title: N-hydroxysuccinimidyl p-methoxybenzoate as suitable derivative reagent for isotopic dilution assay of biogenic amines in food.
abstract: We present a simple methodology for the simultaneous identification and determination of biogenic amines in food matrices, based on the use of a stable isotope-coded derivatization and liquid chromatography tandem mass spectrometry. The tagging reagent is N-hydroxysuccinimidyl ester of d(0)/d(4) -4-methoxybenzoic acid (d(0)/d(4) -4-MBA-OSu) which mainly functionalizes primary amines. The identification and structural characterization of tagged biogenic amines were exploited by matrix-assisted laser desorption/ionization-mass spectrometry (MS) and MS/MS. Multiple-reaction monitoring has been applied in the assay of biogenic amines in different foodstuffs, providing a method whose reliability is confirmed by the values of accuracy (12%) and by the calculated analytical parameters.
authors.affiliation: Dipartimento di Chimica e Tecnologie Chimiche, Università della Calabria, Via P. Bucci Cubo 12/C, I-87036, Arcavacata di Rende, Cosenza, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Mazzotti
authors.forename: Leonardo
authors.initials: L
authors.lastname: Di Donna
authors.forename: Anna
authors.initials: A
authors.lastname: Napoli
authors.forename: Donatella
authors.initials: D
authors.lastname: Aiello
authors.forename: Carlo
authors.initials: C
authors.lastname: Siciliano
authors.forename: Constantinos M
authors.initials: CM
authors.lastname: Athanassopoulos
authors.forename: Giovanni
authors.initials: G
authors.lastname: Sindona
mesh_terms: D000818:Animals; D001679:Biogenic Amines / Q000032:analysis* / Q000737:chemistry; D002611:Cheese / Q000032:analysis; D005399:Fishes; D005504:Food Analysis / Q000379:methods*; D062425:Hydroxybenzoate Ethers / Q000737:chemistry*; D057230:Limit of Detection; D008461:Meat Products / Q000032:analysis; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013388:Succinimides / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D001679:Biogenic Amines; D062425:Hydroxybenzoate Ethers; D013388:Succinimides; C001426:N-hydroxysuccinimide
keywords: LC-MS/MS-MRM; MALDI; N-hydroxysuccinimidyl ester; biogenic amines; stable isotope-coded derivatization (ICD)