title: Cell membrane-mimicking coating for blood-contacting polyurethanes.
abstract: The aim of the present work was to develop simple modification technique for polyurethanes (PUs) intended for use in blood-contacting implants (vascular grafts, heart prosthesis, ventricular assist devices). PU surface was modified with soybean-derived phosphatidylcholine (PC) via one-step dip coating technique. In order to evaluate blood compatibility of the obtained materials, samples were contacted with human blood under static and arterial flow-simulated conditions. The PC-modified surfaces were thoroughly characterized and tested for fibrinogen resistance, the ability to resist platelet adhesion and activation, hemolysis percentage and plasma recalcification time. Results demonstrated significant, more than three-fold reduction in the amount of fibrinogen adsorbed to PC-modified materials as compared to non-modified PU. Analysis of the samples' surface after incubation with blood showed high reduction in platelet adhesion. The results were confirmed by analysis of blood samples collected after shear-stress tests--the percentage of free (non-aggregated) platelets remaining in blood samples contacted with PC-coated materials exceeded 70%. The same parameter measured for non-modified PU was significantly lower and equaled 28%.
authors.affiliation: Laboratory of Biomedical Engineering, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warsaw, Poland b.butruk@ichip.pw.edu.pl.
authors.forename: Beata
authors.initials: B
authors.lastname: Butruk-Raszeja
authors.affiliation: Laboratory of Biomedical Engineering, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warsaw, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Trzaskowski
authors.affiliation: Laboratory of Biomedical Engineering, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warsaw, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Ciach
mesh_terms: D000327:Adsorption; D001790:Blood Physiological Phenomena / Q000187:drug effects*; D002462:Cell Membrane / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008422:Materials Testing; D015539:Platelet Activation / Q000187:drug effects / Q000502:physiology; D011140:Polyurethanes / Q000737:chemistry* / Q000494:pharmacology; D013499:Surface Properties / Q000187:drug effects; D017155:Wettability / Q000187:drug effects
chemical_list: D020099:Coated Materials, Biocompatible; D011140:Polyurethanes
keywords: Polyurethane; biomimicry; blood compatibility; fibrinogen; phosphatidylcholine; platelet; surface modification