title: Maribacter thermophilus sp. nov., isolated from an algal bloom in an intertidal zone, and emended description of the genus Maribacter.
abstract: A novel facultatively anaerobic, Gram-stain-negative bacterium, designated strain HT7-2(T), was isolated from Ulva prolifera collected from the intertidal zone of Qingdao sea area, China, during its bloom. Cells were rod-shaped (1.9-3.5×0.4-0.6 µm), non-sporulating and motile by gliding. Strain HT7-2(T) was able to grow at 4-50 °C (optimum 40-42 °C), pH 5.5-8.5 (optimum pH 7.0), 0-8 % (w/v) NaCl (optimum 2-3 %) and 0.5-10 % (w/v) sea salts (optimum 2.5 %). The genomic DNA G+C content was 38.8 mol%. The phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HT7-2(T) belonged to the genus Maribacter with sequence similarity values of 94.5-96.6 %, and was most closely related to Maribacter aestuarii GY20(T) (96.6%). Chemotaxonomic analysis showed that the main isoprenoid quinone was MK-6 and the major fatty acids were iso-C15:0 and unknown equivalent chain-length 13.565. The polar lipids of strain HT7-2(T) consisted of one phosphatidylethanolamine, four unidentified lipids and one unidentified aminolipid. On the basis of the phenotypic, phylogenetic and chemotaxonomic characteristics, strain HT7-2(T) ( =CGMCC 1.12207(T) =JCM 18466(T)) is concluded to represent a novel species of the genus Maribacter, for which the name Maribacter thermophilus sp. nov. is proposed. An emended description of the genus Maribacter is also proposed.
authors.affiliation: College of Life Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.affiliation: College of Life Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Qi-Qi
authors.initials: QQ
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ren
authors.affiliation: College of Life Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Wen-Wu
authors.initials: WW
authors.lastname: Zhang
authors.affiliation: Ocean Research Center of Zhoushan, Zhejiang University, Zhoushan 316021, PR China.
authors.forename: Gang
authors.initials: G
authors.lastname: Zheng
authors.affiliation: College of Life Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Cong
authors.initials: C
authors.lastname: Sun
authors.affiliation: College of Life Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Pan
authors.affiliation: College of Life Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Xu-Fen
authors.initials: XF
authors.lastname: Zhu
authors.affiliation: School of Forestry and Biotechnology, Zhejiang Agriculture & Forestry University, Linan 311300, PR China.
authors.forename: Xin-Qi
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005068:Eutrophication; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D044445:Ulva / Q000382:microbiology*; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6