title: Omega-3 fatty acids reduce obesity-induced tumor progression independent of GPR120 in a mouse model of postmenopausal breast cancer.
abstract: Obesity and inflammation are both risk factors for a variety of cancers, including breast cancer in postmenopausal women. Intake of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) decreases the risk of breast cancer, and also reduces obesity-associated inflammation and insulin resistance, but whether the two effects are related is currently unknown. We tested this hypothesis in a postmenopausal breast cancer model using ovariectomized, immune-competent female mice orthotopically injected with Py230 mammary tumor cells. Obesity, whether triggered genetically or by high-fat diet (HFD) feeding, increased inflammation in the mammary fat pad and promoted mammary tumorigenesis. The presence of tumor cells in the mammary fat pad further enhanced the local inflammatory milieu. Tumor necrosis factor-alpha (TNF-α) was the most highly upregulated cytokine in the obese mammary fat pad, and we observed that TNF-α dose-dependently stimulated Py230 cell growth in vitro. An ω-3 PUFA-enriched HFD (referred to as fish oil diet, FOD) reduced inflammation in the obese mammary fat pad in the absence of tumor cells and inhibited Py230 tumor growth in vivo. Although some anti-inflammatory effects of ω-3 PUFAs were previously shown to be mediated by the G-protein-coupled receptor 120 (GPR120), the FOD reduced Py230 tumor burden in GPR120-deficient mice to a similar degree as observed in wild-type mice, indicating that the effect of FOD to reduce tumor growth does not require GPR120 in the host mouse. Instead, in vitro studies demonstrated that ω-3 PUFAs act directly on tumor cells to activate c-Jun N-terminal kinase, inhibit proliferation and induce apoptosis. Our results show that obesity promotes mammary tumor progression in this model of postmenopausal breast cancer and that ω-3 PUFAs, independent of GPR120, inhibit mammary tumor progression in obese mice.
authors.affiliation: Division of Endocrinology and Metabolism, Department of Medicine, University of California, La Jolla, CA, USA.
authors.forename: H
authors.initials: H
authors.lastname: Chung
authors.affiliation: Division of Endocrinology and Metabolism, Department of Medicine, University of California, La Jolla, CA, USA.
authors.forename: Y S
authors.initials: YS
authors.lastname: Lee
authors.affiliation: 1] Division of Endocrinology and Metabolism, Department of Medicine, University of California, La Jolla, CA, USA [2] Biomedical Network Center for the study of Hepatic and Digestive Diseases (CIBERehd), Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Mayoral
authors.affiliation: Division of Endocrinology and Metabolism, Department of Medicine, University of California, La Jolla, CA, USA.
authors.forename: D Y
authors.initials: DY
authors.lastname: Oh
authors.affiliation: Department of Pathology, University of California at San Diego, La Jolla, CA, USA.
authors.forename: J T
authors.initials: JT
authors.lastname: Siu
authors.affiliation: 1] Division of Endocrinology and Metabolism, Department of Medicine, University of California, La Jolla, CA, USA [2] Moores Cancer Center, University of California, La Jolla, CA, USA [3] San Diego VA Healthcare System, San Diego, CA, USA.
authors.forename: N J
authors.initials: NJ
authors.lastname: Webster
authors.affiliation: 1] Division of Endocrinology and Metabolism, Department of Medicine, University of California, La Jolla, CA, USA [2] Moores Cancer Center, University of California, La Jolla, CA, USA.
authors.forename: D D
authors.initials: DD
authors.lastname: Sears
authors.affiliation: 1] Division of Endocrinology and Metabolism, Department of Medicine, University of California, La Jolla, CA, USA [2] Moores Cancer Center, University of California, La Jolla, CA, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Olefsky
authors.affiliation: 1] Department of Pathology, University of California at San Diego, La Jolla, CA, USA [2] Moores Cancer Center, University of California, La Jolla, CA, USA.
authors.forename: L G
authors.initials: LG
authors.lastname: Ellies
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D059305:Diet, High-Fat; D018450:Disease Progression; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000150:complications* / Q000235:genetics / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000150:complications* / Q000378:metabolism; D010052:Ovariectomy; D017698:Postmenopause / Q000502:physiology; D043562:Receptors, G-Protein-Coupled / Q000502:physiology*
chemical_list: C498007:FFAR4 protein, mouse; D015525:Fatty Acids, Omega-3; D043562:Receptors, G-Protein-Coupled