title: Investigation of antibacterial activity of Bacillus spp. isolated from the feces of Giant Panda and characterization of their antimicrobial gene distributions.
abstract: Bacillus group is a prevalent community of Giant Panda's intestinal flora, and plays a significant role in the field of biological control of pathogens. To understand the diversity of Bacillus group from the Giant Panda intestine and their functions in maintaining the balance of the intestinal microflora of Giant Panda, this study isolated a significant number of strains of Bacillus spp. from the feces of Giant Panda, compared the inhibitory effects of these strains on three common enteric pathogens, investigated the distributions of six universal antimicrobial genes (ituA, hag, tasA, sfp, spaS and mrsA) found within the Bacillus group by PCR, and analyzed the characterization of antimicrobial gene distributions in these strains using statistical methods. The results suggest that 34 strains of Bacillus spp. were isolated which has not previously been detected at such a scale, these Bacillus strains could be classified into five categories as well as an external strain by 16S rRNA; Most of Bacillus strains are able to inhibit enteric pathogens, and the antimicrobial abilities may be correlated to their categories of 16S rRNA; The detection rates of six common antimicrobial genes are between 20.58 %(7/34) and 79.41 %(27/34), and genes distribute in three clusters in these strains. We found that the antimicrobial abilities of Bacillus strains can be one of the mechanisms by which Giant Panda maintains its intestinal microflora balance, and may be correlated to their phylogeny.
authors.affiliation: The Key Laboratory of Animal Disease and Human Health of Sichuan Province/College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, 625014, People's Republic of China, zhouziyao988@gmail.com.
authors.forename: Ziyao
authors.initials: Z
authors.lastname: Zhou
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Zhou
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Zhong
authors.forename: Chengdong
authors.initials: C
authors.lastname: Wang
authors.forename: Hemin
authors.initials: H
authors.lastname: Zhang
authors.forename: Desheng
authors.initials: D
authors.lastname: Li
authors.forename: Tingmei
authors.initials: T
authors.lastname: He
authors.forename: Caiwu
authors.initials: C
authors.lastname: Li
authors.forename: Xuehan
authors.initials: X
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Yuan
authors.forename: Hanli
authors.initials: H
authors.lastname: Ji
authors.forename: Yongjiu
authors.initials: Y
authors.lastname: Luo
authors.forename: Wuyang
authors.initials: W
authors.lastname: Gu
authors.forename: Hualin
authors.initials: H
authors.lastname: Fu
authors.forename: Guangneng
authors.initials: G
authors.lastname: Peng
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000378:metabolism*; D000898:Antibiosis; D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D005798:Genes, Bacterial; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D001503:Ursidae
chemical_list: D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S