title: Adhesion of indigenous Lactobacillus plantarum to gut extracellular matrix and its physicochemical characterization.
abstract: Adhesion to the human intestinal epithelial cell is considered as one of the important selection criteria of lactobacilli for probiotic attributes. Sixteen Lactobacillus plantarum strains from human origins were subjected for adhesion to extracellular matrix (ECM) components, and their physiochemical characterization, incubation time course and effect of different pH on bacterial adhesion in vitro were studied. Four strains showed significant binding to both fibronectin and mucin. After pretreatment with pepsin and trypsin, the bacterial adhesion to ECM reduced to the level of 50 % and with lysozyme significantly decreased by 65-70 %. Treatment with LiCl also strongly inhibited (90 %) the bacterial adhesion to ECM. Tested strains showed highest binding efficacy at time course of 120 and 180 min. Additionally, the binding of Lp91 to ECM was highest at pH 6 (155 ± 2.90 CFU/well). This study proved that surface layer components are proteinaceous in nature, which contributed in adhesion of lactobacillus strains. Further, the study can provide a better platform for introduction of new indigenous probiotic strains having strong adhesion potential for future use.
authors.affiliation: Department of Microbiology and Immunology, National Institute of Nutrition, Hyderabad, India, ashok.ndri@gmail.com.
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Yadav
authors.forename: Ashish
authors.initials: A
authors.lastname: Tyagi
authors.forename: Ashwani
authors.initials: A
authors.lastname: Kumar
authors.forename: Asha Chandola
authors.initials: AC
authors.lastname: Saklani
authors.forename: Sunita
authors.initials: S
authors.lastname: Grover
authors.forename: Virender Kumar
authors.initials: VK
authors.lastname: Batish
mesh_terms: D001422:Bacterial Adhesion; D004847:Epithelial Cells / Q000382:microbiology; D005109:Extracellular Matrix / Q000737:chemistry / Q000382:microbiology*; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000382:microbiology; D048191:Lactiplantibacillus plantarum / Q000378:metabolism / Q000502:physiology*; D009077:Mucins / Q000378:metabolism; D019936:Probiotics
chemical_list: D005353:Fibronectins; D009077:Mucins