title: [Simultaneous determination of seven constituents in Euodiae Fructus and two related species by HPLC].
abstract: This study is to develop a HPLC method for quality evaluation of Euodiae Fructus and related species by simultaneous determination limonin, indole alkaloids (14-fomyldihydroxyrutaecarpine, evodiamine, rutaecarpine), and quinolone alkaloids [1-methyl-2-undecyl-4 (1H)-quinolone, evocarpine, dihydroevocarpine] in the fruits of five Evodia species. Samples were analyzed on a YMC C18 column (4.6 mm x 250 mm, 5 microm) eluted with mobile phases of acetonitrile (A), tetrahydrofuran (B), and a buffer solution of 5 mmol x L(-1) ammonium acetate (pH 3.8) (C) in a linear gradient mode. The column temperature was 30 degrees C and the flow rate was 1.0 mL x min(-1). The PDA detector wavelengths were set at 220 and 250 nm. The seven compounds were well separated and showed good linearity (r = 0.999 9) within the concentration ranges tested. The mean recoveries were between 96.7%-102.4% (RSD 1.4%-3.1%). Through the validation, the method was proved to be accurate and repeatable. All the seven constituents were detected in the fruits of five species, but the contents of them varied widely in different samples. The total contents of seven constituents in 16 batches of Euodiae Fructus were 9.46-69.9 mg x g(-1), and the mean content was 28.2 mg x g(-1). The total content of seven constituents in E. compacta and E. fargesii was 25.8, 7.69 mg x g(-1), respectively.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Yin
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Yan
authors.forename: Qi-Wei
authors.initials: QW
authors.lastname: Zhang
authors.forename: Yong-Xin
authors.initials: YX
authors.lastname: Zhang
authors.forename: Li-Mei
authors.initials: LM
authors.lastname: Lin
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Zhang
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D032086:Evodia / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal