title: Integrated microcapillary for sample-to-answer nucleic acid pretreatment, amplification, and detection.
abstract: This work develops an integrated microcapillary-based loop-mediated isothermal amplification (icLAMP) containing preloaded reagents and DNA extraction card, allowing for sample-to-answer screening of single nucleotide polymorphisms (SNPs) typing of the CYP2C19 gene from untreated blood samples with minimal user operation. With all reagents and the DNA extraction card preloaded inside the capillary, this icLAMP system can achieve on-site pretreatment, extraction, amplification, and detection of nucleic acids within 150 min, without the requirement for advanced instruments. As icLAMP technology carries many advantages such as disposability, easy operation, low cost, and reduced cross contamination and biohazard risks, we expect this system to have a great impact on point-of-care (POC) nucleic acid detection.
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology , Beijing 100190, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Feng
authors.forename: Fei
authors.initials: F
authors.lastname: Fan
authors.forename: Guojun
authors.initials: G
authors.lastname: Zhang
authors.forename: Xixiong
authors.initials: X
authors.lastname: Kang
authors.forename: Xuzhen
authors.initials: X
authors.lastname: Qin
authors.forename: Jiashu
authors.initials: J
authors.lastname: Sun
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Li
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods*; D005820:Genetic Testing; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation*; D009696:Nucleic Acids / Q000032:analysis / Q000737:chemistry*; D013997:Time Factors
chemical_list: D009696:Nucleic Acids