title: Confinement-Induced Enhancement of Antigen-Antibody Interactions within Binary Nanopatterns to Achieve Higher Efficiency of On-Chip Immunosensors.
abstract: By combining molecular self-assembly, nanosphere lithography and reactive ion etching, large-scale nanopatterns of antibodies are fabricated for direct application in state-of-the-art on-chip immunosensors. Using in-situ surface plasmon resonance, the patterns are studied in view of their antigen binding capacity, which shows an increase of up to 120% solely in the case of antibody confinement into the nanopatches by means of a nonfouling embedding matrix.
authors.affiliation: Frontier Research Department Bio-Nanotechnology Research Project Fujirebio, Inc. 51 Komiya-cho, Hachioji-shi, Tokyo, 192-0031 (Japan).
authors.forename: Sivashankar
authors.initials: S
authors.lastname: Krishnamoorthy
authors.forename: Michael
authors.initials: M
authors.lastname: Himmelhaus
keywords: lithography; sensors; surface patterning; surface plasmon resonance