title: Mangiferin induces apoptosis by regulating Bcl-2 and Bax expression in the CNE2 nasopharyngeal carcinoma cell line.
abstract: To investigate the anti-proliferative mechanism of mangiferin in a human nasopharyngeal carcinoma cell line, CNE2 cells were incubated with different concentrations of mangiferin (12.5, 25, 50, 100, 150 and 200 μM) or with PBS as a control for 72 hours. Analyses were made of the cell cycle and apoptosis with measurement of mRNA and protein levels of two apoptosis-related genes, Bcl-2 and Bax. Flow cytometry assays showed mangiferin could inhibit CNE2 cell proliferation via G2/M arrest and induction of early apoptosis. Real time PCR and Western blotting showed the mRNA and protein level of Bcl-2 to be down-regulated, while those of Bax were up-regulated, when CNE2 cells were treated with mangiferin. This investigation indicated anti-proliferation effects of mangiferin through induction of cell apoptosis regulated by Bcl-2 and Bax expression.
authors.affiliation: Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning, China E-mail : lingmin70@163.com.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Pan
authors.forename: Ai-Yan
authors.initials: AY
authors.lastname: Wang
authors.forename: Yong-Qi
authors.initials: YQ
authors.lastname: Huang
authors.forename: Yu
authors.initials: Y
authors.lastname: Luo
authors.forename: Min
authors.initials: M
authors.lastname: Ling
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002277:Carcinoma; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D019254:Genes, bcl-2 / Q000187:drug effects; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044004:Xanthones / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D044004:Xanthones; D051028:bcl-2-Associated X Protein; C013592:mangiferin