title: β-1,4-galactosyltransferase-I activates proliferation and participates in intercellular contacts of lymphocytes.
abstract: Beta-1,4-galactosyltransferase I (β-1,4-GalT-I) mediates several biological mechanisms as a key enzyme in glycobiology, but the role of β-1,4-GalT-I in immune system remains unclear. We conducted experiments to investigate whether β-1,4-GalT-I is participating in the activation of Jurkat T cell and intercellular contacts formed by co-incubation of Jurkat and Raji cells in the presence of the superantigen staphylococcal enterotoxin B (SEB). Jurkat cells were also activated by CD3/CD28 co-stimulation in order to examine the expression pattern and cellular distribution of β-1,4-GalT-I upon stimulation. Here we found that β-1,4-GalT-I expression was enhanced and rearranged on the cell surface of activated Jurkat cells. Further, immunofluorescence staining assays revealed that β-1,4-GalT-I co-aggregated with CD3 and CD28 molecules in the junction sides of Jurkat and SEB pulsed Raji cells. Our study also showed that silencing of β-1,4-GalT-I inhibited Jurkat activated Raji cell conjugations and reduced the proliferation rate of activated Jurkat cells. Our data provide evidence that β-1,4-GalT-I plays a role in activation of Jurkat T lymphocytes and participates in intercellular contact formation.
authors.affiliation: Department of Immunology, Nantong University, Nantong, Jiangsu, PR China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Immunology, Nantong University, Nantong, Jiangsu, PR China; Department of Basic Medical Research Centre, Nantong University, Nantong, Jiangsu, PR China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Immunology, Nantong University, Nantong, Jiangsu, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Immunology, Nantong University, Nantong, Jiangsu, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Xue
authors.affiliation: Department of Human Anatomy, Nantong University, Nantong, Jiangsu, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Department of Immunology, Nantong University, Nantong, Jiangsu, PR China.
authors.forename: Gie
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Immunology, Nantong University, Nantong, Jiangsu, PR China.
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Immunology, Nantong University, Nantong, Jiangsu, PR China. Electronic address: wangxiaoying_nt@163.com.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wang
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology*; D018106:CD28 Antigens / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D002450:Cell Communication / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D004768:Enterotoxins / Q000276:immunology; D005700:Galactosyltransferases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D008213:Lymphocyte Activation / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D018106:CD28 Antigens; D017252:CD3 Complex; D004768:Enterotoxins; D034741:RNA, Small Interfering; C031818:enterotoxin B, staphylococcal; D005700:Galactosyltransferases; C119701:beta-1,4-galactosyltransferase I
keywords: Immunological synapse; Intercellular contact; Jurkat cell; β-1,4-GalT-I