title: Identification and isolation of putative stem cells from the murine placenta.
abstract: OBJECTIVE
  The placenta of mid-gestation mice is a known rich source of hematopoietic stem cells. We hypothesized that it is also a source of other multipotent stem cells.
  
  
  METHODS
  We isolated fetal cells from the murine placenta across the second half of gestation and characterized their expression of surface antigens known to be associated with mesenchymal stem cells (MSCs) on a subset of hematopoietic lineage-negative cells. Using real-time reverse-transcriptase quantitative polymerase chain reaction, we also evaluated the expression of intracellular transcription factors (TFs) known to be associated with renal development and/or multipotent stem cells.
  
  
  RESULTS
  Cell phenotypes with surface marker and TF expression consistent with multipotent stem cells of a mesenchymal lineage as well as renal cell progenitors were found in the placenta. The expression of MSC and renal progenitor surface markers varied throughout gestation, but was highest on E12-15 where such cells represented a small but significant percentage of the population. Of the studied TFs, 10 of 11 renal TFs were found at moderate to high levels, and all stem cell TFs were found.
  
  
  CONCLUSIONS
  The mid-gestation murine placenta may serve as a source of multipotent stem cells and also contains cells which may be renal cell progenitors.
authors.forename: Christine L
authors.initials: CL
authors.lastname: Proudfit
authors.forename: Michael K
authors.initials: MK
authors.lastname: Chan
authors.forename: Ross S
authors.initials: RS
authors.lastname: Basch
authors.forename: Bruce K
authors.initials: BK
authors.lastname: Young
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D051379:Mice; D039902:Multipotent Stem Cells / Q000378:metabolism; D010920:Placenta / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; D014157:Transcription Factors