title: Evaluation of antioxidant capacity of ulvan-like polymer obtained by regioselective oxidation of gellan exopolysaccharide.
abstract: Gellan is an exopolysaccharide produced in high yield by the non-pathogenic bacterium Sphingomonas elodea ATCC 31461. In this study, four carboxylated derivatives of gellan (OG-1, OG-2, OG-3 and OG-4) with different uronics acid content were prepared using the TEMPO (nitroxyl radical 2,2,6,6-tetramethylpiperidine-1-oxyl radical) mediated oxidation and their antioxidant activities were investigated including scavenging activity of hydroxyl, superoxide anion and 1,1-diphenyl-2-picryl-hydrazyl radicals. The results of chemical analysis and (13)C NMR spectrums indicated that the modification was successful. In addition, certain derivative exhibited stronger antioxidant activity compared to that of native ones. The high uronic acids derivative (OG-4) showed the most excellent antioxidant activity in three assays. These results suggested the potential of TEMPO-mediated oxidation in developing water-soluble antioxidative polysaccharides from gellan still taking advantage of its low-cost production. The original carbohydrate structure of OG-4 derivative might find use as surrogates of ulvan, a cell-wall polysaccharides extracted from green seaweeds Ulva sp.
authors.affiliation: LPMV, Université de Picardie Jules Verne, Avenue des facultés, Le Bailly, F-80025 Amiens Cedex 1, France. Electronic address: redouan_elboutachfaiti@hotmail.com.
authors.forename: Elboutachfaiti
authors.initials: E
authors.lastname: Redouan
authors.affiliation: LPMV, Université de Picardie Jules Verne, Avenue des facultés, Le Bailly, F-80025 Amiens Cedex 1, France.
authors.forename: Petit
authors.initials: P
authors.lastname: Emmanuel
authors.affiliation: LPMV, Université de Picardie Jules Verne, Avenue des facultés, Le Bailly, F-80025 Amiens Cedex 1, France.
authors.forename: Pillon
authors.initials: P
authors.lastname: Michelle
authors.affiliation: LPMV, Université de Picardie Jules Verne, Avenue des facultés, Le Bailly, F-80025 Amiens Cedex 1, France.
authors.forename: Courtois
authors.initials: C
authors.lastname: Bernard
authors.affiliation: LPMV, Université de Picardie Jules Verne, Avenue des facultés, Le Bailly, F-80025 Amiens Cedex 1, France.
authors.forename: Courtois
authors.initials: C
authors.lastname: Josiane
authors.affiliation: GREENTECH Naturally, Biopôle Clermont Limagne, 63360 Saint Beauzire, France.
authors.forename: Delattre
authors.initials: D
authors.lastname: Cédric
keywords: Antioxidant; Gellan; Regiospecific oxidation; Rhamnoglucuronan; Ulvan like