title: Domibacillus enclensis sp. nov., isolated from marine sediment, and emended description of the genus Domibacillus.
abstract: A novel red-pigmented bacterial strain, designated NIO-1016(T), was isolated from a sediment sample from Chorao Island, India and was investigated by a polyphasic taxonomic approach. The strain was Gram-reaction-positive, strictly aerobic, motile and catalase-positive and produced spherical to slightly ellipsoidal endospores with subterminal position on swollen sporangia. The genomic DNA G+C content was 46.9 mol%. The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0 and C16 : 0. The predominant quinones were MK-6 (89.3 %) and MK-7 (8.7 %). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, and an unidentified phospholipid. meso-Diaminopimelic acid (type A1γ) was present in the cell-wall peptidoglycan and the major whole-cell sugars were glucose and ribose. The closest phylogenetic neighbours were identified as Domibacillus robiginosus DSM 25058(T) (98.0 % similarity) and Domibacillus indicus DSM 28032(T) (97.2 % similarity), other species of the genus Bacillus displayed below 96 % similarity. Phylogenetic, physiological, biochemical and morphological differences between strain NIO-1016(T) and its closest neighbours indicate that this strain represents a novel species in the genus Domibacillus in the family Bacillaceae for which the name Domibacillus enclensis sp. nov. is proposed with the type species NIO-1016(T) ( = DSM 25145(T) = NCIM 5462(T) = CCTCC AB 2011121(T)).
authors.affiliation: NCIM-Resource Center, CSIR-National Chemical Laboratory, Pune-411008, Maharashtra, India.
authors.forename: Vidya V
authors.initials: VV
authors.lastname: Sonalkar
authors.affiliation: NCIM-Resource Center, CSIR-National Chemical Laboratory, Pune-411008, Maharashtra, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Mawlankar
authors.affiliation: Microbial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sector-39A, Chandigarh-160036.
authors.forename: Srinivasan
authors.initials: S
authors.lastname: Krishnamurthi
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, PR China.
authors.forename: Shan-Kun
authors.initials: SK
authors.lastname: Tang
authors.affiliation: NCIM-Resource Center, CSIR-National Chemical Laboratory, Pune-411008, Maharashtra, India.
authors.forename: Syed G
authors.initials: SG
authors.lastname: Dastager
mesh_terms: D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D007194:India; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D010860:Pigments, Biological / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C042455:menaquinone 6; C062629:menaquinone 7