title: Membrane and soluble Toll-like receptor 2 in patients with psoriasis treated by Goeckerman therapy.
abstract: BACKGROUND
  Toll-like receptor (TLR) 2 belongs to the large TLR receptor family comprised of at least 10 members with different roles in innate immunity. Psoriasis is recognized as a T-cell driven immune-mediated systemic inflammatory disease with a skin manifestation. An effective therapeutic approach to treat psoriasis is Goeckerman therapy (GT). The aim of this study was to assess both the kinetics of the expression of TLR2 on blood cells and the concentration of soluble (s)TLR2 in serum of patients with psoriasis and to examine the effect of GT on both TLR2 expression and sTLR2 level.
  
  
  METHODS
  Both membrane and sTLR2 were determined in 20 patients and 20 healthy controls. sTLR2 was evaluated by enzyme-linked immunosorbent assay. Flow cytometry method was used to determine the expression of membrane TLR2 of monocytes and granulocytes.
  
  
  RESULTS
  The serum level of sTLR2 was significantly lower (P < 0.0001) in patients both before and after GT compared to the control group. Compared to the membrane expression of TLR2 on monocytes of healthy blood donors, TLR2 expression was significantly higher in patients both before and after GT (P = 0.0001). Similarly, TLR2 expression on granulocytes was significantly higher in patients both before (P = 0.0061) and after (P < 0.0001) therapy than in control.
  
  
  CONCLUSIONS
  Membrane and soluble TLR2 may be involved in the pathogenesis of psoriasis. Both remained unchanged by GT.
authors.affiliation: Department of Clinical Immunology and Allergology, University Hospital Hradec Kralove, Charles University in Prague, Hradec Kralove, Czech Republic.
authors.forename: Katerina
authors.initials: K
authors.lastname: Kondelkova
authors.forename: Jan
authors.initials: J
authors.lastname: Krejsek
authors.forename: Lenka
authors.initials: L
authors.lastname: Borska
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Fiala
authors.forename: Kveta
authors.initials: K
authors.lastname: Hamakova
authors.forename: Karel
authors.initials: K
authors.lastname: Ettler
authors.forename: Ctirad
authors.initials: C
authors.lastname: Andrys
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002462:Cell Membrane / Q000737:chemistry; D003033:Coal Tar / Q000627:therapeutic use*; D005260:Female; D006098:Granulocytes / Q000737:chemistry; D064368:Healthy Volunteers; D006801:Humans; D007641:Keratolytic Agents / Q000627:therapeutic use*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000737:chemistry; D010778:Photochemotherapy; D011565:Psoriasis / Q000097:blood* / Q000188:drug therapy*; D051195:Toll-Like Receptor 2 / Q000032:analysis* / Q000097:blood*; D014467:Ultraviolet Therapy; D055815:Young Adult
chemical_list: D007641:Keratolytic Agents; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2; D003033:Coal Tar