title: Switch from αvβ5 to αvβ6 integrin is required for CD9-regulated keratinocyte migration and MMP-9 activation.
abstract: Our previous research found that tetraspanin CD9 is downregulated in migrating epidermis during wound healing, and CD9 downregulation contributes to keratinocyte migration via matrix metalloproteinase-9 (MMP-9) activation. However, little is known about the mechanisms involved in CD9-regulated keratinocyte migration and MMP-9 activation. In this study, we revealed that the expressions of integrin subunits β5 and β6 were regulated by CD9. Furthermore, CD9 silencing triggered the switch from αvβ5 to αvβ6 integrin in HaCaT keratinocytes and CD9 overexpression reversed the switch. Importantly, integrin αvβ6 functional blocking antibody 10D5 significantly inhibited CD9 silencing-induced keratinocyte migration and MMP-9 activation, suggesting that the switch from αvβ5 to αvβ6 integrin plays a key role in CD9-regulated cell migration and MMP-9 activation in keratinocytes.
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Xupin
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Burn and Plastic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Teng
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Guo
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Dongxia
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Jiaping
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University, Chongqing, China. Electronic address: yshuang.tmmu@gmail.com.
authors.forename: Yuesheng
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000906:Antibodies / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D002465:Cell Movement; D015536:Down-Regulation; D004789:Enzyme Activation; D020868:Gene Silencing; D006801:Humans; D016023:Integrins / Q000276:immunology / Q000378:metabolism*; D015603:Keratinocytes / Q000166:cytology*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D019030:Receptors, Vitronectin / Q000378:metabolism*; D060245:Tetraspanin 29 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*
chemical_list: D000906:Antibodies; D000951:Antigens, Neoplasm; D016023:Integrins; D019030:Receptors, Vitronectin; D060245:Tetraspanin 29; C064660:integrin alphaVbeta5; C073878:integrin alphavbeta6; D020780:Matrix Metalloproteinase 9
keywords: CD9; Integrin; Keratinocyte; Matrix metalloproteinase-9; Migration