title: Adipocyte Derived Hormones Gene Expression, Resistin and Visfatin, in AGS Gastric Cancer Cell Line.
abstract: BACKGROUND
  Adipose tissue has characteristics of an endocrine organ which releases a number of adipocyte-specific factors, known as adipocytokines. It has recently suggested that adipocytokines might play a role in pathogenesis and progression of certain cancers, especially in gastric cancer. This study has managed to investigate endogenous and/or exogenous expression of Visfatin and Resistin in gastric cancer cell line.
  
  
  METHODS
  Cell culture and semi-quantitative reverse transcription polymerase chain reaction has performed to measure mRNA and protein expression of Resistin and Visfatin in gastric cancer cell lines. ELISA test has performed for cell lysate and supernatant of cell culture to measure Resistin and Visfatin protein expression and secretion.
  
  
  RESULTS
  Human gastric cancer cell line (AGS cell line) has found to express Visfatin mRNA and protein but Resistin mRNA and protein has not expressed.
  
  
  CONCLUSIONS
  Visfatin has expressed endogenously in AGS human gastric cancer cells. Conversely Resistin has no expression. The results of this study has suggested that expression of adipocytokine proteins in real samples, could be a biomarker for gastric cancer.
authors.affiliation: Dept. of Clinical Biochemistry, Tabriz University of Medical Sciences, Tabriz, Iran ; Dept. of Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences,Tehran, Iran.
authors.forename: Masumeh
authors.initials: M
authors.lastname: Gorgian Mohammadi
authors.affiliation: Dept. of Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences,Tehran, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Hedayati
authors.affiliation: Dept. of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Nosratollah
authors.initials: N
authors.lastname: Zarghami
authors.affiliation: Dept. of Clinical Biochemistry, Tabriz University of Medical Sciences, Tabriz, Iran ; Dept. of Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences,Tehran, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Ghaemmaghami
authors.affiliation: Dept. of Medical Genetic, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Mohaddes
keywords: AGS cell line; Gastric cancer; Nampt; Resistin; Visfatin