title: Quantitative profiling of oxylipins through comprehensive LC-MS/MS analysis of Fusarium verticillioides and maize kernels.
abstract: Fusarium verticillioides is one of the most important fungal pathogens causing ear and stalk rot in maize, even if frequently asymptomatic, producing a harmful series of compounds named fumonisins. Plant and fungal oxylipins play a crucial role in determining the outcome of the interaction between the pathogen and its host. Moreover, oxylipins result as signals able to modulate the secondary metabolism in fungi. In keeping with this, a novel, quantitative LC-MS/MS method was designed to quantify up to 17 different oxylipins produced by F. verticillioides and maize kernels. By applying this method, we were able to quantify oxylipin production in vitro - F. verticillioides grown into Czapek-Dox/yeast extract medium amended with 0.2% w/v of cracked maize - and in vivo, i.e. during its growth on detached mature maize ears. This study pinpoints the role of oxylipins in a plant pathogen such as F. verticillioides and sets up a novel tool aimed at understanding the role oxylipins play in mycotoxigenic pathogens during their interactions with respective hosts.
authors.affiliation: a Dipartimento di Biologia Ambientale , Università Sapienza , Rome , Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Ludovici
authors.forename: Cristiano
authors.initials: C
authors.lastname: Ialongo
authors.forename: Massimo
authors.initials: M
authors.lastname: Reverberi
authors.forename: Marzia
authors.initials: M
authors.lastname: Beccaccioli
authors.forename: Marzia
authors.initials: M
authors.lastname: Scarpari
authors.forename: Valeria
authors.initials: V
authors.lastname: Scala
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination; D005513:Food Inspection / Q000379:methods*; D005670:Fusarium / Q000737:chemistry* / Q000254:growth & development; D007558:Italy; D057230:Limit of Detection; D015394:Molecular Structure; D054883:Oxylipins / Q000032:analysis* / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry* / Q000254:growth & development / Q000382:microbiology; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D003313:Zea mays / Q000737:chemistry* / Q000254:growth & development / Q000382:microbiology
chemical_list: D054883:Oxylipins; D010936:Plant Extracts
keywords: Fusarium; HPLC-MS/MS; MRM; linoleic and linolenic acid; oxylipins