title: Glycoproteomics approach for identifying Glycobiomarker candidate molecules for tissue type classification of non-small cell lung carcinoma.
abstract: Histopathological classification of lung cancer has important implications in the application of clinical practice guidelines and the prediction of patient prognosis. Thus, we focused on discovering glycobiomarker candidates to classify the types of lung cancer tissue. First, we performed lectin microarray analysis of lung cancer tissue specimens and cell lines and identified Aleuria aurantia lectin (AAL), Hippeastrum hybrid lectin (HHL), and Concanavalia ensiformis agglutinin (ConA) as lectin probes specific to non-small cell lung carcinoma (NSCLC). LC-MS-based analysis was performed for the comprehensive identification of glycoproteins and N-linked glycosylation sites using lectin affinity capture of NSCLC-specific glycoforms of glycoproteins. This analysis identified 1092 AAL-bound glycoproteins (316 gene symbols) and 948 HHL/ConA-bound glycoproteins (279 gene symbols). The lectin microarray-assisted verification using 15 lung cancer cell lines revealed the NSCLC-specific expression of fibronectin. The glycosylation profiling of fibronectin indicated that the peanut agglutinin (PNA) signal appeared to differentiate two NSCLC types, adenocarcinoma and large cell carcinoma, whereas the protein expression level was similar between these types. Our glycoproteomics approach together with the concurrent use of an antibody and lectin is applicable to the quantitative and qualitative monitoring of variations in glycosylation of fibronectin specific to certain types of lung cancer tissue.
authors.affiliation: Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology (AIST) , Central 2, 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan.
authors.forename: Yoshitoshi
authors.initials: Y
authors.lastname: Hirao
authors.forename: Hideki
authors.initials: H
authors.lastname: Matsuzaki
authors.forename: Jun
authors.initials: J
authors.lastname: Iwaki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kuno
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kaji
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohkura
authors.forename: Akira
authors.initials: A
authors.lastname: Togayachi
authors.forename: Minako
authors.initials: M
authors.lastname: Abe
authors.forename: Masaharu
authors.initials: M
authors.lastname: Nomura
authors.forename: Masayuki
authors.initials: M
authors.lastname: Noguchi
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Ikehara
authors.forename: Hisashi
authors.initials: H
authors.lastname: Narimatsu
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000145:classification* / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D005353:Fibronectins / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D037102:Lectins / Q000378:metabolism; D013058:Mass Spectrometry; D046228:Microarray Analysis; D019887:Peanut Agglutinin; D040901:Proteomics / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D005353:Fibronectins; D006023:Glycoproteins; D037102:Lectins; D019887:Peanut Agglutinin
keywords: Biomarker; glycoproteomics; glycosylation; lectin microarray; lung cancer; tissue