title: Protective effect of resveratrol on fluoride induced alteration in protein and nucleic acid metabolism, DNA damage and biogenic amines in rat brain.
abstract: Fluoride, a well-established environmental carcinogen, has been found to cause various neurodegenerative diseases in human. Sub-acute exposure to fluoride at a dose of 20mg/kgb.w./day for 30 days caused significant alteration in pro-oxidant/anti-oxidant status of brain tissue as reflected by perturbation of reduced glutathione content, increased lipid peroxidation, protein carbonylation, nitric oxide and free hydroxyl radical production and decreased activities of antioxidant enzymes. Decreased proteolytic and transaminase enzymes' activities, protein and nucleic acid contents and associated DNA damage were observed in the brain of fluoride intoxicated rats. The neurotransmitters dopamine (DA), norepinephrine (NE) and serotonin level was also significantly altered after fluoride exposure. Protective effect of resveratrol on fluoride-induced metabolic and oxidative dysfunctions was evaluated. Resveratrol was found to inhibit changes in metabolic activities restoring antioxidant status, biogenic amine level and structural organization of the brain. Our findings indicated that resveratrol imparted antioxidative role in ameliorating fluoride-induced metabolic and oxidative stress in different regions of the brain.
authors.affiliation: Nutritional Biochemistry Laboratory, Department of Human Physiology, Tripura University (A Central University), Suryamaninagar, West Tripura, Agartala 799022, India. Electronic address: sudiptapal@tripurauniv.in.
authors.forename: Sudipta
authors.initials: S
authors.lastname: Pal
authors.affiliation: Nutritional Biochemistry Laboratory, Department of Human Physiology, Tripura University (A Central University), Suryamaninagar, West Tripura, Agartala 799022, India.
authors.forename: Chaitali
authors.initials: C
authors.lastname: Sarkar
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000494:pharmacology; D001679:Biogenic Amines / Q000378:metabolism*; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects; D005459:Fluorides / Q000633:toxicity; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008297:Male; D018696:Neuroprotective Agents / Q000008:administration & dosage* / Q000494:pharmacology; D009696:Nucleic Acids / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D001679:Biogenic Amines; D018696:Neuroprotective Agents; D009696:Nucleic Acids; D013267:Stilbenes; D000077185:Resveratrol; D005459:Fluorides
keywords: Biogenic amines; Fluoride; Nucleic acids; Oxidative stress; Proteolytic activity; Resveratrol