title: [Parasitological study of 78 cases of human cystic echinococcosis collected between 2005 to 2012 in Mustapha hospital center of Algiers].
abstract: OBJECTIVE
  The aim of this work is to know the fertility rate of the metacestodes resulting from patients suffering from hydatidosis, the one of protoscoleces's viability and by comparing the results obtained with those found elsewhere. It reports, also, the epidemiological, clinical and diagnostically aspects of the studied patients.
  
  
  METHODS
  This study has carried on 78 hydatics samples resulting from 78 patients collected between 2005 and 2012 at the laboratory of parasitology of the Mustapha hospital center of Algiers. A questionnaire on the epidemiological context (contact with an animal-host of the cycle, place of residence, presence of family cases reached of hydatidosis and knowledge on the hydatic disease) concerned 69 patients. For each sample, a direct microscopic examination is made with or without vital staining. The presence of protoscoleces made qualified the fertile cyst. Those visualized moving or resistant to eosin at 0.2% are considered viables. Indirect diagnosis is based on the techniques: passive hemagglutination, electrophoresis, Elisa IgG Echinococcus granulosus and immunoblotting IgG "Echinococcus". Molecular analysis is based on PCR and sequencing the partials fragments of two mitochondrial genes with the primers COX1 and ND1.
  
  
  RESULTS
  The results obtained show that the surgical frequency of hydatidosis is significant at the young adult and at the child. The epidemiological context associated at the disease is the conjointly presence of a dog and herbivores. The fertility rate of human hydatid cysts is 88.4% and the ones of viability of the protoscoleces is 74.5%. In this series, the serology shows global positivity at 70%. The molecular characterization of five samples identify the species: E. granulosus ss.
  
  
  CONCLUSIONS
  Finally, the viability and fertility rates found here are raised. Sometimes viables protoscoleses are found after use of scolicidal solution. In front of these results, the parasitical treatment is more than necessary in order to minimize the risk of occurred of secondary echinococcosis or the relapses postoperatives.
authors.affiliation: Laboratoire de parasitologie-mycologie, CHU Mustapha, place du 1(er) mai, 16000 Alger, Algérie. Electronic address: houria.zait@yahoo.fr.
authors.forename: H
authors.initials: H
authors.lastname: Zait
authors.affiliation: Laboratoire de parasitologie-mycologie, CHU Mustapha, place du 1(er) mai, 16000 Alger, Algérie.
authors.forename: M
authors.initials: M
authors.lastname: Boulahbel
authors.affiliation: Service de parasitologie-mycologie, hôpital la Timone, 13385 Marseille cedex 05, France.
authors.forename: A C
authors.initials: AC
authors.lastname: Normand
authors.affiliation: Service de chirurgie infantile, hôpital militaire HCA, Ain naadja, 16000 Alger, Algérie.
authors.forename: F
authors.initials: F
authors.lastname: Zait
authors.affiliation: Laboratoire de parasitologie-mycologie, CHU Mustapha, place du 1(er) mai, 16000 Alger, Algérie.
authors.forename: I
authors.initials: I
authors.lastname: Achir
authors.affiliation: Service d'épidémiologie et médecine préventive (SEMEP), CHU Mustapha, place du 1(er) mai, 16000 Alger, Algérie.
authors.forename: M K
authors.initials: MK
authors.lastname: Guerchani
authors.affiliation: Service de chirurgie thoracique, CHU Mustapha, place du 1(er) mai, 16000 Alger, Algérie.
authors.forename: H
authors.initials: H
authors.lastname: Chaouche
authors.affiliation: Service de chirurgie infantile, CHU Mustapha, place du 1(er) mai, 16000 Alger, Algérie.
authors.forename: Y
authors.initials: Y
authors.lastname: Ladjadj
authors.affiliation: Laboratoire de parasitologie-mycologie, CHU Mustapha, place du 1(er) mai, 16000 Alger, Algérie.
authors.forename: B
authors.initials: B
authors.lastname: Hamrioui
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000462:Algeria / Q000453:epidemiology; D000818:Animals; D000829:Animals, Domestic; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D004285:Dogs; D004443:Echinococcosis / Q000453:epidemiology* / Q000469:parasitology*; D005260:Female; D006785:Hospitals, University; D006801:Humans; D008297:Male; D008875:Middle Aged; D055815:Young Adult
keywords: Algeria; Algérie; Contexte épidémiologique; Echinococcose kystique humaine; Epidemiological context; Fertility; Fertilité; Human cystic echinococcosis; Protoscoleses; Protoscolex; Viability; Viabilité