title: A method for evaluating the level of soluble β-amyloid(1-40/1-42) in Alzheimer's disease based on the binding of gelsolin to β-amyloid peptides.
abstract: In the present work, a new electrochemical strategy for the sensitive and specific detection of soluble β-amyloid Aβ(1-40/1-42) peptides in a rat model of Alzheimer's disease (AD) is described. In contrast to previous antibody-based methods, β-amyloid(1-40/1-42) was quantified based on its binding to gelsolin, a secretory protein present in the cerebrospinal fluid (CSF) and plasma. The level of soluble β-amyloid peptides in the CSF and various brain regions were found with this method to be lower in rats with AD than in normal rats.
authors.affiliation: Department of Pharmaceutical Analysis, Xuzhou Medical College, Xuzhou 221004 (China); Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou 221004 (China).
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Yu
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Chenglin
authors.initials: C
authors.lastname: Li
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Sun
authors.forename: Daoquan
authors.initials: D
authors.lastname: Tang
authors.forename: Guoyue
authors.initials: G
authors.lastname: Shi
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D055664:Electrochemical Techniques / Q000379:methods*; D018260:Gelsolin / Q000378:metabolism*; D051381:Rats; D012995:Solubility
chemical_list: D016229:Amyloid beta-Peptides; D018260:Gelsolin
keywords: Alzheimer’s disease; aggregation; biosensors; electrochemistry; β-amyloid peptides