title: Rapid identification of novel antigens ofSalmonellaEnteritidis by microarray-based immunoscreening.
abstract: We report on an approach to rapidly screen thousands ofSalmonellaEnteritidis proteins with the goal of identifying novel immunodominant proteins. We used a microarray-based system that warrants high throughput and easy handling. Seven immunogenic candidates were selected after screening. Comparative analyses by ELISA and microarrays manifested their immunodominant character. The large repetitive protein (SEN4030) that plays a role as a putative adhesin in initial cell surface interaction and is highly specific toSalmonellais considered to be the most suitable protein for a diagnostic approach. The results further demonstrate that the strategy applied herein is convenient for specifically identifying immunogenic proteins of pathogenic microorganisms. Consequently, it enables a sound assessment of promising candidates for diagnostic applications and vaccine development. Moreover, the elucidation of immunogenic proteins may assist in unveiling unknown virulence-associated factors, thus furthering the understanding of the underlying pathogenicity ofSalmonellain general, and ofS.Enteritidis, one of the most frequently detected serovars of this pathogen, in particular. FigureThe microarray-based approach was aimed at identifying novel immunodominant proteins ofS.Enteritidis. Seven antigens were revealed by screening a cDNA expression library. SEN4030, a large repetitive protein specific for salmonella, is considered an optimal candidate for future applications.
authors.affiliation: Fraunhofer IBMT, Am Mühlenberg 13., 14476 Potsdam, Germany.
authors.forename: Lena
authors.initials: L
authors.lastname: Danckert
authors.affiliation: Fraunhofer IBMT, Am Mühlenberg 13., 14476 Potsdam, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Hoppe
authors.affiliation: Fraunhofer IBMT, Am Mühlenberg 13., 14476 Potsdam, Germany.
authors.forename: Frank F
authors.initials: FF
authors.lastname: Bier
authors.affiliation: Fraunhofer IBMT, Am Mühlenberg 13., 14476 Potsdam, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: von Nickisch-Rosenegk
keywords: Antigen detection; Microarray; Salmonella