title: Entry of Bombyx mori nucleopolyhedrovirus into BmN cells by cholesterol-dependent macropinocytic endocytosis.
abstract: Bombyx mori nucleopolyhedrovirus (BmNPV) is a serious viral pathogen of silkworm, and no drug or specific protection against BmNPV infection is available at present time. Although functions of most BmNPV genes were depicted in recent years, knowledge on the mechanism of BmNPV entry into insect cells is still limited. Here BmNPV cell entry mechanism is investigated by different endocytic inhibitor application and subcellular analysis. Results indicated that BmNPV enters BmN cells by clathrin-independent macropinocytic endocytosis, which is mediated by cholesterol in a dose-dependent manner, and cholesterol replenishment rescued the BmNPV infection partially.
authors.affiliation: College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, PR China; Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang, Jiangsu 212018, PR China. Electronic address: jshuang@just.edu.cn.
authors.forename: Jinshan
authors.initials: J
authors.lastname: Huang
authors.affiliation: College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, PR China; Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang, Jiangsu 212018, PR China.
authors.forename: Bifang
authors.initials: B
authors.lastname: Hao
authors.affiliation: College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Cheng
authors.affiliation: College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liang
authors.affiliation: College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, PR China; Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang, Jiangsu 212018, PR China.
authors.forename: Xingjia
authors.initials: X
authors.lastname: Shen
authors.affiliation: Department of Microbiology, Miami University, Oxford, OH 45056, USA.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Cheng
mesh_terms: D000818:Animals; D012831:Bombyx / Q000378:metabolism / Q000648:ultrastructure / Q000821:virology*; D002460:Cell Line; D021961:Cell Membrane Structures / Q000378:metabolism / Q000648:ultrastructure; D002784:Cholesterol / Q000378:metabolism*; D002966:Clathrin / Q000378:metabolism; D019476:Insect Proteins / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D010873:Pinocytosis / Q000502:physiology*; D053586:Virus Internalization
chemical_list: D002966:Clathrin; D019476:Insect Proteins; D008563:Membrane Lipids; D002784:Cholesterol
keywords: BmNPV; Cholesterol; Endocytosis; Macropinocytosis