title: Nesterenkonia rhizosphaerae sp. nov., an alkaliphilic actinobacterium isolated from rhizosphere soil in a saline-alkaline desert.
abstract: An alkaliphilic actinobacterial strain, designated EGI 80099(T), was isolated from a rhizosphere soil sample of Reaumuria soongorica found in the desert soils of Fukang, Xinjiang, north-west China. Cells of strain EGI 80099(T) were Gram-stain-positive, non-motile, non-endospore-forming cocci. The predominant menaquinones were MK-7, MK-8 and MK-9. The major cellular fatty acids (>10 %) were anteiso-C15 : 0 and anteiso-C17 : 0. Analysis of the cell wall showed the presence of peptidoglycan of the type l-Lys-Gly-l-Glu, variation A4α. Cells of the isolate contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unknown phospholipid and an unidentified glycolipid as polar lipids. The genomic DNA G+C content was 63.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain EGI 80099(T) belongs to the genus Nesterenkonia, sharing 95.68-97.37 % sequence similarities with the type strains of recognized species within this genus. DNA-DNA hybridization of strain EGI 80099(T) with the type strains of species that showed the highest sequence similarities, Nesterenkonia aethiopica DSM 17733(T) (97.37 %), Nesterenkonia flava CAAS 251(T) (97.23 %) and Nesterenkonia xinjiangensis YIM 70097(T) (97.02 %), gave relatedness values of 8.7-62.2 %. Data from DNA-DNA hybridizations and physiological and biochemical tests indicated that strain EGI 80099(T) represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia rhizosphaerae sp. nov. is proposed. The type strain is EGI 80099(T) ( = BCRC 16947(T) = JCM 19129(T)).
authors.affiliation: University of Chinese Academy of Sciences, Beijing 100049, PR China.|Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi 830011, PR China.
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Biocontrol, Key Laboratory of Biodiversity Dynamics and Conservation of Guangdong Higher Education Institutes, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou 510275, PR China.|Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi 830011, PR China.
authors.forename: Yong-Guang
authors.initials: YG
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi 830011, PR China.
authors.forename: Ji-Yue
authors.initials: JY
authors.lastname: Chen
authors.affiliation: Bioproducts Research Chair (BRC), College of Science, King Saud University, P.O. Box 2455 Riyadh 11451, Saudi Arabia.
authors.forename: Wael N
authors.initials: WN
authors.lastname: Hozzein
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi 830011, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Bioproducts Research Chair (BRC), College of Science, King Saud University, P.O. Box 2455 Riyadh 11451, Saudi Arabia.
authors.forename: Mohammed A M
authors.initials: MAM
authors.lastname: Wadaan
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi 830011, PR China.
authors.forename: Yuan-Ming
authors.initials: YM
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.|State Key Laboratory of Biocontrol, Key Laboratory of Biodiversity Dynamics and Conservation of Guangdong Higher Education Institutes, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou 510275, PR China.|Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi 830011, PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003889:Desert Climate; D005227:Fatty Acids / Q000737:chemistry; D008835:Micrococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2