title: Promoting the selection and maintenance of fetal liver stem/progenitor cell colonies by layer-by-layer polypeptide tethered supported lipid bilayer.
abstract: In this study, we designed and constructed a series of layer-by-layer polypeptide adsorbed supported lipid bilayer (SLB) films as a novel and label-free platform for the isolation and maintenance of rare populated stem cells. In particular, four alternative layers of anionic poly-l-glutamic acid and cationic poly-l-lysine were sequentially deposited on an anionic SLB. We found that the fetal liver stem/progenitor cells from the primary culture were selected and formed colonies on all layer-by-layer polypeptide adsorbed SLB surfaces, regardless of the number of alternative layers and the net charges on those layers. Interestingly, these isolated stem/progenitor cells formed colonies which were maintained for an 8 day observation period. Quartz crystal microbalance with dissipation measurements showed that all SLB-polypeptide films were protein resistant with serum levels significantly lower than those on the polypeptide multilayer films without an underlying SLB. We suggest the fluidic SLB promotes selective binding while minimizing the cell-surface interaction due to its nonfouling nature, thus limiting stem cell colonies from spreading.
authors.affiliation: Graduate Institute of Biochemical and Biomedical Engineering, Chang-Gung University , Tao-yuan 333, Taiwan, R.O.C.
authors.forename: I-Chi
authors.initials: IC
authors.lastname: Lee
authors.forename: Yung-Chiang
authors.initials: YC
authors.lastname: Liu
authors.forename: Hsuan-Ang
authors.initials: HA
authors.lastname: Tsai
authors.forename: Chia-Ning
authors.initials: CN
authors.lastname: Shen
authors.forename: Ying-Chih
authors.initials: YC
authors.lastname: Chang
mesh_terms: D005333:Fetus / Q000166:cytology / Q000196:embryology; D018698:Glutamic Acid / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000196:embryology; D008239:Lysine / Q000737:chemistry; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*; D013499:Surface Properties
chemical_list: D008051:Lipid Bilayers; D010455:Peptides; D018698:Glutamic Acid; D008239:Lysine
keywords: fetal liver stem/progenitor cells; label free; layer-by-layer polypeptide films; rare cell isolation; stem cell maintenance; supported lipid bilayer (SLB)