title: Novel DNA methylation targets in oral rinse samples predict survival of patients with oral squamous cell carcinoma.
abstract: OBJECTIVE
  The objective of this study was to identify novel survival-associated biomarkers in oral rinse samples collected from patients with oral squamous cell carcinoma (OSCC).
  
  
  METHODS
  We screened for putative survival-associated markers using publicly available methylation array data from 88 OSCC tumors. Cox models were then fit to methylation array data restricted to these putative loci in oral rinse samples of 82 OSCC patients from greater Boston. Pyrosequencing assays were designed for each locus that replicated in the oral rinse samples and applied to a validation set of oral rinse samples from another 61 OSCC patients.
  
  
  RESULTS
  We identified 7 survival-associated methylation markers in oral rinse samples from OSCC patients, and have validated one, located in the body of GABBR1, by pyrosequencing.
  
  
  CONCLUSIONS
  The 7 CpG loci identified through this study represent novel prognostic biomarkers for patients with OSCC that can be detected using a non-invasive oral rinse collection technique.
authors.affiliation: Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, OH, USA. Electronic address: langevst@uc.edu.
authors.forename: Scott M
authors.initials: SM
authors.lastname: Langevin
authors.affiliation: Department of Epidemiology, Brown University, Providence, RI, USA.
authors.forename: Rondi A
authors.initials: RA
authors.lastname: Butler
authors.affiliation: Department of Epidemiology, Brown University, Providence, RI, USA.
authors.forename: Melissa
authors.initials: M
authors.lastname: Eliot
authors.affiliation: Research Program Infection and Cancer, German Cancer Research Center (DKFZ), Heidelberg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Pawlita
authors.affiliation: Department of Psychiatry and Human Behavior, Alpert Medical School of Brown University, Providence, RI, USA; Centers for Behavioral and Preventive Medicine, The Miriam Hospital, Providence, RI, USA.
authors.forename: Jennifer Z J
authors.initials: JZ
authors.lastname: Maccani
authors.affiliation: Department of Environmental Health, Boston University School of Public Health, Boston, MA, USA.
authors.forename: Michael D
authors.initials: MD
authors.lastname: McClean
authors.affiliation: Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, OH, USA; Department of Pathology and Laboratory Medicine, Brown University, Providence, RI, USA.
authors.forename: Karl T
authors.initials: KT
authors.lastname: Kelsey
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D019175:DNA Methylation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics / Q000473:pathology*; D016019:Survival Analysis; D055815:Young Adult
keywords: Epigenomics; GABBR1; Mouthwash; Oral cancer; Prognosis