title: [Effect of FarR on transcriptional levels of arginine biosynthetic genes in Corynebacterium crenatum AS 1.542 and its relationship with ArgR].
abstract: OBJECTIVE
  The FarR protein was involved in the regulation of arginine biosynthetic pathway in corynebacterium, but the regulation mechanism of FarR protein and its relationship with the negative regulator ArgR have never been reported. In this work, we constructed two deletion mutants: C. crenatum delta farR and C. crenatum delta argR delta farR, and investigated the FarR function and its relationship with ArgR through the determination of transcriptional levels of arginine biosynthetic genes in four strains, including C. crenatum delta argR constructed in previous work.
  
  
  METHODS
  We used marker-less knockout technology to construct C. crenatum delta farR and C. crenatum delta argR delta farR, and compared the transcriptional levels of the arginine biosynthetic genes in three mutant strains with those of the wild type strain using real-time fluorescence quantitative PCR.
  
  
  RESULTS
  The results of RT-qPCR indicate that, in the absence of ArgR, FarR acted as a positive regulator. When farR gene was knockout alone, the transcriptional levels of arginine biosynthetic genes appeared up-regulated, down-regulated or no influence.
  
  
  CONCLUSIONS
  FarR and ArgR are involved together in the regulation of arginine biosynthetic pathway of C. crenatum.
authors.forename: Huifeng
authors.initials: H
authors.lastname: Shao
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Lv
authors.initials: L
authors.lastname: Wang
authors.forename: Haitao
authors.initials: H
authors.lastname: Jiao
authors.forename: Li
authors.initials: L
authors.lastname: Tang
authors.forename: Xuelan
authors.initials: X
authors.lastname: Chen
mesh_terms: D001120:Arginine / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003352:Corynebacterium / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C087018:ArgR protein, Bacteria; D001426:Bacterial Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D001120:Arginine