title: Mechanism of DNA trapping in nanoporous structures during asymmetric pulsed-field electrophoresis.
abstract: We investigate the trapping mechanism of individual DNA molecules in ordered nanoporous structures generated by crystalline particle arrays. Two requisites for trapping are revealed by the dynamics of single trapped DNA, fully-stretched U/J shapes and hernia formation. The experimental results show there is a stronger possibility for hernias to lead the reorientation upon switching directions of the voltage at high field strengths, where trapping occurs. Fully stretched DNA has longer unhooking times than expected by a classic rope-on-pulley model. We propose a dielectrophoretic (DEP) force reduces the mobility of segments at the apex of the U or J, where field gradients are highest, based on simulations and observations of the trapping force dependence on field strength. A modified model for unhooking time is obtained after the DEP force is introduced. The new model explains the unhooking time data by predicting an infinite trapping time when the ratio of arm length differences (of the U or J) to molecule length Δx/L < β, where β is a DEP parameter that is found to strongly increase with electric field. The DNA polarizability calculated with the DEP model and experimental value of β is of the same magnitude of reported value. The results indicate the tension at the apex of U/J shape DNA is the primary reason for DNA trapping during pulsed field separation, instead of hernias.
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, AB, Canada. jed.harrison@ualberta.ca.
authors.forename: Ya
authors.initials: Y
authors.lastname: Zhou
authors.forename: Huiying
authors.initials: H
authors.lastname: Sheng
authors.forename: D Jed
authors.initials: DJ
authors.lastname: Harrison
mesh_terms: D004247:DNA / Q000737:chemistry*; D016521:Electrophoresis, Gel, Pulsed-Field; D049329:Nanostructures
chemical_list: D004247:DNA