title: Wide-field, high-resolution Fourier ptychographic microscopy.
abstract: In this article, we report an imaging method, termed Fourier ptychographic microscopy (FPM), which iteratively stitches together a number of variably illuminated, low-resolution intensity images in Fourier space to produce a wide-field, high-resolution complex sample image. By adopting a wavefront correction strategy, the FPM method can also correct for aberrations and digitally extend a microscope's depth-of-focus beyond the physical limitations of its optics. As a demonstration, we built a microscope prototype with a resolution of 0.78 μm, a field-of-view of ~120 mm2, and a resolution-invariant depth-of-focus of 0.3 mm (characterized at 632 nm). Gigapixel colour images of histology slides verify FPM's successful operation. The reported imaging procedure transforms the general challenge of high-throughput, high-resolution microscopy from one that is coupled to the physical limitations of the system's optics to one that is solvable through computation.
authors.affiliation: Electrical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: Guoan
authors.initials: G
authors.lastname: Zheng
authors.affiliation: Electrical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: Roarke
authors.initials: R
authors.lastname: Horstmeyer
authors.affiliation: Electrical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: Changhuei
authors.initials: C
authors.lastname: Yang
keywords: digital pathology; gigapixel microscopy; high-throughput imaging; phase retrieval