title: Qualitative and quantitative analysis of curcuminoids in herbal medicines derived from Curcuma species.
abstract: A validated and sensitive HPLC-UV-MS method was developed for qualitative and quantitative analysis of curcuminoids in eight herbal medicines derived from four Curcuma species. The samples were separated on a YMC ODS-A C18 column with a gradient elution of acetonitrile and 0.1% formic acid. Curcumin, demethoxycurcumin and bisdemethoxycurcumin showed good linearity (r>0.9998) in the concentration ranges of 4.88-625, 4.29-550 and 3.98-510μg/mL, respectively. The results suggested that the contents of three major curcuminoids in different herbal medicines varied significantly. Curcuminoids were only detected in Jianghuang, HuangsiYujin, and PengEzhu. Amongst them, Jianghuang contained the highest amounts of curcuminoids (40.36mg/g), which were almost 20 times higher than HuangsiYujin (1.94mg/g) and 400 times higher than PengEzhu (0.098mg/g). Furthermore, amongst the Jianghuang samples collected from different areas, samples from Sichuan Province contained remarkably higher amounts of curcuminoids (22.21-40.36mg/g) than other cultivation regions.
authors.affiliation: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, PR China. Electronic address: liruielite@gmail.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, PR China. Electronic address: damaocheng@gmail.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Xiang
authors.affiliation: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, PR China. Electronic address: yemin@bjmu.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Ye
authors.affiliation: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, PR China. Electronic address: honghaier888@sina.com.
authors.forename: Hui-Fang
authors.initials: HF
authors.lastname: Li
authors.affiliation: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, PR China. Electronic address: zx10china@126.com.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, PR China. Electronic address: gda5958@163.com.
authors.forename: De-An
authors.initials: DA
authors.lastname: Guo
keywords: Curcuma spp.; Curcuminoids; HPLC–UV–MS; Herbal medicines