title: M gene analysis of canine coronavirus strains detected in Korea.
abstract: The purpose of this study was to investigate the genetic features of canine coronavirus (CCV) strains detected in Korea. M gene sequences obtained for isolates from 22 dogs with enteritis over a 5-year period were evaluated. Sequence comparison revealed that the 22 Korean CCV strains had an 87.2 to 100% nucleotide homology. Comparing to the typical reference CCV strains (type II), the nucleotide sequence of Korean strains had homology ranged from 86.3% to 98.3% (89.1% to 99.2% for the amino acid sequence) and 87.7% to 97.8% (92.4% to 100% for the amino acid sequence) when compared to FCoV-like CCV strains (type I). Three amino acid variations in the M gene were characteristic for the Korean CCV strains. Phylogenetic analysis demonstrated that the 22 Korean CCV strains belonged to four typical CCV clusters (i.e., a unique Korean CCV cluster, a type II and transmissible gastroenteritis virus cluster, an intermediate cluster between type I and II, and a type I cluster). This study was the first to identify genetic differences of the M gene from Korean CCV strains and provided a platform for molecular identification of different Korean CCV strains.
authors.affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Seok-Young
authors.initials: SY
authors.lastname: Jeoung
authors.forename: So-Yun
authors.initials: SY
authors.lastname: Ann
authors.forename: Hyun-Tae
authors.initials: HT
authors.lastname: Kim
authors.forename: Doo
authors.initials: D
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018352:Coronavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017939:Coronavirus, Canine / Q000302:isolation & purification*; D004283:Dog Diseases / Q000453:epidemiology* / Q000821:virology; D004285:Dogs; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D056910:Republic of Korea / Q000453:epidemiology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D014763:Viral Matrix Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D014763:Viral Matrix Proteins
keywords: M gene; canine coronavirus; phylogenetic analysis