title: Adenosine 5'-monophosphate-induced hypothermia inhibits the activation of ERK1/2, JNK, p38 and NF-κB in endotoxemic rats.
abstract: Many studies have shown that LPS mainly activates four signal transduction pathways to induce inflammation, namely the p38, ERK1/2, JNK and IKK/NF-κB pathways. Studies have demonstrated that 5'-AMP-induced hypothermia (AIH) exhibits high anti-inflammatory capabilities. In this study, we explore that how AIH inhibits the inflammatory response. Wistar rats were divided into five groups: a control group, an LPS group, a 5'-AMP pre-treatment group, a 5'-AMP post-treatment group and a 5'-AMP group. For each group, plasma and lung were collected from the rats at 6h and 12h after LPS injection. ELISA assays were used to detect plasma levels of CD14, CRP and MCP-1. Inflammatory pathway activation and TLR4 expression were assayed separately by Western blot analysis and immunohistochemistry. Our results showed that rats treated with AIH either before or after an LPS-challenge had a significant decrease in plasma levels of CD14, CRP and TLR4 compared with rats that received LPS only. Western blot analysis showed that AIH inhibited the activation of extracellular signal-regulated kinases (ERK) 1/2, p38, c-Jun N-terminal kinase (JNK) and NF-κB in inflammatory rats. Our study concluded that AIH attenuated LPS-induced inflammation mainly by inhibiting activation on the ERK1/2, p38, JNK and NF-κB signaling pathways.
authors.affiliation: Gout Laboratory, The Affiliated Hospital of Medical College Qingdao University, Shandong Provincial Key Laboratory of Metabolic Diseases, 16 Jiangsu Road, Qingdao, China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biochemistry, Medical College, Qingdao University, Qingdao, Shandong, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Stomatological Department, Qingdao Municipal Hospital, Qingdao, China.
authors.forename: Shulai
authors.initials: S
authors.lastname: Lu
authors.affiliation: ICU, The Affiliated Hospital of Medical College, 16 Jiangsu Road, Qingdao, China.
authors.forename: Xinting
authors.initials: X
authors.lastname: Pan
authors.affiliation: Pathology Department, Qingdao Municipal Hospital, Qingdao, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Jia
authors.affiliation: Pathology Department, The Affiliated Hospital of Medical College Qingdao University, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Yu
authors.affiliation: Pathology Department, The Affiliated Hospital of Medical College Qingdao University, China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Gout Laboratory, The Affiliated Hospital of Medical College Qingdao University, Shandong Provincial Key Laboratory of Metabolic Diseases, 16 Jiangsu Road, Qingdao, China.
authors.forename: Xinde
authors.initials: X
authors.lastname: Li
authors.affiliation: Gout Laboratory, The Affiliated Hospital of Medical College Qingdao University, Shandong Provincial Key Laboratory of Metabolic Diseases, 16 Jiangsu Road, Qingdao, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Cardiology, The Affiliated Hospital of Medical College Qingdao University, 16 Jiangsu Road, Qingdao, China.
authors.forename: Jidong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry, Medical College, Qingdao University, Qingdao, Shandong, China. Electronic address: qingyi001@126.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Hou
authors.affiliation: ICU, The Affiliated Hospital of Medical College, 16 Jiangsu Road, Qingdao, China. Electronic address: wylapollo@163.com.
authors.forename: Yunbo
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000249:Adenosine Monophosphate / Q000378:metabolism; D000818:Animals; D002097:C-Reactive Protein / Q000378:metabolism; D018932:Chemokine CCL2 / Q000097:blood; D004195:Disease Models, Animal; D019446:Endotoxemia / Q000139:chemically induced / Q000276:immunology* / Q000628:therapy; D004789:Enzyme Activation; D006801:Humans; D007036:Hypothermia, Induced; D018950:Lipopolysaccharide Receptors / Q000097:blood; D008070:Lipopolysaccharides / Q000008:administration & dosage; D020935:MAP Kinase Signaling System; D016328:NF-kappa B / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism
chemical_list: D018932:Chemokine CCL2; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D016328:NF-kappa B; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4; D000249:Adenosine Monophosphate; D002097:C-Reactive Protein
keywords: Adenosine 5′-monophosphate; Endotoxemia; Hypothermia; Inflammation