title: In situ polymerization of monolith based on poly(Triallyl Isocyanurate-co-trimethylolpropane triacrylate) and its application in high-performance liquid chromatography.
abstract: A novel organic monolithic stationary phase was prepared for high-performance liquid chromatography (HPLC) by in situ copolymerization. In which, triallyl isocyanurate (TAIC) and trimethylolpropane triacrylate (TMPTA) in a binary porogenic solvent consisting of polyethylene glycol 200 and 1, 2-propanediol were used. The resultant monoliths with different column properties (e.g., morphology and pressure) were optimized by adjusting the ratio of TMPTA/TAIC and the composition of porogenic solvent. The resulting poly(TAIC-co-TMPTA) monolith showed a relatively homogeneous structure, good permeability and mechanical stability. The chemical group of the monolith was assayed by the infrared spectra method, the morphology of monolithic material was studied by scanning electron microscopy and the pore size distribution was determined by a mercury porosimeter. A series of small molecules were used to evaluate the column performance in terms of hydrophobic mode. At an optimized flow rate of 1.0 mL min(-1), the theoretical plate number of analyte was >15,000 plates m(-1). These applications demonstrated that the monoliths could be successfully used as the stationary phase in conjunction with HPLC to separate small molecules from the mixture.
authors.affiliation: College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Baoding 071002, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhong
authors.affiliation: College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Baoding 071002, China.
authors.forename: Ligai
authors.initials: L
authors.lastname: Bai
authors.affiliation: College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Baoding 071002, China.
authors.forename: Junxiao
authors.initials: J
authors.lastname: Qin
authors.affiliation: College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Baoding 071002, China.
authors.forename: Jiafei
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Baoding 071002, China.
authors.forename: Mengbei
authors.initials: M
authors.lastname: Hao
authors.affiliation: College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Baoding 071002, China bailigai@163.com.
authors.forename: Gengliang
authors.initials: G
authors.lastname: Yang
mesh_terms: D000179:Acrylates / Q000138:chemical synthesis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D057927:Hydrophobic and Hydrophilic Interactions; D008956:Models, Chemical; D058105:Polymerization; D016062:Porosity; D015203:Reproducibility of Results
chemical_list: D000179:Acrylates; C000589787:poly(triallyl isocyanurate-co-trimethylolpropane triacrylate)