title: Halo-tag mediated self-labeling of fluorescent proteins to molecular beacons for nucleic acid detection.
abstract: We report here the generation of a fluorescent protein (FP)-based dual molecular beacon (MB) system for nucleic acid detection. Halo-tag mediated conjugation was used for the site-specific decoration of MBs with two different FP fusions, thereby enabling easy detection of target sequences by fluorescence resonance energy transfer or FRET. Enhanced intracellular delivery was demonstrated by simply tethering a well-known TAT peptide sequence to the N-terminus of the fusion proteins.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, Delaware 19716, USA. wilfred@udel.edu.
authors.forename: Daniel
authors.initials: D
authors.lastname: Blackstock
authors.forename: Wilfred
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000494:pharmacology; D015374:Biosensing Techniques; D002460:Cell Line; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000494:pharmacology; D000882:Haplorhini; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000737:chemistry / Q000494:pharmacology; D009696:Nucleic Acids / Q000032:analysis*; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000737:chemistry / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; C485182:Cyan Fluorescent Protein; D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D009696:Nucleic Acids; D054322:tat Gene Products, Human Immunodeficiency Virus; C054562:yellow fluorescent protein, Bacteria; D049452:Green Fluorescent Proteins