title: Human serotonin receptor 5-HT(1A) preferentially segregates to the liquid disordered phase in synthetic lipid bilayers.
abstract: We demonstrate successful incorporation of the G protein coupled receptor 5-HT1A into giant unilamellar vesicles using an agarose rehydration method. With direct observation using fluorescence techniques, we report preferential segregation of 5-HT1A into the cholesterol-poor liquid disordered phase of the membrane, contradicting previous reports of lipid raft segregation. Furthermore, altering the concentration of cholesterol and sphingomyelin in ternary mixtures does not alter 5-HT1A segregation into the liquid disordered phase.
authors.affiliation: Mork Family Department of Chemical Engineering and Materials Science, University of Southern California , 925 Bloom Walk, Los Angeles, California 90089, United States.
authors.forename: M Gertrude
authors.initials: MG
authors.lastname: Gutierrez
authors.forename: Noah
authors.initials: N
authors.lastname: Malmstadt
mesh_terms: D006801:Humans; D008051:Lipid Bilayers / Q000138:chemical synthesis / Q000737:chemistry*; D044282:Receptor, Serotonin, 5-HT1A / Q000737:chemistry*; D053835:Unilamellar Liposomes / Q000737:chemistry
chemical_list: D008051:Lipid Bilayers; D053835:Unilamellar Liposomes; D044282:Receptor, Serotonin, 5-HT1A