title: Identification of apolipoprotein C-I as a potential Wilms' tumor marker after excluding inflammatory factors.
abstract: Wilms' tumor is one of the most common malignant tumors observed in children, and its early diagnosis is important for late-stage treatment and prognosis. We previously screened and identified protein markers for Wilms' tumor; however, these markers lacked specificity, and some were associated with inflammation. In the current study, serum samples from children with Wilms' tumors were compared with those of healthy controls and patients with systemic inflammatory response syndrome (SIRS). After exclusion of factors associated with inflammation, specific protein markers for Wilms' tumors were identified. After comparing the protein peak values obtained from all three groups, a protein with a m/z of 6438 Da was specified. Purification and identification of the target protein using high-pressure liquid chromatography (HPLC) and two-dimensional liquid chromatography-linearion trap mass spectrometry(2D-LC-LTQ-MS) mass spectrometry, respectively, revealed that it was apolipoprotein C-I (APO C-I). Thus, APO C-I is a specific protein marker for Wilms' tumor.
authors.affiliation: Department of Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan, China. 13676993275@163.com.
authors.forename: Junjie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan, China. guofei185@163.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.affiliation: Department of Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan, China. wanglei171@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan, China. zhaowei174@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan, China. zhangda191@163.com.
authors.forename: Da
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan, China. yangheying170@163.com.
authors.forename: Heying
authors.initials: H
authors.lastname: Yang
authors.affiliation: Institute of Cancer, the Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310000, Zhejiang, China. yujiekai160@163.com.
authors.forename: Jiekai
authors.initials: J
authors.lastname: Yu
authors.affiliation: Proteomic Platform, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100000, China. niulili163@163.com.
authors.forename: Lili
authors.initials: L
authors.lastname: Niu
authors.affiliation: Proteomic Platform, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100000, China. yangfuquan175@163.com.
authors.forename: Fuquan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Institute of Cancer, the Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310000, Zhejiang, China. zhengshudaniu@163.com.
authors.forename: Shu
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan, China. wjiaxiang175@163.com.
authors.forename: Jiaxiang
authors.initials: J
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D053302:Apolipoprotein C-I / Q000097:blood* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000097:blood* / Q000378:metabolism; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007223:Infant; D007680:Kidney Neoplasms / Q000175:diagnosis*; D008297:Male; D010455:Peptides / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D009396:Wilms Tumor / Q000175:diagnosis*
chemical_list: D053302:Apolipoprotein C-I; D014408:Biomarkers, Tumor; D010455:Peptides