title: Quantitative analysis of pork and chicken products by droplet digital PCR.
abstract: In this project, a highly precise quantitative method based on the digital polymerase chain reaction (dPCR) technique was developed to determine the weight of pork and chicken in meat products. Real-time quantitative polymerase chain reaction (qPCR) is currently used for quantitative molecular analysis of the presence of species-specific DNAs in meat products. However, it is limited in amplification efficiency and relies on standard curves based Ct values, detecting and quantifying low copy number target DNA, as in some complex mixture meat products. By using the dPCR method, we find the relationships between the raw meat weight and DNA weight and between the DNA weight and DNA copy number were both close to linear. This enabled us to establish formulae to calculate the raw meat weight based on the DNA copy number. The accuracy and applicability of this method were tested and verified using samples of pork and chicken powder mixed in known proportions. Quantitative analysis indicated that dPCR is highly precise in quantifying pork and chicken in meat products and therefore has the potential to be used in routine analysis by government regulators and quality control departments of commercial food and feed enterprises.
authors.affiliation: Technical Center for Animal, Plant and Food Inspection and Quarantine, Shanghai Entry-Exit Inspection and Quarantine Bureau of China, No. 1208, Minsheng Road, Pudong New Area, Shanghai 200135, China.
authors.forename: Yicun
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Technical Center for Animal, Plant and Food Inspection and Quarantine, Shanghai Entry-Exit Inspection and Quarantine Bureau of China, No. 1208, Minsheng Road, Pudong New Area, Shanghai 200135, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: Technical Center for Animal, Plant and Food Inspection and Quarantine, Shanghai Entry-Exit Inspection and Quarantine Bureau of China, No. 1208, Minsheng Road, Pudong New Area, Shanghai 200135, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Lv
authors.affiliation: Technical Center for Animal, Plant and Food Inspection and Quarantine, Shanghai Entry-Exit Inspection and Quarantine Bureau of China, No. 1208, Minsheng Road, Pudong New Area, Shanghai 200135, China.
authors.forename: Jielin
authors.initials: J
authors.lastname: Yang
authors.affiliation: Technical Center for Animal, Plant and Food Inspection and Quarantine, Shanghai Entry-Exit Inspection and Quarantine Bureau of China, No. 1208, Minsheng Road, Pudong New Area, Shanghai 200135, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Technical Center for Animal, Plant and Food Inspection and Quarantine, Shanghai Entry-Exit Inspection and Quarantine Bureau of China, No. 1208, Minsheng Road, Pudong New Area, Shanghai 200135, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: He
authors.affiliation: Technical Center for Animal, Plant and Food Inspection and Quarantine, Shanghai Entry-Exit Inspection and Quarantine Bureau of China, No. 1208, Minsheng Road, Pudong New Area, Shanghai 200135, China.
authors.forename: Liangwen
authors.initials: L
authors.lastname: Pan
mesh_terms: D000818:Animals; D002645:Chickens; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D016014:Linear Models; D008460:Meat / Q000032:analysis* / Q000145:classification*; D008461:Meat Products; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013552:Swine
chemical_list: D004247:DNA