title: Identification of low-expressing transcripts of the NPY receptors' family in the murine lingual epithelia.
abstract: Detection of low-expressing transcripts in tissues is a major technical challenge that requires considerate advance planning. To produce high-quality publishable data, many controls need to be employed, including knock out animal models, independent assays, and high-end imaging techniques. The current protocol describes the use of commercial kit, QuantiGene ViewRNA 1-plex assay for a reliable detection of low-expressing transcripts in formalin-fixed paraffin-embedded murine tissues. Examples of positive (high-expressing) and negative (knock out) control samples are provided to describe a case study.
authors.affiliation: Division of Cell and Molecular Therapy, Cancer and Genetics Research Complex, University of Florida, 2033 Mowry Road, 103610, Gainesville, FL, 32610, USA, szlt@ufl.edu.
authors.forename: Sergei
authors.initials: S
authors.lastname: Zolotukhin
mesh_terms: D000818:Animals; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005557:Formaldehyde / Q000737:chemistry; D017403:In Situ Hybridization / Q000379:methods*; D051379:Mice; D018345:Mice, Knockout; D008853:Microscopy / Q000379:methods; D016612:Paraffin Embedding / Q000379:methods; D012313:RNA / Q000032:analysis*; D016707:Tissue Fixation / Q000379:methods; D014158:Transcription, Genetic
chemical_list: D005557:Formaldehyde; D012313:RNA