title: [Cytotoxicity of T cells transduced with WT1 peptide-specific T-cell receptor gene against human lung cancer cells in vitro].
abstract: OBJECTIVE
  To investigate the cytotoxicity of normal CD8(+) T lymphocytes retrovirally transduced with WT1 peptide-specific T-cell receptor (TCR) genes against human lung cancer cells.
  
  
  METHODS
  HLA-A*2402-restricted and WT1 peptide-specific TCR-α/β genes were cloned from a cytotoxic T lymphocyte clone and inserted into a retroviral TCR expression vector. The cytotoxicity of normal peripheral CD8⁺ T cells transduced with the WT1-TCR genes against human lung cancer cells was evaluated using a standard ⁵¹Cr release assay.
  
  
  RESULTS
  The WT1-TCR gene-modified T cells recognized the peptide-pulsed target cells but not the non-pulsed cells. TCR-redirected CD8⁺ T cells lysed WT1-overexpressing human lung cancer cells in an HLA-A*2402-restricted manner, but did not kill normal cells positively expressing HLA-A*2402.
  
  
  CONCLUSIONS
  These data demonstrate the feasibility of adoptive immunotherapy with TCR-redirected T cell for the treatment of lung cancer.
authors.affiliation: Division of Cardiothoracic Surgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China. E-mail: anjun@mail.sysu.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: An
authors.forename: Song-Wang
authors.initials: SW
authors.lastname: Cai
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Junhang
authors.initials: J
authors.lastname: Zhang
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000166:cytology*; D045744:Cell Line, Tumor; D019672:Genes, T-Cell Receptor; D006801:Humans; D016219:Immunotherapy, Adoptive; D008175:Lung Neoplasms / Q000473:pathology*; D010455:Peptides; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D012190:Retroviridae; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology; D014161:Transduction, Genetic; D025721:WT1 Proteins / Q000235:genetics*
chemical_list: D010455:Peptides; D016693:Receptors, Antigen, T-Cell, alpha-beta; D025721:WT1 Proteins