title: Developing conversed microsatellite markers and their implications in evolutionary analysis of the Bemisia tabaci complex.
abstract: The study of population genetics among the Bemisia tabaci complex is limited due to the lack of conserved molecular markers. In this study, 358, 433 and 322 new polynucleotide microsatellites are separately identified from the transcriptome sequences of three cryptic species of the B. tabaci complex. The cross species transferability of 57 microsatellites was then experimentally validated. The results indicate that these markers are conserved and have high inter-taxon transferability. Thirteen markers were employed to assess the genetic relationships among six cryptic species of the B. tabaci complex. To our surprise, the inferred phylogeny was consistent with that of mitochondrial COI sequences, indicating that microsatellites have the potential to distinguish species of the B. tabaci complex. Our results demonstrate that development of microsatellites from transcriptome data is a fast and cost-effective approach. These markers can be used to analyze the population genetics and evolutionary patterns of the B. tabaci complex.
authors.affiliation: Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Hua-Ling
authors.initials: HL
authors.lastname: Wang
authors.affiliation: Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Yang
authors.affiliation: ARC Centre of Excellence, Plant Energy Biology, The University of Western Australia, 35 Stirling Highway, Crawley 6009 Western Australia.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Boykin
authors.affiliation: Queensland Brain Institute, University of Queensland, Brisbane, Qld 4072, Australia.
authors.forename: Qiong-Yi
authors.initials: QY
authors.lastname: Zhao
authors.affiliation: Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yu-Jun
authors.initials: YJ
authors.lastname: Wang
authors.affiliation: Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Shu-Sheng
authors.initials: SS
authors.lastname: Liu
authors.affiliation: Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D005828:Genetics, Population; D006430:Hemiptera / Q000235:genetics* / Q000254:growth & development; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D012333:RNA, Messenger