title: Mycelial growth rate and macro- and micromorphological characteristics of medicinal species of genus Ganoderma (higher Basidiomycetes) from Iran.
abstract: Mycelial growth rate is a distinguishing quality that demonstrates continuous variation in different isolates collected from various hosts and locations. The objectives of this research were (1) to reinvestigate the previous identification of Iranian species, and (2) to recognize the best native isolate(s) for cultivation of different Ganoderma species. Of 78 samples collected from different hosts and sites, only 43 mycelia could be purified and examined for further study. Growth rate (GR; Δd/Δt) and growth coefficient (GC; dgh/t) were analyzed by growing isolate culture on 2% malt-extract agar medium (pH 5.5) incubated at 25°C. Macro- and micromorphological studies on mycelia and fruiting bodies such as basidiospore and cutis microcharacters as well as fruiting body quality were used for precise identification. Results revealed that samples belonged to 4 species: G. lucidum, G. applanatum, G. resinaceum, and G. australe. Among all samples, the isolate morphologically identified as G. applanatum showed the best GR (12 mm/day) and good GC (128 mm/day), followed by the 2 other isolates identified as G. resinaceum (GRs and GCs of 11 and 55 mm/day and 10.9 and 43.6 mm/day, respectively).
authors.affiliation: Bioscience Faculty, Shahid Beheshti University, Evin, Tehran, Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Keypour
authors.affiliation: Bioscience Faculty, Shahid Beheshti University, Evin, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Riahi
authors.affiliation: Department of plant Pathology, College of Agriculture, Tarbiat Modares University, Tehran, Iran.
authors.forename: Naser
authors.initials: N
authors.lastname: Safaie
authors.affiliation: Agriculture and Natural Research Center of Mazandaran, Passand Forest and Rangeland Research Station, Behshahr, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Borhani
mesh_terms: D003470:Culture Media / Q000737:chemistry; D048690:Fruiting Bodies, Fungal / Q000166:cytology* / Q000254:growth & development* / Q000302:isolation & purification; D032902:Ganoderma / Q000145:classification* / Q000166:cytology / Q000254:growth & development* / Q000302:isolation & purification; D007492:Iran; D025282:Mycelium / Q000166:cytology* / Q000254:growth & development* / Q000302:isolation & purification; D013696:Temperature
chemical_list: D003470:Culture Media