title: β2 integrins (CD11/18) are essential for the chemosensory adhesion and migration of polymorphonuclear leukocytes on bacterial cellulose.
abstract: Bacterial cellulose (BC) has been studied widely for applications in biomedical materials such as prosthetic artificial blood vessels owing to its unique characteristics, which include nontoxicity and nonimmunogenicity as compared with synthetic biopolymers such as expanded polytetrafluorethylene (ePTFE). However, to date, studies on the relative effect of leukocytes on BC as a prosthetic vascular graft are insufficient. Polymorphonuclear leukocytes (PMN) play a pivotal role in early-phase immune response to bacterial or periprosthetic infection. PMN recruitment at sites of infection or inflammation mediated by various integrins such as β2 integrin family (CD11/CD18 family). Therefore, we discuss our investigations into the mechanisms by which β2 integrins-mediated chemosensory adhesion and migration of PMN on the vascular graft surface, BC. Our results show that CD11b/CD18 components mainly mediate PMN adherence on BC. CD11b/CD18 displays weak coordination with the other two α subunits (CD11a and CD11c). Furthermore, it was found that the β subunit (CD18) plays a critical role in both the adhesion and migration of N-formylmethionyl-leucyl-phenylalanine (fMLP)-stimulated PMN on BC. The activity of CD18 contrasts with that of the individual α subunits. Among these, only CD11b displayed inhibition of PMN migration on BC surfaces.
authors.affiliation: Department of Microbiology, School of Medicine, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
authors.forename: Gun-Dong
authors.initials: GD
authors.lastname: Kim
authors.forename: Seung Eun
authors.initials: SE
authors.lastname: Lee
authors.forename: Hana
authors.initials: H
authors.lastname: Yang
authors.forename: Hye Rim
authors.initials: HR
authors.lastname: Park
authors.forename: Gun Woo
authors.initials: GW
authors.lastname: Son
authors.forename: Cheung-Seog
authors.initials: CS
authors.lastname: Park
authors.forename: Yong Seek
authors.initials: YS
authors.lastname: Park
mesh_terms: D018821:CD18 Antigens / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D002465:Cell Movement / Q000187:drug effects*; D002482:Cellulose / Q000494:pharmacology*; D041822:Gluconacetobacter xylinus / Q000737:chemistry*; D006801:Humans; D008856:Microscopy, Fluorescence; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*
chemical_list: D018821:CD18 Antigens; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002482:Cellulose
keywords: Gluconacetobacter xylinus; N-formylmethionyl-leucyl-phenylalanine (fMLP); bacterial cellulose (BC); polymorphonuclear leukocytes (PMN); β2 integrins (CD11/CD18)