title: Multichannel series piezoelectric quartz crystal cell sensor for real time and quantitative monitoring of the living cell and assessment of cytotoxicity.
abstract: A new multichannel series piezoelectric quartz crystal (MSPQC) cell sensor for real time monitoring of living cells in vitro was reported in this paper. The constructed sensor was used successfully to monitor adhesion, spreading, proliferation, and apoptosis of MG63 osteosarcoma cells and investigate the effects of different concentrations of cobalt chloride on MG63 cells. Quantitative real time and dynamic cell analyses data were conducted using the MSPQC cell sensor. Compared with methods such as fluorescence staining and morphology observation by microscopy, the MSPQC cell sensor is noninvasive, label free, simple, cheap, and capable of online monitoring. It can automatically record the growth status of cells and quantitatively evaluate cell proliferation and the apoptotic response to drugs. It will be a valuable detection and analysis tool for the acquisition of cellular level information and is anticipated to have application in the field of cell biology research or cytotoxicity testing in the future.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha, Hunan 410082, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Tong
authors.forename: Yan
authors.initials: Y
authors.lastname: Lian
authors.forename: Huang
authors.initials: H
authors.lastname: Zhou
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Shi
authors.forename: Fengjiao
authors.initials: F
authors.lastname: He
mesh_terms: D000970:Antineoplastic Agents / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D015374:Biosensing Techniques / Q000295:instrumentation*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003584:Cytological Techniques / Q000295:instrumentation*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D011791:Quartz / Q000737:chemistry*; D018675:Toxicity Tests / Q000295:instrumentation*
chemical_list: D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D011791:Quartz