title: Pathogen-induced SGT1 of Arachis diogoi induces cell death and enhanced disease resistance in tobacco and peanut.
abstract: We have identified a transcript derived fragment (TDF) corresponding to SGT1 in a study of differential gene expression on the resistant wild peanut, Arachis diogoi, upon challenge from the late leaf spot pathogen, Phaeoisariopsis personata, and cloned its full-length cDNA followed by subsequent validation through q-PCR. Sodium nitroprusside, salicylic acid, ethephon and methyl jasmonate induced the expression of AdSGT1, while the treatment with abscisic acid did not elicit its up-regulation. AdSGT1 is localized to both nucleus and cytoplasm. Its overexpression induced hypersensitive-like cell death in tobacco under transient conditional expression using the estradiol system, and this conditional expression of AdSGT1 was also associated with the up-regulation of NtHSR203J, HMGR and HIN1, which have been shown to be associated with hypersensitive response in tobacco in earlier studies. Expression of the cDNA in a susceptible cultivated peanut variety enhanced its resistance against the late leaf spot pathogen, Phaeoisariopsis personata, while the heterologous expression in tobacco enhanced its resistance against Phytophthora parasitica var. nicotianae, Alternaria alternata var. nicotianae and Rhizoctonia solani. Constitutive expression in peanut was associated with the co-expression of resistance-related genes, CC-NB-LRR and some protein kinases.
authors.affiliation: Department of Plant Sciences, University of Hyderabad, Hyderabad, India.
authors.forename: Dilip
authors.initials: D
authors.lastname: Kumar
authors.forename: Pulugurtha Bharadwaja
authors.initials: PB
authors.lastname: Kirti
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D010367:Arachis / Q000166:cytology* / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D016923:Cell Death; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000302:isolation & purification; D060467:Disease Resistance; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D010838:Phytophthora / Q000502:physiology; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D021381:Protein Transport; D060888:Real-Time Polymerase Chain Reaction; D012232:Rhizoctonia / Q000502:physiology; D015398:Signal Transduction; D013347:Subcellular Fractions / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology* / Q000235:genetics / Q000382:microbiology*; D014170:Transformation, Genetic; D019076:Transgenes
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins
keywords: AdSGT1; hypersensitive-like cell death; late leaf spot; tobacco; transgenic peanut