title: Cellulosomal carbohydrate-binding module from Clostridium josui binds to crystalline and non-crystalline cellulose, and soluble polysaccharides.
abstract: To understand the lignocellulose degradation activity of the Clostridium josui cellulosome, a carbohydrate-binding module of the scaffoldin CjCBM3 was characterized. CjCBM3 shows binding to crystalline cellulose, non-crystalline cellulose and soluble polysaccharides. The binding isotherm of CjCBM3 to acid-swollen cellulose is best fitted by the Langmuir two-site model, suggesting that there are two CjCBM3 binding sites on acid-swollen cellulose with different affinities. The second site shows lower affinity and larger binding capacity, suggesting that the cellulosome is directly targeted to the cellulose surface with high affinity, where larger amounts of the cellulosome bind to cellulose with low affinity.
authors.affiliation: Graduate School of Regional Innovation Studies, Mie University, 1577 Kurimamachiya, Tsu 514-8507, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Ichikawa
authors.affiliation: Graduate School of Regional Innovation Studies, Mie University, 1577 Kurimamachiya, Tsu 514-8507, Japan. Electronic address: karita@bio.mie-u.ac.jp.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Karita
authors.affiliation: Graduate School of Bioresources, Mie University, 1577 Kurimamachiya, Tsu 514-8507, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kondo
authors.affiliation: Graduate School of Bioresources, Mie University, 1577 Kurimamachiya, Tsu 514-8507, Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Goto
mesh_terms: D000327:Adsorption; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D044603:Cellulosomes / Q000378:metabolism*; D003013:Clostridium / Q000166:cytology* / Q000378:metabolism; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D012995:Solubility
chemical_list: D001426:Bacterial Proteins; D011134:Polysaccharides; C036909:lignocellulose; D008031:Lignin
keywords: Carbohydrate-binding module; Cellulosome; Lignocellulose degradation; Scaffoldin