title: Study of MDM2 and SUMO-1 expression in actinic cheilitis and lip cancer.
abstract: Actinic cheilitis exhibits a potential of malignant transformation in 10-20 % of cases. The objective of this study was to compare the expression of MDM2 and SUMO-1 proteins between actinic cheilitis (AC) and squamous cell carcinoma (SCC) of the lip. The sample consisted of lower lip mucosa specimens obtained from cases with a clinical and histopathological diagnosis of AC (n = 26) and SCC (n = 25) and specimens of labial semi-mucosa (n = 15) without clinical alterations or inflammation. The tissue samples were stained with hematoxylin-eosin and anti-MDM2 and anti-SUMO-1 antibodies. Data were analyzed by the Kruskal-Wallis and Dunn's tests (5 %). The median expression of MDM2 (kW = 36.8565; df = 3-1 = 2; p = 0.0001) and SUMO-1 (kW = 32.7080; df = 3-1 = 2; p = 0.0001) was similar in cases of AC and SCC of the lip, but differed significantly from that observed for normal labial semi-mucosa. Despite the limitations of the present study, immunohistochemistry demonstrated the overexpression of important proteins (MDM2 and SUMO-1) related to regulatory mechanisms of apoptosis in AC and SCC of the lip, but further studies are needed.
authors.affiliation: Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, UNESP, Univ Estadual Paulista, 777 Engenheiro Francisco José Longo Avenue, São Dimas, São José Dos Campos, SP, 12245-000, Brazil, mgoliveiraalves@gmail.com.
authors.forename: Mônica Ghislaine
authors.initials: MG
authors.lastname: Oliveira Alves
authors.forename: Adriana
authors.initials: A
authors.lastname: da Mota Delgado
authors.forename: Ivan
authors.initials: I
authors.lastname: Balducci
authors.forename: Yasmin Rodarte
authors.initials: YR
authors.lastname: Carvalho
authors.forename: Ana Sueli Rodrigues
authors.initials: AS
authors.lastname: Cavalcante
authors.forename: Janete Dias
authors.initials: JD
authors.lastname: Almeida
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology; D016022:Case-Control Studies; D002613:Cheilitis / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008048:Lip Neoplasms / Q000201:enzymology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000201:enzymology* / Q000473:pathology; D051736:Proto-Oncogene Proteins c-mdm2 / Q000032:analysis*; D025842:SUMO-1 Protein / Q000032:analysis*; D015854:Up-Regulation
chemical_list: D025842:SUMO-1 Protein; C495501:SUMO1 protein, human; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2