title: Modulation of oligodendrocyte generation during a critical temporal window after NG2 cell division.
abstract: Oligodendrocytes in the mammalian brain are continuously generated from NG2 cells throughout postnatal life. However, it is unclear when the decision is made for NG2 cells to self-renew or differentiate into oligodendrocytes after cell division. Using a combination of in vivo and ex vivo imaging and fate analysis of proliferated NG2 cells in fixed tissue, we demonstrate that in the postnatal developing mouse brain, the majority of divided NG2 cells differentiate into oligodendrocytes during a critical age-specific temporal window of 3-8 d. Notably, within this time period, damage to myelin and oligodendrocytes accelerated oligodendrocyte differentiation from divided cells, and whisker removal decreased the survival of divided cells in the deprived somatosensory cortex. These findings indicate that during the critical temporal window of plasticity, the fate of divided NG2 cells is sensitive to modulation by external signals.
authors.affiliation: 1] Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, USA. [2] Department of Neurology, Yale University School of Medicine, New Haven, Connecticut, USA.
authors.forename: Robert A
authors.identifier: 0000000160808712
authors.initials: RA
authors.lastname: Hill
authors.affiliation: Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, USA.
authors.forename: Kiran D
authors.initials: KD
authors.lastname: Patel
authors.affiliation: Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, USA.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Goncalves
authors.affiliation: Department of Neurology, Yale University School of Medicine, New Haven, Connecticut, USA.
authors.forename: Jaime
authors.identifier: 000000025000243X
authors.initials: J
authors.lastname: Grutzendler
authors.affiliation: 1] Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, USA. [2] Connecticut Stem Cell Institute, University of Connecticut, Farmington, Connecticut, USA.
authors.forename: Akiko
authors.initials: A
authors.lastname: Nishiyama
mesh_terms: D000818:Animals; D000941:Antigens / Q000378:metabolism; D001253:Astrocytes / Q000378:metabolism; D001921:Brain / Q000166:cytology; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002455:Cell Division; D019070:Cell Lineage / Q000235:genetics / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology; D005260:Female; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D055495:Neurogenesis / Q000502:physiology; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology* / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D013997:Time Factors; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D011509:Proteoglycans; D000099216:Chondroitin Sulfate Proteoglycan 4