title: Morphological and morphometric analyses of crushed sciatic nerves after application of a purified protein from natural latex and hyaluronic acid hydrogel.
abstract: Hyaluronic acid hydrogels (HAHs) have been used as a carrier of substances and factors in the repair of nervous tissue. Natural latex protein (Hevea brasiliensis, F1) has shown positive effects in treating various types of tissues, including peripheral nerves. This study evaluated the F1 associated with a HAH in a controlled crush injury (axonotmesis) of the sciatic nerve in Wistar rats. The samples were photomicrographed for morphometric and quantitative analyzes using ImageJ 1.47k software (NIH, Bethesda, MD). Morphological, quantitative (myelin area/nerve area ratio and capillary density) and morphometric (minimum nerve fiber diameter, G-Ratio) data revealed an improvement in the recovery of the sciatic nerve with the application of HAH and the combination of HAH and F1 after 4 and 8 weeks of nerve injury. The most efficacious results were observed with the combination of both substances, F1 and HAH, revealing the regenerative capacity of this new biomaterial, which was hardly tested on nerve tissue.
authors.affiliation: School of Dentistry of Ribeirao Preto, University of Sao Paulo , Ribeirao Preto , Brazil .
authors.forename: Vanessa Cristina Pereira
authors.initials: VC
authors.lastname: Barreiros
authors.forename: Fernando José
authors.initials: FJ
authors.lastname: Dias
authors.forename: Mamie Mizusaki
authors.initials: MM
authors.lastname: Iyomasa
authors.forename: Joaquim
authors.initials: J
authors.lastname: Coutinho-Netto
authors.forename: Luiz Gustavo
authors.initials: LG
authors.lastname: de Sousa
authors.forename: Valéria Paula Sassoli
authors.initials: VP
authors.lastname: Fazan
authors.forename: Ricardo de Souza
authors.initials: Rde S
authors.lastname: Antunes
authors.forename: Ii-Sei
authors.initials: IS
authors.lastname: Watanabe
authors.forename: João Paulo Mardegan
authors.initials: JP
authors.lastname: Issa
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D028482:Hevea / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D007840:Latex / Q000737:chemistry*; D009186:Myelin Sheath / Q000737:chemistry; D009409:Nerve Crush; D009416:Nerve Regeneration; D010525:Peripheral Nerves / Q000473:pathology; D010936:Plant Extracts / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000293:injuries* / Q000473:pathology*; D020426:Sciatic Neuropathy; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D007840:Latex; D010936:Plant Extracts; D006820:Hyaluronic Acid
keywords: Hyaluronic acid hydrogel – HAH; morphology and morphometry; natural latex protein – F1; peripheral nerve injury