title: Oncogenic nature of a novel mutant AATF and its interactome existing within human cancer cells.
abstract: Since apoptosis presents a natural defense in cancer development, the anti-apoptotic factor AATF/Che-1 has emerged as a crucial 'Epigenomic-Switch'. We have tried to understand the double-edged nature of AATF, showing for the first time the conspicuous existence of an aberrant AATF/Che-1 transcriptome encoding for 23 kDa mutant AATF protein, which evolves its unique interactome within human cancer cells derived from different tissue origins. This mutant AATF along with its interactome consisting of SP1, DNMT3B and Par-4 ensures cancer cell DNA methylation required for down-regulation of tumor suppressor genes. Hence, the proposed mutant AATF interactome-based pathway can have the inherent ability to ensure human cells become and remain cancerous.
authors.affiliation: Department of Experimental Medicine & Biotechnology, Molecular Biology Unit, Post-graduate Institute of Medical Education & Research, Chandigarh, 160012, India.
authors.forename: Shaveta
authors.initials: S
authors.lastname: Sharma
authors.forename: Deepak
authors.initials: D
authors.lastname: Kaul
authors.forename: Mansi
authors.initials: M
authors.lastname: Arora
authors.forename: Deepti
authors.initials: D
authors.lastname: Malik
mesh_terms: D000595:Amino Acid Sequence; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism*; D001343:Autophagy; D045744:Cell Line, Tumor; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D015536:Down-Regulation; D059585:G1 Phase Cell Cycle Checkpoints; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D008969:Molecular Sequence Data; D009154:Mutation; D009369:Neoplasms / Q000235:genetics* / Q000503:physiopathology; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000378:metabolism; D000097584:DNA Methyltransferase 3B
chemical_list: D051017:Apoptosis Regulatory Proteins; D004248:DNA (Cytosine-5-)-Methyltransferases; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D016329:Sp1 Transcription Factor; D000097584:DNA Methyltransferase 3B; C402637:AATF protein, human; C101296:prostate apoptosis response-4 protein
keywords: Cancer cells; DNMT3B; Mutant-AATF; Oncogenomics