title: Bacterial communities in the gut and reproductive organs of Bactrocera minax (Diptera: Tephritidae) based on 454 pyrosequencing.
abstract: The citrus fruit fly Bactrocera minax is associated with diverse bacterial communities. We used a 454 pyrosequencing technology to study in depth the microbial communities associated with gut and reproductive organs of Bactrocera minax. Our dataset consisted of 100,749 reads with an average length of 400 bp. The saturated rarefaction curves and species richness indices indicate that the sampling was comprehensive. We found highly diverse bacterial communities, with individual sample containing approximately 361 microbial operational taxonomic units (OTUs). A total of 17 bacterial phyla were obtained from the flies. A phylogenetic analysis of 16S rDNA revealed that Proteobacteria was dominant in all samples (75%-95%). Actinobacteria and Firmicutes were also commonly found in the total clones. Klebsiella, Citrobacter, Enterobacter, and Serratia were the major genera. However, bacterial diversity (Chao1, Shannon and Simpson indices) and community structure (PCA analysis) varied across samples. Female ovary has the most diverse bacteria, followed by male testis, and the bacteria diversity of reproductive organs is richer than that of the gut. The observed variation can be caused by sex and tissue, possibly to meet the host's physiological demands.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Institute of Urban and Horticultural Pests, and Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Ailin
authors.initials: A
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Institute of Urban and Horticultural Pests, and Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Institute of Urban and Horticultural Pests, and Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Institute of Urban and Horticultural Pests, and Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology*; D015723:Gene Library; D014644:Genetic Variation; D005835:Genitalia / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D025341:Principal Component Analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012098:Reproduction; D017422:Sequence Analysis, DNA / Q000379:methods*; D033621:Tephritidae / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S