title: The effects of harvesting media on biological characteristics and repair potential of neural stem cells after traumatic brain injury.
abstract: Various solutions are utilized widely for the isolation, harvesting, sorting, testing and transplantation of neural stem cells (NSCs), whereas the effects of harvesting media on the biological characteristics and repair potential of NSCs remain unclear. To examine some of these effects, NSCs were isolated from cortex of E14.5 mice and exposed to the conventional harvesting media [0.9% saline (Saline), phosphate-buffered saline (PBS) or artificial cerebrospinal fluid (ACSF)] or the proliferation culture medium (PCM) for different durations at 4°C. Treated NSCs were grafted by in situ injection into the lesion sites of traumatic brain injury (TBI) mice. In vitro, harvesting media-exposed NSCs displayed time-dependent reduction of viability and proliferation. S phase entry decreased in harvesting media-exposed cells, which was associated with upregulation of p53 protein and downregulation of cyclin E1 protein. Moreover, harvesting media exposure induced the necrosis and apoptosis of NSCs. The levels of Fas-L, cleaved caspase 3 and 8 were increased, which suggests that the death receptor signaling pathway is involved in the apoptosis of NSCs. In addition, exposure to Saline did not facilitate the neuronal differentiation of NSCs, suggesting that Saline exposure may be disadvantageous for neurogenesis. In vivo, NSC-mediated functional recovery in harvesting media-exposed NSC groups was notably attenuated in comparison with the PCM-exposed NSC group. In conclusion, harvesting media exposure modulates the biological characteristics and repair potential of NSCs after TBI. Our results suggest that insight of the effects of harvesting media exposure on NSCs is critical for developing strategies to assure the successful long-term engraftment of NSCs.
authors.affiliation: Department of Anatomy, School of Basic Medical Sciences, Harbin Medical University, Harbin, Heilongjiang Province, China.
authors.forename: Shengliang
authors.initials: S
authors.lastname: Liu
authors.affiliation: The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China.
authors.forename: Zhuying
authors.initials: Z
authors.lastname: Li
authors.affiliation: The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Fu
authors.affiliation: Department of Anatomy, School of Basic Medical Sciences, Harbin Medical University, Harbin, Heilongjiang Province, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Anatomy, School of Basic Medical Sciences, Harbin Medical University, Harbin, Heilongjiang Province, China.
authors.forename: Fengyan
authors.initials: F
authors.lastname: Xu
authors.affiliation: Prefectural University of Hiroshima, Hiroshima, Japan.
authors.forename: Toshihide
authors.initials: T
authors.lastname: Harada
authors.affiliation: The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Lou
authors.affiliation: The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chu
authors.affiliation: Department of Anatomy, School of Basic Medical Sciences, Harbin Medical University, Harbin, Heilongjiang Province, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Sun
authors.affiliation: The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Xu
authors.affiliation: The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Anatomy, School of Basic Medical Sciences, Harbin Medical University, Harbin, Heilongjiang Province, China.
authors.forename: Lianhong
authors.initials: L
authors.lastname: Jin
authors.affiliation: The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Department of Anatomy, School of Basic Medical Sciences, Harbin Medical University, Harbin, Heilongjiang Province, China.
authors.forename: Shuliang
authors.initials: S
authors.lastname: Wu
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000628:therapy*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002540:Cerebral Cortex / Q000166:cytology; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D019927:Cyclin E / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D058953:Neural Stem Cells / Q000187:drug effects* / Q000637:transplantation; D033581:Stem Cell Transplantation; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D003470:Culture Media; D019927:Cyclin E; D016159:Tumor Suppressor Protein p53