title: A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for the detection of bifenthrin in a chemical soil barrier.
abstract: A sensitive monoclonal antibody-based enzyme monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) was developed to detect bifenthrin in a chemical soil barrier for termite control. LBc ((2-methyl[1,1-biphenyl]-3-methoxy) carbonyl propionic acid) was conjugated to bovine serum albumin (BSA) for producing monoclonal antibody. After optimization, the IC50 and limit of detection (LOD, IC10) were 0.05 mg L(-1) and 0.004 mg L(-1), respectively. The sensitivity was improved 40-fold compared to polyclonal antibody-based ELISA reported earlier. No cross-reactivity was measured for the other analogues such as cyhalothrin, cyhalothric acid, cypermethrin, deltamethrin, fenpropathrin, fenvalerate, ethofenprox and tetramethrin except 2-methyl-3-biphenylmethyanol. Spiked recoveries were between 83.5% and 104.7% for the detection of bifenthrin in loess, red soil and black soil. All the relative standard deviations (RSDs) were less than or equal to 15.0%. Moreover, the ELISA for authentic samples showed reliability and high correlation with gas chromatography. The developed ELISA is an alternative tool for simple, sensitive and accurate monitoring of bifenthrin in chemical soil barriers.
authors.affiliation: College of Plant Protection (State and Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University, Nanjing 210095, China; Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing, China.
authors.forename: Xiude
authors.initials: X
authors.lastname: Hua
authors.affiliation: College of Plant Protection (State and Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University, Nanjing 210095, China; Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Plant Protection (State and Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University, Nanjing 210095, China; Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yin
authors.affiliation: Wuxi Termite Control Center, Wuxi 214071, China.
authors.forename: Yazhong
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Wuxi Termite Control Center, Wuxi 214071, China.
authors.forename: Qiujun
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Wuxi Termite Control Center, Wuxi 214071, China.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Wuxi Termite Control Center, Wuxi 214071, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Plant Protection (State and Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University, Nanjing 210095, China; Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Shi
authors.affiliation: College of Plant Protection (State and Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University, Nanjing 210095, China; Institute of Plant Protection, Jiangsu Academy of Agricultural Science, Nanjing 210014, China.
authors.forename: Fengquan
authors.initials: F
authors.lastname: Liu
authors.affiliation: College of Plant Protection (State and Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University, Nanjing 210095, China; Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing, China. Electronic address: wangmha@njau.edu.cn.
authors.forename: Minghua
authors.initials: M
authors.lastname: Wang
mesh_terms: D004784:Environmental Monitoring / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007306:Insecticides / Q000032:analysis*; D057230:Limit of Detection; D011722:Pyrethrins / Q000032:analysis*; D015203:Reproducibility of Results; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants / Q000032:analysis*
chemical_list: D007306:Insecticides; D011722:Pyrethrins; D012987:Soil; D012989:Soil Pollutants; C099952:bifenthrin
keywords: Bifenthrin; Chemical soil barrier; Enzyme-linked immunosorbent assay; Monoclonal antibody