title: Evaluation of cancer imaging potential and photodynamic therapy efficacy of copper (II) benzyloxypheophorbide-a.
abstract: The biological potential of a synthetic copper chlorophyll derivative was investigated via in vivo and in vitro experiments. The Cu-chlorophyll derivative photosensitizer (Cu-PH-A) was labeled with (131)I with high efficiency (92.9 ± 4.2%) using the iodogen method. Cell culture studies were performed with the MCF-7 and MDAH-2774 cell lines after radiolabeling. The photosensitizing activity of Cu-PH-A was more effective in MDAH-2774 cells than in MCF-7 cells at a concentration of 50 μM. When the biodistribution in female Albino Wistar rats was examined, uptake of the radiolabeled photosensitizer was maximal in the liver and ovaries after 60 min. It is concluded that radiolabeled Cu-chlorophyll derivative photosensitizer has high uptake in ovaries in normal rats. In addition, the intercellular uptake and PDT efficacy of the Cu-PH-A in MDAH-2774 were good compared with MCF-7 cells. This photosensitizer could be useful for both ovary tumour imaging and PDT.
authors.affiliation: Advanced Technology Research & Application Center, Mersin University, Ciftlikkoy Campus , Mersin , Turkey .
authors.forename: Kasim
authors.initials: K
authors.lastname: Ocakoglu
authors.forename: Özge
authors.initials: Ö
authors.lastname: Er
authors.forename: Fatma
authors.initials: F
authors.lastname: Yurt Lambrecht
authors.forename: Sunde
authors.initials: S
authors.lastname: Yılmaz Süslüer
authors.forename: Cağla
authors.initials: C
authors.lastname: Kayabasi
authors.forename: Cumhur
authors.initials: C
authors.lastname: Gündüz
authors.forename: Osman
authors.initials: O
authors.lastname: Yılmaz
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000493:pharmacokinetics / Q000494:pharmacology; D002735:Chlorophyllides / Q000737:chemistry; D004355:Drug Stability; D005260:Female; D006801:Humans; D057054:Molecular Imaging; D009369:Neoplasms / Q000188:drug therapy*; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000493:pharmacokinetics / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats
chemical_list: D002735:Chlorophyllides; D017319:Photosensitizing Agents; C000602449:copper(II) benzyloxypheophorbide-a; D002734:Chlorophyll; C007020:chlorophyllin
keywords: 131I; chlorophyll-a; metallo-chlorophyll; nuclear imaging; photodynamic therapy