title: A thermosensitive defect in the ATP binding pocket of FtsA can be suppressed by allosteric changes in the dimer interface.
abstract: In Escherichia coli, initial assembly of the Z ring for cell division requires FtsZ plus the essential Z ring-associated proteins FtsA and ZipA. Thermosensitive mutations in ftsA, such as ftsA27, map in or near its ATP binding pocket and result in cell division arrest at non-permissive temperatures. We found that purified wild-type FtsA bound and hydrolysed ATP, whereas FtsA27 was defective in both activities. FtsA27 was also less able to localize to the Z ring in vivo. To investigate the role of ATP transactions in FtsA function in vivo, we isolated intragenic suppressors of ftsA27. Suppressor lesions in the ATP site restored the ability of FtsA27 to compete with ZipA at the Z ring, and enhanced ATP binding and hydrolysis in vitro. Notably, suppressors outside of the ATP binding site, including some mapping to the FtsA-FtsA subunit interface, also enhanced ATP transactions and exhibited gain of function phenotypes in vivo. These results suggest that allosteric effects, including changes in oligomeric state, may influence the ability of FtsA to bind and/or hydrolyse ATP.
authors.affiliation: Department of Microbiology & Molecular Genetics, University of Texas Health Science Center at Houston, 6431 Fannin St., Houston, TX, 77030, USA.
authors.forename: Jennifer R
authors.initials: JR
authors.lastname: Herricks
authors.forename: Diep
authors.initials: D
authors.lastname: Nguyen
authors.forename: William
authors.initials: W
authors.lastname: Margolin
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006868:Hydrolysis; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020125:Mutation, Missense; D011485:Protein Binding; D011487:Protein Conformation; D055503:Protein Multimerization / Q000528:radiation effects*; D013489:Suppression, Genetic; D013696:Temperature
chemical_list: D029968:Escherichia coli Proteins; C489310:FtsA protein, E coli; D050505:Mutant Proteins; D000255:Adenosine Triphosphate