title: Triterpenoid saponins from the rhizomes of Anemone flaccida and their inhibitory activities on LPS-induced NO production in macrophage RAW264.7 cells.
abstract: A new ursane-type triterpenoid saponin, flaccidoside IV (1), and three new oleanane-type triterpenoid saponins, flaccidosides V-VII (2-4), along with 17 known saponins (5-21), were isolated from the rhizomes of Anemone flaccida. The structures of the new triterpenoid saponins were determined based on spectroscopic analyses and chemical methods. All the isolated saponins were tested for their inhibitory activities on lipopolysaccharide-induced nitric oxide production in RAW264.7 macrophages, and several bisdesmosidic oleanane-type triterpenoid saponins (2, 7, and 10) showed significant inhibitory activities, which indicated they had potential anti-inflammatory activities under their noncytotoxic concentrations in vitro.
authors.affiliation: a Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University , Guangzhou 510632 , China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Huang
authors.forename: Jing-Qun
authors.initials: JQ
authors.lastname: Tang
authors.forename: Man-Mei
authors.initials: MM
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Yao-Lan
authors.initials: YL
authors.lastname: Li
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Fan
authors.forename: Hong
authors.initials: H
authors.lastname: Pei
authors.forename: Hui-Nan
authors.initials: HN
authors.lastname: Zhao
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Wen-Cai
authors.initials: WC
authors.lastname: Ye
mesh_terms: D031883:Anemone / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D012503:Saponins; D014315:Triterpenes; C000593152:flaccidoside IV; D009569:Nitric Oxide
keywords: Anemone flaccida; RAW264.7 cells; Ranunculaceae; anti-inflammatory; triterpenoid saponin