title: Effect of amines as activators on the alcohol-oxidizing activity of pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenase.
abstract: Pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenases (PQQ-ADH) require ammonia or primary amines as activators in in vitro assays with artificial electron acceptors. We found that PQQ-ADH from Pseudomonas putida KT2440 (PpADH) was activated by various primary amines, di-methylamine, and tri-methylamine. The alcohol oxidation activity of PpADH was strongly enhanced and the affinity for substrates was also improved by pentylamine as an activator.
authors.affiliation: a Department of Biotechnology and Life Science , Tokyo University of Agriculture and Technology , Koganei , Japan.
authors.forename: Kouta
authors.initials: K
authors.lastname: Takeda
authors.forename: Takuya
authors.initials: T
authors.lastname: Ishida
authors.forename: Kiyohiko
authors.initials: K
authors.lastname: Igarashi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Samejima
authors.forename: Nobuhumi
authors.initials: N
authors.lastname: Nakamura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohno
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000438:Alcohols / Q000378:metabolism*; D000588:Amines / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D045542:PQQ Cofactor / Q000378:metabolism*; D016958:Pseudomonas putida / Q000201:enzymology
chemical_list: D000438:Alcohols; D000588:Amines; D045542:PQQ Cofactor; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor)
keywords: alcohol dehydrogenase; alkyl amine; enzyme activator; pyrroloquinoline quinone; quinoprotein