title: si-RNA-mediated knockdown of PDLIM5 suppresses gastric cancer cell proliferation in vitro.
abstract: Gastric cancer is the second most prominent cause of cancer mortality in the world. This study was designed to identify the possible use of si-RNA-mediated PDLIM5 gene silencing as a therapeutic tool for gastric cancer. Expression levels of PDLIM5 were detected in several gastric cancer cell lines using Western blot and qRT-PCR. We found PDLIM5 is highly expressed in all cultured gastric cancer cell lines. Small interfering RNA (si-RNA) was then employed to knock down PDLIM5 expression in MGC80-3 gastric cancer cells. Knockdown of PDLIM5 significantly inhibited cell proliferation and colony formation. Moreover, the absence of PDLIM5 in MGC80-3 cells led to S phase cell cycle arrest and apoptosis. This study highlights the critical role of PDLIM5 in gastric cancer cell growth and suggests that si-RNA-mediated silencing of PDLIM5 might serve as a potential therapeutic approach for the treatment of gastric cancer.
authors.affiliation: Department of Gastrointestinal Surgery, Shandong Tumor Hospital, Jinan, 250117, China.
authors.forename: Yanliang
authors.initials: Y
authors.lastname: Li
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Gao
authors.forename: Yue
authors.initials: Y
authors.lastname: Xu
authors.forename: Xianjun
authors.initials: X
authors.lastname: Sun
authors.forename: Xilin
authors.initials: X
authors.lastname: Song
authors.forename: Heng
authors.initials: H
authors.lastname: Ma
authors.forename: Mingshan
authors.initials: M
authors.lastname: Yang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D060588:LIM Domain Proteins / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000627:therapeutic use*; D000067492:RNAi Therapeutics / Q000379:methods; D013270:Stomach / Q000166:cytology / Q000473:pathology; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D060588:LIM Domain Proteins; C512485:PDLIM5 protein, human; D034741:RNA, Small Interfering
keywords: PDLIM5; cell cycle; gastric cancer; growth; si-RNA