title: Stable SET knockdown in breast cell carcinoma inhibits cell migration and invasion.
abstract: Breast cancer is the most malignant tumor for women, however, the mechanisms underlying this devastating disease remain unclear. SET is an endogenous inhibitor of protein phosphatase 2A (PP2A) and involved in many physiological and pathological processes. SET could promote the occurrence of tumor through inhibiting PP2A. In this study, we explore the role of SET in the migration and invasion of breast cancer cells MDA-MB-231 and ZR-75-30. The stable suppression of SET expression through lentivirus-mediated RNA interference (RNAi) was shown to inhibit the growth, migration and invasion of breast cancer cells. Knockdown of SET increases the activity and expression of PP2Ac and decrease the expression of matrix metalloproteinase 9 (MMP-9). These data demonstrate that SET may be involved in the pathogenic processes of breast cancer, indicating that SET can serve as a potential therapeutic target for the treatment of breast cancer.
authors.affiliation: Department of Occupational Health and Occupational Medicine, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China; Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Xi-fei
authors.initials: XF
authors.lastname: Yang
authors.affiliation: Department of Occupational Health and Occupational Medicine, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China; Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Xiao-hu
authors.initials: XH
authors.lastname: Ren
authors.affiliation: Department of Occupational Health and Occupational Medicine, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China.
authors.forename: Xiao-jing
authors.initials: XJ
authors.lastname: Meng
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Huang
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, China.
authors.forename: Qiong-hui
authors.initials: QH
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Jian-hui
authors.initials: JH
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Wen-xu
authors.initials: WX
authors.lastname: Hong
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xia
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Xin-feng
authors.initials: XF
authors.lastname: Huang
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen, China. Electronic address: bio-research@hotmail.com.
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Liu
authors.affiliation: Department of Occupational Health and Occupational Medicine, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China. Electronic address: zoufei616@163.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Zou
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology* / Q000503:physiopathology* / Q000628:therapy; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D004268:DNA-Binding Proteins; D005260:Female; D055785:Gene Knockdown Techniques; D056488:Histone Chaperones / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000502:physiology; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000503:physiopathology / Q000517:prevention & control; D054648:Protein Phosphatase 2 / Q000037:antagonists & inhibitors / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D014157:Transcription Factors / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000502:physiology
chemical_list: D004268:DNA-Binding Proteins; D056488:Histone Chaperones; D034741:RNA, Small Interfering; C513473:SET protein, human; D014157:Transcription Factors; D054648:Protein Phosphatase 2; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse
keywords: Breast cancer; MMP-9; PP2Ac; RNAi; SET