title: Whole-mount in situ hybridization using DIG-labeled probes in planarian.
abstract: In recent years freshwater flatworms (planarian) have become a powerful model for studies of regeneration and stem cell biology. Whole-mount in situ hybridization (WISH) and fluorescent in situ hybridization (FISH) are key and most commonly used techniques to determine and visualize gene expression patterns in planaria. Here, we present the established version of whole-mount in situ hybridization (WISH) and whole-mount fluorescence in situ hybridization (WFISH) protocol optimized over the last years by several labs from the rapidly growing planaria field and give an overview of recently introduced modifications which can be critical in the study of low abundant transcripts.
authors.affiliation: Laboratory for Systems Biology of Gene Regulatory Elements, Berlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular Medicine, Robert-Rössle-Strasse 10, 13125, Berlin, Germany, agnieszka.rybak@mdc-berlin.de.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Rybak-Wolf
authors.forename: Jordi
authors.initials: J
authors.lastname: Solana
mesh_terms: D000818:Animals; D001483:Base Sequence; D004076:Digoxigenin / Q000032:analysis*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D010932:Planarians / Q000235:genetics*; D015347:RNA Probes / Q000032:analysis* / Q000235:genetics; D018510:RNA, Helminth / Q000032:analysis* / Q000235:genetics
chemical_list: D015347:RNA Probes; D018510:RNA, Helminth; D004076:Digoxigenin