title: Chemical structures and hepatoprotective effects of constituents from Cassia auriculata leaves.
abstract: An 80% aqueous acetone extract of Cassia auriculata leaves was found to show a protective effect on D-galactosamine-induced cytotoxicity in primary cultured mouse hepatocytes. From the 80% aqueous acetone extract, we isolated a new benzocoumarin glycoside, avaraoside I (1), and a new flavanol dimer, avaraol I (2), together with 29 known constituents. The structures of the new compounds were elucidated on the basis of chemical and physicochemical evidence. In addition, three isolated compounds, pseudosemiglabrin (15, 0.0011%), (2S)-7,4'-dihydroxyflavan(4β→8)-catechin (22, 0.00075%), and (2S)-7,4'-dihydroxyflavan(4β→8)-gallocatechin (23, 0.092%), displayed hepatoprotective effects equivalent to that of the hepatoprotective agent, silybin.
authors.affiliation: Kyoto Pharmaceutical University.
authors.forename: Seikou
authors.initials: S
authors.lastname: Nakamura
authors.forename: Fengming
authors.initials: F
authors.lastname: Xu
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Ninomiya
authors.forename: Souichi
authors.initials: S
authors.lastname: Nakashima
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Oda
authors.forename: Toshio
authors.initials: T
authors.lastname: Morikawa
authors.forename: Osamu
authors.initials: O
authors.lastname: Muraoka
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: Hisashi
authors.initials: H
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D002366:Cassia / Q000737:chemistry* / Q000378:metabolism; D002478:Cells, Cultured; D005688:Galactosamine / Q000633:toxicity; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008968:Molecular Conformation; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D020011:Protective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts; D020011:Protective Agents; D005688:Galactosamine