title: Application of MALDI-TOF mass spectrometry in clinical diagnostic microbiology.
abstract: Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently emerged as a powerful technique for identification of microorganisms, changing the workflow of well-established laboratories so that its impact on microbiological diagnostics has been unparalleled. In comparison with conventional identification methods that rely on biochemical tests and require long incubation procedures, MALDI-TOF MS has the advantage of identifying bacteria and fungi directly from colonies grown on culture plates in a few minutes and with simple procedures. Numerous studies on different systems available demonstrate the reliability and accuracy of the method, and new frontiers have been explored besides microbial species level identification, such as direct identification of pathogens from positive blood cultures, subtyping, and drug susceptibility detection.
authors.affiliation: Catholic University of the Sacred Hearth, Rome, Italy. elena.decarolis@rm.unicatt.it.
authors.forename: Elena
authors.initials: E
authors.lastname: De Carolis
authors.forename: Antonietta
authors.initials: A
authors.lastname: Vella
authors.forename: Luisa
authors.initials: L
authors.lastname: Vaccaro
authors.forename: Riccardo
authors.initials: R
authors.lastname: Torelli
authors.forename: Teresa
authors.initials: T
authors.lastname: Spanu
authors.forename: Barbara
authors.initials: B
authors.lastname: Fiori
authors.forename: Brunella
authors.initials: B
authors.lastname: Posteraro
authors.forename: Maurizio
authors.initials: M
authors.lastname: Sanguinetti
mesh_terms: D001419:Bacteria / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D005658:Fungi / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*