title: Survival of tissue balls from the coral Pocillopora damicornis L. exposed to cryoprotectant solutions.
abstract: In this study, the tolerance of tissue balls (TBs, 100-300 μm in diameter) from the coral Pocillopora damicornis produced using mechanical excision to exposure to cryoprotectant (CPA) solutions was tested. TBs were treated for 20 min at room temperature with solutions of ethylene glycol (EG), methanol (Met), glycerol (Gly) or dimethyl sulfoxide (Me2SO) at concentrations between 1.0 and 4.5M. Two parameters were used to evaluate the survival of TBs following CPA treatment. The Undamaged Duration of Tissue Balls (expressed in h) corresponded to the time period during which the membrane surface of TBs remained smooth and their motility was preserved. Tissue Ball Regression (expressed in μm/h) corresponded to the size reduction of TBs over time. TBs tolerated exposure to all CPAs tested at the three lower concentrations employed (1.0 M, 1.5 M and 2.0 M). No survival was achieved following exposure to a 4.5 M CPA solution. At concentrations of 3.0 and 4.0 M, higher Undamaged Duration of Tissue Balls and lower Tissue Ball Regression were obtained following treatment with EG compared to the other three CPAs. Our experiments show that TBs constitute a good experimental material to evaluate CPA toxicity on corals using large numbers of samples. Performing preliminary experiments with TBs may allow reducing the number of tests carried out with less easily available coral forms such as planulae, thereby preserving larval stocks.
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMS 2348, Centre de Ressources Biologiques Marines, Observatoire Océanologique de Banyuls sur mer, F-75005 Paris, France; IRD, UMR DIADE, 911 Avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Feuillassier
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMS 2348, Centre de Ressources Biologiques Marines, Observatoire Océanologique de Banyuls sur mer, F-75005 Paris, France.
authors.forename: Lucie
authors.initials: L
authors.lastname: Martinez
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMS 2348, Centre de Ressources Biologiques Marines, Observatoire Océanologique de Banyuls sur mer, F-75005 Paris, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Romans
authors.affiliation: IRD, UMR DIADE, 911 Avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Engelmann-Sylvestre
authors.affiliation: Aquarium de Canet-en-Roussillon, 2 boulevard de la Jetée, 66140 Canet-en-Roussillon, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Masanet
authors.affiliation: Océanopolis, Port de plaisance du Moulin Blanc, BP91039, 29210 Brest Cedex 1, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Barthélémy
authors.affiliation: IRD, UMR DIADE, 911 Avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France. Electronic address: florent.engelmann@ird.fr.
authors.forename: Florent
authors.initials: F
authors.lastname: Engelmann
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000633:toxicity*; D004121:Dimethyl Sulfoxide / Q000633:toxicity; D019855:Ethylene Glycol / Q000633:toxicity; D005990:Glycerol / Q000633:toxicity; D000432:Methanol / Q000633:toxicity; D019946:Propylene Glycol / Q000633:toxicity
chemical_list: D003451:Cryoprotective Agents; D019946:Propylene Glycol; D019855:Ethylene Glycol; D005990:Glycerol; D000432:Methanol; D004121:Dimethyl Sulfoxide
keywords: Coral; Cryopreservation; Cryoprotectant; Pocillopora damicornis; Tissue ball