title: Secretoglobin superfamily protein SCGB3A2 deficiency potentiates ovalbumin-induced allergic pulmonary inflammation.
abstract: Secretoglobin (SCGB) 3A2, a cytokine-like secretory protein of small molecular weight, which may play a role in lung inflammation, is predominantly expressed in airway epithelial cells. In order to understand the physiological role of SCGB3A2, Scgb3a2(-/-) mice were generated and characterized. Scgb3a2(-/-) mice did not exhibit any overt phenotypes. In ovalbumin- (OVA-) induced airway allergy inflammation model, Scgb3a2(-/-) mice in mixed background showed a decreased OVA-induced airway inflammation, while six times C57BL/6NCr backcrossed congenic Scgb3a2(-/-) mice showed a slight exacerbation of OVA-induced airway inflammation as compared to wild-type littermates. These results indicate that the loss of SCGB3A2 function was influenced by a modifier gene(s) in mixed genetic background and suggest that SCGB3A2 has anti-inflammatory property. The results further suggest the possible use of recombinant human SCGB3A2 as an anti-inflammatory agent.
authors.affiliation: Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Building 37, Room 3106, NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA ; Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.
authors.forename: Taketomo
authors.initials: T
authors.lastname: Kido
authors.affiliation: Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Building 37, Room 3106, NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA.
authors.forename: Mitsuhiro
authors.identifier: 0000-0001-6340-1205
authors.initials: M
authors.lastname: Yoneda
authors.affiliation: Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Building 37, Room 3106, NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Building 37, Room 3106, NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA ; Department of Anatomy, Graduate School of Medicine, Osaka City University, Osaka 545-8585, Japan.
authors.forename: Tsutomu
authors.identifier: 0000-0003-1100-1052
authors.initials: T
authors.lastname: Matsubara
authors.affiliation: Global VetPathology, Montgomery Village, MD 20866, USA.
authors.forename: Jerrold M
authors.initials: JM
authors.lastname: Ward
authors.affiliation: Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Building 37, Room 3106, NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA.
authors.forename: Shioko
authors.identifier: 0000-0001-9627-6818
authors.initials: S
authors.lastname: Kimura
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018793:Interleukin-13 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D051379:Mice; D010047:Ovalbumin / Q000494:pharmacology*; D011014:Pneumonia / Q000139:chemically induced* / Q000378:metabolism*; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D060765:Secretoglobins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*
chemical_list: D018793:Interleukin-13; D015848:Interleukin-5; C449818:Scgb3a2 protein, mouse; D060765:Secretoglobins; D015847:Interleukin-4; D010047:Ovalbumin