title: In vitro cytocompatibility evaluation of chitosan/graphene oxide 3D scaffold composites designed for bone tissue engineering.
abstract: Extensively studied nowadays, graphene oxide (GO) has a benefic effect on cell proliferation and differentiation, thus holding promise for bone tissue engineering (BTE) approaches. The aim of this study was not only to design a chitosan 3D scaffold improved with GO for optimal BTE, but also to analyze its physicochemical properties and to evaluate its cytocompatibility and ability to support cell metabolic activity and proliferation. Overall results show that the addition of GO in the scaffold's composition improved mechanical properties and pore formation and enhanced the bioactivity of the scaffold material for tissue engineering. The new developed CHT/GO 3 wt% scaffold could be a potential candidate for further in vitro and in vivo osteogenesis studies and BTE approaches.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania.
authors.forename: Sorina
authors.initials: S
authors.lastname: Dinescu
authors.affiliation: University Politehnica of Bucharest, Calea Victoriei 149, 010072 Bucharest, Romania.
authors.forename: Mariana
authors.initials: M
authors.lastname: Ionita
authors.affiliation: University Politehnica of Bucharest, Calea Victoriei 149, 010072 Bucharest, Romania.
authors.forename: Andreea Madalina
authors.initials: AM
authors.lastname: Pandele
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania Department of Experimental and Applied Biology, Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, 310414 Arad, Romania.
authors.forename: Bianca
authors.initials: B
authors.lastname: Galateanu
authors.affiliation: University Politehnica of Bucharest, Calea Victoriei 149, 010072 Bucharest, Romania.
authors.forename: Horia
authors.initials: H
authors.lastname: Iovu
authors.affiliation: Department of Experimental and Applied Biology, Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, 310414 Arad, Romania.
authors.forename: Aurel
authors.initials: A
authors.lastname: Ardelean
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania.
authors.forename: Marieta
authors.initials: M
authors.lastname: Costache
authors.affiliation: Department of Experimental and Applied Biology, Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, 310414 Arad, Romania.
authors.forename: Anca
authors.initials: A
authors.lastname: Hermenean
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials / Q000737:chemistry*; D018786:Bone Substitutes / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D002522:Chlorocebus aethiops; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry* / Q000494:pharmacology; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology; D011474:Prosthesis Design; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds; D014709:Vero Cells
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D010087:Oxides; D006108:Graphite; D048271:Chitosan
keywords: 3D scaffold; Chitosan; biocompatibility; bone tissue engineering; graphene oxide