title: Lrp13 is a novel vertebrate lipoprotein receptor that binds vitellogenins in teleost fishes.
abstract: Transcripts encoding a novel member of the lipoprotein receptor superfamily, termed LDL receptor-related protein (Lrp)13, were sequenced from striped bass (Morone saxatilis) and white perch (Morone americana) ovaries. Receptor proteins were purified from perch ovary membranes by protein-affinity chromatography employing an immobilized mixture of vitellogenins Aa and Ab. RT-PCR revealed lrp13 to be predominantly expressed in striped bass ovary, and in situ hybridization detected lrp13 transcripts in the ooplasm of early secondary growth oocytes. Quantitative RT-PCR confirmed peak lrp13 expression in the ovary during early secondary growth. Quantitative mass spectrometry revealed peak Lrp13 protein levels in striped bass ovary during late-vitellogenesis, and immunohistochemistry localized Lrp13 to the oolemma and zona radiata of vitellogenic oocytes. Previously unreported orthologs of lrp13 were identified in genome sequences of fishes, chicken (Gallus gallus), mouse (Mus musculus), and dog (Canis lupus familiaris). Zebrafish (Danio rerio) and Nile tilapia (Oreochromis niloticus) lrp13 loci are discrete and share genomic synteny. The Lrp13 appears to function as a vitellogenin receptor and may be an important mediator of yolk formation in fishes and other oviparous vertebrates. The presence of lrp13 orthologs in mammals suggests that this lipoprotein receptor is widely distributed among vertebrates, where it may generally play a role in lipoprotein metabolism.
authors.affiliation: Departments of Applied Ecology North Carolina State University, Raleigh, NC.
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Reading
authors.affiliation: Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan.
authors.forename: Naoshi
authors.initials: N
authors.lastname: Hiramatsu
authors.affiliation: Departments of Applied Ecology North Carolina State University, Raleigh, NC.
authors.forename: Justin
authors.initials: J
authors.lastname: Schilling
authors.affiliation: Departments of Applied Ecology North Carolina State University, Raleigh, NC.
authors.forename: Katelyn T
authors.initials: KT
authors.lastname: Molloy
authors.affiliation: Genomic Sciences Laboratory, North Carolina State University, Raleigh, NC.
authors.forename: Norm
authors.initials: N
authors.lastname: Glassbrook
authors.affiliation: Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Mizuta
authors.affiliation: Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan.
authors.forename: Wenshu
authors.initials: W
authors.lastname: Luo
authors.affiliation: Biological Sciences, North Carolina State University, Raleigh, NC.
authors.forename: David A
authors.initials: DA
authors.lastname: Baltzegar
authors.affiliation: Departments of Applied Ecology North Carolina State University, Raleigh, NC.
authors.forename: Valerie N
authors.initials: VN
authors.lastname: Williams
authors.affiliation: Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Todo
authors.affiliation: Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Hara
authors.affiliation: Biological Sciences, North Carolina State University, Raleigh, NC Carolina AquaGyn, Raleigh, NC.
authors.forename: Craig V
authors.initials: CV
authors.lastname: Sullivan
mesh_terms: D000818:Animals; D001492:Bass; D003001:Cloning, Molecular; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D018110:Receptors, Lipoprotein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014819:Vitellogenins / Q000378:metabolism*
chemical_list: D029941:Fish Proteins; D018110:Receptors, Lipoprotein; D014819:Vitellogenins
keywords: affinity purification; egg; endocytosis; low density lipoprotein receptor-related protein 13; oocyte; oogenesis; ovary; very low density lipoprotein receptor; vitellogenesis; yolk