title: What is the primary function of the early teleost gill? Evidence for Na+/NH+4 exchange in developing rainbow trout (Oncorhynchus mykiss).
abstract: Post-hatch fishes lack a functional gill and use cutaneous surfaces for exchange with the surrounding environment. The ionoregulatory hypothesis posits that ionoregulation is the first physiological process to be limited by cutaneous exchange, necessitating its shift to the gills. We hypothesized that the ontogeny of branchial ammonia excretion (J amm) is coupled to Na(+) uptake (J Na in) in accordance with the current model for Na+/NH4+ in exchange in freshwater. Using divided chambers, branchial and cutaneous J amm, J Na in and oxygen consumption (MO2) by larval rainbow trout were assessed. Following hatch, the skin accounted for 97% and 86% of total J amm and J Na in, respectively. J amm and J Na in shifted to the gills simultaneously at 15 days post-hatch (dph) and were highly correlated (R(2) = 0.951) at the gills, but not the skin, over development. Contrastingly, MO2 shifted significantly later at 27 dph, in agreement with the ionoregulatory hypothesis. Moreover, the mRNA expression and/or enzymatic activity of Rhesus proteins, Na(+)/H(+)-exchanger, H(+)-ATPase, Na(+)/K(+)-ATPase and carbonic anhydrase, all key components of the Na+/NH4+-exchange system, increased in the gills over larval development. We propose that the ontogeny of branchial J Na in occurs as Na+/NH4+ exchange and provide evidence for a novel element to the ionoregulatory hypothesis, the excretion of potentially lethal metabolic ammonia.
authors.affiliation: Department of Biology, McMaster University, Hamilton, Ontario, Canada L8S 4K1 zimmea@mcmaster.ca.
authors.forename: Alex M
authors.initials: AM
authors.lastname: Zimmer
authors.affiliation: Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Wright
authors.affiliation: Department of Biology, McMaster University, Hamilton, Ontario, Canada L8S 4K1.
authors.forename: Chris M
authors.initials: CM
authors.lastname: Wood
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D000818:Animals; D005880:Gills / Q000254:growth & development / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000254:growth & development / Q000378:metabolism*; D010101:Oxygen Consumption; D012964:Sodium / Q000378:metabolism*
chemical_list: D000641:Ammonia; D012964:Sodium
keywords: ammonia excretion; gill development; ionoregulatory hypothesis; oxygen consumption; rainbow trout (Oncorhynchus mykiss) larvae; sodium uptake