title: Effects of resistin on ovarian folliculogenesis and steroidogenesis in the vespertilionid bat, Scotophilus heathi.
abstract: The bat Scotophilus heathi exhibit prolonged anovulatory condition known as delayed ovulation coinciding with the period of extensive fat accumulation. The present study was undertaken to find out whether extensive accumulation of fat in S. heathi is responsible for suppression of ovarian activity by increasing production of adipokine resistin in the bat. This was achieved by (a) investigating variation in serum resistin level in relation to the changes in the body fat mass and (b) evaluating the effect of resistin treatment on ovarian activity with reference to steroid synthesis. An attempt was also made to determine whether resistin mediate its effects on ovary through signal transducer and activator of transcription 3 (STAT3) signaling mechanism. The results showed significant seasonal variation in serum resistin level with the peak level coinciding with the period of maximum fat accumulation, high circulating androgen level and period of anovulation. The treatment with resistin to the bat caused increase in androstenedione due to stimulatory effects on 3β-hydroxysteroid dehydrogenase, but decrease in estradiol level due to inhibitory effect on aromatase. Resistin treatment increased androgen receptor protein together with increased insulin receptor but not through conventional luteinizing hormone receptor and steroidogenic acute regulatory protein mediated pathways. This study further showed that resistin treatment increases androstenedione synthesis and up-regulates insulin receptor in the ovary through STAT3 mediated pathways. These findings suggest that obese women through increased resistin synthesis may causes development of non-ovulatory antral follicles through insulin receptor signaling cascade.
authors.affiliation: Reproductive Endocrinology Lab, Department of Zoology, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Ajit
authors.initials: A
authors.lastname: Singh
authors.affiliation: Dr. Reddy's Institute of Life Sciences, Hyderabad University Campus, Gachibowly, Hyderabad 500046, India.
authors.forename: Madhuri
authors.initials: M
authors.lastname: Suragani
authors.affiliation: Reproductive Endocrinology Lab, Department of Zoology, Banaras Hindu University, Varanasi 221005, India. Electronic address: akrishna_ak@yahoo.co.in.
authors.forename: Amitabh
authors.initials: A
authors.lastname: Krishna
mesh_terms: D052242:Adiponectin / Q000097:blood; D050154:Adiposity / Q000187:drug effects; D000735:Androstenedione / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002685:Chiroptera / Q000097:blood / Q000254:growth & development* / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006801:Humans; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D009929:Organ Size / Q000187:drug effects; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D015203:Reproducibility of Results; D052243:Resistin / Q000097:blood / Q000494:pharmacology*; D013256:Steroids / Q000096:biosynthesis / Q000097:blood*
chemical_list: D052242:Adiponectin; D007328:Insulin; D020738:Leptin; D011956:Receptors, Cell Surface; D052243:Resistin; D013256:Steroids; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone
keywords: AR; Insulin; Insulin receptor; Ovary; Resistin; STAT3