title: Analgesic, anti-inflammatory, and chondroprotective activities of Cryptolepis buchanani extract: in vitro and in vivo studies.
abstract: Cryptolepis buchanani Roem. & Schult. is widely used in folk medicine in Southeast Asia for treating muscle tension and arthritis. This study aimed to investigate an analgesic activity of the methanol extract of C. buchanani (CBE) in acetic acid-induced writhing response in mice, and to examine its anti-inflammatory activity in ethyl phenylpropiolate- (EPP-) induced ear edema and carrageenan-induced paw edema in rats. Its effects on cartilage degradation induced by interleukin-1β (IL-1β) in porcine cartilage explant culture were also determined. This study demonstrated that CBE significantly reduced acetic acid-induced writhing response. It also inhibited edema formation in both EPP-induced ear edema and carrageenan-induced paw edema models. In cartilage explant culture, CBE significantly reduced the sulfated glycosaminoglycan and hyaluronan released into culture media while it reserved the uronic acid and collagen within the cartilage tissues. It also suppressed the matrix metalloproteinase-2 activity with no effect on cell viability. In conclusion, CBE shows analgesic, anti-inflammatory, and chondroprotective effects in this preliminary study. Therefore, CBE may be useful as an alternative treatment for osteoarthritis.
authors.affiliation: Department of Pharmacology and Center of Excellence for Innovation in Chemistry, Faculty of Medicine, Chiang Mai University, Indhawaroros Road, Chiang Mai 50200, Thailand.
authors.forename: Nutthiya
authors.initials: N
authors.lastname: Hanprasertpong
authors.affiliation: Department of Pharmacology and Center of Excellence for Innovation in Chemistry, Faculty of Medicine, Chiang Mai University, Indhawaroros Road, Chiang Mai 50200, Thailand.
authors.forename: Supanimit
authors.initials: S
authors.lastname: Teekachunhatean
authors.affiliation: Department of Medical Technology, Faculty of Associated Medicine, Chiang Mai University, Indhawaroros Road, Chiang Mai 50200, Thailand.
authors.forename: Rujirek
authors.initials: R
authors.lastname: Chaiwongsa
authors.affiliation: Thailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry and Center of Excellence for Innovation in Chemistry, Faculty of Medicine, Chiang Mai University, Indhawaroros Road, Chiang Mai 50200, Thailand.
authors.forename: Siriwan
authors.initials: S
authors.lastname: Ongchai
authors.affiliation: Department of Pharmacology and Center of Excellence for Innovation in Chemistry, Faculty of Medicine, Chiang Mai University, Indhawaroros Road, Chiang Mai 50200, Thailand.
authors.forename: Puongtip
authors.initials: P
authors.lastname: Kunanusorn
authors.affiliation: Department of Pharmacology and Center of Excellence for Innovation in Chemistry, Faculty of Medicine, Chiang Mai University, Indhawaroros Road, Chiang Mai 50200, Thailand.
authors.forename: Chaichan
authors.initials: C
authors.lastname: Sangdee
authors.affiliation: Department of Pharmacology and Center of Excellence for Innovation in Chemistry, Faculty of Medicine, Chiang Mai University, Indhawaroros Road, Chiang Mai 50200, Thailand.
authors.forename: Ampai
authors.initials: A
authors.lastname: Panthong
authors.affiliation: Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand.
authors.forename: Samreang
authors.initials: S
authors.lastname: Bunteang
authors.affiliation: The Forest Herbarium National Park, Wildlife and Plant Conservation Department, Ministry of Natural Resources and Environment, Pholyothin Road, Bangkok 10900, Thailand.
authors.forename: Narong
authors.initials: N
authors.lastname: Nathasaen
authors.affiliation: Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand.
authors.forename: Vichai
authors.initials: V
authors.lastname: Reutrakul
mesh_terms: D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects*; D031106:Cryptolepis / Q000737:chemistry*; D019610:Cytoprotection / Q000187:drug effects / Q000502:physiology; D007249:Inflammation / Q000175:diagnosis / Q000188:drug therapy*; D008297:Male; D051379:Mice; D010146:Pain / Q000175:diagnosis / Q000188:drug therapy*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts