title: Depolymerized glycosaminoglycan and its anticoagulant activities from sea cucumber Apostichopus japonicus.
abstract: A controlled Cu(2+) catalytic free-radical depolymerization process of fucosylated chondroitin sulfate from sea cucumber Apostichopus japonicus was established. The results showed a good linear relationship between 1/Mw and time during the depolymerization. A series of fractions with different molecular weight were obtained, and the physicochemical properties of them were investigated and compared utilizing the chemical method, IR spectra and NMR spectra. The results showed no significant variations of the backbone and branches structures during the depolymerization. Furthermore, the anticoagulant activities of the depolymerized fractions were evaluated by the activated partial thromboplastin time (APTT). The APTT decreases in proportion to the molecular weight following a linear relationship and the prolongation of APTT activity requires at least oligosaccharide of 4 trisaccharide units (about 4000 Da). Their anticoagulant activity of low molecular weight fraction (Mw = 24,755 Da) is similar to LMWH with significantly less bleeding risk. The results suggest that the low molecular weight fraction could be used as a novel anticoagulant with less undesired side effects.
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Shandong Provincial Key Laboratory of Glycoscience & Glycotechnology (Ocean University of China), Qingdao 266003, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Shandong Provincial Key Laboratory of Glycoscience & Glycotechnology (Ocean University of China), Qingdao 266003, China.
authors.forename: Yuanhong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Shandong Provincial Key Laboratory of Glycoscience & Glycotechnology (Ocean University of China), Qingdao 266003, China.
authors.forename: Tingfu
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Shandong Provincial Key Laboratory of Glycoscience & Glycotechnology (Ocean University of China), Qingdao 266003, China.
authors.forename: Lv
authors.initials: L
authors.lastname: Lv
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Shandong Provincial Key Laboratory of Glycoscience & Glycotechnology (Ocean University of China), Qingdao 266003, China.
authors.forename: Boyuan
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Shandong Provincial Key Laboratory of Glycoscience & Glycotechnology (Ocean University of China), Qingdao 266003, China. Electronic address: lvzhihua@ouc.edu.cn.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Lv
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects; D002384:Catalysis; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D003300:Copper / Q000737:chemistry; D006025:Glycosaminoglycans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D010314:Partial Thromboplastin Time; D058105:Polymerization; D012616:Sea Cucumbers / Q000737:chemistry; D012756:Sheep / Q000097:blood; D013917:Thrombin / Q000737:chemistry
chemical_list: D000925:Anticoagulants; D006025:Glycosaminoglycans; C517150:fucosylated chondroitin sulfate; D003300:Copper; D002809:Chondroitin Sulfates; D013917:Thrombin
keywords: Apostichopus japonicus; Free-radical depolymerization; Fucosylated chondroitin sulfate