title: Chemical composition of bioactive alkaloid extracts from some Narcissus species and varieties and their biological activity.
abstract: Alkaloid extracts of eight Narcissus (Amaryllidaceae) species and varieties were studied with respect to their acetylcholinesterase (HuAChE) and butyrylcholinesterase (HuBuChE) inhibitory activity and alkaloid patterns. Thirty alkaloids were determined by GC/MS, and twenty-five of them identified from their mass spectra, retention times and retention indexes. Promising HuAChE inhibition activity was demonstrated by six Narcissus taxa and HuBuChE inhibition by N. jonquila cv. Double Campernelle and N. nanus cv. Elka with IC50 values of 24.1 +/- 1.9 microg/mL and 25.1 +/- 1.8 microg/mL, respectively. Two alkaloids were isolated in pure form using preparative TLC and identified as the galanthamine type alkaloid narwedine and the lycorine type alkaloid incartine. Both compounds were tested for their biological activity. They were considered inactive in HuAChE/HuBuChE assays, but showed promising prolyl oligopeptidase inhibition activities with IC50 values of 0.95 +/- 0.12 mM and 0.91 ğ 0.09 mM, respectively.
authors.forename: Jana
authors.initials: J
authors.lastname: Havlasová
authors.forename: Marcela
authors.initials: M
authors.lastname: Safratová
authors.forename: Tomás
authors.initials: T
authors.lastname: Siatka
authors.forename: Sárka
authors.initials: S
authors.lastname: Stĕpánková
authors.forename: Zdenĕk
authors.initials: Z
authors.lastname: Novák
authors.forename: Miroslav
authors.initials: M
authors.lastname: Locárek
authors.forename: Lubomír
authors.initials: L
authors.lastname: Opletal
authors.forename: Martina
authors.initials: M
authors.lastname: Hrabinová
authors.forename: Daniel
authors.initials: D
authors.lastname: Jun
authors.forename: Nina
authors.initials: N
authors.lastname: Benesová
authors.forename: Jirí
authors.initials: J
authors.lastname: Kunes
authors.forename: Lucie
authors.initials: L
authors.lastname: Cahlíková
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D031427:Narcissus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D000085822:Prolyl Oligopeptidases; D012697:Serine Endopeptidases / Q000032:analysis
chemical_list: D000470:Alkaloids; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D012697:Serine Endopeptidases; D000085822:Prolyl Oligopeptidases