title: Development of highly sensitive amperometric biosensor for glucose using carbon nanosphere/sodium alginate composite matrix for enzyme immobilization.
abstract: In this study, we developed a highly sensitive amperometric biosensor for glucose detection based on glucose oxidase immobilized in a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix. This hybrid material combined the advantages of CNS and natural biopolymer SA. This composite film was characterized by scanning electron microscope, electrochemical impedance spectroscopy and UV-vis, which indicated that the hybrid material was suitable for immobilization of glucose oxidase. Various experimental conditions were investigated that influenced the performance of the biosensor, such as pH, applied potential and temperature. Under the optimum conditions, the biosensor showed excellent performance for glucose over a wide linear concentration range from 1.0 × 10(-6) to 4.6 × 10(-3) M with a detection limit of 0.5 μM based on a signal-to-noise ratio of 3. Furthermore, the biosensor exhibited excellent long-term stability and satisfactory reproducibility.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University.
authors.forename: En
authors.initials: E
authors.lastname: Han
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Cai
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Cui
authors.forename: Xing-Ai
authors.initials: XA
authors.lastname: Zhang
mesh_terms: D000464:Alginates / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D058266:Dielectric Spectroscopy; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D054118:Nanospheres / Q000737:chemistry*; D015203:Reproducibility of Results; D059629:Signal-To-Noise Ratio; D013056:Spectrophotometry, Ultraviolet; D013499:Surface Properties; D013997:Time Factors
chemical_list: D000464:Alginates; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D002244:Carbon; D020723:Glucuronic Acid; D005949:Glucose Oxidase; D005947:Glucose