title: Silver, zinc oxide and titanium dioxide nanoparticle ecotoxicity to bioluminescent Pseudomonas putida in laboratory medium and artificial wastewater.
abstract: Bacteria based ecotoxicology assessment of manufactured nanoparticles is largely restricted to Escherichia coli bioreporters in laboratory media. Here, toxicity effects of model OECD nanoparticles (Ag NM-300K, ZnO NM-110 and TiO2 NM-104) were assessed using the switch-off luminescent Pseudomonas putida BS566::luxCDABE bioreporter in Luria Bertani (LB) medium and artificial wastewater (AW). IC50 values ∼4 mg L(-1), 100 mg L(-1) and >200 mg L(-1) at 1 h were observed in LB for Ag NM-300K, ZnO NM-110 and TiO2 NM-104, respectively. Similar results were obtained in AW for Ag NM-300K (IC50∼5 mg L(-1)) and TiO2 NM-104 (IC50>200 mg L(-1)) whereas ZnO NM-110 was significantly higher (IC50>200 mg L(-1)). Lower ZnO NM-110 toxicity in AW compared to LB was associated with differences in agglomeration status and dissolution rate. This work demonstrates the importance of nanoecotoxicological studies in environmentally relevant matrices.
authors.affiliation: School of Life Sciences, NanoSafety Research Group, Heriot-Watt University, Edinburgh EH14 4AS, Scotland, UK.
authors.forename: Florian
authors.initials: F
authors.lastname: Mallevre
authors.affiliation: School of Life Sciences, NanoSafety Research Group, Heriot-Watt University, Edinburgh EH14 4AS, Scotland, UK.
authors.forename: Teresa F
authors.initials: TF
authors.lastname: Fernandes
authors.affiliation: School of Life Sciences, NanoSafety Research Group, Heriot-Watt University, Edinburgh EH14 4AS, Scotland, UK. Electronic address: T.J.Aspray@hw.ac.uk.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Aspray
mesh_terms: D054750:Ecotoxicology; D004926:Escherichia coli / Q000187:drug effects; D053768:Metal Nanoparticles; D053758:Nanoparticles / Q000633:toxicity*; D016958:Pseudomonas putida / Q000187:drug effects*; D012834:Silver / Q000633:toxicity*; D014025:Titanium / Q000633:toxicity*; D062065:Wastewater / Q000737:chemistry / Q000382:microbiology; D015034:Zinc Oxide / Q000633:toxicity*
chemical_list: D012834:Silver; D014025:Titanium; D062065:Wastewater; D015034:Zinc Oxide; C009495:titanium dioxide
keywords: Bacterial biosensor; Bioreporter; Ecotoxicity; Nanoparticle; Pseudomonas putida; Wastewater