title: Elimination of Pasteurella pneumotropica from a mouse barrier facility by using a modified enrofloxacin treatment regimen.
abstract: Multiple NOD. Cg-Prkdc(scid)Il2rg(tm1Wjl)Tg(HLA-A2.1)Enge/Sz (NSG/A2) transgenic mice maintained in a mouse barrier facility were submitted for necropsy to determine the cause of facial alopecia, tachypnea, dyspnea, and sudden death. Pneumonia and soft-tissue abscesses were observed, and Pasteurella pneumotropica biotype Jawetz was consistently isolated from the upper respiratory tract, lung, and abscesses. Epidemiologic investigation within the facility revealed presence of this pathogen in mice generated or rederived by the intramural Genetically Engineered Mouse Model (GEMM) Core but not in mice procured from several approved commercial vendors. Epidemiologic data suggested the infection originated from female or vasectomized male ND4 mice obtained from a commercial vendor and then comingled by the GEMM Core to induce pseudopregnancy in female mice for embryo implantation. Enrofloxacin delivered in drinking water (85 mg/kg body weight daily) for 14 d was sufficient to clear bacterial infection in normal, breeding, and immune-deficient mice without the need to change the antibiotic water source. This modified treatment regimen was administered to 2400 cages of mice to eradicate Pasteurella pneumotropica from the facility. Follow-up PCR testing for P. pneumotropica biotype Jawetz remained uniformly negative at 2, 6, 12, and 52 wk after treatment in multiple strains of mice that were originally infected. Together, these data indicate that enrofloxacin can eradicate P. pneumotropica from infected mice in a less labor-intensive approach that does not require breeding cessation and that is easily adaptable to the standard biweekly cage change schedule for individually ventilated cages.
authors.affiliation: University Animal Care, University of Arizona, Tucson, Arizona, USA.
authors.forename: Justin W
authors.initials: JW
authors.lastname: Towne
authors.affiliation: University Animal Care, University of Arizona, Tucson, Arizona, USA.
authors.forename: April M
authors.initials: AM
authors.lastname: Wagner
authors.affiliation: Department of Immunobiology, University of Arizona, Tucson, Arizona, USA.
authors.forename: Kurt J
authors.initials: KJ
authors.lastname: Griffin
authors.affiliation: Department of Immunobiology, University of Arizona, Tucson, Arizona, USA.
authors.forename: Adam S
authors.initials: AS
authors.lastname: Buntzman
authors.affiliation: Department of Immunobiology, University of Arizona, Tucson, Arizona, USA.
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Frelinger
authors.affiliation: University Animal Care, University of Arizona, Tucson, Arizona, USA besselsd@email.arizona.edu.
authors.forename: David G
authors.initials: DG
authors.lastname: Besselsen
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D000830:Animals, Laboratory; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D000077422:Enrofloxacin; D005260:Female; D024841:Fluoroquinolones / Q000008:administration & dosage*; D008297:Male; D016688:Mice, Inbred NOD; D010326:Pasteurella Infections / Q000188:drug therapy / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D044142:Pasteurella pneumotropica / Q000302:isolation & purification*; D012376:Rodent Diseases / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D000077422:Enrofloxacin