title: Influence of smoothened siRNA on human esophageal cancer cell line EC9706 proliferation and apoptosis.
abstract: Apply siRNA technology to take Smoothened (Smo) genetic locus, the possibility of Hhsignaling pathway, and also inhibits esophageal squamous carcinoma cell proliferation and induction of apoptosis. Utilizing SmosiRNA transfected esophageal squamous carcinoma EC9706 cells, adopting RT-PCR and Western blot technology to detect Smo, Gli1 mRNA and protein expression in each group of cells, employing MTT and FCM to detect the influence of SmosiRNA on cell proliferation and apoptosis. Compared with each control group, after transfected SmosiRNA transfected cell for 24h, 48 h and 72 h, SmosiRNA and Gli1 mRNA expression was evenly reduced obviously, Smo and Gli1 protein expression level was also reduced clearly. SmosiRNA could inhabit cell proliferation, and even promote cell apoptosis. Smo gene played a role in regulating and controlling esophageal squamous carcinoma cell proliferation and apoptosis and Smo was likely to be an effective target spot for tumor gene therapy.
authors.affiliation: Department of Radiation, Affiliated Tumor Hospital of Zhengzhou University, Zhengzhou, P.R. China.
authors.forename: Wu
authors.initials: W
authors.lastname: Hui
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, P. R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Kuisheng
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, P. R. China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Hongxin
authors.affiliation: Department of Pathology, Henan Provincial People's Hospital, Zhengzhou, P. R. China.
authors.forename: Ding
authors.initials: D
authors.lastname: Yanzhi
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, P. R. China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zhihua
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, P. R. China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Hu
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000473:pathology* / Q000628:therapy; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004938:Esophageal Neoplasms / Q000473:pathology* / Q000628:therapy; D000077277:Esophageal Squamous Cell Carcinoma; D015316:Genetic Therapy; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D034741:RNA, Small Interfering / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D000072086:Smoothened Receptor; D014157:Transcription Factors / Q000235:genetics; D000071676:Zinc Finger Protein GLI1
chemical_list: C400551:GLI1 protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D043562:Receptors, G-Protein-Coupled; C491023:SMO protein, human; D000072086:Smoothened Receptor; D014157:Transcription Factors; D000071676:Zinc Finger Protein GLI1