title: The effect of myoglobin crowding on the dynamics of water: an infrared study.
abstract: Solutions containing 8 and 32 wt% myoglobin are studied by means of infrared spectroscopy, as a function of temperature (290 K and lower temperatures), in the mid- and far-infrared spectral range. Moreover, ultrafast time-resolved infrared measurements are performed at ambient temperature in the O-D stretching region. The results evidence that the vibrational properties of water remain the same in these myoglobin solutions (anharmonicity, vibrational relaxation lifetime…) and in neat water. However, the collective properties of the water molecules are significantly affected by the presence of the protein: the orientational time increases, the solid-liquid transition is affected in the most concentrated solution and the dynamical transition of the protein is observed, from the point of view of water, even in the least concentrated solution, proving that the water and myoglobin dynamics are coupled.
authors.affiliation: Institut Rayonnement Matière de Saclay, LIDyL et Service Interdisciplinaire sur les Systèmes Moléculaires et les Matériaux, UMR 3299, CNRS/CEA, Groupe Physico-Chimie sous Rayonnement, Bâtiment 546, F-91191 Gif-sur-Yvette Cedex, France. sophie.le-caer@cea.fr.
authors.forename: S
authors.initials: S
authors.lastname: Le Caër
authors.forename: G
authors.initials: G
authors.lastname: Klein
authors.forename: D
authors.initials: D
authors.lastname: Ortiz
authors.forename: M
authors.initials: M
authors.lastname: Lima
authors.forename: S
authors.initials: S
authors.lastname: Devineau
authors.forename: S
authors.initials: S
authors.lastname: Pin
authors.forename: J-B
authors.initials: JB
authors.lastname: Brubach
authors.forename: P
authors.initials: P
authors.lastname: Roy
authors.forename: S
authors.initials: S
authors.lastname: Pommeret
authors.forename: W
authors.initials: W
authors.lastname: Leibl
authors.forename: R
authors.initials: R
authors.lastname: Righini
authors.forename: J P
authors.initials: JP
authors.lastname: Renault
mesh_terms: D009211:Myoglobin / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014867:Water / Q000737:chemistry*
chemical_list: D009211:Myoglobin; D014867:Water