title: Metabolic activity and genetic diversity of microbial communities inhabiting the rhizosphere of halophyton plants.
abstract: A preliminary study was conducted to compare the community level physiological profile (CLPP) and genetic diversity of rhizosphere microbial communities of four plant species growing nearby Kiskunság soda ponds, namely Böddi-szék, Kelemen-szék and Zab-szék. CLPP was assessed by MicroResp method using 15 different substrates while Denaturing Gradient Gel Electrophoresis (DGGE) was used to analyse genetic diversity of bacterial communities. The soil physical and chemical properties were quite different at the three sampling sites. Multivariate statistics (PCA and UPGMA) revealed that Zab-szék samples could be separated according to their genetic profile from the two others which might be attributed to the geographical location and perhaps the differences in soil physical properties. Böddi-szék samples could be separated from the two others considering the metabolic activity which could be explained by their high salt and low humus contents. The number of bands in DGGE gels was related to the metabolic activity, and positively correlated with soil humus content, but negatively with soil salt content. The main finding was that geographical location, soil physical and chemical properties and the type of vegetation were all important factors influencing the metabolic activity and genetic diversity of rhizosphere microbial communities.
authors.affiliation: Eötvös Loránd University Department of Microbiology Pázmány P. sétány 1/C H-1117 Budapest Hungary.
authors.forename: Agnes
authors.initials: A
authors.lastname: Bárány
authors.affiliation: Hungarian Academy of Sciences Institute for Soil Sciences and Agricultural Chemistry, Centre for Agricultural Research Herman Ottó út 15 H-1022 Budapest Hungary.
authors.forename: Tibor
authors.initials: T
authors.lastname: Szili-Kovács
authors.affiliation: Eötvös Loránd University Department of Microbiology Pázmány P. sétány 1/C H-1117 Budapest Hungary.
authors.forename: Gergely
authors.initials: G
authors.lastname: Krett
authors.affiliation: Hungarian Academy of Sciences Institute for Soil Sciences and Agricultural Chemistry, Centre for Agricultural Research Herman Ottó út 15 H-1022 Budapest Hungary.
authors.forename: Anna
authors.initials: A
authors.lastname: Füzy
authors.affiliation: Eötvös Loránd University Department of Microbiology Pázmány P. sétány 1/C H-1117 Budapest Hungary.
authors.forename: Károly
authors.initials: K
authors.lastname: Márialigeti
authors.affiliation: Eötvös Loránd University Department of Microbiology Pázmány P. sétány 1/C H-1117 Budapest Hungary.
authors.forename: Andrea K
authors.initials: AK
authors.lastname: Borsodi
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D014644:Genetic Variation; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D058441:Rhizosphere; D055051:Salt-Tolerant Plants / Q000382:microbiology*; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D012987:Soil
keywords: DGGE; MicroResp; SIR; rhizosphere bacterial communities; soda soil