title: Molecular characterization of Eurytrema coelomaticum in cattle from Paraná, Brazil.
abstract: This study investigated the occurrence of Eurytrema spp. in cattle by analysis of the partial 18S rRNA gene sequence. Trematodes from 44 bovine pancreas were collected and classified based on typical morphological features. PCR assay and sequence analyses of amplified products confirmed that the trematodes classified as Eurytrema coelomaticum were phylogenetically distinct from those identified as E. pancreaticum. The results of this study represent the first molecular characterization of E. coelomaticum within the Americas, and provide an efficient method to differentiate digenean trematodes of domestic animals.
authors.affiliation: School of Veterinary Medicine, Universidade Norte do Paraná, Arapongas, PR, Brazil.
authors.forename: Gustavo Freire
authors.initials: GF
authors.lastname: Figueira
authors.affiliation: Laboratory of Molecular Biology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina ? UEL, Londrina, PR, Brazil.
authors.forename: Victor Henrique Silva de
authors.initials: VH
authors.lastname: Oliveira
authors.affiliation: Laboratory of Parasitology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina ? UEL, PR, Brazil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Taroda
authors.affiliation: Laboratory of Molecular Biology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina ? UEL, Londrina, PR, Brazil.
authors.forename: Amauri Alcindo
authors.initials: AA
authors.lastname: Alfieri
authors.affiliation: Laboratory of Veterinary Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina ? UEL, PR, Brazil.
authors.forename: Selwyn Arlington
authors.initials: SA
authors.lastname: Headley
mesh_terms: D000818:Animals; D001938:Brazil; D002417:Cattle / Q000469:parasitology*; D004012:Dicrocoeliidae / Q000235:genetics* / Q000502:physiology; D012337:RNA, Ribosomal, 18S / Q000032:analysis*; D017423:Sequence Analysis, RNA
chemical_list: D012337:RNA, Ribosomal, 18S