title: Evaluation of biofilm of Aeromonas hydrophila for oral vaccination of Channa striatus.
abstract: Our laboratory has developed a biofilm oral vaccine of Aeromonas hydrophila, which has given significantly higher antibody agglutination titre and protection in herbivorous carps and omnivorous walking catfish compared to that with free cell vaccine. Against this background, in the present study A. hydrophila biofilm oral vaccine was evaluated in Channa striatus, a carnivorous fish model. The fish was fed with biofilm (BF) and free cell (FC) of A. hydrophila vaccine at 10(10) cells/g fish/day for 20 d. Serum antibody production monitored with a monoclonal antibody based ELISA for 60 day post vaccination. Significantly higher antibody titre was recorded with BF compared to that with FC. Furthermore, BF vaccinated fish upon challenge with A. hydrophila at 10(9) cfu/ml had significantly higher relative per cent survival (88) than that with FC (29.6).
authors.affiliation: Aquatic Health Management Laboratory, Department of Aquaculture, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Mangalore 575002, Karnataka, India.
authors.forename: Prabhugouda
authors.initials: P
authors.lastname: Siriyappagouder
authors.affiliation: Aquatic Health Management Laboratory, Department of Aquaculture, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Mangalore 575002, Karnataka, India. Electronic address: kalkulishankar@gmail.com.
authors.forename: K M
authors.initials: KM
authors.lastname: Shankar
authors.affiliation: Aquatic Health Management Laboratory, Department of Aquaculture, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Mangalore 575002, Karnataka, India.
authors.forename: B T
authors.initials: BT
authors.lastname: Naveen Kumar
authors.affiliation: Aquatic Health Management Laboratory, Department of Aquaculture, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Mangalore 575002, Karnataka, India.
authors.forename: Rajreddy
authors.initials: R
authors.lastname: Patil
authors.affiliation: Aquatic Health Management Laboratory, Department of Aquaculture, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Mangalore 575002, Karnataka, India.
authors.forename: Omkar V
authors.initials: OV
authors.lastname: Byadgi
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D017756:Aquaculture / Q000379:methods*; D018441:Biofilms; D002397:Catfishes / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005393:Fish Diseases / Q000382:microbiology* / Q000517:prevention & control*; D016905:Gram-Negative Bacterial Infections / Q000517:prevention & control / Q000662:veterinary*; D007928:Lethal Dose 50; D010473:Perciformes / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial
keywords: Aeromonas hydrophila; Biofilm vaccine; Channa striatus