title: Green tea aroma fraction reduces β-amyloid peptide-induced toxicity in Caenorhabditis elegans transfected with human β-amyloid minigene.
abstract: Green tea is a popular world-wide beverage with health benefits that include preventive effects on cancer as well as cardiovascular, liver and Alzheimer's diseases (AD). This study will examine the preventive effects on AD of a unique aroma of Japanese green tea. First, a transgenic Caenorhabditis elegans (C. elegans) CL4176 expressing human β-amyloid peptide (Aβ) was used as a model of AD. A hexane extract of processed green tea was further fractionated into volatile and non-volatile fractions, named roasty aroma and green tea aroma fractions depending on their aroma, by microscale distillation. Both hexane extract and green tea aroma fraction were found to inhibit Aβ-induced paralysis, while only green tea aroma fraction extended lifespan in CL4176. We also found that green tea aroma fraction has antioxidant activity. This paper indicates that the green tea aroma fraction is an additional component for prevention of AD.
authors.affiliation: a Research Institute for Clinical Oncology , Saitama Cancer Center , Saitama , Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Takahashi
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Watanabe
authors.forename: Takashi
authors.initials: T
authors.lastname: Fujita
authors.forename: Toshio
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Michio
authors.initials: M
authors.lastname: Saito
authors.forename: Masami
authors.initials: M
authors.lastname: Suganuma
mesh_terms: D000544:Alzheimer Disease / Q000517:prevention & control; D016229:Amyloid beta-Peptides / Q000235:genetics* / Q000633:toxicity*; D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects*; D006586:Hexanes / Q000737:chemistry; D006801:Humans; D008136:Longevity / Q000187:drug effects; D009812:Odorants; D010243:Paralysis / Q000139:chemically induced / Q000517:prevention & control; D010936:Plant Extracts / Q000494:pharmacology*; D013662:Tea / Q000737:chemistry*; D014162:Transfection
chemical_list: D016229:Amyloid beta-Peptides; D006586:Hexanes; D010936:Plant Extracts; D013662:Tea
keywords: Alzheimer’s disease; lifespan; nematodes; paralysis; β-amyloid peptide