title: Dysregulated microRNA expression in adenocarcinoma of the uterine cervix: clinical impact of miR-363-3p.
abstract: OBJECTIVE
  Adenocarcinoma (ACA) of the uterine cervix is increasing in incidence and currently accounts for approximately 20% of all cervical malignancies. MicroRNAs (miRNAs) have been investigated as potential biomarkers of cervical cancer; however, their role in ACA remains unknown. Here, we characterized miRNA expression profiles and investigated miRNAs as diagnostic and prognostic factors in ACA.
  
  
  METHODS
  Evaluation of genome-wide miRNA expression profiles in ACA by microarray led to the identification of ten candidate miRNAs, whose expression patterns were validated by qRT-PCR in 45 ACA, 10 normal control, and 15 squamous cell carcinoma samples. The association between miRNA expression and prognosis was analyzed in patients with ACA.
  
  
  RESULTS
  Microarray analysis identified 86 miRNAs that were dysregulated more than 2.0-fold (p<0.05) in ACA relative to normal tissues of the uterine cervix. Five most over- and underexpressed miRNAs were selected respectively and their expression patterns were confirmed in the validation set. MiR-135b, miR-192, and miR194 were overexpressed in ACA, and miR-363-3p, miR-195 and miR-199b were significantly associated with conventional prognostic factors. Overexpression of miR-363-3p by more than 2.5-fold relative to the normal control was a strong predictor of favorable prognosis (hazard ratio, 0.1; 95% confidence interval, 0.009-0.779) after adjusting for confounders.
  
  
  CONCLUSIONS
  MiR-135b, miR-192, and miR-194 are altered in uterine cervical ACA, and miR-363-3p is an independent favorable prognostic factor in ACA. These miRNAs could be of value as biomarkers for the diagnosis and prognosis of ACA.
authors.affiliation: Department of Gynecological Oncology, CHA University, Sungnam, Republic of Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Park
authors.affiliation: Institute for Clinical Research, CHA University, Sungnam, Republic of Korea.
authors.forename: Mi-jung
authors.initials: MJ
authors.lastname: Lee
authors.affiliation: Institute for Clinical Research, CHA University, Sungnam, Republic of Korea.
authors.forename: Ju-yeon
authors.initials: JY
authors.lastname: Jeong
authors.affiliation: Department of Gynecological Oncology, CHA University, Sungnam, Republic of Korea.
authors.forename: Min Chul
authors.initials: MC
authors.lastname: Choi
authors.affiliation: Department of Gynecological Oncology, CHA University, Sungnam, Republic of Korea.
authors.forename: Sang Geun
authors.initials: SG
authors.lastname: Jung
authors.affiliation: Department of Gynecological Oncology, CHA University, Sungnam, Republic of Korea.
authors.forename: Won Duk
authors.initials: WD
authors.lastname: Joo
authors.affiliation: Department of Gynecological Oncology, CHA University, Sungnam, Republic of Korea.
authors.forename: Chan
authors.initials: C
authors.lastname: Lee
authors.affiliation: Institute for Clinical Research, CHA University, Sungnam, Republic of Korea; Department of Pathology, CHA University, Sungnam, Republic of Korea.
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: An
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D005260:Female; D005838:Genotype; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D046228:Microarray Analysis; D008875:Middle Aged; D011379:Prognosis; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism
chemical_list: C574861:MIRN363 microRNA, human; D035683:MicroRNAs
keywords: Biomarker; Cervical adenocarcinoma; miR-363-3p; microRNA microarray