title: Innate lymphoid cells regulate intestinal epithelial cell glycosylation.
abstract: Fucosylation of intestinal epithelial cells, catalyzed by fucosyltransferase 2 (Fut2), is a major glycosylation mechanism of host-microbiota symbiosis. Commensal bacteria induce epithelial fucosylation, and epithelial fucose is used as a dietary carbohydrate by many of these bacteria. However, the molecular and cellular mechanisms that regulate the induction of epithelial fucosylation are unknown. Here, we show that type 3 innate lymphoid cells (ILC3) induced intestinal epithelial Fut2 expression and fucosylation in mice. This induction required the cytokines interleukin-22 and lymphotoxin in a commensal bacteria-dependent and -independent manner, respectively. Disruption of intestinal fucosylation led to increased susceptibility to infection by Salmonella typhimurium. Our data reveal a role for ILC3 in shaping the gut microenvironment through the regulation of epithelial glycosylation.
authors.affiliation: Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan. Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Saitama 332-0012, Japan. Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba 305-0074, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Goto
authors.affiliation: Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan. Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba 305-0074, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Obata
authors.affiliation: Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan. Laboratory of Vaccine Materials, National Institute of Biomedical Innovation, Osaka 567-0085, Japan. Division of Mucosal Immunology, International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Kunisawa
authors.affiliation: Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan. Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Saitama 332-0012, Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Sato
authors.affiliation: Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY 10032, USA.
authors.forename: Ivaylo I
authors.initials: II
authors.lastname: Ivanov
authors.affiliation: Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.
authors.forename: Aayam
authors.initials: A
authors.lastname: Lamichhane
authors.affiliation: Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan. Nippon Institute for Biological Science, Tokyo 198-0024, Japan.
authors.forename: Natsumi
authors.initials: N
authors.lastname: Takeyama
authors.affiliation: Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Kamioka
authors.affiliation: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba 305-0074, Japan.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sakamoto
authors.affiliation: Yakult Central Institute, Tokyo 186-8650, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Matsuki
authors.affiliation: Yakult Central Institute, Tokyo 186-8650, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Setoyama
authors.affiliation: Yakult Central Institute, Tokyo 186-8650, Japan.
authors.forename: Akemi
authors.initials: A
authors.lastname: Imaoka
authors.affiliation: Division of Innate Immune Regulation, International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan. Department of Mucosal Immunology, School of Medicine, Chiba University, 1-8-1 Inohana, Chuou-ku, Chiba, 260-8670, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Uematsu
authors.affiliation: Laboratory of Host Defense, WPI Immunology Frontier Research Center, Osaka University, Osaka 565-0871, Japan.
authors.forename: Shizuo
authors.initials: S
authors.lastname: Akira
authors.affiliation: Department of Obstetrics and Gynecology, Cellular and Molecular Biology Program, University of Michigan Medical Center, Ann Arbor, MI 48109-5617, USA.
authors.forename: Steven E
authors.initials: SE
authors.lastname: Domino
authors.affiliation: Institute of Experimental Immunology, University of Zürich, Winterthurerstrasse 190, Zürich CH-8057, Switzerland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Kulig
authors.affiliation: Institute of Experimental Immunology, University of Zürich, Winterthurerstrasse 190, Zürich CH-8057, Switzerland.
authors.forename: Burkhard
authors.initials: B
authors.lastname: Becher
authors.affiliation: Ludwig Institute for Cancer Research and Université Catholique de Louvain, Brussels B-1200, Belgium.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Renauld
authors.affiliation: Nippon Institute for Biological Science, Tokyo 198-0024, Japan. Division of Bacterial Infection, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan. Medical Mycology Research Center, Chiba University, Chiba 260-8673, Japan.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Sasakawa
authors.affiliation: Yakult Central Institute, Tokyo 186-8650, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Umesaki
authors.affiliation: Benno Laboratory, Innovation Center, RIKEN, Wako, Saitama 351-0198, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Benno
authors.affiliation: Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan. Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Saitama 332-0012, Japan. Division of Mucosal Immunology, International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyono
mesh_terms: D000818:Animals; D001483:Base Sequence; D004195:Disease Models, Animal; D005643:Fucose / Q000378:metabolism*; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism; D005856:Germ-Free Life; D006031:Glycosylation; D020397:Goblet Cells / Q000201:enzymology / Q000276:immunology / Q000382:microbiology; D007082:Ileum / Q000201:enzymology / Q000276:immunology / Q000382:microbiology; D007113:Immunity, Innate; D007378:Interleukins / Q000276:immunology; D007413:Intestinal Mucosa / Q000201:enzymology / Q000276:immunology* / Q000382:microbiology; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D064307:Microbiota / Q000276:immunology*; D008969:Molecular Sequence Data; D019879:Paneth Cells / Q000201:enzymology / Q000276:immunology / Q000382:microbiology; D012480:Salmonella Infections / Q000276:immunology* / Q000382:microbiology; D012486:Salmonella typhimurium; D000097043:Interleukin-22; D000097631:Galactoside 2-alpha-L-fucosyltransferase
chemical_list: D005643:Fucose; D005647:Fucosyltransferases; D007378:Interleukins; D000097043:Interleukin-22; D000097631:Galactoside 2-alpha-L-fucosyltransferase