title: Evaluation of high-resolution melting curve analysis of ligation-mediated real-time PCR, a rapid method for epidemiological typing of ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter Species) pathogens.
abstract: A single-tube method, ligation-mediated real-time PCR high-resolution melt analysis (LMqPCR HRMA), was modified for the rapid typing of Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. (ESKAPE) pathogens. A 97% agreement (60/62 isolates) was achieved in comparison to pulsed-field gel electrophoresis (PFGE) results, which indicates that LMqPCR HRMA is a rapid and accurate screening tool for monitoring nosocomial outbreaks.
authors.affiliation: Department of Clinical Microbiology, Kalmar County Hospital, Kalmar, Sweden Department of Medicine and Optometry, Linnaeus University, Kalmar, Sweden hanna.woksepp@ltkalmar.se.
authors.forename: Hanna
authors.initials: H
authors.lastname: Woksepp
authors.affiliation: Department of Clinical Microbiology and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Ryberg
authors.affiliation: The Public Health Agency of Sweden, Solna, Sweden.
authors.forename: Hanna
authors.initials: H
authors.lastname: Billström
authors.affiliation: Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden.
authors.forename: Anita
authors.initials: A
authors.lastname: Hällgren
authors.affiliation: Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden.
authors.forename: Lennart E
authors.initials: LE
authors.lastname: Nilsson
authors.affiliation: Department of Chemistry and Biomedical Sciences, Linnaeus University, Kalmar, Sweden.
authors.forename: Britt-Inger
authors.initials: BI
authors.lastname: Marklund
authors.affiliation: The Public Health Agency of Sweden, Solna, Sweden.
authors.forename: Barbro
authors.initials: B
authors.lastname: Olsson-Liljequist
authors.affiliation: Department of Clinical Microbiology, Kalmar County Hospital, Kalmar, Sweden Department of Medicine and Optometry, Linnaeus University, Kalmar, Sweden Department of Clinical Microbiology and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
authors.forename: Thomas
authors.initials: T
authors.lastname: Schön
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology*; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016908:Gram-Positive Bacterial Infections / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors; D044366:Transition Temperature
chemical_list: D004269:DNA, Bacterial