title: High-quality combinatorial protein libraries using the binary patterning approach.
abstract: Protein combinatorial libraries have become a platform technology for exploring protein sequence space for novel molecules for use in research, synthetic biology, biotechnology, and medicine. To expedite the isolation of proteins with novel/desired functions using screens and selections, high-quality approaches that generate protein libraries rich in folded and soluble structures are desirable for this goal. The binary patterning approach is a protein library design method that incorporates elements of both rational design and combinatorial diversity to specify the arrangement of polar and nonpolar amino acid residues in the context of a desired, folded tertiary structure template. An overview of the considerations necessary to design and construct binary patterned libraries of de novo and natural proteins is presented.
authors.affiliation: Departments of Anatomy and Neurobiology, Molecular and Cellular Biochemistry, and the Center of Structural Biology, University of Kentucky College of Medicine, MN225 Chandler Medical Center, 800 Rose Street, Lexington, KY, 40536-0298, USA, lhbradley@uky.edu.
authors.forename: Luke H
authors.initials: LH
authors.lastname: Bradley
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D019151:Peptide Library; D015202:Protein Engineering / Q000379:methods; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D019151:Peptide Library; D011506:Proteins