title: Fluorescence, aggregation properties and FT-IR microspectroscopy of elastin and collagen fibers.
abstract: Histological and histochemical observations support the hypothesis that collagen fibers can link to elastic fibers. However, the resulting organization of elastin and collagen type complexes and differences between these materials in terms of macromolecular orientation and frequencies of their chemical vibrational groups have not yet been solved. This study aimed to investigate the macromolecular organization of pure elastin, collagen type I and elastin-collagen complexes using polarized light DIC-microscopy. Additionally, differences and similarities between pure elastin and collagen bundles (CB) were investigated by Fourier transform-infrared (FT-IR) microspectroscopy. Although elastin exhibited a faint birefringence, the elastin-collagen complex aggregates formed in solution exhibited a deep birefringence and formation of an ordered-supramolecular complex typical of collagen chiral structure. The FT-IR study revealed elastin and CB peptide NH groups involved in different types of H-bonding. More energy is absorbed in the vibrational transitions corresponding to CH, CH2 and CH3 groups (probably associated with the hydrophobicity demonstrated by 8-anilino-1-naphtalene sulfonic acid sodium salt [ANS] fluorescence), and to νCN, δNH and ωCH2 groups of elastin compared to CB. It is assumed that the α-helix contribution to the pure elastin amide I profile is 46.8%, whereas that of the B-sheet is 20% and that unordered structures contribute to the remaining percentage. An FT-IR profile library reveals that the elastin signature within the 1360-1189cm(-1) spectral range resembles that of Conex-Toray aramid fibers.
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, University of Campinas (Unicamp), Rua Monteiro Lobato 255, CEP 013083-862 Campinas, SP, Brazil. Electronic address: camposvi@unicamp.br.
authors.forename: Benedicto de Campos
authors.initials: Bde C
authors.lastname: Vidal
mesh_terms: D000818:Animals; D016880:Anisotropy; D002417:Cattle; D003094:Collagen / Q000378:metabolism*; D024042:Collagen Type I / Q000378:metabolism; D004549:Elastin / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D024042:Collagen Type I; D003094:Collagen; D004549:Elastin
keywords: Birefringence; Collagen bundles; Collagen type I; Elastin; FT-IR; Hydrophobicity