title: A novel wheat-Dasypyrum breviaristatum substitution line with stripe rust resistance.
abstract: The introduction of genetic variation from wild and cultivated Triticeae species has been a long-standing approach for wheat improvement. Dasypyrum breviaristatum species harbor novel and agronomically important genes for resistance against multi-fungal diseases. The development of new wheat-D. breviaristatum introgression lines offers chances for the identification of stripe rust resistance gene(s). A wheat line, D11-5, was selected from a cross between wheat line MY11 and wheat-D. breviaristatum partial amphiploid TDH-2. It was characterized by FISH and PCR-based molecular markers. Chromosome counting revealed that the D11-5 line shows a hexaploid set of 2n = 6x = 42 chromosomes. FISH analysis using the Dasypyrum repetitive sequence pDb12H as a probe demonstrated that D11-5 contained a pair of D. breviaristatum chromosomes, while FISH with wheat D-genomic repetitive sequences revealed that the chromosome 2D was absent in D11-5. The functional molecular markers confirmed that the introduced D. breviaristatum chromosomes belong to the homoeologous group 2, indicating that D11-5 was a 2V(b) (2D) disomic substitution line. Field resistance showed that the introduced D. breviaristatum chromosomes 2V(b) were responsible for the stripe rust resistance at the adult plant stage. FISH, C-banding, and PCR-based molecular marker analysis indicated that the chromosome 2V(b) of D. breviaristatum was completely different from the chromosome 2V of D. villosum. The identified wheat-D. breviaristatum chromosome substitution line D11-5 may be applied to produce agronomically desirable stripe rust resistance germplasm.
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
authors.forename: Guang-Rong
authors.initials: GR
authors.lastname: Li
authors.forename: Jin-Mei
authors.initials: JM
authors.lastname: Zhao
authors.forename: Dong-Hai
authors.initials: DH
authors.lastname: Li
authors.forename: En-Nian
authors.initials: EN
authors.lastname: Yang
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Huang
authors.forename: Cheng
authors.initials: C
authors.lastname: Liu
authors.forename: Zu-Jun
authors.initials: ZJ
authors.lastname: Yang
mesh_terms: D001203:Ascomycota / Q000502:physiology; D001483:Base Sequence; D001487:Basidiomycota / Q000502:physiology; D032461:Chromosomes, Plant / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D006824:Hybridization, Genetic; D017404:In Situ Hybridization, Fluorescence; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology*; D011003:Ploidies; D014908:Triticum / Q000235:genetics* / Q000276:immunology
chemical_list: D018744:DNA, Plant