title: Nanochannel-based electrochemical assay for transglutaminase activity.
abstract: A novel electrochemical assay to quantify transglutaminase activity is reported. The assay is based on the enzyme-controlled diffusion of Fe(CN)6(3-/4-) through amino-functionalized nanochannels of a mesoporous silica thin film on a Au surface in the presence of N-benzyloxycarbonyl-L-glutaminylglycine.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040-Madrid, Spain. pingarro@quim.ucm.es rvillalonga@quim.ucm.es.
authors.forename: Iñigo
authors.initials: I
authors.lastname: Fernández
authors.forename: Alfredo
authors.initials: A
authors.lastname: Sánchez
authors.forename: Paula
authors.initials: P
authors.lastname: Díez
authors.forename: Paloma
authors.initials: P
authors.lastname: Martínez-Ruiz
authors.forename: Prospero
authors.initials: P
authors.lastname: Di Pierro
authors.forename: Raffaele
authors.initials: R
authors.lastname: Porta
authors.forename: Reynaldo
authors.initials: R
authors.lastname: Villalonga
authors.forename: José M
authors.initials: JM
authors.lastname: Pingarrón
mesh_terms: D004058:Diffusion; D055664:Electrochemical Techniques; D004789:Enzyme Activation; D057075:Enzyme Assays / Q000379:methods*; D005292:Ferricyanides / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry* / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties; D011503:Transglutaminases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005292:Ferricyanides; D006046:Gold; D012822:Silicon Dioxide; D011503:Transglutaminases