title: Proteome changes in mesenteric lymph induced by sepsis.
abstract: The present study aimed to examine the changes in mesenteric lymph during the development of sepsis and to identify the distinct proteins involved, as targets for further study. The sepsis animal model was constructed by cecal ligation and puncture (CLP). The mesenteric lymph was collected from 28 adult male Sprague‑Dawley rats, which were randomly divided into the following four groups (n=7 per group): CLP‑6 h, CLP‑24 h, sham‑6 h and sham‑24 h groups. Capillary high performance liquid chromatography‑tandem mass spectrometry was performed to analyze the proteome in mesenteric lymph. A comprehensive bioinformatic analysis was then conducted to investigate the distinct proteins. Compared with the sham group, 158 distinct proteins were identified in the lymph samples from the CLP group. Five of these proteins associated with the same lipid metabolism pathway were selected, apolipoprotein E (ApoE), annexin A1 (Anxa1), neutrophil gelatinase‑associated lipocalin (NGAL), S100a8 and S100a9. The expression of ApoE, Anxa1, NGAL, S100a8 and S100a9 were all elevated in the progression of sepsis. The five proteins were reported to be closely associated with disease development and may be a potential target for the diagnosis and treatment of sepsis. In conclusion, identifying proteome changes in mesenteric lymph provides a novel perspective to understand the pathological mechanisms underlying sepsis.
authors.affiliation: Emergency Department, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Emergency Department, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai 201620, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Emergency Department, Shuguang Hospital Baoshan Branch, Shanghai University of Traditional Chinese Medicine, Shanghai 201900, P.R. China.
authors.forename: Lian-Dong
authors.initials: LD
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Liang-Hua
authors.initials: LH
authors.lastname: Wang
authors.affiliation: Emergency Department, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Emergency Department, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: He
authors.affiliation: Emergency Department, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China.
authors.forename: Zhao-Fen
authors.initials: ZF
authors.lastname: Lin
mesh_terms: D000818:Animals; D002432:Cecum / Q000378:metabolism* / Q000473:pathology*; D002853:Chromatography, Liquid / Q000379:methods; D004195:Disease Models, Animal; D018450:Disease Progression; D008026:Ligation / Q000379:methods; D050356:Lipid Metabolism / Q000502:physiology; D008196:Lymph / Q000378:metabolism* / Q000502:physiology*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D011506:Proteins / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000378:metabolism* / Q000473:pathology*
chemical_list: D011506:Proteins; D020543:Proteome