title: Effect of the French oak wood extract Robuvit on markers of oxidative stress and activity of antioxidant enzymes in healthy volunteers: a pilot study.
abstract: We examined in vitro antioxidant capacity of polyphenolic extract obtained from the wood of oak Quercus robur (QR), Robuvit, using TEAC (Trolox equivalent antioxidant capacity) method and the effect of its intake on markers of oxidative stress, activity of antioxidant enzymes, and total antioxidant capacity in plasma of 20 healthy volunteers. Markers of oxidative damage to proteins, DNA, and lipids and activities of Cu/Zn-superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were determined in the erythrocytes. We have found an in vitro antioxidant capacity of Robuvit of 6.37 micromole Trolox equivalent/mg of Robuvit. One month intake of Robuvit in daily dose of 300 mg has significantly decreased the serum level of advanced oxidation protein products (AOPP) and lipid peroxides (LP). Significantly increased activities of SOD and CAT as well as total antioxidant capacity of plasma after one month intake of Robuvit have been shown. In conclusion, we have demonstrated for the first time that the intake of Robuvit is associated with decrease of markers of oxidative stress and increase of activity of antioxidant enzymes and total antioxidant capacity of plasma in vivo.
authors.affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, 811 08 Bratislava, Slovakia.
authors.forename: Martina
authors.identifier: 0000-0002-8610-2061
authors.initials: M
authors.lastname: Horvathova
authors.affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, 811 08 Bratislava, Slovakia.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Orszaghova
authors.affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, 811 08 Bratislava, Slovakia ; Institute of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius University, Malá Hora 4, 036 01 Martin, Slovakia.
authors.forename: Lucia
authors.initials: L
authors.lastname: Laubertova
authors.affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, 811 08 Bratislava, Slovakia.
authors.forename: Magdalena
authors.identifier: 0000-0003-1999-1388
authors.initials: M
authors.lastname: Vavakova
authors.affiliation: 2nd Department of Internal Medicine, Faculty of Medicine, Comenius University, Mickiewiczova 13, 813 69 Bratislava, Slovakia.
authors.forename: Peter
authors.initials: P
authors.lastname: Sabaka
authors.affiliation: Westfälische Wilhelms-Universität Münster, Institut für Pharmazeutische Chemie, Twenteweg 15, 48161 Münster, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Rohdewald
authors.affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, 811 08 Bratislava, Slovakia.
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Durackova
authors.affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, 811 08 Bratislava, Slovakia.
authors.forename: Jana
authors.initials: J
authors.lastname: Muchova
mesh_terms: D000368:Aged; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D064368:Healthy Volunteers; D006801:Humans; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D010865:Pilot Projects; D010936:Plant Extracts / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D047348:Hydrolyzable Tannins; D010936:Plant Extracts; C588251:Robuvit; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase