title: Proteomics analysis for finding serum markers of ovarian cancer.
abstract: A combination of peptide ligand library beads (PLLB) and 1D gel liquid chromatography-mass spectrometry/mass spectrometry (1DGel-LC-MS/MS) was employed to analyze serum samples from patients with ovarian cancer and from healthy controls. Proteomic analysis identified 1200 serum proteins, among which 57 proteins were upregulated and 10 were downregulated in the sera from cancer patients. Retinol binding protein 4 (RBP4) is highly upregulated in the ovarian cancer serum samples. ELISA was employed to measure plasma concentrations of RBP4 in 80 samples from ovarian cancer patients, healthy individuals, myoma patients, and patients with benign ovarian tumor, respectively. The plasma concentrations of RBP4 ranging from 76.91 to 120.08 ng/mL with the mean value 89.13 ± 1.67 ng/mL in ovarian cancer patients are significantly higher than those in healthy individuals (10.85 ± 2.38 ng/mL). Results were further confirmed with immunohistochemistry, demonstrating that RBP4 expression levels in normal ovarian tissue were lower than those in ovarian cancer tissues. Our results suggested that RBP4 is a potential biomarker for diagnostic of screening ovarian cancer.
authors.affiliation: Beijing Chaoyang Hospital Affiliated with Capital Medical University, Beijing 100020, China.
authors.forename: Yushan
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Beijing Chaoyang Hospital Affiliated with Capital Medical University, Beijing 100020, China.
authors.forename: Chongdong
authors.initials: C
authors.lastname: Liu
authors.affiliation: Beijing Chaoyang Hospital Affiliated with Capital Medical University, Beijing 100020, China.
authors.forename: Nawei
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Shengdian
authors.initials: S
authors.lastname: Wang
authors.affiliation: Beijing Chaoyang Hospital Affiliated with Capital Medical University, Beijing 100020, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood*; D005260:Female; D006801:Humans; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood*; D010051:Ovarian Neoplasms / Q000097:blood* / Q000175:diagnosis*; D019151:Peptide Library; D010449:Peptide Mapping / Q000379:methods; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D054839:Retinol-Binding Proteins, Plasma / Q000235:genetics*; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D019151:Peptide Library; D020543:Proteome; C101326:RBP4 protein, human; D054839:Retinol-Binding Proteins, Plasma