title: Oral cadmium exposure during rat pregnancy: assessment of transplacental micronutrient transport and steroidogenesis at term.
abstract: Diet is the main source of cadmium (Cd) exposure. Gastrointestinal absorption increases during pregnancy. Cadmium accumulated in the placenta may interfere with nutrient transport to the foetus. Data on the potential of Cd to act as a steroid disruptor of pregnancy are limited. We evaluated the effects of oral Cd exposure during pregnancy on placental function in micronutrient transfer to the foetus and steroidogenesis in Wistar rats (regular 4-day cyclers) that mated with unexposed males. Pregnant rats were randomly assigned to a Cd group exposed orally to 50 mg Cd l(-1) (CdCl(2)xH2O dissolved in demineralized water), ≈ 7.5 mg Cd kg(-1) a day, during 20 days of gestation and control (supplied with demineralized water). Non-pregnant rats were treated under the same experimental conditions. On day 20, all of the rats were killed and samples were taken for element analyses (by electrothermal atomic absorption spectrometry). Progesterone and testosterone were measured in serum and placenta-derived samples (by immunoenzymometric assay and/or enzyme-linked immunosorbent assay). In the exposed rats, Cd increased in blood and organs, more in pregnant rats, and in placenta and foetus whereas zinc increased in liver. Iron decreased in maternal organs and in foetus, whereas zinc decreased in maternal kidney and placenta. Liver copper was lower and kidney copper higher in all pregnant vs. non-pregnant rats. Steroids in serum and placenta did not change. In conclusion, oral Cd exposure during rat pregnancy does not affect progesterone and testosterone at term. Transplacental iron and zinc handover are disrupted, which may put at risk the maintenance of foetal nutrition and viability.
authors.affiliation: Institute for Medical Research and Occupational Health, Zagreb, Croatia.
authors.forename: Anja
authors.initials: A
authors.lastname: Mikolić
authors.forename: Martina
authors.initials: M
authors.lastname: Piasek
authors.forename: Antonija
authors.initials: A
authors.lastname: Sulimanec Grgec
authors.forename: Veda M
authors.initials: VM
authors.lastname: Varnai
authors.forename: Sandra
authors.initials: S
authors.lastname: Stasenko
authors.forename: Saša
authors.initials: S
authors.lastname: Kralik Oguić
mesh_terms: D000818:Animals; D002104:Cadmium / Q000008:administration & dosage* / Q000493:pharmacokinetics; D003300:Copper / Q000493:pharmacokinetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005333:Fetus / Q000187:drug effects; D007501:Iron / Q000493:pharmacokinetics; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008297:Male; D018977:Micronutrients / Q000493:pharmacokinetics*; D010920:Placenta / Q000187:drug effects*; D011247:Pregnancy; D011374:Progesterone / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D013739:Testosterone / Q000097:blood*; D015032:Zinc / Q000493:pharmacokinetics
chemical_list: D018977:Micronutrients; D002104:Cadmium; D013739:Testosterone; D011374:Progesterone; D003300:Copper; D007501:Iron; D015032:Zinc
keywords: cadmium; copper; foetus; iron; placenta; pregnancy; progesterone; rats; testosterone; zinc