title: Drosophila protamine-like Mst35Ba and Mst35Bb are required for proper sperm nuclear morphology but are dispensable for male fertility.
abstract: During spermiogenesis, histones are massively replaced with protamines. A previous report showed that Drosophila males homozygous for a genomic deletion covering several genes including the protamine-like genes Mst35Ba/b are surprisingly fertile. Here, we have precisely deleted the Mst35B locus by homologous recombination, and we confirm the dispensability of Mst35Ba/b for fertility.
authors.affiliation: Centre de Génétique et de Physiologie Moléculaire et Cellulaire - CNRS UMR 5534 - Université Claude Bernard Lyon1, 69100 Villeurbanne, France.
authors.forename: Samantha
authors.initials: S
authors.lastname: Tirmarche
authors.affiliation: Centre de Génétique et de Physiologie Moléculaire et Cellulaire - CNRS UMR 5534 - Université Claude Bernard Lyon1, 69100 Villeurbanne, France.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Kimura
authors.affiliation: Centre de Génétique et de Physiologie Moléculaire et Cellulaire - CNRS UMR 5534 - Université Claude Bernard Lyon1, 69100 Villeurbanne, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Sapey-Triomphe
authors.affiliation: Department of Molecular, Cell, and Developmental Biology, University of California Santa Cruz, Santa Cruz, California 95064.
authors.forename: William
authors.initials: W
authors.lastname: Sullivan
authors.affiliation: Centre de Recherche de Biochimie Macromoléculaire - CNRS UMR 5237 - 34293 Montpellier, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Landmann
authors.affiliation: Centre de Génétique et de Physiologie Moléculaire et Cellulaire - CNRS UMR 5534 - Université Claude Bernard Lyon1, 69100 Villeurbanne, France benjamin.loppin@univ-lyon1.fr.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Loppin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004330:Drosophila / Q000235:genetics* / Q000502:physiology; D029721:Drosophila Proteins / Q000235:genetics*; D017353:Gene Deletion; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D011479:Protamines / Q000235:genetics*; D013094:Spermatozoa / Q000166:cytology*
chemical_list: D029721:Drosophila Proteins; C503325:ProtA protein, Drosophila; C503324:ProtB protein, Drosophila; D011479:Protamines
keywords: Drosophila; Mst35B; protamine-like; sperm; spermiogenesis