title: Characterization of human small heat shock protein HspB1 that carries C-terminal domain mutations associated with hereditary motor neuron diseases.
abstract: Physico-chemical properties of four mutants (T164A, T180I, P182S and R188W) of human small heat shock protein HspB1 (Hsp27) associated with neurodegenerative diseases were analyzed by means of fluorescence spectroscopy, dynamic light scattering, size-exclusion chromatography and measurement of chaperone-like activity. Mutation T164A was accompanied by destabilization of the quaternary structure and decrease of thermal stability without any significant changes of chaperone-like activity. Mutations T180I and P182S are adjacent or within the conserved C-terminal motif IPI/V. Replacement T180⇒I leading to the formation of hydrophobic cluster consisting of three Ile produced small increase of thermal stability without changes of chaperone-like activity. Mutation P182S induced the formation of metastable large oligomers of HspB1 with apparent molecular weight of more than 1000kDa. Oligomers of P182S have very low thermal stability and undergo irreversible aggregation at low temperature. The P182S mutant forms mixed oligomers with the wild type HspB1 and the properties of these mixed oligomers are intermediate between those of the wild type HspB1 and its mutant. Mutation R188W did not significantly affect quaternary structure or thermal stability of HspB1, but was accompanied by a pronounced decrease of its chaperone-like activity. All mutations analyzed are associated with hereditary motor neuropathies or Charcot-Marie-Tooth disease type 2; however, molecular mechanisms underlying pathological effects are specific for each of these mutants.
authors.affiliation: Department of Biochemistry, School of Biology, Moscow State University, Moscow 119991, Russian Federation.
authors.forename: Anna S
authors.initials: AS
authors.lastname: Chalova
authors.affiliation: Department of Biochemistry, School of Biology, Moscow State University, Moscow 119991, Russian Federation.
authors.forename: Maria V
authors.initials: MV
authors.lastname: Sudnitsyna
authors.affiliation: Laboratory for Biocrystallography, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, Belgium.
authors.forename: Sergei V
authors.initials: SV
authors.lastname: Strelkov
authors.affiliation: Department of Biochemistry, School of Biology, Moscow State University, Moscow 119991, Russian Federation. Electronic address: NBGusev@mail.ru.
authors.forename: Nikolai B
authors.initials: NB
authors.lastname: Gusev
keywords: Chaperone-like activity; Hydrophobic properties; Oligomeric structure; Peripheral neuropathy; Small heat shock protein; Thermal stability