title: [¹H NMR metabonomics study of pancreatic extracts from insulin-resistant rats induced by fructose feeding].
abstract: OBJECTIVE
  To study the metabolic changes of pancreatic extracts from insulin-resistant rats induced by fructose feeding using nuclear magnetic resonance ¹H spectroscopy (¹H NMR).
  
  
  METHODS
  Sixteen Wistar rats were divided equally into control group and model group and given water and 10% fructose water for 8 weeks, respectively. The pancreatic tissues were then obtained for H NMR spectra analysis and principal component analysis (PCA).
  
  
  RESULTS
  Compared with the control rats, the rats in the model group showed significantly increased creatine, betaine/TMAO, taurine, glycine and myo-inositol and decreased levels of lipids, lactate, glutamate, choline and GPC/PC.
  
  
  CONCLUSIONS
  ¹H NMR and pattern recognition can define the metabolic characteristics of the pancreatic tissue extracts from insulin-resistant rats and provide reliable metabolic evidence for studying the mechanisms of insulin resistance at the molecular level.
authors.affiliation: ollege of Traditional Chinese Medicine, 2School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou 510006, China. E-mail: hhxxwll@163.com.
authors.forename: Linlin
authors.initials: L
authors.lastname: Wang
authors.forename: Lingyun
authors.initials: L
authors.lastname: Zheng
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Ali
authors.initials: A
authors.lastname: Chen
authors.forename: Cuihuan
authors.initials: C
authors.lastname: Qiu
authors.forename: Jingfen
authors.initials: J
authors.lastname: Xu
authors.forename: Yongxia
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000818:Animals; D005632:Fructose; D007333:Insulin Resistance; D055432:Metabolomics; D010184:Pancreatic Extracts / Q000737:chemistry*; D025341:Principal Component Analysis; D066244:Proton Magnetic Resonance Spectroscopy; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010184:Pancreatic Extracts; D005632:Fructose