title: DNA vaccine encoding Haemonchus contortus actin induces partial protection in goats.
abstract: Actin is a globular multi-functional protein that forms microfilaments, and participates in many important cellular processes. Previous study found that Haemonchus contortus actin could be recognized by the serum of goats infected with the homology parasite. This indicated that H. contortus actin could be a potential candidate for vaccine. In this study, DNA vaccine encoding H. contortus actin was tested for protection against experimental H. contortus infections in goats. Fifteen goats were allocated into three trial groups. The animals of Actin group were vaccinated with the DNA vaccine on day 0 and 14, and challenged with 5000 infective H. contortus third stage larval (L3) on day 28. An unvaccinated positive control group was challenged with L3 at the same time. An unvaccinated negative control group was not challenged with L3. The results showed that DNA vaccine were transcribed at local injection sites and expressed in vivo post immunizations respectively. For goats in Actin vaccinated group, higher levels of serum IgG, serum IgA and mucosal IgA were produced, the percentages of CD4(+) T lymphocytes, CD8(+) T lymphocytes and B lymphocytes and the concentrations of TGF-β were increased significantly (P<0.05). Following L3 challenge, the mean eggs per gram feces (EPG) and worm burdens of Actin group were reduced by 34.4% and 33.1%, respectively. This study suggest that recombinant H. contortus Actin DNA vaccine induced partial immune response and has protective potential against goat haemonchosis.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Ruofeng
authors.initials: R
authors.lastname: Yan
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.forename: Lixin
authors.initials: L
authors.lastname: Xu
authors.forename: Xiaokai
authors.initials: X
authors.lastname: Song
authors.forename: Xiangrui
authors.initials: X
authors.lastname: Li
mesh_terms: D000199:Actins / Q000276:immunology; D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D015511:Goat Diseases / Q000469:parasitology* / Q000517:prevention & control; D006041:Goats; D006188:Haemonchiasis / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D006189:Haemonchus / Q000276:immunology*; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D016131:Lymphocyte Subsets; D008297:Male; D014612:Vaccines / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology
chemical_list: D000199:Actins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D014612:Vaccines; D019444:Vaccines, DNA