title: Effect of post recruitment maneuver ventilation by different tidal volume on lung vascular endothelial diastole function in rats with acute lung injury.
abstract: BACKGROUND
  This study aimed to observe the effect of recruitment maneuver (RM) and post-RM ventilation at different tidal volume on lung vascular diastole endothelial function in rats with acute lung injury (ALI).
  
  
  METHODS
  A ALI rat model was produced by intravenous infusion of lipopolysaccharide (6 mg/ kg). Twenty-five rats were randomly divided into five groups: control group (n=5), ALI group (n=5), low tidal volume group (LV group, VT 6 mL/kg, n=5), sustained inflation (SI) with low tidal volume group (SI+LV group, VT 6 mL/kg, n=5), and SI with moderate tidal volume group (SI+MV group, VT 12 mL/ kg, n=5). RM was performed with SI, airway pressure 30 cmH2O for 30 seconds, and positive end-expiratory pressure (PEEP) was set to 5 cmH2O. Lung tissue was taken after 5 hours of mechanical ventilation. Mean arterial blood pressure (MAP) was monitored during the experiment. Endothelin-1 (ET-1), endothelial nitricoxide synthase (eNOS), Ach-induced endothelium-dependent relaxation response of isolated pulmonary artery rings were determined at 5 hours.
  
  
  RESULTS
  LPS increased ET-1 level, decreased the expression of eNOS in lung tissue, impaired the Ach-induced endothelium-dependent relaxation response in the pulmonary artery, without obvious effect on systemic hemodynamics. SI+LV significantly reduced LPS-induced elevation of ET-1 level, increased the expression of eNOS, significantly improved endothelial dysfunction, and improved the dysfunction of endothelium-dependent relaxation in the pulmonary artery.
  
  
  CONCLUSIONS
  RM with a high or low tidal volume ventilation could improve the lung vascular endothelial function of rats with acute lung injury, and RM with low tidal volume ventilation could lower significantly the injury of lung vascular endothelial diastole function in rats with acute lung injury.
authors.affiliation: Department of Critical Care Medicine, Jintan Hospital Affiliated to Jiangsu University, Jintan 213200, China; ICU, Zhongda Hospital, South-East University, Nanjing 210009, China (Pan C, Liu L, Jin L, Yang Y, Qiu HB).
authors.forename: Jian-Qiang
authors.initials: JQ
authors.lastname: Wang
authors.affiliation: Department of Critical Care Medicine, Jintan Hospital Affiliated to Jiangsu University, Jintan 213200, China; ICU, Zhongda Hospital, South-East University, Nanjing 210009, China (Pan C, Liu L, Jin L, Yang Y, Qiu HB).
authors.forename: Chun
authors.initials: C
authors.lastname: Pan
authors.affiliation: Department of Critical Care Medicine, Jintan Hospital Affiliated to Jiangsu University, Jintan 213200, China; ICU, Zhongda Hospital, South-East University, Nanjing 210009, China (Pan C, Liu L, Jin L, Yang Y, Qiu HB).
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Critical Care Medicine, Jintan Hospital Affiliated to Jiangsu University, Jintan 213200, China; ICU, Zhongda Hospital, South-East University, Nanjing 210009, China (Pan C, Liu L, Jin L, Yang Y, Qiu HB).
authors.forename: Liang
authors.initials: L
authors.lastname: Jin
authors.affiliation: Department of Critical Care Medicine, Jintan Hospital Affiliated to Jiangsu University, Jintan 213200, China; ICU, Zhongda Hospital, South-East University, Nanjing 210009, China (Pan C, Liu L, Jin L, Yang Y, Qiu HB).
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Critical Care Medicine, Jintan Hospital Affiliated to Jiangsu University, Jintan 213200, China; ICU, Zhongda Hospital, South-East University, Nanjing 210009, China (Pan C, Liu L, Jin L, Yang Y, Qiu HB).
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Qiu
keywords: Acute lung injury; Low tidal volume; Lung vascular endothelium; Pulmonary artery diastole; Recruitment maneuver