title: Direct interaction of actin filaments with F-BAR protein pacsin2.
abstract: Two mechanisms have emerged as major regulators of membrane shape: BAR domain-containing proteins, which induce invaginations and protrusions, and nuclear promoting factors, which cause generation of branched actin filaments that exert mechanical forces on membranes. While a large body of information exists on interactions of BAR proteins with membranes and regulatory proteins of the cytoskeleton, little is known about connections between these two processes. Here, we show that the F-BAR domain protein pacsin2 is able to associate with actin filaments using the same concave surface employed to bind to membranes, while some other tested N-BAR and F-BAR proteins (endophilin, CIP4 and FCHO2) do not associate with actin. This finding reveals a new level of complexity in membrane remodeling processes.
authors.affiliation: Department of Structural and Computational Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
authors.forename: Julius
authors.initials: J
authors.lastname: Kostan
authors.affiliation: Department of Structural and Computational Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria Department of Medical Biochemistry, Medical University of Vienna, Vienna, Austria.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Salzer
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Virginia Medical Center, Charlottesville, VA, USA.
authors.forename: Albina
authors.initials: A
authors.lastname: Orlova
authors.affiliation: Department of Structural and Computational Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
authors.forename: Imre
authors.initials: I
authors.lastname: Törö
authors.affiliation: Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Hodnik
authors.affiliation: Program in Cell and Molecular Biology, Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Senju
authors.affiliation: Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK.
authors.forename: Juan
authors.initials: J
authors.lastname: Zou
authors.affiliation: Department of Structural and Computational Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
authors.forename: Claudia
authors.initials: C
authors.lastname: Schreiner
authors.affiliation: Department of Structural and Computational Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
authors.forename: Julia
authors.initials: J
authors.lastname: Steiner
authors.affiliation: Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Finland.
authors.forename: Jari
authors.initials: J
authors.lastname: Meriläinen
authors.affiliation: Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Finland.
authors.forename: Marko
authors.initials: M
authors.lastname: Nikki
authors.affiliation: Institute of Biomedicine/Anatomy, University of Helsinki, Helsinki, Finland.
authors.forename: Ismo
authors.initials: I
authors.lastname: Virtanen
authors.affiliation: Department of Structural and Computational Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria Department of Chemistry, University of Pavia, Pavia, Italy.
authors.forename: Oliviero
authors.initials: O
authors.lastname: Carugo
authors.affiliation: Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Finland Department of Biotechnology, Technological University of Berlin, Berlin, Germany.
authors.forename: Juri
authors.initials: J
authors.lastname: Rappsilber
authors.affiliation: Program in Cell and Molecular Biology, Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
authors.forename: Pekka
authors.initials: P
authors.lastname: Lappalainen
authors.affiliation: Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Finland.
authors.forename: Veli-Pekka
authors.initials: VP
authors.lastname: Lehto
authors.affiliation: Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia National Institute of Chemistry, Ljubljana, Slovenia EN-FIST Centre of Excellence, Ljubljana, Slovena.
authors.forename: Gregor
authors.initials: G
authors.lastname: Anderluh
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Virginia Medical Center, Charlottesville, VA, USA.
authors.forename: Edward H
authors.initials: EH
authors.lastname: Egelman
authors.affiliation: Department of Structural and Computational Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria Department of Biochemistry, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, Slovenia kristina.djinovic@univie.ac.at.
authors.forename: Kristina
authors.initials: K
authors.lastname: Djinović-Carugo
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry / Q000378:metabolism*; D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D062105:Molecular Docking Simulation; D008969:Molecular Sequence Data; D011485:Protein Binding
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D008869:Microtubule-Associated Proteins
keywords: F‐BAR protein pacsin2; F‐actin binding; cryo‐electron microscopy; membrane sculpting