title: Dual specificity phosphatase 6 suppresses the growth and metastasis of prostate cancer cells.
abstract: Dual specificity phosphatase 6 (DUSP6) is expressed at low levels in numerous types of human cancer. The loss of DUSP6 plays a pivotal role in tumor progression; however, the role of DUSP6 in prostate cancer remains unclear. In this study, in vitro invasion assays and in vivo metastasis experiments were used to investigate the effects of DUSP6 on prostate cancer cell invasion and metastasis. Furthermore, in vitro growth and soft agar assays and in vivo growth experiments were performed to determine the function of DUSP6 in cell proliferation. The results showed that the overexpression of DUSP6 suppressed the invasion and growth of DU‑145 human prostate cancer cells, whereas knockdown of DUSP6 promoted the invasion and proliferation of LNCap human prostate adenocarcinoma cells. Further experiments demonstrated that the overexpression of DUSP6 inhibited the proliferation and liver metastasis of DU‑145 cells in mice. In addition, DUSP6 downregulated the expression of matrix metallopeptidase 3 and interleukin 8 in prostate cancer cells. Taken together, these findings indicate that DUSP6 may act as a negative mediator in the regulation of prostate cancer cell growth and metastasis.
authors.affiliation: Department of Urology, People's Hospital of Zhengzhou, Zhengzhou, Henan 450003, P.R. China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zhai
authors.affiliation: Department of Urology, People's Hospital of Zhengzhou, Zhengzhou, Henan 450003, P.R. China.
authors.forename: Qianhe
authors.initials: Q
authors.lastname: Han
authors.affiliation: Department of Urology, People's Hospital of Zhengzhou, Zhengzhou, Henan 450003, P.R. China.
authors.forename: Zhongjie
authors.initials: Z
authors.lastname: Shan
authors.affiliation: Department of Urology, People's Hospital of Zhengzhou, Zhengzhou, Henan 450003, P.R. China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Qu
authors.affiliation: Department of Urology, People's Hospital of Zhengzhou, Zhengzhou, Henan 450003, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Urology, People's Hospital of Zhengzhou, Zhengzhou, Henan 450003, P.R. China.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D054642:Dual Specificity Phosphatase 6 / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009362:Neoplasm Metastasis / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics*
chemical_list: D016209:Interleukin-8; C517035:DUSP6 protein, human; D054642:Dual Specificity Phosphatase 6; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3