title: Adsorption induced denaturation: application to denaturation of soybean trypsin inhibitor (SBTI) and lipoxygenase (LOX) in soymilk.
abstract: Allyl glycidyl ether-ethylene glycol dimethacrylate copolymer with 25 % crosslink density has been functionalized with various amines. The polymer bearing a dibenzyl amino function efficiently removed the soybean trypsin inhibitor (SBTI) and lipoxygenase (LOX) from soymilk. The polymer binds SBTI and LOX efficiently (25-30 mg/g) through a combination of hydrophobic and ionic interactions and denatures them at room temperature by simple contact for 1 h. The bound proteins can be easily recovered almost quantitatively by elution with 1 % (v/v) acetic acid, and the polymer can be recycled at least 10 times without significant changes in binding capacity.
authors.affiliation: Natural Products Chemistry Division, Indian Institute of Chemical Technology, Uppal Road, Habsiguda, Hyderabad, 500007, India.
authors.forename: Lakshmi Swarnalatha
authors.initials: LS
authors.lastname: Jasti
authors.forename: Kuna
authors.initials: K
authors.lastname: Lavanya
authors.forename: Nitin W
authors.initials: NW
authors.lastname: Fadnavis
mesh_terms: D000327:Adsorption; D004852:Epoxy Compounds / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008084:Lipoxygenase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D008689:Methacrylates / Q000737:chemistry; D011489:Protein Denaturation; D041942:Soy Milk / Q000737:chemistry*; D030262:Soybean Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D004852:Epoxy Compounds; D008689:Methacrylates; D030262:Soybean Proteins; D014361:Trypsin Inhibitors; C004919:ethylene dimethacrylate; D008084:Lipoxygenase; C054361:allyl glycidyl ether