title: Simultaneous determination of 16 phenolic constituents in Spatholobi Caulis by high performance liquid chromatography/electrospray ionization triple quadrupole mass spectrometry.
abstract: Spatholobi Caulis, the vine stem of Spatholobus suberectus Dunn, has been widely used in traditional Chinese and folk medicines for treatment of irregular menstruation, blood deficiency and rheumatalgia in clinic. In this study, an accurate and reliable high performance liquid chromatography/electrospray ionization triple quadrupole mass spectrometry (HPLC-MS/MS) method was established for simultaneous determination of 16 phenolic bioactive constituents, including five flavanols, seven isoflavonoids, three flavanones and one chalcone in Spatholobi Caulis. The method validation results exhibited that the developed method had desirable specificity, linearity, precision and accuracy. The quantitative analysis results showed that flavanols were the abundant constituents in Spatholobi Caulis. Moreover, principal component analysis (PCA) was performed to assess the quality variation of samples collected from different regions in China. The PCA results indicated the quantitative analysis based on HPLC-MS/MS is a feasible method for quality assessment and control of Spatholobi Caulis.
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China.
authors.forename: Long
authors.initials: L
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China.
authors.forename: Li
authors.initials: L
authors.lastname: Duan
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China. Electronic address: liuehu2011@163.com.
authors.forename: E-Hu
authors.initials: EH
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China. Electronic address: liping2004@126.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007887:Fabaceae / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D018547:Plant Stems / Q000737:chemistry; D011786:Quality Control; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D004365:Drugs, Chinese Herbal; D010636:Phenols
keywords: HPLC–MS/MS; Phenolic compounds; Principal component analysis; Quantitative analysis; Spatholobi Caulis