title: Molecular phenotypic profiling of a Saccharomyces cerevisiae strain at the single-cell level.
abstract: Studying cell-to-cell heterogeneity requires techniques which robustly deliver reproducible results with single-cell sensitivity. Through a new fabrication method for the microarrays for mass spectrometry (MAMS) platform, we now have attained robustness and reproducibility in our single-cell level mass spectrometry measurements that allowed us to combine single-cell MAMS-based measurements from different days and samples. By combining multiple measurements, we were able to identify three co-existing phenotypes in an isogenic population of Saccharomyces cerevisiae characterized by distinctively different levels of glycolytic intermediates.
authors.affiliation: Institute of Molecular Systems Biology, ETH Zurich, CH-8093 Zurich, Switzerland.
authors.forename: A Mareike
authors.initials: AM
authors.lastname: Schmidt
authors.forename: Stephan R
authors.initials: SR
authors.lastname: Fagerer
authors.forename: Konstantins
authors.initials: K
authors.lastname: Jefimovs
authors.forename: Florian
authors.initials: F
authors.lastname: Buettner
authors.forename: Christian
authors.initials: C
authors.lastname: Marro
authors.forename: Erdem C
authors.initials: EC
authors.lastname: Siringil
authors.forename: Karl L
authors.initials: KL
authors.lastname: Boehlen
authors.forename: Martin
authors.initials: M
authors.lastname: Pabst
authors.forename: Alfredo J
authors.initials: AJ
authors.lastname: Ibáñez
mesh_terms: D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization