title: Genetic analysis of mecA gene and detection of homologue pbpD in Stahylococcus sciuri group.
abstract: Oxacillin/methicillin-resistance is related to the mecA and its regulatory genes mecR1 and mecI. Its origin is still unknown, although evidences support that it is related to CNS, once mecA and a homologue gene, pbpD, were both detected in Staphylococcus sciuri species group. The present work evaluated 210 samples of skin and ear swabs from rodents and 60 nasal swabs from equines of Army Biologic Institute, Rio de Janeiro. Pheno- and genotypic characterization provided 59.52% (25/42) and 78.57% (11/14) S. lentus and S. sciuri, respectively. It was observed that although all S. sciuri isolates tested positive for pbpD, there was no correlation with oxacillin-resistance. On the other hand, isolates tested positive for mecA gene also presented phenotypic oxacillin-resistance in at least one assay. The alignment of the mecA gene showed that the nucleotide sequences were sorted into 2 different groups, one comprising the bovine strains and the other containing human and equine strains.
authors.affiliation: Departamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
authors.forename: Amanda C
authors.initials: AC
authors.lastname: Calazans-Silva
authors.affiliation: Departamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
authors.forename: Pedro T C
authors.initials: PT
authors.lastname: Medeiros
authors.affiliation: Departamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
authors.forename: Dayanne M
authors.initials: DM
authors.lastname: Araujo
authors.affiliation: Departamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
authors.forename: Bruno O
authors.initials: BO
authors.lastname: Carvalho
authors.affiliation: Departamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
authors.forename: Irene S
authors.initials: IS
authors.lastname: Coelho
authors.affiliation: Departamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
authors.forename: Shana M O
authors.initials: SM
authors.lastname: Coelho
authors.affiliation: Departamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
authors.forename: Miliane M S
authors.initials: MM
authors.lastname: Souza
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004423:Ear / Q000382:microbiology; D005798:Genes, Bacterial; D006736:Horses; D009297:Nasal Mucosa / Q000382:microbiology; D010068:Oxacillin / Q000494:pharmacology; D046915:Penicillin-Binding Proteins / Q000235:genetics*; D012377:Rodentia; D012867:Skin / Q000382:microbiology; D013210:Staphylococcus / Q000235:genetics* / Q000302:isolation & purification; D018440:beta-Lactam Resistance
chemical_list: D000900:Anti-Bacterial Agents; D046915:Penicillin-Binding Proteins; D010068:Oxacillin
keywords: Staphylococcus sciuri; antimicrobial resistance; mecA; pbpD