title: New steroidal glycosides from the rhizome of Anemarrhena asphodeloides.
abstract: Two new steroidal saponins, timosaponin X (1) and timosaponin Y (2), and one new pregnane glycoside, timopregnane B (3), were isolated from the rhizomes of Anemarrhena asphodeloides, as well as three known compounds 25S-timosaponin BII (4), protodesgalactotigonin (5), and timosaponin BII-a (6) isolated from this plant for the first time. By the detailed analysis of 1D, 2D NMR, MS spectra, and chemical evidences, the structures of new compounds were elucidated as 26-O-β-d-glucopyranosyl-(25S)-5β-22-methoxy-furost-3β,26-diol 3-O-β-d-glucopyranosyl-(1 → 2)-α-l-arabinopyranoside (1), 5β-pseudo-spirost-3β,15α,23α-triol 3-O-β-d-glucopyranosyl-(1 → 2)-β-d-galactopyranoside (2), (5β,17α)-Δ((16)(17))-20-one-pregn-2β,3β-diol 3-O-β-d-glucopyranosyl-(1 → 2)-β-d-galactopyranoside (3).
authors.affiliation: a Department of Biotechnology , Beijing Institute of Radiation Medicine , Beijing 100850 , China.
authors.forename: Jian-Chao
authors.initials: JC
authors.lastname: Yuan
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Fang-Xu
authors.initials: FX
authors.lastname: Wang
authors.forename: Xu
authors.initials: X
authors.lastname: Pang
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Cheng-Qi
authors.initials: CQ
authors.lastname: Xiong
authors.forename: Bai-Ping
authors.initials: BP
authors.lastname: Ma
mesh_terms: D031403:Anemarrhena / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011278:Pregnanes / Q000737:chemistry / Q000302:isolation & purification*; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D004365:Drugs, Chinese Herbal; D006027:Glycosides; D011278:Pregnanes; D012503:Saponins; D013256:Steroids; C574437:pregnane glycoside; C000593147:timopregnane B; C523027:timosaponin B-II; C000593149:timosaponin Y
keywords: Anemarrhena asphodeloides; NMR; pregnane glycoside; steroidal saponin