title: Ectopic osteoid and bone formation by three calcium-phosphate ceramics in rats, rabbits and dogs.
abstract: Calcium phosphate ceramics with specific physicochemical properties have been shown to induce de novo bone formation upon ectopic implantation in a number of animal models. In this study we explored the influence of physicochemical properties as well as the animal species on material-induced ectopic bone formation. Three bioceramics were used for the study: phase-pure hydroxyapatite (HA) sintered at 1200°C and two biphasic calcium phosphate (BCP) ceramics, consisting of 60 wt.% HA and 40 wt.% TCP (β-Tricalcium phosphate), sintered at either 1100°C or 1200°C. 108 samples of each ceramic were intramuscularly implanted in dogs, rabbits, and rats for 6, 12, and 24 weeks respectively. Histological and histomorphometrical analyses illustrated that ectopic bone and/or osteoid tissue formation was most pronounced in BCP sintered at 1100°C and most limited in HA, independent of the animal model. Concerning the effect of animal species, ectopic bone formation reproducibly occurred in dogs, while in rabbits and rats, new tissue formation was mainly limited to osteoid. The results of this study confirmed that the incidence and the extent of material-induced bone formation are related to both the physicochemical properties of calcium phosphate ceramics and the animal model.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Liao
authors.initials: L
authors.lastname: Wang
authors.affiliation: The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing, China.
authors.forename: Bi
authors.initials: B
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Chongyun
authors.initials: C
authors.lastname: Bao
authors.affiliation: Department of Tissue Regeneration, MIRA-Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, the Netherlands.
authors.forename: Pamela
authors.initials: P
authors.lastname: Habibovic
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, China.
authors.forename: Xingdong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry*; D004285:Dogs; D017886:Durapatite / Q000737:chemistry; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D011817:Rabbits; D051381:Rats
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D017886:Durapatite; C020243:calcium phosphate