title: Human urine-derived stem cells in combination with polycaprolactone/gelatin nanofibrous membranes enhance wound healing by promoting angiogenesis.
abstract: BACKGROUND
  Despite advancements in wound healing techniques and devices, new treatments are needed to improve therapeutic outcomes. This study aimed to evaluate the potential use of a new biomaterial engineered from human urine-derived stem cells (USCs) and polycaprolactone/gelatin (PCL/GT) for wound healing.
  
  
  METHODS
  USCs were isolated from healthy individuals. To fabricate PCL/GT composite meshes, twin-nozzle electrospinning were used to spin the PCL and gelatin solutions in normal organic solvents. The morphologies and hydrophilicity properties of PCL/GT membranes were investigated. After USCs were seeded onto a PCL/GT, cell adhesion, morphology, proliferation, and cytotoxicity were examined. Then, USCs were seeded on a PCL/GT blend nanofibrous membrane and transplanted into rabbit full-thickness skin defects for wound repair. Finally, the effect of USCs condition medium on proliferation, migration, and tube formation of human umbilical vein endothelial cells (HUVECs) were performed in vitro.
  
  
  RESULTS
  USCs were successfully isolated from urine samples and expressed specific mesenchymal stem cells markers and could differentiate into osteoblasts, adipocytes, and chondrocytes. PCL/GT membrane has suitable mechanical properties similar with skin tissue and has good biocompatibility. USCs-PCL/GT significantly enhanced the healing of full-thickness skin wounds in rabbits compared to wounds treated with PCL/GT membrane alone or untreated wounds. USCs-PCL/GT-treated wounds closed much faster, with increased re-epithelialization, collagen formation, and angiogenesis. Moreover, USCs could secrete VEGF and TGF-β1, and USC-conditioned medium enhanced the migration, proliferation, and tube formation of endothelial cells.
  
  
  CONCLUSIONS
  USCs in combination with PCL/GT significantly prompted the healing of full-thickness skin wounds in rabbits. USCs based therapy provides a novel strategy for accelerating wound closure and promoting angiogenesis.
authors.affiliation: Institute of Microsurgery on Extremities, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, 200233, China. 18837124@qq.com.|Department of Clinical Laboratory, Pu' ai Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. 18837124@qq.com.
authors.forename: Yinxin
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Institute of Microsurgery on Extremities, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, 200233, China. junjie_guan@126.com.
authors.forename: Junjie
authors.initials: J
authors.lastname: Guan
authors.affiliation: Institute of Microsurgery on Extremities, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, 200233, China. 506763093@qq.com.
authors.forename: Shangchun
authors.initials: S
authors.lastname: Guo
authors.affiliation: Jiangxi Origin Bio-TECH Co. LTD, Nanchang, 330006, China. 15800616579@126.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.affiliation: Institute of Microsurgery on Extremities, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, 200233, China. niuxin999@163.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Niu
authors.affiliation: Jiangxi Origin Bio-TECH Co. LTD, Nanchang, 330006, China. 119410561@qq.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Institute of Microsurgery on Extremities, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, 200233, China. zhangcq@sjtu.edu.cn.
authors.forename: Changqing
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Jiangxi Origin Bio-TECH Co. LTD, Nanchang, 330006, China. niehr@iccas.ac.cn.|Jiangxi Chuanqi pharmaceutical Co., LTD, Nanchang, 330039, China. niehr@iccas.ac.cn.
authors.forename: Huarong
authors.initials: H
authors.lastname: Nie
authors.affiliation: Institute of Microsurgery on Extremities, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, 200233, China. wangy63cn@126.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000328:Adult; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005780:Gelatin / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011091:Polyesters / Q000494:pharmacology*; D011817:Rabbits; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D014556:Urine / Q000166:cytology*; D014945:Wound Healing / Q000187:drug effects*; D055815:Young Adult
chemical_list: D001672:Biocompatible Materials; D017077:Culture Media, Conditioned; D008567:Membranes, Artificial; D011091:Polyesters; C016240:polycaprolactone; D005780:Gelatin