title: The Gos28 SNARE protein mediates intra-Golgi transport of rhodopsin and is required for photoreceptor survival.
abstract: SNARE proteins play indispensable roles in membrane fusion events in many cellular processes, including synaptic transmission and protein trafficking. Here, we characterize the Golgi SNARE protein, Gos28, and its role in rhodopsin (Rh1) transport through Drosophila photoreceptors. Mutations in gos28 lead to defective Rh1 trafficking and retinal degeneration. We have pinpointed a role for Gos28 in the intra-Golgi transport of Rh1, downstream from α-mannosidase-II in the medial- Golgi. We have confirmed the necessity of key residues in Gos28's SNARE motif and demonstrate that its transmembrane domain is not required for vesicle fusion, consistent with Gos28 functioning as a t-SNARE for Rh1 transport. Finally, we show that human Gos28 rescues both the Rh1 trafficking defects and retinal degeneration in Drosophila gos28 mutants, demonstrating the functional conservation of these proteins. Our results identify Gos28 as an essential SNARE protein in Drosophila photoreceptors and provide mechanistic insights into the role of SNAREs in neurodegenerative disease.
authors.affiliation: From the Department of Ophthalmology and Visual Sciences, Department of Genetics and The McPherson Eye Research Institute, University of Wisconsin, Madison, Wisconsin 53792.
authors.forename: Erica E
authors.initials: EE
authors.lastname: Rosenbaum
authors.affiliation: From the Department of Ophthalmology and Visual Sciences, Department of Genetics and The McPherson Eye Research Institute, University of Wisconsin, Madison, Wisconsin 53792.
authors.forename: Eva
authors.initials: E
authors.lastname: Vasiljevic
authors.affiliation: From the Department of Ophthalmology and Visual Sciences, Department of Genetics and The McPherson Eye Research Institute, University of Wisconsin, Madison, Wisconsin 53792.
authors.forename: Spencer C
authors.initials: SC
authors.lastname: Cleland
authors.affiliation: From the Department of Ophthalmology and Visual Sciences, Department of Genetics and The McPherson Eye Research Institute, University of Wisconsin, Madison, Wisconsin 53792.
authors.forename: Carlos
authors.initials: C
authors.lastname: Flores
authors.affiliation: From the Department of Ophthalmology and Visual Sciences, Department of Genetics and The McPherson Eye Research Institute, University of Wisconsin, Madison, Wisconsin 53792 njcolley@wisc.edu.
authors.forename: Nansi Jo
authors.initials: NJ
authors.lastname: Colley
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001692:Biological Transport / Q000235:genetics; D015153:Blotting, Western; D002470:Cell Survival / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism*; D006801:Humans; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009154:Mutation; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism*; D050766:Qb-SNARE Proteins / Q000235:genetics / Q000378:metabolism*; D012162:Retinal Degeneration / Q000235:genetics / Q000378:metabolism; D012243:Rhodopsin / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
chemical_list: D029721:Drosophila Proteins; C000595189:Gos28 protein, Drosophila; D050766:Qb-SNARE Proteins; D012243:Rhodopsin
keywords: Drosophila; Golgi; Gos28; Neurodegeneration; Photoreceptor; Protein Trafficking; Retina; Retinal Degeneration; Rhodopsin; SNARE Proteins