title: Magnetic liposomes for colorectal cancer cells therapy by high-frequency magnetic field treatment.
abstract: In this study, we developed the cancer treatment through the combination of chemotherapy and thermotherapy using doxorubicin-loaded magnetic liposomes. The citric acid-coated magnetic nanoparticles (CAMNP, ca. 10 nm) and doxorubicin were encapsulated into the liposome (HSPC/DSPE/cholesterol = 12.5:1:8.25) by rotary evaporation and ultrasonication process. The resultant magnetic liposomes (ca. 90 to 130 nm) were subject to characterization including transmission electron microscopy (TEM), dynamic light scattering (DLS), X-ray diffraction (XRD), zeta potential, Fourier transform infrared (FTIR) spectrophotometer, and fluorescence microscope. In vitro cytotoxicity of the drug carrier platform was investigated through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using L-929 cells, as the mammalian cell model. In vitro cytotoxicity and hyperthermia (inductive heating) studies were evaluated against colorectal cancer (CT-26 cells) with high-frequency magnetic field (HFMF) exposure. MTT assay revealed that these drug carriers exhibited no cytotoxicity against L-929 cells, suggesting excellent biocompatibility. When the magnetic liposomes with 1 μM doxorubicin was used to treat CT-26 cells in combination with HFMF exposure, approximately 56% cells were killed and found to be more effective than either hyperthermia or chemotherapy treatment individually. Therefore, these results show that the synergistic effects between chemotherapy (drug-controlled release) and hyperthermia increase the capability to kill cancer cells.
authors.affiliation: Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei, 106, Taiwan.
authors.forename: Andri
authors.initials: A
authors.lastname: Hardiansyah
authors.affiliation: Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei, 106, Taiwan.
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Huang
authors.affiliation: Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei, 106, Taiwan.
authors.forename: Ming-Chien
authors.initials: MC
authors.lastname: Yang
authors.affiliation: Department of Materials Engineering, Ming Chi University of Technology, New Taipei City, 24301, Taiwan.
authors.forename: Ting-Yu
authors.initials: TY
authors.lastname: Liu
authors.affiliation: Institute of Microbiology and Immunology, School of Life Science, National Yang-Ming University, Taipei, 11221, Taiwan.
authors.forename: Sung-Chen
authors.initials: SC
authors.lastname: Tsai
authors.affiliation: Institute of Microbiology and Immunology, School of Life Science, National Yang-Ming University, Taipei, 11221, Taiwan.
authors.forename: Chih-Yung
authors.initials: CY
authors.lastname: Yang
authors.affiliation: Institute of Polymer Science and Engineering, National Taiwan University, Taipei, 106, Taiwan.
authors.forename: Chih-Yu
authors.initials: CY
authors.lastname: Kuo
authors.affiliation: Department of Materials Engineering, Ming Chi University of Technology, New Taipei City, 24301, Taiwan.
authors.forename: Tzu-Yi
authors.initials: TY
authors.lastname: Chan
authors.affiliation: Department of Materials Engineering, Ming Chi University of Technology, New Taipei City, 24301, Taiwan.
authors.forename: Hui-Ming
authors.initials: HM
authors.lastname: Zou
authors.affiliation: Institute of Microbiology and Immunology, School of Life Science, National Yang-Ming University, Taipei, 11221, Taiwan.
authors.forename: Wei-Nan
authors.initials: WN
authors.lastname: Lian
authors.affiliation: Institute of Microbiology and Immunology, School of Life Science, National Yang-Ming University, Taipei, 11221, Taiwan.
authors.forename: Chi-Hung
authors.initials: CH
authors.lastname: Lin
keywords: Colorectal cancer; Drug controlled release; High-frequency magnetic field; Liposomes; Magnetic nanoparticle