title: Molecular characterization of a proteolysis-resistant lipase from Bacillus pumilus SG2.
abstract: Proteolysis-resistant lipases can be well exploited by industrial processes which employ both lipase and protease as biocatalysts. A proteolysis resistant lipase from Bacillus pumilus SG2 was isolated, purified and characterized earlier. The lipase was resistant to native and commercial proteases. In the present work, we have characterized the lip gene which encodes the proteolysis-resistant lipase from Bacillus pumilus SG2. The parameters and structural details of lipase were analysed. The lip gene consisted of 650 bp. The experimental molecular weight of SG2 lipase was nearly double that of its theoretical molecular weight, thus suggesting the existence of the functional lipase as a covalent dimer. The proteolytic cleavage sites of the lipase would have been made inaccessible by dimerisation, thus rendering the lipase resistant to protease.
authors.affiliation: Department of Biochemistry School of Life Sciences Vels University Chennai India Department of Biochemistry, School of Life Sciences, Vels University, Chennai, India.
authors.forename: R
authors.initials: R
authors.lastname: Sangeetha
authors.affiliation: Research Department of Microbiology Asan Memorial College Chennai India Research Department of Microbiology, Asan Memorial College, Chennai, India.
authors.forename: I
authors.initials: I
authors.lastname: Arulpandi
authors.affiliation: Department of Biochemistry Bharathi Women's College Chennai India Department of Biochemistry, Bharathi Women's College, Chennai, India.
authors.forename: A
authors.initials: A
authors.lastname: Geetha
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D008049:Lipase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000378:metabolism; D010802:Phylogeny; D055503:Protein Multimerization; D059748:Proteolysis; D017385:Sequence Homology
chemical_list: D008049:Lipase; D010447:Peptide Hydrolases
keywords: Bacillus pumilus; covalent dimer; lipase; proteolytic-resistance