title: Greater taxol yield of fungus Pestalotiopsis hainanensis from dermatitic scurf of the giant panda (Ailuropoda melanoleuca).
abstract: While taxol yields of fungi from non-animal sources are still low, whether Pestalotiopsis hainanensis isolated from the scurf of a dermatitic giant panda, Ailuropoda melanoleuca, provides a greater taxol yield remains unknown. The objective of the study was to determine the corresponding taxol yield. The structure of the taxol produced by the fungus was evaluated by thin layer chromatography (TLC), ultraviolet (UV) spectroscopy, high-performance liquid chromatography (HPLC), (1)H and (13)C nuclear magnetic resonance spectroscopy ((1)H-NMR and (13)C-NMR), and time-of-flight mass spectrometry (TOF-MS), with standard taxol as a control. The results demonstrated that the P. hainanensis fungus produced taxol, which had the same structure as the standard taxol and yield of 1,466.87 μg/L. This fungal taxol yield from the dermatitic giant panda was significantly greater than those of fungus from non-animal sources. The taxol-producing fungus may be a potential candidate for the production of taxol on an industrial scale.
authors.affiliation: College of Life Sciences, Sichuan Agricultural University, Ya'an, 625014, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Gu
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Ma
authors.forename: Chengdong
authors.initials: C
authors.lastname: Wang
authors.forename: Guizhou
authors.initials: G
authors.lastname: Yue
authors.forename: Yuetian
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yunyan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shanshan
authors.initials: S
authors.lastname: Li
authors.forename: Shanshan
authors.initials: S
authors.lastname: Ling
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Liu
authors.forename: Xintian
authors.initials: X
authors.lastname: Wen
authors.forename: Sanjie
authors.initials: S
authors.lastname: Cao
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Huang
authors.forename: Junliang
authors.initials: J
authors.lastname: Deng
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Zuo
authors.forename: Shumin
authors.initials: S
authors.lastname: Yu
authors.forename: Liuhong
authors.initials: L
authors.lastname: Shen
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
mesh_terms: D000818:Animals; D063807:Dandruff / Q000382:microbiology; D017239:Paclitaxel / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D012867:Skin / Q000382:microbiology; D001503:Ursidae / Q000382:microbiology*; D013098:Xylariales / Q000737:chemistry* / Q000378:metabolism
chemical_list: D017239:Paclitaxel