title: Lin28B is a novel prognostic marker in gastric adenocarcinoma.
abstract: Lin28B, a homologue of Lin28, represses biogenesis of let-7 microRNAs with a role in tumorigenesis and is considered a potential therapeutic target for various human cancers. The aim of the study was to identify the clinical significance of Lin28B in gastric adenocarcinoma (GAC). We examined the expression of Lin28B in 97 human gastric cancer samples with 32 samples of non-dysplastic tissues by immunohistochemistry. In the 97 GAC cases, 42 were with high Lin28B expression. The expression levels of Lin28B proteins in GAC were higher than in corresponding adjacent normal tissues (P=0.001). Significant correlations were noted between Lin28B expression and lymph node status (P=0.005), TNM stage (P < 0.001), tumor invasion (P=0.036), and differentiation (P=0.001) of GAC patients. The Kaplan-Meier estimates showed a negative correlation of overall 5-year survival rate with Lin28B expression where higher expression resulted in poorer prognosis in GAC. In univariate analysis, lymph node metastasis, TNM stage, serosal invasion, Lin28B expression as well as differentiation grade could predict the prognosis of GAC patients (P=0.002, P < 0.001, P=0.003, P < 0.001, P=0.001, respectively). Multivariate analysis revealed that the expression level of Lin28B (P < 0.001), TNM stage (P < 0.001) as well as differentiation grade (P < 0.001) were the three potential independent prognostic factors in our study [Hazard ratio (HR)=2.108 and P=0.017, HR=1.994 and P=0.018, HR=1.939 and P=0.046, respectively]. Our findings point to the prognostic role of Lin28B in GAC, and indicate Lin28B as a potential therapeutic target of GAC patients.
authors.affiliation: Laboratory of Genetics, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University Chengdu 610041, China ; Laboratory of Cell and Gene Therapy, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University Chengdu 610041, China ; Department of Obstetric and Gynecologic, West China Second University Hospital, Sichuan University Chengdu 610041, China ; Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education Chengdu 610041, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Laboratory of Genetics, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University Chengdu 610041, China ; Laboratory of Cell and Gene Therapy, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University Chengdu 610041, China ; Department of Obstetric and Gynecologic, West China Second University Hospital, Sichuan University Chengdu 610041, China ; Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education Chengdu 610041, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Peng
authors.affiliation: Department of Pathology, West China Hospital of Sichuan University 37 Guoxue Street, Chengdu, Sichuan 610041, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Obstetric and Gynecologic, Henan Provincial People's Hospital Zhengzhou, Henan 450003, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Gynecological Oncology, Second People's Hospital of Sichuan (Sichuan Cancer Hospital) Chengdu, Sichuan 610041, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Cui
authors.affiliation: Laboratory of Genetics, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University Chengdu 610041, China ; Laboratory of Cell and Gene Therapy, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University Chengdu 610041, China ; Department of Obstetric and Gynecologic, West China Second University Hospital, Sichuan University Chengdu 610041, China ; Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education Chengdu 610041, China.
authors.forename: Xinlian
authors.initials: X
authors.lastname: Chen
authors.affiliation: Laboratory of Genetics, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University Chengdu 610041, China ; Laboratory of Cell and Gene Therapy, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University Chengdu 610041, China ; Department of Obstetric and Gynecologic, West China Second University Hospital, Sichuan University Chengdu 610041, China ; Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education Chengdu 610041, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yang
authors.affiliation: Laboratory of Genetics, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University Chengdu 610041, China ; Laboratory of Cell and Gene Therapy, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University Chengdu 610041, China ; Department of Obstetric and Gynecologic, West China Second University Hospital, Sichuan University Chengdu 610041, China ; Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education Chengdu 610041, China.
authors.forename: Hongqian
authors.initials: H
authors.lastname: Liu
authors.affiliation: Laboratory of Cell and Gene Therapy, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University Chengdu 610041, China ; Department of Obstetric and Gynecologic, West China Second University Hospital, Sichuan University Chengdu 610041, China ; Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education Chengdu 610041, China.
authors.forename: Shanling
authors.initials: S
authors.lastname: Liu
authors.affiliation: Laboratory of Genetics, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University Chengdu 610041, China ; Department of Obstetric and Gynecologic, West China Second University Hospital, Sichuan University Chengdu 610041, China ; Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education Chengdu 610041, China.
authors.forename: He
authors.initials: H
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D016601:RNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis*; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; C516326:LIN28B protein, human; D016601:RNA-Binding Proteins
keywords: Lin28B expression; gastric adenocarcinoma; multivariate Cox proportional hazards model analysis; overall 5-year survival rate; prognosis