title: An inside-out vein graft filled with platelet-rich plasma for repair of a short sciatic nerve defect in rats.
abstract: Platelet-rich plasma containing various growth factors can promote nerve regeneration. An inside-out vein graft can substitute nerve autograft to repair short nerve defects. It is hypothesized that an inside-out vein graft filled with platelet-rich plasma shows better effects in the repair of short sciatic nerve defects. In this study, an inside-out vein autograft filled with platelet-rich plasma was used to bridge a 10 mm-long sciatic nerve defect in rats. The sciatic nerve function of rats with an inside-out vein autograft filled with platelet-rich plasma was better improved than that of rats with a simple inside-out vein autograft. At 6 and 8 weeks, the sciatic nerve function of rats with an inside-out vein autograft filled with platelet-rich plasma was better than that of rats undergoing nerve autografting. Compared with the sciatic nerve repaired with a simple inside-out vein autograft, the number of myelinated axons was higher, axon diameter and myelin sheath were greater in the sciatic nerve repaired with an inside-out vein autograft filled with platelet-rich plasma and they were similar to those in the sciatic nerve repaired with nerve autograft. These findings suggest that an inside-out vein graft filled with platelet-rich plasma can substitute nerve autograft to repair short sciatic nerve defects.
authors.affiliation: Department of Orthopedic Surgery, Seoul Paik Hospital, Inje University, Seoul, Korea.
authors.forename: Ji Yeong
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Department of Orthopedic Surgery, Korea University, Seoul, Korea.
authors.forename: Woo Joo
authors.initials: WJ
authors.lastname: Jeon
authors.affiliation: Department of Physical Medicine & Rehabilitation, Korea University, Seoul, Korea.
authors.forename: Dong Hwee
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Department of Anatomy, Korea University, Seoul, Korea.
authors.forename: Im Joo
authors.initials: IJ
authors.lastname: Rhyu
authors.affiliation: Department of Orthopedic Surgery, Korea University, Seoul, Korea.
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Biomedical Research Center, Korea Institute of Science and Technology, Seoul, Korea.
authors.forename: Inchan
authors.initials: I
authors.lastname: Youn
authors.affiliation: Department of Orthopedic Surgery, Korea University, Seoul, Korea.
authors.forename: Jong Woong
authors.initials: JW
authors.lastname: Park
keywords: axon diameter; histology; inside-out vein autograft; myelin sheath thickness; myelinated axons; nerve regeneration; neural regeneration; peripheral nerve injury; platelet-rich plasma; sciatic nerve; sciatic nerve index