title: Epidermal growth factor receptor expression in canine transitional cell carcinoma.
abstract: Transitional cell carcinoma (TCC), a urinary bladder tumor with high mortality, is encountered commonly in dogs. Whereas overexpression of epidermal growth factor receptor (EGFR) is associated with development of human urinary bladder cancer, information on EGFR expression in canine TCC is lacking. In this study, EGFR protein and mRNA expression in canine normal bladder (n=5), polypoid cystitis (n=5) and TCC (n=25) were examined by immunohistochemistry and real-time polymerase chain reaction. EGFR protein expression was significantly higher in TCC than that in normal healthy bladder (P<0.001) and polypoid cystitis (P<0.005). High EGFR protein expression was significantly (P<0.01) associated with TCC with a sensitivity of 72% and specificity of 100%. Comparative analysis of protein and mRNA expression levels in TCC showed significant positive correlation (r=0.88, P<0.05) between mRNA and protein expression. These findings suggest that intense expression of EGFR protein could be used as a marker to help canine TCC diagnosis.
authors.affiliation: Department of Small Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai Midorimachi, Ebetsu-shi, Hokkaido 069-8501, Japan.
authors.forename: Kiwamu
authors.initials: K
authors.lastname: Hanazono
authors.forename: Shinya
authors.initials: S
authors.lastname: Fukumoto
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kawamura
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Endo
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kadosawa
authors.forename: Hidetomo
authors.initials: H
authors.lastname: Iwano
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Uchide
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor; D002295:Carcinoma, Transitional Cell / Q000378:metabolism / Q000662:veterinary*; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000662:veterinary*
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D066246:ErbB Receptors