title: Blood-brain barrier dysfunction in mice induced by lipopolysaccharide is attenuated by dapsone.
abstract: Blood-brain barrier (BBB) dysfunction is a key event in the development of many central nervous system (CNS) diseases, such as septic encephalopathy and stroke. 4,4'-Diaminodiphenylsulfone (DDS, Dapsone) has displayed neuroprotective effect, but whether DDS has protective role on BBB integrity is not clear. This study was designed to examine the effect of DDS on lipopolysaccharide (LPS)-induced BBB disruption and oxidative stress in brain vessels. Using in vivo multiphoton imaging, we found that DDS administration significantly restored BBB integrity compromised by LPS. DDS also increased the expression of tight junction proteins occludin, zona occludens-1 (ZO-1) and claudin-5 in brain vessels. Level of reactive oxygen species (ROS) was reduced by DDS treatment, which may due to decreased nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity and NOX2 expression. Our results showed that LPS-induced BBB dysfunction could be attenuated by DDS, indicated that DDS has a therapeutic potential for treating CNS infection and other BBB related diseases.
authors.affiliation: Neuroscience Research Institute and Department of Neurobiology, Key Laboratory for Neuroscience, Ministry of Education and Ministry of Public Health, Health Science Center, Peking University, Beijing, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Neuroscience Research Institute and Department of Neurobiology, Key Laboratory for Neuroscience, Ministry of Education and Ministry of Public Health, Health Science Center, Peking University, Beijing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Neuroscience Research Institute and Department of Neurobiology, Key Laboratory for Neuroscience, Ministry of Education and Ministry of Public Health, Health Science Center, Peking University, Beijing, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhan
authors.affiliation: Center of Medical and Health Analysis, Peking University, Beijing, China.
authors.forename: Qihua
authors.initials: Q
authors.lastname: He
authors.affiliation: Neuroscience Research Institute and Department of Neurobiology, Key Laboratory for Neuroscience, Ministry of Education and Ministry of Public Health, Health Science Center, Peking University, Beijing, China.
authors.forename: Yawei
authors.initials: Y
authors.lastname: Tong
authors.affiliation: Neuroscience Research Institute and Department of Neurobiology, Key Laboratory for Neuroscience, Ministry of Education and Ministry of Public Health, Health Science Center, Peking University, Beijing, China.
authors.forename: Xiaosheng
authors.initials: X
authors.lastname: Tian
authors.affiliation: Neuroscience Research Institute and Department of Neurobiology, Key Laboratory for Neuroscience, Ministry of Education and Ministry of Public Health, Health Science Center, Peking University, Beijing, China.
authors.forename: Hecheng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Neuroscience Research Institute and Department of Neurobiology, Key Laboratory for Neuroscience, Ministry of Education and Ministry of Public Health, Health Science Center, Peking University, Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Neuroscience Research Institute and Department of Neurobiology, Key Laboratory for Neuroscience, Ministry of Education and Ministry of Public Health, Health Science Center, Peking University, Beijing, China.
authors.forename: Yaoyun
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Neuroscience Research Institute and Department of Neurobiology, Key Laboratory for Neuroscience, Ministry of Education and Ministry of Public Health, Health Science Center, Peking University, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.affiliation: Peking University Third Hospital, Beijing, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Guo
authors.affiliation: Peking University Third Hospital, Beijing, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Fan
authors.affiliation: Peking University Third Hospital, Beijing, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Han
authors.affiliation: Neuroscience Research Institute and Department of Neurobiology, Key Laboratory for Neuroscience, Ministry of Education and Ministry of Public Health, Health Science Center, Peking University, Beijing, China; Peking University Third Hospital, Beijing, China. Electronic address: dchui@bjmu.edu.cn.
authors.forename: Dehua
authors.initials: D
authors.lastname: Chui
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D003622:Dapsone / Q000494:pharmacology*; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D003622:Dapsone
keywords: Blood–brain barrier integrity; Dapsone; Lipopolysaccharide