title: [Determination of urinary cotinine of children exposed to passive smoking by stable isotope dilution gas chromatography-triple quadrupole mass spectrometry].
abstract: An analytical method for the determination of urinary cotinine of children exposed to passive smoking was established based on stable isotope dilution by gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS). The samples were extracted and purified with chloroform. The extracts were determined by GC-MS/MS in multiple reaction monitoring (MRM) mode. The cotinine-d3 as an isotope internal standard was applied to quantify and confirm the urinary cotinine of children exposed to passive smoking. The method had a good linearity from 0.1 microg/L to 10 microg/L with the correlation coefficient (r) > 0.998. The recoveries of the cotinine in blank urine were from 79.2% to 112.8% at spiked levels of 0.1, 1.0 and 10 microg/ L, with relative standard deviations (RSDs) from 2.1% to 5. 8%. The limit of quantification ( LOQ) of the method was 0.1 microg/L. The developed method is accurate, sensitive, rapid and can be applied to detect urinary cotinine of children exposed to passive smoking at home.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Ye
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shuiyuan
authors.initials: S
authors.lastname: Xiao
mesh_terms: D002648:Child; D003367:Cotinine / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007554:Isotopes; D053719:Tandem Mass Spectrometry; D014028:Tobacco Smoke Pollution
chemical_list: D007554:Isotopes; D014028:Tobacco Smoke Pollution; D003367:Cotinine