title: Synergistic effect of Se-methylselenocysteine and vitamin E in ameliorating the acute ethanol-induced oxidative damage in rat.
abstract: The present study was conduced to investigate the synergistic effects of combined treatments with Se-methylselenocysteine (SeMSC) and vitamin E (Vit E) in reversing oxidative stress induced by ethanol in serum and different tissues of rats. Sixty female rats were randomly divided into six groups for 30 days' consecutive pretreatments as followed: control (I), physiological saline (II), 2.8μgkg(-1) Se as SeMSC (III), 2.8μgkg(-1) Se as sodium selenite (Na2SeO3, IV), 5mgkg(-1) α-tocopherol as α-tocopherol acetate (Vit E, V), 5mgkg(-1) α-tocopherol as α-tocopherol acetate and 2.8μgkg(-1) Se as SeMSC (VI). All animals in groups II-VI were treated by ethanol treatment to cause oxidative stress. After 6h of ethanol treatment, the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), the contents of total antioxidant capacity (T-AOC), malondialdehyde (MDA), glutathione (GSH) and carbonyl protein (CP) in the serum, liver, heart and kidney were measured. The result showed that the individual SeSMC, Na2SeO3 and vitamin E could effectively increase the SOD, T-AOC, GSH-Px and GSH contents as well as significantly decrease the MDA and CP concentrations in the tissues of ethanol-induced rats. At the same dose on different forms of Se, SeMSC showed greater antioxidant activity than Na2SeO3. Moreover, group VI (SeMSC and α-tocopherol acetate) showed much better antioxidant activity than individual group III (SeMSC) and V (α-tocopherol acetate) due to the synergistic effect.
authors.affiliation: State Key Lab of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yao
authors.affiliation: State Key Lab of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Lab of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: lihongyan@ncu.edu.cn.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Lab of Food Science and Technology, Nanchang University, Nanchang 330047, China; Nanoscale Science and Technology Laboratory, Institute for Advanced Study, Nanchang University, Nanchang 330031, China. Electronic address: zeyuandeng@hotmail.com.
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Chuanqi Pharmaceutical Corp. Ltd, Jiangxi Povince, Nanchang 330047, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D017279:Selenocysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D017279:Selenocysteine; D014810:Vitamin E; D000431:Ethanol; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; C002979:selenomethylselenocysteine
keywords: Antioxidant activity; Na(2)SeO(3); Se-methylselenocysteine; Synergistic effect; Vitamin E