title: Bioinformatics in otolaryngology research. Part two: other high-throughput platforms in genomics and epigenetics.
abstract: OBJECTIVE
  This second segment of the two-part review summarises several modern high-throughput methods in genomics, epigenetics and molecular biology. Many principles from nucleotide sequencing and transcriptomics can be applied to other high-throughput molecular biology techniques. Specifically, this manuscript reviews: array comparative genome hybridisation; single nucleotide polymorphism arrays; microarray technology, used to study epigenetics; and methodology applied in proteomics. Finally, the review describes current methods for the integration of multiple molecular biology platforms.
  
  
  CONCLUSIONS
  Progress in treating human disease in general will require close collaboration with experts in bioinformatics. Improved understanding, by clinicians and physician-scientists in our field, of the concepts presented in both parts of this review will advance diagnosis and therapy for diseases of the head and neck.
authors.affiliation: Department of Otorhinolaryngology - Head and Neck Surgery,Montefiore Medical Center and Albert Einstein College of Medicine,Bronx,New York,USA.
authors.forename: T J
authors.initials: TJ
authors.lastname: Ow
authors.affiliation: Department of Pathology,Montefiore Medical Center and Albert Einstein College of Medicine,Bronx,New York,USA.
authors.forename: K
authors.initials: K
authors.lastname: Upadhyay
authors.affiliation: Department of Pathology,Montefiore Medical Center and Albert Einstein College of Medicine,Bronx,New York,USA.
authors.forename: T J
authors.initials: TJ
authors.lastname: Belbin
authors.affiliation: Department of Pathology,Montefiore Medical Center and Albert Einstein College of Medicine,Bronx,New York,USA.
authors.forename: M B
authors.initials: MB
authors.lastname: Prystowsky
authors.affiliation: Department of Pathology,Montefiore Medical Center and Albert Einstein College of Medicine,Bronx,New York,USA.
authors.forename: H
authors.initials: H
authors.lastname: Ostrer
authors.affiliation: Department of Otorhinolaryngology - Head and Neck Surgery,Montefiore Medical Center and Albert Einstein College of Medicine,Bronx,New York,USA.
authors.forename: R V
authors.initials: RV
authors.lastname: Smith
mesh_terms: D019295:Computational Biology / Q000379:methods*; D057890:Epigenomics / Q000379:methods*; D023281:Genomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010036:Otolaryngology / Q000379:methods*; D010038:Otorhinolaryngologic Diseases / Q000175:diagnosis / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D040901:Proteomics