title: Transplantation of stem cells from human exfoliated deciduous teeth for bone regeneration in the dog mandibular defect.
abstract: OBJECTIVE
  To investigate the effect of stem cells from human exfoliated deciduous teeth (SHED) transplanted for bone regeneration in the dog mandibular defect.
  
  
  METHODS
  In this prospective comparative study, SHEDs had been isolated 5 years ago from human exfoliated deciduous teeth. The undifferentiated stem cells were seeded into mandibular bone through-and-through defects of 4 dogs. Similar defects in control group were filled with cell-free collagen scaffold. After 12 wk, biopsies were taken and morphometric analysis was performed. The percentage of new bone formation and foreign body reaction were measured in each case. The data were subject to statistical analysis using the Mann-Whitney U and Kruskalwalis statistical tests. Differences at P < 0.05 was considered as significant level.
  
  
  RESULTS
  There were no significant differences between control and SHED-seeded groups in connective tissue (P = 0.248), woven bone (P = 0.248) and compact bone (P = 0.082). There were not any side effects in transplanted SHED group such as teratoma or malignancy and abnormalities in this period.
  
  
  CONCLUSIONS
  SHEDs which had been isolated and characterized 5 years ago and stored with cryopreservation banking were capable of proliferation and osteogenesis after 5 years, and no immune response was observed after three months of seeded SHEDs.
authors.affiliation: Ali Behnia, Member of Students' Research Center, Isfahan University of Medical Sciences, 81745-319 Isfahan, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Behnia
authors.affiliation: Ali Behnia, Member of Students' Research Center, Isfahan University of Medical Sciences, 81745-319 Isfahan, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Haghighat
authors.affiliation: Ali Behnia, Member of Students' Research Center, Isfahan University of Medical Sciences, 81745-319 Isfahan, Iran.
authors.forename: Ardeshir
authors.initials: A
authors.lastname: Talebi
authors.affiliation: Ali Behnia, Member of Students' Research Center, Isfahan University of Medical Sciences, 81745-319 Isfahan, Iran.
authors.forename: Nosrat
authors.initials: N
authors.lastname: Nourbakhsh
authors.affiliation: Ali Behnia, Member of Students' Research Center, Isfahan University of Medical Sciences, 81745-319 Isfahan, Iran.
authors.forename: Fariba
authors.initials: F
authors.lastname: Heidari
keywords: Bone regeneration; Isolation; Stem cells from human exfoliated deciduous teeth