title: The role of integrin αv in proliferation and differentiation of human dental pulp cell response to calcium silicate cement.
abstract: BACKGROUND
  It has been proved that integrin αv activity is related to cell proliferation, differentiation, migration, and organ development. However, the biological functions of integrin αv in human dental pulp cells (hDPCs) cultured on silicate-based materials have not been explored. The aim of this study was to investigate the role of integrin αv in the proliferation and odontogenic differentiation of hDPCs cultured with the effect of calcium silicate (CS) cement and β-tricalcium phosphate (TCP) cement.
  
  
  METHODS
  In this study, hDPCs were cultured on CS and TCP materials, and we evaluated fibronectin (FN) secretion and integrin αv expression during the cell attachment stage. After small interfering RNA transfection targeting integrin αv, the proliferation and odontogenesis differentiation behavior of hDPCs were analyzed.
  
  
  RESULTS
  The results indicate that CS releases Si ion-increased FN secretion and adsorption, which promote cell attachment more effectively than TCP. The CS cement facilitates FN and αv subintegrin expression. However, the FN adsorption and integrin expression of TCP are similar to that observed in the control dish. Integrin αv small interfering RNA inhibited odontogenic differentiation of hDPCs with the decreased formation of mineralized nodules on CS. It also down-regulated the protein expression of multiple markers of odontogenesis and the expression of dentin sialophosphoprotein protein.
  
  
  CONCLUSIONS
  These results establish composition-dependent differences in integrin binding and its effectiveness as a mechanism regulating cellular responses to biomaterial surface.
authors.affiliation: School of Dentistry, Chung Shan Medical University, Taichung City, Taiwan; Department of Stomatology, Chung Shan University Hospital, Taichung City, Taiwan.
authors.forename: Chi-Jr
authors.initials: CJ
authors.lastname: Hung
authors.affiliation: Institute of Oral Science, Chung Shan Medical University, Taichung City, Taiwan.
authors.forename: Hsin-I
authors.initials: HI
authors.lastname: Hsu
authors.affiliation: Department of Chemical and Materials Engineering, Tunghai University, Taichung City, Taiwan.
authors.forename: Chi-Chang
authors.initials: CC
authors.lastname: Lin
authors.affiliation: School of Dentistry, Chung Shan Medical University, Taichung City, Taiwan; Department of Stomatology, Chung Shan University Hospital, Taichung City, Taiwan.
authors.forename: Tsui-Hsien
authors.initials: TH
authors.lastname: Huang
authors.affiliation: School of Dentistry, Chung Shan Medical University, Taichung City, Taiwan; Department of Stomatology, Chung Shan University Hospital, Taichung City, Taiwan.
authors.forename: Buor-Chang
authors.initials: BC
authors.lastname: Wu
authors.affiliation: School of Dentistry, Chung Shan Medical University, Taichung City, Taiwan; Department of Stomatology, Chung Shan University Hospital, Taichung City, Taiwan. Electronic address: ctk@csmu.edu.tw.
authors.forename: Chia-Tze
authors.initials: CT
authors.lastname: Kao
authors.affiliation: School of Dentistry, Chung Shan Medical University, Taichung City, Taiwan. Electronic address: eviltacasi@gmail.com.
authors.forename: Ming-You
authors.initials: MY
authors.lastname: Shie
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000494:pharmacology; D002113:Calcification, Physiologic / Q000187:drug effects; D017610:Calcium Compounds / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003738:Dental Cements / Q000494:pharmacology; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects; D016326:Extracellular Matrix Proteins / Q000187:drug effects; D005353:Fibronectins / Q000032:analysis / Q000187:drug effects; D055785:Gene Knockdown Techniques; D006801:Humans; D039564:Integrin alphaV / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D009805:Odontogenesis / Q000187:drug effects; D010750:Phosphoproteins / Q000187:drug effects; D034741:RNA, Small Interfering / Q000235:genetics; D012795:Sialoglycoproteins / Q000187:drug effects; D012823:Silicate Cement / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*; D014162:Transfection; D000099311:Dentin Sialophosphoprotein
chemical_list: D001672:Biocompatible Materials; D017610:Calcium Compounds; D002130:Calcium Phosphates; D003738:Dental Cements; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D039564:Integrin alphaV; D010750:Phosphoproteins; D034741:RNA, Small Interfering; D012795:Sialoglycoproteins; D012823:Silicate Cement; D017640:Silicates; D000099311:Dentin Sialophosphoprotein; C513309:DMP1 protein, human; C485817:beta-tricalcium phosphate; C031293:calcium silicate
keywords: Calcium silicate cement; fibronectin; human dental pulp cell; integrin αv; odontogenesis