title: Silk-elastin-like protein polymer matrix for intraoperative delivery of an oncolytic vaccinia virus.
abstract: BACKGROUND
  Oncolytic viral efficacy may be limited by the penetration of the virus into tumors. This may be enhanced by intraoperative application of virus immediately after surgical resection.
  
  
  METHODS
  Oncolytic vaccinia virus GLV-1h68 was delivered in silk-elastin-like protein polymer (SELP) in vitro and in vivo in anaplastic thyroid carcinoma cell line 8505c in nude mice.
  
  
  RESULTS
  GLV-1h68 in SELP infected and lysed anaplastic thyroid cancer cells in vitro equally as effectively as in phosphate-buffered saline (PBS), and at 1 week retains a thousand fold greater infectious plaque-forming units. In surgical resection models of residual tumor, GLV-1h68 in SELP improves tumor control and shows increased viral β-galactosidase expression as compared to PBS.
  
  
  CONCLUSIONS
  The use of SELP matrix for intraoperative oncolytic viral delivery protects infectious viral particles from degradation, facilitates sustained viral delivery and transgene expression, and improves tumor control. Such optimization of methods of oncolytic viral delivery may enhance therapeutic outcomes.
authors.affiliation: Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York.|Department of Otorhinolaryngology, Mayo Clinic, Rochester, Minnesota.
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Price
authors.affiliation: Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York.|Department of Otolaryngology - Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology - Head and Neck Surgery, Ministry of Education, Beijing, China.
authors.forename: Pingdong
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York.
authors.forename: Chun-Hao
authors.initials: CH
authors.lastname: Chen
authors.affiliation: Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York.|Department of Otolaryngology - Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology - Head and Neck Surgery, Ministry of Education, Beijing, China.
authors.forename: Danni
authors.initials: D
authors.lastname: Wong
authors.affiliation: Department of Otolaryngology - Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology - Head and Neck Surgery, Ministry of Education, Beijing, China.
authors.forename: Zhenkun
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Genelux Corporation, San Diego Science Center, San Diego, California.|Department of Radiation Oncology, Rebecca & John Moores Comprehensive Cancer Center, University of California, San Diego, California.
authors.forename: Nanhai G
authors.initials: NG
authors.lastname: Chen
authors.affiliation: Genelux Corporation, San Diego Science Center, San Diego, California.|Department of Radiation Oncology, Rebecca & John Moores Comprehensive Cancer Center, University of California, San Diego, California.
authors.forename: Yong A
authors.initials: YA
authors.lastname: Yu
authors.affiliation: Genelux Corporation, San Diego Science Center, San Diego, California.|Department of Radiation Oncology, Rebecca & John Moores Comprehensive Cancer Center, University of California, San Diego, California.|Rudolf Virchow Center for Experimental Biomedicine, Institute for Biochemistry and Institute for Molecular Infection Biology, University of Wurzburg, Am Hubland, Wurzburg, Germany.
authors.forename: Aladar A
authors.initials: AA
authors.lastname: Szalay
authors.affiliation: Genelux Corporation, San Diego Science Center, San Diego, California.|Protein Polymer Technologies Inc., San Diego, California.
authors.forename: Joseph
authors.initials: J
authors.lastname: Cappello
authors.affiliation: Department of Surgery, City of Hope, Duarte, California.
authors.forename: Yuman
authors.initials: Y
authors.lastname: Fong
authors.affiliation: Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Wong
mesh_terms: D000818:Animals; D001704:Biopolymers; D045744:Cell Line, Tumor; D005349:Fibroins; D005353:Fibronectins; D007430:Intraoperative Care; D008819:Mice, Nude; D050130:Oncolytic Virotherapy / Q000379:methods*; D050504:Oncolytic Viruses; D011993:Recombinant Fusion Proteins; D013964:Thyroid Neoplasms / Q000276:immunology* / Q000601:surgery; D014616:Vaccinia virus; D010948:Viral Plaque Assay; D023041:Xenograft Model Antitumor Assays; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D001704:Biopolymers; D005353:Fibronectins; D011993:Recombinant Fusion Proteins; C089172:silk-like protein polymer; D005349:Fibroins; D001616:beta-Galactosidase
keywords: anaplastic thyroid carcinoma; oncolytic virus; surgery; vaccinia; viral therapy