title: Chitosan-alginate membranes accelerate wound healing.
abstract: The purpose of this study was to evaluate the efficacy of chitosan-alginate membrane to accelerate wound healing in experimental cutaneous wounds. Two wounds were performed in Wistar rats by punching (1.5 cm diameter), treated with membranes moistened with saline solution (CAM group) or with saline only (SL group). After 2, 7, 14, and 21 days of surgery, five rats of each group were euthanized and reepithelialization was evaluated. The wounds/scars were harvested for histological, flow cytometry, neutrophil infiltrate, and hydroxyproline analysis. CAM group presented higher inflammatory cells recruitment as compared to SL group on 2(nd) day. On the 7(th) day, CAM group showed higher CD11b(+) level and lower of neutrophils than SL group. The CAM group presented higher CD4(+) cells influx than SL group on 2(nd) day, but it decreased during the follow up and became lower on 14(th) and 21(st) days. Higher fibroplasia was noticed on days 7 and 14 as well as higher collagenesis on 21(st) in the CAM group in comparison to SL group. CAM group showed faster reepithelialization on 7(th) day than SL group, although similar in other days. In conclusion, chitosan-alginate membrane modulated the inflammatory phase, stimulated fibroplasia and collagenesis, accelerating wound healing process in rats.
authors.affiliation: Biotecnology Post Graduate Program, Institute of Biomedical Sciences, University of São Paulo (USP), São Paulo, São Paulo, Brazil.
authors.forename: Guilherme Ferreira
authors.initials: GF
authors.lastname: Caetano
authors.affiliation: Department of Internal Medicine, Division of Dermatology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.
authors.forename: Marco Andrey Cipriani
authors.initials: MA
authors.lastname: Frade
authors.affiliation: Department of Internal Medicine, Division of Dermatology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.
authors.forename: Thiago Antônio Moretti
authors.initials: TA
authors.lastname: Andrade
authors.affiliation: Department of Internal Medicine, Division of Dermatology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.
authors.forename: Marcel Nani
authors.initials: MN
authors.lastname: Leite
authors.affiliation: Department of Engineering of Materials and of Bioprocesses, School of Chemical Engineering, State University of Campinas, Campinas, São Paulo, Brazil.
authors.forename: Cecilia Zorzi
authors.initials: CZ
authors.lastname: Bueno
authors.affiliation: Department of Engineering of Materials and of Bioprocesses, School of Chemical Engineering, State University of Campinas, Campinas, São Paulo, Brazil.
authors.forename: Ângela Maria
authors.initials: ÂM
authors.lastname: Moraes
authors.affiliation: Department of Biology, São Paulo State University (UNESP), Assis, São Paulo, Brazil.
authors.forename: João Tadeu
authors.initials: JT
authors.lastname: Ribeiro-Paes
mesh_terms: D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D048271:Chitosan / Q000494:pharmacology*; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D008567:Membranes, Artificial; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000378:metabolism / Q000473:pathology / Q000628:therapy
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D008567:Membranes, Artificial; D020723:Glucuronic Acid; D048271:Chitosan; D006909:Hydroxyproline
keywords: alginate; chitosan; collagen; membrane; wound healing