title: Effect of intracerebral transplantation of mesenchymal stem cells on reactivity of pial arterioles in old rats.
abstract: Using a TV device for studying microcirculation (×160), we analyzed the responses of arterioles in the pia mater of the sensorimotor cortex in young (2-3 months) and old (22-24 months) rats after local application of a vasoconstrictor (norepinephrine, 10(-6) M) or vasodilator (acetylcholine, 10(-6) M). The responses of the arterioles were evaluated by changes in their diameter and by the number of responding vessels in the field of view. The constrictor responses of the pial arteries to norepinephrine did not significantly differ in intact young and old rats. The number and degree of dilatory responses to acetylcholine in old rats were lower than in young animals by 14 and 30%, respectively. Intracerebral transplantation of mesenchymal stem cells to old rats had practically no effect on reactivity of pial arterioles to acetylcholine, while the number of constricted vessels in response to norepinephrine increased by ~20%.
authors.affiliation: Laboratory of Physiology of Circulation, I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia, sib@kolt.infran.ru.
authors.forename: I B
authors.initials: IB
authors.lastname: Sokolova
authors.forename: I V
authors.initials: IV
authors.lastname: Sergeev
authors.forename: S V
authors.initials: SV
authors.lastname: Anisimov
authors.forename: M V
authors.initials: MV
authors.lastname: Puzanov
authors.forename: D P
authors.initials: DP
authors.lastname: Dvoretskii
mesh_terms: D000818:Animals; D001160:Arterioles / Q000502:physiology*; D001921:Brain / Q000098:blood supply / Q000502:physiology*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D051381:Rats; D011921:Rats, Inbred WKY