title: MicroRNA profiling reveals unique miRNA signatures in IGF-1 treated embryonic striatal stem cell fate decisions in striatal neurogenesis in vitro.
abstract: The striatum is considered to be the central processing unit of the basal ganglia in locomotor activity and cognitive function of the brain. IGF-1 could act as a control switch for the long-term proliferation and survival of EGF+bFGF-responsive cultured embryonic striatal stem cell (ESSC), while LIF imposes a negative impact on cell proliferation. The IGF-1-treated ESSCs also showed elevated hTERT expression with demonstration of self-renewal and trilineage commitment (astrocytes, oligodendrocytes, and neurons). In order to decipher the underlying regulatory microRNA (miRNA)s in IGF-1/LIF-treated ESSC-derived neurogenesis, we performed in-depth miRNA profiling at 12 days in vitro and analyzed the candidates using the Partek Genome Suite software. The annotated miRNA fingerprints delineated the differential expressions of miR-143, miR-433, and miR-503 specific to IGF-1 treatment. Similarly, the LIF-treated ESSCs demonstrated specific expression of miR-326, miR-181, and miR-22, as they were nonsignificant in IGF-treated ESSCs. To elucidate the possible downstream pathways, we performed in silico mapping of the said miRNAs into ingenuity pathway analysis. Our findings revealed the important mRNA targets of the miRNAs and suggested specific interactomes. The above studies introduced a new genre of miRNAs for ESSC-based neuroregenerative therapeutic applications.
authors.affiliation: Department of Neuroscience, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia ; Department of Pediatrics-Neurology, Baylor College of Medicine, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, 1250 Moursund Street, Room 1250, Houston, TX 77030, USA.
authors.forename: Soumya
authors.initials: S
authors.lastname: Pati
authors.affiliation: Department of Neuroscience, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia ; Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
authors.forename: Nor Entan
authors.initials: NE
authors.lastname: Supeno
authors.affiliation: Department of Neuroscience, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia ; Laboratory of Neuropsychopharmacology, FFCLRP, University of Sao Paulo (USP), Avenida Bandeirantes 3900, Monte Alegre, Ribeirao Preto, SP 14040-900, Brazil.
authors.forename: Sangu
authors.initials: S
authors.lastname: Muthuraju
authors.affiliation: Department of Neuroscience, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia ; Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
authors.forename: Raisah
authors.initials: R
authors.lastname: Abdul Hadi
authors.affiliation: Department of Neuroscience, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
authors.forename: Abdul Rahman Izaini
authors.initials: AR
authors.lastname: Ghani
authors.affiliation: Department of Medical Microbiology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
authors.forename: Fauziah Mohamad
authors.initials: FM
authors.lastname: Idris
authors.affiliation: Departments of Pediatrics-Neurology and Neuroscience, Program in Developmental Biology, Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, 1250 Moursund Street, Room 1250, Houston, TX 77030, USA.
authors.forename: Mirjana
authors.initials: M
authors.lastname: Maletic-Savatic
authors.affiliation: Department of Neuroscience, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia ; Center for Neuroscience Services and Research, Universiti Sains Malaysia, Sultanah Zainab 2 Road, 16150 Kubang Kerian, Kelantan, Malaysia ; Neuroscience Department, Universiti Sains Malaysia Hospital, USM Hospital Road, 16150 Kubang Kerian, Kelantan, Malaysia.
authors.forename: Jafri Malin
authors.initials: JM
authors.lastname: Abdullah
authors.affiliation: Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
authors.forename: Hasnan
authors.initials: H
authors.lastname: Jaafar
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003198:Computer Simulation; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage*; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D055495:Neurogenesis / Q000187:drug effects; D019098:Telomerase / Q000096:biosynthesis; D014793:Visual Cortex / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*
chemical_list: D035683:MicroRNAs; D007334:Insulin-Like Growth Factor I; C509186:TERT protein, human; D019098:Telomerase