title: Study of the oipA genetic diversity and EPIYA motif patterns in cagA-positive Helicobacter pylori strains from Venezuelan patients with chronic gastritis.
abstract: CagA and OipA are involved, among other virulence factors, in the ability of Helicobacter pylori to colonize the gastric mucosa and to modulate the host environment during the establishment of chronic infection. The number and type of EPIYA phosphorylation motifs and the presence and functional status of oipA have been involved in the induction of cellular transformations playing an important role in the development of H. pylori associated gastric diseases. This work determined the prevalence of the oipA virulence factor and EPIYA motif patterns in cagA-positive H. pylori gastric biopsies from chronic gastritis patients from the Central-Western region of Venezuela. DNA was extracted directly from gastric biopsies collected by upper endoscopy from 113 patients. The EPIYA motif genotyping and oipA gene functional status was determined by PCR and sequencing. Phylogenetic analysis with the 3' variable region of cagA sequences was performed. Only Western-type EPIYA variants were detected: ABC (68.14%), ABCC (29.20%) and ABCCC (2.66%). High prevalence of strains with the oipA gene (93.8%) and its functional status "ON" (83%) was observed. No significant association between EPIYA motif patterns or oipA functional status with the histological changes in the gastric mucosa was found. Our study demonstrated the absolute predominance of the Western-type cagA gene in a Venezuelan admixed population. This is the first report showing oipA status of H. pylori strains in Venezuela. Further studies with a larger number of samples and including other pathologies are necessary to continue evaluating the role of the H. pylori virulence factors in the prevalence of gastric diseases in our country.
authors.affiliation: Laboratorio de Genética Molecular "Dr. Jorge Yunis-Turbay", Decanato de Ciencias de la Salud, Universidad Centroccidental Lisandro Alvarado (UCLA), Barquisimeto, Venezuela. Electronic address: keilatorres@ucla.edu.ve.
authors.forename: Keila
authors.initials: K
authors.lastname: Torres
authors.affiliation: Departamento de Anatomía Patológica, Hospital Antonio María Pineda-UCLA, Barquisimeto, Venezuela. Electronic address: elvisvalderrama@hotmail.com.
authors.forename: Elvis
authors.initials: E
authors.lastname: Valderrama
authors.affiliation: Centro de Biotecnología, Instituto de Estudios Avanzados, Caracas, Venezuela. Electronic address: marjo.say@gmail.com.
authors.forename: Marjorie
authors.initials: M
authors.lastname: Sayegh
authors.affiliation: Centro de Biotecnología, Instituto de Estudios Avanzados, Caracas, Venezuela. Electronic address: ramjoseluis@gmail.com.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Ramírez
authors.affiliation: Laboratorio de Genética Molecular "Dr. Jorge Yunis-Turbay", Decanato de Ciencias de la Salud, Universidad Centroccidental Lisandro Alvarado (UCLA), Barquisimeto, Venezuela. Electronic address: mchiurillo@ucla.edu.ve.
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Chiurillo
mesh_terms: D020816:Amino Acid Motifs; D000942:Antigens, Bacterial / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001706:Biopsy; D002908:Chronic Disease; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D005756:Gastritis / Q000382:microbiology*; D014644:Genetic Variation; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006651:Histocytochemistry; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014687:Venezuela
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C526444:OipA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori
keywords: Chronic gastritis; EPIYA; Helicobacter pylori; Venezuela; cagA; oipA