title: Comparative proteomic analysis of antagonistic Bacillus amyloliquefaciens Q-426 cultivated under different pH conditions.
abstract: Bacillus amyloliquefaciens Q-426 produces lipopeptide compounds with antifungal activities. Initial pH value has a significant influence on the production of lipopeptide compounds. The correlation between pH and intrinsic mechanism of lipopeptide production was rarely discussed. In this research, comparative proteomics, using two-dimensional gel electrophoresis and mass spectrometry, was applied to identify B. amyloliquefaciens Q-426 intracellular proteins differentially expressed under initial pH 5.0 and 7.3. A total of 24 differential spots (eight downregulated and 16 upregulated) under pH 5.0 were identified. Certain proteins were involved in the regulation of bacillomycin and fengycin production by B. amyloliquefaciens Q-426. These proteins include four induced proteins related to stress response: Thiamine pyrophosphate-dependent acetoin dehydrogenase, butanediol dehydrogenase, two ABC-type oligopeptide transport system proteins, and two-component response regulator DegU and chorismate mutase PheB. These results indicate intrinsic differences of antagonistic B. amyloliquefaciens Q-426 under different pH conditions.
authors.affiliation: Key Laboratory of Biochemical Engineering (State Ethnic Affairs Commission-Ministry of Education), Dalian Nationalities University, Dalian, People's Republic of China.|College of Life Science, Dalian Nationalities University, Dalian, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, People's Republic of China.
authors.forename: Pengchao
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Biochemical Engineering (State Ethnic Affairs Commission-Ministry of Education), Dalian Nationalities University, Dalian, People's Republic of China.|College of Life Science, Dalian Nationalities University, Dalian, People's Republic of China.
authors.forename: Chunshan
authors.initials: C
authors.lastname: Quan
authors.affiliation: Key Laboratory of Biochemical Engineering (State Ethnic Affairs Commission-Ministry of Education), Dalian Nationalities University, Dalian, People's Republic of China.|College of Life Science, Dalian Nationalities University, Dalian, People's Republic of China.
authors.forename: Liming
authors.initials: L
authors.lastname: Jin
authors.affiliation: Key Laboratory of Biochemical Engineering (State Ethnic Affairs Commission-Ministry of Education), Dalian Nationalities University, Dalian, People's Republic of China.|College of Life Science, Dalian Nationalities University, Dalian, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Biochemical Engineering (State Ethnic Affairs Commission-Ministry of Education), Dalian Nationalities University, Dalian, People's Republic of China.|College of Life Science, Dalian Nationalities University, Dalian, People's Republic of China.
authors.forename: Shengdi
authors.initials: S
authors.lastname: Fan
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D040901:Proteomics
chemical_list: D001426:Bacterial Proteins
keywords: Bacillus amyloliquefaciens; lipopeptide; mass spectrometry; pH; proteomic analysis; two-dimensional gel electrophoresis