title: Bacteria of the candidate phylum TM7 are prevalent in acidophilic nitrifying sequencing-batch reactors.
abstract: Laboratory-scale acidophilic nitrifying sequencing-batch reactors (ANSBRs) were constructed by seeding with sewage-activated sludge and cultivating with ammonium-containing acidic mineral medium (pH 4.0) with or without a trace amount of yeast extract. In every batch cycle, the pH varied between 2.7 and 4.0, and ammonium was completely converted to nitrate. Attempts to detect nitrifying functional genes in the fully acclimated ANSBRs by PCR with previously designed primers mostly gave negative results. 16S rRNA gene-targeted PCR and a subsequent denaturating gradient gel electrophoresis analysis revealed that a marked change occurred in the bacterial community during the overall period of operation, in which members of the candidate phylum TM7 and the class Gammaproteobacteria became predominant at the fully acclimated stage. This result was fully supported by a 16S rRNA gene clone library analysis, as the major phylogenetic groups of clones detected (>5% of the total) were TM7 (33%), Gammaproteobacteria (37%), Actinobacteria (10%), and Alphaproteobacteria (8%). Fluorescence in situ hybridization with specific probes also demonstrated the prevalence of TM7 bacteria and Gammaproteobacteria. These results suggest that previously unknown nitrifying microorganisms may play a major role in ANSBRs; however, the ecophysiological significance of the TM7 bacteria predominating in this process remains unclear.
authors.affiliation: Department of Environmental and Life Sciences, Toyohashi University of Technology.
authors.forename: Akiko
authors.initials: A
authors.lastname: Hanada
authors.forename: Takashi
authors.initials: T
authors.lastname: Kurogi
authors.forename: Nguyen Minh
authors.initials: NM
authors.lastname: Giang
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamada
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kamimoto
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kiso
authors.forename: Akira
authors.initials: A
authors.lastname: Hiraishi
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism; D001419:Bacteria / Q000145:classification* / Q000235:genetics; D019149:Bioreactors / Q000382:microbiology*; D058448:Biota; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D058465:Nitrification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
chemical_list: D064751:Ammonium Compounds; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012722:Sewage