title: Novel fermentation process strengthening strategy for production of propionic acid and vitamin B12 by Propionibacterium freudenreichii.
abstract: An efficient fermentation-strengthening approach was developed to improve the anaerobic production of propionic acid and vitamin B12 by co-fermentation with Propionibacterium freudenreichii. Vitamin B12 production from glucose resulted in relatively high productivity (0.35 mg/L h) but a low propionic acid yield (0.55 g/g). By contrast, glycerol gave a high propionic acid yield (0.63 g/g) but low productivity (0.16 g/L h). Co-fermentation of glycerol and glucose with a gradual addition strategy gave high yields (propionic acid: 0.71 g/g; vitamin B12: 0.72 mg/g) and productivities (propionic acid: 0.36 g/L h; vitamin B12: 0.36 mg/L h). Finally, the integrated feedstock and fermentation system strengthening strategy was demonstrated as an efficient method for the economic production of bio-based propionic acid and vitamin B12.
authors.affiliation: College of Chemical and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, 050018, China, wangpeng506@sina.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Youjing
authors.initials: Y
authors.lastname: Jiao
authors.forename: Shouxin
authors.initials: S
authors.lastname: Liu
mesh_terms: D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D011422:Propionates / Q000378:metabolism*; D011424:Propionibacterium / Q000378:metabolism*; D014805:Vitamin B 12 / Q000096:biosynthesis*
chemical_list: D011422:Propionates; D005947:Glucose; C029658:propionic acid; D014805:Vitamin B 12; D005990:Glycerol