title: RING finger proteins are involved in the progression of barrett esophagus to esophageal adenocarcinoma: a preliminary study.
abstract: OBJECTIVE
  To investigate the differential expression of RING finger (RNF) proteins in Barrett esophagus (BE) and esophageal adenocarcinoma (EAC).
  
  
  METHODS
  The differential expression of RNFs in normal esophagus (NE), BE, and EAC was screened using microarray assay. Real-time quantitative polymerase chain reaction (PCR), tissue micro-array assay, and Western blot analysis were independently performed to detect the mRNA and protein expression of screened RNFs.
  
  
  RESULTS
  The expression of nine RNFs in the BE or EAC was 2-fold higher than those in NE. Among these proteins, the RNF32 and RNF121 expression in BE was 20.3-fold and 16.4-fold higher, respectively, than that in NE, and the expression of RNF24, RNF130, RNF141, RNF139, RNF11, RNF14, and RNF159 was upregulated more than 2-fold compared with NE. The expression of nine RNFs was not only upregulated in the EAC but was also positively related to the RNF expression in BE. The PCR results also indicated increased expression of these RNFs in BE and EAC compared to NE. Furthermore, the mRNA expression of all RNFs, except for RNF141 in EAC, was dramatically higher than those in the BE. Similar results were also obtained from the Western blot analysis.
  
  
  CONCLUSIONS
  A total of nine RNFs play critical roles in the progression of BE to EAC.
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China ; Gastroenterology Research Institute, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Xing Wei
authors.initials: XW
authors.lastname: Wang
authors.affiliation: Department of Trauma and Microsurgery, 324 Hospital of People's Liberation Army, Chongqing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Department of Gastroenterology, 281 Hospital of People's Liberation Army, Qinhuangdao, China.
authors.forename: Wei Qiang
authors.initials: WQ
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Xiao Yan
authors.initials: XY
authors.lastname: Zhao
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Guo
authors.affiliation: Gastroenterology Research Institute, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Dian Chun
authors.initials: DC
authors.lastname: Fang
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000235:genetics; D000328:Adult; D000368:Aged; D001471:Barrett Esophagus / Q000201:enzymology* / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D004268:DNA-Binding Proteins; D018450:Disease Progression; D004938:Esophageal Neoplasms / Q000201:enzymology* / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D008297:Male; D008875:Middle Aged; D011506:Proteins / Q000235:genetics; D054829:RING Finger Domains; D011956:Receptors, Cell Surface / Q000235:genetics; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; C404508:RNF11 protein, human; C114025:RNF139 protein, human; C118499:RNF14 protein, human; C452818:RNF32 protein, human; D011956:Receptors, Cell Surface; C511931:RNF130 protein, human; C530470:RNF24 protein, human; D044767:Ubiquitin-Protein Ligases
keywords: Barrett esophagus; Esophageal adenocarcinoma; RING finger proteins; Tissue array analysis