title: Uptake and metabolism of diclofenac in Typha latifolia--how plants cope with human pharmaceutical pollution.
abstract: The fate of pharmaceuticals in our environment is a very important issue for environmental and health research. Although these substances have been detected in environmental compartments in low concentration until now, they will pose considerable environmental risk to ecosystems, animals and human due to their biological activity. Alternative plant based removal technologies that make use of some potential wetland species like Phragmites or Typha within traditional wastewater treatment plants have to be established to cope with this "new generation" of pollutants. We investigated uptake and translocation of diclofenac (1mgl(-1)) in the macrophyte Typha latifolia L. during one week exposure in greenhouse experiments. Detoxification products and involved key enzymatic processes were identified. We also examined the oxidative stress induced by the treatment and the defense capacity of the plants. Rapid uptake and effective metabolism were observed, where glycoside and glutathione conjugates represent dominant metabolites. Up to seven-fold induction of glycosyltransferase activity was observed in roots, but not in shoots. Glutathione S-transferase activity was also induced, but to a lower extent. The activity changes of defense enzymes points to oxidative stress in the plants. Our results show that human pharmaceuticals can be metabolized by plants similar to xenobiotics, but that similarities to human metabolism are limited.
authors.affiliation: Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstraße 1, Neuherberg 85764, Germany. Electronic address: Bernadett.Bartha@lwf.bayern.de.
authors.forename: Bernadett
authors.initials: B
authors.lastname: Bartha
authors.affiliation: Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstraße 1, Neuherberg 85764, Germany. Electronic address: christian.huber@helmholtz-muenchen.de.
authors.forename: Christian
authors.initials: C
authors.lastname: Huber
authors.affiliation: Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstraße 1, Neuherberg 85764, Germany. Electronic address: peter.schroeder@helmholtz-muenchen.de.
authors.forename: Peter
authors.initials: P
authors.lastname: Schröder
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004008:Diclofenac / Q000378:metabolism* / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D006027:Glycosides / Q000378:metabolism; D016695:Glycosyltransferases / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D018517:Plant Roots / Q000378:metabolism*; D018520:Plant Shoots / Q000378:metabolism; D032401:Typhaceae / Q000187:drug effects / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism* / Q000494:pharmacology; D053833:Wetlands
chemical_list: D000975:Antioxidants; D006027:Glycosides; D014874:Water Pollutants, Chemical; D004008:Diclofenac; D016695:Glycosyltransferases; D005982:Glutathione Transferase; D005978:Glutathione
keywords: Diclofenac; LC–MS; Metabolism; Oxidative stress; Typha ssp.; Uptake