title: Expression of ORAII, a plasma membrane resident subunit of the CRAC channel, in rodent and non-rodent species.
abstract: We determined the expression of ORAI1 protein in rodent and non-rodent tissues using a monoclonal antibody directed against an extracellular loop of the protein. Previous reports using antibodies directed at the C-terminus of ORAI1 have not detected central nervous system (CNS) expression. Our results demonstrate broad tissue expression that includes the CNS using a unique monoclonal antibody specific to an extracellular loop of ORAI1. In addition, we present in situ hybridization (ISH) results using a probe within the middle of the mouse coding region showing CNS expression of Orai1 RNA. We contrast the patterns of rodent and human tissue expression and conclude that rodents have similar expression of ORAI1 in most tissue types when compared to primates, with an important exception being the male reproductive system, where human-specific expression is observed.
authors.affiliation: Departments of Pathology (RG, EGV, JP, EP, MQ), Amgen, Inc. Thousand Oaks, CaliforniaTherapeutic Discovery (FFL, VB), Amgen, Inc. Thousand Oaks, CaliforniaDiscovery Toxicology (HJM), Amgen, Inc. Thousand Oaks, CaliforniaInflammation Research (BDB, DHF), Amgen, Inc. Thousand Oaks, California.
authors.forename: Roberto
authors.initials: R
authors.lastname: Guzman
authors.affiliation: Departments of Pathology (RG, EGV, JP, EP, MQ), Amgen, Inc. Thousand Oaks, CaliforniaTherapeutic Discovery (FFL, VB), Amgen, Inc. Thousand Oaks, CaliforniaDiscovery Toxicology (HJM), Amgen, Inc. Thousand Oaks, CaliforniaInflammation Research (BDB, DHF), Amgen, Inc. Thousand Oaks, California.
authors.forename: Eliane G
authors.initials: EG
authors.lastname: Valente
authors.affiliation: Departments of Pathology (RG, EGV, JP, EP, MQ), Amgen, Inc. Thousand Oaks, CaliforniaTherapeutic Discovery (FFL, VB), Amgen, Inc. Thousand Oaks, CaliforniaDiscovery Toxicology (HJM), Amgen, Inc. Thousand Oaks, CaliforniaInflammation Research (BDB, DHF), Amgen, Inc. Thousand Oaks, California.
authors.forename: Jim
authors.initials: J
authors.lastname: Pretorius
authors.affiliation: Departments of Pathology (RG, EGV, JP, EP, MQ), Amgen, Inc. Thousand Oaks, CaliforniaTherapeutic Discovery (FFL, VB), Amgen, Inc. Thousand Oaks, CaliforniaDiscovery Toxicology (HJM), Amgen, Inc. Thousand Oaks, CaliforniaInflammation Research (BDB, DHF), Amgen, Inc. Thousand Oaks, California.
authors.forename: Efrain
authors.initials: E
authors.lastname: Pacheco
authors.affiliation: Departments of Pathology (RG, EGV, JP, EP, MQ), Amgen, Inc. Thousand Oaks, CaliforniaTherapeutic Discovery (FFL, VB), Amgen, Inc. Thousand Oaks, CaliforniaDiscovery Toxicology (HJM), Amgen, Inc. Thousand Oaks, CaliforniaInflammation Research (BDB, DHF), Amgen, Inc. Thousand Oaks, California.
authors.forename: Meiying
authors.initials: M
authors.lastname: Qi
authors.affiliation: Departments of Pathology (RG, EGV, JP, EP, MQ), Amgen, Inc. Thousand Oaks, CaliforniaTherapeutic Discovery (FFL, VB), Amgen, Inc. Thousand Oaks, CaliforniaDiscovery Toxicology (HJM), Amgen, Inc. Thousand Oaks, CaliforniaInflammation Research (BDB, DHF), Amgen, Inc. Thousand Oaks, California.
authors.forename: Brian D
authors.initials: BD
authors.lastname: Bennett
authors.affiliation: Departments of Pathology (RG, EGV, JP, EP, MQ), Amgen, Inc. Thousand Oaks, CaliforniaTherapeutic Discovery (FFL, VB), Amgen, Inc. Thousand Oaks, CaliforniaDiscovery Toxicology (HJM), Amgen, Inc. Thousand Oaks, CaliforniaInflammation Research (BDB, DHF), Amgen, Inc. Thousand Oaks, California.
authors.forename: David H
authors.initials: DH
authors.lastname: Fong
authors.affiliation: Departments of Pathology (RG, EGV, JP, EP, MQ), Amgen, Inc. Thousand Oaks, CaliforniaTherapeutic Discovery (FFL, VB), Amgen, Inc. Thousand Oaks, CaliforniaDiscovery Toxicology (HJM), Amgen, Inc. Thousand Oaks, CaliforniaInflammation Research (BDB, DHF), Amgen, Inc. Thousand Oaks, California.
authors.forename: Fen-Fen
authors.initials: FF
authors.lastname: Lin
authors.affiliation: Departments of Pathology (RG, EGV, JP, EP, MQ), Amgen, Inc. Thousand Oaks, CaliforniaTherapeutic Discovery (FFL, VB), Amgen, Inc. Thousand Oaks, CaliforniaDiscovery Toxicology (HJM), Amgen, Inc. Thousand Oaks, CaliforniaInflammation Research (BDB, DHF), Amgen, Inc. Thousand Oaks, California.
authors.forename: Vivian
authors.initials: V
authors.lastname: Bi
authors.affiliation: Departments of Pathology (RG, EGV, JP, EP, MQ), Amgen, Inc. Thousand Oaks, CaliforniaTherapeutic Discovery (FFL, VB), Amgen, Inc. Thousand Oaks, CaliforniaDiscovery Toxicology (HJM), Amgen, Inc. Thousand Oaks, CaliforniaInflammation Research (BDB, DHF), Amgen, Inc. Thousand Oaks, California hmcbride@amgen.com.
authors.forename: Helen J
authors.initials: HJ
authors.lastname: McBride
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D015220:Calcium Channels / Q000032:analysis* / Q000235:genetics; D002460:Cell Line; D002490:Central Nervous System / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D017403:In Situ Hybridization / Q000379:methods; D008252:Macaca fascicularis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D000071740:ORAI1 Protein; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D046888:Tissue Array Analysis / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D015220:Calcium Channels; D000071740:ORAI1 Protein; C510746:ORAI1 protein, human; C520430:Orai1 protein, mouse; C532493:Orai1 protein, rat; D012333:RNA, Messenger
keywords: CRAC; CRACM1; IHC; ISH; ORAI1; TMEM142a; mAb266.1