title: Protective effect of Cordyceps militaris against high glucose-induced oxidative stress in human umbilical vein endothelial cells.
abstract: The protective effect of Cordyceps militaris against high glucose-induced oxidative stress in human umbilical vein endothelial cells (HUVECs), as compared with Cordyceps sinensis, was examined. The cytotoxicity of HUVECs induced by 40mM glucose was ameliorated by water extracts of C. militaris (CME) and water extracts of C. sinensis (CSE). CME and CSE inhibited the increase in ROS and NO in HUVECs induced by 40mM high glucose. Moreover, CME increased the Bcl-2/Bax ratio, modulated the mitochondrial membrane potential and reduced the caspase-3 activity in high glucose-induced HUVECs. In addition, cordycepin, a component of CME and CSE, displayed protective effects against oxidative stress, which was partly responsible for the cytoprotective effects of CME and CSE against high glucose-induced oxidative stress in HUVECs. Overall, the obtained results show C. militaris helps preventing diabetic endothelial dysfunction and related complications.
authors.affiliation: Department of Food Science and Technology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, Taiwan, ROC.
authors.forename: Heuy-Ling
authors.initials: HL
authors.lastname: Chu
authors.affiliation: Department of Biotechnology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, Taiwan, ROC.
authors.forename: Jui-Chuan
authors.initials: JC
authors.lastname: Chien
authors.affiliation: Department of Food Science and Technology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, Taiwan, ROC. Electronic address: ipdduh@mail.chna.edu.tw.
authors.forename: Pin-Der
authors.initials: PD
authors.lastname: Duh
keywords: Apoptosis; Cordyceps militaris; HUVECs; High glucose; Oxidative stress