title: Production and characterization of high efficiency bioflocculant isolated from Klebsiella sp. ZZ-3.
abstract: In this study, a new bioflocculant (ZZ-3) is isolated and evaluated. This novel flocculant was derived Klebsiella, which was identified by 16S rDNA sequencing as well as biochemical and physiological analyses. The composition of ZZ-3 was found to be 84.6% polysaccharides and 6.1% protein. More specifically, the amount (moles) of the polysaccharides rhamnose, mannose, and galactose were found to be 6.48, 2.47, and 1.74 greater than glucose, respectively. Results show ZZ-3 has a relatively high molecular weight (603-1820 kDa) and contains many functional groups (hydroxyl, amide, carboxyl, and methoxyl) that likely contribute to flocculation. The results of microscopic observation, zeta potential measurements, and ZZ-3 bioflocculant structure suggested that bridging was the main mechanism for flocculation with kaolin. Based on a high flocculation efficiency, thermal stability, pH tolerance and the ability to flocculate without additional cations, ZZ-3 shows potential for industrial application.
authors.affiliation: Environmental Microbiology and Ecology Research Center, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, PR China.
authors.forename: Ya-Jie
authors.initials: YJ
authors.lastname: Yin
authors.affiliation: Shanghai Information Center for Life Sciences, Shanghai Institute of Biological Sciences, Chinese Academy of Science, Shanghai 200031, PR China.
authors.forename: Zun-Ming
authors.initials: ZM
authors.lastname: Tian
authors.affiliation: Environmental Microbiology and Ecology Research Center, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.affiliation: Environmental Microbiology and Ecology Research Center, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Environmental Microbiology and Ecology Research Center, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, PR China; Key Laboratory of Reservoir Aquatic Environment, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, PR China. Electronic address: songliyan@cigit.ac.cn.
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Song
authors.affiliation: Department of Civil & Environmental Engineering, Wayne State University, Detroit, MI 48202, USA.
authors.forename: Shawn P
authors.initials: SP
authors.lastname: McElmurry
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D005427:Flocculation; D005690:Galactose / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D007218:Industrial Microbiology / Q000379:methods*; D007709:Klebsiella / Q000235:genetics / Q000378:metabolism*; D046911:Macromolecular Substances / Q000737:chemistry* / Q000378:metabolism*; D008358:Mannose / Q000032:analysis; D056951:Photoelectron Spectroscopy; D016133:Polymerase Chain Reaction; D011135:Polysaccharides, Bacterial / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012210:Rhamnose / Q000032:analysis; D013055:Spectrophotometry, Infrared
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D046911:Macromolecular Substances; D011135:Polysaccharides, Bacterial; D012336:RNA, Ribosomal, 16S; D008358:Mannose; D012210:Rhamnose; D005690:Galactose
keywords: Bioflocculant; Industrial; Klebsiella sp. ZZ-3; Thermal stability; pH tolerant