title: Immobilization and stabilization of lipase (CaLB) through hierarchical interfacial assembly.
abstract: Nanostructure-enabled hierarchical assembly holds promise for efficient biocatalyst immobilization for improved stability in bioprocessing. In this work we demonstrate the use of a hierarchical assembly immobilization strategy to enhance the physicochemical properties and stability of lipase B from Candida antarctica (CaLB). CaLB was complexed with iron oxide nanoparticles followed by interfacial assembly at the surface of an oil-in-water emulsion. Subsequent ring opening polymerization of the oil provided cross-linked microparticles that displayed an increase in catalytic efficiency when compared to the native enzyme and Novozym 435. The hierarchical immobilized enzyme assembly showed no leakage from the support in 50% acetonitrile and could be magnetically recovered across five cycles. Immobilized lipase exhibited enhanced thermal and pH stability, providing 72% activity retention after 24 h at 50 °C (pH 7.0) and 62% activity retention after 24 h at pH 3.0 (30 °C); conditions resulting in complete deactivation of the native lipase.
authors.affiliation: Department of Food Science, University of Massachusetts - Amherst , 102 Holdsworth Way, Amherst, Massachusetts 01003, United States.
authors.forename: Joey N
authors.initials: JN
authors.lastname: Talbert
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Wang
authors.forename: Bradley
authors.initials: B
authors.lastname: Duncan
authors.forename: Youngdo
authors.initials: Y
authors.lastname: Jeong
authors.forename: Stephanie M
authors.initials: SM
authors.lastname: Andler
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Rotello
authors.forename: Julie M
authors.initials: JM
authors.lastname: Goddard
mesh_terms: D004795:Enzyme Stability / Q000502:physiology; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry* / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D005290:Ferric Compounds; D005656:Fungal Proteins; C000499:ferric oxide; C093667:Novozyme 435; D008049:Lipase; C431349:lipase B, Candida antarctica