title: Development of a FI-HG-ICP-OES solid phase preconcentration system for inorganic selenium speciation in Argentinean beverages.
abstract: A preconcentration system has been developed to determine inorganic selenium species. Selenium was retained by a column filled with polyvinyl chloride (PVC) with lanthanum hydroxide co-precipitation. Speciation was achieved by selective photoreduction previous Se preconcentration. The retention pH was optimized at 10.0. Two multivariate calibrations and a central composite design were employed for optimization of the system. Sample, reagents and acid flow rates are significant variables affecting the system. Employing HG-ICP-OES as detection, the optimized system reached a detection limit of 0.03μg/L, and an enhancement factor of 14875 (25 for preconcentration system, 595 for hydride generation). To verify the method' accuracy, two certified reference materials, BCR® 414 Plankton & IRMM-804 Rice Flour, were analysed. The system was applied to inorganic selenium speciation in several Argentinean beverages to estimate their selenium contribution to diet.
authors.affiliation: Instituto de Química de San Luis (INQUISAL-CONICET), Chacabuco y Pedernera, CP 5700 San Luis, Argentina; Área de Química Analítica, Departamento de Química, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, CP D5700BWS San Luis, Argentina.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Escudero
authors.affiliation: Instituto de Química de San Luis (INQUISAL-CONICET), Chacabuco y Pedernera, CP 5700 San Luis, Argentina; Área de Química Analítica, Departamento de Química, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, CP D5700BWS San Luis, Argentina. Electronic address: ppacheco@unsl.edu.ar.
authors.forename: Pablo H
authors.initials: PH
authors.lastname: Pacheco
authors.affiliation: Instituto de Química de San Luis (INQUISAL-CONICET), Chacabuco y Pedernera, CP 5700 San Luis, Argentina; Área de Química Analítica, Departamento de Química, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, CP D5700BWS San Luis, Argentina.
authors.forename: José A
authors.initials: JA
authors.lastname: Gasquez
authors.affiliation: Instituto de Química de San Luis (INQUISAL-CONICET), Chacabuco y Pedernera, CP 5700 San Luis, Argentina.
authors.forename: José A
authors.initials: JA
authors.lastname: Salonia
mesh_terms: D001118:Argentina; D001628:Beverages / Q000032:analysis*; D002138:Calibration; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D012643:Selenium / Q000032:analysis; D018036:Selenium Compounds / Q000032:analysis* / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D018036:Selenium Compounds; D012643:Selenium
keywords: Argentinean beverages; Inorganic selenium species; Multivariate optimization; Polyvinyl chloride (PVC)