title: Atrial and ventricular specification of ADSCs is stimulated by different doses of BMP4.
abstract: To investigate the effect of BMP4 on cardiomyocyte differentiation of adipose tissue-derived stem cells (ADSCs), mouse ADSCs were treated with different concentrations of BMP4 in media containing fetal bovine serum (FBS) or Knockout™ Serum Replacement (KoSR). 3 weeks after cardiac induction, differentiated ADSCs expressed some cardiac-specific genes and proteins. BMP4 treatment upregulated the expression of cardiac transcription factors. In both FBS and KoSR-supplemented media, lower concentrations of BMP4 had a positive effect on the expression of MLC2A gene, while MLC2V was more expressed with higher concentrations of BMP4. BMP4 treatment in KoSR supplemented medium was more efficient for cardiac induction. Supplementation of culture media with insulin-transferrin-selenium improved the expression of MLC2A gene. The results of this study indicated that BMP4 is important for cardiac differentiation of the ADSCs. However, BMP4 was not enough for structural and functional maturation of the ADSC-derived cardiomyocytes.
authors.affiliation: Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box: 14965-161, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Khaleghi
authors.forename: Masoumeh Fakhr
authors.initials: MF
authors.lastname: Taha
authors.forename: Nazli
authors.initials: N
authors.lastname: Jafarzadeh
authors.forename: Arash
authors.initials: A
authors.lastname: Javeri
mesh_terms: D000818:Animals; D055415:Bone Morphogenetic Protein 4 / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D018994:Myosin Light Chains / Q000032:analysis / Q000235:genetics; D013234:Stem Cells / Q000187:drug effects* / Q000502:physiology*
chemical_list: C525809:BMP4 protein, human; D055415:Bone Morphogenetic Protein 4; D003470:Culture Media; C521958:Mylpf protein, mouse; D018994:Myosin Light Chains