title: Anti-inflammatory and analgesic effects of the marine-derived compound comaparvin isolated from the crinoid Comanthus bennetti.
abstract: To date, no study has been conducted to explore the bioactivity of the crinoid Comanthus bennetti. Here we report the anti-inflammatory properties of comaparvin (5,8-dihydroxy-10-methoxy-2-propylbenzo[h]chromen-4-one) based on in vivo experiments. Our preliminary screening for anti-inflammatory activity revealed that the crude extract of Comanthus bennetti significantly inhibited the expression of pro-inflammatory proteins in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophage cells. Comaparvin isolated from crinoids significantly decreased the expression of inducible nitric oxide synthase (iNOS) protein and mRNA in LPS-stimulated macrophage cells. Moreover, our results showed that post-treatment with comaparvin significantly inhibited mechanical allodynia, thermal hyperalgesia and weight-bearing deficits in rats with carrageenan-induced inflammation. Comaparvin also attenuated leukocyte infiltration and iNOS protein expression in carrageenan-induced inflamed paws. These results suggest that comaparvin is a potential anti-inflammatory therapeutic agent against inflammatory pain.
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. pharmacy@mail.ngh.com.tw.
authors.forename: Li-Chai
authors.initials: LC
authors.lastname: Chen
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. snakefoot5052@gmail.com.
authors.forename: Yen-You
authors.initials: YY
authors.lastname: Lin
authors.affiliation: Section of Orthopedic Surgery, Pingtung Christian Hospital, Pingtung 90059, Taiwan. jean.tang@msa.hinet.net.
authors.forename: Yen-Hsuan
authors.initials: YH
authors.lastname: Jean
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. snakefoot5052@gmail.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Neurosurgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 83301, Taiwan. ma4949@cgmh.org.tw.
authors.forename: Wu-Fu
authors.initials: WF
authors.lastname: Chen
authors.affiliation: School of Medicine, College of Medicine and Department of Pediatrics, E-DA Hospital, I-Shou University, Kaohsiung 84001, Taiwan. y520729@gmail.com.
authors.forename: San-Nan
authors.initials: SN
authors.lastname: Yang
authors.affiliation: Department of Fragrance and Cosmetic Science, Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. davidw@kmu.edu.tw.
authors.forename: Hui-Min David
authors.initials: HM
authors.lastname: Wang
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. hawkiyc@gmail.com.
authors.forename: Ing-Yang
authors.initials: IY
authors.lastname: Jang
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. 99001@mail.wzu.edu.tw.
authors.forename: I-Ming
authors.initials: IM
authors.lastname: Chen
authors.affiliation: Graduate Institute of Marine Biotechnology, National Dong Hwa University, Pingtung 94450, Taiwan. x2219@nmmba.gov.tw.
authors.forename: Jui-Hsin
authors.initials: JH
authors.lastname: Su
authors.affiliation: Graduate Institute of Marine Biotechnology, National Dong Hwa University, Pingtung 94450, Taiwan. pjsung@nmmba.gov.tw.
authors.forename: Ping-Jyun
authors.initials: PJ
authors.lastname: Sung
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. sheu@mail.nsysu.edu.tw.
authors.forename: Jyh-Horng
authors.initials: JH
authors.lastname: Sheu
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. wzh@mail.nsysu.edu.tw.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Wen
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000737:chemistry; D002351:Carrageenan / Q000633:toxicity; D002867:Chromones / Q000008:administration & dosage* / Q000737:chemistry*; D004447:Echinodermata / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics; D007962:Leukocytes / Q000187:drug effects / Q000473:pathology; D008070:Lipopolysaccharides / Q000633:toxicity; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis*; D051381:Rats
chemical_list: C000597663:5,8-dihydroxy-10-methoxy-2-propylbenzo(h)chromen-4-one; D000893:Anti-Inflammatory Agents; D002867:Chromones; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D002351:Carrageenan; D052247:Nitric Oxide Synthase Type II