title: Microsatellite primers for the gynodioecious grassland perennial Saxifraga granulata (Saxifragaceae).
abstract: OBJECTIVE
  Nine polymorphic and 12 monomorphic microsatellite loci (simple sequence repeats [SSRs]) were isolated and characterized for the gynodioecious grassland perennial Saxifraga granulata. •
  
  
  RESULTS
  Based on genomic screening of leaf material of four individuals from four populations, a total of 21 microsatellite primer pairs were designed for S. granulata. Nine loci were polymorphic and were optimized into two PCR multiplex reactions and tested on 100 individuals from five riparian populations from central Belgium. The number of alleles of the polymorphic loci ranged from three to 18, and gametic heterozygosity ranged from 0.26 to 0.94. •
  
  
  CONCLUSIONS
  The markers that are presented here are the first microsatellite markers reported for S. granulata and will be used to assess how river systems shape the spatial genetic structure and diversity of riparian populations of this species.
authors.affiliation: Laboratory of Plant Conservation and Population Biology, KU Leuven-University of Leuven, Kasteelpark Arenberg 31, B-3001 Heverlee, Belgium.
authors.forename: Sascha
authors.initials: S
authors.lastname: van der Meer
authors.affiliation: Laboratory of Biodiversity and Evolutionary Genomics, KU Leuven-University of Leuven, Charles Deberiotstraat 32, B-3000 Leuven, Belgium.
authors.forename: Jeroen K J
authors.initials: JK
authors.lastname: Van Houdt
authors.affiliation: Laboratory of Biodiversity and Evolutionary Genomics, KU Leuven-University of Leuven, Charles Deberiotstraat 32, B-3000 Leuven, Belgium ; Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Townsville, 4811 Queensland, Australia.
authors.forename: Gregory E
authors.initials: GE
authors.lastname: Maes
authors.affiliation: Laboratory of Biodiversity and Evolutionary Genomics, KU Leuven-University of Leuven, Charles Deberiotstraat 32, B-3000 Leuven, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: Hellemans
authors.affiliation: Laboratory of Plant Conservation and Population Biology, KU Leuven-University of Leuven, Kasteelpark Arenberg 31, B-3001 Heverlee, Belgium.
authors.forename: Hans
authors.initials: H
authors.lastname: Jacquemyn
keywords: Saxifraga granulata; Saxifragaceae; gynodioecy; heterozygosity; polyploidy; simple sequence repeat (SSR)