title: Development of a loop-mediated isothermal amplification assay for rapid detection of Nocardia salmonicida, the causative agent of nocardiosis in fish.
abstract: Nocardia salmonicida is one of the main pathogens of fish nocardiosis. The purpose of this study was to build a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of N. salmonicida. A set of four primers were designed from the 16S-23S rRNA intergenic spacer region of N. salmonicida, and conditions for LAMP were optimized as incubating all the reagents for 60 min at 64°C. LAMP products were judged with agar gel electrophoresis as well as with the naked eye after the addition of SYBR Green I. Results showed the sensitivity of the LAMP assay was 1.68 × 10(3) CFU/ml (16.8 CFU per reaction) and 10-fold higher than that of PCR. The LAMP method was also effectively applied to detect N. salmonicida in diseased fish samples, and it may potentially facilitate the surveillance and early diagnosis of fish nocardiosis.
authors.affiliation: Fisheries College, Guangdong Ocean University, Zhanjiang 524025, P.R. China, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, P.R. China, Key Laboratory of Diseases Controlling for Aquatic Economic Animals of Guangdong Higher Education Institutions, Zhanjiang 524088, P.R. China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Xia
authors.forename: Honglian
authors.initials: H
authors.lastname: Zhang
authors.forename: Yishan
authors.initials: Y
authors.lastname: Lu
authors.forename: Jia
authors.initials: J
authors.lastname: Cai
authors.forename: Bei
authors.initials: B
authors.lastname: Wang
authors.forename: Jichang
authors.initials: J
authors.lastname: Jian
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005393:Fish Diseases / Q000175:diagnosis*; D009615:Nocardia / Q000235:genetics* / Q000302:isolation & purification*; D009617:Nocardia Infections / Q000175:diagnosis* / Q000382:microbiology; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S