title: The phosphatase mechanism of bifunctional kinase/phosphatase AceK.
abstract: We have revealed that bifunctional AceK kinase/phosphatase utilizes a stepwise addition-elimination mechanism in its dephosphorylation reaction. This work explains how AceK enables opposite kinase and phosphatase activities with Asp477 and a single Mg(2+) ion.
authors.affiliation: College of Chemistry, Beijing Normal University, Beijing, 100875, China. jimin_z@bnu.edu.cn hongwei.tan@bnu.edu.cn.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.forename: Qingya
authors.initials: Q
authors.lastname: Shen
authors.forename: Guangju
authors.initials: G
authors.lastname: Chen
authors.forename: Jimin
authors.initials: J
authors.lastname: Zheng
authors.forename: Hongwei
authors.initials: H
authors.lastname: Tan
authors.forename: Zongchao
authors.initials: Z
authors.lastname: Jia
mesh_terms: D001224:Aspartic Acid / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D007521:Isocitrate Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D008274:Magnesium / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D064251:Multifunctional Enzymes / Q000737:chemistry / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000378:metabolism*; D011789:Quantum Theory
chemical_list: D029968:Escherichia coli Proteins; D064251:Multifunctional Enzymes; D001224:Aspartic Acid; C548023:AceK protein, E coli; D007521:Isocitrate Dehydrogenase; D017346:Protein Serine-Threonine Kinases; D010749:Phosphoprotein Phosphatases; D008274:Magnesium