title: A ratiometric fluorescent probe based on a coumarin-hemicyanine scaffold for sensitive and selective detection of endogenous peroxynitrite.
abstract: In the study described herein, the red emitting probe CHCN, which possesses a linked coumarin-hemicyanine scaffold, was developed for detection of peroxynitrite (ONOO(-)) under physiological conditions. The studies show that CHCN displays a dual ratiometric and colorimetric response to ONOO(-) that is caused by an oxidation process. A possible mechanism of this oxidation process was proposed and confirmed by ESI-MS spectra for the first time. CHCN shown highly selective and sensitive towards ONOO(-) with a low limit of detection LOD (49.7 nM). Moreover, CHCN has appreciable cell permeability and, as a result, it is applicable to ratiometric detection of exogenous and endogenous ONOO(-) in living cells during phagocytic immune response. We anticipate that, owing to their ideal properties, probes of this type will find great use in explorations of the role played by ONOO(-) in biology.
authors.affiliation: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Republic of Korea; Department of Chemistry, Faculty of Science, Yanbian University, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Research Center for Human Natural Defense System, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea; BK 21 Plus Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea.
authors.forename: Younghee
authors.initials: Y
authors.lastname: Kwon
authors.affiliation: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Republic of Korea.
authors.forename: Gyoungmi
authors.initials: G
authors.lastname: Kim
authors.affiliation: Research Center for Human Natural Defense System, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea; BK 21 Plus Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea. Electronic address: yjh@yuhs.ac.
authors.forename: Ji-Hwan
authors.initials: JH
authors.lastname: Ryu
authors.affiliation: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Republic of Korea. Electronic address: jyoon@ewha.ac.kr.
authors.forename: Juyoung
authors.initials: J
authors.lastname: Yoon
mesh_terms: D015374:Biosensing Techniques; D003124:Colorimetry; D003374:Coumarins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D030421:Peroxynitrous Acid / Q000302:isolation & purification*; D013050:Spectrometry, Fluorescence
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; D030421:Peroxynitrous Acid; C030123:coumarin
keywords: Cell imaging; Fluorescent chemosensor; ONOO(−) sensor; Peroxynitrite sensor; Reactive oxygen species probe