title: Loss of ATP-dependent lysine uptake in the vacuolar membrane vesicles of Saccharomyces cerevisiae ypq1∆ mutant.
abstract: Saccharomyces cerevisiae Ypq1p is a vacuolar membrane protein of the PQ-loop protein family. We found that ATP-dependent uptake activities of amino acids by vacuolar membrane vesicles were impaired by ypq1∆ mutation. Loss of lysine uptake was most remarkable, and the uptake was recovered by overproduction of Ypq1p. Ypq1p is thus involved in transport of amino acids into vacuoles.
authors.affiliation: a Faculty of Agriculture, Department of Applied Bioresource Science , Ehime University , Matsuyama , Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Sekito
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Nakamura
authors.forename: Kunio
authors.initials: K
authors.lastname: Manabe
authors.forename: Junichi
authors.initials: J
authors.lastname: Tone
authors.forename: Yumika
authors.initials: Y
authors.lastname: Sato
authors.forename: Nami
authors.initials: N
authors.lastname: Murao
authors.forename: Miyuki
authors.initials: M
authors.lastname: Kawano-Kawada
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Kakinuma
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D001692:Biological Transport / Q000235:genetics; D007425:Intracellular Membranes / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; C000598342:Ypq1 protein, S cerevisiae; D000255:Adenosine Triphosphate; D008239:Lysine
keywords: PQ-loop; lysine; transport; vacuole