title: Expression of chemokines and chemokine receptors in brain tumor tissue derived cells.
abstract: Chemokine and chemokine receptor expression by tumor cells contributes to tumor growth and angiogenesis and thus these factors may be considered as tumor markers. Here we aimed to characterize cells directly extracted from glioma, meningioma, and secondary brain tumors as well as non-tumoral cells in vitro. Cells were isolated from brain tissues using 0.2% collagenase and characterized by flow cytometry. Expression of SDF-1, CXCR4, CXCR7, RANTES, CCR5, MCP-1 and IP-10 was defined using flow cytometry and qRT-PCR methods. Brain tissue isolated cells were observed as spindle-shaped cell populations. No significant differences were observed for expression of SDF-1, CXCR4, CXCR7, RANTES, CCR5, and IP-10 transcripts. However, the expression of CXCR4 was approximately 13-fold and 110-fold higher than its counterpart, CXCR7, in meningioma and glioma cells, respectively. CXCR7 was not detectable in secondary tumors but CXCR4 was expressed. In non tumoral cells, CXCR7 had 1.3-fold higher mRNA expression than CXCR4. Flow cytometry analyses of RANTES, MCP- 1, IP-10, CCR5 and CXCR4 expression showed no significant difference between low and high grade gliomas. Differential expression of CXCR4 and CXCR7 in brain tumors derived cells compared to non-tumoral samples may have crucial impacts on therapeutic interventions targeting the SDF-1/CXCR4/CXCR7 axis.
authors.affiliation: Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran E-mail : ghaderia@sums.ac.ir.
authors.forename: Mahboobeh
authors.initials: M
authors.lastname: Razmkhah
authors.forename: Fahimeh
authors.initials: F
authors.lastname: Arabpour
authors.forename: Mousa
authors.initials: M
authors.lastname: Taghipour
authors.forename: Ali
authors.initials: A
authors.lastname: Mehrafshan
authors.forename: Nooshafarin
authors.initials: N
authors.lastname: Chenari
authors.forename: Abbas
authors.initials: A
authors.lastname: Ghaderi
mesh_terms: D000328:Adult; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000556:secondary; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018932:Chemokine CCL2; D018946:Chemokine CCL5; D054377:Chemokine CXCL12; D018925:Chemokines / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008579:Meningioma / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism*; D019713:Receptors, CCR5; D054387:Receptors, CXCR; D019718:Receptors, CXCR4; D019707:Receptors, Chemokine / Q000235:genetics* / Q000378:metabolism; D018121:Receptors, Cytokine; D012509:Sarcoma / Q000235:genetics* / Q000378:metabolism / Q000556:secondary; D014407:Tumor Cells, Cultured
chemical_list: C501896:ACKR3 protein, human; C111835:CCL2 protein, human; C483024:CCL5 protein, human; C584933:CCR5 protein, human; C515255:CXCL12 protein, human; C527276:CXCR4 protein, human; D018932:Chemokine CCL2; D018946:Chemokine CCL5; D054377:Chemokine CXCL12; D018925:Chemokines; C104577:IP10-Mig receptor; D012333:RNA, Messenger; D019713:Receptors, CCR5; D054387:Receptors, CXCR; D019718:Receptors, CXCR4; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine