title: Polaribacter marinivivus sp. nov., a member of the family Flavobacteriaceae isolated from seawater.
abstract: A Gram-stain negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated GYSW-15(T), was isolated from a seawater of Geoje island on the South Sea, South Korea, and subjected to a polyphasic taxonomic approach. Strain GYSW-15(T) was found to grow optimally at 30 °C, at pH 7.0-8.0 and in the presence of approximately 2.0 % (w/v) NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain GYSW-15(T) belongs to the genus Polaribacter, clustering coherently with the type strain of Polaribacter porphyrae showing sequence similarity value of 97.0 %. Strain GYSW-15(T) exhibited 16S rRNA gene sequence similarity values of 94.3-96.4 % to the type strains of the other Polaribacter species. Strain GYSW-15(T) was found to contain MK-6 as the predominant menaquinone and iso-C15:0, iso-C15:1 G, iso-C16:0 3-OH and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) as the major fatty acids. The polar lipid profile of strain GYSW-15(T) containing phosphatidylethanolamine and an unidentified lipid as major components was similar to that of the type strain of P. porphyrae. The DNA G + C content of strain GYSW-15(T) was determined to be 31.2 mol% and its mean DNA-DNA relatedness with P. porphyrae LMG 26671(T) was 17 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain GYSW-15(T) is separated from recognized Polaribacter species. On the basis of the data presented, strain GYSW-15(T) is considered to represent a novel species of the genus Polaribacter, for which the name Polaribacter marinivivus sp. nov. is proposed. The type strain is GYSW-15(T) ( = KCTC 42156(T) = CECT 8655(T)).
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Park
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.forename: Keun-Chul
authors.initials: KC
authors.lastname: Lee
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Lee
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012965:Sodium Chloride