title: Engineered UV-A light-responsive gene expression system for measuring sun cream efficacy in mammalian cell culture.
abstract: Light-dependent gene regulation systems are advantageous as they allow for precise spatio-temporal control of target gene expression. In this paper, we present a novel UV-A and blue-light-inducible gene control system that is based on the light-dependent heterodimerization of the CRY2 and C1BN domains. Upon their interaction, a transcription factor is released from the cell membrane and initiates target gene expression. Capitalizing on that, sun cream UV-A protection properties were measured intracellularly.
authors.affiliation: Department of Biosystems Science and Engineering, ETH Zürich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
authors.forename: Markus
authors.initials: M
authors.lastname: Wieland
authors.affiliation: Department of Biosystems Science and Engineering, ETH Zürich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
authors.forename: Marius
authors.initials: M
authors.lastname: Müller
authors.affiliation: Department of Biosystems Science and Engineering, ETH Zürich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
authors.forename: Andreas
authors.initials: A
authors.lastname: Kyburz
authors.affiliation: Department of Biosystems Science and Engineering, ETH Zürich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
authors.forename: Phillip
authors.initials: P
authors.lastname: Heissig
authors.affiliation: Department of Biosystems Science and Engineering, ETH Zürich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Wekenmann
authors.affiliation: Department of Biosystems Science and Engineering, ETH Zürich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
authors.forename: Franziska
authors.initials: F
authors.lastname: Stolz
authors.affiliation: Department of Biosystems Science and Engineering, ETH Zürich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
authors.forename: Simon
authors.initials: S
authors.lastname: Ausländer
authors.affiliation: Department of Biosystems Science and Engineering, ETH Zürich, Mattenstrasse 26, CH-4058 Basel, Switzerland; Faculty of Science, University of Basel, Mattenstrasse 26, CH-4058 Basel, Switzerland. Electronic address: fussenegger@bsse.ethz.ch.
authors.forename: Martin
authors.initials: M
authors.lastname: Fussenegger
mesh_terms: D000818:Animals; D015870:Gene Expression / Q000235:genetics / Q000528:radiation effects; D015202:Protein Engineering; D058615:Synthetic Biology; D014466:Ultraviolet Rays
keywords: Gene control; Optogenetic sun cream profiler; Protein engineering; Synthetic biology