title: Effects of specific organs on seed oil accumulation in Brassica napus L.
abstract: Seed oil content is an important agricultural characteristic in rapeseed breeding. Genetic analysis shows that the mother plant and the embryo play critical roles in regulating seed oil accumulation. However, the overwhelming majority of previous studies have focused on oil synthesis in the developing seed of rapeseed. In this study, to elucidate the roles of reproductive organs on oil accumulation, silique, ovule, and embryo from three rapeseed lines with high oil content (zy036, 6F313, and 61616) were cultured in vitro. The results suggest that zy036 silique wall, 6F313 seed coat, and 61616 embryo have positive impacts on the seed oil accumulation. In zy036, our previous studies show that high photosynthetic activity of the silique wall contributes to seed oil accumulation (Hua et al., 2012). Herein, by transcriptome sequencing and sucrose detection, we found that sugar transport in 6F313 seed coat might regulate the efficiency of oil synthesis by controlling sugar concentration in ovules. In 61616 embryos, high oil accumulation efficiency was partly induced by the elevated expression of fatty-acid biosynthesis-related genes. Our investigations show three organ-specific mechanisms regulating oil synthesis in rapeseed. This study provides new insights into the factors affecting seed oil accumulation in rapeseed and other oil crops.
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hua
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, People's Republic of China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Guo
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, People's Republic of China.
authors.forename: Xingchao
authors.initials: X
authors.lastname: Sun
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, People's Republic of China.
authors.forename: Xinfa
authors.initials: X
authors.lastname: Wang
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, People's Republic of China.
authors.forename: Guihua
authors.initials: G
authors.lastname: Liu
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, People's Republic of China. Electronic address: wanghz@oilcrops.cn.
authors.forename: Hanzhong
authors.initials: H
authors.lastname: Wang
mesh_terms: D001692:Biological Transport; D029688:Brassica napus / Q000235:genetics / Q000378:metabolism*; D029741:Brassica rapa; D005227:Fatty Acids / Q000096:biosynthesis* / Q000235:genetics; D005638:Fruit / Q000378:metabolism / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D056771:Ovule / Q000378:metabolism*; D010788:Photosynthesis; D010938:Plant Oils / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism; D059467:Transcriptome
chemical_list: D005227:Fatty Acids; D010938:Plant Oils; D013395:Sucrose
keywords: Maternal effect; Oil content; Oil regulation; Organ; Rapeseed