title: Bone marrow stromal cell-derived extracellular matrix promotes osteogenesis of adipose-derived stem cells.
abstract: Adipose-derived stem cells (ASCs) can differentiate into multiple cell lineages and favor adipogenesis rather than osteogenesis. Because the extracellular matrix (ECM) component of the stem cell niche is important in stem cell differentiation, we hypothesized that ECM produced by human bone marrow stromal cells (BM-ECM) could enhance the osteogenic potential of ASCs during in vitro expansion. We have compared the replication and osteogenic differentiation of ASCs expanded on BM-ECM versus tissue culture plastic (TCP) in vitro and in vivo. During the first two passages, ASC proliferation on BM-ECM was 3.27-fold greater than that on TCP. ASCs expanded on BM-ECM formed more osteogenic colonies and higher expression of osteogenic markers than ASCs expanded on TCP. In nude mice, ASCs that had been expanded on BM-ECM formed more new bone tissue than those expanded on TCP. The data indicate that BM-ECM can be used to promote the osteogenic fate of ASCs.
authors.affiliation: Department of Plastic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, P.R. China.
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xuan
authors.initials: X
authors.lastname: Luo
authors.forename: Haisong
authors.initials: H
authors.lastname: Xu
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Ren
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
authors.forename: Mingang
authors.initials: M
authors.lastname: Fu
authors.forename: Yixiong
authors.initials: Y
authors.lastname: Huang
authors.forename: Jiyin
authors.initials: J
authors.lastname: He
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Fan
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D017886:Durapatite / Q000737:chemistry; D005109:Extracellular Matrix / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D015675:Osteocalcin; D017886:Durapatite
keywords: adipose-derived stem cells; bone marrow-derived extracellular matrix; osteogenesis; proliferation