title: Chikusetsusaponin V inhibits inflammatory responses via NF-κB and MAPK signaling pathways in LPS-induced RAW 264.7 macrophages.
abstract: Excessive activation of macrophages is implicated in various inflammation resulted injuries. Saponins from Panax japonicus (SPJ) have been shown to possess anti-inflammatory activities. However, whether Chikusetsusaponin V (CsV), the most abundant component of SPJ, can exert anti-inflammatory activities is unknown. The present study was aimed to investigate the anti-inflammatory effects of CsV in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells and the underlying mechanisms. Our data showed that CsV dose-dependently inhibited NO, iNOS, TNF-α and IL-1β expressions in LPS-stimulated RAW264.7 cells. Increased protein levels of nuclear NF-κB and elevated phosphorylation levels of ERK and JNK in LPS-stimulated RAW 264.7 cells were also found downregulated by CsV treatment. Furthermore, the increase of CD14 and TLR4 mRNA expression due to LPS stimulation were significantly reversed by CsV treatment. These results suggested that CsV attenuated LPS-induced inflammatory responses partly via TLR4/CD14-mediated NF-κB and MAPK pathways.
authors.affiliation: Department of Pharmacology .
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Dai
authors.forename: Yaoyan
authors.initials: Y
authors.lastname: Dun
authors.forename: Changcheng
authors.initials: C
authors.lastname: Zhang
authors.forename: Jingzhi
authors.initials: J
authors.lastname: Wan
authors.forename: Lili
authors.initials: L
authors.lastname: Deng
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zhou
authors.forename: Chaoqi
authors.initials: C
authors.lastname: Liu
authors.forename: Ding
authors.initials: D
authors.lastname: Yuan
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D015153:Blotting, Western; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D008070:Lipopolysaccharides / Q000494:pharmacology*; D020935:MAP Kinase Signaling System / Q000187:drug effects* / Q000276:immunology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D015394:Molecular Structure; D016328:NF-kappa B / Q000276:immunology*; D009569:Nitric Oxide / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D008070:Lipopolysaccharides; D016328:NF-kappa B; D012503:Saponins; C588229:chikusetsusaponin V; C482199:lipopolysaccharide, E coli O55-B5; D009569:Nitric Oxide
keywords: Chikusetsusaponin V; LPS; MAPK; NF-κBKW; inflammatory