title: Scope and limitations of the TEMPO/EPR method for singlet oxygen detection: the misleading role of electron transfer.
abstract: For many biological and biomedical studies, it is essential to detect the production of (1)O2 and quantify its production yield. Among the available methods, detection of the characteristic 1270-nm phosphorescence of singlet oxygen by time-resolved near-infrared (TRNIR) emission constitutes the most direct and unambiguous approach. An alternative indirect method is electron paramagnetic resonance (EPR) in combination with a singlet oxygen probe. This is based on the detection of the TEMPO free radical formed after oxidation of TEMP (2,2,6,6-tetramethylpiperidine) by singlet oxygen. Although the TEMPO/EPR method has been widely employed, it can produce misleading data. This is demonstrated by the present study, in which the quantum yields of singlet oxygen formation obtained by TRNIR emission and by the TEMPO/EPR method are compared for a set of well-known photosensitizers. The results reveal that the TEMPO/EPR method leads to significant overestimation of singlet oxygen yield when the singlet or triplet excited state of the photosensitizer is efficiently quenched by TEMP, acting as electron donor. In such case, generation of the TEMP(+) radical cation, followed by deprotonation and reaction with molecular oxygen, gives rise to an EPR-detectable TEMPO signal that is not associated with singlet oxygen production. This knowledge is essential for an appropriate and error-free application of the TEMPO/EPR method in chemical, biological, and medical studies.
authors.affiliation: Instituto de Tecnología Química UPV-CSIC, Universitat Politècnica de València, 46022 Valencia, Spain.
authors.forename: Giacomo
authors.initials: G
authors.lastname: Nardi
authors.affiliation: Istituto per la Sintesi Organica e la Fotoreattività, Consiglio Nazionale delle Ricerche, 40129 Bologna, Italy.
authors.forename: Ilse
authors.initials: I
authors.lastname: Manet
authors.affiliation: Istituto per la Sintesi Organica e la Fotoreattività, Consiglio Nazionale delle Ricerche, 40129 Bologna, Italy.
authors.forename: Sandra
authors.initials: S
authors.lastname: Monti
authors.affiliation: Instituto de Tecnología Química UPV-CSIC, Universitat Politècnica de València, 46022 Valencia, Spain.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Miranda
authors.affiliation: Instituto de Tecnología Química UPV-CSIC, Universitat Politècnica de València, 46022 Valencia, Spain. Electronic address: lvirgini@itq.upv.es.
authors.forename: Virginie
authors.initials: V
authors.lastname: Lhiaubet-Vallet
mesh_terms: D003497:Cyclic N-Oxides / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D010084:Oxidation-Reduction; D010782:Photolysis; D017319:Photosensitizing Agents / Q000737:chemistry; D026082:Singlet Oxygen / Q000032:analysis*
chemical_list: D003497:Cyclic N-Oxides; D017319:Photosensitizing Agents; D026082:Singlet Oxygen; C003959:TEMPO
keywords: EPR; Free radicals; Photosensitizer; Singlet oxygen; TEMPO; Time-resolved near-infrared emission