title: Paclitaxel loaded hyaluronic acid nanoparticles for targeted cancer therapy: in vitro and in vivo analysis.
abstract: The main aim of this work was to evaluate a nanoconjugate system of paclitaxel loaded self-assembling, biodegradable micelles for targeting CD44 overexpression in cancer cells. The shape and size, zeta potential, encapsulation efficiency and cell uptake of these drug-loaded micelles were evaluated. To understand their bio distribution profile, the hyaluronate (HA) micelles were labeled with Flamma™-774 NIR dye and injected into SCC7 tumor induced mice. Cell viability in response to drug loaded and unloaded micelles was studied in SCC7 cancer cells using the MTS assay. An in vivo tumor inhibition study was conducted by intravenous injection of paclitaxel-loaded HA micelle nanoparticles as well as control nanoparticles without paclitaxel. The shape of the nanomicelles was evaluated by loading them with hydrophobic superparamagnetic iron oxide nanoparticle and then visualizing them by TEM. In conclusion, paclitaxel-loaded HA nanoparticulate micelles might be found to be a specific and efficient chemotherapeutic treatment for CD44 overexpressing cancer cells.
authors.affiliation: Department of Radiology, Chonnam National University Hwasun Hospital, Chonnam National University Medical School, Gwangju 501-746, Korea. Electronic address: regeth@gmail.com.
authors.forename: Reju G
authors.initials: RG
authors.lastname: Thomas
authors.affiliation: DKC corporation (BioActs), Incheon, South Korea.
authors.forename: MyeongJu
authors.initials: M
authors.lastname: Moon
authors.affiliation: Department of Radiology, Chonnam National University Hwasun Hospital, Chonnam National University Medical School, Gwangju 501-746, Korea.
authors.forename: SeJy
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Radiology, Chonnam National University Hwasun Hospital, Chonnam National University Medical School, Gwangju 501-746, Korea. Electronic address: yjeong@jnu.ac.kr.
authors.forename: Yong Yeon
authors.initials: YY
authors.lastname: Jeong
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems; D006801:Humans; D018960:Hyaluronan Receptors / Q000187:drug effects; D006820:Hyaluronic Acid / Q000008:administration & dosage / Q000737:chemistry; D051379:Mice; D008823:Micelles; D058990:Molecular Targeted Therapy; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology; D017239:Paclitaxel / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: C497483:CD44 protein, human; D018960:Hyaluronan Receptors; D008823:Micelles; D006820:Hyaluronic Acid; D017239:Paclitaxel
keywords: Cancer; Hyaluronic acid; Nanoparticle