title: Selection of DNA aptamers against epidermal growth factor receptor with high affinity and specificity.
abstract: Epidermal growth factor receptor (EGFR/HER1/c-ErbB1), is overexpressed in many solid cancers, such as epidermoid carcinomas, malignant gliomas, etc. EGFR plays roles in proliferation, invasion, angiogenesis and metastasis of malignant cancer cells and is the ideal antigen for clinical applications in cancer detection, imaging and therapy. Aptamers, the output of the systematic evolution of ligands by exponential enrichment (SELEX), are DNA/RNA oligonucleotides which can bind protein and other substances with specificity. RNA aptamers are undesirable due to their instability and high cost of production. Conversely, DNA aptamers have aroused researcher's attention because they are easily synthesized, stable, selective, have high binding affinity and are cost-effective to produce. In this study, we have successfully identified DNA aptamers with high binding affinity and selectivity to EGFR. The aptamer named TuTu22 with Kd 56±7.3nM was chosen from the identified DNA aptamers for further study. Flow cytometry analysis results indicated that the TuTu22 aptamer was able to specifically recognize a variety of cancer cells expressing EGFR but did not bind to the EGFR-negative cells. With all of the aforementioned advantages, the DNA aptamers reported here against cancer biomarker EGFR will facilitate the development of novel targeted cancer detection, imaging and therapy.
authors.affiliation: The First Clinical Medical College of Fujian Medical University, Fuzhou, China; Department of Neurosurgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
authors.forename: Deng-Liang
authors.initials: DL
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Chemistry, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Song
authors.affiliation: State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Chemistry, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Chemistry, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Xi-Lan
authors.initials: XL
authors.lastname: Li
authors.affiliation: State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Chemistry, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zou
authors.affiliation: The First Clinical Medical College of Fujian Medical University, Fuzhou, China.
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Yang
authors.affiliation: The First Clinical Medical College of Fujian Medical University, Fuzhou, China.
authors.forename: Jiang-Jie
authors.initials: JJ
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
authors.forename: Pei-Sen
authors.initials: PS
authors.lastname: Yao
authors.affiliation: The First Clinical Medical College of Fujian Medical University, Fuzhou, China.
authors.forename: Ru-Jun
authors.initials: RJ
authors.lastname: Pan
authors.affiliation: State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Chemistry, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China. Electronic address: cyyang@xmu.edu.cn.
authors.forename: Chaoyong James
authors.initials: CJ
authors.lastname: Yang
authors.affiliation: The First Clinical Medical College of Fujian Medical University, Fuzhou, China; Department of Neurosurgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China. Electronic address: kdzy99988@163.com.
authors.forename: De-Zhi
authors.initials: DZ
authors.lastname: Kang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D057054:Molecular Imaging / Q000379:methods; D008969:Molecular Sequence Data; D058990:Molecular Targeted Therapy; D009374:Neoplasms, Experimental / Q000473:pathology / Q000503:physiopathology*; D011485:Protein Binding; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D052157:Aptamers, Nucleotide; C512478:EGFR protein, human; D066246:ErbB Receptors
keywords: Aptamer; DNA; EGFR; SELEX