title: Angiogenesis-related prognosis in patients with oral squamous cell carcinoma-role of the VEGF +936 C/T polymorphism.
abstract: BACKGROUND
  The aim of the study was the immunohistological assessment of VEGF-single nucleotide polymorphism (SNP)-related angiogenic activity in oral squamous cell carcinoma (OSCC) in correlation with prognosis.
  
  
  METHODS
  Fifty OSCC samples were immunostained with CD31-antibodies. Mean microvessel density (MVD) and staining intensity were determined and associated with clinicopathological/prognostic features as well as with the VEGF +936C/T SNP.
  
  
  RESULTS
  A significant higher MVD could be seen for T3 and T4 compared with T1 and T2, N > 0 vs. N0 as well as G3-G4 vs. G1-G2 OSCCs (all: P < 0.05). A higher MVD was also associated with increased and earlier rates of local relapses, more metastases, and a significant decreased overall as well as disease-free survival (all: P < 0.05). When comparing T1 and T2 samples with +936-T-allele with T 1&2 samples without this allele, staining intensity was significantly increased (P = 0.002).
  
  
  CONCLUSIONS
  Angiogenesis influences local as well as distant growth of OSCCs with a significant correlation between prognostic parameters. The correlation between VEGF +936-T-allele and increased CD31 immunostain needs further confirmation.
authors.affiliation: Department of Oral, Maxillofacial and Plastic Surgery, University Medical Centre of Rostock, Rostock, Germany.
authors.forename: Peer W
authors.initials: PW
authors.lastname: Kämmerer
authors.affiliation: Department of Oral, Maxillofacial and Plastic Surgery, University Medical Centre of the Johannes Gutenberg University Mainz, Mainz, Germany.
authors.forename: Bilal
authors.initials: B
authors.lastname: Al-Nawas
authors.affiliation: Department of Oral, Maxillofacial and Plastic Surgery, University Medical Centre of the Johannes Gutenberg University Mainz, Mainz, Germany.
authors.forename: Sasa
authors.initials: S
authors.lastname: Kalkan
authors.affiliation: Department of Oral, Maxillofacial and Plastic Surgery, University Medical Centre of Rostock, Rostock, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Liese
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Centre of the Johannes Gutenberg University Mainz, Mainz, Germany.
authors.forename: Kai
authors.initials: K
authors.lastname: Fruth
authors.affiliation: Department of Oral, Maxillofacial and Plastic Surgery, University Medical Centre of Rostock, Rostock, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Frerich
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Molecular Tumor Biology Laboratory, University Medical Centre of the Johannes Gutenberg University Mainz, Mainz, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Brieger
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000098:blood supply* / Q000235:genetics* / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D006258:Head and Neck Neoplasms / Q000098:blood supply* / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000098:blood supply* / Q000235:genetics* / Q000473:pathology; D009364:Neoplasm Recurrence, Local / Q000098:blood supply / Q000235:genetics; D016609:Neoplasms, Second Primary / Q000098:blood supply / Q000235:genetics / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D000077195:Squamous Cell Carcinoma of Head and Neck; D015996:Survival Rate; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: CD31; VEGF polymorphism; microvessel density; oral squamous cell carcinoma; prognosis