title: Cyclin D1 acts as a barrier to pluripotent reprogramming by promoting neural progenitor fate commitment.
abstract: A short G1 phase is a characteristic feature of the cell cycle structure of pluripotent cells, and is reestablished during Yamanaka factor-mediated pluripotent reprogramming. How cell cycle control is adjusted to meet the requirements of pluripotent cell fate commitment during reprogramming is less well understood. Elevated levels of cyclin D1 were initially found to impair pluripotency maintenance. The current work further identified Cyclin D1 to be capable of transcriptionally upregulating Pax6, which promoted reprogramming cells to commit to a neural progenitor fate rather than a pluripotent cell fate. These findings explain the importance of reestablishment of G1-phase restriction in pluripotent reprogramming.
authors.affiliation: Graduate Institute of Life Science, National Defense Medical Center, Taipei 114, Taiwan; Genomics Research Center, Academic Sinica, Taipei 115, Taiwan.
authors.forename: Chih-Lung
authors.initials: CL
authors.lastname: Chen
authors.affiliation: Genomics Research Center, Academic Sinica, Taipei 115, Taiwan.
authors.forename: Liang-Jie
authors.initials: LJ
authors.lastname: Wang
authors.affiliation: Institute of Biomedical Sciences, Academic Sinica, Taipei 115, Taiwan.
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Yan
authors.affiliation: Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei 112, Taiwan.
authors.forename: Hung-Wei
authors.initials: HW
authors.lastname: Hsu
authors.affiliation: Department of Life Sciences, National Chung-Hsing University, Taichung 402, Taiwan.
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Su
authors.affiliation: Graduate Institute of Life Science, National Defense Medical Center, Taipei 114, Taiwan.
authors.forename: Fang-Pei
authors.initials: FP
authors.lastname: Chang
authors.affiliation: Institute of Biomedical Sciences, Academic Sinica, Taipei 115, Taiwan.
authors.forename: Patrick C H
authors.initials: PC
authors.lastname: Hsieh
authors.affiliation: Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu 300, Taiwan.
authors.forename: Shiaw-Min
authors.initials: SM
authors.lastname: Hwang
authors.affiliation: Graduate Institute of Life Science, National Defense Medical Center, Taipei 114, Taiwan; Genomics Research Center, Academic Sinica, Taipei 115, Taiwan; Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei 112, Taiwan. Electronic address: cnshen@gate.sinica.edu.tw.
authors.forename: Chia-Ning
authors.initials: CN
authors.lastname: Shen
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D019938:Cyclin D1 / Q000096:biosynthesis / Q000378:metabolism*; D005136:Eye Proteins / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology; D016193:G1 Phase; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D051379:Mice; D000071317:Nanog Homeobox Protein; D058953:Neural Stem Cells / Q000166:cytology*; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors / Q000235:genetics; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D015415:Biomarkers; D005136:Eye Proteins; D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse; D000071841:PAX6 Transcription Factor; C000605730:PAX6 protein, human; D051761:Paired Box Transcription Factors; C000605732:Pax6 protein, mouse; D012097:Repressor Proteins; D019938:Cyclin D1
keywords: Cell cycle; Cyclin D; Induced neural stem/progenitor cell; Induced pluripotent stem cell