title: [Level of DNA fragmentation in human sperm cells in varicocele and prostatitis].
abstract: Varicocele and prostatitis are the most common andrological diseases, which may be accompanied by a decrease in the production of sperm cells, the deterioration of their quality and increased risk of infertility. This work was aimed to the evaluation of sperm DNA fragmentation index (DFI) and main indices of sperm fertility (concentration, motility and morphology), and the relationship between these parameters in the men of active reproductive age suffering from prostatitis or varicocele. Assessment of sperm DNA fragmentation was performed by SCSA (sperm chromatin structure assay) using flow cytometry; sperm parameters were evaluated according to WHO recommendations. It was shown that men with prostatitis (n = 9) and varicocele (n = 22) had significantly higher DFI compared with men in the control group (n = 22). Negative influence of these diseases on the concentration and the percentage of motile sperm cells in the ejaculate was revealed. These data suggest that the deterioration in the quality of semen in varicocele and prostatitis may be caused not only by pathospermia, but also, at least partially, by violation of the integrity of the sperm DNA. Evaluation of sperm DNA fragmentation can be recommended for use in laboratory diagnostics for prediction of fertility in infertile men.
authors.forename: L V
authors.initials: LV
authors.lastname: Osadchuk
authors.forename: A A
authors.initials: AA
authors.lastname: Erkovich
authors.forename: D A
authors.initials: DA
authors.lastname: Tataru
authors.forename: E V
authors.initials: EV
authors.lastname: Markova
authors.forename: A V
authors.initials: AV
authors.lastname: Svetlakov
mesh_terms: D000328:Adult; D053938:DNA Fragmentation; D006801:Humans; D007248:Infertility, Male / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D011472:Prostatitis / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*; D014646:Varicocele / Q000150:complications / Q000378:metabolism* / Q000473:pathology