title: Knockdown of peroxiredoxin 5 inhibits the growth of osteoarthritic chondrocytes via upregulating Wnt/β-catenin signaling.
abstract: Peroxiredoxin 5 is a member of the peroxiredoxin family, which has been shown to act as an antioxidant whose main function is to reduce reactive oxygen species in cells. Peroxiredoxin 5 has been found to be abnormally elevated in human osteoarthritic chondrocytes. However, the detailed mechanism by which peroxiredoxin 5 modulates human osteoarthritic chondrocytes' survival has not been elucidated. In the current study, we demonstrated that peroxiredoxin 5 knockdown activated osteoarthritic chondrocytes apoptosis, and decreased scavenging of endogenous reactive oxygen species. Furthermore, silencing of peroxiredoxin 5 resulted in an altered expression of proteins associated with Wnt signaling. Collectively, these results demonstrated that the regulatory effects of peroxiredoxin 5 can be partially attributed to Wnt/β-catenin signaling.
authors.affiliation: Department of Combination of Chinese and Western Medicine, The First Affiliated Hospital of Chongqing Medical University, You Yi Road 1#, Chongqing 400016, People's Republic of China.
authors.forename: Yini
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Combination of Chinese and Western Medicine, The First Affiliated Hospital of Chongqing Medical University, You Yi Road 1#, Chongqing 400016, People's Republic of China. Electronic address: wenxiaoyun2011@hotmail.com.
authors.forename: Rongheng
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Combination of Chinese and Western Medicine, The First Affiliated Hospital of Chongqing Medical University, You Yi Road 1#, Chongqing 400016, People's Republic of China.
authors.forename: Yudi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Combination of Chinese and Western Medicine, The First Affiliated Hospital of Chongqing Medical University, You Yi Road 1#, Chongqing 400016, People's Republic of China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Combination of Chinese and Western Medicine, The First Affiliated Hospital of Chongqing Medical University, You Yi Road 1#, Chongqing 400016, People's Republic of China.
authors.forename: Yehong
authors.initials: Y
authors.lastname: Dai
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D016022:Case-Control Studies; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D053509:Matrix Metalloproteinase 13 / Q000235:genetics / Q000378:metabolism; D010003:Osteoarthritis / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D054464:Peroxiredoxins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D015854:Up-Regulation; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism*; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species; D051153:Wnt Proteins; D051176:beta Catenin; C515974:PRDX5 protein, human; D054464:Peroxiredoxins; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13
keywords: Apoptosis; Cartilage; Chondrocytes; Osteoarthritis; Peroxiredoxin 5; Reactive oxygen species