title: Examination of the role of galectins in cell migration and re-epithelialization of wounds.
abstract: Re-epithelialization is a crucial step for wound healing. As galectins play important roles in re-epithelialization, we describe here protocols for in vivo, ex vivo and in vitro examination of the role of galectins in cell migration and in re-epithelialization of wounds. For in vivo models, mouse corneas are wounded by a variety of techniques and the rate of re-epithelialization is quantified. For ex vivo organ culture models, mouse corneas are wounded in situ, the eyes are enucleated, the eyeballs are cultured in the presence or absence of galectins and the rate of re-epithelialization is quantified. For cell cultured-based in vitro assays, we examine formation of lamellipodia and activation of focal adhesion kinase in various epithelial cells.
authors.affiliation: New England Eye Center and Department of Ophthalmology, Tufts University, 136 Harrison Avenue, Boston, MA, 02111, USA.
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Cao
authors.forename: Chandrassegar
authors.initials: C
authors.lastname: Saravanan
authors.forename: Wei-Sheng
authors.initials: WS
authors.lastname: Chen
authors.forename: Noorjahan
authors.initials: N
authors.lastname: Panjwani
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D003315:Cornea / Q000166:cytology; D004789:Enzyme Activation; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D037161:Galectins / Q000378:metabolism*; D006801:Humans; D051379:Mice; D009924:Organ Culture Techniques; D011554:Pseudopodia / Q000378:metabolism; D062326:Re-Epithelialization; D014945:Wound Healing
chemical_list: D037161:Galectins; D051416:Focal Adhesion Protein-Tyrosine Kinases