title: Transforming growth factor-β (TGF-β) expression is increased in the subsynovial connective tissue in a rabbit model of carpal tunnel syndrome.
abstract: Carpal tunnel syndrome (CTS) is an idiopathic disease that results from increased fibrosis of the subsynovial connective tissue (SSCT). A recent study found overexpression of both transforming growth factor-β (TGF-β) and connective tissue growth factor (CTGF) in the SSCT of CTS patients. This study investigated TGF-β and CTGF expression in a rabbit model of CTS, in which SSCT fibrosis is induced by a surgical injury. Levels of TGF-β1 and CTGF at 6, 12, 24 weeks after injury were determined by immunohistochemistry A significant increase in TGF-β1 and a concomitant significant increase in CTGF were found at 6 weeks, in addition to higher cell density compared to normal (all p<0.05), Interestingly, CTGF expression was reduced at 12 and 24 weeks, suggesting that an initial insult results in a time limited response. We conclude that this rabbit model mimics the fibrosis found in human CTS, and may be useful to study pathogenetic mechanisms of CTS in vivo.
authors.affiliation: Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, United States of America.
authors.forename: Takako
authors.initials: T
authors.lastname: Chikenji
authors.affiliation: Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota, United States of America.
authors.forename: Anne
authors.initials: A
authors.lastname: Gingery
authors.affiliation: Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, United States of America.
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, United States of America.
authors.forename: Matthias
authors.initials: M
authors.lastname: Vanhees
authors.affiliation: Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, United States of America.
authors.forename: Tamami
authors.initials: T
authors.lastname: Moriya
authors.affiliation: Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, United States of America.
authors.forename: Ramona
authors.initials: R
authors.lastname: Reisdorf
authors.affiliation: Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, United States of America.
authors.forename: Kai-Nan
authors.initials: KN
authors.lastname: An
authors.affiliation: Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, United States of America.
authors.forename: Peter C
authors.initials: PC
authors.lastname: Amadio
mesh_terms: D000818:Animals; D002349:Carpal Tunnel Syndrome / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002452:Cell Count; D003238:Connective Tissue / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D055513:Connective Tissue Growth Factor / Q000235:genetics* / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D005355:Fibrosis; D015870:Gene Expression; D006801:Humans; D008663:Metacarpus / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D011817:Rabbits; D053773:Transforming Growth Factor beta1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D053773:Transforming Growth Factor beta1; D055513:Connective Tissue Growth Factor