title: Magnetic nanoparticle DNA labeling for individual bacterial cell detection and recovery.
abstract: A culture independent approach was developed for recovering individual bacterial cells out of communities from complex environments including soils and sediments where autofluorescent contaminants hinder the use of fluorescence based techniques. For that purpose fifty nanometer sized streptavidin-coated superparamagnetic nanoparticles were used to chemically bond biotin-functionalized plasmid DNA molecules. We show that micromagnets can efficiently trap magnetically labeled transformed Escherichia coli cells after these bacteria were subjected to electro-transformation by these nanoparticle-labeled plasmids. Among other applications, this method could extend the range of approaches developed to study DNA dissemination among environmental bacteria without requiring cultivability of recombinant strains or expression of heterologous genes in the new hosts.
authors.affiliation: Ecole Centrale de Lyon, CNRS UMR 5005, Laboratoire Ampère, F-69134 Écully, France. Electronic address: jeremy.piv@netcmail.com.
authors.forename: Jérémy
authors.initials: J
authors.lastname: Pivetal
authors.affiliation: Univ. Grenoble Alpes, Inst NEEL, F-38042 Grenoble, France; CNRS, Inst NEEL, F-38042 Grenoble, France. Electronic address: geta_ciuta@yahoo.com.
authors.forename: Georgeta
authors.initials: G
authors.lastname: Ciuta
authors.affiliation: Université de Lyon, Université Lyon 1, CNRS UMR 5005, Laboratoire Ampère, F-69622 Villeurbanne, France. Electronic address: marie.robin@univ-lyon1.fr.
authors.forename: Marie
authors.initials: M
authors.lastname: Frenea-Robin
authors.affiliation: Ecole Centrale de Lyon, CNRS UMR 5005, Laboratoire Ampère, F-69134 Écully, France. Electronic address: naoufel.haddour@ec-lyon.fr.
authors.forename: Naoufel
authors.initials: N
authors.lastname: Haddour
authors.affiliation: Univ. Grenoble Alpes, Inst NEEL, F-38042 Grenoble, France; CNRS, Inst NEEL, F-38042 Grenoble, France. Electronic address: nora.dempsey@neel.cnrs.fr.
authors.forename: Nora M
authors.initials: NM
authors.lastname: Dempsey
authors.affiliation: Univ Limoges, CNRS, SPCTS UMR 7513, 12 Rue Atlantis, F-87068 Limoges, France. Electronic address: frederic.dumas-bouchiat@unilim.fr.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Dumas-Bouchiat
authors.affiliation: Ecole Centrale de Lyon, CNRS UMR 5005, Laboratoire Ampère, F-69134 Écully, France. Electronic address: pascal.simonet@ec-lyon.fr.
authors.forename: Pascal
authors.initials: P
authors.lastname: Simonet
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D004247:DNA / Q000737:chemistry*; D004269:DNA, Bacterial / Q000737:chemistry*; D004783:Environmental Microbiology; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D010957:Plasmids / Q000737:chemistry*; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial; D058185:Magnetite Nanoparticles; D004247:DNA
keywords: Electro-transformation; Horizontal gene transfer; Magnetic DNA labeling; Magnetic bacterial cell labeling; Magnetic nanoparticles