title: Expression of recombinant proteins by BEVS in a macula-like virus-free silkworm cell line.
abstract: We previously established the first Bombyx mori macula-like virus (BmMLV)-free cell line (BmVF cells) from a B. mori embryo. In this study, we evaluated the expression of recombinant proteins in BmVF cells using a B. mori nucleopolyhedrovirus (BmNPV)-derived expression vector. Our results showed that BmVF cells are susceptible to BmNPV, and both the promoter activity of the polyhedrin gene and the post-translated modifications of a recombinant protein are equivalent between BmMLV-negative BmVF and -positive BmN4 cells. These findings indicate that persistent infection with BmMLV has no discernible effect on BmNPV-mediated protein production in B. mori cells.
authors.affiliation: Department of Agrobiology and Bioresources, Faculty of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya-shi, Tochigi 321-8505, Japan. Electronic address: iwanaga@cc.utsunomiya-u.ac.jp.
authors.forename: Masashi
authors.initials: M
authors.lastname: Iwanaga
authors.affiliation: Department of Agrobiology and Bioresources, Faculty of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya-shi, Tochigi 321-8505, Japan.
authors.forename: Keita
authors.initials: K
authors.lastname: Tsukui
authors.affiliation: Department of Agrobiology and Bioresources, Faculty of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya-shi, Tochigi 321-8505, Japan.
authors.forename: Koudai
authors.initials: K
authors.lastname: Uchiyama
authors.affiliation: Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Susumu
authors.initials: S
authors.lastname: Katsuma
authors.affiliation: Genebank, National Institute of Agrobiological Science, Kannondai 2-1-2, Tsukuba-shi, Ibaraki 305-8602, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Imanishi
authors.affiliation: Department of Agrobiology and Bioresources, Faculty of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya-shi, Tochigi 321-8505, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Kawasaki
mesh_terms: D000818:Animals; D015153:Blotting, Western; D012831:Bombyx / Q000821:virology*; D002460:Cell Line; D017924:Nucleopolyhedroviruses; D011994:Recombinant Proteins / Q000096:biosynthesis; D046830:Tymoviridae; D014773:Virology / Q000379:methods*
chemical_list: D011994:Recombinant Proteins
keywords: BEVS; Baculovirus; BmMLV; BmNPV; Maculavirus; Nucleopolyhedrovirus