title: Stress-induced mutation rates show a sigmoidal and saturable increase due to the RpoS sigma factor in Escherichia coli.
abstract: Stress-induced mutagenesis was investigated in the absence of selection for growth fitness by using synthetic biology to control perceived environmental stress in Escherichia coli. We find that controlled intracellular RpoS dosage is central to a sigmoidal, saturable three- to fourfold increase in mutation rates and associated changes in DNA repair proteins.
authors.affiliation: School of Molecular Bioscience, University of Sydney, Sydney, New South Wales 2006, Australia.
authors.forename: Ram
authors.initials: R
authors.lastname: Maharjan
authors.affiliation: School of Molecular Bioscience, University of Sydney, Sydney, New South Wales 2006, Australia tom.ferenci@sydney.edu.au.
authors.forename: Thomas
authors.initials: T
authors.lastname: Ferenci
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology*; D018628:Gene Dosage; D042541:Intracellular Space / Q000378:metabolism; D059645:Mutation Rate; D012808:Sigma Factor / Q000378:metabolism*; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria
keywords: DNA repair; Escherichia coli; dinB; mutS; mutation rates; stress