title: First use of the WAVE™ disposable rocking bioreactor for enhanced bioproduct synthesis by N2 -fixing cyanobacteria.
abstract: WAVE™ rocking disposable bioreactors have been successfully utilized for bioproduct development from bacteria, yeast, microalgae, and animal and plant cells but not from cyanobacteria so far. N2 -fixing cyanobacteria represent a prolific bioproducts source with reduced cultivation costs. In this study, 1 L cultures of the N2 -fixing cyanobacterium Anabaena siamensis grown diazotrophically in the WAVE™ bioreactor exhibited increased phosphate consumption and 37-70% higher CO2 fixation rates than those grown in conventional bubbled suspension (BS) batch cultures. This generated 40-80% increased biomass productivities in the WAVE™ bioreactor reaching 60 mg L(-1)  day(-1) when supplemented with 10% CO2 . Consequently, WAVE™ generated 36-153% more protein, lipid, and carbohydrate than BS, including 47-100% increased productivity of phycocyanin and stearidonic acid (SA) with relevant biomedical applications. While the type of culture system (BS or WAVE(TM) ) did not affect the biochemical profile of cyanobacterial biomass, 10% CO2 supplementation induced a significant decrease in fatty acids and phycocyanin contents (mg g(-1)  DW). Therefore, for commercial applications, the CO2 supplementation of WAVE™ should be optimized for each targeted bioproduct separately. This study opens possibilities for upgrading the WAVE™ systems to photobioreactors (PBRs) for bioproduct development from cyanobacteria, with opportunities and challenges critically evaluated herein.
authors.affiliation: College of Marine and Environmental Sciences, James Cook University, Townsville, 4811, Queensland, Australia; Centre for Sustainable Fisheries and Aquaculture, James Cook University, Townsville, 4811, Queensland, Australia; Comparative Genomics Centre, James Cook University, Townsville, 4811, Queensland, Australia.
authors.forename: Samuel
authors.initials: S
authors.lastname: Cirés
authors.forename: Carlos
authors.initials: C
authors.lastname: Alvarez-Roa
authors.forename: Kirsten
authors.initials: K
authors.lastname: Heimann
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000295:instrumentation*; D003470:Culture Media; D000458:Cyanobacteria / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D015525:Fatty Acids, Omega-3 / Q000032:analysis / Q000378:metabolism; D058433:Photobioreactors / Q000382:microbiology*; D010798:Phycocyanin / Q000032:analysis / Q000378:metabolism
chemical_list: D003470:Culture Media; D015525:Fatty Acids, Omega-3; D010798:Phycocyanin; C062895:stearidonic acid
keywords: cyanobacteria; fatty acids; phycocyanin; rocking bioreactors; stearidonic acid; wave-mixed bioreactors