title: Cordyceps militaris extract protects human dermal fibroblasts against oxidative stress-induced apoptosis and premature senescence.
abstract: Oxidative stress induced by reactive oxygen species (ROS) is the major cause of degenerative disorders including aging and disease. In this study, we investigated whether Cordyceps militaris extract (CME) has in vitro protective effects on hydrogen peroxide-induced oxidative stress in human dermal fibroblasts (HDFs). Our results showed that the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of CME was increased in a dose-dependent manner. We found that hydrogen peroxide treatment in HDFs increased ROS generation and cell death as compared with the control. However, CME improved the survival of HDFs against hydrogen peroxide-induced oxidative stress via inhibition of intracellular ROS production. CME treatment inhibited hydrogen peroxide-induced apoptotic cell death and apoptotic nuclear condensation in HDFs. In addition, CME prevented hydrogen peroxide-induced SA-β-gal-positive cells suggesting CME could inhibit oxidative stress-induced premature senescence. Therefore, these results suggest that CME might have protective effects against oxidative stress-induced premature senescence via scavenging ROS.
authors.affiliation: Department of Bioengineering and Technology, Kangwon National University, Chuncheon 200701, Korea. three0313@nate.com.
authors.forename: Jun Myoung
authors.initials: JM
authors.lastname: Park
authors.affiliation: Department of Bioengineering and Technology, Kangwon National University, Chuncheon 200701, Korea. jongseoklee78@gmail.com.
authors.forename: Jong Seok
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Bioengineering and Technology, Kangwon National University, Chuncheon 200701, Korea. lkr3410@nate.com.
authors.forename: Ki Rim
authors.initials: KR
authors.lastname: Lee
authors.affiliation: Department of Bioengineering and Technology, Kangwon National University, Chuncheon 200701, Korea. sjha@kangwon.ac.kr.
authors.forename: Suk-Jin
authors.initials: SJ
authors.lastname: Ha
authors.affiliation: Department of Bioengineering and Technology, Kangwon National University, Chuncheon 200701, Korea. ekhong@kangwon.ac.kr.
authors.forename: Eock Kee
authors.initials: EK
authors.lastname: Hong
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001688:Biological Products / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D032661:Cordyceps; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide; D018384:Oxidative Stress / Q000187:drug effects*; D010851:Picrates / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001688:Biological Products; D001713:Biphenyl Compounds; D010851:Picrates; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl