title: Potential of Monoraphidium minutum for carbon sequestration and lipid production in response to varying growth mode.
abstract: Mixotrophic growth at flask level and, autotrophic-mixotrophic and autotrophic growth in photobioreactor by utilizing CO2/air/flue gas were checked for the isolated strain of Monoraphidium minutum from polluted habitat. Our study confirmed that it is a saturated fatty acid rich (30.92-68.94%) microalga with lower degree of unsaturation oil quality (42.06-103.99) making it potential biodiesel producing candidate. It showed encouraging biomass productivity (80.3-303.8mgl(-1)day(-1)) with higher total lipid (22.80-46.54%) under optimum glucose, fructose, microalgal biodiesel waste residue and sodium acetate fed mixotrophic conditions. The pH control by intermittent CO2, continuous illumination with 30% flue gas, and utilization of biodiesel glycerin were effective schemes to ameliorate either biomass productivity or % lipids or both of these parameters at photobioreactor scale (7.5L working volume). The modulation of environmental variables (pH control, CO2 and organic substrates concentration) could augment % saturated fatty acids, such as C16:0.
authors.affiliation: Salt and Marine Chemicals Discipline, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: Shailesh Kumar
authors.initials: SK
authors.lastname: Patidar
authors.affiliation: Salt and Marine Chemicals Discipline, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: Madhusree
authors.initials: M
authors.lastname: Mitra
authors.affiliation: Salt and Marine Chemicals Discipline, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: Basil
authors.initials: B
authors.lastname: George
authors.affiliation: Salt and Marine Chemicals Discipline, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors.forename: R
authors.initials: R
authors.lastname: Soundarya
authors.affiliation: Salt and Marine Chemicals Discipline, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India. Electronic address: smishra@csmcri.org.
authors.forename: Sandhya
authors.initials: S
authors.lastname: Mishra
mesh_terms: D052818:Autotrophic Processes; D056804:Biofuels / Q000382:microbiology; D018533:Biomass; D002244:Carbon / Q000032:analysis; D057965:Carbon Sequestration; D005227:Fatty Acids / Q000032:analysis; D005612:Freeze Drying; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000254:growth & development* / Q000378:metabolism* / Q000648:ultrastructure; D009584:Nitrogen / Q000032:analysis; D009930:Organic Chemicals / Q000032:analysis; D058433:Photobioreactors / Q000382:microbiology; D012995:Solubility
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D008055:Lipids; D009930:Organic Chemicals; D002244:Carbon; D009584:Nitrogen
keywords: Biodiesel; Flue gas; Intermittent CO(2) supply; Mixotrophic; Monoraphidium minutum