title: Antioxidant properties of extract and fractions from Enteromorpha prolifera, a type of green seaweed.
abstract: The ethanol extract and its solvent subfractions, partitioned by n-hexane (HX), chloroform (CF) and ethylacetate (EA), from Enteromorpha prolifera were measured for antioxidant activities, and a structural identification of the active compound was performed using spectroscopic techniques. The CF fraction showed the most potent 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH) radical scavenging activities with strong reducing ability. The DPPH and hydroxyl radical scavenging capacities of the CF fraction were comparable to the capacities of the positive controls, BHA and α-tocopherol, at concentrations ranging from 0.25 to 1.0mg/mL. However, little correlation (r(2)=0.03-0.48) was observed between antioxidant activities and total phenolic contents of the extracts. Further fractionation and spectroscopic analysis of the CF fraction suggested that the strong antioxidant activity of the extracts from E. prolifera was because of a chlorophyll compound, pheophorbide a, rather than phenolic compounds.
authors.affiliation: Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangneung, Gangwon 210-702, Republic of Korea.
authors.forename: MyoungLae
authors.initials: M
authors.lastname: Cho
authors.affiliation: Marine Natural Products Laboratory, Korea Ocean Research and Development Institute, Ansan, Gyeonggi 426-744, Republic of Korea.
authors.forename: Hyi-Seung
authors.initials: HS
authors.lastname: Lee
authors.affiliation: Department of Food Science and Nutrition, Hallym University, Chuncheon, Gangwon 200-702, Republic of Korea.
authors.forename: Il-Jun
authors.initials: IJ
authors.lastname: Kang
authors.affiliation: Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chuncheon, Gangwon 200-702, Republic of Korea.
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
authors.affiliation: Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangneung, Gangwon 210-702, Republic of Korea. Electronic address: umyousg@gwnu.ac.kr.
authors.forename: SangGuan
authors.initials: S
authors.lastname: You
keywords: Antioxidant; DPPH; Enteromorpha prolifera; Hydroxyl radical; Pheophorbide a; Reducing power