title: Evaluation of antitumoral and antimicrobial activity of Morinda lcitrifolia L. grown in Southeast Brazil.
abstract: OBJECTIVE
  To evaluate the antitumor and antimicrobial activity of ethanolic extract of Morinda citrifolia L. fruit cultivated in southeastern Brazil.
  
  
  METHODS
  Preparation ethanolic extract of the fruit of Morinda citrifolia L. Culture of melanoma cells B16-F10 for treatment with ethanolic extract of Morinda citrifolia L. fruit to determine cell viability by MTT and determination temporal effect of ethanolic extract fruit on the cell growth B16-F10 for 8 days. Evaluation of antimicrobial activity of ethanolic extract fruit against Staphylococcus aureus and Escherichia coli by determination of Minimum Inhibitory Concentration (MIC).
  
  
  RESULTS
  The ethanolic extract of Morinda citrifolia L. fruit (10mg/mL) decreased cellular activity and inhibited 45% the rate of cell proliferation of B16-F10 melanoma treated during period studied. The ethanolic extract of Morinda citrifolia L. fruit demonstrated antimicrobial activity inhibiting the growth of both microorganisms studied. Staphylococcus aureus was less resistant to ethanolic extract of Morinda citrifolia L. fruit than Escherichia coli, 1 mg/mL and 10 mg/mL, respectively.
  
  
  CONCLUSIONS
  What these results indicate that the ethanolic extract of the fruit of Morinda citrifolia L. showed antitumor activity with inhibition of viability and growth of B16-F10 cells and also showed antibacterial activity as induced inhibition of growth of Staphylococcus aureus and Escherichia coli.
authors.affiliation: Department of Biological Sciences, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil.
authors.forename: Thamyris
authors.initials: T
authors.lastname: Candida
authors.affiliation: Department of Biological Sciences, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil.
authors.forename: Jerônimo Pereira de
authors.initials: JP
authors.lastname: França
authors.affiliation: Department of Biological Sciences, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil.
authors.forename: Alba Lucilvânia Fonseca
authors.initials: AL
authors.lastname: Chaves
authors.affiliation: Department of Biological Sciences, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil.
authors.forename: Fernanda Andrade Rodrigues
authors.initials: FA
authors.lastname: Lopes
authors.affiliation: Department of Biological Sciences, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil.
authors.forename: Silvana
authors.initials: S
authors.lastname: Gaiba
authors.affiliation: Department of Agricultural and Environmental Sciences, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil.
authors.forename: Celio Kersul do
authors.initials: CK
authors.lastname: Sacramento
authors.affiliation: Department of Agricultural and Environmental Sciences, Universidade Estadual de Santa Cruz, Sao Paulo, SP, Brazil.
authors.forename: Lydia Masako
authors.initials: LM
authors.lastname: Ferreira
authors.affiliation: Department of Biological Sciences, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil.
authors.forename: Lucimar Pereira de
authors.initials: LP
authors.lastname: França
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D001938:Brazil; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000187:drug effects; D000431:Ethanol; D005638:Fruit / Q000737:chemistry; D008545:Melanoma / Q000188:drug therapy; D051379:Mice; D008826:Microbial Sensitivity Tests; D032066:Morinda / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D015203:Reproducibility of Results; D013211:Staphylococcus aureus / Q000187:drug effects; D013997:Time Factors
chemical_list: D000890:Anti-Infective Agents; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D000431:Ethanol