title: A comparative proteomic analysis of Bacillus coagulans in response to lactate stress during the production of L-lactic acid.
abstract: The growth rate and maximum biomass of Bacillus coagulans 2-6 were inhibited by lactate; inhibition by sodium lactate was stronger than by calcium lactate. The differences of protein expressions by B. coagulans 2-6 under the lactate stress were determined using two-dimensional electrophoresis coupled with mass spectrometric identification. Under the non-stress condition, calcium lactate stress and sodium lactate stress, the number of detected protein spots was 1,571 ± 117, 1,281 ± 231 and 904 ± 127, respectively. Four proteins with high expression under lactate stress were identified: lactate dehydrogenase, cysteine synthase A, aldo/keto reductase and ribosomal protein L7/L12. These proteins are thus potential targets for the reconstruction of B. coagulans to promote its resistance to lactate stress.
authors.affiliation: College of Pharmacy, Binzhou Medical University, Yantai, 264003, People's Republic of China, wangxiuwen0822@163.com.
authors.forename: Xiuwen
authors.initials: X
authors.lastname: Wang
authors.forename: Jiayang
authors.initials: J
authors.lastname: Qin
authors.forename: Landong
authors.initials: L
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
mesh_terms: D001407:Bacillus / Q000737:chemistry* / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional; D006131:Growth Inhibitors / Q000378:metabolism / Q000633:toxicity; D007773:Lactates / Q000378:metabolism* / Q000633:toxicity; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis* / Q000187:drug effects*; D040901:Proteomics; D013312:Stress, Physiological
chemical_list: D006131:Growth Inhibitors; D007773:Lactates; D020543:Proteome