title: Okadaic acid inhibits cell multiplication and induces apoptosis in a549 cells, a human lung adenocarcinoma cell line.
abstract: This essay aims to research the effect of okadaic acid (OA) on A549 cell multiplication, and cell apoptosis induced by OA was observed by cell morphology. MTT assay, trypan blue exclusion test (TBET), Giemsa staining method and acridine orange (AO) fluorescence staining assay were applied. The results of cell survival evaluated by TBET and colorimetric assay with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) showed: The number of A549 cells was decreased in a dose-dependent manner. Cytomorphology observation of okadaic acid-treated cells showed that cells became shrinkage and turned round, some cells floated in the nutrient medium with nucleus agglutination broken, resulting in apoptotic bodies. Above-mentioned results indicated that OA exerted significantly inhibitory effect on A549 cell multiplication due to the apoptosis induced by OA.
authors.affiliation: School of Life Sciences, Qufu Normal University Qufu, Shandong 273165, China.
authors.forename: Renjun
authors.initials: R
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Qufu Normal University Qufu, Shandong 273165, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Lv
authors.affiliation: School of Life Sciences, Qufu Normal University Qufu, Shandong 273165, China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: School of Life Sciences, Qufu Normal University Qufu, Shandong 273165, China.
authors.forename: Nana
authors.initials: N
authors.lastname: Yang
keywords: A549 cell line; Okadaic acid; apoptosis; cell multiplication