title: Schizosaccharomyces pombe: a novel transport vehicle of functional DNA and mRNA into mammalian antigen-presenting cells.
abstract: Vaccine vehicles based on recombinant yeasts have become promising candidates for the induction of cellular immune responses. In this study, we investigated the capacity of the fission yeast Sz. pombe for the delivery of functional nucleic acids into murine and human antigen-presenting cells. We demonstrate that Sz. pombe cells effectively induce maturation of human dendritic cells (DC), an important prerequisite for T-cell activation. Further, recombinant fission yeast efficiently delivers functional DNA and mRNA into murine macrophages and human DC resulting in the expression of the model antigen eGFP in these cells. Thus, Sz. pombe suggests itself as a promising candidate for a novel live vaccine.
authors.affiliation: Molecular and Cell Biology, Saarland University, 66123 Saarbrücken, Germany; Junior Research Group for Virology and Immunology, Institute of Virology and Center of Human and Molecular Biology, Medical Faculty, Saarland University, 66421 Homburg Saar, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Walch-Rückheim
authors.affiliation: Molecular and Cell Biology, Saarland University, 66123 Saarbrücken, Germany.
authors.forename: Manfred J
authors.initials: MJ
authors.lastname: Schmitt
authors.affiliation: Molecular and Cell Biology, Saarland University, 66123 Saarbrücken, Germany. Electronic address: fb@microbiol.uni-sb.de.
authors.forename: Frank
authors.initials: F
authors.lastname: Breinig
mesh_terms: D000938:Antigen-Presenting Cells / Q000276:immunology*; D002460:Cell Line; D004247:DNA / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D008264:Macrophages / Q000276:immunology*; D012333:RNA, Messenger / Q000276:immunology; D012568:Schizosaccharomyces
chemical_list: D012333:RNA, Messenger; D004247:DNA
keywords: Schizosaccharomyces pombe; Vaccine; Yeast