title: Isolation and characterization of 11 microsatellite markers for Glochidion acuminatum (Phyllanthaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed for Glochidion acuminatum (Phyllanthaceae) to investigate pollen dispersal distances and thereby to assess the effectiveness of specialized Epicephala moths as pollinators. •
  
  
  RESULTS
  Using next-generation sequencing, 11 polymorphic microsatellite primer pairs were developed for G. acuminatum. The primer pairs were tested on 49 individuals from two populations in Japan. The number of alleles per locus ranged from two to 13, and the expected heterozygosity ranged from 0.12 to 0.85. Probability of identity for all loci combined was lower than 1.27 × 10(7). •
  
  
  CONCLUSIONS
  The microsatellite markers developed in this study will be useful for evaluating the benefit of specialized Epicephala moth pollination to Glochidion plants.
authors.affiliation: Center for Ecological Research, Kyoto University, 2-509-3 Hirano, Otsu, Shiga 520-2113, Japan.
authors.forename: Ko
authors.initials: K
authors.lastname: Mochizuki
authors.affiliation: Center for Ecological Research, Kyoto University, 2-509-3 Hirano, Otsu, Shiga 520-2113, Japan ; Japan Science and Technology Agency, Precursory Research for Embryonic Science and Technology (PRESTO), Honcho 4-1-8, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Atsushi J
authors.initials: AJ
authors.lastname: Nagano
authors.affiliation: Center for Ecological Research, Kyoto University, 2-509-3 Hirano, Otsu, Shiga 520-2113, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kudoh
authors.affiliation: Center for Ecological Research, Kyoto University, 2-509-3 Hirano, Otsu, Shiga 520-2113, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kawakita
keywords: Epicephala; Glochidion acuminatum; Phyllantheae; active pollination; obligate pollination mutualism; pollen dispersal distance