title: A new flavanone and other flavonoids from green perilla leaf extract inhibit nitric oxide production in interleukin 1β-treated hepatocytes.
abstract: A new flavanone, shisoflavanone A (1), and several flavonoids were purified from the ethyl acetate-soluble fraction of green perilla leaves (Perilla frutescens Britton var. crispa form viridis), and their structures were identified. Shisoflavanone A was elucidated as 8-hydroxy-6,7-dimethoxyflavanone based on its spectral data. Other constituents of the ethyl acetate-soluble fraction, i.e. 5,8-dihydroxy-7-methoxyflavanone (2), negletein (5,6-dihydroxy-7-methoxyflavone) (3), luteolin (4), apigenin (5), esculetin (6), and protocatechuic acid (7), were identified. This is the first time that constituents 2, 3, and 6 have been found in green perilla. Shisoflavanone A and the other constituents (except 7) significantly inhibited nitric oxide production in interleukin 1β-stimulated rat hepatocytes, which have been used to monitor the anti-inflammatory effects of herbal constituents. The present findings suggest that these constituents, including shisoflavanone A, may be involved in the anti-inflammatory effects of green perilla leaves.
authors.affiliation: a Department of Pharmacy , College of Pharmaceutical Sciences, Ritsumeikan University , Kusatsu , Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Nakajima
authors.forename: Yuka
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Nao
authors.initials: N
authors.lastname: Yoshinaka
authors.forename: Mayuri
authors.initials: M
authors.lastname: Namba
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Matsuo
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Okuyama
authors.forename: Emi
authors.initials: E
authors.lastname: Yoshigai
authors.forename: Tadayoshi
authors.initials: T
authors.lastname: Okumura
authors.forename: Mikio
authors.initials: M
authors.lastname: Nishizawa
authors.forename: Yukinobu
authors.initials: Y
authors.lastname: Ikeya
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D047310:Apigenin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D044950:Flavanones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D047309:Flavones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors / Q000494:pharmacology; D047311:Luteolin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D018384:Oxidative Stress; D031344:Perilla frutescens / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D061251:Primary Cell Culture; D051381:Rats; D017208:Rats, Wistar; D014468:Umbelliferones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D044950:Flavanones; D047309:Flavones; D005419:Flavonoids; D053583:Interleukin-1beta; D010936:Plant Extracts; D014468:Umbelliferones; C507557:negletein; C000601822:shisoflavanone A; D009569:Nitric Oxide; D047310:Apigenin; D047311:Luteolin; C007628:esculetin
keywords: chemokine; flavanone; flavone; green perilla; nitric oxide