title: Quantitative analysis of elastase and cathepsin G mRNA levels in peripheral blood CD14(+) cells from patients with rheumatoid arthritis.
abstract: In rheumatoid arthritis (RA) activity of serine proteases is an important factor contributing to destructive changes in the joints. The aim of this study was to compare elastase (ELANE) and cathepsin G (CTSG) mRNA levels in peripheral blood CD14(+) cells obtained from RA patients, healthy subjects (HS) and patients with osteoarthritis (OA). CD14(+) cells were isolated from peripheral blood by positive magnetic selection. The expression levels of ELANE and CTSG were determined by quantitative real-time PCR. ELANE mRNA expression was significantly higher in RA patients when compared to HS (p<0.001) and OA patients (p<0.001). The results suggest that in RA, peripheral blood CD14(+) cells express serine protease mRNA as a result of systemic mechanisms probably related to inflammation/cytokines before entering inflamed joints.
authors.affiliation: Department of Rheumatology and Clinical Immunology, Poznan University of Medical Sciences, Przybyszewskiego Street 39, 60-356 Poznan, Poland. Electronic address: dtrzybulska@wp.pl.
authors.forename: Dorota
authors.initials: D
authors.lastname: Trzybulska
authors.affiliation: Department of Rheumatology and Clinical Immunology, Poznan University of Medical Sciences, Przybyszewskiego Street 39, 60-356 Poznan, Poland. Electronic address: anolegaw@wp.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Olewicz-Gawlik
authors.affiliation: Department of Rheumatology and Clinical Immunology, Poznan University of Medical Sciences, Przybyszewskiego Street 39, 60-356 Poznan, Poland. Electronic address: kasiagraniczna@wp.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Graniczna
authors.affiliation: Department of Rheumatology, Koscian Municipal Hospital, Szpitalna Street 7, 64-000 Koscian, Poland. Electronic address: kajetan.kisiel@interia.pl.
authors.forename: Kajetan
authors.initials: K
authors.lastname: Kisiel
authors.affiliation: Department of Rheumatology, Koscian Municipal Hospital, Szpitalna Street 7, 64-000 Koscian, Poland. Electronic address: michalmoskal79@gmail.com.
authors.forename: Michał
authors.initials: M
authors.lastname: Moskal
authors.forename: Dorota
authors.initials: D
authors.lastname: Cieślak
authors.forename: Jan
authors.initials: J
authors.lastname: Sikora
authors.affiliation: Department of Rheumatology and Clinical Immunology, Poznan University of Medical Sciences, Przybyszewskiego Street 39, 60-356 Poznan, Poland; Department of Rheumatology, Koscian Municipal Hospital, Szpitalna Street 7, 64-000 Koscian, Poland. Electronic address: pawel.hrycaj@gmail.com.
authors.forename: Paweł
authors.initials: P
authors.lastname: Hrycaj
mesh_terms: D000328:Adult; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D056649:Cathepsin G / Q000276:immunology*; D005260:Female; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology*; D010196:Pancreatic Elastase / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis*
chemical_list: D018950:Lipopolysaccharide Receptors; D012333:RNA, Messenger; D056649:Cathepsin G; D010196:Pancreatic Elastase
keywords: CD14(+) cells; Cathepsin G; Elastase; Monocytes/macrophages; Rheumatoid arthritis