title: Preparation and in vivo efficient anti-infection property of GTR/GBR implant made by metronidazole loaded electrospun polycaprolactone nanofiber membrane.
abstract: Infection is the major reason of GTR/GBR membrane failure in clinical application. In this work, we developed GTR/GBR nanofiber membranes with localized drug delivery function to prevent infection. Metronidazole (MNA), an antibiotic, was successfully incorporated into electrospun polycaprolactone (PCL) nanofibers at different concentrations (0, 1, 5, 10, 20, 30, and 40 wt% polymer). To obtain the optimum anti-infection membrane, we systematically investigated the physical-chemical and mechanical properties of the nanofiber membranes with different drug contents. The interaction between PCL and MNA was identified by molecular dynamics simulation. MNA released in a controlled, sustained manner over 2 weeks and the antibacterial activity of the released MNA remained. The incorporation of MNA improved the hydrophilicity and in vitro biodegradation rate of PCL nanofibers. The nanofiber membranes allowed cells to adhere to and proliferate on them and showed excellent barrier function. The membrane loaded with 30% MNA had the best comprehensive properties. Analysis of subcutaneous implants demonstrated that MNA-loaded nanofibers evoked a less severe inflammatory response than pure PCL nanofibers. These results demonstrate the potential of MNA-loaded nanofiber membranes as GTR/GBR membrane with antibacterial and anti-inflammatory function for extensive biomedical applications.
authors.affiliation: Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Xue
authors.affiliation: Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.affiliation: State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Yuzhao
authors.initials: Y
authors.lastname: Niu
authors.affiliation: DaBeiNong Group (DBN) Research Center for Animal Medicine, Beijing 100195, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.affiliation: Centre for Biomaterials and Tissue Engineering, University of Sheffield, Sheffield, South Yorkshire S3 7HQ, UK.
authors.forename: Aileen
authors.initials: A
authors.lastname: Crawford
authors.affiliation: School of Engineering, Design & Technology, Bradford University, Bradford, West Yorkshire BD7 1DP, UK.
authors.forename: Phil
authors.initials: P
authors.lastname: Coates
authors.affiliation: Laboratory of Bone Tissue Engineering of Beijing Research Institute of Traumatology and Orthopaedics, Beijing 100035, PR China.
authors.forename: Dafu
authors.initials: D
authors.lastname: Chen
authors.affiliation: Laboratory of Bone Tissue Engineering of Beijing Research Institute of Traumatology and Orthopaedics, Beijing 100035, PR China. Electronic address: sharell@126.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Shi
authors.affiliation: Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, PR China; State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: zhanglq@mail.buct.edu.cn.
authors.forename: Liqun
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D001861:Bone Regeneration; D002209:Caprolactam / Q000737:chemistry*; D049109:Cell Proliferation; D004305:Dose-Response Relationship, Drug; D016503:Drug Delivery Systems; D065546:Drug Liberation; D005347:Fibroblasts / Q000187:drug effects; D048091:Guided Tissue Regeneration / Q000379:methods*; D008297:Male; D008795:Metronidazole / Q000008:administration & dosage / Q000737:chemistry*; D008828:Microbiological Techniques; D057139:Nanofibers / Q000737:chemistry; D019736:Prostheses and Implants; D011817:Rabbits
chemical_list: D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D008795:Metronidazole; D002209:Caprolactam; C000362:capron
keywords: Anti-infection; Controlled delivery; Electrospinning; Guided tissue regeneration; Metronidazole; PCL