title: Microbacterium petrolearium sp. nov., isolated from an oil-contaminated water sample.
abstract: A Gram-staining-positive, rod-shaped, aerobic bacterium, designated strain LAM0410(T), was isolated from an oil-contaminated water sample from the Dagang Oilfield, PR China. The temperature and pH ranges for growth were 4 °C to 50 °C and pH 4.4 to pH 12.0, respectively. The strain did not need NaCl for growth, but could tolerate a concentration of up to 12 % (w/v). Cell wall hydrolysates from the isolate showed that the diamino acid was ornithine. The cell wall sugars contained ribose and galactose. The glycan moiety of the cell wall contained N-glycolyl residues. The major respiratory quinones were MK-10, MK-11 and MK-12. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and nine unknown glycolipids. The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. 16S rRNA gene sequence analysis indicated that the isolate belonged to the genus Microbacterium and was closely related to Microbacterium sediminis MCCC 1A06153(T) and Microbacterium murale DSM 22178(T) with 97.5 % and 97.4 % sequence similarities, respectively; while the DNA-DNA hybridization values were 33.1±3.4 % and 21.8±1.6 %, respectively. The DNA G+C content was 67.4 mol%, as determined by the Tm method. Based on its phenotypic and genotypic properties, strain LAM0410(T) represents a novel species of the genus Microbacterium, for which the name Microbacterium petrolearium sp. nov. is proposed; the type strain is LAM0410(T) ( = ACCC 00719(T) = JCM 19612(T)).
authors.affiliation: Earth Environmental and Water Resources College, Yangtze University, Jingzhou 434023, PR China.|Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Earth Environmental and Water Resources College, Yangtze University, Jingzhou 434023, PR China.
authors.forename: Tingsheng
authors.initials: T
authors.lastname: Xiang
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Song
authors.affiliation: Agricultural Engineering Institute, Chongqing Academy of Agricultural Sciences, Chongqing 401329, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhai
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Yanting
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Bingqiang
authors.initials: B
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Ruan
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D061329:Oil and Gas Fields / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000737:chemistry; D014871:Water Microbiology; D014877:Water Pollution, Chemical
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2