title: [RGD peptide-modified chitosan as a gene carrier of implant surface].
abstract: OBJECTIVE
  This study is conducted to explore new methods to perform surface biomodification of titanium implants and improve osteogenic efficiency.
  
  
  METHODS
  An RGD peptide and chitosan (CS) were combined by acylation reaction, forming RGD-CS. An RGD-CS/pDNA complex was subsequently prepared using a complex coacervation method and grafted on a pure titanium surface after physical and biochemical treatments were performed. The chemical structural characteristics of RGD-CS were evaluated using an infrared spectrometer and an elemental analyzer. The shape of this complex was then assessed by gel electrophoresis combined with atomic force microscopy. The grafting effect of this complex on the titanium surface was detected by EB staining.
  
  
  RESULTS
  CS and RGD peptides were coupled by an amide bond. The RGD-CS/pDNA complex was completely composited at N/P > or = 2. Atomic force microscopy results showed that the morphology of this complex was mainly spherical. EB staining experiments showed that this complex was successfully grafted on the titanium plate.
  
  
  CONCLUSIONS
  RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.
authors.forename: Di
authors.initials: D
authors.lastname: Zhang
authors.forename: Changhong
authors.initials: C
authors.lastname: Liu
authors.forename: Jincai
authors.initials: J
authors.lastname: Zhang
authors.forename: Dehong
authors.initials: D
authors.lastname: Cai
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Yang
authors.forename: Shiyi
authors.initials: S
authors.lastname: Li
authors.forename: Huilan
authors.initials: H
authors.lastname: Zhong
mesh_terms: D048271:Chitosan; D015921:Dental Implants; D018625:Microscopy, Atomic Force; D009842:Oligopeptides; D010957:Plasmids; D014025:Titanium
chemical_list: D015921:Dental Implants; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D048271:Chitosan; D014025:Titanium