title: Electro-optical study of the exposure of Azospirillum brasilense carbohydrate epitopes.
abstract: The exposure of Azospirillum brasilense carbohydrate epitopes was investigated by electro-optical analysis of bacterial cell suspensions. To study changes in the electro-optical (EO) properties of the suspensions, we used antibodies generated to the complete lipopolysaccharide of A. brasilense type strain Sp7 and also antibodies to the smooth and rough O polysaccharides of Sp7. After 18 hr of culture growth, the EO signal of the suspension treated with antibodies to smooth O polysaccharide was approximately 20% lower than that of the suspension treated with antibodies to complete lipopolysaccharide (control). After 72 hr of culture growth, the strongest EO signal was observed for the cells treated with antibodies to rough O polysaccharide (approximately 46% greater than the control), whereas for the cells treated with antibodies to smooth O polysaccharide, it was much lower (approximately 23% of the control). These data were confirmed by electron microscopy. The results of the study may have importance for the rapid evaluation of changes in lipopolysaccharide form in microbial biotechnology, when the antigenic composition of the bacterial surface requires close control.
authors.affiliation: a Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences (IBPPM RAS) , Saratov , Russian Federation.
authors.forename: Olga I
authors.initials: OI
authors.lastname: Guliy
authors.forename: Larisa Yu
authors.initials: LY
authors.lastname: Matora
authors.forename: Lev A
authors.initials: LA
authors.lastname: Dykman
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Staroverov
authors.forename: Gennady L
authors.initials: GL
authors.lastname: Burygin
authors.forename: Viktor D
authors.initials: VD
authors.lastname: Bunin
authors.forename: Andrei M
authors.initials: AM
authors.lastname: Burov
authors.forename: Oleg V
authors.initials: OV
authors.lastname: Ignatov
mesh_terms: D000907:Antibodies, Bacterial / Q000494:pharmacology*; D000942:Antigens, Bacterial / Q000276:immunology; D015806:Azospirillum brasilense / Q000276:immunology* / Q000502:physiology* / Q000648:ultrastructure; D055724:Electrophysiological Phenomena; D000939:Epitopes / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D008854:Microscopy, Electron
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides
keywords: Azospirillum brasilense; antibodies; antigen carbohydrate epitopes; determination of microorganisms; electrophysical properties