title: Major histocompatibility complex class I and class II alleles may confer susceptibility to or protection against morphea: findings from the Morphea in Adults and Children cohort.
abstract: OBJECTIVE
  To determine the HLA class I and class II alleles of the human major histocompatibility complex showing an association with morphea (localized scleroderma) in the Morphea in Adults and Children (MAC) cohort, using a nested case-control association study.
  
  
  METHODS
  Patients with morphea were identified from the MAC cohort, and matched controls were obtained from the National Institutes of Health/National Institute of Arthritis and Musculoskeletal and Skin Diseases Scleroderma Family Registry and DNA Repository and from the Division of Rheumatology at the University of Texas Health Science Center at Houston. HLA class II genotyping and single-strand conformational polymorphism typing were performed to identify HLA-A, B, and C alleles. Associations between HLA class I and class II alleles and morphea, as well as its subphenotypes, were determined.
  
  
  RESULTS
  Two hundred eleven patients with morphea and 726 matched controls were available for HLA class I typing, and 158 patients with morphea and 1,008 matched controls were available for HLA class II typing. The strongest associations were found with DRB1*04:04 (odds ratio [OR] 2.3, 95% confidence interval [95% CI] 1.4-4.0, P = 0.002), and HLA-B*37 conferred the highest OR among the class I alleles (OR 3.2, 95% CI 1.5-6.5, P = 0.001). Comparison of the risk allele profile in this cohort with the risk alleles previously identified in patients with systemic sclerosis, determined using the same methods and same control population, revealed one allele in common, DRB*04:04.
  
  
  CONCLUSIONS
  These results demonstrate that specific HLA class I and class II alleles are associated with morphea and are also likely to be associated with generalized and linear subtypes of morphea. The morphea-associated alleles are different from those found in scleroderma, suggesting that morphea is immunogenetically distinct. Risk alleles in morphea are also associated with conditions such as rheumatoid arthritis (RA) and other autoimmune conditions. Population-based studies have indicated that patients with RA have an increased risk of morphea, and therefore a common susceptibility allele may be implicated.
authors.affiliation: University of Texas Southwestern Medical Center, Dallas.
authors.forename: Heidi
authors.initials: H
authors.lastname: Jacobe
authors.forename: Chul
authors.initials: C
authors.lastname: Ahn
authors.forename: Frank C
authors.initials: FC
authors.lastname: Arnett
authors.forename: John D
authors.initials: JD
authors.lastname: Reveille
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D000974:Antibodies, Antinuclear / Q000097:blood; D016022:Case-Control Studies; D002648:Child; D015331:Cohort Studies; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005805:Genes, MHC Class I / Q000235:genetics*; D005802:Genes, MHC Class II / Q000235:genetics*; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D059811:HLA-DRB1 Chains / Q000235:genetics; D006801:Humans; D008297:Male; D012042:Registries; D012307:Risk Factors; D012594:Scleroderma, Localized / Q000097:blood / Q000235:genetics*; D055815:Young Adult
chemical_list: D000974:Antibodies, Antinuclear; C488436:HLA DRB1*04:04 antigen; D059811:HLA-DRB1 Chains