title: Carrier-inside-carrier: polyelectrolyte microcapsules as reservoir for drug-loaded liposomes.
abstract: Conventional liposomes have a short life-time in blood, unless they are protected by a polymer envelope, most often polyethylene glycol. However, these stabilizing polymers frequently interfere with cellular uptake, impede liposome-membrane fusion and inhibit escape of liposome content from endosomes. To overcome such drawbacks, polymer-based systems as carriers for liposomes are currently developed. Conforming to this approach, we propose a new and convenient method for embedding small size liposomes, 30-100 nm, inside porous calcium carbonate microparticles. These microparticles served as templates for deposition of various polyelectrolytes to form a protective shell. The carbonate particles were then dissolved to yield hollow polyelectrolyte microcapsules. The main advantage of using this method for liposome encapsulation is that carbonate particles can serve as a sacrificial template for deposition of virtually any polyelectrolyte. By carefully choosing the shell composition, bioavailability of the liposomes and of the encapsulated drug can be modulated to respond to biological requirements and to improve drug delivery to the cytoplasm and avoid endosomal escape.
authors.affiliation: ICBMS - UMR CNRS 5246, GEMBAS and ODMB Team, Université de Lyon , Villeurbanne Cedex , France.
authors.forename: Ofelia
authors.initials: O
authors.lastname: Maniti
authors.forename: Samuel
authors.initials: S
authors.lastname: Rebaud
authors.forename: Joe
authors.initials: J
authors.lastname: Sarkis
authors.forename: Yi
authors.initials: Y
authors.lastname: Jia
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.forename: Olivier
authors.initials: O
authors.lastname: Marcillat
authors.forename: Thierry
authors.initials: T
authors.lastname: Granjon
authors.forename: Loïc
authors.initials: L
authors.lastname: Blum
authors.forename: Junbai
authors.initials: J
authors.lastname: Li
authors.forename: Agnès
authors.initials: A
authors.lastname: Girard-Egrot
mesh_terms: D002119:Calcium Carbonate / Q000737:chemistry; D002214:Capsules / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D013499:Surface Properties
chemical_list: D002214:Capsules; D004337:Drug Carriers; D004573:Electrolytes; D008081:Liposomes; D011108:Polymers; D002119:Calcium Carbonate
keywords: Calcium carbonate templates; drug delivery; liposomes; nanotechnology; polyelectrolyte microcapsules; sustained release