title: Diagnostic value of a Rec-ELISA using Toxoplasma gondii recombinant SporoSAG, BAG1, and GRA1 proteins in murine models infected orally with tissue cysts and oocysts.
abstract: Toxoplasma gondii causes congenital toxoplasmosis in newborns resulting with fetal anomalies. Determining the initiation time of infection is very important for pregnant women and current serological assays have drawbacks in distinguishing the recently acute toxoplasmosis. Diagnosis of recently acute infection may be improved by using stage specific antigens in serological assays. In the present study, the diagnostic value of sporozoite specific SporoSAG, bradyzoite specific BAG1 proteins and GRA1 protein expressed by all forms of the parasite have been evaluated ELISA using sera systematically collected from mice administered orally with tissue cyst and oocysts. The anti-SporoSAG IgM antibodies in sera obtained from mice infected with oocysts peaked significantly at days 1, 10, and 15 (P<0.01). The anti-BAG1 IgM antibodies in sera obtained from mice infected with tissue cysts peaked significantly at days 15, 40, and 120 (P<0.05). The anti-GRA1 IgM antibodies in sera obtained from mice infected with oocysts peaked significantly at days 2, 10, and 40 (P<0.01). The anti-GRA1 IgM antibodies in sera obtained from mice infected with tissue cysts peaked significantly only at day 40 (P<0.05). The anti-SporoSAG, anti-BAG1, and anti-GRA1 IgG titers of mice showed significant increases at day 40 (P<0.05) and decrement started for only anti-GRA1 IgG at day 120. The presence of anti-SporoSAG IgM and IgG antibodies can be interpreted as recently acute infection between days 10-40 because IgM decreases at day 40. Similarly, presence of anti-BAG1 IgM and absence of IgG can be evaluated as a recently acute infection that occurred 40 days before because IgG peaks at day 40. A peak in anti-GRA1 antibody level at first testing and reduction in consecutive sample can be considered as an infection approximately around day 40 or prior. Overall, recombinant SporoSAG, BAG1 and GRA1 proteins can be accepted as valuable diagnostic markers of recently acute toxoplasmosis.
authors.affiliation: Department of Parasitology, Ege University Medical School, Bornova/İzmir, Turkey.
authors.forename: Mert
authors.initials: M
authors.lastname: Döşkaya
authors.affiliation: Department of Parasitology, Ege University Medical School, Bornova/İzmir, Turkey.
authors.forename: Ayşe
authors.initials: A
authors.lastname: Caner
authors.affiliation: Department of Parasitology, Ege University Medical School, Bornova/İzmir, Turkey; Department of Molecular Biology, Ege University Faculty of Sciences, Bornova/İzmir, Turkey.
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Can
authors.affiliation: Department of Bioengineering, Ege University Faculty of Engineering, Bornova/İzmir, Turkey.
authors.forename: Sultan
authors.initials: S
authors.lastname: Gülçe İz
authors.affiliation: Department of Parasitology, Ege University Medical School, Bornova/İzmir, Turkey; Department of Bioengineering, Ege University Faculty of Engineering, Bornova/İzmir, Turkey.
authors.forename: Yaprak
authors.initials: Y
authors.lastname: Gedik
authors.affiliation: Department of Parasitology, Ege University Medical School, Bornova/İzmir, Turkey.
authors.forename: Aysu Değirmenci
authors.initials: AD
authors.lastname: Döşkaya
authors.affiliation: Department of Dermatology, University of California Irvine, Irvine, California, United States of America.
authors.forename: Mina
authors.initials: M
authors.lastname: Kalantari-Dehaghi
authors.affiliation: Department of Parasitology, Ege University Medical School, Bornova/İzmir, Turkey.
authors.forename: Yüksel
authors.initials: Y
authors.lastname: Gürüz
mesh_terms: D000208:Acute Disease; D000284:Administration, Oral; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000235:genetics / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D051379:Mice; D033661:Oocysts / Q000737:chemistry / Q000276:immunology*; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D014122:Toxoplasma / Q000737:chemistry / Q000276:immunology; D014124:Toxoplasmosis, Animal / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; C106179:BAG1 protein, Toxoplasma; C412913:GRA1 antigen; D006360:Heat-Shock Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C047168:SAG1 antigen, Toxoplasma