title: In-house development of an optimized synthetic module for routine [11C]acetate production.
abstract: [11C]Acetate, a radiotracer for PET imaging, is a promising radiopharmaceutical for overcoming the limitation of 2-deoxy-2-[18F]fluoro-D-glucose in a number of cancers. Here, the optimized automatic synthesis of [11C]acetate using an in-house-developed module under different conditions has been reported for routine production. [11C]CO2 was produced in a 16.4 MeV PETtrace cyclotron, and methyl magnesium chloride was used for synthesis. For product purification, ion-exchange solid-phase extraction cartridges were used, connected in series. High-performance liquid chromatography and gas chromatography were used to measure radiochemical and chemical purity. The Limulus amebocyte lysate test and the fluid thioglycollate medium test were performed for quality control of [11C]acetate. The total reaction time of [11C]acetate was within 15 min, and the overall decay-corrected radiochemical yield was 84.33±8.85%. Radiochemical purity was greater than 98% when evaluated on an analytical high-performance liquid chromatography system. No endotoxins or anaerobic bacteria were seen on quality control checks. Optimized production of [11C]acetate was achieved by the in-house module. Radiochemical and biological properties of the [11C]acetate produced were appropriate for clinical PET study.
authors.affiliation: aDepartment of Nuclear Medicine, Chonnam National University Hwasun Hospital, Hwasun bDepartment of Chemistry, Chonnam National University, Gwangju cRadiation Instrumentation Research Division, Korea Atomic Energy Research Institute, Jeongeup dDepartment of Advanced Materials Chemistry, College of Sciences & Technology, Dongguk University-GyeongJu, GyeongJu, Republic of Korea.
authors.forename: Hwa Youn
authors.initials: HY
authors.lastname: Jang
authors.forename: Seong Young
authors.initials: SY
authors.lastname: Kwon
authors.forename: Ayoung
authors.initials: A
authors.lastname: Pyo
authors.forename: Min Goo
authors.initials: MG
authors.lastname: Hur
authors.forename: Sang Wook
authors.initials: SW
authors.lastname: Kim
authors.forename: Jeong-Hoon
authors.initials: JH
authors.lastname: Park
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Seung Dae
authors.initials: SD
authors.lastname: Yang
authors.forename: Sunwoo
authors.initials: S
authors.lastname: Lee
authors.forename: Dong-Yeon
authors.initials: DY
authors.lastname: Kim
authors.forename: Jung-Joon
authors.initials: JJ
authors.lastname: Min
mesh_terms: D000085:Acetates / Q000138:chemical synthesis* / Q000737:chemistry; D002250:Carbon Radioisotopes; D060326:Chemistry Techniques, Synthetic; D006801:Humans; D049268:Positron-Emission Tomography; D011854:Radiochemistry / Q000379:methods*; D015203:Reproducibility of Results; D014057:Tomography, X-Ray Computed
chemical_list: D000085:Acetates; D002250:Carbon Radioisotopes