title: Potential interplay between NFκB and PPARγ in human dermal microvascular endothelial cells cultured in low magnesium.
abstract: Dermal microvascular endothelial cells contribute to cutaneous inflammation by secreting proinflammatory cytokines and chemokines. We show here that low extracellular magnesium stimulates the secretion of interleukin 8 and monocyte chemoattractant protein-1 in dermal microvascular endothelial cells. This secretory pattern might result from interplay between NFκB, the master regulator of inflammation, and PPARγ, a transcription factor that has emerged as an inhibitor of inflammation. Indeed, both NFκB and PPARγ are activated in dermal microvascular endothelial cells cultured in low magnesium-containing medium. In conclusion, we hypothesize that PPARγ and NFκB might contribute to the response of these cells to low Mg.
authors.affiliation: Dipartimento di Scienze Biomediche e Cliniche L. Sacco, Università di Milano, Milano, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Castiglioni
authors.affiliation: Dipartimento di Scienze Biomediche e Cliniche L. Sacco, Università di Milano, Milano, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Cazzaniga
authors.affiliation: Dipartimento di Scienze Biomediche e Cliniche L. Sacco, Università di Milano, Milano, Italy.
authors.forename: Jeanette A M
authors.initials: JA
authors.lastname: Maier
mesh_terms: D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D008274:Magnesium / Q000008:administration & dosage*; D008833:Microcirculation / Q000187:drug effects / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism*; D047495:PPAR gamma / Q000378:metabolism*
chemical_list: D016328:NF-kappa B; D047495:PPAR gamma; D008274:Magnesium
keywords: NFκB; PPARγ; chemokines; magnesium; microvascular endothelial cells