title: Mutagenic effects of ionizing radiation on immature rat oocytes.
abstract: Estimates of genetic risks from radiation delivered to humans are derived largely from mouse studies. In males, the target is spermatogonia and a large amount of information is available. In contrast, in females, immature oocytes are the target, but extrapolations from mice to humans are not very definitive because immature mouse oocytes are highly sensitive to radiation and die by apoptosis, which is not the case in humans. Since mouse offspring derived from surviving immature oocytes have to date not shown any signs of mutation induction, two alternative hypotheses are proposed: 1. Apoptotic death effectively eliminates damaged oocytes in mice and therefore human immature oocytes may be highly mutable; and 2. Immature oocytes are inherently resistant to mutation induction and apoptotic death is not relevant to mutagenesis. To test these hypotheses, rat immature oocytes, which are not as sensitive as those in mice to radiation-induced apoptosis were exposed to 2.5 Gy of gamma rays and the offspring were examined using a two-dimensional DNA analysis method. Screening of a total of 2.26 million DNA fragments, we identified 32 and 18 mutations in the control and exposed groups, respectively. Of these, in the two groups, 29 and 14 mutations were microsatellite mutations, two and one were base changes, and one and three were deletions. Among the four deletions most relevant to radiation exposure, only one was possibly derived from the irradiated dam (but not determined) and three were paternal in origin. Although the number of mutations was small, the results appear to support the second hypothesis and indicate that immature oocytes are generally less sensitive than mature oocytes to mutation induction.
authors.affiliation: a  Department of Genetics, Radiation Effects Research Foundation, Hiroshima, Japan.
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Asakawa
authors.forename: Yujiroh
authors.initials: Y
authors.lastname: Kamiguchi
authors.forename: Kenji
authors.initials: K
authors.lastname: Kamiya
authors.forename: Nori
authors.initials: N
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D004307:Dose-Response Relationship, Radiation; D005260:Female; D008597:Menstrual Cycle / Q000528:radiation effects; D016296:Mutagenesis / Q000528:radiation effects*; D009154:Mutation / Q000528:radiation effects; D009865:Oocytes / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D011839:Radiation, Ionizing; D051381:Rats; D017207:Rats, Sprague-Dawley; D035501:Uncertainty