title: Transcriptional response to the [ISP(+) ] prion of Saccharomyces cerevisiae differs from that induced by the deletion of its structural gene, SFP1.
abstract: Currently, several protein-based genetic determinants, or prions, are described in yeast, and several hundred prion candidates have been predicted. Importantly, many known and potential prion proteins regulate transcription; therefore, prion induction should affect gene expression. While it is generally believed that the prion phenotype should mimic the deletion phenotype, this rule has exceptions. Formed by the transcription factor Sfp1p, [ISP(+) ] is one such exception as the [ISP(+) ] and sfp1Δ strains differ in many phenotypic traits. These data suggest that effects of prion formation by a transcription factor and its absence may affect gene expression in a different way. However, studies addressing this issue are practically absent. Here, we explore how [ISP(+) ] affects gene expression and how these changes correspond to the effect of SFP1 deletion. Our data indicate that the [ISP(+) ]-related expression changes cannot be explained by the inactivation of Sfp1p. Remarkably, most Sfp1p targets are not affected in the [ISP(+) ] strain; instead, the genes upregulated in the [ISP(+) ] strain are enriched in Gcn4p and Aft1p targets. We propose that Sfp1p serves as a part of a regulatory complex, and the activity of this complex may be modulated differently by the absence or prionization of Sfp1p.
authors.affiliation: Department of Genetics and Biotechnology, Saint Petersburg State University, St. Petersburg, Russia; Laboratory of Amyloid Biology, Saint Petersburg State University, St. Petersburg, Russia.
authors.forename: Polina
authors.initials: P
authors.lastname: Drozdova
authors.forename: Tatyana
authors.initials: T
authors.lastname: Rogoza
authors.forename: Elina
authors.initials: E
authors.lastname: Radchenko
authors.forename: Polina
authors.initials: P
authors.lastname: Lipaeva
authors.forename: Ludmila
authors.initials: L
authors.lastname: Mironova
mesh_terms: D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D011328:Prions / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D017384:Sequence Deletion; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D011328:Prions; C117421:SFP1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins
keywords: microarray; whole genome expression profile; yeast prion