title: Oxidative stability of DHA phenolic ester.
abstract: Docosahexaenoic acid vanillyl ester (DHA-VE) was synthesized from docosahexaenoic acid ethyl ester (DHA-EE) and vanillyl alcohol by a solvent-free alcoholysis process catalysed by Candida antarctica lipase B. Oxidative stability of pure DHA-VE and the crude reaction medium consisting of 45% DHA-VE and 55% DHA-EE were compared with that of DHA-EE under various storage conditions. Oxidation progress was followed by determination of conjugated dienes and FTIR measurements. Analyses showed that DHA-EE was rapidly oxidised under all storage conditions in comparison with DHA-VE and crude reaction medium, whatever the temperature and the storage time. The grafting of vanillyl alcohol appeared as a powerful way to stabilize DHA against oxidation. Thanks to their stability, both DHA-VE and the crude reaction medium, allowing the production of the ester, offer huge potential as functional ingredients.
authors.affiliation: Université de Lorraine, Laboratoire Ingénierie des Biomolécules (LIBio), 2 av. de la Forêt d'Haye, TSA 40602, 54518 Vandoeuvre Cedex, France; Fayoum University, Food Science and Technology Dept., Faculty of Agriculture, 63514 Fayoum, Egypt. Electronic address: mhr00@fayoum.edu.eg.
authors.forename: Mohamed H H
authors.initials: MH
authors.lastname: Roby
authors.affiliation: Federal University of Viçosa, Department of Food Technology, Av Peter Henry Rolfs s/n 36570-900, Viçosa, Minas Gerais, Brazil.
authors.forename: Vanessa C
authors.initials: VC
authors.lastname: De Castro
authors.affiliation: Federal University of Viçosa, Department of Food Technology, Av Peter Henry Rolfs s/n 36570-900, Viçosa, Minas Gerais, Brazil.
authors.forename: Brenda N
authors.initials: BN
authors.lastname: Targino
authors.affiliation: Federal University of Viçosa, Department of Food Technology, Av Peter Henry Rolfs s/n 36570-900, Viçosa, Minas Gerais, Brazil.
authors.forename: Paulo H
authors.initials: PH
authors.lastname: Alves Da Silva
authors.affiliation: Université de Lorraine, Laboratoire Ingénierie des Biomolécules (LIBio), 2 av. de la Forêt d'Haye, TSA 40602, 54518 Vandoeuvre Cedex, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Mangavel
authors.affiliation: Université de Lorraine, UMR 7565 SRSMC, F-54606 Nancy, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Chretien
authors.affiliation: Université de Lorraine, Laboratoire Ingénierie des Biomolécules (LIBio), 2 av. de la Forêt d'Haye, TSA 40602, 54518 Vandoeuvre Cedex, France. Electronic address: catherine.humeau@univ-lorraine.fr.
authors.forename: Catherine
authors.initials: C
authors.lastname: Humeau
authors.affiliation: Université de Lorraine, Laboratoire Ingénierie des Biomolécules (LIBio), 2 av. de la Forêt d'Haye, TSA 40602, 54518 Vandoeuvre Cedex, France. Electronic address: stephane.desobry@univ-lorraine.fr.
authors.forename: Stephane
authors.initials: S
authors.lastname: Desobry
mesh_terms: D001592:Benzyl Alcohols / Q000737:chemistry; D004281:Docosahexaenoic Acids / Q000737:chemistry*; D004952:Esters / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: D001592:Benzyl Alcohols; D004952:Esters; D005656:Fungal Proteins; D004281:Docosahexaenoic Acids; C067518:4,7,10,13,16,19-docosahexaenoic acid ethyl ester; D008049:Lipase; C431349:lipase B, Candida antarctica; C024078:vanillyl alcohol
keywords: DHA and vanillyl alcohol; Enzymatic esterification; FTIR spectroscopy omega-3; Lipase; Oxidation; Oxidative stability; PUFA