title: Plasma components and platelet activation are essential for the antimicrobial properties of autologous platelet-rich plasma: an in vitro study.
abstract: Autologous platelet concentrates are successfully adopted in a variety of medical fields to stimulate bone and soft tissue regeneration. The rationale for their use consists in the delivery of a wide range of platelet-derived bioactive molecules that promotes wound healing. In addition, antimicrobial properties of platelet concentrates have been pointed out. In this study, the effect of the platelet concentration, of the activation step and of the presence of plasmatic components on the antimicrobial activity of pure platelet-rich plasma was investigated against gram positive bacteria isolated from oral cavity. The antibacterial activity, evaluated as the minimum inhibitory concentration, was determined through the microdilution two-fold serial method. Results seem to suggest that the antimicrobial activity of platelet-rich plasma against Enterococcus faecalis, Streptococcus agalactiae, Streptococcus oralis and Staphylococcus aureus is sustained by a co-operation between plasma components and platelet-derived factors and that the activation of coagulation is a fundamental step. The findings of this study may have practical implications in the modality of application of platelet concentrates.
authors.affiliation: Laboratory of Technical Sciences for Laboratory Medicine, Department of Biomedical Science for Health, University of Milan, Milan, Italy; Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopedic Institute, Milan, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Drago
authors.affiliation: Dental Clinic, IRCCS Galeazzi Orthopedic Institute, Milan, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Bortolin
authors.affiliation: Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopedic Institute, Milan, Italy.
authors.forename: Christian
authors.initials: C
authors.lastname: Vassena
authors.affiliation: Center of Reconstructive Surgery and Osteoarticular Infection, IRCCS Galeazzi Orthopedic Institute, Milan, Italy.
authors.forename: Carlo L
authors.initials: CL
authors.lastname: Romanò
authors.affiliation: Dental Clinic, IRCCS Galeazzi Orthopedic Institute, Milan, Italy; Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy.
authors.forename: Silvio
authors.initials: S
authors.lastname: Taschieri
authors.affiliation: Dental Clinic, IRCCS Galeazzi Orthopedic Institute, Milan, Italy; Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Del Fabbro
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D013293:Enterococcus faecalis / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D009055:Mouth / Q000382:microbiology; D015539:Platelet Activation; D053657:Platelet-Rich Plasma / Q000166:cytology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology; D013292:Streptococcus agalactiae / Q000187:drug effects / Q000502:physiology; D019533:Streptococcus oralis / Q000187:drug effects / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents