title: Inhibition of ROS production through mitochondria-targeted antioxidant and mitochondrial uncoupling increases post-thaw sperm viability in yellow catfish.
abstract: Reactive oxygen species (ROS) are one of the main causes for decreased viability in cryopreserved sperm. Many studies have reported the beneficial effect of antioxidant supplements in freezing media for post-thaw sperm quality. In the present study, we explored two new approaches of ROS inhibition in sperm cryopreservation of yellow catfish, namely mitochondrial-targeted antioxidant and metabolic modulator targeting mitochondrial uncoupling pathways. Our study revealed that addition of MitoQ, a compound designed to deliver ubiquinone into mitochondria, significantly decreased ROS production, as well as lipid peroxidation, and increased post-thaw viability. Similarly, sperm incubated with 2,4-dinitrophenol (DNP), a chemical protonophore that induces mitochondrial uncoupling, also had reduced ROS production, as well as lipid peroxidation, and increased post-thaw sperm viability. Conversely, activation of uncoupling protein (UCP2) by 4-hydroxynonenal (HNE) neither reduced ROS production nor increased post-thaw sperm viability. Our findings indicate that ROS inhibition through mitochondrial-targeted antioxidant or mild mitochondrial uncoupling is beneficial for sperm cryopreservation in yellow catfish. Our study provides novel methods to mitigate oxidative stress induced damage in cryopreserved sperm for future applications.
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Fang
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, PR China.
authors.forename: Chenglian
authors.initials: C
authors.lastname: Bai
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, PR China.
authors.forename: Yuanhong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Dai
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, PR China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Ji
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, PR China. Electronic address: cjhuang5711@163.com.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Huang
authors.affiliation: Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, PR China. Electronic address: dqxdong@163.com.
authors.forename: Qiaoxiang
authors.initials: Q
authors.lastname: Dong
mesh_terms: D019297:2,4-Dinitrophenol / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002397:Catfishes / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D029941:Fish Proteins / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism*; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013094:Spermatozoa / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D000447:Aldehydes; D000975:Antioxidants; D029941:Fish Proteins; D024101:Mitochondrial Proteins; D009943:Organophosphorus Compounds; D017382:Reactive Oxygen Species; D014451:Ubiquinone; C429014:mitoquinone; C027576:4-hydroxy-2-nonenal; D019297:2,4-Dinitrophenol
keywords: 2,4-Dinitrophenol (DNP); MitoQ; Reactive oxygen species (ROS); Sperm cryopreservation; Uncoupling protein (UCP2); Yellow catfish