title: Multi-colored immunochromatography using nanobeads for rapid and sensitive typing of seasonal influenza viruses.
abstract: Immunochromatography (IC) is an antigen-detection assay that plays an important role in the rapid diagnosis of influenza viruses because of its rapid turnaround and ease of use. Despite the usefulness of IC, the limit of detection of common IC kits is as high as 10(3)-10(4) plaque forming units (pfu) per reaction, resulting in their limited sensitivities. Early diagnosis within 24h would provide more appropriate timing of treatment. In this study, a multi-colored NanoAct™ bead IC was established to detect seasonal influenza viruses. This method has approximately 10-fold higher sensitivity than that of colloidal gold or colored latex bead IC assays, and does not require specific instruments. More notably, NanoAct™ bead IC can distinguish influenza A and B viruses from clinical samples with a straightforward readout composed of colored lines. Our results will provide new strategies for the diagnosis, treatment, and a chance to survey of influenza viruses in developing countries and in the field research.
authors.affiliation: Department of Molecular Medical Research, Tokyo Metropolitan Institute of Medical Science, 2-1-6, Kamikitazawa, Setagaya-ku, Tokyo 156-8506, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Sakurai
authors.affiliation: ADTEC Co., Ltd., 1693-6 Yokkaichi, Usa-City, Oita 879-0471, Japan.
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Takayama
authors.affiliation: Department of Molecular Medical Research, Tokyo Metropolitan Institute of Medical Science, 2-1-6, Kamikitazawa, Setagaya-ku, Tokyo 156-8506, Japan.
authors.forename: Namiko
authors.initials: N
authors.lastname: Nomura
authors.affiliation: Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, N18 W9, Kita-ku, Sapporo 060-0818, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.affiliation: Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, N18 W9, Kita-ku, Sapporo 060-0818, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sakoda
authors.affiliation: ADTEC Co., Ltd., 1693-6 Yokkaichi, Usa-City, Oita 879-0471, Japan.
authors.forename: Yukuharu
authors.initials: Y
authors.lastname: Kobayashi
authors.affiliation: Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, N18 W9, Kita-ku, Sapporo 060-0818, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kida
authors.affiliation: Department of Molecular Medical Research, Tokyo Metropolitan Institute of Medical Science, 2-1-6, Kamikitazawa, Setagaya-ku, Tokyo 156-8506, Japan. Electronic address: shibasaki-ft@igakuken.or.jp.
authors.forename: Futoshi
authors.initials: F
authors.lastname: Shibasaki
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003116:Color; D006801:Humans; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology*; D053758:Nanoparticles; D009975:Orthomyxoviridae / Q000145:classification* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013997:Time Factors
keywords: Diagnosis; Immunochromatography; Influenza viruses; Nanobead