title: Simultaneous purification of limonin, nomilin and isoobacunoic acid from pomelo fruit (Citrus grandis) segment membrane.
abstract: A method was established for purification of limonin, nomilin, and isoobacunoic acid simultaneously from segment membranes of pomelo (Citrus Grandis). This method includes 3 steps, removing most impurities by macroporous resin HZ-816, isolating limonin by High Speed Counter Current Chromatography (HSCCC), and isolating nomilin and isoobacunoic acid by semi-preparative HPLC. Naringin was partially purified as a by-product of this process using Sephadex LH-20. All limonoids purified through this method reached 95% purity. The purified limonin, nomilin and isoobacunoic acid were identified according to the retention time of the standard substances using HPLC and characteristic fragment ions of LC-MS/MS.
  
  
  CONCLUSIONS
  A method of getting health care products limonoids from fruit processing by-products segments membrane of pomelo.
authors.affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang Univ, Zijingang Campus, Hangzhou, 310058, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiang
authors.forename: Jinping
authors.initials: J
authors.lastname: Cao
authors.forename: Fenglei
authors.initials: F
authors.lastname: Luo
authors.forename: Dengliang
authors.initials: D
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.forename: Chongde
authors.initials: C
authors.lastname: Sun
authors.forename: Kunsong
authors.initials: K
authors.lastname: Chen
mesh_terms: D001584:Benzoxepins / Q000737:chemistry*; D002853:Chromatography, Liquid; D002957:Citrus / Q000737:chemistry*; D044950:Flavanones; D005638:Fruit / Q000737:chemistry*; D036701:Limonins / Q000737:chemistry*; D013058:Mass Spectrometry; D053719:Tandem Mass Spectrometry
chemical_list: D001584:Benzoxepins; D044950:Flavanones; D036701:Limonins; C000598032:isoobacunoic acid; C059405:nomilin; C001546:limonin; C005274:naringin
keywords: limonoids; pomelo; purification