title: Sample preparation methods for LC-MS-based global aqueous metabolite profiling.
abstract: Metabolite extraction is a key step in metabolomic analyses, particularly for untargeted studies. The extraction determines the types of metabolites that will be detected and the analytical platform to be used. In this chapter we describe two protocols aimed at detecting polar metabolites from biological samples; the first is aimed at detecting reduced species by LC/MS, and the second satisfies the requirements for both NMR and LC/MS analysis simultaneously.
authors.affiliation: Metabolomics Platform, Spanish Biomedical Research Center in Diabetes and Associated Metabolic Disorders (CIBERDEM), Barcelona, Spain.
authors.forename: Antoni
authors.initials: A
authors.lastname: Beltran
authors.forename: Sara
authors.initials: S
authors.lastname: Samino
authors.forename: Oscar
authors.initials: O
authors.lastname: Yanes
mesh_terms: D000818:Animals; D005591:Chemical Fractionation / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008099:Liver / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods*; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D044967:Serum / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water