title: L-FABP and I-FABP expression in newborn rats changes inversely in the model of necrotizing enterocolitis.
abstract: OBJECTIVE
  To determine the expression of hepatic L-FABP and intestinal I-FABP in an experimental model of necrotizing enterocolitis (NEC) in neonatal rats.
  
  
  METHODS
  Newborn Sprague-Dawley rats were divided into four groups: Control (C1) - exclusive breastfeeding at the first and sixth procedures (C6), NEC1 - fed formula milk and submitted to hypoxia and hypothermia at the first and sixth procedures (NEC6). The newborn pups were fed twice a day for three days, for a total of six procedures. Samples were collected for morphometric evaluation (body weight, liver weight, liver weight/body weight ratio, intestinal weight and intestinal/body weight ratio) and for immunohistochemical and Western blotting analysis. The values obtained were analyzed statistically, with the level of significance set at p<0.05.
  
  
  RESULTS
  Morphometric measurements showed reduction of body and liver weights in the NEC group (p<0.05). Both immunohistochemistry and western blotting revealed that L-FABP expression in the liver was decreased and I-FABP expression in the ileum was increased in the NEC group (p<0.05).
  
  
  CONCLUSIONS
  L-FABP and I-FABP expression changed inversely in the rat NEC model. These findings can contribute to a better diagnosis of NEC in human newborns.
authors.affiliation: Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Luís Felipe Tsuyoshi
authors.initials: LF
authors.lastname: Mitidiero
authors.affiliation: Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Ana Leda Bertoncini
authors.initials: AL
authors.lastname: Simões
authors.affiliation: Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Frances Lilian Lanhellas
authors.initials: FL
authors.lastname: Gonçalves
authors.affiliation: Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Rebeca Rodrigues Lopes Roslindo
authors.initials: RR
authors.lastname: Figueira
authors.affiliation: Department of Surgery and Anatomy, Ribeirão Preto Medical School, Sao Paulo University, Ribeirão Preto, SP, Brazil.
authors.forename: Orlando
authors.initials: O
authors.lastname: Castro e Silva
authors.affiliation: Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Lourenço
authors.initials: L
authors.lastname: Sbragia
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D001835:Body Weight; D004195:Disease Models, Animal; D020345:Enterocolitis, Necrotizing / Q000378:metabolism* / Q000473:pathology; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism*; D005260:Female; D007082:Ileum / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009929:Organ Size; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D012016:Reference Values
chemical_list: D050556:Fatty Acid-Binding Proteins