title: Identification and molecular characterization of a novel splice variant of human 5- lipoxygenase gene.
abstract: 5-Lipoxygenase (5-LO) is one of the members of Lipoxygenase family. It breaks down arachidonic acid to pro-inflammatory compounds like leukotrienes. Leukotriene plays a major role in the inflammatory process. In this study, while cloning full length 5-LO, a novel splice variant of 5-LO (t5-LO) was found to be expressed in HepG2 cell line. The complete ORF of t5-LO is 420 bp long, expressing 139 amino acid long proteins from N-terminal. The splice variant of 5-LO was cloned, expressed, purified in bacterial system and characterized by MS/MS and western blot experiments. The full length 5-LO is 674 amino acids long encoded by 2,025 bp long ORF. RT-PCR and western blot revealed that t5-LO is extensively expressed in HepG2 cell line.
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110029, India.
authors.forename: Abhay Kumar
authors.initials: AK
authors.lastname: Singh
authors.forename: Rahul
authors.initials: R
authors.lastname: Kumar
authors.forename: Abhay Anand
authors.initials: AA
authors.lastname: Shukla
authors.forename: Gururao
authors.initials: G
authors.lastname: Hariprasad
authors.forename: Shyam Sunder
authors.initials: SS
authors.lastname: Chauhan
authors.forename: Sharmistha
authors.initials: S
authors.lastname: Dey
mesh_terms: D017398:Alternative Splicing; D001094:Arachidonate 5-Lipoxygenase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D020134:Catalytic Domain; D002460:Cell Line; D003001:Cloning, Molecular; D018922:HL-60 Cells; D056945:Hep G2 Cells; D006801:Humans; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D022821:RNA Splice Sites / Q000235:genetics*
chemical_list: D020033:Protein Isoforms; D022821:RNA Splice Sites; D001094:Arachidonate 5-Lipoxygenase; C583212:ALOX5 protein, human