title: Standardization of solvent extracts from Onopordum acanthium fruits by GC-MS, HPLC-UV/DAD, HPLC-TQMS and 1H-NMR and evaluation of their inhibitory effects on the expression of IL-8 and E-selectin in immortalized endothelial cells (HUVECtert).
abstract: In this work we have characterized and standardized the solvent extracts of the fruits of Onopordum acanthium, a plant widely distributed from Europe to Asia and used in different traditional medicines. Fruits were extracted with methanol (ME) and n-hexane (HE) and the extract compositions determined by GC-MS, HPLC-UV/DAD, HPLC-TQMS and 1H NMR spectroscopy. Anti-inflammatory activity (IL-8 and E-selectin, qPCR and ELISA) was investigated in HUVECtert cells stimulated with TNF-alpha and LPS. Arctiin and isochlorogenic acid were found in ME (87 +/- 2%, w/w, and 10.2 +/- 0.2%, w/w; 38.0 +/- 3.2 mg/gFRUITS and 3.5 +/- 0.4 mg/gFRUITS) and (ii) paraffins in the HE (195.6 +/- 5.6 mg/g). A dose dependent (from 15 to 40 microgME/mL corresponding to 20-75 microM arctiin) inhibition of E-selectin and of the induction of IL-8 was induced by LPS. The results of this study support the use of O. acanthium fruits in traditional medicine as an anti-inflammatory agent and for cancer prevention and treatment.
authors.forename: Armond
authors.initials: A
authors.lastname: Daci
authors.forename: Markus
authors.initials: M
authors.lastname: Gold-Binder
authors.forename: Davide
authors.initials: D
authors.lastname: Garzon
authors.forename: Alessio
authors.initials: A
authors.lastname: Patea
authors.forename: Giangiacomo
authors.initials: G
authors.lastname: Beretta
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019040:E-Selectin / Q000235:genetics / Q000378:metabolism; D005638:Fruit / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D031183:Onopordum / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C508606:CXCL8 protein, human; D019040:E-Selectin; D016209:Interleukin-8; D010936:Plant Extracts