title: Genetic diversity of livestock-associated MRSA isolates obtained from piglets from farrowing until slaughter age on four farrow-to-finish farms.
abstract: During a previous longitudinal study, performed on four farrow-to-finish farms (A to D), samples were taken from twelve sows, their offspring, and the environment on various occasions over six months to study the MRSA presence. During the present study, a selection of the obtained MRSA isolates were typed by multiple-locus variable-number tandem-repeat analysis (MLVA), Pulsed Field Gel Electrophoresis (PFGE), spa typing, and SCCmec typing to study the genetic diversity of LA-MRSA isolates and to determine possible MRSA sources for pig(let)s. PFGE, spa typing, and SCCmec typing revealed the presence of one or few dominant genotype(s) per farm. In contrast, 212 MLVA types were detected on the four farms, forming one cluster on farm A, three on farm B, four on farm C and two on farm D. The genotype, found on farm A was unique for this farm. Farms B, C and D shared one cluster. In general, MLVA types from these clusters were isolated from piglets, sows, and the environment on various sampling events. Piglets carried MLVA types both related and unrelated to their mother sows' MLVA types at farrowing and onwards. In conclusion, molecular typing revealed that within a farm one or a few dominant strain(s) are widespread. Potential MRSA sources for piglets were mother sows, the environment and other piglets.
authors.forename: Marijke
authors.initials: M
authors.lastname: Verhegghe
authors.forename: Florence
authors.initials: F
authors.lastname: Crombé
authors.forename: Larissa J
authors.initials: LJ
authors.lastname: Pletinckx
authors.forename: Freddy
authors.initials: F
authors.lastname: Haesebrouck
authors.forename: Patrick
authors.initials: P
authors.lastname: Butaye
authors.forename: Lieve
authors.initials: L
authors.lastname: Herman
authors.forename: Marc
authors.initials: M
authors.lastname: Heyndrickx
authors.forename: Geertrui
authors.initials: G
authors.lastname: Rasschaert
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D014644:Genetic Variation; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics* / Q000378:metabolism; D018598:Minisatellite Repeats; D058885:Multilocus Sequence Typing / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins