title: Knockdown of CETN1 inhibits breast cancer cells proliferation.
abstract: OBJECTIVE
  Breast cancer is a multifactorial disease and identification of the genes and proteins regulating breast carcinogenesis is critical in exploring novel targeted therapies. The aim of this study was to investigate the role CETN1 in breast cancer growth and metastasis.
  
  
  METHODS
  CETN1 expression levels were measured in breast tissue samples by immunohistochemistry (IHC). We selected high CETN1-expressing cell lines from human breast carcinoma cells with metastatic potential. The proliferation of cells was evaluated by MTS assay. Changes in progression of cell division were assessed by cell cycle analysis. Changes in CETN1 levels in breast tumor cells were analysed by Western blot.
  
  
  RESULTS
  Our results demonstrated that overexpression of CETN1 occurred mainly in breast cancer cells in which CETN1 expression was low. Knockdown of CETN1 by lentivirus- shRNA significantly inhibited breast cancer cells' proliferation, growth and metastasis.
  
  
  CONCLUSIONS
  CETN1 is overexpressed in breast cancer tissue and promotes cells'proliferation, tumor growth and metastasis.
authors.affiliation: Oncology Department, Fujian, Hong Kong, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Shuangta
authors.forename: He
authors.initials: H
authors.lastname: Yali
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhengrong
authors.forename: Zhao
authors.initials: Z
authors.lastname: Qingquan
authors.forename: Zeng
authors.initials: Z
authors.lastname: Xiaoshan
authors.forename: Yao
authors.initials: Y
authors.lastname: Fanghui
authors.forename: Waihung
authors.initials: W
authors.lastname: Ng
authors.forename: Liang
authors.initials: L
authors.lastname: Yinghui
authors.forename: Xu
authors.initials: X
authors.lastname: Jianhua
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology; D002135:Calcium-Binding Proteins / Q000502:physiology*; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000502:physiology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D051379:Mice; D034622:RNA Interference; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: C580665:CETN1 protein, human; D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins