title: Spatially Organized Films from Bdellovibrio bacteriovorus Prey Lysates.
abstract: Bdellovibrio bacteriovorus is a Gram-negative predator of other Gram-negative bacteria. Interestingly, Bdellovibrio bacteriovorus 109J cells grown in coculture with Escherichia coli ML-35 prey develop into a spatially organized two-dimensional film when located on a nutrient-rich surface. From deposition of 10 μl of a routine cleared coculture of B. bacteriovorus and E. coli cells, the cells multiply into a macroscopic community and segregate into an inner, yellow circular region and an outer, off-white region. Fluorescence in situ hybridization and atomic force microscopy measurements confirm that the mature film is spatially organized into two morphologically distinct Bdellovibrio populations, with primarily small, vibroid cells in the center and a complex mixture of pleomorphic cells in the outer radii. The interior region cell population exhibits the hunting phenotype while the outer region cell subpopulation does not. Crowding and high nutrient availability with limited prey appear to favor diversification of the B. bacteriovorus population into two distinct, thriving subpopulations and may be beneficial to the persistence of B. bacteriovorus in biofilms.
authors.affiliation: Department of Chemistry, State University of New York at New Paltz, New Paltz, New York, USA.
authors.forename: Megan A
authors.initials: MA
authors.lastname: Ferguson
authors.affiliation: Department of Chemistry, Wellesley College, Wellesley, Massachusetts, USA.
authors.forename: Megan E
authors.initials: ME
authors.lastname: Núñez
authors.affiliation: Department of Chemistry, Occidental College, Los Angeles, California, USA.
authors.forename: Hyeong-Jin
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Biology, Occidental College, Los Angeles, California, USA.
authors.forename: Shana
authors.initials: S
authors.lastname: Goffredi
authors.affiliation: Department of Chemistry, Occidental College, Los Angeles, California, USA.
authors.forename: Elya
authors.initials: E
authors.lastname: Shamskhou
authors.affiliation: Department of Chemistry, Occidental College, Los Angeles, California, USA.
authors.forename: Leanna
authors.initials: L
authors.lastname: Faudree
authors.affiliation: Department of Chemistry, Occidental College, Los Angeles, California, USA.
authors.forename: Evan
authors.initials: E
authors.lastname: Chang
authors.affiliation: Department of Chemistry, Occidental College, Los Angeles, California, USA.
authors.forename: Rebecca M
authors.initials: RM
authors.lastname: Landry
authors.affiliation: Department of Chemistry, Occidental College, Los Angeles, California, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Ma
authors.affiliation: Department of Chemistry, Occidental College, Los Angeles, California, USA.
authors.forename: Da-Eun
authors.initials: DE
authors.lastname: Choi
authors.affiliation: Department of Chemistry, Occidental College, Los Angeles, California, USA.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Thomas
authors.affiliation: Department of Chemistry, Occidental College, Los Angeles, California, USA.
authors.forename: Jaclyn
authors.initials: J
authors.lastname: Schmitt
authors.affiliation: Department of Chemistry, Occidental College, Los Angeles, California, USA emspain@oxy.edu.
authors.forename: Eileen M
authors.initials: EM
authors.lastname: Spain
mesh_terms: D001501:Bdellovibrio / Q000166:cytology* / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000254:growth & development*; D017404:In Situ Hybridization, Fluorescence; D056265:Microbial Interactions; D018625:Microscopy, Atomic Force
chemical_list: D003470:Culture Media