title: A multiplex fluorescent in situ hybridization protocol for clonal analysis of Drosophila oogenesis.
abstract: Fluorescent in situ hybridization (FISH) is a common inmunohistochemical method used to examine the distribution of RNAs in tissue samples. In mosaic tissues composed of a mixed population of wild-type and loss-of- or gain-of-function mutant cells, FISH allows comparison of the effect of the perturbation on gene expression patterns in a mutant cell and its wild-type neighbors. Here, we provide a protocol for the detection of RNA in Drosophila mosaic follicular epithelia, where the mosaic analysis with a repressible cell marker (MARCM) technique is used for expression of transgenes.
authors.affiliation: Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ, USA.
authors.forename: Lily S
authors.initials: LS
authors.lastname: Cheung
authors.forename: Stanislav
authors.initials: S
authors.lastname: Shvartsman
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D002999:Clone Cells; D004331:Drosophila melanogaster / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D009866:Oogenesis; D010053:Ovary / Q000378:metabolism; D015347:RNA Probes / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: D000906:Antibodies; D015347:RNA Probes