title: Structural changes of ultrasonicated bovine serum albumin revealed by hydrogen-deuterium exchange and mass spectrometry.
abstract: The structural changes of bovine serum albumin (BSA) under high-intensity ultrasonication were investigated by fluorescence spectroscopy and mass spectrometry. Evidence for the ultrasonication-induced conformational changes of BSA was provided by the intensity changes and maximum-wavelength shift in fluorescence spectrometry. Matrix-assisted laser desorption-ionization time-of-flight mass spectroscopy (MALDI-TOF MS) revealed the increased intensity of the peak at the charge state +5 and a newly emerged peak at charge state +6, indicating that the protein became unfolded after ultrasonication. Prevalent unfolding of BSA after ultrasonication was revealed by hydrogen-deuterium exchange coupled with mass spectrometry (HDX-MS). Increased intensity and duration of ultrasonication further promoted the unfolding of the protein. The unfolding induced by ultrasonication goes through an intermediate state similar to that induced by a low concentration of denaturant.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, 330047, China.
authors.forename: Qiuting
authors.initials: Q
authors.lastname: Zhang
authors.forename: Zongcai
authors.initials: Z
authors.lastname: Tu
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Huang
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Sha
authors.forename: Hui
authors.initials: H
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002417:Cattle; D003903:Deuterium / Q000737:chemistry*; D041961:Deuterium Exchange Measurement / Q000379:methods*; D006859:Hydrogen / Q000737:chemistry*; D011487:Protein Conformation / Q000528:radiation effects*; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000528:radiation effects*; D013050:Spectrometry, Fluorescence; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014465:Ultrasonics
chemical_list: D012710:Serum Albumin, Bovine; D006859:Hydrogen; D003903:Deuterium