title: Astaxanthin is responsible for antiglycoxidative properties of microalga Chlorella zofingiensis.
abstract: The antiglycoxidative properties of microalga Chlorella zofingiensis were investigated for the first time in this study. Algal extracts containing different contents of astaxanthin were prepared. Through the comparison, it was shown that the extract rich in astaxanthin exhibited higher antioxidant abilities as well as stronger antiglycative capacities, including the inhibition of advanced glycation endproducts (AGEs) formation, glucose autoxidation as well as glycation-induced protein oxidation. The extract was further fractionated using TLC. Among all fractions obtained, the fraction of astaxanthin in diester form was found to contain the strongest inhibitory effects on the glycation cascade. Its tentative structure was subsequently identified by LC-MS analysis. These results clearly ascertained the antiglycoxidative properties of astaxanthin derived from C. zofingiensis and supported the possibility of using natural antioxidants as glycation inhibitors. The microalga C. zofingiensis, therefore, might be the beneficial food and preventive agent choice for diabetic patients.
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Sun
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zeng
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China.
authors.forename: Jieqiong
authors.initials: J
authors.lastname: Huangfu
authors.affiliation: Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China. Electronic address: mfwang@hkusua.hku.hk.
authors.forename: Mingfu
authors.initials: M
authors.lastname: Wang
authors.affiliation: School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China. Electronic address: sfchen@hkusua.hku.hk.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
keywords: Astaxanthin; Chlorella zofingiensis; Diabetic complications; Glycoxidation