title: Induction of cancer-related microRNA expression profiling using excretory-secretory products of Clonorchis sinensis.
abstract: Clonorchis sinensis is a carcinogenic human liver fluke by which chronic infection is strongly associated with the development of cholangiocarcinoma. Although this cholangiocarcinoma is caused by both physical and chemical irritation from direct contact with adult worms and their excretory-secretory products (ESPs), the precise molecular events of the host-pathogen interactions remain to be elucidated. To better understand the effect of C. sinensis infection on cholangiocarcinogenesis, we profiled the kinetics of changes in cancer-related microRNAs (miRNAs) in human cholangiocarcinoma cells (HuCCT1) treated with C. sinensis ESPs for different periods. Using miRNA microarray chips containing 135 cancer-related miRNAs, we identified 16 miRNAs showing differentially altered expression following ESP exposure. Of these miRNAs, 13 were upregulated and 3 were downregulated in a time-dependent manner compared with untreated controls. Functional clustering of these dysregulated miRNAs revealed involvement in cell proliferation, inflammation, oncogene activation/suppression, migration/invasion/metastasis, and DNA methylation. In particular, decreased expression of let-7i, a tumor suppressor miRNA, was found to be associated with the ESP-induced upregulation of TLR4 mRNA and protein, which contribute to host immune responses against liver fluke infection. Further real-time quantitative PCR analysis using ESP-treated normal cholangiocytes (H69) revealed that the expressions of nine miRNAs (miR-16-2, miR-93, miR-95, miR-153, miR-195, miR-199-3P, let7a, let7i, and miR-124a) were similarly regulated, indicating that the cell proliferation and inhibition of tumor suppression mediated by these miRNAs is common to both cancerous and non-cancerous cells. These findings constitute further our understanding of the multiple cholangiocarcinogenic pathways triggered by liver fluke infection.
authors.affiliation: Asan Institute for Life Sciences, University of Ulsan College of Medicine Asan Medical Center, 388-1 Punganp-2 dong, Songpa-gu, Seoul, 138-736, Republic of Korea, jhpak@amc.seoul.kr.
authors.forename: Jhang Ho
authors.initials: JH
authors.lastname: Pak
authors.forename: In Ki
authors.initials: IK
authors.lastname: Kim
authors.forename: Seon Min
authors.initials: SM
authors.lastname: Kim
authors.forename: Sejung
authors.initials: S
authors.lastname: Maeng
authors.forename: Kyoung Ju
authors.initials: KJ
authors.lastname: Song
authors.forename: Byoung-Kuk
authors.initials: BK
authors.lastname: Na
authors.forename: Tong-Soo
authors.initials: TS
authors.lastname: Kim
mesh_terms: D000818:Animals; D001650:Bile Duct Neoplasms / Q000469:parasitology*; D001653:Bile Ducts, Intrahepatic; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018281:Cholangiocarcinoma / Q000469:parasitology*; D003003:Clonorchiasis / Q000150:complications*; D003004:Clonorchis sinensis / Q000235:genetics* / Q000472:pathogenicity; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D046228:Microarray Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015533:Transcriptional Activation; D015854:Up-Regulation
chemical_list: D015801:Helminth Proteins; D035683:MicroRNAs; D012333:RNA, Messenger