title: Towards an in vitro model mimicking the foreign body response: tailoring the surface properties of biomaterials to modulate extracellular matrix.
abstract: Despite various studies to minimize host reaction following a biomaterial implantation, an appealing strategy in regenerative medicine is to actively use such an immune response to trigger and control tissue regeneration. We have developed an in vitro model to modulate the host response by tuning biomaterials' surface properties through surface modifications techniques as a new strategy for tissue regeneration applications. Results showed tunable surface topography, roughness, wettability, and chemistry by varying treatment type and exposure, allowing for the first time to correlate the effect of these surface properties on cell attachment, morphology, strength and proliferation, as well as proinflammatory (IL-1β, IL-6) and antiinflammatory cytokines (TGF-β1, IL-10) secreted in medium, and protein expression of collagen and elastin. Surface microstructuring, derived from chloroform partial etching, increased surface roughness and oxygen content. This resulted in enhanced cell adhesion, strength and proliferation as well as a balance of soluble factors for optimum collagen and elastin synthesis for tissue regeneration. By linking surface parameters to cell activity, we could determine the fate of the regenerated tissue to create successful soft tissue-engineered replacement.
authors.affiliation: University of Twente, Drienerlolaan 5, Zuidhorst 145, 7522 NB Enschede, the Netherlands.
authors.forename: Febriyani F R
authors.initials: FF
authors.lastname: Damanik
authors.affiliation: Department of Nephrology, Leiden University Medical Center, Albinusdreef 2, 2333ZA Leiden, The Netherlands.
authors.forename: Tonia C
authors.initials: TC
authors.lastname: Rothuizen
authors.affiliation: 1] University of Twente, Drienerlolaan 5, Zuidhorst 145, 7522 NB Enschede, the Netherlands [2] Maastricht University, MERLN Institute for Technology Inspired Regenerative Medicine, Complex Tissue Regeneration, PO Box 616, 6200 MD Maastricht, The Netherlands.
authors.forename: Clemens
authors.initials: C
authors.lastname: van Blitterswijk
authors.affiliation: Department of Nephrology, Leiden University Medical Center, Albinusdreef 2, 2333ZA Leiden, The Netherlands.
authors.forename: Joris I
authors.initials: JI
authors.lastname: Rotmans
authors.affiliation: 1] University of Twente, Drienerlolaan 5, Zuidhorst 145, 7522 NB Enschede, the Netherlands [2] Maastricht University, MERLN Institute for Technology Inspired Regenerative Medicine, Complex Tissue Regeneration, PO Box 616, 6200 MD Maastricht, The Netherlands.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Moroni
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000235:genetics; D003094:Collagen / Q000096:biosynthesis; D004549:Elastin / Q000096:biosynthesis; D005109:Extracellular Matrix / Q000187:drug effects* / Q000276:immunology; D005547:Foreign Bodies / Q000276:immunology*; D005786:Gene Expression Regulation / Q000187:drug effects; D007110:Immunity, Active / Q000235:genetics*; D066298:In Vitro Techniques; D016753:Interleukin-10 / Q000096:biosynthesis; D053583:Interleukin-1beta / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D051381:Rats; D044968:Regenerative Medicine; D013499:Surface Properties; D023822:Tissue Engineering; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis / Q000235:genetics
chemical_list: D001672:Biocompatible Materials; D053583:Interleukin-1beta; D015850:Interleukin-6; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10; D003094:Collagen; D004549:Elastin