title: Induction and imaging of photothrombotic stroke in conscious and freely moving rats.
abstract: In experimental stroke research, anesthesia is common and serves as a major reason for translational failure. Real-time cerebral blood flow (CBF) monitoring during stroke onset can provide important information for the prediction of brain injury; however, this is difficult to achieve in clinical practice due to various technical problems. We created a photothrombotic focal ischemic stroke model utilizing our self-developed miniature headstage in conscious and freely moving rats. In this model, a high spatiotemporal resolution imager using laser speckle contrast imaging technology was integrated to acquire real-time two-dimensional CBF information during thrombosis. The feasibility, stability, and reliability of the system were tested in terms of CBF, behavior, and T2-weighted magnetic resonance imaging (MRI) findings. After completion of occlusion, the CBF in the targeted cortex of the stroke group was reduced to 16 ± 9% of the baseline value. The mean infarct volume measured by MRI 24 h postmodeling was 77 ± 11 mm3 and correlated well with CBF (R2 = 0.74). This rodent model of focal cerebral ischemia and real-time blood flow imaging opens the possibility of performing various fundamental and translational studies on stroke without the influence of anesthetics.
authors.affiliation: Shanghai Jiao Tong University, School of Biomedical Engineering, 800 Dongchuan Road, Shanghai 200240, ChinabShanghai Jiao Tong University, Med-X Research Institute, 1954 Huashan Road, Shanghai 200030, China.
authors.forename: Hongyang
authors.initials: H
authors.lastname: Lu
authors.affiliation: Shanghai Jiao Tong University, School of Biomedical Engineering, 800 Dongchuan Road, Shanghai 200240, ChinabShanghai Jiao Tong University, Med-X Research Institute, 1954 Huashan Road, Shanghai 200030, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Shanghai Jiao Tong University, Med-X Research Institute, 1954 Huashan Road, Shanghai 200030, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Shanghai Jiao Tong University, Med-X Research Institute, 1954 Huashan Road, Shanghai 200030, China.
authors.forename: Hangdao
authors.initials: H
authors.lastname: Li
authors.affiliation: Shanghai Jiao Tong University, Med-X Research Institute, 1954 Huashan Road, Shanghai 200030, China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Lu
authors.affiliation: Shanghai Jiao Tong University, School of Biomedical Engineering, 800 Dongchuan Road, Shanghai 200240, ChinabShanghai Jiao Tong University, Med-X Research Institute, 1954 Huashan Road, Shanghai 200030, China.
authors.forename: Shanbao
authors.initials: S
authors.lastname: Tong
mesh_terms: D000818:Animals; D001794:Blood Pressure; D002560:Cerebrovascular Circulation / Q000502:physiology*; D003287:Contrast Media; D003952:Diagnostic Imaging / Q000379:methods*; D004195:Disease Models, Animal; D006339:Heart Rate; D007091:Image Processing, Computer-Assisted; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D020521:Stroke / Q000473:pathology*
chemical_list: D003287:Contrast Media