title: Three-dimensional analysis of alveolar wall destruction in the early stage of pulmonary emphysema.
abstract: The morphological mechanism of alveolar wall destruction during pulmonary emphysema has not been clarified. The aim of this study was to elucidate this process three-dimensionally. Lung specimens from five patients with pulmonary emphysema were used, and five controls with normal alveolar structure were also examined. Sections 150 μm thick were stained with hematoxylin and eosin, elastica, and silver impregnation, and immunostained with selected antibodies. We examined these sections three-dimensionally using a laser confocal microscope and a light microscope. There were only a few Kohn's pores and no fenestrae in the normal alveoli from the controls. In the lungs of the emphysema patients a small rupture appeared in the extremely thin alveolar wall among the alveolar capillaries. This rupture enlarged to form a circle surrounded by the capillaries, which was called an alveolar fenestra. Two neighboring fenestrae fused by breakdown of the collapsed or cord-like capillary between them to form a large fenestra. The large fenestrae fused repeatedly to become larger, and these were bordered by thick elastic fibers constructing an alveolar framework. Alveolar wall destruction during emphysema could start from small ruptures of the alveolar wall that become fenestrae surrounded by capillaries, which fuse repeatedly to become larger fenestrae rimmed with elastic fibers. The alveolar capillary network could initially prevent enlargement of the fenestrae, and the thick elastic fibers constituting the alveolar framework could secondarily prevent destruction of the alveolar wall structure.
authors.affiliation: Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Uehara
authors.forename: Kenji
authors.initials: K
authors.lastname: Kawasaki
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Sugano
authors.forename: Takehisa
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Gou
authors.initials: G
authors.lastname: Matsumoto
authors.forename: Takayuki
authors.initials: T
authors.lastname: Honda
mesh_terms: D000368:Aged; D004396:Coloring Agents; D005260:Female; D006801:Humans; D021621:Imaging, Three-Dimensional; D008297:Male; D018613:Microscopy, Confocal; D011650:Pulmonary Alveoli / Q000473:pathology*; D011656:Pulmonary Emphysema / Q000473:pathology*; D013194:Staining and Labeling
chemical_list: D004396:Coloring Agents
keywords: alveolar capillary; alveolar wall; chronic obstructive pulmonary disease; elastic fiber; emphysema