title: [Myogenic differentiation of laryngeal mucosal mesenchymal stem cells].
abstract: OBJECTIVE
  To investigate the myogenic differentiation of laryngeal mucosal mesenchymal stem cells (LM-MSCs) and the possibility of LM-MSCs as new alternative seed cells for laryngeal tissue engineering.
  
  
  METHODS
  LM-MSCs were separated from normal epiglottis mucosa and the cell surface markers including CD44, CD105, CD90, CD29, CD34 and CD45 were analyzed through flow cytometry. The osteogenesis and adipogenesis differentiation of LM-MSCs were investigated by oil red staining and alizarin red S staining. Immunofluorescence staining and RT-PCR were used to detect the expressions of myogenic differentiation markers including Myod1, Myogenin and myosin heavy chain (MyHc).
  
  
  RESULTS
  The separated LM-MSCs were in a fibrocyte-like form with long fusiform shape and grew adherent. The expression rates of cell surface markers LM-MSCs were CD44 (100.0%), CD105 (90.4%), CD90(99.9%), CD29 (93.0%), CD34 (0.4%) and CD45(1.3%) respectively. A number of beaded lipid drops and mineral deposition were observed after 14 days of adipogenesis differentiation and 21 days of osteogenesis differentiation. Myod1, Myogenin and MyHc genes appeared after 1 week and 3 weeks of myogenesis differentiation respectively.
  
  
  CONCLUSIONS
  The LM-MSCs have the properties of mesenchymal stem cells and could be differentiated into myoblasts, providing with the possibility to repair the damaged vocal cords with LM-MSCs through tissue engineering techniques.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
authors.forename: Runqin
authors.initials: R
authors.lastname: Yang
authors.forename: Leiguo
authors.initials: L
authors.lastname: Ming
authors.affiliation: Email: dengzh@fmmu.edu.cn.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Research and Development Center for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi 710032, China. Email yanjin@fmmu.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
mesh_terms: D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D004825:Epiglottis / Q000166:cytology; D006801:Humans; D007820:Laryngeal Mucosa / Q000166:cytology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D032446:Myoblasts / Q000166:cytology*