title: Planifilum caeni sp. nov., a novel member of thermoactinomycete isolated from sludge compost.
abstract: A Gram positive, thermophilic, and filamentous bacterium, designated strain GIESS004(T), was isolated from a sludge compost from Guangzhou, China. Growth occurred at 45-55 °C (optimum 50-53 °C) and pH 6.0-10.0 (optimum pH 7.0-7.5). The organism formed cream-yellow colonies with radial wrinkles. Aerial mycelium was not produced in any of the growth media tested. Single spores were produced along the substrate hyphae. Strain GIESS004(T) contained MK-7 as the predominant menaquinone and iso-C(16:0), iso-C(15:0) and iso-C(17:0) as the major cellular fatty acids. The cell wall contained meso-diaminopimelic acid. The DNA G+C content was 60.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GIESS004(T) was associated with the genus Planifilum and formed a cluster with its closest relative of Planifilum composti P8(T) (99.5 % similarity). However, the DNA-DNA pairing study showed that the isolate displayed reassociation value of only 57.6 ± 2.3 % with P. composti P8(T). On the basis of the polyphasic taxonomic results, strain GIESS004(T) (= CCTCC AB2013107(T) = MCCC 1K00414(T)) is designated as the type strain of a novel species of the genus Planifilum, for which the name Planifilum caeni sp. nov. is proposed.
authors.affiliation: Guangdong Institute of Eco-environmental and Soil Sciences, Guangzhou, 510650, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yu
authors.forename: Chu
authors.initials: C
authors.lastname: Wu
authors.forename: Gui-Qin
authors.initials: GQ
authors.lastname: Yang
authors.forename: Shun-Gui
authors.initials: SG
authors.lastname: Zhou
mesh_terms: D056449:Bacillales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D012722:Sewage / Q000382:microbiology*; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012722:Sewage