title: Gender differences in normal left ventricle of adult FVB/N mice due to variation in interleukins and natriuretic peptides expression levels.
abstract: This study examined the sex differences for physical, morphological, histological, mRNA, and protein expression levels changes for interleukins and natriuretic peptides in left ventricle (LV) of two groups of adult FVB/N mice; males (WM) and females (WF). LV morphological, histological, reverse transcription and quantitative real-time PCR (RT-PCR), and immunohistochemical (IHC) alterations were determined in FVB/N mice at 34-35 weeks on gender basis. Confirming the gender dimorphism, FVB/N males (WM) illustrated a significant reduction in ANP and IL1-A levels as well as significantly increased body weight (BW (gm)), tibia length (TL (mm)), heart weight (HW (mg)), heart weight-to-body weight (HW/BW (mg/gm)) ratio, heart weight-to-tibia length (HW/TL (mg/mm)) ratio, left ventricle weight (LV (mg)), left ventricle-to-body weight (LV/BW (mg/gm)) ratio, and left ventricle-to-tibia length (LV/TL (mg/mm)) ratio, left ventricular (LV) cardiomyocyte diameter, high BNP, NPRA, IL-1B, and IL1R1 expression in comparison with FVB/N females (WF). Gender differences in relation to left ventricle (LV) may be due to differences in the interleukins and natriuretic peptides levels as an outcome of sex related hormones.
authors.affiliation: Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan; Department of Molecular Biology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan; Department of Morphology, Federal University of Minas Gerais (UFMG), 31270-010 Belo Horizonte, Minas Gerais, Brazil; Department of Physiology and Biophysics, Federal University of Minas Gerais (UFMG), 31270-010 Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Javeria
authors.initials: J
authors.lastname: Haroon
authors.affiliation: Department of Morphology, Federal University of Minas Gerais (UFMG), 31270-010 Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Giselle
authors.initials: G
authors.lastname: Foureaux
authors.affiliation: Department of Physiology and Biophysics, Federal University of Minas Gerais (UFMG), 31270-010 Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Almir S
authors.initials: AS
authors.lastname: Martins
authors.affiliation: Department of Morphology, Federal University of Minas Gerais (UFMG), 31270-010 Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Anderson J
authors.initials: AJ
authors.lastname: Ferreira
authors.affiliation: Department of Physiology and Biophysics, Federal University of Minas Gerais (UFMG), 31270-010 Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Adelina M
authors.initials: AM
authors.lastname: Reis
authors.affiliation: Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan; Department of Molecular Biology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan. Electronic address: qjaved6@gmail.com.
authors.forename: Qamar
authors.initials: Q
authors.lastname: Javed
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics / Q000378:metabolism*; D001835:Body Weight; D005260:Female; D006352:Heart Ventricles / Q000033:anatomy & histology* / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D007378:Interleukins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice; D009206:Myocardium; D032383:Myocytes, Cardiac / Q000166:cytology; D020097:Natriuretic Peptide, Brain / Q000235:genetics / Q000378:metabolism; D009929:Organ Size; D060888:Real-Time Polymerase Chain Reaction; D017461:Receptors, Atrial Natriuretic Factor / Q000235:genetics / Q000378:metabolism; D053573:Receptors, Interleukin-1 Type I / Q000235:genetics; D012727:Sex Characteristics; D013977:Tibia / Q000033:anatomy & histology
chemical_list: C583355:IL1R1 protein, mouse; D007378:Interleukins; D053573:Receptors, Interleukin-1 Type I; D020097:Natriuretic Peptide, Brain; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor; C091715:atrial natriuretic factor receptor A
keywords: FVB/N mice; Interleukins; Left ventricle; Natriuretic peptides