title: [Simultaneous determination of twenty psychoactive drugs in pork by solid phase extraction coupled with ultra performance liquid chromatography-tandem mass spectrometry].
abstract: A method for the simultaneous determination of 20 psychoactive drugs in pork by solid phase extraction coupled with ultra performance liquid chromatography with electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) was established. The samples were extracted with alkalified acetonitrile, and then cleaned up using solid phase extraction with an Oasis MCX column. The UPLC separation was performed on a C18 column (50 mm x 2.1 mm, 1.7 microm) using a gradient elution with the mobile phases of 0.1% (v/v) formic acid in water and acetonitrile. The ESI-MS/MS detection was achieved in positive mode under multiple reaction monitoring (MRM) mode. The calibration curves showed good linearity in the range of 5-100 microg/L with the correlation coefficients more than 0.99 for the 20 psychoactive drugs. The limits of quantification (LOQ, S/N > or = 10) for the 20 psychoactive drugs were 5 microg/kg. The average recoveries of the 20 psychoactive drugs spiked in blank pork at three levels of 5, 10 and 50 microg/kg were 66.8%-97.2% with the relative standard deviations from 4.2% to 12.4%. The meth od is suitable for the simultaneous determination of the 20 psychoactive drugs in pork with the characteristics of easy operation, high accuracy and precision.
authors.forename: Ting
authors.initials: T
authors.lastname: Sun
authors.forename: Luxiao
authors.initials: L
authors.lastname: Wang
authors.forename: Sanmei
authors.initials: S
authors.lastname: Zeng
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.forename: Lijuan
authors.initials: L
authors.lastname: Yan
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D011619:Psychotropic Drugs / Q000032:analysis*; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D013552:Swine; D053719:Tandem Mass Spectrometry
chemical_list: D011619:Psychotropic Drugs