title: Cisplatin inhibits the formation of a reactive intermediate during copper-catalyzed oxidation of amyloid β peptide.
abstract: Cisplatin was studied for its effect on the copper-catalyzed oxidation of amyloid β (Aβ) peptide. The interaction of cisplatin with Aβ1-16 in the presence of Cu(II) was investigated using cyclic voltammetry and mass spectrometry. The positive shift in the E1/2 value of Aβ1-16-Cu(II) suggests that the interaction of cisplatin alters the copper-binding properties of Aβ1-16. The mass spectrometry data show complete inhibition of copper-catalyzed decarboxylation/deamination of the Asp1 residue of Aβ1-16, while there is a significant decrease in copper-catalyzed oxidation of Aβ1-16 in the presence of cisplatin. Overall, our results provide a novel mode by which cisplatin inhibits copper-catalyzed oxidation of Aβ. These findings may lead to the design of better platinum complexes to treat oxidative stress in Alzheimer's disease and other related neurological disorders.
authors.affiliation: Biometry and Nutrition Group, Agharkar Research Institute , Pune, India.
authors.forename: Gulshan R
authors.initials: GR
authors.lastname: Walke
authors.forename: Srikanth
authors.initials: S
authors.lastname: Rapole
authors.forename: Prasad P
authors.initials: PP
authors.lastname: Kulkarni
mesh_terms: D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors*; D002384:Catalysis; D002945:Cisplatin / Q000737:chemistry / Q000494:pharmacology*; D003300:Copper / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D010084:Oxidation-Reduction
chemical_list: D016229:Amyloid beta-Peptides; D003300:Copper; D002945:Cisplatin