title: Development of slide ELISA (SELISA) for detection of four poultry viral pathogens by direct heat fixation of viruses on glass slides.
abstract: The development of an easy and simpler method of slide enzyme-linked immunosorbent assay (SELISA) for the diagnosis of four economically important poultry viruses viz., Newcastle disease virus (NDV), infectious bronchitis virus (IBV), infectious bursal disease virus (IBDV) and egg drop syndrome 76 virus (EDS 76) and the use of SELISA for semi quantitation of NDV are described. The positive signals for viral aggregates were detected under light microscope. This is the first report regarding the development of SELISA based on heat fixation for the diagnosis of viral pathogens.
authors.affiliation: Avian Diseases Section, Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
authors.forename: P A
authors.initials: PA
authors.lastname: Desingu
authors.affiliation: Avian Diseases Section, Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
authors.forename: S D
authors.initials: SD
authors.lastname: Singh
authors.affiliation: Avian Diseases Section, Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India. Electronic address: kdhama@rediffmail.com.
authors.forename: K
authors.initials: K
authors.lastname: Dhama
authors.affiliation: Division of Medicine, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
authors.forename: O R Vinodh
authors.initials: OR
authors.lastname: Kumar
authors.affiliation: Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
authors.forename: R
authors.initials: R
authors.lastname: Singh
authors.affiliation: Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Singh
mesh_terms: D000818:Animals; D004267:DNA Viruses / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005898:Glass; D006358:Hot Temperature; D008853:Microscopy; D011200:Poultry; D011201:Poultry Diseases / Q000175:diagnosis* / Q000821:virology*; D012328:RNA Viruses / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods*; D014730:Veterinary Medicine / Q000379:methods*; D014773:Virology / Q000379:methods; D014777:Virus Diseases / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology
keywords: Poultry viruses; Semi quantitation; Slide ELISA