title: [GPI-PLD inhibits the growth of hepatoma cells by down-regulation of PI3K-Akt signaling pathway].
abstract: OBJECTIVE
  To clarify the effect of glycosylphosphatidylinositol-specific phospholipase D (GPIPLD) on hepatoma cells HepG2 and the possible molecular mechanism.
  
  
  METHODS
  MTT, fluorescent staining and Western blot were applied to analyze the effect and molecular mechanism of GPI-PLD on hepatoma cells by transfected high expression GPI-PLD model. We inoculated HepG2 in nude mice models to further clarify the effect of GPI-PLD on hepatoma cells in vivo.
  
  
  RESULTS
  Compared with the control groups, PI3K-Akt signaling pathway activity and proliferation of hepatoma cells were significantly inhibited in the GPI-PLD group. Nude mice models showed that the tumor growth and tumor weight [(1.87 ± 0.09) g] of the GPI-PLD group were significantly less than those of the blank control group [(2.20 ± 0.17) g] and the negative control group [(2.15 ± 0.09) g]. AST, ALT and AFP serum concentration in the GPI-PLD group were significantly lower than those of the control groups (P<0.05).
  
  
  CONCLUSIONS
  GPI-PLD can inhibit the proliferation of hepatoma cells and growth in vivo, and promote the apoptosis of hepatoma cells by reducing the activity of PI3K-Akt signaling pathway.
authors.affiliation: Department of Human Anatomy and Histology,School of Basic Medical Science, Central South University, Changsha 410013; Department of Pharmacy, Changsha Health Vocational College, Changhsha 410100, China.
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Yang
authors.forename: Chaochao
authors.initials: C
authors.lastname: Tan
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Yang
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Tang
mesh_terms: D000818:Animals; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D002460:Cell Line; D015536:Down-Regulation; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D019869:Phosphatidylinositol 3-Kinases; D010739:Phospholipase D / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection
chemical_list: D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D010739:Phospholipase D; C065017:glycoprotein phospholipase D