title: Prevalence of Escherichia coli sequence type 131 and its H30 subclone among E. coli isolates in a French hospital.
abstract: The prevalence of Escherichia coli sequence type 131 (ST131) and its subclone H30 was assessed among a collection of 490 E. coli isolated in 2013 in a French university hospital. The prevalence of ST131 was 4% among bloodstream isolates (regardless of antimicrobial resistance) and 17.2% among extended-spectrum β-lactamase (ESBL)-producing isolates. Although a much lower prevalence of ST131 was found among bloodstream E. coli isolates compared with other countries, a large predominance of H30 subclone within ST131 was confirmed. It was also confirmed that, among ESBL-producing E. coli, ST131 isolates were more frequently resistant to amoxicillin/clavulanic acid, ceftazidime, fluoroquinolones and aminoglycosides than non-ST131 isolates.
authors.affiliation: Service d'Hygiène Hospitalière, UMR 6249 Chrono-environnement, Centre Hospitalier Régional Universitaire, Université de Franche-Comté, Besançon, France.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Lafolie
authors.affiliation: Service de Microbiologie, Hôpital Beaujon AP-HP, Clichy, France; Faculté de Médecine D. Diderot, Université Paris 7, Paris, France; INSERM UMR 1149, Université Paris 7, Paris, France.
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Nicolas-Chanoine
authors.affiliation: Service de Parasitologie-Mycologie, UMR 6249 Chrono-environnement, Centre Hospitalier Régional Universitaire, Université de Franche-Comté, Besançon, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Grenouillet
authors.affiliation: Service d'Hygiène Hospitalière, UMR 6249 Chrono-environnement, Centre Hospitalier Régional Universitaire, Université de Franche-Comté, Besançon, France; Centre de Ressources Biologiques Ferdinand Cabanne, Centre Hospitalier Régional Universitaire, Besançon, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Hocquet
authors.affiliation: Service d'Hygiène Hospitalière, UMR 6249 Chrono-environnement, Centre Hospitalier Régional Universitaire, Université de Franche-Comté, Besançon, France; Centre de Ressources Biologiques Ferdinand Cabanne, Centre Hospitalier Régional Universitaire, Besançon, France. Electronic address: xbertrand@chu-besancon.fr.
authors.forename: Xavier
authors.initials: X
authors.lastname: Bertrand
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology*; D005602:France / Q000453:epidemiology; D005838:Genotype; D006785:Hospitals, University; D058885:Multilocus Sequence Typing; D015995:Prevalence; D012189:Retrospective Studies; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases
keywords: Clonal expansion; ESBL; MLST; Multidrug resistance; Phylogenetic analysis