title: Synthesis and structure elucidation of a copper(II) Schiff-base complex: in vitro DNA binding, pBR322 plasmid cleavage and HSA binding studies.
abstract: New copper(II) complex with Schiff base ligand 4-[(2-Hydroxy-3-methoxy-benzylidene)-amino]-benzoic acid (H₂L) was synthesized and characterized by spectroscopic and analytical and single crystal X-ray diffraction studies which revealed that the complex 1 exist in a distorted octahedral environment. In vitro CT-DNA binding studies were performed by employing different biophysical technique which indicated that the 1 strongly binds to DNA in comparison to ligand via electrostatic binding mode. Complex 1 cleaves pBR322 DNA via hydrolytic pathway and recognizes minor groove of DNA double helix. The HSA binding results showed that ligand and complex 1 has ability to quench the fluorescence emission intensity of Trp 214 residue available in the subdomain IIA of HSA.
authors.affiliation: Department of Chemistry, Aligarh Muslim University, Aligarh, UP 202002, India. Electronic address: tsartaj62@yahoo.com.
authors.forename: Sartaj
authors.initials: S
authors.lastname: Tabassum
authors.affiliation: Department of Chemistry, Indian Institute of Technology, Kanpur, UP 208016, India.
authors.forename: Musheer
authors.initials: M
authors.lastname: Ahmad
authors.affiliation: Department of Chemistry, Aligarh Muslim University, Aligarh, UP 202002, India.
authors.forename: Mohd
authors.initials: M
authors.lastname: Afzal
authors.affiliation: Department of Chemistry, Aligarh Muslim University, Aligarh, UP 202002, India.
authors.forename: Mehvash
authors.initials: M
authors.lastname: Zaki
authors.affiliation: Department of Chemistry, Indian Institute of Technology, Kanpur, UP 208016, India.
authors.forename: Parimal K
authors.initials: PK
authors.lastname: Bharadwaj
mesh_terms: D000818:Animals; D002417:Cattle; D060326:Chemistry Techniques, Synthetic; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D053837:DNA Cleavage / Q000187:drug effects*; D006801:Humans; D062105:Molecular Docking Simulation; D009690:Nucleic Acid Conformation; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics*; D011487:Protein Conformation; D017382:Reactive Oxygen Species / Q000494:pharmacology; D012545:Schiff Bases / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D055672:Static Electricity
chemical_list: D009942:Organometallic Compounds; D017382:Reactive Oxygen Species; D012545:Schiff Bases; D012709:Serum Albumin; D003300:Copper; D004247:DNA
keywords: HSA binding; In vitro DNA binding; Molecular docking; pBR322 DNA cleavage