title: Substrate versatility of polyhydroxyalkanoate producing glycerol grown bacterial enrichment culture.
abstract: Waste-based polyhydroxyalkanoate (PHA) production by bacterial enrichments generally follows a three step strategy in which first the wastewater is converted into a volatile fatty acid rich stream that is subsequently used as substrate in a selector and biopolymer production units. In this work, a bacterial community with high biopolymer production capacity was enriched using glycerol, a non-fermented substrate. The substrate versatility and PHA production capacity of this community was studied using glucose, lactate, acetate and xylitol as substrate. Except for xylitol, very high PHA producing capacities were obtained. The PHA accumulation was comparable or even higher than with glycerol as substrate. This is the first study that established a high PHA content (≈70 wt%) with glucose as substrate in a microbial enrichment culture. The results presented in this study support the development of replacing pure culture based PHA production by bacterial enrichment cultures. A process where mixtures of substrates can be easily handled and the acidification step can potentially be avoided is described.
authors.affiliation: Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands; Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, Lope Gomez de Marzoa s/n, 15700, Santiago de Compostela, Spain. Electronic address: helenamoralejogarate@gmail.com.
authors.forename: Helena
authors.initials: H
authors.lastname: Moralejo-Gárate
authors.affiliation: Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands. Electronic address: R.Kleerebezem@tudelft.nl.
authors.forename: Robbert
authors.initials: R
authors.lastname: Kleerebezem
authors.affiliation: Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, Lope Gomez de Marzoa s/n, 15700, Santiago de Compostela, Spain. Electronic address: anuska.mosquera@usc.es.
authors.forename: Anuska
authors.initials: A
authors.lastname: Mosquera-Corral
authors.affiliation: Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, Lope Gomez de Marzoa s/n, 15700, Santiago de Compostela, Spain. Electronic address: joseluis.campos@usc.es.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Campos
authors.affiliation: Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, Lope Gomez de Marzoa s/n, 15700, Santiago de Compostela, Spain.
authors.forename: Tania
authors.initials: T
authors.lastname: Palmeiro-Sánchez
authors.affiliation: Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands. Electronic address: M.C.M.vanLoosdrecht@tudelft.nl.
authors.forename: Mark C M
authors.initials: MCM
authors.lastname: van Loosdrecht
mesh_terms: D001704:Biopolymers / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology*; D005990:Glycerol / Q000378:metabolism*; D054813:Polyhydroxyalkanoates / Q000378:metabolism*
chemical_list: D001704:Biopolymers; D054813:Polyhydroxyalkanoates; D005990:Glycerol
keywords: Bacterial enrichment; Bioplastic; Biopolymer; Glycerol