title: Transcriptional profiling of the lectin ArathEULS3 from Arabidopsis thaliana toward abiotic stresses.
abstract: The family of EUL-related lectins groups all proteins with an Euonymus lectin (EUL) domain, a protein motif which is highly conserved throughout the plant kingdom and occurs as part of many chimeric proteins with different domain architectures. The S3 type EUL lectin from Arabidopsis thaliana (ArathEULS3) has become the model protein within this EUL family. Based on sequence homology to an ABA/NaCl inducible gene from rice and some publicly available high-throughput micro-array data, it was hypothesized that ArathEULS3 is transcriptionally regulated by osmotic stress responses. Here we present a detailed expression analysis of the ArathEULS3 lectin gene. Under normal growth conditions, ArathEULS3 is stably expressed throughout plant development. After ABA, NaCl and methyl jasmonate (MeJA) treatments transcription is upregulated. Furthermore, in silico promoter and co-expression analyses suggested the A. thaliana Homeobox 7 (ATHB-7) as a candidate transcription factor that may regulate ArathEULS3 expression. Taken together, our data confirm that the ArathEULS3 lectin gene indeed shows a stress-inducible expression pattern. We speculate on a role for ArathEULS3 in the plant stress response.
authors.affiliation: Ghent University, Dept. Molecular Biotechnology, Lab of Biochemistry and Glycobiology, Coupure Links 653, 9000 Ghent, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Van Hove
authors.affiliation: Ghent University, Dept. Molecular Biotechnology, Lab of Biochemistry and Glycobiology, Coupure Links 653, 9000 Ghent, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Stefanowicz
authors.affiliation: Ghent University, Dept. Molecular Biotechnology, Lab of Biochemistry and Glycobiology, Coupure Links 653, 9000 Ghent, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: De Schutter
authors.affiliation: Ghent University, Dept. Molecular Biotechnology, Lab of Biochemistry and Glycobiology, Coupure Links 653, 9000 Ghent, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: Eggermont
authors.affiliation: Ghent University, Dept. Molecular Biotechnology, Lab of Biochemistry and Glycobiology, Coupure Links 653, 9000 Ghent, Belgium.
authors.forename: N
authors.initials: N
authors.lastname: Lannoo
authors.affiliation: Ghent University, Dept. Molecular Biotechnology, Lab of Biochemistry and Glycobiology, Coupure Links 653, 9000 Ghent, Belgium.
authors.forename: B
authors.initials: B
authors.lastname: Al Atalah
authors.affiliation: Ghent University, Dept. Molecular Biotechnology, Lab of Biochemistry and Glycobiology, Coupure Links 653, 9000 Ghent, Belgium. Electronic address: ElsJM.VanDamme@UGent.be.
authors.forename: E J M
authors.initials: EJ
authors.lastname: Van Damme
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D020869:Gene Expression Profiling; D017343:Genes, Plant; D030821:Plants, Genetically Modified; D013312:Stress, Physiological; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; D017931:DNA Primers
keywords: ABA; Arabidopsis; ArathEULS3; Expression; Lectin