title: A depth estimation method based on geometric transformation for stereo light microscope.
abstract: Stereo light microscopes (SLM) with narrow vision and shallow depth of field are widely used in micro-domain research. In this paper, we propose a depth estimation method of micro objects based on geometric transformation. By analyzing the optical imaging geometry, the definition of geometric transformation distance is given and the depth-distance relation express is obtained. The parameters of geometric transformation and express are calibrated with calibration board images captured in aid of precise motorized stage. The depth of micro object can be estimated by calculating the geometric transformation distance. The proposed depth-distance relation express is verified using an experiment in which the depth map of an Olanzapine tablet surface is reconstructed.
authors.affiliation: Faculty of Information Science and Engineering, Ningbo University, Ningbo 315211, China School of Information Science and Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Fan
authors.affiliation: Faculty of Information Science and Engineering, Ningbo University, Ningbo 315211, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yu
authors.affiliation: Faculty of Information Science and Engineering, Ningbo University, Ningbo 315211, China School of Information Science and Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China.
authors.forename: Yigang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Faculty of Information Science and Engineering, Ningbo University, Ningbo 315211, China.
authors.forename: Gangyi
authors.initials: G
authors.lastname: Jiang
mesh_terms: D000465:Algorithms; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
keywords: Stereo light microscope; camera calibration; depth reconstruction; geometric transformation distance