title: Developmental changes in storage proteins and peptidyl prolyl cis-trans isomerase activity in grains of different wheat cultivars.
abstract: In the present study, storage proteins from five different wheat cultivars were extracted, fractionated and evaluated for their accumulation at different stages of development. SDS-PAGE analysis revealed that the accumulation of high molecular weight glutenin subunits was cultivar and stage dependent. However, low molecular weight glutenin subunits' accumulation was not altered significantly after 16days post anthesis in any of the cultivars. The rheological parameters (storage- and loss-modulus) of dough and gluten showed close association with either gliadins or glutenins. Peptidyl prolyl cis-trans isomerase (PPIase) activity, measured at different stages of grains development, showed variability with both the developmental stage and cultivar, and appeared to be primarily due to cyclophilins. Principal component analysis revealed the association of PPIase activity with either gliadin or total proteins, suggesting their significant role in the deposition of storage proteins in wheat.
authors.affiliation: Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab 143005, India.
authors.forename: Tanima
authors.initials: T
authors.lastname: Dutta
authors.affiliation: Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab 143005, India.
authors.forename: Harsimran
authors.initials: H
authors.lastname: Kaur
authors.affiliation: Department of Food Science and Technology, Guru Nanak Dev University, Amritsar, Punjab 143005, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Singh
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Akanksha
authors.initials: A
authors.lastname: Mishra
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Jayant K
authors.initials: JK
authors.lastname: Tripathi
authors.affiliation: Department of Food Science and Technology, Guru Nanak Dev University, Amritsar, Punjab 143005, India.
authors.forename: Narpinder
authors.initials: N
authors.lastname: Singh
authors.affiliation: Stress Physiology and Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Ashwani
authors.initials: A
authors.lastname: Pareek
authors.affiliation: Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab 143005, India. Electronic address: prabhjeet_s@yahoo.com.
authors.forename: Prabhjeet
authors.initials: P
authors.lastname: Singh
keywords: Gliadins; Glutenins; Peptidyl-prolyl cis-trans isomerase; Proteins; Rheology; Wheat