title: PPZPMs--a novel group of cyclic lipodepsipeptides produced by the Phytophthora alni associated strain Pseudomonas sp. JX090307--the missing link between the viscosin and amphisin group.
abstract: The closely related to the Pseudomonas orientalis strain Pseudomonas sp. acc. no. JX090307 was isolated from hyphae of the phytopathogenic oomycete Phytophthora alni spp. alni. In in-vitro antagonistic tests, the living bacterium JX090307 and its cell extract showed antibiosis activity against different fungal pathogens of forest tree species, particularly against Verticillium dahliae and some strains of P. alni ssp. alni. Investigating the cell extract of JX090307 by means of LC-ESI-Q-TOF-MS and -MS/MS techniques, more than 30 cyclic lipodepsipeptids (CLPs) were found. 24 of them belong to a novel group of CLPs named PPZPM. The cyclic lipodepsidecapeptides PPZPMs are composed of a beta-hydroxy fatty acid linked to a peptide part comprising 10 amino acids, where 8 of them are organized in a cyclic structure. PPZPMs differ from members of the Viscosin and Amphisin group by the number of amino acids forming the cyclic structure. The two main components, PPZPM-1a and PPZPM-2a, were investigated additionally by means of NMR spectroscopy.
authors.forename: Hardy
authors.initials: H
authors.lastname: Weisshoff
authors.forename: Sarah
authors.initials: S
authors.lastname: Hentschel
authors.forename: Irmtraut
authors.initials: I
authors.lastname: Zaspel
authors.forename: René
authors.initials: R
authors.lastname: Jarling
authors.forename: Eberhard
authors.initials: E
authors.lastname: Krause
authors.forename: Thi Lam Huong
authors.initials: TL
authors.lastname: Pham
mesh_terms: D000595:Amino Acid Sequence; D047630:Depsipeptides / Q000737:chemistry* / Q000145:classification*; D015964:Gene Expression Regulation, Bacterial; D010456:Peptides, Cyclic / Q000737:chemistry*; D010838:Phytophthora / Q000382:microbiology* / Q000502:physiology; D011487:Protein Conformation; D011549:Pseudomonas / Q000502:physiology*
chemical_list: D047630:Depsipeptides; D010456:Peptides, Cyclic; C438267:amphisin; C062796:viscosin