title: Lentivirus-mediated knockdown of Krüppel-like factor 9 inhibits the growth of ovarian cancer.
abstract: OBJECTIVE
  Ovarian cancer is among the top diseases in the list of malignant gynaecologic tumors. In the present study, we aim to investigate the effect of lentivirus-mediated knockdown of Krüppel-like factor 9 (KLF9) on cell viability and tumor growth in ovarian cancer.
  
  
  METHODS
  Firstly, the expression of KLF9 was determined by real-time PCR and western blot in human ovarian cancer tissues. Then, endogenous KLF9 expression was silenced by lentivirus in SKOV3 and OVCAR3 ovarian cancer cells, and followed by MTT and BrdU incorporation assays, cell cycle analysis and tumor xenografts in nude mice.
  
  
  RESULTS
  Our results found that the expression of KLF9 is up-regulated in human ovarian cancer. As expected, KLF9 knockdown significantly inhibited cell proliferation and resulted in cell cycle arrest in the G0/G1 phase. Besides, KLF9 deficiency significantly inhibited tumor growth in nude mice.
  
  
  CONCLUSIONS
  Therefore, our data reveal that lentivirus-mediated KLF9 silencing might be promising in the treatment of human ovarian cancer.
authors.affiliation: Department of Gynaecology, Central Hospital of Zibo, Zibo, 255000, Shandong, China.
authors.forename: Qing-hua
authors.initials: QH
authors.lastname: Zhang
authors.forename: Hong-tao
authors.initials: HT
authors.lastname: Dou
authors.forename: Yu-jie
authors.initials: YJ
authors.lastname: Tang
authors.forename: Shan
authors.initials: S
authors.lastname: Su
authors.forename: Pei-shu
authors.initials: PS
authors.lastname: Liu
mesh_terms: D000818:Animals; D059447:Cell Cycle Checkpoints / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000235:genetics*; D016086:Lentivirus / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology*; D034622:RNA Interference; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C489676:KLF9 protein, human; D051741:Kruppel-Like Transcription Factors