title: Development and characterization of nine microsatellites for an endangered tree, Pinus wangii (Pinaceae).
abstract: OBJECTIVE
  Pinus wangii is an endemic and endangered species in southwestern China, and microsatellite primers were developed to characterize its genetic diversity and population structure. •
  
  
  RESULTS
  Using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, nine sets of microsatellite primers were developed in P. wangii. One population with 26 individuals of P. wangii, as well as 11 individuals each for two congeneric species, P. taiwanensis and P. squamata, were used to test their polymorphism and transferability. The number of alleles per locus ranged from one to seven with an average of 3.7, and the observed heterozygosity and expected heterozygosity ranged from 0 to 0.91 and 0 to 0.75, respectively. •
  
  
  CONCLUSIONS
  We developed nine sets of polymorphic microsatellite loci that are suitable for investigating genetic diversity and population structure of P. wangii, and these markers may be useful for other Pinus species.
authors.affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, People's Republic of China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Dou
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Ren-Chao
authors.initials: RC
authors.lastname: Zhou
authors.affiliation: College of Life Sciences, Chongqing Normal University, Chongqing 401331, People's Republic of China.
authors.forename: An-Jun
authors.initials: AJ
authors.lastname: Tang
authors.affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, People's Republic of China.
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Ge
authors.affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
keywords: Pinaceae; Pinus wangii; microsatellite; population genetics