title: [Effect of dopamine receptor agonist apomorphine on scopolamine induced memory deficits in mice].
abstract: OBJECTIVE
  To research the mechanism of dopamine (DA) controlled memory in mice.
  
  
  METHODS
  Mice received i.p. injection of scopolamine (0.3 mg/kg, SCOP 0.3, and 3.0mg/kg, SCOP 3.0, respectively, n = 10) and saline (NS, n = 10) for 60 days in experiment 1. Memory of mice was detected by dark avoidance behavior in the 53" d and the 60"' d. Animals were sacrificed after the memory test; brain tissues were processed for Fos-ir and TH-ir by immunohistochemistry. Mice were divided into four groups according results of expri-ment 1, they received i.p. injection of apomorphine (0.1 mg/kg, APO 0.1, 0.5 mg/kg, APO 0.5, and 2.0 mg/kg, APO2.0 respectively, n = 10).
  
  
  RESULTS
  Memory was inhibited in mice injected scopolamine 3.0 mg/kg. Latency was significantly less than in NS group, only 1/ 4 that of NS group (P > 0.05). The number of mistake of SCOP 3.0 group increased about four times than that of NS group (P > 0.05). But there was no difference of latency and number of mistake between SCOP 0.3 and NS group in expriment 1. Scopolamine-induced memory deficit was associated with decreased cellular activation, indicated by Fos immunoreactive (ir) staining, in NAcc CA1 and CA3 (P < 0.05), and also associated with decreases in the number of cells labeled for tyrosine hydroxylase (TH-ir), the rate limiting enzyme for dopamine conversion (P < 0.01) and the number of cells co-labeled for TH-ir/Fos-ir (P <0.01) in the ventral tegmental area(VTA), apomorphine lessened scopolamine-induced memory deficit in experiment 2. The number of cells co-labeled for TH-ir/Fos-ir (P <, 0.05) was increased in VTA after apomorphine treatment.
  
  
  CONCLUSIONS
  Apomorphine lessened scopolamine-induced memory deficit in mice by increasing DA activities in VTA.
authors.forename: Hui-Di
authors.initials: HD
authors.lastname: Yang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Yang
authors.forename: Tao-Di
authors.initials: TD
authors.lastname: Liu
mesh_terms: D000818:Animals; D001058:Apomorphine / Q000494:pharmacology*; D004195:Disease Models, Animal; D018491:Dopamine Agonists / Q000494:pharmacology; D008297:Male; D008569:Memory Disorders / Q000139:chemically induced / Q000188:drug therapy*; D051379:Mice; D012601:Scopolamine / Q000633:toxicity*
chemical_list: D018491:Dopamine Agonists; D012601:Scopolamine; D001058:Apomorphine