title: Determination of the change of flavonoid components as the defence materials of Citrus unshiu Marc. fruit peel against Penicillium digitatum by liquid chromatography coupled with tandem mass spectrometry.
abstract: A healthy fruit peel of Citrus unshiu Marc. and one infected by Penicillium digitatum were analysed for flavonoids via high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) in the positive mode with selected ion monitoring (SIM). Among 16 flavonoid components characterised in C. unshiu Marc., four flavanones and nine flavones were identified for the first time. The identified compounds were quantified by HPLC-UV. To investigate the function of the flavonoids as defence materials, the flavonoid content change of the fruit peel inoculated with P. digitatum was monitored by HPLC. The flavonoid concentration in the infected fruit peel decreased initially after the infection and then gradually increased before finally progressively decreasing.
authors.affiliation: Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Hae Gyeong
authors.initials: HG
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: gonskim@gnu.ac.kr.
authors.forename: Gon-Sup
authors.initials: GS
authors.lastname: Kim
authors.affiliation: Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Jung Han
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Semin
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Won Young
authors.initials: WY
authors.lastname: Jeong
authors.affiliation: Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Yun-Hi
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Faculty of Biotechnology, Citrus Genetic Resources Bank, College of Applied Life Science, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: kimjh@cheju.ac.kr.
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Information and Statistics & Research Institute of Natural Science, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: kstioa@gnu.ac.kr.
authors.forename: Soo Taek
authors.initials: ST
authors.lastname: Kim
authors.affiliation: Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Young Ah
authors.initials: YA
authors.lastname: Cho
authors.affiliation: Department of Internal Medicine, Institute of Health Sciences, and Gyeongnam Regional Cancer Center, Gyeongsang National University, Jinju 660-702, Republic of Korea. Electronic address: Iwshmo@hanmail.net.
authors.forename: Won Sup
authors.initials: WS
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: bodry96@hanmail.net.
authors.forename: Soo Jung
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Korea Basic Science Institute Busan Center, Division of High Technology Materials Research, Gangseo-gu, Busan 618-230, Republic of Korea. Electronic address: jinjs55@naver.com.
authors.forename: Jong Sung
authors.initials: JS
authors.lastname: Jin
authors.affiliation: Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea. Electronic address: sshin@gnu.ac.kr.
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Shin
keywords: Citrus unshiu Marc.; Defence materials; Flavanones; Flavones; High-performance liquid chromatography; Tandem mass spectrometry