title: Tissue perfusion in neonates undergoing open-heart surgery using autologous umbilical cord blood or donor blood components.
abstract: BACKGROUND
  This study evaluates the hemoglobin-oxygen relationship and tissue perfusion during cardiopulmonary bypass (CPB) in neonates undergoing open-heart surgery using autologous umbilical cord blood or donor blood components.
  
  
  METHODS
  We compared perioperative hematocrit (Hct), fetal hemoglobin (HbF), p(50)O(2), pH, pCO(2), serum lactate, duration of mechanical ventilation and intensive care unit (ICU) length of stay in neonates undergoing open-heart surgery using autologous umbilical cord blood (Group I, N=45) or donor blood components (Group II, N=65). The groups were similar with respect to diagnosis, weight, type of procedure, duration of CPB and duration of myocardial ischemia.
  
  
  RESULTS
  Mean p(50)O(2) was significantly lower in Group I during CPB (19.7 vs. 22.3 mmHg, p=0.004) and at the end of CPB (20.1 vs. 22.8 mmHg, p=0.003). Median peak lactate during CPB was higher in Group I (4.8 vs. 2.2 mmol/l, p<0.001). Carbon dioxide tension was identified as an independent predictor of higher p(50)O(2) during CPB in Group I (β=0.88, p=0.002), but not Group II. Bodyweight, Hct, duration of CPB, pre-CPB lactate level and pCO(2) affected peak lactate level during CPB. Although mean duration of ventilation was longer in Group II (mean 51 vs. 43, p=0.004), the groups experienced similar duration of ICU stay (5.8 vs. 5.9 days, p=0.280).
  
  
  CONCLUSIONS
  Despite the fact that the oxyhemoglobin dissociation curve is shifted leftward in patients who receive autologous umbilical cord blood, tissue oxygen delivery appears to be preserved in neonates who undergo open-heart surgery using autologous umbilical cord blood.
authors.affiliation: Perfusiology, Ukrainian Children's Cardiac Center, Kyiv, Ukraine kchasovskiy82@gmail.com.
authors.forename: K
authors.initials: K
authors.lastname: Chasovskyi
authors.affiliation: Surgery, Ukrainian Children's Cardiac Center, Kyiv, Ukraine.
authors.forename: O
authors.initials: O
authors.lastname: Fedevych
authors.affiliation: Surgery, Seattle Children's Hospital, USA.
authors.forename: D M
authors.initials: DM
authors.lastname: McMullan
authors.affiliation: Surgery, Ukrainian Children's Cardiac Center, Kyiv, Ukraine.
authors.forename: Y
authors.initials: Y
authors.lastname: Mykychak
authors.affiliation: Immunology, Ukrainian Children's Cardiac Center, Kyiv, Ukraine.
authors.forename: G
authors.initials: G
authors.lastname: Vorobiova
authors.affiliation: Anesthesiology, Ukrainian Children's Cardiac Center, Kyiv, Ukraine.
authors.forename: V
authors.initials: V
authors.lastname: Zhovnir
authors.affiliation: Surgery, Ukrainian Children's Cardiac Center, Kyiv, Ukraine.
authors.forename: I
authors.initials: I
authors.lastname: Yemets
mesh_terms: D016913:Blood Component Transfusion; D001782:Blood Donors; D001804:Blood Transfusion, Autologous; D002315:Cardiopulmonary Bypass / Q000379:methods*; D005260:Female; D005312:Fetal Blood; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D017202:Myocardial Ischemia / Q000097:blood / Q000601:surgery
keywords: CHD; arterial switch operation; autologous umbilical cord blood; cardiopulmonary bypass; hemoglobin-oxygen relationship