title: Collateral Vessel Formation Causes Clinical Recovery From Limb Ischemia in a Mouse Model.
abstract: Experimental models of recovery from limb ischemia are required for evaluating novel means of treating ischemia. We describe a mouse model to assess gait after inducing limb ischemia. Gait analysis was performed using a Plexiglass tube, the floor of which contained load cells. Gait was measured in 20 mice; 10 underwent ligation of the right hind limb artery and the other 10 underwent a sham operation. The gait of the animals was measured at 1, 2, and 4 weeks following the procedure. In sham-operated animals, the gait showed no measurable change. In the ligated animals, the ratio of the right fore-to-hind limb changed from 1.07 at baseline to 1.4 at day 0 (P = .001), 1.16 (P = .012 compared with control), and 1.04 (P = .37 compared with control) at weeks 2 and 4, respectively. Gait returned to normal within 4 weeks of induction of ischemia. This model may be helpful in testing potential novel therapies.
authors.affiliation: Sheffield Vascular Institute, Northern General Hospital, Sheffield, United Kingdom Ed.Mulkern@sth.nhs.uk.
authors.forename: Edward M
authors.initials: EM
authors.lastname: Mulkern
authors.affiliation: Sheffield Vascular Institute, Northern General Hospital, Sheffield, United Kingdom.
authors.forename: Kosmas I
authors.initials: KI
authors.lastname: Paraskevas
authors.affiliation: Sheffield Vascular Institute, Northern General Hospital, Sheffield, United Kingdom.
authors.forename: Philip
authors.initials: P
authors.lastname: Chan
mesh_terms: D000818:Animals; D003097:Collateral Circulation; D004195:Disease Models, Animal; D005684:Gait; D006614:Hindlimb; D007511:Ischemia / Q000503:physiopathology*; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000098:blood supply*; D020127:Recovery of Function; D012815:Signal Processing, Computer-Assisted; D013997:Time Factors; D014160:Transducers, Pressure
keywords: angiography; animal model; collateral arteriogenesis; gait analysis; limb ischemia