title: eMethylsorb: electrochemical quantification of DNA methylation at CpG resolution using DNA-gold affinity interactions.
abstract: We report a simple electrochemical method referred to as "eMethylsorb" for the detection of DNA methylation. The method relies on the base dependent affinity interaction of DNA with gold. The methylation status of DNA is quantified by monitoring the electrochemical current as a function of the relative adsorption level of bisulphite treated DNA samples onto a bare gold electrode. This method can successfully distinguish methylated and unmethylated epigenotypes at single CpG resolution.
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD 4072, Australia. m.shiddiky@uq.edu.au lgcarrascosa@uq.edu.au.
authors.forename: Abu Ali Ibn
authors.initials: AA
authors.lastname: Sina
authors.forename: Sidney
authors.initials: S
authors.lastname: Howell
authors.forename: Laura G
authors.initials: LG
authors.lastname: Carrascosa
authors.forename: Sakandar
authors.initials: S
authors.lastname: Rauf
authors.forename: Muhammad J A
authors.initials: MJ
authors.lastname: Shiddiky
authors.forename: Matt
authors.initials: M
authors.lastname: Trau
mesh_terms: D018899:CpG Islands; D004247:DNA / Q000032:analysis*; D019175:DNA Methylation; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D013447:Sulfites / Q000737:chemistry
chemical_list: D013447:Sulfites; D006046:Gold; D004247:DNA; C042345:hydrogen sulfite