title: A proteomics study of hyperhomocysteinemia injury of the hippocampal neurons using iTRAQ.
abstract: High levels of homocysteine, caused by abnormal methionine metabolism, can induce degeneration of mouse hippocampal neurons. iTRAQ™ technology has been widely used in the field of proteomics research and through employing this technology, the present study identified that hyperhomocysteinemia induced the downregulation of 52 proteins and upregulation of 44 proteins in the mouse hippocampus. Through gene ontology and pathway analysis, the upregulation of components of the cytoskeleton, actin, regulators of focal adhesion, calcium signaling pathways, tight junctions, ErbB and gonadotrophin‑releasing hormone signaling, leukocyte, transendothelial migration, propanoate and pyruvate metabolism, valine, leucine and isoleucine biosynthesis, synthesis and degradation of ketone bodies and benzoate degradation via CoA ligation pathway, was identified. It was additionally verified that tau protein was highly expressed in the hyperhomocysteinemic neurons. Further analysis revealed that tau network proteins played functional roles in homocysteine‑induced neuronal damage.
authors.affiliation: Department of Neurology, Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Fang
authors.affiliation: Department of Neurology, Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurology, Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, P.R. China.
authors.forename: Han
authors.initials: H
authors.lastname: Yan
authors.affiliation: Department of Neurology, Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, P.R. China.
authors.forename: Yan-Xin
authors.initials: YX
authors.lastname: Zhao
authors.affiliation: Department of Neurology, Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, P.R. China.
authors.forename: Xue-Yuan
authors.initials: XY
authors.lastname: Liu
mesh_terms: D000818:Animals; D063990:Gene Ontology; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology*; D020138:Hyperhomocysteinemia / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism*; D060066:Protein Interaction Maps; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D015398:Signal Transduction
chemical_list: D020543:Proteome