title: Examination of galectin localization using confocal microscopy.
abstract: Confocal microscopy provides a unique modality to examine the expression and localization of biomolecules in a variety of settings. Using this technique, an image is acquired from the focal plane of the objective using focused laser light, making it possible to work within the resolution limit of the optical system. In addition, by acquiring multiple images from a variety of focal planes, stacked series of images can provide clear spatial localization of a probed structure or protein. We describe herein the immunofluorescence methods for galectin staining in frozen sections of tissue for galectin localization using confocal microscopy.
authors.affiliation: Faculty of Pharmaceutical Sciences of Ribeirão Preto, Department of Clinical, Toxicological and Bromatological Analysis, University of Sao Paulo, Ribeirão Preto-S, Brazil.
authors.forename: Daniel Giuliano
authors.initials: DG
authors.lastname: Cerri
authors.forename: Connie M
authors.initials: CM
authors.lastname: Arthur
authors.forename: Lílian Cataldi
authors.initials: LC
authors.lastname: Rodrigues
authors.forename: Marise Lopes
authors.initials: ML
authors.lastname: Fermino
authors.forename: Lenaldo Branco
authors.initials: LB
authors.lastname: Rocha
authors.forename: Sean R
authors.initials: SR
authors.lastname: Stowell
authors.forename: Marcelo Dias
authors.initials: MD
authors.lastname: Baruffi
mesh_terms: D005455:Fluorescent Antibody Technique; D037161:Galectins / Q000032:analysis / Q000378:metabolism*; D005898:Glass / Q000737:chemistry; D018613:Microscopy, Confocal / Q000379:methods*; D021381:Protein Transport
chemical_list: D037161:Galectins