title: [Screening bioactive compounds inhibiting influenza virus from isatidis radix by ultrafiltration mass spectrometry].
abstract: In vitro neuraminidase inhibition assays and ultrafiltration liquid chromatography with diodearray detector coupled to time of flight mass spectrometer (UPLC-DAD-TOF-MS) were combined to screen bioactive compounds inhibiting neuraminidase from Isatidis Radix. By comparing the compounds from Isatidis Radix before and after ultrafiltration, we found that arginine, goitrin and adenosinea can bind with neuraminidase, and the binding degree of the three compounds were (36.23 +/- 1.12)%, (32.54 +/- 1.02)% and (9.38 +/- 0.47)%, respectively. The IC50 of arginine and goitrin were (1.16 +/- 0.02), (1.20 +/- 0.02) g x L(-1), respectively. While the IC50 of adenosinea was higher than 500 g x L(-1). The results showed that arginine and goitrin might be the main compounds with antiviral activity of Isatidis Radix. This study may provide a useful method for the screening of bioactive compounds and quality control of Isatidis Radix.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Ma
authors.forename: Cong-En
authors.initials: CE
authors.lastname: Zhang
authors.forename: Dan
authors.initials: D
authors.lastname: Yan
authors.forename: Man-Rong
authors.initials: MR
authors.lastname: Tan
authors.forename: Han-Bing
authors.initials: HB
authors.lastname: Li
authors.forename: Le-Le
authors.initials: LL
authors.lastname: Zhang
authors.forename: Yin
authors.initials: Y
authors.lastname: Xiong
authors.forename: Xiao-He
authors.initials: XH
authors.lastname: Xiao
mesh_terms: D000998:Antiviral Agents / Q000032:analysis / Q000494:pharmacology*; D001120:Arginine / Q000032:analysis / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000494:pharmacology*; D031219:Isatis / Q000737:chemistry*; D013058:Mass Spectrometry; D009439:Neuraminidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D009975:Orthomyxoviridae / Q000187:drug effects* / Q000201:enzymology; D023303:Oxazolidinones / Q000032:analysis / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*; D014462:Ultrafiltration; D014764:Viral Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D023303:Oxazolidinones; D014764:Viral Proteins; C004333:goitrin; D001120:Arginine; D009439:Neuraminidase