title: Highly sensitive graphene-Pt nanocomposites amperometric biosensor and its application in living cell H2O2 detection.
abstract: A sensitive hydrogen peroxide (H2O2) sensor was constructed based on graphene-Pt (RGO-Pt) nanocomposites and used to measure the release of H2O2 from living cells. The graphene and Pt nanoparticles (Pt NPs) were modified on glassy carbon electrode (GCE) by the physical adsorption and electrodeposition of K2PtCl6 solution, respectively. Through characterization by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS), it was observed that the electrodeposited Pt NPs were densely covered and well distributed on the entire graphene surface. Electrochemical study demonstrates that the RGO-Pt nanocomposites modified glassy carbon electrode exhibited a high peak current and low overpotential toward the reduction of H2O2. The relevant detection limit of H2O2 is ∼0.2 μM with a wide linear range from 0.5 μM to 3.475 mM, displaying a much higher sensitivity (459 ± 3 mA M(-1) cm(-2), n = 5) than that of Pt nanoparticles or graphene modified electrode. This novel biosensor can measure the H2O2 release from living cells because of its low detection limit, wide linear range, and higher sensitivity.
authors.affiliation: State Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University , Nanjing 210096, P. R. China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Bai
authors.forename: Xuemei
authors.initials: X
authors.lastname: Wang
authors.forename: Kwok-Keung
authors.initials: KK
authors.lastname: Shiu
authors.forename: Yanliang
authors.initials: Y
authors.lastname: Zhu
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry / Q000494:pharmacology; D015374:Biosensing Techniques; D004566:Electrodes; D004595:Electroplating; D006108:Graphite / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D057230:Limit of Detection; D053761:Nanocomposites / Q000737:chemistry*; D010084:Oxidation-Reduction / Q000187:drug effects; D010087:Oxides; D016716:PC12 Cells; D010984:Platinum / Q000737:chemistry*; D051381:Rats
chemical_list: D010087:Oxides; D010984:Platinum; D006108:Graphite; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid