title: Novel isolation strategy to deliver pure fetal-origin and maternal-origin mesenchymal stem cell (MSC) populations from human term placenta.
abstract: The placenta is an abundant source of mesenchymal stem/stromal cells (MSC). Although presumed of translationally-advantageous fetal origin, the literature instead suggests a high incidence of either contaminating or pure maternal MSC. Despite definitional criteria that MSC are CD34-, increasing evidence suggests that fetal MSC may be CD34 positive in vivo. We flow sorted term placental digests based on CD34+ expression and exploited differential culture media to isolate separately pure fetal and maternal MSC populations. This method has considerable translational implications, in particular to clinical trials underway with "placental" MSC of uncertain or decidual origin.
authors.affiliation: The University of Queensland, UQ Centre for Clinical Research, Herston, QLD, 4029, Australia. Electronic address: j.patel@uq.edu.au.
authors.forename: J
authors.initials: J
authors.lastname: Patel
authors.affiliation: The University of Queensland, UQ Centre for Clinical Research, Herston, QLD, 4029, Australia.
authors.forename: A
authors.initials: A
authors.lastname: Shafiee
authors.affiliation: The University of Queensland, UQ Centre for Clinical Research, Herston, QLD, 4029, Australia.
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.affiliation: The University of Queensland, UQ Centre for Clinical Research, Herston, QLD, 4029, Australia; Centre for Advanced Prenatal Care, Women's & Newborn Services, Royal Brisbane & Women's Hospital, Herston, QLD, 4029, Australia.
authors.forename: N M
authors.initials: NM
authors.lastname: Fisk
authors.affiliation: The University of Queensland, UQ Centre for Clinical Research, Herston, QLD, 4029, Australia; The University of Queensland, UQ Diamantina Institute, Translational Research Institute, Woolloongabba, QLD, 4102, Australia. Electronic address: k.khosrotehrani@uq.edu.au.
authors.forename: K
authors.initials: K
authors.lastname: Khosrotehrani
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis; D002469:Cell Separation / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy
chemical_list: D018952:Antigens, CD34
keywords: Chorion; Decidua; Fetal; Maternal; Mesenchymal stem cells; Placenta