title: γ-Interferon-inducible lysosomal thiol reductase (GILT) maintains phagosomal proteolysis in alternatively activated macrophages.
abstract: Although it is known that lysosomal cysteine cathepsins require a reducing environment for optimal activity, it is not firmly established how these enzymes are maintained in their reduced-active state in the acidic and occasionally oxidative environment within phagosomes and lysosomes. γ-Interferon-inducible lysosomal thiol reductase (GILT) has been the only enzyme described in the endosomes, lysosomes, and phagosomes with the potential to catalyze the reduction of cysteine cathepsins. Our goal in the current study was to assess the effect of GILT on major phagosomal functions with an emphasis on proteolytic efficiency in murine bone marrow-derived macrophages. Assessment of phagosomal disulfide reduction upon internalization of IgG-opsonized experimental particles confirmed a major role for GILT in phagosomal disulfide reduction in both resting and interferon-γ-activated macrophages. Furthermore we observed a decrease in early phagosomal proteolytic efficiency in GILT-deficient macrophages, specifically in the absence of an NADPH oxidase-mediated respiratory burst. This deficiency was more prominent in IL-4-activated macrophages that inherently possess lower levels of NADPH oxidase activity. Finally, we provide evidence that GILT is required for optimal activity of the lysosomal cysteine protease, cathepsin S. In summary, our results suggest a role for GILT in maintaining cysteine cathepsin proteolytic efficiency in phagosomes, particularly in the absence of high NADPH oxidase activity, which is characteristic of alternatively activated macrophages.
authors.affiliation: Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine and University of Calgary, Calgary, Alberta T2N 4N1, Canada.
authors.forename: Dale R
authors.initials: DR
authors.lastname: Balce
authors.affiliation: Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine and University of Calgary, Calgary, Alberta T2N 4N1, Canada.
authors.forename: Euan R O
authors.initials: ERO
authors.lastname: Allan
authors.affiliation: Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine and University of Calgary, Calgary, Alberta T2N 4N1, Canada; Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, Alberta T2N 4N1, Canada.
authors.forename: Neil
authors.initials: N
authors.lastname: McKenna
authors.affiliation: Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine and University of Calgary, Calgary, Alberta T2N 4N1, Canada; Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, Alberta T2N 4N1, Canada. Electronic address: rmyates@ucalgary.ca.
authors.forename: Robin M
authors.initials: RM
authors.lastname: Yates
mesh_terms: D000818:Animals; D000941:Antigens / Q000378:metabolism; D002403:Cathepsins / Q000378:metabolism; D057056:Cysteine Proteases / Q000378:metabolism; D004220:Disulfides / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019255:NADPH Oxidases / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D050862:Oxidoreductases Acting on Sulfur Group Donors; D010100:Oxygen / Q000378:metabolism; D010588:Phagosomes / Q000378:metabolism*; D059748:Proteolysis; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000941:Antigens; D004220:Disulfides; D017382:Reactive Oxygen Species; D007371:Interferon-gamma; D010088:Oxidoreductases; D019255:NADPH Oxidases; C483811:Ifi30 protein, mouse; D050862:Oxidoreductases Acting on Sulfur Group Donors; D002403:Cathepsins; D057056:Cysteine Proteases; D010100:Oxygen
keywords: Cysteine Protease; Lysosome; Macrophage; Oxidation-Reduction (Redox); Phagosome; Proteolysis; Reductase