title: Cutaneous manifestations of spotted fever rickettsial infections in the Central Province of Sri Lanka: a descriptive study.
abstract: BACKGROUND
  Characteristic skin lesions play a key role in clinical diagnosis of spotted fever group rickettsioses and this study describes these cutaneous manifestations along with basic histological features.
  
  
  RESULTS
  Study was conducted at Medical Unit, Teaching Hospital, Peradeniya, from November 2009 to October 2011, where a prospective data base of all rickettsial infections is maintained. Confirmation of diagnosis was made when IgM and IgG immunofluorescent antibody titre of 1/32 and >1/256 respectively. Of the 210 clinical cases, 134 had cutoff antibody titers for Rickettsia conorii antigen for confirmation. All these 134 patients had fever and skin rash, and of them 132(98%) had discrete maculopapular rash while eight (6%) had fern leaf type skin necrosis. Eight patients (6%) had healed tick bite marks. Average size of a skin lesion was 5 mm and rash involved 52% of body surface, distributed mainly in limbs and back of the chest. Generally the facial and leg skin was slightly oedematous particularly in old aged patients. Sixteen patients (12%) had pain and swelling of ankle joints where swelling extended to feet and leg. Biopsies from skin rash of six patients showed evidence of cutaneous vasculitis and of them, 247 bp region of the 17-kDa spotted fever group specific protein antigen was amplified using PCR.
  
  
  CONCLUSIONS
  A discrete maculopapular rash and occasional variations such as fern leaf shape necrosis and arthritis are found in spotted fever group. Histology found vasculitis as the pathology of these lesions.
authors.affiliation: Department of Parasitology, Faculty of Medicine and Allied Sciences, Rajarata University of Sri Lanka, Saliyapura, Sri Lanka.
authors.forename: Kosala
authors.initials: K
authors.lastname: Weerakoon
authors.affiliation: Department of Medicine, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka.
authors.forename: Senanayake A M
authors.initials: SA
authors.lastname: Kularatne
authors.affiliation: Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka.
authors.forename: Jayanthe
authors.initials: J
authors.lastname: Rajapakse
authors.affiliation: Department of Anatomy, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka.
authors.forename: Sanjaya
authors.initials: S
authors.lastname: Adikari
authors.affiliation: Department of Pathology, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka.
authors.forename: Roshitha
authors.initials: R
authors.lastname: Waduge
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood; D001706:Biopsy; D005260:Female; D005334:Fever; D006801:Humans; D007074:Immunoglobulin G; D008297:Male; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D012281:Rickettsia; D012282:Rickettsia Infections / Q000453:epidemiology / Q000473:pathology*; D012867:Skin / Q000473:pathology; D017192:Skin Diseases, Bacterial / Q000473:pathology*; D013188:Sri Lanka / Q000453:epidemiology; D017282:Tick-Borne Diseases / Q000453:epidemiology / Q000473:pathology*
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G