title: [Determination of four phenolic endocrine disruptors in environmental water samples by high performance liquid chromatography-fluorescence detection using dispersive liquid-liquid microextraction coupled with derivatization].
abstract: To achieve accurate, fast and sensitive detection of phenolic endocrine disruptors in small volume of environmental water samples, a method of dispersive liquid-liquid microextraction (DLLME) coupled with fluorescent derivatization was developed for the determination of bisphenol A, nonylphenol, octylphenol and 4-tert-octylphenol in environmental water samples by high performance liquid chromatography-fluorescence detection (HPLC-FLD). The DLLME and derivatization conditions were investigated, and the optimized DLLME conditions for small volume of environmental water samples (pH 4.0) at room temperature were as follows: 70 microL chloroform as extraction solvent, 400 microL acetonitrile as dispersing solvent, vortex mixing for 3 min, and then high-speed centrifugation for 2 min. Using 2-[2-(7H-dibenzo [a, g] carbazol-7-yl)-ethoxy] ethyl chloroformate (DBCEC-Cl) as precolumn derivatization reagent, the stable derivatives of the four phenolic endocrine disruptors were obtained in pH 10.5 Na2CO3-NaHCO3 buffer/acetonitrile at 50 degrees C for 3 min, and then separated within 10 min by HPLC-FLD. The limits of detection (LODs) were in the range of 0.9-1.6 ng/L, and the limits of quantification (LOQs) were in the range of 3.8-7.1 ng/L. This method had perfect linearity, precision and recovery results, and showed obvious advantages and practicality comparing to the previously reported methods. It is a convenient and validated method for the routine analysis of phenolic endocrine disruptors in waste water of paper mill, lake water, domestic wastewater, tap water, etc.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.forename: Weimei
authors.initials: W
authors.lastname: Qi
authors.forename: Xian'en
authors.initials: X
authors.lastname: Zhao
authors.forename: Tao
authors.initials: T
authors.lastname: Lü
authors.forename: Xiya
authors.initials: X
authors.lastname: Wang
authors.forename: Longfang
authors.initials: L
authors.lastname: Zheng
authors.forename: Yehao
authors.initials: Y
authors.lastname: Yan
authors.forename: Jinmao
authors.initials: J
authors.lastname: You
mesh_terms: D001559:Benzhydryl Compounds; D002227:Carbazoles; D002725:Chloroform; D002851:Chromatography, High Pressure Liquid; D052244:Endocrine Disruptors / Q000032:analysis*; D004784:Environmental Monitoring; D005453:Fluorescence; D005618:Fresh Water; D057230:Limit of Detection; D059627:Liquid Phase Microextraction; D010636:Phenols; D012997:Solvents; D014867:Water / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D002227:Carbazoles; D002725:Chloroform; D052244:Endocrine Disruptors; D010636:Phenols; D012997:Solvents; D014867:Water; D014874:Water Pollutants, Chemical; C560574:2-(2-(7H-dibenzo(a,g)carbazol-7-yl)ethoxy)ethyl chloroformate; C474055:octylphenol; C025256:nonylphenol; C105260:4-tert-octylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds