title: Evaluation of the poly(lactic-co-glycolic acid)/pluronic F127 for injection laryngoplasty in rabbits.
abstract: OBJECTIVE
  Poly(lactic-co-glycolic acid) (PLGA) is an aliphatic polyester and one of the most commonly used synthetic biodegradable polymers for tissue engineering. The objectives of this study were to evaluate the biocompatibility of PLGA/Pluronic F127 in the vocal fold.
  
  
  METHODS
  A randomized, prospective, controlled animal study.
  
  
  METHODS
  University laboratory.
  
  
  METHODS
  We used 18 New Zealand white rabbits, which were divided into 5% PLGA solution (n = 9) and 10% PLGA solution (n = 9) groups. The PLGA/Pluronic F127 solutions were injected into the rabbit vocal fold. Laryngoscopic exams were performed at 1, 4, and 8 weeks after implantation; then larynx specimens were sampled. High-speed video camera examination was performed for functional analysis of vocal mucosa vibration at 8 weeks after implantation. Also, we evaluated the amplitude of the mucosal wave from the laryngeal midline on high-speed recording. Histologic study of larynx specimen was performed at 4 and 8 weeks.
  
  
  RESULTS
  All animals survived until the scheduled period. Laryngoscopic analysis showed that both 5% and 10% PLGA/Pluronic F127 maintained after 8 weeks after injection without significant inflammatory response. On functional analysis, high-speed camera examination revealed regular and symmetric contact of vocal fold mucosa without a distorted movement by injected PLGA/Pluronic F127. Histologically, no significant inflammation was observed in the injected vocal fold.
  
  
  CONCLUSIONS
  As a vocal fold injection material, PLGA/Pluronic F127 showed a good bio-compatibility without significant inflammatory response. Further experiment will follow to elucidate its role for drug or gene delivery into the vocal fold.
authors.affiliation: Department of Advanced Materials, Hannam University, Daejeon, Republic of Korea.
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Dong Wook
authors.initials: DW
authors.lastname: Kim
authors.affiliation: Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Songpa-gu, Seoul, Republic of Korea.
authors.forename: Eun Na
authors.initials: EN
authors.lastname: Kim
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Dongguk University Ilsan Hospital, Goyang, Republic of Korea.
authors.forename: Seok-Won
authors.initials: SW
authors.lastname: Park
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Dongguk University Ilsan Hospital, Goyang, Republic of Korea.
authors.forename: Hee-Bok
authors.initials: HB
authors.lastname: Kim
authors.affiliation: Department of Nanobiomedical Science, Dankook University, Cheonan, Republic of Korea.
authors.forename: Se Heang
authors.initials: SH
authors.lastname: Oh
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Republic of Korea Cancer Research Institute, Seoul, Republic of Korea Seoul National University Medical Research Center, Seoul, Republic of Korea otolarynx@hanmail.net.
authors.forename: Seong Keun
authors.initials: SK
authors.lastname: Kwon
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage*; D007267:Injections; D019344:Lactic Acid / Q000008:administration & dosage*; D058753:Laryngoplasty / Q000379:methods*; D008297:Male; D008422:Materials Testing; D020442:Poloxamer / Q000008:administration & dosage*; D011100:Polyglycolic Acid / Q000008:administration & dosage*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D014827:Vocal Cords
chemical_list: D001672:Biocompatible Materials; D020442:Poloxamer; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: Poly(lactic-co-glycolic acid); augmentation; drug delivery system; injection laryngoplasty; vocal fold palsy