title: Propranolol attenuates calorie restriction- and high calorie diet-induced bone marrow adiposity.
abstract: We investigated the effects of β-adrenergic activation on bone marrow adiposity and on adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). C57BL/6 mice were subjected to a control (CON), high calorie (HIGH) or low calorie (LOW) diet for 12 weeks. In each group, mice were treated with vehicle (VEH) or propranolol. The number of adipocytes per area bone marrow was increased in LOWVEH and HIGHVEH mice compared with CONVEH mice, which was attenuated by propranolol. Isoproterenol increased lipid droplet accumulation and adipogenic marker gene expression in 3T3-L1 preadipocytes and mouse BMSCs, which were blocked by propranolol. Conditioned medium obtained from MC3T3-E1 osteoblasts suppressed adipogenic differentiation of 3T3-L1 cells, which was significantly attenuated by treatment of MC3T3-E1 cells with isoproterenol. These data suggest that β-adrenergic activation enhances bone marrow adipogenesis via direct stimulation of BMSCs adipogenesis and indirect inhibition of osteoblast anti-adipogenic potential.
authors.affiliation: Department of Pharmacology and Research Institute of Oral Science, College of Dentistry, Gangneung-Wonju National University, Gangwon 210-702, Korea.
authors.forename: Kyunghwa
authors.initials: K
authors.lastname: Baek
authors.affiliation: Department of Molecular Genetics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul 110-744, Korea.
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Park
authors.affiliation: Department of Molecular Genetics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul 110-744, Korea.
authors.forename: Hyo Rin
authors.initials: HR
authors.lastname: Hwang
authors.affiliation: Department of Molecular Genetics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul 110-744, Korea.
authors.forename: Jeong-Hwa
authors.initials: JH
authors.lastname: Baek
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects; D050154:Adiposity / Q000187:drug effects*; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000502:physiology*; D031204:Caloric Restriction; D004032:Diet; D015536:Down-Regulation / Q000187:drug effects; D007545:Isoproterenol / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D011433:Propranolol / Q000494:pharmacology*
chemical_list: D000319:Adrenergic beta-Antagonists; D011433:Propranolol; D007545:Isoproterenol