title: Interleukin-1β-31C/T and -511T/C polymorphisms were associated with preeclampsia in Chinese Han population.
abstract: OBJECTIVE
  The purpose of our study is to investigate the relationship between IL-1β -31C/T (rs1143627) and -511T/C (rs16944) polymorphisms and the preeclampsia (PE), and analyze the Linkage disequilibrium (LD) and haplotype frequency of the two polymorphism loci.
  
  
  METHODS
  Polymorphisms at -31C/T and -511T/C of IL-1β were genotyped with the method of polymerase chain reaction-restriction fragment length polymorphism (PCR- RFLP) in 232 PE and 447 control subjects. Genotype and allele frequencies between case-control groups were compared by chi-square(X2) tests. Two-point LD and haplotype frequency analyses were done with the software Haploview4.2.
  
  
  RESULTS
  Significant statistical differences were found between PE and control groups regarding genotype and allele frequencies of the two polymorphisms of IL-1β (For IL-1β -31C/T: X2 = 11.478, P = 0.003; For IL-1β-511T/C: X2 = 9.687, P = 0.008). LD analysis revealed that the IL-1β -31C/T SNP was in high LD with the IL-1β-511C/T SNP(D' = 0.92, r2 = 0.79). Both CT and TC haplotypes showed significant differences between case and control groups. Only the plasma level of Prothrombin Time had a significantly statistical difference among TT, CT and CC groups of the preeclamptic two polymorphisms of IL-1β-31C/T and -511T/C (for IL-1β-31C/T, F = 1.644, P = 0.01; F = 1.587, P = 0.016).
  
  
  CONCLUSIONS
  Our results revealed IL-1β was associated with the PE in Chinese Han population. The CT haplotype may increase the risk of PE, while haplotype TC could be considered as a protective haplotype of PE.
authors.affiliation: Prenatal diagnosis center, the Affiliated Hospital of Qingdao University, Qingdao, China; Shandong Provincial Key Laboratory of Metabolic Disease, the Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of clinical laboratory diagnosis, Medical College, Qingdao University, Qingdao, China.
authors.forename: Fengli
authors.initials: F
authors.lastname: Jiang
authors.affiliation: Department of Interventional radiology, the Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Respiratory Medicine, Heze Medical College, Heze, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Interventional radiology, The Songshan Hospital of Medical College, Qingdao University, Qingdao, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Li
authors.affiliation: Internal medicine, The Qingdao Fuwai Cardiovascular Hospital, Qingdao, Qingdao, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Prenatal diagnosis center, the Affiliated Hospital of Qingdao University, Qingdao, China; Shandong Provincial Key Laboratory of Metabolic Disease, the Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Shiguo
authors.initials: S
authors.lastname: Liu
authors.affiliation: Prenatal diagnosis center, the Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Yuanhua
authors.initials: Y
authors.lastname: Ye
mesh_terms: D002681:China; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics*; D015810:Linkage Disequilibrium; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy
chemical_list: D053583:Interleukin-1beta