title: A droplet-based fluorescence polarization immunoassay (dFPIA) platform for rapid and quantitative analysis of biomarkers.
abstract: Herein, we describe for the first time the integration of pneumatic micro-pumps with droplet-based microfluidic systems as basic platform for the rapid detection and quantitation of biomarkers. Specifically, we combine this microfluidic platform with fluorescence polarization detection to identify and quantify the potent blood vessel inducing protein bovine angiogenin within cow's milk in high-throughput. The droplet-based fluorescence polarization immunoassay is successful in accurately determining the concentration (4.84±1.21 μg/mL) of bovine angiogenin in cow's milk, affords a 10 fold reduction in dead volumes when compared to conventional droplet-based microfluidic experiments and requires an total sample volume of less than 1 nL.
authors.affiliation: Department of Biochemistry, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Choi
authors.affiliation: Department of Biochemistry, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Gil-Jung
authors.initials: GJ
authors.lastname: Kim
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.
authors.forename: Sangmin
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.
authors.forename: Joonwon
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Chemistry and Applied Biosciences, ETH Zürich, Vladimir Prelog Weg 1, Zürich CH-8093, Switzerland.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: deMello
authors.affiliation: Department of Biochemistry, Chungbuk National University, Cheongju 361-763, Republic of Korea. Electronic address: sichang@chungbuk.ac.kr.
authors.forename: Soo-Ik
authors.initials: SI
authors.lastname: Chang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood* / Q000737:chemistry; D015374:Biosensing Techniques; D001808:Blood Vessels / Q000378:metabolism; D002417:Cattle; D016231:Fluorescence Polarization Immunoassay; D006801:Humans; D046210:Microfluidic Analytical Techniques; D008892:Milk / Q000737:chemistry; D012259:Ribonuclease, Pancreatic / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D015415:Biomarkers; C046366:angiogenin; D012259:Ribonuclease, Pancreatic
keywords: Angiogenin; Biomarker; Droplet-based microfluidics; Fluorescence polarization; Immunoassay; Milk