title: Chemical constituents of Marrubium vulgare as potential inhibitors of nitric oxide and respiratory burst.
abstract: Phytochemical investigation of the whole plant of Marrubium vulgare L., led to the isolation of three new secondary metabolites, 11-oxomarrubiin (1), vulgarcoside A (2) and 3-hydroxyapigenin-4'-O-(6"-O-p-coumaroyl)-beta-D-glucopyranoside (3), along with four known constituents 4-7 from the polar fractions of the methanolic extract. The structures of all compounds were deduced on the basis of NMR data and HRESI-MS measurements. The new constituents 1-3 exhibited moderate to low level of inhibition on nitric oxide (NO.) production. The compound 2 also showed a moderate inhibition on pro-inflammatory cytokine TNF-alpha. The new constituents 1-3 showed no inhibitory effect on Reactive Oxygen Species (ROS) production.
authors.forename: Farzana
authors.initials: F
authors.lastname: Shaheen
authors.forename: Shagufta
authors.initials: S
authors.lastname: Rasoola
authors.forename: Zafar Ali
authors.initials: ZA
authors.lastname: Shah
authors.forename: Samreen
authors.initials: S
authors.lastname: Soomro
authors.forename: Almas
authors.initials: A
authors.lastname: Jabeen
authors.forename: M Ahmed
authors.initials: MA
authors.lastname: Mesaik
authors.forename: M Iqbal
authors.initials: MI
authors.lastname: Choudhary
mesh_terms: D000818:Animals; D002460:Cell Line; D006801:Humans; D006844:Hydrocarbons, Cyclic / Q000737:chemistry / Q000494:pharmacology*; D049449:Luminescence; D008264:Macrophages / Q000187:drug effects; D027522:Marrubium / Q000737:chemistry*; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000037:antagonists & inhibitors*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011083:Polycyclic Compounds / Q000737:chemistry / Q000494:pharmacology*; D016897:Respiratory Burst / Q000187:drug effects*
chemical_list: D006844:Hydrocarbons, Cyclic; D010936:Plant Extracts; D011083:Polycyclic Compounds; D009569:Nitric Oxide