title: The loss of CD34+ cells in peripheral hematopoietic stem cell products cryopreserved by non-controlled rate freezing and stored at -80 °C after overnight storage.
abstract: Although peripheral blood stem cell (PBSC) products cryopreserved by non-controlled rate freezing and stored at -80 °C after overnight storage are used frequently, data regarding the rate of loss of CD34+ cells in these products are limited. In this prospective study, CD34+ cells were counted at three (fresh, post-overnight and post-thaw) points in 83 PBSC products from 41 patients by flow cytometry. Compared to fresh products, the mean losses of post-overnight and post-thaw total CD34+ cells are 16.3% and 38.4% (p = 0.02), and the mean losses of post-overnight and post-thaw viable CD34+ cells are 16.5% and 48.5%, respectively (p < 0.001). The numbers of fresh viable, post-thaw total and post-thaw viable CD34+ cells were inversely correlated with the durations of neutrophil and platelet engraftment. Our results indicate that the mean loss of post-thaw total and viable CD34+ cells is approximately 20% higher than that observed in standard cryopreservation methods. In addition, fresh viable, post-thaw total and especially post-thaw viable CD34+ cell levels are valuable predictors of both neutrophil and platelet engraftments.
authors.affiliation: Department of Hematology, Ege University Medical School, Izmir, Turkey. Electronic address: ayhan.donmez@ege.edu.tr.
authors.forename: Ayhan
authors.initials: A
authors.lastname: Donmez
authors.affiliation: Department of Hematology, Ege University Medical School, Izmir, Turkey.
authors.forename: Fergun
authors.initials: F
authors.lastname: Yilmaz
authors.affiliation: Department of Hematology, Ege University Medical School, Izmir, Turkey.
authors.forename: Nur
authors.initials: N
authors.lastname: Soyer
authors.affiliation: Department of Hematology, Ege University Medical School, Izmir, Turkey.
authors.forename: Seckin
authors.initials: S
authors.lastname: Cagirgan
authors.affiliation: Department of Hematology, Ege University Medical School, Izmir, Turkey.
authors.forename: Bahar
authors.initials: B
authors.lastname: Arik
authors.affiliation: Department of Hematology, Ege University Medical School, Izmir, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Tombuloglu
mesh_terms: D000328:Adult; D000686:Amyloidosis / Q000097:blood / Q000628:therapy*; D018952:Antigens, CD34 / Q000378:metabolism*; D064592:Autografts; D015925:Cryopreservation; D005260:Female; D006085:Graft Survival; D019337:Hematologic Neoplasms / Q000097:blood / Q000628:therapy*; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation; D011446:Prospective Studies; D013997:Time Factors
chemical_list: D018952:Antigens, CD34
keywords: CD34+ cells; Cryopreservation; Overnight storage; Peripheral blood stem cell