title: Biotransformation of 1,3-propanediol cyclic sulfate and its derivatives to diols by Rhodococcus sp.
abstract: Rhodococcus sp. CGMCC 4911 transformed 1,3-propanediol cyclic sulfate (1,3-PDS) and its derivatives into corresponding diols. Ethylene sulfate, glycol sulfide, 1,3-PDS, and 1,2-propanediol cyclic sulfate were effectively hydrolyzed with growing cells. (R)-1,2-Propanediol (>99 % e.e.) was obtained at 44 % yield with growing cells. Glycol sulfide, ethylene sulfate, and 1,3-PDS were converted into the corresponding diols at 94.6, 96.3, and 98.3 %, respectively. Optimal reaction conditions with lyophilized resting cells were 30 °C, pH 7.5, and cell dosage 17.9 mg cell dry wt/ml. 1,3-Propanediol was obtained from 50 mM 1,3-PDS at 97.2 % yield by lyophilized cells after 16 h. Lyophilized cells were entrapped in calcium alginate with a half-life of 263 h at 30 °C, and the total operational time of the immobilized biocatalysts could reach over 192 h with a high conversion rate.
authors.affiliation: Laboratory of Biocatalysis and Bioprocessing, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, 213164, People's Republic of China, heyucai2001@163.com.
authors.forename: Yu-Cai
authors.initials: YC
authors.lastname: He
authors.forename: Zhi-Cheng
authors.initials: ZC
authors.lastname: Tao
authors.forename: Dan-Ping
authors.initials: DP
authors.lastname: Zhang
authors.forename: Zhen-Xing
authors.initials: ZX
authors.lastname: Yang
authors.forename: Shan
authors.initials: S
authors.lastname: Gao
authors.forename: Cui-Luan
authors.initials: CL
authors.lastname: Ma
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001709:Biotechnology; D001711:Biotransformation; D018914:Cells, Immobilized; D006863:Hydrogen-Ion Concentration; D011409:Propylene Glycols / Q000032:analysis / Q000378:metabolism*; D012240:Rhodococcus / Q000201:enzymology / Q000378:metabolism*; D013429:Sulfatases / Q000378:metabolism; D013431:Sulfates / Q000032:analysis / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D011409:Propylene Glycols; D013431:Sulfates; C041787:1,3-propanediol; D013429:Sulfatases