title: Phosphoglycerate kinase enhanced immunity of the whole cell of Streptococcus agalactiae in tilapia, Oreochromis niloticus.
abstract: Streptococcus agalactiae is a Gram-positive bacterium and a severe aquaculture pathogen that can infect a wide range of warmwater fish species. The outer-surface proteins in bacterial pathogens play an important role in pathogenesis. We evaluated the immunogenicity of two of the identified surface proteins namely phosphoglycerate kinase (PGK) and ornithine carbamoyl-transferase (OCT). PGK and OCT were over-expressed and purified from Escherichia coli and used as the subunit vaccines in tilapia. Tilapia immunized with the S. agalactiae modified bacteria vaccine (whole cell preparations with recombinant PGK and OCT proteins) individually were tested for the efficacy. OCT and PGK combined with WC had a higher survival rate. A high-level protection and significant specific antibody responses against S. agalactiae challenge was observed upon the vaccinated tilapia with the purified PGK protein and S. agalactiae whole cells. The specific antibody titer against S. agalactiae antigen suggested that increased antibody titers were correlated with post-challenge survival rate. Il-1β expression profile was higher in PGK + WC-treated group. Tnf-α expression in the PGK + WC group was significantly increased. Taken together, our results suggested the combinations of recombinant protein and whole cell may elicit immune responses that reach greater protection than that of individual S. agalactiae components.
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan, ROC.
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Wang
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan, ROC.
authors.forename: Hsing-Yen
authors.initials: HY
authors.lastname: Huang
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan, ROC.
authors.forename: Ming-An
authors.initials: MA
authors.lastname: Tsai
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan, ROC.
authors.forename: Pei-Chi
authors.initials: PC
authors.lastname: Wang
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan, ROC.
authors.forename: Bo-Huang
authors.initials: BH
authors.lastname: Jiang
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan, ROC. Electronic address: scchen@mail.npust.edu.tw.
authors.forename: Shih-Chu
authors.initials: SC
authors.lastname: Chen
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology / Q000494:pharmacology*; D015153:Blotting, Western; D023681:Cichlids; D017931:DNA Primers / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology* / Q000517:prevention & control; D053583:Interleukin-1beta / Q000276:immunology; D009954:Ornithine Carbamoyltransferase / Q000276:immunology; D010735:Phosphoglycerate Kinase / Q000276:immunology*; D060888:Real-Time Polymerase Chain Reaction; D013290:Streptococcal Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D013292:Streptococcus agalactiae / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D017931:DNA Primers; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D009954:Ornithine Carbamoyltransferase; D010735:Phosphoglycerate Kinase
keywords: Ornithine carbamoyl-transferase; Phosphoglycerate kinase; Streptococcus agalactiae; Vaccine efficacy