title: Next generation sequencing yields the complete mitochondrial genome of the flathead mullet, Mugil cephalus cryptic species NWP2 (Teleostei: Mugilidae).
abstract: In this study, the complete mitogenome sequence of Northwestern Pacific 2 (NWP2) cryptic species of flathead mullet, Mugil cephalus (Teleostei: Mugilidae) has been amplified by long-range PCR and sequenced by next-generation sequencing method. The assembled mitogenome, consisting of 16,686 bp, had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a non-coding control region of D-loop. D-loop was 909 bp length and was located between tRNA-Pro and tRNA-Phe. The overall base composition of NWP2 M. cephalus was 28.4% for A, 29.8% for C, 26.5% for T and 15.3% for G. The complete mitogenome may provide essential and important DNA molecular data for further phylogenetic and evolutionary analysis for flathead mullet species complex.
authors.affiliation: a Department of Environmental Biology and Fisheries Science , National Taiwan Ocean University , Keelung , Taiwan , ROC .|b Center of Excellence for the Oceans, National Taiwan Ocean University , Keelung , Taiwan , ROC .
authors.forename: Kang-Ning
authors.initials: KN
authors.lastname: Shen
authors.affiliation: c Department of Chemistry , Chung Yuan Christian University , Chung-Li , Taiwan .
authors.forename: Ta-Chi
authors.initials: TC
authors.lastname: Yen
authors.affiliation: d WeThink Biotech INC , Taoyuan , Taiwan , ROC .
authors.forename: Ching-Hung
authors.initials: CH
authors.lastname: Chen
authors.affiliation: e Department of Medical Genetics , National Taiwan University Hospital , Taipei , Taiwan , ROC , and.
authors.forename: Huei-Ying
authors.initials: HY
authors.lastname: Li
authors.affiliation: e Department of Medical Genetics , National Taiwan University Hospital , Taipei , Taiwan , ROC , and.
authors.forename: Pei-Lung
authors.initials: PL
authors.lastname: Chen
authors.affiliation: f Department of Bioscience Technology , Chung Yuan Christian University , Chung-Li , Taiwan , ROC.
authors.forename: Chung-Der
authors.initials: CD
authors.lastname: Hsiao
mesh_terms: D000818:Animals; D054629:Genome, Mitochondrial / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D023701:Smegmamorpha / Q000145:classification / Q000235:genetics*
chemical_list: D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: Flathead mullet; mitogenome; next generation sequencing