title: Prevalence of subgingival Staphylococcus at periodontally healthy and diseased sites.
abstract: Staphylococci are considered members of the transient oral microbiota and are seldom isolated from the oral cavity. The aim of this study was to establish the prevalence of subgingival staphylococci in healthy and periodontal disease sites. Sterile endodontic paper points were used to isolate subgingival staphylococci in periodontally healthy and periodontally diseased sites in 30 adult subjects (n=540 sites). Staphylococcus spp were identified by an automated method and confirmed by conventional biochemical tests. All the samples were identified as coagulase-negative staphylococci. The results were analyzed using Mann-Whitney U, chi-square and Fisher's exact test at 5% significance level. A total of 86.7% of the subjects harbored these microorganisms in 11.7% of their periodontal sites. The most frequently isolated species was S. auricularis, which was isolated from 31.4% of the periodontal sites, followed by S. epidermidis, isolated from 21.4% of them. There was no statistically significant difference between the frequencies of these species isolated either from the healthy and the diseased sites (p>0.153). Although staphylococci are present in the subgingival environment and contribute to the pathogenic synergism involved in periodontal diseases, the results suggest that they do not participate directly in the pathogenesis of these diseases.
authors.affiliation: Department of Dentistry, UFRN - Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Bruna Rafaela Martins
authors.initials: BR
authors.lastname: dos Santos
authors.affiliation: Department of Dentistry, UFRN - Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Clarissa Favero
authors.initials: CF
authors.lastname: Demeda
authors.affiliation: UFRN - Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Eutália Elizabeth Novaes Ferreira
authors.initials: EE
authors.lastname: da Silva
authors.affiliation: Center of Clinical Pathology, Natal, RN, Brazil.
authors.forename: Maria Helena Marques Fonsecae
authors.initials: MH
authors.lastname: de Britto
authors.affiliation: Department of Dentistry, UFRN - Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Kenio Costa
authors.initials: KC
authors.lastname: Lima
authors.affiliation: UFRN - Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Maria Celeste Nunes
authors.initials: MC
authors.lastname: de Melo
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D006801:Humans; D010518:Periodontitis / Q000382:microbiology*; D010519:Periodontium / Q000382:microbiology*; D015995:Prevalence; D013210:Staphylococcus / Q000302:isolation & purification*