title: Inhibitory effect of aqueous extracts from Miracle Fruit leaves on mutation and oxidative damage.
abstract: This study investigated the inhibitory effects of aqueous extracts from Miracle Fruit leaves (AML) on mutation and oxidative damage. The results showed that AML in the range of 1-5mg/plate inhibited the mutagenicity of 2-aminoanthracene (2-AA), an indirect mutagen, and 4-nitroquinoline-N-oxide (4-NQO), a direct mutagen toward Salmonella typhimurium TA 98 and TA 100. On the other hand, AML in the range of 0.05-0.2mg/ml showed radical scavenging, reducing activities, liposome protection as well as decreased tert-butyl hydroperoxide (t-BHP) induced oxidative cytotoxicity in HepG2 cells. High performance liquid chromatography (HPLC) analysis suggested that the active phenolic constituents in AML are p-hydroxybenzoic acid, vanillic acid, syringic acid, trans-p-coumaric acid and veratric acid. These active phenolic components may contribute to the biological protection effects of AML in different models. The data suggest that AML exhibiting biological activities can be applied to antimutation as well as anti-oxidative damage.
authors.affiliation: Department of Food Science, National Taiwan Ocean University, No. 2, Pei-Ning Road, Keelung, Taiwan, ROC.
authors.forename: Tai-Yuan
authors.initials: TY
authors.lastname: Chen
authors.affiliation: Department of Health & Nutrition, Chia-Nan University of Pharmacy and Science, No. 60, Erh-Jen Rd., Sec. 1, Tainan, Taiwan, ROC.
authors.forename: Zhi-Chyang
authors.initials: ZC
authors.lastname: Kang
authors.affiliation: Department of Applied Life Science & Health, Chia-Nan University of Pharmacy and Science, No. 60, Erh-Jen Rd., Sec. 1, Tainan, Taiwan, ROC.
authors.forename: Ming-Tsung
authors.initials: MT
authors.lastname: Yen
authors.affiliation: Department of Cosmetic Science, Chia-Nan University of Pharmacy and Science, No. 60, Erh-Jen Rd., Sec. 1, Tainan, Taiwan, ROC.
authors.forename: Ming-Hsing
authors.initials: MH
authors.lastname: Huang
authors.affiliation: Department of Applied Life Science & Health, Chia-Nan University of Pharmacy and Science, No. 60, Erh-Jen Rd., Sec. 1, Tainan, Taiwan, ROC. Electronic address: wangbs@mail.chna.edu.tw.
authors.forename: Bor-Sen
authors.initials: BS
authors.lastname: Wang
mesh_terms: D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D012486:Salmonella typhimurium / Q000187:drug effects; D032133:Synsepalum / Q000737:chemistry
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D005978:Glutathione
keywords: HPLC; Miracle Fruit leaves; Mutation; Oxidation