title: Acinetobacter gandensis sp. nov. isolated from horse and cattle.
abstract: We previously reported the presence of an OXA-23 carbapenemase in an undescribed species of the genus Acinetobacter isolated from horse dung at the Faculty of Veterinary Medicine, Ghent University, Belgium. Here we include six strains to corroborate the delineation of this taxon by phenotypic characterization, DNA-DNA hybridization, 16S rRNA gene and rpoB sequence analysis, % G+C determination, MALDI-TOF MS and fatty acid analysis. The nearly complete 16S rRNA gene sequence of strain UG 60467(T) showed the highest similarities with those of the type strains of Acinetobacter bouvetii (98.4 %), Acinetobacter haemolyticus (97.7 %), and Acinetobacter schindleri (97.2 %). The partial rpoB sequence of strain UG 60467(T) showed the highest similarities with 'Acinetobacter bohemicus' ANC 3994 (88.6 %), A. bouvetii NIPH 2281 (88.6 %) and A. schindleri CIP 107287T (87.3 %). Whole-cell MALDI-TOF MS analyses supported the distinctness of the group at the protein level. The predominant fatty acids of strain UG 60467(T) were C12 : 0 3-OH, C12 : 0, C16 : 0, C18 : 1ω9c and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). Strains UG 60467(T) and UG 60716 showed a DNA-DNA relatedness of 84 % with each other and a DNA-DNA relatedness with A. schindleri LMG 19576(T) of 17 % and 20 %, respectively. The DNA G+C content of strain UG 60467(T) was 39.6 mol%. The name Acinetobacter gandensis sp. nov. is proposed for the novel taxon. The type strain is UG 60467(T) ( = ANC 4275(T) = LMG 27960(T) = DSM 28097(T)).
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan133, 9820 Merelbeke, Belgium.
authors.forename: Annemieke
authors.initials: A
authors.lastname: Smet
authors.affiliation: Laboratory Bacteriology Research (LBR), Department of Clinical Chemistry, Microbiology & Immunology, Ghent University Hospital, De Pintelaan 185, 9000 Gent, Belgium.
authors.forename: Piet
authors.initials: P
authors.lastname: Cools
authors.affiliation: Laboratory of Bacterial Genetics, National Institute of Public Health, Srobarova 48, 100 42 Prague, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Krizova
authors.affiliation: Laboratory of Bacterial Genetics, National Institute of Public Health, Srobarova 48, 100 42 Prague, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Maixnerova
authors.affiliation: Research Group Proteomics, Central European Institute of Technology and National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Sedo
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan133, 9820 Merelbeke, Belgium.
authors.forename: Freddy
authors.initials: F
authors.lastname: Haesebrouck
authors.affiliation: Laboratoire de Bactériologie, Institut de Biologie en Santé - PBU, CHU, 4 rue Larrey, 49933 Angers cedex, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Kempf
authors.affiliation: Laboratory of Bacterial Genetics, National Institute of Public Health, Srobarova 48, 100 42 Prague, Czech Republic.
authors.forename: Alexandr
authors.initials: A
authors.lastname: Nemec
authors.affiliation: Laboratory Bacteriology Research (LBR), Department of Clinical Chemistry, Microbiology & Immunology, Ghent University Hospital, De Pintelaan 185, 9000 Gent, Belgium.
authors.forename: Mario
authors.initials: M
authors.lastname: Vaneechoutte
mesh_terms: D000150:Acinetobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001530:Belgium; D002417:Cattle / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005243:Feces / Q000382:microbiology; D005798:Genes, Bacterial; D006736:Horses / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S