title: Sphingorhabdus pacificus sp. nov., isolated from sandy sediments of the Sea of Japan seashore.
abstract: An aerobic, Gram-negative, yellow-pigmented, non-motile rod-shaped bacterium designated KMM 9574(T) was isolated from a sand sediment sample collected from the Sea of Japan seashore. Comparative 16S rRNA gene sequence analysis showed that strain KMM 9574(T) belonged to the genus Sphingorhabdus sharing a highest sequence similarity to Sphingorhabdus marina JCM 14161(T) 96.8 %. Strain KMM 9574(T) was characterized by the major ubiquinone Q-10, and by the predominance of C(18:1) ω7c, C(16:0) 2-OH, C(16:1) ω7c, C(17:1), followed by C(15:0) 2-OH and C(14:0) 2-OH in its fatty acid profile. Polar lipids consisted of phosphatidylcholine, sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unknown phospholipid, and an unknown lipid. The DNA G+C content was 56.5 mol %. Based on phylogenetic analysis and distinctive phenotypic characteristics, strain 9574(T) is concluded to represent a novel species of the genus Sphingorhabdus, for which the name Sphingorhabdus pacificus sp. nov., is proposed. The type strain of the species is strain KMM 9574(T) (= NRIC 0922(T) = JCM 30177(T)).
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, 690022, Vladivostok, Russia, lro@piboc.dvo.ru.
authors.forename: Lyudmila A
authors.initials: LA
authors.lastname: Romanenko
authors.forename: Naoto
authors.initials: N
authors.lastname: Tanaka
authors.forename: Vassilii I
authors.initials: VI
authors.lastname: Svetashev
authors.forename: Valery V
authors.initials: VV
authors.lastname: Mikhailov
mesh_terms: D001482:Base Composition; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D013045:Species Specificity; D042301:Sphingomonadaceae / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D005227:Fatty Acids; D008055:Lipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S