title: Tert-butylhydroquinone attenuates scrotal heat-induced damage by regulating Nrf2-antioxidant system in the mouse testis.
abstract: Tert-butylhydroquinone (tBHQ), a widely used nuclear factor erythroid 2-related factor 2 (Nrf2) activator, was always employed to investigate the potential protective role of Nrf2 activation. In this study, to elucidate the effect of tBHQ on scrotal heat-induced damage and Nrf2-antioxidant system in mouse testes, eight-week-old mice were administrated with or without dietary tBHQ (1%w/w) for 1week and afterward subjected to a single scrotal heat treatment (42°C for 25min). Trunk blood and testes were collected 3h or 1, 2, or 7days later. Mice displayed less germ cell loss in testes, higher relative testis weight and lower testosterone concentration on day 2 in tBHQ treatment group. Before heat treatment, there were significant increases in malondialdehyde (MDA) concentration in tBHQ treatment group. After heat treatment, mice in tBHQ treatment group showed lower MDA concentration than those in non-tBHQ treatment group. In addition, both tBHQ pretreatment and scrotal heat treatment induced markedly increased Nrf2 protein expression in cytoplasm and nuclei of interstitial cells, accompanying with elevated mRNA expression of Nrf2 and Nrf2-regulated genes in mice testes. Our data indicated that pretreatment to tBHQ induced a mild oxidative stress, and further enhanced the cellular antioxidative ability to protect testicular cells against scrotal heat-induced damage via a mechanism that might involve the Nrf2-antioxidant system in mice testes.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yansen
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Cao
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Shaoxia
authors.initials: S
authors.lastname: Pu
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: chunmeili@njau.edu.cn.
authors.forename: ChunMei
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D006358:Hot Temperature; D006873:Hydroquinones / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D008813:Mice, Inbred ICR; D008954:Models, Biological; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012611:Scrotum / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D012671:Seminiferous Tubules / Q000187:drug effects / Q000378:metabolism; D013737:Testis / Q000187:drug effects / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: D000975:Antioxidants; D006873:Hydroquinones; D051267:NF-E2-Related Factor 2; D020011:Protective Agents; D012333:RNA, Messenger; C018855:2-tert-butylhydroquinone
keywords: Nrf2; Scrotal heat; Tert-butylhydroquinone; Testis