title: pH-sensitive chitosan/alginate core-shell nanoparticles for efficient and safe oral insulin delivery.
abstract: Chitosan-alginate (CS/ALG) nanoparticles were prepared by formation of an ionotropic pre-gelation of an alginate (ALG) core entrapping insulin, followed by chitosan (CS) polyelectrolyte complexation, for successful oral insulin administration. Mild preparation process without harsh chemicals is aimed at improving insulin bio-efficiency in in vivo model. The nanoparticles showed an average particle size of 100-200 nm in dynamic light scattering (DLS), with almost spherical or sub-spherical shape and ∼ 85% of insulin encapsulation. Again, retention of almost entire amount of encapsulated insulin in simulated gastric buffer followed by its sustained release in simulated intestinal condition proved its pH sensitivity in in vitro release studies. Significant hypoglycemic effects with improved insulin-relative bioavailability (∼ 8.11%) in in vivo model revealed the efficacy of these core-shell nanoparticles of CS/ALG as an oral insulin carrier. No systemic toxicity was found after its peroral treatment, suggesting these core-shell nanoparticles as a promising device for potential oral insulin delivery.
authors.affiliation: Department of Polymer Science and Technology, University of Calcutta, 92, A.P.C. Road, Kolkata 700009, India.
authors.forename: Piyasi
authors.initials: P
authors.lastname: Mukhopadhyay
authors.affiliation: Department of Polymer Science and Technology, University of Calcutta, 92, A.P.C. Road, Kolkata 700009, India.
authors.forename: Souma
authors.initials: S
authors.lastname: Chakraborty
authors.affiliation: Department of Physiology, University of Calcutta, 92, A.P.C. Road, Kolkata 700009, India.
authors.forename: Sourav
authors.initials: S
authors.lastname: Bhattacharya
authors.affiliation: Department of Physiology, University of Calcutta, 92, A.P.C. Road, Kolkata 700009, India.
authors.forename: Roshnara
authors.initials: R
authors.lastname: Mishra
authors.affiliation: Department of Polymer Science and Technology, University of Calcutta, 92, A.P.C. Road, Kolkata 700009, India. Electronic address: ppk923@yahoo.com.
authors.forename: P P
authors.initials: PP
authors.lastname: Kundu
mesh_terms: D000284:Administration, Oral; D000464:Alginates / Q000008:administration & dosage* / Q000737:chemistry; D000818:Animals; D001682:Biological Availability; D048271:Chitosan / Q000008:administration & dosage* / Q000737:chemistry; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D016503:Drug Delivery Systems; D020723:Glucuronic Acid / Q000008:administration & dosage / Q000737:chemistry; D006603:Hexuronic Acids / Q000008:administration & dosage / Q000737:chemistry; D007328:Insulin / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D007328:Insulin; D020723:Glucuronic Acid; D048271:Chitosan
keywords: Bioavailability; Chitosan/alginate pH-sensitive nanoparticles; Oral insulin delivery