title: Discovery of a novel insulin-like peptide and insulin binding proteins in the Eastern rock lobster Sagmariasus verreauxi.
abstract: This study reports, for the first time in any of the commercially important decapod species, the identification of an insulin-like peptide (ILP), distinct from the androgenic gland hormone. Bioinformatics analysis of the de novo assembled spiny lobster, (Sagmariasus verreauxi) transcriptome, allowed identification of Sv-ILP1 as well as eight binding proteins. Binding proteins were termed as Sv-IGFBP, due to homology with the vertebrate insulin-like growth-factor binding protein and Sv-SIBD1-7, single insulin-binding domain protein (SIBD), similar to those identified in other invertebrate species. Sv-ILP1 was found to be expressed in the eyestalk, gonads and antennal gland of both sexes and to a lesser extent in male muscle, androgenic gland and hepatopancreas. The expression profiles of each binding protein were found to vary across tissues, with Sv-SIBD5, 6 and 7 showing higher expression in the gonad, demonstrated by PCR and digital gene expression. Further spatial investigations, using in-situ hybridisation, found Sv-ILP1 to be expressed in the neurosecretory cells of the thoracic ganglia, in keeping with the tissue expression of Drosophila ILP7 (DILP7). This correlative tissue expression, considered with the phylogenetic clustering of Sv-ILP1 and DILP7, suggests Sv-ILP1 to be a DILP7 orthologue. The broad expression of Sv-ILP1 strongly suggests that ILPs have a role beyond that of masculinisation in decapods. The function of these novel peptides may have application in enhancing aquaculture practices in the commercially important decapod species.
authors.affiliation: GeneCology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, 4 Locked Bag, Maroochydore, Queensland 4558, Australia.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Chandler
authors.affiliation: GeneCology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, 4 Locked Bag, Maroochydore, Queensland 4558, Australia.
authors.forename: Joseph
authors.initials: J
authors.lastname: Aizen
authors.affiliation: GeneCology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, 4 Locked Bag, Maroochydore, Queensland 4558, Australia.
authors.forename: Abigail
authors.initials: A
authors.lastname: Elizur
authors.affiliation: The Robert H. Smith Faculty of Agriculture, Food and Environment, Department of Animal Sciences, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
authors.forename: Lian
authors.initials: L
authors.lastname: Hollander-Cohen
authors.affiliation: Institute for Marine and Antarctic Studies, University of Tasmania, Private Bag 49, Hobart, Tasmania 7001, Australia.
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Battaglene
authors.affiliation: GeneCology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, 4 Locked Bag, Maroochydore, Queensland 4558, Australia. Electronic address: tventura@usc.edu.au.
authors.forename: Tomer
authors.initials: T
authors.lastname: Ventura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015415:Biomarkers / Q000378:metabolism*; D029721:Drosophila Proteins / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D017403:In Situ Hybridization; D007328:Insulin / Q000235:genetics / Q000378:metabolism*; D018969:Insulin-Like Growth Factor Binding Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D033521:Palinuridae / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D036361:Peptide Hormones / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D015415:Biomarkers; D029721:Drosophila Proteins; D007328:Insulin; D018969:Insulin-Like Growth Factor Binding Proteins; D009479:Neuropeptides; D036361:Peptide Hormones; D012333:RNA, Messenger; C527689:insulin-like peptide 7, Drosophila; C000602249:insulin-like peptide, Sagmariasus verreauxi
keywords: Crustacea; Drosophila ILP7 (DILP7); Insulin-like growth factor binding protein (IGFBP); Insulin-like peptide (ILP); Single insulin-binding domain (SIBD)