title: Effects of Morus alba L. and Natural Products Including Morusin on In Vivo Secretion and In Vitro Production of Airway MUC5AC Mucin.
abstract: BACKGROUND
  It is valuable to find the potential activity of regulating the excessive mucin secretion by the compounds derived from various medicinal plants. We investigated whether aqueous extract of the root bark of Morus alba L. (AMA), kuwanon E, kuwanon G, mulberrofuran G, and morusin significantly affect the secretion and production of airway mucin using in vivo and in vitro experimental models.
  
  
  METHODS
  Effect of AMA was examined on hypersecretion of airway mucin in sulfur dioxide-induced acute bronchitis in rats. Confluent NCI-H292 cells were pretreated with ethanolic extract, kuwanon E, kuwanon G, mulberrofuran G, or morusin for 30 minutes and then stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 hours. The MUC5AC mucin secretion and production were measured by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  AMA stimulated the secretion of airway mucin in sulfur dioxide-induced bronchitis rat model; aqueous extract, ethanolic extract, kuwanon E, kuwanon G, mulberrofuran G and morusin inhibited the production of MUC5AC mucin induced by PMA from NCI-H292 cells, respectively.
  
  
  CONCLUSIONS
  These results suggest that extract of the root bark and the natural products derived from Morus alba L. can regulate the secretion and production of airway mucin and, at least in part, explains the folk use of extract of Morus alba L. as mucoregulators in diverse inflammatory pulmonary diseases.
authors.affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Hyun Jae
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Jiho
authors.initials: J
authors.lastname: Ryu
authors.affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Su Hyun
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju, Korea.
authors.forename: Eun-Rhan
authors.initials: ER
authors.lastname: Woo
authors.affiliation: Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju, Korea.
authors.forename: A Ryun
authors.initials: AR
authors.lastname: Kim
authors.affiliation: Department of Pharmacy, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Korea.
authors.forename: Sang Kook
authors.initials: SK
authors.lastname: Lee
authors.affiliation: Department of Pharmacy, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Korea.
authors.forename: Yeong Shik
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Ju-Ock
authors.initials: JO
authors.lastname: Kim
authors.affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Jang-Hee
authors.initials: JH
authors.lastname: Hong
authors.affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Choong Jae
authors.initials: CJ
authors.lastname: Lee
keywords: Biological Products; Mucins