title: Protective effects of oral administration of yeast thioredoxin against gastric mucosal injury.
abstract: Thioredoxin (TRX) is a redox regulating protein which has protective effects against oxidative stress-induced damage to cells and tissues. In this study, we investigated the effects of orally administered TRX derived from edible yeast, Saccharomyces cerevisiae, on gastric mucosa. First, we examined the digestibility of orally administered yeast TRX in mice, and detected yeast TRX in the stomach for 4 h after administration. Next, we investigated the mitigation of gastric mucosal injury after the oral administration of yeast TRX in water-immersion restraint stress and HCl/ethanol-induced gastric ulcer models. Furthermore, we conducted DNA microarray analysis, using the HCl/ethanol-induced model, which revealed that several groups of genes related to tissue repair were upregulated in ulcer regions in the stomachs of rats administered with yeast TRX. These results demonstrated the viability of the use of oral administrations of yeast TRX to protect the gastric mucosa.
authors.affiliation: a Nagahama Institute for Biochemical Science, Oriental Yeast Co., Ltd. , Nagahama , Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Taketani
authors.forename: Kimihiro
authors.initials: K
authors.lastname: Kinugasa
authors.forename: Rie
authors.initials: R
authors.lastname: Kitajima
authors.forename: Shin
authors.initials: S
authors.lastname: Nishiumi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ashida
authors.forename: Hajime
authors.initials: H
authors.lastname: Nakamura
authors.forename: Tuyosi
authors.initials: T
authors.lastname: Fujita
authors.forename: Ken
authors.initials: K
authors.lastname: Kanzaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Masutani
authors.forename: Junji
authors.initials: J
authors.lastname: Yodoi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002460:Cell Line; D019610:Cytoprotection / Q000187:drug effects*; D004063:Digestion; D000431:Ethanol / Q000009:adverse effects; D005285:Fermentation; D005753:Gastric Mucosa / Q000187:drug effects* / Q000293:injuries*; D006851:Hydrochloric Acid / Q000009:adverse effects; D008297:Male; D051379:Mice; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D013276:Stomach Ulcer / Q000139:chemically induced / Q000517:prevention & control / Q000523:psychology; D013315:Stress, Psychological / Q000517:prevention & control; D013879:Thioredoxins / Q000008:administration & dosage* / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000431:Ethanol; D013879:Thioredoxins; D006851:Hydrochloric Acid
keywords: gastric mucosal injury; oral administration; thioredoxin; yeast