title: Identification and characterization of a late gene encoded by grouper iridovirus 2L (GIV-2L).
abstract: Grouper iridovirus (GIV) belongs to the Ranavirus genus and is one of the most important viral pathogens in grouper, particularly at the fry and fingerling stages. In this study, we identified and characterized the GIV-2L gene, which encodes a protein of unknown function. GIV-2L is 1242 bp in length, with a predicted protein mass of 46.2 kDa. It displayed significant identity only with members of the Ranavirus and Iridovirus genera. We produced mouse monoclonal antibodies against the GIV-2L protein by immunizing mice with GIV-2L-His-tag recombinant protein. By inhibiting de novo protein and DNA synthesis in GIV-infected cells, we showed that GIV-2L was a late gene during the viral replication. Finally, immunofluorescence microscopy revealed that GIV-2L protein accumulated in both the nucleus and cytoplasm of infected cells. These results offer important insights into the pathogenesis of GIV.
authors.affiliation: Department of Biotechnology and Animal Science, National Ilan University, Yilan, Taiwan.|Department of Horticulture, National Ilan University, Yilan, Taiwan.
authors.forename: H-Y
authors.initials: HY
authors.lastname: Lin
authors.affiliation: Department of Biotechnology and Animal Science, National Ilan University, Yilan, Taiwan.
authors.forename: C-F
authors.initials: CF
authors.lastname: Cheng
authors.affiliation: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: P P
authors.initials: PP
authors.lastname: Chiou
authors.affiliation: Department of Nursing, Chang Gung University of Science and Technology, Taoyuan, Taiwan.|Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan.
authors.forename: C-J
authors.initials: CJ
authors.lastname: Liou
authors.affiliation: Department of Horticulture, National Ilan University, Yilan, Taiwan.
authors.forename: J-C
authors.initials: JC
authors.lastname: Yiu
authors.affiliation: Department of Biotechnology and Animal Science, National Ilan University, Yilan, Taiwan.
authors.forename: Y-S
authors.initials: YS
authors.lastname: Lai
keywords: grouper iridovirus; grouper iridovirus 2L; immunofluorescence; monoclonal antibodies