title: Enrichment and optimization of anaerobic bacterial mixed culture for conversion of syngas to ethanol.
abstract: The main aim of the present study was to enrich anaerobic mixed bacterial culture capable of producing ethanol from synthesis gas fermentation. Screening of thirteen anaerobic strains together with enrichment protocol helped to develop an efficient mixed culture capable of utilizing syngas for ethanol production. Physiological and operational parameters were optimized for enhanced ethanol production. The optimized value of operational parameters i.e. initial media pH, incubation temperature, initial syngas pressure, and agitation speed were 6.0±0.1, 37°C, 2kgcm(-2) and 100rpm respectively. Under these conditions ethanol and acetic acid production by the selected mixed culture were 1.54gL(-1) and 0.8gL(-1) respectively. Furthermore, up-scaling studies in semi-continuous fermentation mode further enhanced ethanol and acetic acid production up to 2.2gL(-1) and 0.9gL(-1) respectively. Mixed culture TERI SA1 was efficient for ethanol production by syngas fermentation.
authors.affiliation: TERI University, 10 Institutional Area, Vasant Kunj, New Delhi 110 070, India; TERI, Darbari Seth Block, India Habitat Centre, New Delhi 110 003, India.
authors.forename: Ashish
authors.initials: A
authors.lastname: Singla
authors.affiliation: TERI, Darbari Seth Block, India Habitat Centre, New Delhi 110 003, India.
authors.forename: Dipti
authors.initials: D
authors.lastname: Verma
authors.affiliation: TERI University, 10 Institutional Area, Vasant Kunj, New Delhi 110 070, India; TERI, Darbari Seth Block, India Habitat Centre, New Delhi 110 003, India.
authors.forename: Banwari
authors.initials: B
authors.lastname: Lal
authors.affiliation: TERI University, 10 Institutional Area, Vasant Kunj, New Delhi 110 070, India; TERI, Darbari Seth Block, India Habitat Centre, New Delhi 110 003, India. Electronic address: priyanms@teri.res.in.
authors.forename: Priyangshu M
authors.initials: PM
authors.lastname: Sarma
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D000693:Anaerobiosis; D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D018929:Cell Culture Techniques; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005740:Gases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D011312:Pressure; D013696:Temperature
chemical_list: D005740:Gases; D000431:Ethanol; D019342:Acetic Acid
keywords: Ethanol; Mixed culture; Optimization; Syngas; Up-scaling