title: Secretome analysis of the thermophilic xylanase hyper-producer Thermomyces lanuginosus SSBP cultivated on corn cobs.
abstract: Thermomyces lanuginosus is a thermophilic fungus known for its ability to produce industrially important enzymes including large amounts of xylanase, the key enzyme in hemicellulose hydrolysis. The secretome of T. lanuginosus SSBP was profiled by shotgun proteomics to elucidate important enzymes involved in hemicellulose saccharification and to characterise the presence of other industrially interesting enzymes. This study reproducibly identified a total of 74 proteins in the supernatant following growth on corn cobs. An analysis of proteins revealed nine glycoside hydrolase (GH) enzymes including xylanase GH11, β-xylosidase GH43, β-glucosidase GH3, α-galactosidase GH36 and trehalose hydrolase GH65. Two commercially produced Thermomyces enzymes, lipase and amylase, were also identified. In addition, other industrially relevant enzymes not currently explored in Thermomyces were identified including glutaminase, fructose-bisphosphate aldolase and cyanate hydratase. Overall, these data provide insight into the novel ability of a cellulase-free fungus to utilise lignocellulosic material, ultimately producing a number of enzymes important to various industrial processes.
authors.affiliation: Department of Biotechnology and Food Technology, Durban University of Technology, Durban, 4001, South Africa.
authors.forename: A M
authors.initials: AM
authors.lastname: Winger
authors.forename: J L
authors.initials: JL
authors.lastname: Heazlewood
authors.forename: L J G
authors.initials: LJ
authors.lastname: Chan
authors.forename: C J
authors.initials: CJ
authors.lastname: Petzold
authors.forename: K
authors.initials: K
authors.lastname: Permaul
authors.forename: S
authors.initials: S
authors.lastname: Singh
mesh_terms: D001203:Ascomycota / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D007218:Industrial Microbiology; D008031:Lignin / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D020543:Proteome / Q000378:metabolism; D014995:Xylosidases / Q000096:biosynthesis*; D003313:Zea mays
chemical_list: D011134:Polysaccharides; D020543:Proteome; C036909:lignocellulose; C007916:hemicellulose; D008031:Lignin; D006026:Glycoside Hydrolases; D014995:Xylosidases; C023305:hemicellulase; C026579:exo-1,4-beta-D-xylosidase