title: Evaluation of Phytochemical Screening and Anti Inflammatory Activity of Leaves and Stem of Mikania scandens (L.) Wild.
abstract: BACKGROUND
  The greatest disadvantage in the presently available potent synthetic anti-inflammatory drugs lies in their toxicity and reappearance of symptoms after discontinuation. Hence, people are returning to the natural products with the hope of safety and security. Several species of Mikania have been reported to have anti-inflammatory properties.
  
  
  OBJECTIVE
  The present study aims to assess the anti-inflammatory activity of the ethanolic extract of the leaves and stem of Mikania scandens in vivo and in vitro.
  
  
  METHODS
  The in vitro bioassay consisted of assaying the effect of the extracts against denaturation of protein (egg albumin) and measuring the absorbance. In vivo anti-inflammatory activity was checked by measuring the percentage inhibition of carrageenan-induced rat paw edema after oral administration of the extracts to male Wistar rats.
  
  
  RESULTS
  The plant extracts revealed the presence of tannins, alkaloids, steroids and flavonoids in both the leaf and stem extracts. The in vitro study of leaf extracts of M. scandens demonstrated that at 16000 μg/ml concentration a better anti-inflammatory activity was exhibited which is more than the stem extracts. Similarly in the in vivo study, carrageenan induced inflammation was significantly antagonized by M. scandens leaf extract, with inhibition of 50% at 1000 mg/kg.
  
  
  CONCLUSIONS
  The ethanolic extract of both leaf and stem of M. scandens showed potent anti-inflammatory activity. In comparison the leaf extract found to be more potent in both the conditions in vivo and in vitro, comparing with the standard drug diclofenac sodium and traditional control rumalaya perhaps due to the presence of phytochemicals like alkaloids and flavonoids in the plant.
authors.affiliation: Department of Biotechnology, Heritage Institute of Technology, Anandapur, Kolkata, West Bengal, India.
authors.forename: S
authors.initials: S
authors.lastname: Banerjee
authors.affiliation: Department of Biotechnology, Heritage Institute of Technology, Anandapur, Kolkata, West Bengal, India.
authors.forename: A
authors.initials: A
authors.lastname: Chanda
authors.affiliation: Department of Pharmacology, R.G. Kar Medical College and Hospital, Kolkata, West Bengal, India.
authors.forename: A
authors.initials: A
authors.lastname: Adhikari
authors.affiliation: Department of Pharmacology, R.G. Kar Medical College and Hospital, Kolkata, West Bengal, India.
authors.forename: Ak
authors.initials: A
authors.lastname: Das
authors.affiliation: Department of Anatomy, Nilratan Sircar Medical College, Kolkata, West Bengal, India.
authors.forename: S
authors.initials: S
authors.lastname: Biswas
keywords: Anti-inflammatory; Mikania scandens (L.) wild; Phytochemicals; Protein denaturation assay; Rat paw edema