title: Locus coeruleus lesions and PCOS: role of the central and peripheral sympathetic nervous system in the ovarian function of rat.
abstract: BACKGROUND
  "Polycystic ovary syndrome (PCOS) is a complex endocrine and metabolic disorder associated with ovulatory dysfunction". "Autonomic and central nervous systems play important roles in the regulation of ovarian physiology". The noradrenergic nucleus locus coeruleus (LC) plays a central role in the regulation of the sympathetic nervous system and synaptically connected to the preganglionic cell bodies of the ovarian sympathetic pathway and its activation is essential to trigger spontaneous or induced LH surges. This study evaluates sympathetic outflow in central and peripheral pathways in PCO rats.
  
  
  OBJECTIVE
  Our objectives in this study were (1) to estimate LC activity in rats with estradiol valerate (EV)-induced PCO; (2) to antagonized alpha2a adrenoceptor in systemic conditions with yohimbine.
  
  
  METHODS
  Forty two rats were divided into two groups: 1) LC and yohimbine and 2) control. Every group subdivided in two groups: eighteen rats were treated with estradiol valerate for induction of follicular cysts and the remainders were sesame oil groups.
  
  
  RESULTS
  Estradiol concentration was significantly augmented by the LC lesion in PCO rats (p<0.001), while LC lesion could not alter serum concentrations of LH and FSH, like yohimbine. The morphological observations of ovaries of LC lesion rats showed follicles with hyperthecosis, but yohimbine reduced the number of cysts, increased corpus lutea and developed follicles.
  
  
  CONCLUSIONS
  Rats with EV-induced PCO increased sympathetic activity. LC lesion and yohimbine decreased the number of cysts and yohimbine increased corpus lutea and developed follicles in PCO rats.
authors.affiliation: Vali-e-Asr Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Farideh
authors.initials: F
authors.lastname: Zafari Zangeneh
authors.affiliation: Vali-e-Asr Hospital, Pathology Laboratory, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Abdollahi
authors.affiliation: Veterinary Faculty, Tehran University, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Aminee
authors.affiliation: Central Biostatistics, Fasa University of Medical Sciences, Fasa, Iran.
authors.forename: Mohammad Mahdi
authors.initials: MM
authors.lastname: Naghizadeh
keywords: Estradiol; Gonadotropines; Locus coeruleus; Ovary morphology; Polycystic ovary syndrome; Rat