title: In situ hybridization freeze-assisted punches (IFAP): technique for liquid-based tissue extraction from thin slide-mounted sections for DNA methylation analysis.
abstract: In situ hybridization-assisted punches (IFAP) are a low-cost method for extracting tissue from frozen slide-mounted sections as thin as 12 μm. The method synergizes well with standard histological workflows and uses in situ hybridization to target corresponding slide-mounted cryosections that contain the region of interest. Liquid beads of M-1 embedding matrix are applied and snap frozen, binding the matrix to the underlying tissue. Bead-tissue complexes are removed and DNA extracted using a high-salt method. IFAP-extracted DNA is suitable for downstream DNA methylation analysis.
authors.affiliation: Molecular and Behavioral Neuroscience Institute, University of Michigan, 205 Zina Pitcher Place, Biomedical Science Research Building, Room 5698, Ann Arbor, MI, 48109, USA, lsown@voxa.co.
authors.forename: Lawrence S
authors.initials: LS
authors.lastname: Own
authors.forename: Paresh D
authors.initials: PD
authors.lastname: Patel
mesh_terms: D004247:DNA / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D019175:DNA Methylation; D005629:Frozen Sections / Q000379:methods*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D042282:Microdissection / Q000379:methods
chemical_list: D004247:DNA