title: Ethylene response factor Sl-ERF.B.3 is responsive to abiotic stresses and mediates salt and cold stress response regulation in tomato.
abstract: Sl-ERF.B.3 (Solanum lycopersicum ethylene response factor B.3) gene encodes for a tomato transcription factor of the ERF (ethylene responsive factor) family. Our results of real-time RT-PCR showed that Sl-ERF.B.3 is an abiotic stress responsive gene, which is induced by cold, heat, and flooding, but downregulated by salinity and drought. To get more insight into the role of Sl-ERF.B.3 in plant response to separate salinity and cold, a comparative study between wild type and two Sl-ERF.B.3 antisense transgenic tomato lines was achieved. Compared with wild type, Sl-ERF.B.3 antisense transgenic plants exhibited a salt stress dependent growth inhibition. This inhibition was significantly enhanced in shoots but reduced in roots, leading to an increased root to shoot ratio. Furthermore, the cold stress essay clearly revealed that introducing antisense Sl-ERF.B.3 in transgenic tomato plants reduces their cell injury and enhances their tolerance against 14 d of cold stress. All these results suggest that Sl-ERF.B.3 gene is involved in plant response to abiotic stresses and may play a role in the layout of stress symptoms under cold stress and in growth regulation under salinity.
authors.affiliation: Laboratoire de Morphogenèse et de Biotechnologie Végétale, Faculté des Sciences de Tunis (FST), Campus Universitaire 2092 El Manar Tunis, Tunisia ; Université Toulouse, INP ENSA Toulouse, 31326 Castanet-Tolosan, France ; INRA, 31326 Castanet-Tolosan, France.
authors.forename: Imen
authors.initials: I
authors.lastname: Klay
authors.affiliation: Université Toulouse, INP ENSA Toulouse, 31326 Castanet-Tolosan, France ; INRA, 31326 Castanet-Tolosan, France.
authors.forename: Julien
authors.identifier: 0000-0002-5725-885X
authors.initials: J
authors.lastname: Pirrello
authors.affiliation: LR Biotechnologie et Valorisation des Bio-Géo Ressources (LR11ES31), Institut Supérieur de Biotechnologie, Université de La Manouba Biotech Pole de Sidi Thabet, Sidi Thabet, 2020 Ariana, Tunisia.
authors.forename: Leila
authors.initials: L
authors.lastname: Riahi
authors.affiliation: Université Toulouse, INP ENSA Toulouse, 31326 Castanet-Tolosan, France ; INRA, 31326 Castanet-Tolosan, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Bernadac
authors.affiliation: LR Biotechnologie et Valorisation des Bio-Géo Ressources (LR11ES31), Institut Supérieur de Biotechnologie, Université de La Manouba Biotech Pole de Sidi Thabet, Sidi Thabet, 2020 Ariana, Tunisia.
authors.forename: Ameur
authors.identifier: 0000-0001-7310-3842
authors.initials: A
authors.lastname: Cherif
authors.affiliation: Université Toulouse, INP ENSA Toulouse, 31326 Castanet-Tolosan, France ; INRA, 31326 Castanet-Tolosan, France.
authors.forename: Mondher
authors.initials: M
authors.lastname: Bouzayen
authors.affiliation: Laboratoire de Morphogenèse et de Biotechnologie Végétale, Faculté des Sciences de Tunis (FST), Campus Universitaire 2092 El Manar Tunis, Tunisia.
authors.forename: Sadok
authors.initials: S
authors.lastname: Bouzid
mesh_terms: D000595:Amino Acid Sequence; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics; D016415:Sequence Alignment; D013312:Stress, Physiological / Q000235:genetics*; D013696:Temperature; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors