title: Preparation of self-assembled nanoparticles of chitosan oligosaccharide-graft-polycaprolactone as a carrier of bovine serum albumin drug.
abstract: Chitosan oligosaccharides graft polycaprolactone copolymer (PHCSO-g-PCL) has been synthesized via initiating the polymerization of e-caprolactone (CL) monomer through an amino group protection route using phthaloyl chitosan oligosaccharide (PHCSO) as intermediate. The grafting reaction was carried out in Pyridine at 120 °C with the hydroxyl group of the chitosan oligosaccharide (CSO) as initiator and the tin 2-ethylhexanoate (Sn (Oct)2) as catalyst. The PHCSO-g-PCL nanoparticles with and without bovine serum albumin (BSA) drug were prepared through the self-assembled approach in Dimethylformamide (DMF) organic solvents. PHCSO-PCL copolymer was investigated by Fourier transform infrared spectroscopy (FTIR), 1H NMR spectrum and scanning electron microscopy (SEM). The physicochemical properties of the hydrophobized PHCSO-g-PCL nanoparticles were characterized by fluorescence spectroscopy and dynamic light scattering (DLS). The results of DLS showed that the hydrodynamic diameters and particle size distribution with various concentrations of PHCSO-g-PCL nanoparticles were from 69.82 nm to 195.9 nm with a narrow polydispersity factor of 0.212 to 0.172. The results of DLS also showed that the hydrodynamic diameters and particle size distribution of PHCSO-g-PCL (5 mg/ml) nanoparticles without and with BSA drug (0.4 mg/ml) were from 168.44 nm to 200.7 nm. The polydispersity factor was from 0.119 to 0.159.
authors.affiliation: School of Petrochemical Engineering, Shenyang University of Technology, Liaoyang 111003, P.R. China.
authors.forename: Fenghong
authors.initials: F
authors.lastname: Li
authors.affiliation: School of Petrochemical Engineering, Shenyang University of Technology, Liaoyang 111003, P.R. China.
authors.forename: Xinrui
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Petrochemical Engineering, Shenyang University of Technology, Liaoyang 111003, P.R. China.
authors.forename: Hongyi
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Petrochemical Engineering, Shenyang University of Technology, Liaoyang 111003, P.R. China.
authors.forename: Liying
authors.initials: L
authors.lastname: Xiang
authors.affiliation: School of Petrochemical Engineering, Shenyang University of Technology, Liaoyang 111003, P.R. China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Chen
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D004058:Diffusion; D008422:Materials Testing; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D009844:Oligosaccharides / Q000737:chemistry*; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage* / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D053769:Nanocapsules; D009844:Oligosaccharides; D011091:Polyesters; C016240:polycaprolactone; D012710:Serum Albumin, Bovine; D048271:Chitosan
keywords: Chitosan oligosaccharide; bovine serum albumin; polycaprolactone; preparation; self-assembled nanoparticles