title: Effect of trichostatin a on SGC-7901 gastric cancer cells.
abstract: OBJECTIVE
  To explore Trichostatin A (TSA) effect on SGC-7901 gastric cancer cells.
  
  
  METHODS
  MTT, fluorescence microscopy, and flow cytometry were used to assess TSA effect on cell growth and apoptosis in SGC-7901. Immunocytochemistry was used to evaluate the expression of acetylated histone H4 in SGC-7901 cells.Gene expression profile was determined by microarray assays. Glycoprotein hormones alpha subunit (CGA) gene and protein expressions in SGC-7901 cells were evaluated by Real-time PCR and Western blot, respectively. In addition, CGA protein levels in gastric adenocarcinoma and normal adjacent tissues were assessed by immunohistochemistry.
  
  
  RESULTS
  TSA inhibited SGC-7901 cell growth. In addition, cell proliferation was significantly decreased (P = 0.02) in TSA treatment groups (0.93 ± 0.07) compared with controls (1.15 ± 0.07). Apoptosis related morphological changes, including nuclear chromatin condensation and fluorescence strength, were observed by fluorescence microscopy. These findings corroborated the increased expression of acetylated histone H4 observed in TSA treated cells compared to controls, as determined by immunocytochemistry. Interestingly, treatment of SGC-7901 cells with TSA (75 ng/ml) resulted in CGA gene down-regulation (P = 0.0381). Accordingly, CGA protein levels were decreased in TSA treated SGC-7901 cells. Finally, immunohistochemistry analysis showed that CGA expression was significantly higher in gastric adenocarcinoma tissues than normal adjacent tissues (P = 0.001).
  
  
  CONCLUSIONS
  TSA induces cell apoptosis and increases the levels of acetylated histone H4 in SGC-7901 cells. In addition, TSA treatment decreases the expression in gastric cancer cells of the CGA gene, which is upregulated in gastric adenocarcinoma tissues.
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University Harbin 150081, Heilongjiang Province, China.
authors.forename: Yun-Long
authors.initials: YL
authors.lastname: Li
authors.affiliation: Department of Laboratory, The Third Affiliated Hospital of Harbin Medical University Harbin 150081, Heilongjiang Province, China.
authors.forename: Tong-Shu
authors.initials: TS
authors.lastname: Yang
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University Harbin 150081, Heilongjiang Province, China.
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Ruan
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University Harbin 150081, Heilongjiang Province, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Cui
authors.affiliation: Key Laboratory of Medical Genetics (Harbin Medical University), Heilongjiang Higher Education Institutions Harbin 150081, Heilongjiang Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University Harbin 150081, Heilongjiang Province, China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Zou
keywords: Gastric cancer; SGC-7901; apoptosis; glycoprotein hormones α subunit; histone H4; trichostatin A