title: Microfluidic whole genome amplification device for single cell sequencing.
abstract: We developed a microfluidic device to perform multiplex single-cell whole-genome amplification (WGA) using multiple annealing and looping-based amplification cycles (MALBAC). This device, made of polydimethylsiloxane (PDMS), allows us to monitor the whole process of cell loading and single-cell WGA for sequencing. We show that the genome coverage of MALBAC amplifications is reproducible between chambers on a single chip and between different chips, which enables data normalization using standard samples to accurately identify copy number variations (CNVs). This device provides an easy-to-operate approach to perform single cell sequencing library preparation with minimum hands-on time. It reduces the requirement of manual expertise as well as the risk of contamination, which is essential in future applications especially the medical diagnosis.
authors.affiliation: Biodynamic Optical Imaging Center (BIOPIC) and College of Engineering, Peking University , Beijing 100871, China.
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Yu
authors.forename: Sijia
authors.initials: S
authors.lastname: Lu
authors.forename: Yanyi
authors.initials: Y
authors.lastname: Huang
mesh_terms: D001482:Base Composition; D056915:DNA Copy Number Variations; D004129:Dimethylpolysiloxanes; D015723:Gene Library; D015894:Genome, Human; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D059010:Single-Cell Analysis / Q000295:instrumentation* / Q000379:methods
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon