title: Effects of acute doxorubicin treatment on hepatic proteome lysine acetylation status and the apoptotic environment.
abstract: OBJECTIVE
  To determine if doxorubicin (Dox) alters hepatic proteome acetylation status and if acetylation status was associated with an apoptotic environment.
  
  
  METHODS
  Doxorubicin (20 mg/kg; Sigma, Saint Louis, MO; n = 8) or NaCl (0.9%; n = 7) was administered as an intraperitoneal injection to male F344 rats, 6-wk of age. Once animals were treated with Dox or saline, all animals were fasted until sacrifice 24 h later.
  
  
  RESULTS
  Dox treatment decreased proteome lysine acetylation likely due to a decrease in histone acetyltransferase activity. Proteome deacetylation may likely not be associated with a proapoptotic environment. Dox did not increase caspase-9, -8, or -3 activation nor poly (adenosine diphosphate-ribose) polymerase-1 cleavage. Dox did stimulate caspase-12 activation, however, it likely did not play a role in apoptosis induction.
  
  
  CONCLUSIONS
  Early effects of Dox involve hepatic proteome lysine deacetylation and caspase-12 activation under these experimental conditions.
authors.affiliation: Amie J Dirks-Naylor, Samir A Kouzi, Joseph D Bero, Ngan TK Tran, Sendra Yang, Raean Mabolo, School of Pharmacy, Wingate University, Wingate, NC 28174, United States.
authors.forename: Amie J
authors.initials: AJ
authors.lastname: Dirks-Naylor
authors.affiliation: Amie J Dirks-Naylor, Samir A Kouzi, Joseph D Bero, Ngan TK Tran, Sendra Yang, Raean Mabolo, School of Pharmacy, Wingate University, Wingate, NC 28174, United States.
authors.forename: Samir A
authors.initials: SA
authors.lastname: Kouzi
authors.affiliation: Amie J Dirks-Naylor, Samir A Kouzi, Joseph D Bero, Ngan TK Tran, Sendra Yang, Raean Mabolo, School of Pharmacy, Wingate University, Wingate, NC 28174, United States.
authors.forename: Joseph D
authors.initials: JD
authors.lastname: Bero
authors.affiliation: Amie J Dirks-Naylor, Samir A Kouzi, Joseph D Bero, Ngan TK Tran, Sendra Yang, Raean Mabolo, School of Pharmacy, Wingate University, Wingate, NC 28174, United States.
authors.forename: Ngan Tk
authors.initials: NT
authors.lastname: Tran
authors.affiliation: Amie J Dirks-Naylor, Samir A Kouzi, Joseph D Bero, Ngan TK Tran, Sendra Yang, Raean Mabolo, School of Pharmacy, Wingate University, Wingate, NC 28174, United States.
authors.forename: Sendra
authors.initials: S
authors.lastname: Yang
authors.affiliation: Amie J Dirks-Naylor, Samir A Kouzi, Joseph D Bero, Ngan TK Tran, Sendra Yang, Raean Mabolo, School of Pharmacy, Wingate University, Wingate, NC 28174, United States.
authors.forename: Raean
authors.initials: R
authors.lastname: Mabolo
keywords: Acetylation; Apoptosis; Caspase; Histone acetyltransferase; Histone deacetylase; Sirtuin 1; Sirtuin 3