title: DNA fragmentation, caspase 3 and prostate-specific antigen genes expression induced by arsenic, cadmium, and chromium on nontumorigenic human prostate cells.
abstract: Prostate cancer is one of the most common cancers and the second cause of cancer-related deaths among men. Metals are recognized as chemical carcinogens where chronic exposures to such metals are implicated in the development of cancer, including prostate cancer. This in vitro study demonstrates the relative death sensitivity of prostatic (RWPE-1) cells to arsenic (As), cadmium (Cd), and chromium (Cr) as environmental pollutants through its apoptotic effects and the effect of these chemicals on prostate-specific antigen (PSA) gene expression as a marker for their carcinogecity. RWPE-1 cells were divided into three groups that were treated with As, Cd, and Cr in three replicates, at three different concentrations for each metal for 48 h. A control group consisted of untreated RWPE1 cells was used. Apoptosis was assessed using comet assay and caspase 3 gene expression; meanwhile, PSA gene expression was evaluated by semiqualitative real-time PCR (RT-PCR). One of the novel findings of this study is that arsenic and cadmium at low concentrations decreased apoptosis of RWPE-1 cells in a concentration-dependent manner while chromium induced significant concentration-dependent increase in apoptosis. Yet, at the highest concentrations, apoptosis was relatively more induced by all chemicals. Arsenic was the most chemical inhibiting apoptosis in RWPE-1 cells at low concentration. While at the moderate and highest concentrations, cadmium was the most inhibiting chemical of RWPE-1 cells' apoptosis. No distinct differences between treated and untreated cells for PSA gene expression were observed. It can be concluded that As and Cd, at low concentrations, can reduce apoptosis of prostatic cells in a concentration-dependent manner while chromium induced it; however, all metal salts used in this study did not induce PSA gene expression.
authors.affiliation: Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Hend M Abo
authors.initials: HM
authors.lastname: El-Atta
authors.forename: Amal A
authors.initials: AA
authors.lastname: El-Bakary
authors.forename: Afaf M
authors.initials: AM
authors.lastname: Attia
authors.forename: Ahmed
authors.initials: A
authors.lastname: Lotfy
authors.forename: Shery S
authors.initials: SS
authors.lastname: Khater
authors.forename: Ayman Z
authors.initials: AZ
authors.lastname: Elsamanoudy
authors.forename: Hussein Abdelaziz
authors.initials: HA
authors.lastname: Abdalla
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001151:Arsenic / Q000633:toxicity*; D002104:Cadmium / Q000633:toxicity*; D053148:Caspase 3 / Q000378:metabolism*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002857:Chromium / Q000633:toxicity*; D053938:DNA Fragmentation / Q000187:drug effects*; D006801:Humans; D008297:Male; D011467:Prostate / Q000166:cytology* / Q000378:metabolism*; D017430:Prostate-Specific Antigen / Q000378:metabolism*
chemical_list: D002104:Cadmium; D002857:Chromium; D017430:Prostate-Specific Antigen; D053148:Caspase 3; D001151:Arsenic