title: MicroRNA-29b regulates TGF-β1-mediated epithelial-mesenchymal transition of retinal pigment epithelial cells by targeting AKT2.
abstract: The role of microRNA (miRNA) in proliferative vitreoretinopathy (PVR) progression has not been studied extensively, especially in retinal pigment epithelial-mesenchymal transition (EMT) which is the main reason for formation of PVR. In this study, we first investigated the miRNA expression profile in transforming growth factor beta 1 (TGF-β1) mediated EMT of ARPE-19 cells. Among the five changed miRNAs, miR-29b showed the most significant downregulation. Enhanced expression of miR-29b could reverse TGF-β1 induced EMT through targeting Akt2. Akt2 downregulation could inhibit TGF-β1-induced EMT. Furthermore, inhibition of miR-29b in ARPE-19 cells directly triggered EMT process, which characterized by the phenotypic transition and the upregulation of α-smooth muscle actin (α-SMA) and downregulation of E-cadherin and zona occludin-1 (ZO-1) with increased cell migration. Akt2-shRNA also inhibited miR-29 inhibitor-induced EMT process. These data indicate that miR-29b plays an important role in TGF-β1-mediated EMT in ARPE-19 cells by targeting Akt2.
authors.affiliation: Department of Ophthalmology, Shanghai Tenth People׳s Hospital affiliated with Tongji University, School of Medicine, No. 301 Middle Yanchang Road, Shanghai 200072, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, Shanghai Tenth People׳s Hospital affiliated with Tongji University, School of Medicine, No. 301 Middle Yanchang Road, Shanghai 200072, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, Shanghai Tenth People׳s Hospital affiliated with Tongji University, School of Medicine, No. 301 Middle Yanchang Road, Shanghai 200072, PR China.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Ophthalmology, Shanghai Tenth People׳s Hospital affiliated with Tongji University, School of Medicine, No. 301 Middle Yanchang Road, Shanghai 200072, PR China.
authors.forename: Ding
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Ophthalmology, Shanghai Tenth People׳s Hospital affiliated with Tongji University, School of Medicine, No. 301 Middle Yanchang Road, Shanghai 200072, PR China. Electronic address: milwang_122@msn.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
mesh_terms: D001483:Base Sequence; D015536:Down-Regulation / Q000187:drug effects; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects* / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D055213:Retinal Pigment Epithelium / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*
chemical_list: C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D053773:Transforming Growth Factor beta1; C494921:AKT2 protein, human; D051057:Proto-Oncogene Proteins c-akt
keywords: Akt2; EMT; MicroRNA-29b; Retinal pigment epithelial cells