title: Constructing a high-density linkage map for Gossypium hirsutum × Gossypium barbadense and identifying QTLs for lint percentage.
abstract: To introgress the good fiber quality and yield from Gossypium barbadense into a commercial Upland cotton variety, a high-density simple sequence repeat (SSR) genetic linkage map was developed from a BC1 F1 population of Gossypium hirsutum × Gossypium barbadense. The map comprised 2,292 loci and covered 5115.16 centiMorgan (cM) of the cotton AD genome, with an average marker interval of 2.23 cM. Of the marker order for 1,577 common loci on this new map, 90.36% agrees well with the marker order on the D genome sequence genetic map. Compared with five published high-density SSR genetic maps, 53.14% of marker loci were newly discovered in this map. Twenty-six quantitative trait loci (QTLs) for lint percentage (LP) were identified on nine chromosomes. Nine stable or common QTLs could be used for marker-assisted selection. Fifty percent of the QTLs were from G. barbadense and increased LP by 1.07%-2.41%. These results indicated that the map could be used for screening chromosome substitution segments from G. barbadense in the Upland cotton background, identifying QTLs or genes from G. barbadense, and further developing the gene pyramiding effect for improving fiber yield and quality.
authors.affiliation: State Key Laboratory of Cotton Biology, Key Laboratory of Biological and Genetic Breeding of Cotton, the Ministry of Agriculture, Institute of Cotton Research, the Chinese Academy of Agricultural Sciences, Anyang, 455000, China.
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Shi
authors.forename: Wentan
authors.initials: W
authors.lastname: Li
authors.forename: Aiguo
authors.initials: A
authors.lastname: Li
authors.forename: Ruihua
authors.initials: R
authors.lastname: Ge
authors.forename: Baocai
authors.initials: B
authors.lastname: Zhang
authors.forename: Junzhi
authors.initials: J
authors.lastname: Li
authors.forename: Guangping
authors.initials: G
authors.lastname: Liu
authors.forename: Junwen
authors.initials: J
authors.lastname: Li
authors.forename: Aiying
authors.initials: A
authors.lastname: Liu
authors.forename: Haihong
authors.initials: H
authors.lastname: Shang
authors.forename: Juwu
authors.initials: J
authors.lastname: Gong
authors.forename: Wankui
authors.initials: W
authors.lastname: Gong
authors.forename: Zemao
authors.initials: Z
authors.lastname: Yang
authors.forename: Feiyü
authors.initials: F
authors.lastname: Tang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Liu
authors.forename: Weiping
authors.initials: W
authors.lastname: Zhu
authors.forename: Jianxiong
authors.initials: J
authors.lastname: Jiang
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Yu
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Tingting
authors.initials: T
authors.lastname: Chen
authors.forename: Kunbo
authors.initials: K
authors.lastname: Wang
authors.forename: Zhengsheng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Youlu
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D000483:Alleles; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D003433:Crosses, Genetic; D005787:Gene Frequency / Q000235:genetics; D008040:Genetic Linkage; D056426:Genetic Loci; D005819:Genetic Markers; D018745:Genome, Plant; D005838:Genotype; D003368:Gossypium / Q000235:genetics*; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics; D011110:Polymorphism, Genetic; D011123:Polyploidy; D040641:Quantitative Trait Loci / Q000235:genetics*; D013784:Textiles
chemical_list: D005819:Genetic Markers
keywords: Cotton (Gossypium spp.); Gossypium barbadense; genetic linkage map; lint percentage; simple sequence repeat