title: Depletion of the RNA-binding protein RBP33 results in increased expression of silenced RNA polymerase II transcripts in Trypanosoma brucei.
abstract: We have characterized the RNA-binding protein RBP33 in Trypanosoma brucei, and found that it localizes to the nucleus and is essential for viability. The subset of RNAs bound to RBP33 was determined by immunoprecipitation of ribonucleoprotein complexes followed by deep sequencing. Most RBP33-bound transcripts are predicted to be non-coding. Among these, over one-third are located close to the end of transcriptional units (TUs) or have an antisense orientation within a TU. Depletion of RBP33 resulted in an increase in the level of RNAs derived from regions that are normally silenced, such as strand-switch regions, retroposon and repeat sequences. Our work provides the first example of an RNA-binding protein involved in the regulation of gene silencing in trypanosomes.
authors.affiliation: Instituto de Parasitología y Biomedicina "López-Neyra", IPBLN-CSIC, Armilla, Granada, Spain.
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Fernández-Moya
authors.affiliation: Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Mark
authors.initials: M
authors.lastname: Carrington
authors.affiliation: Instituto de Parasitología y Biomedicina "López-Neyra", IPBLN-CSIC, Armilla, Granada, Spain.
authors.forename: Antonio M
authors.initials: AM
authors.lastname: Estévez
mesh_terms: D000818:Animals; D002417:Cattle; D020868:Gene Silencing; D059014:High-Throughput Nucleotide Sequencing; D015800:Protozoan Proteins / Q000235:genetics*; D034622:RNA Interference; D012319:RNA Polymerase II / Q000096:biosynthesis* / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics*; D014158:Transcription, Genetic; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000472:pathogenicity; D014354:Trypanosomiasis, Bovine / Q000235:genetics / Q000469:parasitology*
chemical_list: D015800:Protozoan Proteins; D016601:RNA-Binding Proteins; D012319:RNA Polymerase II