title: MicroRNA in situ hybridization in tissue microarrays.
abstract: In situ hybridization is used to visualize nucleic acids in microscopic tissue sections and has in recent years been used successfully to detect microRNAs. We have further optimized a technique to detect and semiquantitatively assay microRNA expression in tissue microarrays derived from formalin-fixed paraffin-embedded archival tumor tissue.
authors.affiliation: Cancer Research UK, Cambridge Institute for Julia Turnock-Jones, University of Cambridge, Cambridge, UK.
authors.forename: Julia J
authors.initials: JJ
authors.lastname: Turnock-Jones
authors.forename: John P C
authors.initials: JP
authors.lastname: Le Quesne
mesh_terms: D005557:Formaldehyde / Q000737:chemistry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D009369:Neoplasms / Q000235:genetics*; D016612:Paraffin Embedding / Q000379:methods; D046888:Tissue Array Analysis / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods
chemical_list: D035683:MicroRNAs; D005557:Formaldehyde