title: Fast simultaneous determination of prominent polyphenols in vegetables and fruits by reversed phase liquid chromatography using a fused-core column.
abstract: A reversed-phase high-performance liquid chromatography method with photodiode array detection has been developed enabling the joint determination of 17 prominent flavonoids and phenolic acids in vegetables and fruits. A multi-segmented gradient program using a fused-core column for the separation of several phenolic classes (phenolic acids and flavonoids) has been optimised. The influence of extraction conditions (sample freeze-drying, ultrasound extraction, solvent composition and extraction time) has been also optimised using response surface methodology with tomato samples as a model. Complete recoveries (76-108%) were obtained for the phenolic compounds present in tomato. The developed method provided satisfactory repeatability in terms of peak area (RSD<2.9%) and retention time (RSD<0.2%) both for standards and real samples. Detection limits ranged between 3 and 44μgkg(-1) for the detected polyphenols. This method is recommended for routine analysis of large number of samples typical of production quality systems or plant breeding programs.
authors.affiliation: Department de Ciències Agràries i del Medi Natural, Universitat Jaume I, Avda. Sos Baynat s/n, 12071 Castellón, Spain.
authors.forename: Raúl
authors.initials: R
authors.lastname: Martí
authors.affiliation: Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana (COMAV), Universitat Politècnica de València, Cno. de Vera s/n, 46022 València, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Valcárcel
authors.affiliation: Departamento de Química Analítica, Universitat de València, Dr. Moliner, 50, 46100 Burjassot, Spain.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Herrero-Martínez
authors.affiliation: Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana (COMAV), Universitat Politècnica de València, Cno. de Vera s/n, 46022 València, Spain.
authors.forename: Jaime
authors.initials: J
authors.lastname: Cebolla-Cornejo
authors.affiliation: Department de Ciències Agràries i del Medi Natural, Universitat Jaume I, Avda. Sos Baynat s/n, 12071 Castellón, Spain. Electronic address: rosello@uji.es.
authors.forename: Salvador
authors.initials: S
authors.lastname: Roselló
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D005419:Flavonoids / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D057230:Limit of Detection; D010636:Phenols / Q000032:analysis; D059808:Polyphenols / Q000032:analysis*; D014675:Vegetables / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols
keywords: Food; Functional quality; HPLC-DAD; Response surface methodology; Ultrasound-assisted extraction