title: Imaging of cardiac and renal perfusion in a rat model with 13N-NH 3 micro-PET.
abstract: Cardiac dysfunction leads to decreased organ perfusion. We aimed to measure cardiac and renal perfusion simultaneously with the use of (13)N-NH3-microPET in different rat models. Ten male Wistar rats underwent sham surgery (n = 5) or permanent coronary artery ligation to induce myocardial infarction (MI, n = 5). Eleven weeks later (13)N-NH3-microPET scan was performed to study the cardiac and renal perfusion. Cardiac perfusion was significantly reduced in MI group, directly correlated with ejection fraction and inversely correlated with MI size (r = 0.89; p < 0.001 and r = -0.86; p < 0.001 respectively). Renal perfusion showed a notional 17 % non-significant reduction in MI group when compared to sham (3.44 ± 0.40 vs. 4.12 ± 0.48 ml/g/min). There was a trend towards greater reduction of perfusion in cortical than medullar region. Cortex perfusion was negatively correlated with histological changes. (13)N-NH3-microPET may be a potential tool for evaluation of cardiac and renal functional and perfusion changes in presence of cardiac dysfunction in rat models.
authors.affiliation: Department of Nuclear Medicine and Molecular Imaging, University Medical Center Groningen, University of Groningen, Hanzeplein 1, P.O. Box 30001, 9700 RB, Groningen, The Netherlands.
authors.forename: Luis E
authors.initials: LE
authors.lastname: Juárez-Orozco
authors.forename: Mariusz K
authors.initials: MK
authors.lastname: Szymanski
authors.forename: Hans L
authors.initials: HL
authors.lastname: Hillege
authors.forename: Silvana
authors.initials: S
authors.lastname: Kruizinga
authors.forename: Walter
authors.initials: W
authors.lastname: Noordzij
authors.forename: Michel
authors.initials: M
authors.lastname: Koole
authors.forename: René A
authors.initials: RA
authors.lastname: Tio
authors.forename: Erick
authors.initials: E
authors.lastname: Alexanderson
authors.forename: Rudi A J O
authors.initials: RA
authors.lastname: Dierckx
authors.forename: Riemer H J A
authors.initials: RH
authors.lastname: Slart
mesh_terms: D000641:Ammonia; D000818:Animals; D003326:Coronary Circulation; D004195:Disease Models, Animal; D008297:Male; D009203:Myocardial Infarction / Q000000981:diagnostic imaging* / Q000503:physiopathology; D055414:Myocardial Perfusion Imaging / Q000379:methods*; D009206:Myocardium / Q000473:pathology; D049268:Positron-Emission Tomography; D011237:Predictive Value of Tests; D019275:Radiopharmaceuticals; D017208:Rats, Wistar; D012079:Renal Circulation; D013318:Stroke Volume
chemical_list: D019275:Radiopharmaceuticals; D000641:Ammonia