title: Mode of action of allatostatins in the regulation of juvenile hormone biosynthesis in the cockroach, Diploptera punctata.
abstract: The FGLamide allatostatins (FGL/ASTs) are a family of neuropeptides with pleiotropic functions, including the inhibition of juvenile hormone (JH) biosynthesis, vitellogenesis and muscle contraction. In the cockroach, Diploptera punctata, thirteen FGLa/ASTs and one allatostatin receptor (AstR) have been identified. However, the mode of action of ASTs in regulation of JH biosynthesis remains unclear. Here, we determined the tissue distribution of Dippu-AstR. And we expressed Dippu-AstR in vertebrate cell lines, and activated the receptor with the Dippu-ASTs. Our results show that all thirteen ASTs activated Dippu-AstR in a dose dependent manner, albeit with different potencies. Functional analysis of AstR in multiple cell lines demonstrated that activation of the AstR receptor resulted in elevated levels of Ca(2+) and cAMP, which suggests that Dippu-AstR can act through the Gαq and Gαs protein pathways. The study on the target of AST action reveals that FGL/AST affects JH biosynthesis prior to the entry of acetyl-CoA into the JH biosynthetic pathway.
authors.affiliation: Department of Cell and Systems Biology, University of Toronto, Toronto, Canada.
authors.forename: Juan
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Cell and Systems Biology, University of Toronto, Toronto, Canada; Department of Biology, Zoological Institute, KU Leuven, B-3000 Leuven, Belgium.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Marchal
authors.affiliation: Department of Cell and Systems Biology, University of Toronto, Toronto, Canada.
authors.forename: Ekaterina F
authors.initials: EF
authors.lastname: Hult
authors.affiliation: Department of Biology, Zoological Institute, KU Leuven, B-3000 Leuven, Belgium.
authors.forename: Sven
authors.initials: S
authors.lastname: Zels
authors.affiliation: Department of Biology, Zoological Institute, KU Leuven, B-3000 Leuven, Belgium.
authors.forename: Jozef
authors.initials: J
authors.lastname: Vanden Broeck
authors.affiliation: Department of Cell and Systems Biology, University of Toronto, Toronto, Canada. Electronic address: stephen.tobe@utoronto.ca.
authors.forename: Stephen S
authors.initials: SS
authors.lastname: Tobe
mesh_terms: D000818:Animals; D002460:Cell Line; D003058:Cockroaches / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D006727:Hormone Antagonists; D006801:Humans; D019476:Insect Proteins / Q000378:metabolism*; D007605:Juvenile Hormones / Q000096:biosynthesis*; D008297:Male; D009479:Neuropeptides / Q000032:analysis / Q000378:metabolism*; D034622:RNA Interference; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D006727:Hormone Antagonists; D019476:Insect Proteins; D007605:Juvenile Hormones; D009479:Neuropeptides; C076523:allatostatin
keywords: Allatostatin receptor; Diploptera punctata; G protein-coupled receptors; Juvenile hormone; RNA interference