title: High CD6 and low chemokine receptor expression on peripheral blood lymphocytes correlates with MRI gadolinium enhancement in MS.
abstract: Correlation between gadolinium-enhancing [Gd(+)] lesions on MRI and expression of CD6 molecules and a group of chemokine receptors on peripheral blood (PB) and cerebrospinal fluid (CSF) immune cells was measured in multiple sclerosis (MS) patients. Twenty remitting-relapsing MS patients with (n=10) and without (n=10) Gd(+) lesions entered the study. mRNA and surface expression of CD6 and CCR1, CCR2, CCR3 and CCR5 was measured by immunostaining and flow cytometry. Expression of mRNA and surface staining for CD6 in PB T lymphocytes was increased in Gd(+) compared to Gd(-) patients (p<0.01; p<0.05, respectively). CD6 mRNA correlated with the number and size of Gd(+) lesions (r=0.67, and r=0.65 respectively). mRNA and surface expression for CCR1, CCR2, and CCR3 in PB cells was lower in Gd(+) compared to Gd(-) MS patients (p<0.05, p<0.05). The frequency of cells co-expressing CD6 with CCR1 and CCR5 was low in PB T lymphocytes and high in CSF (p<0.05, p<0.05). These results suggest that Gd(+) correlates with increased expression of CD6 and decreased expression of chemokine receptors on PB T lymphocytes. Co-expression of CD6 with CCR1 and CCR5 predisposes cells for transmigration into CSF.
authors.affiliation: Department of Neurology, Medical University of Lodz, Lodz, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Jurewicz
authors.affiliation: Department of Neurology, Medical University of Lodz, Lodz, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Zaleski
authors.affiliation: Department of Neurology, Medical University of Lodz, Lodz, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Domowicz
authors.affiliation: Department of Neurology, Medical University of Lodz, Lodz, Poland. Electronic address: kselmaj@afazja.am.lodz.pl.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Selmaj
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D005682:Gadolinium; D015870:Gene Expression / Q000502:physiology*; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood* / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism; D054389:Receptors, CCR1 / Q000235:genetics / Q000378:metabolism*; D019713:Receptors, CCR5 / Q000235:genetics / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; C515300:CCR1 protein, human; C584933:CCR5 protein, human; C062279:CD6 antigen; D012333:RNA, Messenger; D054389:Receptors, CCR1; D019713:Receptors, CCR5; D005682:Gadolinium
keywords: CD6; Chemokine receptors; Gadolinium enhancing lesion; MRI; Multiple sclerosis (MS); Peripheral mononuclear cells (PBMC)