title: Monolithic molecularly imprinted polymeric capillary columns for isolation of aflatoxins.
abstract: Monolithic molecularly imprinted polymers extraction columns have been prepared in fused-silica capillaries by UV or thermal polymerization in a two-step process. First, a poly-(trimethylolpropane trimethacrylate) (polyTRIM) core monolith was synthesized either by UV or thermal polymerization. Then it was grafted with the mixture of methacrylic acid (MAA) as a functional monomer, ethylene dimethacrylate (EDMA) as a cross-linking agent, 5,7-dimethoxycoumarin (DMC) as an aflatoxin-mimicking template, toluene as a porogen solvent and 2,2-azobis-(2-methylpropionitrile) (AIBN) as an initiator of the polymerization reaction. Different thermal condition of the photografting and different concentrations of the grafting mixture were tested during polymerization. The extraction capillary columns were evaluated in the terms of their hydrodynamic and chromatographic properties. Retention coefficients for aflatoxin B1 and DMC were used for assessment of the selectivity and imprinting factor. The obtained results indicate that the temperature of photografting and concentration of the grafting mixture are key parameters that determine the quality of the prepared MIPs. From the MIP columns characterized by the highest permeability the column of the highest imprinting factor was applied for isolation of aflatoxins B1, B2, G1 and G2 from the model aqueous sample followed by on-line chromatographic separation. The process was performed using a micro-MISPE-microLC-LIF system of a novel design, which allowed for detection of the eluates from the sample preparation part as well as from the chromatographic separation.
authors.affiliation: Department of Environmental Chemistry and Bioanalytics, Nicolaus Copernicus University, Faculty of Chemistry Gagarina 7, PL-87-100 Toruń, Poland. Electronic address: michu@chem.umk.pl.
authors.forename: Michał
authors.initials: M
authors.lastname: Szumski
authors.affiliation: Department of Environmental Chemistry and Bioanalytics, Nicolaus Copernicus University, Faculty of Chemistry Gagarina 7, PL-87-100 Toruń, Poland.
authors.forename: Damian
authors.initials: D
authors.lastname: Grzywiński
authors.affiliation: Department of Biology and Environmental Chemistry, Institute of Engineering and Environmental Protection, University of Bielsko-Biała, Faculty of Materials and Environmental Sciences, Willowa 2, PL-43-309 Bielsko-Biała, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Prus
authors.affiliation: Department of Environmental Chemistry and Bioanalytics, Nicolaus Copernicus University, Faculty of Chemistry Gagarina 7, PL-87-100 Toruń, Poland.
authors.forename: Bogusław
authors.initials: B
authors.lastname: Buszewski
mesh_terms: D000348:Aflatoxins / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D003374:Coumarins / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry*; D054802:Molecular Imprinting; D010539:Permeability; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D012822:Silicon Dioxide; D014867:Water / Q000737:chemistry
chemical_list: D000348:Aflatoxins; D003374:Coumarins; D003432:Cross-Linking Reagents; D008689:Methacrylates; D011108:Polymers; D014867:Water; C008384:methacrylic acid; D012822:Silicon Dioxide; C004919:ethylene dimethacrylate; C037446:5,7-dimethoxycoumarin; C028643:trimethylolpropane trimethacrylate
keywords: Aflatoxins; Capillary liquid chromatography; Molecularly imprinted polymers; Monolithic column; Photopolymerization