title: Turn-on fluorescence sensor for the detection of heparin based on rhodamine B-modified polyethyleneimine-graphene oxide complex.
abstract: Rhodamine B modified Polyethyleneimine (PEI) (called RB-PEI) with strong fluorescence intensity was synthesized, which contained high positively charged amine groups. The fluorescence intensity of RB-PEI can be quenched by graphene oxide (GO). The high efficiency of the quenching was caused by the non-covalent binding of RB-PEI to the GO surface by electrostatic and π-π interactions, forming a RB-PEI/GO complex. However, adding heparin caused intense fluorescence to be recovered, and this was caused by the anionic heparin removing the RB-PEI from the GO surface, forming a fluorescent RB-PEI/heparin complex. Under optimal conditions, the change in the fluorescence intensity when heparin was added was proportional to the heparin concentration over the range 0.09-0.9 U mL(-1). The detection limit for heparin was 0.00132 U mL(-1), which was lower than that has been achieved using other methods. This approach offers a new quantitative method for determining heparin.
authors.affiliation: College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo/Biosensing, The Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Laboratory of Molecule-based Materials, Anhui Normal University, Wuhu 241000, PR China. Electronic address: jsliu@sina.com.
authors.forename: Jinshui
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo/Biosensing, The Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Laboratory of Molecule-based Materials, Anhui Normal University, Wuhu 241000, PR China.
authors.forename: Guoning
authors.initials: G
authors.lastname: Liu
authors.affiliation: College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo/Biosensing, The Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Laboratory of Molecule-based Materials, Anhui Normal University, Wuhu 241000, PR China.
authors.forename: Wenxiu
authors.initials: W
authors.lastname: Liu
authors.affiliation: College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo/Biosensing, The Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Laboratory of Molecule-based Materials, Anhui Normal University, Wuhu 241000, PR China.
authors.forename: Yiru
authors.initials: Y
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques; D005453:Fluorescence; D006108:Graphite / Q000737:chemistry*; D006493:Heparin / Q000302:isolation & purification*; D010087:Oxides / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry*
chemical_list: D010087:Oxides; D012235:Rhodamines; D006108:Graphite; D011094:Polyethyleneimine; D006493:Heparin; C029773:rhodamine B
keywords: Fluorescent probes; Graphene oxide; Heparin; Rhodamine B