title: The utility of dried blood spots for proteomic studies: looking forward to looking back.
abstract: The possibility to detect biomarkers of adult disease in early life and particularly in newborns holds enormous promise for early disease detection and prevention. Early detection of disease or potential for future disease would allow for prevention or amelioration of disease before overt symptoms develop, by lifestyle modifications, appropriate medication and monitoring. It is now increasingly important to develop the technologies that allow dried blood spots (DBS) to be utilized for protein-based studies. The use of DBS in proteome wide association studies (PWAS) may in turn allow for detection of major diseases of adulthood at the earliest possible time. This review focuses on the utility of DBS in proteomics, the main challenges, as well as the latest approaches for overcoming those, facilitating the use of DBS for detection of major diseases of adulthood at the earliest possible time.
authors.affiliation: Murdoch Childrens Research Institute, Royal Children's Hospital, Victoria, Australia; Department of Paediatrics, The University of Melbourne, Victoria, Australia.
authors.forename: Vera
authors.initials: V
authors.lastname: Ignjatovic
authors.forename: James
authors.initials: J
authors.lastname: Pitt
authors.forename: Paul
authors.initials: P
authors.lastname: Monagle
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Craig
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism*; D001800:Blood Specimen Collection / Q000379:methods*; D059788:Dried Blood Spot Testing / Q000379:methods* / Q000639:trends; D006801:Humans; D007231:Infant, Newborn; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods* / Q000639:trends; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D020543:Proteome
keywords: Disease prevention; Dried blood spots