title: Expression of Treg subsets on intestinal T cell immunity and endotoxin translocation in porcine sepsis after severe burns.
abstract: In the present study, the changes of the regulatory T cells (Treg) expression, endotoxin translocation, and the relationships in intestinal lymph nodes were investigated in porcine sepsis induced by severe burns. Flow cytometry, western blot, and Tachypleus amebocyte lysate were applied to study after the burn injury model was built. We found that the upregulated Treg expression was negatively related to the CD3(+)CD4(+)/CD3(+)CD8(+) ratio (r = -0.832, P < 0.05) after burn injury-induced sepsis. While Treg expression and portal venous plasma endotoxin translocation levels were positively correlated (r = 0.876, P < 0.05) when compared with the control group. Moreover, we detected a transforming of T cell subsets from T helper 1 cells to T helper 2 cells. Therefore, intestinal Treg cells expression exerts immunosuppressive effects on other intestinal T lymphocytes and was closely related to endotoxin translocation in porcine sepsis after severe burns injuries. Above all, the intestinal Treg cells may play an important role in the intestinal immune barrier system after severe burns injuries.
authors.affiliation: Department of Burns, First Affiliated Hospital of Henan University of Science and Technology, Luoyang, China, drhongxuzu@163.com.
authors.forename: HongXu
authors.initials: H
authors.lastname: Zu
authors.forename: QingHua
authors.initials: Q
authors.lastname: Li
authors.forename: PeiXin
authors.initials: P
authors.lastname: Huang
mesh_terms: D000818:Animals; D017209:Apoptosis; D002056:Burns / Q000150:complications / Q000473:pathology*; D017252:CD3 Complex / Q000378:metabolism; D015704:CD4 Antigens / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D015536:Down-Regulation; D004731:Endotoxins / Q000097:blood / Q000378:metabolism*; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D008297:Male; D018805:Sepsis / Q000209:etiology / Q000276:immunology*; D012720:Severity of Illness Index; D013552:Swine; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism*; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism
chemical_list: D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D016207:Cytokines; D004731:Endotoxins; D053645:Interleukin-2 Receptor alpha Subunit