title: Isolation and characterization of novel microsatellite markers from Siamese fighting fish (Betta splendens, Osphronemidae, Anabantoidei) and their transferability to related species, B. smaragdina and B. imbellis.
abstract: Ten novel microsatellite markers were developed and characterized from Siamese fighting fish (Betta splendens). Nine of ten markers were polymorphic, exhibiting an allelic number (NA) from 2 to 6 alleles per locus. The effective number of alleles (NE) ranged from 1.60 to 3.08 (average of 2.30). The observed (HO) and expected (HE) heterozygosities ranged from 0.13 to 0.67 (average of 0.39) and 0.29 to 0.63 (average of 0.50), respectively. Linkage disequilibrium was not significantly detected for any pair of loci, and only two loci (BettaMS23 and BettaMS28) showed significant deviations from Hardy-Weinberg expectations. Of these, six loci could be amplified in genomic DNA of the closely related species B. imbellis and three loci in B. smaragdina. These microsatellite markers could be used as a tool to investigate genetic diversity and population structure, as well as breeding programs in hatcheries.
authors.affiliation: Laboratory of Animal Cytogenetics and Comparative Genomics, Department of Genetics, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: V
authors.initials: V
authors.lastname: Chailertrit
authors.affiliation: Department of Genetics, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: A
authors.initials: A
authors.lastname: Swatdipong
authors.affiliation: Department of Genetics, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Peyachoknagul
authors.affiliation: Department of Marine Science, Faculty of Fisheries, Kasetsart University, Bangkok, Thailand.
authors.forename: J
authors.initials: J
authors.lastname: Salaenoi
authors.affiliation: Laboratory of Animal Cytogenetics and Comparative Genomics, Department of Genetics, Faculty of Science, Kasetsart University, Bangkok, Thailand kornsorn.s@ku.ac.th.
authors.forename: K
authors.initials: K
authors.lastname: Srikulnath
mesh_terms: D000483:Alleles; D000818:Animals; D005399:Fishes / Q000145:classification / Q000235:genetics*; D056426:Genetic Loci; D014644:Genetic Variation; D005828:Genetics, Population; D018895:Microsatellite Repeats; D013045:Species Specificity