title: Control of predators in industrial scale microalgae cultures with Pulsed Electric Fields.
abstract: This work describes the utilization of Pulsed Electric Fields to control the protozoan contamination of a microalgae culture, in an industrial 2.7 m(3) microalgae photobioreactor. The contaminated culture was treated with Pulsed Electric Fields, PEF, for 6h with an average of 900 V/cm, 65 μs pulses of 50 Hz. Working with recirculation, all the culture was uniformly exposed to the PEF throughout the assay. The development of the microalgae and protozoan populations was followed and the results showed that PEF is effective on the selective elimination of protozoa from microalgae cultures, inflicting on the protozoa growth halt, death or cell rupture, without affecting microalgae productivity. Specifically, the results show a reduction of the active protozoan population of 87% after 6h treatment and 100% after few days of normal cultivation regime. At the same time, microalgae growth rate remained unaffected.
authors.affiliation: A4F-Algafuel S.A., Lisbon, Portugal.
authors.forename: D
authors.initials: D
authors.lastname: Rego
authors.affiliation: Lisbon Engineering Superior Institute, ISEL, Lisbon, Portugal. Electronic address: lmredondo@deea.isel.ipl.pt.
authors.forename: L M
authors.initials: LM
authors.lastname: Redondo
authors.affiliation: A4F-Algafuel S.A., Lisbon, Portugal.
authors.forename: V
authors.initials: V
authors.lastname: Geraldes
authors.affiliation: A4F-Algafuel S.A., Lisbon, Portugal.
authors.forename: L
authors.initials: L
authors.lastname: Costa
authors.affiliation: A4F-Algafuel S.A., Lisbon, Portugal.
authors.forename: J
authors.initials: J
authors.lastname: Navalho
authors.affiliation: EnergyPulse Systems, EPS, Lisbon, Portugal.
authors.forename: M T
authors.initials: MT
authors.lastname: Pereira
mesh_terms: D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D002462:Cell Membrane; D055664:Electrochemical Techniques / Q000379:methods*; D004867:Equipment Design; D058086:Microalgae / Q000254:growth & development* / Q000469:parasitology*; D058433:Photobioreactors
keywords: Industrial photobioreactor; Microalgae; Pulsed Electric Fields