title: Changes in key constituents of clonally propagatedArtemisia annuaL. during preparation of compressed leaf tablets for possible therapeutic use.
abstract: Artemisia annuaL., long used as a tea infusion in traditional Chinese medicine, produces artemisinin. Although artemisinin is currently used as artemisinin-based combination therapy (ACT) against malaria, oral consumption of dried leaves from the plant showed efficacy and will be less costly than ACT. Many compounds in the plant have some antimalarial activity. Unknown, however, is how these plant components change as leaves are processed into tablets for oral consumption. Here we compared extracts from fresh and dried leaf biomass with compressed leaf tablets ofA. annua. Using GC-MS, nineteen endogenous compounds, including artemisinin and several of its pathway metabolites, nine flavonoids, three monoterpenes, a coumarin, and two phenolic acids, were identified and quantified from solvent extracts to determine how levels of these compounds changed during processing. Results showed that compared to dried leaves, artemisinin, arteannuin B, artemisinic acid, chlorogenic acid, scopoletin, chrysoplenetin, and quercetin increased or remained stable with powdering and compression into tablets. Dihydroartemisinic acid, monoterpenes, and chrysoplenol-D decreased with tablet formation. Five target compounds were not detectable in any of the extracts of this cultivar. In contrast to the individually measured aglycone flavonoids, using the AlCl3method, total flavonoids increased nearly fivefold during the tablet formation. To our knowledge this is the first study documenting changes that occurred in processing dried leaves ofA. annuainto tablets. These results will improve our understanding of the potential use of not only this medicinal herb, but also others to afford better quality control of intact plant material for therapeutic use.
authors.affiliation: Department Biology & Biotechnology, Worcester Polytechnic Institute, Worcester, MA 01609 USA.
authors.forename: Pamela J
authors.initials: PJ
authors.lastname: Weathers
authors.affiliation: Department Biology & Biotechnology, Worcester Polytechnic Institute, Worcester, MA 01609 USA.
authors.forename: Melissa J
authors.initials: MJ
authors.lastname: Towler
keywords: Artemisinin; flavonoids; malaria; pACT; parasites; terpenes