title: 20(s)-ginsenoside Rg3-loaded magnetic human serum albumin nanospheres applied to HeLa cervical cancer cells in vitro.
abstract: 20(s)-ginsenoside Rg3 is extracted from traditional Chinese medicine, red ginseng. However, due to its poor aqueous solubility and low oral bioavailability, the use of 20(s)-Rg3 is limited. This study aimed to explore a method of preparing nano-sized 20(s)-ginsenoside Rg3 particle named 20(s)-ginsenoside Rg3-loaded magnetic human serum albumin nanospheres (20(s)-Rg3/HSAMNP) to change dosage form to improve its aqueous solubility and bioavailability. 20(s)-Rg3/HSAMNP were prepared by the desolvation-crosslinking method. The character of 20(s)-Rg3/HSAMNP was detected. An antiproliferative effect and cell apoptosis rates of 20(s)-Rg3/HSAMNP on human cervical cancer cells were determined by the MTT assay and flow cytometry, respectively. TEM analysis showed that 20(s)-Rg3/HSAMNP were approximately spherical and uniform in size. Thermodynamic testing showed that the corresponding magnetic fluid of a specific concentration rosed to a steady temperature of 42-65○C. Iron content was approximately 3 mg/mL. Drug encapsulation efficiency was approximately 70%. The potential of 20(s)-Rg3/HSAMNP combined with magnetic hyperthermia therapy to inhibit cell growth and induce apoptosis was much more prominent than that of the other groups. A new dosage form of 20(s)-Rg3 was prepared, which effectively induced apoptosis in HeLa cervical cancer cells in vitro when combined with hyperthermia.
authors.affiliation: School of Medicine, Southeast University, Nanjing 210009, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Obstetrics, Affiliated Wuxi Maternity and Child Health Care Hospital, Nanjing Medical University, Wuxi 214002, Jiangsu Province, China.
authors.forename: Daozhen
authors.initials: D
authors.lastname: Chen
authors.affiliation: School of Medicine, Southeast University, Nanjing 210009, China.
authors.forename: Mengfei
authors.initials: M
authors.lastname: Li
authors.affiliation: School of Medicine, Southeast University, Nanjing 210009, China.
authors.forename: Fengqin
authors.initials: F
authors.lastname: Miao
authors.affiliation: School of Medicine, Southeast University, Nanjing 210009, China.
authors.forename: Peidang
authors.initials: P
authors.lastname: Liu
authors.affiliation: School of Medicine, Southeast University, Nanjing 210009, China.
authors.forename: Qiusha
authors.initials: Q
authors.lastname: Tang
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D036145:Ginsenosides / Q000008:administration & dosage* / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D058185:Magnetite Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D054118:Nanospheres / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D012709:Serum Albumin / Q000737:chemistry*; D016896:Treatment Outcome
chemical_list: D036145:Ginsenosides; D058185:Magnetite Nanoparticles; D053769:Nanocapsules; D012709:Serum Albumin; C097367:ginsenoside Rg3
keywords: 20(s)-ginsenoside Rg3; Magnetic nanoparticles; cervical cancer cells; human serum albumin nanospheres