title: Adenoviral-mediated GDNF protects bone marrow mesenchymal stem cells against apoptosis induced by hydrogen peroxide.
abstract: In this study, the effects of adenoviral-mediated glial cell line-derived neurotrophic factor (Ad-GDNF) on apoptosis of cultured bone marrow mesenchymal stem cells (BMSCs) induced by hydrogen peroxide (H2O2) were investigated. After BMSCs infected with Ad-GDNF were treated with 500 μM H2O2 at 37° C for 1 h, lactate dehydrogenase (LDH), MTT and TUNEL methods were used to detect cell viability and apoptosis. In addition, the levels of GDNF in the supernatants in BMSCs cultures were detected by ELISA, pro-inflammatory cytokines IL-6 and TNF-a in the supernatants and apoptosis-related protein Bax and Bcl-2 in cell pellets were investigated. The results showed that H2O2 treatment significantly induced apoptosis of BMSCs and decreased the viability of BMSCs. However, Ad-GDNF significantly reversed the effects of H2O2 on BMSCs. Furthermore, Ad-GDNF significantly decreased the levels of IL-6 and TNF-a and increased Bcl-2/Bax ratio in BMSCs treated by H2O2. In conclusion, Ad-GDNF inhibits apoptosis of BMSCs induced by H2O2, and the mechanisms may be related to down-regulating the expression of IL-6 and TNF-a and up-regulating the ratio of Bcl-2/Bax in BMSCs.
authors.affiliation: Department of Anatomy and Neurobiology, Luzhou Medical College, Sichuan Luzhou 646000, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Anatomy and Neurobiology, Luzhou Medical College, Sichuan Luzhou 646000, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Du
authors.affiliation: Department of Anatomy and Neurobiology, Luzhou Medical College, Sichuan Luzhou 646000, China.
authors.forename: Guangbi
authors.initials: G
authors.lastname: Fan
authors.affiliation: Department of Anatomy and Neurobiology, Luzhou Medical College, Sichuan Luzhou 646000, China.
authors.forename: Chaoxian
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Anatomy and Neurobiology, Luzhou Medical College, Sichuan Luzhou 646000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Deng
authors.affiliation: Department of Anatomy and Neurobiology, Tongji University School of Medicine, Shanghai 200092, China.
authors.forename: Qionglan
authors.initials: Q
authors.lastname: Yuan
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D005822:Genetic Vectors / Q000235:genetics; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000235:genetics / Q000276:immunology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D014162:Transfection / Q000379:methods*
chemical_list: D051100:Glial Cell Line-Derived Neurotrophic Factor; D006861:Hydrogen Peroxide
keywords: Glial cell line-derived neurotrophic factor; apoptosis; bone marrow mesenchymal stem cells; hydrogen peroxide