title: Expression of programmed death 1 is correlated with progression of osteosarcoma.
abstract: Accumulating bodies of evidence indicate that immune dysregulation plays a key role in the development of osteosarcoma (OS). Programmed death 1 (PD-1) is a surface receptor expressed on activated and exhausted T cells, which mediate T-cell inhibition upon binding with its ligand. Researches on PD-1 and OS remain extremely limited. Here, we investigated whether PD-1 could be involved in the development of OS. Expression of PD-1 was measured by flow cytometry on peripheral CD4+ and CD8+ T cells from 56 OS cases and 42 healthy controls. Data revealed that percentages of PD-1 were significantly upregulated on both peripheral CD4+ and CD8+ T cells from OS patients (p < 0.001 and p < 0.001, respectively). Patients with different tumor locations did not present obvious variations in PD-1 level. However, patients with metastasis showed significantly higher level of PD-1 on CD4+ T cells than those without metastasis (p < 0.001). Furthermore, PD-1 expression on CD4+ T cells started to increase in stage III, whereas PD-1 expression on CD8+ T cells started to increase in stage II. In addition, patients with pathological fracture were observed to have elevated PD-1 on both CD4+ and CD8+ T cells. These data suggest that PD-1 is involved in the pathogenesis of OS, especially in the progression of disease.
authors.affiliation: Department of Orthopedics, Xinqiao Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zheng
authors.forename: Hong
authors.initials: H
authors.lastname: Xiao
authors.forename: Huan
authors.initials: H
authors.lastname: Liu
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000293:Adolescent; D001859:Bone Neoplasms / Q000473:pathology*; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism; D018450:Disease Progression; D005260:Female; D005269:Femur / Q000473:pathology; D050723:Fractures, Bone / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D006811:Humerus / Q000473:pathology; D008297:Male; D009367:Neoplasm Staging; D012516:Osteosarcoma / Q000473:pathology*; D061026:Programmed Cell Death 1 Receptor / Q000096:biosynthesis*; D013977:Tibia / Q000473:pathology
chemical_list: C105992:PDCD1 protein, human; D061026:Programmed Cell Death 1 Receptor
keywords: CD4; CD8; Osteosarcoma; PD-1; T cell