title: [The change of NOS in pulmonary oxygen toxicity induced by different oxygen pressure].
abstract: OBJECTIVE
  Long time exhaled oxygen will induced oxygen toxicity. Some studies had found that different pathology may exised in normobaric and hyperbaric pulmonary oxygen toxicity, and nitric oxide synthase (NOS) may play a role. In this study, we discussed the change of NOS in normobaric and hyperbaric pulmonary oxygen toxicity.
  
  
  METHODS
  Sixty male SD rats were randomly divided into 6 groups (n = 10), exposed to 1 ATA (atmosphere absolute), 1.5 ATA, 2 ATA, 2.5 ATA and 3 ATA, 100% oxygen for 56, 20, 10, 8, 6 hours respectively. Rats were exposed to air as control. After exposure, the protein in bronchoalveolar lavage fluid (BALF), the wet/dry weight of lung and the expression of eNOS, nNOS in lung were defined.
  
  
  RESULTS
  As compared to air group, the protein in BALF, the wet/dry of lung were significantly elevated in 1.0 ATA group, while these changes were not so obviously in the other groups, and these changes in hyperbaric oxygen group (approximately 1.0 ATA) were significantly decreased as compared with nonnrmobaric oxygen group (1.0 ATA). The expression of nNOS were not changed in normobaric and hyperbaric pulmonary oxygen toxicity, while the expression of eNOS was significantly decreased in 2 ATA group, and significantly elevated in 2.5 ATA and 3 ATA group.
  
  
  CONCLUSIONS
  The expression of eNOS can change when exposed to different pressures of oxygen.
authors.forename: Ai-Zi
authors.initials: AZ
authors.lastname: Liu
authors.forename: Xiao-Chen
authors.initials: XC
authors.lastname: Bao
authors.forename: Yi-Qun
authors.initials: YQ
authors.lastname: Fang
authors.forename: Zhong-Na
authors.initials: ZN
authors.lastname: Sang
authors.forename: Hua-Jiang
authors.initials: HJ
authors.lastname: Li
authors.forename: Wan-Qi
authors.initials: WQ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D008168:Lung / Q000378:metabolism; D008297:Male; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D010100:Oxygen / Q000506:poisoning*; D011312:Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D052248:Nitric Oxide Synthase Type I; D052250:Nitric Oxide Synthase Type III; C496322:Nos1 protein, rat; C496316:Nos3 protein, rat; D010100:Oxygen