title: Expression of avian influenza haemagglutinin (H5) and chicken interleukin 2 (chIL-2) under control of the ptcB promoter in Lactococcus lactis.
abstract: Gram-positive and nonpathogenic lactic acid bacteria (LAB) are considered to be promising candidates for the development of new, safe systems of heterologous protein expression. Recombinant LAB has been shown to induce specific local and systemic immune response against selected pathogens, and could be a good alternative to classical attenuated carriers. The main goal of our study was to express the avian influenza haemagglutinin (H5) and chicken interleukin 2 (chIL-2) in Lactococcus lactis. Results of this study were anticipated to lead to construction of lactococcal strain(s) with potential vaccine properties against the avian influenza A (H5N1) virus. Expression of the cloned H5 gene, its His-tagged variant and chIL-2 gene, under the control of the ptcB gene promoter was attested by RT-PCR on transcriptional level and Western or dot blot analysis on translational level, demonstrating that system can be an attractive solution for production of heterologous proteins. The results of the preliminary animal trial conducted in mice are a promising step toward development of a vaccine against avian bird flu using Lactococcus lactis cells as antigen carriers.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Szatraj
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Agnieszka K
authors.initials: AK
authors.lastname: Szczepankowska
authors.affiliation: Institute of Biotechnology and Antibiotics, Department of Bioengineering, Warsaw, Poland.
authors.forename: Violetta
authors.initials: V
authors.lastname: Sączyńska
authors.affiliation: Institute of Biotechnology and Antibiotics, Department of Bioengineering, Warsaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Florys
authors.affiliation: University of Gdansk and Medical University of Gdańsk, Intercollegiate Faculty of Biotechnology, Department of Recombinant Vaccines, Gdańsk, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Gromadzka
authors.affiliation: University of Gdansk and Medical University of Gdańsk, Intercollegiate Faculty of Biotechnology, Department of Recombinant Vaccines, Gdańsk, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Łepek
authors.affiliation: Institute of Biotechnology and Antibiotics, Department of Bioengineering, Warsaw, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Płucienniczak
authors.affiliation: University of Gdańsk, Institute of Biotechnology, Department of Molecular Virology, Gdańsk, Poland.
authors.forename: Bogusław
authors.initials: B
authors.lastname: Szewczyk
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Zagórski-Ostoja
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Bardowski
mesh_terms: D000818:Animals; D002645:Chickens; D005822:Genetic Vectors; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000096:biosynthesis* / Q000235:genetics; D053124:Influenza A Virus, H5N1 Subtype / Q000737:chemistry / Q000235:genetics*; D007252:Influenza Vaccines; D005585:Influenza in Birds / Q000517:prevention & control*; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics; D013294:Lactococcus lactis / Q000235:genetics*; D051379:Mice; D011401:Promoter Regions, Genetic
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D007376:Interleukin-2; C109963:hemagglutinin, avian influenza A virus