title: Bacterial contamination of blood components: Norwegian strategies in identifying donors with higher risk of inducing septic transfusion reactions in recipients.
abstract: Bacterial contamination of blood and its cellular components remains the most common microbiological cause of transfusion associated morbidity and mortality, even in developed countries. This yet unresolved complication is seen more often in platelet transfusions, as platelet concentrates are stored at room temperature, in gas permeable containers with constant agitation, which support bacterial proliferation from relatively low undetectable levels, at the beginning of storage time, to relatively high virulent bacteria titers and endotoxin generation, at the end of shelf life. Accordingly, several combined strategies are introduced and implemented to at least reduce the potential risk of bacterial contaminated products for transfusion. These embody: improved donors arms cleaning; bacterial avoidance by diversion of the first portion of collection; reducing bacterial growth through development of newer storage media for longer platelet shelf life; bacterial load reduction by leucoreduction/viral inactivation, in some countries and eliminating the use potentially contaminated units through screening, through current available testing procedures, though none are not yet fully secure. We have not seen the same reduction in bacterial associated transfusion infections as we have observed for the sharp drop in transfusion associated transmission rates of HIV and hepatitis B and C. This great viral reduction is not only caused by the introduction of newer and more sensitive and specific detection methods for different viruses, but also the identification of donor risk groups through questionnaires and personal interviews. While search for more efficient methods for identifying potential blood donors with asymptomatic bacteremia, as well as a better way for detecting bacteria in stored blood components will be continuing, it is necessary to establish more standardized guidelines for the recognition the adverse reactions in recipients of potentially contaminated units. Efforts also should be also directed to identify blood donors with significant risk of bacteremia, at the time of donation in the first place as a high priority. The goal of this review is to highlights strategies for identifying both the sources of bacterial contamination of blood components in Norway and identifying donors with a higher risk of bacteremia at the time of donation. The key to achieving this goal is initiating continual revising and upgrading the Norwegian transfusion guidelines, based on the transfusion legislation and by introducing a relevant specialized donor bacterial questionnaire.
authors.affiliation: Department of Immunology and Transfusion Medicine, Haukeland University Hospital, Bergen, Norway; Insitute of Clinical Science, Faculty of Medicine and Dentistry, University of Bergen, Bergen, Norway.
authors.forename: Sofie Strand
authors.initials: SS
authors.lastname: Klausen
authors.affiliation: Department of Immunology and Transfusion Medicine, Haukeland University Hospital, Bergen, Norway; Insitute of Clinical Science, Faculty of Medicine and Dentistry, University of Bergen, Bergen, Norway. Electronic address: tor.hervig@helse-bergen.no.
authors.forename: Tor
authors.initials: T
authors.lastname: Hervig
authors.affiliation: International consultancy in Blood Components Quality/Safety and DDR Strategy, London, UK.
authors.forename: Jerard
authors.initials: J
authors.lastname: Seghatchian
authors.affiliation: Insitute of Clinical Science, Faculty of Medicine and Dentistry, University of Bergen, Bergen, Norway; Department of Medicine, Haukeland University Hospital, Bergen, Norway.
authors.forename: Håkon
authors.initials: H
authors.lastname: Reikvam
mesh_terms: D001782:Blood Donors; D001793:Blood Preservation; D058731:Blood Safety / Q000379:methods*; D046148:Donor Selection / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D009664:Norway / Q000453:epidemiology; D017713:Platelet Transfusion / Q000009:adverse effects*; D012307:Risk Factors; D018805:Sepsis / Q000097:blood / Q000453:epidemiology / Q000209:etiology / Q000517:prevention & control*