title: A feasibility study of full-field optical coherence tomography for rapid evaluation of EUS-guided microbiopsy specimens.
abstract: BACKGROUND
  Rapid on-site evaluation of cytologic specimens is a way of determining the adequacy of fine-needle aspiration (FNA). However, alternatives may be useful when the presence of a cytotechnologist and/or pathologist is not possible.
  
  
  OBJECTIVE
  To evaluate the feasibility of using full-field optical coherence tomography (FFOCT) for FNA specimen quality assessment.
  
  
  METHODS
  FFOCT images were acquired on gastric, pancreatic, pelvic, and lymph-node formalin-fixed FNA specimens and were compared with histology of the same samples.
  
  
  METHODS
  Pathology suite in a hospital.
  
  
  METHODS
  Fourteen patients undergoing gastric, pancreatic, pelvic, or lymph-node EUS-guided FNA biopsy.
  
  
  METHODS
  FFOCT imaging on formalin-fixed samples before histologic procedures.
  
  
  METHODS
  FFOCT imaging feasibility and visibility of normal and abnormal features on images.
  
  
  RESULTS
  FFOCT imaging was possible. Blood, mucus, muscle, collagen, and digestive mucosa could be identified as well as abnormal architectural features including infiltrative pancreatic ductal carcinoma and a neuroendocrine neoplasm. Lesions at the individual cell level could not be detected.
  
  
  CONCLUSIONS
  The study was performed on a limited number of cases.
  
  
  CONCLUSIONS
  FFOCT offers rapid, noninvasive, nondestructive imaging of FNA biopsy specimens. In the future, it could be performed in the endoscopy suite to improve detection of satisfactory specimens and obviate the need for rapid on-site evaluation.
authors.affiliation: Institut de la Vision, Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Paris, France.
authors.forename: Kate
authors.initials: K
authors.lastname: Grieve
authors.affiliation: Clinique du Trocadéro, Paris, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Palazzo
authors.affiliation: LLTech SAS, Paris, France.
authors.forename: Eugenie
authors.initials: E
authors.lastname: Dalimier
authors.affiliation: Department of Biology and Pathology, Translational Research Laboratory, Gustave Roussy, Villejuif, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Vielh
authors.affiliation: Department of Biology and Pathology, Translational Research Laboratory, Gustave Roussy, Villejuif, France.
authors.forename: Monique
authors.initials: M
authors.lastname: Fabre
mesh_terms: D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000175:diagnosis; D061765:Endoscopic Ultrasound-Guided Fine Needle Aspiration; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D008297:Male; D008479:Mediastinal Neoplasms / Q000175:diagnosis*; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000175:diagnosis*; D010386:Pelvic Neoplasms / Q000175:diagnosis*; D015203:Reproducibility of Results; D013274:Stomach Neoplasms / Q000175:diagnosis*; D041623:Tomography, Optical Coherence