title: Recombination in the evolution of human bocavirus.
abstract: Whole genome sequencing of Novosibirsk human bocavirus (HBoV) isolates has detected an isolate that emerged via recombination between HBoV3 and HBoV4 genotypes. The recombination site is located between regions with abnormally low and abnormally high GC contents in the genome. This site is a bocavirus recombination hotspot and coincides with one of two parvovirus recombination hotspots. The Novosibirsk recombinant isolate, which is similar to a previously studied isolate from Thailand, utilizes the strategy of borrowing ORF3, which encodes structural proteins, of a rare genotype HBoV4. The role of recombination in HBoV evolution is discussed.
authors.affiliation: Laboratory of Molecular Microbiology, Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia.
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Tyumentsev
authors.affiliation: Laboratory of Molecular Microbiology, Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia.
authors.forename: Nina V
authors.initials: NV
authors.lastname: Tikunova
authors.affiliation: Laboratory of Molecular Microbiology, Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia.
authors.forename: Artem Yu
authors.initials: AY
authors.lastname: Tikunov
authors.affiliation: Laboratory of Molecular Microbiology, Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia. Electronic address: i_babkin@mail.ru.
authors.forename: Igor V
authors.initials: IV
authors.lastname: Babkin
mesh_terms: D001482:Base Composition; D019143:Evolution, Molecular; D016679:Genome, Viral; D005838:Genotype; D056661:Human bocavirus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007223:Infant; D008297:Male; D016366:Open Reading Frames; D010322:Parvoviridae Infections / Q000821:virology*; D016865:Reassortant Viruses; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
keywords: Complete genome; Human bocavirus; Recombination; Virus evolution