title: Effects of disinfectant agents on microleakage in primary tooth-colored restorations: an in vitro study.
abstract: OBJECTIVE
  To determine the effect of disinfectant agents on microleakage in primary teeth restored with composite resin (CR) and glass ionomer (GI).
  
  
  METHODS
  Class V cavities were prepared in 140 sound canine teeth randomly divided into two main groups (A and B) and seven subgroups (n=20). In group A, 60 cavities were restored with CR after pretreatment with 1) phosphoric acid etching + Adper Single-Bond 2; 2) acid etching + chlorhexidine digluconate + Adper SingleBond 2; and 3) Clearfil Protect Bond. In group B, 80 cavities were restored with GI after pretreatment with 4) conditioner; 5) conditioner + chlorhexidine digluconate; 6) conditioner + Clearfil Protect Bond; and 7) Clearfil Protect Bond. After thermocycling, the samples were placed in 0.5 percent fuchsin dye, sectioned, and evaluated under a digital microscope. Statistical analysis was done using Kruskal-Wallis and Mann-Whitney tests.
  
  
  RESULTS
  There were statistically significant differences between subgroups (P<.05). Subgroup 1 showed the least microleakage in Group A. Subgroups 6 and 7 had the least microleakage, followed by subgroups 4 and 5 in Group B.
  
  
  CONCLUSIONS
  Applying chlorhexidine digluconate and Clearfil Protect Bond increased microleakage in CR restorations. However, Clearfil Protect Bond did not adversely affect marginal sealing in GI restorations, which makes it an alternative pretreatment method for these restorations.
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mahtab
authors.initials: M
authors.lastname: Memarpour
authors.affiliation: Department of Operative Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran. shafief@sums.ac.ir.
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Shafiei
authors.affiliation: Department of Operative Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Marzieh
authors.initials: M
authors.lastname: Moradian
mesh_terms: D000134:Acid Etching, Dental; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002710:Chlorhexidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D003481:Cuspid; D003731:Dental Caries / Q000628:therapy*; D003738:Dental Cements / Q000494:pharmacology*; D003763:Dental Leakage / Q000517:prevention & control*; D018772:Dental Marginal Adaptation; D003793:Dental Restoration, Permanent; D017220:Dentin-Bonding Agents / Q000494:pharmacology*; D004202:Disinfectants / Q000494:pharmacology*; D005899:Glass Ionomer Cements / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D010756:Phosphoric Acids / Q000494:pharmacology; D011897:Random Allocation; D014094:Tooth, Deciduous
chemical_list: C554641:Adper single bond 2; D000891:Anti-Infective Agents, Local; C503075:Clearfil Protect Bond; D003738:Dental Cements; D017220:Dentin-Bonding Agents; D004202:Disinfectants; D005899:Glass Ionomer Cements; D010756:Phosphoric Acids; C030242:phosphoric acid; C010882:chlorhexidine gluconate; D002710:Chlorhexidine