title: The role of a single nucleotide polymorphism of the matrix metalloproteinase-1 gene promoter region in invasion and prognosis of meningiomas.
abstract: OBJECTIVE
  The matrix metalloproteinase (MMP) enzyme family has been shown to be active in tumorigenesis and tumor progression. In this study, we analyzed the prevalence of a guanine insertion in the MMP-1 gene promoter region in meningiomas and its effect on invasion and prognosis.
  
  
  METHODS
  The study was performed with 33 meningioma patients. We also included 33 healthy patients in the study as a control group. The promoter area was amplified by polymerase chain reaction (PCR) following DNA isolation. The polymorphism was detected by restriction fragment length polymorphism (RFLP).
  
  
  RESULTS
  According to the WHO classification of meningiomas, 87.9% of the affected patients were grade 1, and 12.1% were grade 2. In total, 72.7% of the meningioma patients (n=24) had at least one copy of the insertion (2G/1G or 2G/2G genotypes) and 27.3% (n=9) did not (1G/1G). There was no significant difference between the meningioma and control groups according to genotype distribution.
  
  
  CONCLUSIONS
  In this study, the polymorphism in the matrix metalloproteinase-1 gene promoter region did not have an effect on the initiation, growth and progression of meningioma.
authors.affiliation: Ministry of Health, Dr. Lutfi Kirdar Kartal Training and Research Hospital, Neurosurgery Clinic, Istanbul, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Efendioglu
authors.forename: Recep
authors.initials: R
authors.lastname: Basaran
authors.forename: Omer Faruk
authors.initials: OF
authors.lastname: Bayrak
authors.forename: Nejat
authors.initials: N
authors.lastname: Isik
authors.forename: Tuncay
authors.initials: T
authors.lastname: Kaner
authors.forename: Fikrettin
authors.initials: F
authors.lastname: Sahin
authors.forename: Bulent
authors.initials: B
authors.lastname: Guclu
authors.forename: Ilhan
authors.initials: I
authors.lastname: Elmaci
mesh_terms: D000293:Adolescent; D000328:Adult; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002648:Child; D004247:DNA / Q000032:analysis; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000235:genetics*; D008577:Meningeal Neoplasms / Q000235:genetics* / Q000473:pathology; D008579:Meningioma / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D011401:Promoter Regions, Genetic
chemical_list: D004247:DNA; D020781:Matrix Metalloproteinase 1