title: Tumor-targeting Salmonella typhimurium A1-R prevents experimental human breast cancer bone metastasis in nude mice.
abstract: Bone metastasis is a lethal and morbid late stage of breast cancer that is currently treatment resistant. More effective mouse models and treatment are necessary. High bone-metastatic variants of human breast cancer cells were selected in nude mice by cardiac injection. After cardiac injection of a high bone-metastatic variant of breast cancer, all untreated mice had bone metastases compared to only 20% with parental cells. Treatment with tumor-targeting Salmonella typhimurium A1-R completely prevented the appearance of bone metastasis of the high metastatic variant in nude mice (P < 0.001). After injection of the highly bone-metastatic breast cancer variant to the tibia of nude mice, S. typhimurium A1-R treatment significantly reduced tumor growth in the bone (P < 0.001). These data indicated that S. typhimurium A1-R is useful to prevent and inhibit breast cancer bone metastasis and should be of future clinical use for breast cancer in the adjuvant setting.
authors.affiliation: AntiCancer, Inc.; San Diego, California USA. Department of Surgery; University of California, San Diego; San Diego, California USA. Department of Orthopedic Surgery; Kanazawa University Graduate School of Medical Sciences; Kanazawa, Ishikawa, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Miwa
authors.affiliation: AntiCancer, Inc.; San Diego, California USA. Department of Surgery; University of California, San Diego; San Diego, California USA.
authors.forename: Shuya
authors.initials: S
authors.lastname: Yano
authors.affiliation: AntiCancer, Inc.; San Diego, California USA. Department of Surgery; University of California, San Diego; San Diego, California USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Surgery; University of California, San Diego; San Diego, California USA.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Matsumoto
authors.affiliation: AntiCancer, Inc.; San Diego, California USA. Department of Surgery; University of California, San Diego; San Diego, California USA.
authors.forename: Fuminari
authors.initials: F
authors.lastname: Uehara
authors.affiliation: AntiCancer, Inc.; San Diego, California USA. Department of Surgery; University of California, San Diego; San Diego, California USA.
authors.forename: Mako
authors.initials: M
authors.lastname: Yamamoto
authors.affiliation: AntiCancer, Inc.; San Diego, California USA. Department of Surgery; University of California, San Diego; San Diego, California USA.
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Hiroshima
authors.affiliation: Department of Orthopedic Surgery; Kanazawa University Graduate School of Medical Sciences; Kanazawa, Ishikawa, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kimura
authors.affiliation: Department of Orthopedic Surgery; Kanazawa University Graduate School of Medical Sciences; Kanazawa, Ishikawa, Japan.
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Hayashi
authors.affiliation: Department of Orthopedic Surgery; Kanazawa University Graduate School of Medical Sciences; Kanazawa, Ishikawa, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Yamamoto
authors.affiliation: Department of Surgery; University of California, San Diego; San Diego, California USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Bouvet
authors.affiliation: Department of Orthopedic Surgery; Kanazawa University Graduate School of Medical Sciences; Kanazawa, Ishikawa, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsuchiya
authors.affiliation: AntiCancer, Inc.; San Diego, California USA. Department of Surgery; University of California, San Diego; San Diego, California USA.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
authors.affiliation: AntiCancer, Inc.; San Diego, California USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000382:microbiology / Q000517:prevention & control* / Q000556:secondary*; D001943:Breast Neoplasms / Q000382:microbiology* / Q000473:pathology / Q000628:therapy*; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D012486:Salmonella typhimurium; D023041:Xenograft Model Antitumor Assays