title: Lab-on-a-Chip hyphenation with mass spectrometry: strategies for bioanalytical applications.
abstract: The Lab-on-a-Chip concept aims at miniaturizing laboratory processes to enable automation and/or parallelization via microfluidic chips that are capable of handling minute sample volumes. Mass spectrometry is nowadays the detection method of choice, because of its selectivity, sensitivity and wide application range. We review the most interesting examples over the last two-and-a-half years where the two techniques were used for bioanalytical applications. Furthermore, we discuss the merits and limitations of such hyphenated systems. We inventorize the reported applications and approaches. We see an ongoing trend towards chip-based liquid chromatography-mass spectrometry usage and small volume analysis applications, particularly in the field of proteomics where bottom-up approaches profit from chip-based technologies and hyphenation with complex cell cultures.
authors.affiliation: Division of Analytical Biosciences, Leiden Academic Centre for Drug Research, Leiden University, the Netherlands; Netherlands Metabolomics Centre, Leiden, the Netherlands.
authors.forename: Amar
authors.initials: A
authors.lastname: Oedit
authors.affiliation: Division of Analytical Biosciences, Leiden Academic Centre for Drug Research, Leiden University, the Netherlands; Netherlands Metabolomics Centre, Leiden, the Netherlands; Mimetas BV, Leiden, the Netherlands.
authors.forename: Paul
authors.initials: P
authors.lastname: Vulto
authors.affiliation: Division of Analytical Biosciences, Leiden Academic Centre for Drug Research, Leiden University, the Netherlands; Netherlands Metabolomics Centre, Leiden, the Netherlands.
authors.forename: Rawi
authors.initials: R
authors.lastname: Ramautar
authors.affiliation: Division of Analytical Biosciences, Leiden Academic Centre for Drug Research, Leiden University, the Netherlands; Netherlands Metabolomics Centre, Leiden, the Netherlands. Electronic address: p.lindenburg@lacdr.leidenuniv.nl.
authors.forename: Petrus W
authors.initials: PW
authors.lastname: Lindenburg
authors.affiliation: Division of Analytical Biosciences, Leiden Academic Centre for Drug Research, Leiden University, the Netherlands; Netherlands Metabolomics Centre, Leiden, the Netherlands.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hankemeier
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D004583:Electrons; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*