title: Direct enzyme-substrate affinity determination by real-time hyperpolarized (13)C-MRS.
abstract: A specialized kinetic analysis of real-time hyperpolarized [1,1,2,2-D4, 1-(13)C]choline (13)C-magnetic resonance spectroscopy enabled the determination of initial rates of metabolic enzyme activity (choline oxidase), enzyme-substrate affinity (Km), and inhibition. In a clinical MRI scanner, metabolite levels lower than 16 μM were detected at a temporal resolution of 1 s.
authors.affiliation: Department of Medical Biophysics, Western University, London, Ontario, Canada.
authors.forename: L J
authors.initials: LJ
authors.lastname: Friesen-Waldner
authors.forename: C N
authors.initials: CN
authors.lastname: Wiens
authors.forename: T P
authors.initials: TP
authors.lastname: Wade
authors.forename: K
authors.initials: K
authors.lastname: Thind
authors.forename: K P
authors.initials: KP
authors.lastname: Sinclair
authors.forename: Y
authors.initials: Y
authors.lastname: Hovav
authors.forename: J M
authors.initials: JM
authors.lastname: Gomori
authors.forename: J
authors.initials: J
authors.lastname: Sosna
authors.forename: C A
authors.initials: CA
authors.lastname: McKenzie
authors.forename: R
authors.initials: R
authors.lastname: Katz-Brull
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D002794:Choline / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy
chemical_list: D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D002794:Choline