title: Rapid identification of traditional Chinese herbal medicine by direct analysis in real time (DART) mass spectrometry.
abstract: Direct analysis in real time-mass spectrometry (DART-MS) was employed as a novel fast method to identify traditional Chinese herbal medicine (TCHM). In order to obtain high quality mass spectra, the ionization temperature was optimized for every kind of sample. With minimal or no sample pretreatment, major TCHM components, including alkaloids, flavonoids and some ginsenosides, were directly detected within several seconds, while thirteen ginsenosides need derivatization to get good mass spectra. Pseudoginsenoside F11, compound K, protopanaxatriol (PPT) and protopanaxadiol (PPD), for the first time were detected without derivatization. Among five of eight tested Chinese herbal medicines, Rhizoma Corydalis, Bulbus Fritillariae Thunbergii, Arecae Semen, Ramulus Uncariae Cum Uncis and Scutellariae Radix, were first time identified by DART-MS. In addition, the ionization mechanisms of major herbal components, alkaloids, flavonoids and ginsenosides, were discussed in detail. Our results demonstrated that DART-MS could provide a rapid, reliable and environmental friendly method for the rapid identification of TCHM, and may be applicable to other plants.
authors.affiliation: Jilin Ginseng Academy, Changchun University of Chinese Medicine, Jilin, Changchun 130117, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Jilin Ginseng Academy, Changchun University of Chinese Medicine, Jilin, Changchun 130117, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Li
authors.affiliation: Jilin Ginseng Academy, Changchun University of Chinese Medicine, Jilin, Changchun 130117, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Huang
authors.affiliation: School of Pharmaceutical, Changchun University of Chinese Medicine, Jilin, Changchun 130117, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Jilin Ginseng Academy, Changchun University of Chinese Medicine, Jilin, Changchun 130117, China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Guo
authors.affiliation: School of Environmental Air Security and Pollution Control Engineering, Jinan University, Guangzhou 510632, China. Electronic address: mali304@yahoo.com.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.affiliation: Jilin Ginseng Academy, Changchun University of Chinese Medicine, Jilin, Changchun 130117, China. Electronic address: syliu@ciac.ac.cn.
authors.forename: Shuying
authors.initials: S
authors.lastname: Liu
mesh_terms: D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry*; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D013696:Temperature; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Alkaloids; Direct analysis in real time (DART); Flavonoids; Ginsenosides; Time-of-flight mass spectrometry