title: Carbodimide cross-linked and biodegradation-controllable small intestinal submucosa sheets.
abstract: The small intestinal submucosa (SIS) is an acellular collagen-based matrix, primarily composed of fibrillar collagens (types I, II, and V). They enhance healing due to a minimal immune response. A good degradation rate is the degradation of materials equal to the rate of remodeling in the host. The SIS should apply a good degradation rate and cytocompatibility. In this study, a series of SIS with different degradation rates is obtained by treatment with Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). Morphology, composition, degradable ratio, mechanical properties and cytocompatibility of the SIS are evaluated. We determined a 20 μm thickness and 60 μm pore size of the native SIS. The degradable ratio of the native SIS was approximately 90% in the presence of 0.25 mg/ml collagenase for 24 hours. The storage modulus of the native SIS was 388 MPa. The degradable ratio of the SIS decreased to 6% and the storage modulus increased to 777 MPa after being treated with 30 mM EDC for 24 hours. In cytocompatibility assay, cell numbers on the native SIS were similar as on the treated SIS due to the non-toxicity of the EDC treatment process. This SIS exhibited collagenase resistance, stronger mechanical strength and good cytocompatibility after the EDC treatment concluded. The cross-linked SIS could be utilized as a potential cell carrier for tissue engineering application.
authors.affiliation: Department of Biomedical Engineering, Ming-Chuan University, 5 De Ming Rd., Gui Shan District, Taoyuan, 333, Taiwan Metal Industries Research & Development Centre, 6F, No. 162-24, Sec. 3, Hsin-Yi Rd., Taipei 10658, Taiwan.
authors.forename: Ching-Cheng
authors.initials: CC
authors.lastname: Huang
authors.affiliation: Department of Life Science, Fu Jen Catholic University, No. 510, Jhongiheng Rd., Sinjhuang Dist., New Taipei City 24205, Taiwan.
authors.forename: Ching-Yi
authors.initials: CY
authors.lastname: Liu
authors.affiliation: Department of Life Science, Fu Jen Catholic University, No. 510, Jhongiheng Rd., Sinjhuang Dist., New Taipei City 24205, Taiwan.
authors.forename: Chi-Yen
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Department of Life Science, Fu Jen Catholic University, No. 510, Jhongiheng Rd., Sinjhuang Dist., New Taipei City 24205, Taiwan.
authors.forename: Hsia-Wei
authors.initials: HW
authors.lastname: Liu
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D002234:Carbodiimides / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D003432:Cross-Linking Reagents / Q000737:chemistry; D019544:Equipment Failure Analysis; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D007413:Intestinal Mucosa / Q000737:chemistry* / Q000166:cytology*; D008422:Materials Testing; D051379:Mice; D011474:Prosthesis Design; D011817:Rabbits; D013552:Swine; D013718:Tensile Strength; D054457:Tissue Scaffolds
chemical_list: D002234:Carbodiimides; D003432:Cross-Linking Reagents
keywords: Carbodiimide cross-linked; biodegradation; cytocompatibility; small intestinal submucosa (SIS)