title: Characterization of membrane lipidome changes in Pseudomonas aeruginosa during biofilm growth on glass wool.
abstract: Bacteria cells within biofilms are physiologically distinct from their planktonic counterparts. In particular they are more resistant to detrimental environmental conditions. In this study, we monitored the evolution of the phospholipid composition of the inner and outer membranes of P. aeruginosa during the biofilm formation (i.e., from 1-, 2-, to 6-day-old biofilm). Lipidome analyses were performed by electrospray ionization mass spectrometry. In addition to the lipidomic analysis, the fatty acid composition was analysed by gas chromatography/mass spectrometry. We found that the lipidome alterations of the inner and the outer membranes varied with the biofilm age. These alterations in phospholipid compositions reflect a higher diversity in sessile organisms than in planktonic counterparts. The diversity is characterized by the presence of PE 30∶1, PE 31∶0 and PG 31∶0 for the lower masses as well as PE 38∶1, 38∶2, 39∶1, 39∶2 and PG 38∶0, 38∶1, 38∶2, 39∶1, 39∶2 for the higher masses. However, this lipidomic feature tends to disappear with the biofilm age, in particular the high mass phospholipids tend to disappear. The amount of branched chains phospholipids mainly located in the outer membrane decreased with the biofilm age, whereas the proportion of cyclopropylated phospholipids increased in both membranes. In bacteria present in oldest biofilms, i.e., 6-day-old, the phospholipid distribution moved closer to that of planktonic bacteria.
authors.affiliation: Polymères Biopolymères, Surfaces Laboratory - UMR 6270, CNRS - Université de Rouen, Mont-Saint-Aignan, France; Normandie Université, Mont-Saint-Aignan, France.
authors.forename: Hayette
authors.initials: H
authors.lastname: Benamara
authors.affiliation: Polymères Biopolymères, Surfaces Laboratory - UMR 6270, CNRS - Université de Rouen, Mont-Saint-Aignan, France; Normandie Université, Mont-Saint-Aignan, France; PISSARO proteomic facility, IRIB - Université de Rouen, Mont-Saint-Aignan, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Rihouey
authors.affiliation: Polymères Biopolymères, Surfaces Laboratory - UMR 6270, CNRS - Université de Rouen, Mont-Saint-Aignan, France; Normandie Université, Mont-Saint-Aignan, France.
authors.forename: Imen
authors.initials: I
authors.lastname: Abbes
authors.affiliation: Polymères Biopolymères, Surfaces Laboratory - UMR 6270, CNRS - Université de Rouen, Mont-Saint-Aignan, France; Normandie Université, Mont-Saint-Aignan, France; PISSARO proteomic facility, IRIB - Université de Rouen, Mont-Saint-Aignan, France.
authors.forename: Mohamed Amine
authors.initials: MA
authors.lastname: Ben Mlouka
authors.affiliation: Polymères Biopolymères, Surfaces Laboratory - UMR 6270, CNRS - Université de Rouen, Mont-Saint-Aignan, France; Normandie Université, Mont-Saint-Aignan, France; PISSARO proteomic facility, IRIB - Université de Rouen, Mont-Saint-Aignan, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Hardouin
authors.affiliation: Polymères Biopolymères, Surfaces Laboratory - UMR 6270, CNRS - Université de Rouen, Mont-Saint-Aignan, France; Normandie Université, Mont-Saint-Aignan, France; PISSARO proteomic facility, IRIB - Université de Rouen, Mont-Saint-Aignan, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Jouenne
authors.affiliation: Polymères Biopolymères, Surfaces Laboratory - UMR 6270, CNRS - Université de Rouen, Mont-Saint-Aignan, France; Normandie Université, Mont-Saint-Aignan, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Alexandre
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development*; D002462:Cell Membrane / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D005898:Glass; D010714:Phosphatidylethanolamines / Q000032:analysis; D010715:Phosphatidylglycerols / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000502:physiology*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; C483858:phosphatidylethanolamine