title: Yeast transformation by the LiAc/SS carrier DNA/PEG method.
abstract: Efficient transformation of yeast has been the corner stone of molecular studies in yeast for numerous years. It is essential for techniques such as the yeast two-hybrid system as well as genome modification. Here I present protocols for a number of different transformation applications. A quick and easy protocol is for situations in which large numbers of transformants are not required. The high-efficiency protocol generates large numbers of transformants from small amounts of DNA that can be scaled up for the library transformation protocol. A transformation protocol for a microtiter plate format is also included. Finally, a protocol for the production of frozen competent yeast cells is included, which allows cells to be taken from the freezer at a moment's notice and transformed with high efficiency.
authors.affiliation: Department of Biochemistry and Medical Genetics, University of Manitoba, Room 336, Basic Medical Sciences Building, Bannatyne Campus, 745 Bannatyne Avenue, Winnipeg, MB, Canada, R3E 0J9, gietz@cc.umanitoba.ca.
authors.forename: R Daniel
authors.initials: RD
authors.lastname: Gietz
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000818:Animals; D015925:Cryopreservation / Q000379:methods; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D015723:Gene Library; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics; D011092:Polyethylene Glycols / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D012474:Salmon; D014170:Transformation, Genetic
chemical_list: D000085:Acetates; D011092:Polyethylene Glycols; D004247:DNA; C488804:lithium acetate