title: Poly ICLC increases the potency of a replication-defective human adenovirus vectored foot-and-mouth disease vaccine.
abstract: Foot-and-mouth disease virus (FMDV) causes a highly contagious disease of cloven-hoofed animals. We have previously demonstrated that a replication-defective human adenovirus 5 vector carrying the FMDV capsid coding region of serotype A24 Cruzeiro (Ad5-CI-A24-2B) protects swine and cattle against FMDV challenge by 7 days post-vaccination. However, since relatively large amounts of Ad5-CI-A24-2B are required to induce protection this strategy could be costly for livestock production. Poly ICLC is a synthetic double stranded RNA that activates multiple innate and adaptive immune pathways. In this study, we have tested for the first time, the adjuvant effect of poly ICLC in combination with Ad5-CI-A24-2B in swine. We found that the combination resulted in a reduction of the vaccine protective dose by 80-fold. Interestingly, the lowest dose of Ad5-CI-A24-2B plus 1mg of poly ICLC protected animals against challenge even in the absence of detectable FMDV-specific neutralizing antibodies at the time of challenge.
authors.affiliation: Plum Island Animal Disease Center, North Atlantic Area, Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, United States.
authors.forename: Fayna
authors.initials: F
authors.lastname: Diaz-San Segundo
authors.affiliation: Plum Island Animal Disease Center, North Atlantic Area, Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, United States; Oak Ridge Institute for Science and Education, PIADC Research Participation Program, Oak Ridge, TN 37831, United States.
authors.forename: Camila C
authors.initials: CC
authors.lastname: Dias
authors.affiliation: Plum Island Animal Disease Center, North Atlantic Area, Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, United States; Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06269, United States.
authors.forename: Mauro P
authors.initials: MP
authors.lastname: Moraes
authors.affiliation: Plum Island Animal Disease Center, North Atlantic Area, Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, United States; Oak Ridge Institute for Science and Education, PIADC Research Participation Program, Oak Ridge, TN 37831, United States.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Weiss
authors.affiliation: Plum Island Animal Disease Center, North Atlantic Area, Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, United States; Oak Ridge Institute for Science and Education, PIADC Research Participation Program, Oak Ridge, TN 37831, United States.
authors.forename: Eva
authors.initials: E
authors.lastname: Perez-Martin
authors.affiliation: Oncovir, Inc., Washington, DC 20008, United States.
authors.forename: Andres M
authors.initials: AM
authors.lastname: Salazar
authors.affiliation: Plum Island Animal Disease Center, North Atlantic Area, Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, United States. Electronic address: marvin.grubman@ars.usda.gov.
authors.forename: Marvin J
authors.initials: MJ
authors.lastname: Grubman
authors.affiliation: Plum Island Animal Disease Center, North Atlantic Area, Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, United States. Electronic address: teresa.delossantos@ars.usda.gov.
authors.forename: Teresa
authors.initials: T
authors.lastname: de Los Santos
mesh_terms: D000260:Adenoviruses, Human; D000818:Animals; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral / Q000097:blood; D002266:Carboxymethylcellulose Sodium / Q000031:analogs & derivatives* / Q000494:pharmacology; D005536:Foot-and-Mouth Disease / Q000517:prevention & control*; D005822:Genetic Vectors; D057809:HEK293 Cells; D006801:Humans; D011070:Poly I-C / Q000494:pharmacology*; D011107:Polylysine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control; D014765:Viral Vaccines / Q000276:immunology*; D014779:Virus Replication
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D014765:Viral Vaccines; D011107:Polylysine; C019531:poly ICLC; D002266:Carboxymethylcellulose Sodium; D011070:Poly I-C
keywords: Adjuvants; Cell-mediated immunity; Foot-and-mouth disease; Foot-and-mouth disease virus; Neutralizing antibodies; Poly ICLC; Replication-defective adenovirus; Vaccines