title: Inhibitory effect of insulin-like growth factor-binding protein-7 (IGFBP7) on in vitro angiogenesis of vascular endothelial cells in the rat corpus luteum.
abstract: Angiogenesis in the developing corpus luteum (CL) is a prerequisite for establishment and maintenance of an early pregnancy. To explore the physiological significance of insulin-like growth factor-binding protein-7 (IGFBP7) in the developing CL, the effects of IGFBP7 on vascular endothelial growth factor (VEGFA)- and luteinizing hormone (LH)-induced in vitro tube formation were tested using isolated luteal microvascular endothelial cells (LECs). Capillary-like tube formation of LECs and their proliferation were stimulated by both VEGFA and LH. IGFBP7 treatment suppressed VEGFA- or LH-induced tube formation. The proliferation and migration of LECs, and phosphorylation of mitogen-activated protein kinase kinase and extracellular signal-regulated kinase 1/2 were inhibited by IGFBP7. Furthermore, IGFBP7 attenuated VEGFA-enhanced cyclooxygenase (COX)-2 mRNA expression and prostaglandin E2 secretion. These findings suggest the possibility that luteal IGFBP7 secretion may suppress the stimulatory effect of VEGFA on angiogenesis in the early CL.
authors.affiliation: Department of Endocrine and Neural Pharmacology, Tokyo University of Pharmacy and Life Sciences, Tokyo 192-0392, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Tamura
authors.forename: Mikihiro
authors.initials: M
authors.lastname: Yoshie
authors.forename: Keisuke
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Eiichi
authors.initials: E
authors.lastname: Tachikawa
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002465:Cell Movement; D049109:Cell Proliferation; D003338:Corpus Luteum / Q000166:cytology*; D042783:Endothelial Cells / Q000187:drug effects* / Q000502:physiology*; D016228:Endothelial Growth Factors / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D018969:Insulin-Like Growth Factor Binding Proteins / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000494:pharmacology; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011247:Pregnancy; D051381:Rats; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology
chemical_list: D016228:Endothelial Growth Factors; D018969:Insulin-Like Growth Factor Binding Proteins; D042461:Vascular Endothelial Growth Factor A; C102529:insulin-like growth factor binding protein-related protein 1; D007986:Luteinizing Hormone