title: Improvement of friable callus production of Boerhaavia paniculata Rich and the investigation of its lipid profile by GC/MS.
abstract: In this study, a protocol to induce high amount of friable callus of Boerhaavia paniculata RICH and a lipidomics technique were applied to investigate the profile of lipids to relate to those present in the roots of this plant that presented anti-inflammatory activity in the crude hexane extract. The callus culture was induced from seeds in solidified Murashige and Skoog medium containing different amounts of glucose and different concentrations of 2,4-Dichlorophenoxyacetic acid. The explants were kept in a germination chamber at 30±2°C with a photoperiod of 16 h under light intensity of 27 µmol m-2 s-1 for 4 weeks. The best results for friable callus formation and development of the biomass were obtained in the treatment containing 2.26 µM 2.4-D and glucose (1.5 %; w/v). Lipidomics techniques were applied in hexane fraction showing higher concentrations of the steroids β-sitosterol (3.53 mg/100 g dc-dry cells), and fatty acids, especially 2-hydroxy-tetracosanoic acid (0.34 mg/100 g dc), eicosanoic acid (86.25 mg/100 g dc), stearic acid (420.83 mg/100 g dc), tetradecanoic acid (10.74 mg/100 g dc) and linoleic acid (100.61 mg/100 g dc). The lipid profile of callus versus that found in the roots of wild plant is described in this work.
authors.affiliation: Laboratório de Investigação Sistemática em Biotecnologia e Biodiversidade Molecular/LabISisBio, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: Joanne M M
authors.initials: JM
authors.lastname: Souza
authors.affiliation: AgroBioInstitute, Sofia, Bulgaria.
authors.forename: Strahill
authors.initials: S
authors.lastname: Berkov
authors.affiliation: Laboratório de Investigação Sistemática em Biotecnologia e Biodiversidade Molecular/LabISisBio, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: Alberdan S
authors.initials: AS
authors.lastname: Santos
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry; D018525:Germination; D008055:Lipids / Q000032:analysis*; D039281:Nyctaginaceae / Q000737:chemistry* / Q000145:classification / Q000254:growth & development; D018517:Plant Roots / Q000737:chemistry*
chemical_list: D008055:Lipids