title: A plasmon-assisted fluoro-immunoassay using gold nanoparticle-decorated carbon nanotubes for monitoring the influenza virus.
abstract: A plasmon-assisted fluoro-immunoassay (PAFI) was developed for the detection of the influenza virus by using Au nanoparticle (Au NP)-decorated carbon nanotubes (AuCNTs) that were synthesized using phytochemical composites at room temperature in deionized water. Specific antibodies (Abs) against the influenza virus were conjugated onto the surface of AuCNTs and cadmium telluride quantum dots (QDs), which had a photoluminescence intensity that varied as a function of virus concentration and a detection limit of 0.1 pg/mL for all three types of influenza viruses examined. The clinically isolated influenza viruses (A/Yokohama/110/2009 (H3N2)) were detected in the range of 50-10,000 PFU/mL, with a detection limit of 50 PFU/mL. From a series of proof-of-concept and clinical experiments, the developed PAFI biosensing system provided robust signal production and enhancement, as well as an excellent selectivity and sensitivity for influenza viruses. This nanoparticle-based technique could be potentially developed as an efficient detection platform for the influenza virus.
authors.affiliation: Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan; Department of Mechanical and Manufacturing Engineering, University of Calgary, Calgary, Canada, T2N 1N4.
authors.forename: Jaewook
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan; Department of Nano Fusion and Cogno-Mechatronics Engineering, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Syed Rahin
authors.initials: SR
authors.lastname: Ahmed
authors.affiliation: Department of Nano Fusion and Cogno-Mechatronics Engineering, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Sangjin
authors.initials: S
authors.lastname: Oh
authors.affiliation: Department of Nano Fusion and Cogno-Mechatronics Engineering, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Jeonghyo
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Infectious Diseases, Hamamatsu University School of Medicine, 1-20-1 Higashi-ku, Handa-yama, Hamamatsu 431-3192, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Department of Mechanical and Manufacturing Engineering, University of Calgary, Calgary, Canada, T2N 1N4.
authors.forename: Kaushik
authors.initials: K
authors.lastname: Parmar
authors.affiliation: Department of Mechanical and Manufacturing Engineering, University of Calgary, Calgary, Canada, T2N 1N4.
authors.forename: Simon S
authors.initials: SS
authors.lastname: Park
authors.affiliation: Department of Nano Fusion and Cogno-Mechatronics Engineering, Pusan National University, Busan 609-735, Republic of Korea. Electronic address: jaebeom@pusan.ac.kr.
authors.forename: Jaebeom
authors.initials: J
authors.lastname: Lee
authors.affiliation: Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan; Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: acypark@ipc.shizuoka.ac.jp.
authors.forename: Enoch Y
authors.initials: EY
authors.lastname: Park
mesh_terms: D000906:Antibodies / Q000737:chemistry; D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype / Q000302:isolation & purification* / Q000472:pathogenicity; D007251:Influenza, Human; D008163:Luminescent Measurements; D053768:Metal Nanoparticles / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry; D045663:Quantum Dots
chemical_list: D000906:Antibodies; D037742:Nanotubes, Carbon; D006046:Gold
keywords: CdTe quantum dot; Gold nanoparticle-decorated carbon nanotube; Influenza virus detection platform; Plasmon-assisted fluoro-immunoassay; Plasmonic resonance energy transfer