title: Enhancement of anti-tumor immune responses by transfection of IFNγ gene into tumor using a novel type synthetic vector.
abstract: The existence of Th1 responses in a tumor microenvironment elicits a better prognosis for the patients. Transfection of Th1 polarizing cytokines, such as IFNγ, into tumor cells is an effective way to set up an appropriate microenvironment. Using a novel type synthetic vector composed of polyamidoamine dendrons, we transfected canine IFNγ gene into canine tumor cell lines, and examined direct and indirect effects of dendritic cells (DCs) against tumor growth in vitro. A cloned canine IFNγ gene expressed functional protein that induces maturation of DCs. When the canine IFNγ gene was transfected into canine tumor cell lines using the synthetic vector, most cells secreted canine IFNγ. Secretion of IFNγ reduced with time, but was maintained for 48h. DCs incubated with the IFNγ-transfected tumor cells exhibited greater suppressive activity and induced significantly higher cytotoxic activity against the tumor cells, relative to those incubated with untransfected tumor cells and comparable dose of IFNγ. Successful transfection of IFNγ by the synthetic vector efficiently enhanced the anti-tumor immune function of DCs, and sets up a suitable microenvironment for improvement in tumor therapy.
authors.affiliation: Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
authors.forename: Daluthgamage Patsy H
authors.initials: DP
authors.lastname: Wijesekera
authors.affiliation: Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan. Electronic address: sugiura@vet.osakafu-u.ac.jp.
authors.forename: Kikuya
authors.initials: K
authors.lastname: Sugiura
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, Sakai, Osaka, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Yuba
authors.affiliation: Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
authors.forename: Kana
authors.initials: K
authors.lastname: Ueda
authors.affiliation: Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
authors.forename: Viskam
authors.initials: V
authors.lastname: Wijewardana
authors.affiliation: Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
authors.forename: Ryouji
authors.initials: R
authors.lastname: Kanegi
authors.affiliation: Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
authors.forename: Toshiya
authors.initials: T
authors.lastname: Nishimura
authors.affiliation: Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ushigusa
authors.affiliation: Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Hatoya
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, Sakai, Osaka, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kono
authors.affiliation: Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Inaba
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D048708:Cell Growth Processes / Q000276:immunology; D045744:Cell Line, Tumor; D003601:Cytotoxicity Tests, Immunologic / Q000662:veterinary; D003713:Dendritic Cells / Q000276:immunology; D004283:Dog Diseases / Q000276:immunology* / Q000473:pathology; D004285:Dogs; D005260:Female; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics; D007371:Interferon-gamma / Q000008:administration & dosage* / Q000235:genetics* / Q000276:immunology; D012516:Osteosarcoma / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D013964:Thyroid Neoplasms / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D014162:Transfection / Q000662:veterinary
chemical_list: D007371:Interferon-gamma
keywords: Anti-tumor effect; Dendritic cells; Gene transfection; IFNγ; Synthetic vector