title: Histological effects and localization of dissolved microcystins LR and LW in the mayfly Ecdyonurus angelieri Thomas (Insecta, Ephemeroptera).
abstract: The ability of microcystins (MCs), the main group of cyanotoxins, to affect the physiological processes and tissues of insects has received little attention. Fresh water dissolved MCs represent one of the main sources of cyanotoxins. In the experiment described herein, captured wild mayfly Ecdyonurus angelieri Thomas, 1968 larvae were exposed to 5 ppb of two distinct microcystins, MC-LR and MC-LW, in separate assays. Evidence of induced mortality, MCs bioaccumulation and severe histological damage affecting fat body and alterations in the tracheal system were evident. Our results reveal the acute sensitivity of the mayfly E. angelieri to MCS, which may serve as early indicators or cyanotoxins production and the quality of freshwater streams.
authors.affiliation: Department of Cell Biology and Histology, University of Murcia, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Liarte
authors.affiliation: Department of Zoology and Physical Anthropology, University of Murcia, Spain. Electronic address: nubero@um.es.
authors.forename: Nicolás
authors.initials: N
authors.lastname: Ubero-Pascal
authors.affiliation: Department of Cell Biology and Histology, University of Murcia, Spain.
authors.forename: Alfonsa
authors.initials: A
authors.lastname: García-Ayala
authors.affiliation: Integrative Freshwater Ecology Group, Centro de Estudios Avanzados de Blanes, Consejo Superior de Investigaciones Científicas, Spain.
authors.forename: María-Ángeles
authors.initials: MÁ
authors.lastname: Puig
mesh_terms: D000818:Animals; D000458:Cyanobacteria / Q000737:chemistry*; D004784:Environmental Monitoring / Q000379:methods*; D065133:Ephemeroptera / Q000737:chemistry*; D004848:Epithelium / Q000187:drug effects*; D005216:Fat Body / Q000187:drug effects*; D005618:Fresh Water; D017952:Ganglia, Invertebrate / Q000378:metabolism; D007150:Immunohistochemistry; D007814:Larva / Q000737:chemistry; D008387:Marine Toxins; D052998:Microcystins / Q000633:toxicity*
chemical_list: D008387:Marine Toxins; D052998:Microcystins; C057862:cyanoginosin LR
keywords: Macroinvertebrate; Mayfly; Microcystin; Stream; Toxicity