title: A novel Bruch's membrane-mimetic electrospun substrate scaffold for human retinal pigment epithelium cells.
abstract: Various artificial membranes have been used as scaffolds for retinal pigment epithelium cells (RPE) for monolayer reconstruction, however, long-term cell viability and functionality are still largely unknown. This study aimed to construct an ultrathin porous nanofibrous film to mimic Bruch's membrane, and in particular to investigate human RPE cell responses to the resultant substrates. An ultrathin porous nanofibrous membrane was fabricated by using regenerated wild Antheraea pernyi silk fibroin (RWSF), polycaprolactone (PCL) and gelatin (Gt) and displayed a thickness of 3-5 μm, with a high porosity and an average fiber diameter of 166 ± 85 nm. Human RPE cells seeded on the RWSF/PCL/Gt membranes showed a higher cell growth rate (p < 0.05), and a typical expression pattern of RPE signature genes, with reduced expression of inflammatory mediators. With long-term cultivation on the substrates, RPE cells exhibited characteristic polygonal morphology and development of apical microvilli. Immunocytochemisty demonstrated RPE-specific expression profiles in cells after 12-weeks of co-culture on RWSF/PCL/Gt membranes. Interestingly, the cells on the RWSF/PCL/Gt membranes functionally secreted polarized PEDF and phagocytosed labeled porcine POS. Furthermore, RWSF/PCL/Gt membranes transplanted subsclerally exhibited excellent biocompatibility without any evidence of inflammation or rejection. In conclusion, we established a novel RWSF-based substrate for growth of RPE cells with excellent cytocompatibility in vitro and biocompatibility in vivo for potential use as a prosthetic Bruch's membrane for RPE transplantation.
authors.affiliation: Laboratory for Stem Cell & Retinal Regeneration, The Eye Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Xiang
authors.affiliation: Laboratory for Stem Cell & Retinal Regeneration, The Eye Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Kun-Chao
authors.initials: KC
authors.lastname: Wu
authors.affiliation: College of Life Science, Fujian Normal University, Fuzhou 350108, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Laboratory for Stem Cell & Retinal Regeneration, The Eye Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Lue
authors.initials: L
authors.lastname: Xiang
authors.affiliation: Tongji Eye Institute, Tongji University School of Medicine, Shanghai 200092, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Life Science, Fujian Normal University, Fuzhou 350108, China.
authors.forename: Deng-Long
authors.initials: DL
authors.lastname: Chen
authors.affiliation: Laboratory for Stem Cell & Retinal Regeneration, The Eye Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: Tongji Eye Institute, Tongji University School of Medicine, Shanghai 200092, China.
authors.forename: Guotong
authors.initials: G
authors.lastname: Xu
authors.affiliation: Surgical Bioengineering Laboratory, University of California Davis School of Medicine, CA 95817, USA.
authors.forename: Aijun
authors.initials: A
authors.lastname: Wang
authors.affiliation: College of Life Science, Fujian Normal University, Fuzhou 350108, China. Electronic address: mli@fjnu.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Laboratory for Stem Cell & Retinal Regeneration, The Eye Hospital of Wenzhou Medical University, Wenzhou 325027, China. Electronic address: jinzb@mail.eye.ac.cn.
authors.forename: Zi-Bing
authors.initials: ZB
authors.lastname: Jin
mesh_terms: D000328:Adult; D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry; D016570:Bruch Membrane / Q000737:chemistry*; D002478:Cells, Cultured; D005349:Fibroins / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D009036:Moths / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D011817:Rabbits; D055213:Retinal Pigment Epithelium / Q000166:cytology* / Q000378:metabolism; D013552:Swine; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; C016240:polycaprolactone; D005780:Gelatin; D005349:Fibroins
keywords: Bruch's membrane; Cytocompatibility; Electrospinning; RPE; Regenerated wild Antheraea pernyi silk fibroin