title: Bioactive hemicelluloses alkali-extracted from Fallopia sachalinensis leaves.
abstract: Fallopia sachalinensis, regarded as an invasive plant in Europe and designated for disposal, is traditionally used in Japan and China as herbal medicine. Attempted for valorization of the leaves, this paper reports on two protein-free polysaccharide fractions, a neutral (FS-5A) and an acidic (FS-5B) one, obtained via alkali extraction and consecutive purification. Both fractions were characterized by chemical, molecular, structural and bioactive properties. FTIR and 1D/2D NMR analyses revealed that FS-5A consisted of a fucogalactoxyloglucan, whereas, glucuronoxylan was the major hemicellulose in FS-5B accompanied with low proportions of fucosylated xyloglucan and pectic RG-I. Both hemicellulose fractions exhibited significant immunostimulating activity in the complement-fixation test and the latter had noticeable DPPH radical-scavenging ability. The results completed information about neutral and acidic bioactive polysaccharide components present in the leaves of F. sachalinensis.
authors.affiliation: Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38 Bratislava, Slovak Republic. Electronic address: chemzuko@savba.sk.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Košťálová
authors.affiliation: Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38 Bratislava, Slovak Republic.
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Hromádková
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, University of Oslo, Oslo, Norway.
authors.forename: Berit Smestad
authors.initials: BS
authors.lastname: Paulsen
authors.affiliation: Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38 Bratislava, Slovak Republic.
authors.forename: Anna
authors.initials: A
authors.lastname: Ebringerová
mesh_terms: D000468:Alkalies / Q000737:chemistry*; D000818:Animals; D003165:Complement System Proteins / Q000378:metabolism; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D018515:Plant Leaves / Q000737:chemistry*; D019664:Polygonaceae / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012756:Sheep
chemical_list: D000468:Alkalies; D016166:Free Radical Scavengers; D011134:Polysaccharides; C007916:hemicellulose; D003165:Complement System Proteins
keywords: Bioactivity; Fallopia sachalinensis; Glucuronoxylan; NMR; Xyloglucan