title: Piperlonguminine is neuroprotective in experimental rat stroke.
abstract: Inflammatory damage plays an important role in cerebral ischemic pathogenesis and may represent a target for treatment. Piperlonguminine (PE) has been proved to have anti-inflammatory actions. In this study, we investigated the effects of PE on cultured neuronal cell line, SH-SY5Y in vitro and experimental rat ischemic stroke in vivo. For oxygen-glucose deprivation (OGD) and tumor necrosis factor-α (TNF-α) stimulated SH-SY5Y cell line in vitro, SH-SY5Y cells were incubated with PE. In vivo, rats were subjected to middle cerebral artery occlusion (MACO) for 1h, followed by reperfusion for 23 h. The results of this study showed that treatment of SH-SY5Y cells with PE reduced the OGD-induced cytotoxicity and apoptosis and blocked TNF-α-induced activation of NF-κB and MAPK. Intraperitoneal injection of PE (2.4 mg/kg) produced a significant neuroprotective potential in rats with cerebral ischemia. PE attenuated neurological deficit scores, brain infarct volume and brain water content in rats, and inhibited activation of NF-κB and MAPK. These data show that PE protects the brain against ischemic cerebral injury via alleviating blood-brain barrier (BBB) breakdown, which may be mediated via inhibiting NF-κB and MAPK signaling pathways.
authors.affiliation: First Affiliated Hospital, Heilongjiang University of Chinese Medicine, PR China.
authors.forename: Tiansong
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of Physiology, Heilongjiang University of Chinese Medicine, PR China.
authors.forename: Shixiao
authors.initials: S
authors.lastname: Sun
authors.affiliation: The First Affiliated Hospital, Harbin Medical University, Harbin, PR China.
authors.forename: Tiegang
authors.initials: T
authors.lastname: Wang
authors.affiliation: Atlantic Institute of Oriental Medicine (ATOM), FL, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Tong
authors.affiliation: Department of Formulas of Chinese Medicine, Heilongjiang University of Chinese Medicine, PR China.
authors.forename: Junhui
authors.initials: J
authors.lastname: Bi
authors.affiliation: First Affiliated Hospital, Heilongjiang University of Chinese Medicine, PR China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: First Affiliated Hospital, Heilongjiang University of Chinese Medicine, PR China; Department of Acupuncture, Heilongjiang University of Chinese Medicine, PR China. Electronic address: sunzhong_ren@163.com.
authors.forename: Zhongren
authors.initials: Z
authors.lastname: Sun
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000473:pathology; D015153:Blotting, Western; D002460:Cell Line; D002470:Cell Survival; D004148:Dioxolanes / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D008297:Male; D016328:NF-kappa B; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000188:drug therapy*; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D010881:Piperidones
chemical_list: D004148:Dioxolanes; D005947:Glucose; D016328:NF-kappa B; D018696:Neuroprotective Agents; D010100:Oxygen; D048051:p38 Mitogen-Activated Protein Kinases; C498077:piperlongumine; D010881:Piperidones
keywords: Experimental stroke; Piperlonguminine; Rats; Signaling pathways