title: Efficient gene editing in adult mouse livers via adenoviral delivery of CRISPR/Cas9.
abstract: We developed an adenovirus-based CRISPR/Cas9 system for gene editing in vivo. In the liver, we demonstrated that the system could reach the level of tissue-specific gene knockout, resulting in phenotypic changes. Given the wide spectrum of cell types susceptible to adenoviral infection, and the fact that adenoviral genome rarely integrates into its host cell genome, we believe the adenovirus-based CRISPR/Cas9 system will find applications in a variety of experimental settings.
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China; The College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100142, China.
authors.forename: Ranran
authors.initials: R
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China; The Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai 200241, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China.
authors.forename: Peili
authors.initials: P
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China; Department of Gastroenterology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hanzhou, Zhejiang Province 310011, China.
authors.forename: Jiewei
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China.
authors.forename: Lichun
authors.initials: L
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China.
authors.forename: Di
authors.initials: D
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China.
authors.forename: Li
authors.initials: L
authors.lastname: Tang
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Texas Health Sciences Center, Houston, TX 77030, United States.
authors.forename: Jianping
authors.initials: J
authors.lastname: Jin
authors.affiliation: Model Animal Research Center, Nanjing University, Nanjing, Jiangsu Province 210061, China.
authors.forename: Xingxu
authors.initials: X
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China; National Center for Protein Sciences Beijing, Life Sciences Park, Beijing 102206, China.
authors.forename: Fuchu
authors.initials: F
authors.lastname: He
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China; National Center for Protein Sciences Beijing, Life Sciences Park, Beijing 102206, China; Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, United States. Electronic address: pzhang@bcm.edu.
authors.forename: Pumin
authors.initials: P
authors.lastname: Zhang
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice
keywords: Adenoviruses; CRISPR/Cas9; Gene editing; Liver