title: Drosophila Ebony: a novel type of nonribosomal peptide synthetase related enzyme with unusually fast peptide bond formation kinetics.
abstract: Drosophila Ebony is a β-alanyl biogenic amine synthetase with proven function in cuticle and in glia of the nervous system. It is closely related to nonribosomal peptide synthetases (NRPSs), which typically consist of at least an adenylation, a peptidyl carrier protein and a peptide bond forming condensation domain. Besides its role in cuticle formation, Ebony is in most glia of the brain thought to convert biogenic amines to β-alanyl conjugates. If the metabolization of the neurotransmitter histamine to β-alanyl histamine requires a fast reaction in visual signal transduction, Ebony must be able to fulfill this requirement. Since NRPSs are in general slowly acting multi-modular protein machineries, the enigma of how Ebony quickly facilitates this inactivation remains a key question for understanding its role in vision. To quantitatively analyze the reaction kinetics, we used phosphopantetheinylated holo-Ebony prepared from Baculovirus infected Sf9 cells. Kinetic parameters for the loading reaction, e.g. the formation of β-alanyl-Ebony thioester, complied with those of slow NRPSs. In contrast, single-turnover analysis of the last reaction step, peptide bond formation between pre-activated β-alanyl Ebony thioester and histamine, revealed a very rapid conjugation reaction. This biphasic nature of activity identifies Ebony as a novel type of NRPS related molecule that combines a slow amino acid activation phase with a very fast product formation step.
authors.affiliation: Department of Chemistry and Biochemistry, Ruhr-University Bochum, AG Molecular Cell Biochemistry, Germany.
authors.forename: Silvia
authors.initials: S
authors.lastname: Hartwig
authors.forename: Christine
authors.initials: C
authors.lastname: Dovengerds
authors.forename: Christian
authors.initials: C
authors.lastname: Herrmann
authors.forename: Bernhard T
authors.initials: BT
authors.lastname: Hovemann
mesh_terms: D000818:Animals; D002336:Carnosine / Q000031:analogs & derivatives / Q000737:chemistry; D020134:Catalytic Domain; D004268:DNA-Binding Proteins / Q000737:chemistry*; D029721:Drosophila Proteins / Q000737:chemistry*; D004331:Drosophila melanogaster / Q000201:enzymology*; D007700:Kinetics; D010453:Peptide Synthases / Q000737:chemistry*; D011485:Protein Binding; D061987:Sf9 Cells; D018411:Spodoptera; D015091:beta-Alanine / Q000737:chemistry
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C116191:e protein, Drosophila; D015091:beta-Alanine; D002336:Carnosine; D010453:Peptide Synthases; C015055:carcinine
keywords: Drosophila; Ebony; enzyme kinetics; monoamines; nonribosomal peptide synthetases