title: Phylogenetic analysis of a novel H6N6 avian influenza virus isolated from a green peafowl in China and its pathogenic potential in mice.
abstract: To explore the ecology of the H6 subtype avian influenza viruses in Hebei Province, China, a long-term surveillance was conducted in 2012 among domestic poultry and birds in a wildlife park. In this study, we report the characterization of a novel H6N6 avian influenza virus isolated from a healthy green peafowl in Qinghuangdao Wildlife Park in 2012. A phylogenetic analysis indicated that the isolated H6N6 strain has the same gene constellation as the ST3367-like strains, which are mainly distributed in southern and eastern China. A mouse experiment showed that the isolate replicated efficiently in the lungs and turbinates of infected mice without previous adaptation, resulting in locally thickened alveolar septa and interstitial pneumonia. Further studies of the H6 subtype viruses are required to clarify their evolutionary pattern in north China, which will benefit disease control and pandemic preparedness for novel viruses.
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin 150040, People's Republic of China; Harbin Veterinary Research Institute, Harbin 150001, People's Republic of China; College of Animal Science and Veterinary Medicine, Liaoning Medical University, Jinzhou 121001, People's Republic of China.
authors.forename: Zhaobin
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Harbin Veterinary Research Institute, Harbin 150001, People's Republic of China.
authors.forename: Yanpeng
authors.initials: Y
authors.lastname: Ci
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin 150040, People's Republic of China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Harbin Veterinary Research Institute, Harbin 150001, People's Republic of China.
authors.forename: Liling
authors.initials: L
authors.lastname: Liu
authors.affiliation: Harbin Veterinary Research Institute, Harbin 150001, People's Republic of China.
authors.forename: Deli
authors.initials: D
authors.lastname: Wang
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin 150040, People's Republic of China. Electronic address: 18724591358@163.com.
authors.forename: Jianzhang
authors.initials: J
authors.lastname: Ma
authors.affiliation: Harbin Veterinary Research Institute, Harbin 150001, People's Republic of China. Electronic address: liyanbing@caas.cn.
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Li
authors.affiliation: Harbin Veterinary Research Institute, Harbin 150001, People's Republic of China.
authors.forename: Hualan
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D002681:China; D005260:Female; D014644:Genetic Variation; D016679:Genome, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D009980:Influenza A virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005585:Influenza in Birds / Q000821:virology*; D051379:Mice; D009439:Neuraminidase / Q000235:genetics; D009976:Orthomyxoviridae Infections / Q000473:pathology / Q000821:virology*; D010802:Phylogeny; D011200:Poultry / Q000821:virology*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D014764:Viral Proteins; C487630:NA protein, influenza A virus; D009439:Neuraminidase
keywords: Avian influenza virus; Green peafowl; H6N6; Interstitial pneumonia; Phylogenetic analysis