title: Serum fatty acid profiles and potential biomarkers of ankylosing spondylitis determined by gas chromatography-mass spectrometry and multivariate statistical analysis.
abstract: Ankylosing spondylitis (AS) is a common chronic inflammatory rheumatic disease. Early and accurate detection is essential for effective disease treatment. Recently, research has focused on genomics and proteomics. However, the associated metabolic variations, especially fatty acid profiles, have been poorly discussed. In this study, the gas chromatography-mass spectrometry (GC-MS) approach and multivariate statistical analysis were used to investigate the metabolic profiles of serum free fatty acids (FFAs) and esterified fatty acids (EFAs) in AS patients. The results showed that significant differences in most of the FFA (C12:0, C16:0, C16:1, C18:3, C20:4, C20:5, C22:5 and C22:6) and EFA (C12:0, C16:1, C18:0, C18:1, C18:2, C18:3, C20:4 and C22:6) concentrations were found between the AS patients and healthy controls (p < 0.05). Principal component analysis and partial least squares discriminant analysis were performed to classify the AS patients and controls. Additionally, FFAs C20:4, C12:0, C18:3 and EFAs C22:6, C12:0 were confirmed as potential biomarkers to identify AS patients and healthy controls. The present study highlights that differences in the serum FFA and EFA profiles of AS patients reflect the metabolic disorder. Moreover, FFA and EFA biomarkers appear to have clinical applications for the screening and diagnosis of AS.
authors.affiliation: Department of Spine Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.forename: Su
authors.initials: S
authors.lastname: Han
authors.forename: Daming
authors.initials: D
authors.lastname: Dong
authors.forename: Yansong
authors.initials: Y
authors.lastname: Wang
authors.forename: Qingpeng
authors.initials: Q
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Xie
authors.forename: Mi
authors.initials: M
authors.lastname: Li
authors.forename: Meng
authors.initials: M
authors.lastname: Yao
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008297:Male; D015999:Multivariate Analysis; D013167:Spondylitis, Ankylosing / Q000097:blood* / Q000175:diagnosis; D055815:Young Adult
chemical_list: D015415:Biomarkers; D005230:Fatty Acids, Nonesterified
keywords: GC-MS; ankylosing spondylitis; biomarker; esterified fatty acid; free fatty acid