title: Structural, phenotypic and functional maturation of bone marrow dendritic cells (BMDCs) induced by Chitosan (CTS).
abstract: The objective of the present work was to explore the effect of CTS on structural, phenotypic and functional maturation of murine bone marrow derived dendritic cells (BMDCs). The maturity of BMDCs post treatment with CTS was evaluated using transmission electron microscopy (TEM) for structure changes, flow cytometry (FCM) for changes of key surface molecules, FITC-dextran bio-assay for phagocytosis, test of acid phosphatase activity (ACP) for biochemical changes and enzyme linked immunosorbent assay (ELISA) for cytokine level. We found that CTS downregulated the numbers of phagosomes inside the BMDCs, up-regulated the expression of MHC II, CD40, CD83, CD80 and CD86 molecules on BMDCs, decreased activity of ACP and phagocytosis by BMDCs, and induced production of higher levels of IL-12 and TNF-α. It was therefore confirmed that CTS could effectively promote the maturation of BMDCs. Our study provided more detailed evidence and rationale to support the application of CTS as an immune stimulator for enhancing host immunity and as an adjuvant in the design of DC-based vaccines.
authors.affiliation: Department of Stomatology, General Hospital of Shenyang Military Area Command, Shenyang 110084, China.
authors.forename: Lihui
authors.initials: L
authors.lastname: Jia
authors.affiliation: Department of Dermatology, No.1 Hospital, China Medical University, Shenyang 110001, China.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Stomatology, General Hospital of Shenyang Military Area Command, Shenyang 110084, China.
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Stomatology, General Hospital of Shenyang Military Area Command, Shenyang 110084, China.
authors.forename: Na
authors.initials: N
authors.lastname: Yao
authors.affiliation: Department of Stomatology, General Hospital of Shenyang Military Area Command, Shenyang 110084, China. Electronic address: jialihui1959@sina.com.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000135:Acid Phosphatase / Q000737:chemistry; D000818:Animals; D001681:Biological Assay; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D007167:Immunotherapy / Q000379:methods; D018664:Interleukin-12 / Q000378:metabolism; D051379:Mice; D046529:Microscopy, Electron, Transmission; D010587:Phagocytosis; D010641:Phenotype; D013499:Surface Properties; D018417:Th1 Cells / Q000166:cytology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D048271:Chitosan; D000135:Acid Phosphatase
keywords: Bone marrow derived dendritic cells; Chitosan; Immune system; Maturation; Modulation