title: Molecular requirement for sterols in herpes simplex virus entry and infectivity.
abstract: Herpes simplex virus 1 (HSV-1) required cholesterol or desmosterol for virion-induced membrane fusion. HSV successfully entered DHCR24(-/-) cells, which lack a desmosterol-to-cholesterol conversion enzyme, indicating that entry can occur independently of cholesterol. Depletion of desmosterol from these cells resulted in diminished HSV-1 entry, suggesting a general sterol requirement for HSV-1 entry and that desmosterol can operate in virus entry. Cholesterol functioned more effectively than desmosterol, suggesting that the hydrocarbon tail of cholesterol influences viral entry.
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington, USA.
authors.forename: George A
authors.initials: GA
authors.lastname: Wudiri
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA.
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Pritchard
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA Animal Disease Research Unit, USDA Agricultural Research Service, Washington State University, Pullman, Washington, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington, USA.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.affiliation: Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington, USA.
authors.forename: Hector C
authors.initials: HC
authors.lastname: Aguilar
authors.affiliation: Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, USA.
authors.forename: Stacey D
authors.initials: SD
authors.lastname: Gilk
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA nicola@vetmed.wsu.edu.
authors.forename: Anthony V
authors.initials: AV
authors.lastname: Nicola
mesh_terms: D000818:Animals; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D003897:Desmosterol / Q000378:metabolism*; D018259:Herpesvirus 1, Human / Q000502:physiology*; D053586:Virus Internalization
chemical_list: D003897:Desmosterol; D002784:Cholesterol