title: Quantitative analysis of P16 gene CpG methylation in Uyghur patients with cervical squamous cell carcinoma and its relationship with HPV16 infection.
abstract: The methylation of CpG sites in the promoter region of the P16 gene in Uyghur patients with cervical squamous cell carcinoma (CSCC) was quantitatively analyzed and its relationship with human papillomavirus 16 (HPV16) infection was explored. Cervical samples were collected from 20 Uyghur patients with CSCC and 20 Uyghur controls. Matrix-assisted laser desorption ionization-time of flight mass spectrometry was applied to detect methylation of CpG sites in the promoter region of the P16 gene; polymerase chain reaction was performed to assess HPV16 infection in the 2 groups. Among the 16 CpG sites in the P16 gene promoter region, the methylation level of the CpG1-2 and CpG 6 sites, as well as the HPV16 infection rate, was higher in the CSCC group than in the control group (P<0.05). There was no significant correlation between P16 CpG methylation and HPV16 infection in Uyghur patients with CSCC. The P16 gene CpG1-2 and CpG 6 hypermethylation and HPV16 infection, which are independent of each other, play an important role in cervical squamous cell carcinogenesis in Uyghur patients.
authors.affiliation: Department of Gynecology, Cancer Hospital Affiliated to Xinjiang Medical University, Urumchi, China.
authors.forename: J-X
authors.initials: JX
authors.lastname: Cheng
authors.affiliation: Department of Gynecology, Cancer Hospital Affiliated to Xinjiang Medical University, Urumchi, China.
authors.forename: M
authors.initials: M
authors.lastname: Yuan
authors.affiliation: Department of Gynecology, Cancer Hospital Affiliated to Xinjiang Medical University, Urumchi, China.
authors.forename: A-L
authors.initials: AL
authors.lastname: Li
authors.affiliation: Department of Gynecology, Cancer Hospital Affiliated to Xinjiang Medical University, Urumchi, China.
authors.forename: P
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Department of Gynecology, Cancer Hospital Affiliated to Xinjiang Medical University, Urumchi, China.
authors.forename: G-Q
authors.initials: GQ
authors.lastname: Shen
authors.affiliation: Department of Gynecology, Xiangya Hospital of Central South University, Changsha, China yizhangcn@yeah.net.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000209:etiology*; D002681:China; D016000:Cluster Analysis; D018899:CpG Islands; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D019175:DNA Methylation; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics; D006801:Humans; D030361:Papillomavirus Infections / Q000150:complications*; D002583:Uterine Cervical Neoplasms / Q000209:etiology*
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16