title: [Identification of pyrrosiae folium and its adulterants based on psbA-trnH sequence].
abstract: In this study, the psbA-trnH sequence as DNA barcode was used to evaluate the accuracy and stability for identification pteridophyte medicinal material Pyrrosiae Foliumas from adulterants. Genomic DNA from 106 samples were extracted successfully. The Kimura 2-Parameter (K2P) distances and ML tree were calculated using software MEGA 6.0. The intra-specific genetic distances of 3 original plants were lower than inter-specific genetic distances of adulterants. The ML tree indicated that Pyrrosiae Folium can be distinguished from its adulterants obviously. Therefore, the psbA-trnH sequence as a barcode of the pteridophyte, can accurately and stably distinguish Pyrrosiae Folium from its adulterants.
authors.forename: Ya-Qin
authors.initials: YQ
authors.lastname: Zhang
authors.forename: Yue
authors.initials: Y
authors.lastname: Shi
authors.forename: Ming
authors.initials: M
authors.lastname: Song
authors.forename: Yun-Han
authors.initials: YH
authors.lastname: Lin
authors.forename: Xiao-Xi
authors.initials: XX
authors.lastname: Ma
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Li
authors.initials: L
authors.lastname: Xiang
authors.forename: Xi
authors.initials: X
authors.lastname: Liu
mesh_terms: D001483:Base Sequence; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D004340:Drug Contamination / Q000517:prevention & control; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000145:classification*; D029624:Ferns / Q000145:classification* / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D011786:Quality Control
chemical_list: D021903:DNA, Ribosomal Spacer; D004365:Drugs, Chinese Herbal; D010940:Plant Proteins