title: Development of the automated circulating tumor cell recovery system with microcavity array.
abstract: Circulating tumor cells (CTCs) are well recognized as useful biomarker for cancer diagnosis and potential target of drug discovery for metastatic cancer. Efficient and precise recovery of extremely low concentrations of CTCs from blood has been required to increase the detection sensitivity. Here, an automated system equipped with a microcavity array (MCA) was demonstrated for highly efficient and reproducible CTC recovery. The use of MCA allows selective recovery of cancer cells from whole blood on the basis of differences in size between tumor and blood cells. Intra- and inter-assays revealed that the automated system achieved high efficiency and reproducibility equal to the assay manually performed by well-trained operator. Under optimized assay workflow, the automated system allows efficient and precise cell recovery for non-small cell lung cancer cells spiked in whole blood. The automated CTC recovery system will contribute to high-throughput analysis in the further clinical studies on large cohort of cancer patients.
authors.affiliation: Division of Biotechnology and Life science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Negishi
authors.affiliation: Division of Biotechnology and Life science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Masahito
authors.initials: M
authors.lastname: Hosokawa
authors.affiliation: Division of Biotechnology and Life science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Seita
authors.initials: S
authors.lastname: Nakamura
authors.affiliation: Hitachi Chemical Co., Ltd., 1-9-2, Marunouchi, Chiyoda, Tokyo 100-6606, Japan.
authors.forename: Hisashige
authors.initials: H
authors.lastname: Kanbara
authors.affiliation: KOMEYA Professional engineer Japan office, 5-11-30, Kugayama, Suginami-ku, Tokyo 186-0081, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Kanetomo
authors.affiliation: Hitachi Chemical Co., Ltd., 1-9-2, Marunouchi, Chiyoda, Tokyo 100-6606, Japan.
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Kikuhara
authors.affiliation: Division of Biotechnology and Life science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Division of Biotechnology and Life science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Matsunaga
authors.affiliation: Division of Biotechnology and Life science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan. Electronic address: y-tomoko@cc.tuat.ac.jp.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yoshino
mesh_terms: D015374:Biosensing Techniques; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000473:pathology; D002469:Cell Separation; D058948:Cell Tracking; D006801:Humans; D046210:Microfluidic Analytical Techniques; D009360:Neoplastic Cells, Circulating
keywords: Automated system; Circulating tumor cells; Lung cancer; Whole blood