title: Immunohistological Description of Nongestational Ovarian Choriocarcinoma in Two Female Mice With Conditional Loss of Trp53 Driven by the Tie2 Promoter.
abstract: Nongestational ovarian choriocarcinoma (NGCO) is a tumor of germ cell origin seldom described in nonhuman species. Few spontaneous cases are reported in macaques and mice, with the B6C3F1 strain overrepresented. This report describes 2 cases of ovarian choriocarcinoma in nulliparous female mice with conditional loss of Trp53 under the Tie2 promoter. The mouse line was maintained on a mixed genetic background including Crl: CD1(ICR) and 129X1/SvJ strains. In both cases, affected ovary was partially replaced by blood-filled lacunae lined by neoplastic trophoblast-like giant cells. Immunohistochemically, neoplastic cells expressed folate-binding protein and prolactin and were invariably negative for p53. To the authors' knowledge, this is the first report characterizing this entity in a genetically engineered mouse (GEM) line. Considering that germ cells (the cell population from which NGCO originates) constitutively express Tie2 receptor, it can be speculated that Tie2-driven deletion of Trp53 may have played a role in the development of these tumors.
authors.affiliation: Department of Veterinary Science and Public Health, Veterinary Medicine, University of Milan, Via Celoria, Milan, Italy Mouse & Animal Pathology Lab, Fondazione Filarete, Viale Ortles, Milan, Italy vittoria.castiglioni@unimi.it.
authors.forename: V
authors.initials: V
authors.lastname: Castiglioni
authors.affiliation: VIB-Department of Molecular Biomedical Research, Vascular Cell Biology Unit, Ghent University, Ghent, Belgium VIB-Molecular Signal Transduction in Inflammation Unit, Inflammation Research Center; VIB-Ghent University, Ghent, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Farhang Ghahremani
authors.affiliation: VIB-Department of Molecular Biomedical Research, Vascular Cell Biology Unit, Ghent University, Ghent, Belgium VIB-Molecular Signal Transduction in Inflammation Unit, Inflammation Research Center; VIB-Ghent University, Ghent, Belgium Department for Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: Goossens
authors.affiliation: Department of Veterinary Science and Public Health, Veterinary Medicine, University of Milan, Via Celoria, Milan, Italy Mouse & Animal Pathology Lab, Fondazione Filarete, Viale Ortles, Milan, Italy.
authors.forename: M
authors.initials: M
authors.lastname: De Maglie
authors.affiliation: Merck Serono RBM S.p.A., Istituto di Ricerche Biomediche, Colleretto Giacosa, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Ardizzone
authors.affiliation: VIB-Department of Molecular Biomedical Research, Vascular Cell Biology Unit, Ghent University, Ghent, Belgium.
authors.forename: J J
authors.initials: JJ
authors.lastname: Haigh
authors.affiliation: Department of Veterinary Science and Public Health, Veterinary Medicine, University of Milan, Via Celoria, Milan, Italy Mouse & Animal Pathology Lab, Fondazione Filarete, Viale Ortles, Milan, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Radaelli
mesh_terms: D000818:Animals; D002822:Choriocarcinoma / Q000473:pathology / Q000662:veterinary*; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D009373:Neoplasms, Germ Cell and Embryonal / Q000473:pathology / Q000662:veterinary*; D010051:Ovarian Neoplasms / Q000473:pathology / Q000662:veterinary*; D011247:Pregnancy; D042787:Receptor, TIE-2 / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D016159:Tumor Suppressor Protein p53; D042787:Receptor, TIE-2; C079086:Tek protein, mouse
keywords: Trp53; choriocarcinoma; genetically engineered mouse; ovary