title: Structural and conformational study of the O-polysaccharide produced by the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris strain BisA53.
abstract: Rhodopseudomonas palustris is a purple photosynthetic bacterium characterized by a versatile nature and a remarkable ability to adapt to various environments. In this work, we focused our attention to its membrane characteristics and defined the structural and conformational features of the O-chain polysaccharide of LPS isolated from R. palustris strain BisA53. This strain produces a polymer with a trisaccharide repeating unit characterized by d-rhamnose, 3-deoxy-d-lyxo-2-heptulosaric acid (Dha), and a novel C-branched monosaccharide, a 4-amino-4,6-dideoxy-3-C-methyl-2-O-methyl-α-l-glucopyranose whose absolute configuration has been determined by a combination of 2D NMR spectroscopy and molecular mechanic and dynamic simulation.
authors.affiliation: Dipartimento di Scienze Chimiche, Complesso Universitario Monte Sant'Angelo, Università di Napoli Federico II, Via Cintia 4, I-80126 Napoli, Italy. Electronic address: silipo@unina.it.
authors.forename: Alba
authors.initials: A
authors.lastname: Silipo
authors.affiliation: Dipartimento di Scienze Chimiche, Complesso Universitario Monte Sant'Angelo, Università di Napoli Federico II, Via Cintia 4, I-80126 Napoli, Italy.
authors.forename: Flaviana
authors.initials: F
authors.lastname: Di Lorenzo
authors.affiliation: Dipartimento di Scienze Chimiche, Complesso Universitario Monte Sant'Angelo, Università di Napoli Federico II, Via Cintia 4, I-80126 Napoli, Italy.
authors.forename: Antonia
authors.initials: A
authors.lastname: De Felice
authors.affiliation: Dipartimento di Scienze Chimiche, Complesso Universitario Monte Sant'Angelo, Università di Napoli Federico II, Via Cintia 4, I-80126 Napoli, Italy.
authors.forename: Adele
authors.initials: A
authors.lastname: Vanacore
authors.affiliation: Dipartimento di Scienze Chimiche, Complesso Universitario Monte Sant'Angelo, Università di Napoli Federico II, Via Cintia 4, I-80126 Napoli, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: De Castro
authors.affiliation: IRD, Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM), UMR IRD/SupAgro/INRA/UM2/CIRAD, TA-A82/J - Campus de Baillarguet, 34398 Montpellier Cedex 5, France.
authors.forename: Djamel
authors.initials: D
authors.lastname: Gully
authors.affiliation: Dipartimento di Scienze Chimiche, Complesso Universitario Monte Sant'Angelo, Università di Napoli Federico II, Via Cintia 4, I-80126 Napoli, Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Lanzetta
authors.affiliation: Dipartimento di Scienze Chimiche, Complesso Universitario Monte Sant'Angelo, Università di Napoli Federico II, Via Cintia 4, I-80126 Napoli, Italy.
authors.forename: Michelangelo
authors.initials: M
authors.lastname: Parrilli
authors.affiliation: IRD, Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM), UMR IRD/SupAgro/INRA/UM2/CIRAD, TA-A82/J - Campus de Baillarguet, 34398 Montpellier Cedex 5, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Giraud
authors.affiliation: Dipartimento di Scienze Chimiche, Complesso Universitario Monte Sant'Angelo, Università di Napoli Federico II, Via Cintia 4, I-80126 Napoli, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000737:chemistry*; D012241:Rhodopseudomonas / Q000737:chemistry*
chemical_list: D011134:Polysaccharides
keywords: C-branched sugar; Lipopolysaccharide; Molecular modeling; NMR spectroscopy; Rhodopseudomonas palustris