title: Compartmentation and localization of bioactive metabolites in different organs of Allium roylei.
abstract: To investigate the involvement of Allium roylei metabolites in the plant's defenses, a comprehensive analysis of the content of cysteine sulfoxides, flavonols, polyphenols, ascorbic acid, and saponins was carried out in the various organs of this species. Metabolomics high performance liquid chromatography (HPLC), spectral-based analysis, and histochemcial studies have given important insight to the validity of saponins as a key component involved in plant protection. The root-basal stem, bulb, and leaf extracts exhibited 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity with inhibition concentration (IC(50)) ranging from 0.649 to 0.757 mg/mL. The antimicrobial properties of the saponin and flavonoid crude extracts were evaluated. The saponin extracts demonstrated significant antifungal activity depending on the applied concentration, and the growth inhibition rate of the tested fungal pathogens ranged from 1.07 to 47.76%. No appreciable antibacterial activity was recorded in the same sample.
authors.affiliation: a The United Graduate School of Agricultural Sciences , Tottori University , Tottori , Japan.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Abdelrahman
authors.forename: Sho
authors.initials: S
authors.lastname: Hirata
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Ito
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamauchi
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Shigyo
mesh_terms: D000490:Allium / Q000378:metabolism*; D000890:Anti-Infective Agents / Q000378:metabolism / Q000494:pharmacology; D001205:Ascorbic Acid / Q000378:metabolism; D001692:Biological Transport; D005419:Flavonoids / Q000378:metabolism / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000378:metabolism / Q000494:pharmacology; D009928:Organ Specificity; D059808:Polyphenols / Q000378:metabolism; D012503:Saponins / Q000378:metabolism / Q000494:pharmacology; D013454:Sulfoxides / Q000378:metabolism
chemical_list: D000890:Anti-Infective Agents; D005419:Flavonoids; D016166:Free Radical Scavengers; D059808:Polyphenols; D012503:Saponins; D013454:Sulfoxides; D001205:Ascorbic Acid
keywords: antimicrobial activity; antioxidant activity; cysteine sulfoxides; flavonoids; saponins