title: Identification of a novel cytochrome P450 gene, CYP321E1 from the diamondback moth, Plutella xylostella (L.) and RNA interference to evaluate its role in chlorantraniliprole resistance.
abstract: Insect cytochrome P450 monooxygenases (P450s) play an important role in catalysis of many reactions leading to insecticides resistance. Our previous studies on transcriptome analysis of chlorantraniliprole-resistant development in the diamondback moth, Plutella xylostella revealed that up-regulation of cytochrome P450s are one of the main factors leading to the development of chlorantraniliprole resistance. Here, we report for the first time a novel cytochrome P450 gene CYP321E1, which belongs to the cytochrome P450 gene family CYP321. Real-time quantitative PCR (RT-qPCR) analyses indicated that CYP321E1 was expressed at all developmental stages of P. xylostella but was highest in the fourth-instar larvae; furthermore, the relatively high expression was observed in the midgut of the fourth-instar larvae, followed by fat bodies and epidermis. The expression of CYP321E1 in P. xylostella was differentially affected by three representative insecticides, including alphamethrin, abamectin and chlorantraniliprole. Among them, the exposure to chlorantraniliprole resulted in the largest transcript level of this cytochrome P450 gene. The findings suggested potential involvement of CYP321E1 in chlorantraniliprole resistance of P. xylostella. To assess the functional link of CYP321E1 to chlorantraniliprole resistance, RNA interference (RNAi)-mediated gene silencing by double stranded RNA (dsRNA) injecting was used. Results revealed that injection delivery of dsRNA can greatly reduce gene expression after 24 h. As a consequence of RNAi, a significant increment in mortality of larvae injected CYP321E1 dsRNA was observed after 24 h of exposure to chlorantraniliprole. These results strongly support our notion that this novel cytochrome P450 gene plays an important role in chlorantraniliprole detoxification in the diamondback moth and is partly responsible for its resistance.
authors.affiliation: Institute of Plant Protection,Guangdong Academy of Agricultural Sciences, Guangdong, Guangzhou 510640,People's Republic of China.
authors.forename: Z
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Institute of Plant Protection,Guangdong Academy of Agricultural Sciences, Guangdong, Guangzhou 510640,People's Republic of China.
authors.forename: Q
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Institute of Plant Protection,Guangdong Academy of Agricultural Sciences, Guangdong, Guangzhou 510640,People's Republic of China.
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.affiliation: Institute of Plant Protection,Guangdong Academy of Agricultural Sciences, Guangdong, Guangzhou 510640,People's Republic of China.
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Plant Protection,Guangdong Academy of Agricultural Sciences, Guangdong, Guangzhou 510640,People's Republic of China.
authors.forename: F
authors.initials: F
authors.lastname: Yin
authors.affiliation: Institute of Plant Protection,Guangdong Academy of Agricultural Sciences, Guangdong, Guangzhou 510640,People's Republic of China.
authors.forename: X
authors.initials: X
authors.lastname: Feng
mesh_terms: D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007305:Insecticide Resistance / Q000235:genetics*; D007306:Insecticides / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D009036:Moths / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D034622:RNA Interference; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D062367:ortho-Aminobenzoates; C517733:chlorantranilipole; D003577:Cytochrome P-450 Enzyme System