title: In vitro interactions of Peucedanum officinale essential oil with antibiotics.
abstract: The chemical composition and antibacterial activity of Peucedanum officinale L. (Apiaceae) essential oil were examined, as well as the association between it and antibiotics: tetracycline, streptomycin and chloramphenicol. The interactions of the essential oil with antibiotics were evaluated using the microdilution checkerboard assay. Monoterpene hydrocarbons, with α-phellandrene as the dominant constituent, were the most abundant compound class of the essential oil of P. officinale. The researched essential oil exhibited slight antibacterial activity against the tested bacterial strains in vitro. On the contrary, essential oil of P. officinale possesses a great synergistic potential with chloramphenicol and tetracycline. Their combinations reduced the minimum effective dose of the antibiotic and, consequently, minimised its adverse side effects. In addition, investigated interactions are especially successful against Gram-negative bacteria, the pharmacological treatment of which is very difficult nowadays.
authors.affiliation: a Department of Pharmacy , Faculty of Medicine, University of Niš , 18000 Niš , Serbia.
authors.forename: Dragoljub L
authors.initials: DL
authors.lastname: Miladinović
authors.forename: Budimir S
authors.initials: BS
authors.lastname: Ilić
authors.forename: Branislava D
authors.initials: BD
authors.lastname: Kocić
authors.forename: Ljiljana C
authors.initials: LC
authors.lastname: Miladinović
authors.forename: Marija S
authors.initials: MS
authors.lastname: Marković
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D019661:Apiaceae / Q000737:chemistry*; D002701:Chloramphenicol / Q000737:chemistry; D000081005:Cyclohexane Monoterpenes; D004357:Drug Synergism; D006090:Gram-Negative Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D039821:Monoterpenes / Q000737:chemistry; D009822:Oils, Volatile / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D013752:Tetracycline / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D000081005:Cyclohexane Monoterpenes; D039821:Monoterpenes; D009822:Oils, Volatile; D010938:Plant Oils; C005403:alpha phellandrene; D002701:Chloramphenicol; D013752:Tetracycline
keywords: Peucedanumofficinale; checkerboard assay; essential oil; synergistic interactions