title: Blood transcriptomic biomarkers in adult primary care patients with major depressive disorder undergoing cognitive behavioral therapy.
abstract: An objective, laboratory-based diagnostic tool could increase the diagnostic accuracy of major depressive disorders (MDDs), identify factors that characterize patients and promote individualized therapy. The goal of this study was to assess a blood-based biomarker panel, which showed promise in adolescents with MDD, in adult primary care patients with MDD and age-, gender- and race-matched nondepressed (ND) controls. Patients with MDD received cognitive behavioral therapy (CBT) and clinical assessment using self-reported depression with the Patient Health Questionnaire-9 (PHQ-9). The measures, including blood RNA collection, were obtained before and after 18 weeks of CBT. Blood transcript levels of nine markers of ADCY3, DGKA, FAM46A, IGSF4A/CADM1, KIAA1539, MARCKS, PSME1, RAPH1 and TLR7, differed significantly between participants with MDD (N=32) and ND controls (N=32) at baseline (q< 0.05). Abundance of the DGKA, KIAA1539 and RAPH1 transcripts remained significantly different between subjects with MDD and ND controls even after post-CBT remission (defined as PHQ-9 <5). The ROC area under the curve for these transcripts demonstrated high discriminative ability between MDD and ND participants, regardless of their current clinical status. Before CBT, significant co-expression network of specific transcripts existed in MDD subjects who subsequently remitted in response to CBT, but not in those who remained depressed. Thus, blood levels of different transcript panels may identify the depressed from the nondepressed among primary care patients, during a depressive episode or in remission, or follow and predict response to CBT in depressed individuals.
authors.affiliation: 1] The Asher Center, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA [2] Department of Psychiatry and Behavioral Sciences, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
authors.forename: E E
authors.initials: EE
authors.lastname: Redei
authors.affiliation: Department of Psychiatry and Behavioral Sciences, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
authors.forename: B M
authors.initials: BM
authors.lastname: Andrus
authors.affiliation: 1] Center for Behavioral Intervention Technologies, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA [2] Department of Preventive Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Kwasny
authors.affiliation: 1] Department of Preventive Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA [2] School of Electrical Engineering, Korea University, Seoul, Korea.
authors.forename: J
authors.initials: J
authors.lastname: Seok
authors.affiliation: 1] Center for Behavioral Intervention Technologies, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA [2] Department of Preventive Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
authors.forename: X
authors.initials: X
authors.lastname: Cai
authors.affiliation: 1] Center for Behavioral Intervention Technologies, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA [2] Department of Preventive Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
authors.forename: J
authors.initials: J
authors.lastname: Ho
authors.affiliation: 1] Department of Psychiatry and Behavioral Sciences, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA [2] Center for Behavioral Intervention Technologies, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA [3] Department of Preventive Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
authors.forename: D C
authors.initials: DC
authors.lastname: Mohr
mesh_terms: D000262:Adenylyl Cyclases / Q000097:blood / Q000235:genetics; D000328:Adult; D002352:Carrier Proteins / Q000097:blood / Q000235:genetics; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules / Q000097:blood / Q000235:genetics; D015928:Cognitive Behavioral Therapy; D003865:Major Depressive Disorder / Q000097:blood / Q000235:genetics* / Q000628:therapy*; D005260:Female; D005819:Genetic Markers; D006801:Humans; D007136:Immunoglobulins / Q000097:blood / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins / Q000097:blood / Q000235:genetics; D008297:Male; D008565:Membrane Proteins / Q000097:blood / Q000235:genetics; D008875:Middle Aged; D009124:Muscle Proteins / Q000097:blood / Q000235:genetics; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D011062:Polynucleotide Adenylyltransferase; D011320:Primary Health Care; D046988:Proteasome Endopeptidase Complex / Q000097:blood / Q000235:genetics; D011506:Proteins / Q000235:genetics; D051199:Toll-Like Receptor 7 / Q000097:blood / Q000235:genetics
chemical_list: C403514:CADM1 protein, human; D002352:Carrier Proteins; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D005819:Genetic Markers; D007136:Immunoglobulins; D047908:Intracellular Signaling Peptides and Proteins; C000619710:MARCKS protein, human; D008565:Membrane Proteins; D009124:Muscle Proteins; C085047:PSME1 protein, human; D011506:Proteins; C493339:RAPH1 protein, human; C495348:TLR7 protein, human; D051199:Toll-Like Receptor 7; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D011062:Polynucleotide Adenylyltransferase; C466099:TENT5A protein, human; D046988:Proteasome Endopeptidase Complex; D000262:Adenylyl Cyclases; C421784:adenylate cyclase 3