title: Effect of finishing period length with α-tocopherol supplementation on the expression of vitamin E-related genes in the muscle and subcutaneous fat of light lambs.
abstract: The aim of this study was to investigate how different finishing period lengths with α-tocopherol supplementation or alfalfa grazing affect mRNA expression levels of genes related to vitamin E metabolism in L. thoracis (LT) muscle and subcutaneous fat (SF) from lambs of the Rasa Aragonesa breed. Indoors, concentrate-fed light lambs (n=48) were supplemented with 500 dl-α-tocopheryl acetate/kg concentrate for an average finishing period length of 0 (C), 10.7 (VE10d), 21.2 (VE20d) and, 32.3 (VE30d) days before slaughtering. Simultaneously, 8 lambs with their dams were alfalfa-grazed. The α-tocopherol affected in a short-term the expression of genes in LT muscle (ABCA1, LPL, APOE, and SREBP1) and SF (ABCA1, SCARB1, LPL, and PPARG). On the contrary, PPARA gene expression showed a long-term α-tocopherol effect because the highest levels of PPARA mRNA were found in the VE30d.
authors.affiliation: Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Unidad de Tecnología en Producción Animal, Avda. Montañana, 930, 50059 Zaragoza, Spain.
authors.forename: L
authors.initials: L
authors.lastname: González-Calvo
authors.affiliation: Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Unidad de Tecnología en Producción Animal, Avda. Montañana, 930, 50059 Zaragoza, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Joy
authors.affiliation: Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Unidad de Tecnología en Producción Animal, Avda. Montañana, 930, 50059 Zaragoza, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Alberti
authors.affiliation: Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Unidad de Tecnología en Producción Animal, Avda. Montañana, 930, 50059 Zaragoza, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Ripoll
authors.affiliation: Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Unidad de Tecnología en Producción Animal, Avda. Montañana, 930, 50059 Zaragoza, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Molino
authors.affiliation: Departamento de Mejora Genética animal, INIA, 28040 Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Serrano
authors.affiliation: Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Unidad de Tecnología en Producción Animal, Avda. Montañana, 930, 50059 Zaragoza, Spain; ARAID, C/ María de Luna, n° 11, 1ª planta, Edificio CEEI Aragón, 50018 Zaragoza, Spain. Electronic address: jhcalvo@aragon.es.
authors.forename: J H
authors.initials: JH
authors.lastname: Calvo
mesh_terms: D000821:Animal Feed; D000818:Animals; D004032:Diet; D005260:Female; D015870:Gene Expression; D008297:Male; D008460:Meat; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D012756:Sheep / Q000502:physiology; D024502:alpha-Tocopherol / Q000008:administration & dosage*
chemical_list: D024502:alpha-Tocopherol
keywords: Feeding system; Gene expression; Sheep; Vitamin E