title: Serum miRNA profiling identifies miR-150/30a as potential biomarker for workers with damaged nerve fibers from carbon disulfide.
abstract: As crucial small regulatory molecules, serum microRNAs (miRNAs) have been widely identified as potential noninvasive biomarkers. To survey and identify serum miRNAs associated with workers who had experienced injury to their nerve system from carbon disulfide (CS2), we profiled abnormally expressed miRNAs using the microarray technique and further performed qRT-PCR validation in case and control samples (n=20). Microarray profiling in pooled RNA samples showed that many miRNAs in workers exposed to CS2 were aberrantly expressed. Based on control samples exposed to CS2, a great amount of abnormal miRNAs, including some miRNA gene clusters and families, were obtained from microarray datasets. Most of deregulated miRNAs were up-regulated, and almost all miRNAs showed consistent expression patterns between workers with different numbers of damaged nerve fibers. Functional enrichment analysis suggested that these abnormal miRNAs showed versatile roles by contributing to multiple biological processes. Some aberrantly expressed miRNAs were characterized as miRNA gene clusters or families, and they always showed consistent expression patterns. miR-150 and miR-30a were selected to be further validated by qRT-PCR as up-regulated species, and they could discern case samples from control samples. miR-150 and miR-30a may be potential noninvasive biomarkers for a damaged nervous system.
authors.affiliation: Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, China.
authors.forename: Li
authors.initials: L
authors.lastname: Guo
authors.forename: Chen
authors.initials: C
authors.lastname: Luo
authors.forename: Jingjing
authors.initials: J
authors.lastname: Fan
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Hou
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Ji
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Baoli
authors.initials: B
authors.lastname: Zhu
authors.forename: Chunhui
authors.initials: C
authors.lastname: Ni
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D002246:Carbon Disulfide / Q000633:toxicity*; D016022:Case-Control Studies; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D009412:Nerve Fibers / Q000187:drug effects* / Q000473:pathology; D009422:Nervous System Diseases / Q000097:blood* / Q000139:chemically induced; D016273:Occupational Exposure / Q000009:adverse effects*; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D015415:Biomarkers; D002246:Carbon Disulfide; D035683:MicroRNAs; C533097:MIR150, human; C546086:MIRN30b microRNA, human