title: Interleukin-6 (IL-6)-597 A/G (rs1800797) & -174 G/C (rs1800795) gene polymorphisms in type 2 diabetes.
abstract: OBJECTIVE
  Diabetes is a metabolic pro-inflammatory disorder characterized by chronic hyperglycaemia and increased levels of circulating cytokines suggesting a causal role for inflammation in its aetiology. In order to decipher the role of interleukin-6 (IL-6) in type 2 diabetes mellitus (T2DM) we analyzed two promoter polymorphisms -597 A/G (rs1800797) and -174 G/C (rs1800795) in T2DM cases from north India, and in healthy controls.
  
  
  METHODS
  DNA was isolated from venous blood samples of T2DM patients (n=213) and normal healthy controls (n=145). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed after biochemical analysis. The genotypic and allelic frequency distributions were analyzed.
  
  
  RESULTS
  The clinical/biochemical parameters of T2DM cases when compared to controls showed a significant difference. No significant association was observed with -597A/G polymorphism while, -174 G/C showed a highly significant association (P<0.001). In haplotypic analysis, combination of -597GFNx01/-174CFNx01 showed significant association (P=0.010).
  
  
  CONCLUSIONS
  Our data suggest that IL-6 gene polymorphisms play a prominent role in T2DM disease susceptibility in population from north India.
authors.forename: Madhukar
authors.initials: M
authors.lastname: Saxena
authors.forename: C G
authors.initials: CG
authors.lastname: Agrawal
authors.forename: Neena
authors.initials: N
authors.lastname: Srivastava
authors.affiliation: Department of Zoology, University of Lucknow, Lucknow, India.
authors.forename: Monisha
authors.initials: M
authors.lastname: Banerjee
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D040461:Gene Components; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007194:India; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D015850:Interleukin-6