title: A dual amplification strategy for DNA detection combining bio-barcode assay and metal-enhanced fluorescence modality.
abstract: Silver-enhanced fluorescence was coupled with a bio-barcode assay to facilitate a dual amplification assay to demonstrate a non-enzymatic approach for simple and sensitive detection of DNA. In the assay design, magnetic nanoparticles seeded with silver nanoparticles were modified with the capture DNA, and silver nanoparticles were modified with the binding of ssDNA and the fluorescently labeled barcode dsDNA. Upon introduction of the target DNA, a sandwich structure was formed because of the hybridization reaction. By simple magnetic separation, silver-enhanced fluorescence of barcode DNAs could be readily measured without the need of a further step to liberate barcode DNAs from silver nanoparticles, endowing the method with simplicity and high sensitivity with a detection limit of 1 pM.
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China. lina@pku.edu.cn.
authors.forename: Zhenpeng
authors.initials: Z
authors.lastname: Zhou
authors.forename: Tian
authors.initials: T
authors.lastname: Li
authors.forename: Hongduan
authors.initials: H
authors.lastname: Huang
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Chengzhi
authors.initials: C
authors.lastname: Huang
authors.forename: Na
authors.initials: N
authors.lastname: Li
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D005453:Fluorescence; D053768:Metal Nanoparticles / Q000737:chemistry; D012834:Silver / Q000737:chemistry*
chemical_list: D012834:Silver; D004247:DNA