title: Altered mitochondrial function and oxidative stress in leukocytes of anorexia nervosa patients.
abstract: BACKGROUND
  Anorexia nervosa is a common illness among adolescents and is characterised by oxidative stress.
  
  
  OBJECTIVE
  The effects of anorexia on mitochondrial function and redox state in leukocytes from anorexic subjects were evaluated.
  
  
  METHODS
  A multi-centre, cross-sectional case-control study was performed.
  
  
  METHODS
  Our study population consisted of 20 anorexic patients and 20 age-matched controls, all of which were Caucasian women.
  
  
  METHODS
  Anthropometric and metabolic parameters were evaluated in the study population. To assess whether anorexia nervosa affects mitochondrial function and redox state in leukocytes of anorexic patients, we measured mitochondrial oxygen consumption, membrane potential, reactive oxygen species production, glutathione levels, mitochondrial mass, and complex I and III activity in polymorphonuclear cells.
  
  
  RESULTS
  Mitochondrial function was impaired in the leukocytes of the anorexic patients. This was evident in a decrease in mitochondrial O2 consumption (P<0.05), mitochondrial membrane potential (P<0.01) and GSH levels (P<0.05), and an increase in ROS production (P<0.05) with respect to control subjects. Furthermore, a reduction of mitochondrial mass was detected in leukocytes of the anorexic patients (P<0.05), while the activity of mitochondrial complex I (P<0.001), but not that of complex III, was found to be inhibited in the same population.
  
  
  CONCLUSIONS
  Oxidative stress is produced in the leukocytes of anorexic patients and is closely related to mitochondrial dysfunction. Our results lead us to propose that the oxidative stress that occurs in anorexia takes place at mitochondrial complex I. Future research concerning mitochondrial dysfunction and oxidative stress should aim to determine the physiological mechanism involved in this effect and the physiological impact of anorexia.
authors.affiliation: Foundation for the Promotion of Healthcare and Biomedical Research in the Valencian Community (FISABIO), Valencia, Spain; Service of Endocrinology, University Hospital Doctor Peset, Valencia, Spain; Institute of Health Research INCLIVA, University of Valencia, Valencia, Spain.
authors.forename: Victor M
authors.initials: VM
authors.lastname: Victor
authors.affiliation: Foundation for the Promotion of Healthcare and Biomedical Research in the Valencian Community (FISABIO), Valencia, Spain; Service of Endocrinology, University Hospital Doctor Peset, Valencia, Spain; Institute of Health Research INCLIVA, University of Valencia, Valencia, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Rovira-Llopis
authors.affiliation: Psychiatry Service, University Hospital La Fe, Department of Medicine, University of Valencia, Valencia, Spain.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Saiz-Alarcon
authors.affiliation: Psychiatry Service, University Hospital La Fe, Department of Medicine, University of Valencia, Valencia, Spain.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Sangüesa
authors.affiliation: Psychiatry Service, University Hospital La Fe, Department of Medicine, University of Valencia, Valencia, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Rojo-Bofill
authors.affiliation: Foundation for the Promotion of Healthcare and Biomedical Research in the Valencian Community (FISABIO), Valencia, Spain; Service of Endocrinology, University Hospital Doctor Peset, Valencia, Spain; Institute of Health Research INCLIVA, University of Valencia, Valencia, Spain.
authors.forename: Celia
authors.initials: C
authors.lastname: Bañuls
authors.affiliation: Foundation for the Promotion of Healthcare and Biomedical Research in the Valencian Community (FISABIO), Valencia, Spain; Service of Endocrinology, University Hospital Doctor Peset, Valencia, Spain; Institute of Health Research INCLIVA, University of Valencia, Valencia, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Falcón
authors.affiliation: Foundation for the Promotion of Healthcare and Biomedical Research in the Valencian Community (FISABIO), Valencia, Spain; Service of Endocrinology, University Hospital Doctor Peset, Valencia, Spain; Institute of Health Research INCLIVA, University of Valencia, Valencia, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Castelló
authors.affiliation: Psychiatry Service, University Hospital La Fe, Department of Medicine, University of Valencia, Valencia, Spain; Research Group CIBER CB/06/02/0045, CIBER actions, Epidemiology and Public Health, University of Valencia, Valencia, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Rojo
authors.affiliation: Foundation for the Promotion of Healthcare and Biomedical Research in the Valencian Community (FISABIO), Valencia, Spain; Service of Endocrinology, University Hospital Doctor Peset, Valencia, Spain; Institute of Health Research INCLIVA, University of Valencia, Valencia, Spain.
authors.forename: Milagros
authors.initials: M
authors.lastname: Rocha
authors.affiliation: Foundation for the Promotion of Healthcare and Biomedical Research in the Valencian Community (FISABIO), Valencia, Spain; Service of Endocrinology, University Hospital Doctor Peset, Valencia, Spain; Institute of Health Research INCLIVA, University of Valencia, Valencia, Spain; Department of Medicine, University of Valencia, Valencia, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Hernández-Mijares
mesh_terms: D000293:Adolescent; D000328:Adult; D000856:Anorexia Nervosa / Q000378:metabolism* / Q000473:pathology*; D042967:Electron Transport Complex I / Q000378:metabolism; D014450:Electron Transport Complex III / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology; D053142:Mitochondrial Size; D018384:Oxidative Stress; D010101:Oxygen Consumption; D017382:Reactive Oxygen Species / Q000378:metabolism; D055815:Young Adult
chemical_list: D017382:Reactive Oxygen Species; D042967:Electron Transport Complex I; D014450:Electron Transport Complex III; D005978:Glutathione