title: Separation and determination of 4-methylimidazole, 2-methylimidazole and 5-hydroxymethylfurfural in beverages by amino trap column coupled with pulsed amperometric detection.
abstract: A method for simultaneous determination of 4-methylimidazole (4-MeI), 2-methylimidazole (2-MeI) and 5-hydroxymethylfurfural (HMF) in beverages was developed using solid-phase extraction (SPE) and amino trap column coupled with pulsed amperometric detection (AMTC-PAD). A single amino trap column (P/N: 046122) was first applied to separate the targeted analytes in samples after SPE pretreatment. This method demonstrated low limit of quantification (0.030mg/L for methylimidazoles and 0.300mg/L for HMF) and excellent linearity with correlation of determination (R(2)=0.999 for 2-MeI, 0.997 for 4-MeI and 0.998 for HMF). Nearly no 2-MeI was found in all soft drinks. However, 4-MeI could be detected in cola drinks and soft drinks containing caramel colour (ranging from 0.13 to 0.34mg/L), whereas HMF were only found in cola drinks (ranging from 1.07 to 4.47mg/L). Thus, AMTC-PAD technique would be a valid and inexpensive alternative to analysis of 4-MeI, 2-MeI and HMF.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Xian-Bing
authors.initials: XB
authors.lastname: Xu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Ding-Bo
authors.initials: DB
authors.lastname: Liu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China; State Key Laboratory of Pulp and Paper Engineering, Guangzhou 510640, China. Electronic address: shujuanyu8@gmail.com.
authors.forename: Shu-Juan
authors.initials: SJ
authors.lastname: Yu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Yu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Zhen-Gang
authors.initials: ZG
authors.lastname: Zhao
mesh_terms: D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D055664:Electrochemical Techniques; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis; D007093:Imidazoles / Q000032:analysis*; D052616:Solid Phase Extraction
chemical_list: D007093:Imidazoles; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde; C023439:4-methylimidazole; C032655:2-methylimidazole
keywords: 2-Methylimidazole; 4-Methylimidazole; 5-Hydroxymethylfurfural; AMTC-PAD; Beverage; Caramelization products; Solid-phase extraction