title: Global ablation of the mouse Rab11a gene impairs early embryogenesis and matrix metalloproteinase secretion.
abstract: Rab11a has been conceived as a prominent regulatory component of the recycling endosome, which acts as a nexus in the endo- and exocytotic networks. The precise in vivo role of Rab11a in mouse embryonic development is unknown. We globally ablated Rab11a and examined the phenotypic and molecular outcomes in Rab11a(null) blastocysts and mouse embryonic fibroblasts. Using multiple trafficking assays and complementation analyses, we determined, among multiple important membrane-associated and soluble cargos, the critical contribution of Rab11a vesicular traffic to the secretion of multiple soluble MMPs. Rab11a(null) embryos were able to properly form normal blastocysts but died at peri-implantation stages. Our data suggest that Rab11a critically controls mouse blastocyst development and soluble matrix metalloproteinase secretion.
authors.affiliation: Department of Biological Sciences, Rutgers University, Newark, New Jersey 07102.
authors.forename: Shiyan
authors.initials: S
authors.lastname: Yu
authors.affiliation: Transgenic Core Facility, Rutgers New Jersey Medical School, Newark, New Jersey 07103.
authors.forename: Ghassan
authors.initials: G
authors.lastname: Yehia
authors.affiliation: Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, and.
authors.forename: Juanfei
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Biological Sciences, Rutgers University, Newark, New Jersey 07102.
authors.forename: Ewa
authors.initials: E
authors.lastname: Stypulkowski
authors.affiliation: Department of Biological Sciences, Rutgers University, Newark, New Jersey 07102.
authors.forename: Ryotaro
authors.initials: R
authors.lastname: Sakamori
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724.
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Department of Biological Sciences, Rutgers University, Newark, New Jersey 07102.
authors.forename: Berenice
authors.initials: B
authors.lastname: Hernandez-Enriquez
authors.affiliation: Department of Biological Sciences, Rutgers University, Newark, New Jersey 07102.
authors.forename: Tracy S
authors.initials: TS
authors.lastname: Tran
authors.affiliation: Department of Biological Sciences, Rutgers University, Newark, New Jersey 07102.
authors.forename: Edward M
authors.initials: EM
authors.lastname: Bonder
authors.affiliation: Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, and.
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Biological Sciences, Rutgers University, Newark, New Jersey 07102,. Electronic address: ngao@andromeda.rutgers.edu.
authors.forename: Nan
authors.initials: N
authors.lastname: Gao
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000483:Alleles; D000818:Animals; D001755:Blastocyst / Q000166:cytology; D047108:Embryonic Development; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D018507:Gene Expression Regulation, Developmental; D016678:Genome; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D011247:Pregnancy; D011270:Pregnancy, Animal; D014168:Transferrin / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000502:physiology*; D000099193:rab11 GTP-Binding Proteins
chemical_list: D000469:Alkaline Phosphatase; D020778:Matrix Metalloproteinase 2; D020783:Matrix Metalloproteinase 7; D020782:Matrix Metalloproteinases; D014168:Transferrin; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C522362:Mmp2 protein, mouse
keywords: Embryo; Matrix Metalloproteinase (MMP); Mouse Genetics; Rab; Rab11; Rab11a; Trafficking; rab11