title: Dysregulated mature IL-1β production in familial Mediterranean fever.
abstract: OBJECTIVE
  The aim of this study was to analyse the role of circulating cleaved IL-1β in patients with FMF.
  
  
  METHODS
  We enrolled 20 patients with FMF (5 males and 15 females), 22 patients with RA (4 males and 18 females) and 22 healthy controls (6 males and 16 females). Serum levels of serum amyloid A (SAA) were measured by ELISA. We also determined whether IL-1β was present as the cleaved form (p17) in the sera of FMF patients by immunoblotting using anti-cleaved IL-1β antibody.
  
  
  RESULTS
  Although SAA concentrations were elevated in the sera, there was no significant difference in these concentrations between FMF patients and RA patients. Immunoblot analysis demonstrated that the cleaved form of IL-1β (p17) was present in sera from FMF patients during febrile attack periods, but not in healthy controls. Bands representing the cleaved form of IL-1β were not detected in serum from FMF patients at non-febrile attack periods or remission periods under colchicine treatment. The amounts of cleaved IL-1β (p17) were significantly higher in patients with FMF compared with those in patients with RA in the inflammatory phase.
  
  
  CONCLUSIONS
  The cleaved form of IL-1β is a valuable biomarker for monitoring disease activity and response to colchicine treatment in patients with FMF. It might be useful to discriminate FMF from other non-IL-1β-mediated inflammatory disorders.
authors.affiliation: Department of Rheumatology and Clinical Research Center, Nagasaki Medical Center, Omura, Department of Rheumatology, Isahaya Health Insurance General Hospital, Nagasaki, Department of Infection and Host Defense, Graduate School of Medicine, Shinshu University, Matsumoto, Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Ehime, Department of Rheumatology, Sasebo City General Hospital, Sasebo, Department of Rheumatology, Shinto Kumamoto Hospital, Kumamoto, Clinical Research Center, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Institute of Tropical Medicine (NEKKEN), Nagasaki University and Department of Rheumatology, Nagasaki University Hospital, Nagasaki, Japan. migita@nagasaki-mc.com.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Migita
authors.affiliation: Department of Rheumatology and Clinical Research Center, Nagasaki Medical Center, Omura, Department of Rheumatology, Isahaya Health Insurance General Hospital, Nagasaki, Department of Infection and Host Defense, Graduate School of Medicine, Shinshu University, Matsumoto, Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Ehime, Department of Rheumatology, Sasebo City General Hospital, Sasebo, Department of Rheumatology, Shinto Kumamoto Hospital, Kumamoto, Clinical Research Center, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Institute of Tropical Medicine (NEKKEN), Nagasaki University and Department of Rheumatology, Nagasaki University Hospital, Nagasaki, Japan.
authors.forename: Yasumori
authors.initials: Y
authors.lastname: Izumi
authors.affiliation: Department of Rheumatology and Clinical Research Center, Nagasaki Medical Center, Omura, Department of Rheumatology, Isahaya Health Insurance General Hospital, Nagasaki, Department of Infection and Host Defense, Graduate School of Medicine, Shinshu University, Matsumoto, Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Ehime, Department of Rheumatology, Sasebo City General Hospital, Sasebo, Department of Rheumatology, Shinto Kumamoto Hospital, Kumamoto, Clinical Research Center, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Institute of Tropical Medicine (NEKKEN), Nagasaki University and Department of Rheumatology, Nagasaki University Hospital, Nagasaki, Japan.
authors.forename: Keita
authors.initials: K
authors.lastname: Fujikawa
authors.affiliation: Department of Rheumatology and Clinical Research Center, Nagasaki Medical Center, Omura, Department of Rheumatology, Isahaya Health Insurance General Hospital, Nagasaki, Department of Infection and Host Defense, Graduate School of Medicine, Shinshu University, Matsumoto, Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Ehime, Department of Rheumatology, Sasebo City General Hospital, Sasebo, Department of Rheumatology, Shinto Kumamoto Hospital, Kumamoto, Clinical Research Center, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Institute of Tropical Medicine (NEKKEN), Nagasaki University and Department of Rheumatology, Nagasaki University Hospital, Nagasaki, Japan.
authors.forename: Kazunaga
authors.initials: K
authors.lastname: Agematsu
authors.affiliation: Department of Rheumatology and Clinical Research Center, Nagasaki Medical Center, Omura, Department of Rheumatology, Isahaya Health Insurance General Hospital, Nagasaki, Department of Infection and Host Defense, Graduate School of Medicine, Shinshu University, Matsumoto, Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Ehime, Department of Rheumatology, Sasebo City General Hospital, Sasebo, Department of Rheumatology, Shinto Kumamoto Hospital, Kumamoto, Clinical Research Center, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Institute of Tropical Medicine (NEKKEN), Nagasaki University and Department of Rheumatology, Nagasaki University Hospital, Nagasaki, Japan.
authors.forename: Junya
authors.initials: J
authors.lastname: Masumoto
authors.affiliation: Department of Rheumatology and Clinical Research Center, Nagasaki Medical Center, Omura, Department of Rheumatology, Isahaya Health Insurance General Hospital, Nagasaki, Department of Infection and Host Defense, Graduate School of Medicine, Shinshu University, Matsumoto, Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Ehime, Department of Rheumatology, Sasebo City General Hospital, Sasebo, Department of Rheumatology, Shinto Kumamoto Hospital, Kumamoto, Clinical Research Center, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Institute of Tropical Medicine (NEKKEN), Nagasaki University and Department of Rheumatology, Nagasaki University Hospital, Nagasaki, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Jiuchi
authors.affiliation: Department of Rheumatology and Clinical Research Center, Nagasaki Medical Center, Omura, Department of Rheumatology, Isahaya Health Insurance General Hospital, Nagasaki, Department of Infection and Host Defense, Graduate School of Medicine, Shinshu University, Matsumoto, Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Ehime, Department of Rheumatology, Sasebo City General Hospital, Sasebo, Department of Rheumatology, Shinto Kumamoto Hospital, Kumamoto, Clinical Research Center, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Institute of Tropical Medicine (NEKKEN), Nagasaki University and Department of Rheumatology, Nagasaki University Hospital, Nagasaki, Japan.
authors.forename: Hideko
authors.initials: H
authors.lastname: Kozuru
authors.affiliation: Department of Rheumatology and Clinical Research Center, Nagasaki Medical Center, Omura, Department of Rheumatology, Isahaya Health Insurance General Hospital, Nagasaki, Department of Infection and Host Defense, Graduate School of Medicine, Shinshu University, Matsumoto, Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Ehime, Department of Rheumatology, Sasebo City General Hospital, Sasebo, Department of Rheumatology, Shinto Kumamoto Hospital, Kumamoto, Clinical Research Center, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Institute of Tropical Medicine (NEKKEN), Nagasaki University and Department of Rheumatology, Nagasaki University Hospital, Nagasaki, Japan.
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Nonaka
authors.affiliation: Department of Rheumatology and Clinical Research Center, Nagasaki Medical Center, Omura, Department of Rheumatology, Isahaya Health Insurance General Hospital, Nagasaki, Department of Infection and Host Defense, Graduate School of Medicine, Shinshu University, Matsumoto, Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Ehime, Department of Rheumatology, Sasebo City General Hospital, Sasebo, Department of Rheumatology, Shinto Kumamoto Hospital, Kumamoto, Clinical Research Center, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Institute of Tropical Medicine (NEKKEN), Nagasaki University and Department of Rheumatology, Nagasaki University Hospital, Nagasaki, Japan.
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Shimizu
authors.affiliation: Department of Rheumatology and Clinical Research Center, Nagasaki Medical Center, Omura, Department of Rheumatology, Isahaya Health Insurance General Hospital, Nagasaki, Department of Infection and Host Defense, Graduate School of Medicine, Shinshu University, Matsumoto, Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Ehime, Department of Rheumatology, Sasebo City General Hospital, Sasebo, Department of Rheumatology, Shinto Kumamoto Hospital, Kumamoto, Clinical Research Center, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Institute of Tropical Medicine (NEKKEN), Nagasaki University and Department of Rheumatology, Nagasaki University Hospital, Nagasaki, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: Department of Rheumatology and Clinical Research Center, Nagasaki Medical Center, Omura, Department of Rheumatology, Isahaya Health Insurance General Hospital, Nagasaki, Department of Infection and Host Defense, Graduate School of Medicine, Shinshu University, Matsumoto, Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Ehime, Department of Rheumatology, Sasebo City General Hospital, Sasebo, Department of Rheumatology, Shinto Kumamoto Hospital, Kumamoto, Clinical Research Center, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Institute of Tropical Medicine (NEKKEN), Nagasaki University and Department of Rheumatology, Nagasaki University Hospital, Nagasaki, Japan.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Iwanaga
authors.affiliation: Department of Rheumatology and Clinical Research Center, Nagasaki Medical Center, Omura, Department of Rheumatology, Isahaya Health Insurance General Hospital, Nagasaki, Department of Infection and Host Defense, Graduate School of Medicine, Shinshu University, Matsumoto, Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Ehime, Department of Rheumatology, Sasebo City General Hospital, Sasebo, Department of Rheumatology, Shinto Kumamoto Hospital, Kumamoto, Clinical Research Center, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Institute of Tropical Medicine (NEKKEN), Nagasaki University and Department of Rheumatology, Nagasaki University Hospital, Nagasaki, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Furukawa
authors.affiliation: Department of Rheumatology and Clinical Research Center, Nagasaki Medical Center, Omura, Department of Rheumatology, Isahaya Health Insurance General Hospital, Nagasaki, Department of Infection and Host Defense, Graduate School of Medicine, Shinshu University, Matsumoto, Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Ehime, Department of Rheumatology, Sasebo City General Hospital, Sasebo, Department of Rheumatology, Shinto Kumamoto Hospital, Kumamoto, Clinical Research Center, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Institute of Tropical Medicine (NEKKEN), Nagasaki University and Department of Rheumatology, Nagasaki University Hospital, Nagasaki, Japan.
authors.forename: Michio
authors.initials: M
authors.lastname: Yasunami
authors.affiliation: Department of Rheumatology and Clinical Research Center, Nagasaki Medical Center, Omura, Department of Rheumatology, Isahaya Health Insurance General Hospital, Nagasaki, Department of Infection and Host Defense, Graduate School of Medicine, Shinshu University, Matsumoto, Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Ehime, Department of Rheumatology, Sasebo City General Hospital, Sasebo, Department of Rheumatology, Shinto Kumamoto Hospital, Kumamoto, Clinical Research Center, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Institute of Tropical Medicine (NEKKEN), Nagasaki University and Department of Rheumatology, Nagasaki University Hospital, Nagasaki, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kawakami
authors.affiliation: Department of Rheumatology and Clinical Research Center, Nagasaki Medical Center, Omura, Department of Rheumatology, Isahaya Health Insurance General Hospital, Nagasaki, Department of Infection and Host Defense, Graduate School of Medicine, Shinshu University, Matsumoto, Department of Pathology, Ehime University Graduate School of Medicine and Proteo-Science Center, Ehime, Department of Rheumatology, Sasebo City General Hospital, Sasebo, Department of Rheumatology, Shinto Kumamoto Hospital, Kumamoto, Clinical Research Center, Sagamihara National Hospital, National Hospital Organization, Sagamihara, Institute of Tropical Medicine (NEKKEN), Nagasaki University and Department of Rheumatology, Nagasaki University Hospital, Nagasaki, Japan.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Eguchi
mesh_terms: D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000378:metabolism; D044466:Asian People; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D010505:Familial Mediterranean Fever / Q000378:metabolism*; D005260:Female; D006801:Humans; D015151:Immunoblotting; D053583:Interleukin-1beta / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D000685:Serum Amyloid A Protein / Q000378:metabolism*
chemical_list: D015415:Biomarkers; C508585:IL1B protein, human; D053583:Interleukin-1beta; D000685:Serum Amyloid A Protein
keywords: IL-1β; autoinflammatory disease; biomarker; familial Mediterranean fever; serum amyloid A