title: Distinct mitral valve proteomic profiles in rheumatic heart disease and myxomatous degeneration.
abstract: Rheumatic heart disease (RHD) affects heart-valve tissue and is the most serious consequence of group A Streptococcus infection. Myxomatous degeneration (MXD) is the most frequent valvopathy in the western world. In the present work, key protein expression alterations in the heart-valve tissue of RHD and MXD patients were identified and characterized, with controls from cadaveric organ donors. Proteins were separated by two-dimensional (2D)-electrophoresis and identified by mass spectrometry. We found 17 differentially expressed protein spots, as compared to control samples. We observed an increased expression of ASAP-2 in the RHD patients' valves, while collagen-VI, haptoglobin-related protein, prolargin, and cartilage oligomeric protein showed reduced expression. Valve tissue of MXD patients, on the other hand, presented lower expression of annexin-A1 and A2, septin-2, SOD (Cu/Zn), and transgelin. Tissue samples from both valvopathies displayed higher expression of apolipoprotein-A1. Biglycan was downexpressed in both diseases. Vimentin and lumican showed higher expression in RHD and lower in MXD. These results suggest that key pathogenetic mechanisms are intrinsically distinct in RHD and MXD.
authors.affiliation: Heart Institute (INCOR), School of Medicine, University of São Paulo, São Paulo, Brazil. ; Institute of Investigation in Immunology, National Institute for Science and Technology, University of São Paulo, São Paulo, Brazil.
authors.forename: Carlo de Oliveira
authors.initials: Cde O
authors.lastname: Martins
authors.affiliation: Heart Institute (INCOR), School of Medicine, University of São Paulo, São Paulo, Brazil. ; Institute of Investigation in Immunology, National Institute for Science and Technology, University of São Paulo, São Paulo, Brazil.
authors.forename: Keity Souza
authors.initials: KS
authors.lastname: Santos
authors.affiliation: Heart Institute (INCOR), School of Medicine, University of São Paulo, São Paulo, Brazil. ; Institute of Investigation in Immunology, National Institute for Science and Technology, University of São Paulo, São Paulo, Brazil.
authors.forename: Frederico Moraes
authors.initials: FM
authors.lastname: Ferreira
authors.affiliation: Heart Institute (INCOR), School of Medicine, University of São Paulo, São Paulo, Brazil. ; Pharma Research and Early Development, F. Hoffmann-La Roche, Basel, Switzerland.
authors.forename: Priscila Camillo
authors.initials: PC
authors.lastname: Teixeira
authors.affiliation: Heart Institute (INCOR), School of Medicine, University of São Paulo, São Paulo, Brazil.
authors.forename: Pablo Maria Alberto
authors.initials: PM
authors.lastname: Pomerantzeff
authors.affiliation: Heart Institute (INCOR), School of Medicine, University of São Paulo, São Paulo, Brazil.
authors.forename: Carlos Ma
authors.initials: CM
authors.lastname: Brandão
authors.affiliation: Heart Institute (INCOR), School of Medicine, University of São Paulo, São Paulo, Brazil.
authors.forename: Roney Orismar
authors.initials: RO
authors.lastname: Sampaio
authors.affiliation: Heart Institute (INCOR), School of Medicine, University of São Paulo, São Paulo, Brazil.
authors.forename: Guilherme S
authors.initials: GS
authors.lastname: Spina
authors.affiliation: Heart Institute (INCOR), School of Medicine, University of São Paulo, São Paulo, Brazil. ; Institute of Investigation in Immunology, National Institute for Science and Technology, University of São Paulo, São Paulo, Brazil.
authors.forename: Jorge
authors.initials: J
authors.lastname: Kalil
authors.affiliation: Heart Institute (INCOR), School of Medicine, University of São Paulo, São Paulo, Brazil. ; Institute of Investigation in Immunology, National Institute for Science and Technology, University of São Paulo, São Paulo, Brazil.
authors.forename: Luiza
authors.initials: L
authors.lastname: Guilherme
authors.affiliation: Heart Institute (INCOR), School of Medicine, University of São Paulo, São Paulo, Brazil. ; Institute of Investigation in Immunology, National Institute for Science and Technology, University of São Paulo, São Paulo, Brazil.
authors.forename: Edecio
authors.initials: E
authors.lastname: Cunha-Neto
keywords: 2DE-DIGE; IPA; heart-valve proteins; myxomatous degeneration; rheumatic heart disease