title: Characterization of one novel cry8 gene from Bacillus thuringiensis strain Q52-7.
abstract: Bacillus thuringiensis (Bt) is the most widely used insecticidal microbe due to its specific toxicity and safe use with respect to animals and the environment. In this study, we isolated Bt strain Q52-7 from a soil sample collected in the Qian Shan District, Liao Ning Province, China. We observed that the Q52-7 strain produced spherical crystals. The Bt Q52-7 strain had high toxicity against Asian Cockchafer (Holotrichia parallela), exhibiting an LC50 of 3.80 × 10(9) cfu/g, but is not toxic for Anomala corpulenta Motschulsky and Holotrichia oblita. Using general cry8 primers, we amplified a 1.3 kb fragment with the polymerase chain reaction. Specific primers were designed for the amplified fragment to clone the full-length coding region. A novel gene, cry8Na1, had 69 % sequence similarity with cry8Ca1. cry8Na1 gene was successfully expressed in the HD-73(-) acrystalliferous mutant of Bt subsp. Kurstaki HD-73. Bioassays demonstrated that the Cry8Na1 protein is highly toxic for the H. parallela, with a 50 % lethal concentration of 8.18 × 10(10) colony forming units per gram.
authors.affiliation: Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.forename: Rongmei
authors.initials: R
authors.lastname: Liu
authors.forename: Changlong
authors.initials: C
authors.lastname: Shu
authors.forename: Qingli
authors.initials: Q
authors.lastname: Zhang
authors.forename: Shiyuan
authors.initials: S
authors.lastname: Zhao
authors.forename: Gaoxiang
authors.initials: G
authors.lastname: Shao
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zhang
authors.forename: Jiguo
authors.initials: J
authors.lastname: Gao
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000166:cytology / Q000235:genetics* / Q000302:isolation & purification; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000633:toxicity*; D001681:Biological Assay; D002681:China; D003001:Cloning, Molecular; D016000:Cluster Analysis; D001517:Coleoptera / Q000187:drug effects; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004731:Endotoxins / Q000235:genetics* / Q000633:toxicity*; D015870:Gene Expression; D006460:Hemolysin Proteins / Q000235:genetics* / Q000633:toxicity*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012988:Soil Microbiology; D016019:Survival Analysis
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis