title: NiO nanoparticles modified with 5,10,15,20-tetrakis(4-carboxyl pheyl)-porphyrin: promising peroxidase mimetics for H2O2 and glucose detection.
abstract: NiO nanoparticles (NPs) and 5,10,15,20-tetrakis(4-carboxyl pheyl)-porphyrin (H2TCPP) functionalized NiO nanoparticles (H2TCPP-NiO nanocomposites) have been prepared by a facile method and characterized by powder X-ray diffraction (XRD), transmission electron microscope (TEM) and Fourier transform infrared spectra (FTIR), respectively. NiO NPs and H2TCPP-NiO nanocomposites have been proven to function as peroxidase mimetics that can catalyze the reaction of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2 to produce a blue color reaction. Kinetic analysis indicated that the catalytic behavior was in accord with typical Michaelis-Menten kinetics. And these nanoparticles also exhibited strong affinity for the substrates of 3,3,5,5-tetramethylbiphenyl dihydrochloride (TMB). Experimental results showed that H2TCPP-NiO NPs exhibited a high sensitivity and a low detection limit towards H2O2 (8.0 × 10(-6) M). The H2TCPP-NiO NPs/glucose oxidase (GOx)/TMB system provides a novel colorimetric sensor for glucose and shows good response toward glucose detection over arrange of 0.05-0.50 mM with a limit of detection 2.0 × 10(-5)M. Fluorescence probe experiments demonstrated that the peroxidase-like activity of H2TCPP-NiO NPs originated from the generation of OH radical. Thus it may provide great potential applications in biomedicine, biotechnology and environmental chemistry.
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China. Electronic address: qyliu@sdust.edu.cn.
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Liu
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Yanting
authors.initials: Y
authors.lastname: Yang
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Renren
authors.initials: R
authors.lastname: Zhu
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Shao
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Shanguang
authors.initials: S
authors.lastname: Yang
authors.affiliation: School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Xu
mesh_terms: D000327:Adsorption; D040761:Biomimetic Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D002384:Catalysis; D003124:Colorimetry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009532:Nickel / Q000737:chemistry*; D009195:Peroxidase / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry*
chemical_list: D011166:Porphyrins; C527224:meso-tetra(4-nido-carboranylphenyl)porphyrin; D009532:Nickel; D006861:Hydrogen Peroxide; C028007:nickel monoxide; D009195:Peroxidase; D005947:Glucose
keywords: Colorimetric; Glucose; Nanocomposite; NiO; Peroxidase; Porphyrin