title: Cisplatin and paclitaxel target significant long noncoding RNAs in laryngeal squamous cell carcinoma.
abstract: The objectives of this study were to identify specific long noncoding RNAs (lncRNAs) in laryngeal squamous cell carcinoma (LSCC) and to clarify the function of cisplatin and paclitaxel on the confirmed laryngeal cancer lncRNAs. Fifty-four pairs of laryngeal tumor and adjacent normal tissue were collected. Candidate lncRNAs were searched in authorized databases. The significant lncRNAs were identified and confirmed through high-output real-time PCR. Chemotherapy assay evaluated the influences of cisplatin and paclitaxel on the significant lncRNAs. Thirty-seven cancer-related candidate lncRNAs were selected. Three up-expressed and two down-expressed significant lncRNAs were identified and confirmed. The expressions of lncRNA CDKN2B-AS1, HOTAIR and MALAT1 were dramatically reduced with the increasing concentration of cisplatin and paclitaxel and also lengthening of the treatment duration. Cisplatin and paclitaxel have target function on significant lncRNAs in LSCC, which presents novel molecular targets to cure LSCC patients and also leads an orientation for developing new drugs.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Affiliated Eye and ENT Hospital of Fudan University, #83, Fenyang Road, Shanghai, 200031, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xin
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.forename: Jia-meng
authors.initials: JM
authors.lastname: Huang
authors.forename: Lei
authors.initials: L
authors.lastname: Tao
authors.forename: Lei
authors.initials: L
authors.lastname: Cheng
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000008:administration & dosage*; D016503:Drug Delivery Systems / Q000379:methods; D005260:Female; D006258:Head and Neck Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D017239:Paclitaxel / Q000008:administration & dosage*; D062085:RNA, Long Noncoding / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D062085:RNA, Long Noncoding; D017239:Paclitaxel; D002945:Cisplatin