title: Pantoea coffeiphila sp. nov., cause of the 'potato taste' of Arabica coffee from the African Great Lakes region.
abstract: Six isolates recovered from coffee seeds giving off a potato-like flavour were studied. Gene sequencing (rrs and rpoB) showed they belong to the genus Pantoea. By DNA-DNA hybridization, the isolates constituted a genomic species with less than 17% relatedness to 96 strains representing enterobacterial species. Multilocus sequence analysis (gyrB, rpoB, atpD and infB genes) showed the isolates to represent a discrete species of the genus Pantoea. Nutritional versatility of the novel species was poor. The novel species is proposed as Pantoea coffeiphila sp.nov. and its type strain is Ca04(T) ( =CIP 110718(T) =DSM 28482(T)).
authors.affiliation: CIRAD, UMR Qualisud, Montpellier, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Gueule
authors.affiliation: CIRAD, UMR Qualisud, Montpellier, France.
authors.forename: Gérard
authors.initials: G
authors.lastname: Fourny
authors.affiliation: Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris, France.|Unité Biodiversité des Bactéries Pathogènes Emergentes, Institut Pasteur, Paris, France.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Ageron
authors.affiliation: Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence, Institut Pasteur, Paris, France.|Unité Biodiversité des Bactéries Pathogènes Emergentes, Institut Pasteur, Paris, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Le Flèche-Matéos
authors.affiliation: Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence, Institut Pasteur, Paris, France.
authors.forename: Mathias
authors.initials: M
authors.lastname: Vandenbogaert
authors.affiliation: Unité Biodiversité des Bactéries Pathogènes Emergentes, Institut Pasteur, Paris, France.
authors.forename: Patrick A D
authors.initials: PAD
authors.lastname: Grimont
authors.affiliation: CIRAD, UR Bioagresseurs, Montpellier, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Cilas
mesh_terms: D015373:Bacterial Typing Techniques; D002063:Burundi; D003069:Coffee / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005506:Food Contamination; D005516:Food Microbiology; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D009693:Nucleic Acid Hybridization; D020636:Pantoea / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013649:Taste
chemical_list: D003069:Coffee; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S