title: Flavonoids and phenolic acids from Labisia pumila (Kacip Fatimah).
abstract: Both total phenolic content (TPC) and total flavonoid content (TFC) of Labisia pumila extracts were determined spectrophotometrically. L. pumila leaves extracted in 60% methanol (MeOH) were fractionated on C18 cartridge and the antioxidant property of each fraction was determined by measuring free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity. The 40% MeOH fraction exhibited the highest scavenging activity. Nine flavonols (quercetin, myricetin and kaempferol), two flavanols (catechin and epigallocatechin) and nine phenolic acids were identified from this active fraction by UPLC-ESI-MS/MS, and confirmed by comparison with the mass spectra of standard aglycones, theoretical fragments generated from MS Fragmenter software, and literature values.
authors.affiliation: Metabolites Profiling Laboratory, Chemical Engineering Pilot Plant, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia. Electronic address: chualeesuan@yahoo.com.
authors.forename: Lee Suan
authors.initials: LS
authors.lastname: Chua
authors.affiliation: Metabolites Profiling Laboratory, Chemical Engineering Pilot Plant, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia.
authors.forename: Norliza Abdul
authors.initials: NA
authors.lastname: Latiff
authors.affiliation: Metabolites Profiling Laboratory, Chemical Engineering Pilot Plant, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia.
authors.forename: Sze Yean
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Bioprocess Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia.
authors.forename: Chew Tin
authors.initials: CT
authors.lastname: Lee
authors.affiliation: Metabolites Profiling Laboratory, Chemical Engineering Pilot Plant, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia.
authors.forename: Mohamad Roji
authors.initials: MR
authors.lastname: Sarmidi
authors.affiliation: Metabolites Profiling Laboratory, Chemical Engineering Pilot Plant, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia.
authors.forename: Ramlan Abdul
authors.initials: RA
authors.lastname: Aziz
keywords: Flavanols; Flavonols; Labisia pumila; Phenolic acids; UPLC–ESI-MS/MS