title: Injectable dopamine-modified poly(ethylene glycol) nanocomposite hydrogel with enhanced adhesive property and bioactivity.
abstract: A synthetic mimic of mussel adhesive protein, dopamine-modified four-armed poly(ethylene glycol) (PEG-D4), was combined with a synthetic nanosilicate, Laponite (Na(0.7+)(Mg5.5Li0.3Si8)O20(OH)4)(0.7-)), to form an injectable naoncomposite tissue adhesive hydrogel. Incorporation of up to 2 wt % Laponite significantly reduced the cure time while enhancing the bulk mechanical and adhesive properties of the adhesive due to strong interfacial binding between dopamine and Laponite. The addition of Laponite did not alter the degradation rate and cytocompatibility of PEG-D4 adhesive. On the basis of subcutaneous implantation in rat, PEG-D4 nanocomposite hydrogels elicited minimal inflammatory response and exhibited an enhanced level of cellular infiltration as compared to Laponite-free samples. The addition of Laponite is potentially a simple and effective method for promoting bioactivity in a bioinert, synthetic PEG-based adhesive while simultaneously enhancing its mechanical and adhesive properties.
authors.affiliation: Department of Biomedical Engineering and ‡Department of Chemistry, Michigan Technological University , Houghton, Michigan 49931, United States.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Hao
authors.initials: H
authors.lastname: Meng
authors.forename: Shari
authors.initials: S
authors.lastname: Konst
authors.forename: Ryan
authors.initials: R
authors.lastname: Sarmiento
authors.forename: Rupak
authors.initials: R
authors.lastname: Rajachar
authors.forename: Bruce P
authors.initials: BP
authors.lastname: Lee
mesh_terms: D000818:Animals; D002452:Cell Count; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D019245:Compressive Strength / Q000187:drug effects; D004298:Dopamine / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D007267:Injections; D007452:Iodates / Q000737:chemistry; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D017207:Rats, Sprague-Dawley; D017640:Silicates / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D040521:Subcutaneous Tissue / Q000187:drug effects; D013997:Time Factors; D014014:Tissue Adhesives / Q000494:pharmacology*; D014867:Water / Q000737:chemistry
chemical_list: D007452:Iodates; D017640:Silicates; D014014:Tissue Adhesives; D014867:Water; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; C524813:laponite; C032285:sodium iodate; D004298:Dopamine
keywords: Laponite; biomimetic tissue adhesive; cell infiltration; dopamine; mussel adhesive proteins; subcutaneous implantation