title: Emodin-loaded magnesium silicate hollow nanocarriers for anti-angiogenesis treatment through inhibiting VEGF.
abstract: The applications of anti-VEGF (vascular endothelial growth factor) treatment in ophthalmic fields to inhibit angiogenesis have been widely documented in recent years. However, the hydrophobic nature of many agents makes its delivery difficult in practice. Therefore, the aim of the present study was to introduce a new kind of hydrophobic drug carrier by employing nanoparticles with a hollow structure inside. Followed by the synthesis and characterization of magnesium silicate hollow spheres, cytotoxicity was evaluated in retina capillary endothelial cells. The loading and releasing capacity were tested by employing emodin, and the effect on VEGF expression was performed at the gene and protein level. Finally, an investigation on angiogenesis was carried on fertilized chicken eggs. The results indicated that the magnesium silicate nanoparticles had low toxicity. Emodin-MgSiO3 can inhibit the expression of both VEGF gene and protein effectively. Angiogenesis of eggs was also reduced significantly. Based on the above results, we concluded that magnesium silicate hollow spheres were good candidates as drug carriers with enough safety.
authors.affiliation: Department of Ophthalmology, First Hospital of Jilin University, Changchun 130021, China. jlurenhua@yeah.net.
authors.forename: Hua
authors.initials: H
authors.lastname: Ren
authors.affiliation: Department of Ophthalmology, Second Hospital of Jilin University, Changchun 130021, China. jluzhuchao@yeah.net.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Department of Ophthalmology, People's Hospital of Changchun City, Changchun 130021, China. jlulizhaohui@yeah.net.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, First Hospital of Jilin University, Changchun 130021, China. jluyangwei@yeah.net.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.affiliation: Department of Ophthalmology, First Hospital of Jilin University, Changchun 130021, China. jlusonge@yeah.net.
authors.forename: E
authors.initials: E
authors.lastname: Song
mesh_terms: D020533:Angiogenesis Inhibitors / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D000818:Animals; D002196:Capillaries / Q000166:cytology; D002478:Cells, Cultured; D002642:Chick Embryo; D004337:Drug Carriers; D004642:Emodin / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D042783:Endothelial Cells / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D017633:Magnesium Silicates; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity; D054118:Nanospheres / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity; D018919:Neovascularization, Physiologic / Q000187:drug effects; D015861:Retinal Neovascularization / Q000188:drug therapy / Q000517:prevention & control; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors*
chemical_list: D020533:Angiogenesis Inhibitors; D004337:Drug Carriers; D017633:Magnesium Silicates; D053769:Nanocapsules; D042461:Vascular Endothelial Growth Factor A; C005013:Florisil; D004642:Emodin