title: Human papillomavirus among women with atypical squamous cells of undetermined significance in southern Brazil.
abstract: OBJECTIVE
  To determine the prevalence of atypical squamous cells of undetermined significance (ASCUS) and human papillomavirus (HPV) genotypes in a population in southern Brazil.
  
  
  METHODS
  In a retrospective cross-sectional study, the prevalence of ASCUS was determined among women aged 20-60 years who were referred to a private medical center in Caxias do Sul by a gynecologist for assessment of a cervical condition between January 1, 2010, and September 30, 2011. Histologic and cytologic samples were tested for HPV, and polymerase chain reaction (PCR) was used to genotype any HPV DNA identified.
  
  
  RESULTS
  Among the 250 included women, 25 (10.0%) had ASCUS. HPV DNA was found in 15 (60.0%) women with ASCUS and 115 (51.1%) of the 225 without ASCUS. Viral typing showed that 7 (46.7%) HPV-positive women with ASCUS had multiple infections with up to five different genotypes. Both low- and high-risk HPV genotypes were found in ASCUS samples; the most prevalent genotypes were HPV6/HPV11 (affecting 10 [66.7%] women), HPV51 (6 [40.0%]), and HPV16 (6 [40.0%]).
  
  
  CONCLUSIONS
  ASCUS is not an indication of HPV infection. HPV screening and genotyping would benefit women with ASCUS, because treatment can be planned according to risk of carcinogenesis.
authors.affiliation: Molecular Diagnostics Laboratory, Biotechnology Institute, Universidade de Caxias do Sul, Caxias do Sul, Brazil. Electronic address: sopaesi@ucs.br.
authors.forename: Suelen
authors.initials: S
authors.lastname: Paesi
authors.affiliation: Molecular Diagnostics Laboratory, Biotechnology Institute, Universidade de Caxias do Sul, Caxias do Sul, Brazil.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Correa
authors.affiliation: Center of Medical Pathology of Caxias do Sul, Caxias do Sul, Brazil.
authors.forename: Márcia Caldart
authors.initials: MC
authors.lastname: Tregnago
authors.affiliation: Molecular Diagnostics Laboratory, Biotechnology Institute, Universidade de Caxias do Sul, Caxias do Sul, Brazil.
authors.forename: Jovana
authors.initials: J
authors.lastname: Mandelli
authors.affiliation: Laboratory of Genomics, Proteomics and DNA Repair, Biotechnology Institute, Universidade de Caxias do Sul, Caxias do Sul, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: Roesch-Ely
mesh_terms: D000328:Adult; D065309:Atypical Squamous Cells of the Cervix; D001938:Brazil / Q000453:epidemiology; D060085:Coinfection / Q000821:virology; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005838:Genotype; D052140:Human papillomavirus 11 / Q000302:isolation & purification; D052162:Human papillomavirus 16 / Q000302:isolation & purification; D052163:Human papillomavirus 6 / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000821:virology*; D015995:Prevalence; D012189:Retrospective Studies; D065310:Squamous Intraepithelial Lesions of the Cervix / Q000453:epidemiology* / Q000473:pathology / Q000821:virology*; D055815:Young Adult
chemical_list: D004279:DNA, Viral
keywords: Atypical squamous cells of undetermined significance (ASCUS); Cervical cancer; Cytology; Human papillomavirus (HPV)