title: Development of a mouse model of supraspinatus tendon insertion site healing.
abstract: Supraspinatus (SS) tendon tears are common musculoskeletal injuries whose surgical repair exhibits the highest incidence of re-tear of any tendon. Development of therapeutics for improving SS tendon healing is impaired by the lack of a model that allows biological perturbations to identify mechanisms that underlie ineffective healing. The objective of this study was to develop a mouse model of supraspinatus insertion site healing by creating a reproducible SS tendon detachment and surgical repair which can be applied to a wide array of inbred mouse strains and genetic mutants. Anatomical and structural analyses confirmed that the rotator cuff of the mouse is similar to that of human, including the presence of a coracoacromial (CA) arch and an insertion site that exhibits a fibrocartilagenous transition zone. The surgical repair was successfully conducted on seven strains of mice that are commonly used in Orthopaedic Research suggesting that the procedure can be applied to most inbred strains and genetic mutants. The quality of the repair was confirmed with histology through 14 days after surgery in two mouse strains that represent the variation in mouse strains evaluated. The developed mouse model will allow us to investigate mechanisms involved in insertion site healing.
authors.affiliation: Leni and Peter W. May Department of Orthopaedics, Icahn School of Medicine at Mount Sinai, New York, 10029, New York.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Bell
authors.forename: Peter
authors.initials: P
authors.lastname: Taub
authors.forename: Paul
authors.initials: P
authors.lastname: Cagle
authors.forename: Evan L
authors.initials: EL
authors.lastname: Flatow
authors.forename: Nelly
authors.initials: N
authors.lastname: Andarawis-Puri
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D006811:Humerus / Q000503:physiopathology / Q000601:surgery; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D019463:Mice, Inbred MRL lpr; D016688:Mice, Inbred NOD; D023421:Models, Animal; D019637:Orthopedic Procedures / Q000379:methods; D019651:Plastic Surgery Procedures / Q000379:methods; D017006:Rotator Cuff / Q000503:physiopathology / Q000601:surgery; D013708:Tendon Injuries / Q000503:physiopathology* / Q000601:surgery*; D013710:Tendons / Q000503:physiopathology* / Q000601:surgery*; D014945:Wound Healing / Q000502:physiology*
keywords: murine; rotator cuff; supraspinatus healing; tendon repair