title: Does the upstream region possessing MULE-like sequence in rice upregulate PsbS1 gene expression?
abstract: The genomic nucleotide sequences of japonica rice (Sasanishiki and Nipponbare) contained about 2.7-kb unique region at the point of 0.4-kb upstream of the OsPsbS1 gene. In this study, we found that japonica rice with a few exceptions possessing such DNA sequences [denoted to OsMULE-japonica specific sequence (JSS)] is distinct by the presence of Mutator-like-element (MULE). Such sequence was absent in most of indica cultivars and Oryza glaberrima. In OsMULE-JSS1, we noted the presence of possible target site duplication (TSD; CTTTTCCAG) and about 80-bp terminal inverted repeat (TIR) near TSD. We also found the enhancement ofOsPsbS1 mRNA accumulation by intensified light, which was not associated with the DNA methylation status in OsMULE/JSS. In addition, O. rufipogon, possible ancestor of modern rice cultivars was found to compose PsbS gene of either japonica (minor) or indica (major) type. Transient gene expression assay showed that the japonica type promoter elevated a reporter gene activity than indica type.
authors.affiliation: Institute for Environmental Science and Technology, Saitama University, Saitama city, Saitama, Japan; Graduate School of Science and Engineering, Saitama University, Saitama City, Saitama, Japan.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Nuruzzaman
authors.affiliation: Japanese Science and Technology Agency, PRESTO, Saitama, Japan; Plant Genome Engineering Research Unit, Agrogenomics Research Center, National Institute of Agrobiological Sciences, Ibaraki, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kanno
authors.affiliation: Institute for Environmental Science and Technology, Saitama University, Saitama city, Saitama, Japan.
authors.forename: Rika
authors.initials: R
authors.lastname: Amada
authors.affiliation: Plant Genome Engineering Research Unit, Agrogenomics Research Center, National Institute of Agrobiological Sciences, Ibaraki, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Habu
authors.affiliation: National Agriculture and Food Research Organization, Institute of Floricultural Science, Tsukuba, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kasajima
authors.affiliation: Graduate School of Science and Engineering, Saitama University, Saitama City, Saitama, Japan.
authors.forename: Toshiki
authors.initials: T
authors.lastname: Ishikawa
authors.affiliation: Institute for Environmental Science and Technology, Saitama University, Saitama city, Saitama, Japan; Graduate School of Science and Engineering, Saitama University, Saitama City, Saitama, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Kawai-Yamada
authors.affiliation: Institute for Environmental Science and Technology, Saitama University, Saitama city, Saitama, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Uchimiya
mesh_terms: D001483:Base Sequence; D032461:Chromosomes, Plant; D019175:DNA Methylation; D018628:Gene Dosage; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D023061:Gene Order; D017343:Genes, Plant; D017930:Genes, Reporter; D018745:Genome, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D020079:Terminal Repeat Sequences; D014158:Transcription, Genetic; D015533:Transcriptional Activation