title: Hydrogen sulfide alleviates the aluminum-induced changes in Brassica napus as revealed by physiochemical and ultrastructural study of plant.
abstract: In the present study, ameliorating role of hydrogen sulfide (H2S) in oilseed rape (Brassica napus L.) was studied with or without application of H2S donor sodium hydrosulfide (NaHS) (0.3 mM) in hydroponic conditions under three levels (0, 0.1 and 0.3 mM) of aluminum (Al). Results showed that addition of H2S significantly improved the plant growth, photosynthetic gas exchange, and nutrients concentration in the leaves and roots of B. napus plants under Al stress. Exogenously applied H2S significantly lowered the Al concentration in different plant parts, and reduced the production of malondialdehyde and reactive oxygen species by improving antioxidant enzyme activities in the leaves and roots under Al stress. Moreover, the present study indicated that exogenously applied H2S improved the cell structure and displayed clean mesophyll and root tip cells. The chloroplast with well-developed thylakoid membranes could be observed in the micrographs. Under the combined application of H2S and Al, a number of modifications could be observed in root tip cell, such as mitochondria, endoplasmic reticulum, and golgi bodies. Thus, it can be concluded that exogenous application of H2S under Al stress improved the plant growth, photosynthetic parameters, elements concentration, and biochemical and ultrastructural changes in leaves and roots of B. napus.
authors.affiliation: Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Basharat
authors.initials: B
authors.lastname: Ali
authors.forename: Ping
authors.initials: P
authors.lastname: Qian
authors.forename: Rui
authors.initials: R
authors.lastname: Sun
authors.forename: Muhammad A
authors.initials: MA
authors.lastname: Farooq
authors.forename: Rafaqat A
authors.initials: RA
authors.lastname: Gill
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Muhammad
authors.initials: M
authors.lastname: Azam
authors.forename: Weijun
authors.initials: W
authors.lastname: Zhou
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D000818:Animals; D029688:Brassica napus / Q000254:growth & development* / Q000378:metabolism* / Q000648:ultrastructure; D002736:Chloroplasts / Q000648:ultrastructure; D006862:Hydrogen Sulfide / Q000378:metabolism* / Q000494:pharmacology; D018527:Hydroponics; D015227:Lipid Peroxidation / Q000502:physiology; D008315:Malondialdehyde / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D010788:Photosynthesis / Q000187:drug effects*; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013440:Sulfides / Q000494:pharmacology
chemical_list: D017382:Reactive Oxygen Species; D013440:Sulfides; D008315:Malondialdehyde; D000535:Aluminum; C025451:sodium bisulfide; D006862:Hydrogen Sulfide