title: Principles and application of antibody libraries for infectious diseases.
abstract: Antibodies have been used efficiently for the treatment and diagnosis of many diseases. Recombinant antibody technology allows the generation of fully human antibodies. Phage display is the gold standard for the production of human antibodies in vitro. To generate monoclonal antibodies by phage display, the generation of antibody libraries is crucial. Antibody libraries are classified according to the source where the antibody gene sequences were obtained. The most useful library for infectious diseases is the immunized library. Immunized libraries would allow better and selective enrichment of antibodies against disease antigens. The antibodies generated from these libraries can be translated for both diagnostic and therapeutic applications. This review focuses on the generation of immunized antibody libraries and the potential applications of the antibodies derived from these libraries.
authors.affiliation: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia, lbeenar@gmail.com.
authors.forename: Bee Nar
authors.initials: BN
authors.lastname: Lim
authors.forename: Gee Jun
authors.initials: GJ
authors.lastname: Tye
authors.forename: Yee Siew
authors.initials: YS
authors.lastname: Choong
authors.forename: Eugene Boon Beng
authors.initials: EB
authors.lastname: Ong
authors.forename: Asma
authors.initials: A
authors.lastname: Ismail
authors.forename: Theam Soon
authors.initials: TS
authors.lastname: Lim
mesh_terms: D000911:Antibodies, Monoclonal / Q000235:genetics / Q000627:therapeutic use*; D003141:Communicable Diseases / Q000628:therapy*; D006801:Humans; D019151:Peptide Library; D011994:Recombinant Proteins / Q000235:genetics / Q000627:therapeutic use; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D019151:Peptide Library; D011994:Recombinant Proteins