title: Zinc-based fixation for high-sensitivity in situ hybridization: a nonradioactive colorimetric method for the detection of rare transcripts on tissue sections.
abstract: Nonradioactive colorimetric in situ hybridization (NoRISH) has been widely applied to analyze gene expression at the single-cell level. Zinc fixation is time efficient and provides excellent tissue morphology. Furthermore, it improves the preservation of the RNA, facilitating the detection of rare transcripts or the identification of expressing cells scattered within a tissue. Here we present a rapid, highly sensitive NoRISH method that uses a zinc-salt-based fixative and is especially suitable for the study of genes expressed at low levels and/or in a small number of cells within a structure.
authors.affiliation: Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis, Greece.
authors.forename: Electra
authors.initials: E
authors.lastname: Stylianopoulou
authors.forename: George
authors.initials: G
authors.lastname: Skavdis
authors.forename: Maria
authors.initials: M
authors.lastname: Grigoriou
mesh_terms: D000818:Animals; D003124:Colorimetry / Q000191:economics / Q000379:methods; D005404:Fixatives / Q000737:chemistry*; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization / Q000191:economics / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D016707:Tissue Fixation / Q000379:methods*; D014158:Transcription, Genetic; D015032:Zinc / Q000737:chemistry*
chemical_list: D005404:Fixatives; D012333:RNA, Messenger; D015032:Zinc