title: Forward Vaccinology: CTL Targeting Based upon Physical Detection of HLA-Bound Peptides.
abstract: Vaccine-elicited cytotoxic T lymphocytes (CTL) recognizing conserved fragments of a pathogen's proteome could greatly impact infectious diseases and cancers. Enabling this potential are recent advances in mass spectrometry that identify specific target peptides among the myriad HLA-bound peptides on altered cells. Ultrasensitivity of these physical detection methods allows for the direct assessment of peptide presentation on small numbers of tissue-derived cells. In addition, concurrent advances in immunobiology suggest ways to induce CTLs with requisite functional avidity and tissue deployment. Elicitation of high-avidity resident-memory T cells through vaccination may shift the vaccinology paradigm both for preventive and therapeutic approaches to human disease control.
authors.affiliation: Laboratory of Immunobiology, Department of Medical Oncology, Dana-Farber Cancer Institute , Boston, MA , USA ; Department of Medicine, Harvard Medical School , Boston, MA , USA.
authors.forename: Ellis L
authors.initials: EL
authors.lastname: Reinherz
authors.affiliation: Laboratory of Immunobiology, Department of Medical Oncology, Dana-Farber Cancer Institute , Boston, MA , USA ; Department of Medicine, Harvard Medical School , Boston, MA , USA.
authors.forename: Derin B
authors.initials: DB
authors.lastname: Keskin
authors.affiliation: Laboratory of Immunobiology, Department of Medical Oncology, Dana-Farber Cancer Institute , Boston, MA , USA ; Department of Medicine, Harvard Medical School , Boston, MA , USA.
authors.forename: Bruce
authors.initials: B
authors.lastname: Reinhold
keywords: CTL; T cell epitopes; TCR; mass spectrometry; proteomics; resident-memory T cells; vaccines