title: [Inhibition of Th2 reaction by thymic stromal lymphopoietin blockade in vitro].
abstract: OBJECTIVE
  To explore the effect of thymic stromal lymphopoietin (TSLP) on transformation of dendritic cell (DC) and T cell in vitro.
  
  
  METHODS
  Mouse-derived immature dendritic cells and T lymphocytes were co-cultured in vitro, which were divided into 4 groups (TSLP stimulation group, TSLP stimulation and its receptor blocking group, ovalbumin stimulation group and ovalbumin stimulation and TSLP receptor blocking group). IL-4, IL-8 and IFN-β in cell culture supernatant were detected after 2 days by ELISA. SPSS 13.0 software was used to analyze the data.
  
  
  RESULTS
  IL-4 levels of TSLP receptor blocking groups [(48.84 ± 1.56) pg/ml, (52.53 ± 2.36) pg/ml]were significantly lower than those of corresponding TSLP stimulation group and ovalbumin stimulation group [(72.55 ± 7.76) pg/ml, (80.47 ± 21.93) pg/ml;t = 5.994, P < 0.05;t = 2.534, P < 0.05]. However, there were not significant differences of IL-8 and IFN-β expression between corresponding two groups of whether or not TSLP receptor blocking (all P > 0.05).
  
  
  CONCLUSIONS
  TSLP receptor blockade in vitro can inhibit T lymphocyte transformation to Th2, which may provide a new therapeutic strategy for clinical Th2 dominant diseases such as allergic rhinitis and asthma.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Liting
authors.initials: L
authors.lastname: Wen
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Qiao
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Mi
authors.forename: Ye
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Email: qiujh@fmmu.edu.cn.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Qiu
mesh_terms: D000818:Animals; D054338:Cell Transdifferentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000037:antagonists & inhibitors*; D003713:Dendritic Cells / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D018418:Th2 Cells / Q000166:cytology*; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D016207:Cytokines; D000094632:Thymic Stromal Lymphopoietin; C000721967:TSLP protein, mouse