title: Thiol-based redox proteomics in cancer research.
abstract: Cancer cells maintain their intracellular ROS concentrations at required levels for their survival. Changes in ROS concentrations can regulate biochemical signaling mechanisms that control cell function. It has been demonstrated that ROS regulate the cellular events through redox regulation of redox-sensitive proteins (redox sensors). Upon oxidative stress, redox sensors undergo redox modifications that cause the allosteric changes of these proteins and endow them with different functions. Understanding the altered functions of redox sensors and the underlying mechanisms is critical for the development of novel cancer therapeutics. Recently, a series of high-throughput proteomics approaches have been developed for screening redox processes. In this manuscript, we review these methodologies and discuss the important redox sensors recently identified that are related to cancer.
authors.affiliation: The State Key Laboratory for Biotherapy, West China Hospital, Sichuan University, Chengdu, P. R. China.
authors.forename: Kefei
authors.initials: K
authors.lastname: Yuan
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Hai-Ning
authors.initials: HN
authors.lastname: Chen
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Jiang
authors.initials: J
authors.lastname: Lan
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.forename: Qianhui
authors.initials: Q
authors.lastname: Dou
authors.forename: Edouard C
authors.initials: EC
authors.lastname: Nice
authors.forename: Canhua
authors.initials: C
authors.lastname: Huang
mesh_terms: D000818:Animals; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009369:Neoplasms / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism*
chemical_list: D011506:Proteins; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds
keywords: Biomedicine; Cancer biomarkers; Comparative proteomics; Mass spectrometry; Qxidative stress; Signal transduction