title: Trimethylamine and Organic Matter Additions Reverse Substrate Limitation Effects on the δ13C Values of Methane Produced in Hypersaline Microbial Mats.
abstract: Methane production has been observed in a number of hypersaline environments, and it is generally thought that this methane is produced through the use of noncompetitive substrates, such as the methylamines, dimethylsulfide and methanol. Stable isotope measurements of the produced methane have also suggested that the methanogens are operating under conditions of substrate limitation. Here, substrate limitation in gypsum-hosted endoevaporite and soft-mat hypersaline environments was investigated by the addition of trimethylamine, a noncompetitive substrate for methanogenesis, and dried microbial mat, a source of natural organic matter. The δ(13)C values of the methane produced after amendments were compared to those in unamended control vials. At all hypersaline sites investigated, the δ(13)C values of the methane produced in the amended vials were statistically lower (by 10 to 71‰) than the unamended controls, supporting the hypothesis of substrate limitation at these sites. When substrates were added to the incubation vials, the methanogens within the vials fractionated carbon isotopes to a greater degree, resulting in the production of more (13)C-depleted methane. Trimethylamine-amended samples produced lower methane δ(13)C values than the mat-amended samples. This difference in the δ(13)C values between the two types of amendments could be due to differences in isotope fractionation associated with the dominant methane production pathway (or substrate used) within the vials, with trimethylamine being the main substrate used in the trimethylamine-amended vials. It is hypothesized that increased natural organic matter in the mat-amended vials would increase fermentation rates, leading to higher H2 concentrations and increased CO2/H2 methanogenesis.
authors.affiliation: Department of Geological Sciences, University of Missouri, Columbia, Missouri, USA kelleyc@missouri.edu.
authors.forename: Cheryl A
authors.initials: CA
authors.lastname: Kelley
authors.affiliation: Department of Geological Sciences, University of Missouri, Columbia, Missouri, USA.
authors.forename: Brooke E
authors.initials: BE
authors.lastname: Nicholson
authors.affiliation: Department of Geological Sciences, University of Missouri, Columbia, Missouri, USA.
authors.forename: Claire S
authors.initials: CS
authors.lastname: Beaudoin
authors.affiliation: Exobiology Branch, NASA Ames Research Center, Moffett Field, California, USA.
authors.forename: Angela M
authors.initials: AM
authors.lastname: Detweiler
authors.affiliation: Exobiology Branch, NASA Ames Research Center, Moffett Field, California, USA.
authors.forename: Brad M
authors.initials: BM
authors.lastname: Bebout
mesh_terms: D000498:Allyl Compounds; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D019015:Geologic Sediments / Q000382:microbiology*; D006859:Hydrogen / Q000378:metabolism; D007553:Isotope Labeling; D008697:Methane / Q000378:metabolism*; D008744:Methylamines / Q000378:metabolism*; D013440:Sulfides
chemical_list: D000498:Allyl Compounds; D002247:Carbon Isotopes; D008744:Methylamines; D013440:Sulfides; D002245:Carbon Dioxide; D006859:Hydrogen; C028526:methylallyl trisulfide; C023336:trimethylamine; D008697:Methane