title: Structural studies of arabinan-rich pectic polysaccharides from Abies sibirica L. Biological activity of pectins of A. sibirica.
abstract: Highly branched arabinan-rich pectic polysaccharides, containing 84% of arabinose, was extracted from wood greenery of Abies sibirica L. The structure of arabinan was studied by the 1D and 2D NMR spectroscopy. The macromolecule backbone was represented mainly by RG-I (molar ratio GalA:Rha ∼ 1.3:1) patterns with high degree of rhamnose branching. Side chains were comprised of 1,5-linked α-L-Araf residues (the major part of polymer mass), 1,3,5-di-O- and 1,2,3,5-tri-O-linked α-L-Araf residues, confirming the presence of highly branched 1,5-α-L-arabinan. Although most L-Araf were in α-anomeric form, minor terminal β-L-Araf-(1 →... was detected. 1,4-β-D-linked Galp residues found in the side chains account for minor AG-I or 1,4-galactan, as compared to arabinan. A tentative structure was proposed. Polysaccharides obtained from Siberian fir greenery were screened for biological activity. Galacturonan had a strongest stimulating effect on germination and growth rate of seeds, germs and roots of Triticum aestivum, Avena sativa, and Secale cereale.
authors.affiliation: Institute of Chemistry, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, Pervomaiskaya str., 48, Syktyvkar 167982, Russia.
authors.forename: Evgeny G
authors.initials: EG
authors.lastname: Shakhmatov
authors.affiliation: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky prospekt 47, Moscow 119991, Russia.
authors.forename: Philip V
authors.initials: PV
authors.lastname: Toukach
authors.affiliation: Institute of Physiology, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, Pervomaiskaya str., 50, Syktyvkar 167982, Russia.
authors.forename: Capital Ie Cyrilliclena А
authors.initials: CIe
authors.lastname: Michailowa
authors.affiliation: Institute of Chemistry, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, Pervomaiskaya str., 48, Syktyvkar 167982, Russia. Electronic address: makarowa.elena-ma@yandex.ru.
authors.forename: Elena N
authors.initials: EN
authors.lastname: Makarova
mesh_terms: D028202:Abies / Q000737:chemistry; D010368:Pectins / Q000737:chemistry; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D014908:Triticum / Q000187:drug effects* / Q000254:growth & development
chemical_list: D011134:Polysaccharides; C030080:araban; D010368:Pectins
keywords: Abies sibirica; Arabinan; NMR spectroscopy; Pectin; Wood greenery; β-l-Araf