title: New procyanidin B3-human salivary protein complexes by mass spectrometry. Effect of salivary protein profile, tannin concentration, and time stability.
abstract: Several factors could influence the tannin-protein interaction such as the human salivary protein profile, the tannin tested, and the tannin/protein ratio. The goal of this study aims to study the effect of different salivas (A, B, and C) and different tannin concentrations (0.5 and 1 mg/mL) on the interaction process as well as the complex's stability over time. This study is focused on the identification of new procyanidin B3-human salivary protein complexes. Thus, 48 major B3-human salivary protein aggregates were identified regardless of the saliva and tannin concentration tested. A higher number of aggregates was found at lower tannin concentration. Moreover, the number of protein moieties involved in the aggregation process was higher when the tannin concentration was also higher. The selectivity of the different groups of proteins to bind tannin was also confirmed. It was also verified that the B3-human salivary protein complexes formed evolved over time.
authors.affiliation: Departamento de Quimica e Bioquimica, Faculdade de Ciências da Universidade do Porto , Rua Campo Alegre 687, 4169-007 Porto, Portugal.
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Perez-Gregorio
authors.forename: Nuno
authors.initials: N
authors.lastname: Mateus
authors.forename: Victor
authors.initials: V
authors.lastname: De Freitas
mesh_terms: D044946:Biflavonoids / Q000737:chemistry*; D002392:Catechin / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D044945:Proanthocyanidins / Q000737:chemistry*; D011485:Protein Binding; D055550:Protein Stability; D012463:Saliva / Q000737:chemistry; D012471:Salivary Proteins and Peptides / Q000737:chemistry*; D013634:Tannins / Q000032:analysis*; D013997:Time Factors
chemical_list: D044946:Biflavonoids; D044945:Proanthocyanidins; D012471:Salivary Proteins and Peptides; D013634:Tannins; C479581:procyanidin B3; D002392:Catechin
keywords: astringency; procyanidin B3; salivary proteins; tannin−protein binding