title: Proteomic study of pilocytic astrocytoma pediatric brain tumor intracystic fluid.
abstract: Liquid chromatography in coupling with high-resolution ESI-LTQ-Orbitrap mass spectrometry was applied for a proteomic study of pediatric pilocytic astrocytoma brain tumor intracystic fluid by an integrated top-down/bottom-up platform. Both of the proteomic strategies resulted complementary and support each other in contributing to a wide characterization of the protein and peptide content of the tumor fluid. Top-down approach allowed to identify several proteins and peptides involved in different biological activities together with the characterization of interesting proteoforms such as fibrinopeptide A and its truncated form, fibrinopeptide B, complement C3f fragments, β-thymosin peptides, ubiquitin, several apolipoproteins belonging to A and C families, apolipoprotein J and D, and cystatin C. Of particular interest resulted the identification of a N-terminal truncated cystatin C proteoform, likely involved in immune response mechanism modulations and the identification of oxidized and glycosylated apolipoproteins including disulfide bridge dimeric forms. The bottom-up approach confirmed some of the experimental data findings together with adding the characterization of high-molecular-mass proteins in the samples. These data could contribute to elucidate the molecular mechanisms involved in onset and progression of the disease and cyst development.
authors.affiliation: Istituto di Biochimica e Biochimica Clinica, Facoltà di Medicina, Università Cattolica del Sacro Cuore , Rome 00168, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Inserra
authors.forename: Federica
authors.initials: F
authors.lastname: Iavarone
authors.forename: Claudia
authors.initials: C
authors.lastname: Martelli
authors.forename: Luca
authors.initials: L
authors.lastname: D'Angelo
authors.forename: Daniela
authors.initials: D
authors.lastname: Delfino
authors.forename: Diana Valeria
authors.initials: DV
authors.lastname: Rossetti
authors.forename: Gianpiero
authors.initials: G
authors.lastname: Tamburrini
authors.forename: Luca
authors.initials: L
authors.lastname: Massimi
authors.forename: Massimo
authors.initials: M
authors.lastname: Caldarelli
authors.forename: Concezio
authors.initials: C
authors.lastname: Di Rocco
authors.forename: Irene
authors.initials: I
authors.lastname: Messana
authors.forename: Massimo
authors.initials: M
authors.lastname: Castagnola
authors.forename: Claudia
authors.initials: C
authors.lastname: Desiderio
mesh_terms: D001254:Astrocytoma / Q000378:metabolism*; D002648:Child; D002851:Chromatography, High Pressure Liquid; D020420:Cyst Fluid / Q000378:metabolism*; D055316:Cystatin C / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D040901:Proteomics / Q000379:methods*
chemical_list: D055316:Cystatin C
keywords: brain tumors; intracystic fluid; mass spectrometry; proteomics; pylocitic astrocytoma