title: Combining experimental evolution with next-generation sequencing: a powerful tool to study adaptation from standing genetic variation.
abstract: Evolve and resequence (E&R) is a new approach to investigate the genomic responses to selection during experimental evolution. By using whole genome sequencing of pools of individuals (Pool-Seq), this method can identify selected variants in controlled and replicable experimental settings. Reviewing the current state of the field, we show that E&R can be powerful enough to identify causative genes and possibly even single-nucleotide polymorphisms. We also discuss how the experimental design and the complexity of the trait could result in a large number of false positive candidates. We suggest experimental and analytical strategies to maximize the power of E&R to uncover the genotype-phenotype link and serve as an important research tool for a broad range of evolutionary questions.
authors.affiliation: Institut für Populationsgenetik, Vetmeduni Vienna, Vienna, Austria.
authors.forename: C
authors.initials: C
authors.lastname: Schlötterer
authors.affiliation: Institut für Populationsgenetik, Vetmeduni Vienna, Vienna, Austria.
authors.forename: R
authors.initials: R
authors.lastname: Kofler
authors.affiliation: 1] Institut für Populationsgenetik, Vetmeduni Vienna, Vienna, Austria [2] Center for Mind/Brain Sciences, University of Trento, Rovereto, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Versace
authors.affiliation: 1] Institut für Populationsgenetik, Vetmeduni Vienna, Vienna, Austria [2] Vienna Graduate School of Population Genetics, Vienna, Austria.
authors.forename: R
authors.identifier: 0000000246031473
authors.initials: R
authors.lastname: Tobler
authors.affiliation: Institut für Populationsgenetik, Vetmeduni Vienna, Vienna, Austria.
authors.forename: S U
authors.initials: SU
authors.lastname: Franssen
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000818:Animals; D005075:Biological Evolution; D004331:Drosophila melanogaster / Q000235:genetics; D005787:Gene Frequency; D056726:Genetic Association Studies; D014644:Genetic Variation; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D012107:Research Design