title: Growth kinetics, effect of carbon substrate in biosynthesis of mcl-PHA by Pseudomonas putida Bet001.
abstract: Growth associated biosynthesis of medium chain length poly-3-hydroxyalkanoates (mcl-PHA) in Pseudomonas putida Bet001 isolated from palm oil mill effluent was studied. Models with substrate inhibition terms described well the kinetics of its growth. Selected fatty acids (C8:0 to C18:1) and ammonium were used as carbon and nitrogen sources during growth and PHA biosynthesis, resulting in PHA accumulation of about 50 to 69% (w/w) and PHA yields ranging from 10.12 g L(-1) to 15.45 g L(-1), respectively. The monomer composition of the PHA ranges from C4 to C14, and was strongly influenced by the type of carbon substrate fed. Interestingly, an odd carbon chain length (C7) monomer was also detected when C18:1 was fed. Polymer showed melting temperature (T m) of 42.0 (± 0.2) °C, glass transition temperature (T g) of -1.0 (± 0.2) °C and endothermic melting enthalpy of fusion (ΔHf) of 110.3 (± 0.1) J g(-1). The molecular weight (M w) range of the polymer was relatively narrow between 55 to 77 kDa.
authors.affiliation: Institute of Biological Sciences Faculty of Science University of Malaya Kuala Lumpur Malaysia Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: A M
authors.initials: AM
authors.lastname: Gumel
authors.affiliation: Institute of Biological Sciences Faculty of Science University of Malaya Kuala Lumpur Malaysia Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: M S M
authors.initials: MS
authors.lastname: Annuar
authors.affiliation: Department of Chemistry Faculty of Science University of Malaya Kuala Lumpur Malaysia Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: T
authors.initials: T
authors.lastname: Heidelberg
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004783:Environmental Microbiology; D005227:Fatty Acids / Q000378:metabolism; D007218:Industrial Microbiology; D007220:Industrial Waste; D054813:Polyhydroxyalkanoates / Q000378:metabolism*; D016958:Pseudomonas putida / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D013696:Temperature
chemical_list: D064751:Ammonium Compounds; D003470:Culture Media; D005227:Fatty Acids; D007220:Industrial Waste; D054813:Polyhydroxyalkanoates; D002244:Carbon
keywords: Pseudomonas; biopolymers; kinetics; polyesters; polyhydroxyalkanoates