title: Estrogen decreases anoikis of ovarian cancer cell line Caov-3 through reducing release of Bit1.
abstract: Few studies have referred to the implication of anoikis processes following hormonal treatment. No data are available on the influence of estrogen in ovarian cancer anoikis. To gain insights into the effects and mechanism of estrogen in ovarian cancer cells, we have carried out studies on the anoikis of ovarian cancer cells treated with estrogen and on the pathways involved. We observed an anti-anoikis role of E2 in suspended Caov-3 cells, and this was mainly due to the decreasing of Bit1 level in cytosol. We also found that estrogen receptor α (ERα) was the main mediator involved in this process. To study the signaling pathways well, phosphatidylinositol 3-kinase (PI3K)/AKT were further investigated. Results demonstrated that the decreasing of the Bit1 level in cytosol mediated by E2 binding to ERα was mainly through PI3K/AKT pathways. Overall, these findings disclose a new perspective for estrogen on ovarian cancer therapy.
authors.affiliation: 1 Department of Oncology, Guangzhou General Hospital of Guangzhou Military Command , Guangdong, People's Republic of China .
authors.forename: Jihua
authors.initials: J
authors.lastname: Zheng
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
authors.forename: Xin
authors.initials: X
authors.lastname: Xie
authors.forename: Bo
authors.initials: B
authors.lastname: Xie
authors.forename: Jinrong
authors.initials: J
authors.lastname: Lin
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Xu
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhang
mesh_terms: D023102:Anoikis; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D045744:Cell Line, Tumor; D003600:Cytosol / Q000378:metabolism; D047628:Estrogen Receptor alpha; D004967:Estrogens / Q000502:physiology*; D005260:Female; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism*; D010051:Ovarian Neoplasms; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D002265:Carboxylic Ester Hydrolases; D047628:Estrogen Receptor alpha; D004967:Estrogens; D024101:Mitochondrial Proteins; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; C506487:ESR1 protein, human; C483418:PTH2 protein, human