title: CpG island methylator phenotype and prognosis of colorectal cancer in Northeast China.
abstract: OBJECTIVE
  To investigate the association between CpG island methylator phenotype (CIMP) and the overall survival of sporadic colorectal cancer (CRC) in Northeast China.
  
  
  METHODS
  282 sporadic CRC patients were recruited in this study. We selected MLH1, MGMT, p16, APC, MINT1, MINT31, and RUNX3 as the CIMP panel markers. The promoter methylation was assessed by methylation sensitive high resolution melting (MS-HRM). Proportional hazards-regression models were fitted with computing hazard ratios (HR) and the corresponding 95% confidence intervals (95% CI).
  
  
  RESULTS
  12.77% (36/282) of patients were CIMP-0, 74.1% (209/282) of patients were CIMP-L, and 13.12% (37/282) of patients were CIMP-H. The five-year survival of the 282 CRC patients was 58%. There was significant association between APC gene promoter methylation and CRC overall survival (HR = 1.61; 95% CI: 1.05-2.46; P = 0.03). CIMP-H was significantly associated with worse prognosis compared to CIMP-0 (HR = 3.06; 95% CI: 1.19-7.89; P = 0.02) and CIMP-L (HR = 1.97; 95% CI: 1.11-3.48; P = 0.02), respectively. While comparing with the combine of CIMP-L and CIMP-0 (CIMP-L/0), CIMP-H also presented a worse prognosis (HR = 2.31; 95% CI: 1.02-5.24; P = 0.04).
  
  
  CONCLUSIONS
  CIMP-H may be a predictor of a poor prognosis of CRC in Northeast China patients.
authors.affiliation: Department of Epidemiology, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Epidemiology, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Fulan
authors.initials: F
authors.lastname: Hu
authors.affiliation: Department of Epidemiology, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Yibaina
authors.identifier: 0000-0003-3651-4663
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Epidemiology, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Yao
authors.affiliation: Department of Epidemiology, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Zuoming
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Epidemiology, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Surgery, Cancer Hospital of Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Guizhi
authors.initials: G
authors.lastname: Sun
authors.affiliation: Department of Surgery, Cancer Hospital of Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Bin-Bin
authors.initials: BB
authors.lastname: Cui
authors.affiliation: Department of Surgery, Cancer Hospital of Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Xinshu
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Epidemiology, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Yashuang
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002681:China; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D010641:Phenotype; D011379:Prognosis; D011401:Promoter Regions, Genetic; D016019:Survival Analysis