title: Effect of legume seed extracts on the inhibition of proteolytic activity and muscle degradation of fresh water prawn (Macrobrachiumrosenbergii).
abstract: Trypsin inhibitors in the extracts from soybean (Glycine max), adzuki bean (Vigna angularis), bambara groundnut (Vigna subterranea) and red kidney bean (Phaseoulus vulgaris) varied in amount and molecular weight. The soybean extract had the highest level of trypsin inhibitor with molecular weight (MW) of 21kDa, followed by bambara groundnut extract possessing trypsin inhibitor with MW of 15kDa. Both extracts showed a more effective inhibition towards crude protease extract (CE) from the hepatopancreas of fresh water prawn (Macrobrachium rosenbergii) than the extracts from adzuki and red kidney beans. Activity staining also reconfirmed the higher inhibitory activity on CE from hepatopancreas by the extracts from both soybean and bambara groundnut. The extracts from all seeds were able to inhibit the degradation of fresh water prawn meat containing CE in a concentration dependent manner. Based on inhibitor study, the extracts from soybean and bambara groundnut can be a potential aid to suppress the muscle softening of fresh water prawn, mediated by trypsin-like proteases released from hepatopancreas, during extended iced storage.
authors.affiliation: Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
authors.forename: Chodsana
authors.initials: C
authors.lastname: Sriket
authors.affiliation: Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand. Electronic address: soottawat.b@psu.ac.th.
authors.forename: Soottawat
authors.initials: S
authors.lastname: Benjakul
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Phaholyothin Rd., Klong 1, Klong Luang, Pathumthani 12120, Thailand.
authors.forename: Wonnop
authors.initials: W
authors.lastname: Visessanguan
authors.affiliation: Graduate School of Fisheries and Environment Studies, Nagasaki University, Bunkyo, Nagasaki 852-8521, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Hara
keywords: Collagenase; Degradation; Fresh water prawn; Legume seed; Muscle proteins; Protease; Trypsin inhibitor