title: Effects of different concentrations of Pseudomonas aeruginosa on boar sperm quality.
abstract: Bacteriospermia in boar ejaculates is a frequent finding that compromises the sperm quality and, consequently, causes economic losses in swine industry. The present study sought to evaluate the effect of different concentrations of Pseudomonas aeruginosa on boar sperm quality over a storing period of 11 days at 15-17 ° C. Ten commercial seminal doses coming from post-pubertal and healthy boars were artificially inoculated with different infective concentrations of P. aeruginosa, ranging from 2 × 10(8) to 2 × 10(4)cfu/mL. Negative controls were non-inoculated doses. Sperm quality, assessed as sperm motility (CASA), sperm viability, acrosome integrity and pH, as well as the bacterial growth, were checked after 0, 1, 2, 4, 7, 9 and 11 days of storage at 15-17 ° C. Results obtained showed significant decreases in the percentages of total and progressive sperm motility, sperm viability and acrosome integrity in the greatest infective concentrations (2 × 10(7) and 2 × 10(8)cfu/mL), when compared to the negative control. In contrast, there was no effect on seminal pH throughout the experiment. Results indicate the presence of P. aeruginosa in boar semen, apart from being a potential source for the spread of infectious diseases and harmful impact on sows, negatively affects the longevity and fertilizing ability of boar sperm when present in high concentrations. Thus, P. aeruginosa causes deleterious effects on boar sperm quality during liquid storage at 15-17 ° C, thus strict hygienic measures must be implemented in boar studs to minimize bacterial concentration of semen doses.
authors.affiliation: Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, 17071 Girona, Catalonia, Spain. Electronic address: liliana.sepulveda@udg.edu.
authors.forename: Lilian
authors.initials: L
authors.lastname: Sepúlveda
authors.affiliation: Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, 17071 Girona, Catalonia, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Bussalleu
authors.affiliation: Unit of Animal Reproduction, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, 08193 Bellaterra (Barcelona), Catalonia, Spain.
authors.forename: Marc
authors.initials: M
authors.lastname: Yeste
authors.affiliation: Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, 17071 Girona, Catalonia, Spain.
authors.forename: Sergi
authors.initials: S
authors.lastname: Bonet
mesh_terms: D000818:Animals; D008297:Male; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D055101:Semen Analysis / Q000662:veterinary*; D012662:Semen Preservation; D013081:Sperm Motility; D013094:Spermatozoa / Q000382:microbiology*; D013552:Swine / Q000502:physiology*; D013997:Time Factors
keywords: Bacteria; Boar spermatozoa; Pseudomonas aeruginosa; Sperm quality