title: Silver-zeolite combined to polyphenol-rich extracts of Ascophyllum nodosum: potential active role in prevention of periodontal diseases.
abstract: The purpose of this study was to evaluate various biological effects of silver-zeolite and a polyphenol-rich extract of A. nodosum (ASCOP) to prevent and/or treat biofilm-related oral diseases. Porphyromonas gingivalis and Streptococcus gordonii contribute to the biofilm formation associated with chronic periodontitis. In this study, we evaluated in vitro antibacterial and anti-biofilm effects of silver-zeolite (Ag-zeolite) combined to ASCOP on P. gingivalis and S. gordonii growth and biofilm formation capacity. We also studied the anti-inflammatory and antioxidant capacities of ASCOP in cell culture models. While Ag-zeolite combined with ASCOP was ineffective against the growth of S. gordonii, it showed a strong bactericidal effect on P. gingivalis growth. Ag-zeolite combined with ASCOP was able to completely inhibit S. gordonii monospecies biofilm formation as well as to reduce the formation of a bi-species S. gordonii/P. gingivalis biofilm. ASCOP alone was ineffective towards the growth and/or biofilm formation of S. gordonii and P. gingivalis while it significantly reduced the secretion of inflammatory cytokines (TNFα and IL-6) by LPS-stimulated human like-macrophages. It also exhibited antioxidant properties and decreased LPS induced lipid peroxidation in gingival epithelial cells. These findings support promising use of these products in future preventive or therapeutic strategies against periodontal diseases.
authors.affiliation: Equipe de Microbiologie, EA 1254, Université Rennes 1, UEB, Rennes, France.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Tamanai-Shacoori
authors.affiliation: Groupe de Recherche en Ecologie Buccale, Faculté de médecine dentaire, Université Laval, Québec City, Québec, Canada.
authors.forename: Fatiha
authors.initials: F
authors.lastname: Chandad
authors.affiliation: Laboratoire Mouvement, Sport, Santé, EA 1274, Université Rennes 1, Université Rennes 2, UEB, Rennes, France.
authors.forename: Amélie
authors.initials: A
authors.lastname: Rébillard
authors.affiliation: Laboratoire Mouvement, Sport, Santé, EA 1274, Université Rennes 1, Université Rennes 2, UEB, Rennes, France.
authors.forename: Josiane
authors.initials: J
authors.lastname: Cillard
authors.affiliation: Equipe de Microbiologie, EA 1254, Université Rennes 1, UEB, Rennes, France; Centre hospitalo-universitaire, Rennes, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Bonnaure-Mallet
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D044646:Ascophyllum / Q000737:chemistry*; D018441:Biofilms / Q000187:drug effects; D045744:Cell Line, Tumor; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D010510:Periodontal Diseases / Q000382:microbiology / Q000517:prevention & control; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology*; D016966:Porphyromonas gingivalis / Q000187:drug effects; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D054773:Streptococcus gordonii / Q000187:drug effects; D017641:Zeolites / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016207:Cytokines; D010936:Plant Extracts; D059808:Polyphenols; D017641:Zeolites; D012834:Silver