title: Monitoring of the environment at the transplant unit-hemato-oncology clinic.
abstract: OBJECTIVE
  Aim of this study was to monitor the environment at the Transplant Unit-Hemato-Oncology Clinic, University Hospital Olomouc (Olomouc, Czech Republic) and identify risks for the patients.
  
  
  RESULTS
  Microorganisms were cultivated under standard aerobic conditions. Strains were biochemically identified using the BD Phoenix™ PID Panel (USA). Legionella pneumophila was identified by DNA sequencing. From the air, the most frequently isolated strains were coagulase-negative staphylococci (94.3%), Micrococcus spp. and Bacillus spp. No Gram-negative strains were isolated from the air. From the surfaces, the most frequently isolated Gram-positive strains were coagulase-negative staphylococci (67.4%), Bacillus spp., enterococci (5.5%), Staphylococcus aureus (2.3%) and Micrococcus spp. (1.7%). From the surfaces, the most frequently isolated Gram-negative strains were from genera Pseudomonas (28%), Enterobacter (28%), E. coli (6%), and Klebsiella spp. (5%). From the personnel, the most frequently isolated Gram-positive strains were coagulase-negative staphylococci (59.6%), Bacillus spp. (24.1%) and Staphylococcus aureus (9.8%). From the personnel, the most frequently isolated Gram-negative strains were Enterobacter spp. (61%), Klebsiella oxytoca (18%), and E. coli (11%). Microscopic filamentous fungi were isolated in 13 cases (2.71%). Isolated strains were Aspergillus spp. (4), Trichoderma spp. (2), Penicillium spp. (2), one case of the strains Paecilomyces spp., Eurotium spp., Monilia spp.
  
  
  CONCLUSIONS
  The study found no significant deviations in the microbial contamination of the cleanroom air. The personnel entrance of the Transplant Unit represent a high risk area, an extreme value (7270 CFU/m3) was recorded. Regime measures are fully effective, no other deficiencies were found.
  
  
  CONCLUSIONS
  This epidemiological study, which was held for the duration of one year at the Transplant Unit-Hemato-Oncology Clinic, University Hospital Olomouc. The study monitored microbial contamination of the cleanroom air, surfaces, water, colonization of the personnel by bacterial strains of epidemiological consequence.
authors.affiliation: Department of Preventive Medicine, Faculty of Medicine and Dentistry, Palacký University Olomouc, Olomouc 775 15, Czech Republic. ivanka.matouskova@upol.cz.
authors.forename: Ivanka
authors.initials: I
authors.lastname: Matoušková
authors.affiliation: Department of Preventive Medicine, Faculty of Medicine and Dentistry, Palacký University Olomouc, Olomouc 775 15, Czech Republic. holy.ondrej@seznam.cz.
authors.forename: Ondřej
authors.initials: O
authors.lastname: Holy
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D018153:Czech Republic; D004783:Environmental Microbiology; D006757:Hospital Units; D006785:Hospitals, University; D006801:Humans; D018570:Risk Assessment; D033581:Stem Cell Transplantation