title: Fingerprint-efficacy study of artificial Calculus bovis in quality control of Chinese materia medica.
abstract: For quality control of Chinese materia medica (CMM), an attempt on fingerprint-efficacy study of artificial Calculus bovis was developed in this work. Chemical fingerprints of artificial C. bovis samples from ten different sources were determined by UPLC-ELSD and investigated by similarity analysis and hierarchical clustering analysis. Antibacterial effects of these samples on Escherichia coli growth were measured using microcalorimetry. The fingerprint-efficacy relationship of chemical fingerprint and antibacterial effect of artificial C. bovis were established by multi-linear regression analysis. Our results showed that the sources and places of production of artificial C. bovis had some important influence on the chemical fingerprints and antibacterial effects of this CMM. These artificial C. bovis could be grouped into four clusters according to their chemical fingerprints and antibacterial effects. Compounds cholic acid, taurocholate sodium, hyodeoxycholic acid and one unknown compound might be the major effective components for quality control of this CMM. Fingerprint-efficacy study provided a powerful way and some insight for the quality control of artificial C. bovis and other CCMs.
authors.affiliation: China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, PR China; Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, PR China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Kong
authors.affiliation: China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, PR China.
authors.forename: Jiabo
authors.initials: J
authors.lastname: Wang
authors.affiliation: China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, PR China; Pharmacy College, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, PR China.
authors.forename: Qingce
authors.initials: Q
authors.lastname: Zang
authors.affiliation: China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, PR China; Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Xing
authors.affiliation: China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, PR China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Pharmacy College, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, PR China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Jin
authors.affiliation: Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, PR China.
authors.forename: Zulun
authors.initials: Z
authors.lastname: Li
authors.affiliation: China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, PR China. Electronic address: kongwj302@126.com.
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Xiao
keywords: Antibacterial effect; Artificial Calculus bovis; Chemical fingerprint; Fingerprint–efficacy; Quality control