title: Application of MALDI-TOF MS for requalification of a Candida clinical isolates culture collection.
abstract: Microbial culture collections underpin biotechnology applications and are important resources for clinical microbiology by supplying reference strains and/or performing microbial identifications as a service. Proteomic profiles by MALDI-TOF MS have been used for Candida spp. identification in clinical laboratories and demonstrated to be a fast and reliable technique for the routine identification of pathogenic yeasts. The main aim of this study was to apply MALDI-TOF MS combined with classical phenotypic and molecular approaches to identify Candida clinical isolates preserved from 1 up to 52 years in a Brazilian culture collection and assess its value for the identification of yeasts preserved in this type of collections. Forty Candida spp. clinical isolates were identified by morphological and biochemical analyses. Identifications were also performed by the new proteomic approach based on MALDI-TOF MS. Results demonstrated 15% discordance when compared with morphological and biochemical analyses. Discordant isolates were analysed by ITS sequencing, which confirmed the MALDI-TOF MS identifications and these strains were renamed in the culture collection catalogue. In conclusion, proteomic profiles by MALDI-TOF MS represents a rapid and reliable method for identifying clinical Candida species preserved in culture collections and may present clear benefits when compared with the performance of existing daily routine methods applied at health centres and hospitals.
authors.affiliation: Department of Mycology Centre of Biological Sciences Federal University of Pernambuco RecifePE Brazil Department of Mycology, Centre of Biological Sciences, Federal University of Pernambuco, Recife, PE, Brazil. ; Micoteca da Universidade do Minho Centre of Biological Engineering University of Minho Braga Portugal Micoteca da Universidade do Minho, Centre of Biological Engineering, University of Minho, Braga, Portugal.
authors.forename: Reginaldo
authors.initials: R
authors.lastname: Lima-Neto
authors.affiliation: Micoteca da Universidade do Minho Centre of Biological Engineering University of Minho Braga Portugal Micoteca da Universidade do Minho, Centre of Biological Engineering, University of Minho, Braga, Portugal.
authors.forename: Cledir
authors.initials: C
authors.lastname: Santos
authors.affiliation: Micoteca da Universidade do Minho Centre of Biological Engineering University of Minho Braga Portugal Micoteca da Universidade do Minho, Centre of Biological Engineering, University of Minho, Braga, Portugal.
authors.forename: Nelson
authors.initials: N
authors.lastname: Lima
authors.affiliation: Department of Biology Centre of Molecular and Environmental Biology University of Minho Braga Portugal Department of Biology, Centre of Molecular and Environmental Biology, University of Minho, Braga, Portugal.
authors.forename: Paula
authors.initials: P
authors.lastname: Sampaio
authors.affiliation: Department of Biology Centre of Molecular and Environmental Biology University of Minho Braga Portugal Department of Biology, Centre of Molecular and Environmental Biology, University of Minho, Braga, Portugal.
authors.forename: Célia
authors.initials: C
authors.lastname: Pais
authors.affiliation: Department of Mycology Centre of Biological Sciences Federal University of Pernambuco RecifePE Brazil Department of Mycology, Centre of Biological Sciences, Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Rejane P
authors.initials: RP
authors.lastname: Neves
mesh_terms: D001938:Brazil; D002175:Candida / Q000737:chemistry* / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002177:Candidiasis / Q000382:microbiology; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer
keywords: BRCs; Candida; MALDI-TOF MS; clinical yeasts; culture collections