title: Development and validation of an LC-MS/MS method for the toxicokinetic study of deoxynivalenol and its acetylated derivatives in chicken and pig plasma.
abstract: This study aims to develop an LC-MS/MS method allowing the determination of 3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol, deoxynivalenol and its main in vivo metabolite, deepoxy-deoxynivalenol, in broiler chickens and pigs. These species have a high exposure to these toxins, given their mainly cereal based diet. Several sample cleanup strategies were tested and further optimized by means of fractional factorial designs. A simple and straightforward sample preparation method was developed consisting out of a deproteinisation step with acetonitrile, followed by evaporation of the supernatant and reconstitution in water. The method was single laboratory validated according to European guidelines and found to be applicable for the intended purpose, with a linear response up to 200ngml(-1) and limits of quantification of 0.1-2ngml(-1). As a proof of concept, biological samples from a broiler chicken that received either deoxynivalenol, 3- or 15-acetyl-deoxynivalenol were analyzed. Preliminary results indicate nearly complete hydrolysis of 3-acetyl-deoxynivalenol to deoxynivalenol; and to a lesser extent of 15-acetyl-deoxynivalenol to deoxynivalenol. No deepoxy-deoxynivalenol was detected in any of the plasma samples. The method will be applied to study full toxicokinetic properties of deoxynivalenol, 3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol in broiler chickens and pigs.
authors.affiliation: Ghent University, Faculty of Veterinary Medicine, Department of Pharmacology, Toxicology and Biochemistry, Salisburylaan 133, 9820 Merelbeke, Belgium. Electronic address: nathan.broekaert@ugent.be.
authors.forename: N
authors.initials: N
authors.lastname: Broekaert
authors.affiliation: Ghent University, Faculty of Veterinary Medicine, Department of Pharmacology, Toxicology and Biochemistry, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Devreese
authors.affiliation: Ghent University, Faculty of Veterinary Medicine, Department of Pharmacology, Toxicology and Biochemistry, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: T
authors.initials: T
authors.lastname: De Mil
authors.affiliation: Ghent University, Faculty of Veterinary Medicine, Department of Pharmacology, Toxicology and Biochemistry, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: Fraeyman
authors.affiliation: Ghent University, Faculty of Veterinary Medicine, Department of Pharmacology, Toxicology and Biochemistry, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: De Baere
authors.affiliation: Ghent University, Faculty of Pharmaceutical Sciences, Department of Bioanalysis, Laboratory of Food Analysis, Ottergemsesteenweg 460, 9000 Gent, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: De Saeger
authors.affiliation: Ghent University, Faculty of Veterinary Medicine, Department of Pharmacology, Toxicology and Biochemistry, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: De Backer
authors.affiliation: Ghent University, Faculty of Veterinary Medicine, Department of Pharmacology, Toxicology and Biochemistry, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: Croubels
mesh_terms: D000818:Animals; D002645:Chickens / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D010865:Pilot Projects; D012680:Sensitivity and Specificity; D034421:Sus scrofa / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D066007:Toxicokinetics; D014255:Trichothecenes / Q000097:blood* / Q000633:toxicity
chemical_list: D014255:Trichothecenes; C043247:3-acetyldeoxynivalenol; C048238:deepoxy-deoxynivalenol; C046760:15-acetyldeoxynivalenol; C007262:deoxynivalenol
keywords: 15-Acetyl-deoxynivalenol (15ADON); 3-Acetyl-deoxynivalenol (3ADON); Deoxynivalenol (DON); LC–MS/MS; Pig; Poultry