title: Sevoflurane prevents liver inflammatory response induced by lung ischemia-reperfusion.
abstract: BACKGROUND
  Transplants cause ischemia-reperfusion (IR) injury that can affect distant organs. Liver is particularly sensitive to IR injury. The present randomized experimental study was designed to investigate a possible protective effect of sevoflurane against liver inflammatory response to lung IR in a lung upper lobe left autotransplant model.
  
  
  METHODS
  Two groups (sevoflurane and control) of eight swines each were submitted to upper lobe left lung autotransplant. Hypnotic maintenance was performed with sevoflurane 3% or propofol 8 to 10 mg/kg per hr until pneumonectomy was done; then propofol was used for all animals. Blood and liver samples were taken in four different moments: prepneumonectomy, prereperfusion, 10 min postreperfusion and 30 min postreperfusion to measure levels of interleukin (IL)-1β, IL-10, tumor necrosis factor (TNF)-α, monocyte chemotactic protein (MCP)-1, nuclear factor (NF)-κB, C-reactive protein, ferritin and caspase 3. Non-parametric test was used to find statistical meaning.
  
  
  RESULTS
  Lung IR markedly increased the expression of TNF-α, IL-1β, MCP-1, NF-κB and caspase activity in control livers compared with basal levels, whereas liver IL-10 expression decreased 10 and 30 min post-reperfusion. Sevoflurane significantly decreased TNF-α, IL-1β, MCP-1, NF-κB liver expression and caspase 3 activity. Sevoflurane also reverted the lung IR-induced decrease in IL-10 expression.
  
  
  CONCLUSIONS
  The present results indicate that lung IR caused hepatic injury. Sevoflurane attenuated liver injury in a model of upper lobe left lung autotransplant in pigs.
authors.affiliation: 1 Department of Biochemistry and Molecular Biology III, Faculty of Medicine, Complutense University of Madrid, Madrid, Spain. 2 Department of Thoracic Surgery, Gregorio Marañón University General Hospital, Madrid, Spain. 3 Department of Anaesthesiology, Gregorio Marañón University General Hospital, Madrid, Spain. 4 Department of Physiology, Faculty of Medicine, Complutense University, Madrid, Spain. 5 Address correspondence to: Lisa Rancan, D.V.M., Department of Biochemistry and Molecular Biology III, Faculty of Medicine, Universidad Complutense de Madrid Avenida Complutense s/n 28040, Madrid, Spain.
authors.forename: Lisa
authors.initials: L
authors.lastname: Rancan
authors.forename: Luis
authors.initials: L
authors.lastname: Huerta
authors.forename: Gabriel
authors.initials: G
authors.lastname: Cusati
authors.forename: Iñaki
authors.initials: I
authors.lastname: Erquicia
authors.forename: Jesús
authors.initials: J
authors.lastname: Isea
authors.forename: Sergio D
authors.initials: SD
authors.lastname: Paredes
authors.forename: Cruz
authors.initials: C
authors.lastname: García
authors.forename: Ignacio
authors.initials: I
authors.lastname: Garutti
authors.forename: Carlos
authors.initials: C
authors.lastname: Simón
authors.forename: Elena
authors.initials: E
authors.lastname: Vara
mesh_terms: D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D002097:C-Reactive Protein / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D018932:Chemokine CCL2 / Q000378:metabolism; D005293:Ferritins / Q000378:metabolism; D006439:Hemodynamics; D007249:Inflammation; D016753:Interleukin-10 / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D008168:Lung / Q000473:pathology*; D008738:Methyl Ethers / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism; D011013:Pneumonectomy; D015742:Propofol / Q000494:pharmacology; D011897:Random Allocation; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D000077149:Sevoflurane; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D018685:Anesthetics, Inhalation; D018932:Chemokine CCL2; D053583:Interleukin-1beta; D008738:Methyl Ethers; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D000077149:Sevoflurane; D002097:C-Reactive Protein; D005293:Ferritins; D053148:Caspase 3; D015742:Propofol