title: Comparative evaluation of Salmonella Enteritidis ghost vaccines with a commercial vaccine for protection against internal egg contamination with Salmonella.
abstract: The study was conducted for the comparative evaluation of the vaccine potential of Salmonella Enteritidis (S. Enteritidis, SE) ghost, SE ghost carrying Escherichia coli heat labile enterotoxin B subunit (LTB) protein, and a commercial vaccine. Group A chickens were used as a non-vaccinated control, group B chickens were immunized with the ghost carrying LTB protein, group C chickens were immunized with the ghost and, group D chickens were immunized with a commercial vaccine. Group D chickens showed the swelling at the injection site, while no adverse reactions were observed at injection sites of the group B and C chickens. Chickens from the immunized groups B, C, and D demonstrated significant increases in plasma IgG, intestinal secretory IgA levels, and antigen-specific lymphocyte proliferative responses. After challenge with a virulent SE strain via intravenous route, groups B, C, and D showed significantly higher egg production and lower internal egg contamination and lower recovery of the challenge strain from internal organs compared to non-immunized-challenged control group A. In conclusion, these data indicate that immunization of chickens with the ghost and ghost carrying LTB is safe, without causing any adverse reaction, and is effective as the commercial vaccine in terms of reduction in internal egg contamination and internal organ colonization of Salmonella.
authors.affiliation: College of Veterinary Medicine, Chonbuk National University, Veterinary Public Health, 664-14 Duckjin-dong, Jeonju 561-756, Republic of Korea.
authors.forename: Chetan V
authors.initials: CV
authors.lastname: Jawale
authors.affiliation: College of Veterinary Medicine, Chonbuk National University, Veterinary Public Health, 664-14 Duckjin-dong, Jeonju 561-756, Republic of Korea. Electronic address: johnhlee@jbnu.ac.kr.
authors.forename: John Hwa
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000276:immunology; D049109:Cell Proliferation; D002645:Chickens; D004531:Eggs / Q000382:microbiology*; D004768:Enterotoxins / Q000276:immunology; D029968:Escherichia coli Proteins / Q000276:immunology; D005260:Female; D005506:Food Contamination / Q000517:prevention & control; D056724:Immunity, Humoral; D007071:Immunoglobulin A, Secretory / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D011201:Poultry Diseases / Q000517:prevention & control*; D012481:Salmonella Infections, Animal / Q000517:prevention & control*; D022562:Salmonella Vaccines / Q000276:immunology*; D012477:Salmonella enteritidis / Q000276:immunology
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D022562:Salmonella Vaccines; C027538:heat-labile enterotoxin, E coli
keywords: Egg contamination; LTB adjuvant; Protection; S. Enteritidis ghost