title: Microbiota in the Throat and Risk Factors for Laryngeal Carcinoma.
abstract: The compositions and abundances of the microbiota in the ecological niche of the human throat and the possible relationship between the microbiota and laryngeal cancer are poorly understood. To obtain insight into this, we enrolled 27 laryngeal carcinoma patients and 28 subjects with vocal cord polyps as controls. For each subject, we simultaneously collected swab samples from the upper throat near the epiglottis (site I) and tissue samples from the vestibulum laryngis to the subglottic region (site II). The microbiota of the throat were fully characterized by pyrosequencing of barcoded 16S rRNA genes. We found 14 phyla, 20 classes, 38 orders, 85 families, and 218 genera in the throats of enrolled subjects. The main phyla were Firmicutes (54.7%), Fusobacteria (14.8%), Bacteroidetes (12.7%), and Proteobacteria (10.6%). Streptococcus (37.3%), Fusobacterium (11.3%), and Prevotella (10.6%) were identified as the three most predominant genera in the throat. The relative abundances of 23 bacterial genera in site I were significantly different from those in site II (P < 0.05). The relative proportions of 12 genera largely varied between laryngeal cancer patients and control subjects (P < 0.05). Collectively, this study outlined the spatial structure of microbial communities in the human throat. The spatial structure of bacterial communities significantly varied in two anatomical sites of the throat. The bacterial profiles of the throat of laryngeal cancer patients were strongly different from those of control subjects, and several of these microorganisms may be related to laryngeal carcinoma.
authors.affiliation: Department of Otolaryngology, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Gong
authors.affiliation: Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, China yishi750@163.com zhoulent@126.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Biological Sciences, University of Idaho, Moscow, Idaho, USA.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Otolaryngology, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Otolaryngology, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, China.
authors.forename: Pengyu
authors.initials: P
authors.lastname: Cao
authors.affiliation: Shanghai Key Laboratory for Reproductive Medicine, Department of Histology and Embryology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Xu
authors.affiliation: Shanghai Key Laboratory for Reproductive Medicine, Department of Histology and Embryology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Hou
authors.affiliation: Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Sequencing Centre, Shanghai, China.
authors.forename: Yuezhu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, China yishi750@163.com zhoulent@126.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D058448:Biota; D002277:Carcinoma / Q000453:epidemiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006801:Humans; D007822:Laryngeal Neoplasms / Q000453:epidemiology*; D008969:Molecular Sequence Data; D010614:Pharynx / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012307:Risk Factors; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S