title: Collection and extraction of saliva DNA for next generation sequencing.
abstract: The preferred source of DNA in human genetics research is blood, or cell lines derived from blood, as these sources yield large quantities of high quality DNA. However, DNA extraction from saliva can yield high quality DNA with little to no degradation/fragmentation that is suitable for a variety of DNA assays without the expense of a phlebotomist and can even be acquired through the mail. However, at present, no saliva DNA collection/extraction protocols for next generation sequencing have been presented in the literature. This protocol optimizes parameters of saliva collection/storage and DNA extraction to be of sufficient quality and quantity for DNA assays with the highest standards, including microarray genotyping and next generation sequencing.
authors.affiliation: Battelle Center for Mathematical Medicine, The Research Institute at Nationwide Children's Hospital.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Goode
authors.affiliation: Battelle Center for Mathematical Medicine, The Research Institute at Nationwide Children's Hospital.
authors.forename: Soo Yeon
authors.initials: SY
authors.lastname: Cheong
authors.affiliation: Battelle Center for Mathematical Medicine, The Research Institute at Nationwide Children's Hospital.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Battelle Center for Mathematical Medicine, The Research Institute at Nationwide Children's Hospital; The Interdisciplinary Graduate Program in Biophysics, The Ohio State University.
authors.forename: William C
authors.initials: WC
authors.lastname: Ray
authors.affiliation: Battelle Center for Mathematical Medicine, The Research Institute at Nationwide Children's Hospital; Department of Pediatrics, The Ohio State University; christopher.bartlett@nationwidechildrens.org.
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Bartlett
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D006801:Humans; D012463:Saliva / Q000737:chemistry*; D013048:Specimen Handling / Q000379:methods
chemical_list: D004247:DNA