title: Pedobacter rivuli sp. nov., isolated from a freshwater stream.
abstract: A Gram-staining-negative, rod-shaped, red strain, HME8457(T), was isolated from a freshwater stream located in the Republic of Korea. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HME8457(T) formed a lineage within the genus Pedobacter. Strain HME8457(T) was most closely related to species of the genus Pedobacter: Pedobacter daechungensis Dae 13(T) (96.4 % sequence similarity), Pedobacter lentus DS-40(T) (95.3 %), Pedobacter terricola DS-45(T) (94.9 %), Pedobacter glucosidilyticus 1-2(T) (94.2 %) and 'Pedobacter soyangensis' HME6451 (93.6 %). The major fatty acids were iso-C15 : 0 (28.8 %), summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c; 21.7 %), iso-C17 : 0 3-OH (7.7 %) and anteiso-C15 : 0 (6.2 %). The only respiratory quinone was MK-7. Polar lipid analysis revealed the presence of phosphatidylethanolamine, one unidentified aminolipid and two unidentified polar lipids. Sphingolipid was present. The DNA G+C content was 33.3 mol%. On the basis of the evidence presented in this study, strain HME8457(T) represents a novel species of the genus Pedobacter, for which the name Pedobacter rivuli sp. nov. is proposed; the type strain is HME8457(T) ( = KACC 17312(T) = CECT 8291(T)).
authors.affiliation: Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 449-791, Republic of Korea.
authors.forename: Heeyoung
authors.initials: H
authors.lastname: Kang
authors.affiliation: Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 449-791, Republic of Korea.
authors.forename: Haneul
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 449-791, Republic of Korea.
authors.forename: Yochan
authors.initials: Y
authors.lastname: Joung
authors.affiliation: Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 449-791, Republic of Korea.
authors.forename: Kiseong
authors.initials: K
authors.lastname: Joh
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D060485:Pedobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D045483:Rivers / Q000737:chemistry*; D017422:Sequence Analysis, DNA; D013107:Sphingolipids / Q000737:chemistry; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D013107:Sphingolipids; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7