title: Emdogain effect on gingival fibroblast adhesion in bioabsorbable and non-resorbable barrier membranes: An in vitro study.
abstract: BACKGROUND
  Tissue engineering represents very exciting advances in regenerative medicine; however, periodontal literature only contains few reports. Emdogain (EMD) consists of functional molecules that have shown many advantages in regenerative treatments. This study investigated EMD effect on gingival fibroblast adhesion to different membranes.
  
  
  METHODS
  Two dense polytetrafluoroethylene membranes (GBR-200, TXT-200), Alloderm and a collagenous membrane (RTM Collagen) were used in this experimental study. Each membrane was cut into four pieces and placed at the bottom of a well in a 48-well plate. 10 μg/mL of EMD was added to two wells of each group. Two wells were left EMD free. Gingival fibroblasts were seeded to all the wells. Cell adhesion was evaluated by means of a Field Emission Scanning Electron Microscope after 24 hours incubation. Data was analyzed by independent t-test, one-way and two-way ANOVA and post hoc LSD test. P < 0.05 in independent t-test analysis and P < 0.001 in one-way ANOVA, two-way ANOVA and post hoc LSD analysis was considered statistically significant.
  
  
  RESULTS
  Alloderm had the highest cell adhesion capacity in EMD+ group and the difference was statistically significant (P < 0.001). In EMD- group, cell adhesion to TXT-200 and Alloderm was significantly higher than GBR-200 and collagenous membrane (P < 0.001).
  
  
  CONCLUSIONS
  This study showed that EMD may decrease the cell adhesion efficacy of GBR-200, TXT-200 and collagenous membrane but it can promote this efficacy in Alloderm. It also showed the composition of biomaterials, their surface textures and internal structures can play an important role in their cell adhesion efficacy.
authors.affiliation: Department of Restorative Dentistry, Khorasgan Islamic Azad Dental School, Ahvaz, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Barekatain
authors.affiliation: Department of Periodontics, Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: Morvarid
authors.initials: M
authors.lastname: Mafi
authors.affiliation: Department of Periodontics, Khorasgan Islamic Azad Dental School, Isfahan, Iran.
authors.forename: Shirin
authors.initials: S
authors.lastname: Amini
authors.affiliation: Department of Periodontics, Khorasgan Islamic Azad Dental School, Isfahan, Iran.
authors.forename: Shirin Zahra
authors.initials: SZ
authors.lastname: Farhad
keywords: Alloderm; cell adhesion; emdogain; guided tissue regeneration; polytetrafluroethylene