title: Screening of key antioxidant compounds of longan (Dimocarpus longan Lour.) seed extract by combining online fishing/knockout, activity evaluation, Fourier transform ion cyclotron resonance mass spectrometry, and high-performance liquid chromatography electrospray ionization mass spectrometry methods.
abstract: To figure out the key phenolic compounds accounting for the antioxidant effects of longan (Dimocarpus longan Lour.) seed extract, online fishing/knockout method, activity evaluation assays, Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS), and high-performance liquid chromatography electrospray ionization mass spectrometry (HPLC-ESI-MS) analysis were used jointly for the first time. p-Coumaric acid-glycoside, (S)-flavogallonic acid, ellagic acid derivative, and methyl-ellagic acid glucopyranoside were first identified in longan seeds. In addition, our study revealed that ellagic acid as well as its derivative and p-coumaric acid-glycoside had important contribution to the potent antioxidant activity of longan seed extract, while gallic acid, corilagin, (S)-flavogallonic acid, methyl-ellagic acid glucopyranoside, and ethyl gallate showed very little contribution to the total antioxidant activity of longan seed extract. The combining use of the online fishing/knockout method, activity evaluation assays, FT-ICR-MS, and HPLC-ESI-MS analysis is a useful and simple strategy for screening of key bioactive compounds from complex extracts.
authors.affiliation: College of Food Science and Technology, and ‡Key Laboratory of Environment Correlative Food Science, Ministry of Education, Huazhong Agricultural University , Wuhan, Hubei 430070, People's Republic of China.
authors.forename: Jinyu
authors.initials: J
authors.lastname: Chen
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Ge
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.forename: Ze
authors.initials: Z
authors.lastname: Xu
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Li
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003373:Coumaric Acids / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D017357:Cyclotrons; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005583:Fourier Analysis; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology; D005960:Glucosides / Q000032:analysis / Q000494:pharmacology; D047348:Hydrolyzable Tannins / Q000032:analysis / Q000737:chemistry; D009862:Online Systems; D011422:Propionates; D029631:Sapindaceae / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
chemical_list: C000597882:(S)-flavogallonic acid; D000975:Antioxidants; D003373:Coumaric Acids; D005960:Glucosides; D047348:Hydrolyzable Tannins; D011422:Propionates; C048734:ethyl gallate; C049096:corilagin; D005707:Gallic Acid; C495469:p-coumaric acid
keywords: FT-ICR−MS; HPLC−ESI−MS; longan seed; online knockout; phenolic compounds