title: [Regulating effect of anodonta glucan HBP-A on chondrocytes through Wnt pathway].
abstract: OBJECTIVE
  To investigate regulation function of anodonta glucan HBP-A on chondrocytes through Wnt pathway in vitro.
  
  
  METHODS
  Rat chondrocytes were cultured and differentiated induced with IL-1beta (10 ng/ml) in vitro. Chondrocytes were divided into five groups:IL-13 group,IL-1beta + IWP-2 (5 microM,Wnt pathway inhibitor) group, IL-1beta + HBP-A (0.3 mg/ml) group and IL-1beta + IWP-2 + HBP-A group. Wnt-3a, beta-catenin (24 h,48 h,72 h) and MMP-13(72 h) genes expression were detected by Rt-PCR, while beta-catenin, MMP-13, Sox-9 and coll-II (48 h) protein expression were measured by Western-blot.
  
  
  RESULTS
  After induction of IL-1beta, gene expression of Wnt-3a, beta-catenin and MMP-13 were increased,so were the protein expression of beta-catenin and MMP-13. In contrast,protein expression of Sox-9 and Coll-II were declined. Following addition of HBP-A, Wnt-3a, beta-catenin and MMP-13 were shown as induction of IL-1beta, but protein expression of Sox-9 and Coll-II were upgraded. Combining HBP-A with IWP-2 led to the lowest level in Wnt-3a, beta-catenin gene and beta-catenin protein expression and highest expression of Sox-9 protein.
  
  
  CONCLUSIONS
  HBP-A could not only delay the differentiation of chondrocytes through downgrading the signal expression of Wnt/beta-catenin,but also adjust the expression of Wnt-3a, beta-catenin and Sox-9 when combinated with the Wnt inhibitor.
authors.forename: Song-Pu
authors.initials: SP
authors.lastname: Wei
authors.forename: Dao-Fang
authors.initials: DF
authors.lastname: Ding
authors.forename: Xue-Zong
authors.initials: XZ
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Pang
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Zheng
authors.forename: Qin-Guang
authors.initials: QG
authors.lastname: Xu
authors.forename: Yue-Long
authors.initials: YL
authors.lastname: Cao
authors.forename: Hong-Sheng
authors.initials: HS
authors.lastname: Zhan
mesh_terms: D000818:Animals; D049877:Anodonta / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005936:Glucans / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000378:metabolism; D051381:Rats; D060449:Wnt Signaling Pathway / Q000187:drug effects; D060509:Wnt3A Protein / Q000235:genetics / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism
chemical_list: D005936:Glucans; D053583:Interleukin-1beta; D060509:Wnt3A Protein; D051176:beta Catenin