title: Complete sequence of a F2:A-:B- plasmid pHN3A11 carrying rmtB and qepA, and its dissemination in China.
abstract: Previous studies have confirmed that the spread of rmtB and qepA was mainly mediated by similar F2:A-:B- plasmids. In this study, a representative rmtB and qepA-harbouring F2:A-:B- plasmid, pHN3A11, originating from an Escherichia coli strain of feline origin, was fully sequenced and compared with other IncFII plasmids. pHN3A11 is 76,626bp long with a backbone similar to that of the IncFII plasmids obtained from China (pHK23a, pFOS-HK151325, pXZ) and Canada (pC15-1a). It contains genes encoding addiction (pemI/pemK, hok/mok/sok) and partitioning (parM, parB, and stbB) systems that promote plasmid maintenance during vertical transmission. rmtB, qepA, blaTEM-1, and dfr were found in previously observed contexts, interspersed with different complete or truncated insertion sequences and transposons (ΔIS1, ΔTn2, ΔintI1, ISCR3, 3 IS26, Tn21). Further analyses confirmed that pHN3A11-like plasmids have disseminated in E. coli isolates from pets, food animals and farm environments in China. The successful dissemination of F2:A-:B- type multidrug resistant plasmid among animals may represent a public health risk, and may further worsen the clinical impact.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; State Key Laboratory of Organic Geochemistry, CAS Centre for Pearl River Delta Environmental Pollution and Control Research, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, China.
authors.forename: Liangying
authors.initials: L
authors.lastname: He
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Yugu
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Zhenling
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Deng
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. Electronic address: gale@scau.edu.cn.
authors.forename: Yahong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. Electronic address: jhliu@scau.edu.cn.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002371:Cat Diseases / Q000453:epidemiology* / Q000382:microbiology*; D002415:Cats; D002681:China / Q000453:epidemiology; D019295:Computational Biology; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000235:genetics / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics*; D008780:Methyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D029968:Escherichia coli Proteins; C524438:QepA protein, E coli; D008780:Methyltransferases; C540682:RmtB protein, E coli
keywords: 16S rRNA methylase; Animal; Plasmid; Plasmid-mediated quinolone resistance (PMQR)