title: Artificial receptors for the extraction of nucleoside metabolite 7-methylguanosine from aqueous media made by molecular imprinting.
abstract: A series of imprinted polymers targeting nucleoside metabolites, prepared using a template analogue approach, are presented. These were prepared following selection of the optimum functional monomer by solution association studies using (1)H NMR titrations whereby methacrylic acid was shown to be the strongest receptor with and affinity constant of 621±51Lmol(-1)vs. 110±16Lmol(-1) for acrylamide. The best performing polymers were prepared using methanol as porogenic co-solvent and although average binding site affinities were marginally reduced, 2.3×10(4)Lmol(-1)vs. 2.7×10(4)Lmol(-1) measured for a polymer prepared in acetonitrile, these polymers contained the highest number of binding sites, 5.27μmolg(-1)vs. 1.64μmolg(-1), while they also exhibited enhanced selectivity for methylated guanosine derivatives. When applied as sorbents in the extraction of nucleoside derivative cancer biomarkers from synthetic urine samples, significant sample clean-up and recoveries of up to 90% for 7-methylguanosine were achieved.
authors.affiliation: University of Orleans, CNRS, I.C.O.A., UMR CNRS-7311, F-45067 Orléans, France.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Krstulja
authors.affiliation: University of Orleans, CNRS, I.C.O.A., UMR CNRS-7311, F-45067 Orléans, France.
authors.forename: Coralie
authors.initials: C
authors.lastname: De Schutter
authors.affiliation: University of Orleans, CNRS, I.C.O.A., UMR CNRS-7311, F-45067 Orléans, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Favetta
authors.affiliation: School of Chemistry and Chemical Engineering, Queen's University Belfast, David Keir Building, Stranmillis Road, BT9 5AG Belfast, N. Ireland, United Kingdom. Electronic address: p.manesiotis@qub.ac.uk.
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Manesiotis
authors.affiliation: University of Orleans, CNRS, I.C.O.A., UMR CNRS-7311, F-45067 Orléans, France. Electronic address: luigi.agrofoglio@univ-orleans.fr.
authors.forename: Luigi A
authors.initials: LA
authors.lastname: Agrofoglio
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D020106:Acrylamide / Q000737:chemistry; D000179:Acrylates / Q000737:chemistry; D000180:Acrylic Resins / Q000138:chemical synthesis / Q000737:chemistry*; D014408:Biomarkers, Tumor / Q000302:isolation & purification; D005240:Feasibility Studies; D006151:Guanosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D054802:Molecular Imprinting; D062165:Receptors, Artificial / Q000737:chemistry*; D052616:Solid Phase Extraction; D012996:Solutions; D012997:Solvents; D014867:Water / Q000737:chemistry*
chemical_list: D000097:Acetonitriles; D000179:Acrylates; D000180:Acrylic Resins; D014408:Biomarkers, Tumor; D008689:Methacrylates; D062165:Receptors, Artificial; D012996:Solutions; D012997:Solvents; D014867:Water; D006151:Guanosine; C008384:methacrylic acid; D020106:Acrylamide; C016578:7-methylguanosine; C031872:triacetylguanosine; C036658:acrylic acid; D000432:Methanol; C032159:acetonitrile
keywords: 7-Methyl guanosine; Bioanalysis; Molecular imprinting; Nucleosides; Solid phase extraction (SPE)