title: Chemically extracted acellular allogeneic nerve graft combined with ciliary neurotrophic factor promotes sciatic nerve repair.
abstract: A chemically extracted acellular allogeneic nerve graft can reduce postoperative immune rejection, similar to an autologous nerve graft, and can guide neural regeneration. However, it remains poorly understood whether a chemically extracted acellular allogeneic nerve graft combined with neurotrophic factors provides a good local environment for neural regeneration. This study investigated the repair of injured rat sciatic nerve using a chemically extracted acellular allogeneic nerve graft combined with ciliary neurotrophic factor. An autologous nerve anastomosis group and a chemical acellular allogeneic nerve bridging group were prepared as controls. At 8 weeks after repair, sciatic functional index, evoked potential amplitude of the soleus muscle, triceps wet weight recovery rate, total number of myelinated nerve fibers and myelin sheath thickness were measured. For these indices, values in the three groups showed the autologous nerve anastomosis group > chemically extracted acellular nerve graft + ciliary neurotrophic factor group > chemical acellular allogeneic nerve bridging group. These results suggest that chemically extracted acellular nerve grafts combined with ciliary neurotrophic factor can repair sciatic nerve defects, and that this repair is inferior to autologous nerve anastomosis, but superior to chemically extracted acellular allogeneic nerve bridging alone.
authors.affiliation: Institute of International Education, Zhengzhou University, Zhengzhou, Henan Province, China ; Institute of Clinical Anatomy, Southern Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Yanru
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute of Clinical Medicine, Zhengzhou University, Zhengzhou, Henan Province, China.
authors.forename: Kaka
authors.initials: K
authors.lastname: Katiella
authors.affiliation: Institute of Clinical Anatomy, Southern Medical University, Guangzhou, Guangdong Province, China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Huang
keywords: autologous nerve; chemically extracted acellular allogeneic nerve; ciliary neurotrophic factor; defect; nerve regeneration; neural regeneration; peripheral nerve injury; repair; transplantation