title: Evaluation of a rapid antigen detection point-of-care test for respiratory syncytial virus and influenza in a pediatric hospitalized population in the Netherlands.
abstract: This pilot study evaluates the diagnostic performance of Sofia RSV Fluorescent Immunoassay Analyzer (FIA) and Sofia Influenza A + B FIA for rapid detection of respiratory syncytial virus and influenza A and B. Sofia had a lower-than-expected sensitivity for all viruses and a high rate of false-positive results for influenza B virus.
authors.affiliation: Department of Pediatrics, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands. Electronic address: a.h.bruning@amc.uva.nl.
authors.forename: Andrea H L
authors.initials: AH
authors.lastname: Bruning
authors.affiliation: Department of Medical Microbiology and Infection Control, VU University Medical Center, De Boelelaan 1118, 1081 HZ Amsterdam, The Netherlands.
authors.forename: Karin
authors.initials: K
authors.lastname: van Dijk
authors.affiliation: Laboratory of Clinical Virology, Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.
authors.forename: Hetty W M
authors.initials: HW
authors.lastname: van Eijk
authors.affiliation: Laboratory of Clinical Virology, Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.
authors.forename: Gerrit
authors.initials: G
authors.lastname: Koen
authors.affiliation: Department of Pediatrics, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.
authors.forename: Job B M
authors.initials: JB
authors.lastname: van Woensel
authors.affiliation: Department of Pediatrics, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.
authors.forename: Frea H
authors.initials: FH
authors.lastname: Kruisinga
authors.affiliation: Department of Pediatrics, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.
authors.forename: Dasja
authors.initials: D
authors.lastname: Pajkrt
authors.affiliation: Laboratory of Clinical Virology, Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.
authors.forename: Katja C
authors.initials: KC
authors.lastname: Wolthers
mesh_terms: D000293:Adolescent; D000956:Antigens, Viral / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D005189:False Positive Reactions; D015200:Fluoroimmunoassay / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D009980:Influenza A virus / Q000276:immunology / Q000472:pathogenicity; D009981:Influenza B virus / Q000276:immunology / Q000472:pathogenicity; D007251:Influenza, Human / Q000175:diagnosis / Q000821:virology*; D009426:Netherlands; D010865:Pilot Projects; D019095:Point-of-Care Systems; D016133:Polymerase Chain Reaction / Q000379:methods; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis* / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000276:immunology* / Q000472:pathogenicity; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000821:virology; D012680:Sensitivity and Specificity
chemical_list: D000956:Antigens, Viral
keywords: Antigen detection; Children; Point-of-care; Rapid testing; Respiratory infection