title: Facile fabrication of poly(L-lactic acid)-grafted hydroxyapatite/poly(lactic-co-glycolic acid) scaffolds by Pickering high internal phase emulsion templates.
abstract: Porous scaffolds consisting of bioactive inorganic nanoparticles and biodegradable polymers have gained much interest in bone tissue engineering. We report here a facile approach to fabricating poly(l-lactic acid)-grafted hydroxyapatite (g-HAp)/poly(lactide-co-glycolide) (PLGA) nanocomposite (NC) porous scaffolds by solvent evaporation of Pickering high internal phase emulsion (HIPE) templates, where g-HAp nanoparticles act as particulate stabilizers. The resultant porous scaffolds exhibit an open and rough pore structure. The pore structure and mechanical properties of the scaffolds can be tuned readily by varying the g-HAp nanoparticle concentration and internal phase volume fraction of the emulsion templates. With increasing the g-HAp concentration or decreasing the internal phase volume fraction, the pore size and the porosity decrease, while the Young's modulus and the compressive stress enhance. Moreover, the in vitro mineralization tests show that the bioactivity of the scaffolds increases with increasing the g-HAp concentration. Furthermore, the anti-inflammatory drug ibuprofen (IBU) is loaded into the scaffolds, and the drug release studies indicate that the loaded-IBU exhibits a sustained release profile. Finally, in vitro cell culture assays prove that the scaffolds are biocompatible because of supporting adhesion, spreading, and proliferation of mouse bone mesenchymal stem cells. All the results indicate that the solvent evaporation based on Pickering HIPE templates is a promising alternative method to fabricate NC porous scaffolds for potential bone tissue engineering applications.
authors.affiliation: Research Institute of Materials Science, South China University of Technology , Guangzhou 510640, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Hu
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Gu
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.forename: Meng
authors.initials: M
authors.lastname: Hu
authors.forename: Weike
authors.initials: W
authors.lastname: Chen
authors.forename: Zhen
authors.initials: Z
authors.lastname: Tong
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001842:Bone and Bones / Q000166:cytology; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry*; D004655:Emulsions; D007052:Ibuprofen / Q000494:pharmacology; D019344:Lactic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008903:Minerals / Q000737:chemistry; D008970:Molecular Weight; D053761:Nanocomposites / Q000737:chemistry / Q000648:ultrastructure; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D004655:Emulsions; D008903:Minerals; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D017886:Durapatite; D007052:Ibuprofen
keywords: Pickering high internal phase emulsions; hydroxyapatite; poly(lactide-co-glycotide); porous scaffold; solvent evaporation