title: Intracellular disassembly and localization of a new P123-PEI-R13/DNA complex.
abstract: The appropriate location and release of target gene is necessary for gene therapy. In our previous paper, a gene vector named P123-PEI-R13 has been successfully synthesized, and the physical characteristics and cellular trafficking of nanoparticle P123-PEI-R13/DNA has been explored explicitly, but little was known about its disassembly within cells. In order to investigate its intracellular disassembly, P123-PEI-R13/DNA complex was exposed to the different competitors (RNA, DNA, proteins) or different conditions of pH and osmolarity, DNA release was determined by gel electrophoresis. Meanwhile, confocal laser technology was used to locate the complex in cells. The results revealed that DNA, RNA and osmolarity could affect the stability of the complex obviously, especially RNA which exist in nucleus. In addition, the speed of DNA release decreased as the weight ratio of polymer increased. Images got by a confocal fluorescence microscope confirmed that after cell uptake, P123-PEI-R13 could translocate DNA into nucleus.
authors.affiliation: Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, P.R. China.
authors.forename: Manman
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, P.R. China.
authors.forename: Kehai
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, P.R. China.
authors.forename: Shunsheng
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Lv
authors.affiliation: Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, P.R. China.
authors.forename: Wenfang
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
mesh_terms: D004247:DNA / Q000008:administration & dosage / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D007097:Imines / Q000737:chemistry; D018613:Microscopy, Confocal / Q000379:methods; D057054:Molecular Imaging / Q000379:methods*; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D009842:Oligopeptides / Q000737:chemistry / Q000493:pharmacokinetics*; D010316:Particle Size; D011060:Poloxalene / Q000737:chemistry; D011095:Polyethylenes / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013347:Subcellular Fractions / Q000378:metabolism* / Q000648:ultrastructure; D014162:Transfection / Q000379:methods*
chemical_list: D007097:Imines; D053769:Nanocapsules; D009842:Oligopeptides; D011095:Polyethylenes; D011108:Polymers; C505405:poly(ethylene imine); C464484:pluronic block copolymer P123; C047981:arginyl-glycyl-aspartic acid; D011060:Poloxalene; D004247:DNA
keywords: P123-PEI-R13/DNA complex; disassembly; intracellular localization