title: Spatholobus suberectus inhibits osteoclastogenesis and stimulates chondrogenesis.
abstract: This study was carried out to investigate the effect of Spatholobus suberectus Dunn (SS) on the protection of chondral defect and inhibition of osteoclastogenesis. To examine these effects, we measured the matrix metalloproteinase (MMP) levels in SW1353 chondrosarcoma cells and performed tartrate-resistant acid phosphatase (TRAP) staining in bone marrow macrophage (BMM)-derived osteoclasts. To investigate the anti-osteoarthritis (OA) effects, we assessed TNF-α-induced MMP-1, -3, -9 and tissue inhibitors of matrix metalloproteinase (TIMP) expression levels in SW1353 cells. We observed that SS extract significantly inhibited MMP and TIMP expression in SW1353 cells. Also, SS extract inhibited the receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation. These results suggest that SS extract may have a potential in the treatment of bone loss and chondral defect by suppressing osteoclast differentiation and decreasing the expression of OA factors. Therefore, clarification of the mechanism of the action of SS extract and its active components is needed.
authors.affiliation: College of Pharmacy, Keimyung University, Dae-gu 704-701, Republic of Korea.
authors.forename: Nam-Kyung
authors.initials: NK
authors.lastname: Im
authors.forename: Sung-Gyu
authors.initials: SG
authors.lastname: Lee
authors.forename: Dong-Sung
authors.initials: DS
authors.lastname: Lee
authors.forename: Pil-Hoon
authors.initials: PH
authors.lastname: Park
authors.forename: In-Seon
authors.initials: IS
authors.lastname: Lee
authors.forename: Gil-Saeng
authors.initials: GS
authors.lastname: Jeong
mesh_terms: D000135:Acid Phosphatase; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D020219:Chondrogenesis / Q000187:drug effects*; D002813:Chondrosarcoma / Q000201:enzymology; D003864:Depression, Chemical; D007887:Fabaceae / Q000737:chemistry*; D007527:Isoenzymes; D008264:Macrophages; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D010003:Osteoarthritis / Q000188:drug therapy; D010010:Osteoclasts / Q000166:cytology* / Q000201:enzymology; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use*; D018517:Plant Roots; D053245:RANK Ligand; D013268:Stimulation, Chemical; D000071681:Tartrate-Resistant Acid Phosphatase; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D007527:Isoenzymes; D010936:Plant Extracts; D053245:RANK Ligand; D019714:Tissue Inhibitor of Metalloproteinases; D000135:Acid Phosphatase; C000605511:Acp5 protein, mouse; D000071681:Tartrate-Resistant Acid Phosphatase; D020782:Matrix Metalloproteinases
keywords: Matrix Metalloproteinases (MMPs); Osteoarthritis; Osteoclast; Osteoporosis; Spatholobus suberectus