title: Effect of mild hypothermia on the expression of toll-like receptor 2 in lung tissues with experimental acute lung injury.
abstract: OBJECTIVE
  Our study aimed to determine the effect of mild hypothermia (MHT) on the expression of toll-like receptor 2 (TLR2) in lung tissue with acute lung injury. The animals were randomly divided into control, model and mild hypothermia groups.
  
  
  METHODS
  A total of 40 rats was used in the study. Acute lung injury was induced by lipopolysaccharide and MHT was maintained at 32.5∼33.0 °C using body surface ice-bag placement combined with animal thermostat system. The ratio of PaO2/FiO2 was recorded. The mRNA and protein expressions of TLR2 were measured by real-time polymerase chain reaction and western blotting respectively. Moreover, enzyme linked immunosorbent assay were used for the quantification of TNF-α.
  
  
  RESULTS
  The ratio of PaO2/FiO2 was increased by MHT. TLR2 and TNF-α were increased in the rat lung 1h and 8h in the rats with acute lung injury while they were significantly decreased by MHT. Histological examination revealed that MHT alleviated the degree of inflammation.
  
  
  CONCLUSIONS
  Our study suggested that MHT might improve the lung function by inhibiting the inflammation via down-regulating the expressions of TLR2 in the acute injury lung tissues.
authors.affiliation: Department of Intensive Care Unit, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Zhan-Hong
authors.initials: ZH
authors.lastname: Tang
authors.affiliation: Department of Intensive Care Unit, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Jun-Tao
authors.initials: JT
authors.lastname: Hu
authors.affiliation: Department of Intensive Care Unit, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Zhan-Chao
authors.initials: ZC
authors.lastname: Lu
authors.affiliation: Department of Intensive Care Unit, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Ji
authors.affiliation: Department of Intensive Care Unit, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Xian-Feng
authors.initials: XF
authors.lastname: Chen
authors.affiliation: Department of Intensive Care Unit, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Liang-Yan
authors.initials: LY
authors.lastname: Jiang
authors.affiliation: Department of Intensive Care Unit, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Intensive Care Unit, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Jia-Shu
authors.initials: JS
authors.lastname: Jiang
authors.affiliation: Department of Intensive Care Unit, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Pang
authors.affiliation: Department of Emergency Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China. Electronic address: lichaoqian@yahoo.com.cn.
authors.forename: Chao-Qian
authors.initials: CQ
authors.lastname: Li
mesh_terms: D055371:Acute Lung Injury / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D000818:Animals; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D007036:Hypothermia, Induced; D007249:Inflammation / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051381:Rats; D051195:Toll-Like Receptor 2 / Q000096:biosynthesis*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: C495343:Tlr2 protein, rat; D051195:Toll-Like Receptor 2; D014409:Tumor Necrosis Factor-alpha
keywords: Acute lung injury; Lipopolysaccharide; Mild hypothermia; TNF-α; Toll-like receptor 2