title: Protective efficacy of a single dose of baculovirus hemagglutinin-based vaccine in chickens and ducks against homologous and heterologous H5N1 virus infections.
abstract: Outbreaks of the highly pathogenic H5N1 virus in poultry and humans are ongoing. Vaccination is an efficient method for prevention of H5N1 infection. Using chickens and ducks, we assessed the efficacy of a vaccine comprising H5N1 hemagglutinin (HA) protein produced in a baculovirus expression system. The immunized chickens and ducks were protected against lethal infection by H5N1 in an antigen dose-dependent manner. Complete protection against homologous challenge and partial protection against heterologous challenge were achieved in chickens immunized with 5 μg HA protein and in ducks immunized with 10 μg HA protein. The IgG antibody subtype was mainly detected in the sera and tissues, including the lungs. The neuraminidase (NA) inhibition assay was negative in immunized chickens and ducks. Our results indicated that the expressed HA protein by baculovirus was immunogenic to both chickens and ducks, and the immunized chickens and ducks were protected from the lethal infections of highly pathogenic H5N1 influenza virus, though ducks required more HA protein than chickens to be protected. Also, baculovirus HA-vaccinated poultry can be differentiated from infected poultry by NA inhibition assay.
authors.affiliation: 1 Laboratory of Influenza Research, Chungnam National University , Daejeon, Republic of Korea.
authors.forename: Eun Hye
authors.initials: EH
authors.lastname: Park
authors.forename: Byung Min
authors.initials: BM
authors.lastname: Song
authors.forename: Jung
authors.initials: J
authors.lastname: Yum
authors.forename: Ji An
authors.initials: JA
authors.lastname: Kim
authors.forename: Seung Kyoo
authors.initials: SK
authors.lastname: Oh
authors.forename: Hyun Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Gil Jae
authors.initials: GJ
authors.lastname: Cho
authors.forename: Sang Heui
authors.initials: SH
authors.lastname: Seo
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002645:Chickens; D004337:Drug Carriers; D004372:Ducks; D005822:Genetic Vectors; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005585:Influenza in Birds / Q000517:prevention & control*; D008168:Lung / Q000276:immunology; D016019:Survival Analysis; D016896:Treatment Outcome; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D004337:Drug Carriers; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007074:Immunoglobulin G; D007252:Influenza Vaccines; D014614:Vaccines, Synthetic; C109963:hemagglutinin, avian influenza A virus