title: Self-regulated left-right asymmetric expression of Pitx2c in the developing mouse limb.
abstract: The transcription factor Pitx2c is expressed in primordial visceral organs in a left-right (L-R) asymmetric manner and executes situs-specific morphogenesis. Here we show that Pitx2c is also L-R asymmetrically expressed in the developing mouse limb. Human PITX2c exhibits the same transcriptional activity in the mouse limb. The asymmetric expression of Pitx2c in the limb also exhibits dorsal-ventral and anterior-posterior polarities, being confined to the posterior-dorsal region of the left limb. Left-sided Pitx2c expression in the limb is regulated by Nodal signaling through a Nodal-responsive enhancer. Pitx2c is expressed in lateral plate mesoderm (LPM)-derived cells in the left limb that contribute to various limb connective tissues. The number of Pitx2c(+) cells in the left limb was found to be negatively regulated by Pitx2c itself. Although obvious defects were not apparent in the limb of mice lacking asymmetric Pitx2c expression, Pitx2c may regulate functional L-R asymmetry of the limb.
authors.affiliation: Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, and CREST, Japan Science and Technology Corporation (JST), 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan. Electronic address: shiratori@fbs.osaka-u.ac.jp.
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Shiratori
authors.affiliation: Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, and CREST, Japan Science and Technology Corporation (JST), 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Yashiro
authors.affiliation: Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, and CREST, Japan Science and Technology Corporation (JST), 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Iwai
authors.affiliation: Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, and CREST, Japan Science and Technology Corporation (JST), 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Oki
authors.affiliation: Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, and CREST, Japan Science and Technology Corporation (JST), 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Katsura
authors.initials: K
authors.lastname: Minegishi
authors.affiliation: Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, and CREST, Japan Science and Technology Corporation (JST), 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Ikawa
authors.affiliation: Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, and CREST, Japan Science and Technology Corporation (JST), 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Kanata
authors.affiliation: Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, and CREST, Japan Science and Technology Corporation (JST), 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hamada
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D005121:Extremities / Q000196:embryology*; D005455:Fluorescent Antibody Technique; D005697:Galactosides; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D055879:Gene Knock-In Techniques; D018398:Homeodomain Proteins / Q000378:metabolism*; D017403:In Situ Hybridization; D007211:Indoles; D051379:Mice; D008822:Mice, Transgenic; D009024:Morphogenesis / Q000502:physiology*; D013629:Tamoxifen; D014157:Transcription Factors / Q000378:metabolism*; D000097577:Homeobox Protein PITX2
chemical_list: D017931:DNA Primers; D005697:Galactosides; D018398:Homeodomain Proteins; D007211:Indoles; D013629:Tamoxifen; D014157:Transcription Factors; D000097577:Homeobox Protein PITX2; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside
keywords: Left-right asymmetry; Limb; Pitx2