title: Coordinated rearrangements of assimilatory and storage cell compartments in a nitrogen-starving symbiotic chlorophyte cultivated under high light.
abstract: A quantitative micromorphometric study of the cell compartment rearrangements was performed in a symbiotic chlorophyte Desmodesmus sp. 3Dp86E-1 grown on nitrogen (N) replete or N-free medium under 480 μmol PAR quanta m(-2) s(-1). The changes in the chloroplast, intraplastidial, and cytoplasmic inclusions induced by high light (HL) and N starvation were similar to those characteristic of free-living chlorophytes. The N-sufficient culture responded to HL by a transient swelling of the thylakoid lumen and a decline in photosynthetic efficiency followed by its recovery. In the N-starving cells, a more rapid expansion and thylakoid swelling occurred along with the irreversible decline in the photosynthetic efficiency. Differential induction of starch grains, oil bodies, and cell wall polysaccharides depending on the stress exposure and type was recorded. Tight relationships between the changes in the assimilatory and storage compartments in the stressed Desmodesmus sp. cells were revealed.
authors.affiliation: Department of Bioengineering Faculty of Biology, Moscow State University, GSP-1, 119234, Moscow, Russia.
authors.forename: Olga
authors.initials: O
authors.lastname: Gorelova
authors.forename: Olga
authors.initials: O
authors.lastname: Baulina
authors.forename: Alexei
authors.initials: A
authors.lastname: Solovchenko
authors.forename: Irina
authors.initials: I
authors.lastname: Selyakh
authors.forename: Olga
authors.initials: O
authors.lastname: Chivkunova
authors.forename: Larisa
authors.initials: L
authors.lastname: Semenova
authors.forename: Pavel
authors.initials: P
authors.lastname: Scherbakov
authors.forename: Olga
authors.initials: O
authors.lastname: Burakova
authors.forename: Elena
authors.initials: E
authors.lastname: Lobakova
mesh_terms: D002244:Carbon / Q000032:analysis / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism / Q000648:ultrastructure; D002734:Chlorophyll / Q000032:analysis / Q000378:metabolism; D000460:Chlorophyta / Q000502:physiology* / Q000528:radiation effects* / Q000648:ultrastructure; D008027:Light; D046529:Microscopy, Electron, Transmission; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism*; D010788:Photosynthesis / Q000502:physiology / Q000528:radiation effects; D013559:Symbiosis; D020524:Thylakoids / Q000378:metabolism
chemical_list: D002734:Chlorophyll; D002244:Carbon; D009584:Nitrogen