title: The MAT1-1:MAT1-2 ratio of Sporothrix globosa isolates in Japan.
abstract: In order to understand the reproductive biology of pathogenic species in the Sporothrix schenckii complex, we characterized the partial mating type (MAT1-1) loci of Sporothrix schenckii, as well as the S. globosa MAT1-1-1 gene, which encoded 262 amino acid sequences. The data confirmed that the MAT1-1 locus of S. globosa was divergent from the MAT1-2 locus of the opposite mating type, suggesting that the fungus is heterothallic. To determine the mating type ratio of 20 isolates from Japanese patients, we analyzed the MAT loci by specific PCR amplification of MAT1-1-1 and MAT1-2-1 genes. The MAT1-1-1 was detected in 5 isolates but not in the other 15 isolates with the presence of MAT1-2-1. The MAT1-1:1-2 ratio of S. globosa isolates in Japan was estimated to be 1:3. Phylogenetic analysis indicated that the sequences of the MAT1-1-1 were identical among S. globosa isolates but different from S. schenckii and Ophiostoma montium.
authors.affiliation: Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866 Kameino, Fujisawa, Kanagawa, 252-8510, Japan, kanou.rui@nihon-u.ac.jp.
authors.forename: Rui
authors.initials: R
authors.lastname: Kano
authors.forename: Clement K-M
authors.initials: CK
authors.lastname: Tsui
authors.forename: Richard C
authors.initials: RC
authors.lastname: Hamelin
authors.forename: Kazushi
authors.initials: K
authors.lastname: Anzawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Mochizuki
authors.forename: Katsutaro
authors.initials: K
authors.lastname: Nishimoto
authors.forename: Masataro
authors.initials: M
authors.lastname: Hiruma
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kamata
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hasegawa
mesh_terms: D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D049770:Genes, Mating Type, Fungal / Q000235:genetics*; D006801:Humans; D007564:Japan; D010802:Phylogeny; D012098:Reproduction / Q000235:genetics* / Q000502:physiology; D017422:Sequence Analysis, DNA; D013175:Sporothrix / Q000235:genetics* / Q000302:isolation & purification; D013174:Sporotrichosis / Q000382:microbiology
chemical_list: D004271:DNA, Fungal