title: Age-related change in the expression of NKCC1 in the cochlear lateral wall of C57BL/6J mice.
abstract: CONCLUSIONS
  Na(+)-K(+)-2Cl(-) co-transporter isoform 1 (NKCC1) mRNA and protein decrease with increasing age in the cochlear lateral wall of C57BL/6J (C57) mice. The down-regulation of NKCC1 may influence the K(+) transport efficiency and the homeostasis of ion transport cells, and cause the irreversible damage of cochlear cells in old C57 mice. Our results indicate that NKCC1 may play an important role in the pathogenesis of age-related hearing loss (AHL).
  
  
  OBJECTIVE
  The aim of the present study was to investigate the relationship between the functional expression of NKCC1 transporter and the etiology of AHL.
  
  
  METHODS
  C57 mice were used and randomly divided into four groups according to age (4 weeks, 14 weeks, 26 weeks, and 52 weeks). Immunofluorescence technique, quantitative real-time PCR, and western blot were applied to detect the expression of NKCC1 in the cochlear lateral wall of C57 mice at the various ages.
  
  
  RESULTS
  In all four groups, the expression of NKCC1 was observed in the stria vascularis and type II fibrocytes of the spiral ligament. Also, the expression of NKCC1 appeared to decrease with age at both the transcriptional level and the protein level.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan , PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: Hanqi
authors.initials: H
authors.lastname: Chu
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
authors.forename: Liangqiang
authors.initials: L
authors.lastname: Zhou
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Chen
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Zhengong
authors.initials: Z
authors.lastname: Wu
authors.forename: Shaoli
authors.initials: S
authors.lastname: Wang
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Lai
authors.forename: Chunchen
authors.initials: C
authors.lastname: Pan
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Cui
mesh_terms: D000367:Age Factors; D000375:Aging / Q000235:genetics; D000704:Analysis of Variance; D000818:Animals; D003051:Cochlea / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D015536:Down-Regulation; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D034381:Hearing Loss / Q000235:genetics* / Q000503:physiopathology; D015151:Immunoblotting; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000032:analysis; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D064506:Solute Carrier Family 12, Member 2 / Q000235:genetics* / Q000378:metabolism; D013316:Stria Vascularis / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D064506:Solute Carrier Family 12, Member 2
keywords: Age-related hearing loss; Na+-K+-2Cl– co-transporter isoform 1; stria vascularis