title: The effect of peritoneal air exposure on intestinal mucosal barrier.
abstract: Background. Damage of the intestinal mucosa barrier may result in intestinal bacterial and endotoxin translocation, leading to local and systemic inflammation. The present study was designed to investigate whether peritoneal air exposure induces damage of intestinal mucosal barrier. Methods. Sprague-Dawley rats (weighing 210 to 230 g) were randomized into five groups (6/group): a control group, a sham group, and three exposure groups with peritoneal air exposure for 1, 2, and 3 h, respectively. At 24 h after surgery, blood and terminal ileum were sampled. The serum D-lactate levels were determined using an ELISA kit. The intestinal permeability was determined by measuring the intestinal clearance of FITC-dextran (FD4). The histopathological changes in terminal ileum were also assessed. Results. Compared with the controls, peritoneal air exposure caused an increase in both serum D-lactate level and intestinal FD4 clearance, which were proportional to the length of peritoneal air exposure and correlated to Chiu's scores, indices for intestinal mucosal injury. Edema and inflammatory cells were also observed in mucosa and submucosa of ileum in three exposure groups. Conclusions. Peritoneal air exposure could induce damage to the intestinal mucosal barrier, which is proportional to the time length of peritoneal air exposure.
authors.affiliation: Department of General Internal Medicine, Jiangsu Province Official Hospital, Nanjing 210024, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Bao
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China.
authors.forename: Shanjun
authors.initials: S
authors.lastname: Tan
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China.
authors.forename: Wenkui
authors.initials: W
authors.lastname: Yu
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China.
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China.
authors.forename: Qiyi
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China.
authors.forename: Jialiang
authors.initials: J
authors.lastname: Shi
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China.
authors.forename: Kaipeng
authors.initials: K
authors.lastname: Duan
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, Clinical School of Nanjing, Second Military Medical University, Nanjing 210002, China.
authors.forename: Xiaowu
authors.initials: X
authors.lastname: Bai
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, Clinical School of Nanjing, Second Military Medical University, Nanjing 210002, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China.
authors.forename: Jieshou
authors.initials: J
authors.lastname: Li
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China.
authors.forename: Ning
authors.identifier: 0000-0002-2042-3014
authors.initials: N
authors.lastname: Li