title: Serum protein profiling of adults and children with Crohn disease.
abstract: OBJECTIVE
  Crohn disease (CD) and ulcerative colitis (UC), known collectively as inflammatory bowel diseases (IBDs), are chronic immunoinflammatory pathologies of unknown aetiology. Despite the frequent use of biomarkers in medical practice, there is a relative lack of information regarding validated paediatric biomarkers for IBD. Furthermore, biomarkers proved to be efficacious in adults are frequently extrapolated to the paediatric clinical setting without considering that the pathogenesis of many diseases is distinctly different in children. In the present study, proteomics technology was used to monitor differences in protein expression among adult and young patients with CD, identify a panel of candidate protein biomarkers that may be used to improve prognostic-diagnostic accuracy, and advance paediatric medical care.
  
  
  METHODS
  Male and female serum samples from 12 adults and 12 children with active CD were subjected to 2-dimensional gel electrophoresis. Following the relative quantitation of protein spots exhibiting a differential expression between the 2 groups by densitometry, the spots were further characterized by matrix-assisted laser desorption tandem time-of-flight mass spectrometer. The results were confirmed by Western blot analysis.
  
  
  RESULTS
  Clusterin was found to be significantly overexpressed in adults with CD, whereas ceruloplasmin and apolipoprotein B-100 were found to be significantly overexpressed in children, indicating that the expression of these proteins may be implicated in the onset or progression of CD in these 2 subgroups of patients.
  
  
  CONCLUSIONS
  Interestingly, we found a differential expression of several proteins in adults versus paediatric patients with CD. Undoubtedly, future experiments using a larger cohort of patients with CD are needed to evaluate the relevance of our preliminary findings.
authors.affiliation: *Department of Basic Medical Sciences, Laboratory of Biology, School of Medicine, University of Athens †Proteomics Research Unit, Biomedical Research Foundation of the Academy of Athens (IIBEAA) ‡Gastroenterology Unit, 2 Department of Surgery, "Aretaieio" University Hospital," Athens §First Propaedeutic Surgical Department, Hippocration University Hospital, University of Athens, Athens, Greece ||Department of Biochemistry and Cancer Biology, Meharry Medical College School of Medicine, Nashville, TN.
authors.forename: Anna
authors.initials: A
authors.lastname: Vaiopoulou
authors.forename: Maria
authors.initials: M
authors.lastname: Gazouli
authors.forename: Aggeliki
authors.initials: A
authors.lastname: Papadopoulou
authors.forename: Athanassios K
authors.initials: AK
authors.lastname: Anagnostopoulos
authors.forename: George
authors.initials: G
authors.lastname: Karamanolis
authors.forename: George E
authors.initials: GE
authors.lastname: Theodoropoulos
authors.forename: Amosy
authors.initials: A
authors.lastname: M'Koma
authors.forename: George T
authors.initials: GT
authors.lastname: Tsangaris
mesh_terms: D000328:Adult; D017668:Age of Onset; D053299:Apolipoprotein B-100 / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D015415:Biomarkers / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D001798:Blood Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D015153:Blotting, Western; D002570:Ceruloplasmin / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D002648:Child; D051152:Clusterin / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D003424:Crohn Disease / Q000097:blood* / Q000453:epidemiology / Q000503:physiopathology; D005260:Female; D006115:Greece / Q000453:epidemiology; D006801:Humans; D008297:Male; D010449:Peptide Mapping; D040901:Proteomics / Q000379:methods; D012720:Severity of Illness Index; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: C569471:APOB protein, human; D053299:Apolipoprotein B-100; D015415:Biomarkers; D001798:Blood Proteins; C495242:CLU protein, human; D051152:Clusterin; D002570:Ceruloplasmin