title: Platelet-rich plasma encapsulation in hyaluronic acid/gelatin-BCP hydrogel for growth factor delivery in BCP sponge scaffold for bone regeneration.
abstract: Microporous calcium phosphate based synthetic bone substitutes are used for bone defect healing. Different growth factor loading has been investigated for enhanced bone regeneration. The platelet is a cellular component of blood which naturally contains a pool of necessary growth factors that mediate initiation, continuation, and completion of cellular mechanism of healing. In this work, we have investigated the encapsulation and immobilization of platelet-rich plasma (PRP) with natural polymers like hyaluronic acid (HA) and gelatin (Gel) and loading them in a biphasic calcium phosphate (BCP) scaffold, for a synthetic-allologous hybrid scaffold. Effect of PRP addition in small doses was evaluated for osteogenic potential in vitro and in vivo. BCP (10%) mixed HA-Gel hydrogel with or without PRP, was loaded into a BCP sponge scaffold. We investigated the hydrogel-induced improvement in mechanical property and PRP-mediated enhancement in biocompatibility. In vitro studies for cytotoxicity, cell attachment, and proliferation were carried out using MC3T3-E1 pre-osteoblast cells. In in vitro studies, the cell count, cell proliferation, and cell survival were higher in the scaffold with PRP loading than without PRP. However, in the in vivo studies using a rat model, the PRP scaffold was not superior to the scaffold without PRP. This discrepancy was investigated in terms of the interaction of PRP in the in vivo environment.
authors.affiliation: Department of Regenerative Medicine, Ssangyong-dong, Chungnam, Republic of Korea.
authors.forename: So-Ra
authors.initials: SR
authors.lastname: Son
authors.affiliation: Instititue of Tissue Regeneration, College of Medicine, Soonchunhyang University, Ssangyong-dong, Chungnam, Republic of Korea.
authors.forename: Swapan Kumar
authors.initials: SK
authors.lastname: Sarkar
authors.affiliation: Instititue of Tissue Regeneration, College of Medicine, Soonchunhyang University, Ssangyong-dong, Chungnam, Republic of Korea.
authors.forename: Ba Linh
authors.initials: BL
authors.lastname: Nguyen-Thuy
authors.affiliation: Department of Regenerative Medicine, Ssangyong-dong, Chungnam, Republic of Korea.
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Padalhin
authors.affiliation: Department of Regenerative Medicine, Ssangyong-dong, Chungnam, Republic of Korea.
authors.forename: Bo Ram
authors.initials: BR
authors.lastname: Kim
authors.affiliation: Department of Surgery, Soonchunhyang University, Cheonan Hospital, Cheonan, Republic of Korea.
authors.forename: Hae Il
authors.initials: HI
authors.lastname: Jung
authors.affiliation: Department of Regenerative Medicine, Ssangyong-dong, Chungnam, Republic of Korea Instititue of Tissue Regeneration, College of Medicine, Soonchunhyang University, Ssangyong-dong, Chungnam, Republic of Korea lbt@sch.ac.kr.
authors.forename: Byong-Taek
authors.initials: BT
authors.lastname: Lee
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001696:Biomechanical Phenomena; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes; D016503:Drug Delivery Systems; D005780:Gelatin; D006133:Growth Substances / Q000008:administration & dosage; D006801:Humans; D006820:Hyaluronic Acid; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006882:Hydroxyapatites; D008297:Male; D008422:Materials Testing; D051379:Mice; D053657:Platelet-Rich Plasma; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds
chemical_list: D018786:Bone Substitutes; D006133:Growth Substances; D006882:Hydroxyapatites; C074950:hydroxyapatite-beta tricalcium phosphate; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D005780:Gelatin; D006820:Hyaluronic Acid
keywords: Hyaluronic acid; biphasic calcium phosphate; bone substitutes; gelatin; platelet-rich plasma