title: In solution cation-induced secondary and tertiary structure alterations of human calprotectin.
abstract: Calprotectin (CP) is widely considered to have diverse roles including growth inhibitory and apoptosis induction in a number of tumor cell lines and antimicrobial activities. As CP has been proposed to bind metal ions with high affinity, we have studied its functional and primarily its structural behavior upon Zn(2+) and Mn(2+) chelation solely and along with Ca(2+). We employed fluorescence spectroscopy and circular dichroism to determine the resulting modifications. Based upon our findings it is clear that treating CP with ions effectively weakened its natural growth inhibitory activity. Moreover, structural analysis of Zn(2+) and Mn(2+)-treated CPs indicated remarkable alterations in the regular secondary structures in favor of irregular structures while Zn(2+) and Mn(2+) treatment of CP after incubation with Ca(2+) displayed no remarkable shifts. Tertiary structure investigation using fluorescence spectroscopy showed that CP undergoes conformational changes upon Zn(2+) and Mn(2+) treatment whereby Trp residues of protein is slightly exposed to the hydrophilic environment, compactness of CP is compromised, whereas in Ca(2+)-treated CP, the tertiary structure integrity is intact upon Zn(2+) and Mn(2+) chelation. Interestingly, CP structural modifications upon Zn(2+) and Mn(2+) treatment was significantly comparable, probably due to similar radii and charges of ions. Taken all together, we have concluded that CP maintains its normal nature in Ca(2+)-loaded state when treated with Zn(2+) and Mn(2+) ions. It can be suggested that Ca(2+) not only stabilize CP structure but also helps CP to keep its structure upon metal ions chelation which is involved in host organism defense system.
authors.affiliation: Department of Basic Science, Faculty of Veterinary Medicine, Urmia University, Nazloo, Urmia, Iran, m.imani@urmia.ac.ir.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Imani
authors.forename: Yaser
authors.initials: Y
authors.lastname: Bahrami
authors.forename: Hossein Zarei
authors.initials: HZ
authors.lastname: Jaliani
authors.forename: Sussan Kaboudanian
authors.initials: SK
authors.lastname: Ardestani
mesh_terms: D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D002412:Cations / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D006801:Humans; D039841:Leukocyte L1 Antigen Complex / Q000737:chemistry* / Q000378:metabolism*; D008345:Manganese / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D055550:Protein Stability; D015032:Zinc / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002412:Cations; D039841:Leukocyte L1 Antigen Complex; D008345:Manganese; D015032:Zinc; D002118:Calcium