title: Nuclease-functionalized poly(styrene-b-isobutylene-b-styrene) surface with anti-infection and tissue integration bifunctions.
abstract: Hydrophobic thermoplastic elastomers, e.g., poly(styrene-b-isobutylene-b-styrene) (SIBS), have found various in vivo biomedical applications. It has long been recognized that biomaterials can be adversely affected by bacterial contamination and clinical infection. However, inhibiting bacterial colonization while simultaneously preserving or enhancing tissue-cell/material interactions is a great challenge. Herein, SIBS substrates were functionalized with nucleases under mild conditions, through polycarboxylate grafts as intermediate. It was demonstrated that the nuclease-modified SIBS could effectively prevent bacterial adhesion and biofilm formation. Cell adhesion assays confirmed that nuclease coatings generally had no negative effects on L929 cell adhesion, compared with the virgin SIBS reference. Therefore, the as-reported nuclease coating may present a promising approach to inhibit bacterial infection, while preserving tissue-cell integration on polymeric biomaterials.
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, People's Republic of China.
authors.forename: Shuaishuai
authors.initials: S
authors.lastname: Yuan
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.forename: Shifang
authors.initials: S
authors.lastname: Luan
authors.forename: Shunjie
authors.initials: S
authors.lastname: Yan
authors.forename: Wanling
authors.initials: W
authors.lastname: Zheng
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yin
mesh_terms: D000327:Adsorption; D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D001424:Bacterial Infections / Q000188:drug therapy*; D018441:Biofilms / Q000254:growth & development; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D002460:Cell Line; D003851:Deoxyribonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology; D005340:Fibrinogen / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology; D051379:Mice; D056951:Photoelectron Spectroscopy; D012260:Ribonucleases / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology; D013343:Styrenes / Q000494:pharmacology / Q000627:therapeutic use*; D013499:Surface Properties
chemical_list: D013343:Styrenes; C500040:poly(styrene-b-isobutylene-b-styrene); D005340:Fibrinogen; D003851:Deoxyribonucleases; D012260:Ribonucleases
keywords: antibacterial; cell adhesion; deoxyribonuclease (DNase); poly(styrene-b-isobutylene-b-styrene) (SIBS); ribonuclease (RNase)