title: Circulating microRNAs in Pancreatic Juice as Candidate Biomarkers of Pancreatic Cancer.
abstract: Development of sensitive and specific biomarkers, preferably those circulating in body fluids is critical for early diagnosis of cancer. This study performed profiling of microRNAs (miRNAs) in exocrine pancreatic secretions (pancreatic juice) by microarray analysis utilizing pancreatic juice from 6 pancreatic ductal adenocarcinoma (PDAC) patients and two pooled samples from 6 non-pancreatic, non-healthy (NPNH) as controls. Differentially circulating miRNAs were subsequently validated in 88 pancreatic juice samples from 50 PDAC, 19 chronic pancreatitis (CP) patients and 19 NPNH controls. A marked difference in the profiles of four circulating miRNAs (miR-205, miR-210, miR-492, and miR-1427) was observed in pancreatic juice collected from patients with PDAC and those without pancreatic disease. Elevated levels of the four miRNAs together predicted PDAC with a specificity of 88% and sensitivity of 87%. Inclusion of serum CA19-9 level increased the sensitivity to 91% and the specificity to 100%. Enrichment of the four miRNAs in pancreatic juice was associated with decreased OS, as was the combination of miR-205 and miR-210. Higher contents of miR-205 and miR-210 were also associated with lymph node metastasis. Elevated levels of circulating miR-205, miR-210, miR-492, and miR-1247 in pancreatic juice are, therefore, promising candidate biomarkers of disease and poor prognosis in patients with PDAC.
authors.affiliation: 1. Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas;
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: 2. Division of Gastroenterology and Hepatology, Mayo Clinic, Jacksonville, Florida; The University of Texas Medical School and Health Science Center at Houston, Texas;
authors.forename: Massimo
authors.initials: M
authors.lastname: Raimondo
authors.affiliation: 3. Division of Gastroenterology, Hepatology, and Nutrition, The University of Texas Medical School and Health Science Center at Houston, Texas;
authors.forename: Sushovan
authors.initials: S
authors.lastname: Guha
authors.affiliation: 4. Epidemiology, Bioinformatics and Computational Biology, UT MD Anderson Cancer Center, Houston Texas;
authors.forename: Jinyun
authors.initials: J
authors.lastname: Chen
authors.affiliation: 5. Bioinformatics and Computational Biology, UT MD Anderson Cancer Center, Houston Texas;
authors.forename: Lixia
authors.initials: L
authors.lastname: Diao
authors.affiliation: 6. Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, The University of Rhode Island, Kingston, Rhode Island;
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Dong
authors.affiliation: 2. Division of Gastroenterology and Hepatology, Mayo Clinic, Jacksonville, Florida; The University of Texas Medical School and Health Science Center at Houston, Texas;
authors.forename: Michael B
authors.initials: MB
authors.lastname: Wallace
authors.affiliation: 1. Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas; ; 7. Program in Human and Molecular Genetics, The University of Texas Graduate school of Biomedical Sciences, Houston, Texas.
authors.forename: Ann M
authors.initials: AM
authors.lastname: Killary
authors.affiliation: 4. Epidemiology, Bioinformatics and Computational Biology, UT MD Anderson Cancer Center, Houston Texas; ; 7. Program in Human and Molecular Genetics, The University of Texas Graduate school of Biomedical Sciences, Houston, Texas.
authors.forename: Marsha L
authors.initials: ML
authors.lastname: Frazier
authors.affiliation: 2. Division of Gastroenterology and Hepatology, Mayo Clinic, Jacksonville, Florida; The University of Texas Medical School and Health Science Center at Houston, Texas;
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Woodward
authors.affiliation: 5. Bioinformatics and Computational Biology, UT MD Anderson Cancer Center, Houston Texas;
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: 1. Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas; ; 7. Program in Human and Molecular Genetics, The University of Texas Graduate school of Biomedical Sciences, Houston, Texas.
authors.forename: Subrata
authors.initials: S
authors.lastname: Sen
keywords: biomarker; circulating; miRNA; pancreatic cancer.; pancreatic juice