title: Application of zwitterionic detergent to the solubilization of Klebsiella pneumoniae outer membrane proteins for two-dimensional gel electrophoresis.
abstract: Klebsiella pneumoniae is a frequent cause of nosocomial respiratory, urinary and gastrointestinal tract infections and septicemia with the multidrug-resistant K. pneumoniae being a major public health concern. Outer membrane proteins (OMPs) are important virulence factors responsible for the appropriate adaptation to the host environment. They constitute of the antigens being the first in contact with infected organism. However, K. pneumoniae strains are heavily capsulated and it is important to establish the OMPs isolation procedure prior to proteomics extensive studies. In this study we used Zwittergent Z 3-14® as a detergent to isolate the OMPs from K. pneumoniae cells and resolve them using two-dimensional electrophoresis (2-DE). As a result we identified 134 protein spots. The OMPs identified in this study are possible candidates for the development of a protein-based vaccine against K. pneumoniae infections.
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chałubińskiego 10, 50-368 Wrocalaw, Poland. Electronic address: iwona.bednarz-misa@umed.wroc.pl.
authors.forename: I
authors.initials: I
authors.lastname: Bednarz-Misa
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chałubińskiego 10, 50-368 Wrocalaw, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Serek
authors.affiliation: Department of Microbiology, Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Dudek
authors.affiliation: Department of Microbiology, Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Pawlak
authors.affiliation: Department of Microbiology, Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland.
authors.forename: G
authors.initials: G
authors.lastname: Bugla-Płoskońska
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chałubińskiego 10, 50-368 Wrocalaw, Poland; Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wroclaw, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Gamian
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D003902:Detergents / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D007711:Klebsiella pneumoniae / Q000737:chemistry*; D012995:Solubility
chemical_list: D001425:Bacterial Outer Membrane Proteins; D003902:Detergents
keywords: Klebsiella pneumoniae; Outer membrane proteins; Two-dimensional electrophoresis; Zwitterionic detergent