title: Real time ligand-induced motion mappings of AChBP and nAChR using X-ray single molecule tracking.
abstract: We observed the dynamic three-dimensional (3D) single molecule behaviour of acetylcholine-binding protein (AChBP) and nicotinic acetylcholine receptor (nAChR) using a single molecule tracking technique, diffracted X-ray tracking (DXT) with atomic scale and 100 μs time resolution. We found that the combined tilting and twisting motions of the proteins were enhanced upon acetylcholine (ACh) binding. We present the internal motion maps of AChBP and nAChR in the presence of either ACh or α-bungarotoxin (αBtx), with views from two rotational axes. Our findings indicate that specific motion patterns represented as biaxial angular motion maps are associated with channel function in real time and on an atomic scale.
authors.affiliation: 1] CREST Sasaki Team, Japan Science and Technology Agency, The University of Tokyo, #609 Kiban Bldg., 5-1-5 Kashiwanoha, Kashiwa City, Chiba, 277-8561, Japan [2] Research &Utilization Division, Japan Synchrotron Radiation Research Institute, SPring-8 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sekiguchi
authors.affiliation: 1] CREST Sasaki Team, Japan Science and Technology Agency, The University of Tokyo, #609 Kiban Bldg., 5-1-5 Kashiwanoha, Kashiwa City, Chiba, 277-8561, Japan [2] Graduate School of Frontier Sciences, The University of Tokyo, Kiban Bldg., 5-1-5 Kashiwanoha, Kashiwa City, Chiba, 277-8561, Japan.
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: 1] Graduate School of Life Sciences, University of Hyogo, 3-2-1 Kouto, Kamigori-cho, Ako-gun, Hyogo, 679-1297, Japan [2] RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5148, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Nishino
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Suzuko
authors.initials: S
authors.lastname: Kobayashi
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Shimoyama
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Weiyan
authors.initials: W
authors.lastname: Cai
authors.affiliation: Graduate School of Frontier Sciences, The University of Tokyo, Kiban Bldg., 5-1-5 Kashiwanoha, Kashiwa City, Chiba, 277-8561, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Nagata
authors.affiliation: Graduate School of Frontier Sciences, The University of Tokyo, Kiban Bldg., 5-1-5 Kashiwanoha, Kashiwa City, Chiba, 277-8561, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Okada
authors.affiliation: 1] CREST Sasaki Team, Japan Science and Technology Agency, The University of Tokyo, #609 Kiban Bldg., 5-1-5 Kashiwanoha, Kashiwa City, Chiba, 277-8561, Japan [2] Graduate School of Frontier Sciences, The University of Tokyo, Kiban Bldg., 5-1-5 Kashiwanoha, Kashiwa City, Chiba, 277-8561, Japan.
authors.forename: Kouhei
authors.initials: K
authors.lastname: Ichiyanagi
authors.affiliation: Research &Utilization Division, Japan Synchrotron Radiation Research Institute, SPring-8 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198, Japan.
authors.forename: Noboru
authors.initials: N
authors.lastname: Ohta
authors.affiliation: 1] CREST Sasaki Team, Japan Science and Technology Agency, The University of Tokyo, #609 Kiban Bldg., 5-1-5 Kashiwanoha, Kashiwa City, Chiba, 277-8561, Japan [2] Research &Utilization Division, Japan Synchrotron Radiation Research Institute, SPring-8 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Yagi
authors.affiliation: 1] Graduate School of Life Sciences, University of Hyogo, 3-2-1 Kouto, Kamigori-cho, Ako-gun, Hyogo, 679-1297, Japan [2] RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5148, Japan.
authors.forename: Atsuo
authors.initials: A
authors.lastname: Miyazawa
authors.affiliation: 1] CREST Sasaki Team, Japan Science and Technology Agency, The University of Tokyo, #609 Kiban Bldg., 5-1-5 Kashiwanoha, Kashiwa City, Chiba, 277-8561, Japan [2] Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan [3] Molecular Profiling Research Center for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan.
authors.forename: Tai
authors.initials: T
authors.lastname: Kubo
authors.affiliation: 1] CREST Sasaki Team, Japan Science and Technology Agency, The University of Tokyo, #609 Kiban Bldg., 5-1-5 Kashiwanoha, Kashiwa City, Chiba, 277-8561, Japan [2] Research &Utilization Division, Japan Synchrotron Radiation Research Institute, SPring-8 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198, Japan [3] Graduate School of Frontier Sciences, The University of Tokyo, Kiban Bldg., 5-1-5 Kashiwanoha, Kashiwa City, Chiba, 277-8561, Japan.
authors.forename: Yuji C
authors.initials: YC
authors.lastname: Sasaki
mesh_terms: D000109:Acetylcholine / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D001048:Aplysia / Q000254:growth & development / Q000378:metabolism; D001665:Binding Sites; D002038:Bungarotoxins / Q000737:chemistry* / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D011487:Protein Conformation; D011978:Receptors, Nicotinic / Q000737:chemistry* / Q000378:metabolism; D014101:Torpedo / Q000254:growth & development / Q000378:metabolism
chemical_list: D002038:Bungarotoxins; D002352:Carrier Proteins; D011978:Receptors, Nicotinic; D000109:Acetylcholine