title: [Application of a new design of cryo-jaw and its biomechanical evaluation in rat achilles tendon in vitro].
abstract: This study was aimed to design a new, accurate and easy-to-use water bath cryo-jaw, and try to solve the problems met in small animals achilles tendon mechanical testing. The muscle-tendon-bony units were fixed in the clamps. SD rats achilles tendon were randomly divided into group A and B. Group A was tested by the newly designed water bath cryo-jaw, while group B was treated by non-water bath cryo-jaw. The mechanical tests revealed that non of the samples of the newly-designed water bath cryo-jaw in group A slipped and fell off, and the achilles tendons were in a physiologically active state, but one of the group B samples slipped and fell off, and the others had the frozen phenomenon obviously. The maximum stress, fracture displacement and Young's modulus of the rats in group A were significantly different compared to those in group B (P < 0.05). In conclusion, the new water bath cryo-jaw has more advantages than traditional ones. It exhibits a good simulation in vivo in the environmental conditions for testing the mechanical properties of the achilles tendon.
authors.forename: Erfu
authors.initials: E
authors.lastname: Liu
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Sun
authors.forename: Yongjin
authors.initials: Y
authors.lastname: Peng
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Yequan
authors.initials: Y
authors.lastname: Wang
authors.forename: Kang
authors.initials: K
authors.lastname: Xu
authors.forename: Khalid Ahmed
authors.initials: KA
authors.lastname: Mohanad
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Lu
authors.forename: Li
authors.initials: L
authors.lastname: Yang
mesh_terms: D000125:Achilles Tendon / Q000502:physiology*; D000818:Animals; D001696:Biomechanical Phenomena; D055119:Elastic Modulus; D004867:Equipment Design; D066298:In Vitro Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley