title: Antibacterial properties of root canal lubricants: a comparison with commonly used irrigants.
abstract: The aim was to assess in vitro the antibacterial activity of 10 root canal lubricants. K-Y Jelly personal lubricant, RC-Prep, File-Eze, File-Rite, EndoPrep Gel, Endosure Prep Crème 15%, Prep-Rite, Glyde, SlickGel ES and Alpha Glide were selected and compared in their antimicrobial properties to seven irrigants. Serial dilutions of each agent in tryptic soy broth were inoculated with either Enterococcus faecalis or Pseudomonas aeruginosa and incubated at 37C for 24 h. During incubation bacterial growth was measured by optical density (A(600)), and samples removed for cultivation on tryptic soy broth agar. Against both test bacteria after 1 h incubation, six lubricants recorded minimum bactericidal concentrations ranging from 1/10 to 1/80, whereas the inhibitory activity of the irrigants ranged from 1/20 to 1/640. Under these conditions, several lubricants exhibited antimicrobial activity comparable with some irrigants. Three irrigants, Consepsis (containing chlorhexidine), Endosure EDTA/C (containing cetrimide) and EndoPrep Solution (containing cetrimide), showed superior antibacterial action to lubricants against both species. The irrigants containing ethylenediamine tetraacetic acid and cetrimide were the most effective against both bacterial species at all time intervals. Antimicrobial activity of the lubricants did not correlate to pH values, which ranged from 2.9 to 10.3. Root canal lubricants have antibacterial properties that may help to disinfect canals.
authors.affiliation: Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.
authors.forename: Samantha
authors.initials: S
authors.lastname: Wong
authors.forename: Lance
authors.initials: L
authors.lastname: Mundy
authors.forename: Nicholas
authors.initials: N
authors.lastname: Chandler
authors.forename: Jenine
authors.initials: J
authors.lastname: Upritchard
authors.forename: David
authors.initials: D
authors.lastname: Purton
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Tompkins
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001431:Bacteriological Techniques; D000077463:Carbamide Peroxide; D002482:Cellulose / Q000031:analogs & derivatives / Q000494:pharmacology; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D002710:Chlorhexidine / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D013293:Enterococcus faecalis / Q000187:drug effects; D005990:Glycerol / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D054327:Lubricants / Q000008:administration & dosage / Q000494:pharmacology*; D008422:Materials Testing; D008826:Microbial Sensitivity Tests; D009930:Organic Chemicals / Q000494:pharmacology; D010545:Peroxides / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D011409:Propylene Glycols / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012388:Root Canal Irrigants / Q000008:administration & dosage / Q000494:pharmacology*; D018915:Root Canal Preparation; D012973:Sodium Hypochlorite / Q000494:pharmacology; D014508:Urea / Q000031:analogs & derivatives / Q000494:pharmacology; D014885:Waxes / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D002593:Cetrimonium Compounds; C113175:Consepsis; C454541:File-Eze; C042973:K-Y jelly; D054327:Lubricants; D009930:Organic Chemicals; D010545:Peroxides; D010710:Phosphates; D011409:Propylene Glycols; D012388:Root Canal Irrigants; D014885:Waxes; D000077463:Carbamide Peroxide; C025081:RC-Prep; D014508:Urea; D002482:Cellulose; D004492:Edetic Acid; D012973:Sodium Hypochlorite; D005990:Glycerol; D002710:Chlorhexidine; D000077286:Cetrimonium
keywords: EDTA; carbamide peroxide; cetrimide; chlorhexidine; irrigant; lubricant; root canal treatment