title: Single-cell cloning and expansion of human induced pluripotent stem cells by a microfluidic culture device.
abstract: The microenvironment of cells, which includes basement proteins, shear stress, and extracellular stimuli, should be taken into consideration when examining physiological cell behavior. Although microfluidic devices allow cellular responses to be analyzed with ease at the single-cell level, few have been designed to recover cells. We herein demonstrated that a newly developed microfluidic device helped to improve culture conditions and establish a clonality-validated human pluripotent stem cell line after tracing its growth at the single-cell level. The device will be a helpful tool for capturing various cell types in the human body that have not yet been established in vitro.
authors.affiliation: Research and Development Division, ARKRAY, Inc., Kyoto 602-0008, Japan.
authors.forename: Taku
authors.initials: T
authors.lastname: Matsumura
authors.affiliation: Mechanical Engineering and Science, Faculty of Engineering, Kyoto University, Yoshida Honmachi, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Tatsumi
authors.affiliation: Research and Development Division, ARKRAY, Inc., Kyoto 602-0008, Japan.
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Noda
authors.affiliation: Research and Development Division, ARKRAY, Inc., Kyoto 602-0008, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Nakanishi
authors.affiliation: Research and Development Division, ARKRAY, Inc., Kyoto 602-0008, Japan.
authors.forename: Atsuhito
authors.initials: A
authors.lastname: Okonogi
authors.affiliation: Research and Development Division, ARKRAY, Inc., Kyoto 602-0008, Japan. Electronic address: hiranok@arkray.co.jp.
authors.forename: Kunio
authors.initials: K
authors.lastname: Hirano
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, Japan.
authors.forename: Liu
authors.initials: L
authors.lastname: Li
authors.affiliation: Research and Development Division, ARKRAY, Inc., Kyoto 602-0008, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Osumi
authors.affiliation: Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogo-in, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Tada
authors.affiliation: Micro Engineering, Faculty of Engineering, Kyoto University, Yoshida Honmachi, Sakyo-ku, Kyoto 606-8501, Japan. Electronic address: Kotera_hide@me.kyoto-u.ac.jp.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kotera
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation; D049109:Cell Proliferation; D002999:Clone Cells / Q000166:cytology / Q000378:metabolism; D003198:Computer Simulation; D004867:Equipment Design; D020869:Gene Expression Profiling; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D046210:Microfluidic Analytical Techniques; D008954:Models, Biological; D055153:Stem Cell Niche
keywords: Culture conditions; Human induced pluripotent stem cells; Microenvironment; Microfluidic culture; Single-cell cloning