title: The value of anti-desmoglein enzyme-linked immunosorbent assay in the immunological follow-up of pemphigus.
abstract: BACKGROUND
  The value of anti-desmoglein 1 and 3 (Dsg1, Dsg3) enzyme-linked immunosorbent assay (ELISA) is controversial in the follow-up of pemphigus.
  
  
  OBJECTIVE
  To evaluate anti-desmoglein ELISA (Dsg ELISA) in the follow-up of pemphigus and compare ELISA with direct and indirect immunofluorescence in complete remission (CR).
  
  
  METHODS
  We performed a retrospective monocenter study of patients with pemphigus and consecutive sera samples collected at baseline (M0), 12 months (M12) and 24 or 36 months after M0 (M24/36). Tests were compared in CR and in active disease. Direct immunofluorescence and circulating autoantibodies were compared for patients with stable CR.
  
  
  RESULTS
  We included 36 patients. At M12, ELISA values did not differ between CR and active disease. At M24/36, Dsg3 but not Dsg1 ELISA values were lower in CR (p = 0.07). For 5/8 patients with stable CR, direct immunofluorescence and ELISA findings remained positive.
  
  
  CONCLUSIONS
  In routine practice, Dsg ELISA seems to be of little interest for immunological follow-up of pemphigus.
authors.affiliation: Department of Dermatology, Hôpitaux Universitaires Henri Mondor, Créteil, France.
authors.forename: Nathalia
authors.initials: N
authors.lastname: Bellon
authors.forename: Chantal
authors.initials: C
authors.lastname: André
authors.forename: Emilie
authors.initials: E
authors.lastname: Sbidian
authors.forename: Nicolas
authors.initials: N
authors.lastname: Ortonne
authors.forename: Pierre
authors.initials: P
authors.lastname: Wolkenstein
authors.forename: Olivier
authors.initials: O
authors.lastname: Chosidow
authors.forename: Saskia
authors.initials: S
authors.lastname: Ingen-Housz-Oro
mesh_terms: D000328:Adult; D000368:Aged; D016208:Databases, Factual; D051183:Desmoglein 1 / Q000032:analysis* / Q000097:blood*; D051184:Desmoglein 3 / Q000097:blood*; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D005500:Follow-Up Studies; D005602:France; D006801:Humans; D008297:Male; D008875:Middle Aged; D008991:Monitoring, Physiologic / Q000379:methods*; D010392:Pemphigus / Q000097:blood* / Q000503:physiopathology*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D018709:Statistics, Nonparametric; D055815:Young Adult
chemical_list: D051183:Desmoglein 1; D051184:Desmoglein 3