title: A novel role of alkaline phosphatase in the ERK1/2 dephosphorylation in renal cell carcinoma cell lines: a new plausible therapeutic target.
abstract: Extracellular regulated kinase 1/2 (ERK1/2) has been shown to be activated in renal cell carcinoma (RCC). Previously, we have reported aberrant expression/activity of Liver/Bone/Kidney alkaline phosphatase (L/B/K ALP) in RCC. The present study was conducted to find out whether L/B/K ALP has any role in the dephosphorylation of ERK1/2 in renal cancer cell lines. Two renal cancer cell lines viz. ACHN and A498 were transfected with full length L/B/K ALP cDNA. ALP expression/activity and ERK1/2 phosphorylation were evaluated. Increased L/B/K ALP expression/activity was associated with significantly reduced (P = 0.001) phosphorylation status of ERK1/2 in ALP cDNA transfected cells in comparison to that of control. This is the first study that suggests deactivation of ERK1/2 by stimulation of ALP in renal cancer cell lines which can be used as a therapeutic target of RCC.
authors.affiliation: Department of Biochemistry, PGIMER, Chandigarh, India.
authors.forename: Ujjawal
authors.initials: U
authors.lastname: Sharma
authors.affiliation: Department of Biochemistry, PGIMER, Chandigarh, India.
authors.forename: Deeksha
authors.initials: D
authors.lastname: Pal
authors.affiliation: Department of Biochemistry, PGIMER, Chandigarh, India. Electronic address: fateh1977@yahoo.com.
authors.forename: Rajendra
authors.initials: R
authors.lastname: Prasad
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism*; D002292:Carcinoma, Renal Cell / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D018076:DNA, Complementary; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism* / Q000473:pathology; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D058990:Molecular Targeted Therapy; D010766:Phosphorylation; D014162:Transfection
chemical_list: D018076:DNA, Complementary; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D000469:Alkaline Phosphatase
keywords: ERK1/2; L/B/K alkaline phosphatase; Phosphorylation; Transfection