title: The wheat E subunit of V-type H+-ATPase is involved in the plant response to osmotic stress.
abstract: The vacuolar type H+-ATPase (V-type H+-ATPase) plays important roles in establishing an electrochemical H+-gradient across tonoplast, energizing Na+ sequestration into the central vacuole, and enhancing salt stress tolerance in plants. In this paper, a putative E subunit of the V-type H+-ATPase gene, W36 was isolated from stress-induced wheat de novo transcriptome sequencing combining with 5'-RACE and RT-PCR methods. The full-length of W36 gene was 1097 bp, which contained a 681 bp open reading frame (ORF) and encoded 227 amino acids. Southern blot analysis indicated that W36 was a single-copy gene. The quantitative real-time PCR (qRT-PCR) analysis revealed that the expression level of W36 could be upregulated by drought, cold, salt, and exogenous ABA treatment. A subcellular localization assay showed that the W36 protein accumulated in the cytoplasm. Isolation of the W36 promoter revealed some cis-acting elements responding to abiotic stresses. Transgenic Arabidopsis plants overexpressing W36 were enhanced salt and mannitol tolerance. These results indicate that W36 is involved in the plant response to osmotic stress.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling 712100, China. zhxh2493@126.com.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling 712100, China. libo708@126.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling 712100, China. huyingang@126.com.
authors.forename: Yin-Gang
authors.initials: YG
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling 712100, China. chenliang9117@nwafu.edu.cn.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling 712100, China. mdh2493@nwsuaf.edu.cn.
authors.forename: Dong-Hong
authors.initials: DH
authors.lastname: Min
mesh_terms: D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D000613:Aminobutyrates / Q000494:pharmacology; D017360:Arabidopsis / Q000201:enzymology; D055864:Droughts; D008353:Mannitol / Q000494:pharmacology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009997:Osmotic Pressure; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D012965:Sodium Chloride / Q000494:pharmacology; D014908:Triticum / Q000201:enzymology*; D015854:Up-Regulation / Q000187:drug effects; D025262:Vacuolar Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*
chemical_list: D020121:5' Untranslated Regions; D000613:Aminobutyrates; D010940:Plant Proteins; D021122:Protein Subunits; D008353:Mannitol; D012965:Sodium Chloride; D025262:Vacuolar Proton-Translocating ATPases