title: The relationships between type 2 diabetic retinopathy and VEGF-634G/C and VEGF-460C/T polymorphisms in Han Chinese subjects.
abstract: OBJECTIVE
  To investigate how VEGF-634G/C and VEGF-460C/T SNPs are related to diabetic retinopathy (DR) in Han Chinese subjects from the Shijiazhuang region of China.
  
  
  METHODS
  Totally 376 DM cases were divided into non-proliferative diabetic retinopathy (NPDR) group (n=124), proliferative diabetic retinopathy (PDR) group (n=108), and diabetes without retinopathy (DWR) group (n=144). PCR/LDRwas utilised to detect and assess the genotypes and allele distribution frequencies at the VEGF-634G/C and VEGF-460C/T loci in each group.
  
  
  RESULTS
  The differences between NPDR, PDR and DWR groups were not significant in genotypes and allele distribution frequencies at VEGF-634G/C locus (P>0.05). But there were significant differences between NPDR and DWR groups in genotypes (P=0.013) and allele distribution frequencies (P=0.002) at VEGF-460C/T locus, at which CT+CC genotypes were associated with a reduced risk of developing NPDR. There were no significant differences in genotypes (P=0.759) or allele distribution frequencies (P=0.433) at VEGF-460C/T locus between PDR and DWR groups.
  
  
  CONCLUSIONS
  Among Chinese Han individuals with type-2 DM, polymorphism -634G/C of the VEGF gene was not correlated with NPDR or PDR; however, polymorphism-460C/T of the VEGF gene was correlated with NPDR, and C allele was associated with lower NPDR risk than T allele.
authors.affiliation: Department of Ophthalmology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China 050011.
authors.forename: Yazhen
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Ophthalmology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China 050011.
authors.forename: Zhuan
authors.initials: Z
authors.lastname: Wen
authors.affiliation: Department of Ophthalmology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China 050011. Electronic address: Yongqingguan@hotmail.com.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Guan
authors.affiliation: Shijiazhuang Aier Hospital Department of Ophthalmology, Hebei Province, China 050000.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Ophthalmology, the People's Hospital of Binzhou, Shandong Province, China 256600.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Ophthalmology, Affiliated Hospital of Chengde Medical College, Hebei Province, China 067000.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Fan
authors.affiliation: Department of Ophthalmology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China 050011.
authors.forename: Xinzhi
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Ophthalmology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China 050011.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000208:ethnology / Q000235:genetics*; D003930:Diabetic Retinopathy / Q000208:ethnology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: Chinese population; Diabetic retinopathy; Genetic association; Single nucleotide polymorphism; Vascular endothelial growth factor