title: Comparison of antiviral activity of lambda-interferons against HIV replication in macrophages.
abstract: Lambda-interferons (IFN-λs) have been demonstrated as having the ability to inhibit HIV replication in macrophages. However, specific differences in signaling transduction and anti-HIV activity in macrophages between different IFN-λs are unclear. Here, we showed that although all 3 members of (IFN-λ1, λ2, and λ3) IFN-λ family induced the expression of a number of genes of janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway in monocyte-derived macrophages, IFN-λ1 or IFN-λ3 induced higher levels of antiviral IFN-stimulated genes (ISGs) expression than did IFN-λ2. In addition, IFN-λ1 or IFN-λ3 induced higher levels of several pattern recognition receptors (PPRs) than did IFN-λ2. Incubation of IFN-λs with HIV-infected macrophages showed that IFN-λ1 or IFN-λ3 is more potent in anti-HIV activity than IFN-λ2. We also showed that IFN-λ treatment before HIV infection was more potent in HIV inhibition than that after HIV infection. Further investigations showed that the inductions of ISGs and PPRs expression by IFN-λs were largely compromised by HIV infection. These findings provide further experimental evidence that IFN-λs have therapeutic potential in treatment of HIV infection.
authors.affiliation: 1 Department of Infectious Diseases, Shanghai Children's Hospital, Shanghai Jiao Tong University , Shanghai, China .
authors.forename: Yizhong
authors.initials: Y
authors.lastname: Wang
authors.forename: Jieliang
authors.initials: J
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.forename: Wenzhe
authors.initials: W
authors.lastname: Ho
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology; D002478:Cells, Cultured; D006678:HIV / Q000502:physiology*; D015658:HIV Infections / Q000276:immunology / Q000628:therapy*; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D007372:Interferons; D007378:Interleukins / Q000494:pharmacology*; D053612:Janus Kinases / Q000378:metabolism; D008264:Macrophages / Q000276:immunology* / Q000821:virology; D020218:Response Elements / Q000187:drug effects; D050791:STAT Transcription Factors / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects; D000095022:Interferon Lambda
chemical_list: D000998:Antiviral Agents; D007372:Interferons; D007378:Interleukins; D053612:Janus Kinases; D050791:STAT Transcription Factors; C000624353:interferon-lambda, human; D000095022:Interferon Lambda