title: Genotyping of the Duffy blood group among Plasmodium knowlesi-infected patients in Malaysia.
abstract: The Duffy blood group is of major interest in clinical medicine as it plays an important role in Plasmodium knowlesi and Plasmodium vivax infection. In the present study, the distribution of Duffy blood group genotypes and allelic frequencies among P. knowlesi infected patients as well as healthy individuals in Peninsular Malaysia were determined. The blood group of 60 healthy blood donors and 51 P. knowlesi malaria patients were genotyped using allele specific polymerase chain reaction (ASP-PCR). The data was analyzed using Fisher's exact test in order to assess the significance of the variables. Our results show a high proportion of the FY*A/FY*A genotype (>85% for both groups) and a high frequency of the FY*A allele (>90% for both groups). The FY*A/FY*A genotype was the most predominant genotype in both infected and healthy blood samples. The genotype frequency did not differ significantly between the donor blood and the malaria patient groups. Also, there was no significant correlation between susceptibility to P. knowlesi infection with any Duffy blood genotype.
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Jeremy Ryan
authors.initials: JR
authors.lastname: De Silva
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Tropical Disease Research and Education Centre, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Yee Ling
authors.initials: YL
authors.lastname: Lau
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Tropical Disease Research and Education Centre, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Mun Yik
authors.initials: MY
authors.lastname: Fong
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D004375:Duffy Blood-Group System / Q000145:classification / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D008288:Malaria / Q000175:diagnosis / Q000235:genetics* / Q000469:parasitology; D008296:Malaysia; D016790:Plasmodium knowlesi / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction
chemical_list: D004375:Duffy Blood-Group System