title: Unique antibody responses to malondialdehyde-acetaldehyde (MAA)-protein adducts predict coronary artery disease.
abstract: Malondialdehyde-acetaldehyde adducts (MAA) have been implicated in atherosclerosis. The purpose of this study was to investigate the role of MAA in atherosclerotic disease. Serum samples from controls (n = 82) and patients with; non-obstructive coronary artery disease (CAD), (n = 40), acute myocardial infarction (AMI) (n = 42), or coronary artery bypass graft (CABG) surgery due to obstructive multi-vessel CAD (n = 72), were collected and tested for antibody isotypes to MAA-modifed human serum albumin (MAA-HSA). CAD patients had elevated relative levels of IgG and IgA anti-MAA, compared to control patients (p<0.001). AMI patients had a significantly increased relative levels of circulating IgG anti-MAA-HSA antibodies as compared to stable angina (p<0.03) or CABG patients (p<0.003). CABG patients had significantly increased relative levels of circulating IgA anti-MAA-HSA antibodies as compared to non-obstructive CAD (p<0.001) and AMI patients (p<0.001). Additionally, MAA-modified proteins were detected in the tissue of human AMI lesions. In conclusion, the IgM, IgG and IgA anti-MAA-HSA antibody isotypes are differentially and significantly associated with non-obstructive CAD, AMI, or obstructive multi-vessel CAD and may serve as biomarkers of atherosclerotic disease.
authors.affiliation: Department of Internal Medicine, Division of Cardiology, University of Nebraska Medical Center, Omaha, NE, United States of America.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Anderson
authors.affiliation: Veterans Affairs Nebraska-Western Iowa Health Care System, Research Service, Omaha, NE, United States of America; Department of Internal Medicine, Division of Rheumatology, University of Nebraska Medical Center, Omaha, NE, United States of America.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Duryee
authors.affiliation: Department of Internal Medicine, Division of Cardiology, University of Nebraska Medical Center, Omaha, NE, United States of America.
authors.forename: Scott W
authors.initials: SW
authors.lastname: Shurmur
authors.affiliation: Department of Surgery, Division of Cardiothoracic Surgery, University of Nebraska Medical Center, Omaha, NE, United States of America.
authors.forename: John Y
authors.initials: JY
authors.lastname: Um
authors.affiliation: Department of Surgery, Division of Cardiothoracic Surgery, University of Nebraska Medical Center, Omaha, NE, United States of America.
authors.forename: Walter D
authors.initials: WD
authors.lastname: Bussey
authors.affiliation: Veterans Affairs Nebraska-Western Iowa Health Care System, Research Service, Omaha, NE, United States of America; Department of Internal Medicine, Division of Rheumatology, University of Nebraska Medical Center, Omaha, NE, United States of America.
authors.forename: Carlos D
authors.initials: CD
authors.lastname: Hunter
authors.affiliation: Department of Internal Medicine, Division of Cardiology, University of Nebraska Medical Center, Omaha, NE, United States of America.
authors.forename: Robert P
authors.initials: RP
authors.lastname: Garvin
authors.affiliation: Department of Internal Medicine, Division of Rheumatology, University of Nebraska Medical Center, Omaha, NE, United States of America.
authors.forename: Harlan R
authors.initials: HR
authors.lastname: Sayles
authors.affiliation: Veterans Affairs Nebraska-Western Iowa Health Care System, Research Service, Omaha, NE, United States of America; Department of Internal Medicine, Division of Rheumatology, University of Nebraska Medical Center, Omaha, NE, United States of America.
authors.forename: Ted R
authors.initials: TR
authors.lastname: Mikuls
authors.affiliation: Veterans Affairs Nebraska-Western Iowa Health Care System, Research Service, Omaha, NE, United States of America; Department of Internal Medicine, Division of Rheumatology, University of Nebraska Medical Center, Omaha, NE, United States of America.
authors.forename: Lynell W
authors.initials: LW
authors.lastname: Klassen
authors.affiliation: Veterans Affairs Nebraska-Western Iowa Health Care System, Research Service, Omaha, NE, United States of America; Department of Internal Medicine, Division of Rheumatology, University of Nebraska Medical Center, Omaha, NE, United States of America; Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, United States of America.
authors.forename: Geoffrey M
authors.initials: GM
authors.lastname: Thiele
mesh_terms: D000079:Acetaldehyde / Q000276:immunology*; D000368:Aged; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D005260:Female; D006801:Humans; D008077:Lipoproteins, LDL / Q000276:immunology*; D008297:Male; D008315:Malondialdehyde / Q000276:immunology*; D008875:Middle Aged; D058226:Plaque, Atherosclerotic / Q000097:blood / Q000276:immunology
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D008077:Lipoproteins, LDL; D008315:Malondialdehyde; D000079:Acetaldehyde