title: Fluorescence in situ hybridization for detection of small RNAs on frozen tissue sections.
abstract: MicroRNAs are an abundant class of small noncoding RNAs that regulate and fine-tune the expression of protein-coding genes. Each microRNA regulates around 100 genes, and they are mostly conserved and abundant within the multicellular organisms. Even though microRNAs have a role in many biological processes and diseases, the function of each single microRNA is still yet to be explored in all tissues and cells they are present. Therefore, an efficient in situ hybridization method, combining locked nucleic acid technology and tyramide signal amplification system, has been developed and presented for detection of microRNAs in frozen section at a cellular resolution and with high sensitivity.
authors.affiliation: Department of Cellular and Molecular Medicine, Health and Medical Sciences Faculty, University of Copenhagen, 2200, Copenhagen N, Denmark, asli@sund.ku.dk.
authors.forename: Asli
authors.initials: A
authors.lastname: Silahtaroglu
mesh_terms: D000818:Animals; D005629:Frozen Sections / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D051379:Mice; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D009841:Oligonucleotides / Q000032:analysis / Q000235:genetics
chemical_list: D035683:MicroRNAs; D009841:Oligonucleotides; C477371:locked nucleic acid