title: A role for 1α,25-dihydroxyvitamin d3 in the expression of circadian genes.
abstract: The active form of vitamin D, 1α,25-(OH)2D3, has been associated with metabolism control, cell growth, differentiation, antiproliferation, apoptosis, and adaptive/innate immune responses, besides its functions in the integrity of bone and calcium homeostasis. The circadian rhythm regulates a variety of biological processes, many of them related to the functions associated with 1α,25-(OH)2D3. In the present study, we determine whether 1α,25-(OH)2D3 alters the expression of circadian genes in adipose-derived stem cells (ADSCs). The effect of 1α,25-(OH)2D3 on the expression of circadian genes BMAL1 and PER2 was measured by qPCR, over a 60-h period every 4 h, in serum shocked ADSCs, serum shocked ADSCs supplemented with 1α,25-(OH)2D3, and ADSCs under the presence of only 1α,25-(OH)2D3. The results showed that 1α,25-(OH)2D3 was able to synchronize circadian clock gene expression in ADSCs. The expression of circadian genes BMAL1 and PER2 in ADSCs that contained only 1α,25-(OH)2D3 has a profile similar to that found in the ADSCs synchronized by a serum shock. The results suggest an important role of 1α,25-(OH)2D3 in the regulation of the molecular clock.
authors.affiliation: Cátedra de Hematología y Cáncer, Tecnológico de Monterrey, Monterrey, Nuevo León, México.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Gutierrez-Monreal
authors.affiliation: Cátedra de Terapia Celular, Tecnológico de Monterrey, Monterrey, Nuevo León, México.
authors.forename: Raquel
authors.initials: R
authors.lastname: Cuevas-Diaz Duran
authors.affiliation: Cátedra de Terapia Celular, Tecnológico de Monterrey, Monterrey, Nuevo León, México.
authors.forename: Jorge E
authors.initials: JE
authors.lastname: Moreno-Cuevas
authors.affiliation: Cátedra de Hematología y Cáncer, Tecnológico de Monterrey, Monterrey, Nuevo León, México sscott04@msn.com.
authors.forename: Sean-Patrick
authors.initials: SP
authors.lastname: Scott
mesh_terms: D056930:ARNTL Transcription Factors / Q000235:genetics; D002940:Circadian Rhythm / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D056950:Period Circadian Proteins / Q000235:genetics; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000235:genetics / Q000378:metabolism
chemical_list: D056930:ARNTL Transcription Factors; C538960:BMAL1 protein, human; C421902:PER2 protein, human; D056950:Period Circadian Proteins; C118756:dihydroxy-vitamin D3; D014807:Vitamin D
keywords: BMAL1; PER2; adipose-derived stem cells; serum shock; vitamin D