title: A disposable evanescent wave fiber optic sensor coated with a molecularly imprinted polymer as a selective fluorescence probe.
abstract: We have developed a disposable evanescent wave fiber optic sensor by coating a molecularly imprinted polymer (MIP) containing a fluorescent signaling group on a 4-cm long polystyrene optical waveguide. The MIP is composed of a naphthalimide-based fluorescent monomer, which shows fluorescence enhancement upon binding with carboxyl-containing molecules. The herbicide 2,4-dichlorophenoxyacetic acid and the mycotoxin citrinin were used as model analytes. The coating of the MIP was either performed ex-situ, by dip-coating the fiber with MIP particles synthesized beforehand, or in-situ by evanescent-wave photopolymerization on the fiber. The sensing element was interrogated with a fiber-coupled spectrofluorimeter. The fiber optic sensor detects targets in the low nM range and exhibits specific and selective recognition over structural analogs and non-related carboxyl-containing molecules. This technology can be extended to other carboxyl-containing analytes, and to a broader spectrum of targets using different fluorescent monomers.
authors.affiliation: CNRS Enzyme and Cell Engineering Laboratory, Compiègne University of Technology, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France.
authors.forename: Xuan-Anh
authors.initials: XA
authors.lastname: Ton
authors.affiliation: HydrISE Unit, Institut Polytechnique LaSalle Beauvais, BP 30313, 60026 Beauvais, France.
authors.forename: Victor
authors.initials: V
authors.lastname: Acha
authors.affiliation: CNRS Enzyme and Cell Engineering Laboratory, Compiègne University of Technology, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France.
authors.forename: Paolo
authors.initials: P
authors.lastname: Bonomi
authors.affiliation: CNRS Enzyme and Cell Engineering Laboratory, Compiègne University of Technology, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France. Electronic address: jeanne.tse-sum-bui@utc.fr.
authors.forename: Bernadette
authors.initials: B
authors.lastname: Tse Sum Bui
authors.affiliation: CNRS Enzyme and Cell Engineering Laboratory, Compiègne University of Technology, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France. Electronic address: karsten.haupt@utc.fr.
authors.forename: Karsten
authors.initials: K
authors.lastname: Haupt
mesh_terms: D015374:Biosensing Techniques; D005336:Fiber Optic Technology; D005453:Fluorescence; D006540:Herbicides / Q000302:isolation & purification*; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*
chemical_list: D006540:Herbicides; D011108:Polymers
keywords: Evanescent wave fiber optic sensor; Fluorescence; Molecularly imprinted polymer; Mycotoxin; Signaling monomer