title: Stem cell senescence. Effects of REAC technology on telomerase-independent and telomerase-dependent pathways.
abstract: Decline in the gene expression of senescence repressor Bmi1, and telomerase, together with telomere shortening, underlay senescence of stem cells cultured for multiple passages. Here, we investigated whether the impairment of senescence preventing mechanisms can be efficiently counteracted by exposure of human adipose-derived stem cells to radio electric asymmetrically conveyed fields by an innovative technology, named Radio Electric Asymmetric Conveyer (REAC). Due to REAC exposure, the number of stem cells positively stained for senescence associated β-galactosidase was significantly reduced along multiple culturing passages. After a 90-day culture, REAC-treated cells exhibited significantly higher transcription of Bmi1 and enhanced expression of other stem cell pluripotency genes and related proteins, compared to unexposed cells. Transcription of the catalytic telomerase subunit (TERT) was also increased in REAC-treated cells at all passages. Moreover, while telomere shortening occurred at early passages in both REAC-treated and untreated cells, a significant rescue of telomere length could be observed at late passages only in REAC-exposed cells. Thus, REAC-asymmetrically conveyed radio electric fields acted on a gene and protein expression program of both telomerase-independent and telomerase-dependent patterning to optimize stem cell ability to cope with senescence progression.
authors.affiliation: 1] Department of Regenerative Medicine, Rinaldi Fontani Institute, Viale Belfiore 43, 50144 Florence, Italy [2] Department of Anti Aging Medicine, Rinaldi Fontani Institute, Viale Belfiore 43, 50144 Florence, Italy [3] Research Department, Rinaldi Fontani Foundation NPO, Viale Belfiore 43, 50144 Florence, Italy [4].
authors.forename: S
authors.initials: S
authors.lastname: Rinaldi
authors.affiliation: 1] Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy [2] Stem Wave Institute for Tissue Healing (SWITH), Gruppo Villa Maria and Ettore Sansavini Health Science Foundation NPO, via Provinciale per Cotignola 9, 48022 Lugo (Ravenna), Italy [3] National Institute of Biostructures and Biosystems at the Department of Experimental, Diagnostic and Specialty Medicine, S. Orsola - Malpighi Hospital, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy [4].
authors.forename: M
authors.initials: M
authors.lastname: Maioli
authors.affiliation: 1] Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy [2] Stem Wave Institute for Tissue Healing (SWITH), Gruppo Villa Maria and Ettore Sansavini Health Science Foundation NPO, via Provinciale per Cotignola 9, 48022 Lugo (Ravenna), Italy.
authors.forename: G
authors.initials: G
authors.lastname: Pigliaru
authors.affiliation: 1] Department of Regenerative Medicine, Rinaldi Fontani Institute, Viale Belfiore 43, 50144 Florence, Italy [2] Department of Anti Aging Medicine, Rinaldi Fontani Institute, Viale Belfiore 43, 50144 Florence, Italy [3] Research Department, Rinaldi Fontani Foundation NPO, Viale Belfiore 43, 50144 Florence, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Castagna
authors.affiliation: 1] Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy [2] Stem Wave Institute for Tissue Healing (SWITH), Gruppo Villa Maria and Ettore Sansavini Health Science Foundation NPO, via Provinciale per Cotignola 9, 48022 Lugo (Ravenna), Italy.
authors.forename: S
authors.initials: S
authors.lastname: Santaniello
authors.affiliation: Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Basoli
authors.affiliation: 1] Department of Regenerative Medicine, Rinaldi Fontani Institute, Viale Belfiore 43, 50144 Florence, Italy [2] Department of Anti Aging Medicine, Rinaldi Fontani Institute, Viale Belfiore 43, 50144 Florence, Italy [3] Research Department, Rinaldi Fontani Foundation NPO, Viale Belfiore 43, 50144 Florence, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Fontani
authors.affiliation: 1] Stem Wave Institute for Tissue Healing (SWITH), Gruppo Villa Maria and Ettore Sansavini Health Science Foundation NPO, via Provinciale per Cotignola 9, 48022 Lugo (Ravenna), Italy [2] National Institute of Biostructures and Biosystems at the Department of Experimental, Diagnostic and Specialty Medicine, S. Orsola - Malpighi Hospital, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Ventura
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects; D015153:Blotting, Western; D002454:Cell Differentiation / Q000528:radiation effects*; D049109:Cell Proliferation; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology / Q000528:radiation effects*; D004558:Electric Stimulation; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D011846:Radio Waves; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*; D016615:Telomere / Q000235:genetics; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; C509186:TERT protein, human; D019098:Telomerase; D001616:beta-Galactosidase