title: Association of polymorphisms in the endocannabinoid system genes with myocardial infarction and plasma cholesterol levels.
abstract: OBJECTIVE
  The aim of this study was to investigate the relationship between selected symptoms of chronic heart failure (myocardial infarction, plasma cholesterol level) and single nucleotide polymorphisms (SNPs) in the FAAH and CNR1 genes.
  
  
  METHODS
  A case - control study involving 155 patients with chronic heart failure and 169 age- and sex-matched healthy subjects. We detected SNPs 385 C/A (rs324420) in the FAAH and 1359 G/A (rs1049353) in the CNR1 genes using the polymerase chain reaction and restriction analysis. Genotype and allele frequencies were compared between patients and controls as well as between patients with and without myocardial infarction.
  
  
  RESULTS
  No significant differences in genotype or allelic frequencies between patients and controls were found (P > 0.05). Carriers of the FAAH A allele had a 2.37-fold increase in the risk of myocardial infarction (odds ratio 2.37, 95% confidence interval 1.36-6.93, P = 0.01). Homozygous carriers of genotype AA of CNR1 SNP 1359 had significantly higher plasma cholesterol levels than carriers of GG and GA genotypes in patients (P = 0.04).
  
  
  CONCLUSIONS
  The study results suggest a role for allele A of the FAAH 385 variant as a risk factor for myocardial infarction. Genotype AA of CNR1 1359 variant probably affects plasma cholesterol levels. Pharmacological intervention in this system could modify the therapeutic approach to certain cardiovascular disorders.
authors.affiliation: Department of Pathophysiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: Monika
authors.initials: M
authors.lastname: Chmelikova
authors.affiliation: Department of Pathophysiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: Lukas
authors.initials: L
authors.lastname: Pacal
authors.affiliation: Department of Cardioangiology, St. Anne´s University Hospital in Brno.
authors.forename: Lenka
authors.initials: L
authors.lastname: Spinarova
authors.affiliation: Department of Pathophysiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: Anna
authors.initials: A
authors.lastname: Vasku
mesh_terms: D000581:Amidohydrolases / Q000235:genetics*; D000704:Analysis of Variance; D002784:Cholesterol / Q000378:metabolism*; D002908:Chronic Disease; D063388:Endocannabinoids / Q000235:genetics*; D005260:Female; D005838:Genotype; D006333:Heart Failure / Q000235:genetics; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D043884:Receptor, Cannabinoid, CB1 / Q000235:genetics*; D000099252:Fatty Acid Amide Hydrolases
chemical_list: D000581:Amidohydrolases; D002784:Cholesterol; D063388:Endocannabinoids; D043884:Receptor, Cannabinoid, CB1; D000099252:Fatty Acid Amide Hydrolases; C571130:CNR1 protein, human
keywords: cannabinoid receptor; cholesterol; chronic heart failure; endocannabinoid system; fatty acid amide hydrolase; myocardial infarction