title: Genome-wide analysis of the MADS-box gene family in Brassica rapa (Chinese cabbage).
abstract: The MADS-box gene family is an ancient and well-studied transcription factor family that functions in almost every developmental process in plants. There are a number of reports about the MADS-box family in different plant species, but systematic analysis of the MADS-box transcription factor family in Brassica rapa (Chinese cabbage) is still lacking. In this study, 160 MADS-box transcription factors were identified from the entire Chinese cabbage genome and compared with the MADS-box factors from 21 other representative plant species. A detailed list of MADS proteins from these 22 species was sorted. Phylogenetic analysis of the BrMADS genes, together with their Arabidopsis and rice counterparts, showed that the BrMADS genes were categorised into type I (Mα, Mβ, Mγ) and type II (MIKC(C), MIKC*) groups, and the MIKC(C) proteins were further divided into 13 subfamilies. The Chinese cabbage type II group has 95 members, which is twice as much as the Arabidopsis type II group, indicating that the Chinese cabbage type II genes have been retained more frequently than the type I genes. Finally, RNA-seq transcriptome data and quantitative real-time PCR analysis revealed that BrMADS genes are expressed in a tissue-specific manner similar to Arabidopsis. Interestingly, a number of BrMIKC genes showed responses to different abiotic stress treatments, suggesting a function for some of the genes in these processes as well. Taken together, the characterization of the B. rapa MADS-box family presented here, will certainly help in the selection of appropriate candidate genes and further facilitate functional studies in Chinese cabbage.
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Weike
authors.initials: W
authors.lastname: Duan
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Song
authors.forename: Tongkun
authors.initials: T
authors.lastname: Liu
authors.forename: Zhinan
authors.initials: Z
authors.lastname: Huang
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.forename: Xilin
authors.initials: X
authors.lastname: Hou
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
mesh_terms: D029741:Brassica rapa / Q000235:genetics*; D032461:Chromosomes, Plant / Q000235:genetics; D017124:Conserved Sequence / Q000235:genetics; D056915:DNA Copy Number Variations / Q000235:genetics; D019143:Evolution, Molecular; D020440:Gene Duplication; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D026161:MADS Domain Proteins / Q000235:genetics*; D005810:Multigene Family; D059372:Nucleotide Motifs / Q000235:genetics; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D026161:MADS Domain Proteins