title: Bioanalytical methods for the metalloproteomics study of bovine longissimus thoracis muscle tissue with different grades of meat tenderness in the Nellore breed (Bos indicus).
abstract: The work describes a metalloproteomics study of bovine muscle tissue with different grades of meat tenderness from animals of the Nellore breed (Bos indicus) based on protein separation by two-dimensional gel electrophoresis, the identification of calcium ions in protein spots by X-ray fluorescence (SR-XRF) and the characterisation of proteins by electrospray ionisation mass spectrometry. Forty (40) specimens were selected and divided into two experimental groups: animals with tough meat (TO) and animals with tender meat (TE). A third group (P) of Piedmontese breed animals (Bos taurus) was included to serve as a comparative model for the level of meat tenderness. The procedures were efficient and preserved the metal-protein structure, enabling calcium detection in protein spots by SR-XRF at a given molecular weight range of 14-97kDa. Two proteins (pyruvate kinase and albumin) were inferred to be related to the phenotypical differences in animals from the different groups.
authors.affiliation: Department of Animal Breeding and Nutrition, College of Veterinary and Animal Science, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
authors.forename: Welder Angelo
authors.initials: WA
authors.lastname: Baldassini
authors.affiliation: Institute of Biosciences, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil. Electronic address: braga_ca@yahoo.com.br.
authors.forename: Camila Pereira
authors.initials: CP
authors.lastname: Braga
authors.affiliation: Department of Animal Breeding and Nutrition, College of Veterinary and Animal Science, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
authors.forename: Luis Artur Loyola
authors.initials: LA
authors.lastname: Chardulo
authors.affiliation: Department of Animal Breeding and Nutrition, College of Veterinary and Animal Science, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
authors.forename: Josineudson Augusto
authors.initials: JA
authors.lastname: Vasconcelos Silva
authors.affiliation: Department of Animal Science, College of Agriculture and Veterinary Science, São Paulo State University (UNESP), Jaboticabal, São Paulo, Brazil.
authors.forename: Jessica Moraes
authors.initials: JM
authors.lastname: Malheiros
authors.affiliation: Department of Animal Science, College of Agriculture and Veterinary Science, São Paulo State University (UNESP), Jaboticabal, São Paulo, Brazil.
authors.forename: Lúcia Galvão
authors.initials: LG
authors.lastname: de Albuquerque
authors.affiliation: Institute of Biosciences, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
authors.forename: Talita Tanaka
authors.initials: TT
authors.lastname: Fernandes
authors.affiliation: Institute of Biosciences, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
authors.forename: Pedro de Magalhães
authors.initials: Pde M
authors.lastname: Padilha
mesh_terms: D000818:Animals; D001947:Breeding; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D008460:Meat / Q000032:analysis*; D064170:Paraspinal Muscles / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*
chemical_list: D011506:Proteins
keywords: 2D-PAGE; Beef cattle; Calcium; Mass spectrometry; Zebu genotype