title: Chemical constituents and activities of the essential oil from Myristica fragrans against cigarette beetle Lasioderma serricorne.
abstract: Essential oil extracted from nutmeg seeds (Myristica fragrans Houtt.) by hydrodistillation was subjected to GC/MS and GC analysis. A total of 27 constituents were identified, of which eugenol (19.9%), methylisoeugenol (16.8%), methyleugenol (16.7%), sabinene (11.8%), and terpinen-4-ol (8.5%) were the major components. The essential oil was tested against Lasioderma serricorne for insecticidal and repellent activity, the LD50 value at the end of 24 h exposure period was 19.3 μg/adult. Six active compounds were isolated by bioassay-guided fractionation. They were identified as eugenol (1), methyleugenol (2), methylisoeugenol (3), elemicin (4), myristicin (5), and safrole (6). Among these isolates, 4 showed the strongest contact toxicity against L. serricorne adults with an LD50 value of 9.8 μg/adult. Repellency of crude oil and active compounds were also determined. Compounds 1, 2, 4, and 5 were strongly repellent against the cigarette beetle and exhibited the same level of repellency compared with the positive control, DEET. The results indicate that the essential oil of M. fragrans and its active constituents have potential for development as natural insecticides and repellents to control L. serricorne.
authors.affiliation: State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University, Beijing 100875, P. R. China (phone: +86-10-62208022). dushushan@bnu.edu.cn.
authors.forename: Shu-Shan
authors.initials: SS
authors.lastname: Du
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.forename: Cheng-Fang
authors.initials: CF
authors.lastname: Wang
authors.forename: Chun-Xue
authors.initials: CX
authors.lastname: You
authors.forename: Zhu-Feng
authors.initials: ZF
authors.lastname: Geng
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Guo
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Deng
authors.forename: Zhi-Long
authors.initials: ZL
authors.lastname: Liu
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D001517:Coleoptera / Q000187:drug effects*; D008401:Gas Chromatography-Mass Spectrometry; D026324:Myristicaceae / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D009822:Oils, Volatile
keywords: Contact toxicity; Essential oils; Lasioderma serricorne; Myristica fragrans; Repellent activity