title: Global transcriptional profiling of a cold-tolerant rice variety under moderate cold stress reveals different cold stress response mechanisms.
abstract: Gene expression profiling under severe cold stress (4°C) has been conducted in plants including rice. However, rice seedlings are frequently exposed to milder cold stresses under natural environments. To understand the responses of rice to milder cold stress, a moderately low temperature (8°C) was used for cold treatment prior to genome-wide profiling of gene expression in a cold-tolerant japonica variety, Lijiangxintuanheigu (LTH). A total of 5557 differentially expressed genes (DEGs) were found at four time points during moderate cold stress. Both the DEGs and differentially expressed transcription factor genes were clustered into two groups based on their expression, suggesting a two-phase response to cold stress and a determinative role of transcription factors in the regulation of stress response. The induction of OsDREB2A under cold stress is reported for the first time in this study. Among the anti-oxidant enzyme genes, glutathione peroxidase (GPX) and glutathione S-transferase (GST) were upregulated, suggesting that the glutathione system may serve as the main reactive oxygen species (ROS) scavenger in LTH. Changes in expression of genes in signal transduction pathways for auxin, abscisic acid (ABA) and salicylic acid (SA) imply their involvement in cold stress responses. The induction of ABA response genes and detection of enriched cis-elements in DEGs suggest that ABA signaling pathway plays a dominant role in the cold stress response. Our results suggest that rice responses to cold stress vary with the specific temperature imposed and the rice genotype.
authors.affiliation: Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Guangdong Provincial Key Laboratory of New Technology in Rice Breeding, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
authors.forename: Junliang
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Guangdong Provincial Key Laboratory of New Technology in Rice Breeding, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
authors.forename: Shaohong
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Guangdong Provincial Key Laboratory of New Technology in Rice Breeding, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
authors.forename: Tifeng
authors.initials: T
authors.lastname: Yang
authors.affiliation: Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Guangdong Provincial Key Laboratory of New Technology in Rice Breeding, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
authors.forename: Zichong
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Guangdong Provincial Key Laboratory of New Technology in Rice Breeding, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
authors.forename: Zhanghui
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Guangdong Provincial Key Laboratory of New Technology in Rice Breeding, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Guangdong Provincial Key Laboratory of New Technology in Rice Breeding, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Bioagricultural Sciences and Pest Management and Program in Plant Molecular Biology, Colorado State University, Fort Collins, CO, 80523-1177, USA.
authors.forename: Jan
authors.initials: J
authors.lastname: Leach
authors.affiliation: Plant Breeding, Genetics, and Biotechnology, International Rice Research Institute, Laguna, 4031, Philippines.
authors.forename: Hei
authors.initials: H
authors.lastname: Leung
authors.affiliation: Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.|Guangdong Provincial Key Laboratory of New Technology in Rice Breeding, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D016000:Cluster Analysis; D003080:Cold Temperature; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000145:classification / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins