title: Interaction between Gallotannin and a Recombinant Form of Arginine Kinase of Trypanosoma brucei: Thermodynamic and Spectrofluorimetric Evaluation.
abstract: Current chemotherapies against trypanosomiasis are beset with diverse challenges, a situation which underscores the numerous research efforts aimed at finding newer and effective treatments. Arginine kinase of trypanosome has been validated as target for drug development against trypanosomiasis. The present study investigated the interaction between a recombinant form of the arginine kinase (rTbAK) of trypanosome and gallotannin. The interaction between gallotannin and recombinant arginine kinase of Trypanosoma brucei caused significant decrease of enzyme activity. Kinetic analysis revealed the interaction to be of noncompetitive inhibition. Further thermodynamic analysis showed that the interaction between gallotannin and the recombinant arginine kinase was nonspontaneous and involved hydrophobic forces. The K sv values and the FRET analysis suggest that static quenching of fluorescence intensity by gallotannin was static. Data revealed inhibitory interactions between gallotannin and rTbAK of trypanosome. Although the mechanism of inhibition is not clear yet, molecular docking studies are ongoing to clearly define the inhibitory interactions between the gallotannin and rTbAK. The knowledge of such binding properties would enrich development of selective inhibitors for the arginine kinase of Trypanosoma brucei.
authors.affiliation: Department of Biological Sciences, Landmark University, Omu-Aran 370102, Nigeria.
authors.forename: O S
authors.initials: OS
authors.lastname: Adeyemi
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, Ilorin 240001, Nigeria.
authors.forename: A F
authors.initials: AF
authors.lastname: Sulaiman
authors.affiliation: Department of Biochemistry, Ambrose Alli University, Ekpoma 310001, Nigeria.
authors.forename: O M
authors.initials: OM
authors.lastname: Iniaghe