title: Polygenic in vivo validation of cancer mutations using transposons.
abstract: The in vivo validation of cancer mutations and genes identified in cancer genomics is resource-intensive because of the low throughput of animal experiments. We describe a mouse model that allows multiple cancer mutations to be validated in each animal line. Animal lines are generated with multiple candidate cancer mutations using transposons. The candidate cancer genes are tagged and randomly expressed in somatic cells, allowing easy identification of the cancer genes involved in the generated tumours. This system presents a useful, generalised and efficient means for animal validation of cancer genes.
authors.forename: Su Kit
authors.initials: SK
authors.lastname: Chew
authors.forename: Dong
authors.initials: D
authors.lastname: Lu
authors.forename: Lia S
authors.initials: LS
authors.lastname: Campos
authors.forename: Kenneth L
authors.initials: KL
authors.lastname: Scott
authors.forename: Abdel
authors.initials: A
authors.lastname: Saci
authors.forename: Juexuan
authors.initials: J
authors.lastname: Wang
authors.forename: Adam
authors.initials: A
authors.lastname: Collinson
authors.forename: Keiran
authors.initials: K
authors.lastname: Raine
authors.forename: Jonathan
authors.initials: J
authors.lastname: Hinton
authors.forename: Jon W
authors.initials: JW
authors.lastname: Teague
authors.forename: David
authors.initials: D
authors.lastname: Jones
authors.forename: Andrew
authors.initials: A
authors.lastname: Menzies
authors.forename: Adam P
authors.initials: AP
authors.lastname: Butler
authors.forename: John
authors.initials: J
authors.lastname: Gamble
authors.forename: Sarah
authors.initials: S
authors.lastname: O'Meara
authors.forename: Stuart
authors.initials: S
authors.lastname: McLaren
authors.forename: Lynda
authors.initials: L
authors.lastname: Chin
authors.forename: Pentao
authors.initials: P
authors.lastname: Liu
authors.forename: P Andrew
authors.initials: PA
authors.lastname: Futreal
mesh_terms: D000818:Animals; D063646:Carcinogenesis / Q000235:genetics; D002478:Cells, Cultured; D018920:Coculture Techniques; D004251:DNA Transposable Elements; D056726:Genetic Association Studies / Q000379:methods*; D020022:Genetic Predisposition to Disease; D006801:Humans; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008822:Mice, Transgenic; D020412:Multifactorial Inheritance; D009154:Mutation; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements