title: Advanced glycation end products inhibitors from Alpinia zerumbet rhizomes.
abstract: Advanced glycation end products (AGEs) are major factors responsible for the complication of diabetes. The present study was carried out to investigate the inhibitory activities on fructosamine adduct and α-dicarbonyl formations by hexane extracts of various parts of Alpinia zerumbet. Furthermore, we isolated two previously known compounds, namely 5,6-dehydrokawain (DK) and dihydro-5,6-dehydrokawain (DDK). 8(17),12-Labdadiene-15,16-dial (labdadiene) was isolated for the first time from the rhizome of A. zerumbet. The results showed that labdadiene (IC50=51.06μg/mL) had similar activity to rutin and quercetin against fructosamine adduct. The inhibition of α-dicarbonyl compounds formation by labdadiene was significantly higher than that of DK and DDK. Our results indicate that labdadiene is a potent antiglycation agent which was found to inhibit AGEs formation in three different steps in the pathway. These data indicate that labdadiene could be used to prevent glycation-associated complications in diabetes.
authors.affiliation: Department of Bioscience and Biotechnology, The United Graduate School of Agricultural Science, Kagoshima University, Kagoshima 890-0065, Japan. Electronic address: mcmine56@yahoo.com.
authors.forename: Jamnian
authors.initials: J
authors.lastname: Chompoo
authors.affiliation: Department of Bioscience and Biotechnology, The United Graduate School of Agricultural Science, Kagoshima University, Kagoshima 890-0065, Japan. Electronic address: atul616@yahoo.com.
authors.forename: Atul
authors.initials: A
authors.lastname: Upadhyay
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Senbaru 1, Nishihara-cho, Okinawa 903-0219, Japan. Electronic address: K098963@eve.u-ryukyu.ac.jp.
authors.forename: Wataru
authors.initials: W
authors.lastname: Kishimoto
authors.affiliation: Life Up Clinic, 750 Minamiuehara, Nakagusuku, Okinawa 901-2424, Japan. Electronic address: dr@drmakise.com.
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Makise
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Senbaru 1, Nishihara-cho, Okinawa 903-0219, Japan. Electronic address: b986097@agri.u-ryukyu.ac.jp.
authors.forename: Shinkichi
authors.initials: S
authors.lastname: Tawata
keywords: 5,6-Dehydrokawain; 8(17),12-Labdadiene-15,16-dial; Advanced glycation end products (AGEs); Alpinia zerumbet; Diabetes; Dihydro-5,6-dehydrokawain