title: Signal transducer and oxidative stress mediated modulation of phenylpropanoid pathway to enhance rosmarinic acid biosynthesis in fungi elicited whole plant culture of Solenostemon scutellarioides.
abstract: This study aimed to improve rosmarinic acid (RA) production in the whole plant culture of Solenostemon scutellarioides through elicitation with phytopathogenic fungi. Amongst selected fungi, Aternaria alternata caused significant elevation (p<0.05-0.01) in RA accumulation (∼1.3-1.6-fold) between 25 and 100 μg l(-1). However, elicitation at the dose of 50 μg l(-1) has been found to be most effective and intracellular RA content reached almost ∼1.6-fold (p<0.01) higher in day 7. Therefore, A. alternata (50 μg l(-1)) was selected for mechanism evaluation. A significant elevation of intercellular jasmonic acid was observed up to day 6 after elicitation with A. alternata (50 μg l(-1)). A significant increase in tissue H2O2 and lipid peroxidation coupled with depletion of antioxidant enzymes superoxide dismutase and catalase indicated augmented oxidative stress associated with biotic interaction. Preceding the elicitor-induced RA accumulation, a notable alteration in the specific activities of biosynthetic enzymes namely PAL and TAT was recorded, while, no significant change in the activities of RAS was observed. HPPR activity was slightly improved in elicited plant. Therefore, it could be concluded that A. alternata elicited the biosynthesis of rosmarinic acid via signal transduction through jasmonic acid coupled with elicitor induced oxidative stress and associated mechanism.
authors.affiliation: Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, West Bengal, India. Electronic address: s.dewanjee@yahoo.com.
authors.forename: Saikat
authors.initials: S
authors.lastname: Dewanjee
authors.affiliation: Biophysics and Structural Genomics Division, Saha Institute of Nuclear Physics, Kolkata 700064, West Bengal, India.
authors.forename: Moumita
authors.initials: M
authors.lastname: Gangopadhyay
authors.affiliation: Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, West Bengal, India.
authors.forename: Urmi
authors.initials: U
authors.lastname: Das
authors.affiliation: Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, West Bengal, India.
authors.forename: Ranabir
authors.initials: R
authors.lastname: Sahu
authors.affiliation: Division of Microbiology, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, West Bengal, India.
authors.forename: Amalesh
authors.initials: A
authors.lastname: Samanta
authors.affiliation: Department of Environmental Science, University of Calcutta, Kolkata 700019, India.
authors.forename: Pamela
authors.initials: P
authors.lastname: Banerjee
mesh_terms: D000528:Alternaria / Q000472:pathogenicity; D000975:Antioxidants / Q000378:metabolism; D001234:Aspergillus niger / Q000472:pathogenicity; D002934:Cinnamates / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism; D053630:Depsides / Q000378:metabolism*; D005658:Fungi / Q000472:pathogenicity*; D005670:Fusarium / Q000472:pathogenicity; D017343:Genes, Plant; D019686:Lamiaceae / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D053858:Metabolic Networks and Pathways; D018384:Oxidative Stress; D054883:Oxylipins / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014444:Tyrosine Transaminase / Q000235:genetics / Q000378:metabolism; D000097123:Rosmarinic Acid
chemical_list: D000975:Antioxidants; D002934:Cinnamates; D003517:Cyclopentanes; D053630:Depsides; D054883:Oxylipins; D010650:Phenylalanine Ammonia-Lyase; D010940:Plant Proteins; D014444:Tyrosine Transaminase; D000097123:Rosmarinic Acid; C011006:jasmonic acid
keywords: Aternaria alternata; Fungi elicitors; Rosmarinic acid; Semi-quantitative RT-PCR; Solenostemon scutellarioides