title: Microchip-based human serum atherogenic lipoprotein profile analysis.
abstract: Owing to the mounting evidence of serum lipid changes in atherosclerosis, there has been increasing interest in developing new methods for analyzing atherogenic lipoprotein profiles. The separation of lipoprotein and lipoprotein subclasses has been demonstrated using a microchip capillary electrophoresis (CE) system [Chromatographia 74 (2011) 799-805]. In contrast to this previous study, the current report demonstrates that sdLDL peak efficiencies can be improved dramatically by adding gold nanoparticles (AuNPs) to the sample. Moreover, NBD C6-ceramide was identified as a satisfactory dye for specific labeling and quantitation of individual serum lipoproteins. The accuracy of the method was evaluated by comparison with ultracentrifuge separated small, dense, low-density lipoprotein (sdLDL). A high correlation was observed between these two methods for sdLDL cholesterol. Lipid levels were investigated between atherosclerotic patients and healthy controls. The variation of serum atherogenic lipoprotein profiles for atherosclerotic patients pre- and post-treatment was assessed by microchip CE. This method has potential for the rapid and sensitive detection of different lipoprotein classes as well as their subclasses and, therefore, is suitable for routine clinical applications. Microchip-based atherogenic lipoprotein profile assays will greatly improve the analysis of risk factors in atherosclerosis and will provide useful information for monitoring the effect of therapies on atherosclerotic disease.
authors.affiliation: Department of Laboratory Medicine and Central Laboratory, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: Biomedical Research Institute, Shenzhen PKU-HKUST Medical Center, Shenzhen, Guangdong 518036, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Biomedical Research Institute, Shenzhen PKU-HKUST Medical Center, Shenzhen, Guangdong 518036, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wan
authors.affiliation: Department of Laboratory Medicine, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China; Department of Laboratory Medicine, Shanghai Skin Disease Hospital, Shanghai 200071, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Dermatology, Shenzhen Hospital, Peking University, Shenzhen, Guangdong 518036, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.affiliation: Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai 200050, China.
authors.forename: Qinghui
authors.initials: Q
authors.lastname: Jin
authors.affiliation: Department of Laboratory Medicine and Central Laboratory, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China. Electronic address: guanming88@yahoo.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Guan
mesh_terms: D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives / Q000737:chemistry; D000328:Adult; D000368:Aged; D002340:Carotid Artery Diseases / Q000097:blood*; D016022:Case-Control Studies; D002518:Ceramides / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000379:methods*; D005260:Female; D006046:Gold / Q000737:chemistry; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood*; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry; D008875:Middle Aged
chemical_list: D002518:Ceramides; D008074:Lipoproteins; D006046:Gold; C037974:N-(7-(4-nitrobenzo-2-oxa-1,3-diazole))-6-aminocaproyl sphingosine; D009327:4-Chloro-7-nitrobenzofurazan
keywords: Atherogenic lipoprotein profile; Gold nanoparticles; Microchip; NBD C(6)-ceramide