title: Localization of Na(+)-K(+)-ATPase α/β, Na(+)-K(+)-2Cl-cotransporter 1 and aquaporin-5 in human eccrine sweat glands.
abstract: In order to evaluate the function of the repaired or regenerated eccrine sweat glands, we must first localize the proteins involved in sweat secretion and absorption in normal human eccrine sweat glands. In our studies, the cellular localization of Na(+)-K(+)-ATPase α/β, Na(+)-K(+)-2Cl-cotransporter 1 (NKCC1) and aquaporin-5 (AQP5) in eccrine sweat glands were detected by immunoperoxidase labeling. The results showed that Na(+)-K(+)-ATPase α was immunolocalized in the cell membrane of the basal layer and suprabasal layer cells of the epidermis, the basolateral membrane of the secretory coils, and the cell membrane of the outer cells and the basolateral membrane of the luminal cells of the ducts. The localization of Na(+)-K(+)-ATPase β in the secretory coils was the same as Na(+)-K(+)-ATPase α, but Na(+)-K(+)-ATPase β labeling was absent in the straight ducts and epidermis. NKCC1 labeling was seen only in the basolateral membrane of the secretory coils. AQP5 was strongly localized in the apical membrane and weakly localized in the cytoplasm of secretory epithelial cells. The different distribution of these proteins in eccrine sweat glands was related to their functions in sweat secretion and absorption.
authors.affiliation: Burn and Plastic Surgery Unit, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Burn and Plastic Surgery Unit, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Shaopeng
authors.initials: S
authors.lastname: Zeng
authors.affiliation: Burn and Plastic Surgery Unit, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Burn and Plastic Surgery Unit, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China; Research Center for Translational Medicine, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China. Electronic address: lihaihong1051@126.com.
authors.forename: Haihong
authors.initials: H
authors.lastname: Li
authors.affiliation: Burn and Plastic Surgery Unit, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.affiliation: Burn and Plastic Surgery Unit, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Burn and Plastic Surgery Unit, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Xuexue
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Histology and Embryology, Shantou University Medical College, 22 Xin Ling Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Changmin
authors.initials: C
authors.lastname: Lin
authors.affiliation: Burn and Plastic Surgery Unit, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Shenyou
authors.initials: S
authors.lastname: Shu
authors.affiliation: Burn and Plastic Surgery Unit, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Sitian
authors.initials: S
authors.lastname: Xie
authors.affiliation: Burn and Plastic Surgery Unit, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Yunpu
authors.initials: Y
authors.lastname: He
authors.affiliation: Burn and Plastic Surgery Unit, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Mao
authors.affiliation: Burn and Plastic Surgery Unit, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Peng
authors.affiliation: Burn and Plastic Surgery Unit, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Lungang
authors.initials: L
authors.lastname: Shi
authors.affiliation: Burn and Plastic Surgery Unit, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China; Research Center for Translational Medicine, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Lvjun
authors.initials: L
authors.lastname: Yang
authors.affiliation: Burn and Plastic Surgery Unit, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.
authors.forename: Shijie
authors.initials: S
authors.lastname: Tang
authors.affiliation: Burns Institute, The First Affiliated Hospital, Chinese PLA General Hospital, Trauma Center of Postgraduate Medical School, 51 Fu Cheng Road, Beijing 100037, PR China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Fu
mesh_terms: D000328:Adult; D051402:Aquaporin 5 / Q000378:metabolism*; D004439:Eccrine Glands / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D064506:Solute Carrier Family 12, Member 2 / Q000378:metabolism*
chemical_list: D051402:Aquaporin 5; C578877:SLC12A2 protein, human; D064506:Solute Carrier Family 12, Member 2; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Eccrine sweat gland; Immunohistochemistry; Na(+)–K(+)-ATPase α/β; Na(+)–K(+)–2Cl-cotransporter 1, Aquaporin-5