title: A peptide probe for the detection of neurokinin-1 receptor by disaggregation enhanced fluorescence and magnetic resonance signals.
abstract: We report a novel peptide probe for the detection of neurokinin-1 receptor using disaggregation-caused signal enhancement. The probe was obtained via the aggregation of a modified substance P in a terpyridine-Fe (II) complex with Gd (III)-DOTA into well-defined nanostructures, which effectively weaken ligand fluorescence and slow the exchange rate of inner-sphere water molecules. This probe disaggregates upon binding to the neurokinin-1 receptor and activates the contrast agents to generate a fluorescent signal that positively enhances magnetic resonance imaging contrast and allows for the detection of overexpressed receptors on tumor cells and the identification of lung cancer using serum samples.
authors.affiliation: 1] Key Laboratory for Advanced Materials and Institute of Fine Chemicals Institution, East China University of Science and Technology, Meilong Road 130, Shanghai 200237, China [2].
authors.forename: Jingxian
authors.initials: J
authors.lastname: Wu
authors.affiliation: 1] Key Laboratory for Advanced Materials and Institute of Fine Chemicals Institution, East China University of Science and Technology, Meilong Road 130, Shanghai 200237, China [2].
authors.forename: Rongfeng
authors.initials: R
authors.lastname: Zou
authors.affiliation: College of Public Health, Nantong University, Seyuan Road 9, Nantong, Jiangsu 226019, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Clinical laboratory, Nantong Tumor Hospital, West Qingnian Road 48, Nantong, Jiangsu 226006, China.
authors.forename: Yajing
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Cancer Institutes, Fudan University Shanghai Cancer Center, Dongan Road 270, Shanghai 200032, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
authors.affiliation: Department of chemistry, Shanghai Normal University, Guilin Road 100, Shanghai 200234, China.
authors.forename: Shiping
authors.initials: S
authors.lastname: Yang
authors.affiliation: Key Laboratory for Advanced Materials and Institute of Fine Chemicals Institution, East China University of Science and Technology, Meilong Road 130, Shanghai 200237, China.
authors.forename: Junchen
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Laboratory for Advanced Materials and Institute of Fine Chemicals Institution, East China University of Science and Technology, Meilong Road 130, Shanghai 200237, China.
authors.forename: He
authors.initials: H
authors.lastname: Tian
mesh_terms: D015415:Biomarkers / Q000032:analysis; D049109:Cell Proliferation; D003287:Contrast Media; D005434:Flow Cytometry; D005453:Fluorescence; D005682:Gadolinium; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D010446:Peptide Fragments; D018040:Receptors, Neurokinin-1 / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D015415:Biomarkers; D003287:Contrast Media; D010446:Peptide Fragments; D018040:Receptors, Neurokinin-1; D005682:Gadolinium