title: Saturated fatty acid-induced miR-195 impairs insulin signaling and glycogen metabolism in HepG2 cells.
abstract: MicroRNAs (miRNAs) play an important role in insulin signaling and insulin secretion, but the role of miRNAs in the association between obesity and hepatic insulin resistance is largely unknown. This study reports that saturated fatty acid (SFA) and high fat diet (HFD) significantly induce miR-195 expression in hepatocytes, and that the insulin receptor (INSR), not insulin receptor substrate-1 (IRS-1), is a direct target of miR-195. Furthermore, the ectopic expression of miR-195 suppresses the expression of INSR, thereby impairing the insulin signaling cascade and glycogen synthesis in HepG2 cells. These findings suggest that the dysregulation of miR-195 by SFA is a detrimental factor for hepatic insulin sensitivity.
authors.affiliation: Department of Biochemistry, Dongguk University College of Medicine, Gyeongju 780-714, Republic of Korea.
authors.forename: Won-Mo
authors.initials: WM
authors.lastname: Yang
authors.affiliation: Department of Biochemistry, Dongguk University College of Medicine, Gyeongju 780-714, Republic of Korea.
authors.forename: Hyo-Jin
authors.initials: HJ
authors.lastname: Jeong
authors.affiliation: Department of Biochemistry, Dongguk University College of Medicine, Gyeongju 780-714, Republic of Korea.
authors.forename: Seung-Yoon
authors.initials: SY
authors.lastname: Park
authors.affiliation: Department of Biochemistry, Dongguk University College of Medicine, Gyeongju 780-714, Republic of Korea; Endocrine Channelopathy, Channelopathy Research Center, Dongguk University College of Medicine, Goyang 410-773, Republic of Korea. Electronic address: wanlee@dongguk.ac.kr.
authors.forename: Wan
authors.initials: W
authors.lastname: Lee
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001665:Binding Sites; D059305:Diet, High-Fat / Q000009:adverse effects; D005227:Fatty Acids / Q000494:pharmacology*; D006003:Glycogen / Q000378:metabolism*; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D007333:Insulin Resistance; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009765:Obesity / Q000378:metabolism / Q000473:pathology; D011972:Receptor, Insulin / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D020413:3' Untranslated Regions; D005227:Fatty Acids; D007328:Insulin; C552722:MIRN195 microRNA, human; C569389:MIRN195a microRNA, mouse; D035683:MicroRNAs; D006003:Glycogen; D011972:Receptor, Insulin
keywords: Insulin receptor; Insulin receptor substrate-1; Insulin resistance; MiR-195; MicroRNA; Palmitate; Saturated fatty acid