title: Characterization of a compartment syndrome-like injury model.
abstract: BACKGROUND
  Acute compartment syndrome (CS) is caused by an elevation of pressure within a muscular compartment that can be caused by numerous factors, including blunt trauma. In this study, we characterized a rodent model of CS-like injury.
  
  
  METHODS
  Forty male athymic rats received a standardized injury of ischemia and compression to their hindlimbs, while the intracompartmental pressure (ICP) was measured using an implantable transmitter. Tetanic muscle function was evaluated, and histology was performed on the tibialis anterior (TA) muscle.
  
  
  RESULTS
  ICPs were held at 260.70 ± 2.70 mm Hg during injury. Injured muscles recovered 59% of their total function 4 weeks after injury, and histology showed high levels of edema, inflammation (CD68(+) ), angiogenesis (CD31(+) ), and fibrosis within 72 hours after injury.
  
  
  CONCLUSIONS
  We describe a novel CS-like injury model and a novel method to measure ICP, which could potentially be used to develop innovative therapies to manage CS injury in patients.
authors.affiliation: Stem Cell Research Center, University of Pittsburgh, Suite 206, Bridgeside Point II, 450 Technology Drive, Pittsburgh, Pennsylvania, 15219, USA.
authors.forename: Nick
authors.initials: N
authors.lastname: Oyster
authors.forename: Michelle
authors.initials: M
authors.lastname: Witt
authors.forename: Burhan
authors.initials: B
authors.lastname: Gharaibeh
authors.forename: Minakshi
authors.initials: M
authors.lastname: Poddar
authors.forename: Johannes
authors.initials: J
authors.lastname: Schneppendahl
authors.forename: Johnny
authors.initials: J
authors.lastname: Huard
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D003161:Compartment Syndromes / Q000503:physiopathology*; D004195:Disease Models, Animal; D005355:Fibrosis; D008297:Male; D018482:Muscle, Skeletal / Q000293:injuries* / Q000378:metabolism / Q000503:physiopathology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D011312:Pressure / Q000009:adverse effects; D051381:Rats; D011923:Rats, Nude; D020127:Recovery of Function / Q000502:physiology; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015415:Biomarkers; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C067980:CD68 antigen, human; D000097382:CD68 Molecule
keywords: angiogenesis; compartment syndrome; fibrosis; inflammation; regeneration