title: Unexpected structure for the N-terminal domain of hepatitis C virus envelope glycoprotein E1.
abstract: Hepatitis C virus (HCV) infection remains a major health problem worldwide. HCV entry into host cells and membrane fusion are achieved by two envelope glycoproteins, E1 and E2. We report here the 3.5-Å resolution crystal structure of the N-terminal domain of the HCV E1 ectodomain, which reveals a complex network of covalently linked intertwined homodimers that do not harbour the expected truncated class II fusion protein fold.
authors.affiliation: 1] Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Headington, Oxford OX3 7BN, UK [2].
authors.forename: Kamel
authors.initials: K
authors.lastname: El Omari
authors.affiliation: 1] Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Headington, Oxford OX3 7BN, UK [2].
authors.forename: Oleg
authors.initials: O
authors.lastname: Iourin
authors.affiliation: 1] Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Headington, Oxford OX3 7BN, UK [2].
authors.forename: Jan
authors.initials: J
authors.lastname: Kadlec
authors.affiliation: Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Headington, Oxford OX3 7BN, UK.
authors.forename: Geoff
authors.initials: G
authors.lastname: Sutton
authors.affiliation: Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Headington, Oxford OX3 7BN, UK.
authors.forename: Karl
authors.initials: K
authors.lastname: Harlos
authors.affiliation: 1] Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Headington, Oxford OX3 7BN, UK [2] Diamond Light Source Limited, Harwell Science and Innovation Campus, Didcot OX11 0DE, UK.
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Grimes
authors.affiliation: 1] Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Headington, Oxford OX3 7BN, UK [2] Diamond Light Source Limited, Harwell Science and Innovation Campus, Didcot OX11 0DE, UK.
authors.forename: David I
authors.initials: DI
authors.lastname: Stuart
mesh_terms: D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D015870:Gene Expression; D057809:HEK293 Cells; D016174:Hepacivirus / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D017510:Protein Folding; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: C087035:E1 protein, Hepatitis C virus; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins