title: Ubiquitin ligase CHIP suppresses cancer stem cell properties in a population of breast cancer cells.
abstract: Cancer stem cells (CSCs) have several distinctive characteristics, including high metastatic potential, tumor-initiating potential, and properties that resemble normal stem cells such as self-renewal, differentiation, and drug efflux. Because of these characteristics, CSC is regarded to be responsible for cancer progression and patient prognosis. In our previous study, we showed that a ubiquitin E3 ligase carboxyl terminus of Hsc70-interacting protein (CHIP) suppressed breast cancer malignancy. Moreover, a recent clinical study reported that CHIP expression levels were associated with favorable prognostic parameters of patients with breast cancer. Here we show that CHIP suppresses CSC properties in a population of breast cancer cells. CHIP depletion resulted in an increased proportion of CSCs among breast cancers when using several assays to assess CSC properties. From our results, we propose that inhibition of CSC properties may be one of the functions of CHIP as a suppressor of cancer progression.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Tsuchiya
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan; Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Nakajima
authors.affiliation: Division of Pharmacology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagya-ku, Tokyo 158-8501, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Hirata
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan.
authors.forename: Tamaki
authors.initials: T
authors.lastname: Morishita
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan; Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kishimoto
authors.affiliation: Division of Pharmacology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagya-ku, Tokyo 158-8501, Japan.
authors.forename: Yasunari
authors.initials: Y
authors.lastname: Kanda
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan. Electronic address: kekimura@tara.tsukuba.ac.jp.
authors.forename: Keiji
authors.initials: K
authors.lastname: Kimura
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology*; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*
chemical_list: C467728:STUB1 protein, human; D044767:Ubiquitin-Protein Ligases
keywords: Breast cancer; CHIP; Cancer stem cell