title: Live-cell targeting of his-tagged proteins by multivalent N-nitrilotriacetic acid carrier complexes.
abstract: Selective and fast labeling of proteins in living cells is a major challenge. Live-cell labeling techniques require high specificity, high labeling density, and cell permeability of the tagging molecule to target the protein of interest. Here we report on the site-specific, rapid, and efficient labeling of endogenous and recombinant histidine-tagged proteins in distinct subcellular compartments using cell-penetrating multivalent chelator carrier complexes. In vivo labeling was followed in real time in living cells, demonstrating a high specificity and high degree of colocalization in the crowded cellular environment.
authors.affiliation: Institute of Biochemistry, Goethe-University Frankfurt , Max-von-Laue-Str. 9, D-60438 Frankfurt am Main, Germany.
authors.forename: Ralph
authors.initials: R
authors.lastname: Wieneke
authors.forename: Noemi
authors.initials: N
authors.lastname: Labòria
authors.forename: Malini
authors.initials: M
authors.lastname: Rajan
authors.forename: Alina
authors.initials: A
authors.lastname: Kollmannsperger
authors.forename: Francesco
authors.initials: F
authors.lastname: Natale
authors.forename: M Cristina
authors.initials: MC
authors.lastname: Cardoso
authors.forename: Robert
authors.initials: R
authors.lastname: Tampé
mesh_terms: D000595:Amino Acid Sequence; D002470:Cell Survival; D006367:HeLa Cells; D006639:Histidine / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D009571:Nitrilotriacetic Acid / Q000737:chemistry* / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D013194:Staining and Labeling; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000737:chemistry*
chemical_list: D010446:Peptide Fragments; D011994:Recombinant Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus; D006639:Histidine; D009571:Nitrilotriacetic Acid