title: Assessing the metabolic impact of nitrogen availability using a compartmentalized maize leaf genome-scale model.
abstract: Maize (Zea mays) is an important C4 plant due to its widespread use as a cereal and energy crop. A second-generation genome-scale metabolic model for the maize leaf was created to capture C4 carbon fixation and investigate nitrogen (N) assimilation by modeling the interactions between the bundle sheath and mesophyll cells. The model contains gene-protein-reaction relationships, elemental and charge-balanced reactions, and incorporates experimental evidence pertaining to the biomass composition, compartmentalization, and flux constraints. Condition-specific biomass descriptions were introduced that account for amino acids, fatty acids, soluble sugars, proteins, chlorophyll, lignocellulose, and nucleic acids as experimentally measured biomass constituents. Compartmentalization of the model is based on proteomic/transcriptomic data and literature evidence. With the incorporation of information from the MetaCrop and MaizeCyc databases, this updated model spans 5,824 genes, 8,525 reactions, and 9,153 metabolites, an increase of approximately 4 times the size of the earlier iRS1563 model. Transcriptomic and proteomic data have also been used to introduce regulatory constraints in the model to simulate an N-limited condition and mutants deficient in glutamine synthetase, gln1-3 and gln1-4. Model-predicted results achieved 90% accuracy when comparing the wild type grown under an N-complete condition with the wild type grown under an N-deficient condition.
authors.affiliation: Departments of Chemical Engineering (M.S., R.S., C.D.M.) and Bioinformatics and Genomics, Huck Institutes of the Life Sciences (A.K.), Pennsylvania State University, University Park, Pennsylvania 16802;Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, Centre de Versailles-Grignon, Unité Mixte de Recherche 1318 Institut National de la Recherche Agronomique-Agro-ParisTech, Equipe de Recherce Labellisée, Centre National de la Recherche Scientifique 3559, F-78026 Versailles cedex, France (N.A., L.G., G.C., M.M., Z.L., G.M., B.H.); andLancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom (P.J.L.).
authors.forename: Margaret
authors.initials: M
authors.lastname: Simons
authors.affiliation: Departments of Chemical Engineering (M.S., R.S., C.D.M.) and Bioinformatics and Genomics, Huck Institutes of the Life Sciences (A.K.), Pennsylvania State University, University Park, Pennsylvania 16802;Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, Centre de Versailles-Grignon, Unité Mixte de Recherche 1318 Institut National de la Recherche Agronomique-Agro-ParisTech, Equipe de Recherce Labellisée, Centre National de la Recherche Scientifique 3559, F-78026 Versailles cedex, France (N.A., L.G., G.C., M.M., Z.L., G.M., B.H.); andLancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom (P.J.L.).
authors.forename: Rajib
authors.initials: R
authors.lastname: Saha
authors.affiliation: Departments of Chemical Engineering (M.S., R.S., C.D.M.) and Bioinformatics and Genomics, Huck Institutes of the Life Sciences (A.K.), Pennsylvania State University, University Park, Pennsylvania 16802;Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, Centre de Versailles-Grignon, Unité Mixte de Recherche 1318 Institut National de la Recherche Agronomique-Agro-ParisTech, Equipe de Recherce Labellisée, Centre National de la Recherche Scientifique 3559, F-78026 Versailles cedex, France (N.A., L.G., G.C., M.M., Z.L., G.M., B.H.); andLancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom (P.J.L.).
authors.forename: Nardjis
authors.initials: N
authors.lastname: Amiour
authors.affiliation: Departments of Chemical Engineering (M.S., R.S., C.D.M.) and Bioinformatics and Genomics, Huck Institutes of the Life Sciences (A.K.), Pennsylvania State University, University Park, Pennsylvania 16802;Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, Centre de Versailles-Grignon, Unité Mixte de Recherche 1318 Institut National de la Recherche Agronomique-Agro-ParisTech, Equipe de Recherce Labellisée, Centre National de la Recherche Scientifique 3559, F-78026 Versailles cedex, France (N.A., L.G., G.C., M.M., Z.L., G.M., B.H.); andLancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom (P.J.L.).
authors.forename: Akhil
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Departments of Chemical Engineering (M.S., R.S., C.D.M.) and Bioinformatics and Genomics, Huck Institutes of the Life Sciences (A.K.), Pennsylvania State University, University Park, Pennsylvania 16802;Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, Centre de Versailles-Grignon, Unité Mixte de Recherche 1318 Institut National de la Recherche Agronomique-Agro-ParisTech, Equipe de Recherce Labellisée, Centre National de la Recherche Scientifique 3559, F-78026 Versailles cedex, France (N.A., L.G., G.C., M.M., Z.L., G.M., B.H.); andLancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom (P.J.L.).
authors.forename: Lenaïg
authors.initials: L
authors.lastname: Guillard
authors.affiliation: Departments of Chemical Engineering (M.S., R.S., C.D.M.) and Bioinformatics and Genomics, Huck Institutes of the Life Sciences (A.K.), Pennsylvania State University, University Park, Pennsylvania 16802;Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, Centre de Versailles-Grignon, Unité Mixte de Recherche 1318 Institut National de la Recherche Agronomique-Agro-ParisTech, Equipe de Recherce Labellisée, Centre National de la Recherche Scientifique 3559, F-78026 Versailles cedex, France (N.A., L.G., G.C., M.M., Z.L., G.M., B.H.); andLancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom (P.J.L.).
authors.forename: Gilles
authors.initials: G
authors.lastname: Clément
authors.affiliation: Departments of Chemical Engineering (M.S., R.S., C.D.M.) and Bioinformatics and Genomics, Huck Institutes of the Life Sciences (A.K.), Pennsylvania State University, University Park, Pennsylvania 16802;Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, Centre de Versailles-Grignon, Unité Mixte de Recherche 1318 Institut National de la Recherche Agronomique-Agro-ParisTech, Equipe de Recherce Labellisée, Centre National de la Recherche Scientifique 3559, F-78026 Versailles cedex, France (N.A., L.G., G.C., M.M., Z.L., G.M., B.H.); andLancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom (P.J.L.).
authors.forename: Martine
authors.initials: M
authors.lastname: Miquel
authors.affiliation: Departments of Chemical Engineering (M.S., R.S., C.D.M.) and Bioinformatics and Genomics, Huck Institutes of the Life Sciences (A.K.), Pennsylvania State University, University Park, Pennsylvania 16802;Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, Centre de Versailles-Grignon, Unité Mixte de Recherche 1318 Institut National de la Recherche Agronomique-Agro-ParisTech, Equipe de Recherce Labellisée, Centre National de la Recherche Scientifique 3559, F-78026 Versailles cedex, France (N.A., L.G., G.C., M.M., Z.L., G.M., B.H.); andLancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom (P.J.L.).
authors.forename: Zhenni
authors.initials: Z
authors.lastname: Li
authors.affiliation: Departments of Chemical Engineering (M.S., R.S., C.D.M.) and Bioinformatics and Genomics, Huck Institutes of the Life Sciences (A.K.), Pennsylvania State University, University Park, Pennsylvania 16802;Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, Centre de Versailles-Grignon, Unité Mixte de Recherche 1318 Institut National de la Recherche Agronomique-Agro-ParisTech, Equipe de Recherce Labellisée, Centre National de la Recherche Scientifique 3559, F-78026 Versailles cedex, France (N.A., L.G., G.C., M.M., Z.L., G.M., B.H.); andLancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom (P.J.L.).
authors.forename: Gregory
authors.initials: G
authors.lastname: Mouille
authors.affiliation: Departments of Chemical Engineering (M.S., R.S., C.D.M.) and Bioinformatics and Genomics, Huck Institutes of the Life Sciences (A.K.), Pennsylvania State University, University Park, Pennsylvania 16802;Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, Centre de Versailles-Grignon, Unité Mixte de Recherche 1318 Institut National de la Recherche Agronomique-Agro-ParisTech, Equipe de Recherce Labellisée, Centre National de la Recherche Scientifique 3559, F-78026 Versailles cedex, France (N.A., L.G., G.C., M.M., Z.L., G.M., B.H.); andLancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom (P.J.L.).
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Lea
authors.affiliation: Departments of Chemical Engineering (M.S., R.S., C.D.M.) and Bioinformatics and Genomics, Huck Institutes of the Life Sciences (A.K.), Pennsylvania State University, University Park, Pennsylvania 16802;Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, Centre de Versailles-Grignon, Unité Mixte de Recherche 1318 Institut National de la Recherche Agronomique-Agro-ParisTech, Equipe de Recherce Labellisée, Centre National de la Recherche Scientifique 3559, F-78026 Versailles cedex, France (N.A., L.G., G.C., M.M., Z.L., G.M., B.H.); andLancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom (P.J.L.).
authors.forename: Bertrand
authors.initials: B
authors.lastname: Hirel
authors.affiliation: Departments of Chemical Engineering (M.S., R.S., C.D.M.) and Bioinformatics and Genomics, Huck Institutes of the Life Sciences (A.K.), Pennsylvania State University, University Park, Pennsylvania 16802;Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, Centre de Versailles-Grignon, Unité Mixte de Recherche 1318 Institut National de la Recherche Agronomique-Agro-ParisTech, Equipe de Recherce Labellisée, Centre National de la Recherche Scientifique 3559, F-78026 Versailles cedex, France (N.A., L.G., G.C., M.M., Z.L., G.M., B.H.); andLancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom (P.J.L.) costas@psu.edu.
authors.forename: Costas D
authors.initials: CD
authors.lastname: Maranas
mesh_terms: D001682:Biological Availability; D018533:Biomass; D020869:Gene Expression Profiling; D018745:Genome, Plant; D055442:Metabolome; D008954:Models, Biological; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism* / Q000493:pharmacokinetics; D018515:Plant Leaves / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism*
chemical_list: D020543:Proteome; D009584:Nitrogen