title: Improved biomass and lipid production in a mixotrophic culture of Chlorella sp. KR-1 with addition of coal-fired flue-gas.
abstract: Industrial CO2-rich flue-gases, owing to their eco-toxicity, have yet to be practically exploited for microalgal biomass and lipid production. In this study, various autotrophic and mixotrophic culture modes for an oleaginous microalga, Chlorella sp. KR-1 were compared for the use in actual coal-fired flue-gas. Among the mixotrophic conditions tested, the fed-batch feedings of glucose and the supply of air in dark cycles showed the highest biomass (561 mg/L d) and fatty-acid methyl-ester (168 mg/L d) productivities. This growth condition also resulted in the maximal population of microalgae and the minimal population and types of KR-1-associated-bacterial species as confirmed by particle-volume-distribution and denaturing-gradient-gel-electrophoresis (DGGE) analyses. Furthermore, microalgal lipid produced was assessed, based on its fatty acid profile, to meet key biodiesel standards such as saponification, iodine, and cetane numbers.
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research (KIER), Daejeon 305-343, Republic of Korea.
authors.forename: Ramasamy
authors.initials: R
authors.lastname: Praveenkumar
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research (KIER), Daejeon 305-343, Republic of Korea.
authors.forename: Bohwa
authors.initials: B
authors.lastname: Kim
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research (KIER), Daejeon 305-343, Republic of Korea.
authors.forename: Eunji
authors.initials: E
authors.lastname: Choi
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research (KIER), Daejeon 305-343, Republic of Korea.
authors.forename: Kyubock
authors.initials: K
authors.lastname: Lee
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research (KIER), Daejeon 305-343, Republic of Korea.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Park
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research (KIER), Daejeon 305-343, Republic of Korea.
authors.forename: Jin-Suk
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of BioNano Technology, Gachon University, Seongnam-si, Gyeonggi-do 461-701, Republic of Korea.
authors.forename: Young-Chul
authors.initials: YC
authors.lastname: Lee
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research (KIER), Daejeon 305-343, Republic of Korea. Electronic address: ykoh@kier.re.kr.
authors.forename: You-Kwan
authors.initials: YK
authors.lastname: Oh
mesh_terms: D000704:Analysis of Variance; D056804:Biofuels; D019149:Bioreactors / Q000382:microbiology*; D002245:Carbon Dioxide / Q000494:pharmacology*; D002708:Chlorella / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D003031:Coal / Q000032:analysis; D019295:Computational Biology; D058645:Denaturing Gradient Gel Electrophoresis; D008055:Lipids / Q000096:biosynthesis*; D013045:Species Specificity
chemical_list: D056804:Biofuels; D003031:Coal; D008055:Lipids; D002245:Carbon Dioxide
keywords: Chlorella sp.; Coal-fired flue-gas; Fed-batch; Lipid; Mixotrophic culture