title: Microvascular quantification based on contour-scanning photoacoustic microscopy.
abstract: Accurate quantification of microvasculature remains of interest in fundamental pathophysiological studies and clinical trials. Current photoacoustic microscopy can noninvasively quantify properties of the microvasculature, including vessel density and diameter, with a high spatial resolution. However, the depth range of focus (i.e., focal zone) of optical-resolution photoacoustic microscopy (OR-PAM) is often insufficient to encompass the depth variations of features of interest—such as blood vessels—due to uneven tissue surfaces. Thus, time-consuming image acquisitions at multiple different focal planes are required to maintain the region of interest in the focal zone. We have developed continuous three-dimensional motorized contour-scanning OR-PAM, which enables real-time adjustment of the focal plane to track the vessels’ profile. We have experimentally demonstrated that contour scanning improves the signal-to-noise ratio of conventional OR-PAM by as much as 41% and shortens the image acquisition time by 3.2 times. Moreover, contour-scanning OR-PAM more accurately quantifies vessel density and diameter, and has been applied to studying tumors with uneven surfaces.
authors.affiliation: Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St. Louis, Missouri 63130, United States.
authors.forename: Chenghung
authors.initials: C
authors.lastname: Yeh
authors.affiliation: Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St. Louis, Missouri 63130, United States.
authors.forename: Brian
authors.initials: B
authors.lastname: Soetikno
authors.affiliation: Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St. Louis, Missouri 63130, United StatesbUniversity of Virginia, Department of Biomedical Engineering, PO Box 800759, Charlottesvill.
authors.forename: Song
authors.initials: S
authors.lastname: Hu
authors.affiliation: Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St. Louis, Missouri 63130, United States.
authors.forename: Konstantin I
authors.initials: KI
authors.lastname: Maslov
authors.affiliation: Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St. Louis, Missouri 63130, United States.
authors.forename: Lihong V
authors.initials: LV
authors.lastname: Wang
mesh_terms: D000818:Animals; D004423:Ear / Q000098:blood supply; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008546:Melanoma, Experimental / Q000098:blood supply; D051379:Mice; D008853:Microscopy / Q000379:methods*; D055806:Microvessels / Q000033:anatomy & histology*; D061088:Photoacoustic Techniques / Q000379:methods*; D059629:Signal-To-Noise Ratio; D012878:Skin Neoplasms / Q000098:blood supply