title: Rapid neutral lipid accumulation of the alkali-resistant oleaginous Monoraphidium dybowskii LB50 by NaCl induction.
abstract: NaCl is an effective inducer of lipid accumulation in freshwater microalgae, but little is known on whether the enhanced lipid components are desired. To address this issue, Monoraphidium dybowskii LB50 from a freshwater habitat was selected, cultivated, and subjected to NaCl induction at different scales outdoors. Results showed that the optimal salt concentration reduced glycolipid (GL) content, as well as enhanced neutral lipid (NL) and phospholipid (PL) contents. Moreover, GL was preferentially converted to NL at 20gL(-1) NaCl. Total lipid and NL contents respectively increased to 41.7% and 17.48% in 1d. The highest NL productivity was also achieved at both the 5L (24.13mgL(-1)d(-1)) and 140L (13.05mgL(-1)d(-1), 3.43gm(-2)d(-1)) scales. These results suggest that NL accumulated effectively and rapidly at different scales, indicating that this strategy has broad application prospects for the scale-up cultivation of oily algae.
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Haijian
authors.initials: H
authors.lastname: Yang
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Qiaoning
authors.initials: Q
authors.lastname: He
authors.affiliation: SINOPEC Research Institute of Petroleum Processing, Beijing 100083, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Rong
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Xia
authors.affiliation: Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. Electronic address: cxhu@ihb.ac.cn.
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Hu
mesh_terms: D000388:Air; D000468:Alkalies / Q000494:pharmacology*; D018533:Biomass; D008027:Light; D050356:Lipid Metabolism / Q000187:drug effects* / Q000528:radiation effects; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D058433:Photobioreactors / Q000382:microbiology; D012965:Sodium Chloride / Q000494:pharmacology*; D013696:Temperature
chemical_list: D000468:Alkalies; D008055:Lipids; D012965:Sodium Chloride
keywords: Glycolipids; Monoraphidium dybowskii LB50; NaCl induction; Neutral lipid productivity; Phospholipids