title: A ratiometric fluorescent probe based on ESIPT and AIE processes for alkaline phosphatase activity assay and visualization in living cells.
abstract: Alkaline phosphatase (ALP) activity is regarded as an important biomarker in medical diagnosis. A ratiometric fluorescent probe is developed based on a phosphorylated chalcone derivative for ALP activity assay and visualization in living cells. The probe is soluble in water and emits greenish-yellow in aqueous buffers. In the presence of ALP, the emission of probe changes to deep red gradually with ratiometric fluorescent response due to formation and aggregation of enzymatic product, whose fluorescence involves both excited-state intramolecular proton transfer and aggregation-induced emission processes. The linear ratiometric fluorescent response enables in vitro quantification of ALP activity in a range of 0-150 mU/mL with a detection limit of 0.15 mU/mL. The probe also shows excellent biocompatibility, which enables it to apply in ALP mapping in living cells.
authors.affiliation: HKUST Shenzhen Research Institute , No. 9 Yuexing First RD, South Area, Hi-Tech Park, Nanshan, Shenzhen, China 518057.
authors.forename: Zhegang
authors.initials: Z
authors.lastname: Song
authors.forename: Ryan T K
authors.initials: RT
authors.lastname: Kwok
authors.forename: Engui
authors.initials: E
authors.lastname: Zhao
authors.forename: Zikai
authors.initials: Z
authors.lastname: He
authors.forename: Yuning
authors.initials: Y
authors.lastname: Hong
authors.forename: Jacky W Y
authors.initials: JW
authors.lastname: Lam
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Ben Zhong
authors.initials: BZ
authors.lastname: Tang
mesh_terms: D000469:Alkaline Phosphatase / Q000276:immunology*; D002470:Cell Survival; D018360:Crystallography, X-Ray; D057075:Enzyme Assays / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D055668:Photochemical Processes; D011522:Protons; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D011522:Protons; D000469:Alkaline Phosphatase
keywords: ESIPT; aggregation-induced emission; alkaline phosphatase; bioprobe; ratiometric fluorescent probe