title: Electricity generation and wastewater treatment of oil refinery in microbial fuel cells using Pseudomonas putida.
abstract: Microbial fuel cells (MFCs) represent a novel platform for treating wastewater and at the same time generating electricity. Using Pseudomonas putida (BCRC 1059), a wild-type bacterium, we demonstrated that the refinery wastewater could be treated and also generate electric current in an air-cathode chamber over four-batch cycles for 63 cumulative days. Our study indicated that the oil refinery wastewater containing 2213 mg/L (ppm) chemical oxygen demand (COD) could be used as a substrate for electricity generation in the reactor of the MFC. A maximum voltage of 355 mV was obtained with the highest power density of 0.005 mW/cm² in the third cycle with a maximum current density of 0.015 mA/cm² in regard to the external resistor of 1000 Ω. A maximum coulombic efficiency of 6 × 10⁻²% was obtained in the fourth cycle. The removal efficiency of the COD reached 30% as a function of time. Electron transfer mechanism was studied using cyclic voltammetry, which indicated the presence of a soluble electron shuttle in the reactor. Our study demonstrated that oil refinery wastewater could be used as a substrate for electricity generation.
authors.affiliation: Department of Life Science, National Chung Cheng University, 168 University Road, Minhsiung, Chia-Yi 62102, Taiwan. dipmajumder1@gmail.com.
authors.forename: Dip
authors.initials: D
authors.lastname: Majumder
authors.affiliation: Department of Earth and Environmental Sciences, National Chung Cheng University 168, University Rd., Min-Hsiung, Chia-Yi 62102, Taiwan. jyoti_maity@yahoo.com.
authors.forename: Jyoti Prakash
authors.initials: JP
authors.lastname: Maity
authors.affiliation: Department of Life Science, National Chung Cheng University, 168 University Road, Minhsiung, Chia-Yi 62102, Taiwan. biomjt@ccu.edu.tw.
authors.forename: Min-Jen
authors.initials: MJ
authors.lastname: Tseng
authors.affiliation: Department of Chemical Engineering, University Institute of Chemical Technology, Nathalal Parekh Road, Matunga East, Mumbai, Maharashtra 400019, India. vanita.nimje@gmail.com.
authors.forename: Vanita Roshan
authors.initials: VR
authors.lastname: Nimje
authors.affiliation: Department of Life Science, National Chung Cheng University, 168 University Road, Minhsiung, Chia-Yi 62102, Taiwan. biohrc@ccu.edu.tw.
authors.forename: Hau-Ren
authors.initials: HR
authors.lastname: Chen
authors.affiliation: Department of Biotechnology, National Kaohsiung Normal University, No. 62, Shenjhong Rd., Yanchao Township, Kaohsiung County 82444, Taiwan. cheng@nknucc.nknu.edu.tw.
authors.forename: Chien-Cheng
authors.initials: CC
authors.lastname: Chen
authors.affiliation: Department of Earth and Environmental Sciences, National Chung Cheng University 168, University Rd., Min-Hsiung, Chia-Yi 62102, Taiwan. seichyo@ccu.edu.tw.
authors.forename: Young-Fo
authors.initials: YF
authors.lastname: Chang
authors.affiliation: Department of Hotel and Restaurant Management, Chia-Nan University of Pharmacy and Science, Tainan 71751, Taiwan. t10344@mail.chna.edu.tw.
authors.forename: Tsui-Chu
authors.initials: TC
authors.lastname: Yang
authors.affiliation: Department of Earth and Environmental Sciences, National Chung Cheng University 168, University Rd., Min-Hsiung, Chia-Yi 62102, Taiwan. chien-yen.chen@oriel.oxon.org.
authors.forename: Chen-Yen
authors.initials: CY
authors.lastname: Chen
mesh_terms: D001674:Bioelectric Energy Sources / Q000382:microbiology; D057919:Biological Oxygen Demand Analysis; D002617:Chemical Industry / Q000379:methods*; D004560:Electricity; D004566:Electrodes; D004867:Equipment Design; D059392:Petroleum Pollution / Q000517:prevention & control*; D016958:Pseudomonas putida / Q000502:physiology*; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater; D014874:Water Pollutants, Chemical; D018508:Water Purification / Q000379:methods*
chemical_list: D062065:Wastewater; D014874:Water Pollutants, Chemical