title: Novel dammarane saponins from Gynostemma pentaphyllum and their cytotoxic activities against HepG2 cells.
abstract: Two new dammarane saponins, 2α,3β,12β-trihydroxydammar-20(22),24-diene-3-O-[β-D-glucopyranoxyl(1→2)-β-D-6″-O-acetylglucopyranoside (1, namely damulin C) and 2α,3β,12β-trihydroxydammar-20(21),24-diene-3-O-[β-D-glucopyranoxyl(1→2)-β-D-6″-O-acetylglucopyranoside (2, namely damulin D), were isolated from the ethanol extract of Gynostemma pentaphyllum, which had been heat processed by steaming at 125 °C. The NMR spectroscopic data of the novel saponins were completely assigned by using a combination of 2D NMR experiments including (1)H-(1)H COSY, HSQC, and HMBC. Their cytotoxic activities of human liver adenocarcinoma HepG2 cells were evaluated in vitro. They showed cytotoxicities against HepG2 cell line with IC50 of 40±0.7 and 38±0.5 μg/ml, respectively.
authors.affiliation: Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China. Electronic address: xlpiao@163.com.
authors.forename: Xiang-Lan
authors.initials: XL
authors.lastname: Piao
authors.affiliation: Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China.
authors.forename: Shao-Fang
authors.initials: SF
authors.lastname: Xing
authors.affiliation: Laboratory of Comparative Medicine, Guangdong Medical Laboratory Animal Center, Foshan, Guangdong 528248, China.
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Lou
authors.affiliation: Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China.
authors.forename: Dao-Jin
authors.initials: DJ
authors.lastname: Chen
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D029774:Gynostemma / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D015394:Molecular Structure; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000097669:Dammaranes
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D012503:Saponins; D014315:Triterpenes; D000097669:Dammaranes
keywords: Dammarane saponin; Damulin C; Damulin D; Gynostemma pentaphyllum; HepG2 cell cytotoxic activity