title: [Synergistic effect of platelet-derived growth factor-BB and transforming growth factor-beta1, on expression of integrin beta3 in periodontal membrane of rat orthodontic tooth].
abstract: OBJECTIVE
  To investigate the synergistic effect of transforming growth factor-beta1 (TGF-beta1) and platelet-derived growth factor-BB (PDGF-BB) on the expression of integrin beta3, in periodontal membrane of rat orthodontic tooth.
  
  
  METHODS
  An orthodontic tooth movement model was established. Up to 32 experimental rats were randomly divided into four groups according to a random number table. The four groups were injected with 1% PBS, TGF-beta1 (5 ng), PDGF-BB (10 ng), and combined TGF-beta1 (5 ng) and PDGF-BB (10 ng) in the buccal submucosal, respectively. The volume injected in each group was 0.1 mL. The animals were then sacrificed on the 10th day. The left maxillary first molar and periodontal tissue were taken. Different expressions of integrin beta3 were detected in periodontal tissues through immunohistochemistry. Mean optical density (OD) values of the positive fields were examined. The data obtained were analyzed through ANOVA. The data followed normal distribution, and were compared via t-test.
  
  
  RESULTS
  Compared with the control groups, the expression of integrin beta3 was higher in the experimental groupin tension sides (P < 0.01). Significant differences in tension sides between the single-injection groups and the combined group were observed (P < 0.01). Compared with the control groups, the expression of integrin beta3 was higher in the experimental group in compression sides (P < 0.05). In addition, there was no significant differences in compression sides between the single-injection groups and the combined group (P > 0.05).
  
  
  CONCLUSIONS
  In terms of local regulatory factors, TGF-beta1 combined with PDGF-BB enhance the expression of integrin beta3 in the periodontal membrane and accelerate periodontal remodeling. The synergistic effect of the two growth factors is better than the single growth factor.
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.forename: Jianguo
authors.initials: J
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Song
authors.forename: Mu
authors.initials: M
authors.lastname: Su
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Guan
authors.forename: Juxiang
authors.initials: J
authors.lastname: Peng
mesh_terms: D000818:Animals; D000077214:Becaplermin; D039661:Integrin beta3; D008963:Molar; D010513:Periodontal Ligament; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D014087:Tooth Movement Techniques; D053773:Transforming Growth Factor beta1
chemical_list: D039661:Integrin beta3; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D053773:Transforming Growth Factor beta1; D000077214:Becaplermin