title: Cis-12-oxo-phytodienoic acid stimulates rice defense response to a piercing-sucking insect.
abstract: The brown planthopper (BPH, Nilaparvata lugens) is a destructive, monophagous, piercing-sucking insect pest of rice. Previous studies indicated that jasmonic acid (JA) positively regulates rice defense against chewing insect pests but negatively regulates it against the piercing-sucking insect of BPH. We here demonstrated that overexpression of allene oxide cyclase (AOC) but not OPR3 (cis-12-oxo-phytodienoic acid (OPDA) reductase 3, an enzyme adjacent to AOC in the JA synthetic pathway) significantly increased rice resistance to BPH, mainly by reducing the feeding activity and survival rate. Further analysis revealed that plant response to BPH under AOC overexpression was independent of the JA pathway and that significantly higher OPDA levels stimulated rice resistance to BPH. Microarray analysis identified multiple candidate resistance-related genes under AOC overexpression. OPDA treatment stimulated the resistance of radish seedlings to green peach aphid Myzus persicae, another piercing-sucking insect. These results imply that rice resistance to chewing insects and to sucking insects can be enhanced simultaneously through AOC-mediated increases of JA and OPDA and provide direct evidence of the potential application of OPDA in stimulating plant defense responses to piercing-sucking insect pests in agriculture.
authors.affiliation: Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Guo
authors.affiliation: Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Hai-Chao
authors.initials: HC
authors.lastname: Li
authors.affiliation: National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Shi-Rong
authors.initials: SR
authors.lastname: Zhou
authors.affiliation: National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China. Electronic address: hwxue@sibs.ac.cn.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Xue
authors.affiliation: Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China. Electronic address: xxm@sibs.ac.cn.
authors.forename: Xue-Xia
authors.initials: XX
authors.lastname: Miao
mesh_terms: D000818:Animals; D003517:Cyclopentanes; D005231:Fatty Acids, Unsaturated / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D006430:Hemiptera / Q000502:physiology*; D060434:Herbivory; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000235:genetics / Q000378:metabolism; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000502:physiology
chemical_list: D003517:Cyclopentanes; D005231:Fatty Acids, Unsaturated; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C025999:12-oxophytodienoic acid; C011006:jasmonic acid; D044925:Oxidoreductases Acting on CH-CH Group Donors; C106360:12-oxophytodienoate reductase; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase
keywords: OPDA reductase 3; allene oxide cyclase; brown planthopper; cis-12-oxo-phytodienoic acid; jasmonic acid.