title: Effects of enzymatically modified starch on the encapsulation efficiency and stability of water-in-oil-in-water emulsions.
abstract: The present study was performed to investigate the possibility of using 4-α-glucanotransferase (4αGTase)-treated starch in W/O/W emulsions to increase their encapsulation efficiency (EE) and stability. Emulsions were prepared using soybean oil, polyglycerol polyricinoleate (PGPR), 4αGTase-treated starch and Tween 20. The mean diameter of W/O/W droplets ranged from 4 to 10μm depending on the sonication time. When the dye was loaded in the internal water phase, the emulsion prepared by sonication for 1 and 2min showed a high EE of the dye (>90%). The W/O/W emulsion prepared by sonication for 3min showed an EE of<90%, but this EE was improved by adding 4αGTase-treated starch to the internal water phase. 4αGTase-treated starch was added to the internal water phase of W/O/W emulsions prepared with a low concentration of PGPR, and the PGPR concentration required to maintain an EE>90% was reduced. W/O/W emulsions containing 4αGTase-treated starch also showed better stability against heating and shearing stresses. These results indicated that 4αGTase-treated starch could be used in the preparation of W/O/W emulsions, which would allow the formulation of W/O/W emulsions with a reduced surfactant concentration.
authors.affiliation: Center for Agricultural Biomaterials and Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Saehun
authors.initials: S
authors.lastname: Mun
authors.affiliation: Molecular Imaging and Therapy Branch, National Cancer Center, Goyang-si 410-769, Republic of Korea.
authors.forename: Yongdoo
authors.initials: Y
authors.lastname: Choi
authors.affiliation: Department of Food and Biotechnology, Food and Bio-Industrial Research Center, Hankyong National University, Ansung, Gyeonggi 456-749, Republic of Korea.
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Shim
authors.affiliation: Department of Biology, University of Incheon, Incheon 402-749, Republic of Korea.
authors.forename: Kwan-Hwa
authors.initials: KH
authors.lastname: Park
authors.affiliation: Center for Agricultural Biomaterials and Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, Republic of Korea. Electronic address: yongro@snu.ac.kr.
authors.forename: Yong-Ro
authors.initials: YR
authors.lastname: Kim
keywords: 4-α-Glucanotransferase; PGPR; Stabilization; W/O/W emulsion