title: Multichannel MRI labeling of mammalian cells by switchable nanocarriers for hyperpolarized xenon.
abstract: We demonstrate a concept for multichannel MRI cell-labeling using encapsulated laser-polarized xenon. Conceptually different Xe trapping properties of two nanocarriers, namely macrocyclic cages as individual hosts or compartmentalization into nanodroplets, ensure a large chemical shift separation for Xe bound in either of the carriers even after cellular internalization. Two differently labeled mammalian cell populations were imaged by frequency selective saturation transfer resulting in a switchable "two-color" xenon-MRI contrast at micro- to nanomolar Xe carrier concentrations.
authors.affiliation: ERC Project BiosensorImaging, Leibniz-Institut für Molekulare Pharmakologie (FMP) , 13125 Berlin, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Klippel
authors.forename: Christian
authors.initials: C
authors.lastname: Freund
authors.forename: Leif
authors.initials: L
authors.lastname: Schröder
keywords: CEST; MRI; Multiplexing; cell-labeling; hyperpolarization; xenon