title: Characteristics of patients recently infected with HIV-1 non-B subtypes in France: a nested study within the mandatory notification system for new HIV diagnoses.
abstract: The presence of HIV-1 non-B subtypes in Western Europe is commonly attributed to migration of individuals from non-European countries, but the possible role of domestic infections with non-B subtypes is not well investigated. The French mandatory anonymous reporting system for HIV is linked to a virological surveillance using assays for recent infection (<6 months) and serotyping. During the first semester of years 2007 to 2010, any sample corresponding to a non-B recent infection was analyzed by sequencing a 415-bp env region, followed by phylogenetic analysis and search for transmission clusters. Two hundred thirty-three recent HIV-1 infections with non-B variants were identified. They involved 5 subtypes and 7 circulating recombinant forms (CRFs). Ninety-two cases (39.5%) were due to heterosexual transmissions, of which 39 occurred in patients born in France. Eighty-five cases (36.5%) were identified in men having sex with men (MSM). Forty-three recent non-B infections (18.5%) segregated into 14 clusters, MSM being involved in 11 of them. Clustered transmission events included 2 to 7 cases per cluster. The largest cluster involved MSM infected by a CRF02_AG variant. In conclusion, we found that the spread of non-B subtypes in France occurs in individuals of French origin and that MSM are particularly involved in this dynamic.
authors.affiliation: Centre National de Référence du VIH, laboratoire de Virologie, CHRU de Tours, France Inserm U966, Université François Rabelais, Tours, France.
authors.forename: Denys
authors.initials: D
authors.lastname: Brand
authors.affiliation: Inserm U966, Université François Rabelais, Tours, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Moreau
authors.affiliation: Institut de Veille Sanitaire, Saint-Maurice, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Cazein
authors.affiliation: Institut de Veille Sanitaire, Saint-Maurice, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Lot
authors.affiliation: Institut de Veille Sanitaire, Saint-Maurice, France.
authors.forename: Josiane
authors.initials: J
authors.lastname: Pillonel
authors.affiliation: Centre National de Référence du VIH, laboratoire de Virologie, CHRU de Tours, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Brunet
authors.affiliation: Centre National de Référence du VIH, laboratoire de Virologie, CHRU de Tours, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Thierry
authors.affiliation: Institut de Veille Sanitaire, Saint-Maurice, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Le Vu
authors.affiliation: Laboratoire associé au Centre National de Référence du VIH, CHU de Rouen and EA 2656, Université de Rouen, Rouen, France.
authors.forename: Jean-Christophe
authors.identifier: http://orcid.org/0000-0001-6417-4083
authors.initials: JC
authors.lastname: Plantier
authors.affiliation: Institut de Veille Sanitaire, Saint-Maurice, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Semaille
authors.affiliation: Centre National de Référence du VIH, laboratoire de Virologie, CHRU de Tours, France Inserm U966, Université François Rabelais, Tours, France fbarin@med.univ-tours.fr.
authors.forename: Francis
authors.initials: F
authors.lastname: Barin
mesh_terms: D016000:Cluster Analysis; D005260:Female; D005602:France / Q000453:epidemiology; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology / Q000635:transmission* / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics
chemical_list: D054299:env Gene Products, Human Immunodeficiency Virus