title: Elevated expression level of long noncoding RNA MALAT-1 facilitates cell growth, migration and invasion in pancreatic cancer.
abstract: Pancreatic cancer is one of the most aggressive solid malignancies with a dismal survival rate. Recent studies have shown that high expression levels of long noncoding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript-1 (MALAT-1) correlate with several solid tumors. However, the underlying molecular mechanisms and its clinical significance in pancreatic cancer remain to be elucidated. In the present study, our results showed that MALAT-1 expression levels were upregulated in pancreatic cancer tissues compared with adjacent noncancerous controls. Consistently, higher expression level of MALAT-1 was found in all seven pancreatic cancer cell lines relative to the human pancreatic ductal epithelial cell. Further function analysis revealed that downregulation of MALAT-1 could inhibit tumor cell proliferation and decrease cell migration and invasion in vitro. The underlying mechanisms are possibly involved in inducing G2/M cell cycle arrest, promoting cell apoptosis, suppressing epithelial-mesenchymal transition and reducing cancer stem-like properties. In conclusion, this study indicated that MALAT-1 may serve as an oncogenic lncRNA that is involved in malignancy phenotypes of pancreatic cancer. Therefore, it may be used as a potential therapeutic target.
authors.affiliation: Department of Oncology and Shanghai Key Laboratory of Pancreatic Diseases, First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 201620, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Jiao
authors.affiliation: Department of Oncology and Shanghai Key Laboratory of Pancreatic Diseases, First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 201620, P.R. China.
authors.forename: Hai
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Pathology, First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 201620, P.R. China.
authors.forename: Cuncun
authors.initials: C
authors.lastname: Yuan
authors.affiliation: Department of Oncology and Shanghai Key Laboratory of Pancreatic Diseases, First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 201620, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Oncology and Shanghai Key Laboratory of Pancreatic Diseases, First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 201620, P.R. China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Department of Oncology and Shanghai Key Laboratory of Pancreatic Diseases, First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 201620, P.R. China.
authors.forename: Ziliang
authors.initials: Z
authors.lastname: Jin
authors.affiliation: Department of Oncology and Shanghai Key Laboratory of Pancreatic Diseases, First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 201620, P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Department of Oncology and Shanghai Key Laboratory of Pancreatic Diseases, First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 201620, P.R. China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition; D059565:G2 Phase Cell Cycle Checkpoints; D015870:Gene Expression; D006801:Humans; D009361:Neoplasm Invasiveness; D014411:Neoplastic Stem Cells / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C557041:MALAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding