title: Biochemical characterization of a malonyl-specific acyltransferase domain of FK506 biosynthetic polyketide synthase.
abstract: Acyltransferases (ATs) play an essential role in the polyketide biosynthesis through transferring acyl units into acyl carrier proteins (ACPs) via a self-acylation reaction and a transacylation reaction. Here we used AT10FkbA of FK506 biosynthetic polyketide synthase (PKS) from Streptomyces tsukubaensis YN06 as a model to study the specificity of ATs for acyl units. Our results show that AT10FkbA can form both malonyl-O-AT10FkbA and methylmalonyl-O-AT10FkbA in the self-acylation reaction, however, only malonyl-O-AT10FkbA but not methylmalonyl-O-AT10FkbA can transfer the acyl unit into ACPs in the transacylation reaction. Unlike some ATs that are known to control the acyl specificity in self-acylation reactions, AT10FkbA controls the acyl specificity in transacylation reactions.
authors.forename: Yue-Yue
authors.initials: YY
authors.lastname: Wang
authors.forename: Long-Fei
authors.initials: LF
authors.lastname: Bai
authors.forename: Xin-Xin
authors.initials: XX
authors.lastname: Ran
authors.forename: Xin-Hang
authors.initials: XH
authors.lastname: Jiang
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Mei-Ying
authors.initials: MY
authors.lastname: Jin
authors.forename: Yong-Quan
authors.initials: YQ
authors.lastname: Li
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, China. jianghuisioc@zju.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000213:Acyl Carrier Protein / Q000096:biosynthesis* / Q000737:chemistry; D000215:Acylation; D000217:Acyltransferases / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D008316:Malonyl Coenzyme A; D009097:Multienzyme Complexes; D048630:Polyketide Synthases / Q000737:chemistry / Q000378:metabolism*; D061065:Polyketides / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D013302:Streptomyces / Q000201:enzymology; D013379:Substrate Specificity; D016559:Tacrolimus / Q000737:chemistry
chemical_list: D000213:Acyl Carrier Protein; D009097:Multienzyme Complexes; D061065:Polyketides; D008316:Malonyl Coenzyme A; D048630:Polyketide Synthases; D000217:Acyltransferases; D016559:Tacrolimus