title: Regulation of mitotic spindle disassembly by an environmental stress-sensing pathway in budding yeast.
abstract: Timely spindle disassembly is essential for coordination of mitotic exit with cytokinesis. In the budding yeast Saccharomyces cerevisiae, the microtubule-associated protein She1 functions in one of at least three parallel pathways that promote spindle disassembly. She1 phosphorylation by the Aurora kinase Ipl1 facilitates a role for She1 in late anaphase, when She1 contributes to microtubule depolymerization and shrinkage of spindle halves. By examining the genetic interactions of known spindle disassembly genes, we identified three genes in the environmental stress-sensing HOG (high-osmolarity glycerol response) pathway, SHO1, PBS2, and HOG1, and found they are necessary for proper localization of She1 to the anaphase spindle and for proper spindle disassembly. HOG pathway mutants exhibited spindle disassembly defects, as well as mislocalization of anillin-related proteins Boi1 and Boi2 from the bud neck. Moreover, Boi2, but not Boi1, plays a role in spindle disassembly that places Boi2 in a pathway with Sho1, Pbs2, and Hog1. Together, our data identify a process by which cells monitor events at the spindle and bud neck and describe a novel role for the HOG pathway in mitotic signaling.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, California 94720.
authors.forename: Adrianne
authors.initials: A
authors.lastname: Pigula
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, California 94720.
authors.forename: David G
authors.initials: DG
authors.lastname: Drubin
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 gbarnes@berkeley.edu.
authors.forename: Georjana
authors.initials: G
authors.lastname: Barnes
mesh_terms: D048749:Cytokinesis; D004777:Environment; D008938:Mitosis; D009154:Mutation / Q000235:genetics; D058105:Polymerization; D011485:Protein Binding; D021381:Protein Transport; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D004718:Saccharomycetales / Q000166:cytology* / Q000378:metabolism*; D015398:Signal Transduction; D008941:Spindle Apparatus / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D029701:Saccharomyces cerevisiae Proteins
keywords: Hog1; NoCut pathway; She1; high-osmolarity glycerol response (HOG) pathway; spindle disassembly