title: Evaluation of protective immune response in mice by vaccination the recombinant adenovirus for expressing Schistosoma japonicum inhibitor apoptosis protein.
abstract: Schistosomiasis is a worldwide parasitic disease, and while it can be successfully treated with chemotherapy, this does not prevent reinfection with the parasite. Adenovirus vectors have been widely used for vaccine delivery, and a vaccination approach has the potential to prevent infection with Schistosoma. Here, we developed a recombinant adenoviral vector that expresses Schistosoma japonicum inhibitor apoptosis protein (Ad-SjIAP) and assessed its immunoprotective functions against schistosomiasis in mice. Murine immune responses following vaccination were investigated using enzyme-linked immunosorbent assays (ELISA), lymphocyte proliferation, and cytokine assays. The protective immunity in mice was evaluated by challenging with S. japonicum cercariae. Our results indicated that immunization with the Ad-SjIAP in mice induced a strong serum IgG response against IAP including IgG1, IgG2a, and IgG2b. In addition, lymphocyte proliferation experiments showed that mice treated with Ad-SjIAP significantly increased the lymphocyte response upon stimulation with recombinant Schistosoma japonicum inhibitor apoptosis protein (rSjIAP). Moreover, cytokine assays indicated that vaccination of Ad-SjIAP significantly increased the production of interferon (IFN)-γ and IL-2 as compared to the corresponding control group. Furthermore, following the challenge with S. japonicum cercariae, the vaccine conferred moderate protection, with an average rate of 37.95% for worm reduction and 31.7% for egg reduction. Taken together, our preliminarily results suggested that schistosoma IAP may be a potential vaccine against S. japonicum and that adenoviral vectors may serve as an alternative delivery vehicle for schistosome vaccine development.
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, 518 Ziyue Road, Shanghai, 200241, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Hu
authors.forename: Lihui
authors.initials: L
authors.lastname: Zhu
authors.forename: Rong
authors.initials: R
authors.lastname: Luo
authors.forename: Jinwei
authors.initials: J
authors.lastname: Dao
authors.forename: Jiangping
authors.initials: J
authors.lastname: Zhao
authors.forename: Yaojun
authors.initials: Y
authors.lastname: Shi
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Ke
authors.initials: K
authors.lastname: Lu
authors.forename: Xingang
authors.initials: X
authors.lastname: Feng
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Lin
authors.forename: Jinming
authors.initials: J
authors.lastname: Liu
authors.forename: Guofeng
authors.initials: G
authors.lastname: Cheng
mesh_terms: D000256:Adenoviridae; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D015801:Helminth Proteins / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D051034:Inhibitor of Apoptosis Proteins / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008807:Mice, Inbred BALB C; D010270:Parasite Egg Count; D012549:Schistosoma japonicum; D012554:Schistosomiasis japonica / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D015801:Helminth Proteins; D007074:Immunoglobulin G; D051034:Inhibitor of Apoptosis Proteins; D007376:Interleukin-2; D014614:Vaccines, Synthetic; D007371:Interferon-gamma