title: Polymorphism of heat shock protein 70-2 and enterocutaneous fistula in Chinese population.
abstract: OBJECTIVE
  To investigate whether the heat shock protein 70-2 (HSP70-2) polymorphism is associated with enterocutaneous fistulas in a Chinese population.
  
  
  METHODS
  This study included 131 patients with enterocutaneous/enteroatmospheric fistulas. Patients with inflammatory bowel disease or other autoimmune diseases were excluded from this study. All patients with enterocutaneous/enteroatmospheric fistulas were followed up for three months to observe disease recurrence. In addition, a total of 140 healthy controls were also recruited from the Jinling Hospital, matched according to the sex and age of the patient population. Genomic DNA was extracted from peripheral blood from each participant. The HSP70-2 restriction fragment length polymorphism related to the polymorphic PstI site at position 1267 was characterized by polymerase chain reaction (PCR). First PCR amplification was carried out, and then PCR products were digested with PstI restriction enzyme. The DNA lacking the polymorphic PstI site within HSP70-2 generates a product of 1117 bp in size (allele A), whereas the HSP70-2 PstI polymorphism produces two fragments of 936 bp and 181 bp in size (allele B).
  
  
  RESULTS
  The frequency of the HSP70-2 PstI polymorphism did not differ between patients and controls; however, the A allele was more predominant in patients with enterocutaneous fistulas than in controls (60.7% vs 51.4%, P = 0.038, OR = 1.425, 95%CI: 1.019-1.994). Sixty-one patients were cured by a definitive operation, drainage operation, or percutaneous drainage while 52 patients were cured by nonsurgical treatment. There was no significant difference in the frequency of the HSP70-2 PstI polymorphism between the patients who had surgery compared to those who did not (P = 0.437, OR = 1.237, 95%CI: 0.723-2.117). Moreover, 11 patients refused any treatment for economic reasons or tumor burden, and 7 patients with enterocutaneous fistulas (5.8%) died during the follow-up period. However, there was no significant difference in the frequency of the HSP70-2 PstI polymorphism between the patients who survived compared to those who died (P = 0.403, OR = 0.604, 95%CI: 0.184-1.986).
  
  
  CONCLUSIONS
  The A allele of the HSP70-2 PstI polymorphism was associated with enterocutaneous fistulas in this Chinese population.
authors.affiliation: Jun Chen, Jian-An Ren, Guo-Sheng Gu, Ge-Fei Wang, Xiu-Wen Wu, Yun-Zhao Zhao, Jie-Shou Li, Department of Surgery, the Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, Jiangsu Province, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: Jun Chen, Jian-An Ren, Guo-Sheng Gu, Ge-Fei Wang, Xiu-Wen Wu, Yun-Zhao Zhao, Jie-Shou Li, Department of Surgery, the Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, Jiangsu Province, China.
authors.forename: Jian-An
authors.initials: JA
authors.lastname: Ren
authors.affiliation: Jun Chen, Jian-An Ren, Guo-Sheng Gu, Ge-Fei Wang, Xiu-Wen Wu, Yun-Zhao Zhao, Jie-Shou Li, Department of Surgery, the Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, Jiangsu Province, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Han
authors.affiliation: Jun Chen, Jian-An Ren, Guo-Sheng Gu, Ge-Fei Wang, Xiu-Wen Wu, Yun-Zhao Zhao, Jie-Shou Li, Department of Surgery, the Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, Jiangsu Province, China.
authors.forename: Guo-Sheng
authors.initials: GS
authors.lastname: Gu
authors.affiliation: Jun Chen, Jian-An Ren, Guo-Sheng Gu, Ge-Fei Wang, Xiu-Wen Wu, Yun-Zhao Zhao, Jie-Shou Li, Department of Surgery, the Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, Jiangsu Province, China.
authors.forename: Ge-Fei
authors.initials: GF
authors.lastname: Wang
authors.affiliation: Jun Chen, Jian-An Ren, Guo-Sheng Gu, Ge-Fei Wang, Xiu-Wen Wu, Yun-Zhao Zhao, Jie-Shou Li, Department of Surgery, the Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, Jiangsu Province, China.
authors.forename: Xiu-Wen
authors.initials: XW
authors.lastname: Wu
authors.affiliation: Jun Chen, Jian-An Ren, Guo-Sheng Gu, Ge-Fei Wang, Xiu-Wen Wu, Yun-Zhao Zhao, Jie-Shou Li, Department of Surgery, the Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, Jiangsu Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Jun Chen, Jian-An Ren, Guo-Sheng Gu, Ge-Fei Wang, Xiu-Wen Wu, Yun-Zhao Zhao, Jie-Shou Li, Department of Surgery, the Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, Jiangsu Province, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Hu
authors.affiliation: Jun Chen, Jian-An Ren, Guo-Sheng Gu, Ge-Fei Wang, Xiu-Wen Wu, Yun-Zhao Zhao, Jie-Shou Li, Department of Surgery, the Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, Jiangsu Province, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Jun Chen, Jian-An Ren, Guo-Sheng Gu, Ge-Fei Wang, Xiu-Wen Wu, Yun-Zhao Zhao, Jie-Shou Li, Department of Surgery, the Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, Jiangsu Province, China.
authors.forename: Yun-Zhao
authors.initials: YZ
authors.lastname: Zhao
authors.affiliation: Jun Chen, Jian-An Ren, Guo-Sheng Gu, Ge-Fei Wang, Xiu-Wen Wu, Yun-Zhao Zhao, Jie-Shou Li, Department of Surgery, the Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, Jiangsu Province, China.
authors.forename: Jie-Shou
authors.initials: JS
authors.lastname: Li
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002681:China / Q000453:epidemiology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D007412:Intestinal Fistula / Q000175:diagnosis / Q000208:ethnology / Q000235:genetics* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D012008:Recurrence; D012307:Risk Factors; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D018840:HSP70 Heat-Shock Proteins; C525621:HSPA1B protein, human
keywords: Enterocutaneous fistulas; HSP70-2; Heat shock protein; Single nucleotide polymorphisms