title: Activity increase in EpoR and Epo expression by intranasal recombinant human erythropoietin (rhEpo) administration in ischemic hippocampi of adult rats.
abstract: Erythropoietin in the nervous system is a potential neuroprotective factor for cerebral ischemic damage due to specific-binding to the erythropoietin receptor, which is associated with survival mechanisms. However, the role of its receptor is unclear. Thus, this work assessed whether a low dose (500UI/Kg) of intranasal recombinant human erythropoietin administered 3h after ischemia induced changes in the activation of its receptor at the Tyr456-phosphorylated site in ischemic hippocampi in rats. The results showed that recombinant human erythropoietin after injury maintained cell survival and was associated with an increase in receptor phosphorylation at the Tyr456 site as an initial signaling step, which correlated with a neuroprotective effect.
authors.affiliation: Laboratorio de Neurobiología Celular, Department of Cellular and Molecular Biology, CUCBA, Universidad de Guadalajara Zapopan Jal., Mexico.
authors.forename: R
authors.initials: R
authors.lastname: Castañeda-Arellano
authors.affiliation: Laboratorio de Neurobiología Celular, Department of Cellular and Molecular Biology, CUCBA, Universidad de Guadalajara Zapopan Jal., Mexico.
authors.forename: A I
authors.initials: AI
authors.lastname: Feria-Velasco
authors.affiliation: Laboratorio de Neurobiología Celular, Department of Cellular and Molecular Biology, CUCBA, Universidad de Guadalajara Zapopan Jal., Mexico. Electronic address: mrivera939@gmail.com.
authors.forename: M C
authors.initials: MC
authors.lastname: Rivera-Cervantes
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection; D004921:Erythropoietin / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008297:Male; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000494:pharmacology; D010766:Phosphorylation; D017208:Rats, Wistar; D017467:Receptors, Erythropoietin / Q000378:metabolism*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D014443:Tyrosine / Q000378:metabolism
chemical_list: D018696:Neuroprotective Agents; D017467:Receptors, Erythropoietin; D011994:Recombinant Proteins; D004921:Erythropoietin; D014443:Tyrosine
keywords: EpoR phosphorylation; Erythropoietin; Intranasal delivery; Ischemia; Neuroprotection