title: Fufang Kushen injection inhibits sarcoma growth and tumor-induced hyperalgesia via TRPV1 signaling pathways.
abstract: Cancer pain is a deleterious consequence of tumor growth and related inflammation. Opioids and anti-inflammatory drugs provide first line treatment for cancer pain, but both are limited by side effects. Fufang Kushen injection (FKI) is GMP produced, traditional Chinese medicine used alone or with chemotherapy to reduce cancer-associated pain. FKI limited mouse sarcoma growth both in vivo and in vitro, in part, by reducing the phosphorylation of ERK and AKT kinases and BAD. FKI inhibited TRPV1 mediated capsaicin-induced ERK phosphorylation and reduced tumor-induced proinflammatory cytokine production. Thus, FKI limited cancer pain both directly by blocking TRPV1 signaling and indirectly by reducing tumor growth.
authors.affiliation: Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute-Frederick, Frederick, MD 21702, USA; Guang An Men Hospital of China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Zhizheng
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute-Frederick, Frederick, MD 21702, USA; Guang An Men Hospital of China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Huiting
authors.initials: H
authors.lastname: Fan
authors.affiliation: Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute-Frederick, Frederick, MD 21702, USA.
authors.forename: Tim
authors.initials: T
authors.lastname: Higgins
authors.affiliation: Neuronal Networks Section, Intramural Research Program, National Institute on Drug Abuse, NIH, 251 Bayview Blvd, Baltimore, MD 21224, USA.
authors.forename: Jia
authors.initials: J
authors.lastname: Qi
authors.affiliation: Leidos Biomedical, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA.
authors.forename: Diana
authors.initials: D
authors.lastname: Haines
authors.affiliation: Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute-Frederick, Frederick, MD 21702, USA.
authors.forename: Anna
authors.initials: A
authors.lastname: Trivett
authors.affiliation: Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute-Frederick, Frederick, MD 21702, USA.
authors.forename: Joost J
authors.initials: JJ
authors.lastname: Oppenheim
authors.affiliation: Guang An Men Hospital of China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Hou
authors.initials: H
authors.lastname: Wei
authors.affiliation: Guang An Men Hospital of China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Guang An Men Hospital of China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Lin
authors.affiliation: Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute-Frederick, Frederick, MD 21702, USA. Electronic address: howardz@mail.nih.gov.
authors.forename: O M Zack
authors.initials: OM
authors.lastname: Howard
mesh_terms: D000818:Animals; D002211:Capsaicin / Q000378:metabolism; D045744:Cell Line, Tumor; D016207:Cytokines / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006930:Hyperalgesia / Q000188:drug therapy* / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D012509:Sarcoma / Q000150:complications* / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism; D050916:TRPV Cation Channels / Q000378:metabolism*
chemical_list: D016207:Cytokines; D004365:Drugs, Chinese Herbal; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; C574916:fufangkushen; D051057:Proto-Oncogene Proteins c-akt; D002211:Capsaicin
keywords: ERK; Fufang Kushen injection; Hyperalgesia; Sarcoma; TRPV1