title: Tissue-protective effects of NKG2A in immune-mediated clearance of virus infection.
abstract: Virus infection triggers a CD8(+) T cell response that aids in virus clearance, but also expresses effector functions that may result in tissue injury. CD8(+) T cells express a variety of activating and inhibiting ligands, though regulation of the expression of inhibitory receptors is not well understood. The ligand for the inhibitory receptor, NKG2A, is the non-classical MHC-I molecule Qa1(b), which may also serve as a putative restricting element for the T cell receptors of purported regulatory CD8(+) T cells. We have previously shown that Qa1(b)-null mice suffer considerably enhanced immunopathologic lung injury in the context of CD8(+) T cell-mediated clearance of influenza infection, as well as evidence in a non-viral system that failure to ligate NKG2A on CD8(+) effector T cells may represent an important component of this process. In this report, we examine the requirements for induction of NKG2A expression, and show that NKG2A expression by CD8(+) T cells occurs as a result of migration from the MLN to the inflammatory lung environment, irrespective of peripheral antigen recognition. Further, we confirmed that NKG2A is a mediator in limiting immunopathology in virus infection using mice with a targeted deletion of NKG2A, and infecting the mutants with two different viruses, influenza and adenovirus. In neither infection is virus clearance altered. In influenza infection, the enhanced lung injury was associated with increased chemoattractant production, increased infiltration of inflammatory cells, and significantly enhanced alveolar hemorrhage. The primary mechanism of enhanced injury was the loss of negative regulation of CD8(+) T cell effector function. A similar effect was observed in the livers of mutant mice infected intravenously with adenovirus. These results demonstrate the immunoregulatory role of CD8(+) NKG2A expression in virus infection, which negatively regulates T cell effector functions and contributes to protection of tissue integrity during virus clearance.
authors.affiliation: Department of Medicine, Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire, United States of America.
authors.forename: Kenneth H
authors.initials: KH
authors.lastname: Ely
authors.affiliation: Department of Medicine, Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire, United States of America.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Matsuoka
authors.affiliation: Department of Medicine, Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire, United States of America.
authors.forename: Matthew P
authors.initials: MP
authors.lastname: DeBerge
authors.affiliation: Department of Medicine, Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire, United States of America.
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Ruby
authors.affiliation: Department of Medicine, Yale University School of Medicine, New Haven, Connecticut, United States of America.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Medicine, Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire, United States of America.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Schneider
authors.affiliation: Department of Medicine, Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire, United States of America.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Beirne B. Carter Center for Immunology Research, University of Virginia School of Medicine, Charlottesville, Virginia, United States of America.
authors.forename: Young S
authors.initials: YS
authors.lastname: Hahn
authors.affiliation: Department of Medicine, Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire, United States of America; Department of Microbiology/Immunology, Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire, United States of America.
authors.forename: Richard I
authors.initials: RI
authors.lastname: Enelow
mesh_terms: D000256:Adenoviridae / Q000472:pathogenicity; D000257:Adenoviridae Infections / Q000276:immunology / Q000473:pathology / Q000662:veterinary; D019264:Adoptive Transfer; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D055786:Gene Knockout Techniques; D009980:Influenza A virus / Q000472:pathogenicity; D055370:Lung Injury / Q000276:immunology / Q000473:pathology* / Q000821:virology; D051379:Mice; D055654:NK Cell Lectin-Like Receptor Subfamily C / Q000235:genetics* / Q000378:metabolism*; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000473:pathology / Q000662:veterinary
chemical_list: C527057:Klrc1 protein, mouse; D055654:NK Cell Lectin-Like Receptor Subfamily C