title: Identification of lactobacilli with inhibitory effect on biofilm formation by pathogenic bacteria on stainless steel surfaces.
abstract: Two hundred and thirty individual clones of microorganisms were recovered from milk tanks and milking machine surfaces at two distinct farms (Bejaja City, Algeria). Of these clones, 130 were identified as lactic acid bacteria (LAB). In addition Escherichia coli, Salmonella, Staphylococcus aureus and Pseudomonas aeruginosa species were identified in the remaining 100 isolates-spoilage isolate. These isolates were assayed for ability to form biofilms. S. aureus, Lactobacillus brevis strains LB1F2, LB14F1 and LB15F1, and Lactobacillus pentosus strains LB2F2 and LB3F2 were identified as the best biofilm formers. Besides, these LAB isolates were able to produce proteinaceous substances with antagonism against the aforementioned spoilage isolates, when grown in MRS or TSB-YE media. During the screening, L. pentosus LB3F2 exhibited the highest antibacterial activity when grown in TSB-YE medium at 30 °C. Additionally, L. pentosus LB3F2 was able to strongly hamper the adhesion of S. aureus SA3 on abiotic surfaces as polystyrene and stainless steel slides. LAB isolates did not show any hemolytic activity and all of them were sensitive to different families of antibiotic tested. It should be pointed out that LB3F2 isolate was not cytotoxic on the intestinal cells but could stimulate their metabolic activity. This report unveiled the potential of LB1F2, LB14F1, LB15F1, LB2F2, and LB3F2 isolates to be used as natural barrier or competitive exclusion organism in the food processing sector as well as a positive biofilm forming bacteria.
authors.affiliation: Laboratoire de Microbiologie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia, Algeria.
authors.forename: Fatma
authors.initials: F
authors.lastname: Ait Ouali
authors.affiliation: Laboratoire Régional de Recherche en Agroalimentaire et Biotechnologies: Institut Charles Viollette, Bâtiment Polytech'Lille, Université Lille 1, Avenue Paul Langevin, Cité Scientifique, 59655 Villeneuve d'Ascq Cedex, France; Centre AZM de Biotechnologie, EDST-Université Libanaise Tripoli-Lebanon, Faculté de santé publique section 3, Université Libanaise, Tripoli, Lebanon.
authors.forename: Imad
authors.initials: I
authors.lastname: Al Kassaa
authors.affiliation: Laboratoire Régional de Recherche en Agroalimentaire et Biotechnologies: Institut Charles Viollette, Bâtiment Polytech'Lille, Université Lille 1, Avenue Paul Langevin, Cité Scientifique, 59655 Villeneuve d'Ascq Cedex, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Cudennec
authors.affiliation: Laboratoire Régional de Recherche en Agroalimentaire et Biotechnologies: Institut Charles Viollette, Bâtiment Polytech'Lille, Université Lille 1, Avenue Paul Langevin, Cité Scientifique, 59655 Villeneuve d'Ascq Cedex, France.
authors.forename: Marwan
authors.initials: M
authors.lastname: Abdallah
authors.affiliation: Laboratoire de Microbiologie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia, Algeria.
authors.forename: Farida
authors.initials: F
authors.lastname: Bendali
authors.affiliation: Laboratoire de Microbiologie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia, Algeria.
authors.forename: Djamila
authors.initials: D
authors.lastname: Sadoun
authors.affiliation: Laboratoire Régional de Recherche en Agroalimentaire et Biotechnologies: Institut Charles Viollette, Bâtiment Polytech'Lille, Université Lille 1, Avenue Paul Langevin, Cité Scientifique, 59655 Villeneuve d'Ascq Cedex, France.
authors.forename: Nour-Eddine
authors.initials: NE
authors.lastname: Chihib
authors.affiliation: Laboratoire Régional de Recherche en Agroalimentaire et Biotechnologies: Institut Charles Viollette, Bâtiment Polytech'Lille, Université Lille 1, Avenue Paul Langevin, Cité Scientifique, 59655 Villeneuve d'Ascq Cedex, France. Electronic address: djamel.drider@univ-lille1.fr.
authors.forename: Djamel
authors.initials: D
authors.lastname: Drider
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000898:Antibiosis / Q000502:physiology*; D001419:Bacteria / Q000187:drug effects; D001422:Bacterial Adhesion; D018407:Bacterial Physiological Phenomena; D018441:Biofilms; D045744:Cell Line, Tumor; D005525:Food-Processing Industry / Q000295:instrumentation / Q000592:standards; D007778:Lactobacillus / Q000502:physiology*; D051379:Mice; D011137:Polystyrenes; D013193:Stainless Steel
chemical_list: D000900:Anti-Bacterial Agents; D011137:Polystyrenes; D013193:Stainless Steel
keywords: Antagonism; Biofilm; Cytotoxicity; Lactic acid bacteria; S. aureus; Stainless steel