title: Analysis of silencing in Saccharomyces cerevisiae.
abstract: Silencing assays have proven to be powerful tools not only for understanding how epigenetic processes function and defining the structural components of silent chromatin, but also for a useful readout for characterizing the functions of proteins involved in chromatin biology that influence epigenetic processes directly or indirectly. This chapter describes a collection of assays for monitoring silencing in Saccharomyces cerevisiae, including qualitative and quantitative methods as well as protocols that provide either indirect or direct measurements of the transcriptional state of loci regulated by silent chromatin.
authors.affiliation: Department of Biochemistry and Purdue University Center for Cancer Research, Purdue University, 175 S. University St., West Lafayette, IN, 47907, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Miller
authors.forename: Ann L
authors.initials: AL
authors.lastname: Kirchmaier
mesh_terms: D002843:Chromatin / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D020868:Gene Silencing; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
chemical_list: D002843:Chromatin; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae