title: Arabidopsis drought-induced protein Di19-3 participates in plant response to drought and high salinity stresses.
abstract: Di19 (drought-induced protein19) family is a novel type of Cys2/His2 zinc-finger proteins. In this study, Arabidopsis Di19-3 was functionally characterized. The experimental results revealed that AtDi19-3 is a transcriptional activator, and could bind to the TACA(A/G)T sequence. AtDi19-3 expression in plants was remarkably induced by NaCl, mannitol and abscisic acid (ABA). T-DNA insertion mutation of AtDi19-3 results in an increase in plant tolerance to drought and high salinity stresses and ABA, whereas overexpression of AtDi19-3 leads to a drought-, salt- and ABA-sensitive phenotype of the transgenic plants. In the presence of NaCl, mannitol or ABA, rates of seed germination and cotyledon greening in Atdi19-3 mutant were higher, but in AtDi19-3 overexpression transgenic plants were lower than those in wild type. Roots of Atdi19-3 mutant seedlings were longer, but those of AtDi19-3 overexpression transgenic seedlings were shorter than those of wild type. Chlorophyll and proline contents in Atdi19-3 mutant were higher, but in AtDi19-3 overexpression seedlings were lower than those in wild type. Atdi19-3 mutant showed greater drought-tolerance, whereas AtDi19-3 overexpression transgenic plants exhibited more drought-sensitivity than wild type. Furthermore, expression of the genes related to ABA signaling pathway was altered in Atdi19-3 mutant and AtDi19-3 transgenic plants. These data suggest that AtDi19-3 may participate in plant response to drought and salt stresses in an ABA-dependent manner.
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, 430079, China.
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Qin
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Deng-Di
authors.initials: DD
authors.lastname: Li
authors.forename: Wen-Liang
authors.initials: WL
authors.lastname: Xu
authors.forename: Yong
authors.initials: Y
authors.lastname: Zheng
authors.forename: Xue-Bao
authors.initials: XB
authors.lastname: Li
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D018525:Germination / Q000187:drug effects; D008353:Mannitol / Q000494:pharmacology; D016254:Mutagenesis, Insertional; D010641:Phenotype; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D011485:Protein Binding; D054712:Salinity; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D015533:Transcriptional Activation / Q000187:drug effects; D016335:Zinc Fingers
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; C508910:Di19 protein, Arabidopsis; D010937:Plant Growth Regulators; D008353:Mannitol; D012965:Sodium Chloride; D000040:Abscisic Acid