title: csi2p modulates microtubule dynamics and organizes the bipolar spindle for chromosome segregation.
abstract: Proper chromosome segregation is of paramount importance for proper genetic inheritance. Defects in chromosome segregation can lead to aneuploidy, which is a hallmark of cancer cells. Eukaryotic chromosome segregation is accomplished by the bipolar spindle. Additional mechanisms, such as the spindle assembly checkpoint and centromere positioning, further help to ensure complete segregation fidelity. Here we present the fission yeast csi2+. csi2p localizes to the spindle poles, where it regulates mitotic microtubule dynamics, bipolar spindle formation, and subsequent chromosome segregation. csi2 deletion (csi2Δ) results in abnormally long mitotic microtubules, high rate of transient monopolar spindles, and subsequent high rate of chromosome segregation defects. Because csi2Δ has multiple phenotypes, it enables estimates of the relative contribution of the different mechanisms to the overall chromosome segregation process. Centromere positioning, microtubule dynamics, and bipolar spindle formation can all contribute to chromosome segregation. However, the major determinant of chromosome segregation defects in fission yeast may be microtubule dynamic defects.
authors.affiliation: Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104 Institut Curie-Centre National de la Recherche Scientifique, UMR 144, Paris 75005 France.
authors.forename: Judite
authors.initials: J
authors.lastname: Costa
authors.affiliation: Department of Biochemistry, University of Hong Kong, Pokfulam, Hong Kong.
authors.forename: Chuanhai
authors.initials: C
authors.lastname: Fu
authors.affiliation: Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104.
authors.forename: V Mohini
authors.initials: VM
authors.lastname: Khare
authors.affiliation: Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104 Institut Curie-Centre National de la Recherche Scientifique, UMR 144, Paris 75005 France tranp@mail.med.upenn.edu.
authors.forename: Phong T
authors.initials: PT
authors.lastname: Tran
mesh_terms: D002503:Centromere / Q000378:metabolism; D020090:Chromosome Segregation; D007700:Kinetics; D018386:Kinetochores / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*; D008938:Mitosis; D009154:Mutation; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism*; D059008:Time-Lapse Imaging
chemical_list: C000599721:Csi2 protein, S pombe; D008164:Luminescent Proteins; D008869:Microtubule-Associated Proteins; D029702:Schizosaccharomyces pombe Proteins