title: Quantitative assessment of the multivalent protein-carbohydrate interactions on silicon.
abstract: A key challenge in the development of glycan arrays is that the sensing interface be fabricated reliably so as to ensure the sensitive and accurate analysis of the protein-carbohydrate interaction of interest, reproducibly. These goals are complicated in the case of glycan arrays as surface sugar density can influence dramatically the strength and mode of interaction of the sugar ligand at any interface with lectin partners. In this Article, we describe the preparation of carboxydecyl-terminated crystalline silicon (111) surfaces onto which are grafted either mannosyl moieties or a mixture of mannose and spacer alcohol molecules to provide "diluted" surfaces. The fabrication of the silicon surfaces was achieved efficiently through a strategy implicating a "click" coupling step. The interactions of these newly fabricated glycan interfaces with the lectin, Lens culinaris, have been characterized using quantitative infrared (IR) spectroscopy in the attenuated total geometry (ATR). The density of mannose probes and lectin targets was precisely determined for the first time by the aid of special IR calibration experiments, thus allowing for the interpretation of the distribution of mannose and its multivalent binding with lectins. These experimental findings were accounted for by numerical simulations of lectin adsorption.
authors.affiliation: Physique de la Matière Condensée, Ecole Polytechnique-CNRS , 91128 Palaiseau, France.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Jean-Noël
authors.initials: JN
authors.lastname: Chazalviel
authors.forename: Aloysius
authors.initials: A
authors.lastname: Siriwardena
authors.forename: Rabah
authors.initials: R
authors.lastname: Boukherroub
authors.forename: François
authors.initials: F
authors.lastname: Ozanam
authors.forename: Sabine
authors.initials: S
authors.lastname: Szunerits
authors.forename: Anne Chantal
authors.initials: AC
authors.lastname: Gouget-Laemmel
mesh_terms: D002241:Carbohydrates / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D015394:Molecular Structure; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012825:Silicon / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D002241:Carbohydrates; D011506:Proteins; D012825:Silicon