title: Yamogenin in fenugreek inhibits lipid accumulation through the suppression of gene expression in fatty acid synthesis in hepatocytes.
abstract: Yamogenin is a diastereomer of diosgenin, which we have identified as the compound responsible for the anti-hyperlipidemic effect of fenugreek. Here, we examined the effects of yamogenin on the accumulation of triacylglyceride (TG) in hepatocytes, because yamogenin is also contained in fenugreek. It was demonstrated that yamogenin also inhibited TG accumulation in HepG2 hepatocytes and suppressed the mRNA expression of fatty acid synthesis-related genes such as fatty acid synthase and sterol response element-binding protein-1c. Indeed, yamogenin also antagonized the activation of the liver X receptor (LXR) in luciferase ligand assay similar to diosgenin. However, yamogenin could not exert such effects in the presence of T0901713, a potent agonist of LXR. These findings indicate that the effects of yamogenin on TG accumulation would be weaker than those of diosgenin, suggesting that the structural difference between yamogenin and diosgenin would be important for the inhibition of LXR activation.
authors.affiliation: a Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture , Kyoto University , Kyoto , Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Moriwaki
authors.forename: Hiroki
authors.initials: H
authors.lastname: Murakami
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Takahashi
authors.forename: Taku
authors.initials: T
authors.lastname: Uemura
authors.forename: Keiko
authors.initials: K
authors.lastname: Taketani
authors.forename: Shohei
authors.initials: S
authors.lastname: Hoshino
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Tsuge
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Narukami
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Goto
authors.forename: Teruo
authors.initials: T
authors.lastname: Kawada
mesh_terms: D000818:Animals; D004144:Diosgenin / Q000494:pharmacology*; D005227:Fatty Acids / Q000096:biosynthesis*; D005786:Gene Expression Regulation / Q000187:drug effects*; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects*; D000071518:Liver X Receptors; D008297:Male; D051379:Mice; D057093:Orphan Nuclear Receptors / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics; D014280:Triglycerides / Q000378:metabolism; D029910:Trigonella / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors; D012333:RNA, Messenger; D051780:Sterol Regulatory Element Binding Protein 1; D014280:Triglycerides; D004144:Diosgenin; C514916:yamogenin
keywords: LXR; diosgenin; hepatocyte; lipid metabolism; yamogenin