title: Hotspot activating PRKD1 somatic mutations in polymorphous low-grade adenocarcinomas of the salivary glands.
abstract: Polymorphous low-grade adenocarcinoma (PLGA) is the second most frequent type of malignant tumor of the minor salivary glands. We identified PRKD1 hotspot mutations encoding p.Glu710Asp in 72.9% of PLGAs but not in other salivary gland tumors. Functional studies demonstrated that this kinase-activating alteration likely constitutes a driver of PLGA.
authors.affiliation: Department of Pathology, University Health Network, Toronto, Ontario, Canada.
authors.forename: Ilan
authors.initials: I
authors.lastname: Weinreb
authors.affiliation: Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
authors.forename: Salvatore
authors.identifier: 0000000326862939
authors.initials: S
authors.lastname: Piscuoglio
authors.affiliation: Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
authors.forename: Luciano G
authors.initials: LG
authors.lastname: Martelotto
authors.affiliation: 1] Informatics and Bio-Computing Program, Ontario Institute for Cancer Research, Toronto, Ontario, Canada. [2] Ontario Cancer Institute, Princess Margaret Cancer Centre, University Health Network, Toronto, Onatrio, Canada. [3] Campbell Family Institute for Cancer Research, Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario, Canada.
authors.forename: Daryl
authors.initials: D
authors.lastname: Waggott
authors.affiliation: Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
authors.forename: Charlotte K Y
authors.initials: CK
authors.lastname: Ng
authors.affiliation: Department of Pathology, University Health Network, Toronto, Ontario, Canada.
authors.forename: Bayardo
authors.initials: B
authors.lastname: Perez-Ordonez
authors.affiliation: Informatics and Bio-Computing Program, Ontario Institute for Cancer Research, Toronto, Ontario, Canada.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Harding
authors.affiliation: 1] Informatics and Bio-Computing Program, Ontario Institute for Cancer Research, Toronto, Ontario, Canada. [2] Ontario Cancer Institute, Princess Margaret Cancer Centre, University Health Network, Toronto, Onatrio, Canada. [3] Campbell Family Institute for Cancer Research, Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario, Canada. [4] Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Javier
authors.initials: J
authors.lastname: Alfaro
authors.affiliation: Informatics and Bio-Computing Program, Ontario Institute for Cancer Research, Toronto, Ontario, Canada.
authors.forename: Kenneth C
authors.initials: KC
authors.lastname: Chu
authors.affiliation: Integrated Genomics Operation, Center for Molecular Oncology, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
authors.forename: Agnes
authors.initials: A
authors.lastname: Viale
authors.affiliation: 1] Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA. [2] School of Pathology, University of Milan, Milan, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Fusco
authors.affiliation: 1] Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA. [2] Instituto Português de Oncologia, Oporto, Portugal.
authors.forename: Arnaud
authors.initials: A
authors.lastname: da Cruz Paula
authors.affiliation: Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
authors.forename: Caterina
authors.initials: C
authors.lastname: Marchio
authors.affiliation: Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
authors.forename: Rita A
authors.initials: RA
authors.lastname: Sakr
authors.affiliation: Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
authors.forename: Raymond
authors.initials: R
authors.lastname: Lim
authors.affiliation: Department of Pathology, Kaiser Permanente, Woodland Hills Medical Center, Woodland Hills, California, USA.
authors.forename: Lester D R
authors.identifier: 0000000337141432
authors.initials: LD
authors.lastname: Thompson
authors.affiliation: Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA.
authors.forename: Simion I
authors.initials: SI
authors.lastname: Chiosea
authors.affiliation: Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA.
authors.forename: Raja R
authors.initials: RR
authors.lastname: Seethala
authors.affiliation: Department of Pathology and Laboratory Medicine, Charles University in Prague, Plzen, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Skalova
authors.affiliation: Department of Pathology, University of Virginia Medical Center, Charlottesville, Virginia, USA.
authors.forename: Edward B
authors.initials: EB
authors.lastname: Stelow
authors.affiliation: 1] Instituto Português de Oncologia Francisco Gentil, Lisbon, Portugal. [2] Faculdade de Medicina de Lisboa, Lisbon, Portugal.
authors.forename: Isabel
authors.initials: I
authors.lastname: Fonseca
authors.affiliation: Department of Pathology, Virginia Mason Hospital and Seattle Medical Center, Seattle, Washington, USA.
authors.forename: Adel
authors.initials: A
authors.lastname: Assaad
authors.affiliation: 1] Ontario Cancer Institute, Princess Margaret Cancer Centre, University Health Network, Toronto, Onatrio, Canada. [2] Campbell Family Institute for Cancer Research, Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario, Canada.
authors.forename: Christine
authors.initials: C
authors.lastname: How
authors.affiliation: Informatics and Bio-Computing Program, Ontario Institute for Cancer Research, Toronto, Ontario, Canada.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Informatics and Bio-Computing Program, Ontario Institute for Cancer Research, Toronto, Ontario, Canada.
authors.forename: Richard
authors.identifier: 0000000281816348
authors.initials: R
authors.lastname: de Borja
authors.affiliation: Informatics and Bio-Computing Program, Ontario Institute for Cancer Research, Toronto, Ontario, Canada.
authors.forename: Michelle
authors.initials: M
authors.lastname: Chan-Seng-Yue
authors.affiliation: Western University, London, Ontario, Canada.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Howlett
authors.affiliation: Western University, London, Ontario, Canada.
authors.forename: Anthony C
authors.initials: AC
authors.lastname: Nichols
authors.affiliation: Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
authors.forename: Y Hannah
authors.initials: YH
authors.lastname: Wen
authors.affiliation: Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
authors.forename: Nora
authors.initials: N
authors.lastname: Katabi
authors.affiliation: Cancer Genomics Platform, Ontario Institute for Cancer Research, Toronto, Ontario, Canada.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Buchner
authors.affiliation: Cancer Genomics Platform, Ontario Institute for Cancer Research, Toronto, Ontario, Canada.
authors.forename: Laura
authors.initials: L
authors.lastname: Mullen
authors.affiliation: 1] Ontario Cancer Institute, Princess Margaret Cancer Centre, University Health Network, Toronto, Onatrio, Canada. [2] Campbell Family Institute for Cancer Research, Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario, Canada. [3] Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kislinger
authors.affiliation: 1] Ontario Cancer Institute, Princess Margaret Cancer Centre, University Health Network, Toronto, Onatrio, Canada. [2] Campbell Family Institute for Cancer Research, Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario, Canada. [3] Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Bradly G
authors.identifier: 000000028187592X
authors.initials: BG
authors.lastname: Wouters
authors.affiliation: 1] Ontario Cancer Institute, Princess Margaret Cancer Centre, University Health Network, Toronto, Onatrio, Canada. [2] Campbell Family Institute for Cancer Research, Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario, Canada. [3] Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada. [4] Department of Radiation Oncology, Princess Margaret Hospital and University of Toronto, Toronto, Ontario, Canada.
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Liu
authors.affiliation: Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
authors.forename: Larry
authors.initials: L
authors.lastname: Norton
authors.affiliation: 1] Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada. [2] Department of Pathology, Virginia Mason Hospital and Seattle Medical Center, Seattle, Washington, USA.
authors.forename: John D
authors.initials: JD
authors.lastname: McPherson
authors.affiliation: 1] Department of Molecular Genetics, Lerner Research Institute, Cleveland, Ohio, USA. [2] Robert J. Tomsich Pathology and Laboratory Medicine Institute, Taussig Cancer Center, Cleveland Clinic, Cleveland, Ohio, USA.
authors.forename: Brian P
authors.initials: BP
authors.lastname: Rubin
authors.affiliation: Department of Pathology, University Health Network, Toronto, Ontario, Canada.
authors.forename: Blaise A
authors.initials: BA
authors.lastname: Clarke
authors.affiliation: Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
authors.forename: Britta
authors.initials: B
authors.lastname: Weigelt
authors.affiliation: 1] Informatics and Bio-Computing Program, Ontario Institute for Cancer Research, Toronto, Ontario, Canada. [2] Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada. [3] Department of Pharmacology and Toxicology, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Paul C
authors.identifier: 0000000305537520
authors.initials: PC
authors.lastname: Boutros
authors.affiliation: 1] Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA. [2].
authors.forename: Jorge S
authors.initials: JS
authors.lastname: Reis-Filho
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D051379:Mice; D018613:Microscopy, Confocal; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016296:Mutagenesis; D020125:Mutation, Missense / Q000235:genetics; D041681:NIH 3T3 Cells; D011493:Protein Kinase C / Q000737:chemistry / Q000235:genetics*; D012468:Salivary Gland Neoplasms / Q000235:genetics* / Q000473:pathology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: C089212:protein kinase D; D011493:Protein Kinase C