title: Relationship between testicular lesion and hormone levels in male rats infected with Trypanosoma evansi.
abstract: The aim of this study was to evaluate the relationship between testicular lesions and hormone levels in rats experimentally infected with Trypanosoma evansi. For that, the measurement of reproductive hormones, histopathology and biomarkers of cellular injury were carried out in twenty-four animals, which were divided into two groups with 12 animals each. Group A was the negative control, or uninfected, while group B was composed by animals infected with T. evansi. Both groups were divided again into two other subgroups (n=6), from which serum and testicular fragments were collected on days 5 (A1 and B1) and 15 (A2 and B2) post-infection (PI). The morphological analysis showed increased alterations of head and tail of sperm in infected rats when compared with those of the control group. A significant reduction (P<0.01) in the levels of LH, FSH, testosterone and estradiol, associated with an increase in cortisol, was observed in serum of group B when compared with negative control. Additionally, NOx, lipid peroxidation and protein oxidation were enhanced in testicles, indicating the occurrence of cellular lesion. On histopathology, it was possible to observe testicular degeneration, among other disorders in infected animals. Therefore, based on these results, it is possible to conclude that the experimental infection with T. evansi caused changes in the levels of the main hormones of male rats associated with cellular injury.
authors.affiliation: Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors.forename: Luciana
authors.initials: L
authors.lastname: Faccio
authors.affiliation: Departamento de Zootecnia, Universidade do Estado de Santa Catarina, Chapecó, SC, Brasil.
authors.forename: Aleksandro S da
authors.initials: AS
authors.lastname: Silva
authors.affiliation: Departamento de Clínica de Pequenos Animais, Universidade Federal de Santa Maria, Hospital Veterinário, Santa Maria, RS, Brasil.
authors.forename: Alexandre A
authors.initials: AA
authors.lastname: Tonin
authors.affiliation: Santa Maria, Santa Maria, RS, Brasil.
authors.forename: Lucas
authors.initials: L
authors.lastname: Oberherr
authors.affiliation: Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors.forename: Lucas T
authors.initials: LT
authors.lastname: Gressler
authors.affiliation: Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors.forename: Camila B
authors.initials: CB
authors.lastname: Oliveira
authors.affiliation: Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors.forename: Dionatan T
authors.initials: DT
authors.lastname: Oliveira
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors.forename: Manuela B
authors.initials: MB
authors.lastname: Sangoi
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors.forename: Rafael N
authors.initials: RN
authors.lastname: Moresco
authors.affiliation: Departamento de Morfologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors.forename: Yasmin N
authors.initials: YN
authors.lastname: Samara
authors.affiliation: Departamento de Morfologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Veiga
authors.affiliation: Universidade Luterana do Brasil, Campus Santa Maria, Santa Maria, RS, Brasil.
authors.forename: Marta M M F
authors.initials: MM
authors.lastname: Duarte
authors.affiliation: Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors.forename: Silvia G
authors.initials: SG
authors.lastname: Monteiro
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D004195:Disease Models, Animal; D004958:Estradiol / Q000097:blood; D005640:Follicle Stimulating Hormone / Q000097:blood; D006854:Hydrocortisone / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D018512:Parasitemia; D011374:Progesterone / Q000097:blood; D017208:Rats, Wistar; D013094:Spermatozoa / Q000469:parasitology*; D013737:Testis / Q000469:parasitology* / Q000503:physiopathology; D014352:Trypanosomiasis / Q000097:blood* / Q000503:physiopathology
chemical_list: D015415:Biomarkers; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D006854:Hydrocortisone