title: Effects of different dietary phospholipid levels on growth performance, fatty acid composition, PPAR gene expressions and antioxidant responses of blunt snout bream Megalobrama amblycephala fingerlings.
abstract: A 60-day feeding trial was conducted to evaluate the effects of different levels of dietary phospholipid (PL) from soybean lecithin on growth performance, liver fatty acid composition, peroxisome proliferator-activated receptor (PPAR) gene expression levels and antioxidant responses of blunt snout bream fingerlings. Fish (average initial weight 0.35 ± 0.01 g) were fed five experimental diets containing the following inclusion levels of PL: 0, 2, 4, 6 and 8%. Results showed that final body weight, weight gain and specific growth rate increased significantly (P < 0.05) as dietary PL level increased from 0 to 6%, meanwhile the survival was not affected by dietary PL supplementation. Increasing dietary PL level significantly (P < 0.05) increased in 20:4n-6 content in neutral lipid of liver, indicating fish had the capacity to convert C18 to C20 and C22 by elongation and desaturation. The expression levels of PPAR-α and PPAR-γ and the activities of catalase, superoxide dismutase and glutathione peroxidase in liver were significantly (P < 0.05) increased, and liver thiobarbituric acid reactive substances value was decreased with dietary PL supplementation up to 6% compared with the control. Therefore, it was concluded that supplementation of 6% (18.8 g kg(-1), polar lipid of diet) PL could improve growth performance of blunt snout bream fingerlings.
authors.affiliation: College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.forename: Songqian
authors.initials: S
authors.lastname: Huang
mesh_terms: D000704:Analysis of Variance; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002374:Catalase / Q000378:metabolism; D003530:Cyprinidae / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D004032:Diet / Q000662:veterinary*; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D048788:Growth and Development / Q000187:drug effects*; D008099:Liver / Q000378:metabolism; D047492:Peroxisome Proliferator-Activated Receptors / Q000235:genetics / Q000378:metabolism*; D010743:Phospholipids / Q000008:administration & dosage / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D017931:DNA Primers; D005227:Fatty Acids; D047492:Peroxisome Proliferator-Activated Receptors; D010743:Phospholipids; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase