title: Rapid identification of a natural knockout allele of ARMADILLO REPEAT-CONTAINING KINESIN1 that causes root hair branching by mapping-by-sequencing.
abstract: In Arabidopsis (Arabidopsis thaliana), branched root hairs are an indicator of defects in root hair tip growth. Among 62 accessions, one accession (Heiligkreuztal2 [HKT2.4]) displayed branched root hairs, suggesting that this accession carries a mutation in a gene of importance for tip growth. We determined 200- to 300-kb mapping intervals using a mapping-by-sequencing approach of F2 pools from crossings of HKT2.4 with three different accessions. The intersection of these mapping intervals was 80 kb in size featuring not more than 36 HKT2.4-specific single nucleotide polymorphisms, only two of which changed the coding potential of genes. Among them, we identified the causative single nucleotide polymorphism changing a splicing site in ARMADILLO REPEAT-CONTAINING KINESIN1. The applied strategies have the potential to complement statistical methods in high-throughput phenotyping studies using different natural accessions to identify causative genes for distinct phenotypes represented by only one or a few accessions.
authors.affiliation: Botanical Institute (L.R., M.H., A.S.) and Cluster of Excellence on Plant Sciences (L.R., M.H.), University of Cologne, Cologne Biocenter, D-50674 Cologne, Germany; andDepartment for Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany (H.S., K.S.).
authors.forename: Louai
authors.initials: L
authors.lastname: Rishmawi
authors.affiliation: Botanical Institute (L.R., M.H., A.S.) and Cluster of Excellence on Plant Sciences (L.R., M.H.), University of Cologne, Cologne Biocenter, D-50674 Cologne, Germany; andDepartment for Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany (H.S., K.S.).
authors.forename: Hequan
authors.initials: H
authors.lastname: Sun
authors.affiliation: Botanical Institute (L.R., M.H., A.S.) and Cluster of Excellence on Plant Sciences (L.R., M.H.), University of Cologne, Cologne Biocenter, D-50674 Cologne, Germany; andDepartment for Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany (H.S., K.S.).
authors.forename: Korbinian
authors.initials: K
authors.lastname: Schneeberger
authors.affiliation: Botanical Institute (L.R., M.H., A.S.) and Cluster of Excellence on Plant Sciences (L.R., M.H.), University of Cologne, Cologne Biocenter, D-50674 Cologne, Germany; andDepartment for Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany (H.S., K.S.) martin.huelskamp@uni-koeln.de andrea.schrader@uni-koeln.de.
authors.forename: Martin
authors.initials: M
authors.lastname: Hülskamp
authors.affiliation: Botanical Institute (L.R., M.H., A.S.) and Cluster of Excellence on Plant Sciences (L.R., M.H.), University of Cologne, Cologne Biocenter, D-50674 Cologne, Germany; andDepartment for Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany (H.S., K.S.) martin.huelskamp@uni-koeln.de andrea.schrader@uni-koeln.de.
authors.forename: Andrea
authors.initials: A
authors.lastname: Schrader
mesh_terms: D000483:Alleles; D000818:Animals; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D051186:Armadillo Domain Proteins / Q000235:genetics* / Q000378:metabolism; D001135:Armadillos; D002874:Chromosome Mapping; D016547:Kinesins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development; D020641:Polymorphism, Single Nucleotide
chemical_list: D029681:Arabidopsis Proteins; D051186:Armadillo Domain Proteins; C529971:MRH2 protein, Arabidopsis; D016547:Kinesins