title: Silencing of CDC42 inhibits neuroblastoma cell proliferation and transformation.
abstract: Cell division cycle 42 (CDC42), a small GTPase of the Rho-subfamily, regulates diverse cellular functions including proliferation, cytoskeletal rearrangement and even promotes malignant transformation. Here, we found that increased expression of CDC42 correlated with undifferentiated neuroblastoma as compared to a more benign phenotype. CDC42 inhibition decreased cell growth and soft agar colony formation, and increased cell death in BE(2)-C and BE(2)-M17 cell lines, but not in SK-N-AS. In addition, silencing of CDC42 decreased expression of N-myc in BE(2)-C and BE(2)-M17 cells. Our findings suggest that CDC42 may play a role in the regulation of aggressive neuroblastoma behavior.
authors.affiliation: Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
authors.forename: Sora
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
authors.forename: Brian T
authors.initials: BT
authors.lastname: Craig
authors.affiliation: Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
authors.forename: Carmelle V
authors.initials: CV
authors.lastname: Romain
authors.affiliation: Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Qiao
authors.affiliation: Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA. Electronic address: dai.chung@vanderbilt.edu.
authors.forename: Dai H
authors.initials: DH
authors.lastname: Chung
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D000071447:N-Myc Proto-Oncogene Protein; D009447:Neuroblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D020764:cdc42 GTP-Binding Protein / Q000037:antagonists & inhibitors* / Q000235:genetics* / Q000378:metabolism
chemical_list: C459801:MYCN protein, human; D000071447:N-Myc Proto-Oncogene Protein; D009687:Nuclear Proteins; D015513:Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D020764:cdc42 GTP-Binding Protein
keywords: AKT2; CDC42; N-myc; Neuroblastoma; Transformation; Twist