title: Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II.
abstract: In mitosis, the Greatwall kinase (called microtubule-associated serine/threonine kinase like [Mastl] in mammals) is essential for prometaphase entry or progression by suppressing protein phosphatase 2A (PP2A) activity. PP2A suppression in turn leads to high levels of Cdk1 substrate phosphorylation. We have used a mouse model with an oocyte-specific deletion of Mastl to show that Mastl-null oocytes resume meiosis I and reach metaphase I normally but that the onset and completion of anaphase I are delayed. Moreover, after the completion of meiosis I, Mastl-null oocytes failed to enter meiosis II (MII) because they reassembled a nuclear structure containing decondensed chromatin. Our results show that Mastl is required for the timely activation of anaphase-promoting complex/cyclosome to allow meiosis I exit and for the rapid rise of Cdk1 activity that is needed for the entry into MII in mouse oocytes.
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, S-405 30 Gothenburg, Sweden.
authors.forename: Deepak
authors.initials: D
authors.lastname: Adhikari
authors.affiliation: Institute of Molecular and Cell Biology, A*STAR, Singapore 138673, Republic of Singapore.
authors.forename: M Kasim
authors.initials: MK
authors.lastname: Diril
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, S-405 30 Gothenburg, Sweden.
authors.forename: Kiran
authors.initials: K
authors.lastname: Busayavalasa
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, S-405 30 Gothenburg, Sweden.
authors.forename: Sanjiv
authors.initials: S
authors.lastname: Risal
authors.affiliation: Institute of Reproductive and Developmental Biology, Department of Surgery and Cancer, Hammersmith Hospital, Imperial College London, London W12 0NN, England, UK.
authors.forename: Shoma
authors.initials: S
authors.lastname: Nakagawa
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, S-405 30 Gothenburg, Sweden.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Lindkvist
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, S-405 30 Gothenburg, Sweden.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.affiliation: National Cancer Institute, Mouse Cancer Genetics Program, National Cancer Institute-Frederick, Frederick, MD 21702.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Coppola
authors.affiliation: National Cancer Institute, Mouse Cancer Genetics Program, National Cancer Institute-Frederick, Frederick, MD 21702.
authors.forename: Lino
authors.initials: L
authors.lastname: Tessarollo
authors.affiliation: Institute of Reproductive and Developmental Biology, Department of Surgery and Cancer, Hammersmith Hospital, Imperial College London, London W12 0NN, England, UK.
authors.forename: Nobuaki R
authors.initials: NR
authors.lastname: Kudo
authors.affiliation: Institute of Molecular and Cell Biology, A*STAR, Singapore 138673, Republic of Singapore Department of Biochemistry, National University of Singapore, Singapore 117599, Republic of Singapore kaldis@imcb.a-star.edu.sg kui.liu@gu.se.
authors.forename: Philipp
authors.initials: P
authors.lastname: Kaldis
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, S-405 30 Gothenburg, Sweden kaldis@imcb.a-star.edu.sg kui.liu@gu.se.
authors.forename: Kui
authors.initials: K
authors.lastname: Liu
mesh_terms: D000705:Anaphase; D064173:Anaphase-Promoting Complex-Cyclosome / Q000378:metabolism*; D000818:Animals; D016203:CDC2 Protein Kinase / Q000235:genetics / Q000378:metabolism*; D004790:Enzyme Induction; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D008540:Meiosis; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000502:physiology*; D009865:Oocytes / Q000201:enzymology; D010766:Phosphorylation; D054648:Protein Phosphatase 2 / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D017346:Protein Serine-Threonine Kinases / Q000502:physiology*; D059010:Single-Cell Analysis
chemical_list: D008869:Microtubule-Associated Proteins; D064173:Anaphase-Promoting Complex-Cyclosome; D017346:Protein Serine-Threonine Kinases; C585164:greatwall protein, mouse; D016203:CDC2 Protein Kinase; D054648:Protein Phosphatase 2