title: Phenotypic and genetic features of enteropathogenic Escherichia coli isolates from diarrheal children in the Ribeirão Preto metropolitan area, São Paulo State, Brazil.
abstract: This study was designed to characterize a collection of 60 enteropathogenic Escherichia coli (EPEC) isolates from diarrheic feces of patients in the Ribeirão Preto metropolitan area regarding different phenotypic and molecular features. We examined antibiotic resistance profiles, occurrence of virulence factors-encoding genes, intimin subtypes and the correlation of serotypes among typical (tEPEC) and atypical (aEPEC) EPEC isolates. The results demonstrated that atypical EPEC was more heterogeneous than typical EPEC concerning the characteristics investigated and 45.2% do not belong to classical EPEC serogroups. Intimin subtype β was the most frequent among the EPEC isolates (46.7%), being detected in both tEPEC and aEPEC. The majority of aEPEC isolates presented localized adherence-like (LAL) pattern to HEp-2 cells, although aEPEC isolates displaying diffuse adherence (DA) or non-adherent were also detected. High prevalence of antimicrobial resistance was found for ampicillin, cephalothin, sulfonamide and tetracycline. In general, tEPEC isolates were more resistant to the antimicrobials tested than aEPEC isolates.
authors.affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.
authors.forename: André
authors.initials: A
authors.lastname: Pitondo-Silva
authors.forename: Gerson
authors.initials: G
authors.lastname: Nakazato
authors.forename: Juliana P
authors.initials: JP
authors.lastname: Falcão
authors.forename: Kinue
authors.initials: K
authors.lastname: Irino
authors.forename: Roberto
authors.initials: R
authors.lastname: Martinez
authors.forename: Ana Lúcia C
authors.initials: AL
authors.lastname: Darini
authors.forename: Rodrigo Tavanelli
authors.initials: RT
authors.lastname: Hernandes
mesh_terms: D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000235:genetics; D001938:Brazil / Q000453:epidemiology; D002460:Cell Line; D002512:Cephalothin / Q000494:pharmacology; D002675:Child, Preschool; D003967:Diarrhea / Q000382:microbiology; D024901:Drug Resistance, Multiple, Bacterial; D054308:Enteropathogenic Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000188:drug therapy; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008826:Microbial Sensitivity Tests; D012703:Serotyping; D013449:Sulfonamides / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology; D037521:Virulence Factors / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D013449:Sulfonamides; D037521:Virulence Factors; D000667:Ampicillin; D013752:Tetracycline; D002512:Cephalothin
keywords: Enteropathogenic E. coli; antimicrobial resistance; intimin; serotypes; typical and atypical EPEC