title: Commensal bacteria directly suppress in vitro degranulation of mast cells in a MyD88-independent manner.
abstract: The intestine harbors a substantial number of commensal bacteria that provide considerable benefits to the host. Epidemiologic studies have identified associations between alterations in the composition of the intestinal microbiota and the development of allergic disease. However, the cellular and molecular mechanisms underlying these effects remain to be determined. Here, we show that heat-killed commensal bacteria suppressed degranulation of mast cells in vitro in a MyD88-independent manner. In particular, Enterococcus faecalis showed the strongest suppression of degranulation through partial inhibition of Ca(2+) signaling upon the high affinity IgE receptor (FcεRI) cross-linking.
authors.affiliation: a College of Bioresource Sciences , Nihon University , Fujisawa , Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Kasakura
authors.forename: Kyoko
authors.initials: K
authors.lastname: Takahashi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Itoh
authors.forename: Akira
authors.initials: A
authors.lastname: Hosono
authors.forename: Yoshika
authors.initials: Y
authors.lastname: Momose
authors.forename: Kikuji
authors.initials: K
authors.lastname: Itoh
authors.forename: Chiharu
authors.initials: C
authors.lastname: Nishiyama
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kaminogawa
mesh_terms: D000818:Animals; D015550:Cell Degranulation; D013293:Enterococcus faecalis / Q000502:physiology*; D005260:Female; D042541:Intracellular Space / Q000378:metabolism; D008407:Mast Cells / Q000166:cytology*; D051379:Mice; D053594:Myeloid Differentiation Factor 88 / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D053594:Myeloid Differentiation Factor 88
keywords: allergy; commensal bacteria; degranulation; mast cells