title: [Use of adipose-derived stem cells in an experimental rotator cuff fracture animal model].
abstract: OBJECTIVE
  Rotator cuff repairs have shown a high level of re-ruptures. We hypothesized that the use of adipose-derived stem cells (ASC) could improve the biomechanical and histological properties of the repair.
  
  
  METHODS
  Controlled experimental study conducted on 44 BDIX rats with section and repair of the supraspinatus tendon and randomization to one of three groups: group A, no intervention (control); group B, local applications of a fibrin sealant; and group C, application of the fibrin sealant with 2 x 10(6) ASC. At 4 and 8 weeks a biomechanical and histological analysis was performed.
  
  
  RESULTS
  There were no differences in load-to-failure at 4 and 8 weeks between groups. The load-to-failure did increase between week 4 and week 8. Histologically the tendon-to bone union showed a disorganized fibrovascular tissue. Group C showed a different inflammatory pattern, with less presence of neutrophils and more presence of plasma cells.
  
  
  CONCLUSIONS
  The use of ASC does not improve the biomechanical or histological properties of the repair site. More studies are needed to improve techniques that enhance the healing site of the repair.
authors.affiliation: Servicio de Cirugía Ortopédica y Traumatología, IDIPAZ, Madrid, España. Electronic address: raulbarco@hotmail.com.
authors.forename: R
authors.initials: R
authors.lastname: Barco
authors.affiliation: Servicio de Cirugía Ortopédica y Traumatología, IDIPAZ, Madrid, España.
authors.forename: C
authors.initials: C
authors.lastname: Encinas
authors.affiliation: Servicio de Cirugía Ortopédica y Traumatología, IDIPAZ, Madrid, España.
authors.forename: M
authors.initials: M
authors.lastname: Valencia
authors.affiliation: Departamento de Medicina. Escuela Técnica Superior Ingeniaría Industrial, UNED, Madrid, España.
authors.forename: M T
authors.initials: MT
authors.lastname: Carrascal
authors.affiliation: Unidad de Terapia Celular, Instituto de Investigación Sanitaria-Fundación Jiménez Díaz, Madrid, España.
authors.forename: M
authors.initials: M
authors.lastname: García-Arranz
authors.affiliation: Servicio de Cirugía Ortopédica y Traumatología, IDIPAZ, Madrid, España.
authors.forename: S
authors.initials: S
authors.lastname: Antuña
mesh_terms: D000818:Animals; D003131:Combined Modality Therapy; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D011897:Random Allocation; D051381:Rats; D000070636:Rotator Cuff Injuries; D050151:Subcutaneous Fat / Q000166:cytology*; D013708:Tendon Injuries / Q000628:therapy*; D016896:Treatment Outcome
chemical_list: D015718:Fibrin Tissue Adhesive
keywords: Adipose-derived stem-cell; Cell therapy; Celula troncal derivada de lipoaspirado; Experimental; Manguito rotador; Repair; Reparación; Supraspinatus; Terapia celular