title: Effect of γ-irradiation on structure and nutraceutical potential of β-D-glucan from barley (Hordeum vulgare).
abstract: This paper reports the characterization and potential antioxidant activity of β-D-glucan isolated from barley treated with γ-rays. The β-D-glucan was irradiated with 0, 2, 4 and 8 kGy by gamma ray. The samples were characterized by Fourier transform-infrared spectroscopy, gel permeation chromatography (GPC) and quantitative estimation by Megazyme β-D-glucan assay kit. The average molecular weight of non-irradiated β-D-glucan was 177 kDa that decreased to 79 kDa at 8 kGy. Antioxidant activity was evaluated by five complementary assays including DPPH, lipid peroxidation, reducing power, metal chelating ability and oxidative DNA damage assays. Further, the antiproliferative potential of irradiated β-D-glucan was tested against three human cancer cell lines including Colo-205, T47D and MCF7 using MTT assay. Irradiated β-D-glucan exhibited dose dependent cancer cell growth inhibition. In conclusion, the present study demonstrates that irradiation leads to the formation of low molecular weight β-D-glucan with enhanced antioxidant and antiproliferative activities.
authors.affiliation: Department of Food Science and Technology, University of Kashmir, India.
authors.forename: Asima
authors.initials: A
authors.lastname: Shah
authors.affiliation: Department of Food Science and Technology, University of Kashmir, India.
authors.forename: Mudasir
authors.initials: M
authors.lastname: Ahmad
authors.affiliation: Department of Food Science and Technology, University of Kashmir, India.
authors.forename: Bilal Ahmad
authors.initials: BA
authors.lastname: Ashwar
authors.affiliation: Department of Food Science and Technology, University of Kashmir, India. Electronic address: adil.gani@gmail.com.
authors.forename: Adil
authors.initials: A
authors.lastname: Gani
authors.affiliation: Department of Food Science and Technology, University of Kashmir, India.
authors.forename: Farooq Ahmad
authors.initials: FA
authors.lastname: Masoodi
authors.affiliation: Department of Food Science and Technology, University of Kashmir, India.
authors.forename: Idrees Ahmed
authors.initials: IA
authors.lastname: Wani
authors.affiliation: Department of Food Science and Technology, University of Kashmir, India.
authors.forename: Sajad Mohd
authors.initials: SM
authors.lastname: Wani
authors.affiliation: Department of Food Technology Guru Nanak Dev University, Amritsar, Punjab, India.
authors.forename: Asir
authors.initials: A
authors.lastname: Gani
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D019587:Dietary Supplements / Q000032:analysis*; D016166:Free Radical Scavengers / Q000494:pharmacology; D005720:Gamma Rays; D001467:Hordeum / Q000737:chemistry*; D006801:Humans; D007502:Iron Chelating Agents / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008970:Molecular Weight; D010084:Oxidation-Reduction / Q000187:drug effects / Q000528:radiation effects; D010851:Picrates / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014783:Viscosity; D047071:beta-Glucans / Q000737:chemistry* / Q000494:pharmacology* / Q000528:radiation effects
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D007502:Iron Chelating Agents; D010851:Picrates; D047071:beta-Glucans; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Nutraceutical potential; β-d-Glucan; γ- Irradiation