title: [Expression of endogenous leukemia inhibitory factor in neonatal rats with periventricular leukomalacia].
abstract: OBJECTIVE
  To study the changes of endogenous leukemia inhibitory factor (LIF) in neonatal rats with periventricular leukomalacia (PVL).
  
  
  METHODS
  A PVL model of 3-day-old Wistar rats was prepared by left carotid artery ligation followed by 6% oxygen for 4 hours. The rats were sacrificed at 1, 3, 7, 14 and 28 days of hypoxia ischemia (HI), and the brain tissues were sampled. Real-Time PCR and Western blot methods were applied to analyze the expression of LIF mRNA and protein. Double staining immunofluorescence was used to detect the co-expression of LIF and GFAP.
  
  
  RESULTS
  At 1, 3 and 7 days of HI, LIF protein level in the PVL group was higher than in the control group (P<0.01). In the PVL group, the LIF protein level on the third day after HI reached a peak and was higher than the other time points (P<0.01). The change of LIF mRNA expression showed the same tendency with LIF protein. The double staining immunofluorescence showed a co-expression of LIF and GFAP.
  
  
  CONCLUSIONS
  LIF mRNA and LIF protein expression in astrocytes show a trend of initial increase followed by steady decline in neonatal rats with PVL, suggesting that endogenous LIF may participate in the repair of PVL.
authors.affiliation: Department of Pediatric Neurology, Shengjing Hospital of China Medical University, Shenyang 110004, China. wangh1@sj-hospital.org.
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Fan
authors.forename: Tao
authors.initials: T
authors.lastname: Yu
authors.forename: Jun-Mei
authors.initials: JM
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Gui-Feng
authors.initials: GF
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004195:Disease Models, Animal; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D053605:Leukemia Inhibitory Factor / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D007969:Leukomalacia, Periventricular / Q000378:metabolism* / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005904:Glial Fibrillary Acidic Protein; D053605:Leukemia Inhibitory Factor; D012333:RNA, Messenger