title: Composition and antiproliferative effect of essential oil of Origanum vulgare against tumor cell lines.
abstract: Cancer is a leading cause of death and is responsible for one in eight deaths worldwide. The use of herbs as complementary medicine for cancer, especially advanced cancer, has recently increased. The aim of this study was to evaluate in vitro, the antiproliferative effect of Origanum vulgare against human breast adenocarcinoma (MCF-7), and human colon adenocarcinoma (HT-29). The essential oil (EO) was extracted from a bought amount of O. vulgare dried leaves and analyzed in a gas chromatograph interfaced with a mass selective detector. The cytotoxicity test was performed by sulforhodamine B assay. The results show that the EO is composed mostly of 4-terpineol and induces a high cytotoxicity effect in HT-29. In the MCF-7 cell line the EO was less effective. In conclusion, this study showed that O. vulgare main component is 4-terpineol and was effective in inducing cancer cell growth inhibition.
authors.affiliation: 1 Department of Physiology and Pharmacology, Institute of Biology, Federal University of Pelotas , Pelotas, Rio Grande do Sul, Brazil .
authors.forename: Karine Rech
authors.initials: KR
authors.lastname: Begnini
authors.forename: Fernanda
authors.initials: F
authors.lastname: Nedel
authors.forename: Rafael Guerra
authors.initials: RG
authors.lastname: Lund
authors.forename: Pedro Henrique de Azambuja
authors.initials: PH
authors.lastname: Carvalho
authors.forename: Maria Regina Alves
authors.initials: MR
authors.lastname: Rodrigues
authors.forename: Fátima Tereza Alves
authors.initials: FT
authors.lastname: Beira
authors.forename: Francisco Augusto Burkert
authors.initials: FA
authors.lastname: Del-Pino
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D006131:Growth Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D061986:MCF-7 Cells; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*; D031341:Origanum / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006131:Growth Inhibitors; D009822:Oils, Volatile; D010936:Plant Extracts
keywords: Origanum vulgare; antiproliferative assay; cancer cell lines; plant extracts