title: Comprehensive analysis of stop codon usage in bacteria and its correlation with release factor abundance.
abstract: We present a comprehensive analysis of stop codon usage in bacteria by analyzing over eight million coding sequences of 4684 bacterial sequences. Using a newly developed program called "stop codon counter," the frequencies of the three classical stop codons TAA, TAG, and TGA were analyzed, and a publicly available stop codon database was built. Our analysis shows that with increasing genomic GC content the frequency of the TAA codon decreases and that of the TGA codon increases in a reciprocal manner. Interestingly, the release factor 1-specific codon TAG maintains a more or less uniform frequency (∼20%) irrespective of the GC content. The low abundance of TAG is also valid with respect to expression level of the genes ending with different stop codons. In contrast, the highly expressed genes predominantly end with TAA, ensuring termination with either of the two release factors. Using three model bacteria with different stop codon usage (Escherichia coli, Mycobacterium smegmatis, and Bacillus subtilis), we show that the frequency of TAG and TGA codons correlates well with the relative steady state amount of mRNA and protein for release factors RF1 and RF2 during exponential growth. Furthermore, using available microarray data for gene expression, we show that in both fast growing and contrasting biofilm formation conditions, the relative level of RF1 is nicely correlated with the expression level of the genes ending with TAG.
authors.affiliation: Department of Cell and Molecular Biology, Uppsala University, Box-596, BMC, Uppsala S-75124, Sweden.
authors.forename: Gürkan
authors.initials: G
authors.lastname: Korkmaz
authors.affiliation: Department of Cell and Molecular Biology, Uppsala University, Box-596, BMC, Uppsala S-75124, Sweden.
authors.forename: Mikael
authors.initials: M
authors.lastname: Holm
authors.affiliation: Department of Cell and Molecular Biology, Uppsala University, Box-596, BMC, Uppsala S-75124, Sweden.
authors.forename: Tobias
authors.initials: T
authors.lastname: Wiens
authors.affiliation: Department of Cell and Molecular Biology, Uppsala University, Box-596, BMC, Uppsala S-75124, Sweden. Electronic address: suparna.sanyal@icm.uu.se.
authors.forename: Suparna
authors.initials: S
authors.lastname: Sanyal
mesh_terms: D001419:Bacteria / Q000235:genetics*; D001482:Base Composition; D018388:Codon, Terminator; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D017422:Sequence Analysis, DNA
chemical_list: D018388:Codon, Terminator
keywords: Bacteria; Codon Usage; Protein Synthesis; Ribosome; Stop Codon; Translation; Translation Release Factor