title: Organ-specific effects of brassinosteroids on stomatal production coordinate with the action of Too Many Mouths.
abstract: In Arabidopsis, stomatal development initiates after protodermal cells acquire stomatal lineage cell fate. Stomata or their precursors communicate with their neighbor epidermal cells to ensure the "one cell spacing" rule. The signals from EPF/EPFL peptide ligands received by Too Many Mouths (TMM) and ERECTA-family receptors are supposed to be transduced by YODA MAPK cascade. A basic helix-loop-helix transcription factor SPEECHLESS (SPCH) is another key regulator of stomatal cell fate determination and asymmetric entry divisions, and SPCH activity is regulated by YODA MAPK cascade. Brassinosteroid (BR) signaling, one of the most well characterized signal transduction pathways in plants, contributes to the control of stomatal production. But opposite organ-specific effects of BR on stomatal production were reported. Here we confirm that stomatal production in hypocotyls is controlled by BR levels. YODA and CYCD4 are not essential for BR stomata-promoting function. Furthermore, we found that BR could confer tmm hypocotyls clustered stomatal phenotype, indicating that the BR organ-specific effects on stomatal production might coordinate with the TMM organ-specific actions.
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Beijing, 100093, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
authors.forename: Kezhen
authors.initials: K
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Le
mesh_terms: D029681:Arabidopsis Proteins / Q000378:metabolism*; D060406:Brassinosteroids / Q000494:pharmacology*; D018546:Hypocotyl / Q000187:drug effects / Q000378:metabolism; D009928:Organ Specificity / Q000187:drug effects; D010641:Phenotype; D054046:Plant Stomata / Q000187:drug effects / Q000254:growth & development*; D055550:Protein Stability / Q000187:drug effects
chemical_list: D029681:Arabidopsis Proteins; D060406:Brassinosteroids; C459090:too many mouths protein, Arabidopsis
keywords: Arabidopsis; brassinosteroids; development; stomata