title: Polyethylenimine derivate conjugated with RGD-TAT-NLS as a novel gene vector.
abstract: To solve the contradiction between the cell toxicity and transfection efficiency of polyethylenimine (PEI) derivate in non-viral gene therapy, a novel gene vector, P123-PEI-R18 was synthesized by using biodegradable PEI derivate conjugated with trifunctional peptide RGD-TAT-NLS. The particle size of P123-PEI-R18/DNA was around 100-250 nm. The gene vector could condense DNA at the weight ratio of 2 and protect plasmid DNA from being dissolved in the blood circulation. Importantly, the complexes exhibited lower cell toxicity and higher transfection efficiency contrasted with PEI 25 kDa in vitro. P123-PEI-R18 holds high potential as a safe and efficient gene vector.
authors.affiliation: Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Wenfang
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Kehai
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Shunsheng
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Man Man
authors.initials: MM
authors.lastname: Zhu
authors.affiliation: Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Lv
authors.affiliation: Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Mao
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007097:Imines / Q000737:chemistry; D047908:Intracellular Signaling Peptides and Proteins; D008545:Melanoma / Q000235:genetics*; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D057054:Molecular Imaging / Q000379:methods*; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010455:Peptides / Q000737:chemistry / Q000493:pharmacokinetics*; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics*; D011060:Poloxalene / Q000737:chemistry; D011095:Polyethylenes / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D014162:Transfection / Q000379:methods*
chemical_list: D007097:Imines; D047908:Intracellular Signaling Peptides and Proteins; D053769:Nanocapsules; D010455:Peptides; D011095:Polyethylenes; D011108:Polymers; C527524:R18 peptide; C505405:poly(ethylene imine); C464484:pluronic block copolymer P123; D011060:Poloxalene
keywords: Non-viral gene vector; P123; avß3; nuclear localization signal; polyethylenimine