title: Standing surface acoustic wave based cell coculture.
abstract: Precise reconstruction of heterotypic cell-cell interactions in vitro requires the coculture of different cell types in a highly controlled manner. In this article, we report a standing surface acoustic wave (SSAW)-based cell coculture platform. In our approach, different types of cells are patterned sequentially in the SSAW field to form an organized cell coculture. To validate our platform, we demonstrate a coculture of epithelial cancer cells and endothelial cells. Real-time monitoring of cell migration dynamics reveals increased cancer cell mobility when cancer cells are cocultured with endothelial cells. Our SSAW-based cell coculture platform has the advantages of contactless cell manipulation, high biocompatibility, high controllability, simplicity, and minimal interference of the cellular microenvironment. The SSAW technique demonstrated here can be a valuable analytical tool for various biological studies involving heterotypic cell-cell interactions.
authors.affiliation: Department of Engineering Science and Mechanics, The Pennsylvania State University , University Park, Pennsylvania 16802, United States.
authors.forename: Sixing
authors.initials: S
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Guo
authors.forename: Yuchao
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Ding
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Craig E
authors.initials: CE
authors.lastname: Cameron
authors.forename: Tony Jun
authors.initials: TJ
authors.lastname: Huang
mesh_terms: D002450:Cell Communication / Q000502:physiology; D002465:Cell Movement; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D004867:Equipment Design; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D046210:Microfluidic Analytical Techniques; D013016:Sound; D059008:Time-Lapse Imaging / Q000379:methods*
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; C069306:5-chloromethylfluorescein; C085925:calcein AM