title: Netrin-1 with stem cells promote angiogenesis in limb ischemic rats.
abstract: BACKGROUND
  Recent findings have elucidated that netrin-1 has ability of promoting angiogenesis besides the functions in nervous system. Autologous mesenchymal stem cells (MSCs) transplantation is now proved to be an effective method to treat peripheral arterial disease. However there are still many patients who cannot complete full treatments. Therefore it is necessary to improve the effectiveness. This study estimated the curative effects in chronic limb ischemia when MSCs allied with netrin-1.
  
  
  METHODS
  Thirty-six rats were made into chronic limb ischemia models. They were randomly assigned to four groups, netrin-1 + MSCs group (treated with netrin-1 and MSCs derived from peripheral blood), MSCs group (treated with MSCs individually), netrin-1 group (treated with netrin-1 individually), and control group (treated with saline). Measurements of murine behaviors, vascular endothelial growth factor expression, and capillary density in ischemia limb were performed on days 7, 14, and 28 after treatments; measurements of contraction force in ischemia limb was performed on day 28 after treatments to compare differences among the groups.
  
  
  RESULTS
  Netrin-1 allied with MSCs significantly increased Tarlov score, vascular endothelial growth factor expression, capillary density, and muscular strength in ischemia limb.
  
  
  CONCLUSIONS
  Netrin-1 allied with MSCs derived from peripheral blood significantly promoted angiogenesis in aged rats with chronic limb ischemia. It may be a promising method of treating peripheral arterial disease in the future.
authors.affiliation: Department of Vascular Surgery, Zhongnan Hospital of Wuhan University, Zhongnan Hospital, Wuhan city, Hubei province, China; Department of Vascular Surgery, Affiliated Xiangyang Central Hospital of Hubei University of Arts and Science, Xiangyang Center Hospital, Xiangyang city, Hubei province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Vascular Surgery, Affiliated Xiangyang Central Hospital of Hubei University of Arts and Science, Xiangyang Center Hospital, Xiangyang city, Hubei province, China.
authors.forename: Dejie
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, Affiliated Xiangyang Central Hospital of Hubei University of Arts and Science, Xiangyang Center Hospital, Xiangyang city, Hubei province, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Vascular Surgery, Zhongnan Hospital of Wuhan University, Zhongnan Hospital, Wuhan city, Hubei province, China. Electronic address: tanz728@126.com.
authors.forename: Zui
authors.initials: Z
authors.lastname: Tan
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D001162:Arteriosclerosis Obliterans / Q000473:pathology / Q000628:therapy; D002196:Capillaries / Q000473:pathology / Q000502:physiology; D002908:Chronic Disease; D004195:Disease Models, Animal; D005121:Extremities / Q000098:blood supply; D007511:Ischemia / Q000473:pathology / Q000628:therapy*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D009414:Nerve Growth Factors / Q000494:pharmacology / Q000502:physiology*; D000075388:Netrin-1; D058729:Peripheral Arterial Disease / Q000473:pathology / Q000628:therapy; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D025521:Tumor Suppressor Proteins / Q000494:pharmacology / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D009414:Nerve Growth Factors; C000617354:Ntn1 protein, rat; D025521:Tumor Suppressor Proteins; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D000075388:Netrin-1
keywords: Angiogenesis; Arteriosclerosis obliterans; Autologous mesenchymal stem cell; Chronic limb ischemia; Netrin-1; Peripheral arterial disease