title: Virus-host interactions in persistently FMDV-infected cells derived from bovine pharynx.
abstract: Foot-and-mouth disease virus (FMDV) produces a disease in cattle characterized by vesicular lesions and a persistent infection with asymptomatic low-level production of virus in pharyngeal tissues. Here we describe the establishment of a persistently infected primary cell culture derived from bovine pharynx tissue (PBPT) infected with FMDV serotype O1 Manisa, where surviving cells were serially passed until a persistently infected culture was generated. Characterization of the persistent virus demonstrated changes in its plaque size, ability to grow in different cell lines, and change in the use of integrins as receptors, when compared with the parental virus. These results demonstrate the establishment of persistently infected PBPT cell cultures where co-adaptation has taken place between the virus and host cells. This in vitro model for FMDV persistence may help further understanding of the molecular mechanisms of the cattle carrier state.
authors.affiliation: Foreign Animal Disease Research Unit, United States Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, P.O. Box 848, Greenport, NY 11944, USA; Department of Pathobiology and Veterinary Science, University of Connecticut at Storrs, Storrs, CT 06269, USA.
authors.forename: V
authors.initials: V
authors.lastname: O'Donnell
authors.affiliation: Foreign Animal Disease Research Unit, United States Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, P.O. Box 848, Greenport, NY 11944, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Pacheco
authors.affiliation: Foreign Animal Disease Research Unit, United States Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, P.O. Box 848, Greenport, NY 11944, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Larocco
authors.affiliation: Foreign Animal Disease Research Unit, United States Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, P.O. Box 848, Greenport, NY 11944, USA.
authors.forename: D P
authors.initials: DP
authors.lastname: Gladue
authors.affiliation: Foreign Animal Disease Research Unit, United States Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, P.O. Box 848, Greenport, NY 11944, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Pauszek
authors.affiliation: Foreign Animal Disease Research Unit, United States Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, P.O. Box 848, Greenport, NY 11944, USA.
authors.forename: G
authors.initials: G
authors.lastname: Smoliga
authors.affiliation: Foreign Animal Disease Research Unit, United States Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, P.O. Box 848, Greenport, NY 11944, USA.
authors.forename: P W
authors.initials: PW
authors.lastname: Krug
authors.affiliation: Foreign Animal Disease Research Unit, United States Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, P.O. Box 848, Greenport, NY 11944, USA.
authors.forename: B
authors.initials: B
authors.lastname: Baxt
authors.affiliation: Foreign Animal Disease Research Unit, United States Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, P.O. Box 848, Greenport, NY 11944, USA.
authors.forename: M V
authors.initials: MV
authors.lastname: Borca
authors.affiliation: Foreign Animal Disease Research Unit, United States Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, P.O. Box 848, Greenport, NY 11944, USA.
authors.forename: L
authors.initials: L
authors.lastname: Rodriguez
mesh_terms: D000818:Animals; D002417:Cattle; D002477:Cells; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D029544:Foot-and-Mouth Disease Virus / Q000502:physiology*; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D010614:Pharynx / Q000166:cytology*; D013997:Time Factors; D014779:Virus Replication
chemical_list: D016207:Cytokines
keywords: Bovine pharynx; FMDV; Persistence; Picornavirus