title: Immobilization of peroxidase enzyme onto the porous silicon structure for enhancing its activity and stability.
abstract: In this work, a commercial peroxidase was immobilized onto porous silicon (PS) support functionalized with 3-aminopropyldiethoxysilane (APDES) and the performance of the obtained catalytic microreactor was studied. The immobilization steps were monitored and the activity of the immobilized enzyme in the PS pores was spectrophotometrically determined. The enzyme immobilization in porous silicon has demonstrated its potential as highly efficient enzymatic reactor. The effect of a polar organic solvent (acetonitrile) and the temperature (up to 50°C) on the activity and stability of the biocatalytic microreactor were studied. After 2-h incubation in organic solvent, the microreactor retained 80% of its initial activity in contrast to the system with free soluble peroxidase that lost 95% of its activity in the same period of time. Peroxidase immobilized into the spaces of the porous silicon support would be perspective for applications in treatments for environmental security such as removal of leached dye in textile industry or in treatment of different industrial effluents. The system can be also applied in the field of biomedicine.
authors.affiliation: Centro de Investigacion en Ingenieria y Ciencias Aplicadas, UAEM, Av. Universidad 1001, Cuernavaca, Morelos 62209, México.
authors.forename: Padmavati
authors.initials: P
authors.lastname: Sahare
authors.affiliation: Instituto de Biotecnología Universidad Nacional Autónoma de México, Av. Universidad 2001, Cuernavaca, Morelos 62250, México.
authors.forename: Marcela
authors.initials: M
authors.lastname: Ayala
authors.affiliation: Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Km 107 Carretera Tijuana-Ensenada, Apdo Postal 14, CP. 22800 Ensenada, B.C., México.
authors.forename: Rafael
authors.initials: R
authors.lastname: Vazquez-Duhalt
authors.affiliation: Centro de Investigacion en Ingenieria y Ciencias Aplicadas, UAEM, Av. Universidad 1001, Cuernavaca, Morelos 62209, México.
authors.forename: Vivechana
authors.initials: V
authors.lastname: Agrawal
keywords: Immobilization; Microreactors; Peroxidase; Porous silicon