title: A model of spontaneous mouse mammary tumor for human estrogen receptor- and progesterone receptor-negative breast cancer.
abstract: Breast cancer (BC) is the most frequently malignancy in women. Therefore, establishment of an animal model for the development of preventative measures and effective treatment for tumors is required. A novel heterogeneous spontaneous mammary tumor animal model of Kunming mice was generated. The purpose of this study was to characterize the spontaneous mammary tumor model. Histopathologically, invasive nodular masses of pleomorphic tubular neoplastic epithelial cells invaded fibro-vascular stroma, adjacent dermis and muscle tissue. Metastatic spread through blood vessel into liver and lungs was observed by hematoxylin eosin staining. No estrogen receptor (ER) or progesterone receptor (PR) immunoreactivity was detected in their associated malignant tumors, human epidermal growth factor receptor-2 (HER-2) protein weak expression was found by immunohistochemistry. High expression of vascular endothelial growth factor (VEGF), moderate or high expression of c-Myc and cyclin D1 were observed in tumor sections at different stages (2, 4, 6 and 8 weeks after cancer being found) when compared with that of the normal mammary glands. The result showed that the model is of an invasive ductal carcinoma. Remarkably in the mouse model, ER and PR-negative and HER2 weak positivity are observed. The high or moderate expressions of breast cancer markers (VEGF, c-Myc and cyclin D1) in mammary cancer tissue change at different stages. To our knowledge, this is the first report of a spontaneous mammary model displaying colony-strain, outbred mice. This model will be an attractive tool to understand the biology of anti-hormonal breast cancer in women.
authors.affiliation: School of Basic Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, P.R. China.
authors.forename: Lixiang
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Jiangxi University of TCM Science and Technology, Nanchang, Jiangxi 330004, P.R. China.
authors.forename: Bugao
authors.initials: B
authors.lastname: Zhou
authors.affiliation: School of Basic Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, P.R. China.
authors.forename: Xianming
authors.initials: X
authors.lastname: Meng
authors.affiliation: Key Laboratory of Modern Preparation of TCM Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, P.R. China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Zhu
authors.affiliation: School of Basic Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, P.R. China.
authors.forename: Airen
authors.initials: A
authors.lastname: Zuo
authors.affiliation: School of Basic Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, P.R. China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Basic Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, P.R. China.
authors.forename: Runde
authors.initials: R
authors.lastname: Jiang
authors.affiliation: Department of Scientific Research, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, P.R. China.
authors.forename: Shiping
authors.initials: S
authors.lastname: Yu
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000096:biosynthesis; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D019938:Cyclin D1 / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D015674:Mammary Neoplasms, Animal / Q000235:genetics* / Q000473:pathology; D051379:Mice; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics; D011960:Receptors, Estrogen / Q000235:genetics; D011980:Receptors, Progesterone / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
chemical_list: D014408:Biomarkers, Tumor; D019938:Cyclin D1; D016271:Proto-Oncogene Proteins c-myc; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; C508053:ERBB2 protein, human