title: Suppression of Eimeria tenella sporulation by disinfectants.
abstract: The disinfectant effects (DEs) of 10 types of chemicals, defined by their ability to destroy or inhibit oocysts and consequently prevent sporulation of Eimeria tenella field isolate, were evaluated in vitro. Correct species assignments and sample purities were confirmed by the singular internal transcribed spacer (ITS)-PCR analysis. A total of 18 treatments were performed, and the disinfection suppression levels were 75.9% for 39% benzene + 22% xylene (1:10 dilution), 85.5% for 30% cresol soup (1:1 dilution), and 91.7% for 99.9% acetic acid (1:2 dilution) group. The results indicate that acetic acid, cresol soup, and benzene+xylene are good candidates for suppression of E. tenella oocyst sporulation.
authors.affiliation: Laboratory of Veterinary Parasitology, College of Veterinary Medicine and Biosafety Research Center, Chonbuk National University, Jeonju 561-756, Korea.
authors.forename: Myung-Jo
authors.initials: MJ
authors.lastname: You
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004202:Disinfectants / Q000494:pharmacology*; D016786:Eimeria tenella / Q000187:drug effects* / Q000254:growth & development*; D008853:Microscopy; D008969:Molecular Sequence Data; D021261:Parasitic Sensitivity Tests; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D033761:Spores, Protozoan / Q000187:drug effects* / Q000254:growth & development*
chemical_list: D000981:Antiprotozoal Agents; D016054:DNA, Protozoan; D021903:DNA, Ribosomal Spacer; D004202:Disinfectants
keywords: Eimeria tenella; disinfectant; sporulation; suppression