title: Effect of storage temperature on the viability of human periodontal ligament fibroblasts.
abstract: BACKGROUND
  The viability of periodontal ligament fibroblasts (PDLF) can affect the long-term prognosis of replanted avulsed teeth. When immediate replantation of an avulsed tooth is not possible, the cells should be incubated in a physiological storage medium instantly to maintain their biological activity. The ability of different storage media to preserve PDLF viability has been previously evaluated. However, few studies have showed the effect of temperature on the viability of PDLF cultured with various storage media in vitro.
  
  
  METHODS
  This study was designed to measure PDLF activity by CCK-8 assay to compare the effectiveness at 4, 22 (room temperature), and 37°C under various storage media.
  
  
  RESULTS
  Statistical analysis demonstrated that tap water, saline, and saliva decreased cell viability as the storage temperature increased. But the temperature played only a minor role on cell viability when cells were incubated in Hank's balanced salt solution (HBSS), Dubelco's modified Eagle's medium (DMEM), or milk.
  
  
  CONCLUSIONS
  Within the parameters of this study, it seems that room temperature is adequate for storing the avulsed teeth in HBSS, DMEM, or milk in the extra-alveolar period.
authors.affiliation: Department of Stomatology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Fubo
authors.initials: F
authors.lastname: Chen
authors.forename: Shengcai
authors.initials: S
authors.lastname: Qi
authors.forename: Liyan
authors.initials: L
authors.lastname: Lu
authors.forename: Yuanzhi
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D007552:Isotonic Solutions / Q000494:pharmacology; D008892:Milk; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D010513:Periodontal Ligament / Q000166:cytology*; D012463:Saliva; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D014021:Tissue Preservation / Q000379:methods*; D014867:Water / Q000494:pharmacology
chemical_list: D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; D014867:Water; D012965:Sodium Chloride
keywords: CCK-8; PDLF; avulsed tooth; temperature