title: [Effects and mechanisms of punicosides on acute alcoholic liver damage in mice].
abstract: OBJECTIVE
  To evaluate the protective effects of punicosides on alcohol induced acute liver injury in mice and its possible mechanisms as well.
  
  
  METHODS
  The 60 mice were randomly divided into normal control, model group, three dose groups of punicosides with low, medium and high, then there is silibinin group. Three dose groups of punicosides and silibinin were given in advance by gavage for 4 weeks, then the mouse model of alcoholic acute liver injury was established. The serum levels of ALT, AST and TG were determined, and the mice were killed to calculate somatic index of liver, thymus as well as spleen. MDA, SOD, GSH-Px and GSH-ST were detected in the liver homogenate. Histopathological changes of the liver were observed by HE staining. The expression of MCP-1 and NF-kappaB in the liver tissues were detected by immunohistochemistry.
  
  
  RESULTS
  Mid and high dose of punicosides reduced the liver index in mice significantly, improved liver steatosis, decreased the level of ALT, AST and TG in serum and the content of MDA in liver homogenate, furthermore the two dose groups increased the activity of SOD, GSH-Px and GSH-ST, inhibited the expression of MCP-1 and NF-kappaB in liver tissue.
  
  
  CONCLUSIONS
  Punicosides can protect the acute liver damage induced by alcohol.
authors.forename: Fang
authors.initials: F
authors.lastname: Wei
authors.forename: Rui-Jie
authors.initials: RJ
authors.lastname: Xu
authors.forename: Shi-Yi
authors.initials: SY
authors.lastname: Cai
authors.forename: Zhan-Zhan
authors.initials: ZZ
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
mesh_terms: D000438:Alcohols / Q000009:adverse effects*; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D018932:Chemokine CCL2 / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism
chemical_list: D000438:Alcohols; C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D008315:Malondialdehyde