title: Quantitative analysis of pungent and anti-inflammatory phenolic compounds in olive oil by capillary electrophoresis.
abstract: The first CE procedure for the quantitative determination of pharmacologically relevant secoiridoids in olive oil, oleocanthal and oleacein, is described. Together with their precursors tyrosol and hydroxytyrosol they could be baseline separated in less than 15min using a borax buffer with pH 9.5, at 25kV and 30°C. Method validation confirmed that the procedure is selective, accurate (recovery rates from 94.0 to 104.6%), reproducible (σmax⩽6.8%) and precise (inter-day precision⩽6.4%), and that the compounds do not degrade quickly if non-aqueous acetonitrile is used as solvent. Quantitative results indicated a low occurrence of oleocanthal (0.004-0.021%) and oleacein (0.002-0.048%) in olive oil samples, which is in agreement to published HPLC data. The CE method impresses with its simple instrumental and methodological design, combined with reproducible and valid quantitative results.
authors.affiliation: Institute of Pharmacy, Pharmacognosy, Member of CMBI, University of Innsbruck, 6020 Innsbruck, Austria.
authors.forename: Isabella
authors.initials: I
authors.lastname: Vulcano
authors.affiliation: Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, Athens 15771, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Halabalaki
authors.affiliation: Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, Athens 15771, Greece.
authors.forename: Leandros
authors.initials: L
authors.lastname: Skaltsounis
authors.affiliation: Institute of Pharmacy, Pharmacognosy, Member of CMBI, University of Innsbruck, 6020 Innsbruck, Austria. Electronic address: Markus.Ganzera@uibk.ac.at.
authors.forename: Markus
authors.initials: M
authors.lastname: Ganzera
mesh_terms: D000447:Aldehydes / Q000032:analysis; D000893:Anti-Inflammatory Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D000081004:Cyclopentane Monoterpenes; D019075:Electrophoresis, Capillary / Q000379:methods*; D000069463:Olive Oil / Q000032:analysis*; D010636:Phenols / Q000032:analysis*
chemical_list: D000447:Aldehydes; D000893:Anti-Inflammatory Agents; D000081004:Cyclopentane Monoterpenes; D000069463:Olive Oil; D010636:Phenols; C578055:oleacein; C503534:oleocanthal
keywords: Capillary electrophoresis; Hydroxytyrosol (PubChem CID: 82755); Inflammation; Oleacein; Oleacein (no PubChem CID, CAS: 149183-75-5); Oleocanthal; Oleocanthal (PubChem CID: 16681728); Olive oil; Phenolic compounds; Tyrosol (PubChem CID: 10393)