title: Use of orange peel extract for mixotrophic cultivation of Chlorella vulgaris: increased production of biomass and FAMEs.
abstract: Mass cultivation of microalgae is necessary to achieve economically feasible production of microalgal biodiesel, but the high cost of nutrients is a major limitation. In this study, orange peel extract (OPE) was used as an inorganic and organic nutrient source for the cultivation of Chlorella vulgaris OW-01. Chemical composition analysis of the OPE indicated that it contains sufficient nutrients for mixotrophic cultivation of C. vulgaris OW-01. Analysis of biomass and FAME production showed that microalgae grown in OPE medium produced 3.4-times more biomass and 4.5-times more fatty acid methyl esters (FAMEs) than cells cultured in glucose-supplemented BG 11 medium (BG-G). These results suggest that growth of microalgae in an OPE-supplemented medium increases lipid production and that OPE has potential for use in the mass cultivation of microalgae.
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Won-Kun
authors.initials: WK
authors.lastname: Park
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Myounghoon
authors.initials: M
authors.lastname: Moon
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Min-Su
authors.initials: MS
authors.lastname: Kwak
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Seungjib
authors.initials: S
authors.lastname: Jeon
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Gang-Guk
authors.initials: GG
authors.lastname: Choi
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea; Advanced Biomass R&D Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Yang
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. Electronic address: bongsoolee@kaist.ac.kr.
authors.forename: Bongsoo
authors.initials: B
authors.lastname: Lee
mesh_terms: D056804:Biofuels; D018533:Biomass; D018929:Cell Culture Techniques / Q000379:methods; D048408:Chlorella vulgaris / Q000254:growth & development* / Q000378:metabolism; D032084:Citrus sinensis / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry*; D005227:Fatty Acids / Q000096:biosynthesis*; D005638:Fruit / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000378:metabolism*
chemical_list: D056804:Biofuels; D003470:Culture Media; D005227:Fatty Acids; D010936:Plant Extracts
keywords: Biomass production; Chlorella vulgaris; Cost effective cultivation; FAME production; Orange peel extract