title: Characterization of femtosecond laser-induced breakdown spectroscopy (fsLIBS) and applications for biological samples.
abstract: We characterize the femtosecond laser-induced breakdown spectroscopy (fsLIBS) signal for biological tissues as a function of different excitation parameters with femtosecond laser systems. These parameters include laser energy, depth of focus, and number of pulses per focal volume. We used femtosecond laser pulses of 800 nm and energy between 25 and 123 μJ to generate LIBS signals in biological tissues. As expected, we observed a linear increase in the fsLIBS intensity as a function of the laser energy. In addition, we show that moving the beam out of focus and the presence of overlapping pulses on the same focal area leads to a decrease in fsLIBS intensity due to changes in focal spot size. We also demonstrate that fsLIBS can distinguish between different biological tissue samples.
authors.affiliation: Center for Biophotonics Science and Technology, University of California, Davis, CA 95616 USA.
authors.forename: Ruby K
authors.initials: RK
authors.lastname: Gill
authors.forename: Florian
authors.initials: F
authors.lastname: Knorr
authors.forename: Zachary J
authors.initials: ZJ
authors.lastname: Smith
authors.forename: Mehmet
authors.initials: M
authors.lastname: Kahraman
authors.forename: Dorte
authors.initials: D
authors.lastname: Madsen
authors.forename: Delmar S
authors.initials: DS
authors.lastname: Larsen
authors.forename: Sebastian
authors.initials: S
authors.lastname: Wachsmann-Hogiu
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000737:chemistry; D002356:Cartilage / Q000737:chemistry; D002417:Cattle; D002645:Chickens; D004528:Egg Shell / Q000737:chemistry; D007834:Lasers; D008853:Microscopy; D013057:Spectrum Analysis / Q000379:methods*