title: Acetone, butanol, and ethanol production from gelatinized cassava flour by a new isolates with high butanol tolerance.
abstract: To obtain native strains resistant to butanol toxicity, a new isolating method and serial enrichment was used in this study. With this effort, mutant strain SE36 was obtained, which could withstand 35g/L (compared to 20g/L of the wild-type strain) butanol challenge. Based on 16s rDNA comparison, the mutant strain was identified as Clostridium acetobutylicum. Under the optimized condition, the phase shift was smoothly triggered and fermentation performances were consequently enhanced. The maximum total solvent and butanol concentration were 23.6% and 24.3%, respectively higher than that of the wild-type strain. Furthermore, the correlation between butanol produced and the butanol tolerance was investigated, suggesting that enhancing butanol tolerance could improve butanol production. These results indicate that the simple but effective isolation method and acclimatization process are a promising technique for isolation and improvement of butanol tolerance and production.
authors.affiliation: College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, China; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Han-Guang
authors.initials: HG
authors.lastname: Li
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Fred Kwame
authors.initials: FK
authors.lastname: Ofosu
authors.affiliation: College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
authors.forename: Kun-Tai
authors.initials: KT
authors.lastname: Li
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Qiu-Ya
authors.initials: QY
authors.lastname: Gu
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address: yijiangnanl@163.com.
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Yu
mesh_terms: D000096:Acetone / Q000378:metabolism*; D000440:Butanols / Q000378:metabolism* / Q000633:toxicity; D046969:Clostridium acetobutylicum / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D017895:Manganese Compounds; D002365:Manihot; D013431:Sulfates; D003313:Zea mays
chemical_list: D000440:Butanols; D017895:Manganese Compounds; D013431:Sulfates; D000096:Acetone; D000431:Ethanol; C039798:manganese sulfate
keywords: ABE fermentation; Butanol tolerance; Cassava flour; Clostridium acetobutylicum; Phase shift