title: Structural characterisation, physicochemical properties and antioxidant activity of polysaccharide from Lilium lancifolium Thunb.
abstract: A novel polysaccharide fraction (LP2-1) was isolated and purified from the edible bulbs of Lilium lancifolium Thunb. by DEAE cellulose chromatography and Sephacryl S-400 size-exclusion chromatography. The structural characterisation, physicochemical properties and antioxidant activity of LP2-1 were investigated. The results showed that LP2-1 had an average molecular weight of approximately 8.52×10(3)kDa and was mainly composed of l-rhamnopyranose, d-arabinofuranose, d-glucopyranose and d-galactopyranose in the molar ratio of 1.88:2.13:1.00:2.50, and major functional groups of LP2-1 were COO and OH. The viscoelastic properties of LP2-1 systems exhibited a gel-like behaviour, with storage modulus higher than loss modulus, and both moduli increased with increasing Ca(2+) concentration. In addition, LP2-1 had DPPH and hydroxyl radicals scavenging activities, and also had the strong reducing power and chelating activity on ferrous ion. These results suggest that LP2-1 has good antioxidant activity and can be used in food industry.
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China.
authors.forename: Zheng-Yu
authors.initials: ZY
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China.
authors.forename: Xue-Ming
authors.initials: XM
authors.lastname: Xu
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China.
authors.forename: Jun-Hui
authors.initials: JH
authors.lastname: Wang
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China.
authors.forename: Xue-Qiang
authors.initials: XQ
authors.lastname: Zha
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China. Electronic address: hanqchen@hfut.edu.cn.
authors.forename: Han-Qing
authors.initials: HQ
authors.lastname: Chen
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002850:Chromatography, Gel; D027762:Lilium / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D014574:Uronic Acids / Q000032:analysis
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D014574:Uronic Acids
keywords: Antioxidant activity; Lilium lancifolium Thunb.; Physicochemical property; Polysaccharide; Structure