title: Local corticotropin releasing hormone (CRH) signals to its receptor CRHR1 during postnatal development of the mouse olfactory bulb.
abstract: Neuropeptides play important physiological functions during distinct behaviors such as arousal, learning, memory, and reproduction. However, the role of local, extrahypothalamic neuropeptide signaling in shaping synapse formation and neuronal plasticity in the brain is not well understood. Here, we characterize the spatiotemporal expression profile of the neuropeptide corticotropin-releasing hormone (CRH) and its receptor CRHR1 in the mouse OB throughout development. We found that CRH-expressing interneurons are present in the external plexiform layer, that its cognate receptor is expressed by granule cells, and show that both CRH and CRHR1 expression enriches in the postnatal period when olfaction becomes important towards olfactory-related behaviors. Further, we provide electrophysiological evidence that CRHR1-expressing granule cells functionally respond to CRH ligand, and that the physiological circuitry of CRHR1 knockout mice is abnormal, leading to impaired olfactory behaviors. Together, these data suggest a physiologically relevant role for local CRH signaling towards shaping the neuronal circuitry within the mouse OB.
authors.affiliation: Program in Developmental Biology, Baylor College of Medicine, Houston, TX, 77030, USA.|Medical Scientist Training Program, Baylor College of Medicine, Houston, TX, 77030, USA.
authors.forename: Isabella
authors.initials: I
authors.lastname: Garcia
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, 77030, USA.
authors.forename: Paramjit K
authors.initials: PK
authors.lastname: Bhullar
authors.affiliation: Program in Developmental Biology, Baylor College of Medicine, Houston, TX, 77030, USA.
authors.forename: Burak
authors.initials: B
authors.lastname: Tepe
authors.affiliation: Program in Developmental Biology, Baylor College of Medicine, Houston, TX, 77030, USA.
authors.forename: Joshua
authors.initials: J
authors.lastname: Ortiz-Guzman
authors.affiliation: Department of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
authors.forename: Longwen
authors.initials: L
authors.lastname: Huang
authors.affiliation: Program in Developmental Biology, Baylor College of Medicine, Houston, TX, 77030, USA.
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Herman
authors.affiliation: Program in Developmental Biology, Baylor College of Medicine, Houston, TX, 77030, USA.
authors.forename: Lesley
authors.initials: L
authors.lastname: Chaboub
authors.affiliation: Program in Developmental Biology, Baylor College of Medicine, Houston, TX, 77030, USA.|Department of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.|The Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Houston, TX, 77030, USA.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Deneen
authors.affiliation: Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, TX, 77030, USA.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Justice
authors.affiliation: Program in Developmental Biology, Baylor College of Medicine, Houston, TX, 77030, USA. arenkiel@bcm.edu.|Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, 77030, USA. arenkiel@bcm.edu.|Department of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA. arenkiel@bcm.edu.|The Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Houston, TX, 77030, USA. arenkiel@bcm.edu.
authors.forename: Benjamin R
authors.initials: BR
authors.lastname: Arenkiel
mesh_terms: D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D004192:Discrimination, Psychological / Q000502:physiology; D019706:Excitatory Postsynaptic Potentials; D005260:Female; D007395:Interneurons / Q000378:metabolism / Q000502:physiology*; D008570:Memory, Short-Term / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D009812:Odorants; D009830:Olfactory Bulb / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D055696:Olfactory Perception / Q000502:physiology*; D018019:Receptors, Corticotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism*; D000099052:CRF Receptor, Type 1
chemical_list: D018019:Receptors, Corticotropin-Releasing Hormone; D000099052:CRF Receptor, Type 1; D003346:Corticotropin-Releasing Hormone
keywords: CRF; CRFR1; CRH; CRHR1; EPL; GPCR; Granule cell; Neuropeptide; Olfactory