title: [Effects of acupuncture intervention on expression of glucose-regulated protein 78 and C/EBP homologous protein in hippocampal CA 1 region in rats with hyperspasmia].
abstract: OBJECTIVE
  To observe the effect of acupuncture intervention on expression of glucose-regulated protein 78 (Grp 78) and C/EBP homologous protein (CHOP) in the hippocampus in epilepsy rats so as to explore its mechanism underlying improvement of hyperspasmia-induced brain injury.
  
  
  METHODS
  Forty-two SD rats were randomly divided into normal control group (n = 6), model group (n = 18), and acupuncture group (n = 18). The epileptic seizure model was established by intraperitonel injection of Pentylenetetrazol (50 mg/kg, 2 mL). Manual acupuncture stimulation of "Baihui" (GV 20) and "Dazhui" (GV 14) was conducted for rats of the acupuncture group for 30 min. Two hours (h), 12 h and 48 h after acupuncture intervention, the hippocampal tissue was sampled (6 rats at each time-point). The expression levels of Grp 78 and CHOP proteins in the hippocampal CA 1 region were detected by immunohistochemistry.
  
  
  RESULTS
  Compared with the normal group, the expression levels of Grp 78 protein at time-points of 2 h and 12 h, and those of CHOP protein at 2 h, 12 h and 48 h after epilpeptic seizure were significantly increased in the model group (P < 0.01). After acupuncture treatment, the expression levels of Grp 78 at 12 and 48 h were significantly increased, and those of CHOP protein at 2 h, 12 h and 24 h in the acupuncture group were considerably downregulated (P < 0.05, P < 0.01).
  
  
  CONCLUSIONS
  Acupuncture treatment can up-regulate Grp 78 protein expression and down-regulate CHOP protein expression level in epilepsy rats , which may contribute to its protective effect on seizure-induced brain injury.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Yun
authors.initials: Y
authors.lastname: Ma
authors.forename: Wen-Ping
authors.initials: WP
authors.lastname: Ang
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Du
authors.forename: Sheng-Bing
authors.initials: SB
authors.lastname: Wu
authors.forename: Lei
authors.initials: L
authors.lastname: Lü
authors.forename: Dao-Qin
authors.initials: DQ
authors.lastname: Zhang
mesh_terms: D015670:Acupuncture Therapy; D000818:Animals; D056547:CA1 Region, Hippocampal / Q000378:metabolism*; D004195:Disease Models, Animal; D004827:Epilepsy / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D005260:Female; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D051381:Rats; D051742:Transcription Factor CHOP / Q000235:genetics / Q000378:metabolism; D000091342:Endoplasmic Reticulum Chaperone BiP
chemical_list: D006360:Heat-Shock Proteins; D051742:Transcription Factor CHOP; C051684:GRP78 protein, rat; D000091342:Endoplasmic Reticulum Chaperone BiP