title: Streptomyces canchipurensis sp. nov., isolated from a limestone habitat.
abstract: Hundung Limestone habitat, Manipur, India is an unexplored site for microbial diversity studies. Using polyphasic taxonomy, a Streptomyces strain, MBRL 172(T), has been characterized. The strain was found to show highest 16S rRNA gene sequence similarity with Streptomyces coeruleofuscus NBRC 12757(T) (99.2 %). The DNA relatedness between MBRL 172(T) and S. coeruleofuscus NBRC 12757(T), and between MBRL 172(T) and Streptomyces nogalater NBRC 13445(T), were 36.8 ± 4.4 and 52.5 ± 2.7 %, respectively. Strain MBRL 172(T) was found to contain LL-diaminopimelic acid as the diagnostic diamino acid and glucose, mannose and xylose as the major sugars in whole cell hydrolysates. The polar lipids in the cell membrane were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositolmannoside. The predominant menaquinones detected were MK-9(H6) and MK-9(H8). The cellular fatty acids identified were mainly saturated fatty acids: anteiso-C15:0, iso-C16:0 and iso-C15:0. Based on differences in the biochemical and molecular characteristics from its closest relatives, the strain can be proposed to represent a novel taxon in the genus Streptomyces, for which the name Streptomyces canchipurensis is proposed, with the type strain MBRL 172(T) (=JCM 17575(T) = KCTC 29105(T)).
authors.affiliation: State Key Laboratory of Biocontrol, Key Laboratory of Biodiversity Dynamics and Conservation of Guangdong Higher Education Institutes College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China, liact@hotmail.com.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.forename: Salam
authors.initials: S
authors.lastname: Nimaichand
authors.forename: Zhao
authors.initials: Z
authors.lastname: Jiang
authors.forename: Min-Jiao
authors.initials: MJ
authors.lastname: Liu
authors.forename: Thi-Nhan
authors.initials: TN
authors.lastname: Khieu
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Kim
authors.forename: Wael N
authors.initials: WN
authors.lastname: Hozzein
authors.forename: Dong-Jin
authors.initials: DJ
authors.lastname: Park
authors.forename: Mohammed A M
authors.initials: MA
authors.lastname: Wadaan
authors.forename: Debananda S
authors.initials: DS
authors.lastname: Ningthoujam
mesh_terms: D015373:Bacterial Typing Techniques; D002119:Calcium Carbonate; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D004783:Environmental Microbiology; D005227:Fatty Acids / Q000032:analysis; D007194:India; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; D002119:Calcium Carbonate