title: Real-time PCR carried out on DNA extracted from serum or blood sample is not a good method for surveillance of bovine brucellosis.
abstract: Bovine brucellosis is endemic in many parts of the world including India. The disease diagnosis and surveillance are usually carried out by serological tests, which however have drawbacks. This study was undertaken to evaluate the potential of real-time PCR (RT-PCR) targeting bcsp31 gene for surveillance of bovine brucellosis. A total of 461 samples, which included 408 stored serum and 53 prospective blood samples, were used. It was found that 33 (7.15 %) samples were positive by RT-PCR, whereas 149 (32.32 %) and 132 (28.63 %) were positive by Rose Bengal plate test (RBPT) or standard agglutination test (STAT), respectively. The results of this study suggest that RT-PCR targeting bcsp31 gene carried out on DNA extracted from serum or blood may not be a suitable method for surveillance of brucellosis in bovines.
authors.affiliation: Division of Microbiology, Defence Research & Development Establishment, Jhansi Road, Gwalior, Madhya Pradesh, 474002, India.
authors.forename: Arvind
authors.initials: A
authors.lastname: Tiwari
authors.forename: Vijai
authors.initials: V
authors.lastname: Pal
authors.forename: Prachiti
authors.initials: P
authors.lastname: Afley
authors.forename: Deepak Kumar
authors.initials: DK
authors.lastname: Sharma
authors.forename: Chandra Shekhar
authors.initials: CS
authors.lastname: Bhatnagar
authors.forename: Bhupendra
authors.initials: B
authors.lastname: Bhardwaj
authors.forename: Ganga Prasad
authors.initials: GP
authors.lastname: Rai
authors.forename: Subodh
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D002002:Brucella / Q000235:genetics; D002007:Brucellosis, Bovine / Q000453:epidemiology*; D002417:Cattle; D004269:DNA, Bacterial / Q000097:blood* / Q000235:genetics; D007194:India / Q000453:epidemiology; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary*; D012395:Rose Bengal; D012698:Serologic Tests / Q000662:veterinary*
chemical_list: D004269:DNA, Bacterial; D012395:Rose Bengal