title: Canthaxanthin biosynthesis by Dietzia natronolimnaea HS-1: effects of inoculation and aeration rate.
abstract: The interest in production of natural colorants by microbial fermentation has been currently increased. The effects of D-glucose concentration (3.18-36.82 g/L), inoculum size (12.5 × 10(9)-49.5 × 10(9) cfu cells/mL) and air-flow rate (1.95-12.05 L/L min) on the biomass, total carotenoid and canthaxanthin (CTX) accumulation of Dietzia natronolimnaea HS-1 in a batch bioreactor was scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD). Second-order polynomial models with high R (2) values ranging from 0.978 to 0.990 were developed for the studied responses using multiple linear regression analysis. The models showed the maximum cumulative amounts of biomass (7.85 g/L), total carotenoid (5.48 mg/L) and CTX (4.99 mg/L) could be achieved at 23.38 g/L of D-glucose, 31.2 × 10(9) cfu cells/mL of inoculation intensity and air-flow rate of 7.85 L/L min. The predicted values for optimum conditions were in good agreement with experimental data.
authors.affiliation: Bioprocess Engineering Laboratory Department of Food Science & Engineering Faculty of Agricultural Engineering and Technology University of Tehran Karaj Iran Bioprocess Engineering Laboratory, Department of Food Science & Engineering, Faculty of Agricultural Engineering and Technology, University of Tehran, Karaj, Iran. ; Iranian Center of Excellence for Application of Modern Technologies for Producing Functional Foods and Drinks University of Tehran Karaj Iran Iranian Center of Excellence for Application of Modern Technologies for Producing Functional Foods and Drinks, University of Tehran, Karaj, Iran.
authors.forename: Forouzan
authors.initials: F
authors.lastname: Rostami
authors.affiliation: Bioprocess Engineering Laboratory Department of Food Science & Engineering Faculty of Agricultural Engineering and Technology University of Tehran Karaj Iran Bioprocess Engineering Laboratory, Department of Food Science & Engineering, Faculty of Agricultural Engineering and Technology, University of Tehran, Karaj, Iran. ; Iranian Center of Excellence for Application of Modern Technologies for Producing Functional Foods and Drinks University of Tehran Karaj Iran Iranian Center of Excellence for Application of Modern Technologies for Producing Functional Foods and Drinks, University of Tehran, Karaj, Iran.
authors.forename: Seyed Hadi
authors.initials: SH
authors.lastname: Razavi
authors.affiliation: Iranian Center of Excellence for Application of Modern Technologies for Producing Functional Foods and Drinks University of Tehran Karaj Iran Iranian Center of Excellence for Application of Modern Technologies for Producing Functional Foods and Drinks, University of Tehran, Karaj, Iran.
authors.forename: Abbas Akhavan
authors.initials: AA
authors.lastname: Sepahi
authors.affiliation: Bioprocess Engineering Laboratory Department of Food Science & Engineering Faculty of Agricultural Engineering and Technology University of Tehran Karaj Iran Bioprocess Engineering Laboratory, Department of Food Science & Engineering, Faculty of Agricultural Engineering and Technology, University of Tehran, Karaj, Iran. ; Iranian Center of Excellence for Application of Modern Technologies for Producing Functional Foods and Drinks University of Tehran Karaj Iran Iranian Center of Excellence for Application of Modern Technologies for Producing Functional Foods and Drinks, University of Tehran, Karaj, Iran.
authors.forename: Seyed Mohammad Taghi
authors.initials: SM
authors.lastname: Gharibzahedi
mesh_terms: D039903:Actinobacteria / Q000254:growth & development* / Q000378:metabolism*; D000332:Aerobiosis; D000388:Air; D058491:Bacterial Load; D061249:Batch Cell Culture Techniques; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D016644:Canthaxanthin / Q000096:biosynthesis*; D005947:Glucose / Q000378:metabolism; D015233:Models, Statistical
chemical_list: D016644:Canthaxanthin; D005947:Glucose
keywords: Dietzia; batch bioreactor; microbial canthaxanthin; modeling; response surface methodology