title: Evaluation of the Xpert Flu rapid PCR assay in high-risk emergency department patients.
abstract: We prospectively evaluated the performance of Cepheid's GeneXpert Xpert Flu assay in a target population of 281 adults presenting to the emergency department with an acute respiratory illness who met Centers for Disease Control and Prevention (CDC) criteria for recommended antiviral treatment. Compared with the Prodesse ProFlu+ assay, Xpert Flu had an overall sensitivity of 95.3% and specificity of 99.2%.
authors.affiliation: Department of Emergency Medicine, Johns Hopkins University, Baltimore, Maryland, USA adugas1@jhmi.edu.
authors.forename: Andrea F
authors.initials: AF
authors.lastname: Dugas
authors.affiliation: Department of Pathology, Johns Hopkins University, Baltimore, Maryland, USA.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Valsamakis
authors.affiliation: Department of Emergency Medicine, Johns Hopkins University, Baltimore, Maryland, USA Department of Medicine, Division of Infectious Disease, Johns Hopkins University, Baltimore, Maryland, USA.
authors.forename: Charlotte A
authors.initials: CA
authors.lastname: Gaydos
authors.affiliation: Department of Pathology, Johns Hopkins University, Baltimore, Maryland, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Forman
authors.affiliation: Department of Medicine, Division of Infectious Disease, Johns Hopkins University, Baltimore, Maryland, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Hardick
authors.affiliation: Department of Emergency Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
authors.forename: Pranav
authors.initials: P
authors.lastname: Kidambi
authors.affiliation: Department of Emergency Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
authors.forename: Sharmeen
authors.initials: S
authors.lastname: Amin
authors.affiliation: Department of Emergency Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
authors.forename: Alisha
authors.initials: A
authors.lastname: Gupta
authors.affiliation: Department of Emergency Medicine, Johns Hopkins University, Baltimore, Maryland, USA Department of Medicine, Division of Infectious Disease, Johns Hopkins University, Baltimore, Maryland, USA.
authors.forename: Richard E
authors.initials: RE
authors.lastname: Rothman
mesh_terms: D000328:Adult; D004632:Emergency Medical Services / Q000379:methods*; D004636:Emergency Service, Hospital; D005260:Female; D006801:Humans; D007251:Influenza, Human / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009975:Orthomyxoviridae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011446:Prospective Studies; D012680:Sensitivity and Specificity