title: Nano-textured fluidic biochip as biological filter for selective survival of neuronal cells.
abstract: This is an innovative study to engineer biological filter to evaluate the effect of template surface structure and physiochemical properties that can be used for wide variety of applications in biological, health care as well as environmental protection. Specifically, planar silicon (Si) wafer and arrayed Si nano-tips (SiNT) templates were fabricated and coated with gold for various lengths of time to study the effect of surface charge, surface roughness, and hydrophilicity on biological activity of rat pheochromocytoma cell lines PC12. The initial growth and proliferation of PC12 cells on Si and SiNT templates showed an antipathy for the ultra-sharp SiNTs templates. In contrast, the same cells demonstrated a preferable adherence to and proliferation on planar Si templates, resulting in higher cell densities by three orders of magnitude than those on SiNT templates. It is hypothesized that SiNTs array does generate nano-fluidic effect such that the effective contact region for aqueous solution on SiNTs is lower than that on planar Si templates, thus decreasing adsorbable area for cell viability and survival. Moreover, the effect of the gold coating on cell number density was analyzed in terms of the surface roughness, zeta potential and wetting properties of the templates. It was determined that surface charge, as measured by the zeta potential, strongly correlated with the trend observed in the surface cell density, whereas no such correlation was observed for surface roughness or wetting properties in the ranges of our experiment conditions.
authors.affiliation: Research Center for Applied Sciences, Academia Sinica, Taipei, 11529, Taiwan.
authors.forename: Hsieh-Cheng
authors.initials: HC
authors.lastname: Han
authors.forename: Hung-Chun
authors.initials: HC
authors.lastname: Lo
authors.forename: Chia-Yu
authors.initials: CY
authors.lastname: Wu
authors.forename: Kuei-Hsien
authors.initials: KH
authors.lastname: Chen
authors.forename: Li-Chyong
authors.initials: LC
authors.lastname: Chen
authors.forename: Keng-Liang
authors.initials: KL
authors.lastname: Ou
authors.forename: Hossein
authors.initials: H
authors.lastname: Hosseinkhani
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005374:Filtration / Q000295:instrumentation*; D044085:Microfluidics / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D016716:PC12 Cells; D051381:Rats; D012825:Silicon / Q000494:pharmacology; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D012825:Silicon
keywords: biochip; colloidal forces; in vitro; nano-fluidic transport; nanostructure; silicon