title: Stem cells from human exfoliated deciduous teeth differentiate into functional hepatocyte-like cells by herbal medicine.
abstract: Stem cells from human exfoliated deciduous teeth (SHEDs) are mesenchymal stem cells isolated from the exfoliated human deciduous incisor that can differentiate into a many cell types. In this study, we evaluated the effect of liquorice or angelica extracts on the hepatic differentiation potential of SHEDs cells. SHEDs cells cultured in medium containing liquorice extracts were analyzed for 1) changes in cellular morphology, 2) changes in hepatic gene expression, AFP (Alpha-fetoprotein) and ALB (Albumin), and 3) albumin secretion and urea synthesis activity. Our data show that the hepatic differentiation potential of SHEDs cells is enhanced by the presence of liquorice or angelica extracts in the culture medium. Our findings present new therapeutic possibilities for liver damage repair.
authors.affiliation: Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, 1 Sec. 3, Chung-Hsiao E. Rd, Taipei 10608, Taiwan.
authors.forename: Wen-Ta
authors.initials: WT
authors.lastname: Su
authors.affiliation: Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, 1 Sec. 3, Chung-Hsiao E. Rd, Taipei 10608, Taiwan.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Chen
mesh_terms: D029969:Angelica / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage; D006035:Glycyrrhiza / Q000737:chemistry*; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007180:Incisor / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage*; D014080:Tooth Exfoliation
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts
keywords: SHEDs; angelica; differentiation; hepatocytes; liquorice