title: Sprayed cells and polyelectrolyte films for biomaterial functionalization: the influence of physical PLL-PGA film treatments on dental pulp cell behavior.
abstract: Further development of biomaterials is expected as advanced therapeutic products must be compliant to good manufacturing practice regulations. A spraying method for building-up polyelectrolyte films followed by the deposition of dental pulp cells by spraying is presented. Physical treatments of UV irradiation and a drying/wetting process are applied to the system. Structural changes and elasticity modifications of the obtained coatings are revealed by atomic force microscopy and by Raman spectroscopy. This procedure results in thicker, rougher and stiffer film. The initially ordered structure composed of mainly α helices is transformed into random/β-structures. The treatment enhanced dental pulp cell adhesion and proliferation, suggesting that this system is promising for medical applications.
authors.affiliation: EA4203 Laboratoire de Bio-santé et Nano-science, Université Montpellier 1, Montpellier, France. ivan.panayotov@gmail.com.
authors.forename: Ivan V
authors.initials: IV
authors.lastname: Panayotov
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Collart-Dutilleul
authors.forename: Hamideh
authors.initials: H
authors.lastname: Salehi
authors.forename: Marta
authors.initials: M
authors.lastname: Martin
authors.forename: Attila
authors.initials: A
authors.lastname: Végh
authors.forename: Jacques
authors.initials: J
authors.lastname: Yachouh
authors.forename: Boyan
authors.initials: B
authors.lastname: Vladimirov
authors.forename: Péter
authors.initials: P
authors.lastname: Sipos
authors.forename: Balázs
authors.initials: B
authors.lastname: Szalontai
authors.forename: Csilla
authors.initials: C
authors.lastname: Gergely
authors.forename: Frédéric J G
authors.initials: FJ
authors.lastname: Cuisinier
mesh_terms: D000293:Adolescent; D001672:Biocompatible Materials / Q000737:chemistry*; D002470:Cell Survival; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D011099:Polyglutamic Acid / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry*; D014466:Ultraviolet Rays; D017155:Wettability
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011107:Polylysine; D011099:Polyglutamic Acid
keywords: biomaterials; cell spray deposition; dental pulp cells; polyelectrolyte multilayers