title: The protective effect of ozone oxidative preconditioning against hypoxia/reoxygenation injury in rat kidney cells.
abstract: Abstract Ozone (O3) has been viewed as a novel treatment for different diseases in these years and oxidative stress and apoptosis play a key role in the pathogenesis of kidney diseases including renal ischemia and reperfusion (I/R). In the present study, we investigated the role of ozone oxidative preconditioning (OzoneOP) in attenuating oxidative stress and apoptosis in a hypoxia/reoxygenation (H/R) injury model using rat kidney cells. We induced H/R injury in kidney cells treated with or without OzoneOP. Oxidative stress parameters such as superoxide dismutase (SOD), malondialdehyde (MDA) and lactate dehydrogenase (LDH) were determined, as well as some apoptotic proteins. We observed that oxidative stress and apoptosis were increased in H/R group compared to OzoneOP group; however, these changes were significantly decreased by the treatment with OzoneOP. We concluded that OzoneOP can protect the kidney cells against H/R injury and its mechanism may be through the reduction of oxidative stress and apoptosis.
authors.affiliation: Department of Urology, Renmin Hospital of Wuhan University , Wuhan, Hubei Province , P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Xiu-Heng
authors.initials: XH
authors.lastname: Liu
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Chen
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Weng
authors.forename: Tao
authors.initials: T
authors.lastname: Qiu
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D004195:Disease Models, Animal; D000860:Hypoxia / Q000188:drug therapy*; D007150:Immunohistochemistry; D019194:Ischemic Preconditioning / Q000379:methods*; D007674:Kidney Diseases / Q000188:drug therapy* / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D010083:Oxidants, Photochemical / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D010126:Ozone / Q000494:pharmacology*; D051381:Rats; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D010083:Oxidants, Photochemical; D008315:Malondialdehyde; D010126:Ozone; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase
keywords: Apoptosis; hypoxia and reoxygenation; oxidative stress; ozone oxidative preconditioning; rat