title: Electrical immunosensor based on dielectrophoretically-deposited carbon nanotubes for detection of influenza virus H1N1.
abstract: The influenza virus has received extensive attention due to the recent H1N1 pandemics originating from swine. This study reports a label-free, highly sensitive, and selective electrical immunosensor for the detection of influenza virus H1N1 based on dielectrophoretically deposited single-walled carbon nanotubes (SWCNTs). COOH-functionalized SWCNTs were deposited on a self-assembled monolayer of polyelectrolyte polydiallyldimethyl-ammonium chloride (PDDA) between two gold electrodes by dielectrophoretic and electrostatic forces, which resulted in reproducible, uniform, aligned, and aggregation-free SWCNT channels (2-10 μm in length). Avidin was immobilized onto the PDDA-SWCNT channels, and viral antibodies were immobilized using biotin-avidin coupling. The resistance of the channels increased with the binding of the influenza viruses to the antibodies. These immunosensors showed linear behavior as the virus concentration was varied from 1 to 10(4) PFU ml(-1) along with a detection time of 30 min. The immunosensors with a 2 μm channel length detected 1 PFU ml(-1) of the influenza virus accurately (R(2) = 0.99) and selectively from MS2 bacteriophages. These immunosensors have the potential to become an important component of a point-of-care test kit that will enable a rapid clinical diagnosis.
authors.affiliation: School of Mechanical and Nuclear Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 689-798, Republic of Korea. jjang@unist.ac.kr.
authors.forename: Renu
authors.initials: R
authors.lastname: Singh
authors.forename: Abhinav
authors.initials: A
authors.lastname: Sharma
authors.forename: Seongkyeol
authors.initials: S
authors.lastname: Hong
authors.forename: Jaesung
authors.initials: J
authors.lastname: Jang
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis*; D015374:Biosensing Techniques; D004586:Electrophoresis / Q000379:methods*; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology; D008561:Membrane Fusion; D008839:Microelectrodes; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D037742:Nanotubes, Carbon; D055672:Static Electricity
chemical_list: D000914:Antibodies, Viral; D037742:Nanotubes, Carbon