title: Differential peptidomics highlights adipokinetic hormone as key player in regulating digestion in anautogenous flesh fly, Sarcophaga crassipalpis.
abstract: Females of anautogenous flesh flies, Sarcophaga crassipalpis, need a protein meal in order to produce their first batch of eggs. This protein meal elicits an increase in midgut proteolytic activity that is under neuropeptidergic regulation. Time series of decapitation and rescue experiments of liver fed flies evidenced the need of a peptide factor released by corpora cardiaca (CC) within 4h post protein feeding in order to assure complete protein digestion. Q-Exactive quantitative differential peptidomics analysis on CC of sugar fed flies and flies 5h post protein feeding respectively, showed a unique consistent decrease in the stored amount of adipokinetic hormone (AKH) ranging between 16% up to 63%. Injection of AKH into liver fed decapitated flies as well as sugar fed intact flies resulted in dose dependent enhanced midgut proteolytic activity up to the level of intact protein fed flies. This suggests a key role of AKH in food depended reproduction.
authors.affiliation: Research Group of Insect Physiology and Molecular Ethology, KU Leuven, Naamsestraat 59, B-3000, Belgium.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Bil
authors.affiliation: Research Group of Functional Genomics and Proteomics, KU Leuven, Naamsestraat 59, B-3000, Belgium.
authors.forename: Valérie
authors.initials: V
authors.lastname: Broeckx
authors.affiliation: Research Group of Functional Genomics and Proteomics, KU Leuven, Naamsestraat 59, B-3000, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: Landuyt
authors.affiliation: Research Group of Insect Physiology and Molecular Ethology, KU Leuven, Naamsestraat 59, B-3000, Belgium. Electronic address: Roger.Huybrechts@bio.kuleuven.be.
authors.forename: Roger
authors.initials: R
authors.lastname: Huybrechts
mesh_terms: D000818:Animals; D004063:Digestion / Q000502:physiology*; D004064:Digestive System / Q000378:metabolism; D005247:Feeding Behavior; D005260:Female; D007301:Insect Hormones / Q000378:metabolism*; D008099:Liver / Q000502:physiology; D009479:Neuropeptides / Q000737:chemistry / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D059748:Proteolysis; D040901:Proteomics / Q000379:methods*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D058493:Sarcophagidae / Q000502:physiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014020:Tissue Extracts
chemical_list: D007301:Insect Hormones; D009479:Neuropeptides; D009842:Oligopeptides; D010455:Peptides; D014020:Tissue Extracts; C044833:adipokinetic hormone; D011761:Pyrrolidonecarboxylic Acid
keywords: Adipokinetic hormone; Anautogeny; Digestion; Flesh fly; Neuropeptidomics