title: PARP-1 rs3219073 polymorphism may contribute to susceptibility to lung cancer.
abstract: OBJECTIVE
  To investigate the relationship between the PARP-1 rs3219073 C>G polymorphism and susceptibility to lung cancer in Chinese people.
  
  
  METHODS
  In accordance with the case-control study principle, 645 of the patients had histologically recognized primary lung cancer, among them 240 had squamous carcinoma, 217 had adenocarcinoma, and 188 had small-cell lung cancer. The control group consisted of 643 healthy subjects who had received a physical examination. Extracts of peripheral blood were taken from all subjects, and genomic DNA was extracted by the phenol-chloroform method.
  
  
  RESULTS
  After adjusting for age and smoking status, the results show significant association between genetic variations in the rs3219073 C/C genotype and an increased risk of lung cancer (p=0.045, odds ratio [OR]=0.625). After combining C/G, G/G is still statistically significant (p=0.042, OR=0.637). Hierarchical analysis found that the number of subjects with a G/G genotype in the adenocarcinoma group is lower than in the control group (p=0.015, OR=0.543). After combining C/G, G/G is still statistically significant (p=0.027, OR=0.595). After correcting for age and smoking status, the group with C/G genotype and the group with G/G genotype both appear to have a reduced risk for lung cancer compared with the control group (p=0.045, OR=0.566; p=0.013, OR=0.489). The combination of C/G and G/G displays a more statistically significant difference (p=0.018, OR=0.528).
  
  
  CONCLUSIONS
  The study found that PARP-1 rs3219073 C>G polymorphism is indeed associated with lung cancer susceptibility. The carriers of G alleles may have reduced risk of lung cancer, especially adenocarcinoma.
authors.affiliation: 1 Department of Radiation Oncology, The Fourth Affiliated Hospital of China Medical University , Shenyang, People's Republic of China .
authors.forename: He-Tong
authors.initials: HT
authors.lastname: Wang
authors.forename: Ya
authors.initials: Y
authors.lastname: Gao
authors.forename: Yu-Xia
authors.initials: YX
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Yu
authors.forename: Tian-Lu
authors.initials: TL
authors.lastname: Wang
authors.forename: Lu
authors.initials: L
authors.lastname: Bai
authors.forename: Yan-Zhi
authors.initials: YZ
authors.lastname: Chen
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Zhang
authors.forename: Bao-Sen
authors.initials: BS
authors.lastname: Zhou
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Qu
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000230:Adenocarcinoma / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000368:Aged; D000483:Alleles; D001482:Base Composition; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008175:Lung Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D009363:Neoplasm Proteins; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases