title: Toward larger DNA origami.
abstract: Structural DNA nanotechnology, and specifically scaffolded DNA origami, is rapidly developing as a versatile method for bottom-up fabrication of novel nanometer-scale materials and devices. However, lengths of conventional single-stranded scaffolds, for example, 7,249-nucleotide circular genomic DNA from the M13mp18 phage, limit the scales of these uniquely addressable structures. Additionally, increasing DNA origami size generates the cost burden of increased staple-strand synthesis. We addressed this 2-fold problem by developing the following methods: (1) production of the largest to-date biologically derived single-stranded scaffold using a λ/M13 hybrid virus to produce a 51 466-nucleotide DNA in a circular, single-stranded form and (2) inexpensive DNA synthesis via an inkjet-printing process on a chip embossed with functionalized micropillars made from cyclic olefin copolymer. We have experimentally demonstrated very efficient assembly of a 51-kilobasepair origami from the λ/M13 hybrid scaffold folded by chip-derived staple strands. In addition, we have demonstrated two-dimensional, asymmetric origami sheets with controlled global curvature such that they land on a substrate in predictable orientations that have been verified by atomic force microscopy.
authors.affiliation: Biomedical Engineering Department, Duke University , Durham, North Carolina 27708, United States.
authors.forename: Alexandria N
authors.initials: AN
authors.lastname: Marchi
authors.forename: Ishtiaq
authors.initials: I
authors.lastname: Saaem
authors.forename: Briana N
authors.initials: BN
authors.lastname: Vogen
authors.forename: Stanley
authors.initials: S
authors.lastname: Brown
authors.forename: Thomas H
authors.initials: TH
authors.lastname: LaBean
mesh_terms: D010582:Bacteriophage lambda / Q000737:chemistry; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D004279:DNA, Viral / Q000138:chemical synthesis / Q000737:chemistry; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation
chemical_list: D004279:DNA, Viral; D004247:DNA
keywords: DNA origami; Nanotechnology; hybrid bacteriophage; lambda DNA; on-chip DNA synthesis; structural DNA nanotechnology