title: Developmental toxicity and DNA damage to zebrafish induced by perfluorooctane sulfonate in the presence of ZnO nanoparticles.
abstract: Perfluorooctane sulfonate (PFOS) and ZnO nanoparticles (ZnO-NPs) are frequently detected in the environment, but few studies have assessed their joint toxicity. In this study, the acute toxicity and chronic toxicity to zebrafish (Danio rerio) induced by PFOS in the presence of ZnO-NPs were investigated, including developmental toxicity and DNA damage. The embryos were exposed to PFOS (only) (0.4, 0.8, and 1.6 mg/L) and PFOS plus ZnO-NPs (0.4 + 50, 0.8 + 50, and 1.6 + 50 mg/L) solutions to evaluate mortality (96 h), body length (96 h), hatch rate (72 h), heart rate (48 h),and malformation rate (96 h). The results revealed that the co-treatment could cause more severe developmental toxicity compared with the control and single-treatments, and the toxic effects generally increased in a dose-response manner. In addition, adult zebrafish were exposed to single and mixed solutions of PFOS and ZnO-NPs (at the concentrations mentioned above) for 30 days. DNA damage to zebrafish was evaluated by the comet assay and micronucleus test. We found that the PFOS single-treatment at all doses (0.4, 0.8, and 1.6 mg/L) could strongly induce DNA damage to peripheral blood cells and that ZnO-NPs could aggravate the formation of DNA damage in co-treatments. Histological examination of liver, testicle, and ovary showed that the presence of ZnO-NPs (50 mg/L) could also cause more serious histological damage to adult zebrafish than PFOS alone. As a result, the synergistic effects played an important role during joint exposure. Our observations provide a basic understanding of the joint toxicity of PFOS and ZnO-NPs to aquatic organisms.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Weihai, 150090, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Du
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Weihai, 150090, China.
authors.forename: Shutao
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Weihai, 150090, China.|School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, 264209, China.
authors.forename: Hong
authors.initials: H
authors.lastname: You
authors.affiliation: Heilongjiang Research Academy of Environmental Sciences, Harbin, 150056, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Jiang
authors.affiliation: Life science and Environmental Science Research Center, Harbin Institute of Commerce, Harbin, 150028, China.
authors.forename: Changlu
authors.initials: C
authors.lastname: Zhuang
authors.affiliation: Pathology Department, Fourth Clinical Hospital of Harbin Medical University, Harbin, 150001, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhang
mesh_terms: D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000818:Animals; D001773:Blood Cells / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects*; D004347:Drug Interactions; D004625:Embryo, Nonmammalian; D047108:Embryonic Development / Q000187:drug effects* / Q000235:genetics; D005260:Female; D005466:Fluorocarbons / Q000633:toxicity*; D008099:Liver / Q000187:drug effects; D053768:Metal Nanoparticles / Q000633:toxicity*; D015027:Zebrafish / Q000097:blood / Q000196:embryology / Q000235:genetics; D015034:Zinc Oxide / Q000633:toxicity*
chemical_list: D017738:Alkanesulfonic Acids; D005466:Fluorocarbons; C076994:perfluorooctane sulfonic acid; D015034:Zinc Oxide
keywords: DNA damage; ZnO nanoparticles; comet assay; micronucleus; perfluorooctane sulfonate