title: Relationship between expression of Livin and the biological behavior of human oral squamous cell carcinoma.
abstract: Livin is one of the most important members of the inhibitor protein (IAP) family. It is overexpressed in several types of tumors and may have prognostic significance. The purpose of this study was to investigate Livin expression in human oral squamous cell carcinomas (OSCCs) and to determine whether Livin affects tumor cell behavior in OSCC cell lines and thus evaluate its potential usefulness in serving as a possible target for molecular-targeted therapy in a preclinical model. The expression of Livin protein was investigated in human OSCC tissues through immunohistochemistry and western blot analysis. To evaluate the impact of Livin knockdown on the behavior of human OSCC cell lines, invasion, migration, proliferation and apoptosis assays using small-interfering RNA (siRNA) were performed. RT-PCR and western blot analysis were used to assess alteration of Livin expression at the mRNA and protein levels. The results revealed that expression of Livin was increased in the human OSCC tissues compared with the adjacent normal mucosa. In addition, immunoreactivity of Livin was expressed in 8 OSCC tissues (44.4%). Knockdown of Livin resulted in significantly reduced cell invasion, migration and proliferation in the human OSCC cells. Livin knockdown induced cell apoptosis in the human OSCC cells. Moreover, Livin inhibited apoptosis by suppressing the activity of caspases in the human OSCC cells. In conclusion, livin is associated with invasive and oncogenic phenotypes such as tumor cell invasion, tumor cell migration, tumor cell proliferation, and resistance to apoptosis in human OSCC cells.
authors.affiliation: Department of Otorhinolaryngology - Head and Neck Surgery, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun 519-809, Republic of Korea.
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Lee
authors.affiliation: Department of Otorhinolaryngology - Head and Neck Surgery, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun 519-809, Republic of Korea.
authors.forename: Tae Mi
authors.initials: TM
authors.lastname: Yoon
authors.affiliation: Department of Otorhinolaryngology - Head and Neck Surgery, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun 519-809, Republic of Korea.
authors.forename: Sun-Ae
authors.initials: SA
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun 519-809, Republic of Korea.
authors.forename: Young-Lan
authors.initials: YL
authors.lastname: Park
authors.affiliation: Department of Pathology, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun 519-809, Republic of Korea.
authors.forename: Kyung-Hwa
authors.initials: KH
authors.lastname: Lee
authors.affiliation: Department of Otorhinolaryngology - Head and Neck Surgery, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun 519-809, Republic of Korea.
authors.forename: Sang Chul
authors.initials: SC
authors.lastname: Lim
authors.affiliation: Department of Otorhinolaryngology - Head and Neck Surgery, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun 519-809, Republic of Korea.
authors.forename: Joon Kyoo
authors.initials: JK
authors.lastname: Lee
authors.affiliation: Department of Internal Medicine, Chonnam National University Medical School, Research Institute of Medical Sciences, Chonnam National University and Hwasun Hospital, Hwasun 519-809, Republic of Korea.
authors.forename: Young Eun
authors.initials: YE
authors.lastname: Joο
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000328:Adult; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism*; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000401:mortality; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C419866:BIRC7 protein, human; D051034:Inhibitor of Apoptosis Proteins; D009363:Neoplasm Proteins