title: Dietary fermentable fiber upregulated immune related genes expression, increased innate immune response and resistance of rainbow trout (Oncorhynchus mykiss) against Aeromonas hydrophila.
abstract: This trial was carried out to investigate the effects of dietary administration of Vitacel(®), a commercial fermentable fiber, on immune related genes (Lysozyme, TNFα and HSP70) expression, innate immune response and resistance of rainbow trout against Aeromonas hydrophila. 120 healthy rainbow trout (81.65 ± 1.49 g) were distributed in six fiberglass tanks assigned to two treatments. The treatments were feeding rainbow trout with diets supplemented with 0 (control) or 10 g kg(-1) Vitacel(®) for 45 days. The results revealed that administration of fermentable fiber significantly (P < 0.05) upregulated lysozyme and TNFα gene expression. HSP70 gene expression was significantly lower in Vitacel(®) fed fish at the end of trial (P < 0.05). Furthermore dietary administrations of Vitacel(®) remarkably elevated rainbow trout innate immune parameters include serum lysozyme, ACH50, bactericidal activity and agglutination antibody titer (P < 0.05). Administration of 10 g kg(-1) Vitacel(®) significantly increased rainbow trout resistance against A. hydrophila (P < 0.05). The results of present study revealed that dietary Vitacel(®) can upregulates immune related genes expression and elevates innate immune response and disease resistance of rainbow trout.
authors.affiliation: Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Peyman
authors.initials: P
authors.lastname: Yarahmadi
authors.affiliation: Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Electronic address: kolangi@gau.ac.ir.
authors.forename: Hamed
authors.initials: H
authors.lastname: Kolangi Miandare
authors.affiliation: Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Farahmand
authors.affiliation: Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Mirvaghefi
authors.affiliation: Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Electronic address: hoseinifar@gau.ac.ir.
authors.forename: Seyed Hossein
authors.initials: SH
authors.lastname: Hoseinifar
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology*; D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D017931:DNA Primers / Q000235:genetics; D004043:Dietary Fiber / Q000494:pharmacology*; D060467:Disease Resistance / Q000187:drug effects / Q000276:immunology*; D005285:Fermentation; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000662:veterinary*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D007113:Immunity, Innate / Q000187:drug effects*; D009113:Muramidase / Q000378:metabolism; D017686:Oncorhynchus mykiss; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D017931:DNA Primers; D004043:Dietary Fiber; D018840:HSP70 Heat-Shock Proteins; D014409:Tumor Necrosis Factor-alpha; D009113:Muramidase
keywords: Disease resistance; Fermentable fiber; Immune genes expression; Immune response; Rainbow trout