title: [MiR432* regulate the replication of coxsackievirus A16 in rhabdomyosarcoma cells].
abstract: OBJECTIVE
  MicroRNAs (miRNAs) play an important role in infection and replication of virus in host cells. In this study, we examined miRNAs' effects on the replication of Coxsackievirus A16 (CA16) in rhabdomyosarcoma cells.
  
  
  METHODS
  We constructed target gene of miRNAs screening system. We used 3'untranslated region (UTR) dual luciferase reporter analysis to identify putative miRNA targets in the CA16 virus genome. First, 12 segments of CA16 virus genome were inserted to the pMIR vector and the luciferase expression were assayed to identify the target gene of putative miRNA. The reporter gene expression of the cells transfected with the vector containing 5'-UTR was significantly downregulated. Then, using online analysis programs we screened the miRNAs that may target to 5'-UTR. Furthermore, Western blot and real-time PCR test were used to study the effect of miRNAs on viral replication.
  
  
  RESULTS
  The study showed that miR432 * could stimulate the replication of CA16 virus. On the contrary, miR432 * inhibitor could suppress CA16 virus replication.
  
  
  CONCLUSIONS
  Cellular miRNAs could regulate the replication of CA16 virus in host cells. Our findings support the notion that the cellular miRNAs play an important role in the host and virus infection.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Yang
authors.forename: Po
authors.initials: P
authors.lastname: Tien
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D045744:Cell Line, Tumor; D029821:Enterovirus A, Human / Q000235:genetics / Q000502:physiology*; D004769:Enterovirus Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D015967:Gene Expression Regulation, Viral; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012208:Rhabdomyosarcoma / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D014779:Virus Replication
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; C582553:MIRN432 microRNA, human; D035683:MicroRNAs