title: In vitro antioxidant and mineral-chelating properties of natural and autocleaved ovotransferrin.
abstract: BACKGROUND
  Egg white proteins can be excellent substrates for the development of functional foods and nutraceuticals. In this study, several in vitro antioxidant methods, namely the β-carotene linoleate model system, the ferric thiocyanate method, the 2-thiobarbituric acid-reactive substances (TBARS) method and copper/calcium ion chelation, were used to determine the antioxidant capacity of natural and autocleaved ovotransferrin.
  
  
  RESULTS
  Autocleaved ovotransferrin was prepared by reducing natural ovotransferrin with tris(2-carboxyethyl)phosphine (TCEP) for 6 h at 37 °C. Autocleaved ovotransferrin suppressed the discoloration of β-carotene effectively and prevented the oxidation of linoleic acid during 5 days of storage at 4 °C. However, the concentration of autocleaved ovotransferrin had no influence on its antioxidant effect. Similarly, the highest TBARS values were obtained from autocleaved ovotransferrin (>90%) and the lowest value in natural ovotransferrin (24%) during incubation at 37 °C for 48 h. The hydrolysates obtained from autocleaved ovotranferrin showed better copper/calcium-solublilizing activity than those from natural ovotransferrin.
  
  
  CONCLUSIONS
  The results indicated that autocleaved ovotransferrin has the potential to be used as a natural antioxidant in foods.
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Korea.|Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Moon
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Korea.
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Animal Science, Iowa State University, Ames, IA 50011, USA.|Department of Animal Science and Technology, Sunchon National University, Sunchon 540-742, Korea.
authors.forename: Dong Uk
authors.initials: DU
authors.lastname: Ahn
authors.affiliation: Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Korea.|Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea.
authors.forename: Hyun-Dong
authors.initials: HD
authors.lastname: Paik
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry*; D003207:Conalbumin / Q000737:chemistry*; D006358:Hot Temperature; D008903:Minerals / Q000737:chemistry*; D019207:beta Carotene / Q000737:chemistry
chemical_list: D000975:Antioxidants; D002614:Chelating Agents; D008903:Minerals; D019207:beta Carotene; D003207:Conalbumin
keywords: antioxidant effect; autocleaved ovotransferrin; calcium-binding assay; ovotransferrin