title: [Study of immunomodulatory activity of progesterone in an experimental model of Jurkat cells].
abstract: Progesterone plays important the role in the regulation of the immune system during pregnancy. We investigated the influence of progesterone on the activity of T-lymphocytes in a model system of Jurkat cells. Jurkat cells were stimulated with 50 μg/ml of phytohemaglutinin A (PHA) at 370C for 5 minutes. Then, PHA was removed by centrifugation, the cells were washed and cultured for 24 hours alone or with progesterone (added to the incubation medium of Jurkat cells at a concentration of 0.07 and 0.7 μl). The effect of progesterone on the level of apoptosis and expression of cytokines in jointly incubated intact and PHA-stimulated Jurkat cells. It was revealed that progesteron did not affect the intensity of apoptosis and cytokine expression in intact, but dose-dependently promotes intensification of apoptosis (to 88%) and inhibits the cytotoxic activity of jointly incubated intact and PHA-stimulated Jurkat cells (ratio of IL-2/IL-10 decreased from 2.56 to 1.53).
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D016753:Interleukin-10 / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D019169:Jurkat Cells / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology; D011374:Progesterone / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
chemical_list: D016207:Cytokines; C508609:IL10 protein, human; D007155:Immunologic Factors; D007376:Interleukin-2; D010835:Phytohemagglutinins; D016753:Interleukin-10; D011374:Progesterone