title: Circulating rotavirus-specific T cells have a poor functional profile.
abstract: Frequencies of circulating T cells producing IFN-γ, TNF-α, and IL-2, and percentages of T cells proliferating after stimulation with rotavirus (RV), tetanus toxoid, and influenza were evaluated in PBMC derived from healthy adults and children. In addition, the potential anergic state of RV-specific T cells was analyzed by stimulation of PBMC with RV antigen in the presence of three anergy inhibitors (rIL-2, rIL-12, or DGKα-i). The quality and magnitude of RV-T cell responses were significantly lower than those of tetanus toxoid and influenza antigens. RV-CD4 T cell response was enriched in monofunctional IFN-γ(+) cells, while influenza-CD4 and tetanus toxoid-CD4 T cell responses were enriched in multifunctional T cells. Moreover, rIL-2--unlike rIL-12 or DGKα-i--increased the frequencies of RV-CD4 TNF-α(+), CD4 IFN-γ(+), and CD8 IFN-γ(+) cells. Thus, circulating RV-T cells seem to have a relatively poor functional profile that may be partially reversed in vitro by the addition of rIL-2.
authors.affiliation: Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Pontificia Universidad Javeriana, Carrera 7 # 40-62, Bogotá, Colombia.
authors.forename: Miguel
authors.initials: M
authors.lastname: Parra
authors.affiliation: Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Pontificia Universidad Javeriana, Carrera 7 # 40-62, Bogotá, Colombia.
authors.forename: Daniel
authors.initials: D
authors.lastname: Herrera
authors.affiliation: Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: María Fernanda
authors.initials: MF
authors.lastname: Jácome
authors.affiliation: Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Martha C
authors.initials: MC
authors.lastname: Mesa
authors.affiliation: Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Pontificia Universidad Javeriana, Carrera 7 # 40-62, Bogotá, Colombia.
authors.forename: Luz-Stella
authors.initials: LS
authors.lastname: Rodríguez
authors.affiliation: Departamento de Pediatría, Hospital Universitario San Ignacio, Facultad de Medicina, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Carolina
authors.initials: C
authors.lastname: Guzmán
authors.affiliation: Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Pontificia Universidad Javeriana, Carrera 7 # 40-62, Bogotá, Colombia.
authors.forename: Juana
authors.initials: J
authors.lastname: Angel
authors.affiliation: Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Pontificia Universidad Javeriana, Carrera 7 # 40-62, Bogotá, Colombia.
authors.forename: Manuel A
authors.initials: MA
authors.lastname: Franco
mesh_terms: D000328:Adult; D049109:Cell Proliferation; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D007252:Influenza Vaccines; D008875:Middle Aged; D012401:Rotavirus / Q000276:immunology / Q000502:physiology*; D012400:Rotavirus Infections / Q000276:immunology / Q000821:virology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D013745:Tetanus Toxoid; D055815:Young Adult
chemical_list: D016207:Cytokines; D007252:Influenza Vaccines; D013745:Tetanus Toxoid
keywords: Cytokines; Proliferation; Rotavirus (RV); T cells