title: Development and validation of stability-indicating HPLC method for simultaneous determination of meropenem and potassium clavulanate.
abstract: A stability-indicating LC assay method was developed and validated for a simultaneous determination of meropenem and potassium clavulanate in the presence of degradation products formed during acid-base hydrolysis, oxidation and thermolysis. The isocratic RP-HPLC method was developed with a LiChrospher RP-18 (250 mm x 4.6 mm, 5 microm) column and gradient elution of 12 mmol/L ammonium acetate and acetonitrile. The flow rate of the mobile phase was 1.0 mL/min, the detection wavelength 220 nm and the temperature 303 K. The method was validated with regard to linearity, accuracy, precision, selectivity and robustness, and was applied successfully for the determination of meropenem and potassium clavulanate separately as well as jointly in pharmaceutical formulations.
authors.forename: Przemysław
authors.initials: P
authors.lastname: Zalewski
authors.forename: Judyta
authors.initials: J
authors.lastname: Cielecka-Piontek
authors.forename: Magdalena
authors.initials: M
authors.lastname: Paczkowska
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D019818:Clavulanic Acid / Q000008:administration & dosage / Q000032:analysis* / Q000737:chemistry; D004355:Drug Stability; D004359:Drug Therapy, Combination; D000077731:Meropenem; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013845:Thienamycins / Q000008:administration & dosage / Q000032:analysis* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D013845:Thienamycins; D019818:Clavulanic Acid; D000077731:Meropenem