title: Functional expression of recombinant human macrophage β-glucan receptor dectin-1 using baculovirus-silkworm expression system.
abstract: Human macrophage dectin-1, a type II transmembrane β-glucan receptor, was expressed as a fusion protein with an N-terminal hexahistidine tag in a baculovirus-silkworm expression system and assayed for binding activity. Recombinant dectin-1 specifically bound to some β-glucans, and the neck domain and N-linked oligosaccharide chains of human dectin-1 did not affect the ligand binding activity and specificity of the receptor.
authors.affiliation: a Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agriculture , Meijo University, Tempaku-ku , Nagoya 468-8502 , Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Ujita
authors.forename: Shota
authors.initials: S
authors.lastname: Koike
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Yamauchi
authors.forename: Noburo
authors.initials: N
authors.lastname: Kiochi
authors.forename: Hiroki
authors.initials: H
authors.lastname: Yura
authors.forename: Mayumi
authors.initials: M
authors.lastname: Yamanaka
authors.forename: Hiroki
authors.initials: H
authors.lastname: Okumura
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D012831:Bombyx / Q000235:genetics*; D002460:Cell Line; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D037181:Lectins, C-Type / Q000235:genetics* / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D047071:beta-Glucans / Q000378:metabolism*; D000099181:Dectin-1
chemical_list: D037181:Lectins, C-Type; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins; D047071:beta-Glucans; D000099181:Dectin-1
keywords: N-linked oligosaccharide chains; baculovirus–silkworm expression system; human dectin-1; neck domain; β-glucan receptor