title: [Clinical significance of TET2 gene expression in 157 adult acute myeloid leukemia patients with normal cytogenetics].
abstract: OBJECTIVE
  To explore the clinical significance of ten-eleven-translocation methylcytosine dioxygenase 2 (TET2) mRNA expression levels in adult acute myeloid leukemia patients with normal cytogenetics (CN-AML).
  
  
  METHODS
  Expression levels of TET2 mRNA were measured by real-time PCR in 157 adult CN-AML, and its clinical impact in CN-AML was evaluated as well.
  
  
  RESULTS
  TET2 gene expression levels from bone marrow mononuclear cells (BMMNCs) [7.29(3.41-9.99)] and CD34+ cells [6.02(5.64-6.54)] in CN-AML were significantly lower than those [BMMNCs: 8.13(6.68-9.04), P=0.026; CD34+ cells: 6.48(5.97-7.12), P=0.034] in healthy control. And TET2 mRNA level at diagnosis [7.32(6.11-8.41)] was obviously lower than that at complete remission [8.39(7.76-8.79), P<0.01]. CN-AML patients with lower levels of TET2 mRNA showed worse survival rate [(32.7±5.9)%] at 18-month than those with higher levels [(48.6±6.9)%, P=0.041]. In multivariate analysis, lower level of TET2 mRNA was an independent prognostic factor for OS [hazard ratio(HR)2.032, 95% confidence interval (CI)1.272-3.247, P=0.003] and event-free survival [HR 1.532, 95% CI 1.014-2.314, P=0.043].
  
  
  CONCLUSIONS
  The level of TET2 mRNA is significantly lower in patients with CN-AML and it is an independent negative prognostic factor. TET2 could be an important factor for the molecular-based risk stratification in CN-AML.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Zhijuan
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Mengxia
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Zhimei
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Jiyu
authors.initials: J
authors.lastname: Lou
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Tong
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Qian
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Meng
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Jin
mesh_terms: D000328:Adult; D020732:Cytogenetic Analysis; D003582:Cytogenetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D049308:Dioxygenases; D018572:Disease-Free Survival; D015870:Gene Expression; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D049308:Dioxygenases; C541081:TET2 protein, human