title: Synthesis of propyl gallate by transesterification of tannic acid in aqueous media catalysed by immobilised derivatives of tannase from Lactobacillus plantarum.
abstract: Immobilised derivatives of tannase from Lactobacillus plantarum were able to catalyse the transesterification of tannic acid by using moderate concentrations of 1-propanol in aqueous media. Transesterification of tannic acid was very similar to transesterification of methyl gallate. The synthetic yield depended on the pH and concentration of 1-propanol, although it did not vary much when using 30% or 50% 1-propanol. Synthetic yields of 45% were obtained with 30% of 1-propanol at pH 5.0. The product was chromatographically pure, and the reaction by-product was 55% pure gallic acid. On the other hand, immobilised tannase was fairly stable under optimal reaction conditions.
authors.affiliation: Instituto de Fermentaciones Industriales, CSIC, Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: Gloria
authors.initials: G
authors.lastname: Fernandez-Lorente
authors.affiliation: Instituto de Catalisis, CSIC, Campus UAM-Cantoblanco, 28049 Madrid, Spain.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Bolivar
authors.affiliation: Instituto de Catalisis, CSIC, Campus UAM-Cantoblanco, 28049 Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Rocha-Martin
authors.affiliation: Instituto de Fermentaciones Industriales, CSIC, Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Curiel
authors.affiliation: Instituto de Fermentaciones Industriales, CSIC, Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: Rosario
authors.initials: R
authors.lastname: Muñoz
authors.affiliation: Instituto de Fermentaciones Industriales, CSIC, Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: Blanca
authors.initials: B
authors.lastname: de Las Rivas
authors.affiliation: Instituto de Fermentaciones Industriales, CSIC, Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: Alfonso V
authors.initials: AV
authors.lastname: Carrascosa
authors.affiliation: Instituto de Catalisis, CSIC, Campus UAM-Cantoblanco, 28049 Madrid, Spain. Electronic address: jmguisan@icp.csic.es.
authors.forename: Jose M
authors.initials: JM
authors.lastname: Guisan
keywords: 1-Propanol; Natural antioxidants; Transesterifications in aqueous media