title: Research on protein thermal condensation detection based on phase modulation SPR imaging.
abstract: This article presents a novel SPR imaging biomolecular interaction detection method based on time domain phase modulation. An experimental apparatus of SPR imaging biomolecular interaction detection based on TDPM is established to detect biomolecular interaction. During the experimental pretreatment process, we prepared the 2×2 lysozyme array chip and detected lysozyme thermal condensation state with the experimental apparatus. Using the Stoilov algorithm, we were able to extract the changed phase information as well as obtain the interactive SPR curves and calculate kinetic parameters. This method can sensitively acquire real-time phase change caused by biomolecular interaction based on interference imaging and resolve the related bioinformation, which is a potential tool for proteomics research.
authors.affiliation: College of Communications and Electronics Engineering, Qiqihar University, Qiqihar Heilongjiang, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: He
authors.affiliation: College of Communications and Electronics Engineering, Qiqihar University, Qiqihar Heilongjiang, People's Republic of China.
authors.forename: Zi-Yang
authors.initials: ZY
authors.lastname: Kang
authors.affiliation: College of Communications and Electronics Engineering, Qiqihar University, Qiqihar Heilongjiang, People's Republic of China.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Wan
authors.affiliation: College of Communications and Electronics Engineering, Qiqihar University, Qiqihar Heilongjiang, People's Republic of China.
authors.forename: Han-Xiong
authors.initials: HX
authors.lastname: Fang
mesh_terms: D000465:Algorithms; D000818:Animals; D004867:Equipment Design; D006801:Humans; D009113:Muramidase / Q000737:chemistry*; D011487:Protein Conformation; D011489:Protein Denaturation; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods; D013696:Temperature
chemical_list: D009113:Muramidase
keywords: biomolecular interaction; lysozyme thermal condensation; surface plasmon resonance (SPR); time domain phase modulation (TDPM)