title: Plk4 phosphorylates Ana2 to trigger Sas6 recruitment and procentriole formation.
abstract: Centrioles are 9-fold symmetrical structures at the core of centrosomes and base of cilia whose dysfunction has been linked to a wide range of inherited diseases and cancer. Their duplication is regulated by a protein kinase of conserved structure, the C. elegans ZYG-1 or its Polo-like kinase 4 (Plk4) counterpart in other organisms. Although Plk4's centriolar partners and mechanisms that regulate its stability are known, its crucial substrates for centriole duplication have never been identified. Here we show that Drosophila Plk4 phosphorylates four conserved serines in the STAN motif of the core centriole protein Ana2 to enable it to bind and recruit its Sas6 partner. Ana2 and Sas6 normally load onto both mother and daughter centrioles immediately after their disengagement toward the end of mitosis to seed procentriole formation. Nonphosphorylatable Ana2 still localizes to the centriole but can no longer recruit Sas6 and centriole duplication fails. Thus, following centriole disengagement, recruitment of Ana2 and its phosphorylation by Plk4 are the earliest known events in centriole duplication to recruit Sas6 and thereby establish the architecture of the new procentriole engaged with its parent.
authors.affiliation: Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK. Electronic address: nsd23@cam.ac.uk.
authors.forename: Nikola S
authors.initials: NS
authors.lastname: Dzhindzhev
authors.affiliation: Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK.
authors.forename: George
authors.initials: G
authors.lastname: Tzolovsky
authors.affiliation: Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Lipinszki
authors.affiliation: Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK.
authors.forename: Sandra
authors.initials: S
authors.lastname: Schneider
authors.affiliation: Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK.
authors.forename: Ramona
authors.initials: R
authors.lastname: Lattao
authors.affiliation: Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK.
authors.forename: Jingyan
authors.initials: J
authors.lastname: Fu
authors.affiliation: Laboratory of Mass Spectrometry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Debski
authors.affiliation: Laboratory of Mass Spectrometry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Dadlez
authors.affiliation: Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK. Electronic address: dmg25@cam.ac.uk.
authors.forename: David M
authors.initials: DM
authors.lastname: Glover
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000378:metabolism*; D002502:Centrioles / Q000378:metabolism*; D004330:Drosophila / Q000378:metabolism* / Q000648:ultrastructure; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016415:Sequence Alignment
chemical_list: C547835:Ana2 protein, Drosophila; D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D008869:Microtubule-Associated Proteins; C522062:Sas-6 protein, Drosophila; D017346:Protein Serine-Threonine Kinases; C522064:Sak protein, Drosophila