title: Improved isolation protocol to detect high molecular weight polysaccharide structures of Campylobacter jejuni.
abstract: Simple detection of high molecular weight, LPS-like structures of Campylobacter jejuni is still an unsolved problem. A phenol-free extraction method for the detection of HMW polysaccharide was developed without the need for Western blot. This method provides a reliable technique for large-scale screening and comparative characterization study of different isolates.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Pécs, Hungary. Electronic address: fedorita85@gmail.com.
authors.forename: Judit K
authors.initials: JK
authors.lastname: Kovács
authors.affiliation: Department of Medical Microbiology and Immunology, University of Pécs, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Felső
authors.affiliation: Department of Medical Microbiology and Immunology, University of Pécs, Hungary; Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Levente
authors.initials: L
authors.lastname: Emődy
authors.affiliation: Department of Medical Microbiology and Immunology, University of Pécs, Hungary.
authors.forename: György
authors.initials: G
authors.lastname: Schneider
authors.affiliation: Department of Medical Microbiology and Immunology, University of Pécs, Hungary.
authors.forename: Béla
authors.initials: B
authors.lastname: Kocsis
mesh_terms: D016123:Campylobacter jejuni / Q000737:chemistry*; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D011135:Polysaccharides, Bacterial
keywords: Campylobacter jejuni; LPS; Polysaccharide; Silver staining