title: A new family of iron-dependent halogenases acts on freestanding substrates.
abstract: Regio- and stereospecific incorporation of a halogen atom to an unactivated sp(3) carbon in a freestanding molecule is a challenging transformation that is currently missing in the inventory of enzyme-mediated reactions. Here we report what is to our knowledge the first example of a nonheme iron enzyme (WelO5) in the welwitindolinone biosynthetic pathway that can monochlorinate an aliphatic carbon in 12-epi-fischerindole U and 12-epi-hapalindole C, substrates that are free from peptidyl or acyl carrier protein.
authors.affiliation: Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Hillwig
authors.affiliation: Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Liu
mesh_terms: D006219:Halogens / Q000737:chemistry / Q000378:metabolism*; D026121:Indole Alkaloids / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D008968:Molecular Conformation; D010088:Oxidoreductases / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D006219:Halogens; D026121:Indole Alkaloids; C507638:welwitindolinone A; D007501:Iron; D010088:Oxidoreductases