title: Transcript and metabolite alterations increase ganoderic acid content in Ganoderma lucidum using acetic acid as an inducer.
abstract: Acetic acid at 5-8 mM increased ganoderic acid (GA) accumulation in Ganoderma lucidum. After optimization by the response surface methodology, the GA content reached 5.5/100 mg dry weight, an increase of 105% compared with the control. The intermediate metabolites of GA biosynthesis, lanosterol and squalene also increased to 47 and 15.8 μg/g dry weight, respectively, in response to acetic acid. Acetic acid significantly induced transcription levels of sqs, lano, hmgs and cyp51 in the GA biosynthesis pathway. An acetic acid-unregulated acetyl coenzyme A synthase (acs) gene was selected from ten candidate homologous acs genes. The results indicate that acetic acid alters the expression of genes related to acetic acid assimilation and increases GA biosynthesis and the metabolic levels of lanosterol, squalene and GA-a, thereby resulting in GA accumulation.
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China.
authors.forename: Ang
authors.initials: A
authors.lastname: Ren
authors.forename: Xiong-Biao
authors.initials: XB
authors.lastname: Li
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Miao
authors.forename: Liang
authors.initials: L
authors.lastname: Shi
authors.forename: Ai-Liang
authors.initials: AL
authors.lastname: Jaing
authors.forename: Ming-Wen
authors.initials: MW
authors.lastname: Zhao
mesh_terms: D019342:Acetic Acid / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000187:drug effects; D007810:Lanosterol / Q000032:analysis; D020940:Reishi / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013185:Squalene / Q000032:analysis; D014158:Transcription, Genetic / Q000187:drug effects; D014315:Triterpenes / Q000032:analysis*
chemical_list: D014315:Triterpenes; C556862:ganoderic acid; D007810:Lanosterol; D013185:Squalene; D019342:Acetic Acid