title: Inhibitory effects of bee venom on Propionibacterium acnes-induced inflammatory skin disease in an animal model.
abstract: Propionibacterium acnes (P. acnes) is a major contributing factor to the inflammatory component of acne. The many prescription medications for acne allow for a large number of potential combination treatments. However, several antibiotics, apart from their antibacterial effects, exert side‑effects, such as the suppression of host inflammatory responses. Purified bee venom (BV) is a natural toxin produced by honeybees (Apis mellifera L.). BV has been widely used as a traditional medicine for various diseases. In the present study, to investigate the therapeutic effects of BV against P. acnes-induced inflammatory skin disease, P. acnes was intradermally injected into the ears of mice. After the injection, BV was applied to the skin surface of the right ear. Histological observation revealed that P. acnes induced a considerable increase in the number of infiltrated inflammatory cells. However, treatment with BV markedly reduced these reactions compared with the P. acnes-injected mice not treated with BV. Moreover, the expression levels of tumor necrosis factor (TNF)-α, and interleukin (IL)-1β were significantly reduced in the BV-treated mice compared with the untreated P. acnes-injected mice. In addition, treatment with BV significantly inhibited Toll-like receptor (TLR)2 and CD14 expression in P. acnes-injected tissue. The binding activity of nuclear factor-κB (NF-κB) and activator protein (AP)-1 was markedly suppressed following treatment with BV. The results from our study, using an animal model, indicate that BV exerts an inhibitory effect on inflammatory skin diseases. In conclusion, our data indicate that BV has potential for use as an anti-acne agent and may be useful in the pharmaceutical and cosmetics industries.
authors.affiliation: Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu, Republic of Korea.
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: An
authors.affiliation: Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu, Republic of Korea.
authors.forename: Woo-Ram
authors.initials: WR
authors.lastname: Lee
authors.affiliation: Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu, Republic of Korea.
authors.forename: Kyung-Hyun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu, Republic of Korea.
authors.forename: Jung-Yeon
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu, Republic of Korea.
authors.forename: Sun-Jae
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biology, National Institute of Agricultural Science and Technology, Wanju, Republic of Korea.
authors.forename: Sang-Mi
authors.initials: SM
authors.lastname: Han
authors.affiliation: Department of Agricultural Biology, National Institute of Agricultural Science and Technology, Wanju, Republic of Korea.
authors.forename: Kwang-Gill
authors.initials: KG
authors.lastname: Lee
authors.affiliation: Department of Immunology, College of Medicine, Catholic University of Daegu, Daegu, Republic of Korea.
authors.forename: Chong-Kee
authors.initials: CK
authors.lastname: Lee
authors.affiliation: Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu, Republic of Korea.
authors.forename: Kwan-Kyu
authors.initials: KK
authors.lastname: Park
mesh_terms: D000152:Acne Vulgaris / Q000382:microbiology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001514:Bee Venoms / Q000494:pharmacology*; D001516:Bees; D004195:Disease Models, Animal; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D011425:Propionibacterium acnes / Q000187:drug effects*; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001514:Bee Venoms; D053583:Interleukin-1beta; D018950:Lipopolysaccharide Receptors; D016328:NF-kappa B; C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2; D018808:Transcription Factor AP-1; D014409:Tumor Necrosis Factor-alpha