title: A new microcolumn-type microchip for examining the expression of chimeric fusion genes using a nucleic acid sandwich hybridization technique.
abstract: We report a new type of microcolumn installed in a microchip. The architecture allows use of a nucleic acid sandwich hybridization technique to detect a messenger RNA (mRNA) chain as a target. Data are presented that demonstrate that the expression of a chimeric fusion gene can be detected. The microcolumn was filled with semi-transparent microbeads made of agarose gel that acted as carriers, allowing increased efficiency of the optical detection of fluorescence from the microcolumn. The hybrid between the target trapped on the microbeads and a probe DNA labeled with a fluorescent dye was detected by measuring the intensity of the fluorescence from the microcolumn directly. These results demonstrate an easy and simple method for determining the expression of chimeric fusion genes with no preamplification.
authors.affiliation: R&D Div., Medical Business Unit, Sony Corporation, 5-1-12 Kitashinagawa, Shinagawa-ku, Tokyo 141-0001 Japan. Electronic address: Michihiro.Ohnishi@jp.sony.com.
authors.forename: Michihiro
authors.initials: M
authors.lastname: Ohnishi
authors.affiliation: R&D Div., Medical Business Unit, Sony Corporation, 5-1-12 Kitashinagawa, Shinagawa-ku, Tokyo 141-0001 Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Sasaki
authors.affiliation: R&D Div., Medical Business Unit, Sony Corporation, 5-1-12 Kitashinagawa, Shinagawa-ku, Tokyo 141-0001 Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Kishimoto
authors.affiliation: R&D Div., Medical Business Unit, Sony Corporation, 5-1-12 Kitashinagawa, Shinagawa-ku, Tokyo 141-0001 Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Watanabe
authors.affiliation: Pediatrics and Developmental Biology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Takagi
authors.affiliation: Pediatrics and Developmental Biology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
authors.forename: Shuki
authors.initials: S
authors.lastname: Mizutani
authors.affiliation: R&D Div., Medical Business Unit, Sony Corporation, 5-1-12 Kitashinagawa, Shinagawa-ku, Tokyo 141-0001 Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Kishii
authors.affiliation: R&D Div., Medical Business Unit, Sony Corporation, 5-1-12 Kitashinagawa, Shinagawa-ku, Tokyo 141-0001 Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Yasuda
mesh_terms: D001483:Base Sequence; D002845:Chromatography / Q000295:instrumentation*; D015342:DNA Probes; D004867:Equipment Design; D005456:Fluorescent Dyes; D056656:Lab-On-A-Chip Devices; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins
keywords: Chimeric fusion gene; Fluorescent detection; Gene expression; Microcolumn chromatography; Nucleic acid sandwich hybridization; Semi-transparent microbead