title: Formation and working mechanism of the picornavirus VPg uridylylation complex.
abstract: The initiation of picornavirus replication is featured by the uridylylation of viral protein genome-linked (VPg). In this process, viral RNA-dependent RNA polymerase (RdRp) catalyzes two uridine monophosphate (UMP) molecules to the hydroxyl group of the third tyrosine residue of VPg. Subsequently, the uridylylated VPg (VPg-pUpU) functions as the protein primer to initiate the replication of the viral genome. Although a large body of functional and structural works has been performed to define individual snapshots for particular stages of the VPg uridylylation process, the formation, dynamics and mechanism of the whole VPg uridylylation complex still requires further elucidation. We would like to provide an overview of the current knowledge of the picornaviral VPg uridylylation complex in this paper.
authors.affiliation: National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing 100101, China; School of Medicine and MOE Key Laboratory of Protein Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Yuna
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Pharmacy and State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Guo
authors.affiliation: School of Medicine and MOE Key Laboratory of Protein Sciences, Tsinghua University, Beijing 100084, China; Collaborative Innovation Center for Biotherapy, Tsinghua University, Beijing 100084, China; Collaborative Innovation Center for Biotherapy, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, China. Electronic address: louzy@mail.tsinghua.edu.cn.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Lou
mesh_terms: D010849:Picornaviridae / Q000502:physiology*; D055503:Protein Multimerization; D011499:Protein Processing, Post-Translational; D012367:RNA, Viral / Q000096:biosynthesis*; D014542:Uridine Monophosphate / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; D014542:Uridine Monophosphate