title: Structures of naturally evolved CUP1 tandem arrays in yeast indicate that these arrays are generated by unequal nonhomologous recombination.
abstract: An important issue in genome evolution is the mechanism by which tandem duplications are generated from single-copy genes. In the yeast Saccharomyces cerevisiae, most strains contain tandemly duplicated copies of CUP1, a gene that encodes a copper-binding metallothionein. By screening 101 natural isolates of S. cerevisiae, we identified five different types of CUP1-containing repeats, as well as strains that only had one copy of CUP1. A comparison of the DNA sequences of these strains indicates that the CUP1 tandem arrays were generated by unequal nonhomologous recombination events from strains that had one CUP1 gene.
authors.affiliation: Department of Molecular Genetics and Microbiology and University Program in Genetics and Genomics, Duke University Medical Center, Durham, North Carolina 27710.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Molecular Genetics and Microbiology and University Program in Genetics and Genomics, Duke University Medical Center, Durham, North Carolina 27710.
authors.forename: Pooja K
authors.initials: PK
authors.lastname: Strope
authors.affiliation: Department of Molecular Genetics and Microbiology and University Program in Genetics and Genomics, Duke University Medical Center, Durham, North Carolina 27710.
authors.forename: Stanislav G
authors.initials: SG
authors.lastname: Kozmin
authors.affiliation: Department of Molecular Genetics and Microbiology and University Program in Genetics and Genomics, Duke University Medical Center, Durham, North Carolina 27710.
authors.forename: John H
authors.initials: JH
authors.lastname: McCusker
authors.affiliation: Department of Molecular Genetics and Microbiology and University Program in Genetics and Genomics, Duke University Medical Center, Durham, North Carolina 27710.
authors.forename: Fred S
authors.initials: FS
authors.lastname: Dietrich
authors.affiliation: Physical Sciences Directorate, U. S. Army Research Office, Research Triangle Park, North Carolina 27709.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Kokoska
authors.affiliation: Department of Molecular Genetics and Microbiology and University Program in Genetics and Genomics, Duke University Medical Center, Durham, North Carolina 27710 tom.petes@duke.edu.
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Petes
mesh_terms: D019143:Evolution, Molecular; D020440:Gene Duplication; D059765:Homologous Recombination; D008668:Metallothionein / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: C500769:CUP1-1 protein, S cerevisiae; D008668:Metallothionein
keywords: Saccharomyces cerevisiae; copper resistance; recombination; tandemly repeated genes