title: Aberrant production of Th1/Th2/Th17-related cytokines in serum of C57BL/6 mice after short-term formaldehyde exposure.
abstract: Previous studies have shown that formaldehyde (FA) could cause immunotoxicity by changing the number of T lymphocytes and that cytokines play a pivotal role in the regulation of T lymphocytes. However, the previously used cytokine detection methods are difficult to use in the measurement of several cytokines in a small amount of sample for one test. Therefore, the cytometric bead array (CBA) technique was used. CBA showed better analytical efficiency and sensitivity than the previous methods. C57BL/6 mice were exposed to the control (normal saline), low FA concentration (0.5 mg/kg), and high FA concentration (2 mg/kg) for 1 week or 1 month. The contents of cytokines, including Th1-related cytokines (IL-2, IFN-γ, and tumor necrosis factor), Th2-related cytokines (IL-4, IL-6, and IL-10), and Th17-related cytokines (IL-17A), were measured by using the BD FACS Canto II Flow Cytometer and analyzed by FCAP ArrayTM Software. Th1/Th2/Th17-related cytokines showed a slightly decreasing trend after low FA exposure. Conversely, a significantly increasing trend was found after high FA exposure. Th1/Th2/Th17-related cytokines all serve important functions in the immune reactions in mice after FA exposure.
authors.affiliation: Key Laboratory of Environmental Medicine Engineering of Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. why_314614@163.com.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wei
authors.affiliation: Key Laboratory of Environmental Medicine Engineering of Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. kehom123@126.com.
authors.forename: Kehong
authors.initials: K
authors.lastname: Tan
authors.affiliation: Key Laboratory of Environmental Medicine Engineering of Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. sunrongli5318@gmail.com.
authors.forename: Rongli
authors.initials: R
authors.lastname: Sun
authors.affiliation: Key Laboratory of Environmental Medicine Engineering of Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. lhyin@seu.edu.cn.
authors.forename: Lihong
authors.initials: L
authors.lastname: Yin
authors.affiliation: Key Laboratory of Environmental Medicine Engineering of Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. 101011288@seu.edu.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Environmental Medicine Engineering of Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. yppu@seu.edu.cn.
authors.forename: Yuepu
authors.initials: Y
authors.lastname: Pu
mesh_terms: D000818:Animals; D016207:Cytokines / Q000097:blood / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods; D005557:Formaldehyde / Q000633:toxicity*; D015386:Hazardous Substances / Q000633:toxicity*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects* / Q000276:immunology
chemical_list: D016207:Cytokines; D015386:Hazardous Substances; D005557:Formaldehyde