title: Three months of high-fructose feeding fails to induce excessive weight gain or leptin resistance in mice.
abstract: High-fructose diets have been implicated in obesity via impairment of leptin signaling in humans and rodents. We investigated whether fructose-induced leptin resistance in mice could be used to study the metabolic consequences of fructose consumption in humans, particularly in children and adolescents. Male C57Bl/6 mice were weaned to a randomly assigned diet: high fructose, high sucrose, high fat, or control (sugar-free, low-fat). Mice were maintained on their diets for at least 14 weeks. While fructose-fed mice regularly consumed more kcal and expended more energy, there was no difference in body weight compared to control by the end of the study. Additionally, after 14 weeks, both fructose-fed and control mice displayed similar leptin sensitivity. Fructose-feeding also did not change circulating glucose, triglycerides, or free fatty acids. Though fructose has been linked to obesity in several animal models, our data fail to support a role for fructose intake through food lasting 3 months in altering of body weight and leptin signaling in mice. The lack of impact of fructose in the food of growing mice on either body weight or leptin sensitivity over this time frame was surprising, and important information for researchers interested in fructose and body weight regulation.
authors.affiliation: Department of Biology, Williams College, Williamstown, Massachusetts, United States of America.
authors.forename: Erik J
authors.initials: EJ
authors.lastname: Tillman
authors.affiliation: Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, Iowa, United States of America.
authors.forename: Donald A
authors.initials: DA
authors.lastname: Morgan
authors.affiliation: Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, Iowa, United States of America.
authors.forename: Kamal
authors.initials: K
authors.lastname: Rahmouni
authors.affiliation: Department of Biology, Williams College, Williamstown, Massachusetts, United States of America.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Swoap
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001481:Basal Metabolism; D004032:Diet; D059305:Diet, High-Fat / Q000009:adverse effects; D005260:Female; D066248:High Fructose Corn Syrup / Q000008:administration & dosage*; D020738:Leptin / Q000502:physiology*; D008297:Male; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000209:etiology / Q000378:metabolism; D015430:Weight Gain
chemical_list: D066248:High Fructose Corn Syrup; D020738:Leptin