title: Selection of target mutation in rat gastrointestinal tract E. coli by minute dosage of enrofloxacin.
abstract: It has been suggested that bacterial resistance is selected within a mutation selection window of antibiotics. More recent studies showed that even extremely low concentration of antibiotic could select resistant bacteria in vitro. Yet little is known about the exact antibiotic concentration range that can effectively select for resistant organisms in animal gastrointestinal (GI) tract. In this study, the effect of different dosages of enrofloxacin on resistance and mutation development in rat GI tract E. coli was investigated by determining the number of resistant E. coli recoverable from rat fecal samples. Our data showed that high dose antibiotic treatment could effectively eliminate E. coli with single gyrA mutation in the early course of treatment, yet the eradication effects diminished upon prolonged treatment. Therapeutic and sub-therapeutic dose (1/10 and 1/100 of therapeutic doses) of enrofloxacin could effectively select for mutation in GI tract E. coli at the later course of enrofloxacin treatment and during the cessation periods. Surprisingly, very low dose of enrofloxacin (1/1000 therapeutic dose) could also select for mutation in GI tract E. coli at the later course of enrofloxacin treatment, only with slightly lower efficiency. No enrofloxacin-resistant E. coli could be selected at all test levels of enrofloxacin during long term treatment and the strength of antibiotic treatment does not alter the overall level of E. coli in rat GI tract. This study demonstrated that long term antibiotic treatment seems to be the major trigger for the development of target mutations in GI tract E. coli, which provided insight into the rational use of antibiotics in animal husbandry.
authors.affiliation: Food Safety and Technology Research Center, Hong Kong Polytechnic University - Shen Zhen Research Institute Shenzhen, China ; The State Key Lab of Chiroscience, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University Kowloon, Hong Kong.
authors.forename: Dachuan
authors.initials: D
authors.lastname: Lin
authors.affiliation: Food Safety and Technology Research Center, Hong Kong Polytechnic University - Shen Zhen Research Institute Shenzhen, China ; The State Key Lab of Chiroscience, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University Kowloon, Hong Kong.
authors.forename: Kaichao
authors.initials: K
authors.lastname: Chen
authors.affiliation: Food Safety and Technology Research Center, Hong Kong Polytechnic University - Shen Zhen Research Institute Shenzhen, China ; The State Key Lab of Chiroscience, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University Kowloon, Hong Kong.
authors.forename: Ruichao
authors.initials: R
authors.lastname: Li
authors.affiliation: Food Safety and Technology Research Center, Hong Kong Polytechnic University - Shen Zhen Research Institute Shenzhen, China ; The State Key Lab of Chiroscience, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University Kowloon, Hong Kong.
authors.forename: Lizhang
authors.initials: L
authors.lastname: Liu
authors.affiliation: Food Safety and Technology Research Center, Hong Kong Polytechnic University - Shen Zhen Research Institute Shenzhen, China ; The State Key Lab of Chiroscience, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University Kowloon, Hong Kong.
authors.forename: Jiubiao
authors.initials: J
authors.lastname: Guo
authors.affiliation: College of Animal Science and Technology, Nanjin Agriculture University Nanjin, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Yao
authors.affiliation: Food Safety and Technology Research Center, Hong Kong Polytechnic University - Shen Zhen Research Institute Shenzhen, China ; The State Key Lab of Chiroscience, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University Kowloon, Hong Kong.
authors.forename: Sheng
authors.initials: S
authors.lastname: Chen
keywords: enrofloxacin; rat GI tract E. coli; resistance development; target mutation