title: Rare allele of HvLox-1 associated with lipoxygenase activity in barley (Hordeum vulgare L.).
abstract: Identification and allele-specific marker development of a functional SNP of HvLox - 1 which associated with barley lipoxygenase activity. Improving the stability of the flavor of beer is one of the main objectives in breeding barley for malting, and lipoxygenase-1 (LOX-1) is a key enzyme controlling this trait. In this study, a modified LOX activity assay was used for null LOX-1 mutant screening. Four barley landraces with no detected level of LOX-1 activity were screened from 1,083 barley germplasm accessions from China. The genomic sequence diversity of the HvLox-1 gene of the four null LOX-1 Chinese landraces was compared with that of a further 76 accessions. A total of 104 nucleotide polymorphisms were found, which contained 83 single-nucleotide polymorphisms (SNPs), 7 multiple-nucleotide polymorphisms, and 14 insertions and deletions. Most notably, we found a rare C/G mutation (SNP-61) in the second intron which led to null LOX-1 activity through an altered splicing acceptor site. In addition, an allele-specific polymerase chain reaction marker was developed for the genotyping of SNP-61, which could be used in breeding programs for barley to be used for malting. The objective was to improve beer quality.
authors.affiliation: Key Laboratory of Crop Germplasm Resources and Utilization (Ministry of Agriculture), The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Ganggang
authors.initials: G
authors.lastname: Guo
authors.forename: Dawa
authors.initials: D
authors.lastname: Dondup
authors.forename: Xingmiao
authors.initials: X
authors.lastname: Yuan
authors.forename: Fanghong
authors.initials: F
authors.lastname: Gu
authors.forename: Deliang
authors.initials: D
authors.lastname: Wang
authors.forename: Fengchao
authors.initials: F
authors.lastname: Jia
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Lin
authors.forename: Michael
authors.initials: M
authors.lastname: Baum
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000483:Alleles; D001947:Breeding; D002681:China; D002874:Chromosome Mapping; D005838:Genotype; D001467:Hordeum / Q000201:enzymology / Q000235:genetics*; D007438:Introns; D008084:Lipoxygenase / Q000235:genetics / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide
chemical_list: D008084:Lipoxygenase