title: [How to select the spermatozoon in ICSI?].
abstract: The selection of the individual spermatozoon in ICSI is routinely performed by the observation of its motility and morphology. However, in case of severe oligoasthenozoospermia or non-obstructive azoospermia needing the use of testicular sperm, other methods are necessary to help the embryologist making this choice. According to some authors, sperm processing before ICSI seems to limit the DNA fragmentation index, and in this way improve ICSI outcomes. Moreover, IMSI is potentially a good option in some specific indications such as severe teratozoospermia, or repeated ICSI failures. Other methods based on sperm structure, as sperm head birefringence observation, or based on its function, like the hyaluronic acid or zona pellucida binding capacity, could be of interest, but still need to be confirmed. Finally, in case of akinetozoospermia, the use of functional tests, such as pentoxifylline test, HOS-test, or to a lesser extent laser touch, makes the selection of viable spermatozoa easier. Nevertheless, studies on larger series have to be conducted to evaluate and precise the interest of each of these methods and their indications, before considering an application on larger scale.
authors.affiliation: Service d'histologie-embryologie-cytogénétique-CECOS, centre hospitalier universitaire Jean-Verdier, Assistance Publique-Hôpitaux de Paris, avenue du 14-Juillet, 93140 Bondy, France.
authors.forename: C
authors.initials: C
authors.lastname: Herbemont
authors.affiliation: Service d'histologie-embryologie-cytogénétique-CECOS, centre hospitalier universitaire Jean-Verdier, Assistance Publique-Hôpitaux de Paris, avenue du 14-Juillet, 93140 Bondy, France. Electronic address: christophe.sifer@jvr.aphp.fr.
authors.forename: C
authors.initials: C
authors.lastname: Sifer
mesh_terms: D005260:Female; D006801:Humans; D007248:Infertility, Male; D008297:Male; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013081:Sperm Motility; D013094:Spermatozoa / Q000002:abnormalities / Q000473:pathology* / Q000502:physiology*; D013737:Testis / Q000166:cytology
keywords: Fonction; Function; ICSI; Sperm selection; Structure; Sélection du spermatozoïde