title: Deep sequencing of the scallop Chlamys farreri transcriptome response to tetrabromobisphenol A (TBBPA) stress.
abstract: Tetrabromobisphenol-A (TBBPA) is currently the most widely used brominated flame retardant (BFR) and has been proven to have a very high toxicity to aquatic organisms including bivalves. However, molecular responses to TBBPA in bivalve remain largely unknown. Novel high-throughput deep sequencing technology has been a powerful tool for looking at molecular responses to toxicological stressors in organisms. Using Illumina's digital gene expression (DGE) system, we investigated TBBPA-induced transcriptome response in the digestive gland tissue of scallop Chlamys farreri. In total, 173 and 266 genes were identified as significantly up- or down-regulated, respectively. Functional analysis based on gene ontology (GO) classification system and Kyoto Encyclopedia of Genes and Genomes (KEGG) database revealed that TBBPA significantly altered the expression of genes involved in stress response, detoxification, antioxidation, and innate immunity which were extensively discussed. In particular, evidence for the endocrine disrupting effect of TBBPA on bivalve was first obtained in this study. Quantitative real-time PCR was performed to ascertain the mRNA expression of several genes identified by the DGE analysis. The results of this study may serve as a basis for future research on molecular mechanism of toxic effects of TBBPA on marine bivalves.
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Fengxiao
authors.initials: F
authors.lastname: Hu
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China. Electronic address: panlq@ouc.edu.cn.
authors.forename: Luqing
authors.initials: L
authors.lastname: Pan
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Yuefeng
authors.initials: Y
authors.lastname: Cai
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Tong
authors.initials: T
authors.lastname: Liu
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Jin
mesh_terms: D000818:Animals; D052244:Endocrine Disruptors / Q000633:toxicity*; D005411:Flame Retardants / Q000633:toxicity*; D063990:Gene Ontology; D059014:High-Throughput Nucleotide Sequencing; D049897:Pectinidae / Q000235:genetics*; D011075:Polybrominated Biphenyls / Q000633:toxicity*; D060888:Real-Time Polymerase Chain Reaction; D013312:Stress, Physiological / Q000187:drug effects / Q000502:physiology*; D059467:Transcriptome
chemical_list: D052244:Endocrine Disruptors; D005411:Flame Retardants; D011075:Polybrominated Biphenyls; C020806:tetrabromobisphenol A
keywords: Chlamys farreri; Differential expression; Digital gene expression; Illumina sequencing; Tetrabromobisphenol A