title: Light and electron microscopic features of surgically excised left atrial appendage in rheumatic heart disease patients with atrial fibrillation and sinus rhythm.
abstract: BACKGROUND
  There are few studies comparing the pathology of the remodeled substrate in patients of rheumatic heart disease with atrial fibrillation (AF) and normal sinus rhythm (NSR).
  
  
  METHODS
  The study group comprised 30 patients with rheumatic heart disease undergoing mitral valve replacement. Excised left atrial appendages of these patients [17 with persistent AF and 13 NSR (control group)] were subjected to light and electron microscopic examination.
  
  
  RESULTS
  The histopathological findings of the myocardium were characterized by cardiomyocyte hypertrophy (CH), nuclear enlargement (NE), perinuclear clearing (PC), sarcoplasmic vacuolation (SV), fibrosis, and inflammation in the patients with AF and NSR. NE (17/17 vs. 4/13; P=.004), PC (17/17 vs. 4/13; P=.004), SV (17/17 vs. 9/13; P=.06), and fibrosis (15/17 vs. 3/13; P=.001) were all significantly more common in patients with AF. Inflammatory cells were observed in 9/17 patients of AF as compared to 1 in NSR patients (9/17 vs. 1/13; P=.02). CH was common in the patients with AF as compared with those in NSR (17/17 vs. 10/13; P=.103). In AF patients, electron microscopy revealed cardiomyocytes with depletion of the contractile elements (Z-bands), glycogen particle accumulation, and an increase in mitochondria. Cells severely affected by AF showed loss of contractile elements with extensive areas of SV, presence of myelin figures, and mitochondrial aggregates. Majority of AF cases showed extensive fibrosis in the form of collagen bundles in the interstitium.
  
  
  CONCLUSIONS
  The left atrial substrate in AF as compared with NSR, in rheumatic heart disease patients, is associated with significant degenerative remodeling and ongoing inflammation that is associated with extensive fibrosis.
authors.affiliation: Cardiothoracic Center and Cardiac Pathology, All India Institute of Medical Sciences, New Delhi, India 110029.
authors.forename: Shruti
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Cardiothoracic Center and Cardiac Pathology, All India Institute of Medical Sciences, New Delhi, India 110029. Electronic address: drsharmagautam@gmail.com.
authors.forename: Gautam
authors.initials: G
authors.lastname: Sharma
authors.affiliation: Cardiothoracic Center and Cardiac Pathology, All India Institute of Medical Sciences, New Delhi, India 110029.
authors.forename: Milind
authors.initials: M
authors.lastname: Hote
authors.affiliation: Cardiothoracic Center and Cardiac Pathology, All India Institute of Medical Sciences, New Delhi, India 110029.
authors.forename: V
authors.initials: V
authors.lastname: Devagourou
authors.affiliation: Cardiothoracic Center and Cardiac Pathology, All India Institute of Medical Sciences, New Delhi, India 110029.
authors.forename: Vikas
authors.initials: V
authors.lastname: Kesari
authors.affiliation: Cardiothoracic Center and Cardiac Pathology, All India Institute of Medical Sciences, New Delhi, India 110029.
authors.forename: Sudhir
authors.initials: S
authors.lastname: Arava
authors.affiliation: Cardiothoracic Center and Cardiac Pathology, All India Institute of Medical Sciences, New Delhi, India 110029.
authors.forename: Balram
authors.initials: B
authors.lastname: Airan
authors.affiliation: Cardiothoracic Center and Cardiac Pathology, All India Institute of Medical Sciences, New Delhi, India 110029.
authors.forename: Ruma
authors.initials: R
authors.lastname: Ray
mesh_terms: D020517:Atrial Appendage / Q000473:pathology* / Q000601:surgery; D001281:Atrial Fibrillation / Q000150:complications* / Q000473:pathology* / Q000601:surgery; D058385:Cardiac Valve Annuloplasty; D016022:Case-Control Studies; D049108:Cell Enlargement; D006349:Heart Valve Diseases / Q000150:complications / Q000473:pathology / Q000601:surgery; D019918:Heart Valve Prosthesis Implantation; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008943:Mitral Valve / Q000601:surgery; D058386:Mitral Valve Annuloplasty; D032383:Myocytes, Cardiac / Q000473:pathology; D012214:Rheumatic Heart Disease / Q000150:complications* / Q000473:pathology* / Q000601:surgery; D014261:Tricuspid Valve / Q000601:surgery
keywords: Atrial fibrillation; Rheumatic heart disease