title: Enhanced production of botrallin and TMC-264 with in situ macroporous resin adsorption in mycelial liquid culture of the endophytic fungus Hyalodendriella sp. Ponipodef12.
abstract: Hyalodendriella sp. Ponipodef12, an endophytic fungus from the hybrid "Neva" of Populus deltoides × P. nigra, is a high producer of the bioactive dibenzo-α-pyrones botrallin and TMC-264. However, both the botrallin and TMC-264 produced by Hyalodendriella sp. Ponipodef12 were retained as both intracellular and extracellular products. The aim of this study was to evaluate an in situ macroporous resin adsorption for enhancement of botrallin and TMC-264 production in mycelial liquid culture of Hyalodendriella sp. Ponipodef12. Production of botrallin and TMC-264 was most effectively enhanced by macroporous resin DM-301 among the thirteen nonionic macroporous resins tested. The highest botrallin yield (51.47 mg/L, which was 2.29-fold higher than the control at 22.49 mg/L) was obtained by adding resin DM-301 at 4.38% (g/mL) to the culture broth on day 24 and allowing a period of 4 days for adsorption. The highest TMC-264 yield reached 47.74 mg/L, which was 11.76-fold higher than that of the control (4.06 mg/L), and was achieved by adding DM-301 resin at 4.38% (w/v) in the culture broth on day 24 and allowing a period of 6 days for adsorption. The results show that in situ resin adsorption is an effective strategy for enhancing production of botrallin and TMC-264, and also for facilitating their recovery from mycelial liquid culture of Hyalodendriella sp. Ponipodef12.
authors.affiliation: MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. luohaiyu69@163.com.
authors.forename: Haiyu
authors.initials: H
authors.lastname: Luo
authors.affiliation: MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. luohaiyu69@163.com.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Liu
authors.affiliation: MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. caoyuheng1990@163.com.
authors.forename: Yuheng
authors.initials: Y
authors.lastname: Cao
authors.affiliation: MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. cauxudan@163.com.
authors.forename: Dan
authors.initials: D
authors.lastname: Xu
authors.affiliation: MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. maoziling2011@163.com.
authors.forename: Ziling
authors.initials: Z
authors.lastname: Mao
authors.affiliation: MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. muyan01987@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Mou
authors.affiliation: MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. mengjiajiax@163.com.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Meng
authors.affiliation: MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. dwlai@cau.edu.cn.
authors.forename: Daowan
authors.initials: D
authors.lastname: Lai
authors.affiliation: Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China. liuyang01@caas.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. lgzhou@cau.edu.cn.
authors.forename: Ligang
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000327:Adsorption; D001203:Ascomycota / Q000254:growth & development / Q000378:metabolism; D019149:Bioreactors; D046508:Culture Techniques; D006575:Heterocyclic Compounds, 3-Ring / Q000302:isolation & purification* / Q000378:metabolism; D025282:Mycelium / Q000254:growth & development / Q000378:metabolism; D016062:Porosity; D011753:Pyrones / Q000302:isolation & purification* / Q000378:metabolism; D012117:Resins, Synthetic / Q000737:chemistry
chemical_list: D006575:Heterocyclic Compounds, 3-Ring; D011753:Pyrones; D012117:Resins, Synthetic; C476504:TMC-264; C000598261:botrallin