title: Oriented matrix promotes directional tubulogenesis.
abstract: Detailed control over the structural organization of scaffolds and engineered tissue constructs is a critical need in the quest to engineer functional tissues using biomaterials. This work presents a new approach to spatially direct endothelial tubulogenesis. Micropatterned fibronectin substrates were used to control lung fibroblast adhesion and growth and the subsequent deposition of fibroblast-derived matrix during culture. The fibroblast-derived matrix produced on the micropatterned substrates was tightly oriented by these patterns, with an average variation of only 8.5°. Further, regions of this oriented extracellular matrix provided directional control of developing endothelial tubes to within 10° of the original micropatterned substrate design. Endothelial cells seeded directly onto the micropatterned substrate did not form tubes. A metric for matrix anisotropy showed a relationship between the fibroblast-derived matrix and the endothelial tubes that were subsequently developed on the same micropatterns with a resulting aspect ratio over 1.5 for endothelial tubulogenesis. Micropatterns in "L" and "Y" shapes were used to direct endothelial tubes to turn and branch with the same level of precision. These data demonstrate that anisotropic fibroblast-derived matrices instruct the alignment and shape of endothelial tube networks, thereby introducing an approach that could be adapted for future design of microvascular implants featuring organ-specific natural matrix that patterns microvascular growth.
authors.affiliation: Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Bioengineering, University of Louisville, Louisville, KY 40292, USA.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Soucy
authors.affiliation: Intelligent Substrates, Inc., Sykesville, MD, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Hoh
authors.affiliation: Intelligent Substrates, Inc., Sykesville, MD, USA; Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Will
authors.initials: W
authors.lastname: Heinz
authors.affiliation: Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Jan
authors.initials: J
authors.lastname: Hoh
authors.affiliation: Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Anesthesiology and Critical Care Medicine, Cell Biology, and Pediatrics, and the Center for Cell Dynamics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Lewis
authors.initials: L
authors.lastname: Romer
mesh_terms: D016880:Anisotropy; D040761:Biomimetic Materials / Q000138:chemical synthesis; D002460:Cell Line; D016764:Cell Polarity; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005109:Extracellular Matrix / Q000737:chemistry* / Q000648:ultrastructure; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D048091:Guided Tissue Regeneration / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008422:Materials Testing; D055806:Microvessels / Q000166:cytology* / Q000254:growth & development*; D018919:Neovascularization, Physiologic / Q000502:physiology; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds
keywords: Anisotropy; Endothelial morphogenesis; Extracellular matrix; Micropattern substrate