title: Filamin A-interacting protein (FILIP) is a region-specific modulator of myosin 2b and controls spine morphology and NMDA receptor accumulation.
abstract: Learning and memory depend on morphological and functional changes to neural spines. Non-muscle myosin 2b regulates actin dynamics downstream of long-term potentiation induction. However, the mechanism by which myosin 2b is regulated in the spine has not been fully elucidated. Here, we show that filamin A-interacting protein (FILIP) is involved in the control of neural spine morphology and is limitedly expressed in the brain. FILIP bound near the ATPase domain of non-muscle myosin heavy chain IIb, an essential component of myosin 2b, and modified the function of myosin 2b by interfering with its actin-binding activity. In addition, FILIP altered the subcellular distribution of myosin 2b in spines. Moreover, subunits of the NMDA receptor were differently distributed in FILIP-expressing neurons, and excitation propagation was altered in FILIP-knockout mice. These results indicate that FILIP is a novel, region-specific modulator of myosin 2b.
authors.affiliation: 1] Division of Cell Biology and Neuroscience, Department of Morphological and Physiological Sciences, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan [2] Research and Education Program for Life Science, University of Fukui, Fukui 910-8507, Japan [3] Department of Anatomy and Neuroscience, Hyogo College of Medicine, Hyogo 663-8501, Japan.
authors.forename: Hideshi
authors.initials: H
authors.lastname: Yagi
authors.affiliation: Division of Cell Biology and Neuroscience, Department of Morphological and Physiological Sciences, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nagano
authors.affiliation: 1] Division of Cell Biology and Neuroscience, Department of Morphological and Physiological Sciences, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan [2] Research and Education Program for Life Science, University of Fukui, Fukui 910-8507, Japan.
authors.forename: Min-Jue
authors.initials: MJ
authors.lastname: Xie
authors.affiliation: 1] Research and Education Program for Life Science, University of Fukui, Fukui 910-8507, Japan [2] Department of Human and Artificial Intelligence Systems, Faculty of Engineering, University of Fukui, Fukui 910-8507, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ikeda
authors.affiliation: 1] Division of Cell Biology and Neuroscience, Department of Morphological and Physiological Sciences, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan [2] Research and Education Program for Life Science, University of Fukui, Fukui 910-8507, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Kuroda
authors.affiliation: 1] Division of Cell Biology and Neuroscience, Department of Morphological and Physiological Sciences, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan [2] Research and Education Program for Life Science, University of Fukui, Fukui 910-8507, Japan.
authors.forename: Munekazu
authors.initials: M
authors.lastname: Komada
authors.affiliation: 1] Division of Cell Biology and Neuroscience, Department of Morphological and Physiological Sciences, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan [2] Research and Education Program for Life Science, University of Fukui, Fukui 910-8507, Japan [3] Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan.
authors.forename: Tokuichi
authors.initials: T
authors.lastname: Iguchi
authors.affiliation: Division of Cell Biology and Neuroscience, Department of Morphological and Physiological Sciences, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan.
authors.forename: Rahman M
authors.initials: RM
authors.lastname: Tariqur
authors.affiliation: 1] Division of Cell Biology and Neuroscience, Department of Morphological and Physiological Sciences, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan [2] Division of Ophthalmology, Department of Sensory and Locomotor Medicine, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan.
authors.forename: Soichi
authors.initials: S
authors.lastname: Morikubo
authors.affiliation: Department of Anatomy and Neuroscience, Hyogo College of Medicine, Hyogo 663-8501, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Noguchi
authors.affiliation: 1] Research and Education Program for Life Science, University of Fukui, Fukui 910-8507, Japan [2] Department of Human and Artificial Intelligence Systems, Faculty of Engineering, University of Fukui, Fukui 910-8507, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Murase
authors.affiliation: Department of Experimental Genome Research, Genome Information Research Center, Osaka University, Osaka 565-0871, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Okabe
authors.affiliation: 1] Division of Cell Biology and Neuroscience, Department of Morphological and Physiological Sciences, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan [2] Research and Education Program for Life Science, University of Fukui, Fukui 910-8507, Japan [3] Research Center for Child Mental Development, University of Fukui, Fukui 910-1193, Japan [4] United Graduate School of Child Development, Osaka University, Kanazawa University, Hamamatsu University School of Medicine, Chiba University and University of Fukui, Osaka 565-0871, Japan [5] Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Sato
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000502:physiology*; D002478:Cells, Cultured; D049229:Dendritic Spines / Q000737:chemistry* / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D047468:Immunoprecipitation; D017774:Long-Term Potentiation; D051379:Mice; D008813:Mice, Inbred ICR; D018345:Mice, Knockout; D018995:Myosin Heavy Chains / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D024501:Nonmuscle Myosin Type IIB / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*
chemical_list: D000199:Actins; D002352:Carrier Proteins; C500538:FILIP protein, mouse; D016194:Receptors, N-Methyl-D-Aspartate; D024501:Nonmuscle Myosin Type IIB; C103815:nonmuscle myosin type IIB heavy chain; D018995:Myosin Heavy Chains