title: First report of Corynebacterium pseudotuberculosis from caseous lymphadenitis lesions in Black Alentejano pig (Sus scrofa domesticus).
abstract: BACKGROUND
  Corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis, a common disease in small ruminant populations throughout the world and responsible for a significant economic impact for producers.
  
  
  METHODS
  To our knowledge, this is the first characterization of C. pseudotuberculosis from caseous lymphadenitis lesions in Black Alentejano pig (Sus scrofa domesticus). In this study, phenotypic and genotypic identification methods allocated the swine isolates in C. pseudotuberculosis biovar ovis. The vast majority of the isolates were able to produce phospholipase D and were susceptible to most of the antimicrobial compounds tested. Macrorestriction patterns obtained by Pulsed Field Gel Electrophoresis (PFGE) grouped the C. pseudotuberculosis in two clusters with a high similarity index, which reveals their clonal relatedness. Furthermore, swine isolates were compared with C. pseudotuberculosis from caprines and PFGE patterns also showed high similarity, suggesting the prevalence of dominant clones and a potential cross-dissemination between these two animal hosts.
  
  
  CONCLUSIONS
  This work represents the first report of Corynebacterium pseudotuberculosis from caseous lymphadenitis lesions in Black Alentejano pig and alerts for the importance of the establishment of suitable control and sanitary management practices to control the infection and avoid further dissemination of this important pathogen to other animal hosts.
authors.forename: Manuela
authors.initials: M
authors.lastname: Oliveira
authors.forename: Cynthia
authors.initials: C
authors.lastname: Barroco
authors.forename: Carla
authors.initials: C
authors.lastname: Mottola
authors.forename: Raquel
authors.initials: R
authors.lastname: Santos
authors.forename: Abdelhak
authors.initials: A
authors.lastname: Lemsaddek
authors.forename: Luis
authors.initials: L
authors.lastname: Tavares
authors.forename: Teresa
authors.initials: T
authors.lastname: Semedo-Lemsaddek
mesh_terms: D000038:Abscess / Q000382:microbiology / Q000473:pathology / Q000662:veterinary; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003354:Corynebacterium Infections / Q000382:microbiology / Q000662:veterinary*; D016925:Corynebacterium pseudotuberculosis / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D024881:Drug Resistance, Bacterial; D008199:Lymphadenitis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents