title: A serum microRNA panel as potential biomarkers for hepatocellular carcinoma related with hepatitis B virus.
abstract: BACKGROUND
  The identification of new high-sensitivity and high-specificity markers for HCC are essential. We aimed to identify serum microRNAs (miRNAs) as biomarkers to be used in diagnosing hepatitis B virus (HBV) -related hepatocellular carcinoma (HCC).
  
  
  METHODS
  We investigated serum miRNA expression in (261 HCC patients, 233 cirrhosis patients, and 173 healthy controls), recruited between August 2010 and June 2013. An initial screening of miRNA expression by Illumina sequencing was performed using serum samples pooled from HCC patients and controls. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) was used to evaluate the expression of selected miRNAs. A logistic regression model was constructed using a training cohort (n = 357) and then validated using an independent cohort (n = 241). The area under the receiver operating characteristic curve (AUC) was used to evaluate the accuracy of the use of the biomarkers for disease diagnosis.
  
  
  RESULTS
  We identified 8 miRNAs (hsa-miR-206, hsa-miR-141-3p, hsa-miR-433-3p, hsa-miR-1228-5p, hsa-miR-199a-5p, hsa-miR-122-5p, hsa-miR-192-5p, and hsa-miR-26a-5p) and constructed an miRNA set that provided high diagnostic accuracy for HCC (AUC = 0.887 and 0.879 for training and validation sets, respectively). The miRNAs could also be used to differentiate HCC patients from healthy (AUC = 0.893) and cirrhosis (AUC = 0.892) patients.
  
  
  CONCLUSIONS
  We identified a serum of miRNA panel that has considerable clinical value in HCC diagnosis.
authors.affiliation: Department of Hepatosis, The Third Hospital of Zhenjiang Affiliated Jiangsu University, Zhenjiang, China; Department of Infectious Diseases, The First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Youwen
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Department of Hepatosis, The Third Hospital of Zhenjiang Affiliated Jiangsu University, Zhenjiang, China.
authors.forename: Guohong
authors.initials: G
authors.lastname: Ge
authors.affiliation: Department of Hepatosis, The Third Hospital of Zhenjiang Affiliated Jiangsu University, Zhenjiang, China.
authors.forename: Tengli
authors.initials: T
authors.lastname: Pan
authors.affiliation: Department of Hepatosis, The Third Hospital of Zhenjiang Affiliated Jiangsu University, Zhenjiang, China.
authors.forename: Danfeng
authors.initials: D
authors.lastname: Wen
authors.affiliation: Department of Hepatosis, The Third Hospital of Zhenjiang Affiliated Jiangsu University, Zhenjiang, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Hepatosis, The Third Hospital of Zhenjiang Affiliated Jiangsu University, Zhenjiang, China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Hepatosis, The Third Hospital of Zhenjiang Affiliated Jiangsu University, Zhenjiang, China.
authors.forename: Xinbei
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Infectious Diseases, The First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Jianhe
authors.initials: J
authors.lastname: Gan
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D016022:Case-Control Studies; D006509:Hepatitis B / Q000150:complications*; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000821:virology; D016015:Logistic Models; D035683:MicroRNAs / Q000097:blood*
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs