title: Peroxidase-mimicking DNAzyme modulated growth of CdS nanocrystalline structures in situ through redox reaction: application to development of genosensors and aptasensors.
abstract: This work demonstrates the use of the peroxidase-mimicking DNAzyme (peroxidase-DNAzyme) as general and inexpensive platform for development of fluorogenic assays that do not require organic fluorophores. The system is based on the affinity interaction between the peroxidase-DNAzyme bearing hairpin sequence and the analyte (DNA or low molecular weight molecule), which changes the folding of the hairpin structure and consequently the activity of peroxidase-DNAzyme. Hence, in the presence of the analyte the peroxidase-DNAzyme structure is disrupted and does not catalyze the aerobic oxidation of l-cysteine to cystine. Thus, l-cysteine is not removed from the system and the fluorescence of the assay increases due to the in situ formation of fluorescent CdS nanocrystals. The capability of the system as a platform for fluorogenic assays was demonstrated through designing model geno- and aptasensor for the detection of a tumor marker DNA and a low molecular weight analyte, adenosine 5'triphosphate (ATP), respectively.
authors.affiliation: Biofunctional Nanomaterials Unit, CIC BiomaGUNE , Parque Tecnológico de San Sebastian, Paseo Miramón 182, Donostia-San Sebastián, 20009, Spain.
authors.forename: Gaizka
authors.initials: G
authors.lastname: Garai-Ibabe
authors.forename: Marco
authors.initials: M
authors.lastname: Möller
authors.forename: Laura
authors.initials: L
authors.lastname: Saa
authors.forename: Ruta
authors.initials: R
authors.lastname: Grinyte
authors.forename: Valeri
authors.initials: V
authors.lastname: Pavlov
mesh_terms: D052157:Aptamers, Nucleotide / Q000032:analysis*; D001483:Base Sequence; D019187:Cadmium Compounds / Q000737:chemistry*; D017931:DNA Primers; D021881:DNA, Catalytic / Q000378:metabolism*; D018716:Molecular Mimicry; D015394:Molecular Structure; D049329:Nanostructures; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*; D013440:Sulfides / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D019187:Cadmium Compounds; D017931:DNA Primers; D021881:DNA, Catalytic; D013440:Sulfides; C034939:cadmium sulfide; D010544:Peroxidases