title: Invasive reaction assisted strand-displacement signal amplification for sensitive DNA detection.
abstract: A novel DNA detection assay was proposed by invasive reaction coupled with molecular beacon assisted strand-displacement signal amplification (IRASA). Target DNAs are firstly hybridized to two probes to initiate invasive reaction to produce amplified flaps. Then these flaps are further amplified by strand-displacement signal amplification. The detection limit was around 0.2 pM.
authors.affiliation: Department of Pharmacology, Jinling Hospital, Medical School of Nanjing University, Nanjing, 210002, China. ghzhou@nju.edu.cn.
authors.forename: Bingjie
authors.initials: B
authors.lastname: Zou
authors.forename: Qinxin
authors.initials: Q
authors.lastname: Song
authors.forename: Jianping
authors.initials: J
authors.lastname: Wang
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Liu
authors.forename: Guohua
authors.initials: G
authors.lastname: Zhou
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics; D004247:DNA / Q000032:analysis*; D066246:ErbB Receptors / Q000235:genetics; D006801:Humans; D057230:Limit of Detection; D008175:Lung Neoplasms / Q000235:genetics; D009154:Mutation; D021141:Nucleic Acid Amplification Techniques; D009693:Nucleic Acid Hybridization
chemical_list: D004247:DNA; C512478:EGFR protein, human; D066246:ErbB Receptors