title: Excretion of urinary orosomucoid 1 protein is elevated in patients with chronic heart failure.
abstract: Easily screening markers for early detection of chronic heart failure (CHF) are lacking. We identified twenty differently expressed proteins including orosomucoid 1(ORM1) in urine between patients with CHF and normal controls by proteomic methods. Bioinformatics analyses suggested ORM1 could be used for further analysis. After verification by western blotting, the urinary levels of ORM1 were quantified with enzyme-linked immunosorbent assay (ELISA) by correcting for creatinine expression. The ORM1-Cr was significantly elevated in CHF patients than normal controls (6498.83 ± 4300.21 versus 2102.26 ± 1069.24 ng/mg). Furthermore, a Spearman analysis indicated that the urinary ORM1 levels had a high positive correlation with the classification of CHF, and the multivariate analysis suggested that the urinary ORM1 content was associated with the plasma amino-terminal pro- brain natriuretic peptide (NT-proBNP) (OR: 2.106, 95% CI: 1.213-3.524, P = 0.002) and the New York Heart Association (NYHA) classification (OR: 3.019, 95% CI: 1.329-4.721, P<0.001). In addition, receiving operating curve (ROC) analyses suggested that an optimum cut-off value of 2484.98 ng/mg with 90.91% sensitivity and 85.48% specificity, respectively, could be used for the diagnosis of CHF. To sum up, our findings indicate that ORM1 could be a potential novel urinary biomarker for the early detection of CHF.
authors.affiliation: State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R.China; Department of healthy management, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R.China.
authors.forename: Li-na
authors.initials: LN
authors.lastname: Hou
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R.China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.affiliation: State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R.China.
authors.forename: Qing-chun
authors.initials: QC
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R.China.
authors.forename: Liang
authors.initials: L
authors.lastname: Su
authors.affiliation: State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R.China.
authors.forename: Ping-an
authors.initials: PA
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R.China.
authors.forename: Zhi-hao
authors.initials: ZH
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R.China.
authors.forename: Din-ji
authors.initials: DJ
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R.China.
authors.forename: Chang-hua
authors.initials: CH
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R.China.
authors.forename: Ding-li
authors.initials: DL
authors.lastname: Xu
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000652:urine*; D002908:Chronic Disease; D003404:Creatinine / Q000652:urine; D005260:Female; D006333:Heart Failure / Q000652:urine*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009961:Orosomucoid / Q000652:urine*
chemical_list: D015415:Biomarkers; D009961:Orosomucoid; D003404:Creatinine