title: [Genetic diversity and phylogeny of soybean rhizobia isolated from the Hilly area of Central Sichuan in China].
abstract: OBJECTIVE
  We investigated the genetic diversity and phylogeny of 28 rhizobial isolates from root nodules of soybean growing in the Hilly Area of Central Sichuan in China.
  
  
  METHODS
  We used 16S rDNA PCR-RFLP and phylogenetic analyses of the 16S rDNA, glnII and symbiotic genes (nodC).
  
  
  RESULTS
  Five 16S rDNA genotypes among the isolates were distinguished with restriction endonucleases Hae III, Hinf I, Msp I and Taq I. In the 16S rDNA PCR-RFLP analysis, all the isolates are divided into Bradyrhizobium group and Sinonrhizobium group at the 83% level, and Sinonrhizobium strains accounted for 75% of the isolates. The phylogenetic analyses of 16S rDNA, glnII and nodC show that 4 representative strains SCAUs1, SCAUs2, SCAUs7 and SCAUs4 were closely related to S. fredii USDA205(T) while the other 2 representative strains SCAUs3 and SCAUs5 were closely related to B. yuanmingense CCBAU10071(T) and B. diazoefficiens USDA110(T). The 16S rDNA, glnII and nodC sequence similarity of 4 Sinonrhizobium representative strains were 98.3% - 99.9%, 98.2% - 100% and 100%, respectively.
  
  
  CONCLUSIONS
  Soybean rhizobia isolated from the Hilly Area of Central Sichuan in China has rich genetic diversity, S. fredii was the predominant genus.
authors.forename: Feng
authors.initials: F
authors.lastname: Xiong
authors.forename: Yuanxue
authors.initials: Y
authors.lastname: Chen
authors.forename: Silan
authors.initials: S
authors.lastname: Zhang
authors.forename: Fang
authors.initials: F
authors.lastname: You
authors.forename: Huan
authors.initials: H
authors.lastname: Zhou
authors.forename: Kaiwei
authors.initials: K
authors.lastname: Xu
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D002681:China; D007887:Fabaceae / Q000382:microbiology*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D012231:Rhizobium