title: Proteomic analysis of human nasal mucosa: different expression profile in rhino-pathologic states.
abstract: BACKGROUND
  Rhinitis comprises several diseases with varying causes and different clinical manifestations and pathological features, but treated as a single clinical disorder. As heterogeneous disease, proper differential diagnosis is useful to delineate appropriate therapeutic intervention. Comparative proteomic investigation was aimed to provide information for specific differentially expressed proteins in rhino pathologic state, that could be used for diagnostic purpose and therapeutic monitoring.
  
  
  METHODS
  Proteins extracted from nasal mucosa cells of patients with different features of rhinitis and from control subjects, were separated by 2-DE. Proteins differentially expressed were identified by mass spectrometry (MS).
  
  
  RESULTS
  Comparative proteomic analyses led to the identification of eighteen proteins differentially expressed in patients with rhinitis, mainly related to cell defense and innate and acquired immunity. From that, at least one protein can be a possible candidate as biomarker of disease.
authors.affiliation: Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Gelardi
authors.affiliation: Institute of Food Sciences, Italian National Research Council (CNR), Avellino, Italy.
authors.forename: R A
authors.initials: RA
authors.lastname: Siciliano
authors.affiliation: Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Papa
authors.affiliation: Institute of Food Sciences, Italian National Research Council (CNR), Avellino, Italy.
authors.forename: M F
authors.initials: MF
authors.lastname: Mazzeo
authors.affiliation: Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari, Italy.
authors.forename: E
authors.initials: E
authors.lastname: De Nitto
authors.affiliation: Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Quaranta
authors.affiliation: Institute of Biomembranes and Bioenergetics (IBBE) National Research Council (CNR) c/o Department of Basic Medical Sciences Neurosciences and Sense Organs P.zza Giulio Cesare 11, 70124 Bari, Italy. E-mail: r.lippolis@ibbe.cnr.it.
authors.forename: R
authors.initials: R
authors.lastname: Lippolis
mesh_terms: D000328:Adult; D000444:Aldehyde Dehydrogenase / Q000276:immunology; D000080924:Aldehyde Dehydrogenase 1 Family; D000071396:Aldehyde Dehydrogenase, Mitochondrial / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology; D015415:Biomarkers; D015180:Electrophoresis, Gel, Two-Dimensional; D004804:Eosinophils / Q000473:pathology; D005260:Female; D051549:Glutathione S-Transferase pi / Q000276:immunology; D005982:Glutathione Transferase / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D055554:Hemoglobin Subunits / Q000276:immunology; D006801:Humans; D007527:Isoenzymes / Q000276:immunology; D008297:Male; D013058:Mass Spectrometry; D008407:Mast Cells / Q000473:pathology; D008875:Middle Aged; D009297:Nasal Mucosa / Q000276:immunology* / Q000473:pathology*; D009298:Nasal Polyps / Q000276:immunology / Q000473:pathology; D009504:Neutrophils / Q000473:pathology; D054464:Peroxiredoxins / Q000276:immunology; D010750:Phosphoproteins / Q000276:immunology; D040901:Proteomics; D050697:Retinal Dehydrogenase; D012220:Rhinitis / Q000235:genetics* / Q000276:immunology*; D009418:S100 Proteins / Q000276:immunology; D051137:Selenium-Binding Proteins / Q000276:immunology; D015843:Serpins / Q000276:immunology; D012709:Serum Albumin / Q000276:immunology; D013879:Thioredoxins / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; C485599:BPIFA1 protein, human; D015415:Biomarkers; D006023:Glycoproteins; D055554:Hemoglobin Subunits; D007527:Isoenzymes; D010750:Phosphoproteins; D009418:S100 Proteins; C495175:SELENBP1 protein, human; D051137:Selenium-Binding Proteins; D015843:Serpins; D012709:Serum Albumin; C051072:squamous cell carcinoma-related antigen; C072337:S100A11 protein, human; D013879:Thioredoxins; C515974:PRDX5 protein, human; D054464:Peroxiredoxins; D000080924:Aldehyde Dehydrogenase 1 Family; C096127:ALDH2 protein, human; C480021:ALDH3A1 protein, human; D000444:Aldehyde Dehydrogenase; D000071396:Aldehyde Dehydrogenase, Mitochondrial; C510223:ALDH1A1 protein, human; D050697:Retinal Dehydrogenase; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; C119995:glutathione S-transferase alpha
keywords: Human nasal mucosa; mass spectrometry; proteomics; rhinitis; two-dimensional electrophoresis