title: Increased polysaccharide production and biosynthetic gene expressions in a submerged culture of Ganoderma lucidum by the overexpression of the homologous α-phosphoglucomutase gene.
abstract: This study aimed to improve the production of polysaccharide by engineering the biosynthetic pathway in Ganoderma lucidum through the overexpression of α-phosphoglucomutase (PGM) gene. PGM is responsible for the linkage between sugar catabolism and sugar anabolism. The effects of PGM gene overexpression on intracellular polysaccharide (IPS) content, extracellular polysaccharide (EPS) production and transcription levels of three genes encoding the enzymes involved in polysaccharide biosynthesis, including PGM, UDP-glucose pyrophosphorylase (UGP), and β-1,3-glucan synthase (GLS), were investigated. The maximum IPS content and EPS production in G. lucidum overexpressing the PGM gene were 23.67 mg/100 mg dry weight and 1.76 g/L, respectively, which were higher by 40.5 and 44.3% than those of the wild-type strain. The transcription levels of PGM, UGP and GLS were upregulated by 4.77-, 1.51- and 1.53-fold, respectively, in the engineered strain, suggesting that increased polysaccharide biosynthesis may result from a higher expression of those genes.
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China, jwxu@kmust.edu.cn.
authors.forename: Jun-Wei
authors.initials: JW
authors.lastname: Xu
authors.forename: Sen-Lin
authors.initials: SL
authors.lastname: Ji
authors.forename: Huan-Jun
authors.initials: HJ
authors.lastname: Li
authors.forename: Jiang-Sheng
authors.initials: JS
authors.lastname: Zhou
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Duan
authors.forename: Li-Zhi
authors.initials: LZ
authors.lastname: Dang
authors.forename: Ming-He
authors.initials: MH
authors.lastname: Mo
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D005813:Genes, Synthetic / Q000235:genetics*; D024861:Genetic Enhancement / Q000379:methods*; D059765:Homologous Recombination / Q000235:genetics; D010733:Phosphoglucomutase / Q000502:physiology*; D011134:Polysaccharides / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D020940:Reishi / Q000502:physiology*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D011134:Polysaccharides; D010733:Phosphoglucomutase