title: Histopathological Study of the Lungs of Mice Receiving Human Secretory IgA and Challenged with Mycobacterium tuberculosis.
abstract: BACKGROUND
  Humoral and cellular immune responses are associated with protection against extracellular and intracellular pathogens, respectively. In the present study, we evaluated the effect of receiving human secretory immunoglobulin A (hsIgA) on the histopathology of the lungs of mice challenged with virulent Mycobacterium tuberculosis.
  
  
  METHODS
  The hsIgA was purified from human colostrum and administered to Balb/c mice by the intranasal route prior to infection with M. tuberculosis or in a pre-incubated formulation with mycobacteria, with the principal aim to study its effect on qualitative pulmonary histopathology.
  
  
  RESULTS
  The intranasal administration of hsIgA and the pre-incubation of mycobacteria with this preparation was associated with the presence of organised granulomas with signs of immune activation and histological features related to efficient disease control. This effect was highly evident during the late stage of infection (60 days), as demonstrated by numerous organised granulomas with numerous activated macrophages in the lungs of treated mice.
  
  
  CONCLUSIONS
  The administration of hsIgA to mice before intratracheal infection with M. tuberculosis or the pre-incubation of the bacteria with the antibody formulation induced the formation of well-organised granulomas and inflammatory lesions in lungs compared with non-treated animals which correlates with the protective effect already demonstrated by these antibody formulations.
authors.affiliation: Department of Molecular Biology, Finlay Institute, Center of Research-Production of Vaccines, Ave. 27 No. 19805, La Lisa, Ciudad de la Habana, Cuba. AP. 16017, CP11600.
authors.forename: Nadine
authors.initials: N
authors.lastname: Alvarez
authors.affiliation: Department of Molecular Biology, Finlay Institute, Center of Research-Production of Vaccines, Ave. 27 No. 19805, La Lisa, Ciudad de la Habana, Cuba. AP. 16017, CP11600.
authors.forename: Juan Francisco
authors.initials: JF
authors.lastname: Infante
authors.affiliation: Department of Molecular Biology, Finlay Institute, Center of Research-Production of Vaccines, Ave. 27 No. 19805, La Lisa, Ciudad de la Habana, Cuba. AP. 16017, CP11600.
authors.forename: Reinier
authors.initials: R
authors.lastname: Borrero
authors.affiliation: Experimental Pathology Section, Department of Pathology, National Institute of Medical Sciences and Nutrition "Salvador Zubirán", Mexico.
authors.forename: Dulce
authors.initials: D
authors.lastname: Mata
authors.affiliation: Experimental Pathology Section, Department of Pathology, National Institute of Medical Sciences and Nutrition "Salvador Zubirán", Mexico.
authors.forename: Jorge Barrios-
authors.initials: JB
authors.lastname: Payan
authors.affiliation: School of Health Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
authors.forename: Md Murad
authors.initials: MM
authors.lastname: Hossain
authors.affiliation: School of Health Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
authors.forename: Norazmi
authors.initials: N
authors.lastname: Mohd Nor
authors.affiliation: Department of Molecular Biology, Finlay Institute, Center of Research-Production of Vaccines, Ave. 27 No. 19805, La Lisa, Ciudad de la Habana, Cuba. AP. 16017, CP11600.
authors.forename: María Elena
authors.initials: ME
authors.lastname: Sarmiento
authors.affiliation: Experimental Pathology Section, Department of Pathology, National Institute of Medical Sciences and Nutrition "Salvador Zubirán", Mexico.
authors.forename: Rogelio
authors.initials: R
authors.lastname: Hernandez-Pando
authors.affiliation: Department of Molecular Biology, Finlay Institute, Center of Research-Production of Vaccines, Ave. 27 No. 19805, La Lisa, Ciudad de la Habana, Cuba. AP. 16017, CP11600.
authors.forename: Armando
authors.initials: A
authors.lastname: Acosta
keywords: Mycobacterium tuberculosis; colostrums; secretory immunoglobulin A