title: A review on impedimetric biosensors.
abstract: Electrochemical impedance spectroscopy (EIS) is a sensitive technique for the analysis of the interfacial properties related to biorecognition events such as reactions catalyzed by enzymes, biomolecular recognition events of specific binding proteins, lectins, receptors, nucleic acids, whole cells, antibodies or antibody-related substances, occurring at the modified surface. Many studies on impedimetric biosensors are focused on immunosensors and aptasensors. In impedimetric immunosensors, antibodies and antigens are bound each other and thus immunocomplex is formed and the electrode is coated with a blocking layer. As a result of that electron transfer resistance increases. In impedimetric aptasensors, impedance changes following the binding of target sequences, conformational changes, or DNA damages. Impedimetric biosensors allow direct detection of biomolecular recognition events without using enzyme labels. In this paper, impedimetric biosensors are reviewed and the most interesting ones are discussed.
authors.affiliation: a Namık Kemal University, Scientific and Technological Research Center , Tekirdağ , Türkiye.
authors.forename: Elif Burcu
authors.initials: EB
authors.lastname: Bahadır
authors.affiliation: b Biochemistry Division, Chemistry Department, Faculty of Science , Namık Kemal University , Tekirdağ , Türkiye.
authors.forename: Mustafa Kemal
authors.initials: MK
authors.lastname: Sezgintürk
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D000941:Antigens / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002384:Catalysis; D058266:Dielectric Spectroscopy / Q000295:instrumentation / Q000379:methods*; D017097:Electric Impedance; D055664:Electrochemical Techniques; D004566:Electrodes; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005057:Eukaryotic Cells / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D015398:Signal Transduction
chemical_list: D000906:Antibodies; D000941:Antigens; D004800:Enzymes, Immobilized
keywords: biosensor; electrochemical impedance spectroscopy; impedance-based biosensor