title: Negative pressure cavitation-microwave assisted preparation of extract of Pyrola incarnata Fisch. rich in hyperin, 2'-O-galloylhyperin and chimaphilin and evaluation of its antioxidant activity.
abstract: A novel and effective extraction method, namely negative pressure cavitation-microwave assisted extraction technique (NMAE), was developed for the preparation of extracts of Pyrola incarnata Fisch., which are rich in the main constituents hyperin, 2'-O-galloylhyperin and chimaphilin. Single factor experiments and Box-Behnken design (BBD) were combined with a response surface methodology to examine factors affecting extraction. Maximum extraction yields of hyperin, 2'-O-galloylhyperin and chimaphilin (1.339±0.029, 4.831±0.117 and 0.329±0.011mg/g, respectively) were achieved under the following optimised conditions: 700W microwave power, 50°C extraction temperature, 30:1mL/g liquid-solid ratio, -0.05MPa negative pressure, 55% ethanol concentration and 12min extraction time. First-order kinetics equation demonstrated that NMAE offered significant savings in extraction time, and enhancing extraction efficiency. Furthermore, NMAE extracts yielded excellent antioxidant activity (IC50 0.121mg/mL for DPPH 2.896mmol FeSO4/g DW FRAP).
authors.affiliation: State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Yao
authors.affiliation: State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
authors.forename: Dong-Yang
authors.initials: DY
authors.lastname: Zhang
authors.affiliation: State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Luo
authors.affiliation: State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Jin
authors.affiliation: State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
authors.forename: Yuan-Gang
authors.initials: YG
authors.lastname: Zu
authors.affiliation: Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Efferth
authors.affiliation: State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China. Electronic address: yujie_fu@163.com.
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Fu
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D008872:Microwaves; D009285:Naphthoquinones / Q000032:analysis*; D010936:Plant Extracts / Q000494:pharmacology*; D011312:Pressure; D031828:Pyrola / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D000975:Antioxidants; D009285:Naphthoquinones; D010936:Plant Extracts; C087939:2''-galloylhyperin; D005707:Gallic Acid; C021304:hyperoside; D011794:Quercetin; C057774:chimaphilin
keywords: 2′-O-galloylhyperin (PubChem CID: 53399221); Antioxidant activity; Ascorbic acid (PubChem CID: 54670067); Chimaphilin (PubChem CID: 101211); DPPH (PubChem CID: 2735032); Ethanol (PubChem CID: 702); Formic acid (PubChem CID: 284); Hyperin (PubChem CID: 5281643); Mass transfer kinetics; Methanol (PubChem CID: 887); Negative pressure cavitation-microwave assisted extraction; Pyrola incarnata Fisch.