title: Transcriptomics reveal several gene expression patterns in the piezophile Desulfovibrio hydrothermalis in response to hydrostatic pressure.
abstract: RNA-seq was used to study the response of Desulfovibrio hydrothermalis, isolated from a deep-sea hydrothermal chimney on the East-Pacific Rise at a depth of 2,600 m, to various hydrostatic pressure growth conditions. The transcriptomic datasets obtained after growth at 26, 10 and 0.1 MPa identified only 65 differentially expressed genes that were distributed among four main categories: aromatic amino acid and glutamate metabolisms, energy metabolism, signal transduction, and unknown function. The gene expression patterns suggest that D. hydrothermalis uses at least three different adaptation mechanisms, according to a hydrostatic pressure threshold (HPt) that was estimated to be above 10 MPa. Both glutamate and energy metabolism were found to play crucial roles in these mechanisms. Quantitation of the glutamate levels in cells revealed its accumulation at high hydrostatic pressure, suggesting its role as a piezolyte. ATP measurements showed that the energy metabolism of this bacterium is optimized for deep-sea life conditions. This study provides new insights into the molecular mechanisms linked to hydrostatic pressure adaptation in sulfate-reducing bacteria.
authors.affiliation: Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM110, Marseille, France; Aix-Marseille Université, CNRS, LCB-UMR7283, Marseille, France.
authors.forename: Amira
authors.initials: A
authors.lastname: Amrani
authors.affiliation: Inserm, U1090, TGML/TAGC, Marseille, France; Aix Marseille Université, UMR_S 1090, TGML/TAGC, Marseille, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Bergon
authors.affiliation: Inserm, U1090, TGML/TAGC, Marseille, France; Aix Marseille Université, UMR_S 1090, TGML/TAGC, Marseille, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Holota
authors.affiliation: Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM110, Marseille, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Tamburini
authors.affiliation: Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM110, Marseille, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Garel
authors.affiliation: Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM110, Marseille, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Ollivier
authors.affiliation: Inserm, U1090, TGML/TAGC, Marseille, France; Aix Marseille Université, UMR_S 1090, TGML/TAGC, Marseille, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Imbert
authors.affiliation: Aix-Marseille Université, CNRS, LCB-UMR7283, Marseille, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Dolla
authors.affiliation: Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM110, Marseille, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Pradel
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000235:genetics; D003901:Desulfovibrio / Q000235:genetics*; D004734:Energy Metabolism / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics; D018698:Glutamic Acid / Q000378:metabolism; D006874:Hydrostatic Pressure; D020411:Oligonucleotide Array Sequence Analysis; D017423:Sequence Analysis, RNA; D059467:Transcriptome / Q000235:genetics*
chemical_list: D018698:Glutamic Acid; D000255:Adenosine Triphosphate