title: Glucose biosensing using glassy carbon electrode modified with polyhydroxy-C60, glucose oxidase and ionic-liquid.
abstract: Direct electrochemistry of glucose oxidase (GOD) was achieved when an ionic liquid/GOD-Polyhydroxy-C60 functional membrane was confined on a glassy carbon electrode (GCE). The cyclic voltammograms (CVs) of the modified GCE showed a pair of redox peaks with a formal potential (E°') of - 329 ± 2 mV. The heterogeneous electron transfer constant (k(s)) was 1.43 s-1. The modified GCE response to glucose was linear in the range from 0.02 to 2.0 mM. The detection limit was 1 μM. The apparent Michaelis-Menten constant (K(m)(app)) was 1.45 mM.
authors.affiliation: School of Life Sciences, Henan University, Kaifeng 475000, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Yang
authors.affiliation: School of Life Sciences, Henan University, Kaifeng 475000, China.
authors.forename: Xiao-Lu
authors.initials: XL
authors.lastname: Yang
authors.affiliation: School of Life Sciences, Henan University, Kaifeng 475000, China.
authors.forename: Yu-Shuai
authors.initials: YS
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Henan University, Kaifeng 475000, China.
authors.forename: BaoLin
authors.initials: B
authors.lastname: Xiao
authors.affiliation: School of Life Sciences, Henan University, Kaifeng 475000, China Institute of Biotechnology, Henan University, Kaifeng 475000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hong
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D002244:Carbon / Q000737:chemistry; D002264:Carboxylic Acids / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D037741:Fullerenes / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*
chemical_list: C416673:C60 carboxyfullerene; D002264:Carboxylic Acids; D020099:Coated Materials, Biocompatible; D037741:Fullerenes; D052578:Ionic Liquids; D002244:Carbon; D005949:Glucose Oxidase; D005947:Glucose
keywords: Glucose oxidase; direct electrochemistry; ionic liquid; polyhydroxy-C60