title: Development of novel simple sequence repeat markers in bitter gourd (Momordica charantia L.) through enriched genomic libraries and their utilization in analysis of genetic diversity and cross-species transferability.
abstract: Microsatellite or simple sequence repeat (SSR) markers are the preferred markers for genetic analyses of crop plants. The availability of a limited number of such markers in bitter gourd (Momordica charantia L.) necessitates the development and characterization of more SSR markers. These were developed from genomic libraries enriched for three dinucleotide, five trinucleotide, and two tetranucleotide core repeat motifs. Employing the strategy of polymerase chain reaction-based screening, the number of clones to be sequenced was reduced by 81 % and 93.7 % of the sequenced clones contained in microsatellite repeats. Unique primer-pairs were designed for 160 microsatellite loci, and amplicons of expected length were obtained for 151 loci (94.4 %). Evaluation of diversity in 54 bitter gourd accessions at 51 loci indicated that 20 % of the loci were polymorphic with the polymorphic information content values ranging from 0.13 to 0.77. Fifteen Indian varieties were clearly distinguished indicative of the usefulness of the developed markers. Markers at 40 loci (78.4 %) were transferable to six species, viz. Momordica cymbalaria, Momordica subangulata subsp. renigera, Momordica balsamina, Momordica dioca, Momordica cochinchinesis, and Momordica sahyadrica. The microsatellite markers reported will be useful in various genetic and molecular genetic studies in bitter gourd, a cucurbit of immense nutritive, medicinal, and economic importance.
authors.affiliation: Division of Genomic Resources, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, 110012, India.
authors.forename: Swati
authors.initials: S
authors.lastname: Saxena
authors.forename: Archana
authors.initials: A
authors.lastname: Singh
authors.forename: Sunil
authors.initials: S
authors.lastname: Archak
authors.forename: Tushar K
authors.initials: TK
authors.lastname: Behera
authors.forename: Joseph K
authors.initials: JK
authors.lastname: John
authors.forename: Sudhir U
authors.initials: SU
authors.lastname: Meshram
authors.forename: Ambika B
authors.initials: AB
authors.lastname: Gaikwad
mesh_terms: D000483:Alleles; D005819:Genetic Markers; D014644:Genetic Variation; D018745:Genome, Plant; D015698:Genomic Library; D018895:Microsatellite Repeats / Q000235:genetics*; D029777:Momordica charantia / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D005819:Genetic Markers