title: Characteristics of circulating CD31(+) cells from patients with coronary artery disease.
abstract: Recently, we reported the properties of CD31-expressing cells in healthy individuals. However, the characteristics of CD31-expressing cells derived from coronary artery disease (CAD) patients remain unknown. This study aimed to investigate the relationship between circulating CD31(+) cells and CAD as well as their biological characteristics. Analysis with flow cytometry revealed that CD31(+) cells (C-CD31) from the peripheral blood (PB) of CAD patients exhibited low levels of T-cell marker and high levels of macrophage marker compared with the PB-CD31(+) cells from healthy individuals (H-CD31). In addition, the expression levels of multiple pro-angiogenic and chemokine genes were significantly down-regulated in C-CD31. However, inflammatory gene IL-1α was highly up-regulated in C-CD31. Patients with unstable angina (UA) had significantly more CD31(+) cells in the PB than healthy control group (P < 0.001). Moreover, there were significant correlations between the number of CD31(+) cells and cardiovascular (CV) disease activity (R = 0.318, P = 0.006) and the number of diseased coronaries (R = 0.312, P = 0.005). For the diagnostic category of UA, the area under curve was 0.803 (P < 0.001). In conclusion, C-CD31 have impaired angiogenic potential and the number of circulating CD31(+) cells were correlated with CV risk. These findings may contribute to the understanding of the pathogenesis of CAD.
authors.affiliation: Department of Cardiology, College of Medicine, Dong-A University, Busan, Korea; Regional Clinical Trial Center, Dong-A University Hospital, Busan, Korea.
authors.forename: Moo Hyun
authors.initials: MH
authors.lastname: Kim
authors.forename: Longzhe
authors.initials: L
authors.lastname: Guo
authors.forename: Han-Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Sung-Whan
authors.initials: SW
authors.lastname: Kim
mesh_terms: D000368:Aged; D000787:Angina Pectoris / Q000097:blood* / Q000473:pathology; D003324:Coronary Artery Disease / Q000097:blood* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007249:Inflammation / Q000097:blood* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis* / Q000097:blood
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1
keywords: CD31 antigen; angina pectoris; angiogenesis effect; coronary artery disease; inflammation