title: Multiplex detection of lung cancer cells at the single-molecule level.
abstract: We develop a simple and sensitive method for multiplex detection of lung cancer cells at the single-molecule level, with a detection limit of 15 cells per mL for A549 cells and 4 cells per mL for H23 cells, without the involvement of any sequence-based amplification. This method holds great potential for further application in early clinical diagnosis, especially for the detection of rare tumor cells.
authors.affiliation: Single-molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Guangdong 518055, China. zhangcy@siat.ac.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: Hu
authors.forename: Chun-yang
authors.initials: CY
authors.lastname: Zhang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D045744:Cell Line, Tumor; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics; D061986:MCF-7 Cells; D018613:Microscopy, Confocal; D009360:Neoplastic Cells, Circulating / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism; D013388:Succinimides / Q000737:chemistry; D013843:Thiazines / Q000737:chemistry*
chemical_list: C492694:Alexa Fluor 488 carboxylic acid succinimidyl ester; D052157:Aptamers, Nucleotide; D011956:Receptors, Cell Surface; D013388:Succinimides; D013843:Thiazines; C053505:fluorescent dye D 282