title: Faecal matrix metalloprotease-9 is a more sensitive marker for diagnosing pouchitis than faecal calprotectin: results from a pilot study.
abstract: BACKGROUND
  Potential non-invasive markers of pouchitis would have a great deal of significance within clinical practice.
  
  
  OBJECTIVE
  This study is aimed at assessing the diagnostic accuracy of fecal calprotectin and matrix metalloprotease-9 as potential markers in patients both with and without pouchitis.
  
  
  METHODS
  Stool and blood samples were collected from 33 ileal pouch-anal anastomosis patients before a follow-up pouchoscopy. Biopsy samples were taken for histological purposes. The presence of cuffitis and stenosis was evaluated with an endoscopy. Calprotectin and matrix metalloprotease-9 were quantified with an enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Pouchitis was detected in 30.3% of the patients. The levels of fecal calprotectin and matrix metalloprotease-9 increased significantly in patients with pouchitis. The sensitivity and specificity of matrix metalloprotease-9 was higher than that of fecal calprotectin. Only matrix metalloprotease-9 correlated significantly with the severity of pouchitis.
  
  
  CONCLUSIONS
  Fecal matrix metalloprotease-9 has a high specificity in the diagnosis of pouchitis.
authors.affiliation: First Department of Medicine, University of Szeged H-6720, Korányi fasor 8, Szeged, Hungary.
authors.forename: Klaudia
authors.initials: K
authors.lastname: Farkas
authors.forename: Anita
authors.initials: A
authors.lastname: Bálint
authors.forename: Renáta
authors.initials: R
authors.lastname: Bor
authors.forename: Imre
authors.initials: I
authors.lastname: Földesi
authors.forename: Mónika
authors.initials: M
authors.lastname: Szűcs
authors.forename: Ferenc
authors.initials: F
authors.lastname: Nagy
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Szepes
authors.forename: Anita
authors.initials: A
authors.lastname: Annaházi
authors.forename: Richárd
authors.initials: R
authors.lastname: Róka
authors.forename: Tamás
authors.initials: T
authors.lastname: Molnár
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D004796:Clinical Enzyme Tests; D003093:Colitis, Ulcerative / Q000601:surgery; D016099:Endoscopy, Gastrointestinal; D005243:Feces / Q000737:chemistry* / Q000201:enzymology; D005260:Female; D006801:Humans; D039841:Leukocyte L1 Antigen Complex / Q000032:analysis; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000032:analysis*; D008875:Middle Aged; D010865:Pilot Projects; D019449:Pouchitis / Q000175:diagnosis*; D016737:Proctocolectomy, Restorative; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D015415:Biomarkers; D039841:Leukocyte L1 Antigen Complex; D020780:Matrix Metalloproteinase 9
keywords: fecal calprotectin; ileal pouch-anal anastomosis; matrix metalloprotease-9; pouchitis; ulcerative colitis