title: The single-probe: a miniaturized multifunctional device for single cell mass spectrometry analysis.
abstract: We have developed a new mass spectrometry (MS) technology, the Single-probe MS, capable of real-time, in situ metabolomic analysis of individual living cells. The Single-probe is a miniaturized multifunctional sampling and ionization device that is directly coupled to the mass spectrometer. With a sampling tip smaller than individual eukaryotic cells (<10 μm), the Single-probe can be inserted into single cells to sample the intracellular compounds for real-time MS analysis. We have used the Single-probe to detect several cellular metabolites and the anticancer small molecules paclitaxel, doxorubicin, and OSW-1 in individual cervical cancer cells (HeLa). Single cell mass spectrometry (SCMS) is an emerging scientific technology that could reshape the analytical science of many research disciplines, and the Single-probe MS technology is a novel method for SCMS that, through its accessible fabrication protocols, can be broadly applied to different research areas.
authors.affiliation: Department of Chemistry and Biochemistry, University of Oklahoma , Norman, Oklahoma 73019, United States.
authors.forename: Ning
authors.initials: N
authors.lastname: Pan
authors.forename: Wei
authors.initials: W
authors.lastname: Rao
authors.forename: Naga Rama
authors.initials: NR
authors.lastname: Kothapalli
authors.forename: Renmeng
authors.initials: R
authors.lastname: Liu
authors.forename: Anthony W G
authors.initials: AW
authors.lastname: Burgett
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000244:Adenosine Diphosphate / Q000032:analysis / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000032:analysis / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000032:analysis / Q000378:metabolism; D000970:Antineoplastic Agents / Q000032:analysis*; D002783:Cholestenones / Q000032:analysis; D004317:Doxorubicin / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods; D055442:Metabolome; D017239:Paclitaxel / Q000032:analysis; D010713:Phosphatidylcholines / Q000032:analysis / Q000378:metabolism; D012503:Saponins / Q000032:analysis; D059010:Single-Cell Analysis / Q000295:instrumentation* / Q000379:methods
chemical_list: D000970:Antineoplastic Agents; D002783:Cholestenones; D010713:Phosphatidylcholines; D012503:Saponins; C106408:OSW 1; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D004317:Doxorubicin; D000255:Adenosine Triphosphate; D017239:Paclitaxel