title: Authentication of Atlantic salmon (Salmo salar) using real-time PCR.
abstract: A real-time PCR assay based on LNA TaqMan probe technology was developed for the detection and identification of Atlantic salmon (Salmo salar). Among the advantages it is worth highlighting simplicity, rapidity, highest potential for automation and minor risk of contamination of this technique. The TaqMan real-time PCR is the currently most suitable method for screening, allowing the detection of fraudulent or unintentional mislabelling of this species. The method can be applied to all kind of products, fresh, frozen and processed products, including those undergoing intensive processes of transformation. The developed methodology using specific primers-probe set was validated and further applied to 20 commercial samples labelled as salmon or S. salar in order to determinate if the species used for their manufacturing corresponded to this species. The methodology herein developed is useful to check the fulfilment of labelling regulations for seafood products, verify the correct traceability in commercial trade and for fisheries control.
authors.affiliation: Area of Molecular Biology and Biotechnology, ANFACO-CECOPESCA, Vigo, 36310 Pontevedra, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Herrero
authors.affiliation: Area of Molecular Biology and Biotechnology, ANFACO-CECOPESCA, Vigo, 36310 Pontevedra, Spain.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Vieites
authors.affiliation: Area of Molecular Biology and Biotechnology, ANFACO-CECOPESCA, Vigo, 36310 Pontevedra, Spain. Electronic address: montse@anfaco.es.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Espiñeira
keywords: Identification; PCR; Real-time PCR; Salmo salar; Salmon; TaqMan