title: Expression of putative markers of pluripotency in equine embryonic and adult tissues.
abstract: Expression of several putative markers of pluripotency (OCT4, SOX2, NANOG, LIN28A, REX1, DNMT3B and TERT) was examined in a range of equine tissues, including early embryos, induced pluripotent stem cells (iPSCs), testis, adipose- and bone marrow-derived mesenchymal stromal cells (MSCs), and keratinocytes. Transcript levels of all markers were highest in embryos and iPSCs and, except for SOX2, were very low or undetectable in keratinocytes. Mean expression levels of all markers were lower in testis than in embryos or iPSCs and, except for DNMT3B, were higher in testis than in MSCs. Expression of OCT4, NANOG and DNMT3B, but not the other markers, was detected in MSCs. Of all markers analysed, only LIN28A, REX1 and TERT were associated exclusively with pluripotent cells in the horse.
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Edinburgh EH25 9RG, Scotland, UK.
authors.forename: Cristina L
authors.initials: CL
authors.lastname: Esteves
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Edinburgh EH25 9RG, Scotland, UK.
authors.forename: Ruchi
authors.initials: R
authors.lastname: Sharma
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Edinburgh EH25 9RG, Scotland, UK.
authors.forename: Lucy
authors.initials: L
authors.lastname: Dawson
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Edinburgh EH25 9RG, Scotland, UK.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Taylor
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Edinburgh EH25 9RG, Scotland, UK.
authors.forename: Gemma
authors.initials: G
authors.lastname: Pearson
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Edinburgh EH25 9RG, Scotland, UK.
authors.forename: John A
authors.initials: JA
authors.lastname: Keen
authors.affiliation: Biobest Laboratories, Edinburgh Technopole, Edinburgh EH26 0PY, UK.
authors.forename: Kieran
authors.initials: K
authors.lastname: McDonald
authors.affiliation: Artificial Insemination and Embryo Transfer, University of Veterinary Sciences, 1210 Vienna, Austria.
authors.forename: Christine
authors.initials: C
authors.lastname: Aurich
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Edinburgh EH25 9RG, Scotland, UK. Electronic address: xavier.donadeu@roslin.ed.ac.uk.
authors.forename: F Xavier
authors.initials: FX
authors.lastname: Donadeu
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D004622:Embryo, Mammalian / Q000196:embryology; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental; D006736:Horses / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism*; D015603:Keratinocytes / Q000378:metabolism*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D009928:Organ Specificity; D013737:Testis / Q000378:metabolism*
chemical_list: D015415:Biomarkers
keywords: Equine; Induced pluripotent stem cells; Mesenchymal stem/stromal cells