title: Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH.
abstract: Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long non-coding RNAs being identified in the current research. With probes either chemically or biologically synthesized, we demonstrate that the method can be applied to study a wide range of RNA and DNA targets at the single-cell and single-molecule level in cellular contexts.
authors.affiliation: School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
authors.forename: Reelina
authors.initials: R
authors.lastname: Basu
authors.affiliation: School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
authors.forename: Lan-Tian
authors.initials: LT
authors.lastname: Lai
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore, Singapore.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Meng
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore, Singapore.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore, Singapore.
authors.forename: Fangwei
authors.initials: F
authors.lastname: Shao
authors.affiliation: School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D004247:DNA / Q000235:genetics; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D051379:Mice; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry; D012313:RNA / Q000235:genetics; D016615:Telomere / Q000235:genetics
chemical_list: D015345:Oligonucleotide Probes; D012313:RNA; D004247:DNA