title: Genotypic and phenotypic detection of efflux pump in Rhodococcus equi.
abstract: The req_39680 gene, associated to a putative efflux system, was detected in 60% (54/90) of R. equi isolates by PCR. The phenotypic expression of efflux mechanism was verified in 20% of the isolates using ethidium bromide. For the first time, the expression of efflux mechanism was demonstrated in R. equi.
authors.affiliation: Departamento de Medicina Veterinária Preventiva Universidade Federal de Santa Maria Santa MariaRS Brazil Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Letícia Trevisan
authors.initials: LT
authors.lastname: Gressler
authors.affiliation: Departamento de Medicina Veterinária Preventiva Universidade Federal de Santa Maria Santa MariaRS Brazil Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Agueda Castagna
authors.initials: AC
authors.lastname: de Vargas
authors.affiliation: Laboratório de Imunologia e Microbiologia Universidade Federal do Vale do São Francisco PetrolinaPE Brazil Laboratório de Imunologia e Microbiologia, Universidade Federal do Vale do São Francisco, Petrolina, PE, Brazil.
authors.forename: Mateus Matiuzzi
authors.initials: MM
authors.lastname: da Costa
authors.affiliation: Departamento de Zootecnia Universidade Federal de Santa Maria Santa MariaRS Brazil Departamento de Zootecnia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Luciana
authors.initials: L
authors.lastname: Pötter
authors.affiliation: Departamento de Medicina Veterinária Preventiva Universidade Federal de Santa Maria Santa MariaRS Brazil Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Bibiana Petri
authors.initials: BP
authors.lastname: da Silveira
authors.affiliation: Departamento de Medicina Veterinária Preventiva Universidade Federal de Santa Maria Santa MariaRS Brazil Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Luis Antônio
authors.initials: LA
authors.lastname: Sangioni
authors.affiliation: Departamento de Medicina Veterinária Preventiva Universidade Federal de Santa Maria Santa MariaRS Brazil Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Sônia
authors.initials: S
authors.lastname: de Avila Botton
mesh_terms: D001693:Biological Transport, Active; D004269:DNA, Bacterial / Q000235:genetics; D004996:Ethidium / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D016929:Rhodococcus equi / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D026901:Membrane Transport Proteins; D004996:Ethidium
keywords: Rhodococcus equi; efflux pump; ethidium bromide; multidrug resistance; vapA