title: Microorganisms associated to tomato seedlings growing in saline culture act as osmoprotectant.
abstract: Less than 0.5% of total water in the world is available for human consumption and agriculture. The major part of the world's water is saline and salinity in soils interferes in germination of seeds and the posterior development of the plant. In order to increase the osmotolerance of tomato, seedlings were associated with Azospirillum brasilense Cd, Azospirillum brasilense Cd transformed bacteria with a plasmid harboring a trehalose biosynthesis gene-fusion or Chlorella vulgaris. Two plant culture media: Hydroponic and Murashige and Skoog were tested. In the first set of studies seedlings were associated to single free cells meanwhile in a second set single and combined free cells were studied. A positive interaction between transformed Azospirillum and Chlorella vulagris and tomato plants was observed. Seedlings showed a salt concentration tolerance, as sodium chloride, up to 200 mM. According to our results, the association of plants with A. brasilense Cd-BIF and C. vulgaris is a viable approach to increase their salt tolerance and biomass, as consequence the possible use of sea water to irrigate horticultural plants.
authors.affiliation: Department of Engineering Autonomous University of Baja California MexicaliBaja California Mexico Department of Engineering, Autonomous University of Baja California, Mexicali, Baja California, Mexico.
authors.forename: Daniel
authors.initials: D
authors.lastname: Cortés-Jiménez
authors.affiliation: Laboratory of Microbial Processes Department of Chemistry Autonomous University of Guadalajara ZapopanJalisco Mexico Laboratory of Microbial Processes, Department of Chemistry, Autonomous University of Guadalajara, Zapopan, Jalisco, Mexico. ; Department of Engineering Autonomous University of Baja California MexicaliBaja California Mexico Department of Engineering, Autonomous University of Baja California, Mexicali, Baja California, Mexico.
authors.forename: Abril
authors.initials: A
authors.lastname: Gómez-Guzmán
authors.affiliation: Centro de Investigación en Biotecnología Universidad Autónoma del Estado de Morelos CuernavacaMorelos Mexico Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Iturriaga
authors.affiliation: Centro de Investigación en Biotecnología Universidad Autónoma del Estado de Morelos CuernavacaMorelos Mexico Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
authors.forename: Ramón
authors.initials: R
authors.lastname: Suárez
authors.affiliation: Department of Engineering Autonomous University of Baja California MexicaliBaja California Mexico Department of Engineering, Autonomous University of Baja California, Mexicali, Baja California, Mexico.
authors.forename: Gisela Montero
authors.initials: GM
authors.lastname: Alpírez
authors.affiliation: Laboratory of Microbial Processes Department of Chemistry Autonomous University of Guadalajara ZapopanJalisco Mexico Laboratory of Microbial Processes, Department of Chemistry, Autonomous University of Guadalajara, Zapopan, Jalisco, Mexico.
authors.forename: Froylán M E
authors.initials: FM
authors.lastname: Escalante
mesh_terms: D015806:Azospirillum brasilense / Q000254:growth & development*; D048408:Chlorella vulgaris / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry; D018551:Solanum lycopersicum / Q000382:microbiology* / Q000502:physiology; D009997:Osmotic Pressure; D054712:Salinity; D036226:Seedlings / Q000382:microbiology* / Q000502:physiology
chemical_list: D003470:Culture Media
keywords: Trehalose; hydroponic culture; microbial association; salt tolerance