title: Effects of MDM2 promoter polymorphisms on the development of cervical neoplasia in a Southeastern Brazilian population.
abstract: We investigated the importance of two adjacent functional polymorphisms in the Murine Double Minute 2 (MDM2) gene, SNP285 G > C and SNP309 T > G, for the development of cervical lesions in a Southeastern Brazilian population (293 cases and 184 controls). MDM2 genotyping was performed by PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) and/or DNA sequencing. MDM2 SNP309 has potential as a biomarker of cervical neoplasia in non-smokers, patients with family history of cancer, or those who had late sexual debut (>16 years). Besides, this polymorphism may help identify women at risk of developing severe cervical lesion at a young age (<30 years).
authors.affiliation: Department of Biochemistry, State University of Rio de Janeiro , Rio de Janeiro , Brazil .
authors.forename: Sandra Liliana
authors.initials: SL
authors.lastname: Vargas-Torres
authors.forename: Elyzabeth Avvad
authors.initials: EA
authors.lastname: Portari
authors.forename: Evandro Mendes
authors.initials: EM
authors.lastname: Klumb
authors.forename: Heloisa Carneiro da Rocha
authors.initials: HC
authors.lastname: Guillobel
authors.forename: Maria José de
authors.initials: MJ
authors.lastname: Camargo
authors.forename: Fábio Bastos
authors.initials: FB
authors.lastname: Russomano
authors.forename: Jacyara Maria Brito
authors.initials: JM
authors.lastname: Macedo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001938:Brazil / Q000453:epidemiology; D005006:Ethnicity; D005260:Female; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D051736:Proto-Oncogene Proteins c-mdm2 / Q000235:genetics*; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000235:genetics*; D055815:Young Adult
chemical_list: C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2
keywords: Age at onset; cervical neoplasia; genetic polymorphisms; susceptibility