title: Association of ALOX5, LTA4H and LTC4S gene polymorphisms with ischemic stroke risk in a cohort of Chinese in east China.
abstract: BACKGROUND
  Genetic variations of the 5-lipoxygenase activating protein and leukotriene A4 hydrolase genes that confer an increased risk of ischemic stroke have implicated the family of leukotrienes as potential mediators of ischemic stroke. This study aimed to explore the association of ALOX5, LTA4H and LTC4S gene polymorphisms with ischemic stroke risk in a cohort of Chinese in east China.
  
  
  METHODS
  This case-control study consisted of 690 patients with ischemic stroke and 690 controls. Polymorphisms of ALOX5 rs2029253 A/G, LTA4H rs6538697 T/C, and LTC4S rs730012 A/C were genotyped by the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis. The multivariate logistic regression model was used to exclude the effects of conventional risk factors on ischemic stroke.
  
  
  RESULTS
  Carriers of C allele in rs730012 were more susceptible to ischemic stroke (OR: 1.37; 95%CI: 1.08-1.73; P=0.009). The rs2029253 GG genotype showed a risk-reducing effect on ischemic stroke (OR: 0.72; 95%CI: 0.55-0.93; P=0.013) while the rs6538697 CC genotype had an increased risk of ischemic stroke (OR: 1.77; 95%CI: 1.09-2.89; P=0.022). The rs730012 variant was not associated with ischemic stroke risk after adjusting confounding factors (P>0.05).
  
  
  CONCLUSIONS
  The present study suggested that gene polymorphisms in the leukotrienes pathway may exert influences, with independent genetic effects, on ischemic stroke susceptibility in a cohort of Chinese in east China.
authors.affiliation: Emergency Department, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Gan-Nan
authors.initials: GN
authors.lastname: Wang
authors.affiliation: Emergency Department, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Zhang
authors.affiliation: Emergency Department, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Wei-Juan
authors.initials: WJ
authors.lastname: Cao
authors.affiliation: Emergency Department, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Sun
authors.affiliation: Emergency Department, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Emergency Department, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Laboratory of Neurotoxicology, School of Public Health, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Xiao
keywords: Gene polymorphisms; Ischemic stroke; Leukotrienes; Risk factors