title: Genetic diversity of Echinococcus granulosus in center of Iran.
abstract: Hydatid cyst caused by Echinococcus granulosus is one of the most important parasitic diseases around the world and many countries in Asia, including Iran, are involved with this infection. This disease can cause high mortality in humans as well as economic losses in livestock. To date, several molecular methods have been used to determine the genetic diversity of E. granulosus. So far, identification of E. granulosus using real-time PCR fluorescence-based quantitative assays has not been studied worldwide, also in Iran. Therefore, the aim of this study was to investigate the genetic diversity of E. granulosus from center of Iran using real-time PCR method. A total of 71 hydatid cysts were collected from infected sheep, goat, and cattle slaughtered in Isfahan, Iran during 2013. DNA was extracted from protoscolices and/or germinal layers from each individual cyst and used as template to amplify the mitochondrial cytochrome c oxidase subunit 1 gene (cox1) (420 bp). Five cattle isolates out of 71 isolates were sterile and excluded from further investigation. Overall, of 66 isolates, partial sequences of the cox1 gene of E. granulosus indicated the presence of genotypes G1 in 49 isolates (74.2%), G3 in 15 isolates (22.7%), and G6 in 2 isolates (3.0%) in infected intermediate hosts. Sixteen sequences of G1 genotype had microgenetic variants, and they were compared to the original sequence of cox1. However, isolates identified as G3 and G6 genotypes were completely consistent with original sequences. G1 genotype in livestock was the dominant genotype in Isfahan region, Iran.
authors.affiliation: Department of Medical Parasitology and Mycology Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Nader
authors.initials: N
authors.lastname: Pestechian
authors.affiliation: Department of Medical Parasitology and Mycology Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Hosseini Safa
authors.affiliation: Department of Clinical Biochemistry, School of Pharmacy and Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. ; Physiology Research Center, Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Mohammadhasan
authors.initials: M
authors.lastname: Tajedini
authors.affiliation: Gastroenterology and Liver Disease Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Rostami-Nejad
authors.affiliation: Department of Medical Parasitology and Mycology Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Mousavi
authors.affiliation: Department of Medical Parasitology and Mycology Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Hosseinali
authors.initials: H
authors.lastname: Yousofi
authors.affiliation: Physiology Research Center, Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Shaghayegh
authors.initials: S
authors.lastname: Haghjooy Javanmard
mesh_terms: D000818:Animals; D002417:Cattle; D016000:Cluster Analysis; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004443:Echinococcosis / Q000469:parasitology / Q000662:veterinary*; D048209:Echinococcus granulosus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D003576:Electron Transport Complex IV / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006041:Goats; D007492:Iran; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012756:Sheep
chemical_list: D018509:DNA, Helminth; D003576:Electron Transport Complex IV
keywords: Echinococcus granulosus; cox1 gene; genotyping