title: Evaluation of liver regeneration with use of diet supplemented with L-arginine.
abstract: OBJECTIVE
  To compare controlled liver regeneration in rats submitted to 60% hepatic resection having L-arginine supplemented diet, based on weight changes of the regenerated liver, laboratory parameters of liver function and pathological findings.
  
  
  METHODS
  Thirty-six rats were divided into two groups, control and L- arginine. The first received standard chow and saline solution by gavage. The second had supplementation with L- arginine. Animals were killed on postoperative period at 24h, 72h and seven days. For analysis of liver regeneration was used Kwon formula for weight, laboratory tests and mitosis.
  
  
  RESULTS
  Weight, showed no benefit with L- arginine supplementation; however, intergroup comparison in the first 24h observed positive effect on supplementation (p=0.008). Alkaline phosphatase was increased in arginine group (p<0.04). The number of mitoses showed no difference between the two groups; however, in the first 24 hours, the supplemented group had higher number of mitoses within the groups (p=0.03).
  
  
  CONCLUSIONS
  Supplementation with L-arginine did not show benefits in liver regeneration; however, supplemented group in the first 24 hours showed benefits over 72 hours and seven days of the evaluation by weight gain and number of mitosis.
authors.affiliation: FEPAR, Curitiba, PR, Brazil.
authors.forename: Widlani Sousa
authors.initials: WS
authors.lastname: Montenegro
authors.affiliation: Federal University of Parana, Curitiba, PR, Brazil.
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Malafaia
authors.affiliation: Federal University of Parana, Curitiba, PR, Brazil.
authors.forename: Paulo Afonso Nunes
authors.initials: PA
authors.lastname: Nassif
authors.affiliation: UFPR, Curitiba, PR, Brazil.
authors.forename: Luciane Bugmann
authors.initials: LB
authors.lastname: Moreira
authors.affiliation: FEPAR, Curitiba, PR, Brazil.
authors.forename: Manoel Alberto
authors.initials: MA
authors.lastname: Prestes
authors.affiliation: FEPAR, Curitiba, PR, Brazil.
authors.forename: Márcio Hiroaki
authors.initials: MH
authors.lastname: Kume
authors.affiliation: FEPAR, Curitiba, PR, Brazil.
authors.forename: Leandro Bressianini
authors.initials: LB
authors.lastname: Jurkonis
authors.affiliation: FEPAR, Curitiba, PR, Brazil.
authors.forename: Igor Furlan
authors.initials: IF
authors.lastname: Cella
mesh_terms: D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D019587:Dietary Supplements; D006498:Hepatectomy; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D008115:Liver Regeneration / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008938:Mitosis / Q000187:drug effects / Q000502:physiology; D009929:Organ Size; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D001120:Arginine