title: Base-cleavable microarrays for the characterization of DNA and RNA oligonucleotides synthesized in situ by photolithography.
abstract: Assessing synthesis efficiency, errors, failed deprotections, and chemical and enzymatic degradation of oligonucleotides on microarrays is essential for improving existing in situ synthesis methods, and for the development of new chemistries. We describe the use of LC-MS to analyse DNA and RNA oligonucleotides deprotected and cleaved under basic conditions from microarrays fabricated using light-directed in situ chemistry. The data yield essential information on array quality and sequence identity.
authors.affiliation: Department of Chemistry, McGill University, Montréal, Québec H3A 0B8, Canada. masad.damha@mcgill.ca.
authors.forename: Jory
authors.initials: J
authors.lastname: Lietard
authors.forename: Nicole
authors.initials: N
authors.lastname: Kretschy
authors.forename: Matej
authors.initials: M
authors.lastname: Sack
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Wahba
authors.forename: Mark M
authors.initials: MM
authors.lastname: Somoza
authors.forename: Masad J
authors.initials: MJ
authors.lastname: Damha
mesh_terms: D002853:Chromatography, Liquid; D004247:DNA / Q000737:chemistry*; D013058:Mass Spectrometry; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D012313:RNA; D004247:DNA