title: Impact of silk biomaterial structure on proteolysis.
abstract: The goal of this study was to determine the impact of silk biomaterial structure (e.g. solution, hydrogel, film) on proteolytic susceptibility. In vitro enzymatic degradation of silk fibroin hydrogels and films was studied using a variety of proteases, including proteinase K, protease XIV, α-chymotrypsin, collagenase, matrix metalloproteinase-1 (MMP-1) and MMP-2. Hydrogels were used to assess bulk degradation while films were used to assess surface degradation. Weight loss, secondary structure determined by Fourier transform infrared spectroscopy and degradation products analyzed via sodium dodecyl sulfate-polyacrylamide gel electrophoresis were used to evaluate degradation over 5 days. Silk films were significantly degraded by proteinase K, while silk hydrogels were degraded more extensively by protease XIV and proteinase K. Collagenase preferentially degraded the β-sheet content in hydrogels while protease XIV and α-chymotrypsin degraded the amorphous structures. MMP-1 and MMP-2 degraded silk fibroin in solution, resulting in a decrease in peptide fragment sizes over time. The link between primary sequence mapping with protease susceptibility provides insight into the role of secondary structure in impacting proteolytic access by comparing solution vs. solid state proteolytic susceptibility.
authors.affiliation: Department of Biomedical Engineering, School of Engineering, Tufts University, 4 Colby St., Medford, MA 02155, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Brown
authors.affiliation: Department of Biomedical Engineering, School of Engineering, Tufts University, 4 Colby St., Medford, MA 02155, USA.
authors.forename: Chia-Li
authors.initials: CL
authors.lastname: Lu
authors.affiliation: Department of Biomedical Engineering, School of Engineering, Tufts University, 4 Colby St., Medford, MA 02155, USA.
authors.forename: Jeannine
authors.initials: J
authors.lastname: Coburn
authors.affiliation: Department of Biomedical Engineering, School of Engineering, Tufts University, 4 Colby St., Medford, MA 02155, USA. Electronic address: david.kaplan@tufts.edu.
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
mesh_terms: D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D008422:Materials Testing; D008567:Membranes, Artificial; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000737:chemistry*; D011487:Protein Conformation; D059748:Proteolysis; D047011:Silk / Q000737:chemistry* / Q000648:ultrastructure*
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D008567:Membranes, Artificial; D047011:Silk; D010447:Peptide Hydrolases
keywords: Digestion; Films; Hydrogels; Protease; Silk