title: Immunogenicity and protective efficacy of a major White Spot Syndrome Virus (WSSV) envelope protein VP24 expressed in Escherichia coli against WSSV.
abstract: The study reports cloning, expression and characterization of immunogenic activity of VP24, a major envelope protein of White Spot Syndrome Virus (WSSV). His-tagged VP24 was expressed as truncated protein and purified from inclusion bodies by metal affinity chromatography under denaturing conditions. The ability to confer protection from WSSV by oral administration of recombinant viral protein (rVP24) was examined in black tiger shrimp Penaeus monodon (P. monodon) juveniles (advanced post larvae). Animals were fed with rVP24 for 10 days, orally challenged with WSSV and assayed for expression of viral genes and shrimp immune genes on the 2nd, 5th and 8th days of challenge. The survival of juvenile shrimps in the vaccinated and challenged group was significantly higher compared to the unvaccinated and challenged group with lesser viral gene expression (DNA polymerase, latency 1 and vp28). Analysis of immune gene expression showed upregulation of syntenin and down regulation of STAT, Rab 7 and caspase during the experimental period. This study points to the feasibility of using rVP24 as candidate vaccine in P. monodon against WSSV.
authors.affiliation: Centre for Biotechnology, Anna University, Chennai, India. Electronic address: anci.thomas@gmail.com.
authors.forename: Ancy
authors.initials: A
authors.lastname: Thomas
authors.affiliation: National Center for Aquatic Animal Health, Cochin University of Science and Technology, Cochin, India. Electronic address: cusatmarine@gmail.com.
authors.forename: Naduvilamuriparampu Saidumuhammed
authors.initials: NS
authors.lastname: Sudheer
authors.affiliation: Centre for Biotechnology, Anna University, Chennai, India. Electronic address: karthikbio.v@gmail.com.
authors.forename: Karthik
authors.initials: K
authors.lastname: Viswanathan
authors.affiliation: Faculty of Biosciences and Aquaculture, University of Nordland, 8049 Bodø, Norway. Electronic address: kiron.viswanath@hibo.no.
authors.forename: Viswanath
authors.initials: V
authors.lastname: Kiron
authors.affiliation: National Center for Aquatic Animal Health, Cochin University of Science and Technology, Cochin, India. Electronic address: isbsingh@gmail.com.
authors.forename: Issac S
authors.initials: IS
authors.lastname: Bright Singh
authors.affiliation: Centre for Biotechnology, Anna University, Chennai, India. Electronic address: rbn@annauniv.edu.
authors.forename: Rangarajan Badri
authors.initials: RB
authors.lastname: Narayanan
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004926:Escherichia coli; D033561:Penaeidae / Q000276:immunology / Q000821:virology*; D011994:Recombinant Proteins / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*; D046848:White spot syndrome virus 1
chemical_list: D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines
keywords: Shrimp immune response; Shrimp vaccine; VP24; WSSV