title: Glutathione-triggered formation of a Fmoc-protected short peptide-based supramolecular hydrogel.
abstract: A biocompatible method of glutathione (GSH) catalyzed disulfide bond reduction was used to form Fmoc-short peptide-based supramolecular hydrogels. The hydrogels could form in both buffer solution and cell culture medium containing 10% of Fetal Bovine Serum (FBS) within minutes. The hydrogel was characterized by rheology, transmission electron microscopy, and fluorescence emission spectra. Their potential in three dimensional (3D) cell culture was evaluated and the results indicated that the gel with a low concentration of the peptide (0.1 wt%) was suitable for 3D cell culture of 3T3 cells. This study provides an alternative candidate of supramolecular hydrogel for 3D cell culture and cell delivery.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin, P. R. China.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin, P. R. China.
authors.forename: Huaimin
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Chemical and Materials Engineering, Hubei Institute of Technology, Huangshi, Hubei, P. R. China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Hu
authors.affiliation: School of Chemical and Materials Engineering, Hubei Institute of Technology, Huangshi, Hubei, P. R. China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin, P. R. China.
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D018929:Cell Culture Techniques; D020285:Cryoelectron Microscopy; D003470:Culture Media; D005449:Fluorenes / Q000737:chemistry; D005978:Glutathione / Q000737:chemistry*; D020100:Hydrogels / Q000138:chemical synthesis*; D051379:Mice; D041681:NIH 3T3 Cells
chemical_list: D000596:Amino Acids; D003470:Culture Media; D005449:Fluorenes; D020100:Hydrogels; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D005978:Glutathione