title: Conversion of the Pseudomonas aeruginosa Quinolone Signal and Related Alkylhydroxyquinolines by Rhodococcus sp. Strain BG43.
abstract: A bacterial strain, which based on the sequences of its 16S rRNA, gyrB, catA, and qsdA genes, was identified as a Rhodococcus sp. closely related to Rhodococcus erythropolis, was isolated from soil by enrichment on the Pseudomonas quinolone signal [PQS; 2-heptyl-3-hydroxy-4(1H)-quinolone], a quorum sensing signal employed by the opportunistic pathogen Pseudomonas aeruginosa. The isolate, termed Rhodococcus sp. strain BG43, cometabolically degraded PQS and its biosynthetic precursor 2-heptyl-4(1H)-quinolone (HHQ) to anthranilic acid. HHQ degradation was accompanied by transient formation of PQS, and HHQ hydroxylation by cell extracts required NADH, indicating that strain BG43 has a HHQ monooxygenase isofunctional to the biosynthetic enzyme PqsH of P. aeruginosa. The enzymes catalyzing HHQ hydroxylation and PQS degradation were inducible by PQS, suggesting a specific pathway. Remarkably, Rhodococcus sp. BG43 is also capable of transforming 2-heptyl-4-hydroxyquinoline-N-oxide to PQS. It thus converts an antibacterial secondary metabolite of P. aeruginosa to a quorum sensing signal molecule.
authors.affiliation: Institute of Molecular Microbiology and Biotechnology, University of Münster, Münster, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Müller
authors.affiliation: Institute of Molecular Microbiology and Biotechnology, University of Münster, Münster, Germany.
authors.forename: Franziska S
authors.initials: FS
authors.lastname: Birmes
authors.affiliation: Institute of Molecular Microbiology and Biotechnology, University of Münster, Münster, Germany.
authors.forename: Heiko
authors.initials: H
authors.lastname: Niewerth
authors.affiliation: Institute of Molecular Microbiology and Biotechnology, University of Münster, Münster, Germany fetzner@uni-muenster.de.
authors.forename: Susanne
authors.initials: S
authors.lastname: Fetzner
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001711:Biotransformation; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D015363:Quinolones / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: C407944:2-heptyl-3-hydroxy-4-quinolone; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D015363:Quinolones; D012336:RNA, Ribosomal, 16S