title: Chemical and genetic studies on hybrid of Ligularia subspicata and Ligularia cyathiceps collected in Yunnan Province of China.
abstract: Two morphologically ambiguous Ligularia samples (samples A and B), and samples with morphology of Ligularia subspicata (sample C), Ligularia lamarum (sample D), or Ligularia cyathiceps (sample E), were collected at Tianchi Pond, Shangrila County, Yunnan Province, China. Analysis of the nucleotide sequence of the internal transcribed spacers (ITSs) in the nuclear ribosomal RNA gene cluster indicated that not only sample B but also sample D was a hybrid of L. cyathiceps and L. lamarum/L. subspicata. Although the morphology of sample A suggested that it was also a hybrid, the ITS sequence of sample A was that of L. cyathiceps. Twenty compounds were isolated from the five samples, and the structures of two new compounds 7 and 14 were determined. Furanoeremophilanes typical of L. lamarum/L. subspicata were detected not only in samples C and D, but also in samples A and B. These results indicate that the ability to produce root chemicals can spread through introgression.
authors.affiliation: Department of Chemistry and Research Center for Smart Molecules, Rikkyo University, Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, Japan, (phone/fax: +81-3-39852396).
authors.forename: Anna
authors.initials: A
authors.lastname: Shimizu
authors.forename: Shizuka
authors.initials: S
authors.lastname: Watanabe
authors.forename: Ryo
authors.initials: R
authors.lastname: Hanai
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Okamoto
authors.forename: Motoo
authors.initials: M
authors.lastname: Tori
authors.forename: Xun
authors.initials: X
authors.lastname: Gong
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kuroda
mesh_terms: D019659:Asteraceae / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D002681:China; D017343:Genes, Plant; D008969:Molecular Sequence Data; D012335:RNA, Ribosomal / Q000235:genetics; D013045:Species Specificity
chemical_list: D012335:RNA, Ribosomal
keywords: Furanoeremophilanes; Internal transcribed spacer (ITS); Ligularia