title: Biomarkers of oxidative stress to nucleic acids: background levels and effects of body mass index and life-style factors in an urban paediatric population.
abstract: The aims of the present study were to establish the background levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), 8-oxo-7,8-dihydroguanosine (8-oxoGuo), 8-oxo-7,8-dihydroguanine (8-oxoGua) among a group of healthy Italian children, and to evaluate the contribution of some potential interfering/confounding factors to the urinary (u) levels of these biomarkers of oxidised guanine derivatives. The levels of 8-oxodGuo, 8-oxoGuo, 8-oxoGua, and u-cotinine in urine samples from 159 healthy children (5-11years) recruited in a cross-sectional study were measured via liquid chromatography-tandem mass spectrometry. Data regarding the anthropometric and life-style characteristics of the participants were obtained from questionnaires. The 5th-95th percentiles of the levels of 8-oxodGuo, 8-oxoGuo, and 8-oxoGua for all children were 2.4-13.9, 3.8-19.9 and 5.4-79.5μg/L and 2.9-12.6, 4.8-15.2, and 5.1-93.4μg/g creatinine, respectively. Significant correlations were found between the level of 8-oxoGuo and that of 8-oxoGua and 8-oxodGuo but not between the level of 8-oxoGua and that of 8-oxodGuo in all children and in both the male and female subgroups. Multiple linear regression analyses revealed the independent effect of the investigated variables on 8-oxodGuo, 8-oxoGuo, and 8-oxoGua. u-Creatinine was the most significant predictor of the urinary excretion of both 8-oxoGuo and 8-oxodGuo, age displayed a significant positive independent effect on the level of 8-oxoGuo, whereas the weight status according to the BMI was negatively associated with the level of 8-oxodGuo. None of the chosen independent variables influenced the levels of 8-oxoGua.
authors.affiliation: Department of Public Health and Infectious Diseases, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. Electronic address: carmela.protano@uniroma1.it.
authors.forename: Carmela
authors.initials: C
authors.lastname: Protano
authors.affiliation: Laboratory of Industrial Toxicology, Department of Clinical and Experimental Medicine, University of Parma, via Gramsci 14, I-43126 Parma, Italy. Electronic address: roberta.andreoli@unipr.it.
authors.forename: Roberta
authors.initials: R
authors.lastname: Andreoli
authors.affiliation: Laboratory of Industrial Toxicology, Department of Clinical and Experimental Medicine, University of Parma, via Gramsci 14, I-43126 Parma, Italy. Electronic address: antonio.mutti@unipr.it.
authors.forename: Antonio
authors.initials: A
authors.lastname: Mutti
authors.affiliation: Department of Public Health and Infectious Diseases, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. Electronic address: stefano.petti@uniroma1.it.
authors.forename: Stefano
authors.initials: S
authors.lastname: Petti
authors.affiliation: Department of Public Health and Infectious Diseases, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. Electronic address: matteo.vitali@uniroma1.it.
authors.forename: Matteo
authors.initials: M
authors.lastname: Vitali
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D015415:Biomarkers / Q000652:urine; D015992:Body Mass Index; D002648:Child; D003367:Cotinine / Q000652:urine; D003430:Cross-Sectional Studies; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D004781:Environmental Exposure / Q000706:statistics & numerical data*; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives / Q000652:urine; D006151:Guanosine / Q000031:analogs & derivatives / Q000652:urine; D006801:Humans; D008019:Life Style; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D014505:Urban Population / Q000706:statistics & numerical data*
chemical_list: D015415:Biomarkers; D006151:Guanosine; C046215:8-hydroxyguanosine; C024829:8-hydroxyguanine; D006147:Guanine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D003367:Cotinine
keywords: Biomarkers; Body mass index; Children; Oxidative stress; Urinary oxidised guanine derivatives