title: [Dachengqi decoction reduces the serum levels of mast cell tryptase and inflammatory cytokines in rabbits with post-cardiac arrest syndrome].
abstract: OBJECTIVE
  To observe the effects of Dachengqi decoction on serum levels of mast cell tryptase, monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) in rabbits with post-cardiac arrest syndrome (PCAS).
  
  
  METHODS
  Thirty healthy male Japanese rabbits were randomly divided into three groups: sham-operation group, PCAS model group and Dachengqi decoction treatment group. The model of PCAS was established by asphyxia-induced cardiac arrest. Fifteen minutes after return of spontaneous circulation, Dachengqi decoction [15 g/(kg.d), bid] was given by intra-gastric administration in Dachengqi decoction treatment group. The indicators of organ function were evaluated 24, 48 and 72 hours after cardiac arrest. The serum levels of mast cell tryptase, MCP-1 and IL-8 were determined by ELISA.
  
  
  RESULTS
  Dachengqi decoction alleviated the dysfunction significantly in heart, brain, liver and kidney. Compared with the sham group, the serum levels of mast cell tryptase, MCP-1 and IL-8 increased significantly in PCAS group (P<0.01). Compared with the PCAS group, the serum levels of mast cell tryptase (at 6 hours), MCP-1 (at 6, 24 and 48 hours) and IL-8 (at 6 and 24 hours) decreased significantly in Dachengqi decoction treatment group (P<0.05 or P<0.01).
  
  
  CONCLUSIONS
  Dachengqi decoction can reduce the serum levels of mast cell tryptase, MCP-1 and IL-8 in rabbits with post-cardiac arrest syndrome.
authors.affiliation: Department of Emergency Medicine, Taihe Hospital, Department of Immunology, Hubei Medical University, Shiyan 442000, China.
authors.forename: Jingning
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Emergency Medicine, Taihe Hospital, Shiyan 442000, China.
authors.forename: Min
authors.initials: M
authors.lastname: Xiao
authors.affiliation: Department of Emergency Medicine, Taihe Hospital, Shiyan 442000, China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Emergency Medicine, Taihe Hospital, Shiyan 442000, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Emergency Medicine, Taihe Hospital, Shiyan 442000, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Liang
authors.affiliation: Department of Emergency Medicine, Taihe Hospital, Shiyan 442000, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chai
authors.affiliation: Department of Emergency Medicine, Taihe Hospital, Shiyan 442000, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Luo
mesh_terms: D000818:Animals; D018932:Chemokine CCL2 / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006323:Heart Arrest / Q000097:blood / Q000503:physiopathology / Q000517:prevention & control*; D016209:Interleukin-8 / Q000097:blood*; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D011817:Rabbits; D011897:Random Allocation; D013577:Syndrome; D016896:Treatment Outcome; D053802:Tryptases / Q000097:blood*
chemical_list: D018932:Chemokine CCL2; D016209:Interleukin-8; D010936:Plant Extracts; C477169:dachengqi decoction; D053802:Tryptases