title: Using detection dogs and genetic analyses of scat to expand knowledge and assist felid conservation in Misiones, Argentina.
abstract: Many carnivores require large ranges to meet their ecological and energetic needs; however, anthropogenic changes threaten species and their habitats. Camera traps have been used to effectively collect data on carnivores in a variety of habitat types; however, a single survey effort is typically limited to species that have similar body size, habitat use and movement patterns, and individual identification of animals is not always possible. We evaluated whether scat detection dogs could effectively survey for 4 wide-ranging felids that vary in these characteristics: jaguars (Panthera onca), pumas (Puma concolor), ocelots (Leopardus pardalis) and oncillas (Leopardus tigrinus). From June to October 2009 and May to August 2011, a detection dog-handler team detected 588 scats, from which 176 unique genotypes were detected. We assigned sex to 84.7% of the genotyped scats and identified 55 individuals multiple times. The effectiveness of these noninvasive techniques (detection dogs and genetic analyses of scat) not only opens the door for additional studies in areas that were previously difficult or impossible with standard survey techniques, but also provides conservationists with a set of tools that overcome some of the limitations associated with the use of camera traps alone.
authors.affiliation: Department of Biology, University of Missouri, St. Louis, USA; WildCare Institute at the Saint Louis Zoo, St. Louis, USA.
authors.forename: Karen E
authors.initials: KE
authors.lastname: DeMatteo
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Rinas
authors.forename: Carina F
authors.initials: CF
authors.lastname: Argüelles
authors.forename: Bernardo E
authors.initials: BE
authors.lastname: Holman
authors.forename: Mario S
authors.initials: MS
authors.lastname: Di Bitetti
authors.forename: Barbara
authors.initials: B
authors.lastname: Davenport
authors.forename: Patricia G
authors.initials: PG
authors.lastname: Parker
authors.forename: Lori S
authors.initials: LS
authors.lastname: Eggert
mesh_terms: D000818:Animals; D001118:Argentina; D003247:Conservation of Natural Resources / Q000379:methods*; D004272:DNA, Mitochondrial / Q000235:genetics; D004285:Dogs; D005243:Feces; D045989:Felidae / Q000235:genetics*; D005260:Female; D005838:Genotype; D008297:Male; D018895:Microsatellite Repeats; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial
keywords: conservation; detection dogs; felids; genetics; noninvasive techniques