title: Cytocompatibility, gene-expression profiling, apoptotic, mechanical and (29)Si, (31)P solid-state nuclear magnetic resonance studies following treatment with a bioglass-chitosan composite.
abstract: The performance therapy of chitosan (CH)-doped bioactive glass (BG) has been evaluated in vitro and in vivo. In vitro, the effect of CH-BG was assessed on human Saos-2 osteoblast cells. In vivo, Wistar rats were ovariectomized (OVX) and CH, BG and CH-BG were implanted in bone tissue. After 3 days of CH-BG contact, cell viability of Saos-2 osteoblast increased by 16.4% as compared to the control group. The runt-related transcription factor 2 (RUNX2/Cbfa1) and osteocalcin (OC) gene expressions were significantly increased with 600 and 300%, respectively, in contact of CH-BG as compared with CH. In vivo, the apoptotic index in the OVX-CH-BG group was decreased by 80%. A mechanical hardness test showed a significant bone strength improvement after CH-BG implantation (40%). The CH-BG composite may therefore prove clinically useful as a bioactive bone substitute.
authors.affiliation: University of Rennes 1, UMR CNRS 6226, Campus de Beaulieu, 263 av. du Général Leclerc, 35042, Rennes, France, jbahisamira@yahoo.fr.
authors.forename: Jebahi
authors.initials: J
authors.lastname: Samira
authors.forename: Oudadesse
authors.initials: O
authors.lastname: Hassane
authors.forename: Saoudi
authors.initials: S
authors.lastname: Mongi
authors.forename: Kallabi
authors.initials: K
authors.lastname: Fakhri
authors.forename: Pascal
authors.initials: P
authors.lastname: Pellen
authors.forename: Rebai
authors.initials: R
authors.lastname: Treq
authors.forename: Elfeki
authors.initials: E
authors.lastname: Abdelfatteh
authors.forename: Keskes
authors.initials: K
authors.lastname: Hassib
mesh_terms: D000818:Animals; D017209:Apoptosis; D018786:Bone Substitutes / Q000008:administration & dosage / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002516:Ceramics / Q000378:metabolism*; D048271:Chitosan / Q000008:administration & dosage / Q000378:metabolism*; D050659:Core Binding Factor Alpha 1 Subunit / Q000096:biosynthesis; D020869:Gene Expression Profiling; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015675:Osteocalcin / Q000096:biosynthesis; D017208:Rats, Wistar
chemical_list: C022107:Bioglass; D018786:Bone Substitutes; D050659:Core Binding Factor Alpha 1 Subunit; D015675:Osteocalcin; D048271:Chitosan