title: Attenuation of allergic immune response phenotype by mannosylated egg white in orally induced allergy in BALB/c mice.
abstract: Food allergies are attributed to an imbalance in immune response to ubiquitous antigens. A previous study demonstrated that mannose glycation (mannosylation) of ovalbumin decreased allergenicity in vivo. The proposed research targets mannosylation of various common allergens that may help prevent food allergy. Balb/c mice (n = 8) were sensitized toxin egg white, peanut, and whey and treated with mannosylated forms of the test antigens. Glucosylated peanut and cholera toxins were used as controls. Allergic status was assessed as clinical signs, serum histamine, mouse mast cell protease (MMCP), antibody activity, cytokines, and T regulatory cells (T-regs). Significant preventative effects were observed with mannosylated egg white treatment such as reduced clinical signs, histamine, MMCP, specific G, G1, and E antibody activities, and IL-4 and increased IL-10 and CD25(+) Foxp3(+) cells. Other groups did not differ significantly. It was concluded that mannosylated egg white provides a powerful tool to prevent allergic phenotypes with possible relevance to control human egg allergy.
authors.affiliation: Department of Food Science, Ontario Agricultural College, University of Guelph , Guelph, Ontario N1G 2W1, Canada.
authors.forename: Prithy
authors.initials: P
authors.lastname: Rupa
authors.forename: Soichiro
authors.initials: S
authors.lastname: Nakamura
authors.forename: Shigeru
authors.initials: S
authors.lastname: Katayama
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Mine
mesh_terms: D000818:Animals; D010367:Arachis / Q000737:chemistry / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D021181:Egg Hypersensitivity / Q000276:immunology / Q000628:therapy; D004529:Egg White / Q000737:chemistry*; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology* / Q000628:therapy*; D006632:Histamine / Q000276:immunology; D006801:Humans; D007108:Immune Tolerance; D007073:Immunoglobulin E / Q000276:immunology; D007167:Immunotherapy; D008358:Mannose / Q000737:chemistry / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D016207:Cytokines; D007073:Immunoglobulin E; D006632:Histamine; D008358:Mannose
keywords: egg white; food allergy; immune tolerance; mannosylation; peanut; whey