title: Usefulness and limitations of polymerase chain reaction in the etiologic diagnosis of neurotoxoplasmosis in immunocompromised patients.
abstract: The objective of the present study was to assess the performance and the best indication of the polymerase chain reaction (PCR) for the detection of Toxoplasmosis gondii DNA in cerebrospinal fluid (CSF) from patients with suspected neurotoxoplasmosis. CSF samples were collected from 79 patients for amplification of the T. gondii genome (gene B1) by two PCR techniques (nested and real time). Twenty-seven of the 79 patients were classified as probable cases of neurotoxoplasmosis on the basis of clinical criteria, neuroimaging and therapeutic response. PCR showed high sensitivity (86.6%) when performed in CSF samples which were collected up to the seventh day of specific toxoplasmosis treatment. There was no positive test after 1 week of treatment. These results suggest the usefulness of PCR for the diagnosis of cerebral toxoplasmosis, and support the first week as the window for the best performance of toxoplasmosis PCR in CSF.
authors.affiliation: School of Medicine of Ribeirão Preto, University of São Paulo, Department of Neurology, Brazil. Electronic address: liviaanselmo@usp.br.
authors.forename: Lívia Maria Pala
authors.initials: LM
authors.lastname: Anselmo
authors.affiliation: Clinics Hospital, School of Medicine of Ribeirão Preto, University of São Paulo, Department of Internal Medicine - Infectious Diseases, Brazil. Electronic address: fcvilar@gmail.com.
authors.forename: Fernando Crivelenti
authors.initials: FC
authors.lastname: Vilar
authors.affiliation: Clinics Hospital, School of Medicine of Ribeirão Preto, University of São Paulo, Department of Neurology, Brazil. Electronic address: jedulima@yahoo.com.br.
authors.forename: José Eduardo
authors.initials: JE
authors.lastname: Lima
authors.affiliation: School of Medicine of Ribeirão Preto, University of São Paulo, Department of Pediatrics-Molecular and Infectious Diseases, Brazil. Electronic address: yulie@fmrp.usp.br.
authors.forename: Aparecida Yule
authors.initials: AY
authors.lastname: Yamamoto
authors.affiliation: School of Medicine of Ribeirão Preto, University of São Paulo, Department of Internal Medicine - Infectious Diseases, Brazil. Electronic address: bollela@gmail.com.
authors.forename: Valdes Roberto
authors.initials: VR
authors.lastname: Bollela
authors.affiliation: School of Medicine of Ribeirão Preto, University of São Paulo, Department of Neurology, Brazil. Electronic address: omtakay@fmrp.usp.br.
authors.forename: Osvaldo Massaiti
authors.initials: OM
authors.lastname: Takayanagui
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D016867:Immunocompromised Host; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000235:genetics*; D016781:Toxoplasmosis, Cerebral / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D055815:Young Adult
keywords: Acquired immunodeficiency syndrome; Central nervous system; Cerebrospinal fluid; HIV; Toxoplasmosis