title: Sensitive quantification of messenger RNA with a real-time ligase chain reaction by using a ribonucleotide-modified DNA probe.
abstract: A highly sensitive and specific assay is developed for quantification of messenger RNA with a real-time fluorescence ligase chain reaction by using a ribonucleotide-modified DNA probe.
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province, P. R. China. lzpbd@snnu.edu.cn.
authors.forename: Yanlei
authors.initials: Y
authors.lastname: Hu
authors.forename: Hongxia
authors.initials: H
authors.lastname: Jia
authors.forename: Yucong
authors.initials: Y
authors.lastname: Wang
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Cheng
authors.forename: Zhengping
authors.initials: Z
authors.lastname: Li
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D015342:DNA Probes / Q000737:chemistry / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D006367:HeLa Cells; D006801:Humans; D022521:Ligase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis* / Q000378:metabolism
chemical_list: D000199:Actins; D015342:DNA Probes; D012333:RNA, Messenger