title: A novel EGFP-expressing nude mice with complete loss of lymphocytes and NK cells to study tumor-host interactions.
abstract: Enhanced green fluorescent protein (EGFP) expressing Balb/c nude mice strain with Rag-2 and Jak3 double mutants (Nude-R/J-EGFP mice) was established to improve the take rate of human tumors and to distinguish tumor and host cells. EGFP was ubiquitously expressed in all organs including the brain, lung, liver, heart, kidney, spleen, and gastrointestinal tract in Nude-R/J-EGFP mice. The mice showed complete loss of T lymphocytes, B lymphocytes, and NK cells, indicating a higher take rate of human tumor xenograft. M213-mCherry, an mCherry expressing the cholangiocarcinoma cell line, was successfully detected and tumor vessels derived from the host were clearly identified with fluorescence imager. Thus, dual-color fluorescence imaging visualizes the tumor-host interaction by non-invasive in vivo fluorescent imaging in Nude-R/J-EGFP mice. These finding suggests that Nude-R/J-EGFP mice are becoming a powerful tool to investigate human tumor-host interactions.
authors.affiliation: Division of Hematopoiesis, Center for AIDS Research, Kumamoto University.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Gotoh
authors.forename: Ryusho
authors.initials: R
authors.lastname: Kariya
authors.forename: Kouki
authors.initials: K
authors.lastname: Matsuda
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Hattori
authors.forename: Kulthida
authors.initials: K
authors.lastname: Vaeteewoottacharn
authors.forename: Seiji
authors.initials: S
authors.lastname: Okada
mesh_terms: D000818:Animals; D001650:Bile Duct Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D001653:Bile Ducts, Intrahepatic / Q000276:immunology / Q000473:pathology*; D045744:Cell Line, Tumor; D018281:Cholangiocarcinoma / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D004268:DNA-Binding Proteins / Q000235:genetics; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D053616:Janus Kinase 3 / Q000235:genetics; D007694:Killer Cells, Natural / Q000276:immunology; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D009154:Mutation; D061848:Optical Imaging; D013601:T-Lymphocytes / Q000276:immunology*; D023041:Xenograft Model Antitumor Assays
chemical_list: D004268:DNA-Binding Proteins; C499308:Rag2 protein, mouse; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C507935:Jak3 protein, mouse; D053616:Janus Kinase 3