title: Ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharides from pumpkin (Cucurbita moschata).
abstract: An efficient ultrasound-assisted enzymatic extraction (UAEE) of Cucurbita moschata polysaccharides (CMCP) was established and the CMCP antioxidant activities were studied. The UAEE operating parameters (extraction temperature, ultrasonic power, pH, and liquid-to-material ratio) were optimized using the central composite design (CCD) and the mass transfer kinetic study in UAEE procedure was used to select the optimal extraction time. Enzymolysis and ultrasonication that were simultaneously conducted was selected as the UAEE synergistic model and the optimum extraction conditions with a maximum polysaccharide yield of 4.33 ± 0.15% were as follows: extraction temperature, 51.5 °C; ultrasonic power, 440 W; pH, 5.0; liquid-to-material ratio, 5.70:1 mL/g; and extraction time, 20 min. Evaluation of the antioxidant activity in vitro suggested that CMCP has good potential as a natural antioxidant used in the food or medicine industry because of their high reducing power and positive radical scavenging activity for DPPH radical.
authors.affiliation: College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, PR China.
authors.forename: Junxiang
authors.initials: J
authors.lastname: Zhu
authors.affiliation: College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, PR China.
authors.forename: Wenchao
authors.initials: W
authors.lastname: Diao
authors.affiliation: College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, PR China. Electronic address: qauwcr@126.com.
authors.forename: Chengrong
authors.initials: C
authors.lastname: Wang
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology*; D028464:Cucurbita / Q000201:enzymology*; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D013016:Sound
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D011134:Polysaccharides
keywords: Antioxidant activity; Cucurbita moschata; Polysaccharide; Response surface methodology; Ultrasound-assisted enzymatic extraction