title: Multichamber Multipotentiostat System for Cellular Microphysiometry.
abstract: Multianalyte microphysiometry is a powerful technique for studying cellular metabolic flux in real time. Monitoring several analytes concurrently in a number of individual chambers, however, requires specific instrumentation that is not available commercially in a single, compact, benchtop form at an affordable cost. We developed a multipotentiostat system capable of performing simultaneous amperometric and potentiometric measurements in up to eight individual chambers. The modular design and custom LabVIEW™ control software provide flexibility and allow for expansion and modification to suit different experimental conditions. Superior accuracy is achieved when operating the instrument in a standalone configuration; however, measurements performed in conjunction with a previously developed multianalyte microphysiometer have shown low levels of crosstalk as well. Calibrations and experiments with primary and immortalized cell cultures demonstrate the performance of the instrument and its capabilities.
authors.affiliation: Department of Earth, Atmospheric, and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts. 02139, USA ; Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt University, Nashville, Tennessee. 37235, USA.
authors.forename: Eduardo A
authors.initials: EA
authors.lastname: Lima
authors.affiliation: Department of Chemistry, Vanderbilt University, Nashville, Tennessee. 37235, USA.
authors.forename: Rachel M
authors.initials: RM
authors.lastname: Snider
authors.affiliation: Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt University, Nashville, Tennessee. 37235, USA ; Department of Physics and Astronomy, Vanderbilt University, Nashville, Tennessee. 37235, USA.
authors.forename: Ronald S
authors.initials: RS
authors.lastname: Reiserer
authors.affiliation: Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt University, Nashville, Tennessee. 37235, USA ; Department of Chemistry, Vanderbilt University, Nashville, Tennessee. 37235, USA.
authors.forename: Jennifer R
authors.initials: JR
authors.lastname: McKenzie
authors.affiliation: Department of Chemistry, Vanderbilt University, Nashville, Tennessee. 37235, USA.
authors.forename: Danielle W
authors.initials: DW
authors.lastname: Kimmel
authors.affiliation: Department of Chemistry, Vanderbilt University, Nashville, Tennessee. 37235, USA.
authors.forename: Sven E
authors.initials: SE
authors.lastname: Eklund
authors.affiliation: Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt University, Nashville, Tennessee. 37235, USA ; Department of Physics and Astronomy, Vanderbilt University, Nashville, Tennessee. 37235, USA ; Department of Biomedical Engineering, Vanderbilt University, Nashville, Tennessee. 37235, USA ; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee. 37235, USA.
authors.forename: John P
authors.initials: JP
authors.lastname: Wikswo
authors.affiliation: Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt University, Nashville, Tennessee. 37235, USA ; Department of Chemistry, Vanderbilt University, Nashville, Tennessee. 37235, USA.
authors.forename: David E
authors.initials: DE
authors.lastname: Cliffel
keywords: Keywords1: multipotentiostat; microphysiology; microphysiometry