title: Swept-source OCT angiography of the retinal vasculature using intensity differentiation-based optical microangiography algorithms.
abstract: OBJECTIVE
  To demonstrate the feasibility of using a 1,050-nm swept-source optical coherence tomography (SS-OCT) system to achieve noninvasive retinal vasculature imaging in human eyes.
  
  
  METHODS
  Volumetric data sets were acquired using a 1-µm SS-OCT prototype that operated at a 100-kHz A-line rate. A scanning protocol designed to allow for motion contrast processing, referred to as OCT angiography or optical microangiography (OMAG), was used to scan an approximately 3 × 3–mm area in the central macular region of the retina within approximately 4.5 seconds. An intensity differentiation-based OMAG algorithm was used to extract three-dimensional retinal functional microvasculature information.
  
  
  RESULTS
  Intensity signal differentiation generated capillary-level resolution en face OMAG images of the retina. The parafoveal capillaries were clearly visible, thereby allowing visualization of the foveal avascular zone in healthy subjects.
  
  
  CONCLUSIONS
  The capability of OMAG to produce retinal vascular images was demonstrated using the 1-µm SS-OCT prototype. This technique has potential clinical value for studying retinal vasculature abnormalities.
authors.affiliation: Department of Bioengineering, University of Washington, Seattle, Washington, USA.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Huang
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Mariana R
authors.initials: MR
authors.lastname: Thorell
authors.forename: Lin
authors.initials: L
authors.lastname: An
authors.forename: Mary K
authors.initials: MK
authors.lastname: Durbin
authors.forename: Michal
authors.initials: M
authors.lastname: Laron
authors.forename: Utkarsh
authors.initials: U
authors.lastname: Sharma
authors.forename: Giovanni
authors.initials: G
authors.lastname: Gregori
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Rosenfeld
authors.forename: Ruikang K
authors.initials: RK
authors.lastname: Wang
mesh_terms: D000328:Adult; D000465:Algorithms; D002452:Cell Count; D005260:Female; D005451:Fluorescein Angiography / Q000379:methods*; D005654:Fundus Oculi; D064368:Healthy Volunteers; D006801:Humans; D021621:Imaging, Three-Dimensional; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012171:Retinal Vessels / Q000166:cytology*; D041623:Tomography, Optical Coherence / Q000379:methods*