title: Controlling laser-induced jet formation for bioprinting mesenchymal stem cells with high viability and high resolution.
abstract: Laser-assisted bioprinting is a versatile, non-contact, nozzle-free printing technique which has demonstrated high potential for cell printing with high resolution. Improving cell viability requires determining printing conditions which minimize shear stress for cells within the jet and cell impact at droplet landing. In this context, this study deals with laser-induced jet dynamics to determine conditions from which jets arise with minimum kinetic energies. The transition from a sub-threshold regime to jetting regime has been associated with a geometrical parameter (vertex angle) which can be harnessed to print mesenchymal stem cells with high viability using slow jet conditions. Finally, hydrodynamic jet stability is also studied for higher laser pulse energies which give rise to supersonic but turbulent jets.
authors.affiliation: INSERM U1026, Bioingénierie Tissulaire (BioTis) Université Bordeaux Segalen 146, rue Léo-Saignat Case 45 33076 BORDEAUX cedex-France.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ali
authors.forename: Emeline
authors.initials: E
authors.lastname: Pages
authors.forename: Alexandre
authors.initials: A
authors.lastname: Ducom
authors.forename: Aurelien
authors.initials: A
authors.lastname: Fontaine
authors.forename: Fabien
authors.initials: F
authors.lastname: Guillemot
mesh_terms: D000818:Animals; D062028:Bioprinting / Q000379:methods*; D001709:Biotechnology / Q000379:methods*; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology*; D057446:Hydrodynamics; D053844:Lasers, Solid-State; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice