title: First finding of subgroup-E avian leukosis virus from wild ducks in China.
abstract: To analyze the status of avian leukosis virus subgroup E (ALV-E) in wild ducks in China, we collected 276 wild ducks, including 12 species, from four provinces of China. The PCR detection for ALV-E identified four samples as positive samples and the detection rate was 1.45%. The env sequences of ALV-E were cloned and sequenced. In gp85, genes of the four ALV-E strains showed a high homology (98.1-99.5%) with ev-1, ev-3, and SD0501 and more than 90% homology with other subgroup-A and subgroup-B avian leukosis viruses. However, they showed a slightly lower identity with subgroup-J (NX0101 and HPRS103), from 47.5 to 48.1%. Simultaneously, a further comparison with ALV-E representative isolates indicated that the amino acid substitutions of the four wild duck strains were distributed throughout the gp85. In total, these results suggested that the subgroup-E avian leukosis virus has been found in wild ducks in China.
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China.
authors.forename: Ruijun
authors.initials: R
authors.lastname: Hao
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Han
authors.affiliation: College of Basic Medical Science, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
authors.forename: Lanlan
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China. Electronic address: xiangwei_zeng@163.com.
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001353:Avian Leukosis / Q000453:epidemiology* / Q000821:virology*; D001354:Avian Leukosis Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D003001:Cloning, Molecular; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D004372:Ducks / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D017385:Sequence Homology; D013045:Species Specificity
chemical_list: D017931:DNA Primers
keywords: Avian leukosis virus subgroup E; Phylogenetic analysis; Wild ducks; gp85