title: Influence of the nanostructure of F-doped TiO2 films on osteoblast growth and function.
abstract: The aim of this study was to evaluate the proliferation and mineralization ability of mouse osteoblastic MC3T3-E1 cells on F-containing TiO2 films with different morphology and nanostructure that previously confirmed antibacterial properties. F-containing TiO2 films were fabricated by anodizing Ti-6Al-4V alloy ELI -grade 23. By using a mixture of H2SO4/HF acid at 20 V for 5 and 60 min, a TiO2 film grows with nanoporous (NP) and nanotubular (NT) features, characterized with a pore diameter of 20 and 100 nm, respectively. Fluoride-TiO2 barrier films (FBL) were produced in 1M NH4H2PO4/0.15M NH4F solution at constant voltage controlled at 20 V for 120 min. The amount of F incorporated in the nanostructured oxide films was 6 at % and of 4 at %, for the NP and NT, respectively, while for the FBL film was 12 at %. MC3T3-E1 cells exhibited different behavior when seeded and grown onto these surfaces. Thus, F-doped TiO2 films with NP structures increased proliferation as well as osteogenic gene expression and the mineralization capacity of these osteoblastic cells. These results confirm that anodizing process is suitable to fabricate multifunctional surfaces on Ti-6Al-4V alloy with improved not only antibacterial but also osteogenic properties useful for bone fixation of prosthetic devices
authors.affiliation: Grupo de Investigación de Cirugía OsteoArticular, Instituto de Investigación Hospital Universitario La Paz (IdiPAZ), 28046 Madrid, Spain; Laboratorio de Metabolismo Mineral y Óseo, Instituto de Investigación Sanitaria (IIS)-Fundación Jiménez Díaz, UAM, 28040 Madrid, Spain, and RETICEF, Instituto de Salud Carlos III, 28029 Madrid, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Lozano
authors.forename: Juan M
authors.initials: JM
authors.lastname: Hernández-López
authors.forename: Pedro
authors.initials: P
authors.lastname: Esbrit
authors.forename: Maria A
authors.initials: MA
authors.lastname: Arenas
authors.forename: Enrique
authors.initials: E
authors.lastname: Gómez-Barrena
authors.forename: Juan
authors.initials: J
authors.lastname: de Damborenea
authors.forename: Jaime
authors.initials: J
authors.lastname: Esteban
authors.forename: Concepción
authors.initials: C
authors.lastname: Pérez-Jorge
authors.forename: Ramón
authors.initials: R
authors.lastname: Pérez-Tanoira
authors.forename: Ana
authors.initials: A
authors.lastname: Conde
mesh_terms: D000497:Alloys; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005459:Fluorides / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D014025:Titanium / Q000494:pharmacology*
chemical_list: D000497:Alloys; C031462:titanium alloy (TiAl6V4); C009495:titanium dioxide; D014025:Titanium; D005459:Fluorides
keywords: Ti-6Al-4V alloy; anodizing; osteoblast growth and function; surface functionalization