title: A case of culture-negative endocarditis due to Streptococcus tigurinus.
abstract: Culture-negative endocarditis remains a diagnostic and therapeutic challenge despite recent medical advances. Streptococcus tigurinus, a novel member of the Streptococcus mitis group, was first identified in Zurich. S. tigurinus possesses virulence determinants and causes invasive infections. We report a case of culture-negative endocarditis with serious complications due to S. tigurinus, which was identified by 16S ribosomal RNA gene sequence analysis of excised valve tissue specimens. This technique is useful for identification of the causative microorganism in patients with culture-negative endocarditis and may facilitate early diagnosis and appropriate antimicrobial treatment.
authors.affiliation: Department of Infection Control and Laboratory Diagnostics, Internal Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan; Division of Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, USA. Electronic address: kanamori@med.tohoku.ac.jp.
authors.forename: Hajime
authors.initials: H
authors.lastname: Kanamori
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Risako
authors.initials: R
authors.lastname: Kakuta
authors.affiliation: Department of Infection Control and Laboratory Diagnostics, Internal Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Yano
authors.affiliation: Department of Cardiovascular Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Department of Infection Control and Laboratory Diagnostics, Internal Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Department of Infection Control and Laboratory Diagnostics, Internal Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Oe
authors.affiliation: Department of Infection Control and Laboratory Diagnostics, Internal Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Inomata
authors.affiliation: Department of Infection Control and Laboratory Diagnostics, Internal Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Aoyagi
authors.affiliation: Department of Infection Control and Laboratory Diagnostics, Internal Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Masumitsu
authors.initials: M
authors.lastname: Hatta
authors.affiliation: Department of Infection Control and Laboratory Diagnostics, Internal Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Shiro
authors.initials: S
authors.lastname: Endo
authors.affiliation: Department of Infection Control and Laboratory Diagnostics, Internal Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Tokuda
authors.affiliation: Division of Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Weber
authors.affiliation: Department of Pathology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: Department of Cardiovascular Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Saiki
authors.affiliation: Department of Infection Control and Laboratory Diagnostics, Internal Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Kaku
mesh_terms: D000328:Adult; D004269:DNA, Bacterial / Q000235:genetics; D004697:Endocarditis, Bacterial / Q000382:microbiology*; D006351:Heart Valves / Q000382:microbiology / Q000601:surgery; D006801:Humans; D008297:Male; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013290:Streptococcal Infections / Q000382:microbiology*; D013291:Streptococcus / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: 16S ribosomal RNA gene analysis; Culture-negative endocarditis; Endocarditis; Streptococcus; Streptococcus tigurinus