title: Radiographic contrast media alterate the localization of actin/band4.9 in the membrane cytoskeleton of human erythrocytes.
abstract: Different radiographic contrast media (RCM) were shown to induce morphological changes of blood cells (e.g. erythrocytes or thrombocytes) and endothelial cells. The echinocytic shape change of erythrocytes, particularly, affords alterations of the membrane cytoskeleton. The cytoskeleton plays a crucial role for the shape and deformability of the red blood cell. Disruption of the interaction between components of the red blood cell membrane cytoskeleton may cause a loss of structural and functional integrity of the membrane. In this study band4.9 and actin as components of the cytoskeletal junctional complex were examined in human erythrocytes after suspension in autologous plasma or in plasma RCM mixtures (30% v/v Iodixanol-320 or Iopromide-370) followed by a successive double staining with TRITC-/FITC-coupled monoclonal antibodies. After adding Iopromide-370 to the plasma in practically none of the cells the rounded conformation of the membrane cytoskeleton - as it appeared in cells suspended in autologous plasma - was found. In addition, Iopromide-370 induced thin lines and coarse knob-like structures of band4.9 at the cell periphery while most cell centers were devoid of band4.9, and a box-like arrangement of bands of band4.9. A dissociation between colours red (actin) and green (band4.9) occurred as well. In contrast, erythrocytes suspended in a plasma/Iodixanol-320 mixture showed a membrane cytoskeleton comparable to cells suspended in autologous plasma, Similar results were found with respect to the distribution of actin. This study revealed for the first time RCM-dependent differences in band4.9 activities as possible pathophysiological mechanism for the chemotoxicity of radiographic contrast media.
authors.affiliation: Department of Biomaterials, University of Ulm, Ulm, Germany.
authors.forename: R P
authors.initials: RP
authors.lastname: Franke
authors.affiliation: Institute for Biological Interfaces, Karlsruhe Institute of Technology (KIT) Karlsruhe, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Scharnweber
authors.affiliation: Department of Biomaterials, University of Ulm, Ulm, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Fuhrmann
authors.affiliation: Institute for Heart and Circulation Research, Hamburg, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Mrowietz
authors.affiliation: Institute for Transplantation Diagnostics and Cell Therapeutics, Medical Center of University Düsseldorf, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Wenzel
authors.affiliation: Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Berlin and Teltow, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Krüger
authors.affiliation: Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Berlin and Teltow, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Jung
mesh_terms: D000199:Actins / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000737:chemistry; D002462:Cell Membrane / Q000737:chemistry; D003287:Contrast Media / Q000737:chemistry*; D003599:Cytoskeleton / Q000737:chemistry*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D005089:Exocytosis; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006801:Humans; D007472:Iohexol / Q000031:analogs & derivatives / Q000737:chemistry; D008840:Microfilament Proteins / Q000737:chemistry*
chemical_list: D000199:Actins; D000911:Antibodies, Monoclonal; D003287:Contrast Media; C044805:DMTN protein, human; D008840:Microfilament Proteins; D007472:Iohexol; C038192:iopromide; D016650:Fluorescein-5-isothiocyanate
keywords: Iopromide-370; Radiographic contrast media; actin; band4.9 Iodixanol-320; chemotoxicity; cytoskeletal junctional complex; erythrocyte