title: Immunohistochemical evaluation of proliferation, apoptosis and steroidogenic enzymes in the ovary of rats with polycystic ovary.
abstract: to evaluate the immunohistochemical expression of proliferative, apoptotic and steroidogenic enzyme markers in the ovaries of rats with polycystic ovary syndrome (PCOS).
  
  
  
  twenty rats were divided into two groups: GCtrl - estrous phase, and PCOS - with polycystic ovaries. The GCtrl animals were subjected to a lighting period from 7 am to 7 pm, while the animals with PCOS group remained with continuous lighting for 60 days. Subsequently, the animals were anesthetized, the ovaries were removed and fixed in 10% formaldehyde, prior to paraffin embedding. Sections were stained using H.E. or subjected to immunohistochemical methods for the detection of Ki-67, cleaved caspase-3, CYP11A1, CYP17A1 and CYP19A1. The results were analyzed using Student's t-test (p < 0,05).
  
  
  
  morphological results showed evidence of interstitial cells originating from the inner theca cells of degenerating ovarian cysts in PCOS. Immunoexpression of Ki-67 was higher in the granulosa cells in GCtrl, and the theca interna cells in PCOS, while cleaved caspase-3 was higher in granulosa cells of ovarian cysts from PCOS and in the theca interna cells of GCtrl. Immunoreactivity of CYP11A1 in the theca interna, granulosa and interstitial cells was similar between the two groups, while CYP17A1 and CYP19A1 were higher in the granulosa and interstitial cells in the PCOS group.
  
  
  
  the results indicate that the interstitial cells are derived from the theca interna and that enzymatic changes occur in the theca interna and interstitial cells in ovaries of rats with PCOS, responsible for the high levels of androgens and estradiol.
authors.affiliation: Morphology and Genetics Department, Paulista Medical School, Federal University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Leonardo Augusto
authors.initials: LA
authors.lastname: Lombardi
authors.affiliation: Obstetrics and Gynecology Department, Medical School, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Ricardo Santos
authors.initials: RS
authors.lastname: Simões
authors.affiliation: Morphology and Genetics Department, Paulista Medical School, Federal University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Carla Cristina
authors.initials: CC
authors.lastname: Maganhin
authors.affiliation: Obstetrics and Gynecology Department, Medical School, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Maria Cândida Pinheiro
authors.initials: MC
authors.lastname: Baracat
authors.affiliation: Morphology and Genetics Department, Paulista Medical School, Federal University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Gisela Rodrigues
authors.initials: GR
authors.lastname: Silva-Sasso
authors.affiliation: Morphology and Genetics Department, Paulista Medical School, Federal University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Rinaldo
authors.initials: R
authors.lastname: Florencio-Silva
authors.affiliation: Obstetrics and Gynecology Department, Medical School, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: José Maria
authors.initials: JM
authors.lastname: Soares
authors.affiliation: Obstetrics and Gynecology Department, Medical School, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Edmund Chada
authors.initials: EC
authors.lastname: Baracat
mesh_terms: D000818:Animals; D017209:Apoptosis; D015415:Biomarkers / Q000032:analysis; D049109:Cell Proliferation; D005260:Female; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D010053:Ovary / Q000201:enzymology / Q000473:pathology; D011085:Polycystic Ovary Syndrome / Q000201:enzymology* / Q000473:pathology*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D051381:Rats
chemical_list: D015415:Biomarkers; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen