title: A mouse line for inducible and reversible silencing of specific neurons.
abstract: BACKGROUND
  Genetic methods for inducibly and reversibly inhibiting neuronal activity of specific neurons are critical for exploring the functions of neuronal circuits. The engineered human glycine receptor, called ivermectin (IVM)-gated silencing receptor (IVMR), has been shown to possess this ability in vitro.
  
  
  RESULTS
  Here we generated a mouse line, in which the IVMR coding sequence was inserted into the ROSA26 locus downstream of a loxP-flanked STOP cassette. Specific Cre-mediated IVMR expression was revealed by mis-expression of Cre in the striatum and by crossing with several Cre lines. Behavioral alteration was observed in Rosa26-IVMR mice with unilateral striatal Cre expression after systemic administration of IVM, and it could be re-initiated when IVM was applied again. A dramatic reduction in neuron firing was recorded in IVM-treated free moving Rosa26-IVMR;Emx1-Cre mice, and neuronal excitability was reduced within minutes as shown by recording in brain slice.
  
  
  CONCLUSIONS
  This Rosa26-IVMR mouse line provides a powerful tool for exploring selective circuit functions in freely behaving mice.
authors.forename: Ling
authors.initials: L
authors.lastname: Hu
authors.forename: Wei
authors.initials: W
authors.lastname: Lan
authors.forename: Hao
authors.initials: H
authors.lastname: Guo
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Chai
authors.forename: Kun
authors.initials: K
authors.lastname: Huang
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Ning-Ning
authors.initials: NN
authors.lastname: Song
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Bing
authors.initials: B
authors.lastname: Lang
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Qing-Xiu
authors.initials: QX
authors.lastname: Wang
authors.forename: Jin-Bao
authors.initials: JB
authors.lastname: Zhang
authors.forename: Collin
authors.initials: C
authors.lastname: McCaig
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.forename: Yu-Qiang
authors.initials: YQ
authors.lastname: Ding
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002460:Cell Line; D000229:Dependovirus / Q000378:metabolism; D005239:Fear; D020868:Gene Silencing / Q000187:drug effects; D018390:Gene Targeting; D056426:Genetic Loci; D049452:Green Fluorescent Proteins / Q000378:metabolism; D059765:Homologous Recombination / Q000235:genetics; D006801:Humans; D019426:Integrases / Q000378:metabolism; D007559:Ivermectin / Q000494:pharmacology; D008568:Memory / Q000187:drug effects; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D022661:RNA, Untranslated / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D022661:RNA, Untranslated; D011956:Receptors, Cell Surface; D049452:Green Fluorescent Proteins; D007559:Ivermectin; C045073:Cre recombinase; D019426:Integrases