title: Short communication: Conservation of Streptococcus uberis adhesion molecule and the sua gene in strains of Streptococcus uberis isolated from geographically diverse areas.
abstract: The objective was to identify and sequence the sua gene (GenBank no. DQ232760; http://www.ncbi.nlm.nih.gov/genbank/) and detect Streptococcus uberis adhesion molecule (SUAM) expression by Western blot using serum from naturally S. uberis-infected cows in strains of S. uberis isolated in milk from cows with mastitis from geographically diverse areas of the world. All strains evaluated yielded a 4.4-kb sua-containing PCR fragment that was subsequently sequenced. Deduced SUAM AA sequences from those S. uberis strains evaluated shared >97% identity. The pepSUAM sequence located at the N terminus of SUAM was >99% identical among strains of S. uberis. Streptococcus uberis adhesion molecule expression was detected in all strains of S. uberis tested. These results suggest that sua is ubiquitous among strains of S. uberis isolated from diverse geographic locations and that SUAM is immunogenic.
authors.affiliation: Zoetis, Veterinary Medicine Research and Development (VMRD), Global Therapeutic Research, 333 Portage Street, Kalamazoo, MI 49007.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Animal Science, The University of Tennessee, Knoxville 37996.
authors.forename: Oudessa Kerro
authors.initials: OK
authors.lastname: Dego
authors.affiliation: Department of Animal Science, The University of Tennessee, Knoxville 37996.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Chen
authors.affiliation: Zoetis, VMRD, Global Therapeutic Research, 405 Poplar Road, Parkville, Victoria 3052, Australia.
authors.forename: Eurife
authors.initials: E
authors.lastname: Abadin
authors.affiliation: Zoetis, Veterinary Medicine Research and Development (VMRD), Global Therapeutic Research, 333 Portage Street, Kalamazoo, MI 49007.
authors.forename: Shangfeng
authors.initials: S
authors.lastname: Chan
authors.affiliation: Zoetis, VMRD, Global Therapeutic Research, 405 Poplar Road, Parkville, Victoria 3052, Australia.
authors.forename: Lauren
authors.initials: L
authors.lastname: Jory
authors.affiliation: Zoetis, VMRD, Global Therapeutic Research, 405 Poplar Road, Parkville, Victoria 3052, Australia.
authors.forename: Steven
authors.initials: S
authors.lastname: Kovacevic
authors.affiliation: Department of Animal Science, The University of Tennessee, Knoxville 37996. Electronic address: ralmeida@utk.edu.
authors.forename: Raul A
authors.initials: RA
authors.lastname: Almeida
authors.affiliation: Department of Animal Science, The University of Tennessee, Knoxville 37996.
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Oliver
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D015815:Cell Adhesion Molecules; D017124:Conserved Sequence; D005260:Female; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D014644:Genetic Variation; D008892:Milk; D016133:Polymerase Chain Reaction; D013291:Streptococcus / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D015815:Cell Adhesion Molecules
keywords: Streptococcus uberis; Streptococcus uberis adhesion molecule; dairy cow; mastitis; sua gene sequencing