title: [Purification of superoxide dismutase from blood erythrocyte by nano magnetic chitosan microspheres].
abstract: Nano magnetic microspheres prepared by chitosan and poly acylic acid were applied to purifying superoxide dismutase from blood erythrocyte. Chitosan-polyacyilc acid graft copolymer was synthesized by free radical graft copolymerization with potassium persulfate as inititator. To prepare Fe3O4 magnetic fluids with chemical coprecipitation, chitosan-polyacylic nano magnetic microspheres were prepared with glutaraldehyde as crosslinking agent. Structure of nano magnetic microspheres was detected by FT-IR spectrometer. Particle size and morphology were characterized by JEM-4000EX technology. Chitosan-polyacylic nanometer microspheres have good paticle cize distribution, magnetic responsiveness and protein adsoption. Activity, product yield and activity recovery of SOD after purification reached 6 727 U/mg, 21.1%, and 85.7% respectively. Purification of blood superoxide dismutase by chistosan-polyacylic acid microspheres has its renewable and feasible nature.
authors.forename: Baoquan
authors.initials: B
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Ping
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Yongzhou
authors.initials: Y
authors.lastname: Zhang
authors.forename: Cui
authors.initials: C
authors.lastname: Liu
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Pang
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D004912:Erythrocytes / Q000201:enzymology*; D005976:Glutaral / Q000737:chemistry; D008280:Magnetics; D008863:Microspheres; D011108:Polymers; D017550:Spectroscopy, Fourier Transform Infrared; D013482:Superoxide Dismutase / Q000302:isolation & purification*
chemical_list: D011108:Polymers; D048271:Chitosan; D013482:Superoxide Dismutase; D005976:Glutaral