title: In vivo formaldehyde cross-linking: it is time for black box analysis.
abstract: Formaldehyde cross-linking is an important component of many technologies, including chromatin immunoprecipitation and chromosome conformation capture. The procedure remains empirical and poorly characterized, however, despite a long history of its use in research. Little is known about the specificity of in vivo cross-linking, its efficiency and chemical adducts induced by the procedure. It is time to search this black box.
authors.forename: Alexey
authors.initials: A
authors.lastname: Gavrilov
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Razin
authors.forename: Giacomo
authors.initials: G
authors.lastname: Cavalli
mesh_terms: D047369:Chromatin Immunoprecipitation; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D022041:Euchromatin / Q000378:metabolism; D005557:Formaldehyde / Q000494:pharmacology*; D006570:Heterochromatin / Q000378:metabolism; D006801:Humans; D012441:Saccharomyces cerevisiae / Q000378:metabolism
chemical_list: D003432:Cross-Linking Reagents; D022041:Euchromatin; D006570:Heterochromatin; D005557:Formaldehyde
keywords: 3C; 3D genome organization; ChIP; chromatin; formaldehyde cross-linking