title: Flavonol glycosides in the petal of Rosa species as chemotaxonomic markers.
abstract: Thirteen flavonol glycosides were isolated from the petals of Rosa species belonging to the section Gallicanae, and their structures were identified from their spectroscopic data. These flavonol glycosides, along with two flavonol glycosides isolated from Rosa rugosa, in the petals of 31 Rosa species belonging to sections Gallicanae, Cinnamomeae, Caninae, and Synstylae were quantitatively analyzed by UPLC. The results indicated that the species belonging to these sections could be classified into four types (Type A, B, C and D) based on the pattern of flavonol glycoside contents, whereas the R. rugosa flavonol glycosides were detected only in section Cinnamomeae. A principal components analysis (PCA) calculated from the 15 flavonol glycosides contained in these samples supported the presence of four types. The distribution of the species in Type D (a group of Cinnamomeae) was shown to reflect close interrelationships, but species in Type B (one group of Gallicanae) could be subdivided into two groups, one of which contained species in section Synstylae. Moreover, the flavonol glycosides were grouped by sugar moieties: a disaccharide composed of two hexoses (S1), a hexose (S2), including a hexose with galloyl group, a pentose (S3), and a disaccharide composed of a hexose and a pentose (S4). The ratios of the amounts of S1-S4 to total flavonol glycoside content indicated that differences among the four sections were more distinctive than the amounts of the 15 flavonol glycosides. The 31 samples were divided into Type B, composed of one type of Gallicanae and Synstylae, Type A+C, composed of another type of Gallicanae and Caninae, and Type D, composed of Cinnamomeae. The R. rugosa flavonol glycosides were shown to be important chemotaxonomic markers for the classification of species in Cinnamomeae, and this method of using flavonol glycosides as chemotaxonomic markers could be useful for the identification of Rosa species belonging to sections Gallicanae, Cinnamomeae, Caninae, and Synstylae.
authors.affiliation: Kitami Institute of Technology, 165 Koen-cho, Kitami 090-8507, Japan.
authors.forename: Ochir
authors.initials: O
authors.lastname: Sarangowa
authors.affiliation: Kitami Institute of Technology, 165 Koen-cho, Kitami 090-8507, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kanazawa
authors.affiliation: Faculty of Bio-Industry, Tokyo University of Agriculture, 196 Yasaka, Abashiri 099-2493, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Nishizawa
authors.affiliation: Faculty of Bio-Industry, Tokyo University of Agriculture, 196 Yasaka, Abashiri 099-2493, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Myoda
authors.affiliation: Academy of Mongolian Medicine, Inner Mongolia Medical University, Hohhot 010110, China.
authors.forename: Changxi
authors.initials: C
authors.lastname: Bai
authors.affiliation: Kitami Institute of Technology, 165 Koen-cho, Kitami 090-8507, Japan. Electronic address: yamagta@mail.kitami-it.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamagishi
mesh_terms: D044948:Flavonols / Q000032:analysis / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D035264:Flowers / Q000737:chemistry; D006027:Glycosides / Q000032:analysis / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification*; D044949:Kaempferols / Q000737:chemistry; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011794:Quercetin / Q000737:chemistry; D027826:Rosa / Q000737:chemistry*
chemical_list: D044948:Flavonols; D006027:Glycosides; D044949:Kaempferols; C006552:kaempferol; D011794:Quercetin
keywords: Chemotaxonomy; Kaempferol glycoside; Quercetin glycoside; Rosa gallica; Rosa rugosa; Rosa species; Rosaceae