title: Fluorescence imaging of Evans blue extravasation into mouse brain induced by low frequency ultrasound with microbubble.
abstract: Blood-Brain Barrier (BBB) can be opened locally, noninvasively and reversibly by low frequency focused ultra-sound (FUS) in the presence of microbubbles. In this study, Evans blue (EB) dye extravasation across BBB was enhanced by 1 MHz FUS at acoustic pressure of 0.35 MPa in the presence of microbubbles at clinically comparable dosage. The spatial distribution of EB extravasation was visualized using fluorescence imaging method. The center region of BBB disruption area showed more enhanced fluorescence signal than the surrounding region in general. However, EB dye deposition was heterogeneous in the center region. The findings in this study indicated potential use of fluorescence imaging to evaluate large molecules delivery across BBB.
authors.affiliation: Department of Biomedical Engineering, Shenzhen University, Shenzhen 518060, China Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Shenzhen 518060, China National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, Shenzhen 518060, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Shen
authors.affiliation: School of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200240 , China.
authors.forename: Aili
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Engineering, Shenzhen University, Shenzhen 518060, China.
authors.forename: Jinxuan
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Biomedical Engineering, Shenzhen University, Shenzhen 518060, China Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Shenzhen 518060, China National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, Shenzhen 518060, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Dan
authors.affiliation: Department of Biomedical Engineering, Shenzhen University, Shenzhen 518060, China Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Shenzhen 518060, China National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, Shenzhen 518060, China.
authors.forename: Siping
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Biomedical Engineering, Shenzhen Polytechnic, Shenzhen 518055, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000737:chemistry* / Q000528:radiation effects*; D004058:Diffusion; D005070:Evans Blue / Q000737:chemistry*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D045423:Microbubbles; D008856:Microscopy, Fluorescence / Q000379:methods*; D013010:Sonication / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D005070:Evans Blue
keywords: Blood-Brain Barrier disruption; Evans blue; Fluorescence imaging; Focused Ultrasound; microbubble