title: The protective role of vitamin E on the testicular tissue in rats exposed to sodium arsenite during the prenatal stage till sex maturity: A stereological analysis.
abstract: BACKGROUND
  Vitamin E is an effective antioxidant, protecting cells against oxidative stress.
  
  
  OBJECTIVE
  In this investigation the protective effect of vitamin E on the testis during development and spermatogenesis in rats exposed to sodium arsenite was evaluated.
  
  
  METHODS
  Pregnant Wistar rats were divided into 4 groups (n=8) control, sodium arsenite (8 mg/kg/day), sodium arsenite+vitamin E (100 mg/kg/day) and vitamin E. Treatment was carried out from day seven of pregnancy till 90 days. Finally the right testis was stereologically studied. The obtained data was analyzed using one way ANOVA and Tukey's test and the means difference was considered significant at p<0.05.
  
  
  RESULTS
  The weight and volume of testis, volume of seminiferous tubules and its diameter, volume of interstitial tissue, height of germinal epithelium and the total number of types A and B spermatogonia, spermatocyte, spermatid and sertoli cells reduced significantly in sodium arsenite group compared to the control. Co-administration of vitamin E and sodium arsenite compensated the adverse effects of sodium arsenite on the above parameters.
  
  
  CONCLUSIONS
  We concluded co-treatment of rats with sodium arsenite and vitamin E could prevent the adverse effects of sodium arsenite exposure on the testicular tissue during the prenatal stage till sex maturity.
authors.affiliation: Department of Biology, Faculty of Sciences, Arak University, Arak, Iran.
authors.forename: Malek
authors.initials: M
authors.lastname: Soleimani Mehranjani
authors.affiliation: Department of Biology, Faculty of Sciences, Arak University, Arak, Iran.
authors.forename: Rezvan
authors.initials: R
authors.lastname: Taefi
keywords: Rat; Sodium arsenite; Stereology; Testis; Vitamin E