title: Controlled expression of pectic enzymes in Arabidopsis thaliana enhances biomass conversion without adverse effects on growth.
abstract: Lignocellulosic biomass from agriculture wastes is a potential source of biofuel, but its use is currently limited by the recalcitrance of the plant cell wall to enzymatic digestion. Modification of the wall structural components can be a viable strategy to overcome this bottleneck. We have previously shown that the expression of a fungal polygalacturonase (pga2 from Aspergillus niger) in Arabidopsis and tobacco plants reduces the levels of de-esterified homogalacturonan in the cell wall and significantly increases saccharification efficiency. However, plants expressing pga2 show stunted growth and reduced biomass production, likely as a consequence of an extensive loss of pectin integrity during the whole plant life cycle. We report here that the expression in Arabidopsis of another pectic enzyme, the pectate lyase 1 (PL1) of Pectobacterium carotovorum, under the control of a chemically inducible promoter, results, after induction of the transgene, in a saccharification efficiency similar to that of plants expressing pga2. However, lines with high levels of transgene induction show reduced growth even in the absence of the inducer. To overcome the problem of plant fitness, we have generated Arabidopsis plants that express pga2 under the control of the promoter of SAG12, a gene expressed only during senescence. These plants expressed pga2 only at late stages of development, and their growth was comparable to that of WT plants. Notably, leaves and stems of transgenic plants were more easily digested by cellulase, compared to WT plants, only during senescence. Expression of cell wall-degrading enzymes at the end of the plant life cycle may be therefore a useful strategy to engineer crops unimpaired in biomass yield but improved for bioconversion.
authors.affiliation: Dipartimento di Biologia e Biotecnologie "Charles Darwin", Sapienza Università di Roma, Piazzale Aldo Moro, 5, 00185 Roma, Italy.
authors.forename: Susanna
authors.initials: S
authors.lastname: Tomassetti
authors.affiliation: Dipartimento di Biologia e Biotecnologie "Charles Darwin", Sapienza Università di Roma, Piazzale Aldo Moro, 5, 00185 Roma, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Pontiggia
authors.affiliation: Dipartimento di Biologia e Biotecnologie "Charles Darwin", Sapienza Università di Roma, Piazzale Aldo Moro, 5, 00185 Roma, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Verrascina
authors.affiliation: Dipartimento di Biologia e Biotecnologie "Charles Darwin", Sapienza Università di Roma, Piazzale Aldo Moro, 5, 00185 Roma, Italy.
authors.forename: Ida Barbara
authors.initials: IB
authors.lastname: Reca
authors.affiliation: Dipartimento di Biologia e Biotecnologie "Charles Darwin", Sapienza Università di Roma, Piazzale Aldo Moro, 5, 00185 Roma, Italy.
authors.forename: Fedra
authors.initials: F
authors.lastname: Francocci
authors.affiliation: Dipartimento di Biologia e Biotecnologie "Charles Darwin", Sapienza Università di Roma, Piazzale Aldo Moro, 5, 00185 Roma, Italy.
authors.forename: Gianni
authors.initials: G
authors.lastname: Salvi
authors.affiliation: Dipartimento di Biologia e Biotecnologie "Charles Darwin", Sapienza Università di Roma, Piazzale Aldo Moro, 5, 00185 Roma, Italy.
authors.forename: Felice
authors.initials: F
authors.lastname: Cervone
authors.affiliation: Dipartimento di Biologia e Biotecnologie "Charles Darwin", Sapienza Università di Roma, Piazzale Aldo Moro, 5, 00185 Roma, Italy. Electronic address: simone.ferrari@uniroma1.it.
authors.forename: Simone
authors.initials: S
authors.lastname: Ferrari
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D018533:Biomass; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism; D044043:Pectobacterium / Q000201:enzymology / Q000235:genetics; D011133:Polysaccharide-Lyases / Q000235:genetics* / Q000378:metabolism
chemical_list: D011133:Polysaccharide-Lyases; C023911:pectate lyase; D005947:Glucose
keywords: Arabidopsis thaliana; Biofuel; Cell wall; Pectin; Saccharification