title: Effect of dexamethasone, insulin and EGF on the myogenic potential on human endometrial stem cell.
abstract: Human endometrium contains mesenchymal stem cells (eMSC) which have the ability to differentiate into three cell lineages and the potential in therapeutic applications. We hypothesize that using environmental induction in culture media such as dexamethasone, human recombinant insulin and human epidermal growth factor (hEGF) can differentiate endometrial stem cells into myoblast. These agents have a broad range of effects in myoblast differentiation in-vitro. We used immunohystochemistry analysis and RT -PCR to evaluate the presence of skeletal muscle - specific proteins some of which are expressed in the early stage of differentiation including myoD and Desmin which expressed at later stages of differentiation. In conclusion eMSC can differentiate in culture media which contains above mentioned factors and use for therapeutic purpose in muscular degenerative disease.
authors.affiliation: Depatment of Biology, Science and Research Branch Islamic Azad University Tehran Iran.
authors.forename: Hora
authors.initials: H
authors.lastname: Jalali Tehrani
authors.affiliation: Depatment of Biology, Science and Research Branch Islamic Azad University Tehran Iran.
authors.forename: Kazem
authors.initials: K
authors.lastname: Parivar
authors.affiliation: Department of Tissue Engineering, Faculty of Advance Technology, University of Medical Sciences, Tehran, Iran.
authors.forename: Jafar
authors.initials: J
authors.lastname: Ai
authors.affiliation: Paediatric Urology Research Center, Department of Urology, Children's Hospital Medical Center, Tehran University of Medical Sciences,Tehran, Iran.
authors.forename: Abdolmohammad
authors.initials: A
authors.lastname: Kajbafzadeh
authors.affiliation: Umbilical Cord Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Rahbarghazi
authors.affiliation: Department of Medical Genetics. Tehran Medical Sciences Branch .Islamic Azad University Tehran, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Hashemi
authors.affiliation: Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Sadeghizadeh
keywords: Dexamethasone; Differentiation; Endometrial stem cell; Myogenic potential