title: Oxidative stress mediated cytotoxicity of biologically synthesized silver nanoparticles in human lung epithelial adenocarcinoma cell line.
abstract: The goal of the present study was to investigate the toxicity of biologically prepared small size of silver nanoparticles in human lung epithelial adenocarcinoma cells A549. Herein, we describe a facile method for the synthesis of silver nanoparticles by treating the supernatant from a culture of Escherichia coli with silver nitrate. The formation of silver nanoparticles was characterized using various analytical techniques. The results from UV-visible (UV-vis) spectroscopy and X-ray diffraction analysis show a characteristic strong resonance centered at 420 nm and a single crystalline nature, respectively. Fourier transform infrared spectroscopy confirmed the possible bio-molecules responsible for the reduction of silver from silver nitrate into nanoparticles. The particle size analyzer and transmission electron microscopy results suggest that silver nanoparticles are spherical in shape with an average diameter of 15 nm. The results derived from in vitro studies showed a concentration-dependent decrease in cell viability when A549 cells were exposed to silver nanoparticles. This decrease in cell viability corresponded to increased leakage of lactate dehydrogenase (LDH), increased intracellular reactive oxygen species generation (ROS), and decreased mitochondrial transmembrane potential (MTP). Furthermore, uptake and intracellular localization of silver nanoparticles were observed and were accompanied by accumulation of autophagosomes and autolysosomes in A549 cells. The results indicate that silver nanoparticles play a significant role in apoptosis. Interestingly, biologically synthesized silver nanoparticles showed more potent cytotoxicity at the concentrations tested compared to that shown by chemically synthesized silver nanoparticles. Therefore, our results demonstrated that human lung epithelial A549 cells could provide a valuable model to assess the cytotoxicity of silver nanoparticles.
authors.affiliation: Department of Animal Biotechnology, Konkuk University, 1 Hwayang-Dong, Gwangin-gu Seoul 143-701, Korea.
authors.forename: Jae Woong
authors.initials: JW
authors.lastname: Han
authors.affiliation: Department of Animal Biotechnology, Konkuk University, 1 Hwayang-Dong, Gwangin-gu Seoul 143-701, Korea ; GS Institute of Bio and Nanotechnology, Coimbatore, Tamilnadu 641024, India.
authors.forename: Sangiliyandi
authors.initials: S
authors.lastname: Gurunathan
authors.affiliation: Department of Animal Biotechnology, Konkuk University, 1 Hwayang-Dong, Gwangin-gu Seoul 143-701, Korea.
authors.forename: Jae-Kyo
authors.initials: JK
authors.lastname: Jeong
authors.affiliation: Department of Animal Biotechnology, Konkuk University, 1 Hwayang-Dong, Gwangin-gu Seoul 143-701, Korea.
authors.forename: Yun-Jung
authors.initials: YJ
authors.lastname: Choi
authors.affiliation: Department of Animal Biotechnology, Konkuk University, 1 Hwayang-Dong, Gwangin-gu Seoul 143-701, Korea.
authors.forename: Deug-Nam
authors.initials: DN
authors.lastname: Kwon
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Suwon 441-350, Korea.
authors.forename: Jin-Ki
authors.initials: JK
authors.lastname: Park
authors.affiliation: Department of Animal Biotechnology, Konkuk University, 1 Hwayang-Dong, Gwangin-gu Seoul 143-701, Korea.
authors.forename: Jin-Hoi
authors.initials: JH
authors.lastname: Kim
keywords: Adenocarcinoma cells A549; Lactate dehydrogenase (LDH); Mitochondrial transmembrane potential (MTP); Reactive oxygen species generation (ROS); Silver nanoparticles (AgNP)