title: Adipose-derived stem cells (ADSC) in the viability of random skin flap in rats.
abstract: OBJECTIVE
  To evaluate the effects of the adipose-derived stem cells (ADSC) in the viability of random skin flap in rats.
  
  
  METHODS
  Thirty five adult male Wistar rats (weight 250-300 g) were used. ADSC were isolated from adult male rats (n=5). ADSC were separated, cultured and then analyzed. A dorsal skin flap measuring 10 x 4 cm was raised and a plastic barrier was placed between the flap and its bed. After the surgical procedure, the animals were randomized into two groups (n=15 each group), group control and group ADSC. In all groups the procedures were performed immediately after the surgery. The percentage of flap necrosis was measured on the seventh postoperative day.
  
  
  RESULTS
  The ADSC were able to replicate in our culture conditions. We also induced their adipogenic, osteogenic and chondrogenic differentiation, verifying their mesenchymal stem cells potentiality in vitro. The results were statistically significant showing that the ADSC decreased the area of necrosis (p<0.05).
  
  
  CONCLUSIONS
  The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro. The administration of adipose-derived stem cells was effective to increase the viability of the random skin flaps in rats.
authors.affiliation: Department of Surgery, Federal University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Caio Vinicius
authors.initials: CV
authors.lastname: Suartz
authors.affiliation: Department of Surgery, Federal University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Silvana
authors.initials: S
authors.lastname: Gaiba
authors.affiliation: Department of Biological Sciences, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil.
authors.forename: Jerônimo Pereira de
authors.initials: JP
authors.lastname: França
authors.affiliation: Department of Surgery, Federal University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Antonio Carlos
authors.initials: AC
authors.lastname: Aloise
authors.affiliation: UNIFESP, Sao Paulo, SP, Brazil.
authors.forename: Lydia Masako
authors.initials: LM
authors.lastname: Ferreira
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D006085:Graft Survival / Q000502:physiology*; D008297:Male; D009336:Necrosis; D011897:Random Allocation; D017208:Rats, Wistar; D012867:Skin / Q000473:pathology*; D013234:Stem Cells / Q000502:physiology*; D013524:Surgical Flaps / Q000473:pathology / Q000502:physiology*; D014022:Tissue Survival / Q000502:physiology