title: Effects of oral butyrate application on insulin signaling in various tissues of chickens.
abstract: The influence of butyrate on insulin signaling in chickens was studied because butyrate is produced during microbial fermentation in the large intestine of birds, and butyrate is widely used as a feed additive in animal production. Ross 308 broiler chickens received a daily intraingluvial bolus of sodium butyrate (0.25 g/kg body weight) on days 20-24 of life (n = 10). Plasma butyrate concentration increased after receiving oral butyrate treatment (P < 0.001). Oral butyrate application was associated with decreased protein expression of insulin receptor β subunit (IRβ) in liver (P = 0.008) and both abdominal (P = 0.003) and subcutaneous adipose tissue (P < 0.001), but with elevated IRβ expression in muscle (P = 0.045), assessed by Western blotting. The quantity of hepatic phosphatidyl-inositol-3-kinase was reduced in the butyrate-treated group (P = 0.007); further, mammalian target of rapamycin was downregulated by butyrate in liver (P < 0.001) and subcutaneous adipose tissue (P = 0.038). Oral butyrate application provoked reduced systemic insulin sensitivity in chickens, indicated by elevated fasting blood glucose and subsequently, insulin level. However, responses of insulin signaling cascade to butyrate were tissue specific, suggesting that butyrate could act on glucose shifting among tissues by selectively increasing the glucose uptake of skeletal muscle via IRβ upregulation.
authors.affiliation: Department of Physiology and Biochemistry, Faculty of Veterinary Science, Szent István University, H-1078 Budapest, Hungary. Electronic address: Matis.Gabor@aotk.szie.hu.
authors.forename: G
authors.initials: G
authors.lastname: Mátis
authors.affiliation: Department of Physiology and Biochemistry, Faculty of Veterinary Science, Szent István University, H-1078 Budapest, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Kulcsár
authors.affiliation: Department of Physiology, University of Veterinary Medicine, D-30173 Hannover, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Turowski
authors.affiliation: Research Institute for Animal Breeding, Nutrition and Meat Science, National Agricultural Research Center, H-2053 Herceghalom, Hungary.
authors.forename: H
authors.initials: H
authors.lastname: Fébel
authors.affiliation: Department of Physiology and Biochemistry, Faculty of Veterinary Science, Szent István University, H-1078 Budapest, Hungary.
authors.forename: Zs
authors.initials: Z
authors.lastname: Neogrády
authors.affiliation: Department of Physiology, University of Veterinary Medicine, D-30173 Hannover, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Huber
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000284:Administration, Oral; D000818:Animals; D001786:Blood Glucose; D020148:Butyric Acid / Q000008:administration & dosage / Q000494:pharmacology*; D002645:Chickens / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism; D011972:Receptor, Insulin / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D000099221:Protein Kinase C zeta
chemical_list: D001786:Blood Glucose; D020148:Butyric Acid; D007328:Insulin; D011493:Protein Kinase C; D011972:Receptor, Insulin; D058570:TOR Serine-Threonine Kinases; D000099221:Protein Kinase C zeta
keywords: Butyrate; Chicken; Insulin homeostasis; Insulin receptor; Short chain fatty acids