title: Identification of differentially expressed genes in breast muscle and skin fat of postnatal Pekin duck.
abstract: Lean-type Pekin duck is a commercial breed that has been obtained through long-term selection. Investigation of the differentially expressed genes in breast muscle and skin fat at different developmental stages will contribute to a comprehensive understanding of the potential mechanisms underlying the lean-type Pekin duck phenotype. In the present study, RNA-seq was performed on breast muscle and skin fat at 2-, 4- and 6-weeks of age. More than 89% of the annotated duck genes were covered by our RNA-seq dataset. Thousands of differentially expressed genes, including many important genes involved in the regulation of muscle development and fat deposition, were detected through comparison of the expression levels in the muscle and skin fat of the same time point, or the same tissue at different time points. KEGG pathway analysis showed that the differentially expressed genes clustered significantly in many muscle development and fat deposition related pathways such as MAPK signaling pathway, PPAR signaling pathway, Calcium signaling pathway, Fat digestion and absorption, and TGF-beta signaling pathway. The results presented here could provide a basis for further investigation of the mechanisms involved in muscle development and fat deposition in Pekin duck.
authors.affiliation: Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, P.R. China; Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Science, Danzhuo, Hainan, P.R. China.
authors.forename: Tieshan
authors.initials: T
authors.lastname: Xu
authors.affiliation: Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, P.R. China; Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Gu
authors.affiliation: Department of Animal Science, University of Illinois, Urbana, Illinois, United States of America.
authors.forename: Kyle Michael
authors.initials: KM
authors.lastname: Schachtschneider
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Xie
authors.affiliation: Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, P.R. China.
authors.forename: Shuisheng
authors.initials: S
authors.lastname: Hou
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004372:Ducks; D020869:Gene Expression Profiling; D018482:Muscle, Skeletal / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D012867:Skin / Q000033:anatomy & histology*
chemical_list: D017931:DNA Primers