title: Role of gastric acid inhibition, prostaglandins and endogenous-free thiol groups on the gastroprotective effect of a proteolytic fraction from Vasconcellea cundinamarcensis latex.
abstract: OBJECTIVE
  The aim of this study was to extend our knowledge about the mechanism involved in the gastroprotective effect of P1G10, a proteolytic fraction rich in cysteine proteinases from Vasconcellea cundinamarcensis (syn. Carica candamarcensis) latex, which demonstrated gastric healing and protection activities in rats.
  
  
  METHODS
  Wistar rats were submitted to gastric lesions by indomethacin and treated with P1G10 (10 mg/kg). Free thiol groups and prostaglandin E2 content were measured in gastric mucosal and gastrin levels in blood samples. To evaluate the participation of nitric oxide (NO) or proteolytic activity of P1G10 on its gastroprotective effect, animals were treated with an inhibitor of NO production (L-NAME) or the fraction inhibited by iodoacetamide, respectively. Gastric secretion study (acidity and pepsin activity) was also performed.
  
  
  RESULTS
  P1G10 (10 mg/kg) inhibited the occurrence of gastric lesions by indomethacin, restored the free thiol groups content on gastric mucosa and increased moderately prostaglandin E2 levels (34%). Furthermore, the treatment decreased the gastrin levels (95%), suggesting a possible modulation of secretory activity. This effect was accordant with attenuation of gastric acidity (42%) and pepsin activity (69%) seen in animals subjected to pyloric ligation. The inhibition of NO production or the proteolytic activity of P1G10 does not affect the gastroprotective effect.
  
  
  CONCLUSIONS
  These results can explain the gastroprotective activity of P1G10 and serve a basis for further studies of this active principle.
authors.affiliation: Departamento de Farmacologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Ana Candida
authors.initials: AC
authors.lastname: Araujo e Silva
authors.forename: Fernanda
authors.initials: F
authors.lastname: de Oliveira Lemos
authors.forename: Marco Túlio Ribeiro
authors.initials: MT
authors.lastname: Gomes
authors.forename: Carlos Edmundo
authors.initials: CE
authors.lastname: Salas
authors.forename: Miriam Teresa Paz
authors.initials: MT
authors.lastname: Lopes
mesh_terms: D000818:Animals; D029441:Carica; D057056:Cysteine Proteases / Q000494:pharmacology*; D015232:Dinoprostone / Q000378:metabolism*; D005260:Female; D005744:Gastric Acid / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D005753:Gastric Mucosa; D005755:Gastrins / Q000096:biosynthesis / Q000097:blood; D007213:Indomethacin / Q000494:pharmacology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000378:metabolism*
chemical_list: D005755:Gastrins; D010936:Plant Extracts; D013438:Sulfhydryl Compounds; D009569:Nitric Oxide; D057056:Cysteine Proteases; D015232:Dinoprostone; D019331:NG-Nitroarginine Methyl Ester; D007213:Indomethacin
keywords: Carica candamarcensis; NSAIDs; Vasconcellea cundinamarcensis; cysteine proteinases; gastric protection