title: Genome packaging in EL and Lin68, two giant phiKZ-like bacteriophages of P. aeruginosa.
abstract: A unique feature of the Pseudomonas aeruginosa giant phage phiKZ is its way of genome packaging onto a spool-like protein structure, the inner body. Until recently, no similar structures have been detected in other phages. We have studied DNA packaging in P. aeruginosa phages EL and Lin68 using cryo-electron microscopy and revealed the presence of inner bodies. The shape and positioning of the inner body and the density of the DNA packaging in EL are different from those found in phiKZ and Lin68. This internal organization explains how the shorter EL genome is packed into a large EL capsid, which has the same external dimensions as the capsids of phiKZ and Lin68. The similarity in the structural organization in EL and other phiKZ-like phages indicates that EL is phylogenetically related to other phiKZ-like phages, and, despite the lack of detectable DNA homology, EL, phiKZ, and Lin68 descend from a common ancestor.
authors.affiliation: M.V. Lomonosov Moscow State University, Moscow, Russia; A.V. Shoubnikov Institute of Crystallography RAS, Moscow, Russia. Electronic address: sokolova@mail.bio.msu.ru.
authors.forename: O S
authors.initials: OS
authors.lastname: Sokolova
authors.affiliation: I.I. Mechnikov Research Institute of Vaccines and Sera, RAMS, Moscow, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Shaburova
authors.affiliation: A.V. Shoubnikov Institute of Crystallography RAS, Moscow, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Pechnikova
authors.affiliation: M.V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: A K
authors.initials: AK
authors.lastname: Shaytan
authors.affiliation: I.I. Mechnikov Research Institute of Vaccines and Sera, RAMS, Moscow, Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Krylov
authors.affiliation: A.V. Shoubnikov Institute of Crystallography RAS, Moscow, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Kiselev
authors.affiliation: I.I. Mechnikov Research Institute of Vaccines and Sera, RAMS, Moscow, Russia.
authors.forename: V N
authors.initials: VN
authors.lastname: Krylov
mesh_terms: D001435:Bacteriophages / Q000502:physiology* / Q000648:ultrastructure*; D020285:Cryoelectron Microscopy; D016679:Genome, Viral / Q000502:physiology*; D011550:Pseudomonas aeruginosa / Q000821:virology*
keywords: Bacteriophages; EL; Inner Body; Lin68; PhiKZ; Pseudomonas aeruginosa