title: Differential contribution of the Fanconi anemia-related proteins to repair of several types of DNA damage in cultured silkworm cells.
abstract: The silkworm Fanconi anemia (FA) pathway is required for normal cellular resistance to mitomycin C (MMC) in silkworms, but little is known about the requirement for repair of other types of DNA damage. Here we report that silkworm cells deficient for FA proteins FancD2 and FancM exhibit normal sensitivities to hydroxyurea (HU) and camptothecin (CPT), although FancM-dependent FancD2 monoubiquitination is induced upon these treatments. Similar results were observed in cells depleted for Rmi1 and Mhf1, which interact with the FancM protein. We also found that Rad51-knockdown cells exhibited normal sensitivity to HU despite induction of double-strand breaks by HU treatment.
authors.affiliation: Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan; Insect Growth Regulation Research Unit, National Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan.
authors.forename: Ryohei
authors.initials: R
authors.lastname: Sugahara
authors.affiliation: Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Mon
authors.affiliation: Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
authors.forename: Jae Man
authors.initials: JM
authors.lastname: Lee
authors.affiliation: Insect Growth Regulation Research Unit, National Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Shiotsuki
authors.affiliation: Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan. Electronic address: kusakabe@agr.kyushu-u.ac.jp.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kusakabe
mesh_terms: D000818:Animals; D012831:Bombyx / Q000166:cytology*; D002166:Camptothecin / Q000494:pharmacology; D002478:Cells, Cultured; D004249:DNA Damage; D004265:DNA Helicases / Q000172:deficiency / Q000378:metabolism*; D004260:DNA Repair; D052236:Fanconi Anemia Complementation Group D2 Protein / Q000172:deficiency / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D059765:Homologous Recombination / Q000187:drug effects; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D016685:Mitomycin / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D054875:Ubiquitination / Q000187:drug effects
chemical_list: D052236:Fanconi Anemia Complementation Group D2 Protein; D006657:Histones; D016685:Mitomycin; D004265:DNA Helicases; D006918:Hydroxyurea; D002166:Camptothecin
keywords: BLM pathway; Camptothecin; DNA repair; Fanconi pathway; Homologous recombination; Hydroxyurea