title: Development of an appropriate PCR system for the reclassification of Streptococcus suis.
abstract: Thirty-five serotypes of Streptococcus suis (serotypes 1-34 and serotype 1/2) have so far been described on the basis of their polysaccharide capsular antigens. However, in the last decade, some serotype reference strains have been reexamined for their taxonomic status, and the reference strains of serotypes 20, 22, 26, 32, 33, and 34 may be different from taxon S. suis. In the present study, we developed a novel PCR method targeting the recombination/repair protein (recN) gene of S. suis, designated recN PCR, which corresponds to the current reclassification of this bacterium. We compared its specificity with other PCR methods for S. suis, and the results obtained confirmed its specificity. In addition, the detection limits of recN PCR were similar among all the reference strains of authentic S. suis, indicating that the recN PCR gave reliable results against bacterial strains and isolates used in this study. Therefore, recN PCR described in the present study will be a useful tool for the identification of authentic S. suis, and can also be used in epidemiological studies on this bacterium.
authors.affiliation: Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Sakura
authors.initials: S
authors.lastname: Ishida
authors.affiliation: Department of Biotechnology, Nong Lam University, Quarter 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam.
authors.forename: Le Hong Thuy
authors.initials: le HT
authors.lastname: Tien
authors.affiliation: Department of Bioresource Sciences, Graduate School of Agricultural Sciences, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe, Hyogo 657-8501, Japan.
authors.forename: Ro
authors.initials: R
authors.lastname: Osawa
authors.affiliation: Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Tohya
authors.affiliation: Organization for Advanced Science and Technology, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe, Hyogo 657-8501, Japan.
authors.forename: Ryohei
authors.initials: R
authors.lastname: Nomoto
authors.affiliation: Department of Microbiology, Aichi Gakuin University, School of Pharmacy, 1-100 Kusumoto-cho, Nagoya, Aichi 464-8650, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kawamura
authors.affiliation: Department of Epizootiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: Tatsuhumi
authors.initials: T
authors.lastname: Takahashi
authors.affiliation: Department of Epizootiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Kikuchi
authors.affiliation: Department of Infection Control Science and Department of Bacteriology, Faculty of Medicine, Juntendo University, Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Kikuchi
authors.affiliation: Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan. Electronic address: asekizak@mail.ecc.u-tokyo.ac.jp.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Sekizaki
mesh_terms: D000818:Animals; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000662:veterinary; D017011:Streptococcus suis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial
keywords: Diagnosis; PCR assay; Reclassification; Streptococcus suis; recN gene