title: Study of the protective mechanisms of Compound Danshen Tablet (Fufang Danshen Pian) against myocardial ischemia/reperfusion injury via the Akt-eNOS signaling pathway in rats.
abstract: BACKGROUND
  Compound Danshen Tablet (CDT), an herbal preparation consisting of Salvia miltiorrhiza (Radix and rhizome of Salvia miltiorrhiza Bge.), Notoginseng (Radix and rhizome of Panax notoginseng (Burk.) F. H. Chen) and Borneolum Syntheticum, is widely used to improve coronary heart disease, cardiac angina and atherosclerosis in clinic in Asia and Pacific Ocean area, especially in China.
  
  
  OBJECTIVE
  The study is to research the protective mechanisms of Compound Danshen Tablet (CDT) against myocardial ischemia/reperfusion (MI/R) injury via the Akt-eNOS signaling Pathway in rats.
  
  
  METHODS
  Rats were randomized into 7 groups: Sham group; Model group; Low-Dose CDT group (0.315 g/kg/d, i.g); Middle-Dose CDT group (0.63 g/kg/d, i.g); High-Dose CDT group (1.26 g/kg/d, i.g); Compound Danshen Dripping Pills (CDDP) group (0.0945 g/kg/d, i.g); Sulfotanshinone Sodium Injection (Tan II A) group (5 mg/kg/d, i.m). After the administration, the hearts of the rats were subjected to 30 min of coronary artery occlusion and 2h of reperfusion except the Sham group rats.
  
  
  RESULTS
  CDT significantly decreased infarct size, apoptosis, caspase-3 protein expression, MDA level in myocardial tissues, the activities of serum CK, LDH and cTnI; on the contrary, it increased p-Akt, p-eNOS, bcl-2 protein expression, the activities of SOD and tissue LDH, and the level of NO.
  
  
  CONCLUSIONS
  CDT can protect cardiomyocytes against MI/R injury and inhibits apoptosis in rats by activating Akt-eNOS signaling pathway.
authors.affiliation: Guangzhou General Medicine & Pharmaceutical Research Institute, Guangzhou, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Ren-an
authors.affiliation: Jinan University College of Pharmacy, Guangzhou 510632, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Juan
authors.affiliation: Guangzhou General Medicine & Pharmaceutical Research Institute, Guangzhou, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Chuyuan
authors.affiliation: Jinan University College of Pharmacy, Guangzhou 510632, PR China.
authors.forename: Fu
authors.initials: F
authors.lastname: Wenjuan
authors.affiliation: Jinan University College of Pharmacy, Guangzhou 510632, PR China.
authors.forename: Huang
authors.initials: H
authors.lastname: Chunyan
authors.affiliation: Jinan University College of Pharmacy, Guangzhou 510632, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xuemei
authors.affiliation: Guangzhou General Medicine & Pharmaceutical Research Institute, Guangzhou, PR China.
authors.forename: Huang
authors.initials: H
authors.lastname: Lin
authors.affiliation: Jinan University College of Pharmacy, Guangzhou 510632, PR China. Electronic address: hongnie1970@163.com.
authors.forename: Nie
authors.initials: N
authors.lastname: Hong
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D053723:Panax notoginseng / Q000737:chemistry; D020011:Protective Agents / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects; D013607:Tablets / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D020011:Protective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D013607:Tablets; C517866:fufang danshen; C028972:dan-shen root extract; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; D051057:Proto-Oncogene Proteins c-akt; D053148:Caspase 3
keywords: Akt; Compound Danshen Tablet; Myocardial ischemia/reperfusion injury; Protective Mechanisms; Signaling pathway; eNOS