title: In vitro study of CaTiO3-hydroxyapatite composites for bone tissue engineering.
abstract: A biocomposite composed of hydroxyapatite (HAp) and CaTiO3 was fabricated to study the phase stability, mechanical strength, and biocompatibility for bone tissue engineering. To investigate the optimal concentrations for the biocomposite, different HAp concentrations (0%, 50%, 70%, and 100%) were mixed with CaTiO3 and sintered in a microwave furnace. X-ray diffraction patterns of CaTiO3/HAp composites indicated the phase stability of CaTiO3/HAp. Mechanical properties were characterized by Vickers hardness, Young modulus, fracture toughness, brittleness, and compressive strength. MC3T3-E1 cells were used for in vitro studies to investigate the biocompatibility of CaTiO3/HAp composites, using 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay and immunofluorescence. The in vitro studies confirmed the highest cell viability on 70HAp at 1, 3, and 7 days. Collagen Type I, osteopontin, and osteocalcin expressions were evaluated by Western blotting and a strong signal of collagen Type I and osteopontin expression was shown by cells grown on 70HAp and 100HAp. Interestingly, osteocalcin signal was found only on 70HAp at day 7. The expression of alkaline phosphatase and osteopontin confirmed that the 70HAp expressed the strongest fluorescent signal as compared with pure materials. Thus considering the biological properties, 70HAp biocomposite was found ideal for bone tissue engineering.
authors.affiliation: From the *Department of Regenerative Medicine and †Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, Cheonan, Republic of Korea.
authors.forename: Nguyen
authors.initials: N
authors.lastname: Thuy Ba Linh
authors.forename: Dibakar
authors.initials: D
authors.lastname: Mondal
authors.forename: Byong Taek
authors.initials: BT
authors.lastname: Lee
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001696:Biomechanical Phenomena; D018786:Bone Substitutes; D017610:Calcium Compounds; D049109:Cell Proliferation; D002470:Cell Survival; D024042:Collagen Type I / Q000378:metabolism; D019245:Compressive Strength; D017886:Durapatite; D055119:Elastic Modulus; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D053495:Osteopontin / Q000378:metabolism; D010087:Oxides; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry; D014025:Titanium; D014961:X-Ray Diffraction
chemical_list: D018786:Bone Substitutes; D017610:Calcium Compounds; D024042:Collagen Type I; D010087:Oxides; C506896:Spp1 protein, mouse; D015675:Osteocalcin; D053495:Osteopontin; C059910:perovskite; D017886:Durapatite; D014025:Titanium; D000469:Alkaline Phosphatase