title: Isolation and characterization of 8 microsatellite loci for the "killer shrimp'', an invasive Ponto-Caspian amphipod Dikerogammarus villosus (Crustacea: Amphipoda).
abstract: Dikerogammarus villosus is a freshwater amphipod of the Ponto-Caspian origin recognized as one of the 100 worst alien species in Europe, having negative impact on biodiversity and functioning of the invaded aquatic ecosystems. The species has a wide ecophysiological tolerance and during the last 20 years it has rapidly spread throughout European inland waters. In consequence, it presents a major conservation management problem. We describe eight polymorphic microsatellite loci developed for D. villosus by combining a biotin-enrichment protocol and new generation 454GS-FLX Titanium pyrosequencing technology. When genotyped in 64 individuals from two locations, the loci exhibited a mean diversity of 4.87 alleles per locus (2-13). The mean observed and expected heterozygosities were, respectively, 0.439 (0.091-0.844) and 0.468 (0.089-0.843). Gametic disequilibrium was not detected for any pair of loci. The microsatellite markers will be a valuable tool in assessing the demographic processes associated with invasion of the killer shrimp from a genetic point of view.
authors.affiliation: Department of Invertebrate Zoology and Hydrobiology, University of Lodz, 12/16 Banacha, 90-237, Lodz, Poland, tomek.rewicz@gmail.com.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Rewicz
authors.forename: Rémi A
authors.initials: RA
authors.lastname: Wattier
authors.forename: Thierry
authors.initials: T
authors.lastname: Rigaud
authors.forename: Karolina
authors.initials: K
authors.lastname: Bacela-Spychalska
authors.forename: Michal
authors.initials: M
authors.lastname: Grabowski
mesh_terms: D000483:Alleles; D033304:Amphipoda / Q000235:genetics*; D000818:Animals; D004247:DNA / Q000302:isolation & purification*; D005787:Gene Frequency / Q000235:genetics; D056426:Genetic Loci; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D004247:DNA