title: [Effect of low dosage of ciplatin on the shape and otoferlin in cochlea inner hair cells].
abstract: OBJECTIVE
  To establish the stable and efficient hearing damage model by using low dosage of cispla tin, and investigate the mechanism.
  
  
  METHODS
  C57 mice were divided into 7 groups (every group, n = 8), the first group was the control group, the others were separately intraperitoneally injected with different dosages of cispla tin for different time. We measured the auditory brainstem response (ABR) of the mice, and obtained the basal coil of organ Corti. We observed the shape of inner hair cells (IHC) by staining AgNO3 and marked otoferlin in the IHC by immunofluorescence,successively sliced by laser confocal microscopy. The RNA fragments were amplified by RT-PCR.
  
  
  RESULTS
  After cisplatin administration for four days, the thresholds of the ABR improved in 1.5 mg/kg and 3.0 mg/kg group, and compared with the control group, the ABR thresholds improved in each group with ciplatin administration for seven days. With the same dosage, the ABR threshold of the 0.75 mg/kg x 7 d group was higher than 0.75 mg/kg x 4 d group, and there was no time-effect relationship existing in other groups with different dosage. The otoferlin was less expressed 3.0 mg/kg groups than the control group. However, the oto ferlin expressed in the 1.5 mg/kg groups were almost the same as the control group. The alteration of the IHC was observed most remarkablely in 3.0 mg/kg x 7 d group.
  
  
  CONCLUSIONS
  Low dosage of cisplatin can impair the hearing, and the expression of otoferlin may involve in the underlying mechanism.
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Jiang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Yuan
mesh_terms: D000818:Animals; D002945:Cisplatin / Q000633:toxicity*; D003051:Cochlea / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006199:Hair Cells, Auditory, Inner / Q000187:drug effects / Q000473:pathology; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D008565:Membrane Proteins; C516233:otoferlin protein, mouse; D002945:Cisplatin