title: Cysteine proteinase genes in Indian strain of Haemonchus contortus.
abstract: The genes of microvillar cysteine proteinases such as hmcp1, hmcp6 and gcp7 of phenotypically distinct morphotypes of female H. contortus having knobbed and linguiform vulvar flaps were cloned and characterized. Complementary DNA (cDNA) was synthesized from total RNA of both the morphotypes. The hmcp1, hmcp6 and gcp7 genes were amplified from cDNA. The amplicons were cloned in pDRIVE T/A cloning vector and sequenced by Sanger's dideoxy method. The nucleotide and deduced amino acid sequences were compared with the USA/UK strain H. contortus cysteine proteinase sequences. The cysteine proteinase genes of Indian strain show considerable variations with USA/UK strains. Our results revealed that Indian strain of H. contortus expresses both UK strain (hmcp1 and 6) and USA strain (gcp7) cysteine proteinases.
authors.affiliation: Network Programme on GI Parasitism, Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India. Electronic address: drarvindivri@rediffmail.com.
authors.forename: I M
authors.initials: IM
authors.lastname: Allaie
authors.affiliation: Network Programme on GI Parasitism, Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
authors.forename: A
authors.initials: A
authors.lastname: Prasad
authors.affiliation: Network Programme on GI Parasitism, Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
authors.forename: M
authors.initials: M
authors.lastname: Sankar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D057056:Cysteine Proteases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006188:Haemonchiasis / Q000469:parasitology / Q000662:veterinary*; D006189:Haemonchus / Q000737:chemistry / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007194:India; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology*
chemical_list: D015801:Helminth Proteins; D057056:Cysteine Proteases
keywords: Cysteine proteinase; Haemonchus contortus; Knobbed; Linguiform; gcp7; hmcp1; hmcp6