title: A new, quick, highly sensitive ultramicro-analysis method for the identification of fructose removed from fructofuranosyl-containing gluco-oligosaccharides by ESI-CID-MS/MS.
abstract: An efficient, highly sensitive, and ultramicroscale analytical method for the identification of fructose removed from fructofuranosyl-containing gluco-oligosaccharides, including malto-oligosyl fructofuranosides and oligomeric (1→2)-α-D-glucopyranosyl-(1→2)-β-D-fructofuranosides by ESI-CID-MS/MS has been developed with proven applications far superior to the existing method using NMR. With the established principle of diagnostic fragmentation by ESI-CID-MS/MS, the terminal saccharide (either glucose or fructose) can be readily and unambiguously determined at high sensitivity without a tedious derivatization process. Detection of the A-type fragmentation (0,4)A-h type ion, and (0,2)A type ion are useful as a diagnostic fragmentation tool to identify whether fructose terminal is removed from oligosaccharides. It will facilitate the efficient production of suitable oligosaccharide microarrays crucial for studies on carbohydrate-protein interaction in seeking functional carbohydrates.
authors.affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Zhang
authors.affiliation: Jiangsu Rayguang Biotech Co, Ltd, Wuxi 214125, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Ave., Wuxi 214122, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address: xbzhan@yahoo.com.
authors.forename: Xiao-Bei
authors.initials: XB
authors.lastname: Zhan
authors.affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address: CCL92122@yahoo.com.
authors.forename: Chi-Chung
authors.initials: CC
authors.lastname: Lin
mesh_terms: D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000737:chemistry; D005632:Fructose / Q000032:analysis* / Q000737:chemistry; D006031:Glycosylation; D006868:Hydrolysis; D046228:Microarray Analysis / Q000379:methods*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D058105:Polymerization; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013395:Sucrose / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: D004187:Disaccharides; D009844:Oligosaccharides; D005632:Fructose; D013395:Sucrose; C008237:kojibiose
keywords: Characteristic ion fragmentation; ESI-CID-MS/MS; Fructofuranosyl-containing gluco-oligosaccharides; Gluco-oligosaccharides