title: New vitamin E isomers (gamma-tocomonoenol and alpha-tocomonoenol) in seeds, roasted seeds and roasted seed oil from the Slovenian pumpkin variety 'Slovenska golica'.
abstract: The Štajerska region in north-eastern Slovenia and the Styria region in southern Austria have a long tradition of growing pumpkins (Cucurbita pepo L.) as an oil crop. GC-MS determination of the free and esterified minor compounds in oil of roasted pumpkin seeds from the Slovenian C. pepo L. variety 'Slovenska golica' revealed the presence of two previously unreported compounds: alpha-tocomonoenol and gamma-tocomonoenol. Using the GC-MS data, reference samples (Crude Palm Oil) and tocopherol and tocotrienol standards it was possible to assign and quantify alpha-tocomonoenol (17.6±0.6μg/g) and gamma-tocomonoenol (118.7±1.0μg/g) compounds in roasted 'S. golica' seed oil using HPLC. The concentrations of alpha-tocopherol and gamma-tocopherol were 77.9±1.9μg/g and 586.0±4.6μg/g, respectively. Surprisingly the gamma-tocotrienol concentration found was only 6.9±0.2μg/g. Analysis of the seeds from which the oil was pressed showed the initial gamma-tocotrienol amount was even lower (1.6±0.1 and 2.2±0.1μg/g in the ground and roasted seeds, respectively) than in the roasted seed oil.
authors.affiliation: Laboratory for Olive Oil Testing, Science and Research Centre of Koper, University of Primorska, Zelena ulica 8, SI-6310 Izola, Slovenia.
authors.forename: Bojan
authors.initials: B
authors.lastname: Butinar
authors.affiliation: Laboratory for Olive Oil Testing, Science and Research Centre of Koper, University of Primorska, Zelena ulica 8, SI-6310 Izola, Slovenia; LABS, LLC - Institute for Ecology, Olive Oil and Control, Zelena ulica 8, SI-6310 Izola, Slovenia.
authors.forename: Milena
authors.initials: M
authors.lastname: Bučar-Miklavčič
authors.affiliation: Stazione Sperimentale per le Industrie degli Oli e dei Grassi, Via Giuseppe Colombo 79, 20133 Milano, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Mariani
authors.affiliation: Food Science and Technology Department, Chair of Biotechnology, Microbiology and Food Safety, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000, Ljubljana, Slovenia. Electronic address: peter.raspor@bf.uni-lj.si.
authors.forename: Peter
authors.initials: P
authors.lastname: Raspor
keywords: Alpha-tocomonoenol; Cucurbita pepo L.; GC-MS; Gamma-tocomonoenol; HPLC; Pumpkin seed oil; Vitamin E