title: Encapsulating non-human primate multipotent stromal cells in alginate via high voltage for cell-based therapies and cryopreservation.
abstract: Alginate cell-based therapy requires further development focused on clinical application. To assess engraftment, risk of mutations and therapeutic benefit studies should be performed in an appropriate non-human primate model, such as the common marmoset (Callithrix jacchus). In this work we encapsulated amnion derived multipotent stromal cells (MSCs) from Callithrix jacchus in defined size alginate beads using a high voltage technique. Our results indicate that i) alginate-cell mixing procedure and cell concentration do not affect the diameter of alginate beads, ii) encapsulation of high cell numbers (up to 10×106 cells/ml) can be performed in alginate beads utilizing high voltage and iii) high voltage (15-30 kV) does not alter the viability, proliferation and differentiation capacity of MSCs post-encapsulation compared with alginate encapsulated cells produced by the traditional air-flow method. The consistent results were obtained over the period of 7 days of encapsulated MSCs culture and after cryopreservation utilizing a slow cooling procedure (1 K/min). The results of this work show that high voltage encapsulation can further be maximized to develop cell-based therapies with alginate beads in a non-human primate model towards human application.
authors.affiliation: Institute for Multiphase Processes, Leibniz Universitaet Hannover, Hannover, Germany.
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Gryshkov
authors.affiliation: Institute for Multiphase Processes, Leibniz Universitaet Hannover, Hannover, Germany.
authors.forename: Denys
authors.initials: D
authors.lastname: Pogozhykh
authors.affiliation: Institute for Multiphase Processes, Leibniz Universitaet Hannover, Hannover, Germany.
authors.forename: Nicola
authors.initials: N
authors.lastname: Hofmann
authors.affiliation: Institute for Transfusion Medicine, Hannover Medical School, Hannover, Germany.
authors.forename: Olena
authors.initials: O
authors.lastname: Pogozhykh
authors.affiliation: Institute for Transfusion Medicine, Hannover Medical School, Hannover, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Mueller
authors.affiliation: Institute for Multiphase Processes, Leibniz Universitaet Hannover, Hannover, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Glasmacher
mesh_terms: D000464:Alginates; D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D064987:Cell- and Tissue-Based Therapy; D015925:Cryopreservation; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010641:Phenotype; D011323:Primates
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid