title: RNA fluorescence in situ hybridization in cultured mammalian cells.
abstract: It is now clear that long noncoding RNAs (lncRNAs) regulate a number of aspects of nuclear organization and gene expression. An important tool for the study of the distribution and function of lncRNAs is RNA fluorescence in situ hybridization (RNA-FISH). The protocols presented in this chapter describe this method in detail and also mention a number of critical points that must be considered when performing this technique.
authors.affiliation: Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, 601 S. Goodwin Avenue, Urbana, IL, 61801, USA.
authors.forename: Vidisha
authors.initials: V
authors.lastname: Tripathi
authors.forename: Jingyi
authors.initials: J
authors.lastname: Fei
authors.forename: Taekjip
authors.initials: T
authors.lastname: Ha
authors.forename: Kannanganattu V
authors.initials: KV
authors.lastname: Prasanth
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015342:DNA Probes; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008322:Mammals; D062085:RNA, Long Noncoding / Q000032:analysis*
chemical_list: D015342:DNA Probes; D062085:RNA, Long Noncoding