title: Mouse colorectal cancer an early detection approach using nonlinear microscopy.
abstract: Murine induced colon cancer has been used to demonstrate that Second Harmonic Generation (SHG) microscopy images, combined with Two-Photon Excitation Fluorescence (TPEF) and specific quantization scoring methods allow distinguishing early alterations in colon mucosa. TPEF was used only to identified crypts and submucosa regions, whereas the image analysis was used to get quantitative data (Integrated Intensity and Aspect Ratio scoring) of different cancer stages. The submucosa amount of collagen fibers was significant and their orientation suffering proportional changes with the development of the pathological processes. Both after the fourth and eighth weeks after colon cancer induction, integrated intensity and aspect ratio values have shown significant statistical differences compared with control samples. Thus, SHG microscopy has proved to be a useful quantitative tool to highlight early changes of submucosa and the progression of these through the cancer development.
authors.affiliation: Microscopy Laboratory Applied to Molecular and Cellular Studies, Engineering School, National University of Entre Rios, (3101) Oro Verde, Entre Rios, Argentina.
authors.forename: Mariana
authors.initials: M
authors.lastname: Bianchi
authors.affiliation: Microscopy Laboratory Applied to Molecular and Cellular Studies, Engineering School, National University of Entre Rios, (3101) Oro Verde, Entre Rios, Argentina Research and Transfer Center of Entre Ríos (CITER) CONICET-UNER (3101) Oro Verde. Entre Ríos. Argentina.
authors.forename: Javier
authors.initials: J
authors.lastname: Adur
authors.affiliation: Microscopy Laboratory Applied to Molecular and Cellular Studies, Engineering School, National University of Entre Rios, (3101) Oro Verde, Entre Rios, Argentina.
authors.forename: Silvana Y
authors.initials: SY
authors.lastname: Ruff
authors.affiliation: Microscopy Laboratory Applied to Molecular and Cellular Studies, Engineering School, National University of Entre Rios, (3101) Oro Verde, Entre Rios, Argentina.
authors.forename: María F
authors.initials: MF
authors.lastname: Izaguirre
authors.affiliation: INFABiC - National Institute of Science and Technology on Photonics Applied to Cell Biology, Campinas, São Paulo, Brazil.
authors.forename: Hernandes F
authors.initials: HF
authors.lastname: Carvalho
authors.affiliation: INFABiC - National Institute of Science and Technology on Photonics Applied to Cell Biology, Campinas, São Paulo, Brazil.
authors.forename: Carlos L
authors.initials: CL
authors.lastname: Cesar
authors.affiliation: Microscopy Laboratory Applied to Molecular and Cellular Studies, Engineering School, National University of Entre Rios, (3101) Oro Verde, Entre Rios, Argentina.
authors.forename: Víctor H
authors.initials: VH
authors.lastname: Casco
mesh_terms: D000465:Algorithms; D000818:Animals; D015179:Colorectal Neoplasms / Q000473:pathology*; D055088:Early Detection of Cancer / Q000379:methods*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D064847:Multimodal Imaging / Q000379:methods*; D017711:Nonlinear Dynamics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
keywords: Colon cancer; early detection; second harmonic generation; two-photon excitation fluorescence