title: Shotgun assembly of the assassin bug Brontostoma colossus mitochondrial genome (Heteroptera, Reduviidae).
abstract: The complete mitochondrial genome of the assassin bug Brontostoma colossus (Distant, 1902) (Heteroptera: Reduviidae) has been sequenced using a genome-skimming approach on an Illumina Hiseq 2000 platform. Fifty-four additional heteropteran mitogenomes, including five assassin bug species, were retrieved to allow for comparisons and phylogenetic analyses. The mitochondrial genome of B. colossus was determined to be 16,625 bp long, and consists of 13 protein-coding genes (PCGs), 23 transfer-RNA genes (tRNAs), two ribosomal-RNA genes (rRNAs), and one control region. The nucleotide composition is biased toward adenine and thymine (A+T=73.4%). Overall, architecture, nucleotide composition and genome asymmetry are similar among all available assassin bug mitogenomes. All PCGs have usual start-codons (Met and Ile). Three T and two TA incomplete termination codons were identified adjacent to tRNAs, which was consistent with the punctuation model for primary transcripts processing followed by 3' polyadenylation of mature mRNA. All tRNAs exhibit the classic clover-leaf secondary structure except for tRNASer(AGN) in which the DHU arm forms a simple loop. Two notable features are present in the B. colossus mitogenome: (i) a 131 bp duplicated unit including the complete tRNAArg gene, resulting in 23 potentially functional tRNAs in total, and (ii) a 857 bp duplicated region comprising 277 bp of the srRNA gene and 580 bp of the control region. A phylogenetic analysis based on 55 true bug mitogenomes confirmed that B. colossus belongs to Reduviidae, but contradicted a widely accepted hypothesis. This highlights the limits of phylogenetic analyses based on mitochondrial data only.
authors.affiliation: Laboratoire EDB (UMR 5174, CNRS/UPS/ENFA), Université Paul Sabatier, Bât. 4R1, 118 route de Narbonne, 31062, Toulouse cedex 9, France. Electronic address: arthur.kocher@gmail.com.
authors.forename: Arthur
authors.initials: A
authors.lastname: Kocher
authors.affiliation: Laboratoire EDB (UMR 5174, CNRS/UPS/ENFA), Université Paul Sabatier, Bât. 4R1, 118 route de Narbonne, 31062, Toulouse cedex 9, France; Division of Animal Biology, Department of Biology, School of Natural Sciences, University of Patras, GR-26500 Patras, Greece. Electronic address: mariakamilari@gmail.com.
authors.forename: Maria
authors.initials: M
authors.lastname: Kamilari
authors.affiliation: INRA, GeT-PlaGe, UAR 1209 Département de génétique animale, INRA Auzeville, 31326 Castanet-Tolosan, France. Electronic address: emeline.lhuillier@udear.cnrs.fr.
authors.forename: Emeline
authors.initials: E
authors.lastname: Lhuillier
authors.affiliation: Laboratoire d'Ecologie Alpine, CNRS UMR 5553, Université Joseph Fourier, 38041 Grenoble, France. Electronic address: eric.coissac@inrialpes.fr.
authors.forename: Eric
authors.initials: E
authors.lastname: Coissac
authors.affiliation: Équipe «Écosystèmes Amazoniens et Pathologie Tropicale» (EA 3593), Faculté de Médecine Hyacinthe Bastaraud de l'Université des Antilles et de la Guyane, Campus Saint Denis, av. d'Estrées, F-97306 Cayenne, French Guiana. Electronic address: peneau.julie@wanadoo.fr.
authors.forename: Julie
authors.initials: J
authors.lastname: Péneau
authors.affiliation: Laboratoire EDB (UMR 5174, CNRS/UPS/ENFA), Université Paul Sabatier, Bât. 4R1, 118 route de Narbonne, 31062, Toulouse cedex 9, France. Electronic address: jerome.chave@univ-tlse3.fr.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Chave
authors.affiliation: Laboratoire EDB (UMR 5174, CNRS/UPS/ENFA), Université Paul Sabatier, Bât. 4R1, 118 route de Narbonne, 31062, Toulouse cedex 9, France. Electronic address: jerome.murienne@univ-tlse3.fr.
authors.forename: Jerôme
authors.initials: J
authors.lastname: Murienne
mesh_terms: D000818:Animals; D001483:Base Sequence; D018387:Codon, Initiator / Q000235:genetics; D018388:Codon, Terminator / Q000235:genetics; D049750:Genome, Insect / Q000235:genetics; D054629:Genome, Mitochondrial / Q000235:genetics*; D020062:Heteroptera / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D020064:Reduviidae / Q000235:genetics*
chemical_list: D018387:Codon, Initiator; D018388:Codon, Terminator; D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: Genome skimming; Next-generation sequencing; Phylogenomic