title: Sulfated hyaluronan influences the formation of artificial extracellular matrices and the adhesion of osteogenic cells.
abstract: The aim of this study is to compare differentially sulfated hyaluronan (sHA) derivatives and chondroitin sulfate (CS) with respect to their ability to influence the formation of artificial extracellular matrices (aECMs) during in vitro-fibrillogenesis of collagen type I at high- and low-ionic strength. Analysis is performed using turbidity, biochemical assays, atomic force (AFM), and transmission electron microscopy (TEM). In general, high-sulfated glycosaminoglycans (GAGs) associate to a higher amount with collagen than the low-sulfated ones. The addition of GAGs prior to fibrillogenesis at low-ionic strength results in a dose-dependent decrease in fibril diameter. At high-ionic strength these effects are only obtained for the sHA derivatives but not for CS. Likewise, increasing concentrations and degree of GAG sulfation strongly affected the kinetics of fibrillogenesis. The impact of sulfation degree on F-actin location and fiber formation in SaOS-2 cells implies that adhesion-related intracellular signaling is influenced to a variable extent.
authors.affiliation: Institute of Materials Science, Max Bergmann Center of Biomaterials, Technische Universität Dresden, 01069, Dresden, Germany.
authors.forename: Alina
authors.initials: A
authors.lastname: Miron
authors.forename: Sandra
authors.initials: S
authors.lastname: Rother
authors.forename: Linda
authors.initials: L
authors.lastname: Huebner
authors.forename: Ute
authors.initials: U
authors.lastname: Hempel
authors.forename: Iris
authors.initials: I
authors.lastname: Käppler
authors.forename: Stephanie
authors.initials: S
authors.lastname: Moeller
authors.forename: Matthias
authors.initials: M
authors.lastname: Schnabelrauch
authors.forename: Dieter
authors.initials: D
authors.lastname: Scharnweber
authors.forename: Vera
authors.initials: V
authors.lastname: Hintze
mesh_terms: D000199:Actins / Q000378:metabolism; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002809:Chondroitin Sulfates / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D010012:Osteogenesis; D015398:Signal Transduction
chemical_list: D000199:Actins; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates
keywords: atomic force microscopy (AFM); chondroitin sulfate; collagen fibrillogenesis; hyaluronan; matrix