title: An optimized extraction technique for acetylcholinesterase inhibitors from the Camellia japonica seed cake by using response surface methodology.
abstract: The response surface methodology (RSM) was used to optimize the extraction conditions for the acetylcholinesterase (AchE) inhibitory activity and extraction yield from Camellia japonica seed cake. Predicted values for AchE inhibition and extraction yield were 19.41 and 13.35%, respectively, which are in good agreement with the experimental values from validation, suggesting that RSM may provide a useful tool to optimization processes.
authors.affiliation: a Department of Food Science and Nutrition , University of Minnesota , St. Paul , MN , USA.
authors.forename: Jae Kyeom
authors.initials: JK
authors.lastname: Kim
authors.forename: Cho Rong
authors.initials: CR
authors.lastname: Kim
authors.forename: Ho-Jeong
authors.initials: HJ
authors.lastname: Lim
authors.forename: Sang Hae
authors.initials: SH
authors.lastname: Nam
authors.forename: Ok Soo
authors.initials: OS
authors.lastname: Joo
authors.forename: Dong-Hoon
authors.initials: DH
authors.lastname: Shin
authors.forename: Eui-Cheol
authors.initials: EC
authors.lastname: Shin
mesh_terms: D028244:Camellia / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D000431:Ethanol / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
chemical_list: D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; D000431:Ethanol
keywords: Alzheimer’s disease; Camellia japonica; acetylcholinesterase; camellia seed cake; response surface methodology