title: The RANK/ RANKL/ OPG interaction in the repair of autogenous bone grafts in female rats with estrogen deficiency.
abstract: The aim of this study was to evaluate the resorption process during the repair of autogenous bone grafts with or without coverage by an expanded polytetrafluoroethylene (e-PTFE) membrane in female rats with estrogen deficiency using the immunohistochemical technique. Eighty female rats were randomly divided into two groups (OVX and SHAM). The 40 female rats in the OVX group were subjected to ovariectomy, and the 40 female rats in the SHAM group were subjected to simulated ovariectomy. The two groups were further divided in subgroup E, which was subjected to surgery for placement of autogenous bone graft (ABG), and subgroup ME, in which the ABG was covered with an e-PTFE membrane. The animals were killed at 0, 7, 21, 45 and 60 days. The specimens were analyzed using immunohistochemistry for the bone resorption markers RANK, RANK-L and Osteoprotegerin (OPG). A higher remodeling rate was observed at 7 and 21 days after the autogenous bone grafts, when the markers were more intensely expressed. At the final time point, the specimens presented similar characteristics to those observed at the initial time point. The expression of immunohistochemical markers was not altered by the estrogen deficiency. The presence of the e-PTFE membrane delayed the bone resorption process, influencing the immunohistochemical expression of markers.
authors.affiliation: Department of Bioscience and Oral Diagnosis, Instituto de Ciência e Tecnologia ? ICT, Univ Estadual Paulista ? UNESP, São José dos Campos, SP, Brazil.
authors.forename: Tábata de Mello
authors.initials: Tde M
authors.lastname: Tera
authors.affiliation: Department of Bioscience and Oral Diagnosis, Instituto de Ciência e Tecnologia ? ICT, Univ Estadual Paulista ? UNESP, São José dos Campos, SP, Brazil.
authors.forename: Renata Falchete do
authors.initials: RF
authors.lastname: Prado
authors.affiliation: Department of Diagnosis and Surgery, Instituto de Ciência e Tecnologia ? ICT, Univ Estadual Paulista ? UNESP, São José dos Campos, SP, Brazil.
authors.forename: Andrea Carvalho
authors.initials: AC
authors.lastname: De Marco
authors.affiliation: Department of Diagnosis and Surgery, Instituto de Ciência e Tecnologia ? ICT, Univ Estadual Paulista ? UNESP, São José dos Campos, SP, Brazil.
authors.forename: Mauro Pedrine
authors.initials: MP
authors.lastname: Santamaria
authors.affiliation: Department of Diagnosis and Surgery, Instituto de Ciência e Tecnologia ? ICT, Univ Estadual Paulista ? UNESP, São José dos Campos, SP, Brazil.
authors.forename: Maria Aparecida Neves
authors.initials: MA
authors.lastname: Jardini
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001861:Bone Regeneration / Q000502:physiology*; D001862:Bone Resorption / Q000503:physiopathology; D016025:Bone Transplantation; D004967:Estrogens / Q000172:deficiency*; D005260:Female; D007150:Immunohistochemistry; D010010:Osteoclasts / Q000502:physiology; D053244:Osteoprotegerin / Q000502:physiology*; D010052:Ovariectomy; D053245:RANK Ligand / Q000502:physiology*; D011897:Random Allocation; D017208:Rats, Wistar; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000502:physiology*; D013997:Time Factors; D014182:Transplantation, Autologous
chemical_list: D015415:Biomarkers; D004967:Estrogens; D053244:Osteoprotegerin; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B