title: Evaluation of Borrelia real time PCR DNA targeting OspA, FlaB and 5S-23S IGS and Borrelia 16S rRNA RT-qPCR.
abstract: Borrelia burgdorferi non-sensu lato (s.l.) strains occurred in the Netherlands. A multiplex OspA, FlaB, IGS real time PCR was compared to 16S rRNA/rDNA RT-qPCR with lower average Cycle threshold (Ct) and LOD on strain dilutions. Multiplexing increased sensitivity on CSF samples (n=74), distinguishing B. burgdorferi s.l. from non-s.l. strains.
authors.affiliation: Department of Medical Microbiology, ASZ, Dordrecht, The Netherlands. Electronic address: B.de.Leeuw@RLM-microbiologie.nl.
authors.forename: Bertie H C G M
authors.initials: BH
authors.lastname: de Leeuw
authors.affiliation: Department of Medical Microbiology, ASZ, Dordrecht, The Netherlands.
authors.forename: Boulos
authors.initials: B
authors.lastname: Maraha
authors.affiliation: Department of Medical Microbiology, ASZ, Dordrecht, The Netherlands.
authors.forename: Leonie
authors.initials: L
authors.lastname: Hollemans
authors.affiliation: Laboratory for Zoonoses and Environmental Microbiology, RIVM, Bilthoven, The Netherlands.
authors.forename: Hein
authors.initials: H
authors.lastname: Sprong
authors.affiliation: Center for Infectious Diseases, Izore, Leeuwarden, The Netherlands.
authors.forename: Afke H
authors.initials: AH
authors.lastname: Brandenburg
authors.affiliation: Pathology Laboratory Dordrecht, PALdordt, Dordrecht, The Netherlands.
authors.forename: Pieter J
authors.initials: PJ
authors.lastname: Westenend
authors.affiliation: Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Johannes G
authors.initials: JG
authors.lastname: Kusters
mesh_terms: D000328:Adult; D000368:Aged; D000954:Antigens, Surface / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001428:Bacterial Vaccines / Q000235:genetics*; D001898:Borrelia / Q000145:classification / Q000235:genetics*; D021903:DNA, Ribosomal Spacer; D005260:Female; D005408:Flagellin / Q000235:genetics*; D006801:Humans; D008074:Lipoproteins / Q000235:genetics*; D008193:Lyme Disease / Q000175:diagnosis* / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D012335:RNA, Ribosomal; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D021903:DNA, Ribosomal Spacer; D008074:Lipoproteins; C072045:OspA protein; D012335:RNA, Ribosomal; D005408:Flagellin; C416532:flaB flagellin
keywords: Borrelia; Lyme; Real time PCR