title: The Low Prevalence of Y Chromosomal Microdeletions is Observed in the Oligozoospermic Men in the Area of Mato Grosso State and Amazonian Region of Brazilian Patients.
abstract: OBJECTIVE
  To determine the prevalence of chromosomal abnormalities and microdeletions on Y chromosome in infertile patients with oligozoospermia or azoospermia in Mato Grosso state, Brazil.
  
  
  METHODS
  This cross-sectional study enrolled 94 men from infertile couples. Karyotype analysis was performed by lymphocyte culture technique. DNA from each sample was extracted using non-enzymatic method. Microdeletions were investigated by polymerase chain reaction (PCR).
  
  
  RESULTS
  With the use of cytogenetic analysis, five patients (5.3%) had abnormal karyotype, one azoospermic patient (1.1%) had karyotype 46,XY,t(7;1) (qter-p35), one (1.1%) with mild oligozoospermia had karyotype 46,XY,delY(q), and two other azoospermic patients had karyotype 47,XXY, consistent with Klinefelter syndrome (KS). One of them (1.1%) with severe oligozoospermia had karyotype 46,XY,8p+. Microdeletion on Y chromosome was found in the azoospermia factor c (AZFc) region in only one azoospermic patient (1.1%).
  
  
  CONCLUSIONS
  The prevalence of genetic abnormalities in oligo/azoospermic Brazilian men from infertile couple was 5.3%, and microdeletion on Y chromosome was not a common finding in this population (1.1%).
authors.affiliation: Department of Basic Sciences, Medical School, UFMT, Cuiabá, MT, Brazil.
authors.forename: Gleice Cristina
authors.initials: GC
authors.lastname: Dos Santos Godoy
authors.affiliation: Department of Basic Sciences, Medical School, UFMT, Cuiabá, MT, Brazil.
authors.forename: Bianca Borsatto
authors.initials: BB
authors.lastname: Galera
authors.affiliation: Faculty of Biology, Federal University of Mato Grosso, UFMT, Cuiabá, MT, Brazil.
authors.forename: Claudinéia
authors.initials: C
authors.lastname: Araujo
authors.affiliation: Tropical Institute of Reproductive Medicine and Menopause, Cuiabá, MT, Brazil.
authors.forename: Jacklyne Silva
authors.initials: JS
authors.lastname: Barbosa
authors.affiliation: Faculty of Biology, Federal University of Mato Grosso, UFMT, Cuiabá, MT, Brazil.
authors.forename: Max Fernando
authors.initials: MF
authors.lastname: de Pinho
authors.affiliation: Department of Basic Sciences, Medical School, UFMT, Cuiabá, MT, Brazil.
authors.forename: Marcial Francis
authors.initials: MF
authors.lastname: Galera
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine, UFMT, Cuiabá, MT, Brazil. ; Tropical Institute of Reproductive Medicine and Menopause, Cuiabá, MT, Brazil.
authors.forename: Sebastião Freitas
authors.initials: SF
authors.lastname: de Medeiros
keywords: Y chromosome; cytogenetic; infertile male; microdeletion