title: Resveratrol affects histone 3 lysine 27 methylation of vessels and blood biomarkers in DOCA salt-induced hypertension.
abstract: Hypertension is a risk factor for the cardiovascular diseases. Although, several drugs are used to treat hypertension, the success of the antihypertensive therapy is limited. Resveratrol decreases blood pressure in animal models of hypertension. This study researched the mechanisms behind the effects of resveratrol on hypertension. Hypertension was induced by using the deoxycorticosterone acetate (DOCA)-induced (15 mg/kg twice per week, subcutaneously) salt-sensitive hypertension model of Wistar rats. Hypertension caused a decrease in endothelium-dependent relaxations of the isolated thoracic aorta. Resveratrol treatment (50 mg/l in drinking water) prevented DOCA salt-induced hypertension, but did not improve endothelial dysfunction. Plasma nitric oxide (NO), asymmetric dimethylarginine (ADMA), total antioxidant capacity (TAC) and hydrogen sulfide (H2S) levels were not changed by DOCA salt application. However, treatment of resveratrol significantly decreased ADMA and increased TAC and H2S levels. NO level in circulation was not significantly changed by resveratrol. DOCA salt application and resveratrol treatment also caused an alteration in the epigenetic modification of vessels. Staining pattern of histone 3 lysine 27 methylation (H3K27me3) in the aorta and renal artery sections was changed. These results show that preventive effect of resveratrol on DOCA salt-induced hypertension might due to its action on the production of some blood biomarkers and the epigenetic modification of vessels that would focus upon new aspect of hypertension prevention and treatment.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, Gazi University, Etiler, 06330, Ankara, Turkey.
authors.forename: Sevtap
authors.initials: S
authors.lastname: Han
authors.forename: Mecit Orhan
authors.initials: MO
authors.lastname: Uludag
authors.forename: Suzan Emel
authors.initials: SE
authors.lastname: Usanmaz
authors.forename: Fatma
authors.initials: F
authors.lastname: Ayaloglu-Butun
authors.forename: Kamil Can
authors.initials: KC
authors.lastname: Akcali
authors.forename: Emine
authors.initials: E
authors.lastname: Demirel-Yilmaz
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001120:Arginine / Q000031:analogs & derivatives / Q000097:blood; D015415:Biomarkers / Q000097:blood*; D001794:Blood Pressure / Q000187:drug effects; D001808:Blood Vessels / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D064791:Desoxycorticosterone Acetate; D004730:Endothelium, Vascular / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D005455:Fluorescent Antibody Technique; D006657:Histones / Q000378:metabolism*; D006862:Hydrogen Sulfide / Q000097:blood; D006973:Hypertension / Q000097:blood* / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D008239:Lysine / Q000378:metabolism*; D008297:Male; D008745:Methylation / Q000187:drug effects; D009573:Nitrites / Q000097:blood; D017208:Rats, Wistar; D012077:Renal Artery / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013599:Systole / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D006657:Histones; D009573:Nitrites; D013267:Stilbenes; C018524:N,N-dimethylarginine; D064791:Desoxycorticosterone Acetate; D001120:Arginine; D008239:Lysine; D000077185:Resveratrol; D006862:Hydrogen Sulfide