title: Effect of chitosan concentration on PLGA microcapsules for controlled release and stability of resveratrol.
abstract: The polyphenols as nutraceutical and therapeutic agents are gaining growing interest for their beneficial effects and potential in human health. In order to protect their scaffolds and functionality, and to improve the bioavailability, the microencapsulation can represent a promising strategy. This study reports on the formulation of the natural resveratrol (RSV) into microcapsules (MCs) prepared by using different concentrations of chitosan (CS) and poly(D,L-lactic-co-glycolic acid) (PLGA) as polymeric matrix. MCs were prepared by W/O/W double emulsion method and characterized in terms of morphology, size, encapsulation efficiency, physicochemical and thermal properties. RSV release behavior from MCs was evaluated under simulated gastrointestinal fluids, and the long term stability was monitored at different storage conditions. MCs resulted to have spherical shape and different morphology, with size ranging from 11 to 20 μm, and encapsulation efficiencies of 40-52%, depending on the CS concentration. Moreover, MCs containing CS exhibited a significant lower release of RSV than those containing only PLGA. Furthermore, all tested formulations were able to ensure a good retention and stability of encapsulated RSV until 6 months. In summary, CS/PLGA MCs can be proposed as an attractive delivery system to control the release and long term protection of RSV.
authors.affiliation: Department of Chemistry and Pharmacy, University of Sassari, Via Vienna 2, 07100 Sassari, Italy; Laboratory of Nanomedicine, Department of Chemistry and Pharmacy, University of Sassari, c/o Porto Conte Ricerche, Tramariglio 07041 Alghero, Italy. Electronic address: vsanna@uniss.it.
authors.forename: Vanna
authors.initials: V
authors.lastname: Sanna
authors.affiliation: Porto Conte Ricerche, Località Tramariglio, 07041 Alghero, Italy.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Roggio
authors.affiliation: Department of Chemistry and Pharmacy, University of Sassari, Via Vienna 2, 07100 Sassari, Italy.
authors.forename: Nicolino
authors.initials: N
authors.lastname: Pala
authors.affiliation: Istituto di Scienze delle Produzioni Alimentari (ISPA), Consiglio Nazionale delle Ricerche (CNR), Sezione di Sassari, 07100 Sassari, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Marceddu
authors.affiliation: Department of Chemistry and Pharmacy, University of Sassari, Via Vienna 2, 07100 Sassari, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Lubinu
authors.affiliation: Department of Chemistry and Pharmacy, University of Sassari, Via Vienna 2, 07100 Sassari, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Mariani
authors.affiliation: Department of Chemistry and Pharmacy, University of Sassari, Via Vienna 2, 07100 Sassari, Italy; Laboratory of Nanomedicine, Department of Chemistry and Pharmacy, University of Sassari, c/o Porto Conte Ricerche, Tramariglio 07041 Alghero, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Sechi
mesh_terms: D002214:Capsules / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology*; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D002214:Capsules; D003692:Delayed-Action Preparations; D013267:Stilbenes; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D048271:Chitosan; D000077185:Resveratrol
keywords: Chitosan; Microcapsules; Poly(D,L-lactic-co-glycolic acid); Resveratrol