title: Activation of TGFβ1 signaling enhances early dopaminergic differentiation in unrestricted somatic stem cells.
abstract: So far there is increasing evidence for the involvement of transforming growth factor beta TGFβ (transforming growth factor) in differentiation and maintenance of midbrain dopaminergic neurons. Considering that USSCs (unrestricted somatic stem cells) have the potentials to differentiate into neuron-like cells and even dopaminergic neurons and that no evidence available on the role of TGFβ signaling in dopaminergic differentiation of these cells, we investigated the presence of TGFβ signaling components in USSCs and their involvement on USSCs differentiation into early dopaminergic neurons. Our results showed that components of TGFβ signaling were present and functional in undifferentiated USSCs, after which the neurally induced USSCs treated with TGFβ1 for 3 days resulted in increased expression of β-tubulin III (a general neuronal marker) and Nurr-1 (an early dopaminergic marker) at both mRNA and protein levels. Consistently, TGFβ inhibition in culture medium by using SB431542 in the presence or absence of TGFβ1, significantly decreased the expression of both neural markers. We therefore suggest that activation of TGFβ signaling-pathway in neurally induced USSCs enhances neural differentiation with an early dopaminergic phenotype which points at the positive role of the TGFβ signaling pathway toward dopaminergic fate.
authors.affiliation: Developmental Biology Lab, School of Biology, College of Science, University of Tehran, Tehran 14155-6455, Iran.
authors.forename: Akram Mokhtarzadeh
authors.initials: AM
authors.lastname: Khanghahi
authors.affiliation: Developmental Biology Lab, School of Biology, College of Science, University of Tehran, Tehran 14155-6455, Iran. Electronic address: zeynalib@ut.ac.ir.
authors.forename: Bahman
authors.initials: B
authors.lastname: Zeynali
authors.affiliation: Developmental Biology Lab, School of Biology, College of Science, University of Tehran, Tehran 14155-6455, Iran.
authors.forename: Azimeh
authors.initials: A
authors.lastname: Akhlaghpoor
authors.affiliation: Department of Molecular Medicine, Faculty of Medical Biotechnology, Research Institute of Genetic Engineering and Biotechnology, Tehran 14965-161, Iran.
authors.forename: Azita Parvaneh
authors.initials: AP
authors.lastname: Tafreshi
authors.affiliation: Institute for Anatomy and Cell Biology, Department of Molecular Embryology, Albert-Ludwigs-Universität, Freiburg, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Krieglstein
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D059290:Dopaminergic Neurons / Q000166:cytology* / Q000378:metabolism; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2 / Q000378:metabolism; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism
chemical_list: C539882:NR4A2 protein, human; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2; D053773:Transforming Growth Factor beta1; D014404:Tubulin
keywords: Dopaminergic differentiation; TGFβ signaling; USSC