title: Colloidal gold nanoparticle conjugates of gefitinib.
abstract: Gefitinib (GF) is a US Food and Drug Administration-approved epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitor for treating the lung cancers. We fabricated colloidal gold nanoparticle (AuNP) conjugates of the GF anticancer drug by self-assembly to test their potency against A549, NCI-H460, and NCI-H1975 lung cancer cells. GF adsorption on AuNP surfaces was examined by UV-vis absorption spectra and surface-enhanced Raman scattering. Density functional theory calculations were performed to estimate the energetic stabilities of the drug-AuNP composites. The N1 nitrogen atom of the quinazoline ring of GF was calculated to be more stable than the N3 in binding Au cluster atoms. The internalizations of GF-coated AuNPs were examined by transmission electron and dark-field microscopy. A cell viability test of AuNP-GF conjugates with the EGFR antibody exhibited much higher reductions than free GF for A549, NCI-H460, and NCI-H1975 lung cancer cells after treatment for 48.
authors.affiliation: Department of Chemistry, Soongsil University, Seoul 156-743, South Korea.
authors.forename: Anh Thu Ngoc
authors.initials: AT
authors.lastname: Lam
authors.affiliation: Department of Chemistry, Soongsil University, Seoul 156-743, South Korea.
authors.forename: Jinha
authors.initials: J
authors.lastname: Yoon
authors.affiliation: Department of Chemistry, Soongsil University, Seoul 156-743, South Korea.
authors.forename: Erdene-Ochir
authors.initials: EO
authors.lastname: Ganbold
authors.affiliation: Department of Physics, Sogang University, Seoul 121-742, South Korea.
authors.forename: Dheeraj K
authors.initials: DK
authors.lastname: Singh
authors.affiliation: Department of Physics, Sogang University, Seoul 121-742, South Korea.
authors.forename: Doseok
authors.initials: D
authors.lastname: Kim
authors.affiliation: School of Systems Biomedical Science, Soongsil University, Sangdo-dong, Dongjak-gu, Seoul, South Korea.
authors.forename: Kwang-Hwi
authors.initials: KH
authors.lastname: Cho
authors.affiliation: College of Veterinary Medicine, Seoul National University, Seoul 156-743, South Korea.
authors.forename: So Yeong
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Bionano Technology, Hanyang University, Sa-1-dong 1271, Ansan 426-791, South Korea.
authors.forename: Jaebum
authors.initials: J
authors.lastname: Choo
authors.affiliation: Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, South Korea.
authors.forename: Kangtaek
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Soongsil University, Seoul 156-743, South Korea. Electronic address: sjoo@ssu.ac.kr.
authors.forename: Sang-Woo
authors.initials: SW
authors.lastname: Joo
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D066246:ErbB Receptors / Q000737:chemistry; D000077156:Gefitinib; D017979:Gold Colloid / Q000737:chemistry*; D006801:Humans; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000737:chemistry*; D011799:Quinazolines / Q000737:chemistry* / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013059:Spectrum Analysis, Raman
chemical_list: D017979:Gold Colloid; D008567:Membranes, Artificial; D011799:Quinazolines; D012710:Serum Albumin, Bovine; D066246:ErbB Receptors; D000077156:Gefitinib
keywords: Cell viability; Gefitinib; Gold nanoparticle conjugates; Lung cancer cells; Surface-enhanced Raman scattering