title: Generation of reactive oxygen species by interaction between antioxidants used as food additive and metal ions.
abstract: Food additives, such as preservatives, sweeteners, coloring agents, and flavoring agents, are widely used in food manufacturing. However, their combined effects on the human body are not known. The purpose of this study was to examine whether combinations of antioxidants and metal ions generate reactive oxygen species (ROS) under in vitro conditions using electron spin resonance (ESR). Among the metal ions examined, only iron and copper generated ROS in the presence of antioxidants. Moreover, certain phenolic antioxidants having pro-oxidant activity induced DNA oxidation and degradation via the generation of high levels of ROS in the presence of copper ion, resulting in complete degradation of DNA in vitro.
authors.affiliation: Department of Analytical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: Momoko
authors.initials: M
authors.lastname: Oda
authors.forename: Yuri
authors.initials: Y
authors.lastname: Tsukuda
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nagamori
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakazawa
authors.forename: Rie
authors.initials: R
authors.lastname: Ito
authors.forename: Koichi
authors.initials: K
authors.lastname: Saito
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D005503:Food Additives / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D007477:Ions; D007501:Iron / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D010084:Oxidation-Reduction; D010636:Phenols / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D005503:Food Additives; D007477:Ions; D010636:Phenols; D017382:Reactive Oxygen Species; D003300:Copper; D007501:Iron