title: In-vitro Evaluation of Cytotoxic and Apoptogenic Properties of Sophora Pachycarpa.
abstract: Sophora pachycarpa Schrenk ex C.A.Mey. belongs to the family Fabaceae. Some species of the genus Sophora have shown to possess anti-proliferative and apoptosis-inducing activities in cancer cells. However, there is no available information addressing this effect in S. pachycarpa. Here, we investigated the cytotoxic effects of methanol extract and different fractions obtained from S. pachycarpa root on different cancer cell lines including A549, HeLa, HL-60, MCF-7, and PC3 cell lines and leukocytes as non-malignant cells. Apoptotic cells were determined using PI staining of DNA fragmentation by flow cytometry (sub-G1 peak). S. pachycarpa inhibited the growth of malignant cells in a dose-dependent manner. CH2Cl2 and EtOAc fractions showed the lowest IC50 values ranging from 6 to 50 μg/mL in various cancer cell lines. HeLa cells as the most sensitive cells were chosen for further mechanistic studies. The sub-G1 peak in flow cytometry histogram of S. pachycarpa treated HeLa cells indicates apoptotic cell death in S. pachycarpa-induced toxicity. In conclusion, S. pachycarpa exerts cytotoxic effects in different cancer cell lines in which apoptosis plays an important role. Thus, S. pachycarpa could be considered as a potential chemotherapeutic agent in cancer treatment.
authors.affiliation: Medical Toxicology Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Seyed Hadi
authors.initials: SH
authors.lastname: Mousavi
authors.affiliation: Medical Toxicology Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Mahsa
authors.initials: M
authors.lastname: Motaez
authors.affiliation: Pharmacological Research Centre of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Zamiri-Akhlaghi
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Seyed Ahmad
authors.initials: SA
authors.lastname: Emami
authors.affiliation: Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Tayarani-Najaran
keywords: Apoptosis; Cancer; Cytotoxicity; Fabaceae; Sophora pachycarpa