title: Characterization of natural organic colorants in historical and art objects by high-performance liquid chromatography.
abstract: High-performance liquid chromatography plays an important role in analysis of historical organic colorants. A number of papers have been published in this field over the last 30 years. Classification of the most commonly used natural dyes and an overview of high-performance liquid chromatography methods with main focus on recent works (2008 to the beginning of 2014) are provided. The review deals with an entire analytical protocol covering sample preparation, chromatographic separation, and suitable detection (UV/visible and fluorescent spectroscopy and mass spectrometric techniques). High-performance liquid chromatography has been successfully used in the complete characterization of some organic dyestuffs present in historical and art objects. The possibilities and difficulties for identification of natural sources of historical colorants are also discussed.
authors.affiliation: Faculty of Science, Department of Analytical Chemistry, Regional Centre of Advanced Technologies and Materials, Palacký University in Olomouc, Olomouc, Czech Republic.
authors.forename: Volodymyr
authors.initials: V
authors.lastname: Pauk
authors.forename: Petr
authors.initials: P
authors.lastname: Barták
authors.forename: Karel
authors.initials: K
authors.lastname: Lemr
keywords: Anthraquinoids; Flavonoids; Indigoids; Lake pigments; Tannins