title: Intracellular retention of the extracellular domain of the (pro)renin receptor in mammalian cells.
abstract: As a component of the renin-angiotensin system, the (pro)renin receptor [(P)RR] activates prorenin along with intracellular signaling pathways. In this study, the glutathione S-transferase-fused extracellular domain of (P)RR expressed in mammalian cells was recovered in the detergent phase in detergent-based two-phase separation experiments, and intracellular localization was observed by immunocytochemistry, suggesting retention inside the cell through stable membrane association.
authors.affiliation: a The United Graduate School of Agricultural Sciences, Gifu University , Gifu , Japan.
authors.forename: Chiharu
authors.initials: C
authors.lastname: Suzuki-Nakagawa
authors.forename: Misa
authors.initials: M
authors.lastname: Nishimura
authors.forename: Masako
authors.initials: M
authors.lastname: Noda
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Iwata
authors.forename: Masaru
authors.initials: M
authors.lastname: Hattori
authors.forename: Akio
authors.initials: A
authors.lastname: Ebihara
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Suzuki
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nakagawa
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D005110:Extracellular Space / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000378:metabolism*; D012084:Renin-Angiotensin System; D000093342:Prorenin Receptor
chemical_list: D011956:Receptors, Cell Surface; D000093342:Prorenin Receptor
keywords: (pro)renin receptor; ATP6AP2; extracellular domain; glutathione S-transferase (GST); phase separation