title: Expression of histone H3 lysine 4 methylation and its demethylases in the developing mouse testis.
abstract: Histone H3 lysine 4 methylation (H3K4me) is an epigenetic modification associated with gene activation and is dynamically regulated by histone methylases and demethylases. To date, the expression patterns of H3K4me and its demethylases in the developing testis remain unclear. The present study was designed to detect the expression of H3K4me1/2/3 and its demethylases LSD1, RBP2 and SMCX in 21-, 40- and 60-day-old mouse testes by using immunohistochemistry, quantitative real-time polymerase chain reaction (PCR) and Western blot. The immunohistochemical results demonstrated that the expression patterns of the same protein were similar in testes at different ages and that the positive staining cell types were mainly Leydig cells, type A and B spermatogonia, leptotene spermatocytes and spermatids for H3K4me1/2/3, Leydig cells, type A spermatogonia, zygotene and pachytene spermatocytes, spermatids, and Sertoli cells for LSD1 and type A and B spermatogonia for RBP2. Immunostaining for SMCX was not detected in testes. Quantitative real-time PCR and Western blot showed that the amounts of LSD1, RPB2 and SMCX mRNA and protein were age-dependent, were significantly reduced with increasing age and exhibited a negative correlation with the protein levels of H3K4me1/2/3. Thus, H3K4me, which is modified by its demethylases, probably plays a role in male spermatogenesis and testis development.
authors.affiliation: Department of Biology, School of Medicine, Jiangsu University, No. 301, Xuefu Road, Zhenjiang, 212013, Jiangsu Province, People's Republic of China.
authors.forename: Liuping
authors.initials: L
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Yaoqian
authors.initials: Y
authors.lastname: Pan
authors.forename: Jie
authors.initials: J
authors.lastname: Jin
authors.forename: Jianrong
authors.initials: J
authors.lastname: Sang
authors.forename: Pan
authors.initials: P
authors.lastname: Huang
authors.forename: Genbao
authors.initials: G
authors.lastname: Shao
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015971:Gene Expression Regulation, Enzymologic; D056466:Histone Demethylases / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D007150:Immunohistochemistry; D008239:Lysine / Q000378:metabolism*; D008297:Male; D008745:Methylation; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013737:Testis / Q000166:cytology / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D006657:Histones; D012333:RNA, Messenger; D056466:Histone Demethylases; D008239:Lysine