title: Biogeographical distribution of denitrifying anaerobic methane oxidizing bacteria in Chinese wetland ecosystems.
abstract: The discovery of denitrifying anaerobic methane oxidation with nitrite as electron acceptor mediated by 'Candidatus Methylomirabilis oxyfera' connected the biogeochemical carbon and nitrogen cycle in a new way. However, it is important to have a comprehensive understanding about the distribution of M. oxyfera-like bacteria in the terrestrial realm, especially the wetland ecosystems that are known as the largest natural source of atmospheric methane. Here, our molecular evidence demonstrated that a wide geographical distribution of M. oxyfera-like bacteria at oxic/anoxic interfaces of various wetlands (n = 91) over the Chinese territory. Intriguingly, the M. oxyfera-like bacteria were detected in some extreme environments, indicating that M. oxyfera-like bacteria occupied a wide range of habitats. Quantitative polymerase chain reaction estimated that the abundance of M. oxyfera-like bacteria ranged from 2.2 × 10(3) to 2.3 × 10(7) copies g(-1) dry soil, and up to around 0.62% of the total number of bacteria. Moreover, the M. oxyfera-like bacteria showed high biodiversity in wetland ecosystems based on the analysis of 462 pmoA and 287 16S rRNA gene sequences. The current study revealed the widespread distribution and biogeography of M. oxyfera-like bacteria in the terrestrial system.
authors.affiliation: Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
authors.forename: Guibing
authors.initials: G
authors.lastname: Zhu
authors.forename: Leiliu
authors.initials: L
authors.lastname: Zhou
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Shanyun
authors.initials: S
authors.lastname: Wang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Guo
authors.forename: Xi-En
authors.initials: XE
authors.lastname: Long
authors.forename: Xingbin
authors.initials: X
authors.lastname: Sun
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.forename: Qiaoyun
authors.initials: Q
authors.lastname: Hou
authors.forename: Mike S M
authors.initials: MS
authors.lastname: Jetten
authors.forename: Chengqing
authors.initials: C
authors.lastname: Yin
mesh_terms: D000693:Anaerobiosis; D044822:Biodiversity; D002681:China; D058440:Denitrification; D017753:Ecosystem; D008697:Methane / Q000378:metabolism; D008772:Methylococcaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D058458:Nitrogen Cycle; D010802:Phylogeny; D012988:Soil Microbiology; D053833:Wetlands
chemical_list: D008697:Methane