title: Comparison of plasma microRNA-1 and cardiac troponin T in early diagnosis of patients with acute myocardial infarction.
abstract: BACKGROUND
  Early reperfusion can effectively treat acute myocardial infarction (AMI) and reduce the mortality significantly. This study aimed to compare the role of plasma microRNA-1 (miR-1) and cardiac troponin T (cTnT) in early diagnosis of AMI patients.
  
  
  METHODS
  From May 2011 to May 2012, plasma samples were collected from 56 AMI patients and 28 non-AMI controls. The expression of plasma miR-1 was measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and the level of plasma cTnT was measured using electrochemiluminescence-based methods on an Elecsys 2010 Immunoassay Analyzer. SPSS 16.0 was used for the statistical analysis of the results. Data were expressed as mean±standard deviation unless otherwise described. The differences about clinical characteristics between the AMI patients and controls were tested using Student's t test or Fisher's exact test. The Mann-Whitney U test was conducted to compare the expression of microRNAs between the AMI patients and controls. MicroRNAs expression between different intervals of the AMI patients was compared using Wilcoxon's signed-rank test. The receiver operating characteristic (ROC) curve was established to discriminate the AMI patients from the controls.
  
  
  RESULTS
  In the present study, the expression of plasma miR-1 was significantly increased in the AMI patients compared with the healthy controls (P<0.01). The plasma miR-1 in the AMI patients decreased to the normal level at 14 days (P>0.05). The expression of plasma miR-1 was not related to the clinical characteristics of the study population (P>0.05). ROC curve analyses demonstrated that miR-1 was specific and sensitive for the early diagnosis of AMI, but not superior to cTnT.
  
  
  CONCLUSIONS
  Plasma miR-1 could be used in the early diagnosis of AMI, but it is similar to cTnT.
authors.affiliation: Department of Emergency Medicine, Pengpai Memorial Hospital of Haifeng, Haifeng, China.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Li
authors.affiliation: Department of Emergency Medicine, Pengpai Memorial Hospital of Haifeng, Haifeng, China.
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Cai
authors.affiliation: Department of Emergency Medicine, Pengpai Memorial Hospital of Haifeng, Haifeng, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Ye
authors.affiliation: Department of Emergency Medicine, Pengpai Memorial Hospital of Haifeng, Haifeng, China.
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Liu
authors.affiliation: Department of Emergency Medicine, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Emergency Medicine, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Xiao-Xing
authors.initials: XX
authors.lastname: Liao
keywords: Acute myocardial infarction; Biomarker; Early diagnosis; High sensitive cardiac troponin T; MicroRNA-1; Sensitivity; Specificity