title: [Effect of acupuncture on transmembrane signal pathway in AD mice: an analysis based on lipid-raft proteomics].
abstract: OBJECTIVE
  To reveal the transmembrane signal pathway participating in regulating neuron functions of treating Alzheimer's disease (AD) by acupuncture.
  
  
  METHODS
  SAMP8 mice was used for AD animal model. The effect of acupuncture method for qi benefiting, blood regulating, health supporting, and root strengthening on the amount and varieties of transmembrane signal proteins from hippocampal lipid rafts in SAMP8 mice was detected using HPLC MS/MS proteomics method.
  
  
  RESULTS
  Compared with the control group, acupuncture increased 39 transmembrane signal proteins from hippocampal lipid rafts in SAMP8 mice, of them, 14 belonged to ionophorous protein, 8 to G protein, 8 to transmembrane signal receptor, and 9 to kinase protein. Totally 3 main cell signal pathways were involved, including G-protein-coupled receptors signal, enzyme linked receptor signal, and ion-channel mediated signal. Compared with the sham-acupuncture group, acupuncture resulted in significant increase of kinase signal protein amount. From the aspect of functions, they were dominant in regulating synapse functions relevant to cytoskeleton and secreting neurotransmitters.
  
  
  CONCLUSIONS
  The cell biological mechanism for treating AD by acupuncture might be achieved by improving synapse functions and promoting the secretion of neurotransmitters through transmembrane signal transduction, thus improving cognitive function of AD patients.
authors.forename: Kun
authors.initials: K
authors.lastname: Nie
authors.forename: Xue-Zhu
authors.initials: XZ
authors.lastname: Zhang
authors.forename: Lan
authors.initials: L
authors.lastname: Zhao
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Jia
authors.forename: Jing-Xian
authors.initials: JX
authors.lastname: Han
mesh_terms: D015670:Acupuncture Therapy; D000544:Alzheimer Disease / Q000378:metabolism*; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D021962:Membrane Microdomains / Q000378:metabolism; D051379:Mice; D040901:Proteomics; D015398:Signal Transduction; D053719:Tandem Mass Spectrometry