title: Frequency of EGFR mutations in lung adenocarcinoma with malignant pleural effusion: Implication of cancer biological behaviour regulated by EGFR mutation.
abstract: OBJECTIVE
  A retrospective single-centre study to compare the clinical features of patients with lung adenocarcinoma with and without epidermal growth factor receptor (EGFR) mutations.
  
  
  METHODS
  Pretreatment medical records of patients with lung adenocarcinoma were reviewed. DNA was extracted from paraffin wax-embedded tumour tissue for analysis of EGFR mutations. Malignant pleural effusion (MPE) was diagnosed by cytopathological testing of pleural fluid.
  
  
  RESULTS
  EGFR mutations (19-Del and L858R) were recorded in 81/283 patients (28.6%). MPE was found in 42/283 patients (14.8%). In patients with stage IV disease, the frequency of EGFR mutations was higher in those with MPE than in those without MPE. EGFR mutations were independently associated with female sex, no history of smoking and presence of MPE.
  
  
  CONCLUSIONS
  There was a positive association between EGFR mutation and the presence of MPE. EGFR mutations may play an important role in the formation of MPE.
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
authors.forename: JianYong
authors.initials: J
authors.lastname: Zou
authors.affiliation: Department of Thoracic Surgery, Cancer Centre of Sun Yat-Sen University, Guangzhou, China.
authors.forename: Amos Ela
authors.initials: AE
authors.lastname: Bella
authors.affiliation: Department of Cardiothoracic Surgery, Huangpu Branch of the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
authors.forename: ZhenGuang
authors.initials: Z
authors.lastname: Chen
authors.affiliation: The Department of Thoracic Surgery, The First Affiliated Hospital of NanYang Medical College, HeNan, China.
authors.forename: XiangQian
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
authors.forename: ChunHua
authors.initials: C
authors.lastname: Su
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
authors.forename: YiYan
authors.initials: Y
authors.lastname: Lei
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China 179235907@qq.com.
authors.forename: HongHe
authors.initials: H
authors.lastname: Luo
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D004252:DNA Mutational Analysis; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D009367:Neoplasm Staging; D016066:Pleural Effusion, Malignant / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D011379:Prognosis; D012189:Retrospective Studies
chemical_list: C512478:EGFR protein, human; D066246:ErbB Receptors
keywords: EGFR mutation; Lung adenocarcinoma; malignant pleural effusion