title: Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats.
abstract: OBJECTIVE
  To investigate the experimental model for obtaining adipose tissue, isolation, characterization of mesenchymal stem cells and evaluation of their distribution in the tram flap in rats.
  
  
  METHODS
  Five rats of Wistar were randomly assigned to two groups. In group I, three animals underwent removal of adipose tissue in the groin procedure to establish the experimental model and obtain a cell lineage. The animals of group II (n = 2) underwent surgical flap procedure, and satisfaction injection of mesenchymal stem cells pretreated with marker fluorescente.
  
  
  RESULTS
  obtaining adipose tissue of the inguinal region of the rat proved to be possible. The isolated cells were characterized as mesenchymal stem cells and fluorescence microscopy showed the presence of multiple cells arranged around blood vessels and capillaries.
  
  
  CONCLUSIONS
  It was possible to establish an experimental model for obtaining adipose tissue for isolation of mesenchymal stem cells and their distribution in the TRAM flap in rats.
authors.affiliation: Federal University of Sao Paulo, Brazil.
authors.forename: André Luiz Pires de
authors.initials: AL
authors.lastname: Freitas
authors.affiliation: Federal University of Sao Paulo, Brazil.
authors.forename: Marcelo de
authors.initials: Md
authors.lastname: Oliveira e Silva
authors.affiliation: UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Priscilla Martin Keiko
authors.initials: PM
authors.lastname: Matsumoto
authors.affiliation: UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Sang Won
authors.initials: SW
authors.lastname: Han
authors.affiliation: UNIFESP, Sao Paulo, SP, Brasil.
authors.forename: Lydia Masako
authors.initials: LM
authors.lastname: Ferreira
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D016130:Immunophenotyping; D008297:Male; D059630:Mesenchymal Stem Cells; D008856:Microscopy, Fluorescence; D023421:Models, Animal; D011897:Random Allocation; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013524:Surgical Flaps