title: The effect of high-fat diet and streptozotocin-induced diabetes and endurance training on plasma levels of calcitonin gene-related peptide and lactate in rats.
abstract: OBJECTIVE
  The aim of this study was to investigate the effect of type 2 diabetes induced by high-fat diet and streptozotocin, and the effect of endurance training on basal circulating levels of calcitonin gene-related peptide (CGRP) and lactate.
  
  
  METHODS
  Male Wistar rats were randomly divided into 4 groups: 1) control (n=8); 2) trained (n=8); 3) diabetic (n=8) and 4) trained diabetic (n=8). At the age of 7 weeks, diabetes was induced by feeding the animals a high-fat diet and injecting them with a low dose of streptozotocin (35 mg/kg). The animals at 10 weeks of age underwent an endurance training protocol on a treadmill for 7 weeks. Plasma lactate concentrations were measured by a lactate assay kit, and an enzyme immunoassay kit was used to measure CGRP.
  
  
  RESULTS
  The diabetic rats showed significant increases in plasma CGRP (3.0±1 ng/mL vs. 0.5±0.3 ng/mL, p<0.001) and plasma lactate levels (3.6±0.5 mmol/L vs. 1.3±0.5 mmol/L, p<0.001). Further, significant decrease in basal plasma lactate (2.6±0.5 mmol/L vs. 3.6±0.5 mmol/L, p<0.025) but not plasma CGRP levels (2.5±1.2 ng/mL vs. 3.0±1.3 ng/mL) were found in the diabetic subjects after the endurance training.
  
  
  CONCLUSIONS
  The results showed that endurance training could modify the basal circulating levels of lactate but not CGRP, which were elevated in this model of type 2 diabetic rats, indicating the lack of correspondence between the endurance training-induced changes of lactate and CGRP in this model of diabetes.
authors.affiliation: Department of Physical Education and Sports Sciences, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Daneshyar
authors.affiliation: Department of Physical Education and Sports Sciences, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran. Electronic address: ghara_re@modares.ac.ir.
authors.forename: Reza
authors.initials: R
authors.lastname: Gharakhanlou
authors.affiliation: Department of Exercise Physiology, Faculty of Physical Education and Sport Science, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Rohollah
authors.initials: R
authors.lastname: Nikooie
authors.affiliation: Department of Exercise Physiology, Faculty of Exercise Physiology and Sports Sciences, Razi University, Kermanshah, Iran.
authors.forename: Yazdan
authors.initials: Y
authors.lastname: Forutan
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000097:blood*; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000503:physiopathology*; D059305:Diet, High-Fat; D019344:Lactic Acid / Q000097:blood*; D008297:Male; D010805:Physical Conditioning, Animal / Q000502:physiology*; D010807:Physical Endurance / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D019344:Lactic Acid; D015740:Calcitonin Gene-Related Peptide
keywords: calcitonin gene-related peptide; diabetes; diabète; endurance training; entraînement d’endurance; lactate; peptide lié au gène de la calcitonine