title: Multiplex isotope dimethyl labeling of substrate peptides for high throughput kinase activity assay via quantitative MALDI MS.
abstract: A simple, cost-effective and high throughput method was developed for multiplexed kinase activity assay based on the multiplex isotope labeling of designed substrate peptides. This strategy was successfully applied to monitor the time-dependent consumption of substrates and generation of products in the single and multiple substrate systems.
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China. mingliang@dicp.ac.cn hanfazou@dicp.ac.cn.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Deng
authors.forename: Mingliang
authors.initials: M
authors.lastname: Ye
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Bian
authors.forename: Zheyi
authors.initials: Z
authors.lastname: Liu
authors.forename: Fangjie
authors.initials: F
authors.lastname: Liu
authors.forename: Chunli
authors.initials: C
authors.lastname: Wang
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Qin
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
mesh_terms: D004789:Enzyme Activation; D057166:High-Throughput Screening Assays; D007553:Isotope Labeling; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013379:Substrate Specificity
chemical_list: D010455:Peptides; D011494:Protein Kinases