title: Biochemical and proteomic analysis of grape berries (Vitis labruscana) during cold storage upon postharvest salicylic acid treatment.
abstract: Salicylic acid (SA) treatment has been widely used to maintain fruit quality during postharvest storage. To elucidate the molecular mechanism related to this treatment, the effect of SA treatment on fruit quality as well as protein expression profiles of grape berries (Vitis labruscana cv. Kyoho) during the subsequent cold storage was evaluated. As expected, SA treatment inhibited postharvest loss and chilling damage by reducing fruit softening and membrane damage and slowing weight loss. A gel-based proteomic approach was designed to screen for differentially expressed proteins in SA-treated and control grape berries. A total of 69 differentially accumulated proteins were successfully identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, which can be functionally classified into eight categories. Among these proteins, antioxidant enzymes including ascorbate peroxidase, oxidoreductase, and glutathione S-transferase were induced, and the abundances of several defense-related proteins, such as heat shock protein (HSP) and temperature-induced lipocalin, were up-regulated by SA treatment. In addition, proteins involved in carbohydrate catabolism and energy production were also induced by SA treatment. Interpretation of the data for differential accumulation of proteins revealed that the effect of SA on reducing postharvest losses and chilling damage of grape berries during cold storage may be due to activated defense responses and carbohydrate metabolism and higher levels of energy status.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University , Zhenjiang 212013, China.
authors.forename: Han
authors.initials: H
authors.lastname: Cai
authors.forename: Xiaozhuan
authors.initials: X
authors.lastname: Yuan
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Pan
authors.forename: Huan
authors.initials: H
authors.lastname: Li
authors.forename: Ziming
authors.initials: Z
authors.lastname: Wu
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
mesh_terms: D003080:Cold Temperature; D061353:Food Storage; D005638:Fruit / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D020156:Salicylic Acid / Q000494:pharmacology*; D027843:Vitis / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D020156:Salicylic Acid
keywords: chilling stress; grape; postharvest; proteomics; salicylic acid