title: Sexual behavior attenuates the effects of chronic stress in body weight, testes, sexual accessory glands, and plasma testosterone in male rats.
abstract: The aim of this study was to evaluate whether continuous sexual behavior could attenuate the effects of chronic stress on spermatogenesis, sexual glands, plasma testosterone and corticosterone in sexually experienced male rats. Rats were exposed to stress by immersion in cold water (ICW) daily for 20 or 50 consecutive days. Plasma testosterone and corticosterone, masculine sexual behavior, as well as the number of offspring, the epithelial area of seminiferous, prostatic and seminal glands were assessed. In stressed males, body and testicular weights decreased, male sexual behavior was disrupted, and adrenal weights increased. In males stressed for 50 days, prostate and seminal glands had lower weights compared with controls. Prostate and seminal epithelial areas also decreased in these males. Seminiferous tubules in testes from rats stressed for 20 or 50 days showed several degenerative signs, such as vacuoles in the basal epithelium, with picnotic indicia; moderate to severe exfoliation of degenerative germinal cells in the tubule lumen was also observed. In males stressed for 50 days a significant decrease in seminiferous epithelial area was observed from stages I-VIII, regardless of copulation. The litters from females that copulated with males stressed for 50 days decreased significantly. Chronic stress caused increase in plasma levels of corticosterone, which were higher in males stressed for 20 days than in males stressed for 50 days. Testosterone decreased in stressed males and it was lower in males stressed for 50 days. In stressed males allowed to copulate, body and testicular weights were similar to controls. Adrenal, seminal glands, and prostate weights, as well as epithelial areas of males stressed for 50 days allowed to copulate were also similar to controls. Corticosterone was lower than in males stressed for 50 days, but still higher than in controls. Testosterone in males stressed for 50 days and allowed to copulate was higher than in stressed males not allowed to copulate and control males without copulation, but still lower than in control copulating males. These results show that chronic stress causes germ cell loss in testes and a decrease in prostate and seminal epithelium, possibly as a result of testosterone decrease, affecting fertility. Continuous copulation can attenuate the effects of stress on testosterone levels and on the epithelial area in male sexual glands, but not on the seminiferous epithelium after 50 days of stress.
authors.affiliation: Biología de la Reproducción, Universidad Autónoma Metropolitana-Iztapalapa, San Rafael Atlixco 186, Mexico City, CP 09340, Mexico. Electronic address: rems@xanum.uam.mx.
authors.forename: S
authors.initials: S
authors.lastname: Retana-Márquez
authors.affiliation: Laboratorio de Biología de la Reproducción, Instituto Nacional de Pediatría, Insurgentes Sur 3700-C, Insurgentes Cuicuilco, Mexico.
authors.forename: R M
authors.initials: RM
authors.lastname: Vigueras-Villaseñor
authors.affiliation: Biología de la Reproducción, Universidad Autónoma Metropolitana-Iztapalapa, San Rafael Atlixco 186, Mexico City, CP 09340, Mexico.
authors.forename: L
authors.initials: L
authors.lastname: Juárez-Rojas
authors.affiliation: Laboratorio de Biología de la Reproducción, Facultad de Ingeniería y Ciencias, Universidad Autónoma de Tamaulipas, Ciudad Victoria, Tamaulipas, Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Aragón-Martínez
authors.affiliation: Laboratorio de Biología de la Reproducción, Instituto Nacional de Pediatría, Insurgentes Sur 3700-C, Insurgentes Cuicuilco, Mexico.
authors.forename: G Reyes
authors.initials: GR
authors.lastname: Torres
mesh_terms: D000311:Adrenal Glands / Q000473:pathology*; D000818:Animals; D001835:Body Weight; D002908:Chronic Disease; D003307:Copulation / Q000502:physiology; D003345:Corticosterone / Q000097:blood; D005260:Female; D008297:Male; D009929:Organ Size; D011467:Prostate / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D012669:Seminal Vesicles / Q000473:pathology*; D012726:Sexual Behavior, Animal / Q000502:physiology*; D013091:Spermatogenesis / Q000502:physiology; D013315:Stress, Psychological / Q000097:blood / Q000473:pathology / Q000503:physiopathology*; D013737:Testis / Q000473:pathology*; D013739:Testosterone / Q000097:blood*
chemical_list: D013739:Testosterone; D003345:Corticosterone
keywords: Corticosterone; Fertility; Male sexual glands; Seminiferous epithelium; Sexual behavior; Spermatogenesis; Stress; Testosterone