title: Color-coded perfusion analysis of CEUS for pre-interventional diagnosis of microvascularisation in cases of vascular malformations.
abstract: OBJECTIVE
  Aim of our pilot study was the application of a contrast-enhanced color-coded ultrasound perfusion analysis in patients with vascular malformations to quantify microcirculatory alterations.
  
  
  METHODS
  28 patients (16 female, 12 male, mean age 24.9 years) with high flow (n = 6) or slow-flow (n = 22) malformations were analyzed before intervention. An experienced examiner performed a color-coded Doppler sonography (CCDS) and a Power Doppler as well as a contrast-enhanced ultrasound after intravenous bolus injection of 1 - 2.4 ml of a second-generation ultrasound contrast medium (SonoVue®, Bracco, Milan). The contrast-enhanced examination was documented as a cine sequence over 60 s. The quantitative analysis based on color-coded contrast-enhanced ultrasound (CEUS) images included percentage peak enhancement (%peak), time to peak (TTP), area under the curve (AUC), and mean transit time (MTT).
  
  
  RESULTS
  No side effects occurred after intravenous contrast injection. The mean %peak in arteriovenous malformations was almost twice as high as in slow-flow-malformations. The area under the curve was 4 times higher in arteriovenous malformations compared to the mean value of other malformations. The mean transit time was 1.4 times higher in high-flow-malformations compared to slow-flow-malformations. There was no difference regarding the time to peak between the different malformation types. The comparison between all vascular malformation and surrounding tissue showed statistically significant differences for all analyzed data (%peak, TTP, AUC, MTT; p < 0.01). High-flow and slow-flow vascular malformations had statistically significant differences in %peak (p < 0.01), AUC analysis (p < 0.01), and MTT (p < 0.05).
  
  
  CONCLUSIONS
  Color-coded perfusion analysis of CEUS seems to be a promising technique for the dynamic assessment of microvasculature in vascular malformations.
authors.affiliation: Department of Radiology, University Hospital Regensburg, Regensburg, Germany.
authors.forename: V I
authors.initials: VI
authors.lastname: Teusch
authors.affiliation: Department of Radiology, University Hospital Regensburg, Regensburg, Germany.
authors.forename: W A
authors.initials: WA
authors.lastname: Wohlgemuth
authors.affiliation: Fürst Medical Laboratory, Oslo, Norway.
authors.forename: A P
authors.initials: AP
authors.lastname: Piehler
authors.affiliation: Department of Radiology, University Hospital Regensburg, Regensburg, Germany.
authors.forename: E M
authors.initials: EM
authors.lastname: Jung
mesh_terms: D000293:Adolescent; D000328:Adult; D019540:Area Under Curve; D001165:Arteriovenous Malformations / Q000000981:diagnostic imaging* / Q000503:physiopathology*; D002648:Child; D002675:Child, Preschool; D003116:Color; D003287:Contrast Media / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D008833:Microcirculation; D008875:Middle Aged; D010477:Perfusion / Q000379:methods*; D010865:Pilot Projects; D014463:Ultrasonography; D018615:Ultrasonography, Doppler, Color; D055815:Young Adult
chemical_list: D003287:Contrast Media
keywords: Contrast-enhanced ultrasound (CEUS); color-coded perfusion analysis; microcirculation; vascular malformations