title: MNAzymes provide a universal mechanism for triggering DNAzyme synthesis cascades.
abstract: Novel methods were developed to isothermally detect target nucleic acids and initiate signal amplification cascades. The methods utilize target-specific MNAzymes to activate universal primer molecules. These primers can subsequently promote the autonomous synthesis of DNAzymes capable of cleaving nucleic acid substrates and generating signal, which further creates the potential for circular feedback.
authors.affiliation: SpeeDx Pty Ltd, Eveleigh, New South Wales, 2015 Australia. simonb@speedx.com.au.
authors.forename: Simon M
authors.initials: SM
authors.lastname: Bone
authors.forename: Alison V
authors.initials: AV
authors.lastname: Todd
mesh_terms: D021881:DNA, Catalytic / Q000096:biosynthesis* / Q000378:metabolism*; D025461:Feedback, Physiological; D009696:Nucleic Acids / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*
chemical_list: D021881:DNA, Catalytic; D009696:Nucleic Acids