title: Comparison of toxicity between saxitoxin and decarbamoyl saxitoxin in the mouse bioassay for paralytic shellfish poisoning toxins.
abstract: The mouse bioassay (MBA) for paralytic shellfish poisoning (PSP) toxins has been used in the AOAC Official Method and the official Japanese method. In the AOAC Official Method, the saxitoxin (STX) standard provided by the U.S. Food and Drug Administration (FDA) is used, but no standard is used in the official Japanese method. The objective of this study was to compare the toxicity of decarbamoyl STX (dcSTX), one of the derivatives of STX and a candidate standard for the MBA for PSP toxins in Japan, to that of FDA STX in the MBA platform. In this study, the toxicity of dcSTX was 918.0 ± 44.9 mouse units/µmol, and the relative toxicity ratio of dcSTX to FDA STX based on moles was 0.478.
authors.affiliation: Division of Biomedical Food Research, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Hodaka
authors.initials: H
authors.lastname: Suzuki
authors.forename: Kenji
authors.initials: K
authors.lastname: Machii
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods / Q000592:standards*; D007564:Japan; D051379:Mice; D012015:Reference Standards; D012530:Saxitoxin / Q000031:analogs & derivatives* / Q000633:toxicity*; D012758:Shellfish / Q000032:analysis*; D057096:Shellfish Poisoning / Q000175:diagnosis*; D013047:Specific Pathogen-Free Organisms; D014020:Tissue Extracts / Q000633:toxicity*
chemical_list: D014020:Tissue Extracts; D012530:Saxitoxin; C034903:decarbamylsaxitoxin