title: Development of a multiplex PCR assay for identification of Klebsiella pneumoniae hypervirulent clones of capsular serotype K2.
abstract: Hypervirulent Klebsiella pneumoniae isolates of capsular serotype K2 (hvKP-K2) that cause community-acquired invasive infections represent several unrelated clones, which all belong to phylogenetic group KpI. These clones can be recognized using multilocus sequence typing and genomic analyses, but no rapid method currently exists to differentiate them. In this work, a multiplex PCR assay was developed to identify three hvKP-K2 groups: (i) sequence type (ST)86; (ii) ST380 and ST679 (i.e. clonal group 380); and (iii) ST65 and ST375. A specific genetic marker, Kp50233, allowing K. pneumoniae sensu stricto (corresponding to phylogroup KpI) to be distinguished from closely related species, was included in the assay. This PCR assay will be useful in better defining the epidemiology and clinical features of emerging virulent K. pneumoniae clones.
authors.affiliation: Service de Microbiologie, Hôpital Beaujon, AP-HP, 100 boulevard du Général Leclerc, 92110 Clichy, France.|CNRS, UMR3525, Paris, France.|Institut Pasteur, Microbial Evolutionary Genomics, 28 rue du Dr Roux, 75724 Paris, France.
authors.forename: Suzanne
authors.initials: S
authors.lastname: Bialek-Davenet
authors.affiliation: CNRS, UMR3525, Paris, France.|Institut Pasteur, Microbial Evolutionary Genomics, 28 rue du Dr Roux, 75724 Paris, France.
authors.forename: Alexis
authors.initials: A
authors.lastname: Criscuolo
authors.affiliation: CNRS, UMR3525, Paris, France.|Institut Pasteur, Microbial Evolutionary Genomics, 28 rue du Dr Roux, 75724 Paris, France.
authors.forename: Florent
authors.initials: F
authors.lastname: Ailloud
authors.affiliation: CNRS, UMR3525, Paris, France.|Institut Pasteur, Microbial Evolutionary Genomics, 28 rue du Dr Roux, 75724 Paris, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Passet
authors.affiliation: INSERM UMR 1149, Université Paris Diderot - Paris 7, Paris, France.|Faculté de Médecine, Université Paris Diderot - Paris 7, Paris, France.|Service de Microbiologie, Hôpital Beaujon, AP-HP, 100 boulevard du Général Leclerc, 92110 Clichy, France.
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Nicolas-Chanoine
authors.affiliation: INSERM U1135, Centre d'Immunologie et des Maladies Infectieuses, CIMI, team E13 (Bacteriology), 75013 Paris, France.|Sorbonne Universités, UPMC Univ Paris 06 CR7, Centre d'Immunologie et des Maladies Infectieuses, CIMI, team E13 (Bacteriology), 75013.|Laboratoire de Bactériologie, Hôpital Saint-Antoine, AP-HP, 184 rue du Faubourg Saint-Antoine, 75012 Paris, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Decré
authors.affiliation: CNRS, UMR3525, Paris, France.|Institut Pasteur, Microbial Evolutionary Genomics, 28 rue du Dr Roux, 75724 Paris, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Brisse
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D005838:Genotype; D006801:Humans; D007710:Klebsiella Infections / Q000175:diagnosis* / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D065288:Serogroup