title: The RNA-binding protein Rbm24 is transiently expressed in myoblasts and is required for myogenic differentiation during vertebrate development.
abstract: RNA-binding proteins (RBP) contribute to gene regulation through post-transcriptional events. Despite the important roles demonstrated for several RBP in regulating skeletal myogenesis in vitro, very few RBP coding genes have been characterized during skeletal myogenesis in vertebrate embryo. In the present study we report that Rbm24, which encodes the RNA-binding motif protein 24, is required for skeletal muscle differentiation in vivo. We show that Rbm24 transcripts are expressed at all sites of skeletal muscle formation during embryogenesis of different vertebrates, including axial, limb and head muscles. Interestingly, we find that Rbm24 protein starts to accumulate in MyoD-positive myoblasts and is transiently expressed at the onset of muscle cell differentiation. It accumulates in myotomal and limb myogenic cells, but not in Pax3-positive progenitor cells. Rbm24 expression is under the direct regulation by MyoD, as demonstrated by in vivo chromatin immunoprecipitation assay. Using morpholino knockdown approach, we further show that Rbm24 is required for somitic myogenic progenitor cells to differentiate into muscle cells during chick somitic myogenesis. Altogether, these results highlight Rbm24 as a novel key regulator of the myogenic differentiation program during vertebrate development.
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMR 7622, Laboratory of Developmental Biology, Paris F-75005, France; CNRS, UMR 7622, Laboratory of Developmental Biology, Paris F-75005, France.
authors.forename: Raphaëlle
authors.initials: R
authors.lastname: Grifone
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMR 7622, Laboratory of Developmental Biology, Paris F-75005, France; CNRS, UMR 7622, Laboratory of Developmental Biology, Paris F-75005, France.
authors.forename: Xin
authors.initials: X
authors.lastname: Xie
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMR 7622, Laboratory of Developmental Biology, Paris F-75005, France; CNRS, UMR 7622, Laboratory of Developmental Biology, Paris F-75005, France.
authors.forename: Adeline
authors.initials: A
authors.lastname: Bourgeois
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMR 7622, Laboratory of Developmental Biology, Paris F-75005, France; CNRS, UMR 7622, Laboratory of Developmental Biology, Paris F-75005, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Saquet
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMR 7622, Laboratory of Developmental Biology, Paris F-75005, France; CNRS, UMR 7622, Laboratory of Developmental Biology, Paris F-75005, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Duprez
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMR 7622, Laboratory of Developmental Biology, Paris F-75005, France; CNRS, UMR 7622, Laboratory of Developmental Biology, Paris F-75005, France. Electronic address: de-li.shi@upmc.fr.
authors.forename: De-Li
authors.initials: DL
authors.lastname: Shi
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002642:Chick Embryo; D047108:Embryonic Development / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D024510:Muscle Development / Q000502:physiology*; D018482:Muscle, Skeletal / Q000196:embryology* / Q000378:metabolism; D032446:Myoblasts / Q000378:metabolism*; D018006:Myogenic Regulatory Factors / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D014981:Xenopus; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D018006:Myogenic Regulatory Factors; D016601:RNA-Binding Proteins; C547648:Rbm24 protein, mouse; D029867:Xenopus Proteins
keywords: Chick; Embryonic myogenesis; Mouse; MyoD; Myogenic differentiation; Rbm24