title: In situ monitoring of intracellular controlled drug release from mesoporous silica nanoparticles coated with pH-responsive charge-reversal polymer.
abstract: Therapeutic platforms such as chemotherapy that respond to physical and biological stimuli are highly desirable for effective cancer therapy. In this study, pH-responsive charge-reversal, polymer-coated mesoporous silica nanoparticles [PAH-cit/APTES-MSNs; PAH-cit refers to poly(allylamine)-citraconic anhydride; APTES refers to (3-aminopropyl)triethoxysilane] were synthesized for application as drug-delivery systems for the treatment of malignant cells. Confocal laser scanning microscopy (CLSM) revealed that the PAH-cit/APTES-MSNs nanocomposite effectively delivered and released doxorubicin hydrochloride to the nucleus of HeLa (human cervical carcinoma) cells. Additionally, the real-time dynamic drug-release process was monitored by CLSM. The current pH-controlled-smart-release platform holds promise in drug-delivery and cancer therapy-related applications.
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University , Shanghai 200433, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Tong
authors.initials: T
authors.lastname: Wu
authors.forename: Ji-Lie
authors.initials: JL
authors.lastname: Kong
mesh_terms: D016923:Cell Death / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002949:Citraconic Anhydrides / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000494:pharmacology*; D004317:Doxorubicin / Q000494:pharmacology; D065546:Drug Liberation; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D042541:Intracellular Space / Q000737:chemistry*; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011073:Polyamines / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D011437:Propylamines; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002949:Citraconic Anhydrides; D020099:Coated Materials, Biocompatible; D003692:Delayed-Action Preparations; D011073:Polyamines; D011108:Polymers; D011437:Propylamines; D012821:Silanes; C063994:polyallylamine; D012822:Silicon Dioxide; D004317:Doxorubicin; C477625:amino-propyl-triethoxysilane
keywords: doxorubicin hydrochloride; drug delivery; mesoporous silica nanoparticles; pH-controlled release; pH-responsive charge-reversal polymer