title: Vitamin C mitigates oxidative stress and tumor necrosis factor-alpha in severe community-acquired pneumonia and LPS-induced macrophages.
abstract: Oxidative stress is an important part of host innate immune response to foreign pathogens. However, the impact of vitamin C on oxidative stress and inflammation remains unclear in community-acquired pneumonia (CAP). We aimed to determine the effect of vitamin C on oxidative stress and inflammation. CAP patients were enrolled. Reactive oxygen species (ROS), DNA damage, superoxide dismutases (SOD) activity, tumor necrosis factor-alpha (TNF-α), and IL-6 were analyzed in CAP patients and LPS-stimulated macrophages cells. MH-S cells were transfected with RFP-LC3 plasmids. Autophagy was measured in LPS-stimulated macrophages cells. Severe CAP patients showed significantly increased ROS, DNA damage, TNF-α, and IL-6. SOD was significantly decreased in severe CAP. Vitamin C significantly decreased ROS, DNA damage, TNF-α, and IL-6. Vitamin C inhibited LPS-induced ROS, DNA damage, TNF-α, IL-6, and p38 in macrophages cells. Vitamin C inhibited autophagy in LPS-induced macrophages cells. These findings indicated that severe CAP exhibited significantly increased oxidative stress, DNA damage, and proinflammatory mediator. Vitamin C mitigated oxidative stress and proinflammatory mediator suggesting a possible mechanism for vitamin C in severe CAP.
authors.affiliation: Respiratory Section, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Hygiene Section, Luzhou Medical College, Luzhou, Sichuan 646000, China.
authors.forename: Guangyan
authors.initials: G
authors.lastname: Luo
authors.affiliation: Respiratory Section, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Respiratory Section, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Respiratory Section, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China.
authors.forename: Xiaoqiong
authors.initials: X
authors.lastname: Yang
authors.affiliation: Respiratory Section, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Wang
authors.affiliation: Respiratory Section, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China ; State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, School of Medicine, Shenzhen University, Nanhai Avenue 3688, Shenzhen, Guangdong 518060, China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Li
authors.affiliation: State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, School of Medicine, Shenzhen University, Nanhai Avenue 3688, Shenzhen, Guangdong 518060, China.
authors.forename: Zhiguang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangdong 510120, China.
authors.forename: Nanshan
authors.initials: N
authors.lastname: Zhong
mesh_terms: D000368:Aged; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D015153:Blotting, Western; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D015850:Interleukin-6; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D001205:Ascorbic Acid