title: Isolation, screening and partial purification of antimicrobial antibiotics from soil Streptomyces sp. SCA 7.
abstract: Thirty-seven actinomycetes strains were isolated from soil samples collected from an agriculture field in Vengodu, Thiruvannamalai District, Tamil Nadu, India (latitude: 12° 54' 0033″, North; longitude: 79° 78' 5216″, East; elevation: 228.6/70.0 ft/m). The isolates were assessed for antagonistic activity against five Gram-positive bacteria, seven Gram-negative bacteria, and two pathogenic fungi. During the initial screening, 43% of the strains showed weak activity, 16% showed moderate activity, 5% showed good activity, and 35% showed no antagonistic activity. Among the strains tested, SCA 7 showed strong antimicrobial activity. Maximum biological activity was obtained on modified nutrient glucose agar (MNGA) medium. The mycelia of SCA 7 were extracted with methanol and tested against microbial pathogens using the disc diffusion method. The crude extract was purified partially using column chromatography and assessed for antimicrobial activity. Fraction 10 showed good activity against Staphylococcus epidermidis (31.25 μg/mL) and Malassezia pachydermatis (500 μg/mL) and the active principle (fraction 10) was identified as 2,4-bis (1,1-dimethylethyl) phenol. Based on morphological, physiological, biochemical, cultural, and molecular characteristics (16S rDNA sequencing), this strain was identified as Streptomyces sp. SCA 7. It could be used in the development of new substances for pharmaceutical or agricultural purposes.
authors.affiliation: Division of Microbiology, Entomology Research Institute, Loyola College, Chennai, India.
authors.forename: P
authors.initials: P
authors.lastname: Saravana Kumar
authors.affiliation: Department of Botany and Microbiology, College of Science, King Saud University, P.O.Box.2455, Riyadh 11451, Saudi Arabia.
authors.forename: V
authors.initials: V
authors.lastname: Duraipandiyan
authors.affiliation: Division of Microbiology, Entomology Research Institute, Loyola College, Chennai, India. Electronic address: entolc@hotmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Ignacimuthu
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005591:Chemical Fractionation; D003470:Culture Media; D004275:DNA, Ribosomal / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013302:Streptomyces / Q000737:chemistry* / Q000166:cytology / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D004275:DNA, Ribosomal
keywords: Actinomycetes; Antimicrobial activity; Methanolic extract; Minimum inhibitory concentration; Streptomyces sp. SCA 7