title: Rapid characterization and determination of multiple components in Bu-Shen-Yi-Qi-Fang by high-performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry.
abstract: In this study, a qualitative and quantitative analysis using high-performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry was performed for the quality control of Bu-Shen-Yi-Qi-Fang, a traditional Chinese formula used for asthma. Thirty-four compounds, including flavonoids, isoflavonoids, triterpenoid saponins, and iridoid glycosides were identified or tentatively characterized by comparing their retention times and mass spectra with those of authentic standards or literature data. Sixteen components were considered as the main bioactive constituents of Bu-Shen-Yi-Qi-Fang and they were chosen as the chemical markers in quantitative analysis, including catalpol, leonuride, calycosin-7-O-β-d-glucoside, hyperoside, acteoside, formononetin-7-O-β-D-glucoside, epimedin A, calycosin, icariin, epimedin B, epimedin C, formononetin, astragaloside IV, astragaloside II, baohuoside-I, and astragaloside I. The total run time was 20 min. It was found that the calibration curves for all analytes showed good linearity (R(2) > 0.99) within the test ranges. The relative standard deviations for intra- and inter-day precisions were below 3.9 and 11.7%, respectively. The accuracy was evaluated by the recovery test within the range of 89.20-110.71% with the relative standard deviation < 4.8%. The sample was stable for at least 48 h at 4°C. The results showed that the new approach was effective for the quality control of Bu-Shen-Yi-Qi-Fang.
authors.affiliation: Department of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, China; Xinjiang Uygur Medical College, Hotan, Xinjiang, China.
authors.forename: Mammat
authors.initials: M
authors.lastname: Nurahmat
authors.forename: Meixia
authors.initials: M
authors.lastname: Chen
authors.forename: Qingli
authors.initials: Q
authors.lastname: Luo
authors.forename: Yun
authors.initials: Y
authors.lastname: Ling
authors.forename: Jingcheng
authors.initials: J
authors.lastname: Dong
authors.forename: Chenggang
authors.initials: C
authors.lastname: Huang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D011786:Quality Control; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Bu-Shen-Yi-Qi-Fang; High-Performance Liquid Chromatography; Mass Spectrometry; Rapid Characterization; Traditional Chinese Medicine