title: Lacidipine attenuates TNF-α-induced cardiomyocyte apoptosis.
abstract: This study was designed to investigate whether lacidipine elicited a protective role on cardiomyocyte against apoptosis induced by TNF-α. Neonatal rat cardiomyocytes were randomly assigned into different groups. TUNEL staining was utilized to detect apoptosis, and caspase-3 and caspse-12 were determined. To explore the underlying mechanism, Z-ATAD-FMK (a selective caspase-12 inhibitor) was used to identify the key molecule involved. TNF-α increased caspase-3 expression, which was mediated by increased caspase-12 expression. In the meantime, apoptosis was significantly induced by TNF-α. Lacidipine lowered caspase-12 and caspase-3 expression, and cardiomyocyte apoptosis induced by TNF-α. The results suggest that lacidipine attenuates TNF-α -induced apoptosis via inhibition of caspase-12 and caspase-3 successively.
authors.affiliation: Department of Cardiology, Chengdu Medical College, Chengdu, Sichuan Province, PR China.
authors.forename: Qiu-Lin
authors.initials: QL
authors.lastname: Wang
authors.affiliation: Department of Physiology, National Key Discipline of Cell Biology, Fourth Military Medical University, Xi'an, Shaanxi Province, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Physiology, National Key Discipline of Cell Biology, Fourth Military Medical University, Xi'an, Shaanxi Province, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Feng
authors.affiliation: Department of Cardiology, Chengdu Medical College, Chengdu, Sichuan Province, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Department of Cardiology, Chengdu Medical College, Chengdu, Sichuan Province, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Physiology, National Key Discipline of Cell Biology, Fourth Military Medical University, Xi'an, Shaanxi Province, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Fan
authors.affiliation: Department of Physiology, National Key Discipline of Cell Biology, Fourth Military Medical University, Xi'an, Shaanxi Province, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Physiology, National Key Discipline of Cell Biology, Fourth Military Medical University, Xi'an, Shaanxi Province, PR China.
authors.forename: Shu-Miao
authors.initials: SM
authors.lastname: Zhang
authors.affiliation: Department of Physiology, National Key Discipline of Cell Biology, Fourth Military Medical University, Xi'an, Shaanxi Province, PR China.
authors.forename: Yue-Min
authors.initials: YM
authors.lastname: Wang
authors.affiliation: Department of Cardiosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, PR China.
authors.forename: Xue-Zeng
authors.initials: XZ
authors.lastname: Xu
authors.affiliation: Department of Cardiosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, PR China.
authors.forename: Shi-Qiang
authors.initials: SQ
authors.lastname: Yu
authors.affiliation: Department of Cardiosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, PR China. Electronic address: ydh@fmmu.edu.cn.
authors.forename: Ding-Hua
authors.initials: DH
authors.lastname: Yi
authors.affiliation: Department of Physiology, National Key Discipline of Cell Biology, Fourth Military Medical University, Xi'an, Shaanxi Province, PR China; Department of Cardiosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, PR China. Electronic address: jmpei8@fmmu.edu.cn.
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Pei
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D053454:Caspase 12 / Q000235:genetics / Q000378:metabolism; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D004095:Dihydropyridines / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D008297:Male; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000502:physiology; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D002121:Calcium Channel Blockers; D004095:Dihydropyridines; D014409:Tumor Necrosis Factor-alpha; C060285:lacidipine; D053454:Caspase 12; D053148:Caspase 3
keywords: Apoptosis; Caspase-12; Lacidipine; TNF-α