title: Platelet-rich plasma-containing fragmin-protamine micro-nanoparticles promote epithelialization and angiogenesis in split-thickness skin graft donor sites.
abstract: BACKGROUND
  Platelet-rich plasma (PRP) contains multiple growth factors, and fragmin-protamine micro-nanoparticles (F-P M-NPs) significantly enhance and stabilize growth factors. The purpose of this study was to evaluate the effects of PRP-containing F-P M-NPs (PRP&F-P M-NPs) on wound repair in split-thickness skin graft (STSG-) donor sites (DS).
  
  
  METHODS
  A total of 56 inbred male rats were anesthetized and split-thickness skin graft donor site (STSG-DS) were created with a Padgett dermatome. PRP&F-P M-NPs, F-P M-NPs, PRP, and saline (control) were then intradermally injected evenly into the STSG-DSs. On 3, 4, 5, 7, and 10 d after creation of STSG-DS, skin sample sections were stained with hematoxylin and eosin to evaluate reepithelialization and angiogenesis.
  
  
  RESULTS
  Treatment of STSG-DS with PRP&F-P M-NPs effectively promoted epithelialization and new vessel formation compared with those treated with PRP, F-P M-NPs, and control (saline).
  
  
  CONCLUSIONS
  The intradermal injection of PRP&F-P M-NPs promotes epithelialization and angiogenesis in STSG-DS wounds.
authors.affiliation: Department of Plastic and Reconstructive Surgery, National Defense Medical College, Tokorozawa, Saitama, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takabayashi
authors.affiliation: Division of Biomedical Engineering, Research Institute, National Defense Medical College, Tokorozawa, Saitama, Japan. Electronic address: ishihara@ndmc.ac.jp.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishihara
authors.affiliation: Department of Plastic and Reconstructive Surgery, National Defense Medical College, Tokorozawa, Saitama, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Sumi
authors.affiliation: Department of Medical Engineering, National Defense Medical College, Tokorozawa, Saitama, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Takikawa
authors.affiliation: Division of Biomedical Engineering, Research Institute, National Defense Medical College, Tokorozawa, Saitama, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Nakamura
authors.affiliation: Department of Plastic and Reconstructive Surgery, National Defense Medical College, Tokorozawa, Saitama, Japan.
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Kiyosawa
mesh_terms: D000818:Animals; D017985:Dalteparin / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects*; D007271:Injections, Intradermal; D008297:Male; D053758:Nanoparticles; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D053657:Platelet-Rich Plasma; D011479:Protamines / Q000494:pharmacology*; D011916:Rats, Inbred F344; D012038:Regeneration; D012867:Skin / Q000098:blood supply / Q000166:cytology / Q000293:injuries; D016038:Skin Transplantation / Q000379:methods*; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects
chemical_list: D011479:Protamines; D017985:Dalteparin
keywords: Angiogenesis; Epithelialization; Fragmin-protamine micro-nanoparticles; Platelet-rich plasma; Split-thickness skin graft donor sites