title: A novel monoclonal antibody to Neisseria meningitidis serogroup X capsular polysaccharide and its potential use in quantitation of meningococcal vaccines.
abstract: A novel murine hybridoma monoclonal antibody (MAb) was produced against the capsular polysaccharide (CP) of Neisseria meningitidis serogroup X (MenX) in order to develop a sandwich enzyme linked immunosorbent assay (ELISA) for the quantitation of the meningococcal polysaccharide. The MAb only reacted with the CP from MenX and did not react with CPs from N. meningitidis serogroups A, C, Y and W (MenA, MenC, MenY, MenW). The affinity constant (Ka) of the MAb measured by non-competitive ELISA was 7.25 × 10(7) M(-1). The application of this MAb in a sandwich ELISA was demonstrated by its ability to properly quantitate three lots of an experimental meningococcal CP-based vaccine. The MAb obtained in this work could be a valuable reagent for the detection and quantitation of future meningococcal vaccines containing MenX CP.
authors.affiliation: Research and Development Vicepresidency, Finlay Institute, La Habana, A.P. 16017, Cod. 11600, Cuba; Department of Pharmacology, Faculty of Biological Sciences, University of Concepción, Concepción, P.O. Box 160C, Chile. Electronic address: fatima8526@gmail.com.
authors.forename: Fátima
authors.initials: F
authors.lastname: Reyes
authors.affiliation: Research and Development Vicepresidency, Finlay Institute, La Habana, A.P. 16017, Cod. 11600, Cuba.
authors.forename: Oscar
authors.initials: O
authors.lastname: Otero
authors.affiliation: Research and Development Vicepresidency, Finlay Institute, La Habana, A.P. 16017, Cod. 11600, Cuba; Department of Pharmacology, Faculty of Biological Sciences, University of Concepción, Concepción, P.O. Box 160C, Chile.
authors.forename: Frank
authors.initials: F
authors.lastname: Camacho
authors.affiliation: Research and Development Vicepresidency, Finlay Institute, La Habana, A.P. 16017, Cod. 11600, Cuba.
authors.forename: Nevis
authors.initials: N
authors.lastname: Amin
authors.affiliation: Research and Development Vicepresidency, Finlay Institute, La Habana, A.P. 16017, Cod. 11600, Cuba.
authors.forename: Fidel
authors.initials: F
authors.lastname: Ramírez
authors.affiliation: Research and Development Vicepresidency, Finlay Institute, La Habana, A.P. 16017, Cod. 11600, Cuba.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Valdés
authors.affiliation: Research and Development Vicepresidency, Finlay Institute, La Habana, A.P. 16017, Cod. 11600, Cuba.
authors.forename: Reynaldo
authors.initials: R
authors.lastname: Acevedo
authors.affiliation: Research and Development Vicepresidency, Finlay Institute, La Habana, A.P. 16017, Cod. 11600, Cuba.
authors.forename: Luis
authors.initials: L
authors.lastname: García
authors.affiliation: Research and Development Vicepresidency, Finlay Institute, La Habana, A.P. 16017, Cod. 11600, Cuba.
authors.forename: Daniel
authors.initials: D
authors.lastname: Cardoso
authors.affiliation: Research and Development Vicepresidency, Finlay Institute, La Habana, A.P. 16017, Cod. 11600, Cuba.
authors.forename: Maribel
authors.initials: M
authors.lastname: Cuello
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002138:Calibration; D004797:Enzyme-Linked Immunosorbent Assay; D057230:Limit of Detection; D008589:Meningococcal Infections / Q000382:microbiology / Q000517:prevention & control*; D022401:Meningococcal Vaccines / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D009345:Neisseria meningitidis / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry*; D065288:Serogroup; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; D022401:Meningococcal Vaccines; D011134:Polysaccharides
keywords: ELISA; Monoclonal antibody; Neisseria meningitidis serogroup X; Quantitation