title: Comparison of the detection rates of RT-PCR and virus culture using a combination of specimens from multiple sites for enterovirus-associated encephalomyelitis during enterovirus 71 epidemic.
abstract: Enterovirus (EV) 71 may cause severe neurological illness in the pediatric population. The present study aimed to compare the detection rates of reverse transcription-polymerase chain reaction (RT-PCR) with pan-EV/EV71 type- specific primers and virus culture (VC) for the identification of EV and EV71 using specimens from multiple sites. In total, specimens from throat/rectal swabs, cerebrospinal fluid (CSF), and blood from 66 patients diagnosed with EV encephalomyelitis were subjected to both RT-PCR and VC for detecting the presence of pan-EV and EV71. The results revealed that the positive RT-PCR rate was higher in throat swabs (60.6%) and rectum swabs (50.0%) than in CSF (16.7%) and blood (15.6%). The same trend was also observed in case of VC: throat swabs (22.7%), rectum swabs (10.6%), and blood (3.0%). The detection rate of EV encephalomyelitis by RT-PCR was 90.9% for all samples, 63.6% of which were subtyped as EV71. The detection rates of RT-PCR were superior to those of VC, and identification using specimens from throat/rectal swabs yielded higher positive results. These findings may help physicians to identify the etiologies at an early stage during EV71 epidemics and to make emergent medical decisions for minimizing patient morbidity and mortality.
authors.affiliation: Institute of Medicine, Chung Shan Medical University.
authors.forename: Jeng-Dau
authors.initials: JD
authors.lastname: Tsai
authors.forename: Henry J
authors.initials: HJ
authors.lastname: Tsai
authors.forename: Tsai-Hsiu
authors.initials: TH
authors.lastname: Lin
authors.forename: Yuan-Yen
authors.initials: YY
authors.lastname: Chang
authors.forename: Sheng-Hui
authors.initials: SH
authors.lastname: Yang
authors.forename: Hung-Tsung
authors.initials: HT
authors.lastname: Kuo
mesh_terms: D000818:Animals; D002675:Child, Preschool; D019411:Clinical Laboratory Techniques / Q000379:methods*; D018792:Encephalitis, Viral / Q000175:diagnosis* / Q000821:virology; D029821:Enterovirus A, Human / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000821:virology; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014776:Virus Cultivation / Q000379:methods