title: Effect of chronic Sildenafil treatment on the prostate of C57Bl/6 mice.
abstract: Sildenafil is a potent and selective inhibitor of phosphodiesterase-5 (PDE5) and is considered first-line therapy for erectile dysfunction. Nowadays, Sildenafil is used extensively throughout the world on patients with pulmonary hypertension. However, few studies have evaluated the possible side effects of chronic Sildenafil treatment on the male reproductive system, specifically in the prostate. In the present study, it was demonstrated via morphological and ultrastructural analysis that chronic treatment with Sildenafil induced an enhancement of the glandular activity of the prostate. In addition, mice treated with Sildenafil showed a significant increase in testosterone serum levels. However, no statistically significant differences were observed in nitric oxide serum levels, or in sGC, eNOS, PSA and TGF-β prostatic expression. In conclusion, the present study suggests that chronic use of Sildenafil does not cause evident prostatic damage, and therefore, can be used pharmacologically to treat a variety of disorders.
authors.affiliation: Laboratório de Ultraestrutura do Instituto Aggeu Magalhães (FIOCRUZ), Brazil; Universidade Federal de Pernambuco (UFPE), Brazil. Electronic address: gomes.bio@gmail.com.
authors.forename: Fabiana Oliveira dos Santos
authors.initials: FO
authors.lastname: Gomes
authors.affiliation: Laboratório de Microscopia e Microanálise do Centro de Tecnologias Estratégicas do Nordeste (CETENE), Brazil.
authors.forename: Maria da Conceição
authors.initials: Mda C
authors.lastname: Carvalho
authors.affiliation: Departamento de Biologia da Universidade Estadual da Paraíba, Campina Grande, Brazil.
authors.forename: Karina Lidianne Alcântara
authors.initials: KL
authors.lastname: Saraiva
authors.affiliation: Laboratório de Ultraestrutura do Instituto Aggeu Magalhães (FIOCRUZ), Brazil; Universidade Federal de Pernambuco (UFPE), Brazil.
authors.forename: Edlene Lima
authors.initials: EL
authors.lastname: Ribeiro
authors.affiliation: Laboratório de Ultraestrutura do Instituto Aggeu Magalhães (FIOCRUZ), Brazil; Universidade Federal de Pernambuco (UFPE), Brazil.
authors.forename: Amanda Karolina Soares
authors.initials: AK
authors.lastname: E Silva
authors.affiliation: Laboratório de Ultraestrutura do Instituto Aggeu Magalhães (FIOCRUZ), Brazil; Universidade Federal de Pernambuco (UFPE), Brazil.
authors.forename: Mariana Aragão Matos
authors.initials: MA
authors.lastname: Donato
authors.affiliation: Laboratório de Ultraestrutura do Instituto Aggeu Magalhães (FIOCRUZ), Brazil; Universidade Federal de Pernambuco (UFPE), Brazil.
authors.forename: Sura Wanessa Santos
authors.initials: SW
authors.lastname: Rocha
authors.affiliation: Laboratório de Ultraestrutura do Instituto Aggeu Magalhães (FIOCRUZ), Brazil; Universidade Federal de Pernambuco (UFPE), Brazil.
authors.forename: Bruna
authors.initials: B
authors.lastname: Santos e Silva
authors.affiliation: Laboratório de Ultraestrutura do Instituto Aggeu Magalhães (FIOCRUZ), Brazil.
authors.forename: Christina Alves
authors.initials: CA
authors.lastname: Peixoto
mesh_terms: D000818:Animals; D007172:Erectile Dysfunction / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D006976:Hypertension, Pulmonary / Q000188:drug therapy / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000097:blood; D052250:Nitric Oxide Synthase Type III / Q000097:blood; D010879:Piperazines / Q000008:administration & dosage*; D011467:Prostate / Q000187:drug effects / Q000648:ultrastructure*; D017430:Prostate-Specific Antigen / Q000097:blood; D011687:Purines / Q000008:administration & dosage; D000068677:Sildenafil Citrate; D013449:Sulfonamides / Q000008:administration & dosage*; D013739:Testosterone / Q000097:blood; D016212:Transforming Growth Factor beta / Q000097:blood
chemical_list: D010879:Piperazines; D011687:Purines; D013449:Sulfonamides; D016212:Transforming Growth Factor beta; D009569:Nitric Oxide; D013739:Testosterone; D000068677:Sildenafil Citrate; D052250:Nitric Oxide Synthase Type III; C496315:Nos3 protein, mouse; D017430:Prostate-Specific Antigen
keywords: Inhibitor of phosphodiesterase-5 (PDE5); Prostate; Sildenafil