title: Quantitative neuropeptidomics study of the effects of temperature change in the crab Cancer borealis.
abstract: Temperature changes influence the reaction rates of all biological processes, which can pose dramatic challenges to cold-blooded organisms, and the capability to adapt to temperature fluctuations is crucial for the survival of these animals. In order to understand the roles that neuropeptides play in the temperature stress response, we employed a mass spectrometry-based approach to investigate the neuropeptide changes associated with acute temperature elevation in three neural tissues from the Jonah crab Cancer borealis. At high temperature, members from two neuropeptide families, including RFamide and RYamide, were observed to be significantly reduced in one of the neuroendocrine structures, the pericardial organ, while several orcokinin peptides were detected to be decreased in another major neuroendocrine organ, the sinus gland. These results implicate that the observed neuropeptides may be involved with temperature perturbation response via hormonal regulation. Furthermore, a temperature stress marker peptide with the primary sequence of SFRRMGGKAQ (m/z 1137.7) was detected and de novo sequenced in the circulating fluid (hemolymph) from animals under thermal perturbation.
authors.affiliation: Research Center of Basic Medical Sciences, Tianjin Medical University , Tianjin 300070, China.
authors.forename: Ruibing
authors.initials: R
authors.lastname: Chen
authors.forename: Mingming
authors.initials: M
authors.lastname: Xiao
authors.forename: Amanda
authors.initials: A
authors.lastname: Buchberger
authors.forename: Lingjun
authors.initials: L
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000302:isolation & purification / Q000378:metabolism*; D003386:Brachyura / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism; D009479:Neuropeptides / Q000302:isolation & purification / Q000378:metabolism*; D009490:Neurosecretory Systems / Q000378:metabolism; D010496:Pericardium / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature
chemical_list: D060829:Arthropod Proteins; D009479:Neuropeptides; C079067:orcokinin; C058047:arginylphenylalaninamide
keywords: Cancer borealis; MALDI-FTMS; MALDI-TOF/TOF; dimethyl labeling; mass spectrometry; neuropeptide; pericardial organ; quantitative peptidomics; sinus gland; temperature change