title: Flaxseed lignan wound healing formulation: characterization and in vivo therapeutic evaluation.
abstract: Flaxseed lignans are a natural source of useful biologically active components that show a diverse spectrum of health-promoting properties. The valuable effects of the phenolic molecules are mainly due to their antioxidant activity by preventing oxidative stress and stimulate collagen synthesis, therefore, providing benefits to the skin. The present work highlights the development of flaxseed extract formulation as novel wound healing agent. The recognition of key structural features within flaxseed extract was crucial for the design and development of the therapeutic cream. Chromatographic analyses were employed for bioactive compounds identification and quantification. Folin-Ciocalteu method determined the total phenolic content and the antioxidant properties were evaluated by DPPH assay. The storage and loss modulus and tan δ were calculated for cream rheological properties evaluation. In vitro diffusion capacity and in vivo wound healing activity of phenolic cream were evaluated on Wistar rats. The collective properties and healing effect of the flaxseed suggested wound healing capacity.
authors.affiliation: Faculty of Chemical Engineering and Environmental Protection, "Gheorghe Asachi" Technical University of Iasi, 700050 Iasi, Romania; Institute of Research and Development in Technical and Natural Sciences, "Aurel Vlaicu" University, 310330 Arad, Romania.
authors.forename: D
authors.initials: D
authors.lastname: Draganescu
authors.affiliation: Faculty of Chemical Engineering and Environmental Protection, "Gheorghe Asachi" Technical University of Iasi, 700050 Iasi, Romania.
authors.forename: C
authors.initials: C
authors.lastname: Ibanescu
authors.affiliation: Faculty of Pharmacy, "Grigore T. Popa" University of Medicine and Pharmacy, 700454 Iasi, Romania.
authors.forename: B I
authors.initials: BI
authors.lastname: Tamba
authors.affiliation: Faculty of Chemical Engineering and Environmental Protection, "Gheorghe Asachi" Technical University of Iasi, 700050 Iasi, Romania.
authors.forename: C V
authors.initials: CV
authors.lastname: Andritoiu
authors.affiliation: Faculty of Chemical Engineering and Environmental Protection, "Gheorghe Asachi" Technical University of Iasi, 700050 Iasi, Romania; SCIENT - Research Center for Instrumental Analysis, 060114 Bucharest, Romania.
authors.forename: G
authors.initials: G
authors.lastname: Dodi
authors.affiliation: Faculty of Chemical Engineering and Environmental Protection, "Gheorghe Asachi" Technical University of Iasi, 700050 Iasi, Romania. Electronic address: impopa@yahoo.fr.
authors.forename: M I
authors.initials: MI
authors.lastname: Popa
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry; D002626:Chemistry, Pharmaceutical; D019597:Flax / Q000737:chemistry; D017705:Lignans / Q000008:administration & dosage* / Q000737:chemistry; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000008:administration & dosage / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry; D051381:Rats; D012212:Rheology; D012867:Skin / Q000187:drug effects / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D017705:Lignans; D010636:Phenols; D010936:Plant Extracts; C011471:folin
keywords: DPPH; Flaxseed; In vitro; In vivo; Lignan; Wound healing