title: Delayed animal aging through the recovery of stem cell senescence by platelet rich plasma.
abstract: Aging is related to loss of functional stem cell accompanying loss of tissue and organ regeneration potentials. Previously, we demonstrated that the life span of ovariectomy-senescence accelerated mice (OVX-SAMP8) was significantly prolonged and similar to that of the congenic senescence-resistant strain of mice after platelet rich plasma (PRP)/embryonic fibroblast transplantation. The aim of this study is to investigate the potential of PRP for recovering cellular potential from senescence and then delaying animal aging. We first examined whether stem cells would be senescent in aged mice compared to young mice. Primary adipose derived stem cells (ADSCs) and bone marrow derived stem cells (BMSCs) were harvested from young and aged mice, and found that cell senescence was strongly correlated to animal aging. Subsequently, we demonstrated that PRP could recover cell potential from senescence, such as promote cell growth (cell proliferation and colony formation), increase osteogenesis, decrease adipogenesis, restore cell senescence related markers and resist the oxidative stress in stem cells from aged mice. The results also showed that PRP treatment in aged mice could delay mice aging as indicated by survival, body weight and aging phenotypes (behavior and gross morphology) in term of recovering the cellular potential of their stem cells compared to the results on aged control mice. In conclusion these findings showed that PRP has potential to delay aging through the recovery of stem cell senescence and could be used as an alternative medicine for tissue regeneration and future rejuvenation.
authors.affiliation: Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan, ROC; Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei, Taiwan, ROC.
authors.forename: Hen-Yu
authors.initials: HY
authors.lastname: Liu
authors.affiliation: Department of Dentistry, Taipei Medical University Hospital, Taipei, Taiwan, ROC.
authors.forename: Chiung-Fang
authors.initials: CF
authors.lastname: Huang
authors.affiliation: Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan, ROC; Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei, Taiwan, ROC.
authors.forename: Tzu-Chieh
authors.initials: TC
authors.lastname: Lin
authors.affiliation: Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan, ROC; Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei, Taiwan, ROC.
authors.forename: Ching-Yu
authors.initials: CY
authors.lastname: Tsai
authors.affiliation: Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan, ROC.
authors.forename: Szu-Yu
authors.initials: SY
authors.lastname: Tina Chen
authors.affiliation: Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan, ROC; Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei, Taiwan, ROC.
authors.forename: Alice
authors.initials: A
authors.lastname: Liu
authors.affiliation: Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan, ROC; Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei, Taiwan, ROC.
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Chen
authors.affiliation: Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan, ROC; Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei, Taiwan, ROC.
authors.forename: Hong-Jian
authors.initials: HJ
authors.lastname: Wei
authors.affiliation: Department of Food and Nutrition, Providence University, Taichung, Taiwan, ROC.
authors.forename: Ming-Fu
authors.initials: MF
authors.lastname: Wang
authors.affiliation: Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei, Taiwan, ROC; Institute of Regenerative Medicine, Wake Forest School of Medicine, Winston-Salem, NC, USA.
authors.forename: David F
authors.initials: DF
authors.lastname: Williams
authors.affiliation: Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan, ROC; Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei, Taiwan, ROC; Cancer Center, Taipei Medical University Hospital, Taipei, Taiwan, ROC; Translational Research Laboratory, Cancer Center, Taipei Medical University, Taipei, Taiwan, ROC. Electronic address: wpdeng@ms41.hinet.net.
authors.forename: Win-Ping
authors.initials: WP
authors.lastname: Deng
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D000375:Aging; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D016922:Cellular Senescence; D005260:Female; D006801:Humans; D051379:Mice; D010012:Osteogenesis; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
keywords: Adipose derived stem cells (ADSCs); Bone marrow derived stem cells (BMSCs); Delayed aging; Platelet rich plasma (PRP); Senescence