title: Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil.
abstract: Milk is widely consumed in Brazil and can be the vehicle of agent transmission. In this study, was evaluated the occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk consumed in the northwestern region of Paraná, Brazil. Fifty-two milk samples (20 pasteurized and 32 raw) from dairy farms near the municipality of Maringa, Parana State, Brazil were collected. Milk samples were decontaminated using 5% oxalic acid method and cultured on Lowenstein-Jensen and Stonebrink media at 35 °C and 30 °C, with and without 5-10% CO2. Mycobacteria isolates were identified by morphological features, PCR-Restriction Fragment Length Polymorphism Analysis (PCR-PRA) and Mycolic acids analysis. Thirteen (25%) raw and 2 (4%) pasteurized milk samples were positive for acid fast bacilli growth. Nine different species of NTM were isolated (M. nonchromogenicum, M. peregrinum, M. smegmatis, M. neoaurum, M. fortuitum, M. chelonae, M. flavescens, M. kansasii and M. scrofulaceum). M. bovis was not detected. Raw and pasteurized milk may be considered one source for NTM human infection. The paper reinforces the need for intensification of measures in order to avoid the milk contamination and consequently prevent diseases in the south of Brazil.
authors.affiliation: Departamento de Análises Clínicas e Biomedicina Universidade Estadual de Maringá MaringáPR Brazil Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá, Maringá, PR, Brazil.
authors.forename: Sônia Aparecida
authors.initials: SA
authors.lastname: Sgarioni
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas Universidade de São Paulo São PauloSP Brazil Departamento de Análises Clínicas e Toxicológicas, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Rosario Dominguez Crespo
authors.initials: RD
authors.lastname: Hirata
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas Universidade de São Paulo São PauloSP Brazil Departamento de Análises Clínicas e Toxicológicas, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Mario Hiroyuki
authors.initials: MH
authors.lastname: Hirata
authors.affiliation: Faculdade de Ciências Farmacêutica da Universidade Estadual Paulista AraraquaraSP Brazil Faculdade de Ciências Farmacêutica da Universidade Estadual Paulista, Araraquara, SP, Brazil.
authors.forename: Clarice Queico Fujimura
authors.initials: CQ
authors.lastname: Leite
authors.affiliation: Faculdade de Ciências Farmacêutica da Universidade Estadual Paulista AraraquaraSP Brazil Faculdade de Ciências Farmacêutica da Universidade Estadual Paulista, Araraquara, SP, Brazil.
authors.forename: Karina Andrade
authors.initials: KA
authors.lastname: de Prince
authors.affiliation: Instituto de Química da Universidade Estadual Paulista AraraquaraSP Brazil Instituto de Química da Universidade Estadual Paulista, Araraquara, SP, Brazil.
authors.forename: Sergio Roberto
authors.initials: SR
authors.lastname: de Andrade Leite
authors.affiliation: Secretaria de Saúde do Estado do Paraná MaringáPR Brazil Secretaria de Saúde do Estado do Paraná, Maringá, PR, Brazil.
authors.forename: Dirceu Vedovello
authors.initials: DV
authors.lastname: Filho
authors.affiliation: Departamento de Análises Clínicas e Biomedicina Universidade Estadual de Maringá MaringáPR Brazil Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá, Maringá, PR, Brazil.
authors.forename: Vera Lucia Dias
authors.initials: VL
authors.lastname: Siqueira
authors.affiliation: Departamento de Análises Clínicas e Biomedicina Universidade Estadual de Maringá MaringáPR Brazil Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá, Maringá, PR, Brazil.
authors.forename: Katiany Rizzieri
authors.initials: KR
authors.lastname: Caleffi-Ferracioli
authors.affiliation: Departamento de Análises Clínicas e Biomedicina Universidade Estadual de Maringá MaringáPR Brazil Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá, Maringá, PR, Brazil.
authors.forename: Rosilene Fressatti
authors.initials: RF
authors.lastname: Cardoso
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D001938:Brazil; D008892:Milk / Q000382:microbiology*; D009163:Mycobacterium bovis / Q000302:isolation & purification*; D009170:Nontuberculous Mycobacteria / Q000145:classification* / Q000302:isolation & purification*; D061352:Pasteurization; D066256:Raw Foods
keywords: Mycobacterium; PCR-PRA; milk; mycolic acids analysis; non-tuberculous mycobacteria