title: Ameliorative potential of Vernonia cinerea on chronic constriction injury of sciatic nerve induced neuropathic pain in rats.
abstract: The aim of the present study is to investigate the ameliorative potential of ethanolic extract of whole plant of Vernonia cinerea in the chronic constriction injury (CCI) of sciatic nerve induced neuropathic pain in rats. Behavioral parameters such as a hot plate, acetone drop, paw pressure, Von Frey hair and tail immersion tests were performed to assess the degree of thermal, chemical and mechanical hyperalgesia and allodynia. Biochemical changes in sciatic nerve tissue were ruled out by estimating thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) and total calcium levels. Ethanolic extract of Vernonia cinerea and pregabalin were administered for 14 consecutive days starting from the day of surgery. CCI of sciatic nerve has been shown to induce significant changes in behavioral, biochemical and histopathological assessments when compared to the sham control group. Vernonia cinerea attenuated in a dose dependent manner the above pathological changes induced by CCI of the sciatic nerve, which is similar to attenuation of the pregabalin pretreated group. The ameliorating effect of ethanolic extract of Vernonia cinerea against CCI of sciatic nerve induced neuropathic pain may be due to the presence of flavonoids and this effect is attributed to anti-oxidative, neuroprotective and calcium channel modulator actions of these compounds.
authors.affiliation: School of Chemical and Biotechnology, Sastra University, Tamilnadu, India.
authors.forename: Venkata R K
authors.initials: VR
authors.lastname: Thiagarajan
authors.affiliation: Department of Pharmaceutical Sciences & Research, Sankaralingam Bhuvaneshwari College of Pharmacy, Tamilnadu, India.
authors.forename: Palanichamy
authors.initials: P
authors.lastname: Shanmugam
authors.affiliation: Center for Nanotechnology & Advanced Biomaterials, School of Chemical & Biotechnology Sastra University, Tamilnadu, India.
authors.forename: Uma M
authors.initials: UM
authors.lastname: Krishnan
authors.affiliation: Department of Pharmaceutical Sciences & Drug Research, Punjabi University, Punjab, India.
authors.forename: Arunachalam
authors.initials: A
authors.lastname: Muthuraman
mesh_terms: D000818:Animals; D003250:Constriction; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D010147:Pain Measurement; D010936:Plant Extracts / Q000627:therapeutic use*; D017208:Rats, Wistar; D012585:Sciatica / Q000188:drug therapy* / Q000209:etiology; D013997:Time Factors; D035741:Vernonia / Q000737:chemistry*
chemical_list: D010936:Plant Extracts