title: A novel parainfluenza virus type 3 (PIV3) identified from goat herds with respiratory diseases in eastern China.
abstract: Parainfluenza virus type 3 (PIV3) is one of the most important viral respiratory pathogens for humans and for many animals, but goat infection has been rarely reported. Starting in Aug 2013, goats in the Jiangsu and Anhui provinces of eastern China suffered severe respiratory diseases. In order to identify the causative agent, numerous related pathogens were tested with RT-PCR or PCR. A unique PIV3 strain was detected in most of the clinical nasal swabs or serum samples. The virus was isolated on MDBK cells and characterized by RT-PCR, nucleotide sequence analysis and hemagglutination test. The entire M and F gene coding regions, HN, 5'-UTR-N and L gene fragments were amplified using pairs of degenerate primers. Nucleotide, amino acid sequence alignments and phylogenetic analyses based on these genes indicated that the goat-derived PIV3 strain was distinct from previously reported BPIV3 genotypes and HPIV3 strains. The novel isolate, named JS2013, might be a potentially new member of the respirovirus genus. Goats were experimentally infected with JS2013 culture. The virus-inoculated goats displayed coughing and nasal discharges that were related to respiratory diseases. Viremia and virus shedding were detected during 4-10 days post-inoculation (dpi). Virus-specific HI antibodies became positive from 14 dpi. This is the first report of the detection of PIV3 from Chinese goat herds and genetic and pathogenetic characterization of the novel goat-derived PIV3.
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
authors.forename: Wenliang
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
authors.forename: Li
authors.initials: L
authors.lastname: Mao
authors.affiliation: Hai'an Animal Husbandry and Veterinary Station, Hai'an 226600, China.
authors.forename: Suping
authors.initials: S
authors.lastname: Cheng
authors.affiliation: Hai'an Animal Husbandry and Veterinary Station, Hai'an 226600, China.
authors.forename: Qiusheng
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Dianshi Biological Technology Co., Ltd., Nantong 226000, China.
authors.forename: Jiachun
authors.initials: J
authors.lastname: Huang
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China; College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
authors.forename: Jiawu
authors.initials: J
authors.lastname: Deng
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Zhongyu
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
authors.forename: Leilei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Hao
authors.affiliation: Hai'an Animal Husbandry and Veterinary Station, Hai'an 226600, China.
authors.forename: Yonglong
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Hai'an Animal Husbandry and Veterinary Station, Hai'an 226600, China.
authors.forename: Yinhua
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Wei
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. Electronic address: jieyuanj57@gmail.com.
authors.forename: Jieyuan
authors.initials: J
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D015511:Goat Diseases / Q000453:epidemiology* / Q000821:virology*; D006041:Goats; D006386:Hemagglutination Tests / Q000662:veterinary; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D018108:Respirovirus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D010253:Respirovirus Infections / Q000453:epidemiology / Q000662:veterinary*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013045:Species Specificity
chemical_list: D017931:DNA Primers
keywords: Goat; PIV3; Pathogenicity; Phylogenetic analysis; Respiratory disease