title: Identification and characterization of chlorogenic acids, chlorogenic acid glycosides and flavonoids from Lonicera henryi L. (Caprifoliaceae) leaves by LC-MSn.
abstract: The chlorogenic acids, chlorogenic acid glycosides and flavonoids of the leaves of Lonicera henryi L. (Caprifoliaceae) were investigated qualitatively by liquid chromatography tandem mass spectrometry. Thirty-one chlorogenic acids and their glycosides were detected and characterized to their regioisomeric level on the basis of their unique fragmentation pattern in the negative ion mode tandem MS spectra. All of them were extracted for the first time from this source and thirteen of them were not reported previously in nature. For the positive identification of chlorogenic acid glycosides by LC-MS(n), multiple reaction monitoring and targeted MS(n) experiments were performed. We have developed an LC-MS(n) method for the systematic identification of chlorogenic acid glycosides and were also able to discriminate between chlorogenic acids and their isobaric glycosides. It was also possible to discriminate between 5-O-(3'-O-caffeoyl glucosyl)quinic acid and 5-O-(4'-O-caffeoyl glucosyl)quinic acid by LC-MS(n). This method can be applied for the rapid and positive identification of chlorogenic acids and their glycosides in plant materials, food and beverages.
authors.affiliation: School of Engineering and Science, Chemistry, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany. Electronic address: r.jaiswal@jacobs-university.de.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Jaiswal
authors.affiliation: School of Engineering and Science, Chemistry, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany.
authors.forename: Heiko
authors.initials: H
authors.lastname: Müller
authors.affiliation: School of Engineering and Science, Chemistry, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Müller
authors.affiliation: School of Engineering and Science, Chemistry, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany.
authors.forename: Mohamed Gamaleldin Elsadig
authors.initials: MG
authors.lastname: Karar
authors.affiliation: School of Engineering and Science, Chemistry, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany. Electronic address: n.kuhnert@jacobs-university.de.
authors.forename: Nikolai
authors.initials: N
authors.lastname: Kuhnert
mesh_terms: D001628:Beverages / Q000032:analysis; D002726:Chlorogenic Acid / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D006027:Glycosides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D031239:Lonicera / Q000737:chemistry*; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D011801:Quinic Acid / Q000737:chemistry / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D005419:Flavonoids; D006027:Glycosides; D011801:Quinic Acid; D002726:Chlorogenic Acid
keywords: Chlorogenic acid glycosides; Chlorogenic acids; Flavonoid glycosides; LC-DAD/ESI-MS(n); Lonicera henryi L.; Polyphenols