title: Stromal myofibroblasts in squamous cell carcinoma of the tongue in young patients - a multicenter collaborative study.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the presence of myofibroblasts, frequently associated with a more aggressive neoplastic behavior, in oral tongue squamous cell carcinoma (TSCC) of young patients and to compare with the distribution observed in older patients.
  
  
  METHODS
  Tumor samples from 29 patients younger than 40 years old affected by TSCC were retrieved and investigated for the presence of stromal myofibroblasts by immunohistochemical reactions against α smooth muscle actin, and the results obtained were compared to TSCC cases affecting older patients.
  
  
  RESULTS
  No positive reaction could be found in the stromal areas devoid of neoplastic tissue, whereas myofibroblasts were present in 58.6% of the lesions in young patients and in 75.9% of the older ones. No significant difference was found when comparing the invasive front and the overall stroma of both groups, and no correlation could be obtained with stromal α smooth muscle actin expression, higher tumor grades or clinical stage (P > .05).
  
  
  CONCLUSIONS
  There was no significant difference between the presence of stromal myofibroblasts of TSCC affecting young and old individuals.
authors.affiliation: Department of Oral Diagnosis, University of Campinas (UNICAMP), Piracicaba Dental School, Piracicaba, Brazil.
authors.forename: Felipe Paiva
authors.initials: FP
authors.lastname: Fonseca
authors.affiliation: Department of Oral Diagnosis, University of Campinas (UNICAMP), Piracicaba Dental School, Piracicaba, Brazil.
authors.forename: Ricardo Della
authors.initials: RD
authors.lastname: Coletta
authors.affiliation: Department of Oral Diagnosis, University of Campinas (UNICAMP), Piracicaba Dental School, Piracicaba, Brazil.
authors.forename: Marina Barbosa
authors.initials: MB
authors.lastname: Azevedo
authors.affiliation: Service of Dental Oncology, Instituto do Cancer do Estado de São Paulo (ICESP), Faculdade de Medicina da Universidade de São Paulo (FMUSP), São Paulo, Brazil.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Prado Ribeiro
authors.affiliation: Department of Pathology and Clinical Propedeutic, Univ Estadual Paulista (UNESP), Araçatuba Dental School, Araçatuba, Brazil.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Pires Soubhia
authors.affiliation: Department of Pathology and Clinical Propedeutic, Univ Estadual Paulista (UNESP), Araçatuba Dental School, Araçatuba, Brazil.
authors.forename: Glauco Issamu
authors.initials: GI
authors.lastname: Miyahara
authors.affiliation: Centro Clinico de Cabeza y Cuello, Guatemala City, Guatemala.
authors.forename: Roman
authors.initials: R
authors.lastname: Carlos
authors.affiliation: Academic Unit of Oral and Maxillofacial Pathology, The University of Sheffield, School of Clinical Dentistry, Sheffield, UK.
authors.forename: Paula
authors.initials: P
authors.lastname: Farthing
authors.affiliation: Academic Unit of Oral and Maxillofacial Pathology, The University of Sheffield, School of Clinical Dentistry, Sheffield, UK.
authors.forename: Keith D
authors.initials: KD
authors.lastname: Hunter
authors.affiliation: Academic Unit of Oral and Maxillofacial Pathology, The University of Sheffield, School of Clinical Dentistry, Sheffield, UK.
authors.forename: Paul M
authors.initials: PM
authors.lastname: Speight
authors.affiliation: Department of Oral Diagnosis, University of Campinas (UNICAMP), Piracicaba Dental School, Piracicaba, Brazil.
authors.forename: Pablo Agustin
authors.initials: PA
authors.lastname: Vargas
authors.affiliation: Department of Oral Diagnosis, University of Campinas (UNICAMP), Piracicaba Dental School, Piracicaba, Brazil.
authors.forename: Oslei Paes
authors.initials: OP
authors.lastname: Almeida
authors.affiliation: Department of Oral Diagnosis, University of Campinas (UNICAMP), Piracicaba Dental School, Piracicaba, Brazil.
authors.forename: Marcio Ajudarte
authors.initials: MA
authors.lastname: Lopes
authors.affiliation: Department of Oral Diagnosis, University of Campinas (UNICAMP), Piracicaba Dental School, Piracicaba, Brazil. Electronic address: Alanroger@fop.unicamp.br.
authors.forename: Alan Roger
authors.initials: AR
authors.lastname: Santos-Silva
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D058628:Myofibroblasts / Q000473:pathology*; D012307:Risk Factors; D014062:Tongue Neoplasms / Q000473:pathology*