title: [Glucosamine and chondroitin sulfate do not enhance anticoagulation activity of warfarin in mice in vivo].
abstract: As an adverse event, it has been reported that anticoagulation activity of warfarin was enhanced by simultaneous intakes of glucosamine and chondroitin sulfate. However, it is unclear whether these is a causative relation. Therefore, in the present study, we evaluated whether glucosamine and chondroitin sulfate enhanced the anticoagulant action of warfarin in mice in vivo, focusing on hepatic cytochrome P450 (CYPs)-mediated mechanisms. Mice were fed a diet containing various doses of glucosamine or chondroitin sulfate (0, 0.3, 1% (w/w)) for 2 weeks, and given warfarin by gavage on the last 2 days of the treatment regimen. Doses of glucosamine and chondroitin sulfate were 443 mg/kg and 464 mg/kg in the 0.3% diet groups, and 1523 mg/kg and 1546 mg/kg in the 1% diet groups. We found that 1% glucosamine significantly shortened prothrombin time and thrombotest Owen in animals given warfarin. However, the two ingredients did not induce or inhibit hepatic CYPs, including (S)-warfarin hydroxylase. These findings suggest that glucosamine and chondroitin sulfate do not affect the anticoagulation activity of warfarin through hepatic CYP mediated-mechanisms.
authors.affiliation: Information Center, National Institute of Health and Nutrition.
authors.forename: Kaori
authors.initials: K
authors.lastname: Yokotani
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nakanishi
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Chiba
authors.forename: Yoko
authors.initials: Y
authors.lastname: Sato
authors.forename: Keizo
authors.initials: K
authors.lastname: Umegaki
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D002809:Chondroitin Sulfates / Q000009:adverse effects*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005944:Glucosamine / Q000009:adverse effects*; D008099:Liver / Q000201:enzymology; D008297:Male; D008813:Mice, Inbred ICR; D011517:Prothrombin Time; D014859:Warfarin / Q000494:pharmacology*
chemical_list: D000925:Anticoagulants; D014859:Warfarin; D002809:Chondroitin Sulfates; D003577:Cytochrome P-450 Enzyme System; D005944:Glucosamine