title: [Acteoside enhances expression of neurotrophin-3 in brain tissues of subacute aging mice induced by D-galactose combined with aluminum trichloride].
abstract: OBJECTIVE
  To study the effect of acteoside on the expression of neurotrophin-3 (NT-3) in brain tissues of subacute aging mice induced by D-galactose (D-Gal) combined with aluminum trichloride (AlCl3).
  
  
  METHODS
  Female Kunming mice were randomly divided into vehicle control group, D-Gal combined with AlCl3 group , vitamin E group, piracetam group and acteoside groups [30, 60, 120 mg/(kg.d)]. Mice in D-Gal combined with AlCl3, vitamin E, piracetam and acteoside groups were injected intraperitoneally (i.p.) with D-Gal [60 mg/(kg.d)] and AlCl3 [5 mg/(kg.d)] for 90 days to induce the subacute aging models. The real-time quantitative PCR, Western blotting and immunohistochemistry were respectively used to detect the expression of NT-3 mRNA and protein in cerebral cortex and hippocampus of mouse brain.
  
  
  RESULTS
  D-Gal and AlCl3 caused a significant reduction of NT-3 in cerebral cortex and hippocampus. Acteoside, vitamin E and piracetam increased the decreased expression of NT-3 induced by D-Gal and AlCl3, and the difference was statistically significant (P<0.05 or P<0.01).
  
  
  CONCLUSIONS
  Acteoside can up-regulate the expression of brain NT-3 in D-Gal plus AlCl3 treated mice.
authors.affiliation: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.affiliation: Prescription Laboratory of Xinjiang Traditional Uyghur Medicine, Xinjiang Institute of Traditional Uighur Medicine, Urumqi 830049, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Peng
authors.affiliation: Prescription Laboratory of Xinjiang Traditional Uyghur Medicine, Xinjiang Institute of Traditional Uighur Medicine, Urumqi 830049, China.
authors.forename: Shixia
authors.initials: S
authors.lastname: Huo
authors.affiliation: Prescription Laboratory of Xinjiang Traditional Uyghur Medicine, Xinjiang Institute of Traditional Uighur Medicine, Urumqi 830049, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.affiliation: Prescription Laboratory of Xinjiang Traditional Uyghur Medicine, Xinjiang Institute of Traditional Uighur Medicine, Urumqi 830049, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002712:Chlorides / Q000494:pharmacology*; D005260:Female; D005690:Galactose / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D005960:Glucosides / Q000494:pharmacology*; D007150:Immunohistochemistry; D051379:Mice; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology; D010636:Phenols / Q000494:pharmacology*; D010889:Piracetam / Q000494:pharmacology; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects; D014810:Vitamin E / Q000494:pharmacology; D014815:Vitamins / Q000494:pharmacology; D059808:Polyphenols
chemical_list: D000077410:Aluminum Chloride; D017607:Aluminum Compounds; D000975:Antioxidants; D002712:Chlorides; D005690:Galactose; D005960:Glucosides; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; D010636:Phenols; D010889:Piracetam; D014810:Vitamin E; D014815:Vitamins; C518911:neurotropin 3, mouse; C058956:acteoside; D059808:Polyphenols