title: Surface hydroxyl groups direct cellular response on amorphous and anatase TiO2 nanodots.
abstract: In this study, we investigated the differences between amorphous and anatase TiO2 at the biomolecular level which could explain differences in the osteoblast response on these surfaces. The number of surface hydroxyl groups in the TiOHT form on amorphous and anatase TiO2 was found to be the most important factor, resulting in adsorption of bovine serum albumin as a monolayer on amorphous TiO2 nanodots but as a multilayer on anatase TiO2 nanodots. The reason for this is that the presence of more TiOHT groups on amorphous TiO2 nanodots attracts more -NH3+ groups on BSA molecules, causing the conformation of surface-bound BSA molecules to differ from those adsorbed on anatase TiO2 nanodots. Fibronectin which is subsequently adsorbed on anatase TiO2 nanodots then retains a more active conformation for osteoblast adhesion and mineralization.
authors.affiliation: Department of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hong
authors.affiliation: The Affiliated Stomatologic Hospital, Zhejiang University, Hangzhou 310003, China; The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China.
authors.forename: Mengfei
authors.initials: M
authors.lastname: Yu
authors.affiliation: The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China. Electronic address: chengkui@zju.edu.cn.
authors.forename: Kui
authors.initials: K
authors.lastname: Cheng
authors.affiliation: Department of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China; The Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, China. Electronic address: wengwj@zju.edu.cn.
authors.forename: Wenjian
authors.initials: W
authors.lastname: Weng
authors.affiliation: The Affiliated Stomatologic Hospital, Zhejiang University, Hangzhou 310003, China; The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China. Electronic address: hmwang1960@hotmail.com.
authors.forename: Huiming
authors.initials: H
authors.lastname: Wang
mesh_terms: D005353:Fibronectins / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D014025:Titanium / Q000737:chemistry*
chemical_list: D005353:Fibronectins; C009495:titanium dioxide; D014025:Titanium
keywords: Amorphous and anatase-phased TiO(2) nanodots; Cellular response; Electrostatic interactions; Surface hydroxyl groups; Surface-bound protein