title: Gene polymorphisms of adducin GLY460TRP, ACE I/D, AND AGT M235T in pediatric hypertension patients.
abstract: BACKGROUND
  Hypertension is a major global public health problem that affects both pediatric and adult populations. ACE I/D, AGT M235T, and ADD Gly460Trp polymorphisms are thought to be associated with primary hypertension. In the present study, we examined the frequency of these polymorphisms in a pediatric population with secondary hypertension.
  
  
  METHODS
  Included in the study were 58 hypertensive and 58 normotensive pediatric patients. ACE I/D and AGT M235T polymorphisms are determined by conventional PCR; ADD Gly460Trp polymorphism was investigated using PCR amplification of genomic DNA.
  
  
  RESULTS
  There were significant differences between the control group and pediatric hypertensive group in terms of ACE I/D (P<0.05) and AGT M235T (P<0.05) polymorphisms, but there were no differences in ADD Gly460Trp (P>0.05) polymorphism.
  
  
  CONCLUSIONS
  We suggest that RAS gene polymorphisms (ACE-I/D, AGT M235T) are significantly associated with susceptibility to diseases that lead to secondary hypertension.
authors.affiliation: Department of Medical Biochemistry, Dicle University, Diyarbakir, Turkey.
authors.forename: İbrahim
authors.initials: İ
authors.lastname: Kaplan
authors.affiliation: Department of Medical Biochemistry, Cumhuriyet University, Sivas, Turkey.
authors.forename: Enver
authors.initials: E
authors.lastname: Sancaktar
authors.affiliation: Department of Pediatry, Dicle University, Diyarbakır, Turkey.
authors.forename: Aydın
authors.initials: A
authors.lastname: Ece
authors.affiliation: Department of Pediatry, Dicle University, Diyarbakır, Turkey.
authors.forename: Velat
authors.initials: V
authors.lastname: Şen
authors.affiliation: Central Biochemistry Laboratory, Memorial Hospital Şişli, İstanbul, Turkey.
authors.forename: Nilgün
authors.initials: N
authors.lastname: Tekkeşin
authors.affiliation: Department of Medical Biochemistry, Dicle University, Diyarbakır, Turkey.
authors.forename: Mustafa Kemal
authors.initials: MK
authors.lastname: Basarali
authors.affiliation: Department of Pediatry, Dicle University, Diyarbakır, Turkey.
authors.forename: Selvi
authors.initials: S
authors.lastname: Kelekci
authors.affiliation: Department of Medical Biochemistry, Dicle University, Diyarbakır, Turkey.
authors.forename: Osman
authors.initials: O
authors.lastname: Evliyaoglu
mesh_terms: D000483:Alleles; D000808:Angiotensinogen / Q000235:genetics*; D002148:Calmodulin-Binding Proteins / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D051436:Renal Insufficiency, Chronic / Q000235:genetics; D000099291:Adducins
chemical_list: D000808:Angiotensinogen; D002148:Calmodulin-Binding Proteins; D007703:Peptidyl-Dipeptidase A; D000099291:Adducins; C488034:ACE protein, human