title: The glycoprofile patterns of endothelial cells in usual interstitial pneumonia.
abstract: BACKGROUND
  The pathological classification of cryptogenic fibrosing alveolitis has been a matter of debate and controversy for histopathologists.
  
  
  OBJECTIVE
  To identify and specify the glycotypes of capillary endothelial cells in usual interstitial pneumonia (UIP) compared to those found in normal tissue.
  
  
  METHODS
  Sections of formalin-fixed, paraffin-embedded blocks from 16 cases of UIP were studied by lectin histochemistry with a panel of 27 biotinylated lectins and an avidin-peroxidase revealing system.
  
  
  RESULTS
  High expression of several classes of glycan was seen de novo in capillary endothelial cells from patients with UIP including small complex and bi/tri-antennary bisected complex N-linked sequences bolund by Concanavalin A and erythro-phytohemagglutinin, respectively, GalNAca1 residues bound by Helix pomatia and Maclura pomifera agglutinins, and L-fucosylated derivatives of type II glycan chains recognized by Ulex europaeus agglutinin-I. Glycans bound by agglutinins from Lycopersicon esculentum (β1,4GlcNAc) and Wisteria floribunda (GalNAc) as well as GlcNAc oligomers bound by Phytolacca americana and succinylated Wheat Germ agglutinin were also seen in the capillary endothelial cells of UIP. In contrast, L-fucosylated derivatives of type I glycan chains were absent in cells from cases of UIP when Anguilla anguilla agglutinin was applied, unlike the situation in normal tissue.
  
  
  CONCLUSIONS
  These results may indicate existence of two distinct populations of endothelial cell in UIP with markedly different patterns of glycosylation, reflecting a pattern of differentiation and angiogenesis, which is not detectable morphologically.
authors.forename: A
authors.initials: A
authors.lastname: Barkhordari
authors.forename: C Jp
authors.initials: CJ
authors.lastname: Jones
authors.forename: R W
authors.initials: RW
authors.lastname: Stoddart
authors.forename: S F
authors.initials: SF
authors.lastname: McClure
authors.forename: J
authors.initials: J
authors.lastname: McClure
authors.affiliation: Occupational Health Department, Neyshabur University of Medical Sciences, Neyshabur, Iran. s.rahimimoghadam@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Rahimi Moghadam