title: Effects of Galla chinensis extracts on UVB-irradiated MMP-1 production in hairless mice.
abstract: Galla chinensis (GAC) is a natural traditional Chinese medicine that has been widely used in folk medicine. Although GAC compounds (mainly gallic acid and methyl gallate) possess strong antiviral, antibacterial, anticancer, and antioxidant activities, there is no report regarding topical or oral administration of GAC compounds on UVB irradiation-induced photoaging in hairless mice (SKH: HR-1). In the present study, we examined cell viability, intracellular reactive oxygen species (ROS), matrix metalloproteinase-1 (MMP-1), and interleukin-6 (IL-6) in skin fibroblasts and keratinocytes induced by UVB in vitro. We also studied skin damage by measuring skin thickness, elasticity, wrinkling and levels of protein MMP-1, elastin, procollagen type I, and transforming growth factor-β1 (TGF-β1) in hairless mouse skin chronically irradiated by UVB in vivo. GAC treatment significantly prevented skin photoaging by reducing the levels of ROS, MMP-1, and IL-6 and promoting production of elastin, procollagen type I, and TGF-β1. According to the results of H&E staining and Masson's trichrome staining, GAC reduced skin thickness and wrinkle formation while it increased skin elasticity. The effects of GAC on UVB-induced skin photoaging may be due to suppressed MMP-1 expression. These findings could be referenced for the development of new agents that target UVB-induced photoaging.
authors.affiliation: Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggido, 446-701, Republic of Korea.
authors.forename: Zheng-wang
authors.initials: ZW
authors.lastname: Sun
authors.forename: Eunson
authors.initials: E
authors.lastname: Hwang
authors.forename: Hyun Ji
authors.initials: HJ
authors.lastname: Lee
authors.forename: Tae Youp
authors.initials: TY
authors.lastname: Lee
authors.forename: Hyun Geun
authors.initials: HG
authors.lastname: Song
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Park
authors.forename: Heon-Sub
authors.initials: HS
authors.lastname: Shin
authors.forename: Don-Gil
authors.initials: DG
authors.lastname: Lee
authors.forename: Tae Hoo
authors.initials: TH
authors.lastname: Yi
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis*; D008812:Mice, Hairless; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012867:Skin / Q000033:anatomy & histology / Q000187:drug effects / Q000528:radiation effects; D015595:Skin Aging / Q000187:drug effects* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D024042:Collagen Type I; D004365:Drugs, Chinese Herbal; D015850:Interleukin-6; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D020781:Matrix Metalloproteinase 1