title: Protein profile of mature soybean seeds and prepared soybean milk.
abstract: The soybean (Glycine max (L.) Merrill) is economically the most important bean in the world, providing a wide range of vegetable proteins. Soybean milk is a colloidal solution obtained as water extract from swelled and ground soybean seeds. Soybean proteins represent about 35-40% on a dry weight basis and they are receiving increasing attention with respect to their health effects. However, the soybean is a well-recognized allergenic food, and therefore, it is urgent to define its protein components responsible for the allergenicity in order to develop hypoallergenic soybean products for sensitive people. The main aim of this work was the characterization of seed and milk soybean proteome and their comparison in terms of protein content and specific proteins. Using a shotgun proteomics approach, 243 nonredundant proteins were identified in mature soybean seeds.
authors.affiliation: Dipartimento di Chimica, Università di Roma "La Sapienza" , Piazzale Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Anna Laura
authors.initials: AL
authors.lastname: Capriotti
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Caruso
authors.forename: Chiara
authors.initials: C
authors.lastname: Cavaliere
authors.forename: Roberto
authors.initials: R
authors.lastname: Samperi
authors.forename: Serena
authors.initials: S
authors.lastname: Stampachiacchiere
authors.forename: Riccardo
authors.initials: R
authors.lastname: Zenezini Chiozzi
authors.forename: Aldo
authors.initials: A
authors.lastname: Laganà
mesh_terms: D000485:Allergens / Q000032:analysis; D005916:Globulins / Q000032:analysis; D037102:Lectins / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D055314:Seed Storage Proteins / Q000032:analysis; D012639:Seeds / Q000737:chemistry*; D041942:Soy Milk / Q000737:chemistry*; D030262:Soybean Proteins / Q000032:analysis* / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry*
chemical_list: D000485:Allergens; D005916:Globulins; D037102:Lectins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C011474:glycinin
keywords: Glycine max; liquid chromatography−mass spectrometry; proteome; seed storage proteins; soy milk; soybean