title: Interleukin 1β (+3954, -511 and -31) polymorphism in chronic periodontitis patients from North India.
abstract: OBJECTIVE
  Several studies have implicated the role of interleukin-1 in various chronic diseases including periodontitis. The present study was carried out with an aim to evaluate the role of interleukin 1β polymorphisms, namely +3954C/T, -511C/T and -31T/C, in the development of chronic periodontitis.
  
  
  METHODS
  Twenty-nine chronic periodontitis patients and 31 healthy controls of North Indian origin from Chhattisgarh were recruited for the study. The genotypes for the three variants were determined using the PCR-RFLP technique and the strength of association between genotypes and periodontitis was determined by odds ratio with 95% confidence interval (CI) and chi-square analysis.
  
  
  RESULTS
  Analysis for the +3954 allelic and genotypic frequencies of the polymorphism revealed a significant difference in the CT genotype between periodontitits patients and controls (p = 0.03). A significant difference was also observed in the allelic frequencies between the two groups (p = 0.02). For the -511 site, TT genotype revealed a significant association with the disease (p = 0.01). A significant association was also found following the co-dominant model (p = 0.007). However, the -31 polymorphism revealed no significant difference between patients and controls.
  
  
  CONCLUSIONS
  In conclusion, the present study suggests a strong association of the TT genotype of -511 and CT genotype of +3954 variant of interleukin 1β with chronic periodontitis. However, the -31 variant did not show a significant association with the disease.
authors.affiliation: Chhattisgarh Dental College & Research Institute , Rajnandgaon, Chhattisgarh , India.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Amirisetty
authors.forename: Ritu Prabha
authors.initials: RP
authors.lastname: Patel
authors.forename: Satrupa
authors.initials: S
authors.lastname: Das
authors.forename: Jitendra
authors.initials: J
authors.lastname: Saraf
authors.forename: Akka
authors.initials: A
authors.lastname: Jyothy
authors.forename: Anjana
authors.initials: A
authors.lastname: Munshi
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000235:genetics / Q000276:immunology*; D003596:Cytosine; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007194:India; D053583:Interleukin-1beta / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D013941:Thymine
chemical_list: D053583:Interleukin-1beta; D003596:Cytosine; D013941:Thymine
keywords: chronic periodontitis; genetics; interleukin-1β; polymorphism