title: Upstream regulatory regions controlling the expression of the Candida utilis maltase gene.
abstract: Candida utilis represents a promising expression host, generating relatively high levels of recombinant proteins. The current study presents preliminary characterization of the upstream regulatory regions controlling the carbon source-dependent expression of the C. utilis maltase gene. Cellobiose and soluble starch were recognised as inducers of maltase promoter, besides maltose. We successfully applied the Cre-loxP system to acquire a null mutant strain with disrupted maltase gene and promoter in polyploid yeast C. utilis. Furthermore, the strength and minimal functional region of the promoter was evaluated by measuring β-galactosidase activity. Our results suggest, the qPCR was shown itself a very smart method for relatively easy characterization of the transformants and correlation of the expression levels with gene dosage.
authors.affiliation: Comenius University in Bratislava, Faculty of Natural Sciences, Department of Molecular Biology, Mlynská dolina, 842 15 Bratislava 4, Slovak Republic. Electronic address: halaszovah@fns.uniba.sk.
authors.forename: Hana
authors.initials: H
authors.lastname: Boňková
authors.affiliation: Comenius University in Bratislava, Faculty of Natural Sciences, Department of Molecular Biology, Mlynská dolina, 842 15 Bratislava 4, Slovak Republic. Electronic address: osadska@fns.uniba.sk.
authors.forename: Michaela
authors.initials: M
authors.lastname: Osadská
authors.affiliation: Comenius University in Bratislava, Faculty of Natural Sciences, Department of Molecular Biology, Mlynská dolina, 842 15 Bratislava 4, Slovak Republic. Electronic address: krahulec@nic.fns.uniba.sk.
authors.forename: Ján
authors.initials: J
authors.lastname: Krahulec
authors.affiliation: Comenius University in Bratislava, Faculty of Natural Sciences, Department of Molecular Biology, Mlynská dolina, 842 15 Bratislava 4, Slovak Republic. Electronic address: liskova.veronikaa@gmail.com.
authors.forename: Veronika
authors.initials: V
authors.lastname: Lišková
authors.affiliation: Comenius University in Bratislava, Faculty of Natural Sciences, Department of Molecular Biology, Mlynská dolina, 842 15 Bratislava 4, Slovak Republic. Electronic address: stuchlik@fns.uniba.sk.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Stuchlík
authors.affiliation: Comenius University in Bratislava, Faculty of Natural Sciences, Department of Molecular Biology, Mlynská dolina, 842 15 Bratislava 4, Slovak Republic. Electronic address: turna@fns.uniba.sk.
authors.forename: Ján
authors.initials: J
authors.lastname: Turňa
mesh_terms: D002175:Candida / Q000201:enzymology / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D000520:alpha-Glucosidases / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D000520:alpha-Glucosidases; D001616:beta-Galactosidase
keywords: Candida utilis; Cre-loxP system; Maltase promoter; qPCR; β-Galactosidase