title: IL-17-expressing CD4⁺ and CD8⁺ T lymphocytes in human toxoplasmosis.
abstract: This study aimed to measure the synthesis of Th1 and Th2 cytokines by mononuclear cells after culture with live T. gondii and identified Th17 (CD4(+)) and Tc17 (CD8(+)) cells in toxoplasma-seronegative and toxoplasma-seropositive parturient and nonpregnant women. Cytometric bead arrays were used to measure cytokine levels (IL-2, TNF-α, IFN-γ, IL-4, IL-5, and IL-10); immunophenotyping was used to characterize Th17 and Tc17 cells, and the cells were stained with antibodies against CD4(+) and CD8(+) T cells expressing IL-17. The addition of tachyzoites to cell cultures induced the synthesis of IL-5, IL-10, and TNF-α by cells from seronegative parturient women and of IL-5 and IL-10 by cells from seropositive, nonpregnant women. We observed a lower level of IL-17-expressing CD4(+) and CD8(+) T lymphocytes in cultures of cells from seronegative and seropositive parturient and nonpregnant women that were stimulated with tachyzoites, whereas analysis of the CD4(+) and CD8(+) T cell populations showed a higher level of CD4(+) T cells compared with CD8(+) T cells. These results suggest that the cytokine pattern and IL-17-expressing CD4(+) and CD8(+) T lymphocytes may have important roles in the inflammatory response to T. gondii, thus contributing to the maintenance of pregnancy and control of parasite invasion and replication.
authors.affiliation: Course of Tropical Medicine and Infectology, Laboratory of Immunology, Federal University of Triângulo Mineiro, Avenida Frei Paulino, No. 30, 38025-180 Uberaba, MG, Brazil.
authors.forename: Jéssica Líver Alves
authors.initials: JL
authors.lastname: Silva
authors.affiliation: Laboratory of Biomedical Sciences, Federal University of Uberlândia, Rua 20, No. 1600, 38304-402 Ituiutaba, MG, Brazil.
authors.forename: Karine
authors.identifier: 0000-0003-0005-209X
authors.initials: K
authors.lastname: Rezende-Oliveira
authors.affiliation: Course of Tropical Medicine and Infectology, Laboratory of Immunology, Federal University of Triângulo Mineiro, Avenida Frei Paulino, No. 30, 38025-180 Uberaba, MG, Brazil.
authors.forename: Marcos Vinicius
authors.initials: MV
authors.lastname: da Silva
authors.affiliation: Course of Tropical Medicine and Infectology, Laboratory of Parasitology, Federal University of Triângulo Mineiro, Avenida Frei Paulino, No. 30, 38025-180 Uberaba, MG, Brazil.
authors.forename: César
authors.initials: C
authors.lastname: Gómez-Hernández
authors.affiliation: Course of Tropical Medicine and Infectology, Laboratory of Immunology, Federal University of Triângulo Mineiro, Avenida Frei Paulino, No. 30, 38025-180 Uberaba, MG, Brazil.
authors.forename: Bethânea Crema
authors.initials: BC
authors.lastname: Peghini
authors.affiliation: Laboratory of Histology and Embryology, Federal University of Uberlândia, Avenida Pará, No. 1720, 38400-902 Uberlândia, MG, Brazil.
authors.forename: Neide Maria
authors.initials: NM
authors.lastname: Silva
authors.affiliation: Laboratory of Immunology, Federal University of Uberlândia, Avenida Pará, No. 1720, 38400-902 Uberlândia, MG, Brazil.
authors.forename: José Roberto
authors.initials: JR
authors.lastname: Mineo
authors.affiliation: Course of Tropical Medicine and Infectology, Laboratory of Immunology, Federal University of Triângulo Mineiro, Avenida Frei Paulino, No. 30, 38025-180 Uberaba, MG, Brazil.
authors.forename: Virmondes
authors.initials: V
authors.lastname: Rodrigues Júnior
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D020381:Interleukin-17 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear; D008875:Middle Aged; D014123:Toxoplasmosis / Q000276:immunology* / Q000378:metabolism*
chemical_list: D020381:Interleukin-17