title: Cloning and expression analysis of a peptidoglycan recognition protein in silkworm related to virus infection.
abstract: In this study, the full-length cDNA of a peptidoglycan recognition protein named BmPGRP-S3 was identified from the silkworm, Bombyx mori by rapid amplification of cDNA ends. It is 807 bp and comprises the following: a 5'-untranslated region (UTR) with a length of 112 bp, a 3'-UTR with a length of 92 bp including a poly-adenylation signal sequence (AATAAA) and a poly(A) tail. The longest open reading frame (ORF) of BmPGRP-S3 is 603 bp and encodes a polypeptide of 200 amino acids with a predicted molecular weight of 22.3 kDa including a PGRP domain. Sequence similarity and phylogenic analysis results indicated that BmPGRP-S3 belongs to the group of insect PGRPs and is closer to BmPGRP-S4 with the highest identity of 68%. Fluorescent quantitative real-time PCR results revealed that the mRNA transcripts of BmPGRP-S3 were presented in all of the tissues, but were highest in the midgut. In the silkworm larvae infected with B. mori cytoplasmic polyhedrosis virus (BmCPV), the relative expression level of BmPGRP-S3 was upregulated. The DNA segment of a mature BmPGRP-S3 peptide was inserted into the expression plasmid pET-28a(+) to construct a recombinant expression plasmid. Western blot results revealed that mature BmPGRP-S3 could be detected in the hemolymph and midgut which were the most important immune tissues in silkworm. All the results suggested that BmPGRP-S3 may play an important role in the immune response of silkworm to BmCPV infection and provided helpful information for further studying the function of BmPGRP-S3 in silkworm.
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu, China; College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212003, Jiangsu, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Gao
authors.affiliation: College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212003, Jiangsu, China.
authors.forename: Xiang-Yuan
authors.initials: XY
authors.lastname: Deng
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu, China.
authors.forename: He-Ying
authors.initials: HY
authors.lastname: Qian
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu, China.
authors.forename: Guang-Xing
authors.initials: GX
authors.lastname: Qin
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu, China.
authors.forename: Cheng-Xiang
authors.initials: CX
authors.lastname: Hou
authors.affiliation: Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu, China. Electronic address: guoxijie@126.com.
authors.forename: Xi-Jie
authors.initials: XJ
authors.lastname: Guo
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics* / Q000821:virology; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics; D019476:Insect Proteins / Q000235:genetics*; D007814:Larva / Q000235:genetics / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D012087:Reoviridae / Q000235:genetics*; D012088:Reoviridae Infections / Q000235:genetics* / Q000821:virology; D017422:Sequence Analysis, DNA
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D002352:Carrier Proteins; D018076:DNA, Complementary; D019476:Insect Proteins; D012333:RNA, Messenger; C118897:peptidoglycan recognition protein
keywords: Bombyx mori; Cytoplasmic polyhedrosis virus; Peptidoglycan recognition protein; Protein expression