title: mRNA expression and localization of LPS-induced β-defensin isoforms in rat salivary glands.
abstract: β-defensins are small, cationic peptides with broad-spectrum antimicrobial activity that are produced by mucosal epithelia. However, little is known about the expression of β-defensins in the major salivary glands. The purpose of this study was to characterize expression of rat β-defensin-1 (RBD-1) and -2 (RBD-2) mRNA within the major salivary glands together with the effect of injection of intraductal lipopolysaccharide (LPS) on that expression. β-defensin mRNA expression was quantitated by RT-PCR in salivary gland tissues and salivary acinar and striated duct cells collected by laser captured microdissection. RBD-1 and -2 were expressed in the parotid gland, the submandibular gland, and the sublingual gland. β-defensins were expressed in both the acinar and striated duct cells of the major salivary glands. Intraductal injection of LPS increased expression of RBD-1 and -2 mRNA, which peaked at 12 hrs. These results suggest that salivary cells (acinar and striated duct cells) have the potential to produce β-defensins.
authors.affiliation: Department of Pharmacology, Tokyo Dental College.
authors.forename: Takashi
authors.initials: T
authors.lastname: Shinomiya
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Kawaguchi
authors.forename: Migiwa
authors.initials: M
authors.lastname: Okubo
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kosuge
authors.forename: Masanobu
authors.initials: M
authors.lastname: Yoshikawa
mesh_terms: D000818:Animals; D023082:Defensins / Q000032:analysis / Q000187:drug effects; D004926:Escherichia coli; D017403:In Situ Hybridization; D053685:Laser Therapy / Q000379:methods; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D042282:Microdissection / Q000379:methods; D010306:Parotid Gland / Q000737:chemistry / Q000187:drug effects; D020033:Protein Isoforms / Q000032:analysis / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis* / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018987:Salivary Ducts / Q000737:chemistry / Q000187:drug effects; D012469:Salivary Glands / Q000737:chemistry* / Q000187:drug effects; D013361:Sublingual Gland / Q000737:chemistry / Q000187:drug effects; D013363:Submandibular Gland / Q000737:chemistry / Q000187:drug effects; D013997:Time Factors; D023083:beta-Defensins / Q000032:analysis* / Q000187:drug effects
chemical_list: C478968:Defb1 protein, rat; D023082:Defensins; D008070:Lipopolysaccharides; D020033:Protein Isoforms; D012333:RNA, Messenger; D023083:beta-Defensins; C549474:beta-defensin-2, rat