title: Switching back to normal diet following high-fat diet feeding reduces cardiac vulnerability to ischaemia and reperfusion injury.
abstract: BACKGROUND
  We have recently shown that hearts of mice fed high-fat diet exhibit increased vulnerability to ischaemia and reperfusion (I/R) in parallel to changes in catalase protein expression, mitochondrial morphology and intracellular diastolic Ca(2+).
  
  
  OBJECTIVE
  To determine whether switching from high-fat back to normal diet alters vulnerability to I/R and to investigate cardiac cellular remodelling in relation to the mechanism(s) underlying I/R injury.
  
  
  RESULTS
  Male C57BL/6J mice were fed a high-fat diet for 19-22 weeks; after which a subset of mice was switched back to normal diet for 4-6 weeks. Hearts from mice switched back to normal diet were more resistant to reperfusion injury compared to hearts from mice fed only high-fat diet. This was associated with a significant reversal in catalase expression (western blotting) and recovery of size and density of mitochondria (electron microscopy). In contrast, switching back to normal diet did not alter cardiomyocyte contractility or Ca(2+) transients compared to high-fat diet.
  
  
  CONCLUSIONS
  This study shows for the first time that switching the diet from high-fat back to normal reduces vulnerability to I/R. This effect is associated with changes in catalase levels and mitochondrial morphology without altering cardiomyocyte contractility or Ca(2+) transients.
authors.affiliation: Bristol Heart Institute, School of Clinical Sciences, Faculty of Medicine and Dentistry, University of Bristol, Bristol, UK.
authors.forename: Ben
authors.initials: B
authors.lastname: Littlejohns
authors.forename: Hua
authors.initials: H
authors.lastname: Lin
authors.forename: Gianni D
authors.initials: GD
authors.lastname: Angelini
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Halestrap
authors.forename: M Saadeh
authors.initials: MS
authors.lastname: Suleiman
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D059305:Diet, High-Fat / Q000379:methods; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008929:Mitochondria, Heart / Q000378:metabolism / Q000502:physiology; D009200:Myocardial Contraction / Q000502:physiology; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000502:physiology*
chemical_list: D002374:Catalase; D002118:Calcium