title: Characterization and antioxidant activities of the polysaccharides from Radix Cyathulae officinalis Kuan.
abstract: A water-soluble polysaccharide (PSRC) was extracted and purified from the roots of Radix Cyathulae officinalis Kuan, and its chemical characteristics, monosaccharide composition and antioxidant activities were characterized. The average of molecular weight (Mw) of PSRC was 182 kDa. The majority of monosaccharide components of PSRC was glucose (relative mass 48.8%) with lower levels of mannose, rhamnose, galactose, fructose and arabinose (relative mass of 15.7, 14.3, 10.6, 6.1 and 4.5%, respectively). In vitro assays revealed that RSRC possessed potent scavenging activities against DPPH, hydroxyl and superoxide anion radicals. Oral administration of PSRC significantly enhanced antioxidant enzyme activities (including total superoxide dismutase, copper-zinc superoxide dismutase (Cu,Zn-SOD), manganese superoxide dismutase (Mn-SOD), glutathione peroxidase and catalase (CAT)) and capacities of scavenging superoxide anion and hydroxyl radicals, markedly lowered lipid peroxidation formation of malondialdehyde and significantly up-regulated mRNA expressions of Cu,Zn-SOD, Mn-SOD, CAT, glutathione peroxidase 1, thioredoxin 1 and thioredoxin 2, in a d-galactose-induced aging mouse model. Taken together, these findings demonstrate that PSRC could be used as a novel promising source of natural antioxidants and antiaging drugs.
authors.affiliation: Isotope Research Laboratory, Sichuan Agricultural University, Ya'an 625014, People's Republic of China.
authors.forename: Xingfa
authors.initials: X
authors.lastname: Han
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an 625014, People's Republic of China.
authors.forename: Shian
authors.initials: S
authors.lastname: Shen
authors.affiliation: Isotope Research Laboratory, Sichuan Agricultural University, Ya'an 625014, People's Republic of China.
authors.forename: Tieqiu
authors.initials: T
authors.lastname: Liu
authors.affiliation: Isotope Research Laboratory, Sichuan Agricultural University, Ya'an 625014, People's Republic of China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Du
authors.affiliation: Isotope Research Laboratory, Sichuan Agricultural University, Ya'an 625014, People's Republic of China.
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Cao
authors.affiliation: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, People's Republic of China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Feng
authors.affiliation: Isotope Research Laboratory, Sichuan Agricultural University, Ya'an 625014, People's Republic of China. Electronic address: xyzeng1966@163.com.
authors.forename: Xianyin
authors.initials: X
authors.lastname: Zeng
mesh_terms: D027703:Amaranthaceae / Q000737:chemistry; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D002374:Catalase / Q000096:biosynthesis; D005978:Glutathione / Q000096:biosynthesis; D005979:Glutathione Peroxidase / Q000096:biosynthesis; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D010084:Oxidation-Reduction / Q000187:drug effects*; D018517:Plant Roots / Q000737:chemistry; D011134:Polysaccharides / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D012333:RNA, Messenger / Q000096:biosynthesis; D013482:Superoxide Dismutase / Q000096:biosynthesis; D000094765:Glutathione Peroxidase GPX1; D000099156:Superoxide Dismutase 2
chemical_list: D000975:Antioxidants; D002374:Catalase; D005978:Glutathione; D005979:Glutathione Peroxidase; D011134:Polysaccharides; D012333:RNA, Messenger; D013482:Superoxide Dismutase; D000094765:Glutathione Peroxidase GPX1; D000099156:Superoxide Dismutase 2
keywords: Antioxidant activity; Cyathulae officinalis Kuan; Polysaccharides