title: JIP60-mediated, jasmonate- and senescence-induced molecular switch in translation toward stress and defense protein synthesis.
abstract: Two closely related genes encoding the jasmonate-induced protein 60 (JIP60) were identified in the barley genome. The gene on chromosome arm 4HL encodes the previously identified protein encoded by the cDNA X66376.1. This JIP60 protein is characterized here and shown to consist of two domains: an NH2-terminal domain related to ribosome-inactivating proteins and a COOH-terminal domain, which displays similarity to eukaryotic translation initiation factor 4E (eIF4E). JIP60 undergoes processing in vivo, as a result of which JIP60's COOH-terminal eIF4E domain is released and functions in recruiting a subset of cellular messengers for translation. This effect was observed for both MeJA-treated and naturally senescing plants. Because the JIP60 gene is in close proximity to several quantitative trait loci for both biotic and abiotic stress resistance, our results identify a unique target for future breeding programs.
authors.affiliation: Department of Crop and Soil Sciences, School of Molecular Biosciences, and Center for Reproductive Biology, Washington State University, Pullman, WA 99164-6420; rustgi@wsu.edu diter@wsu.edu sreinbot@ujf-grenoble.fr.
authors.forename: Sachin
authors.initials: S
authors.lastname: Rustgi
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, E-28223 Pozuelo de Alárcon (Madrid), Spain;
authors.forename: Stephan
authors.initials: S
authors.lastname: Pollmann
authors.affiliation: Lehrstuhl für Pflanzenphysiologie, Universität Bayreuth, D-95447 Bayreuth, Germany; and.
authors.forename: Frank
authors.initials: F
authors.lastname: Buhr
authors.affiliation: Lehrstuhl für Pflanzenphysiologie, Universität Bayreuth, D-95447 Bayreuth, Germany; and.
authors.forename: Armin
authors.initials: A
authors.lastname: Springer
authors.affiliation: Lehrstuhl für Pflanzenphysiologie, Universität Bayreuth, D-95447 Bayreuth, Germany; and.
authors.forename: Christiane
authors.initials: C
authors.lastname: Reinbothe
authors.affiliation: Department of Crop and Soil Sciences, School of Molecular Biosciences, and Center for Reproductive Biology, Washington State University, Pullman, WA 99164-6420; rustgi@wsu.edu diter@wsu.edu sreinbot@ujf-grenoble.fr.
authors.forename: Diter
authors.initials: D
authors.lastname: von Wettstein
authors.affiliation: Biologie Environnementale et Systémique, Université Joseph Fourier, BP53, F-38041 Grenoble Cedex 9, France rustgi@wsu.edu diter@wsu.edu sreinbot@ujf-grenoble.fr.
authors.forename: Steffen
authors.initials: S
authors.lastname: Reinbothe
mesh_terms: D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D003517:Cyclopentanes / Q000378:metabolism / Q000494:pharmacology; D039561:Eukaryotic Initiation Factor-4E / Q000378:metabolism; D020869:Gene Expression Profiling; D017343:Genes, Plant; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D001467:Hordeum / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000378:metabolism / Q000494:pharmacology; D010802:Phylogeny; D010940:Plant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D040641:Quantitative Trait Loci; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
chemical_list: D003517:Cyclopentanes; D039561:Eukaryotic Initiation Factor-4E; D006360:Heat-Shock Proteins; C091875:JIP60 protein, Hordeum vulgare; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid
keywords: Hordeum vulgare; jasmonic acid; translational reprogramming