title: Histopathological aspects of liver under variable food restriction: has the intense one-week food restriction a protective effect on non-alcoholic-fatty-liver-disease (NAFLD) development?
abstract: Non-alcoholic-fatty-liver-disease (NAFLD) is a clinicopathologic entity characterized by a variety of hepatic injury patterns without significant alcohol use. It has a close association with obesity, so treatment includes weight loss, control of insulin sensitivity, interventions directed at inflammation and fibrosis. There is a certain relationship between the grade and duration of food restriction and hepatic function. The objective of this work was to describe the relationship between biochemistry, autoantibodies, insulin-like growth factor I (IGF-I), insulin-like growth factor binding protein 3 (IGFBP-3), and liver morphology in experimental rabbit groups with food restriction as compared to controls with ad libitum food (ADL) income. The experiment was performed on a total of 24 rabbits of a weaning age of 25-81 days. The first group (R1) was restricted between 32 and 39 days of age to 50 g of food per rabbit a day. The second group (R2) was also restricted between 32 and 39 days, but the rabbits received 65 g of food per rabbit a day. At the end of the experiment, the blood and liver samples were collected at necropsy. NAFLD has developed in all three groups. There was any autoantibody positivity in all three groups. IGF-I is moderately higher in R1 and R2 group, as compared to the control group (P > 0.05). IGFBP-3 is without statistical significance in all three groups. Alkaline phosphatase (ALP) is the only liver biochemical parameter that has significantly increased following food restriction (P > 0.039). Single one-week restriction has any protective effect on NAFLD development.
authors.affiliation: Laboratory of Veterinary Histopathology in Komarno, Slovak Republic. Electronic address: pmakovicky@email.cz.
authors.forename: Peter
authors.initials: P
authors.lastname: Makovicky
authors.affiliation: Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Tumova
authors.affiliation: Physiology of Nutrition and Quality of Animal Product, Institute of Animal Science in Prague - Uhrineves, Czech Republic.
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Volek
authors.affiliation: Department of Biology, Pedagogical Faculty, Selye Janos University in Komarno, Slovak Republic.
authors.forename: Pavol
authors.initials: P
authors.lastname: Makovicky
authors.affiliation: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic in Prague, Czech Republic.
authors.forename: Ludmila
authors.initials: L
authors.lastname: Vodickova
authors.affiliation: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic in Prague, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Slyskova
authors.affiliation: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic in Prague, Czech Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Svoboda
authors.affiliation: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic in Prague, Czech Republic.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Rejhova
authors.affiliation: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic in Prague, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Vodicka
authors.affiliation: Department of Immunology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Samasca
authors.affiliation: Student of Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Kralova
authors.affiliation: Department of Biology, Pedagogical Faculty, Selye Janos University in Komarno, Slovak Republic.
authors.forename: Melinda
authors.initials: M
authors.lastname: Nagy
authors.affiliation: Department of Biology, University of Presov in Presov, Slovak Republic.
authors.forename: Marta
authors.initials: M
authors.lastname: Mydlarova-Blascakova
authors.affiliation: Department of Biology, University of Presov in Presov, Slovak Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Poracova
mesh_terms: D000367:Age Factors; D000818:Animals; D001323:Autoantibodies / Q000097:blood; D015415:Biomarkers / Q000097:blood; D031204:Caloric Restriction; D019610:Cytoprotection; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008099:Liver / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D011817:Rabbits; D013997:Time Factors; D015430:Weight Gain
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D018972:Insulin-Like Growth Factor Binding Protein 3; D007334:Insulin-Like Growth Factor I
keywords: Fasting; Liver; Mitochondrion; NAFLD; NASH; Starvation