title: Lactococcus lactis IBB477 presenting adhesive and muco-adhesive properties as a candidate carrier strain for oral vaccination against influenza virus.
abstract: In the gastrointestinal tract (GIT), adhesion is a prerequisite for bacterial colonization. Lactococci can be used in functional food (probiotics) and health-related applications (mucosal vaccines, therapeutic drug delivery), both potentially involving adhesive properties. A candidate lactic acid bacterium for influenza antigen delivery through the GIT should display the ability to adhere. The present work probes the interactions between Lactococcus lactis and mucins using pig gastric mucin (PGM) as a model. Two strains were used for the optimization of the screening method for adhesion: L. lactis subsp. cremoris IBB477 persistent in the GIT of germ-free rats, and the low-adhering control strain MG1820. High adhesion to bare and mucin-coated polystyrene of IBB477 in comparison with MG1820 was observed. We searched for genetic determinants potentially involved in the adhesion/muco-adhesion of IBB477, identifying two such genes: prtP and a gene coding for a protein with MUB and MucBP domains. Based on its persistence in the GIT and adhesive properties, L. lactis IBB477 is a candidate carrier strain for expression of influenza haemagglutinin (HA) protein for induction of mucosal immune response.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Joanna M
authors.initials: JM
authors.lastname: Radziwill-Bienkowska
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland and Warsaw University of Technology, Warsaw, Poland.
authors.forename: Dominika
authors.initials: D
authors.lastname: Zochowska
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Bardowski
authors.affiliation: Université de Toulouse, INSA, UPS, INPT, LISBP, Toulouse, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, Toulouse, France and CNRS, UMR5504, Toulouse, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Mercier-Bonin
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kowalczyk
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D004337:Drug Carriers; D016503:Drug Delivery Systems; D041981:Gastrointestinal Tract / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D009980:Influenza A virus / Q000276:immunology; D007252:Influenza Vaccines / Q000008:administration & dosage*; D007251:Influenza, Human / Q000517:prevention & control; D013294:Lactococcus lactis / Q000378:metabolism; D009077:Mucins / Q000378:metabolism*; D013552:Swine; D014611:Vaccination / Q000379:methods*
chemical_list: D004337:Drug Carriers; D007252:Influenza Vaccines; D009077:Mucins