title: Association of ALOX5AP with ischemic stroke in eastern Chinese.
abstract: BACKGROUND
  5-lipoxygenase protein (ALOX5AP) has been recognized as a susceptibility gene for stroke and coronary artery diseases. The present study was to explore the role of this gene in the eastern Chinese patients with ischemic stroke.
  
  
  METHODS
  Using a case-control design, we studied 658 patients with ischemic stroke and 704 unrelated population-based controls who were age- and sex-matched. The 658 patients were classified by the Trial of Org 10172 in Acute Stroke Treatment (TOAST). Two single-nucleotide polymorphisms (SNPs) covering ALOX5AP were genotyped.
  
  
  RESULTS
  The genotype frequencies of TG of the SNPs rs17222919 located in the promoter of the ALOX5AP gene were significantly higher in patients with ischemic stroke than in controls (OR (*)=1.34, 95%CI(*)=1.02-1.75), especially in patients with ischemic stroke caused by small-artery occlusion (SAO) (OR (*)=1.40, 95%CI(*)=1.02-1.93). Meanwhile, the genotype frequencies of TG and TG/GG were higher in female patients than in the controls. After specification, the genotype frequencies of TG and TG/GG were higher in the patients than in controls with hypertension. The genotype frequencies of AG and AG/GG of the SNPs rs9579646 located in the intron of the ALOX5AP gene were higher in the controls than in the patients. After specification, the genotype frequencies of TG were higher in the controls than patients without hypertension.
  
  
  CONCLUSIONS
  The present study suggests that sequence variants in the ALOX5AP gene are significantly associated with ischemic stroke.
authors.affiliation: Department of Emergency Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Emergency Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Gan-Nan
authors.initials: GN
authors.lastname: Wang
authors.affiliation: Department of Emergency Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Sun
authors.affiliation: Jiangsu Province Center for Disease Control, Nanjing, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Laboratory of Neurotoxicology, School of Public Health, Nanjing Medical University, Nanjing, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Department of Emergency Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Zhang
keywords: 5-lipoxygenase activating protein (ALOX5AP); Ischemic stroke; Leukotrienes (LTs); Single nucleotide polymorphisms (SNPs); Trial of Org 10172 in Acute Stroke Treatment (TOAST)