title: A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects.
abstract: The endodermis represents the main barrier to extracellular diffusion in plant roots, and it is central to current models of plant nutrient uptake. Despite this, little is known about the genes setting up this endodermal barrier. In this study, we report the identification and characterization of a strong barrier mutant, schengen3 (sgn3). We observe a surprising ability of the mutant to maintain nutrient homeostasis, but demonstrate a major defect in maintaining sufficient levels of the macronutrient potassium. We show that SGN3/GASSHO1 is a receptor-like kinase that is necessary for localizing CASPARIAN STRIP DOMAIN PROTEINS (CASPs)--major players of endodermal differentiation--into an uninterrupted, ring-like domain. SGN3 appears to localize into a broader band, embedding growing CASP microdomains. The discovery of SGN3 strongly advances our ability to interrogate mechanisms of plant nutrient homeostasis and provides a novel actor for localized microdomain formation at the endodermal plasma membrane.
authors.affiliation: Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Pfister
authors.affiliation: Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Marie
authors.initials: M
authors.lastname: Barberon
authors.affiliation: Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Julien
authors.initials: J
authors.lastname: Alassimone
authors.affiliation: Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Lothar
authors.initials: L
authors.lastname: Kalmbach
authors.affiliation: Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Yuree
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Joop E M
authors.initials: JE
authors.lastname: Vermeer
authors.affiliation: Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Misako
authors.initials: M
authors.lastname: Yamazaki
authors.affiliation: Biochimie et Physiologie Moléculaire des Plantes, CNRS/INRA/SupAgro/Université Montpellier, Montpellier, France.
authors.forename: Guowei
authors.initials: G
authors.lastname: Li
authors.affiliation: Biochimie et Physiologie Moléculaire des Plantes, CNRS/INRA/SupAgro/Université Montpellier, Montpellier, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Maurel
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Hokkaido, Japan.
authors.forename: Junpei
authors.initials: J
authors.lastname: Takano
authors.affiliation: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, United Kingdom.
authors.forename: Takehiro
authors.initials: T
authors.lastname: Kamiya
authors.affiliation: Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, United Kingdom.
authors.forename: David E
authors.initials: DE
authors.lastname: Salt
authors.affiliation: Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Daniele
authors.initials: D
authors.lastname: Roppolo
authors.affiliation: Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Niko
authors.initials: N
authors.lastname: Geldner
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001692:Biological Transport / Q000235:genetics; D000075686:COP9 Signalosome Complex; D002454:Cell Differentiation / Q000235:genetics; D004058:Diffusion; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D006706:Homeostasis / Q000235:genetics*; D008055:Lipids / Q000096:biosynthesis; D018613:Microscopy, Confocal; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D018517:Plant Roots / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011188:Potassium / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014867:Water / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D008055:Lipids; D009687:Nuclear Proteins; D014867:Water; C065875:suberin; D011494:Protein Kinases; D000075686:COP9 Signalosome Complex; D011188:Potassium
keywords: arabidopsis; cell biology; plant biology; plant nutrition; polarity; root biology