title: Radiosensitivity in patients suffering from chronic kidney disease.
abstract: OBJECTIVE
  Patients suffering from chronic kidney disease (CKD) exhibit a high incidence of cancer, as well as high levels of genetic damage. We hypothesized that these patients show genomic instability detected as an increased chromosomal radiosensitivity in front of the genetic damage induced by ionizing radiation.
  
  
  METHODS
  The background levels of genetic damage and the net genetic damage after in vitro irradiation with 0.5 Gy were analyzed using the micronucleus (MN) assay in peripheral blood lymphocytes. A total number of 552 individuals (179 controls and 373 CKD patients) were included in the study.
  
  
  RESULTS
  The net radiation-induced genetic damage was significantly higher in CKD patients than in controls; but no differences between those patients submitted to hemodialysis and those in pre-dialytic stages were detected. A positive correlation was observed between basal and net micronucleus frequencies in CKD patients what would indicate an underlying genetic background modulating DNA damage levels.
  
  
  CONCLUSIONS
  Our results indicate that CKD patients present genomic instability, measured as an increased chromosomal radiosensitivity in front of ionizing radiation.
authors.affiliation: Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Edifici Cn, Universitat Autònoma de Barcelona , Bellaterra, Cerdanyola del Vallès , Spain.
authors.forename: Lara
authors.initials: L
authors.lastname: Rodríguez-Ribera
authors.forename: Zuray
authors.initials: Z
authors.lastname: Corredor
authors.forename: Silvia B
authors.initials: SB
authors.lastname: Sandoval
authors.forename: Elisabet
authors.initials: E
authors.lastname: Coll
authors.forename: Irene
authors.initials: I
authors.lastname: Silva
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Diaz
authors.forename: José
authors.initials: J
authors.lastname: Ballarin
authors.forename: Ricard
authors.initials: R
authors.lastname: Marcos
authors.forename: Susana
authors.initials: S
authors.lastname: Pastor
mesh_terms: D000368:Aged; D005260:Female; D005919:Glomerular Filtration Rate / Q000528:radiation effects; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D011836:Radiation Tolerance / Q000235:genetics*; D006435:Renal Dialysis; D051436:Renal Insufficiency, Chronic / Q000235:genetics* / Q000473:pathology* / Q000503:physiopathology / Q000628:therapy
keywords: Chronic kidney disease (CKD) patients; micronucleus assay; radiosensitivity; uremic state