title: pH and DNA luminescence switching, DNA photocleavage and cytotoxic properties of two thiophene-containing ruthenium(II) complexes.
abstract: Two new Ru(II) complexes, [Ru(Htip)3]Cl2 (1) and [Ru(Htip)2(dppz)]Cl2 (2), were synthesised and were characterised. The ground- and excited-state acid-base properties of 1 and 2 were studied and demonstrated that 1 acted as a pH-induced "on-off-on" luminescence switch. The binding behaviours of 1 and 2 to calf thymus DNA were studied with absorption and emission spectroscopy, DNA viscosities and density functional theory calculations. 2 was found to act as a DNA molecular light switch and as an efficient photocleaver of pUC 18 DNA. The cytotoxicities of the complexes were evaluated with the MTT method and it was found that 1 displayed apparent anticancer activity against MCF-7 cell, whereas 2 exhibited more potent and wider-spectrum antitumor activities against all cancer cell lines tested.
authors.affiliation: Beijing Key Laboratory of Energy Conversion and Storage Materials, College of Chemistry, Beijing Normal University, Beijing 100875, PR China; College of Chemistry and Environmental Science, Hebei University, Baoding 071002, PR China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Zhao
authors.affiliation: Beijing Key Laboratory of Energy Conversion and Storage Materials, College of Chemistry, Beijing Normal University, Beijing 100875, PR China.
authors.forename: Zhen-Sheng
authors.initials: ZS
authors.lastname: Li
authors.affiliation: Beijing Key Laboratory of Energy Conversion and Storage Materials, College of Chemistry, Beijing Normal University, Beijing 100875, PR China.
authors.forename: An-Guo
authors.initials: AG
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of Energy Conversion and Storage Materials, College of Chemistry, Beijing Normal University, Beijing 100875, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Baoding 071002, PR China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Song
authors.affiliation: Beijing Key Laboratory of Energy Conversion and Storage Materials, College of Chemistry, Beijing Normal University, Beijing 100875, PR China. Electronic address: kzwang@bnu.edu.cn.
authors.forename: Ke-Zhi
authors.initials: KZ
authors.lastname: Wang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects* / Q000528:radiation effects; D056831:Coordination Complexes / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects; D053837:DNA Cleavage / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D049449:Luminescence; D015394:Molecular Structure; D009369:Neoplasms / Q000188:drug therapy; D009942:Organometallic Compounds / Q000737:chemistry / Q000494:pharmacology*; D011789:Quantum Theory; D012428:Ruthenium / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013876:Thiophenes / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D009942:Organometallic Compounds; D013876:Thiophenes; D012428:Ruthenium; D004247:DNA; C427203:calf thymus DNA
keywords: Cytotoxicity; DNA; DNA photocleavage; DNA switch; Ruthenium