title: Object-location training elicits an overlapping but temporally distinct transcriptional profile from contextual fear conditioning.
abstract: Hippocampus-dependent learning is known to induce changes in gene expression, but information on gene expression differences between different learning paradigms that require the hippocampus is limited. The bulk of studies investigating RNA expression after learning use the contextual fear conditioning task, which couples a novel environment with a footshock. Although contextual fear conditioning has been useful in discovering gene targets, gene expression after spatial memory tasks has received less attention. In this study, we used the object-location memory task and studied gene expression at two time points after learning in a high-throughput manner using a microfluidic qPCR approach. We found that expression of the classic immediate-early genes changes after object-location training in a fashion similar to that observed after contextual fear conditioning. However, the temporal dynamics of gene expression are different between the two tasks, with object-location memory producing gene expression changes that last at least 2 hours. Our findings indicate that different training paradigms may give rise to distinct temporal dynamics of gene expression after learning.
authors.affiliation: Pharmacology Graduate Group, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Shane G
authors.initials: SG
authors.lastname: Poplawski
authors.affiliation: Cell and Molecular Biology Graduate Group, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Hannah
authors.initials: H
authors.lastname: Schoch
authors.affiliation: Department of Psychiatry, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Wimmer
authors.affiliation: Department of Cell Biology, Yale University, New Haven, CT 06520, USA.
authors.forename: Joshua D
authors.initials: JD
authors.lastname: Hawk
authors.affiliation: Departments of Psychology and Mathematics, Swarthmore College, Swarthmore, PA, 19081, United States.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Walsh
authors.affiliation: Centre for the Cellular Basis of Behaviour, King's College London, London, UK SE5 9NU.
authors.forename: Karl P
authors.initials: KP
authors.lastname: Giese
authors.affiliation: Pharmacology Graduate Group, University of Pennsylvania, Philadelphia, PA 19104, USA.|Cell and Molecular Biology Graduate Group, University of Pennsylvania, Philadelphia, PA 19104, USA.|Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Ted
authors.initials: T
authors.lastname: Abel
mesh_terms: D000818:Animals; D003214:Conditioning, Classical / Q000502:physiology*; D005239:Fear / Q000502:physiology*; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000378:metabolism*; D018782:Maze Learning / Q000502:physiology*; D008568:Memory / Q000502:physiology*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger
keywords: Fear conditioning; Hippocampus; Object-location memory; Spatial memory; Transcription