title: CD44 but not CD24 expression is related to poor prognosis in non-cardia adenocarcinoma of the stomach.
abstract: BACKGROUND
  Recent studies have focused on the diagnostic and prognostic significance of CD24 and CD44 expression in human cancers. This study aimed to explore changes in CD44 and CD24 expression levels in patients with gastric cancer and to assess their prognostic values.
  
  
  METHODS
  CD44 and CD24 expression levels were investigated immunohistochemically in tumor samples from 290 patients with non-cardia gastric adenocarcinoma, of whom 77 had paired adjacent normal gastric mucosa. CD24 and CD44 mRNA levels were determined by quantitative polymerase chain reaction in 34 patients. Serum anti-Helicobacter pylori IgG was detected by enzyme-linked immunosorbent assay. Relationships between CD44 and CD24 protein expression levels and tumor parameters were analyzed and their prognostic values were evaluated by Cox proportional hazards models.
  
  
  RESULTS
  CD24 and CD44 expression levels were significantly higher in patients with gastric cancer compared with those in paired controls (45.5% vs. 0.0%, and 61.0% vs. 0.0%, P < 0.001). Among 290 patients, the overall survival rate was significantly higher in CD44(-) compared with CD44(+) patients (log-rank test, P = 0.035). However, there was no significant correlation between CD24 expression and overall survival time (log-rank test, P = 0.115). Multivariate regression analysis indicated that positive CD44 expression (P = 0.029), TNM staging (P < 0.001), and lymphovascular invasion (P = 0.016), but not CD24 expression (P = 0.065), were independent prognostic factors in gastric cancer.
  
  
  CONCLUSIONS
  Individual expression of CD44 was associated with poor survival in patients with gastric carcinoma.
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Cao
authors.forename: Donghui
authors.initials: D
authors.lastname: Cao
authors.forename: MeiShan
authors.initials: M
authors.lastname: Jin
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Jia
authors.forename: Fei
authors.initials: F
authors.lastname: Kong
authors.forename: Hongxi
authors.initials: H
authors.lastname: Ma
authors.forename: Yinping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Division of Clinical Epidemiology, First Hospital of Jilin University, Changchun, Jilin 130021, China. jiangjing19702000@jlu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Jiang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D051927:CD24 Antigen / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D011379:Prognosis; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology
chemical_list: D051927:CD24 Antigen; C497889:CD24 protein, human; C497483:CD44 protein, human; D018960:Hyaluronan Receptors; D012333:RNA, Messenger