title: Synthesis of tumor-associated MUC1-glycopeptides and their multivalent presentation by functionalized gold colloids.
abstract: The mucin MUC1 is a glycoprotein involved in fundamental biological processes, which can be found over-expressed and with a distinctly altered glycan pattern on epithelial tumor cells; thus it is a promising target structure in the quest for effective carbohydrate-based cancer vaccines and immunotherapeutics. Natural glycopeptide antigens indicate only a low immunogenicity and a T-cell independent immune response; however, this major drawback can be overcome by coupling of glycopeptide antigens multivalently to immunostimulating carrier platforms. In particular, gold nanoparticles are well suited as templates for the multivalent presentation of glycopeptide antigens, due to their remarkably high surface-to-volume ratio in combination with their high biostability. In this work the synthesis of novel MUC1-glycopeptide antigens and their coupling to gold nanoparticles of different sizes are presented. In addition, the development of a new dot-blot immunoassay to test the potential antigen-antibody binding is introduced.
authors.affiliation: Institute of Inorganic and Analytical Chemistry, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 58, 35392 Giessen, Germany. Sabine.Schlecht@anorg.chemie.uni-giessen.de.
authors.forename: Isabella
authors.initials: I
authors.lastname: Tavernaro
authors.forename: Sebastian
authors.initials: S
authors.lastname: Hartmann
authors.forename: Laura
authors.initials: L
authors.lastname: Sommer
authors.forename: Heike
authors.initials: H
authors.lastname: Hausmann
authors.forename: Christian
authors.initials: C
authors.lastname: Rohner
authors.forename: Martin
authors.initials: M
authors.lastname: Ruehl
authors.forename: Anja
authors.initials: A
authors.lastname: Hoffmann-Roeder
authors.forename: Sabine
authors.initials: S
authors.lastname: Schlecht
mesh_terms: D060326:Chemistry Techniques, Synthetic; D006020:Glycopeptides / Q000138:chemical synthesis* / Q000737:chemistry* / Q000276:immunology; D017979:Gold Colloid / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry / Q000276:immunology; D007118:Immunoassay; D018396:Mucin-1 / Q000737:chemistry* / Q000276:immunology; D058525:Quartz Crystal Microbalance Techniques
chemical_list: D006020:Glycopeptides; D017979:Gold Colloid; D055767:Immobilized Proteins; D018396:Mucin-1