title: Expression analysis for genes involved in arachidonic acid biosynthesis in Mortierella alpina CBS 754.68.
abstract: The time courses for production of fungal biomass, lipid, phenolic and arachidonic acid (ARA) as well as expression of the genes involved in biosynthesis of ARA and lipid were examined in Mortierella alpina CBS 754.68. A significant increase in the arachidonic acid content in lipids that coincided with reduced levels of lipid was obtained. Reduced gene expression occurred presumably due to the steady reduction of carbon and nitrogen resources. However, these energy resources were inefficiently compensated by the breakdown of the accumulated lipids that in turn, induced up-regulated expression of the candidate genes. The results further indicated that the expression of the GLELO encoding gene is a rate-limiting step in the biosynthesis of ARA in the early growth phase.
authors.affiliation: Department of Food Science and Technology Faculty of Agriculture Tarbiat Modares University Tehran Iran Department of Food Science and Technology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
authors.forename: Hamid-Reza
authors.initials: HR
authors.lastname: Samadlouie
authors.affiliation: Department of Food Science and Technology Faculty of Agriculture Tarbiat Modares University Tehran Iran Department of Food Science and Technology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Hamidi-Esfahani
authors.affiliation: National institute for Genetic Engineering and Biotechnology Tehran Iran National institute for Genetic Engineering and Biotechnology, Tehran, Iran.
authors.forename: Seyed-Mehdi
authors.initials: SM
authors.lastname: Alavi
authors.affiliation: Food Technology Division School of Industrial Technology Universiti Sains Malaysia PulauPinang Malaysia Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia, Pulau, Pinang, Malaysia.
authors.forename: Boshra
authors.initials: B
authors.lastname: Varastegani
mesh_terms: D016718:Arachidonic Acid / Q000096:biosynthesis*; D053898:Biosynthetic Pathways / Q000235:genetics*; D002244:Carbon / Q000378:metabolism; D020869:Gene Expression Profiling; D005800:Genes, Fungal; D020094:Mortierella / Q000235:genetics* / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D016718:Arachidonic Acid; D002244:Carbon; D009584:Nitrogen
keywords: Mortierella alpina; arachidonic acid; gene expression; lipid; real time PCR