title: Bio-nanogate controlled enzymatic reaction for virus sensing.
abstract: The objective of this study was to develop an aptamer-based bifunctional bio-nanogate, which could selectively respond to target molecules, and control enzymatic reaction for electrochemical measurements. It was successfully applied for sensitive, selective, rapid, quantitative, and label-free detection of avian influenza viruses (AIV) H5N1. A nanoporous gold film with pore size of ~20 nm was prepared by a metallic corrosion method, and the purity was checked by energy-dispersive X-ray spectroscopy (EDS) study. To improve the performance of the bio-nanogate biosensor, its main analytical parameters were studied and optimized. We demonstrated that the developed bio-nanogate was capable of controlling enzymatic reaction for AIV H5N1 sensing within 1h with a detection limit of 2(-9)HAU (hemagglutination units). The enzymatic reaction was able to cause significant current change due to the presence of target AIV. A linear relationship was found in the virus titer range of 2(-10)-2(2)HAU. No interference was observed from non-target AIV subtypes such as H1N1, H2N2, H4N8 and H7N2. The developed approach could be adopted for sensing of other viruses.
authors.affiliation: Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA.
authors.forename: Ronghui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310068, China.
authors.forename: Lizhou
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310068, China; Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA. Electronic address: yanbinli@uark.edu.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D001717:Birds; D006046:Gold / Q000737:chemistry; D006387:Hemagglutination, Viral; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000821:virology*; D057230:Limit of Detection
chemical_list: D006046:Gold
keywords: Aptamer; Avian influenza; Bio-nanogate; Enzymatic reaction; Virus sensing