title: Is ozone pre-conditioning effect linked to Nrf2/EpRE activation pathway in vivo? A preliminary result.
abstract: The present preliminary study has been focused on verifying whether ozone preconditioning may be linked to Nrf2/EpRE (nuclear factor erythroid 2/electrophile-responsive element) activation pathway in vivo. Healthy volunteers received a total of three Major Auto-Hemotherapy (MAH) treatments, with treatments administered every second day. The amount of blood used for each subject was standardized to the value obtained multiplying the subject׳s body weight by 1.3 in order to ensure the same ozone concentrations for each subject. A parallel group (n=50) age and gender matched was used as reference for the experimental variables related to the oxidative stress parameters. Levels of Nrf2 and oxidative stress index were measured throughout the study. Levels of Nrf2 (P<0.01) in peripheral blood mononuclear cells (PBMC) were found to increase immediately after ozone/oxygen exposure (35µg/ml, prior to reinfusion). This effect was still detected (P<0.05) in total circulating PBMC when measured 30min following reinfusion. After a series of 3 MAH, Nrf2 returned back to the basal level. At the end of the experiment the activities of superoxide dismutase and catalase were increased (P<0.05). These data demonstrate for the first time in vivo the activation of the Nrf2 pathway by a low dose of ozone and the promotion of the feedback mechanism that induces the synthesis of proteins which collectively favors cell survival.
authors.affiliation: Clinical Pharmacology & Toxicology Department, Medinat SAS, Via Fazioli 22, 60021 Camerano, Ancona, Italy. Electronic address: lambertore@lambertore.com.
authors.forename: Lamberto
authors.initials: L
authors.lastname: Re
authors.affiliation: Medical Center Beauty Benefit - San Biagio di Osimo, Via Mons. Oscar Romero, 31, 60027 Osimo, Ancona, Italy.
authors.forename: Gregorio
authors.initials: G
authors.lastname: Martínez-Sánchez
authors.affiliation: Department of Internal Medicine, University of Ancona, Politecnica delle Marche, 60131 Ancona, Italy.
authors.forename: Marica
authors.initials: M
authors.lastname: Bordicchia
authors.affiliation: Clinical Pharmacology & Toxicology Department, Medinat SAS, Via Fazioli 22, 60021 Camerano, Ancona, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Malcangi
authors.affiliation: Department of Internal Medicine, University of Ancona, Politecnica delle Marche, 60131 Ancona, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Pocognoli
authors.affiliation: Department of Pharmacology, Faculty of Medicine, University of Chile, Santiago de Chile, Chile.
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Morales-Segura
authors.affiliation: Holistic Dentist Clinic, 175 Mercado Street, Suite 115, Durango, CO 81301, USA.
authors.forename: John
authors.initials: J
authors.lastname: Rothchild
authors.affiliation: Biomedical Research Laboratories, Medicine Faculty, Catholic University of Maule, Talca, Chile.
authors.forename: Armando
authors.initials: A
authors.lastname: Rojas
mesh_terms: D000328:Adult; D061745:Antioxidant Response Elements / Q000187:drug effects*; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D008297:Male; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D051267:NF-E2-Related Factor 2 / Q000097:blood*; D018384:Oxidative Stress / Q000187:drug effects; D010126:Ozone / Q000008:administration & dosage* / Q000627:therapeutic use; D011446:Prospective Studies
chemical_list: D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; D010126:Ozone
keywords: Autohemotherapy; Electrophile-responsive element; EpRE; Nrf2; Nuclear factor erythroid 2; Ozone therapy