title: In vitro digestion of starches in a dynamic gastrointestinal model: an innovative study to optimize dietary management of patients with hepatic glycogen storage diseases.
abstract: Uncooked cornstarch (UCCS) is a widely used treatment strategy for patients with hepatic glycogen storage disease (GSD). It has been observed that GSD-patients display different metabolic responses to different cornstarches. The objective was to characterize starch fractions and analyze the digestion of different starches in a dynamic gastrointestinal in vitro model. The following brands of UCCS were studied: Argo and Great Value from the United States of America; Brazilian Maizena Duryea and Yoki from Brazil; Dutch Maizena Duryea from the Netherlands. Glycosade, a modified starch, and sweet polvilho, a Brazilian starch extracted from cassava, were also studied. The starch fractions were analyzed by glycemic TNO index method and digestion analyses were determined by the TIM-1 system, a dynamic, computer-controlled, in vitro gastrointestinal model, which simulates the stomach and small intestine. The final digested amounts were between 84 and 86% for the UCCS and Glycosade, but was 75.5% for sweet povilho. At 180 min of the experiment, an important time-point for GSD patients, the digested amount of the starches corresponded to 67.9-71.5 for the UCCS and Glycosade, while it was 55.5% for sweet povilho. In an experiment with a mixture of sweet polvilho and Brazilian Maizena Duryea, a final digested amount of 78.4% was found, while the value at 180 min was 61.7%. Sweet polvilho seems to have a slower and extended release of glucose and looks like an interesting product to be further studied as it might lead to extended normoglycemia in GSD-patients.
authors.affiliation: Post-Graduation Program in Genetics and Molecular Biology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Tatiéle
authors.initials: T
authors.lastname: Nalin
authors.forename: Koen
authors.initials: K
authors.lastname: Venema
authors.forename: David A
authors.initials: DA
authors.lastname: Weinstein
authors.forename: Carolina F M
authors.initials: CF
authors.lastname: de Souza
authors.forename: Ingrid D S
authors.initials: ID
authors.lastname: Perry
authors.forename: Mario T R
authors.initials: MT
authors.lastname: van Wandelen
authors.forename: Margreet
authors.initials: M
authors.lastname: van Rijn
authors.forename: G Peter A
authors.initials: GP
authors.lastname: Smit
authors.forename: Ida V D
authors.initials: IV
authors.lastname: Schwartz
authors.forename: Terry G J
authors.initials: TG
authors.lastname: Derks
mesh_terms: D004063:Digestion / Q000502:physiology*; D005947:Glucose / Q000378:metabolism*; D005953:Glycogen Storage Disease Type I / Q000178:diet therapy*; D006801:Humans; D008954:Models, Biological; D013213:Starch / Q000032:analysis* / Q000145:classification* / Q000627:therapeutic use
chemical_list: D013213:Starch; D005947:Glucose