title: Folding pathway of a multidomain protein depends on its topology of domain connectivity.
abstract: How do the folding mechanisms of multidomain proteins depend on protein topology? We addressed this question by developing an Ising-like structure-based model and applying it for the analysis of free-energy landscapes and folding kinetics of an example protein, Escherichia coli dihydrofolate reductase (DHFR). DHFR has two domains, one comprising discontinuous N- and C-terminal parts and the other comprising a continuous middle part of the chain. The simulated folding pathway of DHFR is a sequential process during which the continuous domain folds first, followed by the discontinuous domain, thereby avoiding the rapid decrease in conformation entropy caused by the association of the N- and C-terminal parts during the early phase of folding. Our simulated results consistently explain the observed experimental data on folding kinetics and predict an off-pathway structural fluctuation at equilibrium. For a circular permutant for which the topological complexity of wild-type DHFR is resolved, the balance between energy and entropy is modulated, resulting in the coexistence of the two folding pathways. This coexistence of pathways should account for the experimentally observed complex folding behavior of the circular permutant.
authors.affiliation: Department of Computational Science and Engineering, Nagoya University, Nagoya 464-8603, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Inanami
authors.affiliation: Department of Computational Science and Engineering, Nagoya University, Nagoya 464-8603, Japan.
authors.forename: Tomoki P
authors.initials: TP
authors.lastname: Terada
authors.affiliation: Department of Computational Science and Engineering, Nagoya University, Nagoya 464-8603, Japan sasai@cse.nagoya-u.ac.jp.
authors.forename: Masaki
authors.initials: M
authors.lastname: Sasai
mesh_terms: D019943:Amino Acid Substitution; D007700:Kinetics; D008956:Models, Chemical; D020125:Mutation, Missense; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D013762:Tetrahydrofolate Dehydrogenase
keywords: eWSME model; folding intermediates; internal friction