title: Identification of genotypes of Influenza A virus in Malaysia.
abstract: OBJECTIVE
  Influenza is considered as an emerging disease until today. The present study was undertaken to determine the prevalent genotypes of Influenza A virus in Malaysia.
  
  
  METHODS
  Influenza A virus was identified from respiratory specimens by real-time reverse transcriptase polymerase chain reaction (rRT-PCR). Phylogenetic analysis of the identified isolates was performed and genotypes were detected.
  
  
  RESULTS
  A total number of 505 throat swabs and nasopharyngeal aspirates were examined by rRT-PCR at Universiti Kebangsaan Malaysia Medical Centre (UKMMC) in which 65(12.87%) were positive for influenza A. The identified isolates were successfully genotyped by phylogenetic analysis. The identified influenza A genotypes were: H1N1 (42), H3N2 (20) and H5N1 (3).
  
  
  CONCLUSIONS
  The findings indicated that 3 genotypes were circulating in Malaysia during 2011 in which H1N1 was the predominant. RESULTS added new genotype (H5N1) identification record in Malaysia that may be added in data base of WHO and CDC.
authors.affiliation: Rahman MM, Professor, Dept. of Medical Microbiology & Immunology, Faculty of Medicine, UKM, Cheras 56000, Kuala Lumpur, Malaysia.
authors.forename: Rahman
authors.initials: R
authors.lastname: Mm
authors.affiliation: Wong KK, Scholar, Dept. of Medical Microbiology & Immunology, Faculty of Medicine, UKM, Cheras 56000, Kuala Lumpur, Malaysia.
authors.forename: Wong
authors.initials: W
authors.lastname: Kk
authors.affiliation: Ilina Isahak, Professor, Faculty of Medicine and Health Sciences, University Sains Islam, Kuala Lumpur, Malaysia.
authors.forename: Isahak
authors.initials: I
authors.lastname: I
authors.affiliation: Rashid ZZ, Senior Lecturer, Dept. of Medical Microbiology & Immunology, Faculty of Medicine, UKM, Cheras 56000, Kuala Lumpur, Malaysia.
authors.forename: Rashid
authors.initials: R
authors.lastname: Zz
authors.affiliation: Alfizah H, Senior Lecturer, Dept. of Medical Microbiology & Immunology, Faculty of Medicine, UKM, Cheras 56000, Kuala Lumpur, Malaysia.
authors.forename: Alfizah
authors.initials: A
authors.lastname: H
keywords: H1N1; H3N2; H5N1; Influenza A; Phylogenetic analysis; rRT-PCR