title: Expression of heat shock protein 70 in lung tissues of acute paraquat poisoned rats and intervention of ulinastatin.
abstract: BACKGROUND
  Paraquat (PQ) is an effective herbicide and is widely used in agricultural production, but PQ poisoning is frequently seen in humans with the lung as the target organ. Clinically pulmonary pathological changes are often used to predict the severity and prognosis of the patients. In this study, we observed the expression of heat shock protein 70 (HSP70) in rat lung after PQ poisoning and to investigate the therapeutic effects of ulinastatin.
  
  
  METHODS
  Seventy-two adult healthy SD rats were randomly divided into a control group (group A, n=24), a poisoning group (group B, n=24), and an ulinastatin group (group C, n=24). The rat models of acute PQ poisoning were established by intra-gastric administration of 80 mg/kg PQ to rats of groups B and C, and the rats of group C were intra-peritoneally injected with 100 000 IU/kg ulinastatin 30 minutes after poisoning. The expression of HSP70 in lung tissue was observed, and W/D and histopathological changes in the lung tissue were compared 12, 24, 48 and 72 hours after poisoning. The expression of HSP70 in the lung tissue was assayed by using RT-PCR. All quantitative data were processed with one-way analysis of variance to compare multiple sample means.
  
  
  RESULTS
  Compared to group A, the expression of HSP70 in the lung of rats in groups B and C increased significantly at all intervals (P<0.05). The pathological changes in lung tissue of rats with PQ poisoning included congestion, leukocytes infiltration and local hemorrhage, whereas those of group C were significantly lessened.
  
  
  CONCLUSIONS
  Ulinastatin may ameliorate acute lung injury to some extent after PQ poisoning in rats by enhancing the expression of HSP70.
authors.affiliation: Neurosurgery ICU, Banan District People's Hospital, Chongqing 401320, China (Zhang ZJ); Emergemcy Medicine Department, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China (Zhou CY, Luo YJ, Xiong HW).
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Zhang
authors.affiliation: Neurosurgery ICU, Banan District People's Hospital, Chongqing 401320, China (Zhang ZJ); Emergemcy Medicine Department, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China (Zhou CY, Luo YJ, Xiong HW).
authors.forename: Cong-Yang
authors.initials: CY
authors.lastname: Zhou
authors.affiliation: Neurosurgery ICU, Banan District People's Hospital, Chongqing 401320, China (Zhang ZJ); Emergemcy Medicine Department, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China (Zhou CY, Luo YJ, Xiong HW).
authors.forename: Ya-Juan
authors.initials: YJ
authors.lastname: Luo
authors.affiliation: Neurosurgery ICU, Banan District People's Hospital, Chongqing 401320, China (Zhang ZJ); Emergemcy Medicine Department, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China (Zhou CY, Luo YJ, Xiong HW).
authors.forename: Hua-Wei
authors.initials: HW
authors.lastname: Xiong
keywords: Acute lung injury; Heat shock protein; Paraquat; Poisoning; Ulilnastatin