title: Dynamic culture of a thermosensitive collagen hydrogel as an extracellular matrix improves the construction of tissue-engineered peripheral nerve.
abstract: Tissue engineering technologies offer new treatment strategies for the repair of peripheral nerve injury, but cell loss between seeding and adhesion to the scaffold remains inevitable. A thermosensitive collagen hydrogel was used as an extracellular matrix in this study and combined with bone marrow mesenchymal stem cells to construct tissue-engineered peripheral nerve composites in vitro. Dynamic culture was performed at an oscillating frequency of 0.5 Hz and 35° swing angle above and below the horizontal plane. The results demonstrated that bone marrow mesenchymal stem cells formed membrane-like structures around the poly-L-lactic acid scaffolds and exhibited regular alignment on the composite surface. Collagen was used to fill in the pores, and seeded cells adhered onto the poly-L-lactic acid fibers. The DNA content of the bone marrow mesenchymal stem cells was higher in the composites constructed with a thermosensitive collagen hydrogel compared with that in collagen I scaffold controls. The cellular DNA content was also higher in the thermosensitive collagen hydrogel composites constructed with the thermosensitive collagen hydrogel in dynamic culture than that in static culture. These results indicate that tissue-engineered composites formed with thermosensitive collagen hydrogel in dynamic culture can maintain larger numbers of seeded cells by avoiding cell loss during the initial adhesion stage. Moreover, seeded cells were distributed throughout the material.
authors.affiliation: Department of Joint Surgery, Orthopedics Hospital of the Second Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Lanfeng
authors.initials: L
authors.lastname: Huang
authors.affiliation: Centre of Hand & Foot Surgery and Reparative & Reconstructive Surgery, Orthopedics Hospital of the Second Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Orthopedics Surgery, the Third Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Wanguo
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Joint Surgery, Orthopedics Hospital of the Second Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Dai
authors.affiliation: Institute of Orthopedics, the Second Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Zhenwu
authors.initials: Z
authors.lastname: Du
authors.affiliation: Department of Joint Surgery, Orthopedics Hospital of the Second Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Joint Surgery, Orthopedics Hospital of the Second Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Jianchao
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Ophthalmology, the Second Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Zhao
keywords: NSFC grant; biomaterials; bone marrow mesenchymal stem cells; dynamic culture; extracellular matrix; nerve regeneration; nerve scaffold; neural regeneration; peripheral nerve; poly-L-lactic acid; thermosensitive collagen hydrogel; tissue engineering