title: Enhanced fermentative capacity of yeasts engineered in storage carbohydrate metabolism.
abstract: During yeast biomass production, cells are grown through several batch and fed-batch cultures on molasses. This industrial process produces several types of stresses along the process, including thermic, osmotic, starvation, and oxidative stress. It has been shown that Saccharomyces cerevisiae strains with enhanced stress resistance present enhanced fermentative capacity of yeast biomass produced. On the other hand, storage carbohydrates have been related to several types of stress resistance in S. cerevisiae. Here we have engineered industrial strains in storage carbohydrate metabolism by overexpressing the GSY2 gene, that encodes the glycogen synthase enzyme, and deleting NTH1 gene, that encodes the neutral trehalase enzyme. Industrial biomass production process simulations were performed with control and modified strains to measure cellular carbohydrates and fermentation capacity of the produced biomass. These modifications increased glycogen and trehalose levels respectively during bench-top trials of industrial biomass propagation. We finally show that these strains display an improved fermentative capacity than its parental strain after biomass production. Modification of storage carbohydrate content increases fermentation or metabolic capacity of yeast which can be an interesting application for the food industry.
authors.affiliation: Dept. de Biotecnología, Inst. de Agroquímica y Tecnología de Alimentos, IATA-CSIC, Valencia, Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: Pérez-Torrado
authors.forename: Emilia
authors.initials: E
authors.lastname: Matallana
mesh_terms: D018533:Biomass; D050260:Carbohydrate Metabolism / Q000235:genetics* / Q000502:physiology*; D005285:Fermentation; D006003:Glycogen / Q000032:analysis / Q000378:metabolism; D007218:Industrial Microbiology; D060847:Metabolic Engineering / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D014199:Trehalose / Q000032:analysis / Q000378:metabolism
chemical_list: D006003:Glycogen; D014199:Trehalose
keywords: S. cerevisiae; fermentative capacity; storage carbohydrates