title: Genotoxic evaluation of terbinafine in human lymphocytes in vitro.
abstract: Terbinafine is an antimycotic drug usually used against several superficial fungal infections and with a potential application in the treatment of human cancers. Since to date there are few data on the genotoxic effects of terbinafine in mammalian cells, current study evaluated the potential genotoxic of such antifungal agent in cultured human peripheral blood lymphocytes. Terbinafine was used at the peak plasma concentration (1.0 μg/ml) and in four additional concentrations higher than the human plasmatic peak (5.0 μg/ml, 25.0 μg/ml, 50.0 μg/ml and 100.0 μg/ml). Chromosomal aberrations (CA), sister chromatid exchanges (SCE), micronuclei (MN), nucleoplasmic bridges (NP) and nuclear buds (NB) were scored as genetic endpoints. In all analysis no significant differences (α = 0.05, Kruskal-Wallis test) were observed. Complementary criterion adopted to obtain the final response in cytogenetic agreed with statistical results. Therefore, results of this study showed that terbinafine neither induced CA, SCE, MN, NP and NB nor affected significantly mitotic, replication and cytokinesis-block proliferation indices in any of the tested concentrations. It may be assumed that terbinafine was not genotoxic or cytotoxic to cultured human peripheral blood lymphocytes in our experimental conditions.
authors.affiliation: Departamento de Biotecnologia, Genética e Biologia Celular and.
authors.forename: Danielle
authors.initials: D
authors.lastname: Tolomeotti
authors.forename: Marialba Avezum Alves
authors.initials: MA
authors.lastname: de Castro-Prado
authors.forename: Juliane Rocha
authors.initials: JR
authors.lastname: de Sant'Anna
authors.forename: Ana Beatriz Tozzo
authors.initials: AB
authors.lastname: Martins
authors.forename: Valter Augusto
authors.initials: VA
authors.lastname: Della-Rosa
mesh_terms: D000328:Adult; D000935:Antifungal Agents / Q000633:toxicity*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D048749:Cytokinesis / Q000187:drug effects; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000473:pathology; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced; D015162:Micronucleus Tests; D008938:Mitosis / Q000187:drug effects; D009281:Naphthalenes / Q000633:toxicity*; D018570:Risk Assessment; D012854:Sister Chromatid Exchange / Q000187:drug effects; D000077291:Terbinafine; D055815:Young Adult
chemical_list: D000935:Antifungal Agents; D009281:Naphthalenes; D000077291:Terbinafine
keywords: Antifungal agent; chromosomal aberrations; micronuclei assay; sister chromatid exchanges