title: Complete genomic sequence of a new Human polyomavirus 9 strain with an altered noncoding control region.
abstract: A complete Human polyomavirus 9 (HPyV9) genome, designated HPyV9 UF-1, was amplified by rolling circle DNA amplification from DNA extracted from the peripheral blood mononuclear cells (PBMC) of an AIDS patient. The noncoding control (enhancer/promoter) region (NCCR) of HPyV9 UF-1 has one less AML-1a binding site and three more potential Sp1/GC box binding sites than the NCCRs of two previously described HPyV9 genomes. Nucleotide polymorphisms within the coding regions result in two amino acid differences in the deduced VP2 and VP3 proteins of HPyV9 UF-1 relative to those of the two previously described HPyV9 genomes. Exhaustive attempts to detect HPyV9 in DNA samples extracted from the PBMC of 40 healthy humans and 9 other AIDS patients were unsuccessful, highlighting the need for improved search strategies and optimal specimens for the detection of HPyV9 in humans.
authors.affiliation: Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Box 100188, Gainesville, FL, 32610-0188, USA, jlednicky@phhp.ufl.edu.
authors.forename: John A
authors.initials: JA
authors.lastname: Lednicky
authors.forename: Janet S
authors.initials: JS
authors.lastname: Butel
authors.forename: Maya C
authors.initials: MC
authors.lastname: Luetke
authors.forename: Julia C
authors.initials: JC
authors.lastname: Loeb
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D001483:Base Sequence; D001665:Binding Sites; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D016679:Genome, Viral; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D019901:Locus Control Region; D008969:Molecular Sequence Data; D011120:Polyomavirus / Q000235:genetics* / Q000302:isolation & purification; D027601:Polyomavirus Infections / Q000821:virology; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral