title: Effect of retinal ischemia on the non-image forming visual system.
abstract: Retinal ischemic injury is an important cause of visual impairment. The loss of retinal ganglion cells (RGCs) is a key sign of retinal ischemic damage. A subset of RGCs expressing the photopigment melanopsin (mRGCs) regulates non-image-forming visual functions such as the pupillary light reflex (PLR), and circadian rhythms. We studied the effect of retinal ischemia on mRGCs and the non-image-forming visual system function. For this purpose, transient ischemia was induced by raising intraocular pressure to 120 mm Hg for 40 min followed by retinal reperfusion by restoring normal pressure. At 4 weeks post-treatment, animals were subjected to electroretinography and histological analysis. Ischemia induced a significant retinal dysfunction and histological alterations. At this time point, a significant decrease in the number of Brn3a(+) RGCs and in the anterograde transport from the retina to the superior colliculus and lateral geniculate nucleus was observed, whereas no differences in the number of mRGCs, melanopsin levels, and retinal projections to the suprachiasmatic nuclei and the olivary pretectal nucleus were detected. At low light intensity, a decrease in pupil constriction was observed in intact eyes contralateral to ischemic eyes, whereas at high light intensity, retinal ischemia did not affect the consensual PLR. Animals with ischemia in both eyes showed a conserved locomotor activity rhythm and a photoentrainment rate which did not differ from control animals. These results suggest that the non-image forming visual system was protected against retinal ischemic damage.
authors.affiliation: Laboratorio de Neuroquímica Retiniana y Oftalmología Experimental, Departamento de Bioquímica Humana, Facultad de Medicina/CEFyBO, Universidad de Buenos Aires/CONICET , Buenos Aires , Argentina.
authors.forename: María Florencia
authors.initials: MF
authors.lastname: González Fleitas
authors.forename: Melina
authors.initials: M
authors.lastname: Bordone
authors.forename: Ruth E
authors.initials: RE
authors.lastname: Rosenstein
authors.forename: Damián
authors.initials: D
authors.lastname: Dorfman
mesh_terms: D000818:Animals; D002772:Cholera Toxin / Q000737:chemistry; D002940:Circadian Rhythm / Q000502:physiology; D004596:Electroretinography; D005829:Geniculate Bodies / Q000473:pathology; D007511:Ischemia / Q000473:pathology*; D008027:Light; D008297:Male; D009068:Movement; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000473:pathology / Q000503:physiopathology*; D012165:Retinal Ganglion Cells / Q000378:metabolism; D017299:Rod Opsins / Q000378:metabolism; D013493:Suprachiasmatic Nucleus / Q000378:metabolism; D013997:Time Factors; D014785:Vision, Ocular; D000099210:Melanopsin
chemical_list: D002772:Cholera Toxin; D017299:Rod Opsins; D000099210:Melanopsin
keywords: Ischemia; melanopsin; non-image forming visual system; retina