title: Cytotoxic granules in distinct subsets of cutaneous lupus erythematosus.
abstract: In cutaneous lupus erythematosus (CLE), the pathogenetic role of cytotoxic granules has been demonstrated in the subacute and discoid subtypes, which show interface dermatitis, but little is known about tumid (T)CLE, which does not show this interface dermatitis, and evolves with minimal epidermal changes. We studied cytotoxic T lymphocytes and cytotoxic granules in discoid (n = 21), subacute (n = 17), and tumid (n = 21) CLE samples. Skin sections were immunohistochemically stained for CD8, CD56, perforin, granzyme A, granzyme B, and granulysin. Inflammatory cells containing the four subtypes of cytotoxic granules were found in all the three CLE forms; however, only the TCLE group showed a positive correlation between the density of CD8+ cells and each subtype of cytotoxic granule-positive cells. In addition, only the TCLE group showed synergy between the densities of cells containing cytotoxic granule subtypes. Cytotoxic granules are important in the pathomechanism of TCLE. They may perform functions other than apoptosis, including maintenance of inflammation and dermal mucinous deposits in TCLE.
authors.affiliation: Department of Pathology, Faculty of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: L
authors.initials: L
authors.lastname: Fogagnolo
authors.forename: T C B
authors.initials: TC
authors.lastname: Soares
authors.forename: C G
authors.initials: CG
authors.lastname: Senna
authors.forename: E M
authors.initials: EM
authors.lastname: Souza
authors.forename: M H S L
authors.initials: MH
authors.lastname: Blotta
authors.forename: M L
authors.initials: ML
authors.lastname: Cintra
mesh_terms: D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D019002:CD56 Antigen / Q000032:analysis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002452:Cell Count; D053804:Granzymes / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008178:Lupus Erythematosus, Cutaneous / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D054353:Perforin / Q000378:metabolism; D012189:Retrospective Studies; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D019002:CD56 Antigen; C075005:GNLY protein, human; D054353:Perforin; D053804:Granzymes