title: Ultra-high resolution crystal structure of recombinant caprine β-lactoglobulin.
abstract: β-Lactoglobulin (βlg) is the most abundant whey protein in the milks of ruminant animals. While bovine βlg has been subjected to a vast array of studies, little is known about the caprine ortholog. We present an ultra-high resolution crystal structure of caprine βlg complemented by analytical ultracentrifugation and small-angle X-ray scattering data. In both solution and crystalline states caprine βlg is dimeric (K(D)<5 μM); however, our data suggest a flexible quaternary arrangement of subunits within the dimer. These structural findings will provide insight into relationships among structural, processing, nutritional and immunological characteristics that distinguish cow's and goat's milk.
authors.affiliation: Biomolecular Interaction Centre, School of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Crowther
authors.affiliation: Biomolecular Interaction Centre, School of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
authors.forename: Moritz
authors.initials: M
authors.lastname: Lassé
authors.affiliation: Biomolecular Interaction Centre, School of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
authors.forename: Hironori
authors.initials: H
authors.lastname: Suzuki
authors.affiliation: Biomolecular Interaction Centre, School of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Kessans
authors.affiliation: Institute of Fundamental Sciences and The Riddet Institute, Massey University, Palmerston North, New Zealand.
authors.forename: Trevor S
authors.initials: TS
authors.lastname: Loo
authors.affiliation: Institute of Fundamental Sciences and The Riddet Institute, Massey University, Palmerston North, New Zealand.
authors.forename: Gillian E
authors.initials: GE
authors.lastname: Norris
authors.affiliation: Food and Bio-based Products, AgResearch Limited, Ruakura Research Centre, Hamilton, New Zealand.
authors.forename: Alison J
authors.initials: AJ
authors.lastname: Hodgkinson
authors.affiliation: Institute of Fundamental Sciences and The Riddet Institute, Massey University, Palmerston North, New Zealand.
authors.forename: Geoffrey B
authors.initials: GB
authors.lastname: Jameson
authors.affiliation: Biomolecular Interaction Centre, School of Biological Sciences, University of Canterbury, Christchurch, New Zealand; Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, Victoria 3010, Australia. Electronic address: renwick.dobson@canterbury.ac.nz.
authors.forename: Renwick C J
authors.initials: RC
authors.lastname: Dobson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D018360:Crystallography, X-Ray; D006041:Goats; D007782:Lactoglobulins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D008958:Models, Molecular; D008969:Molecular Sequence Data; D020836:Protein Structure, Quaternary; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D007782:Lactoglobulins; D011994:Recombinant Proteins
keywords: Analytical ultracentrifugation; Caprine; Goat; Milk whey; Small-angle X-ray scattering; Ultra-high resolution structure