title: Bactericidal activity of nukacin ISK-1: an alternative mode of action.
abstract: We previously reported bacteriostatic action of nukacin ISK-1 against Bacillus subtilis JCM 1465(T). Here, we found its bactericidal activity against Micrococcus luteus DSM 1790 and Staphylococcus simulans 22, showing decrease in cell viability, cell lysis, and dissipation of the membrane potential. Moreover, leakage of small molecules such as K(+), suggested the formation of small-sized or specific K(+)-conducting-pores by nukacin ISK-1.
authors.affiliation: a Laboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture , Graduate School, Kyushu University , Fukuoka , Japan.
authors.forename: Urmi
authors.initials: U
authors.lastname: Roy
authors.forename: Mohammad Riazul
authors.initials: MR
authors.lastname: Islam
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Nagao
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Iida
authors.forename: Abdullah-Al
authors.initials: AA
authors.lastname: Mahin
authors.forename: Mengqi
authors.initials: M
authors.lastname: Li
authors.forename: Takeshi
authors.initials: T
authors.lastname: Zendo
authors.forename: Jiro
authors.initials: J
authors.lastname: Nakayama
authors.forename: Kenji
authors.initials: K
authors.lastname: Sonomoto
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D001430:Bacteriocins / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; C421186:nukacin ISK-1
keywords: antimicrobial peptide; bactericidal activity; lantibiotic; mode of action; unusual amino acid