title: Comparative metabolome analysis of cultured fetal and adult hepatocytes in humans.
abstract: The liver is the central organ of metabolism, but its function varies during development from fetus to adult. In this study, we comprehensively analyzed and compared metabolites in fetal and adult hepatocytes, the major parenchymal cell in the liver, from human donors. We identified 211 metabolites (116 anions and 95 cations) by capillary electrophoresis-time-of-flight mass spectrometry (CE-TOFMS) in the hepatocytes cultured in vitro. Principal component analysis and hierarchical clustering analysis of the relative amounts of metabolites clearly classified hepatocytes into 2 groups that were consistent with their origin, i.e., the fetus and adult. The amounts of most metabolites in the glycolysis/glyconeogenesis pathway, tricarboxylic acid cycle and urea cycle were lower in fetal hepatocytes than in adult hepatocytes. These results suggest different susceptibility of the fetal and adult liver to toxic insults affecting energy metabolism.
authors.affiliation: Division of Pharmacology, National Institute of Health Sciences.
authors.forename: Su-Ryang
authors.initials: SR
authors.lastname: Kim
authors.forename: Takashi
authors.initials: T
authors.lastname: Kubo
authors.forename: Yukie
authors.initials: Y
authors.lastname: Kuroda
authors.forename: Maki
authors.initials: M
authors.lastname: Hojyo
authors.forename: Takuya
authors.initials: T
authors.lastname: Matsuo
authors.forename: Atsuko
authors.initials: A
authors.lastname: Miyajima
authors.forename: Makoto
authors.initials: M
authors.lastname: Usami
authors.forename: Yuko
authors.initials: Y
authors.lastname: Sekino
authors.forename: Taku
authors.initials: T
authors.lastname: Matsushita
authors.forename: Seiichi
authors.initials: S
authors.lastname: Ishida
mesh_terms: D002478:Cells, Cultured; D002952:Citric Acid Cycle; D016000:Cluster Analysis; D019075:Electrophoresis, Capillary; D004734:Energy Metabolism; D006019:Glycolysis; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000166:cytology / Q000196:embryology; D013058:Mass Spectrometry; D055442:Metabolome; D055432:Metabolomics; D014508:Urea / Q000378:metabolism
chemical_list: D014508:Urea