title: Isolation and characterization of atrazine mineralizing Bacillus subtilis strain HB-6.
abstract: Atrazine is a widely used herbicide with great environmental concern due to its high potential to contaminate soil and waters. An atrazine-degrading bacterial strain HB-6 was isolated from industrial wastewater and the 16S rRNA gene sequencing identified HB-6 as a Bacillus subtilis. PCR assays indicated that HB-6 contained atrazine-degrading genes trzN, atzB and atzC. The strain HB-6 was capable of utilizing atrazine and cyanuric acid as a sole nitrogen source for growth and even cleaved the s-triazine ring and mineralized atrazine. The strain demonstrated a very high efficiency of atrazine biodegradation with a broad optimum pH and temperature ranges and could be enhanced by cooperating with other bacteria, suggesting its huge potential for remediation of atrazine-contaminated sites. To our knowledge, there are few Bacillus subtilis strains reported that can mineralize atrazine, therefore, the present work might provide some new insights on atrazine remediation.
authors.affiliation: College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agriculture University, People's Republic of China; Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, Nankai University, People's Republic of China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agriculture University, People's Republic of China.
authors.forename: Lusheng
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agriculture University, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agriculture University, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agriculture University, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
mesh_terms: D001280:Atrazine / Q000378:metabolism*; D001412:Bacillus subtilis / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D005798:Genes, Bacterial; D010802:Phylogeny; D014227:Triazines / Q000378:metabolism; D062065:Wastewater / Q000382:microbiology
chemical_list: D001280:Atrazine; D014227:Triazines; D062065:Wastewater; C004632:cyanuric acid