title: Collagen peptide and vitamin C additively attenuate age-related skin atrophy in Sod1-deficient mice.
abstract: Age-related skin thinning is correlated with a decrease in the content of collagen in the skin. Accumulating evidence suggests that collagen peptide (CP) and vitamin C (VC) transcriptionally upregulate type I collagen in vivo. However, the additive effects of CP and VC on age-related skin changes remain unclear. We herein demonstrate that CP and a VC derivative additively corrected age-related skin thinning via reduced oxidative damage in superoxide dismutase 1 (Sod1)-deficient mice. Co-treatment with these compounds significantly normalized the altered gene expression of Col1a1, Has2, and Ci1, a proton-coupled oligopeptide transporter, in Sod1(-/-) skin. The in vitro analyses further revealed that collagen oligopeptide, a digestive product of ingested CP, significantly promoted the bioactivity of the VC derivative with respect to the migration and proliferation of Sod1(-/-) fibroblasts. These findings suggest that combined treatment with CP and VC is effective in cases of age-related skin pathology.
authors.affiliation: a Department of Advanced Aging Medicine , Chiba University Graduate School of Medicine , Chiba , Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Shibuya
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ozawa
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Toda
authors.forename: Kenji
authors.initials: K
authors.lastname: Watanabe
authors.forename: Chisa
authors.initials: C
authors.lastname: Tometsuka
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ogura
authors.forename: Yoh-ichi
authors.initials: Y
authors.lastname: Koyama
authors.forename: Takahiko
authors.initials: T
authors.lastname: Shimizu
mesh_terms: D000375:Aging / Q000473:pathology*; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology* / Q000627:therapeutic use; D001284:Atrophy / Q000188:drug therapy; D003094:Collagen / Q000737:chemistry*; D004357:Drug Synergism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D010446:Peptide Fragments / Q000494:pharmacology* / Q000627:therapeutic use; D010641:Phenotype; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D013482:Superoxide Dismutase / Q000172:deficiency*; D000072105:Superoxide Dismutase-1; D059467:Transcriptome / Q000187:drug effects
chemical_list: D010446:Peptide Fragments; D003094:Collagen; C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D001205:Ascorbic Acid
keywords: collagen peptide; proton-coupled oligopeptide transporter; skin atrophy; superoxide dismutase; vitamin C