title: [Determination of gossypol in edible vegetable oil with high performance liquid chromatography-tandem mass spectrometry].
abstract: A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the determination of gossypol in edible vegetable oil. The sample was extracted with ethyl alcohol by vortex-excited oscillation. The extract was cleaned up by 0.22 microm filter membrane and centrifuged for 5 min at 4 000 r/min after standing in a fridge at 4 degrees C for 30 min. The compound was separated on a C18 column (100 mm x 2.1 mm, 3.5 microm) with acetonitrile and 1% (v/v) formic acid aqueous solution as mobile phase. The detection of gossypol was carried out by LC-MS/MS with positive electrospray ionization under multiple reaction monitoring (MRM) mode using external standard method. The limits of quantification (S/N > 10) of gossypol in edible vegetable oil was 1 mg/kg. The recoveries were from 87.4% to 100% at the spiked levels of 1, 2, 200 mg/kg of gossypol in edible vegetable oil with the relative standard deviations (RSDs) between 3.9% and 12.2%. The method, with high sensitivity, good precision and high recovery, was suitable for the confirmation and quantification of gossypol residue in edible vegetable oil.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Zhang
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Huang
authors.forename: Wen
authors.initials: W
authors.lastname: Xie
authors.forename: Li
authors.initials: L
authors.lastname: Shen
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006072:Gossypol / Q000032:analysis*; D010938:Plant Oils / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D014675:Vegetables
chemical_list: D010938:Plant Oils; D006072:Gossypol