title: L protease from foot and mouth disease virus confers eIF2-independent translation for mRNAs bearing picornavirus IRES.
abstract: The leader protease (L(pro)) from foot-and-mouth disease virus (FMDV) has the ability to cleave eIF4G, leading to a blockade of cellular protein synthesis. In contrast to previous reports, our present findings demonstrate that FMDV L(pro) is able to increase translation driven by FMDV IRES. Additionally, inactivation of eIF2 subsequent to phosphorylation induced by arsenite or thapsigargin in BHK cells blocks protein synthesis directed by FMDV IRES, whereas in the presence of L(pro), significant translation is found under these conditions. This phenomenon was also observed in cell-free systems after induction of eIF2 phosphorylation by addition of poly(I:C).
authors.affiliation: Centro de Biología Molecular Severo Ochoa, (CSIC-UAM), C/Nicolás Cabrera, 1, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Moral-López
authors.affiliation: Centro de Biología Molecular Severo Ochoa, (CSIC-UAM), C/Nicolás Cabrera, 1, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Alvarez
authors.affiliation: Centro de Biología Molecular Severo Ochoa, (CSIC-UAM), C/Nicolás Cabrera, 1, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Redondo
authors.affiliation: Max F. Perutz Laboratories, Medical University of Vienna, Vienna, Austria.
authors.forename: Tim
authors.initials: T
authors.lastname: Skern
authors.affiliation: Centro de Biología Molecular Severo Ochoa, (CSIC-UAM), C/Nicolás Cabrera, 1, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain. Electronic address: lcarrasco@cbm.csic.es.
authors.forename: Luis
authors.initials: L
authors.lastname: Carrasco
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D010450:Endopeptidases / Q000378:metabolism*; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism; D029544:Foot-and-Mouth Disease Virus / Q000201:enzymology* / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D015852:Eukaryotic Initiation Factor-2; D012333:RNA, Messenger; D010450:Endopeptidases; C088747:leader proteinase, foot-and-mouth disease virus
keywords: FMDV L protease; Picornavirus IRES; Poliovirus 2A; Regulation of translation; eIF2