title: Brij detergents reveal new aspects of membrane microdomain in erythrocytes.
abstract: Membrane microdomains enriched in cholesterol, sphingolipids (rafts), and specific proteins are involved in important physiological functions. However their structure, size and stability are still controversial. Given that detergent-resistant membranes (DRMs) are in the liquid-ordered state and are rich in raft-like components, they might correspond to rafts at least to some extent. Here we monitor the lateral order of biological membranes by characterizing DRMs from erythrocytes obtained with Brij-98, Brij-58, and TX-100 at 4 °C and 37 °C. All DRMs were enriched in cholesterol and contained the raft markers flotillin-2 and stomatin. However, sphingomyelin (SM) was only found to be enriched in TX-100-DRMs - a detergent that preferentially solubilizes the membrane inner leaflet - while Band 3 was present solely in Brij-DRMs. Electron paramagnetic resonance spectra showed that the acyl chain packing of Brij-DRMs was lower than TX-100-DRMs, providing evidence of their diverse lipid composition. Fatty acid analysis revealed that the SM fraction of the DRMs was enriched in lignoceric acid, which should specifically contribute to the resistance of SM to detergents. These results indicate that lipids from the outer leaflet, particularly SM, are essential for the formation of the liquid-ordered phase of DRMs. At last, the differential solubilization process induced by Brij-98 and TX-100 was monitored using giant unilamellar vesicles. This study suggests that Brij and TX-100-DRMs reflect different degrees of lateral order of the membrane microdomains. Additionally, Brij DRMs are composed by both inner and outer leaflet components, making them more physiologically relevant than TX-100-DRMs to the studies of membrane rafts.
authors.affiliation: Departamento de Bioquímica e Biologia Tecidual, Instituto de Biologia, Universidade Estadual de Campinas (Unicamp) , Campinas , Brazil.
authors.forename: Bruna Renata
authors.initials: BR
authors.lastname: Casadei
authors.forename: Patrícia
authors.initials: P
authors.lastname: De Oliveira Carvalho
authors.forename: Karin A
authors.initials: KA
authors.lastname: Riske
authors.forename: Raquel De Melo
authors.initials: Rde M
authors.lastname: Barbosa
authors.forename: Eneida
authors.initials: E
authors.lastname: De Paula
authors.forename: Cleyton Crepaldi
authors.initials: CC
authors.lastname: Domingues
mesh_terms: D002784:Cholesterol / Q000737:chemistry; D003902:Detergents / Q000737:chemistry*; D004912:Erythrocytes / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry; D021962:Membrane Microdomains / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D003902:Detergents; D005227:Fatty Acids; D008051:Lipid Bilayers; D008565:Membrane Proteins; D010938:Plant Oils; C036860:STOM protein, human; C106354:flotillins; D011092:Polyethylene Glycols; C033084:polyethylene glycol oleyl ether; D002784:Cholesterol; C010210:lignoceric acid
keywords: Detergent; erythrocyte; lipid bilayer; lipid rafts