title: Single and combined effect of high-frequency loading and bisphosphonate treatment on the bone micro-architecture of ovariectomized rats.
abstract: Mechanical loading at high frequency affects bone. Whether this also applies to osteoporotic bone, combined or not with bisphosphonate therapy, was investigated in this animal study through imaging. An anabolic effect of high-frequency loading on osteoporotic bone, however non-synergistic with bisphosphonates, was found, thereby revealing its potential for treatment of osteoporosis.
  
  
  BACKGROUND
  In an effort to elucidate the effect of high-frequency (HF) loading on bone and to optimize its potential for treatment osteoporosis, this study aimed to investigate the effect of HF loading via whole body vibration (WBV), alone or in association with bisphosphonate treatment (alendronate--ALN), on the micro-architecture of ovariectomy (OVX)-induced compromised bone.
  
  
  METHODS
  Eighty-four female Wistar rats were ovariectomized (OVX) or sham-operated (shOVX). OVX animals were treated either with ALN (3 days/week at a dose of 2 mg/kg) or with saline solution. Each group (shOVX, OVX, ALN) was further divided into subgroups relative to the loading status (sham-WBV versus WBV) and the duration of experimental period (4 days versus 14 days). (Sham)WBV loading was applied for 10 min/day using 10 consecutive steps of HF loading (130, 135, 140, 145, 150, 130, 135, 140, 145, 150 Hz). Tibial bone structural responses to WBV and/or ALN treatment were analyzed using ex vivo micro-computed tomography.
  
  
  RESULTS
  The animal's hormonal status displayed a major impact on the trabecular and cortical bone structural parameters. Furthermore, mechanical treatment with HF WBV increased the cortical thickness and reduced the medullar area in OVX rats. However, OVX trabecular bone was not affected by HF stimuli. Finally, ALN prevented OVX-associated bone loss, but the association of ALN with WBV did not lead to a synergistic bone response in OVX bone.
  
  
  CONCLUSIONS
  HF WBV mechanical stimulation displayed an anabolic effect on osteoporotic cortical bone, confirming its therapeutic properties for enhancing compromised bone. Additionally, its association with bisphosphonates' administration did not produce any additive effect on the bone micro-architecture in the present study.
authors.affiliation: Department of Oral Health Sciences, BIOMAT Research Group, KU Leuven & University Hospitals Leuven, Leuven, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Hatori
authors.forename: G V
authors.initials: GV
authors.lastname: Camargos
authors.forename: M
authors.initials: M
authors.lastname: Chatterjee
authors.forename: F
authors.initials: F
authors.lastname: Faot
authors.forename: K
authors.initials: K
authors.lastname: Sasaki
authors.forename: J
authors.initials: J
authors.lastname: Duyck
authors.forename: K
authors.initials: K
authors.lastname: Vandamme
mesh_terms: D019386:Alendronate / Q000627:therapeutic use*; D000818:Animals; D050071:Bone Density Conservation Agents / Q000627:therapeutic use*; D003131:Combined Modality Therapy; D005260:Female; D006728:Hormones / Q000097:blood; D010024:Osteoporosis / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D010052:Ovariectomy; D017208:Rats, Wistar; D013977:Tibia / Q000000981:diagnostic imaging / Q000473:pathology; D014732:Vibration / Q000627:therapeutic use*; D015430:Weight Gain / Q000502:physiology; D016474:Weight-Bearing; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D050071:Bone Density Conservation Agents; D006728:Hormones; D019386:Alendronate