title: Preparative separation and purification of gingerols from ginger (Zingiber officinale Roscoe) by high-speed counter-current chromatography.
abstract: A novel method for purifying gingerols from ginger was developed using a high-speed counter-current chromatography (HSCCC). The two-phase solvent system such as light petroleum (bp 60-90°C)-ethyl acetate-methanol-water (5:5:6.5:3.5, v/v/v/v) was applied to the separation and purification of 6-, 8- and 10-gingerol from a crude extract of ginger. The experiment yielded 30.2mg of 6-gingerol, 40.5mg of 8-gingerol, 50.5mg of 10-gingerol from 200mg of crude extract in one-step separation. And the purity of these compounds was 99.9%, 99.9% and 99.2%, respectively, as determined by high-performance liquid chromatography (HPLC). Their structures were identified by gas chromatography-mass spectrometry (GC/MS) and (1)H, (13)C nuclear magnetic resonance (NMR).
authors.affiliation: Office of Academic Affairs, Shandong Agricultural University, Tai'an 271018, China; State Key Laboratory of Crop Biology, Tai'an 271018, Shandong, China.
authors.forename: Kunyou
authors.initials: K
authors.lastname: Zhan
authors.affiliation: State Key Laboratory of Crop Biology, Tai'an 271018, Shandong, China; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, Shandong, China. Electronic address: xukun@sdau.edu.cn.
authors.forename: Kun
authors.initials: K
authors.lastname: Xu
authors.affiliation: College of Chemistry and Material Science, Shandong Agricultural University, Tai'an 271018, China. Electronic address: hzyin@sdau.edu.cn.
authors.forename: Hongzong
authors.initials: H
authors.lastname: Yin
keywords: 10-Gingerol; 6-Gingerol; 8-Gingerol; High speed counter-current chromatography; Isolation