title: Bioprocessing of some agro-industrial residues for endoglucanase production by the new subsp.; Streptomyces albogriseolus subsp. cellulolyticus strain NEAE-J.
abstract: The use of low cost agro-industrial residues for the production of industrial enzymes is one of the ways to reduce significantly production costs. Cellulase producing actinomycetes were isolated from soil and decayed agricultural wastes. Among them, a potential culture, strain NEAE-J, was selected and identified on the basis of morphological, cultural, physiological and chemotaxonomic properties, together with 16S rDNA sequence. It is proposed that strain NEAE-J should be included in the species Streptomyces albogriseolus as a representative of a novel sub-species, Streptomyces albogriseolus subsp. cellulolyticus strain NEAE-J and sequencing product was deposited in the GenBank database under accession number JN229412. This organism was tested for its ability to produce endoglucanase and release reducing sugars from agro-industrial residues as substrates. Sugarcane bagasse was the most suitable substrate for endoglucanase production. Effects of process variables, namely incubation time, temperature, initial pH and nitrogen source on production of endoglucanase by submerged fermentation using Streptomyces albogriseolus subsp. cellulolyticus have been studied. Accordingly optimum conditions have been determined. Incubation temperature of 30 °C after 6 days, pH of 6.5, 1% sugarcane bagasse as carbon source and peptone as nitrogen source were found to be the optimum for endoglucanase production. Optimization of the process parameters resulted in about 2.6 fold increase in the endoglucanase activity. Therefore, Streptomyces albogriseolus subsp. cellulolyticus coud be potential microorganism for the intended application.
authors.affiliation: Department of Bioprocess Development Genetic Engineering and Biotechnology Research Institute City of Scientific Research and Technological ApplicationsAlexandria Egypt Department of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Alexandria, Egypt.
authors.forename: Noura El-Ahmady
authors.initials: Nel-A
authors.lastname: El-Naggar
authors.affiliation: Chemistry of Natural and Microbial Products Department Pharmaceutical Industry Division National Research Center DokkiCairo Egypt Chemistry of Natural and Microbial Products Department, Pharmaceutical Industry Division, National Research Center, Dokki, Cairo, Egypt.
authors.forename: Nayera A M
authors.initials: NA
authors.lastname: Abdelwahed
authors.affiliation: Microbial Activity Unit Microbiology Department Soils, Water and Environment Research Institute Agricultural Research Center Giza Egypt Microbial Activity Unit, Microbiology Department, Soils, Water and Environment Research Institute, Agricultural Research Center, Giza, Egypt.
authors.forename: Wesam I A
authors.initials: WI
authors.lastname: Saber
authors.affiliation: Chemistry of Natural and Microbial Products Department Pharmaceutical Industry Division National Research Center DokkiCairo Egypt Chemistry of Natural and Microbial Products Department, Pharmaceutical Industry Division, National Research Center, Dokki, Cairo, Egypt.
authors.forename: Asem A
authors.initials: AA
authors.lastname: Mohamed
mesh_terms: D015373:Bacterial Typing Techniques; D002241:Carbohydrates / Q000032:analysis; D002480:Cellulase / Q000302:isolation & purification* / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D031786:Saccharum / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
chemical_list: D002241:Carbohydrates; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002482:Cellulose; C027433:bagasse; D002480:Cellulase
keywords: 16S rRNA; Streptomyces albogriseolus; agro-industrial residues; endoglucanase production; scanning electron microscope