title: Inhibition study of alanine aminotransferase enzyme using sequential online capillary electrophoresis analysis.
abstract: We report the study of several inhibitors on alanine aminotransferase (ALT) enzyme using sequential online capillary electrophoresis (CE) assay. Using metal ions (Na(+) and Mg(2+)) as example inhibitors, we show that evolution of the ALT inhibition reaction can be achieved by automatically and simultaneously monitoring the substrate consumption and product formation as a function of reaction time. The inhibition mechanism and kinetic constants of ALT inhibition with succinic acid and two traditional Chinese medicines were derived from the sequential online CE assay. Our study could provide valuable information about the inhibition reactions of ALT enzyme.
authors.affiliation: Faculty of Chemistry, Northeast Normal University, Changchun, Jilin 130024, PR China.
authors.forename: Lina
authors.initials: L
authors.lastname: Liu
authors.affiliation: Faculty of Chemistry, Northeast Normal University, Changchun, Jilin 130024, PR China.
authors.forename: Yuanfang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Faculty of Chemistry, Northeast Normal University, Changchun, Jilin 130024, PR China. Electronic address: yangl330@nenu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
mesh_terms: D000409:Alanine / Q000378:metabolism*; D000410:Alanine Transaminase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D057075:Enzyme Assays / Q000295:instrumentation* / Q000379:methods; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D019802:Succinic Acid / Q000494:pharmacology
chemical_list: D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors; C504928:Qingkailing; D019802:Succinic Acid; D000410:Alanine Transaminase; D000409:Alanine
keywords: Alanine aminotransferase; Capillary electrophoresis; Inhibition; Sequential analysis