title: Isolation of multiple novel paramyxoviruses from pteropid bat urine.
abstract: Bats have been found to harbour a number of new emerging viruses with zoonotic potential, and there has been a great deal of interest in identifying novel bat pathogens to determine the risk to human and animal health. Many groups have identified novel viruses in bats by detection of viral nucleic acid; however, virus isolation is still a challenge, and there are few reports of viral isolates from bats. In recent years, our group has developed optimized procedures for virus isolation from bat urine, including the use of primary bat cells. In previous reports, we have described the isolation of Hendra virus, Menangle virus and Cedar virus in Queensland, Australia. Here, we report the isolation of four additional novel bat paramyxoviruses from urine collected from beneath pteropid bat (flying fox) colonies in Queensland and New South Wales during 2009-2011.
authors.affiliation: CSIRO Australian Animal Health Laboratory, Geelong, Australia.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Barr
authors.affiliation: Queensland Department of Agriculture, Fisheries and Forestry, Brisbane, Australia.
authors.forename: Craig
authors.initials: C
authors.lastname: Smith
authors.affiliation: CSIRO Australian Animal Health Laboratory, Geelong, Australia.
authors.forename: Ina
authors.initials: I
authors.lastname: Smith
authors.affiliation: Queensland Department of Agriculture, Fisheries and Forestry, Brisbane, Australia.
authors.forename: Carol
authors.initials: C
authors.lastname: de Jong
authors.affiliation: CSIRO Australian Animal Health Laboratory, Geelong, Australia.
authors.forename: Shawn
authors.initials: S
authors.lastname: Todd
authors.affiliation: Queensland Department of Agriculture, Fisheries and Forestry, Brisbane, Australia.
authors.forename: Debra
authors.initials: D
authors.lastname: Melville
authors.affiliation: Queensland Department of Agriculture, Fisheries and Forestry, Brisbane, Australia.
authors.forename: Alice
authors.initials: A
authors.lastname: Broos
authors.affiliation: CSIRO Australian Animal Health Laboratory, Geelong, Australia.
authors.forename: Sandra
authors.initials: S
authors.lastname: Crameri
authors.affiliation: CSIRO Australian Animal Health Laboratory, Geelong, Australia.
authors.forename: Jessica
authors.initials: J
authors.lastname: Haining
authors.affiliation: CSIRO Australian Animal Health Laboratory, Geelong, Australia.
authors.forename: Glenn
authors.initials: G
authors.lastname: Marsh
authors.affiliation: CSIRO Australian Animal Health Laboratory, Geelong, Australia.
authors.forename: Gary
authors.initials: G
authors.lastname: Crameri
authors.affiliation: Ecohealth Alliance, New York, NY, USA.|Queensland Department of Agriculture, Fisheries and Forestry, Brisbane, Australia.
authors.forename: Hume
authors.initials: H
authors.lastname: Field
authors.affiliation: Program in Emerging Infectious Diseases, Duke-NUS Graduate Medical School, Singapore.|CSIRO Australian Animal Health Laboratory, Geelong, Australia.
authors.forename: Lin-Fa
authors.initials: LF
authors.lastname: Wang
mesh_terms: D000818:Animals; D001315:Australia; D002685:Chiroptera / Q000821:virology*; D018184:Paramyxoviridae Infections / Q000821:virology; D018098:Paramyxovirinae / Q000235:genetics* / Q000302:isolation & purification*; D014556:Urine / Q000821:virology*; D015047:Zoonoses / Q000821:virology