title: Highly selective colorimetric detection and estimation of Hg2+ at nano-molar concentration by silver nanoparticles in the presence of glutathione.
abstract: The present study investigated the colorimetric detection of mercury (Hg(2+)) ions by using silver nanoparticles (Ag NPs) in the presence of glutathione. The nanoparticles used in the study were synthesized biologically by using Polyalthia longifolia leaf extract. The synthesized nanoparticles were characterized by UV-visible spectrophotometer, transmission electron microscope, X-ray diffraction, particle size analyzer and zeta sizer. The particles were spherical in shape and it possesses the effective diameter of 5 nm. The zeta potential of the particles was determined to be -28.6 mV. Ag NPs-glutathione conjugates were able to detect Hg(2+) in nanomolar level. Ag NPs-glutathione conjugates upon interaction with Hg(2+) changes from yellowish brown to pale yellow and finally colorless. The study may be applied for the qualitative and quantitative estimation of mercury at very low concentration.
authors.affiliation: Centre for Nanotechnology and Advanced Biomaterials, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613 401, Tamil Nadu, India.
authors.forename: Ayesha
authors.initials: A
authors.lastname: Alam
authors.affiliation: Centre for Nanotechnology and Advanced Biomaterials, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613 401, Tamil Nadu, India.
authors.forename: Aswathy
authors.initials: A
authors.lastname: Ravindran
authors.affiliation: Centre for Nanotechnology and Advanced Biomaterials, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613 401, Tamil Nadu, India. Electronic address: preethypreethus@gmail.com.
authors.forename: Preethy
authors.initials: P
authors.lastname: Chandran
authors.affiliation: Centre for Nanotechnology and Advanced Biomaterials, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613 401, Tamil Nadu, India. Electronic address: ssudheerkhan@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Sudheer Khan
mesh_terms: D003124:Colorimetry / Q000379:methods*; D005978:Glutathione / Q000737:chemistry*; D055772:Green Chemistry Technology; D008628:Mercury / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D031044:Polyalthia / Q000737:chemistry; D012680:Sensitivity and Specificity; D012834:Silver; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D010936:Plant Extracts; D012834:Silver; D008628:Mercury; D005978:Glutathione
keywords: Biosynthesis; Colorimetric detection; Glutathione; Mercury; Silver nanoparticles