title: Temporal Changes in Microbial Metabolic Characteristics in Field-Scale Biopiles Composed of Aged Oil Sludge.
abstract: Disposal of oil sludge, a hazardous waste, is currently a prevalent environmental issue. In this study, two field-scale biopiles were constructed to explore the temporal changes of microbial metabolic characteristics during the biotreatment of aged oil sludge. Bulking agent was mixed thoroughly with oily sludge to form a treated pile. The BIOLOG™ system was used to analyze the community level physiological parameters, including microbial metabolic activity, diversity, and variance. In comparison with the control, the community level physiological parameters of the treated pile were dramatically improved. Microbial metabolic activity of the treated pile was improved by 25.06% calculated from the maximums during the treatment. Microbial diversity index (Shannon index) ranges were improved from 1.64-3.02 (control pile) to 2.34-3.14 (treated pile). The numbers of petroleum-degrading bacteria and the total heterotrophic bacteria were correlated with the environmental temperature, and microbial metabolic characteristics in the treated pile revealed the distinctive carbon resources selection with the addition of cotton stalk. Temporal microbial metabolic characteristics, which have important effect on bioremediation, were revealed in this study.
authors.affiliation: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences , Beijing, China . ; Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology , Chinese Academy of Sciences, Chengdu, China .
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences , Beijing, China .
authors.forename: Fasheng
authors.initials: F
authors.lastname: Li
authors.affiliation: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences , Beijing, China .
authors.forename: Guanlin
authors.initials: G
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences , Beijing, China .
authors.forename: Shijie
authors.initials: S
authors.lastname: Wang
authors.affiliation: Institute of Biochemistry and Physiology of Microorganisms , Russia Academy of Sciences, Pushchino, Russia .
authors.forename: Alexander
authors.initials: A
authors.lastname: Boronin
authors.affiliation: School of Civil and Environmental Engineering, University of Science and Technology Beijing , Beijing, China .
authors.forename: Qunhui
authors.initials: Q
authors.lastname: Wang
keywords: aged oil sludge; bioaugmentation; biopile; community level physiological profiles; temporal change