title: Enhancement of RNA annealing and strand displacement found in archaeal ribonuclease P proteins is conserved in Escherichia coli protein C5 and yeast protein Rpr2.
abstract: We analyzed modes of action of ribonuclease P (RNase P) proteins, C5 in Escherichia coli and Rpr2 in Saccharomyces cerevisiae, using a pair of complementary fluorescence-labeled oligoribonucleotides. Fluorescence resonance energy transfer-based assays revealed that RNA annealing and strand displacement activities found in archaeal RNase P proteins are prevalent in eubacterial (C5) and eukaryotic (Rpr2) RNase P proteins.
authors.affiliation: a Laboratory of Biochemistry, Faculty of Agriculture, Department of Bioscience and Biotechnology, Graduate School , Kyushu University , Fukuoka , Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Furutani
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Hazeyama
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Ueda
authors.forename: Satoko
authors.initials: S
authors.lastname: Tomita
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Imai
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakashima
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Kakuta
authors.forename: Makoto
authors.initials: M
authors.lastname: Kimura
mesh_terms: D000595:Amino Acid Sequence; D029968:Escherichia coli Proteins / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D043902:Pyrococcus horikoshii / Q000201:enzymology*; D012313:RNA / Q000378:metabolism*; D043262:Ribonuclease P / Q000737:chemistry / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D029701:Saccharomyces cerevisiae Proteins; D012313:RNA; D043262:Ribonuclease P; C000598429:Rpr2 protein, S cerevisiae; C470865:ribonuclease P, E coli
keywords: Pyrococcus horikoshii; RNA chaperone; RNA-binding protein; RNase P proteins; fluorescence resonance energy transfer