title: Serological proteome analysis of Corynebacterium pseudotuberculosis isolated from different hosts reveals novel candidates for prophylactics to control caseous lymphadenitis.
abstract: Caseous lymphadenitis (CLA) is a highly prevalent disease in goats and sheep worldwide, which is caused by Corynebacterium pseudotuberculosis. Although several prophylactic methods against CLA have been proposed previously, the identification of new C. pseudotuberculosis proteins that are really produced during the infectious process is still needed to improve efficiency and accuracy in vaccines and diagnostics. In this study, we used optimized conditions for serological proteome analysis (SERPA) in order to identify new immune-reactive proteins in C. pseudotuberculosis culture supernatants of two strains, 1002 and C231, isolated from goats and sheep, respectively. Using a sheep and goat serum pool, 13 novel immune-reactive exoproteins common to the two strains were identified. Four of these proteins present known functions and were already described as immune-reactive proteins in other microorganisms, whereas the other nine are of unknown function and show low similarity with proteins from other bacterial species. These data reveal promising targets for immunoprophylactic methods against CLA.
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; Institut National de la Recherche Agronomique, UMR1253, Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France; AGROCAMPUS OUEST, UMR1253, Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France.
authors.forename: Nubia
authors.initials: N
authors.lastname: Seyffert
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Renata Faria
authors.initials: RF
authors.lastname: Silva
authors.affiliation: Institut National de la Recherche Agronomique, UMR1253, Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France; AGROCAMPUS OUEST, UMR1253, Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Jardin
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; Institut National de la Recherche Agronomique, UMR1253, Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France; AGROCAMPUS OUEST, UMR1253, Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France.
authors.forename: Wanderson Marques
authors.initials: WM
authors.lastname: Silva
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Thiago Luiz de Paula
authors.initials: TL
authors.lastname: Castro
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Natayme Rocha
authors.initials: NR
authors.lastname: Tartaglia
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Karina Talita de Oliveira
authors.initials: KT
authors.lastname: Santana
authors.affiliation: Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Brazil.
authors.forename: Ricardo Wagner
authors.initials: RW
authors.lastname: Portela
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Brazil.
authors.forename: Artur
authors.initials: A
authors.lastname: Silva
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Anderson
authors.initials: A
authors.lastname: Miyoshi
authors.affiliation: Institut National de la Recherche Agronomique, UMR1253, Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France; AGROCAMPUS OUEST, UMR1253, Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Le Loir
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. Electronic address: vasco@icb.ufmg.br.
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000627:therapeutic use*; D003354:Corynebacterium Infections / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control / Q000662:veterinary*; D016925:Corynebacterium pseudotuberculosis / Q000235:genetics / Q000378:metabolism*; D055808:Drug Discovery / Q000379:methods; D015511:Goat Diseases / Q000382:microbiology*; D006041:Goats; D008199:Lymphadenitis / Q000517:prevention & control / Q000662:veterinary*; D040901:Proteomics; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D034561:Sheep, Domestic
chemical_list: D001426:Bacterial Proteins
keywords: Caseous lymphadenitis; Corynebacterium pseudotuberculosis; Seroproteome; Small ruminants