title: Human cytomegalovirus replication supported by virus-induced activation of CCL2-CCR2 interactions.
abstract: We previously revealed that human cytomegalovirus (HCMV) infection can cause aberrant expression of the chemokine IL-8/CXCL8. We first examined the effects of HCMV infection on the expression of another chemokine, CCL2. HCMV infection induced CCL2 expression at the mRNA and protein levels in human embryonic lung fibroblasts cells (HEL). Moreover, HCMV induced the mRNA expression of CCR2, a specific receptor for CCL2. CCL2 siRNA treatment reduced HCMV virion production, and this reduction was reversed by the addition of CCL2. We further observed that CCL2 siRNA, but not control siRNA, reduced the expression of HCMV immediate early gene (IE1) and HCMV UL54 gene (DNA polymerase) in a dose-dependent manner. Thus, HCMV infection is able to activate the CCL2-CCR2 interactions to further enhance HCMV infection and/or replication.
authors.affiliation: Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences, Hokuriku University, Ho-3 Kanagawa-machi, Kanazawa 920-1181, Japan. Electronic address: t-murayama@hokuriku-u.ac.jp.
authors.forename: Tsugiya
authors.initials: T
authors.lastname: Murayama
authors.affiliation: Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences, Hokuriku University, Ho-3 Kanagawa-machi, Kanazawa 920-1181, Japan.
authors.forename: Marie
authors.initials: M
authors.lastname: Kikuchi
authors.affiliation: Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences, Hokuriku University, Ho-3 Kanagawa-machi, Kanazawa 920-1181, Japan.
authors.forename: Takaki
authors.initials: T
authors.lastname: Miita
authors.affiliation: Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences, Hokuriku University, Ho-3 Kanagawa-machi, Kanazawa 920-1181, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Yamada
authors.affiliation: Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences, Hokuriku University, Ho-3 Kanagawa-machi, Kanazawa 920-1181, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Matsubara
authors.affiliation: Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences, Hokuriku University, Ho-3 Kanagawa-machi, Kanazawa 920-1181, Japan.
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Sadanari
authors.affiliation: Division of Molecular Bioregulation, Cancer Research Institute, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Naofumi
authors.initials: N
authors.lastname: Mukaida
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000502:physiology*; D003587:Cytomegalovirus / Q000502:physiology*; D017931:DNA Primers; D006801:Humans; D016133:Polymerase Chain Reaction; D054390:Receptors, CCR2 / Q000502:physiology*; D014779:Virus Replication / Q000502:physiology*
chemical_list: C111835:CCL2 protein, human; C515334:CCR2 protein, human; D018932:Chemokine CCL2; D017931:DNA Primers; D054390:Receptors, CCR2
keywords: CCL2; CCR2; Chemokine; Cytomegalovirus replication; siRNA