title: Stability of the wMel Wolbachia Infection following invasion into Aedes aegypti populations.
abstract: The wMel infection of Drosophila melanogaster was successfully transferred into Aedes aegypti mosquitoes where it has the potential to suppress dengue and other arboviruses. The infection was subsequently spread into two natural populations at Yorkeys Knob and Gordonvale near Cairns, Queensland in 2011. Here we report on the stability of the infection following introduction and we characterize factors influencing the ongoing dynamics of the infection in these two populations. While the Wolbachia infection always remained high and near fixation in both locations, there was a persistent low frequency of uninfected mosquitoes. These uninfected mosquitoes showed weak spatial structure at both release sites although there was some clustering around two areas in Gordonvale. Infected females from both locations showed perfect maternal transmission consistent with patterns previously established pre-release in laboratory tests. After >2 years under field conditions, the infection continued to show complete cytoplasmic incompatibility across multiple gonotrophic cycles but persistent deleterious fitness effects, suggesting that host effects were stable over time. These results point to the stability of Wolbachia infections and their impact on hosts following local invasion, and also highlight the continued persistence of uninfected individuals at a low frequency most likely due to immigration.
authors.affiliation: Bio21 Institute, Department of Genetics, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Ary A
authors.initials: AA
authors.lastname: Hoffmann
authors.affiliation: School of Biological Sciences, Monash University, Clayton, Victoria, Australia.
authors.forename: Inaki
authors.initials: I
authors.lastname: Iturbe-Ormaetxe
authors.affiliation: Bio21 Institute, Department of Genetics, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Ashley G
authors.initials: AG
authors.lastname: Callahan
authors.affiliation: Department of Zoology, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Ben L
authors.initials: BL
authors.lastname: Phillips
authors.affiliation: School of Biological Sciences, Monash University, Clayton, Victoria, Australia.
authors.forename: Katrina
authors.initials: K
authors.lastname: Billington
authors.affiliation: Bio21 Institute, Department of Genetics, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Jason K
authors.initials: JK
authors.lastname: Axford
authors.affiliation: School of Biological Sciences, Monash University, Clayton, Victoria, Australia.
authors.forename: Brian
authors.initials: B
authors.lastname: Montgomery
authors.affiliation: School of Biological Sciences, Monash University, Clayton, Victoria, Australia.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Turley
authors.affiliation: School of Biological Sciences, Monash University, Clayton, Victoria, Australia.
authors.forename: Scott L
authors.initials: SL
authors.lastname: O'Neill
mesh_terms: D000330:Aedes / Q000382:microbiology*; D000818:Animals; D005260:Female; D054884:Host-Pathogen Interactions; D006801:Humans; D011793:Queensland; D020577:Wolbachia / Q000502:physiology*