title: Detection of cyclic di-AMP using a competitive ELISA with a unique pneumococcal cyclic di-AMP binding protein.
abstract: Cyclic di-AMP (c-di-AMP) is a recently recognized bacterial signaling molecule. In this study, a competitive enzyme-linked immunosorbent assay (ELISA) for the quantification of c-di-AMP was developed using a novel pneumococcal c-di-AMP binding protein (CabP). With this method, c-di-AMP concentrations in biological samples can be quickly and accurately quantified.
authors.affiliation: Center for Immunology and Microbial Disease, MC-151, Albany Medical College, Albany, NY 12208-3479, USA.
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Underwood
authors.affiliation: Center for Immunology and Microbial Disease, MC-151, Albany Medical College, Albany, NY 12208-3479, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Center for Immunology and Microbial Disease, MC-151, Albany Medical College, Albany, NY 12208-3479, USA.
authors.forename: Dennis W
authors.initials: DW
authors.lastname: Metzger
authors.affiliation: Center for Immunology and Microbial Disease, MC-151, Albany Medical College, Albany, NY 12208-3479, USA. Electronic address: baig@mail.amc.edu.
authors.forename: Guangchun
authors.initials: G
authors.lastname: Bai
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002352:Carrier Proteins; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D011485:Protein Binding; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013296:Streptococcus pneumoniae / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D000242:Cyclic AMP
keywords: Detection; ELISA; c-di-AMP; c-di-AMP binding protein