title: Development of an immunochromatographic strip test for rapid detection of ciprofloxacin in milk samples.
abstract: A rapid, simple, and sensitive immunochromatographic test strip has been developed for testing residues of ciprofloxacin (CIP). A specific and sensitive monoclonal antibody (mAb) for CIP was generated by immunizing BALB/c mice with well-characterized CIP-Keyhole limpet haemocyanin. Under the optimized conditions, the cut-off limits of test strips for CIP were found to be 5 ng/mL in phosphate-buffered saline and 2.5 ng/mL in milk samples. Each test can be evaluated within 3 min. The cross-reactivities of the CIP test strip to enrofloxacin (ENR), norfloxacin (NOR), nadifloxacin (NDF), danofloxacin (DANO), pefloxacin (PEX), lomefloxacin (LOME), enoxacin (ENO), and sarafloxacin (SAR) were 71.4%, 71.4%, 66%, 50%, 33%, 20%, 12.5%, and 6.25%, respectively. The data indicate that the method is sensitive, specific, and has the advantages of simplicity and speed, therefore, this test strip is a useful screening method for the detection of CIP residues in milk samples.
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. raxray@gmail.com.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Liu
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. raxray@gmail.com.
authors.forename: Liju
authors.initials: L
authors.lastname: Luo
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. steven_090287@yahoo.co.id.
authors.forename: Steven
authors.initials: S
authors.lastname: Suryoprabowo
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. pengjuan2016@163.com.
authors.forename: Juan
authors.initials: J
authors.lastname: Peng
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. kuangh@jiangnan.edu.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. xcl@jiangnan.edu.cn.
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002417:Cattle; D002846:Chromatography, Affinity / Q000295:instrumentation*; D002939:Ciprofloxacin / Q000032:analysis* / Q000276:immunology; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D011934:Reagent Strips
chemical_list: D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D011934:Reagent Strips; D002939:Ciprofloxacin