title: Sister kinetochores are mechanically fused during meiosis I in yeast.
abstract: Production of healthy gametes requires a reductional meiosis I division in which replicated sister chromatids comigrate, rather than separate as in mitosis or meiosis II. Fusion of sister kinetochores during meiosis I may underlie sister chromatid comigration in diverse organisms, but direct evidence for such fusion has been lacking. We used laser trapping and quantitative fluorescence microscopy to study native kinetochore particles isolated from yeast. Meiosis I kinetochores formed stronger attachments and carried more microtubule-binding elements than kinetochores isolated from cells in mitosis or meiosis II. The meiosis I-specific monopolin complex was both necessary and sufficient to drive these modifications. Thus, kinetochore fusion directs sister chromatid comigration, a conserved feature of meiosis that is fundamental to Mendelian inheritance.
authors.affiliation: Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA.
authors.forename: Krishna K
authors.initials: KK
authors.lastname: Sarangapani
authors.affiliation: Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK.
authors.forename: Eris
authors.initials: E
authors.lastname: Duro
authors.affiliation: Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK.
authors.forename: Flavia de Lima
authors.initials: Fde L
authors.lastname: Alves
authors.affiliation: Ludwig Institute for Cancer Research, San Diego Branch, La Jolla, CA 92093, USA.
authors.forename: Qiaozhen
authors.initials: Q
authors.lastname: Ye
authors.affiliation: Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA.
authors.forename: Kwaku N
authors.initials: KN
authors.lastname: Opoku
authors.affiliation: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
authors.forename: Steven
authors.initials: S
authors.lastname: Ceto
authors.affiliation: Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK. Institute of Bioanalytics, Department of Biotechnology, Technische Universität Berlin, Berlin, Germany.
authors.forename: Juri
authors.initials: J
authors.lastname: Rappsilber
authors.affiliation: Ludwig Institute for Cancer Research, San Diego Branch, La Jolla, CA 92093, USA. Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Corbett
authors.affiliation: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA. Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
authors.forename: Sue
authors.initials: S
authors.lastname: Biggins
authors.affiliation: Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK. casbury@u.washington.edu adele.marston@ed.ac.uk.
authors.forename: Adèle L
authors.initials: AL
authors.lastname: Marston
authors.affiliation: Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA. casbury@u.washington.edu adele.marston@ed.ac.uk.
authors.forename: Charles L
authors.initials: CL
authors.lastname: Asbury
mesh_terms: D047389:Casein Kinase I / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002842:Chromatids / Q000378:metabolism; D018386:Kinetochores / Q000378:metabolism*; D008540:Meiosis; D008856:Microscopy, Fluorescence; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D052898:Optical Tweezers; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C474335:CSM1 protein, S cerevisiae; D018797:Cell Cycle Proteins; C474336:LRS4 protein, S cerevisiae; C518266:Mam1 protein, S cerevisiae; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D047389:Casein Kinase I; C070775:HRR25 protein, S cerevisiae