title: Identification of the responsible proteins for increased selenium bioavailability in the brain of transgenic rats overexpressing selenoprotein M.
abstract: The present study was conducted to investigate whether the high antioxidant activity induced by selenium (Sel) treatment and selenoprotein M (SelM) overexpression affected the protein profile of the brain cortex. To accomplish this, the changes in global protein expression were measured in transgenic (Tg) rats expressing human SelM (CMV/hSelM) and non‑Tg rats using two-dimensional electrophoresis (2-DE). The results revealed that: ⅰ) CMV/hSelM Tg rats showed a high level of enzyme activity for antioxidant protein in the brain cortex compared to non-Tg rats; ⅱ) the high activity of these enzymes induced a decrease in total antioxidant concentration and γ-secretase activity in CMV/hSelM Tg rats; ⅲ) five proteins were upregulated and three were downregulated by SelM overexpression; ⅳ) among the five upregulated proteins, two associated with creatine kinase B-type (B-CK) and E3 ubiquitin-protein ligase RING1 (RING finger protein 1) were further increased in the two groups following Sel treatment, whereas synaptotagmin-15 (SytXV), eukaryotic translation initiation factor 4H (eIF-4H) and lactate dehydrogenase B (LDH-B) were increased or decreased under the same conditions; ⅴ) the three downregulated proteins did not induce a significant change in expression following Sel treatment; and ⅵ) the protein expression level alterations of the two selected spots (B-CK and SytXV) identified by 2-DE were extremely similar to the results from western blot analysis. Overall, the results of the present study provide primary novel biological evidence that new functional protein groups and individual proteins in the brain cortex of CMV/hSelM Tg rats are associated with Sel biology, including the response to Sel treatment and SelM overexpression.
authors.affiliation: Laboratory of Developmental Biology and Genomics, College of Veterinary Medicine, BK21 Program for Veterinary Science, Interdisciplinary Program for Bioinformatics and Program for Cancer Biology, Seoul National University, Seoul 151‑742, Republic of Korea.
authors.forename: Yona
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Biomaterials Science, College of Natural Resources and Life Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Gyeongsangnam-do 627‑706, Republic of Korea.
authors.forename: Jun Seo
authors.initials: JS
authors.lastname: Goo
authors.affiliation: Laboratory of Developmental Biology and Genomics, College of Veterinary Medicine, BK21 Program for Veterinary Science, Interdisciplinary Program for Bioinformatics and Program for Cancer Biology, Seoul National University, Seoul 151‑742, Republic of Korea.
authors.forename: Il Yong
authors.initials: IY
authors.lastname: Kim
authors.affiliation: Department of Biomaterials Science, College of Natural Resources and Life Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Gyeongsangnam-do 627‑706, Republic of Korea.
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Kim
authors.affiliation: Department of Biomaterials Science, College of Natural Resources and Life Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Gyeongsangnam-do 627‑706, Republic of Korea.
authors.forename: Moon Hwa
authors.initials: MH
authors.lastname: Kwak
authors.affiliation: Department of Biomaterials Science, College of Natural Resources and Life Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Gyeongsangnam-do 627‑706, Republic of Korea.
authors.forename: Jun
authors.initials: J
authors.lastname: Go
authors.affiliation: Department of Laboratory Animal Resources, National Institute of Food and Drug Safety Evaluation, Korea FDA, Cheongwon, Chungcheongbuk-do 363‑700, Republic of Korea.
authors.forename: Sunbo
authors.initials: S
authors.lastname: Shim
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungcheongbuk-do 361‑763, Republic of Korea.
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.affiliation: Department of Biomaterials Science, College of Natural Resources and Life Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Gyeongsangnam-do 627‑706, Republic of Korea.
authors.forename: Dae Youn
authors.initials: DY
authors.lastname: Hwang
authors.affiliation: Laboratory of Developmental Biology and Genomics, College of Veterinary Medicine, BK21 Program for Veterinary Science, Interdisciplinary Program for Bioinformatics and Program for Cancer Biology, Seoul National University, Seoul 151‑742, Republic of Korea.
authors.forename: Je Kyung
authors.initials: JK
authors.lastname: Seong
mesh_terms: D053829:Amyloid Precursor Protein Secretases / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001682:Biological Availability; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D055647:Rats, Transgenic; D012643:Selenium / Q000008:administration & dosage / Q000493:pharmacokinetics*; D051140:Selenoproteins / Q000235:genetics / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D014131:Trace Elements / Q000008:administration & dosage / Q000493:pharmacokinetics; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism
chemical_list: D000975:Antioxidants; D020543:Proteome; C448999:SELM protein, human; D051140:Selenoproteins; D014131:Trace Elements; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D044767:Ubiquitin-Protein Ligases; D053829:Amyloid Precursor Protein Secretases; D012643:Selenium