title: Evidence of genotoxicity in lymphocytes of non-smoking alcoholics.
abstract: Alcohol abuse is a significant public health issue. Epidemiological studies conducted on different populations consistently showed that consumption of alcoholic beverages is associated with cytogenetic damages and higher risk for several types of cancer. However, the interpretation of many cytogenetic studies resulted complicated because some confounding factors, such as smoking habit, are not always taken into account. In the present study, the frequency of sister chromatid exchanges (SCEs), chromosome aberrations (CAs) and micronuclei (MNs) in cultured human lymphocytes was assessed on 15 alcoholic and 15 non-alcoholic control male subjects. Moreover, considering the implication of the Glutathione S-transferases gene polymorphisms in the genetic susceptibility to alcoholic liver diseases, we considered an important issue to evaluate the relationship between these gene polymorphisms and the cytogenetic damage. In our sample we exclusively considered individuals that did not smoke nor consume drugs for a period of at least 2 years prior to the analysis. Statistically significant differences were found between alcoholics and controls in the frequency of SCEs/cell (P = 0.001), RI value (P = 0.001), CAs (P = 0.002) and CAB (P = 0.002). Vice versa, no significant differences were found between alcoholics and controls in terms of MNs frequency and CBPI value. In both samples, no statistically significant association was found between the analysed GSTs gene polymorphisms and the frequencies of MNs, SCEs and CAs. Finally, among alcoholics we found a positive correlation between SCEs and CAs frequencies and the duration of alcohol abuse.
authors.affiliation: Department of Life Sciences and Systems Biology, University of Turin, Via Accademia Albertina n. 13, 10123, Turin, Italy, alfredo.santovito@unito.it.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Santovito
authors.forename: Piero
authors.initials: P
authors.lastname: Cervella
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Delpero
mesh_terms: D000328:Adult; D000368:Aged; D057229:Alcoholics; D000437:Alcoholism / Q000473:pathology*; D016022:Case-Control Studies; D049109:Cell Proliferation; D002869:Chromosome Aberrations; D048749:Cytokinesis; D004249:DNA Damage; D003710:Demography; D006801:Humans; D008214:Lymphocytes / Q000473:pathology*; D008297:Male; D008677:Metaphase; D048631:Micronucleus, Germline; D008875:Middle Aged; D012044:Regression Analysis; D012854:Sister Chromatid Exchange; D012907:Smoking / Q000009:adverse effects*