title: Mycobacterium tuberculosis Erdman infection of rhesus macaques of Chinese origin.
abstract: Continuous high global tuberculosis (TB) mortality rates and variable vaccine efficacy of mycobacterium Bacille Calmette-Guérin (BCG) emphasize the need for improved vaccines and drugs against TB, which require clinically relevant animal models for evaluation. We infected a total of 24 Chinese rhesus macaques with varying doses (CFU of 25, 100 and 500) of Mycobacterium tuberculosis (M.tb) Erdman strain via bronchoscopy. Regardless of the M.tb doses, all animals were infected successfully with minor differences in clinical progression; as evidenced by clinical manifestations, laboratory analyses, bacterial burden in infected tissues and histopathology evaluations. Rhesus macaques of Chinese origin are highly susceptible to infection with M.tb Erdman strain and develop acute TB disease, which is similar to that in humans. Pathologically, Chinese rhesus macaques recapitulated the complete spectrum of granulomatous lesions seen in human TB disease. These data indicate that low-dose infection of rhesus macaques of Chinese origin is a suitable model for acute M.tb infection.
authors.affiliation: Animal Biosafety Level III Laboratory at the Center for Animal Experiment, Wuhan University School of Medicine, Wuhan, Hubei, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Animal Biosafety Level III Laboratory at the Center for Animal Experiment, Wuhan University School of Medicine, Wuhan, Hubei, PR China.
authors.forename: Qiaoyang
authors.initials: Q
authors.lastname: Xian
authors.affiliation: Animal Biosafety Level III Laboratory at the Center for Animal Experiment, Wuhan University School of Medicine, Wuhan, Hubei, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Guo
authors.affiliation: Animal Biosafety Level III Laboratory at the Center for Animal Experiment, Wuhan University School of Medicine, Wuhan, Hubei, PR China.
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Animal Biosafety Level III Laboratory at the Center for Animal Experiment, Wuhan University School of Medicine, Wuhan, Hubei, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Rao
authors.affiliation: Animal Biosafety Level III Laboratory at the Center for Animal Experiment, Wuhan University School of Medicine, Wuhan, Hubei, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Animal Biosafety Level III Laboratory at the Center for Animal Experiment, Wuhan University School of Medicine, Wuhan, Hubei, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Animal Biosafety Level III Laboratory at the Center for Animal Experiment, Wuhan University School of Medicine, Wuhan, Hubei, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Bao
authors.affiliation: Aeras, Rockville, MD, USA.
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Evans
authors.affiliation: Aeras, Rockville, MD, USA.
authors.forename: David
authors.initials: D
authors.lastname: Hokey
authors.affiliation: Aeras, Rockville, MD, USA.
authors.forename: Donata
authors.initials: D
authors.lastname: Sizemore
authors.affiliation: Animal Biosafety Level III Laboratory at the Center for Animal Experiment, Wuhan University School of Medicine, Wuhan, Hubei, PR China; Department of Pathology and Laboratory Medicine, Temple University School of Medicine, Philadelphia, PA, USA. Electronic address: wenzheho@whu.edu.cn.
authors.forename: Wen-Zhe
authors.initials: WZ
authors.lastname: Ho
mesh_terms: D000208:Acute Disease; D000818:Animals; D001799:Blood Sedimentation; D001999:Bronchoscopy; D002097:C-Reactive Protein / Q000378:metabolism; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D005260:Female; D008253:Macaca mulatta; D008297:Male; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000472:pathogenicity*; D011859:Radiography; D016019:Survival Analysis; D014374:Tuberculin Test; D014397:Tuberculosis, Pulmonary / Q000000981:diagnostic imaging / Q000382:microbiology* / Q000473:pathology; D014774:Virulence
chemical_list: D002097:C-Reactive Protein
keywords: Chinese rhesus macaques; Erdman strain; Mycobacterium tuberculosis; Non-human primate