title: Dynamics of CRISPR loci in microevolutionary process of Yersinia pestis strains.
abstract: The potential use of CRISPR loci genotyping to elucidate population dynamics and microevolution of 146 Yersinia pestis strains from different biovars and locations was investigated in this work. The majority of strains from the Orientalis biovar presented specific spacer arrays, allowing for the establishment of a CRISPR signature for their respective isolates. Twenty-one new spacers were found in the Y. pestis strains from plague foci in Brazil. Ninety-three (64%) strains were grouped in the G1 genotype, whereas the others were distributed in 35 genotypes. This study allowed observing a microevolutionary process in a group of Y. pestis isolated from Brazil. We also identified specific genotypes of Y. pestis that were important for the establishment of the bacteria in plague foci in Brazil. The data have provided supporting evidence for the diversity and dynamics of CRISPR loci present in the genome of Y. pestis strains from plague foci in Brazil.
authors.affiliation: Departamento de Microbiologia, Centro de Pesquisas Aggeu Magalhães (CPqAM/Fiocruz), Recife, PE, Brasil.
authors.forename: Maria Paloma S
authors.initials: MP
authors.lastname: Barros
authors.affiliation: Departamento de Microbiologia, Centro de Pesquisas Aggeu Magalhães (CPqAM/Fiocruz), Recife, PE, Brasil.
authors.forename: Camila T
authors.initials: CT
authors.lastname: França
authors.affiliation: Departamento de Microbiologia, Centro de Pesquisas Aggeu Magalhães (CPqAM/Fiocruz), Recife, PE, Brasil; Departamento de Genética, Universidade Federal de Pernambuco (UFPE), Recife, PE, Brasil.
authors.forename: Rosanny Holanda F B
authors.initials: RH
authors.lastname: Lins
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Pernambuco (UFPE), Recife, PE, Brasil.
authors.forename: Milena Danda V
authors.initials: MD
authors.lastname: Santos
authors.affiliation: Departamento de Microbiologia, Centro de Pesquisas Aggeu Magalhães (CPqAM/Fiocruz), Recife, PE, Brasil.
authors.forename: Ednaldo J
authors.initials: EJ
authors.lastname: Silva
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Pernambuco (UFPE), Recife, PE, Brasil.
authors.forename: Maria Betânia M
authors.initials: MB
authors.lastname: Oliveira
authors.affiliation: Departamento de Biologia, Universidade de Pernambuco (UPE), Garanhuns, PE, Brasil.
authors.forename: Vladimir M
authors.initials: VM
authors.lastname: Silveira-Filho
authors.affiliation: Departamento de Microbiologia, Centro de Pesquisas Aggeu Magalhães (CPqAM/Fiocruz), Recife, PE, Brasil.
authors.forename: Antônio M
authors.initials: AM
authors.lastname: Rezende
authors.affiliation: Departamento de Genética, Universidade Federal de Pernambuco (UFPE), Recife, PE, Brasil.
authors.forename: Valdir Q
authors.initials: VQ
authors.lastname: Balbino
authors.affiliation: Departamento de Microbiologia, Centro de Pesquisas Aggeu Magalhães (CPqAM/Fiocruz), Recife, PE, Brasil.
authors.forename: Tereza Cristina
authors.initials: TC
authors.lastname: Leal-Balbino
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D001483:Base Sequence; D001938:Brazil; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D021901:DNA, Intergenic / Q000235:genetics*; D019143:Evolution, Molecular; D005798:Genes, Bacterial; D014644:Genetic Variation; D016680:Genome, Bacterial; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D010930:Plague / Q000382:microbiology; D017422:Sequence Analysis, DNA; D015010:Yersinia pestis / Q000145:classification / Q000235:genetics*; D015011:Yersinia pseudotuberculosis / Q000235:genetics
chemical_list: D020121:5' Untranslated Regions; D004269:DNA, Bacterial; D021901:DNA, Intergenic