title: Selective determination of trace 17β-estradiol in dairy and meat samples by molecularly imprinted solid-phase extraction and HPLC.
abstract: Molecularly imprinted solid-phase extraction (MISPE) combined with HPLC were used to detect trace 17β-estradiol (E2) in different dairy and meat samples. E2 imprinted mono-dispersed microspheres prepared by modified precipitation polymerisation method were used as MISPE sorbents. Under optimum MISPE and HPLC conditions, trace (0.116-0.461nmolkg(-1)) E2 in different dairy and meat samples can be detected accurately using UV detector when large amount of sample (500g) was analysed. Recoveries of E2 from spiked (0.1-5nmolkg(-1)) milk, yogurt, beef, pork and chicken were higher than 74.18%, 71.92%, 67.21%, 64.20% and 65.93%, respectively with RSDs less than 8.38%. MISPE can enrich E2 selectively, which is helpful to have better HPLC separation and higher recoveries than C18 SPE. MISPE combined with HPLC-UV are reliable methods to determine trace E2 in dairy and meat samples with high accuracy and repeatability.
authors.affiliation: MOE Key Laboratory of Environment & Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Shi
authors.affiliation: MOE Key Laboratory of Environment & Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China.
authors.forename: Dong-Dong
authors.initials: DD
authors.lastname: Peng
authors.affiliation: Hubei Entry-Exit Inspection and Quarantine Bureau of PRC, Wuhan, Hubei 430022, China.
authors.forename: Chang-Hua
authors.initials: CH
authors.lastname: Shi
authors.affiliation: Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: MOE Key Laboratory of Environment & Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China.
authors.forename: Ya-Ting
authors.initials: YT
authors.lastname: Xie
authors.affiliation: MOE Key Laboratory of Environment & Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China. Electronic address: lubin@mails.tjmu.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Lu
keywords: Dairy and meat samples; HPLC-UV; MISPE; Modified precipitation polymerisation; Molecularly imprinted microspheres; Trace 17β-estradiol