title: Multi-laboratory evaluation of the rapid genoserotyping array (SGSA) for the identification of Salmonella serovars.
abstract: Salmonella serotyping is an essential first step for identification of isolates associated with disease outbreaks. The Salmonella genoserotyping array (SGSA) is a microarray-based alternative to standard serotyping designed to rapidly identify 57 of the most commonly reported serovars through detection of the genes encoding surface O and H antigens and reporting the corresponding serovar in accordance with the existing White-Kaufmann-Le Minor serotyping scheme. In this study, we evaluated the SGSA at 4 laboratories in 3 countries by testing 1874 isolates from human and non-human sources. The SGSA correctly identified 96.7% of isolates from the target 57 serovars. For the prevalent and clinically important Salmonella serovars Enteritidis and Typhimurium, test specificity and sensitivity were greater than 98% and 99%, respectively. Due to its high-throughput nature, the SGSA is a rapid and cost-effective alternative to standard serotyping for identifying the most prevalent serovars of Salmonella.
authors.affiliation: Laboratory for Foodborne Zoonoses, Public Health Agency of Canada (PHAC), Guelph, Ontario, Canada N1G 3W4. Electronic address: catherine.yoshida@phac-aspc.gc.ca.
authors.forename: Catherine
authors.initials: C
authors.lastname: Yoshida
authors.affiliation: Laboratory for Foodborne Zoonoses, Public Health Agency of Canada (PHAC), Guelph, Ontario, Canada N1G 3W4.
authors.forename: Erika J
authors.initials: EJ
authors.lastname: Lingohr
authors.affiliation: Austrian Institute of Technology (AIT), 3430 Tulln an der Donau, Austria.
authors.forename: Friederike
authors.initials: F
authors.lastname: Trognitz
authors.affiliation: Animal Health and Veterinary Laboratories Agency (AHVLA), New Haw, Addlestone, Surrey, United Kingdom KT15 3NB.
authors.forename: Nikki
authors.initials: N
authors.lastname: MacLaren
authors.affiliation: Laboratory for Foodborne Zoonoses, Public Health Agency of Canada (PHAC), Guelph, Ontario, Canada N1G 3W4.
authors.forename: Andrea
authors.initials: A
authors.lastname: Rosano
authors.affiliation: Laboratory for Foodborne Zoonoses, Public Health Agency of Canada (PHAC), Guelph, Ontario, Canada N1G 3W4.
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Murphy
authors.affiliation: Laboratory for Foodborne Zoonoses, Public Health Agency of Canada (PHAC), Guelph, Ontario, Canada N1G 3W4.
authors.forename: Andre
authors.initials: A
authors.lastname: Villegas
authors.affiliation: Austrian Institute of Technology (AIT), 3430 Tulln an der Donau, Austria.
authors.forename: Marlies
authors.initials: M
authors.lastname: Polt
authors.affiliation: Laboratory for Foodborne Zoonoses, Public Health Agency of Canada (PHAC), Guelph, Ontario, Canada N1G 3W4.
authors.forename: Kristyn
authors.initials: K
authors.lastname: Franklin
authors.affiliation: Austrian Institute of Technology (AIT), 3430 Tulln an der Donau, Austria.
authors.forename: Tanja
authors.initials: T
authors.lastname: Kostic
authors.affiliation: Laboratory for Foodborne Zoonoses, Public Health Agency of Canada (PHAC), Guelph, Ontario, Canada N1G 3W4.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Kropinski
authors.affiliation: Animal Health and Veterinary Laboratories Agency (AHVLA), New Haw, Addlestone, Surrey, United Kingdom KT15 3NB.
authors.forename: Roderick M
authors.initials: RM
authors.lastname: Card
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D046228:Microarray Analysis / Q000379:methods; D019081:O Antigens / Q000235:genetics; D012475:Salmonella / Q000145:classification* / Q000235:genetics*; D012480:Salmonella Infections / Q000175:diagnosis / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000175:diagnosis / Q000382:microbiology; D012680:Sensitivity and Specificity; D065288:Serogroup; D012703:Serotyping / Q000379:methods*
chemical_list: D000942:Antigens, Bacterial; C022863:H antigen; D019081:O Antigens
keywords: Evaluation; H-antigen; Microarray; O-antigen; Salmonella; Serotyping