title: Examination of the role of galectins in intestinal inflammation.
abstract: The intestine, which provides the first line of defense against over trillion of enteric microorganisms, suffers from broad range of inflammatory conditions caused by infectious, autoimmune, allergic, neurological, and ischemic mechanisms. Recent data have suggested dual roles (protective versus deleterious) for galectins in the pathogenesis of some intestinal inflammations, highlighting the importance of this area of research. A potential problem with the research of intestinal inflammation may be the requirement of some unique techniques. Therefore, we herein describe how to induce intestinal inflammation and how to isolate lymphocyte, myeloid cell, follicular cell, and epithelial cell populations separately from the intestine for the study of intestinal inflammations.
authors.affiliation: Molecular Pathology Unit, Center for the Study of Inflammatory Bowel Disease, Massachusetts General Hospital, Boston, MA, USA.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nishida
authors.forename: Cindy W
authors.initials: CW
authors.lastname: Lau
authors.forename: Atsushi
authors.initials: A
authors.lastname: Mizoguchi
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology; D002469:Cell Separation; D003092:Colitis / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D037161:Galectins / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D007410:Intestinal Diseases / Q000276:immunology / Q000378:metabolism* / Q000473:pathology*; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008264:Macrophages / Q000166:cytology; D008297:Male; D051379:Mice; D013601:T-Lymphocytes / Q000166:cytology
chemical_list: D037161:Galectins; D017493:Leukocyte Common Antigens