title: Use of a gelatin hydrogel membrane containing β-tricalcium phosphate for guided bone regeneration enhances rapid bone formation.
abstract: The purpose of this study was to evaluate a thin gelatin hydrogel membrane containing β-tricalcium phosphate (G-TCP) for use in guided bone regeneration, a technique that we developed. G-TCP membranes were fabricated from gelatin and β-TCP powder, freezedried, and cross-linked by heating. The resulting G-TCP membranes were as thin as collagen membranes, with high mechanical integrity. Proliferation and differentiation of rat bone marrow stromal cells (BMSCs) on G-TCP and collagen membranes were examined. On both membranes, BMSCs proliferated well and expressed alkaline phosphatase. However, more bone-like mineralized tissue formed on G-TCP membranes than on collagen membranes at 4 weeks. The effects of G-TCP and collagen membranes on bone regeneration in rat parietal bone defects were histologically examined. Bone bridges with mature uniform bone were observed under G-TCP membranes as early as 2 weeks. These results indicate that G-TCP is a GBR membrane that is comparable or superior to collagen membrane.
authors.affiliation: Section of Oral Implantology and Regenerative Dental Medicine, Department of Masticatory Function Rehabilitation, Tokyo Medical and Dental University.
authors.forename: Kanako
authors.initials: K
authors.lastname: Noritake
authors.forename: Shinji
authors.initials: S
authors.lastname: Kuroda
authors.forename: Myat
authors.initials: M
authors.lastname: Nyan
authors.forename: Yuji
authors.initials: Y
authors.lastname: Atsuzawa
authors.forename: Motohiro
authors.initials: M
authors.lastname: Uo
authors.forename: Keiichi
authors.initials: K
authors.lastname: Ohya
authors.forename: Shohei
authors.initials: S
authors.lastname: Kasugai
mesh_terms: D001846:Bone Development; D001861:Bone Regeneration; D002130:Calcium Phosphates / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D020100:Hydrogels; D008855:Microscopy, Electron, Scanning
chemical_list: D002130:Calcium Phosphates; D020100:Hydrogels; C485817:beta-tricalcium phosphate; D005780:Gelatin