title: Temporal insight into the natural generation of a new reassortant porcine influenza virus in a swine holding.
abstract: The influenza A virus (IAV) subtypes H1N1, H3N2 and H1N2 are the most prevalent subtypes in swine in Italy. Reassortant influenza A viruses subtypes in swine appeared in European pig population. In particular reassortant viruses carrying genome segment from the pandemic H1N1 (H1N1pdm) are reported in many European countries, including Italy. In a 1000 sows farrow-to feeder farm, in Northern Italy, we isolated 10 IAVs from recurrent episodes of respiratory disease in 45-70 days-old pigs from September 2012 to June 2013. The antigenic and genetic characterization of the swine IAV isolates showed the contemporary circulation of H1N1 avian-like and H1N1pdm strains in the first outbreak. The analysis of the viruses isolated subsequently showed the circulation of H1N1pdm IAV and then the establishment of a new previously undescribed H1N1 reassortant strain with a pandemic derived hemagglutinin gene and all the other seven segments of swine H1N1 avian-like lineage.
authors.affiliation: OIE Reference Laboratory for Swine Influenza, Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia Romagna, Via Dei Mercati 13A, 43126 Parma, Italy. Electronic address: chiara.chiapponi@izsler.it.
authors.forename: Chiara
authors.initials: C
authors.lastname: Chiapponi
authors.affiliation: OIE Reference Laboratory for Swine Influenza, Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia Romagna, Via Dei Mercati 13A, 43126 Parma, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Baioni
authors.affiliation: Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia Romagna, Via Pitagora 2, 42124 Reggio Emilia, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Luppi
authors.affiliation: Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia Romagna, Virologia Via A, Bianchi 9, 25125 Brescia, Italy.
authors.forename: Ana
authors.initials: A
authors.lastname: Moreno
authors.affiliation: DVM, Via Adige 60, 36100 Vicenza, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Castellan
authors.affiliation: OIE Reference Laboratory for Swine Influenza, Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia Romagna, Via Dei Mercati 13A, 43126 Parma, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Foni
mesh_terms: D000818:Animals; D001483:Base Sequence; D062665:Epidemiological Monitoring / Q000662:veterinary*; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics*; D007558:Italy / Q000453:epidemiology; D008969:Molecular Sequence Data; D009976:Orthomyxoviridae Infections / Q000453:epidemiology / Q000662:veterinary*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D016865:Reassortant Viruses / Q000235:genetics*; D012120:Respiration Disorders / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000821:virology*
keywords: H1N1; Reassortant; Swine influenza A virus