title: Spectroscopic analyses on interaction of bovine serum albumin with novel spiro[cyclopropane-pyrrolizin].
abstract: The interaction between novel spiro[cyclopropane-pyrrolizin] (NSCP) and bovine serum albumin (BSA) was analyzed by fluorescence and ultraviolet-visible (UV-Vis) spectroscopy at 298 K, 304 K and 310 K under simulative physiological conditions. The results showed that NSCP can effectively quench the intrinsic fluorescence of BSA via static quenching. The binding constants, binding sites of NSCP with BSA were calculated. Hydrogen binds and van der Waals force played a major role in stabilizing the complex and the binding reaction were spontaneous. According to the Förster non-radiation energy transfer theory, the average binding distances between NSCP and BSA were obtained. What is more, the synchronous fluorescence spectra indicated that the conformation of BSA has been changed.
authors.affiliation: Key Laboratory of Theoretical Organic Chemistry and Function Molecule, Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China; State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen 361005, China; Key Laboratory of Computational Physical Sciences (Fudan University), Ministry of Education, Shanghai 200433, China. Electronic address: yu_xianyong@163.com.
authors.forename: Xianyong
authors.initials: X
authors.lastname: Yu
authors.affiliation: Key Laboratory of Theoretical Organic Chemistry and Function Molecule, Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China.
authors.forename: Zhixi
authors.initials: Z
authors.lastname: Liao
authors.affiliation: Key Laboratory of Theoretical Organic Chemistry and Function Molecule, Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China.
authors.forename: Bingfei
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Theoretical Organic Chemistry and Function Molecule, Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China.
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Hu
authors.affiliation: Key Laboratory of Theoretical Organic Chemistry and Function Molecule, Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China. Electronic address: lixiaofang@iccas.ac.cn.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D003521:Cyclopropanes / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011758:Pyrroles / Q000737:chemistry / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013141:Spiro Compounds / Q000737:chemistry / Q000378:metabolism*
chemical_list: D003521:Cyclopropanes; D011758:Pyrroles; D013141:Spiro Compounds; D012710:Serum Albumin, Bovine; C030797:cyclopropane
keywords: Bovine serum albumin; Fluorescence spectroscopy; Interaction; Novel spiro[cyclopropane-pyrrolizin]; Ultraviolet–visible spectroscopy