title: Application of graphene-copper sulfide nanocomposite modified electrode for electrochemistry and electrocatalysis of hemoglobin.
abstract: In this paper a graphene (GR) and copper sulfide (CuS) nanocomposite was synthesized by hydrothermal method and used for the electrode modification with a N-butylpyridinium hexafluorophosphate based carbon ionic liquid electrode (CILE) as the substrate electrode. Hemoglobin (Hb) was immobilized on the modified electrode to get a biocompatible sensing platform. UV-vis absorption spectroscopic results confirmed that Hb retained its native secondary structure in the composite. Direct electron transfer of Hb incorporated into the nanocomposite was investigated with a pair of well-defined redox waves appeared on cyclic voltammogram, indicating the realization of direct electrochemistry of Hb on the modified electrode. The results can be ascribed to the presence of GR-CuS nanocomposite on the electrode surface that facilitates the electron transfer rate between the electroactive center of Hb and the electrode. The Hb modified electrode showed excellent electrocatalytic activity to the reduction of trichloroacetic acid in the concentration range from 3.0 to 64.0 mmol L(-1) with the detection limit of 0.20 mmol L(-1) (3σ). The fabricated biosensor displayed the advantages such as high sensitivity, good reproducibility and long-term stability.
authors.affiliation: College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Shi
authors.affiliation: College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China.
authors.forename: Weizhe
authors.initials: W
authors.lastname: Zheng
authors.affiliation: College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China.
authors.forename: Wencheng
authors.initials: W
authors.lastname: Wang
authors.affiliation: College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Hou
authors.affiliation: College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China.
authors.forename: Bingxin
authors.initials: B
authors.lastname: Lei
authors.affiliation: College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China.
authors.forename: Zhenfan
authors.initials: Z
authors.lastname: Sun
authors.affiliation: College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China. Electronic address: swyy26@hotmail.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
mesh_terms: D002384:Catalysis; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D003217:Conductometry / Q000295:instrumentation*; D003300:Copper / Q000737:chemistry*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry*; D006454:Hemoglobins / Q000032:analysis* / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D013440:Sulfides / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D006454:Hemoglobins; D052578:Ionic Liquids; D013440:Sulfides; D006108:Graphite; D003300:Copper
keywords: Carbon ionic liquid electrode; Copper sulfide; Direct electrochemistry; Electrocatalysis; Graphene; Hemoglobin