title: Distribution of bla(OXA-23), ISAba , Aminoglycosides resistant genes among burned & ICU patients in Tehran and Sari, Iran.
abstract: BACKGROUND
  Multidrug resistant strains of Acinetobacter baumannii (MDR-AB) have emerged as alarming nosocomial pathogens among patients admitted to Intensive Care Unit and burned patients. The aim of this study was to determine the susceptibility of A. baumannii isolates, the carbapenems resistance patterns bla(OXA-23) and also ISAba elements of A. baumannii isolates among burned and ICU patients in Tehran and Sari, Iran.
  
  
  METHODS
  In this study, 100 A. baumannii isolates from burned and ICU patients in Tehran and Sari (Iran) during 2013 were tested for determination of antimicrobials susceptibility by the disc-diffusion method on Mueller Hinton agar recommended by the guidelines of Clinical and Laboratory Standards Institute (CLSI), and frequency bla(OXA-23) carbapenemase genes, and insertion elements ISAba genes were studied by PCR method.
  
  
  RESULTS
  The highest rates of susceptibility were observed with Colistin (88.7%), Tigecycline (82.2%), Imipenem (67%) and ISAba (32.2%). The extensively drug-resistance and pan drug-resistance were observed in 37.1% and 8.1% isolates, respectively. Results indicated among isolates resistant to Aminoglycoside and Carbapenem, the highest resistance was observed to Streptomycin (90%) ' and the most sensitivity was to Imipenem (67%).
  
  
  CONCLUSIONS
  This is the most study that attempted to detect Acinetobacter baumanii the insertion elements ISAba , bla(OXA-23) and aminoglycosides resistance in MDR-AB isolates from burned and ICU patients in Iran. In a timely manner, antimicrobial resistance surveillance and strict infection control strategies are still lacking in burn ward and ICU in Iran, despite the alarming emergence of MDR-AB strains, particularly among those isolates that are not susceptible to Colistin. The results of this study are consistent with a recent report in which a number of combinations exhibited potent activity against Multidrug resistant strains of A. baumannii (MDR-AB).
authors.affiliation: Department of Microbiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. mnasrolahei@yahoo.com.
authors.forename: Mohtaram
authors.initials: M
authors.lastname: Nasrolahei
authors.affiliation: Department of Microbiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. zahedi.bahador@gmail.com.
authors.forename: Bahador
authors.initials: B
authors.lastname: Zahedi
authors.affiliation: Departments of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. ab.bahador@gmail.com.
authors.forename: Abbas
authors.initials: A
authors.lastname: Bahador
authors.affiliation: Applied Microbiology Research center and Microbiology Department, Baqiyatallah University Medical of Sciences, Tehran, Iran. saghi1443@gmail.com.
authors.forename: Hossein
authors.initials: H
authors.lastname: Saghi
authors.affiliation: Department of Microbiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. soudehkholdi@yahoo.com.
authors.forename: Soudeh
authors.initials: S
authors.lastname: Kholdi
authors.affiliation: Department of Genetic' Science and Research Branch, Islamic Azad University, Tabriz, East Azerbaijan, Iran. nedajalalvand@gmail.com.
authors.forename: Neda
authors.initials: N
authors.lastname: Jalalvand
authors.affiliation: Applied Microbiology Research center and Microbiology Department, Baqiyatallah University Medical of Sciences, Tehran, Iran. esm114@gmail.com.
authors.forename: Davoud
authors.initials: D
authors.lastname: Esmaeili
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D000617:Aminoglycosides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002056:Burns / Q000150:complications*; D016638:Critical Illness; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D004251:DNA Transposable Elements; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial; D006801:Humans; D007362:Intensive Care Units; D007492:Iran / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D047090:beta-Lactams / Q000494:pharmacology*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D047090:beta-Lactams