title: Proteomic analysis of keratitis-associated Pseudomonas aeruginosa.
abstract: OBJECTIVE
  To compare the proteomic profile of a clinical isolate of Pseudomonas aeruginosa (P. aeruginosa) obtained from an infected cornea of a contact lens wearer and the laboratory strain P. aeruginosa ATCC 10145.
  
  
  METHODS
  Antibiotic sensitivity, motility, biofilm formation, and virulence tests were performed using standard methods. Whole protein lysates were analyzed with liquid chromatography/ tandem mass spectrometry (LC-MS/MS) in triplicate, and relative protein abundances were determined with spectral counting. The G test followed by a post hoc Holm-Sidak adjustment was used for the statistical analyses to determine significance in the differential expression of proteins between the two strains.
  
  
  RESULTS
  A total of 687 proteins were detected. One-hundred thirty-three (133) proteins were significantly different between the two strains. Among these, 13 were upregulated, and 16 were downregulated in the clinical strain compared to ATCC 10145, whereas 57 were detected only in the clinical strain. The upregulated proteins are associated with virulence and pathogenicity.
  
  
  CONCLUSIONS
  Proteins detected at higher levels in the clinical strain of P. aeruginosa were proteins known to be virulence factors. These results confirm that the keratitis-associated P. aeruginosa strain is pathogenic and expresses a higher number of virulence factors compared to the laboratory strain ATCC 10145. Identification of the protein profile of the corneal strain of P. aeruginosa in this study will aid in elucidating novel intervention strategies for reducing the burden of P. aeruginosa infection in keratitis.
authors.affiliation: Summa Health System, Akron, OH.
authors.forename: Abby
authors.initials: A
authors.lastname: Sewell
authors.affiliation: Summa Health System, Akron, OH.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Dunmire
authors.affiliation: Summa Health System, Akron, OH.
authors.forename: Michael
authors.initials: M
authors.lastname: Wehmann
authors.affiliation: Summa Health System, Akron, OH.
authors.forename: Theresa
authors.initials: T
authors.lastname: Rowe
authors.affiliation: Summa Health System, Akron, OH.
authors.forename: Rachida
authors.initials: R
authors.lastname: Bouhenni
mesh_terms: D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D003261:Contact Lenses / Q000009:adverse effects / Q000382:microbiology; D003320:Corneal Ulcer / Q000382:microbiology*; D015818:Eye Infections, Bacterial / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D020543:Proteome / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000378:metabolism* / Q000472:pathogenicity*; D013045:Species Specificity; D014774:Virulence; D037521:Virulence Factors / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D037521:Virulence Factors