title: MiR-124 represses vasculogenic mimicry and cell motility by targeting amotL1 in cervical cancer cells.
abstract: miRNAs have extensive functions in differentiation, metabolism, programmed cell death, and tumor metastasis by post-transcriptional regulation. Vasculogenic mimicry is an important pathway in tumor metastasis. Many factors can regulate vasculogenic mimicry, including miRNAs. In previous studies, miR-124 was found to repress proliferation and metastasis in different types of cancers, but whether it functions in cervical cancer remained unknown. Here, we demonstrate that miR-124 can repress vasculogenic mimicry, migration and invasion in HeLa and C33A cells in vitro. Furthermore, we reveal that the effect of miR-124 on vasculogenic mimicry, migration and invasion results from its interaction with AmotL1. MiR-124 regulates AmotL1 negatively by targeting its 3'untranslated region (3'UTR). We found that miR-124 can repress the EMT process. Together, these results improve our understanding of the function of miR-124 in tumor metastasis and will help to provide new potential target sites for cervical cancer treatment.
authors.affiliation: Tianjin Life Science Research Center and School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Wan
authors.affiliation: The Department of Laboratory Animal Science and Technology, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Qin-Qin
authors.initials: QQ
authors.lastname: Li
authors.affiliation: Tianjin Life Science Research Center and School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Tianjin Life Science Research Center and School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tian
authors.affiliation: The Department of Laboratory Animal Science and Technology, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Li
authors.affiliation: Tianjin Life Science Research Center and School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Tianjin Life Science Research Center and School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Tianjin Life Science Research Center and School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China. Electronic address: htang2002@yahoo.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Tang
mesh_terms: D020413:3' Untranslated Regions; D000091082:Angiomotins; D001665:Binding Sites; D002277:Carcinoma / Q000098:blood supply / Q000235:genetics / Q000378:metabolism* / Q000556:secondary; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D018716:Molecular Mimicry; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic; D015398:Signal Transduction; D014162:Transfection; D002583:Uterine Cervical Neoplasms / Q000098:blood supply / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; C510063:AMOTL1 protein, human; D000091082:Angiomotins; C509207:MIRN124 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs
keywords: AmotL1; Cervical cancer cells; EMT; Vasculogenic mimicry; miR-124