title: miR-21 induces cell proliferation and suppresses the chemosensitivity in glioblastoma cells via downregulation of FOXO1.
abstract: miR-21 shares a potential oncogenic function. The overexpression of miR-21 was common in glioblastoma, which is the most common lethal primary intracranial tumor. The study aimed at miR-21 effect on Glioblastoma cell line A172 of proliferation, apoptosis, and chemosensitivity and its definite mechanism of target gene FOXO1. Effect and mechanism were evaluated by colony forming cell assay, annexin V-FITC/PI apoptosis assay, TUNEL apoptosis assay, luciferase-reporter activities assay, RNA interference, western-blot and Real-Time PCR. The statistics revealed miR-21 promoted A172 cell proliferation and suppressed the chemosensitivity, and also showed that miR-21 could bind with FOXO1 mRNA and prevent FOXO1 translation via its 3'UTR to regulate the function. These findings suggest that miR-21 plays an important role in cell proliferation and chemosensitivity by inhibiting FOXO1, and show much more significance for exploring miR-21 inhibitor in A172 therapy.
authors.affiliation: Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Guangzhou, China ; Department of Neurosurgery, Nanfang Hospital, Southern Medical University Guangzhou, China.
authors.forename: Bing-Xi
authors.initials: BX
authors.lastname: Lei
authors.affiliation: Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Guangzhou, China.
authors.forename: Zheng-Hao
authors.initials: ZH
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Guangzhou, China.
authors.forename: Zhong-Jun
authors.initials: ZJ
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Guangzhou, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Guangzhou, China.
authors.forename: Yue-Fei
authors.initials: YF
authors.lastname: Deng
keywords: A172; FOXO1; chemosensitivity; miR-21; proliferation