title: Detection of PEr 1 extended-spectrum β-lactamase among nosocomial providencia stuartii isolates in Tunisia.
abstract: BACKGROUND
  The Emergence of extended-spectrum beta-lactamases (ESBLs) in Enterobacteriace species imparting resistance to thirdgeneration cephalosporins is a growing concern worldwide. aims: This study was undertaken to characterize ESBL producers from clinical isolates of Providencia stuartii in Tunisia. methods: We analysed ten non-duplicate ESBL positive, P. stuartii isolates collected in intensive care units of military Hospital in Tunisia from blood specimens and presented an extended-spectrum resistance phenotype . the presence of the blaPER-1 encoding genes was confirmed by polymerase chain reaction. results:We detected PER-1 in three of ten isolates of P. stuartii. The gene coding for this enzyme was located on a 100 kb conjugative plasmid that encoded a β-lactamase with a pI of 5.3.
  
  
  CONCLUSIONS
  This study suggests the spreading of this gene among P. stuartii. clinical strains in the next year in Tunisia.
authors.forename: Hella
authors.initials: H
authors.lastname: Lahlaoui
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ben Moussa
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D002511:Cephalosporins / Q000627:therapeutic use; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology*; D006772:Hospitals, Military; D006801:Humans; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D011532:Providencia / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014416:Tunisia / Q000453:epidemiology; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D001618:beta-Lactamases