title: microRNA-548l is involved in the migration and invasion of non-small cell lung cancer by targeting the AKT1 signaling pathway.
abstract: OBJECTIVE
  microRNAs (miRNAs) have been documented playing a critical role in cancer development and progression. In this study, we investigated the role of miR-548l in non-small cell lung cancer (NSCLC) migration and invasion.
  
  
  METHODS
  microRNAs microarray analysis was used to detect the differentially expressed miRNAs between various metastatic levels of NSCLC cells and further confirmed by real-time PCR analysis. To facilitate the delineation of the role of selected miR-548l in NSCLC pathology, we detected its expression in 22 NSCLC tissues. Proliferation, apoptosis, invasion and metastasis effects of the miRNA were evaluated using MTT, flow cytometry, wound healing and invasion assay following transfection with mimics and inhibitors. Luciferase assay and Western blot analysis were performed to assess miR-548l binding to AKT1 gene. AKT1 expression in the clinical tissues was evaluated using immunohistochemical staining.
  
  
  RESULTS
  The results showed a negative relationship between miR-548l expression and lymph node metastasis of NSCLC. Functional assays showed that over-expression of miR-548l suppressed NSCLC cell migration and invasion. Luciferase assays confirmed that miR-548l could directly bind to the 3' untranslated region of AKT1. Further data showed that the over-expression of AKT1 could rescue the effects of miR-548l in NSCLC cells, and the miR-548l expression was inversely correlated with AKT1 expression in NSCLC tissues. These results indicated that AKT1 was involved in miR-548l-induced suppression of NSCLC cell migration and invasion.
  
  
  CONCLUSIONS
  These results suggested that miR-548l may play a causal role through AKT1 in NSCLC invasion and metastasis.
authors.affiliation: Department of Respiratory Medicine, Second Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Caihong
authors.initials: C
authors.lastname: Liu
authors.forename: Huan
authors.initials: H
authors.lastname: Yang
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Xu
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Meiyu
authors.initials: M
authors.lastname: Zhou
authors.forename: Kui
authors.initials: K
authors.lastname: Xiao
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Shi
authors.forename: Lanyan
authors.initials: L
authors.lastname: Zhu
authors.forename: Lifang
authors.initials: L
authors.lastname: Yang
authors.forename: Rui
authors.initials: R
authors.lastname: Zhou
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000556:secondary; D000368:Aged; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000556:secondary; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D011379:Prognosis; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014407:Tumor Cells, Cultured; D014945:Wound Healing
chemical_list: C561887:MIRN548 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C494918:AKT1 protein, human; D051057:Proto-Oncogene Proteins c-akt