title: Thinning of dermas with the increasing age may be against by tetrahydroxystilbene glucoside in mice.
abstract: OBJECTIVE
  To research and reveal Tetrahydroxystilbene glucoside (TSG) anti-thinning effect of dermas on skin of ageing mice.
  
  
  METHODS
  The dermal layer thickness was measured with hematoxylin-eosin (HE) staining; the levels of collagen and elastic fibers were measured with immunohistochemical staining; the levels of insulin, insulin-like growth factor-1 (IGF-1), and two receptors of insulin and IGF-1 were measured with Elisa kits; the levels of Ca(2+) and P were measured with ELISA kits.
  
  
  RESULTS
  TSG and Polygonum multiflorum extract (PME) made thicken dermal layer thickness (P<0.01, vs. control group); promoted collagen fiber expression (P<0.01, vs. control group, 22.94% and 28.26% vs. 20.41%); reduced the levels of insulin (P<0.01, vs. control group, 2.50 ng/ml and 2.69 ng/ml vs. 3.04 ng/ml), insulin like growth factor 1 (IGF-1, P<0.01, vs. control group, 154.75 ng/ml and 155.60 ng/ml vs. 209.28 ng/ml), receptors of insulin (P<0.01, vs. control group, 0.423 ng/ml and 0.426 ng/ml vs. 0.648 ng/ml) and IGF-1 (P<0.01, vs. control group, 71.96 ng/ml and 81.68 ng/ml vs. 87.02 ng/ml) in aging mice skin; raised the levels of Ca(2+) and P in serum in mice (P<0.01, vs. control group, 1.24 mol/ml and 1.30 mol/ml vs. 1.08 mol/ml; P<0.01, vs. control group, 2.00 mol/ml and 2.03 mol/ml vs. 1.197 mol/ml).
  
  
  CONCLUSIONS
  TSG and PME showed their protections to skin aging in mice challenged with control groups. It ensured the anti-thinning effect of dermas of TSG and provided two potential factors (insulin/IGF-1 signal pathway and the level of Ca(2+)) related to skin senescence of aging mice.
authors.affiliation: Department of Natural Medicine, School of Pharmacy, Fourth Military Medical University 169 West Changle Road, Xi'an 710032, China ; The Cultivation Project of Collaborative Innovation Center for Chinese Medicine in QinBa Mountains Xi'an 710032, China.
authors.forename: Xuanxuan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Dermatology, Southwest Hospital, Third Military Medical University Chongqing 400038, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Ge
authors.affiliation: Department of Natural Medicine, School of Pharmacy, Fourth Military Medical University 169 West Changle Road, Xi'an 710032, China ; The Cultivation Project of Collaborative Innovation Center for Chinese Medicine in QinBa Mountains Xi'an 710032, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Department of Natural Medicine, School of Pharmacy, Fourth Military Medical University 169 West Changle Road, Xi'an 710032, China ; The Cultivation Project of Collaborative Innovation Center for Chinese Medicine in QinBa Mountains Xi'an 710032, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Natural Medicine, School of Pharmacy, Fourth Military Medical University 169 West Changle Road, Xi'an 710032, China ; The Cultivation Project of Collaborative Innovation Center for Chinese Medicine in QinBa Mountains Xi'an 710032, China.
authors.forename: Jiyuan
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Natural Medicine, School of Pharmacy, Fourth Military Medical University 169 West Changle Road, Xi'an 710032, China ; The Cultivation Project of Collaborative Innovation Center for Chinese Medicine in QinBa Mountains Xi'an 710032, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.affiliation: Department of Natural Medicine, School of Pharmacy, Fourth Military Medical University 169 West Changle Road, Xi'an 710032, China ; The Cultivation Project of Collaborative Innovation Center for Chinese Medicine in QinBa Mountains Xi'an 710032, China.
authors.forename: Siwang
authors.initials: S
authors.lastname: Wang
keywords: Ca2+; Tetrahydroxystilbene glucoside; collagen fiber; dermal layer thickness; insulin/IGF-1 signal pathway