title: Rapid and efficient elimination of specific nuclear noncoding RNAs in mammalian cells with antisense oligonucleotides.
abstract: A wide variety of noncoding RNAs are thought to play fundamental and regulatory functions in the mammalian cell nucleus, although their primary functions have yet to be elucidated. Here, we describe a method for the rapid and efficient elimination of specific nuclear noncoding RNAs in mammalian cells using chemically modified antisense oligonucleotides, which may facilitate the functional assessment of nuclear ncRNAs.
authors.affiliation: Institute for Genetic Medicine, Hokkaido University, Kita-15 Nishi-7, Kita-ku, Sapporo, 060-0815, Japan, hirose@igm.hokudai.ac.jp.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Hirose
authors.forename: Taro
authors.initials: T
authors.lastname: Mannen
mesh_terms: D006367:HeLa Cells; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis; D022661:RNA, Untranslated; D014162:Transfection / Q000379:methods
chemical_list: D016376:Oligonucleotides, Antisense; D022661:RNA, Untranslated