title: Stem cell markers OCT4 and nestin in laryngeal squamous cell carcinoma and their relation to survivin expression.
abstract: We explored the expression of the stem cell markers OCT4 and nestin in laryngeal squamous cell carcinoma (LSCC) and investigated their relationship to survivin expression. Eighty-five LSCC, and 62 non-neoplastic laryngeal tissues were analyzed immunohistochemically for the presence of OCT4, nestin and survivin. Marker expression was correlated to clinicopathological parameters. The positive detection rates of OCT4 (42.35%) and nestin (51.76%) in LSCC were higher than those of non-neoplastic mucosa (p<0.05). OCT4 expression was positively associated with nestin expression (p=0.0001). High expression of both OCT4 and nestin was associated with higher tumor grade (p=0.0001). Also, high OCT4 expression was related to higher T stage (p=0.0001). Co-expression of OCT4 and nestin was more significantly associated with glottic location, higher T stage and nodal metastasis than high expression of either marker (p=0.015, 0.006 and 0.008, respectively). Survivin expression was not significantly related to expression of OCT4 or nestin (p=0.094 and 0.266, respectively). OCT4 and nestin are overexpressed in LSCC and may contribute to laryngeal carcinogenesis. Their co-expression may help to predict the lymph node metastatic potential of LSCC. No relationship was detected between expression of survivin and OCT4 or nestin.
authors.affiliation: Department of Pathology, Faculty of Medicine, University of Alexandria, Alexandria, Egypt. Electronic address: nevineldeeb@yahoo.com.
authors.forename: Nevine M F
authors.initials: NM
authors.lastname: El Deeb
authors.affiliation: Department of Pathology, Faculty of Medicine, University of Alexandria, Alexandria, Egypt. Electronic address: pathologisteman@yahoo.com.
authors.forename: Eman
authors.initials: E
authors.lastname: Abdelzaher
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism*; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D008875:Middle Aged; D064231:Nestin / Q000378:metabolism*; D050814:Octamer Transcription Factor-3 / Q000378:metabolism*; D011379:Prognosis; D013234:Stem Cells / Q000378:metabolism; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D015415:Biomarkers; D051034:Inhibitor of Apoptosis Proteins; C577484:NES protein, human; D064231:Nestin; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D000077022:Survivin
keywords: Laryngeal carcinoma; Nestin; OCT4; Survivin