title: Differentiation of mesenchymal stem cells into neural stem cells using cerebrospinal fluid.
abstract: Optimization of a methodology for mesenchymal stem cells (MSCs) differentiation into neural stem cells (NSCs) using cerebrospinal fluid (CSF). MSCs were extracted from umbilical cord blood from healthy, full-term, newborn infants and from the bone marrow of patients. CSF was taken from healthy adult volunteers and patients. Four groups investigated were: A (n = 8) cord blood MSC induced with healthy volunteer CSF (control group); B (n = 7): patient MSCs induced with health volunteer CSF; Group C (n = 12): patient MSCs induced with their own CSF; group D (n = 6): cord blood MSCs induced with patient CSF. Following induction, cell differentiation state was examined using microscopy, flow cytometry, and immunohistochemistry. There were significantly more clinically applicable MSCs in Groups B and C than groups A and D (P < 0.05) and Group B had significantly more clinically applicable MSCs than group C (P < 0.05). The presence of NSCs was as with the MSCs. Group B had significantly more clinically applicable NSCs than all of the other groups. In addition, group B cells grew significantly faster than the other groups (P < 0.05). Upon CSF induction, MSCs differentiated into NSCs suitable for clinical treatment. The source of the MSCs and/or CSF influenced the number of NSCs produced and the NSC growth rate. Thus, the source of MSCs and CSF should be considered before initiating a stem cell clinical treatment.
authors.affiliation: Department of Neurology, Affiliated Hospital of Xuzhou Medical College, 99 Huaihai Road West, Xuzhou, 221002, Jiangsu, China, gw1003@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Ge
authors.forename: Chao
authors.initials: C
authors.lastname: Ren
authors.forename: Xin
authors.initials: X
authors.lastname: Duan
authors.forename: Deqin
authors.initials: D
authors.lastname: Geng
authors.forename: Caiyi
authors.initials: C
authors.lastname: Zhang
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Liu
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Meirong
authors.initials: M
authors.lastname: Wan
authors.forename: Runlu
authors.initials: R
authors.lastname: Geng
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002555:Cerebrospinal Fluid / Q000378:metabolism*; D003584:Cytological Techniques / Q000379:methods*; D006801:Humans; D007223:Infant; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D015415:Biomarkers