title: Paradevosia shaoguanensis gen. nov., sp. nov., isolated from a coking wastewater.
abstract: A Gram staining negative, rod-shaped, aerobic bacterial strain J5-3(T) with a single polar flagellum was isolated from coking wastewater collected from Shaoguan, Guangdong, China. It was motile and capable of optimal growth at pH 6-8, 30 °C, and 0-2 % (w/v) NaCl. Its predominant fatty acids were 11-methyl C18:1 ω7c (29.2 %), C16:0 (20.6 %), C19:0 cyclo ω8c (18.2 %), C18:0 (11.0 %), and C18:1 ω7c/C18:1 ω6c (10.9 %) when grown on trypticase soy agar. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids (GL1, GL2), and two unknown phospholipid (PL1, PL2). The predominant ubiquinone was Q-10, and the genome DNA G+C content was 61.7 mol %. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain J5-3(T) belonged to the family Hyphomicrobiaceae in Alphaproteobacteria. It shared the 16S rRNA gene sequence similarities of 93.8-96.1 % with the genus Devosia, 94.5-94.8 % with the genus Pelagibacterium, and <92.0 % with all the other type strains in family Hyphomicrobiaceae. It can be distinguished from the closest phylogenetic neighbors based on several phenotypic and genotypic features, including α-galactosidase activity, tetracycline susceptibility, major fatty acid composition, polar lipid profile, DNA gyrase B subunit (gyrB) gene sequence, and random-amplified polymorphic DNA profile. Therefore, we consider strain J5-3(T) to represent a novel species of a novel genus within the family Hyphomicrobiaceae, for which the name Paradevosia shaoguanensis gen. nov., sp. nov. is proposed. The type strain of Paradevosia shaoguanensis is J5-3(T) (=CGMCC 1.12430(T) =LMG 27409(T)).
authors.affiliation: College of Engineering, Peking University, Beijing, 100871, People's Republic of China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Geng
authors.forename: Xin-Chi
authors.initials: XC
authors.lastname: Pan
authors.forename: Ran
authors.initials: R
authors.lastname: Mei
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Wang
authors.forename: Ji-Quan
authors.initials: JQ
authors.lastname: Sun
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Liu
authors.forename: Yue-Qin
authors.initials: YQ
authors.lastname: Tang
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Wu
mesh_terms: D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D041921:Hyphomicrobiaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D007220:Industrial Waste / Q000032:analysis*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D062065:Wastewater / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; D062065:Wastewater