title: Dynamic changes in genes related to glucose uptake and utilization during pig skeletal and cardiac muscle development.
abstract: Skeletal and cardiac muscle have important roles in glucose uptake and utilization. However, changes in expression of protein coding genes and miRNAs that participate in glucose metabolism during development are not fully understood. In this study, we investigated the expression of genes related to glucose metabolism during muscle development. We found an age-dependent increase in gene expression in cardiac muscle, with enrichment in heart development- and energy-related metabolic processes. A subset of genes that were up-regulated until 30 or 180 days postnatally, and then down-regulated in psoas major muscle was significantly enriched in mitochondrial oxidative-related processes, while genes that up-regulated in longissimus doris muscle was significantly enriched in glycolysis-related processes. Meanwhile, expression of energy-related microRNAs decreased with increasing age. In addition, we investigated the correlation between microRNAs and mRNAs in three muscle types across different stages of development and found many potential microRNA-mRNA pairs involved in regulating glucose metabolism.
authors.affiliation: a Institute of Animal Genetics and Breeding, College of Animal Science and Technology , Sichuan Agricultural University , Ya'an , P.R. China.
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Guo
authors.forename: Long
authors.initials: L
authors.lastname: Jin
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Wang
authors.forename: Mengnan
authors.initials: M
authors.lastname: He
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Mingzhou
authors.initials: M
authors.lastname: Li
authors.forename: Surong
authors.initials: S
authors.lastname: Shuai
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000235:genetics; D004734:Energy Metabolism / Q000235:genetics; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006321:Heart / Q000254:growth & development*; D035683:MicroRNAs / Q000235:genetics; D024510:Muscle Development / Q000235:genetics*; D018482:Muscle, Skeletal / Q000254:growth & development* / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013552:Swine; D059467:Transcriptome
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D005947:Glucose
keywords: development; expression pattern; glucose metabolism; muscle; pig