title: Lactobacillus gorillae sp. nov., isolated from the faeces of captive and wild western lowland gorillas (Gorilla gorilla gorilla).
abstract: Four strains of Gram-staining-positive, anaerobic rods were isolated from the faeces of western lowland gorillas (Gorilla gorilla gorilla). Three strains, KZ01(T), KZ02 and KZ03, were isolated at the Kyoto City Zoo, Japan, and one strain, GG02, was isolated in the Moukalaba-Doudou National Park, Gabon. These strains were investigated taxonomically. These strains belonged to the Lactobacillus reuteri phylogenetic group according to phylogenetic analysis based on 16S rRNA gene sequences and specific phenotypic characteristics. Phylogenetic analysis of their 16S rRNA gene sequences revealed that strains KZ01(T), KZ02, KZ03 and GG02 formed a single monophyletic cluster and had a distinct line of descent. Based on sequence similarity of the 16S rRNA gene, Lactobacillus fermentum JCM 1173(T) (96.6 %) was the closest neighbour to these novel strains, although it was clear that these strains belonged to a different species. Partial pheS sequences also supported these relationships. DNA-DNA relatedness between strain KZ01(T) and L. fermentum JCM 1173(T) was less than 22 % and the DNA G+C content of strain KZ01(T) was 50.7 mol%. The cell-wall peptidoglycan type was A4β (l-Orn-d-Asp) and the major fatty acids were C16 : 0, C18 : 1ω9c and C19 : 1 cyclo 9,10. Therefore, based on phylogenetic, phenotypic and physiological evidence, these strains represent a novel species of the genus Lactobacillus, for which the name Lactobacillus gorillae sp. nov. is proposed. The type strain is KZ01(T) ( = JCM 19575(T) = DSM 28356(T)).
authors.affiliation: Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Shimogamo, Kyoto 606-8522, Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Tsuchida
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Kitahara
authors.affiliation: Research Institute of Tropical Ecology, Libreville, Bp 13354, Gabon.
authors.forename: Pierre Philippe Mbehang
authors.initials: PPM
authors.lastname: Nguema
authors.affiliation: Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Shimogamo, Kyoto 606-8522, Japan.
authors.forename: Saeko
authors.initials: S
authors.lastname: Norimitsu
authors.affiliation: Graduate School of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
authors.forename: Shiho
authors.initials: S
authors.lastname: Fujita
authors.affiliation: Graduate School of Science, Kyoto University, Kitashirakawa, Kyoto 606-8502, Japan.
authors.forename: Juichi
authors.initials: J
authors.lastname: Yamagiwa
authors.affiliation: Research Institute of Tropical Ecology, Libreville, Bp 13354, Gabon.
authors.forename: Alfred
authors.initials: A
authors.lastname: Ngomanda
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Moriya
authors.initials: M
authors.lastname: Ohkuma
authors.affiliation: Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Shimogamo, Kyoto 606-8522, Japan.
authors.forename: Kazunari
authors.initials: K
authors.lastname: Ushida
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005243:Feces / Q000382:microbiology; D005260:Female; D005681:Gabon; D006071:Gorilla gorilla / Q000382:microbiology*; D007564:Japan; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S