title: Uptake and subcellular distributions of cadmium and selenium in transplanted aquatic insect larvae.
abstract: We transplanted larvae of the phantom midge Chaoborus punctipennis from a lake having lower concentrations of Cd and Se (Lake Dasserat) to a more contaminated lake (Lake Dufault) located near a metal smelter in Rouyn-Noranda, Quebec. Transplanted individuals were held in mesh mesocosms for up to 16 days where they were fed with indigenous contaminated zooplankton. Larval Cd and Se burdens increased over time, and came to equal those measured in indigenous C. punctipennis from contaminated Lake Dufault. Larval Se burdens increased steadily, whereas those of Cd showed an initial lag phase that we explain by a change in the efficiency with which this insect assimilated Cd from its prey. We measured Cd and Se in subcellular fractions and found that larvae sequestered the majority (60%) of the incoming Cd in a detoxified fraction containing metal-binding proteins, whereas a minority of this nonessential metal was in sensitive fractions (20%). In contrast, a much higher proportion of the essential element Se (40%) was apportioned to metabolically active sensitive fractions. Larvae took up equimolar quantities of these elements over the course of the experiment. Likewise, Cd and Se concentrations in wild larvae were equimolar, which suggests that they are exposed to equimolar bioavailable concentrations of these elements in our study lakes.
authors.affiliation: Institut national de la recherche scientifique, Centre Eau Terre Environnement (INRS-ETE), 490 de la Couronne, Québec, Québec, Canada , G1K 9A9.
authors.forename: Maikel
authors.initials: M
authors.lastname: Rosabal
authors.forename: Dominic E
authors.initials: DE
authors.lastname: Ponton
authors.forename: Peter G C
authors.initials: PG
authors.lastname: Campbell
authors.forename: Landis
authors.initials: L
authors.lastname: Hare
mesh_terms: D000818:Animals; D059001:Aquatic Organisms / Q000378:metabolism*; D002104:Cadmium / Q000378:metabolism*; D002683:Chironomidae; D060106:Lakes / Q000737:chemistry; D007814:Larva / Q000378:metabolism; D011235:Predatory Behavior; D011792:Quebec; D012643:Selenium / Q000378:metabolism*; D013347:Subcellular Fractions / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors; D014131:Trace Elements / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000378:metabolism
chemical_list: D014131:Trace Elements; D014874:Water Pollutants, Chemical; D002104:Cadmium; D012643:Selenium