title: Potential use of avocado oil on structured lipids MLM-type production catalysed by commercial immobilised lipases.
abstract: Structured Lipids are generally constituents of functional foods. Growing demands for SL are based on a fuller understanding of nutritional requirements, lipid metabolism, and improved methods to produce them. Specifically, this work was aimed to add value to avocado oil by producing dietary triacylglycerols (TAG) containing medium-chain fatty acids (M) at positions sn-1,3 and long-chain fatty acids (L) at position sn-2. These MLM-type structured lipids (SL) were produced by interesterification of caprylic acid (CA) (C8:0) and avocado oil (content of C18:1). The regiospecific sn-1,3 commercial lipases Lipozyme RM IM and TL IM were used as biocatalysts to probe the potential of avocado oil to produce SL. Reactions were performed at 30-50°C for 24 h in solvent-free media with a substrate molar ratio of 1∶2 (TAG:CA) and 4-10% w/w enzyme content. The lowest incorporation of CA (1.1% mol) resulted from Lipozyme RM IM that was incubated at 50°C. The maximum incorporation of CA into sn-1,3 positions of TAG was 29.2% mol. This result was obtained at 30°C with 10% w/w Lipozyme TL IM, which is the highest values obtained in solvent-free medium until now for structured lipids of low-calories. This strategy opens a new market to added value products based on avocado oil.
authors.affiliation: Centro Regional de Estudios en Alimentos Saludables (CREAS), CONICYT-Regional, GORE Región de Valparaíso, Valparaíso, Chile; Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Caballero
authors.affiliation: Centro Regional de Estudios en Alimentos Saludables (CREAS), CONICYT-Regional, GORE Región de Valparaíso, Valparaíso, Chile; Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
authors.forename: Carmen
authors.initials: C
authors.lastname: Soto
authors.affiliation: Centro Regional de Estudios en Alimentos Saludables (CREAS), CONICYT-Regional, GORE Región de Valparaíso, Valparaíso, Chile; Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
authors.forename: Araceli
authors.initials: A
authors.lastname: Olivares
authors.affiliation: Centro Regional de Estudios en Alimentos Saludables (CREAS), CONICYT-Regional, GORE Región de Valparaíso, Valparaíso, Chile; Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
authors.forename: Claudia
authors.initials: C
authors.lastname: Altamirano
mesh_terms: D055162:Biocatalysis; D002210:Caprylates / Q000378:metabolism; D004041:Dietary Fats / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000378:metabolism; D004951:Esterification; D008049:Lipase / Q000378:metabolism*; D027421:Persea / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry* / Q000378:metabolism; D014280:Triglycerides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002210:Caprylates; D004041:Dietary Fats; D004800:Enzymes, Immobilized; D010938:Plant Oils; D014280:Triglycerides; C093666:Lipozyme; D008049:Lipase; C031492:octanoic acid