title: Cell surface marker mediated purification of iPS cell intermediates from a reprogrammable mouse model.
abstract: Mature cells can be reprogrammed to a pluripotent state. These so called induced pluripotent stem (iPS) cells are able to give rise to all cell types of the body and consequently have vast potential for regenerative medicine applications. Traditionally iPS cells are generated by viral introduction of transcription factors Oct-4, Klf-4, Sox-2, and c-Myc (OKSM) into fibroblasts. However, reprogramming is an inefficient process with only 0.1-1% of cells reverting towards a pluripotent state, making it difficult to study the reprogramming mechanism. A proven methodology that has allowed the study of the reprogramming process is to separate the rare intermediates of the reaction from the refractory bulk population. In the case of mouse embryonic fibroblasts (MEFs), we and others have previously shown that reprogramming cells undergo a distinct series of changes in the expression profile of cell surface markers which can be used for the separation of these cells. During the early stages of OKSM expression successfully reprogramming cells lose fibroblast identity marker Thy-1.2 and up-regulate pluripotency associated marker Ssea-1. The final transition of a subset of Ssea-1 positive cells towards the pluripotent state is marked by the expression of Epcam during the late stages of reprogramming. Here we provide a detailed description of the methodology used to isolate reprogramming intermediates from cultures of reprogramming MEFs. In order to increase experimental reproducibility we use a reprogrammable mouse strain that has been engineered to express a transcriptional transactivator (m2rtTA) under control of the Rosa26 locus and OKSM under control of a doxycycline responsive promoter. Cells isolated from these mice are isogenic and express OKSM homogenously upon addition of doxycycline. We describe in detail the establishment of the reprogrammable mice, the derivation of MEFs, and the subsequent isolation of intermediates during reprogramming into iPS cells via fluorescent activated cells sorting (FACS).
authors.affiliation: Department of Anatomy and Developmental Biology, Monash University; Australian Regenerative Medicine Institute, Monash University.
authors.forename: Christian M
authors.initials: CM
authors.lastname: Nefzger
authors.affiliation: Department of Anatomy and Developmental Biology, Monash University; Australian Regenerative Medicine Institute, Monash University.
authors.forename: Sara
authors.initials: S
authors.lastname: Alaei
authors.affiliation: Department of Anatomy and Developmental Biology, Monash University; Australian Regenerative Medicine Institute, Monash University.
authors.forename: Anja S
authors.initials: AS
authors.lastname: Knaupp
authors.affiliation: Department of Anatomy and Developmental Biology, Monash University; Australian Regenerative Medicine Institute, Monash University.
authors.forename: Melissa L
authors.initials: ML
authors.lastname: Holmes
authors.affiliation: Department of Anatomy and Developmental Biology, Monash University; Australian Regenerative Medicine Institute, Monash University; Jose.Polo@monash.edu.
authors.forename: Jose M
authors.initials: JM
authors.lastname: Polo
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis / Q000096:biosynthesis; D000954:Antigens, Surface / Q000032:analysis* / Q000096:biosynthesis / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000096:biosynthesis; D004622:Embryo, Mammalian / Q000166:cytology; D000071858:Epithelial Cell Adhesion Molecule; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods*; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D016256:Lewis X Antigen / Q000032:analysis / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D011247:Pregnancy; D018800:Thy-1 Antigens / Q000032:analysis / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis
chemical_list: D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D016256:Lewis X Antigen; D018800:Thy-1 Antigens; D014157:Transcription Factors