title: Bioinformatics based structural characterization of glucose dehydrogenase (gdh) gene and growth promoting activity of Leclercia sp. QAU-66.
abstract: Glucose dehydrogenase (GDH; EC 1.1. 5.2) is the member of quinoproteins group that use the redox cofactor pyrroloquinoline quinoine, calcium ions and glucose as substrate for its activity. In present study, Leclercia sp. QAU-66, isolated from rhizosphere of Vigna mungo, was characterized for phosphate solubilization and the role of GDH in plant growth promotion of Phaseolus vulgaris. The strain QAU-66 had ability to solubilize phosphorus and significantly (p ≤ 0.05) promoted the shoot and root lengths of Phaseolus vulgaris. The structural determination of GDH protein was carried out using bioinformatics tools like Pfam, InterProScan, I-TASSER and COFACTOR. These tools predicted the structural based functional homology of pyrroloquinoline quinone domains in GDH. GDH of Leclercia sp. QAU-66 is one of the main factor that involved in plant growth promotion and provides a solid background for further research in plant growth promoting activities.
authors.affiliation: Plant Genomics Lab Department of Plant Sciences Quaid-i-Azam University Islamabad Pakistan Plant Genomics Lab, Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Naveed
authors.affiliation: National Institute for Genomics & Advanced Biotechnology National Agricultural Research Centre Islamabad Pakistan National Institute for Genomics & Advanced Biotechnology, National Agricultural Research Centre, Islamabad, Pakistan.
authors.forename: Iftikhar
authors.initials: I
authors.lastname: Ahmed
authors.affiliation: Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo Japan Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Nauman
authors.initials: N
authors.lastname: Khalid
authors.affiliation: Plant Genomics Lab Department of Plant Sciences Quaid-i-Azam University Islamabad Pakistan Plant Genomics Lab, Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Abdul Samad
authors.initials: AS
authors.lastname: Mumtaz
mesh_terms: D016000:Cluster Analysis; D019295:Computational Biology; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D042843:Glucose 1-Dehydrogenase / Q000737:chemistry / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors; D027805:Phaseolus / Q000254:growth & development* / Q000382:microbiology*; D010758:Phosphorus / Q000378:metabolism; D010802:Phylogeny; D018517:Plant Roots / Q000254:growth & development; D018520:Plant Shoots / Q000254:growth & development; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011809:Quinones / Q000032:analysis; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D004269:DNA, Bacterial; D009414:Nerve Growth Factors; D011809:Quinones; D010758:Phosphorus; D042843:Glucose 1-Dehydrogenase
keywords: Leclercia sp.; PGPR; bioinformatics; glucose dehydrogenase; pyrroloquinoline quinone