title: Sucrose enhances the accumulation of anthocyanins and glucosinolates in broccoli sprouts.
abstract: The germination rate, fresh weight, as well as the contents of anthocyanins and glucosinolates in broccoli sprouts treated with different kinds of sugars, including sucrose, glucose, fructose, mannitol, and fructose/glucose (1:1), were investigated. The results showed that all the sugars induced the accumulation of anthocyanins and glucosinolates with sucrose being the most effective one. In accordance with the accumulation of anthocyanins and glucosinolates, the antioxidant level increased while treated with sucrose. The effect of sucrose on expression of genes related to anthocyanin and glucosinolate biosynthesis were also investigated. The genes involved in the biosynthesis and transcriptional regulation of anthocyanin as well as glucosinolate biosynthetic gene Bo-Elong were all up-regulated by sucrose within 12h after the sucrose treatment. The application of sucrose improved the nutrition value of broccoli sprouts by enhancing the biosynthesis of anthocyanin and glucosinolate at the level of transcription.
authors.affiliation: Department of Horticulture, Zhejiang University, Hangzhou 310058, China.
authors.forename: Rongfang
authors.initials: R
authors.lastname: Guo
authors.affiliation: Department of Horticulture, Zhejiang University, Hangzhou 310058, China.
authors.forename: Gaofeng
authors.initials: G
authors.lastname: Yuan
authors.affiliation: Department of Horticulture, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou 310029, China. Electronic address: qmwang@zju.edu.cn.
authors.forename: Qiaomei
authors.initials: Q
authors.lastname: Wang
keywords: Anthocyanins; Brassica oleracea var. italica; Glucosinolates; Sprouts; Sucrose