title: Influence of diabetes mellitus on tissue response to MTA and its ability to stimulate mineralization.
abstract: OBJECTIVE
  To evaluate the influence of diabetes mellitus on the tissue response to mineral trioxide aggregate (MTA) and its ability to stimulate mineralization.
  
  
  METHODS
  Twenty-four male Wistar rats were divided into a non-diabetic control group and another with Alloxan-induced diabetes. Two polyethylene tubes, one kept empty as a control and the other containing Angelus MTA(®) , were implanted into the dorsal connective tissue of all rats for 30 days. Animals in each group received injections of calcein, alizarin, and oxytetracycline on day 7, 14, and 21, respectively. Animals were killed after 30 days; specimens were prepared by staining with hematoxylin and eosin (HE) and von Kossa technique as well as for examination of unstained sections with polarized light and fluorescence microscopy.
  
  
  RESULTS
  The inflammatory reaction to the implanted tubes was equally mild in both groups. Structures staining with von Kossa were seen in response to Angelus MTA(®) , as were birefringent structures visualized on polarized light analysis; these had no relation to diabetic condition (P < 0.05). Fluorescence intensity was not changed in diabetic rats either (P < 0.05).
  
  
  CONCLUSIONS
  Diabetes mellitus did not influence the tissue response to Angelus MTA(®) or the mineralization stimulated by it.
authors.affiliation: Department of Endodontics, Araçatuba School of Dentistry, University Estadual Paulista, Araçatuba, Brazil.
authors.forename: João Eduardo
authors.initials: JE
authors.lastname: Gomes-Filho
authors.forename: Índia Olinta
authors.initials: ÍO
authors.lastname: de Azevedo Queiroz
authors.forename: Simone
authors.initials: S
authors.lastname: Watanabe
authors.forename: Ludmilla Mota
authors.initials: LM
authors.lastname: da Silva Santos
authors.forename: Carolina Simonetti
authors.initials: CS
authors.lastname: Lodi
authors.forename: Robertta
authors.initials: R
authors.lastname: Okamoto
authors.forename: Edilson
authors.initials: E
authors.lastname: Ervolino
authors.forename: Elói
authors.initials: E
authors.lastname: Dezan
authors.forename: Luciano Tavares Angelo
authors.initials: LT
authors.lastname: Cintra
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology*; D000818:Animals; D001672:Biocompatible Materials; D017610:Calcium Compounds / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental; D004338:Drug Combinations; D007249:Inflammation / Q000139:chemically induced; D008297:Male; D008422:Materials Testing; D008856:Microscopy, Fluorescence; D009336:Necrosis; D010087:Oxides / Q000494:pharmacology*; D020959:Polyethylene; D051381:Rats; D017208:Rats, Wistar; D017640:Silicates / Q000494:pharmacology*; D040521:Subcutaneous Tissue / Q000187:drug effects*
chemical_list: D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D017640:Silicates; C086631:mineral trioxide aggregate; D020959:Polyethylene
keywords: MTA; biocompatibility; diabetes mellitus; endodontics