title: Basophil activation tests: time for a reconsideration.
abstract: Challenges in in vitro allergy diagnostics lie in the development of accessible and reliable assays allowing identification of all offending allergens and cross-reactive structures. Flow-assisted analysis and quantification of in vitro activated basophils serves as a diagnostic instrument with increasing applications developed over the years. From the earliest days it was clear that the test could constitute a diagnostic asset in basophil-mediated hypersensitivity. However, utility of the basophil activation test should be reassessed regarding difficulties with preparation, characterization and validation of allergen extracts; availability and the potential of more accessible diagnostics. Today, the added value mainly lies in diagnosis of immediate drug hypersensitivity. Other potential indications are monitoring venom-immunotherapy and follow-up of natural history of food allergies. However, results in these nondiagnostic applications are preliminary. We review the most relevant clinical applications of the basophil activation test. Some personal comments and views about perspectives and challenges about flow-assisted allergy diagnosis are made.
authors.affiliation: Department of Immunology - Allergology - Rheumatology, Faculty of Medicine and Health Science, University of Antwerp, Antwerp University Hospital, CDE T.595, Universiteitsplein 1, 2610 Antwerpen, Belgium.
authors.forename: Astrid P
authors.initials: AP
authors.lastname: Uyttebroek
authors.forename: Vito
authors.initials: V
authors.lastname: Sabato
authors.forename: Margaretha A
authors.initials: MA
authors.lastname: Faber
authors.forename: Nathalie
authors.initials: N
authors.lastname: Cop
authors.forename: Chris H
authors.initials: CH
authors.lastname: Bridts
authors.forename: Hilde
authors.initials: H
authors.lastname: Lapeere
authors.forename: Luc S
authors.initials: LS
authors.lastname: De Clerck
authors.forename: Didier G
authors.initials: DG
authors.lastname: Ebo
mesh_terms: D015549:Basophil Degranulation Test; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis*
keywords: CD63; allergy; basophil activation test; diagnosis; drug hypersensitivity; flow-cytometry; food allergy; hymenoptera venom