title: Enhanced antioxidant capacity and anti-ageing biomarkers after diet micronutrient supplementation.
abstract: A growing number of studies confirm an important effect of diet, lifestyle and physical activity on health status, the ageing process and many metabolic disorders. This study focuses on the influence of a diet supplement, NucleVital®Q10 Complex, on parameters related to redox homeostasis and ageing. An experimental group of 66 healthy volunteer women aged 35-55 supplemented their diet for 12 weeks with the complex, which contained omega-3 acids (1350 mg/day), ubiquinone (300 mg/day), astaxanthin (15 mg/day), lycopene (45 mg/day), lutein palmitate (30 mg/day), zeaxanthine palmitate (6 mg/day), L-selenomethionine (330 mg/day), cholecalciferol (30 µg/day) and α-tocopherol (45 mg/day). We found that NucleVital®Q10 Complex supplementation significantly increased total antioxidant capacity of plasma and activity of erythrocyte superoxide dismutase, with slight effects on oxidative stress biomarkers in erythrocytes; MDA and 4-hydroxyalkene levels. Apart from the observed antioxidative effects, the tested supplement also showed anti-ageing activity. Analysis of expression of SIRT1 and 2 in PBMCs showed significant changes for both genes on a mRNA level. The level of telomerase was also increased by more than 25%, although the length of lymphocyte telomeres, determined by RT-PCR, remained unchanged. Our results demonstrate beneficial effects concerning the antioxidant potential of plasma as well as biomarkers related to ageing even after short term supplementation of diet with NucleVital®Q10 Complex.
authors.affiliation: Department of Molecular Biophysics, University of Lodz, Pomorska 141/143, Lodz 90-236, Poland.
authors.forename: Aneta
authors.initials: A
authors.lastname: Balcerczyk
authors.affiliation: Department of Molecular Biophysics, University of Lodz, Pomorska 141/143, Lodz 90-236, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Gajewska
authors.affiliation: Department of Molecular Biophysics, University of Lodz, Pomorska 141/143, Lodz 90-236, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Macierzyńska-Piotrowska
authors.affiliation: Department of Affective and Psychotic Disorders, Medical University of Lodz, Czechoslowacka 8/10, Lodz 92-216, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Pawelczyk
authors.affiliation: Department of Molecular Biophysics, University of Lodz, Pomorska 141/143, Lodz 90-236, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bartosz
authors.affiliation: Department of Medical Biochemistry, Medical University in Lodz, Mazowiecka 6/8, Lodz 92-215, Poland. jszemraj@csk.umed.lodz.pl.
authors.forename: Janusz
authors.initials: J
authors.lastname: Szemraj
mesh_terms: D000328:Adult; D000375:Aging / Q000187:drug effects*; D000975:Antioxidants / Q000008:administration & dosage*; D015415:Biomarkers / Q000097:blood; D019208:Brain-Derived Neurotrophic Factor / Q000097:blood; D019587:Dietary Supplements; D004338:Drug Combinations; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006706:Homeostasis; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000502:physiology; D015227:Lipid Peroxidation; D018977:Micronutrients / Q000008:administration & dosage*; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000097:blood; D059505:Telomere Homeostasis / Q000187:drug effects
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D019208:Brain-Derived Neurotrophic Factor; D004338:Drug Combinations; D018977:Micronutrients; C579455:BDNF protein, human; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase