title: An eight-parent multiparent advanced generation inter-cross population for winter-sown wheat: creation, properties, and validation.
abstract: MAGIC populations represent one of a new generation of crop genetic mapping resources combining high genetic recombination and diversity. We describe the creation and validation of an eight-parent MAGIC population consisting of 1091 F7 lines of winter-sown wheat (Triticum aestivum L.). Analyses based on genotypes from a 90,000-single nucleotide polymorphism (SNP) array find the population to be well-suited as a platform for fine-mapping quantitative trait loci (QTL) and gene isolation. Patterns of linkage disequilibrium (LD) show the population to be highly recombined; genetic marker diversity among the founders was 74% of that captured in a larger set of 64 wheat varieties, and 54% of SNPs segregating among the 64 lines also segregated among the eight founder lines. In contrast, a commonly used reference bi-parental population had only 54% of the diversity of the 64 varieties with 27% of SNPs segregating. We demonstrate the potential of this MAGIC resource by identifying a highly diagnostic marker for the morphological character "awn presence/absence" and independently validate it in an association-mapping panel. These analyses show this large, diverse, and highly recombined MAGIC population to be a powerful resource for the genetic dissection of target traits in wheat, and it is well-placed to efficiently exploit ongoing advances in phenomics and genomics. Genetic marker and trait data, together with instructions for access to seed, are available at http://www.niab.com/MAGIC/.
authors.affiliation: The John Bingham Laboratory, National Institute of Agricultural Botany (NIAB), Cambridge, CB3 0LE, United Kingdom ian.mackay@niab.com.
authors.forename: Ian J
authors.identifier: http://orcid.org/0000-0002-2605-2314
authors.initials: IJ
authors.lastname: Mackay
authors.affiliation: The John Bingham Laboratory, National Institute of Agricultural Botany (NIAB), Cambridge, CB3 0LE, United Kingdom.
authors.forename: Pauline
authors.initials: P
authors.lastname: Bansept-Basler
authors.affiliation: The John Bingham Laboratory, National Institute of Agricultural Botany (NIAB), Cambridge, CB3 0LE, United Kingdom.
authors.forename: Toby
authors.initials: T
authors.lastname: Barber
authors.affiliation: The John Bingham Laboratory, National Institute of Agricultural Botany (NIAB), Cambridge, CB3 0LE, United Kingdom.
authors.forename: Alison R
authors.initials: AR
authors.lastname: Bentley
authors.affiliation: The John Bingham Laboratory, National Institute of Agricultural Botany (NIAB), Cambridge, CB3 0LE, United Kingdom.
authors.forename: James
authors.initials: J
authors.lastname: Cockram
authors.affiliation: The John Bingham Laboratory, National Institute of Agricultural Botany (NIAB), Cambridge, CB3 0LE, United Kingdom.
authors.forename: Nick
authors.initials: N
authors.lastname: Gosman
authors.affiliation: The John Bingham Laboratory, National Institute of Agricultural Botany (NIAB), Cambridge, CB3 0LE, United Kingdom.
authors.forename: Andy J
authors.initials: AJ
authors.lastname: Greenland
authors.affiliation: The John Bingham Laboratory, National Institute of Agricultural Botany (NIAB), Cambridge, CB3 0LE, United Kingdom.
authors.forename: Richard
authors.initials: R
authors.lastname: Horsnell
authors.affiliation: The John Bingham Laboratory, National Institute of Agricultural Botany (NIAB), Cambridge, CB3 0LE, United Kingdom.
authors.forename: Rhian
authors.initials: R
authors.lastname: Howells
authors.affiliation: The John Bingham Laboratory, National Institute of Agricultural Botany (NIAB), Cambridge, CB3 0LE, United Kingdom.
authors.forename: Donal M
authors.initials: DM
authors.lastname: O'Sullivan
authors.affiliation: The John Bingham Laboratory, National Institute of Agricultural Botany (NIAB), Cambridge, CB3 0LE, United Kingdom.
authors.forename: Gemma A
authors.initials: GA
authors.lastname: Rose
authors.affiliation: The John Bingham Laboratory, National Institute of Agricultural Botany (NIAB), Cambridge, CB3 0LE, United Kingdom.
authors.forename: Phil J
authors.initials: PJ
authors.lastname: Howell
mesh_terms: D002874:Chromosome Mapping; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance; D035264:Flowers / Q000502:physiology; D005787:Gene Frequency; D018745:Genome, Plant; D005838:Genotype; D015810:Linkage Disequilibrium; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D015203:Reproducibility of Results; D036226:Seedlings / Q000235:genetics; D014908:Triticum / Q000235:genetics* / Q000502:physiology
chemical_list: D018744:DNA, Plant
keywords: MPP; Multiparent Advanced Generation Inter-Cross (MAGIC); Multiparental populations; QTL mapping; genome-wide association scan; high-density genotyping; quantitative genetics; recombinant inbred line (RIL); single nucleotide polymorphism