title: The involvement of neuronal nitric oxide synthase in antiepileptic action of alpha-asarone on pentylenetetrazol molding rats.
abstract: The aim of the present study was to research the role of nitric oxide (NO) as a mediator of alpha (α)-asarone effect at the pentylenetetrazol (PTZ)-induced epileptiform discharge in rat. α-Asarone that was injected intraperitoneally twenty minutes before PTZ injection suppressed the clonic discharge effectively and the significant actions lasted for 30 min with no change of clonic amplitude. Administration of α-asarone did not influence interictal discharge. Four kinds of NO regulators were administered, including non-selective NG-nitro-L-arginine methyl ester (L-NAME), selective neuronal nitric oxide synthase (nNOS) inhibitor, 7-nitroindazole (7-NI), inducible nitric oxide synthase (iNOS) inhibitor, aminoguanidine (AG) and NO substrate, L-arginine (ARG) and their influence on the actions of α-asarone were studied, and all of the regulators were administered fifteen minutes before α-asarone injection. L-NAME and 7-NI reversed the anticlonic activity of α-asarone, and a significant increase of clonic activity was induced by L-NAME later in L-NAME +.α-asarone + PTZ group. There were no significant differences between AG + α-asarone + PTZ and α-asarone + PTZ group. L-ARG played a dual role in this study. It aggravated clonic discharge in the early stage but relieved interictal discharge in the late stage compared with PTZ group alone, and the beneficial effect of α-asarone was also reversed. All the above results suggest that nNOS/NO pathway mediates the anticonvulsant effect of α-asarone, and NO played a biphasic role in PTZ modeling process, while iNOS was unrelated to the inhibition effect of α-asarone on PTZ induced epileptiform activity.
authors.affiliation: Department of Neurosurgery and Epilepsy Center, the Second Affiliated Hospital of Dalian Medical University, Dalian, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Su
authors.affiliation: Dalian Medical University, Dalian, China.
authors.forename: Wenting
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Regenerative Medicine Centre, the First Affiliated Hospital of Dalian Medical University, Dalian, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery and Epilepsy Center, the Second Affiliated Hospital of Dalian Medical University, Dalian, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of Neurosurgery and Epilepsy Center, the Second Affiliated Hospital of Dalian Medical University, Dalian, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qin
authors.affiliation: Department of Neurology, the First Affiliated Hospital of Dalian Medical University, Dalian, China.
authors.forename: Changbin
authors.initials: C
authors.lastname: Jiang
mesh_terms: D000200:Action Potentials / Q000187:drug effects*; D000081248:Allylbenzene Derivatives; D000818:Animals; D000840:Anisoles / Q000008:administration & dosage*; D001921:Brain / Q000187:drug effects / Q000503:physiopathology*; D003292:Convulsants; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004827:Epilepsy / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology*; D005260:Female; D009569:Nitric Oxide / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D010433:Pentylenetetrazole; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome
chemical_list: D000081248:Allylbenzene Derivatives; D000840:Anisoles; D003292:Convulsants; C012195:asarone; D009569:Nitric Oxide; D052248:Nitric Oxide Synthase Type I; D010433:Pentylenetetrazole
keywords: Alpha-asarone; epileptiform activity; nitric oxide synthase; pentylenetetrazol