title: Exploring metabolic pathways and regulation through functional chemoproteomic and metabolomic platforms.
abstract: Genome sequencing efforts have revealed a strikingly large number of uncharacterized genes, including poorly or uncharacterized metabolic enzymes, metabolites, and metabolic networks that operate in normal physiology, and those enzymes and pathways that may be rewired under pathological conditions. Although deciphering the functions of the uncharacterized metabolic genome is a challenging prospect, it also presents an opportunity for identifying novel metabolic nodes that may be important in disease therapy. In this review, we will discuss the chemoproteomic and metabolomic platforms used in identifying, characterizing, and targeting nodal metabolic pathways important in physiology and disease, describing an integrated workflow for functional mapping of metabolic enzymes.
authors.affiliation: Program in Metabolic Biology and Molecular Toxicology, Department of Nutritional Sciences and Toxicology, 127 Morgan Hall, Berkeley, CA 94720, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Medina-Cleghorn
authors.affiliation: Program in Metabolic Biology and Molecular Toxicology, Department of Nutritional Sciences and Toxicology, 127 Morgan Hall, Berkeley, CA 94720, USA. Electronic address: dnomura@berkeley.edu.
authors.forename: Daniel K
authors.initials: DK
authors.lastname: Nomura
mesh_terms: D004798:Enzymes / Q000737:chemistry / Q000378:metabolism; D044127:Epigenesis, Genetic; D006801:Humans; D053858:Metabolic Networks and Pathways; D055432:Metabolomics; D015335:Molecular Probes / Q000737:chemistry / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D040901:Proteomics
chemical_list: D004798:Enzymes; D015335:Molecular Probes