title: Characterization of acid-and pepsin-soluble collagens from spines and skulls of skipjack tuna (Katsuwonus pelamis).
abstract: Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from the spine (ASC-SP and PSC-SP) and skull (ASC-SK and PSC-SK) of the skipjack tuna, Katsuwonus pelamis, were successfully isolated and characterized. The yields of ASC-SP, PSC-SP, ASC-SK and PSC-SK were (2.47 ± 0.39)%, (5.62 ± 0.82)%, (3.57 ± 0.40)%, and (6.71 ± 0.81)%, respectively, on the basis of dry weight. The four collagens contained Gly (330.2-339.1 residues/1 000 residues) as the major amino acid, and their imino acid contents were between 168.8 and 178.2 residues/1 000 residues. Amino acid composition, SDS-PAGE, and FTIR investigations confirmed that ASC-SP and ASC-SK were mainly composed of type I collagen, and had higher contents of high-molecular weight cross-links than those of PSC-SK and PSC-SP. The FTIR investigation also certified all the collagens had triple helical structure. The denaturation temperatures of ASC-SK, PSC-SK, ASC-SP, and PSC-SP were 17.8, 16.6, 17.6, and 16.5 °C, respectively. All isolated collagens were soluble at acidic pH (1-5) and lost their solubilities when the NaCl concentration was above 2% (W/V). The isolated collagens from the spines and skulls of skipjack tuna could serve as an alternative source of collagens for further application in food, cosmetic, biomedical, and pharmaceutical industries.
authors.affiliation: Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Di
authors.initials: D
authors.lastname: Yu
authors.affiliation: Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Chang-Feng
authors.initials: CF
authors.lastname: Chi
authors.affiliation: Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, China. Electronic address: wangbin4159@hotmail.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, China. Electronic address: dinggf@zjou.edu.cn.
authors.forename: Guo-Fang
authors.initials: GF
authors.lastname: Ding
authors.affiliation: Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Zhong-Rui
authors.initials: ZR
authors.lastname: Li
mesh_terms: D000143:Acids / Q000737:chemistry; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003094:Collagen / Q000737:chemistry / Q000302:isolation & purification*; D024042:Collagen Type I / Q000737:chemistry / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D008970:Molecular Weight; D010434:Pepsin A / Q000737:chemistry; D012886:Skull / Q000737:chemistry*; D012965:Sodium Chloride; D012995:Solubility; D013131:Spine / Q000737:chemistry*; D013696:Temperature; D014413:Tuna
chemical_list: D000143:Acids; D000596:Amino Acids; D024042:Collagen Type I; D012965:Sodium Chloride; D003094:Collagen; D010434:Pepsin A
keywords: Acid soluble collagen (ASC); Characterization; Katsuwonus pelamis; Pepsin soluble collagen (PSC); Skipjack tuna; Skull; Spine