title: Alterations in the nuclear proteome of HIV-1 infected T-cells.
abstract: Virus infection of a cell involves the appropriation of host factors and the innate defensive response of the cell. The identification of proteins critical for virus replication may lead to the development of novel, cell-based inhibitors. In this study we mapped the changes in T-cell nuclei during human immunodeficiency virus type 1 (HIV-1) at 20 hpi. Using a stringent data threshold, a total of 13 and 38 unique proteins were identified in infected and uninfected cells, respectively, across all biological replicates. An additional 15 proteins were found to be differentially regulated between infected and control nuclei. STRING analysis identified four clusters of protein-protein interactions in the data set related to nuclear architecture, RNA regulation, cell division, and cell homeostasis. Immunoblot analysis confirmed the differential expression of several proteins in both C8166-45 and Jurkat E6-1 T-cells. These data provide a map of the response in host cell nuclei upon HIV-1 infection.
authors.affiliation: Department of Medical Microbiology and Immunology, Creighton University, 2500 California Plaza, Omaha, NE 68178, USA.
authors.forename: Jason
authors.initials: J
authors.lastname: DeBoer
authors.affiliation: Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198, USA.
authors.forename: Teena
authors.initials: T
authors.lastname: Jagadish
authors.affiliation: Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198, USA.
authors.forename: Nicole A
authors.initials: NA
authors.lastname: Haverland
authors.affiliation: Department of Medical Microbiology and Immunology, Creighton University, 2500 California Plaza, Omaha, NE 68178, USA.
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Madson
authors.affiliation: Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198, USA; The Nebraska Center for Virology, University of Nebraska, Lincoln 68583, USA.
authors.forename: Pawel
authors.initials: P
authors.lastname: Ciborowski
authors.affiliation: Department of Medical Microbiology and Immunology, Creighton University, 2500 California Plaza, Omaha, NE 68178, USA; The Nebraska Center for Virology, University of Nebraska, Lincoln 68583, USA. Electronic address: michaelbelshan@creighton.edu.
authors.forename: Michael
authors.initials: M
authors.lastname: Belshan
mesh_terms: D002460:Cell Line; D005786:Gene Expression Regulation / Q000276:immunology*; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism* / Q000821:virology*; D059467:Transcriptome
chemical_list: D009687:Nuclear Proteins; D020543:Proteome
keywords: HIV-1; Label-free MS/MS; Nuclear isolation; Proteomics; Virus–host interactions