title: Expression and subcellular localization of metastasis-associated protein 1, its short form, and estrogen receptors in rat placenta.
abstract: Metastasis-associated protein 1 (MTA1) and its short form (MTA1s) regulate the function of estrogen receptors (ERs). Estrogens, via ERs, affect placental growth and fetal development, a process that may involve MTA1 signaling. Expression of MTA1, MTA1s, ERα, and ERβ genes and proteins in rat placentas was studied on 16, 19, and 21 days of gestation (dg). The ERβ messenger RNA decreased significantly toward the end of gestation, whereas its protein level increased in the nuclear fraction on 21 dg. Both MTA1 and MTA1s increased with gestation. Decidual, trophoblast giant, glycogen, and villous trophoblast cells expressed MTA1, ERα, and ERβ proteins on all dg with colocalization of MTA1 with ERα and ERβ in the nucleus and cytoplasm. Expression of MTA1 suggests a possible role in regulating placental functions; considering the repressive function of MTA1 on ERs, the expression of MTA1 suggests that placental cells may be less sensitive to estrogens during late pregnancy.
authors.affiliation: Department of Physiology, Faculty of Medicine, Kuwait University, Kuwait albader@hsc.edu.kw.
authors.forename: Maie Dawoud
authors.initials: MD
authors.lastname: Al-Bader
authors.affiliation: Department of Anatomy, Faculty of Medicine, Kuwait University, Kuwait.
authors.forename: Narayana
authors.initials: N
authors.lastname: Kilarkaje
authors.affiliation: Department of Physiology, Faculty of Medicine, Kuwait University, Kuwait.
authors.forename: Aseel
authors.initials: A
authors.lastname: El-Farra
authors.affiliation: Department of Pathology, Faculty of Medicine, Kuwait University, Kuwait.
authors.forename: Abeer Adnan
authors.initials: AA
authors.lastname: Al-Abdallah
mesh_terms: D000818:Animals; D004958:Estradiol / Q000097:blood; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism*; D047629:Estrogen Receptor beta / Q000235:genetics / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D010920:Placenta / Q000166:cytology / Q000378:metabolism; D011247:Pregnancy; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; C121683:Mta1 protein, rat; D011506:Proteins; D012333:RNA, Messenger; D004958:Estradiol
keywords: MTA1; estrogen receptor alpha; estrogen receptor beta; estrogens; placental growth