title: Doxycycline enhances survival and self-renewal of human pluripotent stem cells.
abstract: We here report that doxycycline, an antibacterial agent, exerts dramatic effects on human embryonic stem and induced pluripotent stem cells (hESC/iPSCs) survival and self-renewal. The survival-promoting effect was also manifest in cultures of neural stem cells (NSCs) derived from hESC/iPSCs. These doxycycline effects are not associated with its antibacterial action, but mediated by direct activation of a PI3K-AKT intracellular signal. These findings indicate doxycycline as a useful supplement for stem cell cultures, facilitating their growth and maintenance.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Seoul 133-791, Korea; Hanyang Biomedical Research Institute, Hanyang University, Seoul 133-791, Korea.
authors.forename: Mi-Yoon
authors.initials: MY
authors.lastname: Chang
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Seoul 133-791, Korea; Hanyang Biomedical Research Institute, Hanyang University, Seoul 133-791, Korea; Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 133-791, Korea.
authors.forename: Yong-Hee
authors.initials: YH
authors.lastname: Rhee
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Seoul 133-791, Korea; Hanyang Biomedical Research Institute, Hanyang University, Seoul 133-791, Korea.
authors.forename: Sang-Hoon
authors.initials: SH
authors.lastname: Yi
authors.affiliation: Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 133-791, Korea.
authors.forename: Su-Jae
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 133-791, Korea.
authors.forename: Rae-Kwon
authors.initials: RK
authors.lastname: Kim
authors.affiliation: Hanyang Biomedical Research Institute, Hanyang University, Seoul 133-791, Korea; Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 133-791, Korea.
authors.forename: Hyongbum
authors.initials: H
authors.lastname: Kim
authors.affiliation: Hanyang Biomedical Research Institute, Hanyang University, Seoul 133-791, Korea; Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 133-791, Korea.
authors.forename: Chang-Hwan
authors.initials: CH
authors.lastname: Park
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Seoul 133-791, Korea; Hanyang Biomedical Research Institute, Hanyang University, Seoul 133-791, Korea; Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 133-791, Korea. Electronic address: leesh@hanyang.ac.kr.
authors.forename: Sang-Hun
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004318:Doxycycline / Q000494:pharmacology*; D053595:Embryonic Stem Cells / Q000166:cytology; D006801:Humans; D007621:Karyotyping; D058953:Neural Stem Cells / Q000166:cytology; D009474:Neurons / Q000166:cytology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D004318:Doxycycline