title: Basal foot MTOC organizes pillar MTs required for coordination of beating cilia.
abstract: Coordination of ciliary beating is essential to ensure mucus clearance in the airway tract. The orientation and synchronization of ciliary motion responds in part to the organization of the underlying cytoskeletal networks. Using electron tomography on mouse trachea, we show that basal bodies are collectively hooked at the cortex by a regular microtubule array composed of 4-5 microtubules. Removal of galectin-3, one of basal-body components, provokes misrecruitment of γ-tubulin, disorganization of this microtubule framework emanating from the basal-foot cap, together with loss of basal-body alignment and cilium orientation, defects in cilium organization and reduced fluid flow in the tracheal lumen. We conclude that galectin-3 plays a crucial role in the maintenance of the microtubule-organizing centre of the cilium and the 'pillar' microtubules, and that this network is instrumental for the coordinated orientation and stabilization of motile cilia.
authors.affiliation: Institute of Structural and Molecular Biology, Birkbeck College and University College of London, Malet Street, London WC1E 7HX, UK.
authors.forename: Daniel K
authors.initials: DK
authors.lastname: Clare
authors.affiliation: Morphogenesis, homeostasis and pathologies, Jacques Monod Institute, CNRS-UMR7592, Paris Diderot University, 15 Rue Hélène Brion, 75205 Paris, Cedex 13, France.
authors.forename: Jérémy
authors.initials: J
authors.lastname: Magescas
authors.affiliation: ImagoSeine, Jacques Monod Institute, CNRS-UMR7592, Paris Diderot University, 15 Rue Hélène Brion, 75205 Paris, Cedex 13, France.
authors.forename: Tristan
authors.initials: T
authors.lastname: Piolot
authors.affiliation: Institute of Structural and Molecular Biology, Birkbeck College and University College of London, Malet Street, London WC1E 7HX, UK.
authors.forename: Maud
authors.initials: M
authors.lastname: Dumoux
authors.affiliation: Laboratoire de Biologie du Développement, INSERM U969-UMR 7622, Pierre and Marie Curie University (UPMC), 9 Quai Saint-Bernard, 75005 Paris, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Vesque
authors.affiliation: Morphogenesis, homeostasis and pathologies, Jacques Monod Institute, CNRS-UMR7592, Paris Diderot University, 15 Rue Hélène Brion, 75205 Paris, Cedex 13, France.
authors.forename: Evelyne
authors.initials: E
authors.lastname: Pichard
authors.affiliation: Morphogenesis, homeostasis and pathologies, Jacques Monod Institute, CNRS-UMR7592, Paris Diderot University, 15 Rue Hélène Brion, 75205 Paris, Cedex 13, France.
authors.forename: Tien
authors.initials: T
authors.lastname: Dang
authors.affiliation: Morphogenesis, homeostasis and pathologies, Jacques Monod Institute, CNRS-UMR7592, Paris Diderot University, 15 Rue Hélène Brion, 75205 Paris, Cedex 13, France.
authors.forename: Boris
authors.initials: B
authors.lastname: Duvauchelle
authors.affiliation: Morphogenesis, homeostasis and pathologies, Jacques Monod Institute, CNRS-UMR7592, Paris Diderot University, 15 Rue Hélène Brion, 75205 Paris, Cedex 13, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Poirier
authors.affiliation: Morphogenesis, homeostasis and pathologies, Jacques Monod Institute, CNRS-UMR7592, Paris Diderot University, 15 Rue Hélène Brion, 75205 Paris, Cedex 13, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Delacour
mesh_terms: D000818:Animals; D002923:Cilia / Q000378:metabolism / Q000648:ultrastructure*; D037502:Galectin 3 / Q000172:deficiency / Q000235:genetics*; D015870:Gene Expression; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008854:Microscopy, Electron; D022101:Microtubule-Organizing Center / Q000378:metabolism / Q000648:ultrastructure*; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure*; D020545:Respiratory Mucosa / Q000378:metabolism / Q000648:ultrastructure*; D012212:Rheology; D014132:Trachea / Q000378:metabolism / Q000648:ultrastructure*; D014404:Tubulin / Q000235:genetics / Q000378:metabolism
chemical_list: D037502:Galectin 3; C485095:Lgals3 protein, mouse; D014404:Tubulin