title: Quantitative determination of euphol in rat  plasma by LC-MS/MS and its application to  a pharmacokinetic study.
abstract: Euphol is a potential pharmacologically active ingredient isolated from Euphorbia kansui. A simple, rapid, and sensitive method to determine euphol in rat plasma was developed based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the first time. The analyte and internal standard (IS), oleanic acid, were extracted from plasma with methanol and chromatographied on a C18 short column eluted with a mobile phase of methanol–water–formic acid (95:5:0.1, v/v/v). Detection was performed by positive ion atmospheric pressure chemical ionization in selective reaction monitoring mode. This method monitored the transitions m/z 409.0 →109.2 and m/z 439.4 → 203.2 for euphol and IS, respectively. The assay was linear over the concentration range 27–9000 ng/mL, with a limit of quantitation of 27 ng/mL. The accuracy was between –7.04 and 4.11%, and the precision was <10.83%. This LC-MS/MS method was successfully applied to investigate the pharmacokinetic study of euphol in rats after intravenous (6 mg/kg) and oral (48 mg/kg) administration. Results showed that the absolute bioavailability of euphol was approximately 46.01%.
authors.forename: Xu
authors.initials: X
authors.lastname: Xie
authors.forename: Yongning
authors.initials: Y
authors.lastname: Li
authors.forename: Dongna
authors.initials: D
authors.lastname: Gao
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Ren
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D007810:Lanosterol / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D016014:Linear Models; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007810:Lanosterol; C062557:euphol