title: Sequence variation in two mitochondrial DNA regions and internal transcribed spacer among isolates of the nematode Oesophagostomum asperum originating from goats in Hunan Province, China.
abstract: The present study examined sequence variability in two mitochondrial DNA (mtDNA) regions, namely cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1), and internal transcribed spacer (ITS) of nuclear ribosomal DNA (rDNA) among Oesophagostomum asperum isolates from goats in Hunan Province, China. A portion of the cox1 (pcox1), nad1 (pnad1) genes and the ITS (ITS1+5.8S rDNA+ITS2) rDNA were amplified by polymerase chain reaction (PCR) separately from adult O. asperum individuals and the representative amplicons were subjected to sequencing from both directions. The lengths of pcox1, pnad1 and ITS rDNA were 366 bp, 681 bp and 785 bp, respectively. The A+T contents of gene sequences were 71.5-72% for pcox1, 73.7-74.2% for pnad1 and 58-58.8% for ITS rDNA. Intra-specific sequence variations within O. asperum were 0-1.6% for pcox1, 0-1.9% for pnad1 and 0-1.7% for ITS rDNA, while inter-specific sequence differences among members of the genus Oesophagostomum were significantly higher, being 11.1-12.5%, 13.3-17.7% and 8.5-18.6% for pcox1, pnad1 and ITS rDNA, respectively. Phylogenetic analyses using combined sequences of pcox1 and pnad1, with three different computational algorithms (Bayesian inference, maximum likelihood and maximum parsimony), revealed distinct groups with high statistical support. These findings demonstrated the existence of intra-specific variation in mtDNA and rDNA sequences among O. asperum isolates from goats in Hunan Province, China, and have implications for studying molecular epidemiology and population genetics of O. asperum.
authors.affiliation: College of Veterinary Medicine, Hunan Agricultural University,Changsha,Hunan Province,410128,PR China.
authors.forename: F
authors.initials: F
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Hunan Agricultural University,Changsha,Hunan Province,410128,PR China.
authors.forename: T
authors.initials: T
authors.lastname: Hu
authors.affiliation: College of Veterinary Medicine, Hunan Agricultural University,Changsha,Hunan Province,410128,PR China.
authors.forename: N C
authors.initials: NC
authors.lastname: Duan
authors.affiliation: Xiang Xi National Vocational Technical College,Jishou,Hunan Province,416000,PR China.
authors.forename: W Y
authors.initials: WY
authors.lastname: Li
authors.affiliation: Xiang Xi National Vocational Technical College,Jishou,Hunan Province,416000,PR China.
authors.forename: Q
authors.initials: Q
authors.lastname: Teng
authors.affiliation: Xiang Xi National Vocational Technical College,Jishou,Hunan Province,416000,PR China.
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Hunan Agricultural University,Changsha,Hunan Province,410128,PR China.
authors.forename: W
authors.initials: W
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Hunan Agricultural University,Changsha,Hunan Province,410128,PR China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Hunan Agricultural University,Changsha,Hunan Province,410128,PR China.
authors.forename: T Y
authors.initials: TY
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D018509:DNA, Helminth / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D015511:Goat Diseases / Q000469:parasitology*; D006041:Goats; D008297:Male; D008969:Molecular Sequence Data; D009814:Oesophagostomiasis / Q000469:parasitology / Q000662:veterinary*; D009815:Oesophagostomum / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny
chemical_list: D018509:DNA, Helminth; D004272:DNA, Mitochondrial