title: Search for endophytic diazotrophs in barley seeds.
abstract: Eight endophytic isolates assigned to Pseudomonas, Azospirillum, and Bacillus genera according to pheno-genotypic features were retrieved from barley seeds under selective pressure for nitrogen-fixers. Genetic relationships among related isolates were investigated through RAPD. Six isolates displayed nitrogen-fixing ability, while all could biosynthesize indolacetic acid in vitro and showed no antibiosis effects against Azospirillum brasilense Az39, a recognized PGPR.
authors.affiliation: Cátedra de Química Biológica Vegetal Departamento de Química Biológica Universidad de Buenos AiresBuenos Aires Argentina Cátedra de Química Biológica Vegetal, Departamento de Química Biológica, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Myriam S
authors.initials: MS
authors.lastname: Zawoznik
authors.affiliation: Cátedra de Microbiología Industrial y Biotecnología Departamento de Microbiología, Inmunología y Biotecnología Facultad de Farmacia y Bioquímica Universidad de Buenos Aires Buenos Aires Argentina Cátedra de Microbiología Industrial y Biotecnología, Departamento de Microbiología, Inmunología y Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Susana C
authors.initials: SC
authors.lastname: Vázquez
authors.affiliation: Cátedra de Química Biológica Vegetal Departamento de Química Biológica Universidad de Buenos AiresBuenos Aires Argentina Cátedra de Química Biológica Vegetal, Departamento de Química Biológica, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Silvana M
authors.initials: SM
authors.lastname: Díaz Herrera
authors.affiliation: Cátedra de Química Biológica Vegetal Departamento de Química Biológica Universidad de Buenos AiresBuenos Aires Argentina Cátedra de Química Biológica Vegetal, Departamento de Química Biológica, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: María D
authors.initials: MD
authors.lastname: Groppa
mesh_terms: D000898:Antibiosis; D015806:Azospirillum brasilense / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D060026:Endophytes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001467:Hordeum / Q000382:microbiology*; D007210:Indoleacetic Acids / Q000378:metabolism; D058889:Molecular Typing; D009586:Nitrogen Fixation; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D012639:Seeds / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007210:Indoleacetic Acids; D012336:RNA, Ribosomal, 16S; C030737:indoleacetic acid
keywords: Azospirillum; Bacillus; PGPR; Pseudomonas; RAPD