title: Evaluation of okadaic acid, dinophysistoxin-1 and dinophysistoxin-2 toxicity on Neuro-2a, NG108-15 and MCF-7 cell lines.
abstract: Marine dinoflagelates from the genus Dynophisis are important producers of Diarrhetic Shellfish Poisoning (DSP) toxins which are responsible for human intoxications. The present work is an approach to study the relative toxicity of DSP toxins effects on Neuro-2a, NG108-15 and MCF-7 cell-lines. Certified standards of okadaic acid (OA), dinophysistoxin-1 (DTX-1) and dinophysistoxin-2 (DTX-2) were used. Our results show that the three toxins exhibit similar cytotoxicity in Neuro-2a and NG108-15 cell lines. Conversely, MCF-7 cells were the least sensitive to these toxins. DTX-1 displayed the most toxic effect in the three tested cell lines.
authors.affiliation: IRTA, Marine Monitoring Subprogram, Ctra. Poble Nou, Km 5.5, 43540 Sant Carles de la Ràpita, Tarragona, Spain; Universitat Rovira i Virgili, Faculty of Medicine & Health Sciences, Pharmacology Unit, c./ St. Llorenç 21, 43201 Reus, Tarragona, Spain.
authors.forename: Lucia
authors.initials: L
authors.lastname: Soliño
authors.affiliation: Universitat Rovira i Virgili, Faculty of Medicine & Health Sciences, Pharmacology Unit, c./ St. Llorenç 21, 43201 Reus, Tarragona, Spain.
authors.forename: Francesc X
authors.initials: FX
authors.lastname: Sureda
authors.affiliation: IRTA, Marine Monitoring Subprogram, Ctra. Poble Nou, Km 5.5, 43540 Sant Carles de la Ràpita, Tarragona, Spain. Electronic address: jorge.diogene@irta.cat.
authors.forename: Jorge
authors.initials: J
authors.lastname: Diogène
mesh_terms: D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006801:Humans; D061986:MCF-7 Cells / Q000187:drug effects; D008387:Marine Toxins / Q000633:toxicity*; D019319:Okadaic Acid / Q000633:toxicity* / Q000031:analogs & derivatives; D011714:Pyrans / Q000633:toxicity*
chemical_list: D008387:Marine Toxins; D011714:Pyrans; D019319:Okadaic Acid; C051904:dinophysistoxin 1; C076221:dinophysistoxin 2
keywords: Cell-based assay (CBA); Dinophysistoxin-1 (DTX-1); Dinophysistoxin-2 (DTX-2); Marine toxins; Okadaic acid (OA)