title: Flavonol profiles in berries of wild Vitis accessions using liquid chromatography coupled to mass spectrometry and nuclear magnetic resonance spectrometry.
abstract: The flavonol profiles of grape berry skins were analysed in order to assess phenotypic variation between six grapevines belonging to six different species: Vitis vinifera, Vitiscandicans, Vitischampinii, Vitisamurensis, Vitiscinerea and Vitisdoaniana. High-performance liquid chromatography coupled to mass spectrometry (LC-MS) and NMR spectrometry (LC-NMR) were used to separate and identify the flavonols present in these species. The combination of LC-MS and LC-NMR data resulted in the identification of eighteen flavonols. In particular, the new flavonol diglycoside and pentoside derivatives were determined. In addition, the antioxidant capacities of flavonol grape skin extracts were evaluated by using an oxygen radical absorbance capacity method (ORAC).
authors.affiliation: INRA, ISVV, UMR 1287 EGFV, 33140 Villenave d'Ornon, France.
authors.forename: Ghislaine
authors.initials: G
authors.lastname: Hilbert
authors.affiliation: Université de Bordeaux, ISVV, EA 3675 GESVAB, 33140 Villenave d'Ornon, France.
authors.forename: Hamza
authors.initials: H
authors.lastname: Temsamani
authors.affiliation: INRA, ISVV, UMR 1287 EGFV, 33140 Villenave d'Ornon, France.
authors.forename: Louis
authors.initials: L
authors.lastname: Bordenave
authors.affiliation: Université de Bordeaux, ISVV, EA 3675 GESVAB, 33140 Villenave d'Ornon, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Pedrot
authors.affiliation: Université de Bejaia, Faculté des Sciences de la Nature et de la vie, Laboratoire de Biochimie Appliquée, Bejaia 06000, Algeria.
authors.forename: Nassima
authors.initials: N
authors.lastname: Chaher
authors.affiliation: Université de Bordeaux, ISVV, EA 3675 GESVAB, 33140 Villenave d'Ornon, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Cluzet
authors.affiliation: Université de Bordeaux, ISVV, EA 3675 GESVAB, 33140 Villenave d'Ornon, France.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Delaunay
authors.affiliation: Université de Bordeaux, ISVV, EA 3675 GESVAB, 33140 Villenave d'Ornon, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Ollat
authors.affiliation: Université de Bordeaux, ISVV, UMR 1287 EGFV, 33140 Villenave d'Ornon, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Delrot
authors.affiliation: Université de Bordeaux, ISVV, EA 3675 GESVAB, 33140 Villenave d'Ornon, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Mérillon
authors.affiliation: Université de Bordeaux, ISVV, UMR 1287 EGFV, 33140 Villenave d'Ornon, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Gomès
authors.affiliation: Université de Bordeaux, ISVV, EA 3675 GESVAB, 33140 Villenave d'Ornon, France. Electronic address: tristan.richard@u-bordeaux.fr.
authors.forename: Tristan
authors.initials: T
authors.lastname: Richard
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D044948:Flavonols / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D027843:Vitis / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D044948:Flavonols
keywords: Flavonol; Grapevine; Identification; LC–MS; LC–NMR; Polyphenol; Vitis