title: Solidification of floating organic drop liquid-phase microextraction cell fishing with gas chromatography-mass spectrometry for screening bioactive components from Amomum villosum Lour.
abstract: In this study, a novel solidification of floating organic drop liquid-phase microextraction cell fishing with gas chromatography-mass spectrometry (SFOD-LPME-CF-GC-MS) method was established and used to screen, isolate and analyze bioactive components from Amomum villosum Lour. extract. Through comparision of its effect on the models of normal cell and inflammatory cells, anti-inflammatory active components of essential oil from A. villosum Lour. were readily screened, and the components obtained are in agreement with related pharmacological articles. SFOD-LPME-CF-GC-MS was used to analyze the interaction of A. villosum Lour. extracts with normal and lipopolysaccharide-stimulated RAW264.7 macrophage cells. The effect of A. villosum Lour. essential oil extracts in the LPS-stimulated RAW264.7 model were also assessed in terms of cytotoxicity and nitric oxide production as an indication of bioactivity. Three potentially bioactive components were identified, demonstrating that SFOD-LPME-CF-GC-MS can be used successfully in the drug-screening process. This approach avoids the requirement for protein precipitation, but more importantly, generates a high concentration ratio, allowing analysis of trace components in traditional Chinese medicines. SFOD-LPME-CF-GC-MS is a simple, fast, effective and reliable method for the screening and analysis of bioactive components, and it can be extended to screen other bioactive components from TCMs.
authors.affiliation: School of Pharmaceutical Sciences, Sun Yet-sen University, Guangzhou, 510006, People's Republic of China.
authors.forename: Xue
authors.initials: X
authors.lastname: Xue
authors.forename: Depo
authors.initials: D
authors.lastname: Yang
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wang
authors.forename: Xinjun
authors.initials: X
authors.lastname: Xu
authors.forename: Longping
authors.initials: L
authors.lastname: Zhu
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D035801:Amomum / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D059627:Liquid Phase Microextraction / Q000379:methods*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D009822:Oils, Volatile
keywords: Amomum villosum Lour; bioactive component; cell fishing; screening; solidification of floating organic drop liquid phase microextraction