title: Stripe-patterned thermo-responsive cell culture dish for cell separation without cell labeling.
abstract: A stripe-patterned thermo-responsive surface is prepared to enable cell separation without labeling. The thermo-responsive surface containing a 3 μm striped pattern exhibits various cell adhesion and detachment properties. A mixture of three cell types is separated on the patterned surface based on their distinct cell-adhesion properties, and the composition of the cells is analyzed by flow cytometry.
authors.affiliation: Institute of Advanced Biomedical Engineering and Science (TWIns), Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kumashiro
authors.forename: Jun
authors.initials: J
authors.lastname: Ishihara
authors.forename: Terumasa
authors.initials: T
authors.lastname: Umemoto
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Itoga
authors.forename: Jun
authors.initials: J
authors.lastname: Kobayashi
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Shimizu
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D000180:Acrylic Resins / Q000138:chemical synthesis / Q000737:chemistry; D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D006367:HeLa Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D013194:Staining and Labeling / Q000295:instrumentation / Q000379:methods; D013499:Surface Properties; D013696:Temperature; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D020099:Coated Materials, Biocompatible; C052970:poly-N-isopropylacrylamide
keywords: biomaterials; cell adhesion; cell cultures; cell separation; label-free; patterning