title: Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism.
abstract: DNJ, an inhibitor of α-glucosidase, is used to suppress the elevation of postprandial hyperglycemia. In this study, we focus on screening an appropriate microorganism for performing fermentation to improve DNJ content in mulberry leaf. Results showed that Ganoderma lucidum was selected from 8 species and shown to be the most effective in improvement of DNJ production from mulberry leaves through fermentation. Based on single factor and three factor influence level tests by following the Plackett-Burman design, the optimum extraction yield was analyzed by response surface methodology (RSM). The extracted DNJ was determined by reverse-phase high performance liquid chromatograph equipped with fluorescence detector (HPLC-FD). The results of RSM showed that the optimal condition for mulberry fermentation was defined as pH 6.97, potassium nitrate content 0.81% and inoculums volume 2 mL. The extraction efficiency reached to 0.548% in maximum which is 2.74 fold of those in mulberry leaf.
authors.affiliation: Jiangsu University of Science and Technology Zhenjiang China Jiangsu University of Science and Technology, Zhenjiang, China.
authors.forename: Yun-Gang
authors.initials: YG
authors.lastname: Jiang
authors.affiliation: Jiangsu University of Science and Technology Zhenjiang China Jiangsu University of Science and Technology, Zhenjiang, China. ; Department of Biological and Environmental Engineering Hefei University Hefei China Department of Biological and Environmental Engineering, Hefei University, Hefei, China.
authors.forename: Chu-Yan
authors.initials: CY
authors.lastname: Wang
authors.affiliation: Jiangsu University of Science and Technology Zhenjiang China Jiangsu University of Science and Technology, Zhenjiang, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Jin
authors.affiliation: Jiangsu University of Science and Technology Zhenjiang China Jiangsu University of Science and Technology, Zhenjiang, China. ; Sericultural Research Institute Chinese Academy of Agricultural Sciences Zhenjiang China Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, China.
authors.forename: Jun-Qiang
authors.initials: JQ
authors.lastname: Jia
authors.affiliation: Jiangsu University of Science and Technology Zhenjiang China Jiangsu University of Science and Technology, Zhenjiang, China. ; Sericultural Research Institute Chinese Academy of Agricultural Sciences Zhenjiang China Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, China.
authors.forename: Xijie
authors.initials: X
authors.lastname: Guo
authors.affiliation: Jiangsu University of Science and Technology Zhenjiang China Jiangsu University of Science and Technology, Zhenjiang, China. ; Sericultural Research Institute Chinese Academy of Agricultural Sciences Zhenjiang China Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, China.
authors.forename: Guo-Zheng
authors.initials: GZ
authors.lastname: Zhang
authors.affiliation: Jiangsu University of Science and Technology Zhenjiang China Jiangsu University of Science and Technology, Zhenjiang, China. ; Sericultural Research Institute Chinese Academy of Agricultural Sciences Zhenjiang China Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, China.
authors.forename: Zhong-Zheng
authors.initials: ZZ
authors.lastname: Gui
mesh_terms: D017485:1-Deoxynojirimycin / Q000302:isolation & purification* / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D031625:Morus / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D020940:Reishi / Q000254:growth & development / Q000378:metabolism*; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D003470:Culture Media; D004791:Enzyme Inhibitors; D017485:1-Deoxynojirimycin
keywords: DNJ Production; fermentation; microorganisms; mulberry