title: Drug response of captured BT20 cells and evaluation of circulating tumor cells on a silicon nanowire platform.
abstract: Research on specific drug responses of circulating tumor cells (CTCs) provides very important information for treatment of cancer patients at a patient-specific level. For this reason, platforms for high capture efficiency of CTCs are essential for clinical evaluation of patient-specific drug responses of CTCs. Recently, nanostructure based platforms have been developed with a high capture efficiency of more than 93% due to high-affinity binding and the 3D nanotopographic features of the nanostructure substrate. In this study, the breast carcinoma cell-line (BT20) cells with an ultra-low abundance range were captured by streptavidin (STR)-functionalized silicon nanowire (SiNW) platforms for evaluation of capture efficiency. A capture efficiency of more than 90% was achieved. Specific drug responses of BT20 cells captured on STR-SiNW platforms were analyzed using tamoxifen or docetaxel as a function of incubation time and dose, and compared with a 96-well plate platform. The drug responses of CTCs on STR-SiNW platforms were more sensitive than a 96-well plate platform. In addition, CTCs were successfully captured and evaluated their size distribution from the blood of breast cancer patients using fluorescence imaging. In conclusion, we suggest that the SiNW platform is adaptable for clinical use in evaluation of CTCs and drug response tests.
authors.affiliation: Department of Physics, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Dong-Joo
authors.initials: DJ
authors.lastname: Kim
authors.affiliation: Department of Physics, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Won-Yong
authors.initials: WY
authors.lastname: Lee
authors.affiliation: Department of Physics, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: No-Won
authors.initials: NW
authors.lastname: Park
authors.affiliation: Department of Semiconductor Science and Technology, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors.forename: Gil-Sung
authors.initials: GS
authors.lastname: Kim
authors.affiliation: Cancer Research Institute, Seoul National University Hospital, Seoul 110-744, Republic of Korea.
authors.forename: Kyung-Min
authors.initials: KM
authors.lastname: Lee
authors.affiliation: Department of Surgery, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea.
authors.forename: Jongjin
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Physics, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Mun-Ki
authors.initials: MK
authors.lastname: Choi
authors.affiliation: Department of Physics, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Gee Hee
authors.initials: GH
authors.lastname: Lee
authors.affiliation: Cancer Research Institute, Seoul National University Hospital, Seoul 110-744, Republic of Korea; Department of Surgery, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea.
authors.forename: Wonshik
authors.initials: W
authors.lastname: Han
authors.affiliation: Department of Physics, Chung-Ang University, Seoul 156-756, Republic of Korea. Electronic address: sangkwonlee@cau.ac.kr.
authors.forename: Sang-Kwon
authors.initials: SK
authors.lastname: Lee
mesh_terms: D015374:Biosensing Techniques; D001943:Breast Neoplasms / Q000097:blood* / Q000188:drug therapy; D000077143:Docetaxel; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D046210:Microfluidic Analytical Techniques; D053770:Nanowires / Q000737:chemistry*; D009360:Neoplastic Cells, Circulating / Q000187:drug effects* / Q000473:pathology; D012825:Silicon / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry; D043823:Taxoids / Q000008:administration & dosage
chemical_list: D043823:Taxoids; D000077143:Docetaxel; D019809:Streptavidin; D012825:Silicon
keywords: Breast cancer patient blood; Cell capture; Circulating tumor cells (CTCs); Drug response; Silicon nanowires; Streptavidin functionalization