title: Preparation of thermosensitive polymer magnetic particles and their application in protein separations.
abstract: This paper presents a kind of thermoresponsive polymeric magnetic particles for protein separations. The magnetofluids were directly encapsulated in hollow particles constructed by self-assembly of rod-coil poly(ethylene glycol)-poly(N-isopropylacrylamide)/α-cyclodextrin (PEG-PNIPAM/α-CD) complexes. The resulting particles showed reversible protein absorption/desorption capacity because the reversible thermo-sensitivity of PNIPAM. Above the lower critical solution temperature (LCST) of PNIPAM, these particles showed high absorptive capacities and adsorption was done at lower temperature. The protein-laden particles are readily removed from the feed solution in a magnetic field.
authors.affiliation: State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Sichuan University, Chengdu 610065, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Sichuan University, Chengdu 610065, China.
authors.forename: Hong-Su
authors.initials: HS
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Sichuan University, Chengdu 610065, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ha
authors.affiliation: State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Sichuan University, Chengdu 610065, China.
authors.forename: Luo-Hao
authors.initials: LH
authors.lastname: Li
authors.affiliation: State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Sichuan University, Chengdu 610065, China.
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Gong
authors.affiliation: Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China. Electronic address: libj@cib.ac.cn.
authors.forename: Bang-Jing
authors.initials: BJ
authors.lastname: Li
authors.affiliation: State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Sichuan University, Chengdu 610065, China. Electronic address: zslbj@163.com.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D005290:Ferric Compounds / Q000737:chemistry; D060328:Magnetic Phenomena; D009113:Muramidase / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011506:Proteins / Q000302:isolation & purification*; D013696:Temperature
chemical_list: D005290:Ferric Compounds; D011108:Polymers; D011506:Proteins; C000499:ferric oxide; D011092:Polyethylene Glycols; D009113:Muramidase
keywords: Host–guest recognition; Lysozyme; Magnetic particles; Self-assembly