title: Doping hydroxylated cationic lipid into PEGylated cerasome boosts in vivo siRNA transfection efficacy.
abstract: The therapeutic application of small interfering RNA (siRNA) requires safe nanocarriers for specific and efficient delivery in vivo. Herein, PEGylated cationic cerasomes (PCCs) were fabricated by doping a cationic lipid with a hydroxyl group into nanohybrid cerasomes. Multiple properties of PCCs provide a solution to many of the limitations associated with current platforms for the delivery of siRNA. The polyorganosiloxane surface imparts PCCs with higher morphological stability than conventional liposomes. The PEGylation of the cationic cerasome could protect the cerasome nanoparticles from agglomeration and macrophage capture, reduce protein absorption, and consequently prolong the blood circulating time and enhance the siRNA delivery efficiency. In addition, incorporation of the lipid containing a hydroxyl group further facilitates endosome release. Moreover, PCCs were further used to transport siRNA into the cytosol primarily via endocytosis. When applied to systemic administration, PCCs have demonstrated effective delivery into the liver and preferential uptake by hepatocytes in mice, thereby leading to high siRNA gene-silencing activity. All these results show potential therapeutic applications of PCCs-mediated delivery of siRNA for liver diseases.
authors.affiliation: Department of Biomedical Engineering, College of Engineering, Peking University , Beijing 100871, P.R. China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Li
authors.forename: Shuquan
authors.initials: S
authors.lastname: Zheng
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Liang
authors.forename: Yushen
authors.initials: Y
authors.lastname: Jin
authors.forename: Yidi
authors.initials: Y
authors.lastname: Wu
authors.forename: Huichen
authors.initials: H
authors.lastname: Bai
authors.forename: Renfa
authors.initials: R
authors.lastname: Liu
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Dai
authors.forename: Zicai
authors.initials: Z
authors.lastname: Liang
authors.forename: Tiejun
authors.initials: T
authors.lastname: Shi
mesh_terms: D000818:Animals; D001692:Biological Transport; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D020868:Gene Silencing; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D006900:Hydroxylation; D008081:Liposomes; D008099:Liver / Q000378:metabolism; D051379:Mice; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D004337:Drug Carriers; D008081:Liposomes; D010714:Phosphatidylethanolamines; D034741:RNA, Small Interfering; C038089:1,2-distearoylphosphatidylethanolamine; D011092:Polyethylene Glycols