title: Comparison of the three optical platforms for measurement of cellular respiration.
abstract: We compared three optical platforms for measurement of cellular respiration: absolute oxygen consumption rates (OCRs) in hermetically sealed microcuvettes, relative OCRs measured in a 96-well plate with oil seal, and steady-state oxygenation of cells in an open 96-well plate. Using mouse embryonic fibroblasts cell line, the phosphorescent intracellular O2 probe MitoXpress-Intra, and time-resolved fluorescence reader, we determined algorithms for conversion of relative OCRs and cell oxygenation into absolute OCRs, thereby allowing simple high-throughput measurement of absolute OCR values.
authors.affiliation: School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland.
authors.forename: Alina V
authors.initials: AV
authors.lastname: Kondrashina
authors.affiliation: Tyndall National Institute, Dyke Parade, Cork, Ireland.
authors.forename: Vladimir I
authors.initials: VI
authors.lastname: Ogurtsov
authors.affiliation: School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland. Electronic address: d.papkovsky@ucc.ie.
authors.forename: Dmitri B
authors.initials: DB
authors.lastname: Papkovsky
mesh_terms: D000465:Algorithms; D000818:Animals; D002138:Calibration; D019069:Cell Respiration; D002478:Cells, Cultured; D005456:Fluorescent Dyes; D051379:Mice; D055096:Optical Devices; D010101:Oxygen Consumption
chemical_list: D005456:Fluorescent Dyes
keywords: Cell oxygenation; Cell respiration; Intracellular oxygen; Oxygen consumption rate