title: Antiviral activity of novel oseltamivir derivatives against some influenza virus strains.
abstract: The aim of this study was to investigate the in vitro cytotoxicity of oseltamivir derivatives and determine their activity against A/H1N1/PR/8/34 and A/H3N2/HongKong/8/68 - strains of influenza virus. Antiviral activity of these compounds was determined by using two methods. MTT staining was used to assess the viability of MDCK cells infected with influenza viruses and treated with various concentrations of drugs. In parallel, the effect of drugs on viral replication was assessed using the hemagglutination test. The most toxic compounds were: OS-64, OS-35, OS-29, OS-27 and OS-25, whereas OS-11, OS-20 and OS-23 were the least toxic ones. Statistically significant antiviral effect at a higher virus dose was shown by compounds: OS-11, OS-20, OS-27, OS-35, and OS-64. H3N2 virus was sensitive to 10-times lower concentrations of OS-11 and OS-35 than H1N1. At a lower infection dose, the antiviral activity was observed for OS-11, OS 27, OS-35 and OS-20. OS-64 turned out to be effective only at a high concentration. OS-23 showed no antiviral effect.
authors.affiliation: Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Kocik
authors.affiliation: Biological Threats Identification and Countermeasure Center of MIHE, Puławy, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Kołodziej
authors.affiliation: Biological Threats Identification and Countermeasure Center of MIHE, Puławy, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Joniec
authors.affiliation: Biological Threats Identification and Countermeasure Center of MIHE, Puławy, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kwiatek
authors.affiliation: Biological Threats Identification and Countermeasure Center of MIHE, Puławy, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Bartoszcze
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D004285:Dogs; D005021:Ethylamines / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects* / Q000502:physiology; D053122:Influenza A Virus, H3N2 Subtype / Q000187:drug effects* / Q000502:physiology; D007251:Influenza, Human / Q000188:drug therapy; D061985:Madin Darby Canine Kidney Cells; D053139:Oseltamivir / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D005021:Ethylamines; C510851:N-phenylethyl-N-ethyl-2-chloroethylamine; D053139:Oseltamivir