title: Synthesis, interaction with DNA and antiproliferative activities of two novel Cu(II) complexes with norcantharidin and benzimidazole derivatives.
abstract: Two novel complexes [Cu(L)2(Ac)2]·3H2O (1) (L=N-2-methyl benzimidazole demethylcantharate imide, C16H15N3O3, Ac=acetate, C2H3O2) and [Cu(bimz)2(DCA)] (2) (bimz=benzimidazole, C7H6N2; DCA=demethylcantharate, C8H8O5) were synthesized and characterized by elemental analysis, infrared spectra and X-ray diffraction techniques. Cu(II) ion was four-coordinated in complex 1, Cu(II) ion was five-coordinated in complex 2. A large amount of intermolecular hydrogen-bonding and π-π stacking interactions were observed in these complex structures. The DNA-binding properties of these complexes were investigated using electronic absorption spectra, fluorescence spectra, viscosity measurements and agarose gel electrophoresis. The interactions between the complexes and bovine serum albumin (BSA) were investigated by fluorescence spectra. The antiproliferative activities of the complexes against human hepatoma cells (SMMC7721) were tested in vitro. And the results showed that these complexes could bind to DNA in moderate intensity via partial intercalation, and complexes 1 and 2 could cleave plasmid DNA through hydroxyl radical mechanism. Title complexes could effectively quench the fluorescence of BSA through static quenching. Meanwhile, title complexes had stronger antiproliferative effect compared to L and Na2(DCA) within the tested concentration range. And complex 1 possessed more antiproliferative active than complex 2.
authors.affiliation: Zhejiang Key Laboratory for Reactive Chemistry on Solid Surfaces, Zhejiang Normal University, 321004, PR China; College of Chemical and Life Science, Zhejiang Normal University, 321004, PR China.
authors.forename: Wen-Ji
authors.initials: WJ
authors.lastname: Song
authors.affiliation: Zhejiang Key Laboratory for Reactive Chemistry on Solid Surfaces, Zhejiang Normal University, 321004, PR China; College of Chemical and Life Science, Zhejiang Normal University, 321004, PR China. Electronic address: sky51@zjnu.cn.
authors.forename: Qiu-Yue
authors.initials: QY
authors.lastname: Lin
authors.affiliation: College of Chemical and Life Science, Zhejiang Normal University, 321004, PR China.
authors.forename: Wen-Jiao
authors.initials: WJ
authors.lastname: Jiang
authors.affiliation: College of Chemical and Life Science, Zhejiang Normal University, 321004, PR China.
authors.forename: Fang-Yuan
authors.initials: FY
authors.lastname: Du
authors.affiliation: College of Chemical and Life Science, Zhejiang Normal University, 321004, PR China. Electronic address: Qingyuanqi@zjnu.cn.
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Qi
authors.affiliation: College of Chemical and Life Science, Zhejiang Normal University, 321004, PR China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wei
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D001562:Benzimidazoles / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000378:metabolism; D002417:Cattle; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D003300:Copper / Q000737:chemistry* / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism; D006801:Humans; D007364:Intercalating Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D008113:Liver Neoplasms / Q000188:drug therapy / Q000378:metabolism; D008958:Models, Molecular; D012710:Serum Albumin, Bovine / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D001562:Benzimidazoles; D019086:Bridged Bicyclo Compounds, Heterocyclic; D056831:Coordination Complexes; D007364:Intercalating Agents; D012710:Serum Albumin, Bovine; D003300:Copper; C069741:norcantharidin; D004247:DNA; C031000:benzimidazole
keywords: Antiproliferative activity; Benzimidazole derivatives; Copper complex; DNA binding; Norcantharidin