title: LC-MS metabolite fingerprinting and MtSK-based screening of an endophyte Bartalinia pondoensis Marinc of Citrus aurantum L.
abstract: An endophyte Bartalinia pondoensis Marinc of Citrus aurantum L. var. dulcis was isolated and studied for its secondary metabolites and for their Mycobacterium tuberculosis shikimate kinase (MtSK) inhibitory activities. Using LC-MS metabolite fingerprinting of the constituents of the methanol extract, 19 compounds pertaining to various classes were identified: amino acids, proto-alkaloids, fatty acid amides and oxazole, aniline derivatives and aromatic compounds. We report here for the first time the presence of the [N-(ethyloxy, hydroxymethyl)phenylethylamine] as a new proto-alkaloid and 18 other known compounds are reported for the first time in the genus of Bartalinia. MtSK inhibitory activities of methanol extract and fractions obtained by solid phase extraction (SPE) at a concentration of 50 μg/mL may be attributed to the presence of aniline and oxazole derivatives present in all fractions in varying concentrations.
authors.affiliation: Department of Drug Development and Discovery, Harrison School of Pharmacy, Auburn University, 4306 Walker Building, Auburn, AL 36849, USA; Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Zaher
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt.
authors.forename: Makboul A
authors.initials: MA
authors.lastname: Makboul
authors.affiliation: Mycology Center, Faculty of Science, Assiut University, Assiut, Egypt.
authors.forename: Ahmad M
authors.initials: AM
authors.lastname: Moharram
authors.affiliation: Department of Drug Development and Discovery, Harrison School of Pharmacy, Auburn University, 4306 Walker Building, Auburn, AL 36849, USA. Electronic address: aic0001@auburn.edu.
authors.forename: Angela I
authors.initials: AI
authors.lastname: Calderón
mesh_terms: D001203:Ascomycota / Q000737:chemistry*; D002853:Chromatography, Liquid; D002957:Citrus / Q000382:microbiology*; D060026:Endophytes / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000032:analysis; D055442:Metabolome / Q000502:physiology*; D009169:Mycobacterium tuberculosis / Q000201:enzymology; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000037:antagonists & inhibitors* / Q000378:metabolism; D052616:Solid Phase Extraction
chemical_list: D004791:Enzyme Inhibitors; D017853:Phosphotransferases (Alcohol Group Acceptor); C022642:shikimate kinase
keywords: Bartalinia pondoensis; Endophyte; LC–MS; Metabolic fingerprinting