title: Pseudanoplocephala crawfordi is a member of genus Hymenolepis based on phylogenetic analysis using ribosomal and mitochondrial DNA sequences.
abstract: Pseudanoplocephala crawfordi is one of the important zoonotic cestodes causing economic significance and public health concern. In the present study, the phylogenetic position of P. crawfordi isolated from pigs was re-inferred using molecular markers of internal transcribed spacer ribosomal DNA (ITS rDNA) and partial NADH dehydrogenase subunit 1 (pnad1) mitochondrial DNA. The lengths of ITS1, ITS2 rDNA and pnad1 were 757 bp, 628 bp and 458 bp, respectively. Sequence differences in the ITS1, ITS2 rDNA and pnad1 between P. crawfordi and Hymenolepis species were smaller than that between cestodes within genus Hymenolepis. Phylogenetic analyses based on three gene fragments showed that P. crawfordi was grouped into cluster of Hymenolepis species. These results suggested that P. crawfordi would be one member of genus Hymenolepis but not in a new genus Pseudanoplocephala.
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi Province , People's Republic of China and.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Jia
authors.affiliation: b Laboratory of Animal Quarantine , College of Animal Science and Technology, Henan University of Science and Technology , Luoyang , Henan Province , People's Republic of China.
authors.forename: Wen-Chao
authors.initials: WC
authors.lastname: Yan
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi Province , People's Republic of China and.
authors.forename: Shuai-Zhi
authors.initials: SZ
authors.lastname: Du
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi Province , People's Republic of China and.
authors.forename: Jun-Ke
authors.initials: JK
authors.lastname: Song
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi Province , People's Republic of China and.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhao
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi Province , People's Republic of China and.
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Zhao
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi Province , People's Republic of China and.
authors.forename: Wen-Yu
authors.initials: WY
authors.lastname: Cheng
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi Province , People's Republic of China and.
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D005843:Geography; D006926:Hymenolepis / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D013552:Swine / Q000469:parasitology
chemical_list: D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal
keywords: ITS rDNA; Pseudanoplocephala crawfordi; mitochondrial DNA; phylogenetic analyses