title: Impact of dietary polydextrose fiber on the human gut metabolome.
abstract: The aim of the present study was to elucidate the impact of polydextrose PDX an soluble fiber, on the human fecal metabolome by high-resolution nuclear magnetic resonance (NMR) spectroscopy-based metabolomics in a dietary intervention study (n = 12). Principal component analysis (PCA) revealed a strong effect of PDX consumption on the fecal metabolome, which could be mainly ascribed to the presence of undigested fiber and oligosaccharides formed from partial degradation of PDX. Our results demonstrate that NMR-based metabolomics is a useful technique for metabolite profiling of feces and for testing compliance to dietary fiber intake in such trials. In addition, novel associations between PDX and the levels of the fecal metabolites acetate and propionate could be identified. The establishment of a correlation between the fecal metabolome and levels of Bifidobacterium (R(2) = 0.66) and Bacteroides (R(2) = 0.46) demonstrates the potential of NMR-based metabolomics to elucidate metabolic activity of bacteria in the gut.
authors.affiliation: Department of Food Science, Aarhus University , Kirstinebjergvej 10, DK-5792 Aarslev, Denmark.
authors.forename: Santosh
authors.initials: S
authors.lastname: Lamichhane
authors.forename: Christian C
authors.initials: CC
authors.lastname: Yde
authors.forename: Sofia
authors.initials: S
authors.lastname: Forssten
authors.forename: Arthur C
authors.initials: AC
authors.lastname: Ouwehand
authors.forename: Markku
authors.initials: M
authors.lastname: Saarinen
authors.forename: Henrik Max
authors.initials: HM
authors.lastname: Jensen
authors.forename: Glenn R
authors.initials: GR
authors.lastname: Gibson
authors.forename: Robert
authors.initials: R
authors.lastname: Rastall
authors.forename: Francesca
authors.initials: F
authors.lastname: Fava
authors.forename: Hanne Christine
authors.initials: HC
authors.lastname: Bertram
mesh_terms: D000293:Adolescent; D000328:Adult; D004043:Dietary Fiber / Q000494:pharmacology*; D005243:Feces; D005260:Female; D041981:Gastrointestinal Tract / Q000187:drug effects* / Q000378:metabolism* / Q000382:microbiology; D005936:Glucans / Q000494:pharmacology*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055442:Metabolome; D055432:Metabolomics; D008875:Middle Aged; D015999:Multivariate Analysis; D055815:Young Adult
chemical_list: D004043:Dietary Fiber; D005936:Glucans; C033375:polydextrose
keywords: fiber; gut flora−host interactions; gut microbiota; metabolomics; microbial metabolic activity; nuclear magnetic resonances