title: Biological stability and activity of siRNA in ionic liquids.
abstract: We report substantially enhanced biological stability of siRNA in a hydrated ionic liquid (IL) based on buffered choline dihydrogen phosphate (CDP). The results show that hydrated CDP IL substantially prolongs the shelf-life of siRNA for up to three months in the presence of RNase A. Remarkably, siRNA stored in the IL remained active and eGFP siRNA exhibited clear gene knockdown effects in eGFP-expressing HeLa cells.
authors.affiliation: Department of Chemical Engineering, Monash University, Clayton 3800, VIC, Australia. wenlong.cheng@monash.edu.
authors.forename: Romiza R
authors.initials: RR
authors.lastname: Mazid
authors.forename: Upulie
authors.initials: U
authors.lastname: Divisekera
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Yang
authors.forename: Vijayaraghavan
authors.initials: V
authors.lastname: Ranganathan
authors.forename: Douglas R
authors.initials: DR
authors.lastname: MacFarlane
authors.forename: Christina
authors.initials: C
authors.lastname: Cortez-Jugo
authors.forename: Wenlong
authors.initials: W
authors.lastname: Cheng
mesh_terms: D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D052578:Ionic Liquids / Q000737:chemistry*; D017493:Leukocyte Common Antigens / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000378:metabolism; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D013816:Thermodynamics; D044366:Transition Temperature
chemical_list: D052578:Ionic Liquids; D034741:RNA, Small Interfering; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D012259:Ribonuclease, Pancreatic; D017493:Leukocyte Common Antigens