title: Ethanol increases astrocyte aquaporin-4 expression under hyper-sodium condition.
abstract: Ethanol increases brain aquaporin-4 (AQP4) expression after traumatic brain injury (TBI), leading to augment mortality and morbidity after TBI. AQP4 is regulated by sodium ion channels/transporters. Ethanol affects the ion channels/transporters. From these findings, we hypothesized that ethanol may have different effects on AQP4 expression in hypo- or hyper-sodium condition. In this study, rat primary astrocytes were incubated in iso-, hypo- or hyper-sodium MEM medium with 10% calf serum. Ethanol was added to each medium simultaneously. And to check whether hypo/hyper-sodium condition could change AQP4 expression after ethanol exposure or not, astrocytes were incubated in iso-sodium with ethanol, followed by changed to hypo/hyper-sodium with the same concentration of ethanol. Astrocyte AQP4 expression was increased in hypo-sodium exposure. Hypo-sodium with ethanol did not change AQP4 expression significantly, on the other hand, hyper-sodium with ethanol decreased AQP4 expression for short time exposure, and increased it for long time exposure. Hyper-sodium changing increased astrocyte AQP4 expression under EtOH exposure. These findings suggest that AQP4 expression is regulated by sodium ion or ion channels/transporters. And ethanol affects sodium ion channels/transporters, which is involved in AQP4 expression under ethanol.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Katada
authors.forename: Kana
authors.initials: K
authors.lastname: Sugimoto
authors.forename: Motonori
authors.initials: M
authors.lastname: Yoshida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D051401:Aquaporin 4 / Q000235:genetics / Q000378:metabolism*; D001253:Astrocytes / Q000378:metabolism*; D001930:Brain Injuries / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000009:adverse effects*; D015870:Gene Expression / Q000187:drug effects*; D006955:Hypernatremia / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000009:adverse effects / Q000032:analysis / Q000502:physiology*; D015222:Sodium Channels / Q000187:drug effects / Q000502:physiology
chemical_list: C496032:Aqp4 protein, rat; D051401:Aquaporin 4; D003470:Culture Media; D015222:Sodium Channels; D000431:Ethanol; D012964:Sodium