title: Production of good manufacturing practice-grade human umbilical cord blood-derived mesenchymal stem cells for therapeutic use.
abstract: Human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) are multipotent stem cells that can be differentiated into several specific cell types such as adipocytes, osteoblasts, and chondroblasts. They also were demonstrated to trans-differentiate into other cell lineages such as muscle cells and neurons. Thus, they are considered a promising stem cell source for therapeutic use. Here, we describe a method for production of good manufacturing practice-grade human UCB-MSCs for therapeutic use. The obtained UCB-MSCs are free of allogenous or xenogenous proteins. In addition, these MSCs could maintain the MSC phenotype in long-term culture.
authors.affiliation: Laboratory of Stem Cell Research and Application, University of Science, Vietnam National University, Ho Chi Minh City, Vietnam, pvphuc@hcmuns.edu.vn.
authors.forename: Phuc
authors.initials: P
authors.lastname: Van Pham
authors.forename: Ngoc Kim
authors.initials: NK
authors.lastname: Phan
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods* / Q000592:standards*; D002469:Cell Separation / Q000379:methods / Q000592:standards; D064987:Cell- and Tissue-Based Therapy / Q000379:methods / Q000592:standards; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism