title: Production of optically pure d-lactate from glycerol by engineered Klebsiella pneumoniae strain.
abstract: In this study, glycerol was used to produce optically pure d-lactate by engineered Klebsiella pneumoniae strain. In the recombinant strain, d-lactate dehydrogenase LdhA was overexpressed, and two genes, dhaT and yqhD for biosynthesis of main byproduct 1,3-propanediol, were knocked out. To further improve d-lactate production, the culture condition was optimized and the results demonstrated that aeration rate played an important role in d-lactate production. In microaerobic fed-batch fermentation, the engineered strain accumulated 142.1g/L optically pure d-lactate with a yield of 0.82g/g glycerol, which represented the highest d-lactate production from glycerol so far. This study showed that K. pneumoniae strain has high efficiency to convert glycerol into d-lactate and high potentiality in utilization of crude glycerol from biodiesel industry.
authors.affiliation: CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Xinjun
authors.initials: X
authors.lastname: Feng
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Yamei
authors.initials: Y
authors.lastname: Ding
authors.affiliation: CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
authors.forename: Mo
authors.initials: M
authors.lastname: Xian
authors.affiliation: CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.affiliation: CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
authors.forename: Rubing
authors.initials: R
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China. Electronic address: zhaoguang@qibebt.ac.cn.
authors.forename: Guang
authors.initials: G
authors.lastname: Zhao
mesh_terms: D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D019344:Lactic Acid / Q000096:biosynthesis*; D060847:Metabolic Engineering; D017672:Nitrogen Compounds; D010084:Oxidation-Reduction
chemical_list: D017672:Nitrogen Compounds; D019344:Lactic Acid; D005990:Glycerol
keywords: Bioconversion; Engineered Klebsiella pneumoniae strain; Glycerol; d-Lactate