title: Evaluation of the antioxidant activity of passion fruit (Passiflora edulis and Passiflora alata) extracts on stimulated neutrophils and myeloperoxidase activity assays.
abstract: The antioxidant activity of methanol extracts from Passiflora edulis and Passiflora alata pulp, and P. edulis rinds, healthy or infected with the passion fruit woodiness virus (PWV), was investigated using the oxidant activities of the neutrophil and the neutrophil granule enzyme myeloperoxidase (MPO), both playing key roles in inflammation. The reactive oxygen species produced by stimulated neutrophils were evaluated by lucigenin-enhanced chemiluminescence (CL) and the activity of purified MPO was measured by SIEFED (Specific Immunological Extraction Followed by Enzymatic Detection), a technique for studying the direct interaction of a compound with the enzyme. The rind extracts of P. edulis possessed higher and dose-dependent inhibitory effects on CL response and on the peroxidase activity of MPO than total pulp extracts from both passion fruit species. The quantification of isoorientin in the extracts showed a correlation with their antioxidant activity, suggesting the potential of P. edulis rinds as functional food or as a possible source of natural flavonoids.
authors.affiliation: Institute of Chemistry of São Carlos, University of São Paulo, P.O. Box 780, 13560-970 São Carlos, SP, Brazil.
authors.forename: Maria Luiza
authors.initials: ML
authors.lastname: Zeraik
authors.affiliation: Centre for Oxygen Research and Development (C.O.R.D.), Institute of Chemistry B6a, University of Liège, 4000 Liège, Belgium.
authors.forename: Didier
authors.initials: D
authors.lastname: Serteyn
authors.affiliation: Centre for Oxygen Research and Development (C.O.R.D.), Institute of Chemistry B6a, University of Liège, 4000 Liège, Belgium.
authors.forename: Ginette
authors.initials: G
authors.lastname: Deby-Dupont
authors.affiliation: Laboratory of Pharmacognosy, Institute of Pharmacy, University of Liège, CHU, B-4000 Liège 1, Belgium.
authors.forename: Jean-Nöel
authors.initials: JN
authors.lastname: Wauters
authors.affiliation: Laboratory of Pharmacognosy, Institute of Pharmacy, University of Liège, CHU, B-4000 Liège 1, Belgium.
authors.forename: Monique
authors.initials: M
authors.lastname: Tits
authors.affiliation: Institute of Chemistry of São Carlos, University of São Paulo, P.O. Box 780, 13560-970 São Carlos, SP, Brazil.
authors.forename: Janete H
authors.initials: JH
authors.lastname: Yariwake
authors.affiliation: Laboratory of Pharmacognosy, Institute of Pharmacy, University of Liège, CHU, B-4000 Liège 1, Belgium.
authors.forename: Luc
authors.initials: L
authors.lastname: Angenot
authors.affiliation: Centre for Oxygen Research and Development (C.O.R.D.), Institute of Chemistry B6a, University of Liège, 4000 Liège, Belgium. Electronic address: T.Franck@ulg.ac.be.
authors.forename: Thierry
authors.initials: T
authors.lastname: Franck
keywords: Antioxidant activity; Flavonoids; Isoorientin; Myeloperoxidase; Neutrophils; Passiflora alata Dryander; Passiflora edulis Sims f. flavicarpa Degener; Passion fruit