title: [Special application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry in clinical microbiological diagnostics].
abstract: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry as a new possibility for rapid identification of bacteria and fungi revolutionized the clinical microbiological diagnostics. It has an extreme importance in the routine microbiological laboratories, as identification of the pathogenic species rapidly will influence antibiotic selection before the final determination of antibiotic resistance of the isolate. The classical methods for identification of bacteria or fungi, based on biochemical tests, are influenced by many environmental factors. The matrix-assisted laser desorption ionization time-of-flight mass spectrometry is a rapid method which is able to identify a great variety of the isolated bacteria and fungi based on the composition of conserved ribosomal proteins. Recently several other applications of the method have also been investigated such as direct identification of pathogens from the positive blood cultures. There are possibilities to identify bacteria from the urine samples in urinary tract infection or from other sterile body fluids. Using selective enrichment broth Salmonella sp from the stool samples can be identified more rapidly, too. The extended spectrum beta-lactamase or carbapenemase production of the isolated bacteria can be also detected by this method helping the antibiotic selection in some cases. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry based methods are suitable to investigate changes in deoxyribonucleic acid or ribonucleic acid, to carry out rapid antibiotic resistance determination or other proteomic analysis. The aim of this paper is to give an overview about present possibilities of using this technique in the clinical microbiological routine procedures.
authors.affiliation: Szegedi Tudományegyetem, Általános Orvostudományi Kar Klinikai Mikrobiológiai Diagnosztikai Intézet Szeged Pf. 427 6701.
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Nagy
authors.affiliation: Szegedi Tudományegyetem, Általános Orvostudományi Kar Klinikai Mikrobiológiai Diagnosztikai Intézet Szeged Pf. 427 6701.
authors.forename: Marianna
authors.initials: M
authors.lastname: Abrók
authors.affiliation: Szegedi Tudományegyetem, Általános Orvostudományi Kar Klinikai Mikrobiológiai Diagnosztikai Intézet Szeged Pf. 427 6701.
authors.forename: Noémi
authors.initials: N
authors.lastname: Bartha
authors.affiliation: Szegedi Tudományegyetem, Általános Orvostudományi Kar Klinikai Mikrobiológiai Diagnosztikai Intézet Szeged Pf. 427 6701.
authors.forename: László
authors.initials: L
authors.lastname: Bereczki
authors.affiliation: Semmelweis Egyetem, Általános Orvostudományi Kar Laboratóriumi Medicina Intézet, Klinikai Mikrobiológiai Diagnosztikai Laboratórium Budapest.
authors.forename: Emese
authors.initials: E
authors.lastname: Juhász
authors.affiliation: Debreceni Egyetem, Általános Orvostudományi Kar Orvosi Mikrobiológiai Intézet Debrecen.
authors.forename: Gábor
authors.initials: G
authors.lastname: Kardos
authors.affiliation: Semmelweis Egyetem, Általános Orvostudományi Kar Laboratóriumi Medicina Intézet, Klinikai Mikrobiológiai Diagnosztikai Laboratórium Budapest.
authors.forename: Katalin
authors.initials: K
authors.lastname: Kristóf
authors.affiliation: Debreceni Egyetem, Általános Orvostudományi Kar Orvosi Mikrobiológiai Intézet Debrecen.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Miszti
authors.affiliation: Szegedi Tudományegyetem, Általános Orvostudományi Kar Klinikai Mikrobiológiai Diagnosztikai Intézet Szeged Pf. 427 6701.
authors.forename: Edit
authors.initials: E
authors.lastname: Urbán
mesh_terms: D016470:Bacteremia / Q000175:diagnosis; D001419:Bacteria / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification; D001424:Bacterial Infections / Q000175:diagnosis* / Q000188:drug therapy; D001426:Bacterial Proteins / Q000096:biosynthesis; D004352:Drug Resistance, Microbial; D016469:Fungemia / Q000175:diagnosis; D006801:Humans; D009181:Mycoses / Q000175:diagnosis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000706:statistics & numerical data*; D014552:Urinary Tract Infections / Q000175:diagnosis; D001618:beta-Lactamases / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase
keywords: MALDI-TOF MS; antibiotic resistance determination; rezisztenciameghatározás; species level identification; speciesidentifikálás; tipizálás; typing