title: Effect of methotrexate upon antigen-induced arthritis of the rabbit temporomandibular joint.
abstract: BACKGROUND
  Juvenile idiopathic arthritis (JIA) of the temporomandibular joint (TMJ) can cause severe growth disturbances of the craniomandibular system. Antigen-induced arthritis (AIA) of the rabbit TMJ is simulating the inflammatory process of the TMJ in JIA. The aim of this study was to investigate the effect of a systemic administration of methotrexate (MTX) on AIA in rabbits by means of three different histological staining methods.
  
  
  METHODS
  After sensitization, a bilateral arthritis of the TMJ was induced by an intra-articular administration of ovalbumin in 12 New Zealand white rabbits aged 10 weeks. From the 13th week of age, six of the 12 rabbits received weekly intramuscular injections of MTX, and the other six animals remained without therapy. Another six animals served as controls, receiving no treatment or intra-articular injections at all. After euthanasia at the age of 22 weeks, all TMJs were retrieved en bloc. Sagittal sections were cut and stained with haematoxylin-eosin (H-E), Safranin-O for the evaluation of the Mankin score and tartrate-resistant acid phosphatase (TRAP).
  
  
  RESULTS
  In the arthritis group, a chronic inflammation with degeneration of the articular cartilage was visible. In the MTX group, the signs of cartilage degeneration were significantly reduced compared with the arthritis group. In contrast, the joints in the control group were inconspicuous. A correlation between the Mankin score and TRAP-positive cells could be found.
  
  
  CONCLUSIONS
  Systemic administration of MTX seems to have a positive effect upon the inflammatory process in the rabbit TMJ but fails to eliminate the sign of arthritis completely.
authors.affiliation: Department of Orthodontics, Dentofacial Orthopedics and Pedodontics, Center for Dental and Craniofacial Sciences, Charité - Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Smbat
authors.initials: S
authors.lastname: Rafayelyan
authors.affiliation: Department of Orthodontics, Dentofacial Orthopedics and Pedodontics, Center for Dental and Craniofacial Sciences, Charité - Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Meyer
authors.affiliation: Department of Oral Structural Biology, Center for Dental and Craniofacial Sciences, Charité - Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Ralf J
authors.initials: RJ
authors.lastname: Radlanski
authors.affiliation: German Rheumatism Research Centre Berlin, Berlin, Germany.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Minden
authors.affiliation: Department of Orthodontics, Dentofacial Orthopedics and Pedodontics, Center for Dental and Craniofacial Sciences, Charité - Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Paul-Georg
authors.initials: PG
authors.lastname: Jost-Brinkmann
authors.affiliation: Department of Orthodontics, Dentofacial Orthopedics and Pedodontics, Center for Dental and Craniofacial Sciences, Charité - Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Präger
mesh_terms: D000818:Animals; D018501:Antirheumatic Agents / Q000494:pharmacology*; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000473:pathology; D002358:Cartilage, Articular / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D007249:Inflammation / Q000473:pathology; D007270:Injections, Intra-Articular; D008727:Methotrexate / Q000494:pharmacology*; D010047:Ovalbumin / Q000008:administration & dosage; D011817:Rabbits; D011897:Random Allocation; D013704:Temporomandibular Joint / Q000473:pathology; D013705:Temporomandibular Joint Disorders / Q000188:drug therapy* / Q000473:pathology
chemical_list: D018501:Antirheumatic Agents; D010047:Ovalbumin; D008727:Methotrexate
keywords: antigen-induced arthritis; histology; methotrexate; rabbit; temporomandibular joint