title: Mouse Genome Editing Using the CRISPR/Cas System.
abstract: The availability of techniques to create desired genetic mutations has enabled the laboratory mouse as an extensively used model organism in biomedical research including human genetics. A new addition to this existing technical repertoire is the CRISPR/Cas system. Specifically, this system allows editing of the mouse genome much more quickly than the previously used techniques, and, more importantly, multiple mutations can be created in a single experiment. Here we provide protocols for preparation of CRISPR/Cas reagents and microinjection into one-cell mouse embryos to create knockout or knock-in mouse models.
authors.affiliation: Mouse Genome Engineering Core Facility, Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, Nebraska; These authors contributed equally to this work.
authors.forename: Donald W
authors.initials: DW
authors.lastname: Harms
authors.forename: Rolen M
authors.initials: RM
authors.lastname: Quadros
authors.forename: Davide
authors.initials: D
authors.lastname: Seruggia
authors.forename: Masato
authors.initials: M
authors.lastname: Ohtsuka
authors.forename: Gou
authors.initials: G
authors.lastname: Takahashi
authors.forename: Lluis
authors.initials: L
authors.lastname: Montoliu
authors.forename: Channabasavaiah B
authors.initials: CB
authors.lastname: Gurumurthy
mesh_terms: D000818:Animals; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D016678:Genome; D051379:Mice
keywords: CRISPR/Cas9; gene editing; mutant mouse; pronuclear and cytoplasmic injection; sgRNA