title: Diversified expression of aryl hydrocarbon receptor dependent genes in human laryngeal squamous cell carcinoma cell lines treated with β-naphthoflavone.
abstract: The aryl hydrocarbon receptor (AhR) mediates a variety of biological responses to ubiquitous environmental pollutants. In this study the effect of administration of β-naphthoflavone (BNF), potent AhR ligand, on the expression of AhR, AhRR, CYP1A1, CYP1A2, CYP1B1, NQO1, GSTA1, ALDH3A1 and UGT1A genes encoding the enzymes controlled by AhR were examined in thirteen laryngeal tumor cell lines and in HepaRG cell line. The analyzed cell lines were derived from patients with squamous laryngeal cancer, with history of cigarette smoking and without signs of human papillomavirus types 16 and 18 infection in investigated cells. Quantitative real-time RT-PCR analysis revealed huge interindividual differences in expression of genes from AhR regulatory network. Our results strongly suggest predominant effect of DNA methylation on induction of CYP1A1 expression by AhR ligands as well. Our results indicate that differentiated HepaRG cell line appeared to be very good substitute for human liver in studies on xenobiotic metabolism by AhR regulated enzymes.
authors.affiliation: Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland. Electronic address: brauzdam@man.poznan.pl.
authors.forename: Damian
authors.initials: D
authors.lastname: Brauze
authors.affiliation: Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Fijalkiewicz
authors.affiliation: Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Szaumkessel
authors.affiliation: Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kiwerska
authors.affiliation: Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland.
authors.forename: Kinga
authors.initials: K
authors.lastname: Bednarek
authors.affiliation: Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland; Department of Medical Genetics, Medical University of Warsaw, Pawinskiego 3c, 02-106 Warsaw, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Rydzanicz
authors.affiliation: Institute of Human Genetics, University Hospital Schleswig-Holstein, Campus Kiel/Christian-Albrechts-University Kiel, 24105 Kiel, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Richter
authors.affiliation: Department of Otorhinolaryngology - Head and Neck Surgery and Department of Medical Biochemistry, Turku University Central Hospital and Turku University, P.O. Box 52, FIN-20521 Turku, Finland.
authors.forename: Reidar
authors.initials: R
authors.lastname: Grenman
authors.affiliation: Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Jarmuz-Szymczak
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D002274:Carcinogens, Environmental / Q000633:toxicity*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis / Q000235:genetics; D019175:DNA Methylation / Q000187:drug effects; D004790:Enzyme Induction; D044127:Epigenesis, Genetic / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D020084:Long Interspersed Nucleotide Elements / Q000187:drug effects; D060888:Real-Time Polymerase Chain Reaction; D018336:Receptors, Aryl Hydrocarbon / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019324:beta-Naphthoflavone / Q000633:toxicity*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D002274:Carcinogens, Environmental; D019363:Cytochrome P-450 CYP1A1; D008024:Ligands; D018336:Receptors, Aryl Hydrocarbon; D019324:beta-Naphthoflavone; C111235:AHR protein, human; C569463:CYP1A1 protein, human
keywords: AhR; AhRR; CYP1A1; CYP1A2; CYP1B1; GSTA1; NQO1; SCC cell lines; UGT1A1; β-naphthoflavone