title: Human adipose tissue-derived mesenchymal stromal cells promote B-cell motility and chemoattraction.
abstract: OBJECTIVE
  Mesenchymal stromal cells hold special interest for cell-based therapy because of their tissue-regenerative and immunosuppressive abilities. B-cell involvement in chronic inflammatory and autoimmune pathologies makes them a desirable target for cell-based therapy. Mesenchymal stromal cells are able to regulate B-cell function; although the mechanisms are little known, they imply cell-to-cell contact.
  
  
  METHODS
  We studied the ability of human adipose tissue-derived mesenchymal stromal cells (ASCs) to attract B cells.
  
  
  RESULTS
  We show that ASCs promote B-cell migration through the secretion of chemotactic factors. Inflammatory/innate signals do not modify ASC capacity to mediate B-cell motility and chemotaxis. Analysis of a panel of B cell-related chemokines showed that none of them appeared to be responsible for B-cell motility. Other ASC-secreted factors able to promote cell motility and chemotaxis, such as the cytokine interleukin-8 and prostaglandin E2, did not appear to be implicated.
  
  
  CONCLUSIONS
  We propose that ASC promotion of B-cell migration by undefined secreted factors is crucial for ASC regulation of B-cell responses.
authors.affiliation: B Cell Dynamics Laboratory, Department of Immunology and Oncology, Centro Nacional de Biotecnología (CNB)-CSIC, UAM-Campus Cantoblanco, Madrid, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Barrio
authors.affiliation: B Cell Dynamics Laboratory, Department of Immunology and Oncology, Centro Nacional de Biotecnología (CNB)-CSIC, UAM-Campus Cantoblanco, Madrid, Spain.
authors.forename: Victor Delgado
authors.initials: VD
authors.lastname: Cuevas
authors.affiliation: TiGenix SAU, Parque Tecnológico de Madrid, Madrid, Spain.
authors.forename: Ramón
authors.initials: R
authors.lastname: Menta
authors.affiliation: TiGenix SAU, Parque Tecnológico de Madrid, Madrid, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Mancheño-Corvo
authors.affiliation: TiGenix SAU, Parque Tecnológico de Madrid, Madrid, Spain.
authors.forename: Olga
authors.initials: O
authors.lastname: delaRosa
authors.affiliation: TiGenix NV, Leuven, Belgium.
authors.forename: Wilfried
authors.initials: W
authors.lastname: Dalemans
authors.affiliation: TiGenix SAU, Parque Tecnológico de Madrid, Madrid, Spain.
authors.forename: Eleuterio
authors.initials: E
authors.lastname: Lombardo
authors.affiliation: B Cell Dynamics Laboratory, Department of Immunology and Oncology, Centro Nacional de Biotecnología (CNB)-CSIC, UAM-Campus Cantoblanco, Madrid, Spain. Electronic address: ycarrasco@cnb.csic.es.
authors.forename: Yolanda R
authors.initials: YR
authors.lastname: Carrasco
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D002633:Chemotaxis; D018920:Coculture Techniques; D015232:Dinoprostone / Q000378:metabolism*; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8; D015232:Dinoprostone
keywords: B cells; chemotaxis; mesenchymal stromal cells; migration