title: Comparison of the lipid-lowering effects of four different n-3 highly unsaturated fatty acids in HepG2 cells.
abstract: The effects on lipid metabolism of four different n-3 highly unsaturated fatty acids (n-3HUFA) including eicosapentaenoic acid (EPA, 20:5n-3), docosapentaenoic acid (DPA, 22:5n-3), docosahexaenoic acid (DHA, 22:6n-3), and tetracosahexaenoic acid (THA, 24:6n-3) were compared in the HepG2 cell model. None of the n-3HUFAs affected the viability of the cells. THA exerted the strongest suppression on the synthesis of triacylglycerol and cholesteryl ester (ChE), and the order of the strength of suppression was found to be THA > DHA > DPA > EPA. The mRNA level of fatty acid synthase was suppressed by the n-3HUFAs and the order of the strength of suppression by n-3HUFAs was the same in both triacylglycerol and ChE synthesis. These findings support previous animal test results using EPA, DPA, and DHA. In conclusion, both the number of carbon atoms and double bonds in an n-3HUFA structure has an effect on lipid metabolism in HepG2 cells.
authors.affiliation: Department of Applied Biochemistry and Food Science, Saga University.
authors.forename: Koji
authors.initials: K
authors.lastname: Nagao
authors.forename: Kazusa
authors.initials: K
authors.lastname: Nakamitsu
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ishida
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Yoshinaga
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Nagai
authors.forename: Hoyo
authors.initials: H
authors.lastname: Mizobe
authors.forename: Koichi
authors.initials: K
authors.lastname: Kojima
authors.forename: Teruyoshi
authors.initials: T
authors.lastname: Yanagita
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Beppu
authors.forename: Naohiro
authors.initials: N
authors.lastname: Gotoh
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002788:Cholesterol Esters / Q000096:biosynthesis; D003864:Depression, Chemical; D004281:Docosahexaenoic Acids / Q000737:chemistry / Q000494:pharmacology; D015118:Eicosapentaenoic Acid / Q000737:chemistry / Q000494:pharmacology; D064429:Fatty Acid Synthases / Q000235:genetics / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000737:chemistry / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D013329:Structure-Activity Relationship; D014280:Triglycerides / Q000096:biosynthesis
chemical_list: D002788:Cholesterol Esters; D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated; D012333:RNA, Messenger; D014280:Triglycerides; D004281:Docosahexaenoic Acids; C099831:tetracosahexaenoic acid; D015118:Eicosapentaenoic Acid; D064429:Fatty Acid Synthases; C026219:docosapentaenoic acid