title: Knockdown of ANLN by lentivirus inhibits cell growth and migration in human breast cancer.
abstract: Anillin (ANLN), an actin-binding protein, is required for cytokinesis. Recently, ANLN has been identified as a biomarker in diverse human cancers; however, the precise role of ANLN in breast cancer remains unclear. In this study, we firstly detected the expression of ANLN in 71 patients with breast cancer by immunohistochemistry, and found ANLN was highly expressed in breast cancer tissues. To evaluate the function of ANLN in breast cancer cells, we employed lentivirus-mediated RNA interference to knock down ANLN expression in two human breast cancer cell lines, MDA-MB-231, and ZR-75-30. Knockdown of ANLN remarkably inhibited the proliferation rate and colony formation ability of both breast cancer cell lines. Moreover, flow cytometry analysis showed that depletion of ANLN in MDA-MB-231 cells blocked the cell cycle progression, with more cells delayed at G2/M phase, due to phosphorylation of Cdc2 and suppression of Cyclin D1. Furthermore, knockdown of ANLN strongly suppressed the migration of breast cancer cells, strengthening the evidence that ANLN could be involved in breast cancer progression. Our results may suggest ANLN as a potential target candidate in breast cancer.
authors.affiliation: Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Weibing
authors.initials: W
authors.lastname: Zhou
authors.forename: Zhan
authors.initials: Z
authors.lastname: Wang
authors.forename: Ni
authors.initials: N
authors.lastname: Shen
authors.forename: Weiwei
authors.initials: W
authors.lastname: Pi
authors.forename: Wuzhong
authors.initials: W
authors.lastname: Jiang
authors.forename: Juan
authors.initials: J
authors.lastname: Huang
authors.forename: Yuanping
authors.initials: Y
authors.lastname: Hu
authors.forename: Xiong
authors.initials: X
authors.lastname: Li
authors.forename: Lunquan
authors.initials: L
authors.lastname: Sun
mesh_terms: D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D007150:Immunohistochemistry; D016086:Lentivirus / Q000235:genetics; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014410:Tumor Stem Cell Assay
chemical_list: C000591059:ANLN protein, human; D008840:Microfilament Proteins