title: Residual pattern of fenhexamid on pepper fruits grown under greenhouse conditions using HPLC and confirmation via tandem mass spectrometry.
abstract: Fenhexamid (25%, SC) was sprayed on pepper fruits grown under greenhouse conditions at the recommended dose rate of 20g/20L water. Fruit samples were collected randomly at 0 (2h after application), 1, 2, 4, 6, 8, 11, and 14days post-application. The samples were extracted with acetonitrile, partitioned with water, passed through a cleanup procedure, and analysed via HPLC. Residues were confirmed via LC-tandem mass spectrometry (LC-MS/MS) in positive-ion electrospray ionisation (ESI+) mode. The rate of disappearance of fenhexamid on pepper fruits was described as first-order kinetics (r(2)=0.992) with a half-life of 4.7-day. Based on the pattern of decline of the fungicide residues in relation to the estimated maximum residue limits (MRL=5mg/kg), a safety pre-harvest interval of 1day is suggested for peppers at the recommended dosage.
authors.affiliation: Natural Products Chemistry Laboratory, College of Agriculture and Life Science, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea.
authors.forename: Lina
authors.initials: L
authors.lastname: Hem
authors.affiliation: Natural Products Chemistry Laboratory, College of Agriculture and Life Science, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea.
authors.forename: Jeong-Heui
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Natural Products Chemistry Laboratory, College of Agriculture and Life Science, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea.
authors.forename: Jong-Hyouk
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Chemistry, University of Dhaka, Dhaka 1000, Bangladesh.
authors.forename: Md I R
authors.initials: MI
authors.lastname: Mamun
authors.affiliation: National Agricultural Products Quality Management Service, Products Safety Inspection Laboratory, Gwangsan-gu, Gwangju, Republic of Korea.
authors.forename: Soon-Kil
authors.initials: SK
authors.lastname: Cho
authors.affiliation: Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt. Electronic address: abdelaty44@hotmail.com.
authors.forename: A M
authors.initials: AM
authors.lastname: Abd El-Aty
authors.affiliation: Natural Products Chemistry Laboratory, College of Agriculture and Life Science, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea. Electronic address: jhshim@chonnam.ac.kr.
authors.forename: Jae-Han
authors.initials: JH
authors.lastname: Shim
keywords: Disappearance pattern; Fenhexamid; Pepper fruits; Pre-harvest interval; Residue analysis