title: Effect of ELF-EMF Exposure on Human Neuroblastoma Cell Line: a Proteomics Analysis.
abstract: BACKGROUND
  Extremely low frequency electromagnetic fields (ELF-EMF) have been common in daily life all over the world. They have produced by power lines and electrical appliances, but higher levels of them have raised a lot of concerns about their carcinogenesis. Both epidemiological and laboratory studies have suggested that EMFs might increase cancer incidence, including acute childhood leukemia, brain and breast cancer.
  
  
  METHODS
  In the present study, SH-SY5Y human neuroblastoma cell line has exposed to 2mT, 50 Hz magnetic field for 3 h. Next, effect of this exposure on protein expression including over-expression or under-expression has assessed by proteomics.
  
  
  RESULTS
  Bioinformatics and statistical analysis using progenesis same spot software on the obtained 2D electrophoresis has shown that expression of 189 proteins in exposed group has changed relative to control. Besides, PCA analysis has verified results of clustering, and has shown that protein data has clustered according to experimental conditions.
  
  
  CONCLUSIONS
  The results of this study have shown that ELF-EMF changes cell morphology via altering protein expression, but more profound studies have needed to determine the kind of proteins altered.
authors.affiliation: Dept. of Medical Physics, Semnan University of Medical Sciences, Semnan, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Hasanzadeh
authors.affiliation: Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Rezaie-Tavirani
authors.affiliation: Dept. of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Samaneh Sadat
authors.initials: SS
authors.lastname: Seyyedi
authors.affiliation: Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Hakimeh
authors.initials: H
authors.lastname: Zali
authors.affiliation: Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Heydari Keshel
authors.affiliation: Dept. of Medical Physics, Semnan University of Medical Sciences, Semnan, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Jadidi
authors.affiliation: Dept. of Anatomy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Abedelahi
keywords: Electromagnetic fields; Neuroblastoma; Proteomics