title: Streptomyces bohaiensis sp. nov., a novel actinomycete isolated from Scomberomorus niphonius in the Bohai Sea.
abstract: A novel actinomycete strain, designated 11A07(T), was isolated from young Scomberomorus niphonius in the Bohai Sea. Basic local alignment search tool analyses showed that this isolate had the highest 16S rRNA gene sequence similarity of 97.41% with Streptomyces rimosus subsp. paromomycinus DSM 41429(T). Phylogenetic tree revealed that strain 11A07(T) formed a distinct lineage clustered with Streptomyces panacagri Gsoil 519(T), Streptomyces sodiiphilus YIM 80305(T) and Streptomyces albus subsp. albus NRRL B-2365(T) having similarities of 97.30%, 97.10% and 96.83%, respectively. Multilocus sequence analysis further demonstrated that the new isolate was different from the selected representatives of Streptomyces as a separate phylogenetic line. Strain 11A07(T) produced straight or rectiflexibile spore chains with smooth surface, white aerial mycelia and brown diffusible pigments on international streptomyces project 2 medium. Maximum tolerated NaCl concentration for growth was 11.0%. Whole-cell sugars were mannose, ribose, glucose, galactose and xylose. The predominant menaquinones were MK-9(H2), MK-9(H4) and MK-9 (H6). The fatty-acid profile contained iso-C16:0, C18:0 10-methyl (tuberculostearic acid) and anteiso-C17:0 as the major compositions. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unknown phospholipid. The G+C content of the genomic DNA was 71.4 mol%. These morphological, phenotypic and chemotaxonomic properties showed that strain 11A07(T) could be readily distinguished from the most closely related members of the genus Streptomyces. Thus, based on the polyphasic taxonomic data, strain 11A07(T) (=JCM 19630(T)=CCTCC AA 2013020(T)=KCTC 29263(T)) represents a novel species within the genus Streptomyces, for which the name Streptomyces bohaiensis sp. nov. is proposed.
authors.affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China.
authors.forename: Hua-Qi
authors.initials: HQ
authors.lastname: Pan
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, China.
authors.forename: Dao-Feng
authors.initials: DF
authors.lastname: Zhang
authors.affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China.
authors.forename: Su-Ya
authors.initials: SY
authors.lastname: Yu
authors.affiliation: 1] Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, China [2] School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Hanoi, Vietnam.
authors.forename: Thi-Nhan
authors.initials: TN
authors.lastname: Khieu
authors.affiliation: School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Hanoi, Vietnam.
authors.forename: Chu Ky
authors.initials: CK
authors.lastname: Son
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China.
authors.forename: Jiang-Chun
authors.initials: JC
authors.lastname: Hu
authors.affiliation: 1] Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, China [2] State Key Laboratory of Biocontrol, Key Laboratory of Biodiversity Dynamics and Conservation of Guangdong Higher Education Institutes, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D000818:Animals; D001482:Base Composition / Q000235:genetics; D002241:Carbohydrates / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D058885:Multilocus Sequence Typing; D009792:Oceans and Seas; D010473:Perciformes / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D002241:Carbohydrates; D005227:Fatty Acids; D008055:Lipids; D012336:RNA, Ribosomal, 16S