title: Novel strategy to release and tag N-glycans for functional glycomics.
abstract: Functional glycomics has been impeded by the lack of inexpensive enzymatic and mild chemical methods to acquire natural glycans in significant amounts. In this study, we have developed a new strategy we term "threshing and trimming" (TaT) to quickly obtain N-glycans from glycoproteins and animal tissues. TaT employs low-cost Pronase to degrade peptides and N-bromosuccinimide (NBS) to effect oxidative decarboxylation under very mild reaction conditions to generate homogeneous aglycon moieties as nitriles or aldehydes. These aglycons can be readily conjugated with fluorescent tags for profiling and functional study. TaT is an affordable alternative to expensive specialty enzymes and strong chemical treatment and unpleasant reagents, and should further drive the functional glycomics of N-glycans.
authors.affiliation: Department of Biochemistry Emory University School of Medicine Atlanta, Georgia 30322, United States.
authors.forename: Xuezheng
authors.initials: X
authors.lastname: Song
authors.forename: Hong
authors.initials: H
authors.lastname: Ju
authors.forename: Chunmei
authors.initials: C
authors.lastname: Zhao
authors.forename: Yi
authors.initials: Y
authors.lastname: Lasanajak
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D054794:Glycomics / Q000379:methods*; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000032:analysis* / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D011134:Polysaccharides