title: Effects of deuterium oxide on cell growth and vesicle speed in RBL-2H3 cells.
abstract: For the first time we show the effects of deuterium oxide on cell growth and vesicle transport in rat basophilic leukemia (RBL-2H3) cells. RBL-2H3 cells cultured with 15 moles/L deuterium showed decreased cell growth which was attributed to cells not doubling their DNA content. Experimental observations also showed an increase in vesicle speed for cells cultured in deuterium oxide. This increase in vesicle speed was not observed in deuterium oxide cultures treated with a microtubule-destabilizing drug, suggesting that deuterium oxide affects microtubule-dependent vesicle transport.
authors.affiliation: BioFrontiers Center, University of Colorado at Colorado Springs, Colorado Springs, CO, USA.
authors.forename: Roshni S
authors.initials: RS
authors.lastname: Kalkur
authors.affiliation: BioFrontiers Center, University of Colorado at Colorado Springs, Colorado Springs, CO, USA.|Department of Physics and Energy Science, University of Colorado at Colorado Springs, Colorado Springs, CO, USA.
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Ballast
authors.affiliation: BioFrontiers Center, University of Colorado at Colorado Springs, Colorado Springs, CO, USA.
authors.forename: Ashley R
authors.initials: AR
authors.lastname: Triplett
authors.affiliation: BioFrontiers Center, University of Colorado at Colorado Springs, Colorado Springs, CO, USA.|Department of Physics and Energy Science, University of Colorado at Colorado Springs, Colorado Springs, CO, USA.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Spendier
keywords: Deuterium oxide; Flow cytometry; Microtubule-dependent vesicle transport; RBL-2H3 cells; Single-particle tracking; TIRF microscopy