title: Blood sampling via a peripheral artery catheter decreases cerebral oxygenation index in very low-birthweight infants.
abstract: OBJECTIVE
  This study evaluated the impact of blood sampling via peripheral arterial catheters on cerebral oxygenation and blood volume as a function of blood sampling velocity.
  
  
  METHODS
  Near-infrared spectroscopy was applied to 20 very low-birthweight infants during peripheral arterial blood sampling. Changes in cerebral oxygenated, deoxygenated and total haemoglobin, cerebral blood volume and cerebral oxygenation index were recorded. Heart rate and oxygen saturation were measured continuously. To assess the impact of blood sampling velocity, both fast 40-sec and slow 70-sec sampling procedures were performed in a crossover study design, in which the order of sampling velocities was randomised for each patient.
  
  
  RESULTS
  Both fast and slow blood sampling procedures resulted in a significant decrease in cerebral oxygenation index (fast, p = 0.002, slow, p = 0.008), and an increase in mean heart rate (both p = 0.02) and mean blood pressure (p = 0.02 and 0.04). Oxygenated and total haemoglobin and cerebral blood volume only decreased significantly after slow blood sampling (p < 0.001).
  
  
  CONCLUSIONS
  Blood sampling from peripheral arterial catheters leads to significant fluctuations in cerebral oxygenation independent of the sampling velocity. Changes are comparable to those reported from umbilical blood sampling. We advise that blood sampling should be restricted as much as possible.
authors.affiliation: Department of Neonatology, University of Cologne Children's Hospital, Cologne, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Mehler
authors.forename: Mareike
authors.initials: M
authors.lastname: Nowak
authors.forename: André
authors.initials: A
authors.lastname: Oberthuer
authors.forename: Moritz
authors.initials: M
authors.lastname: Hahn
authors.forename: Bernhard
authors.initials: B
authors.lastname: Roth
authors.forename: Angela
authors.initials: A
authors.lastname: Kribs
mesh_terms: D001800:Blood Specimen Collection / Q000379:methods*; D001810:Blood Volume / Q000502:physiology; D001921:Brain / Q000378:metabolism*; D002406:Catheterization, Peripheral; D002560:Cerebrovascular Circulation / Q000502:physiology; D018592:Cross-Over Studies; D005260:Female; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D007235:Infant, Premature, Diseases / Q000378:metabolism* / Q000503:physiopathology; D019102:Infant, Very Low Birth Weight; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D019265:Spectroscopy, Near-Infrared; D013997:Time Factors
chemical_list: D006454:Hemoglobins; D010100:Oxygen
keywords: Blood sampling velocity; Cerebral haemoglobin oxygenation; Peripheral arterial catheters; Very low-birthweight infant