title: One-pot synthesis of protein-embedded metal-organic frameworks with enhanced biological activities.
abstract: Protein molecules were directly embedded in metal-organic frameworks (MOFs) by a coprecipitation method. The protein molecules majorly embedded on the surface region of MOFs display high biological activities. As a demonstration of the power of such materials, the resulting Cyt c embedded in ZIF-8 showed a 10-fold increase in peroxidase activity compared to free Cyt c in solution and thus gave convenient, fast, and highly sensitive detection of trace amounts of explosive organic peroxides in solution.
authors.affiliation: Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University , Beijing 100084, China.
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Lyu
authors.forename: Yifei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Richard N
authors.initials: RN
authors.lastname: Zare
authors.forename: Jun
authors.initials: J
authors.lastname: Ge
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
mesh_terms: D015374:Biosensing Techniques; D045304:Cytochromes c / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D009942:Organometallic Compounds / Q000737:chemistry*; D010545:Peroxides / Q000032:analysis* / Q000378:metabolism
chemical_list: D055767:Immobilized Proteins; D009942:Organometallic Compounds; D010545:Peroxides; D045304:Cytochromes c
keywords: Enzyme; biocatalysis; metal−organic framework; nanomaterials