title: Histopathological evaluation of tumor necrosis and volume after cyanogenic chemotherapy.
abstract: OBJECTIVE
  To determine the percentage of tumoral necrosis and volume after cyanogenic chemotherapy.
  
  
  METHODS
  Histopathological findings of 20 Swiss mice inoculated subcutaneously in the left abdominal wall with 0.05 ml of cell suspension containing 2.5 x 10(5) viable cells of the Ehrlich tumor were evaluated. The tumor response to cyanogenic chemotherapy was determined using a system that comprises two inhibition factors of tumor growth by calculating the percentage of necrosis in the tumor tissue and calculation of tumor volume in treated animals relative to that in control animals. The importance of this system has been validated by the correlation between tumor inhibition in the groups treated with the respective percentages of necrosis.
  
  
  RESULTS
  While the control group presented an average of 13.48 ± 14.71% necrosis and average tumor volume of 16.18 ± 10.94, the treated group had an average of 42.02 ± 11.58 and 6.8 ± 3.57, respectively. The tumor inhibition was significantly associated with treatment (p=0.0189). The analysis of necrosis percentage showed a significant prognostic importance (p=0.0001).
  
  
  CONCLUSIONS
  It is concluded that the effect of cyanogenic chemotherapy showed strong inhibitory action of tumor growth, as well as an increase in its area of necrosis.
authors.affiliation: Federal University of Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Rondon Tosta
authors.initials: RT
authors.lastname: Ramalho
authors.affiliation: Department of Surgery, UFMS, Campo Grande, MS, Brazil.
authors.forename: Ricardo Dutra
authors.initials: RD
authors.lastname: Aydos
authors.affiliation: Department of Surgery, UFMS, Campo Grande, MS, Brazil.
authors.forename: Iandara
authors.initials: I
authors.lastname: Schettert
authors.affiliation: UFMS, Campo Grande, MS, Brazil.
authors.forename: Pedro Carvalho
authors.initials: PC
authors.lastname: Cassino
mesh_terms: D034861:Abdominal Wall; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D009336:Necrosis / Q000188:drug therapy / Q000473:pathology; D009368:Neoplasm Transplantation / Q000379:methods; D009570:Nitriles / Q000009:adverse effects / Q000378:metabolism / Q000627:therapeutic use*; D011897:Random Allocation; D012016:Reference Values; D015203:Reproducibility of Results; D013466:Sulfurtransferases / Q000378:metabolism; D016896:Treatment Outcome; D047368:Tumor Burden / Q000187:drug effects*
chemical_list: D000970:Antineoplastic Agents; D009570:Nitriles; C011206:cyanogen; C010833:acetone cyanohydrin; D013466:Sulfurtransferases; C063506:sulfane sulfurtransferase