title: CD44 is required for spatial memory retention and sensorimotor functions.
abstract: CD44 is a transmembrane receptor for the glycosaminoglycan hyaluronan, a component of the extracellular matrix. CD44 is expressed by neural stem/progenitor cells, astrocytes, and some neurons but its function in the central nervous system is unknown. To determine the role of CD44 in brain function, we behaviorally analyzed CD44-null (KO) and wild-type (WT) mice. KO mice showed increased activity levels in the light-dark test and a trend toward increased activity in the open field. In addition, KO mice showed impaired hippocampus-dependent spatial memory retention in the probe trial following the first hidden-platform training day in the Morris water maze: WT mice showed spatial memory retention and spent more time in the target quadrant than any other quadrant, while KO mice did not. Although there were no genotype differences in swim speeds during the water maze training sessions with the visible or hidden platform, sensorimotor impairments were seen in other behavioral tests. In the inclined screen and balance beam tests, KO mice moved less than WT mice. In the wire hang test, KO mice also fell off of the wire faster than WT mice. In contrast, there was no genotype difference when emotional learning and memory were assessed in the passive avoidance test. These data support an important role for CD44 in locomotor and sensorimotor functions, and in spatial memory retention.
authors.affiliation: Department of Behavioral Neuroscience, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA; Departments of Neurology and Radiation Medicine, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA; Division of Neuroscience, Oregon National Primate Research Center, 505 NW 185th Avenue, Beaverton, OR 97006, USA.
authors.forename: Jacob
authors.initials: J
authors.lastname: Raber
authors.affiliation: Department of Behavioral Neuroscience, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA.
authors.forename: Reid H J
authors.initials: RH
authors.lastname: Olsen
authors.affiliation: Division of Neuroscience, Oregon National Primate Research Center, 505 NW 185th Avenue, Beaverton, OR 97006, USA.
authors.forename: Weiping
authors.initials: W
authors.lastname: Su
authors.affiliation: Division of Neuroscience, Oregon National Primate Research Center, 505 NW 185th Avenue, Beaverton, OR 97006, USA.
authors.forename: Scott
authors.initials: S
authors.lastname: Foster
authors.affiliation: Division of Neuroscience, Oregon National Primate Research Center, 505 NW 185th Avenue, Beaverton, OR 97006, USA.
authors.forename: Rubing
authors.initials: R
authors.lastname: Xing
authors.affiliation: Department of Behavioral Neuroscience, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA.
authors.forename: Summer F
authors.initials: SF
authors.lastname: Acevedo
authors.affiliation: Division of Neuroscience, Oregon National Primate Research Center, 505 NW 185th Avenue, Beaverton, OR 97006, USA; Department of Cell and Developmental Biology, School of Medicine, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA. Electronic address: shermanl@ohsu.edu.
authors.forename: Larry S
authors.initials: LS
authors.lastname: Sherman
mesh_terms: D000818:Animals; D001362:Avoidance Learning / Q000502:physiology; D005106:Exploratory Behavior / Q000502:physiology; D005260:Female; D018960:Hyaluronan Receptors / Q000235:genetics / Q000378:metabolism*; D008297:Male; D018782:Maze Learning / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D065852:Spatial Memory / Q000502:physiology*; D018709:Statistics, Nonparametric
chemical_list: C476399:Cd44 protein, mouse; D018960:Hyaluronan Receptors
keywords: CD44; Locomotor; Memory; Mouse; Sensorimotor