title: Fusobacterium hwasookii sp. nov., Isolated from a Human Periodontitis Lesion.
abstract: In this study, we classified the five strains (ChDC F128(T), ChDC F145, ChDC F174, ChDC F206, and ChDC F300) as a novel species of genus Fusobacterium by DNA-DNA hybridization and multi-locus phylogenetic analysis (MLPA), based on a single sequence (24,715 bp) of 22 concatenated housekeeping genes, with morphological and chemotaxonomic characteristics. DNA-DNA hybridization data showed that the values of genomic relatedness between ChDC F128(T) and each of the other novel strains were ranged from 79.0 to 82.6 %, while those of genomic relatedness between ChDC F128(T) and type strain of each of subspecies of F. nucleatum or Fusobacterium periodonticum were ranged from 40.9 to 54.4 %. MLPA revealed that the 5 strains were clustered as one group and clearly discriminated with F. nucleatum and F. periodonticum with 100 % bootstrap value. The DNA G+C content of the five novel strains were ranged from 26.9 to 27.0 mol%. The cellular fatty acid analysis of clinical isolates and type strains revealed C14:0, C16:0, and cis-9 C16:1 as the major fatty acids. The cell wall peptidoglycan of the 5 strains was comprised of meso-lanthionine. These results show that the 5 strains are novel species and belong to the genus Fusobacterium. Strain ChDC F128(T) (=KCOM 1249(T) = KCTC 5108(T) = JCM 30218(T)) is suggested to be the type strain of a novel species of genus Fusobacterium, for which the name Fusobacterium hwasookii sp. nov. is proposed.
authors.affiliation: Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea.
authors.forename: Eugene
authors.initials: E
authors.lastname: Cho
authors.forename: Soon-Nang
authors.initials: SN
authors.lastname: Park
authors.forename: Yun Kyong
authors.initials: YK
authors.lastname: Lim
authors.forename: Yeseul
authors.initials: Y
authors.lastname: Shin
authors.forename: Jayoung
authors.initials: J
authors.lastname: Paek
authors.forename: Cheol Ho
authors.initials: CH
authors.lastname: Hwang
authors.forename: Young-Hyo
authors.initials: YH
authors.lastname: Chang
authors.forename: Joong-Ki
authors.initials: JK
authors.lastname: Kook
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial; D005673:Fusobacterium / Q000145:classification / Q000235:genetics / Q000378:metabolism; D005674:Fusobacterium Infections / Q000382:microbiology*; D020043:Genes, Essential; D006801:Humans; D058885:Multilocus Sequence Typing; D010518:Periodontitis / Q000382:microbiology*; D010802:Phylogeny
chemical_list: D004269:DNA, Bacterial