title: Eye-mediated immune tolerance to Type II collagen in arthritis-prone strains of mice.
abstract: Type II collagen (CII) is a cartilage structural protein that plays important roles in joint function, arthritis and ageing. In studying the ability of CII to induce eye-mediated specific immune tolerance, we have recently proven that CII is capable of inducing anterior chamber-associated immune deviation (ACAID) in Balb/c mice. Here, we study the ability of CII to induce eye-mediated immune tolerance in strains of mice that are prone to the induction of rheumatoid arthritis. Thus, we hypothesized that CII induces ACAID in DBA/1 mice and in C57BL/6 mice through the AC route (direct injection) or the intravenous route (adoptive transfer of in vitro-generated CII-specific ACAID macrophages or of CII-specific in vitro-generated T regulatory cells). Specific immune tolerance induction was assessed using both delayed-type hypersensitivity (DTH) and local adoptive transfer (LAT) assays. Results indicated the ability of CII to generate CII-specific ACAID-mediated immune tolerance in vivo and in vitro in both DBA/1 mice and C57BL/6 mice. These findings could be beneficial in studies of immune tolerance induction using CII.
authors.affiliation: Department of Pharmacy Practice, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, USA.
authors.forename: Shukkur M
authors.initials: SM
authors.lastname: Farooq
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
authors.forename: Hossam M
authors.initials: HM
authors.lastname: Ashour
mesh_terms: D019264:Adoptive Transfer / Q000379:methods; D000818:Animals; D000867:Anterior Chamber / Q000187:drug effects / Q000276:immunology*; D001168:Arthritis / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D024043:Collagen Type II / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism; D005123:Eye / Q000187:drug effects / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology / Q000378:metabolism; D007108:Immune Tolerance / Q000276:immunology*; D007114:Immunization / Q000379:methods; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013154:Spleen / Q000166:cytology / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000637:transplantation
chemical_list: D024043:Collagen Type II
keywords: ACAID; C57BL/6 mice; DBA/1 mice; collagen type II; immune privilege; peripheral tolerance; regulatory T cells