title: Correlation between fibronectin binding protein A expression level at the surface of recombinant lactococcus lactis and plasmid transfer in vitro and in vivo.
abstract: BACKGROUND
  Fibronectin Binding Protein A (FnBPA) is an invasin from Staphylococcus aureus that allows this pathogen to internalize into eukaryote cells. It was previously demonstrated that recombinant Lactococcus lactis expressing FnBPA were invasive and able to transfer a plasmid to eukaryotic cells in vitro and in vivo. In this study, the invasivity of recombinant strains of Lactococcus lactis that express FnBPA under the control of its constitutive promoter or driven by the strong nisin inducible expression system (NICE) were studied.
  
  
  RESULTS
  It was demonstrated that the nisA promoter allows an increase of FnBPA expression on the surface of Lactococcus lactis surface, as shown by flow cytometry, which subsequently enhanced internalization and plasmid transfer properties in vitro in Caco2 cells and Bone Marrow Dendritic Cells. In vivo, the use of nisA promoter increase the plasmid transfer in cells of both the small and large intestine of mice.
  
  
  CONCLUSIONS
  FnBPA expression at the surface of recombinant L. lactis is positively correlated to internalization and DNA transfer properties. The recombinant strains of L. lactis that expresses FnBPA under the control of the nisin inducible expression system could thus be considered as an improved tool in the field of DNA transfer.
authors.forename: Juliana F
authors.initials: JF
authors.lastname: Almeida
authors.forename: Denis
authors.initials: D
authors.lastname: Mariat
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
authors.forename: Anderson
authors.initials: A
authors.lastname: Miyoshi
authors.forename: Alejandra
authors.initials: A
authors.lastname: de Moreno de LeBlanc
authors.forename: Silvina
authors.initials: S
authors.lastname: Del Carmen
authors.forename: Rebeca
authors.initials: R
authors.lastname: Martin
authors.forename: Philippe
authors.initials: P
authors.lastname: Langella
authors.forename: Jean-Guy
authors.initials: JG
authors.lastname: LeBlanc
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Chatel
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics*; D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D056890:Eukaryota / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D013294:Lactococcus lactis / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D009561:Nisin / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D013203:Staphylococcal Infections / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics*
chemical_list: D018829:Adhesins, Bacterial; C078635:fibronectin-binding proteins, bacterial; D009561:Nisin; C102526:nisin A