title: Gold nanoparticle modified conducting polymer of 4-(2,5-di(thiophen-2-yl)-1H-pyrrole-1-l) benzenamine for potential use as a biosensing material.
abstract: Gold nanoparticle (AuNP) modified conducting polymer of 4-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)benzenamine (SNS-NH2) was used as the biosensing platform for glucose analysis. Electrochemical measurements were carried out by following the consumed oxygen due to the enzymatic reaction of glucose oxidase (GOx) at -0.7V vs Ag/AgCl. Optimisation of pH, enzyme loading, stability experiments were carried out. Effect of NP was investigated by monitoring the signal responses at different AuNP sizes and amounts. A linear relation of y=1.597x+0.264 (R(2)=0.993) was found for glucose concentrations between 0.002 and 5.0mM. The analytical characteristics of the system were also evaluated for glucose determination in flow injection analysis (FIA) mode. Finally, the system was checked for glucose detection on real samples.
authors.affiliation: Department of Chemistry and Division of Biotechnology, Middle East Technical University, 06531 Ankara, Turkiye; Department of Biotechnology, Middle East Technical University, 06531 Ankara, Turkiye.
authors.forename: Sevinc
authors.initials: S
authors.lastname: Tuncagil
authors.affiliation: Department of Biochemistry, Faculty of Science, Ege University, 35100 Bornova-Izmir, Turkiye.
authors.forename: Caglar
authors.initials: C
authors.lastname: Ozdemir
authors.affiliation: Department of Biochemistry, Faculty of Science, Ege University, 35100 Bornova-Izmir, Turkiye.
authors.forename: Dilek Odaci
authors.initials: DO
authors.lastname: Demirkol
authors.affiliation: Department of Biochemistry, Faculty of Science, Ege University, 35100 Bornova-Izmir, Turkiye. Electronic address: sunatimur@yahoo.com.
authors.forename: Suna
authors.initials: S
authors.lastname: Timur
authors.affiliation: Department of Chemistry and Division of Biotechnology, Middle East Technical University, 06531 Ankara, Turkiye; Department of Biotechnology, Middle East Technical University, 06531 Ankara, Turkiye.
authors.forename: Levent
authors.initials: L
authors.lastname: Toppare
keywords: Conducting polymers; Electrochemical biosensors; Electrochemical polymerisation; Gold nanoparticles (AuNPs)