title: Rapid-scan EPR of immobilized nitroxides.
abstract: X-band electron paramagnetic resonance spectra of immobilized nitroxides were obtained by rapid scan at 293 K. Scan widths were 155 G with 13.4 kHz scan frequency for (14)N-perdeuterated tempone and for T4 lysozyme doubly spin labeled with an iodoacetamide spirocyclohexyl nitroxide and 100 G with 20.9 kHz scan frequency for (15)N-perdeuterated tempone. These wide scans were made possible by modifications to our rapid-scan driver, scan coils made of Litz wire, and the placement of highly conducting aluminum plates on the poles of a Bruker 10″ magnet to reduce resistive losses in the magnet pole faces. For the same data acquisition time, the signal-to-noise for the rapid-scan absorption spectra was about an order of magnitude higher than for continuous wave first-derivative spectra recorded with modulation amplitudes that do not broaden the lineshapes.
authors.affiliation: Department of Chemistry and Biochemistry, University of Denver, Denver, CO 80208, USA.
authors.forename: Zhelin
authors.initials: Z
authors.lastname: Yu
authors.affiliation: School of Engineering and Computer Science, University of Denver, Denver, CO 80208, USA.
authors.forename: Richard W
authors.initials: RW
authors.lastname: Quine
authors.affiliation: School of Engineering and Computer Science, University of Denver, Denver, CO 80208, USA.
authors.forename: George A
authors.initials: GA
authors.lastname: Rinard
authors.affiliation: Department of Chemistry and Biochemistry, University of Denver, Denver, CO 80208, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Tseitlin
authors.affiliation: Department of Chemistry and Biochemistry, University of Denver, Denver, CO 80208, USA.
authors.forename: Hanan
authors.initials: H
authors.lastname: Elajaili
authors.affiliation: Department of Chemistry and Biochemistry, University of Denver, Denver, CO 80208, USA.
authors.forename: Velavan
authors.initials: V
authors.lastname: Kathirvelu
authors.affiliation: Department of Chemistry, University of Nebraska, Lincoln, NE 68588-0304, USA.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Clouston
authors.affiliation: Department of Chemistry, University of Nebraska, Lincoln, NE 68588-0304, USA.
authors.forename: Przemysław J
authors.initials: PJ
authors.lastname: Boratyński
authors.affiliation: Department of Chemistry, University of Nebraska, Lincoln, NE 68588-0304, USA.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Rajca
authors.affiliation: Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Stein
authors.affiliation: Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
authors.forename: Hassane
authors.initials: H
authors.lastname: Mchaourab
authors.affiliation: Department of Chemistry and Biochemistry, University of Denver, Denver, CO 80208, USA.
authors.forename: Sandra S
authors.initials: SS
authors.lastname: Eaton
authors.affiliation: Department of Chemistry and Biochemistry, University of Denver, Denver, CO 80208, USA. Electronic address: geaton@du.edu.
authors.forename: Gareth R
authors.initials: GR
authors.lastname: Eaton
mesh_terms: D003903:Deuterium; D004574:Electromagnetic Fields; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D007202:Indicators and Reagents; D009113:Muramidase / Q000737:chemistry; D009589:Nitrogen Oxides / Q000737:chemistry*; D009590:Nitrogen Radioisotopes; D013113:Spin Labels; D014216:Triacetoneamine-N-Oxyl / Q000737:chemistry
chemical_list: D007202:Indicators and Reagents; D009589:Nitrogen Oxides; D009590:Nitrogen Radioisotopes; D013113:Spin Labels; D014216:Triacetoneamine-N-Oxyl; D003903:Deuterium; D009113:Muramidase
keywords: Immobilized nitroxide; Litz wire coils; Rapid scan EPR; Sucrose octaacetate glass; Trehalose glass