title: High-throughput sequencing of plasma microRNA in chronic fatigue syndrome/myalgic encephalomyelitis.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are known to regulate many biological processes and their dysregulation has been associated with a variety of diseases including Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME). The recent discovery of stable and reproducible miRNA in plasma has raised the possibility that circulating miRNAs may serve as novel diagnostic markers. The objective of this study was to determine the role of plasma miRNA in CFS/ME.
  
  
  RESULTS
  Using Illumina high-throughput sequencing we identified 19 miRNAs that were differentially expressed in the plasma of CFS/ME patients in comparison to non-fatigued controls. Following RT-qPCR analysis, we were able to confirm the significant up-regulation of three miRNAs (hsa-miR-127-3p, hsa-miR-142-5p and hsa-miR-143-3p) in the CFS/ME patients.
  
  
  CONCLUSIONS
  Our study is the first to identify circulating miRNAs from CFS/ME patients and also to confirm three differentially expressed circulating miRNAs in CFS/ME patients, providing a basis for further study to find useful CFS/ME biomarkers.
authors.affiliation: School of Medical Science, Griffith Health Centre, Griffith University, Gold Coast, Queensland, Australia; The National Centre for Neuroimmunology and Emerging Diseases, Griffith University, Gold Coast, Queensland, Australia.
authors.forename: Ekua W
authors.initials: EW
authors.lastname: Brenu
authors.affiliation: Faculty of Health Sciences and Medicine, Bond University, Robina, Queensland, Australia.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Ashton
authors.affiliation: Faculty of Health Sciences and Medicine, Bond University, Robina, Queensland, Australia.
authors.forename: Jana
authors.initials: J
authors.lastname: Batovska
authors.affiliation: The National Centre for Neuroimmunology and Emerging Diseases, Griffith University, Gold Coast, Queensland, Australia; Queensland Health, Gold Coast Public Health Unit, Robina, Gold Coast, Queensland, Australia.
authors.forename: Donald R
authors.initials: DR
authors.lastname: Staines
authors.affiliation: School of Medical Science, Griffith Health Centre, Griffith University, Gold Coast, Queensland, Australia; The National Centre for Neuroimmunology and Emerging Diseases, Griffith University, Gold Coast, Queensland, Australia.
authors.forename: Sonya M
authors.initials: SM
authors.lastname: Marshall-Gradisnik
mesh_terms: D000328:Adult; D015673:Fatigue Syndrome, Chronic / Q000235:genetics*; D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs