title: Paper-based electrochemical cyto-device for sensitive detection of cancer cells and in situ anticancer drug screening.
abstract: In this work, using human acute promyelocytic leukemia cells (HL-60) as a model, a novel microfluidic paper-based electrochemical cyto-device (μ-PECD) was fabricated to demonstrate a facile, portable, and disposable approach for cancer cell detection and in situ screening of anticancer drugs in a high-throughput manner. In this μ-PECD, aptamers modified three-dimensional macroporous Au-paper electrode (Au-PE) was fabricated and employed as the working electrode for specific and efficient cancer cell capture as well as for sequential in-electrode 3D cell culture. This Au-PE showed enhanced capture capacity for cancer cells and good biocompatibility for preserving the activity of captured living cells. Sensitive cancer cell detection was achieved in this μ-PECD, which could respond down to four HL-60 cells in 10 μL volume with a wide linear calibration range from 5.0×10(2) to 7.5×10(7) cells mL(-1) and exhibited good stability and reproducibility. Then, in situ anticancer drug screening was successfully implemented in this μ-PECD through monitoring of the apoptotic cancer cells after the in-electrode 3D cell culture with drug-containing culture medium, demonstrating its wide range of potential applications to facilitate effective clinical cancer diagnosis and treatment.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Min
authors.initials: M
authors.lastname: Su
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ge
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Shenguang
authors.initials: S
authors.lastname: Ge
authors.affiliation: School of Information Science and Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Nianqiang
authors.initials: N
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China. Electronic address: ujn.yujh@gmail.com.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yan
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Jiadong
authors.initials: J
authors.lastname: Huang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D015374:Biosensing Techniques / Q000295:instrumentation; D018929:Cell Culture Techniques / Q000295:instrumentation; D002469:Cell Separation / Q000295:instrumentation; D004354:Drug Screening Assays, Antitumor / Q000295:instrumentation*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D018922:HL-60 Cells; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010209:Paper; D015203:Reproducibility of Results
chemical_list: D000970:Antineoplastic Agents; D006046:Gold
keywords: Cancer cell; Cytosensor; Drug screening; Electrochemistry; Lab-on-paper