title: A comparison of physicochemical properties of sterilized chitosan hydrogel and its applicability in a canine model of periodontal regeneration.
abstract: Chitosan has previously been exploited as a scaffold in tissue engineering processes. To avoid infection, chitosan must be sterilized prior to contact with bodily fluids or blood. Previous research has shown that autoclaved chitosan solution lead to decreased molecular weight, dynamic viscosity, and rate of gelling. We prepared a thermosensitive chitosan hydrogel using autoclaved chitosan powder (121 °C, 10 min) and β-glycerophosphate (chitosan-PA/GP) and compared the physicochemical properties and biocompatibility in vitro with autoclaved chitosan solution/GP hydrogel. The chitosan-PA/GP hydrogel had a shortened gelation time, higher viscosity, increased water absorption, appropriate degradation time, porous structure, and no obvious cytotoxicity on human periodontal ligament cells. Scanning electron microscopy demonstrated that the cells exhibited a normal morphology. The chitosan-PA/GP hydrogel promoted periodontal tissue regeneration in dog class III furcation defects. The chitosan-PA/GP thermosensitive hydrogel displayed suitable physicochemical properties and biocompatibilities and represents a promising candidate as an injectable tissue engineering scaffold.
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Shengqi
authors.initials: S
authors.lastname: Zang
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Guangying
authors.initials: G
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Xinwen
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Lingxia
authors.initials: L
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, PR China. Electronic address: yznmbk@fmmu.edu.cn.
authors.forename: Qintao
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000818:Animals; D002478:Cells, Cultured; D055598:Chemical Phenomena / Q000187:drug effects; D002648:Child; D048271:Chitosan / Q000008:administration & dosage / Q000737:chemistry*; D004285:Dogs; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000008:administration & dosage / Q000737:chemistry*; D008297:Male; D023421:Models, Animal; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan
keywords: Chitosan; Periodontal tissue engineering; Scaffold; Thermosensitive hydrogel