title: Prophylactic effect of the oral administration of transgenic rice seeds containing altered peptide ligands of type II collagen on rheumatoid arthritis.
abstract: Rheumatoid arthritis is an autoimmune disease associated with the recognition of self proteins secluded in arthritic joints. We generated transgenic rice seeds expressing three types of altered peptide ligands (APL) and the T cell epitope of type II collagen (CII256-271). When these transgenic rice and non-transgenic rice seeds were orally administrated to DBA/1 J mice once a day for 14 days, followed by immunization with CII, the clinical score of collagen-induced arthritis (CIA) was reduced and inflammation and erosion in the joints were prevented in mice fed APL7 transgenic rice only. IL-10 production against the CII antigen significantly increased in the splenocytes and iLN of CIA mice immunized with the CII antigen, whereas IFN-γ, IL-17, and IL-2 levels were not altered. These results suggest that IL-10-mediated immune suppression is involved in the prophylactic effects caused by transgenic rice expressing APL7.
authors.affiliation: a Faculty of Medicine, Department of Internal Medicine , University of Tsukuba , Tsukuba , Japan.
authors.forename: Mana
authors.initials: M
authors.lastname: Iizuka
authors.forename: Yuhya
authors.initials: Y
authors.lastname: Wakasa
authors.forename: Hiroto
authors.initials: H
authors.lastname: Tsuboi
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Asashima
authors.forename: Tomoya
authors.initials: T
authors.lastname: Hirota
authors.forename: Yuya
authors.initials: Y
authors.lastname: Kondo
authors.forename: Isao
authors.initials: I
authors.lastname: Matsumoto
authors.forename: Takayuki
authors.initials: T
authors.lastname: Sumida
authors.forename: Fumio
authors.initials: F
authors.lastname: Takaiwa
mesh_terms: D000284:Administration, Oral; D000595:Amino Acid Sequence; D000818:Animals; D001169:Arthritis, Experimental / Q000276:immunology / Q000517:prevention & control*; D024043:Collagen Type II / Q000276:immunology*; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D010455:Peptides / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics*; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology
chemical_list: D024043:Collagen Type II; D008024:Ligands; D010455:Peptides
keywords: altered peptide ligands; autoreactive T cells; rheumatoid arthritis; transgenic rice; type II collagen