title: miR-148b functions as a tumor suppressor in non-small cell lung cancer by targeting carcinoembryonic antigen (CEA).
abstract: Aberrant expression of miR-148b has been found in several types of cancer, but its expression and potential biologic role in non-small cell lung cancer (NSCLC) are still largely unknown. Here, we found that miR-148b was commonly under-expressed in human non-small celllung cancer (NSCLC) specimens and cell lines. The overexpression of miR-148b dramatically suppressed NSCLC cell proliferation and migration. Furthermore, miR-148b could regulate carcinoembryonic antigen (CEA) expression by luciferase reporter assay. On the other hand, CEA was widely up-regulated in NSCLC specimens, and its mRNA levels were inversely correlated with miR-148b expression. These suggest that CEA expression may be regulated by miR-148b. Collectively, our findings indicate miR-148b is low expression in NSCLC cells, which results in CEA overexpression and disease progression in NSCLC patients.
authors.affiliation: Department of Oncology, Guangzhou First Municipal People's Hospital Affiliated to Guangzhou Medical College 1 Panfu Road, Guangzhou, P. R. China.
authors.forename: Guo-Long
authors.initials: GL
authors.lastname: Liu
authors.affiliation: Department of Oncology, Guangzhou First Municipal People's Hospital Affiliated to Guangzhou Medical College 1 Panfu Road, Guangzhou, P. R. China.
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangdong Higher Education Institutes, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University 107 Yanjiang West Road, Guangzhou, P. R. China ; Research Center of Medicine, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University 107 Yanjiang West Road, Guangzhou, P. R. China.
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Lv
authors.affiliation: Department of Pathology, Guangzhou First Municipal People's Hospital Affiliated to Guangzhou Medical College 1 Panfu Road, Guangzhou, P. R. China.
authors.forename: Xiao-Pai
authors.initials: XP
authors.lastname: Wang
authors.affiliation: Department of Oncology, Guangzhou First Municipal People's Hospital Affiliated to Guangzhou Medical College 1 Panfu Road, Guangzhou, P. R. China.
authors.forename: Xi-Sheng
authors.initials: XS
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center Guangzhou 510060, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Sang
keywords: CEA; NSCLC; miR-148b; migration; tumor suppressors