title: Microwave-assisted extraction of phycobiliproteins from Porphyridium purpureum.
abstract: In the present study, microwave-assisted extraction was first employed to extract the phycobiliproteins of Porphyridium purpureum (Pp). Freeze-dried Pp cells were subjected to microwave-assisted extraction (MAE) to extract phycoerythin (PE), phycocyanin (PC), and allophycocyanin (APC). MAE combined reproducibility and high extraction yields and allowed a 180- to 1,080-fold reduction of the extraction time compared to a conventional soaking process. The maximal PE extraction yield was obtained after 10-s MAE at 40 °C, and PE was thermally damaged at temperatures higher than 40 °C. In contrast, a flash irradiation for 10 s at 100 °C was the best process to efficiently extract PC and APC, as it combined a high temperature necessary to extract them from the thylakoid membrane to a short exposure to thermal denaturation. The extraction order of the three phycobiliproteins was coherent with the structure of Pp phycobilisomes. Moreover, the absorption and fluorescence properties of MAE extracted phycobiliproteins were stable for several months after the microwave treatment. Scanning electron microscopy indicated that MAE at 100 °C induced major changes in the Pp cell morphology, including fusion of the exopolysaccharidic cell walls and cytoplasmic membranes of adjacent cells. As a conclusion, MAE is a fast and high yield process efficient to extract and pre-purify phycobiliproteins, even from microalgae containing a thick exopolysaccharidic cell wall.
authors.affiliation: UMRi CNRS 7266 LIENSs University of La Rochelle, 17042, La Rochelle, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Juin
authors.forename: Jean-René
authors.initials: JR
authors.lastname: Chérouvrier
authors.forename: Valérie
authors.initials: V
authors.lastname: Thiéry
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Gagez
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Bérard
authors.forename: Nicolas
authors.initials: N
authors.lastname: Joguet
authors.forename: Raymond
authors.initials: R
authors.lastname: Kaas
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Cadoret
authors.forename: Laurent
authors.initials: L
authors.lastname: Picot
mesh_terms: D002851:Chromatography, High Pressure Liquid; D008872:Microwaves; D052979:Phycobiliproteins / Q000737:chemistry / Q000302:isolation & purification*; D010798:Phycocyanin / Q000737:chemistry / Q000302:isolation & purification*; D010799:Phycoerythrin / Q000737:chemistry / Q000302:isolation & purification*; D044665:Porphyridium / Q000737:chemistry
chemical_list: D052979:Phycobiliproteins; D010798:Phycocyanin; D010799:Phycoerythrin