title: The complete mitochondrial genome of Holland's spinibarbel Spinibarbus hollandi Oshima (Cypriniformes, Cyprinidae).
abstract: In this study, the complete mitogenome sequence of cyprinid fish, Holland's spinibarbel Spinibarbus hollandi Oshima (1919) (Cypriniformes, Cyprinidae, Barbinae) has been amplified and sequenced by employing long polymerase chain reaction method. The mitogenome, consisting of 16,523 base pairs (bp), had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes, and a non-coding control region (CR). CR of 867 bp length is located between tRNA(Pro) and tRNA(Phe). The overall base composition of S. hollandi is 31.87% for A, 26.56% for C, 25.89% for T, and 15.68% for G, with a slight AT bias of 57.76%. The complete mitogenome may provide rather essential and important DNA molecular data for further phylogenetic analysis for not only congeneric species but also higher different taxa of Cyprinid fishes.
authors.affiliation: a Institute of Marine Biology, National Taiwan Ocean University , Keelung , Taiwan , ROC .
authors.forename: I-Shiung
authors.initials: IS
authors.lastname: Chen
authors.affiliation: a Institute of Marine Biology, National Taiwan Ocean University , Keelung , Taiwan , ROC .
authors.forename: Jia-Chin
authors.initials: JC
authors.lastname: Bai
authors.affiliation: b National Museum of Marine Biology & Aquarium , Checheng , Pingtung , Taiwan , ROC , and.
authors.forename: Chiao-Chuan
authors.initials: CC
authors.lastname: Han
authors.affiliation: c Department of Sports , Health and Leisure, Cheng Shiu University , Kaohsiung , Taiwan , ROC.
authors.forename: Lee-Shing
authors.initials: LS
authors.lastname: Fang
mesh_terms: D000818:Animals; D020029:Base Pairing / Q000235:genetics; D001483:Base Sequence; D003530:Cyprinidae / Q000235:genetics*; D050259:Genes, Mitochondrial; D054629:Genome, Mitochondrial; D009426:Netherlands; D012343:RNA, Transfer / Q000235:genetics
chemical_list: D012343:RNA, Transfer
keywords: Barbinae; Cyprinidae; Spinibarbus hollandi; Taiwan; mitogenome