title: Combining chemical sequential extractions with 3D fluorescence spectroscopy to characterize sludge organic matter.
abstract: The design and management of anaerobic digestion of sewage sludge (SS) require a relevant characterisation of the sludge organic matter (OM). Methods currently used are time-consuming and often insufficiently informative. A new method combining chemical sequential extractions (CSE) with 3D fluorescence spectroscopy was developed to provide a relevant SS characterisation to assess both OM bioaccessibility and complexity which govern SS biodegradability. CSE fractionates the sludge OM into 5 compartments of decreasing accessibility. First applied on three SS samples with different OM stability, fractionation profiles obtained were in accordance with the latter. 3D fluorescence spectroscopy revealed that the bioaccessible compartments were mainly constituted of simple and easily biodegradable OM while the unaccessible ones were largely made of complex and refractory OM. Then, primary, secondary and anaerobically digested sludge with different biodegradabilities were tested. Complexity revealed by 3D fluorescence spectroscopy was linked with biodegradability and chemical accessibility was correlated with sludge bioaccessibility.
authors.affiliation: INRA, UMR 1222, Ecologie microbienne et biogéochimie du sol, 2 Place Pierre Viala, Bâtiment 12, Montpellier cedex 2 F-34060, France; INRA, UR050, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, Narbonne F-11100, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Muller
authors.affiliation: INRA, UR050, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, Narbonne F-11100, France; Veolia Environnement R&D, Centre de Recherche sur l'Eau, Maisons-Laffitte F-78603, France. Electronic address: julie.jimenez@supagro.inra.fr.
authors.forename: Julie
authors.initials: J
authors.lastname: Jimenez
authors.affiliation: INRA, UR050, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, Narbonne F-11100, France.
authors.forename: Maxime
authors.initials: M
authors.lastname: Antonini
authors.affiliation: INRA, UMR 1222, Ecologie microbienne et biogéochimie du sol, 2 Place Pierre Viala, Bâtiment 12, Montpellier cedex 2 F-34060, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Dudal
authors.affiliation: INRA, UR050, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, Narbonne F-11100, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Latrille
authors.affiliation: Veolia Environnement R&D, Centre de Recherche sur l'Eau, Maisons-Laffitte F-78603, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Vedrenne
authors.affiliation: INRA, UR050, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, Narbonne F-11100, France.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Steyer
authors.affiliation: INRA, UR050, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, Narbonne F-11100, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Patureau
mesh_terms: D001673:Biodegradation, Environmental; D019149:Bioreactors; D005591:Chemical Fractionation / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods*; D009930:Organic Chemicals / Q000032:analysis*; D012722:Sewage / Q000032:analysis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014865:Waste Disposal, Fluid
chemical_list: D009930:Organic Chemicals; D012722:Sewage
keywords: Anaerobic digestion; Bioaccessibility; Biodegradability; Excitation–emission-matrix fluorescence spectroscopy; Organic matter fractionation; Sewage sludge