title: Elevated temperature differently affects foliar nitrogen partitioning in seedlings of diverse Douglas fir provenances.
abstract: Global climate change causes an increase in ambient air temperature, a major environmental factor influencing plant physiology and growth that already has been perceived at the regional scale and is expected to become even more severe in the future. In the present study, we investigated the effect of elevated ambient air temperature on the nitrogen metabolism of two interior provenances of Douglas ﬁr (Pseudotsuga menziesii var. glauca) originating from contrasting habitats, namely the provenances Monte Creek (MC) from a drier environment and Pend Oreille (PO) from a more humid environment. Three- to four-year-old seedlings of the two provenances were grown for 3 months in controlled environments under either control temperature (day 20 °C, night 15 °C) or high temperature (HT, 30/25 °C) conditions. Total nitrogen (N), soluble protein, chlorophyll and total amino acid (TAA) contents as well as individual amino acid concentrations were determined in both current-year and previous-year needles. Our results show that the foliar total N contents of the two provenances were unaffected by HT. Arginine, lysine, proline, glutamate and glutamine were the most abundant amino acids, which together contributed ∼88% to the TAA pool of current- and previous-year needles. High temperature decreased the contents of most amino acids of the glutamate family (i.e., arginine, proline, ornithine and glutamine) in current-year needles. However, HT did not affect the concentrations of metabolites related to the photorespiratory pathway, such as [Formula: see text], glycine and serine. In general, current-year needles were considerably more sensitive to HT than previous-year needles. Moreover, provenance PO originating from a mesic environment showed stronger responses to HT than provenance MC. Our results indicate provenance-specific plasticity in the response of Douglas fir to growth temperature. Provenance-specific effects of elevated temperature on N-use efficiency suggest that origin might determine the sensitivity and growth potential of Douglas fir trees in a future warmer climate.
authors.affiliation: Albert-Ludwigs-Universität Freiburg, Institut für Forstwissenschaften, Georges-Köhler-Allee Geb. 053/054, Freiburg i. Br. D-79110, Germany Sichuan Province Key Laboratory of Ecological Security and Protection, Mianyang Normal University, Xianren Road 30, Mianyang 621000, China.
authors.forename: Baoguo
authors.initials: B
authors.lastname: Du
authors.affiliation: Institute for Landscape Biogeochemistry, Leibniz Centre for Agricultural Landscape Research (ZALF), Eberswalderstr. 84, Müncheberg 15374, Germany.
authors.forename: Kirstin
authors.initials: K
authors.lastname: Jansen
authors.affiliation: Forstliche Versuchs- und Forschungsanstalt Baden-Württemberg, Wonnhaldestr. 4, Freiburg 79100, Germany Department of Biology, Graduate Program in Cell & Systems Biology, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga, ON, Canada.
authors.forename: Laura Verena
authors.initials: LV
authors.lastname: Junker
authors.affiliation: Albert-Ludwigs-Universität Freiburg, Institut für Forstwissenschaften, Georges-Köhler-Allee Geb. 053/054, Freiburg i. Br. D-79110, Germany.
authors.forename: Monika
authors.initials: M
authors.lastname: Eiblmeier
authors.affiliation: Albert-Ludwigs-Universität Freiburg, Institut für Forstwissenschaften, Georges-Köhler-Allee Geb. 053/054, Freiburg i. Br. D-79110, Germany juergen.kreuzwieser@ctp.uni-freiburg.de.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Kreuzwieser
authors.affiliation: Institute for Landscape Biogeochemistry, Leibniz Centre for Agricultural Landscape Research (ZALF), Eberswalderstr. 84, Müncheberg 15374, Germany Swiss Federal Research Institute WSL, Zürcherstr. 111, Birmensdorf 8903, Switzerland Berlin-Brandenburg Institute of Advanced Biodiversity Research (BBIB), Berlin 14195, Germany.
authors.forename: Arthur
authors.initials: A
authors.lastname: Gessler
authors.affiliation: Forstliche Versuchs- und Forschungsanstalt Baden-Württemberg, Wonnhaldestr. 4, Freiburg 79100, Germany Department of Biology, Graduate Program in Cell & Systems Biology, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga, ON, Canada.
authors.forename: Ingo
authors.initials: I
authors.lastname: Ensminger
authors.affiliation: Albert-Ludwigs-Universität Freiburg, Institut für Forstwissenschaften, Georges-Köhler-Allee Geb. 053/054, Freiburg i. Br. D-79110, Germany.
authors.forename: Heinz
authors.initials: H
authors.lastname: Rennenberg
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002734:Chlorophyll / Q000032:analysis; D006358:Hot Temperature; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism*; D028224:Pseudotsuga / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D002734:Chlorophyll; D009584:Nitrogen
keywords: Pseudotsuga menziesii; amino acid; arginine