title: Multiplexed miRNA fluorescence in situ hybridization for formalin-fixed paraffin-embedded tissues.
abstract: Multiplexed miRNA fluorescence in situ hybridization (miRNA FISH) is an advanced method for visualizing differentially expressed miRNAs, together with other reference RNAs, in archival tissues. Some miRNAs are excellent disease biomarkers due to their abundance and cell-type specificity. However, these short RNA molecules are difficult to visualize due to loss by diffusion, probe mishybridization, and signal detection and signal amplification issues. Here, we describe a reliable and adjustable method for visualizing and normalizing miRNA signals in formalin-fixed paraffin-embedded (FFPE) tissue sections.
authors.affiliation: Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, 10065, USA.
authors.forename: Neil
authors.initials: N
authors.lastname: Renwick
authors.forename: Pavol
authors.initials: P
authors.lastname: Cekan
authors.forename: Claudia
authors.initials: C
authors.lastname: Bognanni
authors.forename: Thomas
authors.initials: T
authors.lastname: Tuschl
mesh_terms: D005456:Fluorescent Dyes / Q000032:analysis; D005557:Formaldehyde / Q000737:chemistry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D035683:MicroRNAs / Q000032:analysis*; D008856:Microscopy, Fluorescence / Q000379:methods; D016612:Paraffin Embedding / Q000379:methods; D015347:RNA Probes / Q000032:analysis; D016707:Tissue Fixation / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D035683:MicroRNAs; D015347:RNA Probes; D005557:Formaldehyde