title: Toll-like receptor 3 transmembrane domain is able to perform various homotypic interactions: an NMR structural study.
abstract: Toll-like receptors (TLRs) take part in both the innate and adaptive immune systems. The role of the transmembrane domain in TLR signaling is still elusive, while its importance for the TLR activation was clearly demonstrated. In the present study the ability of the TLR3 transmembrane domain to form dimers and trimers in detergent micelles was shown by solution NMR spectroscopy. Spatial structures and free energy magnitudes were determined for the TLR3 transmembrane domain in dimeric and trimeric states, and two possible surfaces that may be used for the helix-helix interaction by the full-length TLR3 were revealed.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences RAS, Str. Miklukho-Maklaya 16/10, Moscow 117997, Russian Federation. Electronic address: mineev@nmr.ru.
authors.forename: Konstantin S
authors.initials: KS
authors.lastname: Mineev
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences RAS, Str. Miklukho-Maklaya 16/10, Moscow 117997, Russian Federation; Lomonosov Moscow State University, Moscow 119991, Russian Federation.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Goncharuk
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences RAS, Str. Miklukho-Maklaya 16/10, Moscow 117997, Russian Federation.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Arseniev
mesh_terms: D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000737:chemistry; D011485:Protein Binding; D055503:Protein Multimerization; D055550:Protein Stability; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D013816:Thermodynamics; D051196:Toll-Like Receptor 3 / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D008823:Micelles; C495344:TLR3 protein, human; D051196:Toll-Like Receptor 3; D010767:Phosphorylcholine; C028810:dodecylphosphocholine
keywords: Dimerization; Free energy; Spatial structure; Toll-like receptor; Transmembrane domain