title: Characterization of stem-like cells in mucoepidermoid tracheal paediatric tumor.
abstract: Stem cells contribute to regeneration of tissues and organs. Cells with stem cell-like properties have been identified in tumors from a variety of origins, but to our knowledge there are yet no reports on tumor-related stem cells in the human upper respiratory tract. In the present study, we show that a tracheal mucoepidermoid tumor biopsy obtained from a 6 year-old patient contained a subpopulation of cells with morphology, clonogenicity and surface markers that overlapped with bone marrow mesenchymal stromal cells (BM-MSCs). These cells, designated as MEi (mesenchymal stem cell-like mucoepidermoid tumor) cells, could be differentiated towards mesenchymal lineages both with and without induction, and formed spheroids in vitro. The MEi cells shared several multipotent characteristics with BM-MSCs. However, they displayed differences to BM-MSCs in growth kinectics and gene expression profiles relating to cancer pathways and tube development. Despite this, the MEi cells did not possess in vivo tumor-initiating capacity, as proven by the absence of growth in situ after localized injection in immunocompromised mice. Our results provide an initial characterization of benign tracheal cancer-derived niche cells. We believe that this report could be of importance to further understand tracheal cancer initiation and progression as well as therapeutic development.
authors.affiliation: Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose, Throat, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Mei Ling
authors.initials: ML
authors.lastname: Lim
authors.affiliation: School of Applied Science, Republic Polytechnic, Singapore.
authors.forename: Brandon Nick Sern
authors.initials: BN
authors.lastname: Ooi
authors.affiliation: Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose, Throat, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Philipp
authors.initials: P
authors.lastname: Jungebluth
authors.affiliation: Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose, Throat, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Sjöqvist
authors.affiliation: Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Isabell
authors.initials: I
authors.lastname: Hultman
authors.affiliation: Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose, Throat, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Greg
authors.initials: G
authors.lastname: Lemon
authors.affiliation: Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose, Throat, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Ylva
authors.initials: Y
authors.lastname: Gustafsson
authors.affiliation: Department of Oncology and Pathology, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Jurate
authors.initials: J
authors.lastname: Asmundsson
authors.affiliation: Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose, Throat, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Silvia
authors.initials: S
authors.lastname: Baiguera
authors.affiliation: Center for Hematology and Regenerative Medicine, Department of Medicine, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Iyadh
authors.initials: I
authors.lastname: Douagi
authors.affiliation: International Scientific-Research Clinical and Educational Center of Regenerative Medicine, Kuban State Medical University, Krasnodar, Russian Federation.
authors.forename: Irina
authors.initials: I
authors.lastname: Gilevich
authors.affiliation: International Scientific-Research Clinical and Educational Center of Regenerative Medicine, Kuban State Medical University, Krasnodar, Russian Federation.
authors.forename: Alina
authors.initials: A
authors.lastname: Popova
authors.affiliation: Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose, Throat, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Johannes Cornelius
authors.initials: JC
authors.lastname: Haag
authors.affiliation: Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose, Throat, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Antonio Beltrán
authors.initials: AB
authors.lastname: Rodríguez
authors.affiliation: Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose, Throat, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Jianri
authors.initials: J
authors.lastname: Lim
authors.affiliation: Department of Clinical Genetics, Karolinska University Hospital, Stockholm, Sweden; Department of Molecular Medicine and Surgery and Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Agne
authors.initials: A
authors.lastname: Liedén
authors.affiliation: Department of Clinical Genetics, Karolinska University Hospital, Stockholm, Sweden; Department of Molecular Medicine and Surgery and Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Magnus
authors.initials: M
authors.lastname: Nordenskjöld
authors.affiliation: Center for Hematology and Regenerative Medicine, Department of Medicine, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Evren
authors.initials: E
authors.lastname: Alici
authors.affiliation: Department of Biomedical Sciences, University of Sheffield, Sheffield, United Kingdom.
authors.forename: Duncan
authors.initials: D
authors.lastname: Baker
authors.affiliation: Department of Biomedical Sciences, University of Sheffield, Sheffield, United Kingdom.
authors.forename: Christian
authors.initials: C
authors.lastname: Unger
authors.affiliation: Department of Medicine III, University Hospital RWTH Aachen, Germany.
authors.forename: Tom
authors.initials: T
authors.lastname: Luedde
authors.affiliation: Robinson Institute, Center for Stem Cell Research, The University of Adelaide, Adelaide, Australia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Vassiliev
authors.affiliation: Department of Biosciences and Nutrition, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden.
authors.forename: Jose
authors.initials: J
authors.lastname: Inzunza
authors.affiliation: Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Lars
authors.initials: L
authors.lastname: Ahrlund-Richter
authors.affiliation: Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of Ear, Nose, Throat, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Paolo
authors.initials: P
authors.lastname: Macchiarini
mesh_terms: D000818:Animals; D002469:Cell Separation; D002648:Child; D005260:Female; D020869:Gene Expression Profiling; D023281:Genomics; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000473:pathology; D051379:Mice; D018298:Mucoepidermoid Tumor / Q000175:diagnosis / Q000235:genetics / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000473:pathology*; D014134:Tracheal Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000473:pathology*