title: Assessment of tumor cells in a mouse model of diffuse infiltrative glioma by Raman spectroscopy.
abstract: Glioma of infiltrative nature is challenging for surgeons to achieve tumor-specific and maximal resection. Raman spectroscopy provides structural information on the targeted materials as vibrational shifts. We utilized Raman spectroscopy to distinguish invasive tumors from normal tissues. Spectra obtained from replication-competent avian sarcoma-(RCAS-) based infiltrative glioma cells and glioma tissues (resembling low-grade human glioma) were compared with those obtained from normal mouse astrocytes and normal tissues. In cell analysis, the spectra at 950-1000, 1030, 1050-1100, 1120-1130, 1120-1200, 1200-1300, 1300-1350, and 1450 cm(-1) were significantly higher in infiltrative glioma cells than in normal astrocytes. In brain tissue analysis, the spectra at 1030, 1050-1100, and 1200-1300 cm(-1) were significantly higher in infiltrative glioma tissues than in normal brain tissues. These spectra reflect the structures of proteins, lipids, and DNA content. The sensitivity and specificity to predict glioma cells by distinguishing normal cells were 98.3% and 75.0%, respectively. Principal component analysis elucidated the significance of spectral difference between tumor tissues and normal tissues. It is possible to distinguish invasive tumors from normal tissues by using Raman spectroscopy.
authors.affiliation: Department of Neurosurgery, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Tanahashi
authors.affiliation: Department of Neurosurgery, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Natsume
authors.affiliation: Department of Neurosurgery, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan ; Division of Epigenomics and Molecular Oncology, Aichi Cancer Center Research Institute, Japan.
authors.forename: Fumiharu
authors.identifier: 0000-0002-5569-5626
authors.initials: F
authors.lastname: Ohka
authors.affiliation: Department of Neurosurgery, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
authors.forename: Hiroyuki
authors.identifier: 0000-0002-0154-2798
authors.initials: H
authors.lastname: Momota
authors.affiliation: Department of Neurosurgery, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
authors.forename: Akira
authors.identifier: 0000-0002-2696-5703
authors.initials: A
authors.lastname: Kato
authors.affiliation: Department of Neurosurgery, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Motomura
authors.affiliation: Renishaw, Raman Group, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Watabe
authors.affiliation: Renishaw, Raman Group, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Muraishi
authors.affiliation: Department of Quantum Engineering, Nagoya University School of Engineering, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nakahara
authors.affiliation: Department of Quantum Engineering, Nagoya University School of Engineering, Japan.
authors.forename: Yahachi
authors.initials: Y
authors.lastname: Saito
authors.affiliation: Department of Computer Science/Scientific and Engineering Simulation, Nagoya Institute of Technology, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takeuchi
authors.affiliation: Department of Neurosurgery, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Wakabayashi
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000737:chemistry*; D001932:Brain Neoplasms / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D005910:Glioma / Q000737:chemistry*; D008055:Lipids / Q000032:analysis*; D051379:Mice; D008813:Mice, Inbred ICR; D009363:Neoplasm Proteins / Q000032:analysis*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014407:Tumor Cells, Cultured
chemical_list: D008055:Lipids; D009363:Neoplasm Proteins; D004247:DNA