene (1,5-DAN), harmane, and ferulic acid showed higher sensitivity to strictinin spotted onto a MALDI sample plate. Of these, 1,5-DAN enables visualization of a two-dimensional image of strictinin directly spotted on mouse kidney sections with the highest sensitivity. Furthermore, 1,5-DAN-based MALDI-MSI could detect the unique distribution of orally dosed strictinin within kidney sections. This in situ label-free imaging technique will contribute to the localization analysis of strictinin and its biological mechanisms.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University , 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Yoon Hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Fujimura
authors.forename: Masako
authors.initials: M
authors.lastname: Sasaki
authors.forename: Xue
authors.initials: X
authors.lastname: Yang
authors.forename: Daichi
authors.initials: D
authors.lastname: Yukihira
authors.forename: Daisuke
authors.initials: D
authors.lastname: Miura
authors.forename: Yumi
authors.initials: Y
authors.lastname: Unno
authors.forename: Koretsugu
authors.initials: K
authors.lastname: Ogata
authors.forename: Hiroki
authors.initials: H
authors.lastname: Nakajima
authors.forename: Shuya
authors.initials: S
authors.lastname: Yamashita
authors.forename: Kanami
authors.initials: K
authors.lastname: Nakahara
authors.forename: Motoki
authors.initials: M
authors.lastname: Murata
authors.forename: I-Chian
authors.initials: IC
authors.lastname: Lin
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Wariishi
authors.forename: Koji
authors.initials: K
authors.lastname: Yamada
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Tachibana
mesh_terms: D000818:Animals; D028241:Camellia sinensis / Q000378:metabolism*; D007668:Kidney / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D010636:Phenols / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism*; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D010636:Phenols; D010936:Plant Extracts; C496786:strictinin
keywords: MALDI-MS imaging; distribution; ellagitannin; green tea; strictinin