title: Mechanisms of tomato mosaic virus RNA replication and its inhibition by the host resistance factor Tm-1.
abstract: In the plant immune system, sensor proteins encoded by dominant resistance genes activate a defense response upon pathogen infection. The tomato mosaic virus (ToMV) resistance gene Tm-1 is exceptional in that it inhibits ToMV multiplication without inducing a defense response. Several lines of evidence had suggested that Tm-1 encodes a direct inhibitor of ToMV RNA replication. The Tm-1 gene product was identified by purification of an inhibitor protein using a cell-free translation and replication system for ToMV RNA. Further analyses using the system showed that Tm-1 bound ToMV replication proteins, and that the Tm-1-bound ToMV replication proteins retained the ability to bind membranes, while Tm-1 inhibited replication complex formation on the membranes.
authors.affiliation: Plant-Microbe Interactions Research Unit, Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan. Electronic address: bashi@affrc.go.jp.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ishibashi
authors.affiliation: Plant-Microbe Interactions Research Unit, Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishikawa
mesh_terms: D000998:Antiviral Agents / Q000378:metabolism; D018551:Solanum lycopersicum / Q000276:immunology* / Q000821:virology*; D010940:Plant Proteins / Q000378:metabolism*; D055503:Protein Multimerization; D012367:RNA, Viral / Q000096:biosynthesis*; D012324:RNA-Dependent RNA Polymerase / Q000037:antagonists & inhibitors*; D017898:Tobamovirus / Q000502:physiology*; D014779:Virus Replication
chemical_list: D000998:Antiviral Agents; D010940:Plant Proteins; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase