title: Molecular diversity of fungal and bacterial communities in the marine sponge Dragmacidon reticulatum.
abstract: The present work aimed to investigate the diversity of bacteria and filamentous fungi of southern Atlantic Ocean marine sponge Dragmacidon reticulatum using cultivation-independent approaches. Fungal ITS rDNA and 18S gene analyses (DGGE and direct sequencing approaches) showed the presence of representatives of three order (Polyporales, Malasseziales, and Agaricales) from the phylum Basidiomycota and seven orders belonging to the phylum Ascomycota (Arthoniales, Capnodiales, Dothideales, Eurotiales, Hypocreales, Pleosporales, and Saccharomycetales). On the other hand, bacterial 16S rDNA gene analyses by direct sequencing approach revealed the presence of representatives of seven bacterial phyla (Cyanobacteria, Proteobacteria, Actinobacteria, Bacteroidetes, Lentisphaerae, Chloroflexi, and Planctomycetes). Results from statistical analyses (rarefaction curves) suggested that the sampled clones covered the fungal diversity in the sponge samples studied, while for the bacterial community additional sampling would be necessary for saturation. This is the first report related to the molecular analyses of fungal and bacterial communities by cultivation-independent approaches in the marine sponges D. reticulatum. Additionally, the present work broadening the knowledge of microbial diversity associated to marine sponges and reports innovative data on the presence of some fungal genera in marine samples.
authors.affiliation: Divisão de Recursos Microbianos, Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas-CPQBA, Universidade Estadual de Campinas-UNICAMP, Rua Alexandre Cazelatto, Paulínia, SP, Brazil.
authors.forename: Michel R Z
authors.initials: MR
authors.lastname: Passarini
authors.forename: Paula B
authors.initials: PB
authors.lastname: Miqueletto
authors.forename: Valéria M
authors.initials: VM
authors.lastname: de Oliveira
authors.forename: Lara D
authors.initials: LD
authors.lastname: Sette
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D001203:Ascomycota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001267:Atlantic Ocean; D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D041963:Bacteroidetes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001487:Basidiomycota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D044822:Biodiversity; D041862:Chloroflexi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D000458:Cyanobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005658:Fungi / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D015723:Gene Library; D020459:Genes, rRNA; D010802:Phylogeny; D011161:Porifera / Q000382:microbiology*; D020560:Proteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: Cultivation-independent approach; DGGE; Direct sequencing; Marine sponge; Microbial diversity