title: Characterization of mustard seeds and paste by DART ionization with time-of-flight mass spectrometry.
abstract: Direct analysis in real time (DART) is a novel technique with great potential for rapid screening analysis. The DART ionization method coupled with high-resolution time-of-flight mass spectrometry (TOF-MS) has been used for characterization of mustard seeds and table mustard. The possibility to use DART to analyse glucosinolates was confirmed on determination of sinalbin (4-hydroxybenzyl glucosinolate). The DART-TOF-MS method was optimized and validated. A set of samples of mustard seeds and mustard products was analyzed. High-performance liquid chromatography and DART-TOF-MS were used to determine glucosinolates in mustard seeds and compared. The correlation equation between these methods was DART = 0.797*HPLC + 6.987, R(2)  = 0.972. The DART technique seems to be a suitable method for evaluation of the quality of mustard seeds and mustard products.
authors.affiliation: Institute of Chemical Technology Prague, Department of Food Preservation, Prague.
authors.forename: Jana
authors.initials: J
authors.lastname: Prchalová
authors.forename: František
authors.initials: F
authors.lastname: Kovařík
authors.forename: Rudolf
authors.initials: R
authors.lastname: Ševčík
authors.forename: Helena
authors.initials: H
authors.lastname: Čížková
authors.forename: Aleš
authors.initials: A
authors.lastname: Rajchl
keywords: DART; HPLC; fingerprints; mass spectrometry; mustard; mustard seed; sinalbin