title: A fluorescent probe for imaging p53-MDM2 protein-protein interaction.
abstract: In this article, we describe a no-wash small-molecule fluorescent probe for detecting and imaging p53-MDM2 protein-protein interaction based on an environment-sensitive fluorescent turn-on mechanism. After extensive biological examination, this probe L1 exhibited practical activity and selectivity in vitro and in cellulo.
authors.affiliation: Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Shandong University, Jinan, Shandong, 250012, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Zhenyuan
authors.initials: Z
authors.lastname: Miao
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Kun
authors.initials: K
authors.lastname: Fang
authors.forename: Chunlin
authors.initials: C
authors.lastname: Zhuang
authors.forename: Lupei
authors.initials: L
authors.lastname: Du
authors.forename: Chunquan
authors.initials: C
authors.lastname: Sheng
authors.forename: Minyong
authors.initials: M
authors.lastname: Li
mesh_terms: D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D061848:Optical Imaging / Q000379:methods*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D060066:Protein Interaction Maps; D051736:Proto-Oncogene Proteins c-mdm2 / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D016159:Tumor Suppressor Protein p53; D051736:Proto-Oncogene Proteins c-mdm2
keywords: MDM2; cell imaging; p53; protein-protein interaction; small-molecule fluorescent probe