title: Comparative physiological and proteomic analyses of poplar (Populus yunnanensis) plantlets exposed to high temperature and drought.
abstract: Plantlets of Populus yunnanensis Dode were examined in a greenhouse for 48 h to analyze their physiological and proteomic responses to sustained heat, drought, and combined heat and drought. Compared with the application of a single stress, simultaneous treatment with both stresses damaged the plantlets more heavily. The plantlets experienced two apparent response stages under sustained heat and drought. During the first stage, malondialdehyde and reactive oxygen species (ROS) contents were induced by heat, but many protective substances, including antioxidant enzymes, proline, abscisic acid (ABA), dehydrin, and small heat shock proteins (sHSPs), were also stimulated. The plants thus actively defended themselves against stress and exhibited few pathological morphological features, most likely because a new cellular homeostasis was established through the collaborative operation of physiological and proteomic responses. During the second stage, ROS homeostasis was overwhelmed by substantial ROS production and a sharp decline in antioxidant enzyme activities, while the synthesis of some protective elements, such as proline and ABA, was suppressed. As a result, photosynthetic levels in P. yunnanensis decreased sharply and buds began to die, despite continued accumulation of sHSPs and dehydrin. This study supplies important information about the effects of extreme abiotic environments on woody plants.
authors.affiliation: Key Laboratory for Plant Biodiversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China; Plant Germplasm and Genomics Center, The Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory for Plant Biodiversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China; Plant Germplasm and Genomics Center, The Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Yunqiang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Key Laboratory for Plant Biodiversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China; Plant Germplasm and Genomics Center, The Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Sun
authors.affiliation: Key Laboratory for Plant Biodiversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China; Plant Germplasm and Genomics Center, The Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Huaming
authors.initials: H
authors.lastname: Lin
authors.affiliation: Key Laboratory of Forest Genetics & Biotechnology, Nanjing Forestry University, Nanjing, China.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Grassland Science, Yunnan Agricultural University, Kunming, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ren
authors.affiliation: Key Laboratory for Plant Biodiversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China; Plant Germplasm and Genomics Center, The Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Hu
authors.affiliation: Key Laboratory for Plant Biodiversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China; Plant Germplasm and Genomics Center, The Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000222:Adaptation, Physiological; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D008315:Malondialdehyde / Q000378:metabolism; D010641:Phenotype; D010788:Photosynthesis; D018515:Plant Leaves / Q000502:physiology; D032107:Populus / Q000502:physiology*; D011392:Proline / Q000378:metabolism; D020543:Proteome; D040901:Proteomics; D017382:Reactive Oxygen Species; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D020543:Proteome; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D011392:Proline; D013482:Superoxide Dismutase