title: Down-regulation of BTG3 promotes cell proliferation, migration and invasion and predicts survival in gastric cancer.
abstract: BACKGROUND
  Gastric cancer (GC) is one of the most common malignancies in China. B-cell translocation gene 3 (BTG3) has been identified as a tumor suppressor in several tumors, but its role in GC remains unknown. This study aimed to detect the expression of BTG3 and its prognostic value in GC tissues and determine its function in the progression of GC.
  
  
  METHODS
  The expression of BTG3 was detected in GC cell lines and tissues by real-time RT-PCR, Western blot or immunohistochemistry. A series of in vitro and in vivo assays were performed to evaluate the effect of BTG3 on proliferation, migration and invasion of GC cells.
  
  
  RESULTS
  B-cell translocation gene 3 was obviously down-regulated in GC tissues. Its expression was positively correlated with distant metastasis (P < 0.05). Patients with lower BTG3 expression had shorter overall survival time (P = 0.015). BTG3 suppressed the proliferation of GC cells in vitro and in vivo. It also inhibited migration and invasion of GC cells in vitro.
  
  
  CONCLUSIONS
  Down-regulation of BTG3 is closely associated with proliferation, migration and invasion in GC. It may be a novel prognostic biomarker for GC patients.
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong Province, People's Republic of China.
authors.forename: X L
authors.initials: XL
authors.lastname: Ren
authors.forename: X H
authors.initials: XH
authors.lastname: Zhu
authors.forename: X M
authors.initials: XM
authors.lastname: Li
authors.forename: Y L
authors.initials: YL
authors.lastname: Li
authors.forename: J M
authors.initials: JM
authors.lastname: Wang
authors.forename: P X
authors.initials: PX
authors.lastname: Wu
authors.forename: Z B
authors.initials: ZB
authors.lastname: Lv
authors.forename: W H
authors.initials: WH
authors.lastname: Ma
authors.forename: W T
authors.initials: WT
authors.lastname: Liao
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Ding
authors.forename: L
authors.initials: L
authors.lastname: Liang
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002453:Cell Cycle; D018797:Cell Cycle Proteins; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism / Q000401:mortality* / Q000473:pathology*; D015996:Survival Rate; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: C424522:BTG3 protein, human; D018797:Cell Cycle Proteins; D011506:Proteins; D012333:RNA, Messenger