title: Parathyroid hormone 1-34 enhances extracellular matrix deposition and organization during flexor tendon repair.
abstract: Parathyroid hormone (PTH) 1-34 is known to enhance fracture healing. Tendon repair is analogous to bone healing in its dependence on the proliferation and differentiation of mesenchymal stem cells, matrix formation, and tissue remodeling.(1,2,3) We hypothesized that PTH 1-34 enhances tendon healing in a flexor digitorum longus (FDL) tendon repair model. C57Bl/6J mice were treated with either intraperitoneal PTH 1-34 or vehicle-control (PBS). Tendons were harvested at 3-28 days for histology, gene expression, and biomechanical testing. The metatarsophalangeal joint range of motion was reduced 1.5-2-fold in PTH 1-34 mice compared to control mice. The gliding coefficient, a measure of adhesion formation, was 2-3.5-fold higher in PTH 1-34 mice. At 14 days post-repair, the tensile strength was twofold higher in PTH 1-34 specimens, but at 28 days there were no differences. PTH 1-34 mice had increased fibrous tissue deposition that correlated with elevated expression of collagens and fibronectin as seen on quantitative PCR. PTH 1-34 accelerated the deposition of reparative tissue but increased adhesion formation.
authors.affiliation: The Center for Musculoskeletal Research, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, New York, 14642.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Lee
authors.forename: Richard D
authors.initials: RD
authors.lastname: Southgate
authors.forename: Youssef M
authors.initials: YM
authors.lastname: Farhat
authors.forename: Alayna E
authors.initials: AE
authors.lastname: Loiselle
authors.forename: Warren C
authors.initials: WC
authors.lastname: Hammert
authors.forename: Hani A
authors.initials: HA
authors.lastname: Awad
authors.forename: Regis J
authors.initials: RJ
authors.lastname: O'Keefe
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena / Q000187:drug effects / Q000502:physiology; D003094:Collagen / Q000378:metabolism; D005109:Extracellular Matrix / Q000187:drug effects* / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D008297:Male; D008683:Metatarsophalangeal Joint / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D010281:Parathyroid Hormone / Q000494:pharmacology* / Q000627:therapeutic use; D016059:Range of Motion, Articular / Q000187:drug effects / Q000502:physiology; D013708:Tendon Injuries / Q000188:drug therapy / Q000378:metabolism* / Q000601:surgery; D013710:Tendons / Q000187:drug effects* / Q000378:metabolism* / Q000601:surgery; D013718:Tensile Strength / Q000187:drug effects / Q000502:physiology; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D005353:Fibronectins; D010281:Parathyroid Hormone; D003094:Collagen
keywords: PTH 1-34; adhesions; biomechanics; forteo; tendon healing