title: [Different stimulant intensity of abdominal operation on the expression of tumor necrosis factor α and surfactant protein-A in lung of the rats].
abstract: OBJECTIVE
  To determine the influence of different stimulation of abdominal operation on lung.
  
  
  METHODS
  We randomly divided 100 SD male rats into 5 groups(n=20): An anesthesia control group (Group A), an abdominal skin 2 cm incision group (Group B), an abdominal skin+ muscle 2 cm incision group (Group C ), an abdominal skin+muscle+peritoneum 2 cm incision group (Group D), and an abdominal skin+muscle+peritoneum 2 cm incision+2 min laparoscopy in abdomen+pull exploration bowel group (Group E). Propofol was used for induction of anaesthesia by rat tail vein with 10 mg/kg, with 35-45 mg/(kg.h) during the operation. Anesthesia depth was maintained during moderate sedation without obvious analgesia action level. According to the postoperative specimens at different time, each group was divided into 4 sub groups, including 6-hour group, 1-day group, 3-day group, and 7-day group(n=5). Immunohistochemical method was used to examine tumor necrosis factor (TNF-α) and lung tissue lung surface active substances related proteins A (SP-A) of rats at different points after surgery.
  
  
  RESULTS
  There was no significant differences in the expression of TNF-α at 6 hours, 3 days, and 7 days after surgery in the 5 groups (P>0.05). The expression of TNF-α in Group D, and E was significantly higher than that in Group A at 1 day after surgery (P<0.05). Although the expression of TNF-α was Group E > Group D > Group C > Group B at 1 day, 3 days after surgery, there was no significant difference among the 4 groups. There was no significant differences in the expression of SP-A at 6 hours, 3 days, and 7 days after surgery in the 5 groups (P>0.05). The expression of SP-A in Group D and E was significantly higher than that in Group A at 1 day after surgery (P<0.05). There was no significant difference between Group D and E (P>0.05).
  
  
  CONCLUSIONS
  The greater the stimulant intensity of abdominal operation on rats, the more impact on postoperative lung. The postoperative pulmonary effect of intraperitoneal operation is greater than non-intraperitoneal operation, but in the compensation.
authors.affiliation: Department of Anesthesiology, Zhuzhou Central Hospital, Zhuzhou Hunan 412007,China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Hongwei
authors.initials: H
authors.lastname: Cai
mesh_terms: D000758:Anesthesia; D000818:Animals; D013505:Digestive System Surgical Procedures; D010535:Laparoscopy; D008168:Lung / Q000378:metabolism*; D008297:Male; D011184:Postoperative Period; D015742:Propofol; D037662:Pulmonary Surfactant-Associated Protein A / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013501:Surface-Active Agents; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D037662:Pulmonary Surfactant-Associated Protein A; D013501:Surface-Active Agents; D014409:Tumor Necrosis Factor-alpha; D015742:Propofol