title: Inhibitory effect of Lavandula viridis on Fe(2+)-induced lipid peroxidation, antioxidant and anti-cholinesterase properties.
abstract: In this research, the total phenolic content of a Lavandula viridis methanol extract was evaluated and the phenolic identification and quantification was assessed. Rosmarinic acid and luteolin-7-O-glucoside were the two major compounds identified by HPLC-DAD (ca. 39 and 13g/kg, respectively). This extract showed a strong antioxidant activity in ORAC (2858.39±70.97μmolTE/gextract) and TEAC (967.18±22.57μmolTE/gextract) assays, as well as Fe(2+) chelating and OH scavenging abilities. Furthermore, the extract prevented Fe(2+)-induced lipid peroxidation, by reducing MDA content in mouse brains (in vitro), and inhibited AChE and BChE activities both in vitro and in vivo. These findings demonstrate that the methanol extract from L. viridis is a potential source of natural antioxidants and cholinesterase inhibitors.
authors.affiliation: Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology (IBB-CGB), Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, Ed. 8, 8005-139 Faro, Portugal.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Costa
authors.affiliation: Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology (IBB-CGB), Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, Ed. 8, 8005-139 Faro, Portugal.
authors.forename: Sandra
authors.initials: S
authors.lastname: Gonçalves
authors.affiliation: REQUIMTE/Laboratório de Farmacognosia, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha, 164, 4050-047 Porto, Portugal.
authors.forename: Paula B
authors.initials: PB
authors.lastname: Andrade
authors.affiliation: REQUIMTE/Laboratório de Farmacognosia, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha, 164, 4050-047 Porto, Portugal.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valentão
authors.affiliation: Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology (IBB-CGB), Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, Ed. 8, 8005-139 Faro, Portugal. Electronic address: aromano@ualg.pt.
authors.forename: Anabela
authors.initials: A
authors.lastname: Romano
keywords: Alzheimer’s disease; Anti-cholinesterase; Antioxidants; Fe(2+) chelation; Free radicals; Lipid peroxidation