title: Detoxification of rats subjected to nickel chloride by a biomaterial-based carbonated orthophosphate.
abstract: Recently, the therapeutic approaches of the detoxification against the metals (nickel) in the body are the use of biomaterials such as carbonated hydroxyapatite. The aim of this study is therefore to analyze the physiological and physicochemical parameters of strain white rats "Wistar" receiving nickel chloride and to study the protective associative of apatite against adverse effects of this metal, and this in comparison with control rats. Our results showed that the nickel induced in rats an oxidative stress objectified by elevated levels of thiobarbituric acid-reactive substances and conjugated dienes associated with inhibition of the activity of the antioxidant defense system such as glutathione peroxidase, superoxide dismutase and catalase in the liver, kidney, spleen and erythrocyte. Disorders balances of ferric, phosphocalcic, a renal failure and a liver toxicity were observed in rats exposed to nickel. As well as a significant increase in the rate of nickel in the bones and microcytic anemia was revealed. However, the implantation of carbonated hydroxyapatite in capsule form protects rats intoxicated by the nickel against the toxic effects of this metal by lowering the levels of markers of lipid peroxidation and improving the activities of defense enzymes. Our implantation technique is effective to correct ferric balance and phosphocalcic equilibrium, to protect liver and kidney function, to reduce the rate of bone nickel and to correct anemia. They clearly explain the beneficial and protective of our biomaterial which aims the detoxification of rats receiving nickel by substituting cationic (Ca(2+) by Ni(2+)) and anionic (OH(-) by Cl(-)) confirmed by physicochemical characterization like the IR spectroscopy and X-ray diffraction. These techniques have shown on the one hand a duplication of OH(-) bands (IR) and on the other hand the increase of the volume of the apatite cell after these substitutions (X-ray diffraction).
authors.affiliation: Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax, University of Sfax, P.O. Box 95, 3000 Sfax, Tunisia; Laboratory of Sciences Material and environment, Faculty of Sciences of Sfax, B.P. 1171, 3000 Sfax, Tunisia; University of Rennes 1, UMR CNRS 6226, campus de Beaulieu, 35042 Rennes, France.
authors.forename: S
authors.initials: S
authors.lastname: Boulila
authors.affiliation: Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax, University of Sfax, P.O. Box 95, 3000 Sfax, Tunisia.
authors.forename: A
authors.initials: A
authors.lastname: El Feki
authors.affiliation: University of Rennes 1, UMR CNRS 6226, campus de Beaulieu, 35042 Rennes, France.
authors.forename: H
authors.initials: H
authors.lastname: Oudadesse
authors.affiliation: Laboratory of Hematology, University Hospital Habib Bourguiba, Avenue El Fardous, 3029 Sfax, Tunisia.
authors.forename: C
authors.initials: C
authors.lastname: Kallel
authors.affiliation: Laboratory of Sciences Material and environment, Faculty of Sciences of Sfax, B.P. 1171, 3000 Sfax, Tunisia. Electronic address: Hafedelfeki@yahoo.com.
authors.forename: H
authors.initials: H
authors.lastname: El Feki
mesh_terms: D000818:Animals; D000931:Antidotes / Q000008:administration & dosage / Q000627:therapeutic use*; D001031:Apatites / Q000627:therapeutic use*; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000627:therapeutic use*; D001842:Bone and Bones / Q000737:chemistry / Q000378:metabolism; D008297:Male; D009532:Nickel / Q000493:pharmacokinetics / Q000506:poisoning*; D018384:Oxidative Stress / Q000187:drug effects; D010710:Phosphates / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000931:Antidotes; D001031:Apatites; D001672:Biocompatible Materials; D010710:Phosphates; C022838:nickel chloride; D009532:Nickel
keywords: Carbonated hydroxyapatite; Detoxification; Détoxification; Hydroxyapatite carbonatée; Nickel; Substitution