title: Effects of Nicotine on Streptococcus gordonii Growth, Biofilm Formation, and Cell Aggregation.
abstract: Streptococcus gordonii is a commensal species of human oral flora. It initiates dental biofilm formation and provides binding sites for later colonizers to attach to and generate mature biofilm. Smoking is the second highest risk factor for periodontal disease, and cigarette smoke extract has been reported to facilitate Porphyromonas gingivalis-S. gordonii dual-species biofilm formation. Our hypothesis is that nicotine, one of the most important and active components of tobacco, stimulates S. gordonii multiplication and aggregation. In the present study, S. gordonii planktonic cell growth (kinetic absorbance and CFU), biofilm formation (crystal violet stain and confocal laser scanning microscopy [CLSM]), aggregation with/without sucrose, and 11 genes that encode binding proteins or regulators of gene expression were investigated. Results demonstrated planktonic cell growth was stimulated by 1 to 4 mg/ml nicotine treatment. Biofilm formation was increased at 0.5 to 4 mg/ml nicotine. CLSM indicated bacterial cell mass was increased by 2 and 4 mg/ml nicotine, but biofilm extracellular polysaccharide was not significantly affected by nicotine. Cell aggregation was upregulated by 4, 8, and 16 mg/ml nicotine with sucrose and by 16 mg/ml nicotine without sucrose. Quantitative reverse transcriptase PCR indicated S. gordonii abpA, scaA, ccpA, and srtA were upregulated in planktonic cells by 2 mg/ml nicotine. In conclusion, nicotine stimulates S. gordonii planktonic cell growth, biofilm formation, aggregation, and gene expression of binding proteins. Those effects may promote later pathogen attachment to tooth surfaces, the accumulation of tooth calculus, and the development of periodontal disease in cigarette smokers.
authors.affiliation: Department of Oral Biology and Tobacco Cessation and Biobehavioral Group, School of Dentistry, Indiana University, Indianapolis, Indiana, USA State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: R
authors.initials: R
authors.lastname: Huang
authors.affiliation: Department of Oral Biology and Tobacco Cessation and Biobehavioral Group, School of Dentistry, Indiana University, Indianapolis, Indiana, USA State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, Indiana University, Indianapolis, Indiana, USA.
authors.forename: M
authors.initials: M
authors.lastname: Ye
authors.affiliation: Department of Electrical and Computer Engineering, Indiana University-Purdue University, Indianapolis, Indiana, USA.
authors.forename: K
authors.initials: K
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: X
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Oral Biology and Tobacco Cessation and Biobehavioral Group, School of Dentistry, Indiana University, Indianapolis, Indiana, USA Department of Pathology and Laboratory Medicine, School of Medicine, Indiana University, Indianapolis, Indiana, USA rgregory@iu.edu.
authors.forename: R L
authors.initials: RL
authors.lastname: Gregory
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D015169:Colony Count, Microbial; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D018613:Microscopy, Confocal; D009538:Nicotine / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013053:Spectrophotometry; D013194:Staining and Labeling; D054773:Streptococcus gordonii / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology*; D014026:Nicotiana
chemical_list: D018829:Adhesins, Bacterial; D009538:Nicotine