title: Extraction, preliminary characterization and evaluation of in vitro antitumor and antioxidant activities of polysaccharides from Mentha piperita.
abstract: This study describes the extraction, preliminary characterization and evaluation of the in vitro antitumor and antioxidant activities of polysaccharides extracted from Mentha piperita (MPP). The optimal parameters for the extraction of MPP were obtained by Box-Behnken experimental design and response surface methodology (RSM) at the ratio of water to raw material of 20, extraction time of 1.5 h and extraction temperature at 80 °C. Chemical composition analysis showed that MPP was mainly composed of glucuronic acid, galacturonic acid, glucose, galactose and arabinose, and the molecular weight of its two major fractions were estimated to be about 2.843 and 1.139 kDa, respectively. In vitro bioactivity experiments showed that MPP not only inhibited the growth of A549 cells but possessed potent inhibitory action against DNA topoisomerase I (topo I), and an appreciative antioxidant action as well. These results indicate that MPP may be useful for developing safe natural health products.
authors.affiliation: Biology Institute of Shandong Academy of Sciences/Key Laboratory for Applied Microbiology of Shandong Province, Jinan 250014, China. rock0515@163.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of New Drug & Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, China. hope1126@hotmail.com.
authors.forename: Zhen-Liang
authors.initials: ZL
authors.lastname: Sun
authors.affiliation: Biology Institute of Shandong Academy of Sciences/Key Laboratory for Applied Microbiology of Shandong Province, Jinan 250014, China. jiaar@sdas.org.
authors.forename: Ai-Rong
authors.initials: AR
authors.lastname: Jia
authors.affiliation: Biology Institute of Shandong Academy of Sciences/Key Laboratory for Applied Microbiology of Shandong Province, Jinan 250014, China. hand-user@163.com.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Shi
authors.affiliation: Fengxian Hospital Affiliated to Southern Medical University, 6600 NanFeng Road, Shanghai 201499, China. fengxianhospital@163.com.
authors.forename: Rui-Hong
authors.initials: RH
authors.lastname: Li
authors.affiliation: State Key Laboratory of New Drug & Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, China. peimingy@163.com.
authors.forename: Pei-Ming
authors.initials: PM
authors.lastname: Yang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004264:DNA Topoisomerases, Type I / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D017665:Hydroxyl Radical / Q000737:chemistry; D036142:Mentha piperita / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013481:Superoxides / Q000737:chemistry; D013696:Temperature
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D011134:Polysaccharides; D013481:Superoxides; D017665:Hydroxyl Radical; D004264:DNA Topoisomerases, Type I