title: Comparison of the effect of post-heparin and pre-heparin lipoprotein lipase and hepatic triglyceride lipase on remnant lipoprotein metabolism.
abstract: BACKGROUND
  A comparison of post-heparin and pre-heparin plasma lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) on the metabolism of remnant lipoproteins (RLPs) has not been reported yet.
  
  
  METHODS
  Healthy volunteers were injected with heparin for LPL and HTGL determination in the fasting (8:00) and postprandial (20:00) plasma on the same day. Plasma total cholesterol (TC), triglycerides (TG), LDL-C, HDL-C, small dense LDL (sdLDL)-C, remnant lipoprotein (RLP)-C, RLP-TG, the RLP-TG/RLP-C ratio, adiponectin and apoCIII were measured.
  
  
  RESULTS
  LPL activity and concentration in the post-heparin plasma exhibited a significant inverse correlation with TG, RLP-C, RLP-TG, and RLP particle size estimated as RLP-TG/RLP-C ratio and sdLDL-C, and positively correlated with HDL-C. HTGL was only inversely correlated with HDL-C. LPL concentration in the pre-heparin plasma was also inversely correlated with the RLP-TG/RLP-C ratio and other lipoprotein parameters. Adiponectin was inversely correlated with RLP-TG/RLP-C ratio and apoC III was positively correlated with RLP-TG/RLP-C ratio, but not correlated with LPL activity.
  
  
  CONCLUSIONS
  LPL activity and concentration were inversely and significantly correlated with the particle size of RLP in both the post-heparin and pre-heparin plasma. Those results suggest that LPL concentration in pre-heparin plasma can take the place of LPL activity in the post-heparin plasma.
authors.affiliation: Diabetes and Metabolic Disease Research Center, Hidaka Hospital, Takasaki, Gunma, Japan; Department of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan. Electronic address: takahsi830530@yahoo.co.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Shirakawa
authors.affiliation: Diabetes and Metabolic Disease Research Center, Hidaka Hospital, Takasaki, Gunma, Japan; Department of General Medicine, Kanazawa Medical University, Kanazawa, Ishikawa, Japan; Department of Molecular Biosciences, School of Veterinary Medicine and Department of Nutrition, University of California, Davis, CA, USA.
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Nakajima
authors.affiliation: Diabetes and Metabolic Disease Research Center, Hidaka Hospital, Takasaki, Gunma, Japan.
authors.forename: Younosuke
authors.initials: Y
authors.lastname: Shimomura
authors.affiliation: Department of General Medicine, Kanazawa Medical University, Kanazawa, Ishikawa, Japan.
authors.forename: Junji
authors.initials: J
authors.lastname: Kobayashi
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine and Department of Nutrition, University of California, Davis, CA, USA.
authors.forename: Kimber
authors.initials: K
authors.lastname: Stanhope
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine and Department of Nutrition, University of California, Davis, CA, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Havel
authors.affiliation: Department of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Machida
authors.affiliation: Department of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sumino
authors.affiliation: Department of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Murakami
mesh_terms: D000328:Adult; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D005215:Fasting; D005260:Female; D006493:Heparin / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D016014:Linear Models; D008049:Lipase / Q000097:blood* / Q000378:metabolism; D008071:Lipoprotein Lipase / Q000097:blood* / Q000378:metabolism; D008074:Lipoproteins / Q000097:blood / Q000378:metabolism*; D008297:Male; D010316:Particle Size; D019518:Postprandial Period
chemical_list: D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D008074:Lipoproteins; D006493:Heparin; D008049:Lipase; D008071:Lipoprotein Lipase
keywords: Hepatic triglyceride lipase (HTGL); Lipoprotein lipase (LPL); Post-heparin plasma; Pre-heparin plasma; RLP-TG/RLP-C ratio; Remnant lipoproteins (RLP)