title: Protective effects of Scutellaria lindbergii root extract against oxidative-induced cell and DNA damage in mouse fibroblast-like cells.
abstract: BACKGROUND
  Scutellaria lindbergii Rech. f. (Lamiaceae) is an Iranian species of Scutellaria which has been shown to exert antimicrobial, antioxidant and cytotoxic effects.
  
  
  OBJECTIVE
  The protective properties of total methanol extract (TME) of S. lindbergii and its fractions (defatted and CH2Cl2) were investigated against cytotoxic and genotoxic effects of H2O2 in NIH 3T3 cell line as non-malignant cells.
  
  
  METHODS
  The cells were incubated with different concentrations of S. lindbergii root extracts [TME (15-250 μg ml(-)(1)), defatted fraction (15-500 μg ml(-)(1)) and CH2Cl2 fraction (5-40 μg ml(-)(1))] and toxic concentration of H2O2 (200 µM) at 37 °C for 2 h concurrently and Cell viability was quantitated by MTT assay. The antigenotoxic effect of extracts was investigated using comet assay. The cells were incubated with extracts [TME (25-250 μg ml(-)(1)), defatted fraction (25-500 μg ml(-)(1)) and CH2Cl2 fraction (5-40 μg ml(-)(1))] and H2O2 (25 µM) at 4 °C for 20 min, then the comet assay was performed. DNA damage was expressed as percentage tail DNA.
  
  
  RESULTS
  Total methanol extract of S. lindbergii and its fractions had a significant inhibitory effect on DNA damage. The IC50 values of TME, defatted fraction and CH2Cl2 fraction against DNA damage were determined as 48, 138 and 8 μg ml(-)(1), respectively.
  
  
  CONCLUSIONS
  S. lindbergii extracts can prevent oxidative DNA damage, which is likely due to its flavonoids and phenolic compounds as antioxidant constituents.
authors.affiliation: Biotechnology Research Center, Mashhad University of Medical Sciences , Mashhad , Iran .
authors.forename: Melika
authors.initials: M
authors.lastname: Ehtesham-Gharaee
authors.forename: Ameneh
authors.initials: A
authors.lastname: Eshaghi
authors.forename: Susan
authors.initials: S
authors.lastname: Shojaee
authors.forename: Javad
authors.initials: J
authors.lastname: Asili
authors.forename: Seyed Ahmad
authors.initials: SA
authors.lastname: Emami
authors.forename: Javad
authors.initials: J
authors.lastname: Behravan
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mosaffa
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D019610:Cytoprotection; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006861:Hydrogen Peroxide / Q000633:toxicity*; D051379:Mice; D041681:NIH 3T3 Cells; D016877:Oxidants / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots; D010946:Plants, Medicinal; D031363:Scutellaria / Q000737:chemistry
chemical_list: D000975:Antioxidants; D016877:Oxidants; D010936:Plant Extracts; D006861:Hydrogen Peroxide
keywords: Anticytotoxicity; Lamiaceae; MTT assay; antigenotoxicity; comet assay; hydrogen peroxide