title: The use of temporalis muscle graft, fresh and cryopreserved amniotic membrane in preventing temporomandibular joint ankylosis after discectomy in rabbits.
abstract: OBJECTIVE
  The aim of the study was to evaluate the efficacy of temporalis muscle-fascia graft, fresh and cryopreserved human amniotic membrane as an interpositional material in preventing temporomandibular joint ankylosis in a rabbit model.
  
  
  METHODS
  In this experimental study, 21 New Zealand white rabbits were used. The condyle and the joint disc were removed to induce ankylosis in left TMJs. Reconstruction was immediately performed with temporalis muscle-fascia graft (tMFG) in group I (n = 7), fresh human amniotic membrane (fHAM) in group II (n = 7) and cryopreserved human amniotic membrane (cHAM) in group III (n = 7). All rabbits were sacrificed at 3 months after the operation. The comparison was made among three groups by means of vertical mouth opening and weight measurements, radiologic and histologic findings obtained before and after surgery.
  
  
  RESULTS
  In all rabbits, there was no statistically significant difference in the jaw movements and weight among groups at commencement and 3 months after surgery. The condylar surfaces were more irregular in HAM groups. There were mild osteophyte formations, sclerosis, fibrosis and calcification around the condyle in all groups however the joint gap was more preserved in group I. All interpositional materials were also seen to be partially present in the joint gap at 3 months. Ankylosis was not seen in the joint gap in any group.
  
  
  CONCLUSIONS
  With the results of this study it was concluded that interpositional arthroplasty with HAM and tMFG have an almost similar effect in preventing TMJ ankylosis after discectomy in the rabbit model.
authors.affiliation: Department of Plastic Reconstructive and Aesthetic Surgery (Head: Prof. N. Kostakoglu, MD), Gaziosmanpasa University, Faculty of Medicine, 60100 Tokat, Turkey. Electronic address: drumuttuncel@gmail.com.
authors.forename: Umut
authors.initials: U
authors.lastname: Tuncel
authors.affiliation: Department of Plastic Reconstructive and Aesthetic Surgery (Head: Prof. N. Kostakoglu, MD), Gaziosmanpasa University, Faculty of Medicine, 60100 Tokat, Turkey. Electronic address: naci.kostakoglu@gop.edu.tr.
authors.forename: Naci
authors.initials: N
authors.lastname: Kostakoglu
authors.affiliation: Department of Plastic Reconstructive and Aesthetic Surgery (Head: Prof. N. Kostakoglu, MD), Gaziosmanpasa University, Faculty of Medicine, 60100 Tokat, Turkey. Electronic address: aturanprs@yahoo.com.
authors.forename: Aydın
authors.initials: A
authors.lastname: Turan
authors.affiliation: Department of Patology, Gaziosmanpasa University, Faculty of Medicine, 60100 Tokat, Turkey. Electronic address: fatma.markoc@gop.edu.tr.
authors.forename: Fatma
authors.initials: F
authors.lastname: Markoç
authors.affiliation: Department of Radiology, Gaziosmanpasa University, Faculty of Medicine, 60100 Tokat, Turkey. Electronic address: erkan.gokce@gop.edu.tr.
authors.forename: Erkan
authors.initials: E
authors.lastname: Gokçe
authors.affiliation: Department of Biostatistics, Sakarya University, Faculty of Medicine, 60100 Sakarya, Turkey. Electronic address: uerkorkmaz@gmail.com.
authors.forename: Unal
authors.initials: U
authors.lastname: Erkorkmaz
mesh_terms: D000650:Amnion / Q000637:transplantation*; D000818:Animals; D000844:Ankylosis / Q000517:prevention & control*; D001178:Arthroplasty / Q000379:methods; D016723:Bone Remodeling / Q000502:physiology; D002114:Calcinosis / Q000209:etiology; D015925:Cryopreservation / Q000379:methods; D004195:Disease Models, Animal; D005205:Fascia / Q000637:transplantation; D005355:Fibrosis; D006801:Humans; D008335:Mandibular Condyle / Q000473:pathology / Q000601:surgery; D010012:Osteogenesis / Q000502:physiology; D054850:Osteophyte / Q000209:etiology; D010026:Osteosclerosis / Q000209:etiology; D011183:Postoperative Complications; D011817:Rabbits; D011897:Random Allocation; D016059:Range of Motion, Articular / Q000502:physiology; D019651:Plastic Surgery Procedures / Q000379:methods; D013701:Temporal Bone / Q000473:pathology; D013703:Temporal Muscle / Q000637:transplantation*; D019224:Temporomandibular Joint Disc / Q000601:surgery*; D013705:Temporomandibular Joint Disorders / Q000517:prevention & control*
keywords: Amniotic membrane; Ankylosis; Muscle graft; Temporomandibular joint