title: Downregulation of miR-193a-5p correlates with lymph node metastasis and poor prognosis in colorectal cancer.
abstract: OBJECTIVE
  To investigate the correlation of miR-193a-5p with lymph node metastasis and postoperative survival of colorectal cancer (CRC) patients.
  
  
  METHODS
  A total of 304 formalin-fixed, paraffin-embedded specimens (69 paired cancer and normal tissues, 55 primary tumors of stage III CRC and matched lymph nodes, and 56 primary tumors of stage II CRC) were included in this study. The relative expression levels of miR-193a-5p in the normal mucosa, primary cancer, and metastatic lymph node lesions were measured by quantitative real-time reverse transcriptase polymerase chain reaction. We evaluated the association of its expression with colorectal cancer lymph node metastasis, clinicopathological factors, and patient survival.
  
  
  RESULTS
  The relative expression level of miR-193a-5p was significantly lower in CRC tissues than in the normal mucosa (P = 0.0060). The expression levels of miR-193a-5p were lower in primary CRC tissues with lymph node metastases than in those without metastases (P = 0.0006), and decreased expression of miR-193a-5p correlated with advanced lymph node metastatic stage (P = 0.0007). Kaplan-Meier analysis showed that patients with low miR-193a-5p expression had decreased disease-free survival (DFS) (P = 0.0026) and poor overall survival (OS) (P = 0.0003). Interestingly, for the group of patients with lymph node metastases, miR-193a-5p expression was also related to survival. Patients with low miR-193a-5p expression had decreased DFS (P = 0.0262) and poor OS (P = 0.0230). Moreover, multivariate analysis indicated that downregulation of miR-193a-5p was an independent predictor of poor OS.
  
  
  CONCLUSIONS
  Downregulation of miR-193a-5p correlates with lymph node metastasis and poor survival of CRC. miR-193a-5p may be a useful biomarker for CRC diagnosis, metastasis and prognosis prediction.
authors.affiliation: Peng Zhang, Deng-Bo Ji, Chang-Zheng Du, Jin Gu, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Colorectal Surgery, Peking University Cancer Hospital and Institute, Beijing 100142, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Peng Zhang, Deng-Bo Ji, Chang-Zheng Du, Jin Gu, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Colorectal Surgery, Peking University Cancer Hospital and Institute, Beijing 100142, China.
authors.forename: Deng-Bo
authors.initials: DB
authors.lastname: Ji
authors.affiliation: Peng Zhang, Deng-Bo Ji, Chang-Zheng Du, Jin Gu, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Colorectal Surgery, Peking University Cancer Hospital and Institute, Beijing 100142, China.
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Han
authors.affiliation: Peng Zhang, Deng-Bo Ji, Chang-Zheng Du, Jin Gu, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Colorectal Surgery, Peking University Cancer Hospital and Institute, Beijing 100142, China.
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Shi
authors.affiliation: Peng Zhang, Deng-Bo Ji, Chang-Zheng Du, Jin Gu, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Colorectal Surgery, Peking University Cancer Hospital and Institute, Beijing 100142, China.
authors.forename: Chang-Zheng
authors.initials: CZ
authors.lastname: Du
authors.affiliation: Peng Zhang, Deng-Bo Ji, Chang-Zheng Du, Jin Gu, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Colorectal Surgery, Peking University Cancer Hospital and Institute, Beijing 100142, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Gu
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D016009:Chi-Square Distribution; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D018572:Disease-Free Survival; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D035683:MicroRNAs / Q000235:genetics*; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D013997:Time Factors
chemical_list: D014408:Biomarkers, Tumor; C546818:MIRN193 microRNA, human; D035683:MicroRNAs
keywords: Biomarker; Colorectal cancer; Lymph node metastasis; Prognosis; miR-193a-5p