title: Identification of a novel endophytic fungus from Huperzia serrata which produces huperzine A.
abstract: Huperzine A is isolated from Huperzia serrata and is used for treatment of Alzheimer's disease, due to its low toxicity and long effective period. The decrease in H. serrata sources means that natural huperzine A cannot meet the needs of clinical therapy. In this study, >200 endophytic fungal strains were isolated from H. serrata, and screened using high-performance liquid chromatography. Strain ES026 produced huperzine A. Production was identified and quantified by liquid chromatography-tandem mass spectrometry, and the yield of huperzine A was 1 μg/g dried mycelium. ES026 strain was identified as Colletotrichum gloeosporioides by morphology, polymerase chain reaction with species-specific primers and rDNA internal transcribed spacer sequence. ES026 contributes to the breeding of cultivated strains with high yield of huperzine A. Meanwhile, the strain was suitable for the study of biosynthesis of huperzine A.
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Hongshan District Shizishan Street 1, Wuhan, 430070, China, shushaohua@mail.hzau.edu.cn.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Shu
authors.forename: Xinmei
authors.initials: X
authors.lastname: Zhao
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Wang
authors.forename: Guowei
authors.initials: G
authors.lastname: Zhang
authors.forename: Andreea
authors.initials: A
authors.lastname: Cosoveanu
authors.forename: Youngjoon
authors.initials: Y
authors.lastname: Ahn
authors.forename: Mo
authors.initials: M
authors.lastname: Wang
mesh_terms: D000470:Alkaloids / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D016000:Cluster Analysis; D020231:Colletotrichum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D060026:Endophytes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D032501:Huperzia / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012717:Sesquiterpenes / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D000470:Alkaloids; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012717:Sesquiterpenes; C050426:huperzine A