title: SCYL1BP1 has tumor-suppressive functions in human lung squamous carcinoma cells by regulating degradation of MDM2.
abstract: SCY1-like 1-binding protein 1 (SCYL1BP1) is a newly identified transcriptional activator domain containing protein with many unknown biological functions. Recently emerging evidence has revealed that it is a novel regulator of the p53 pathway, which is very important for the development of human cancer. However, the effects of SCYL1BP1 on human lung squamous carcinoma cell biological behavior remain poorly understood. In this study, we present evidence that SCYL1BP1 can promote the degradation of MDM2 protein and further inhibit the G1/S transition of lung squamous carcinoma cell lines. Functional assays found that reintroduction of SCYL1BP1 into lung squamous carcinoma cell lines significantly inhibited cell proliferation, migration, invasion and tumor formation in nude mice, suggesting strong tumor suppressive function of SCYL1BP1 in lung squamous carcinoma. Taken together, our data suggest that the interaction of SCYL1BP1/MDM2 could accelerate MDM2 degradation, and may function as an important tumor suppressor in lung squamous carcinomas.
authors.affiliation: Department of Oncology and Pathology, The First Hospital of Jiaxing, Jiaxing, China E-mail : wanxinwu@outlook.com.
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Yang
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Xie
authors.forename: Dan-Yan
authors.initials: DY
authors.lastname: Ling
authors.forename: Jin-Rui
authors.initials: JR
authors.lastname: Li
authors.forename: Jin
authors.initials: J
authors.lastname: Jiang
authors.forename: Yao-Hua
authors.initials: YH
authors.lastname: Fan
authors.forename: Jia-Lian
authors.initials: JL
authors.lastname: Zheng
authors.forename: Wan-Xin
authors.initials: WX
authors.lastname: Wu
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D059585:G1 Phase Cell Cycle Checkpoints / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D000077614:Golgi Matrix Proteins; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D051736:Proto-Oncogene Proteins c-mdm2 / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; C533680:GORAB protein, human; D000077614:Golgi Matrix Proteins; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2