title: Molecular cloning, sequence analysis and tissue-specific expression of Akirin2 gene in Tianfu goat.
abstract: The Akirin2 gene is a nuclear factor and is considered as a potential functional candidate gene for meat quality. To better understand the structures and functions of Akirin2 gene, the cDNA of the Tianfu goat Akirin2 gene was cloned. Sequence analysis showed that the Tianfu goat Akirin2 cDNA full coding sequence (CDS) contains 579bp nucleotides that encode 192 amino acids. A phylogenic tree of the Akirin2 protein sequence from the Tianfu goat and other species revealed that the Tianfu goat Akirin2 was closely related with cattle and sheep Akirin2. RT-qPCR analysis showed that Akirin2 was expressed in the myocardium, liver, spleen, lung, kidney, leg muscle, abdominal muscle and the longissimus dorsi muscle. Especially, high expression levels of Akirin2 were detected in the spleen, lung, and kidney whereas lower expression levels were seen in the liver, myocardium, leg muscle, abdominal muscle and longissimus dorsi muscle. Temporal mRNA expression showed that Akirin2 expression levels in the longissimus dorsi muscle, first increased then decreased from day 1 to month 12. Western blotting results showed that the Akirin2 protein was only detected in the lung and three skeletal muscle tissues.
authors.affiliation: Institution of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, 46# Xinkang Road, Yucity District, Yaan 625014, Sichuan Province, China.
authors.forename: Jisi
authors.initials: J
authors.lastname: Ma
authors.affiliation: Institution of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, 46# Xinkang Road, Yucity District, Yaan 625014, Sichuan Province, China. Electronic address: goatsxgy@263.net.
authors.forename: Gangyi
authors.initials: G
authors.lastname: Xu
authors.affiliation: Institution of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, 46# Xinkang Road, Yucity District, Yaan 625014, Sichuan Province, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wan
authors.affiliation: Institution of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, 46# Xinkang Road, Yucity District, Yaan 625014, Sichuan Province, China.
authors.forename: Nianlu
authors.initials: N
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006041:Goats / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012756:Sheep
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D012097:Repressor Proteins
keywords: Akirin2 gene; Complete CDS; Expression analysis; Sequence analysis; Tianfu goat