title: Cloning and characterization of the porcine DBC1 gene encoding deleted in bladder cancer.
abstract: Deleted in bladder cancer 1 (DBC1) is a tumour suppressor which is involved in the regulation of cell growth and programmed cell death. In this study we report the cloning and characterization of porcine DBC1 cDNA. RT-PCR cloning produced a cDNA with an open reading frame of 2,283 bp encoding a polypeptide of 761 amino acids with a predicted molecular mass of 88.6 kDa and estimated isoelectric point of 9.1. The encoded pig DBC1 protein shows a very high amino acid similarity to human (99 %) and to mouse (98 %) DBC1. The porcine DBC1 gene was mapped to chromosome 1. The nucleotide sequence of the promoter displayed a high degree of conservation of elements responsible for neuron-specific expression. The porcine DBC1 gene was found to be highly expressed in brain tissues. The methylation status of the porcine DBC1 gene was examined in brain and liver by bisulfite sequencing. Methylation percentages of 53-61 were observed for the gene body whereas significantly lower values (1-4 %) were found in exon 1 and the promoter sequence of DBC1. The sequences of the porcine DBC1 cDNA and the DBC1 promoter and exon 1 sequence have been submitted to DDBJ/EMBL/GenBank under the accession numbers KF733442 and KJ396193, respectively.
authors.affiliation: Department of Molecular Biology and Genetics, Science and Technology, Aarhus University, Blichers Alle 20, P.O. Box 50, 8830, Tjele, Denmark, Knud.Larsen@agrsci.dk.
authors.forename: Knud
authors.initials: K
authors.lastname: Larsen
authors.forename: Jamal
authors.initials: J
authors.lastname: Momeni
authors.forename: Leila
authors.initials: L
authors.lastname: Farajzadeh
authors.forename: Christian
authors.initials: C
authors.lastname: Bendixen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D019295:Computational Biology; D019175:DNA Methylation; D018076:DNA, Complementary / Q000235:genetics; D019143:Evolution, Molecular; D015870:Gene Expression; D035683:MicroRNAs / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D013552:Swine / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D035683:MicroRNAs; D025521:Tumor Suppressor Proteins