title: Coenzyme Q10 protects hair cells against aminoglycoside.
abstract: It is well known that the production of free radicals is associated with sensory cell death induced by an aminoglycoside. Many researchers have reported that antioxidant reagents protect sensory cells in the inner ear, and coenzyme Q10 (CoQ10) is an antioxidant that is consumed as a health food in many countries. The purpose of this study was to investigate the role of CoQ10 in mammalian vestibular hair cell death induced by aminoglycoside. Cultured utricles of CBA/CaN mice were divided into three groups (control group, neomycin group, and neomycin + CoQ10 group). In the neomycin group, utricles were cultured with neomycin (1 mM) to induce hair cell death. In the neomycin + CoQ10 group, utricles were cultured with neomycin and water-soluble CoQ10 (30-0.3 µM). Twenty-four hours after exposure to neomycin, the cultured tissues were fixed, and vestibular hair cells were labeled using an anti-calmodulin antibody. Significantly more hair cells survived in the neomycin + CoQ10 group than in the neomycin group. These data indicate that CoQ10 protects sensory hair cells against neomycin-induced death in the mammalian vestibular epithelium; therefore, CoQ10 may be useful as a protective drug in the inner ear.
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Kazuma
authors.initials: K
authors.lastname: Sugahara
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Hirose
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Takefumi
authors.initials: T
authors.lastname: Mikuriya
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hashimoto
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Eiju
authors.initials: E
authors.lastname: Kanagawa
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Hara
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Shimogori
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamashita
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002147:Calmodulin / Q000032:analysis; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D018069:Hair Cells, Vestibular / Q000166:cytology* / Q000473:pathology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009355:Neomycin / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012444:Saccule and Utricle / Q000166:cytology; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000447:Aldehydes; D000900:Anti-Bacterial Agents; D002147:Calmodulin; D020011:Protective Agents; D017382:Reactive Oxygen Species; D014451:Ubiquinone; C024989:coenzyme Q10; D009355:Neomycin; C027576:4-hydroxy-2-nonenal