title: Peroxidase-like activity of apoferritin paired gold clusters for glucose detection.
abstract: The discovery and application of noble metal nanoclusters have received considerable attention. In this paper, we reported that apoferritin paired gold clusters (Au-Ft) could efficiently catalyze oxidation of 3.3',5.5'-tetramethylbenzidine (TMB) by H2O2 to produce a blue color reaction. Compared with natural enzyme, Au-Ft exhibited higher activity near acidic pH and could be used over a wide range of temperatures. Apoferritin nanocage enhanced the reaction activity of substrate TMB by H2O2. The reaction catalyzed by Au-Ft was found to follow a typical Michaelis-Menten kinetics. The kinetic parameters exhibited a lower K(m) value (0.097 mM) and a higher K(cat) value (5.8 × 10(4) s(-1)) for TMB than that of horse radish peroxidase (HRP). Base on these findings, Au-Ft, acting as a peroxidase mimetic, performed enzymatic spectrophotometric analysis of glucose. This system exhibited acceptable reproducibility and high selectivity in biosening, suggesting that it could have promising applications in the future.
authors.affiliation: Key laboratory for Molecular Enzymology and Engineering, the Ministry of Education, Collage of Life Science, Jilin University, Changchun 130012, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Jiang
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, PR China.
authors.forename: Cuiji
authors.initials: C
authors.lastname: Sun
authors.affiliation: Key laboratory for Molecular Enzymology and Engineering, the Ministry of Education, Collage of Life Science, Jilin University, Changchun 130012, PR China. Electronic address: guoyi@jlu.edu.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Guo
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, PR China.
authors.forename: Guangjun
authors.initials: G
authors.lastname: Nie
authors.affiliation: Key laboratory for Molecular Enzymology and Engineering, the Ministry of Education, Collage of Life Science, Jilin University, Changchun 130012, PR China; National Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun 130012, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
mesh_terms: D001052:Apoferritins / Q000737:chemistry*; D040761:Biomimetic Materials / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D053761:Nanocomposites / Q000737:chemistry / Q000648:ultrastructure; D009195:Peroxidase / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D006046:Gold; D001052:Apoferritins; D009195:Peroxidase; D005947:Glucose
keywords: Apoferritin; Glucose; Gold clusters; Kinetic study; Peroxidase-like activity