title: Extended metAFLP approach in studies of tissue culture induced variation (TCIV) in triticale.
abstract: We present the development of the theoretical background of the metAFLP approach which allows for partition of complex variation into sequence changes, de novo methylation and demethylation of the regenerants derived via in vitro tissue culture methods in the case of triticale. It was demonstrated that, independent of whether andro- or embryogenesis was used for plant regeneration, the level of sequence changes identified between regenerants is about 10 %. Moreover, DNA demethylation prevails over de novo methylation of the regenerants compared to the donor plant. The metAFLP approach allows for the evaluation of numerous quantitative characteristics. For instance, one may quantify the number of sites unaffected by tissue culture approaches, global site DNA methylation etc. It is suggested that the approach could be useful for breeders in order to control plant material uniformity or for the evaluation of modified in vitro tissue culture approaches allowing for control of the (epi)mutation level. The extended metAFLP approach presented here delivers sufficient background for the evaluation of software that could facilitate analyses of the tissue culture induced variation.
authors.affiliation: Department of Plant Physiology and Biochemistry, Plant Breeding and Acclimatization Institute, National Research Institute, 05-870 Błonie, Radzików Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Machczyńska
authors.affiliation: Department of Plant Physiology and Biochemistry, Plant Breeding and Acclimatization Institute, National Research Institute, 05-870 Błonie, Radzików Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Orłowska
authors.affiliation: Department of Plant Biotechnology and Cytogenetics, Plant Breeding and Acclimatization Institute, National Research Institute, 05-870 Błonie, Radzików Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Zimny
authors.affiliation: Department of Plant Physiology and Biochemistry, Plant Breeding and Acclimatization Institute, National Research Institute, 05-870 Błonie, Radzików Poland.
authors.forename: Piotr Tomasz
authors.initials: PT
authors.lastname: Bednarek
keywords: (Epi)genetics; Extended metAFLP; Tissue culture induced variation; Triticale