title: The structure of O-polysaccharide isolated from Cronobacter universalis NCTC 9529T.
abstract: The O-polysaccharide (OPS) was isolated from Cronobacter universalis NCTC 9529(T), a new species in the genus Cronobacter, which was created by the reclassification of the species Enterobacter sakazakii. Purified polysaccharide was analyzed by NMR spectroscopy ((1)H, COSY, TOCSY, ROESY, HSQC, and HSQC-TOCSY) and chemical methods. The monosaccharide derivatives were analyzed by gas chromatography and gas chromatography-mass spectrometry. These experiments enabled the type and number of monosaccharides in the repeating unit of OPS, their positions of linkages, and absolute configuration to be determined. Together the chemical analysis established a structure of the OPS of C. universalis NCTC 9529(T). [structure: see text]. OPS isolated from C. universalis was structurally characterized for the first time.
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland.
authors.forename: Kinga
authors.initials: K
authors.lastname: Marszewska
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Czerwicka
authors.affiliation: School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, United Kingdom.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Forsythe
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Sałdak
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Szulta
authors.affiliation: Department of Medical Microbiology, Medical University of Gdansk, Do Studzienki 38, 80-227 Gdansk, Poland.
authors.forename: Halina
authors.initials: H
authors.lastname: Dziadziuszko
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Ossowska
authors.affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland. Electronic address: zbigniew.kaczynski@ug.edu.pl.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Kaczyński
mesh_terms: D002240:Carbohydrate Sequence; D059126:Cronobacter / Q000737:chemistry*; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D019081:O Antigens
keywords: Cronobacter sakazakii; Cronobacter universalis; NMR; O-polysaccharide; Structure