title: Arm-specific telomere dynamics of each individual chromosome in induced pluripotent stem cells revealed by quantitative fluorescence in situ hybridization.
abstract: We have reported that telomere fluorescence units (TFUs) of established induced pluripotent stem cells (iPSCs) derived from human amnion (hAM933) and fetal lung fibroblasts (MRC-5) were significantly longer than those of the parental cells, and that the telomere extension rates varied quite significantly among clones without chromosomal instability, although the telomeres of other iPSCs derived from MRC-5 became shorter as the number of passages increased along with chromosomal abnormalities from an early stage. In the present study we attempted to clarify telomere dynamics in each individual chromosomal arm of parental cells and their derived clonal human iPSCs at different numbers of passages using quantitative fluorescence in situ hybridization (Q-FISH). Although no speciﬁc arm of any particular chromosome appeared to be consistently shorter or longer than most of the other chromosomes in any of the cell strains, telomere elongation in each chromosome of an iPSC appeared to be random and stochastic. However, in terms of the whole genome of any specific cell, the telomeres showed overall elongation associated with iPSC generation. We have thus demonstrated the specific telomere dynamics of each individual chromosomal arm in iPSCs derived from parental cells, and in the parental cells themselves, using Q-FISH.
authors.affiliation: Research Team for Geriatric Pathology, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan; Department of Judotherapy, Faculty of Health Sciences, Tokyo Ariake University of Medical and Health Sciences, Tokyo 135-0063, Japan. Electronic address: terai@tau.ac.jp.
authors.forename: Masanori
authors.initials: M
authors.lastname: Terai
authors.affiliation: Research Team for Geriatric Pathology, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan.
authors.forename: Naotaka
authors.initials: N
authors.lastname: Izumiyama-Shimomura
authors.affiliation: Research Team for Geriatric Pathology, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Aida
authors.affiliation: Research Team for Geriatric Pathology, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan.
authors.forename: Naoshi
authors.initials: N
authors.lastname: Ishikawa
authors.affiliation: Department of Analytical Chemistry, Yokohama College of Pharmacy, Yokohama 245-0066, Japan.
authors.forename: Mie
authors.initials: M
authors.lastname: Kuroiwa
authors.affiliation: Department of Pathology, Tokyo Metropolitan Geriatric Hospital, Tokyo 173-0015, Japan.
authors.forename: Tomio
authors.initials: T
authors.lastname: Arai
authors.affiliation: Research Team for Vascular Medicine, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan; Department of Reproductive Biology, National Research Institute for Child Health and Development, Tokyo 157-8535, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Toyoda
authors.affiliation: Research Team for Geriatric Pathology, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Nakamura
authors.affiliation: Research Team for Geriatric Pathology, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan. Electronic address: Takubo@tmig.or.jp.
authors.forename: Kaiyo
authors.initials: K
authors.lastname: Takubo
mesh_terms: D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000235:genetics / Q000648:ultrastructure*; D004171:Diploidy; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D057026:Induced Pluripotent Stem Cells / Q000648:ultrastructure*; D019098:Telomerase / Q000378:metabolism; D016615:Telomere / Q000235:genetics / Q000648:ultrastructure*
chemical_list: D019098:Telomerase
keywords: Chromosome arm; Q-FISH; TFU; Telomere; iPSC