title: The natural basil flavonoid nevadensin protects against a methyleugenol-induced marker of hepatocarcinogenicity in male F344 rat.
abstract: The alkenylbenzene methyleugenol occurs naturally in a variety of spices and herbs, including basil, and their essential oils. At high dose levels methyleugenol induces hepatocarcinogenicity in rodents following bioactivation to 1'-sulfooxymethyleugenol which forms DNA adducts. This study investigated whether the inhibitory effect of the basil flavonoid nevadensin on sulfotransferase (SULT)-mediated bioactivation of methyleugenol observed in vitro would also be reflected in a reduction of DNA adduct formation and a reduction in an early marker for liver carcinogenesis in an 8-week rat study. Co-exposure to methyleugenol and nevadensin orally resulted in a significant inhibition of liver methyleugenol DNA adduct formation and in inhibition of hepatocellular altered foci induction, representing indicators for initiation of neoplasia. These results suggest that tumor formation could be lower in rodent bioassays when methyleugenol would be dosed in a matrix containing SULT inhibitors such as nevadensin compared to experiments using the pure methyleugenol.
authors.affiliation: Division of Toxicology, Wageningen University, Tuinlaan 5, 6703 HE Wageningen, The Netherlands; Department of Pathology, New York Medical College, Valhalla, NY 10595, USA; International Organization of the Flavor Industry, IOFI, rue de Rhone, 100, 1204 Geneva, Switzerland; Flavor and Extract Manufacturers Association, 1101 17th St NW, Suite 700, Washington, DC 20036 USA. Electronic address: wasma82_botany@yahoo.com.
authors.forename: Wasma
authors.initials: W
authors.lastname: Alhusainy
authors.affiliation: Department of Pathology, New York Medical College, Valhalla, NY 10595, USA.
authors.forename: Gary M
authors.initials: GM
authors.lastname: Williams
authors.affiliation: Department of Pathology, New York Medical College, Valhalla, NY 10595, USA.
authors.forename: Alan M
authors.initials: AM
authors.lastname: Jeffrey
authors.affiliation: Department of Pathology, New York Medical College, Valhalla, NY 10595, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Iatropoulos
authors.affiliation: International Organization of the Flavor Industry, IOFI, rue de Rhone, 100, 1204 Geneva, Switzerland.
authors.forename: Sean
authors.initials: S
authors.lastname: Taylor
authors.affiliation: Flavor and Extract Manufacturers Association, 1101 17th St NW, Suite 700, Washington, DC 20036 USA.
authors.forename: Timothy B
authors.initials: TB
authors.lastname: Adams
authors.affiliation: Division of Toxicology, Wageningen University, Tuinlaan 5, 6703 HE Wageningen, The Netherlands.
authors.forename: Ivonne M C M
authors.initials: IM
authors.lastname: Rietjens
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002273:Carcinogens / Q000494:pharmacology; D018736:DNA Adducts / Q000187:drug effects*; D005054:Eugenol / Q000031:analogs & derivatives / Q000494:pharmacology; D047309:Flavones / Q000627:therapeutic use*; D008099:Liver / Q000187:drug effects; D008113:Liver Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D002273:Carcinogens; D018736:DNA Adducts; D047309:Flavones; C023982:nevadensin; C005223:methyleugenol; D005054:Eugenol
keywords: Bioactivation; DNA adducts; Hepatocellular altered foci (HAF); Methyleugenol; Nevadensin; Sulfotransferase (SULT) inhibition