title: Dental pulp cells that express adeno-associated virus serotype 2-mediated BMP-7 gene enhanced odontoblastic differentiation.
abstract: This present study investigated the potential of adeno-associated virus serotype 2 (AAV2) mediated BMP-7 (AAV2-BMP-7) to induce odontoblastic differentiation of human dental pulp cells (DPCs) in vitro. AAV2-BMP-7 was constructed to overexpress BMP-7, and the biologic effects of BMP-7 on DPCs were investigated by the evaluation of the activity of alkaline phosphatase (ALPase), the detection of the expression of dentin sialophosphoprotein (DSPP) and osteocalcin (OCN) expression and the analysis of the proliferative ability of the cells. DPCs that were infected with AAV2-BMP-7 displayed significantly upregulated ALP activity and formed mineralized nodules. Moreover, AAV2-BMP-7 promoted the expression of mineralization-related genes, which included DSPP and OCN. In addition, there was no significant difference between the proliferative ability of AAV2-BMP-7 and the control group. In conclusion, AAV2-BMP-7 promoted the odontoblastic differentiation in DPCs, a clear indication of the therapeutic potential of AAV2-BMP-7 in dental tissue regeneration.
authors.affiliation: Department of Operative Dentistry and Endodontics, Guanghua School and Hospital of Stomatology & Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University.
authors.forename: Wei
authors.initials: W
authors.lastname: Qin
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhu
authors.forename: Lingling
authors.initials: L
authors.lastname: Chen
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Yang
authors.forename: Qiting
authors.initials: Q
authors.lastname: Huang
authors.forename: Zhengmei
authors.initials: Z
authors.lastname: Lin
mesh_terms: D055419:Bone Morphogenetic Protein 7 / Q000235:genetics*; D002454:Cell Differentiation; D003782:Dental Pulp / Q000166:cytology*; D000229:Dependovirus / Q000235:genetics*; D006801:Humans; D009804:Odontoblasts / Q000166:cytology*
chemical_list: D055419:Bone Morphogenetic Protein 7