title: Transplantation of SHED prevents bone loss in the early phase of ovariectomy-induced osteoporosis.
abstract: Stem cells from human exfoliated deciduous teeth (SHED) are a unique postnatal stem cell population, possessing multipotent differentiation capacity and immunomodulatory properties. However, the mechanism by which SHED treat immune diseases is not fully understood. Here we show that systemic transplantation of SHED via the tail vein ameliorates ovariectomy (OVX)-induced osteopenia by reducing T-helper 1 (Th1) and T-helper 17 (Th17) cell numbers in the recipient OVX mice. Mechanistically, SHED transplantation induces activated T-cell apoptosis in OVX mice via Fas ligand (FasL)-mediated Fas pathway activation, leading to up-regulation of regulatory T-cells (Tregs) and down-regulation of Th1 and Th17 cells. This SHED-mediated immunomodulation rescues OVX-induced impairment of bone marrow mesenchymal stem cells (BMMSCs) and activation of osteoclastogenesis, resulting in increased bone mass. In summary, SHED-mediated T-cell apoptosis via a FasL/Fas pathway results in immune tolerance and ameliorates the osteopenia phenotype in OVX mice.
authors.affiliation: Center for Craniofacial Molecular Biology, Ostrow School of Dentistry, University of Southern California, 2250 Alcazar Street, CSA 103, Los Angeles, CA 90033, USA Department of Pediatric Dentistry, School of Stomatology, China Medical University, Shenyang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Center for Craniofacial Molecular Biology, Ostrow School of Dentistry, University of Southern California, 2250 Alcazar Street, CSA 103, Los Angeles, CA 90033, USA.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.affiliation: Center for Craniofacial Molecular Biology, Ostrow School of Dentistry, University of Southern California, 2250 Alcazar Street, CSA 103, Los Angeles, CA 90033, USA.
authors.forename: S
authors.initials: S
authors.lastname: Liu
authors.affiliation: Center for Craniofacial Molecular Biology, Ostrow School of Dentistry, University of Southern California, 2250 Alcazar Street, CSA 103, Los Angeles, CA 90033, USA Department of Anatomy and Cell Biology, University of Pennsylvania, School of Dental Medicine, Philadelphia, PA 19104, USA.
authors.forename: D
authors.initials: D
authors.lastname: Liu
authors.affiliation: Center for Craniofacial Molecular Biology, Ostrow School of Dentistry, University of Southern California, 2250 Alcazar Street, CSA 103, Los Angeles, CA 90033, USA Department of Anatomy and Cell Biology, University of Pennsylvania, School of Dental Medicine, Philadelphia, PA 19104, USA.
authors.forename: C
authors.initials: C
authors.lastname: Chen
authors.affiliation: Center for Craniofacial Molecular Biology, Ostrow School of Dentistry, University of Southern California, 2250 Alcazar Street, CSA 103, Los Angeles, CA 90033, USA.
authors.forename: X
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Pediatric Dentistry, School of Stomatology, China Medical University, Shenyang, China.
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.affiliation: Center for Craniofacial Molecular Biology, Ostrow School of Dentistry, University of Southern California, 2250 Alcazar Street, CSA 103, Los Angeles, CA 90033, USA Department of Anatomy and Cell Biology, University of Pennsylvania, School of Dental Medicine, Philadelphia, PA 19104, USA songtaos@usc.edu.
authors.forename: S
authors.initials: S
authors.lastname: Shi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001851:Bone Diseases, Metabolic / Q000276:immunology / Q000517:prevention & control; D053222:Fas Ligand Protein / Q000502:physiology; D005260:Female; D006801:Humans; D007108:Immune Tolerance / Q000276:immunology; D018655:Lymphocyte Count; D059630:Mesenchymal Stem Cells / Q000502:physiology; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D010010:Osteoclasts / Q000502:physiology; D010024:Osteoporosis / Q000276:immunology / Q000517:prevention & control*; D010052:Ovariectomy; D033581:Stem Cell Transplantation / Q000379:methods*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000473:pathology; D018417:Th1 Cells / Q000276:immunology / Q000473:pathology; D058504:Th17 Cells / Q000276:immunology / Q000473:pathology; D014094:Tooth, Deciduous / Q000166:cytology*; D019014:fas Receptor / Q000502:physiology
chemical_list: D053222:Fas Ligand Protein; C491899:Fas protein, mouse; D019014:fas Receptor
keywords: Fas ligand; apoptosis; deciduous teeth; immunotherapy; mesenchymal stem cells; regulatory T cells (Tregs)