title: Evaluation of an indirect ELISA using recombinant granule antigen Gra7 for serodiagnosis of Toxoplasma gondii infection in cats.
abstract: The precise detection of Toxoplasma gondii infection in cats has important public health significance. In the present study, recombinant granule antigen protein GRA7 was evaluated as a potential diagnostic marker for T. gondii infection in cats by an indirect enzyme-linked immunosorbent assay (ELISA), using the classified serum samples from cats, by immunofluorescence assay (IFA) and by modified agglutination test (MAT). There was a perfect agreement (97.2%) between GRA7-ELISA and MAT/IFA (Kappa = 0.92; 95% confidence interval [CI], 0.85 to 0.99), and GRA7-ELISA had a sensitivity of 94.9% and a specificity of 97.9%. No significant difference (P > 0.05) was observed between the detection results by GRA7- and Toxoplasma lysate antigen-based ELISA. Receiver operating characteristic analysis showed a relative sensitivity and specificity of 89.7 and 92.5% at the cut-off value of 0.1 for GRA7-ELISA. These data demonstrate that GRA7 is a promising serodiagnostic marker for T. gondii infection in cats.
authors.affiliation: College of Life Science, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin Province 130118, PR China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Cai
authors.forename: Zedong
authors.initials: Z
authors.lastname: Wang
authors.forename: Jiping
authors.initials: J
authors.lastname: Li
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Wei
authors.forename: Quan
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology; D015415:Biomarkers; D002371:Cat Diseases / Q000175:diagnosis* / Q000469:parasitology; D002415:Cats; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards / Q000662:veterinary*; D005260:Female; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology; D012372:ROC Curve; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015415:Biomarkers; C112573:GRA7 protein, Toxoplasma gondii; D007074:Immunoglobulin G; D015800:Protozoan Proteins; D011994:Recombinant Proteins