title: Proteomics in paediatric cardiac surgery: is a personalised approach feasible?
abstract: The incidence of congenital cardiac abnormalities remains high. Paediatric patients with congenital cardiac defects often require surgery at a young age. The surgeries are often long and complex, rendering this population particularly vulnerable to the deleterious effects of cardiopulmonary bypass and cardiac surgery. The search for cardioprotective strategies is ongoing in an attempt to reduce the morbidity in this population. In the post-genomic era, it is apparent that simply determining the genomic sequences holds little diagnostic potential and means to determine progression of disease and response to treatment. The field of proteomics is expanding and application of proteomic techniques in the clinical setting holds great potential to advance our understanding of the proteomic changes involved in specific disease stages. This review will assess the application of proteomic techniques in the setting of paediatric cardiac surgery and highlight the need to obtain a clear understanding of the role of various proteins in children with cardiac conditions. The success and challenges of the available proteomic technology will be discussed as well as the future potential of proteomic methods for advancing our understanding of protein changes in children requiring cardiac surgery.
authors.affiliation: Murdoch Childrens Research Institute, Melbourne, Australia; Cardiac Surgery Unit and Cardiology, Royal Children's Hospital, Melbourne, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Australia.
authors.forename: Michele
authors.initials: M
authors.lastname: Hepponstall
authors.forename: Igor E
authors.initials: IE
authors.lastname: Konstantinov
mesh_terms: D001798:Blood Proteins / Q000378:metabolism*; D006348:Cardiac Surgical Procedures / Q000379:methods*; D002648:Child; D005240:Feasibility Studies; D006330:Heart Defects, Congenital / Q000097:blood / Q000378:metabolism / Q000601:surgery*; D006801:Humans; D057285:Precision Medicine / Q000379:methods / Q000639:trends; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods* / Q000639:trends; D015203:Reproducibility of Results
chemical_list: D001798:Blood Proteins; D020543:Proteome
keywords: Cardiac surgery; Paediatrics; Personalised