title: Effects of hyperbaric oxygen therapy as hepatic preconditioning in rats submitted to hepatic ischemia/reperfusion injury.
abstract: OBJECTIVE
  To analyze the role of hyperbaric oxygen therapy as hepatic preconditioning in rats submitted to hepatic ischemia and reperfusion.
  
  
  METHODS
  Wistar rats were randomly divided into three groups: SHAM, rats submitted to surgical stress without hepatic ischemia and reperfusion, I/R, rats submitted to total hepatic pedicle ischemia for 30 min, followed by 5 min of reperfusion; HBOI/R, rats submitted to 60 minutes of hyperbaric oxygen therapy at 2 atm and immediately submitted to the experimental protocol of ischemia and reperfusion. Liver function was assessed by measuring serum alanine aminotransferase and aspartate aminotransferase, as well as mitochondrial function by determining states 3 and 4 of mitochondrial respiration, respiratory control rate and mitochondrial permeability transition (mitochondrial swelling). The results were analyzed by the Mann-Whitney test and all P-values <0.05 were considered significant.
  
  
  RESULTS
  There were significant differences in serum aspartate aminotransferase values in groups SHAM vs. HBOI/R, I/R vs HBOI/R, alanine aminotransferase in groups SHAM and I/R; State 3 in SHAM groups vs. I/R, SHAM vs. HBOI/R, State 4 in I/R vs HBOI/R groups, respiratory control rate in SHAM vs I/R groups; mitochondrial swelling in SHAM vs. I/R groups, and SHAM vs HBOI/R.
  
  
  CONCLUSIONS
  Hyperbaric preconditioning improved hepatic mitochondrial function and decreased serum markers of liver injury in the ischemia and reperfusion process.
authors.affiliation: FAMEMA, Marília, SP.
authors.forename: Daniele Moraes
authors.initials: DM
authors.lastname: Losada
authors.affiliation: Department of Pharmacology, FAMEMA, Marília, SP.
authors.forename: Agnaldo Bruno
authors.initials: AB
authors.lastname: Chies
authors.affiliation: Ribeirão Preto Medical School, University of São Paulo.
authors.forename: Omar
authors.initials: O
authors.lastname: Feres
authors.affiliation: Medical School, Faculty of Medicine, São Paulo University, Brazil.
authors.forename: Eleazar
authors.initials: E
authors.lastname: Chaib
authors.affiliation: Medical School, Faculty of Medicine, São Paulo University, Brazil.
authors.forename: Luiz Augusto Carneiro
authors.initials: LA
authors.lastname: D'Albuquerque
authors.affiliation: Department Surgery and Anatomy.
authors.forename: Orlando
authors.initials: O
authors.lastname: Castro-e-Silva
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D019069:Cell Respiration; D006931:Hyperbaric Oxygenation / Q000379:methods*; D019194:Ischemic Preconditioning / Q000379:methods*; D008099:Liver / Q000098:blood supply* / Q000502:physiology; D008297:Male; D008930:Mitochondria, Liver / Q000502:physiology*; D010101:Oxygen Consumption; D011897:Random Allocation; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D015203:Reproducibility of Results; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase