title: Development of experimental in vitro burn model.
abstract: OBJECTIVE
  To propose an experimental burn model in NIH-3T3 cell line.
  
  
  METHODS
  Induction of thermal injury in cultures of mouse fibroblast - NIH-3T3- cell line and determination of cell viability by MTT and immunofluorescence.
  
  
  RESULTS
  The heating of the Petri dish increased proportionally to the temperature of the base and the time of exposure to microwave. In this in vitro burn model, using the cell line NIH-3T3 was observed drastic cellular injury with significant changes in cell viability and activity. It showed drastically modified cell morphology with altered membrane, cytoskeleton and nucleus, and low cellularity compared to the control group.
  
  
  CONCLUSIONS
  The burn model in vitro using the cell line NIH-3T3 was reproductive and efficient. This burn model was possible to determine significant changes in cell activity and decreased viability, with drastic change in morphology, cell lysis and death.
authors.affiliation: Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Ana Carolina Morais
authors.initials: AC
authors.lastname: Fernandes
authors.affiliation: Department of Biological Sciences, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil.
authors.forename: Jerônimo Pereira de
authors.initials: JP
authors.lastname: França
authors.affiliation: Department of Biological Sciences, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil.
authors.forename: Silvana
authors.initials: S
authors.lastname: Gaiba
authors.affiliation: Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Antonio Carlos
authors.initials: AC
authors.lastname: Aloise
authors.affiliation: Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Andrea Fernandes de
authors.initials: AF
authors.lastname: Oliveira
authors.affiliation: Department of Biological Sciences, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil.
authors.forename: Andrea Aparecida de Fátima Souza
authors.initials: AA
authors.lastname: Moraes
authors.affiliation: Department of Biological Sciences, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil.
authors.forename: Lucimar Pereira de
authors.initials: LP
authors.lastname: França
authors.affiliation: UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Lydia Masako
authors.initials: LM
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D002056:Burns / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival; D004195:Disease Models, Animal; D005455:Fluorescent Antibody Technique; D005562:Formazans; D006358:Hot Temperature; D066298:In Vitro Techniques / Q000379:methods; D051379:Mice; D018613:Microscopy, Confocal; D041681:NIH 3T3 Cells; D015203:Reproducibility of Results; D013778:Tetrazolium Salts; D013997:Time Factors
chemical_list: D005562:Formazans; D013778:Tetrazolium Salts; C079782:MTT formazan