title: Determination of 3,4-dihydroxyphenylglycol, hydroxytyrosol and tyrosol purified from olive oil by-products with HPLC in animal plasma and tissues.
abstract: A simple, fast and reliable method to quantify, simultaneously, 3,4-dihydroxyphenylglycol (DHPG), hydroxytyrosol (HT) and tyrosol (Ty) extracted and purified successfully from olive oil by-product, called alperujo, in animal plasma and tissues samples has been developed using a high-performance liquid chromatographic (HPLC) method with UV-Vis detection. Extraction of compounds is based on solid-phase extraction for plasma and homogenisation with zirconia beads and centrifugation for tissues. Calibration curves were linear for all three phenols at a relatively low concentration range (0.05-50μg/mL). This method has acceptable accuracy (91-95% in plasma and 63-100% in tissues), precision (1.11-8.26% intra-day and 0.32-9.5% inter-day) and sensitivity for detecting low concentrations of these phenols in small plasma volumes and several animal tissues such as liver, heart, kidney, muscle, testes, white adipose tissue (WAT) and brain.
authors.affiliation: University of Aberdeen, Rowett Institute of Nutrition & Health, Aberdeen, UK. Electronic address: guirogu@cica.es.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Rodríguez-Gutiérrez
authors.affiliation: University of Aberdeen, Rowett Institute of Nutrition & Health, Aberdeen, UK.
authors.forename: Sharon
authors.initials: S
authors.lastname: Wood
authors.affiliation: Instituto de la Grasa (CSIC), Sevilla, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Fernández-Bolaños Guzmán
authors.affiliation: University of Aberdeen, Rowett Institute of Nutrition & Health, Aberdeen, UK.
authors.forename: Garry G
authors.initials: GG
authors.lastname: Duthie
authors.affiliation: University of Aberdeen, Rowett Institute of Nutrition & Health, Aberdeen, UK.
authors.forename: Baukje
authors.initials: B
authors.lastname: de Roos
keywords: 3,4-Dihydroxyphenylglycol; Alperujo; Animal plasma; Animal tissues; Anti-oxidant; HPLC; Hydroxytyrosol; Olive oil; Tyrosol