title: Effects of chronic administration of tamsulosin and tadalafil, alone or in combination, in rats with bladder outlet obstruction induced by chronic nitric oxide deficiency.
abstract: OBJECTIVE
  The aim of this study was to define if tadalafil causes detrusor muscle impairment and to observe the effect of combination of tadalafil with tamsulosin on the lower urinary tract of rats with bladder outlet obstruction (BOO) induced by chronic nitric oxide deficiency.
  
  
  METHODS
  Thirty-one male rats were randomized to following groups: 1 - control; 2 - L-Nitroarginine methyl ester (L-NAME); 3 - Tamsulosin + L-NAME, 4 Tadalafil+L-NAME; and 5 - Tamsulosin + Tadalafil + L-NAME. At the end of the treatment period (30 days), all animals were submitted to urodynamic study.
  
  
  RESULTS
  The administration of L-NAME increased the number of non-voiding contractions (NVC) (1.04 ± 0.22), volume threshold (VT) (1.86 ± 0.35), and micturition cycle (MC) (1.34 ± 0.11) compared with control (0.52 ± 0.06, 0.62 ± 0.06, and 0.67 ± 0.30), respectively. The administration of tamsulosin reduced the number of NVC (0.57 ± 0.42) and VT (0.76 ± 0.24 ) compared with L-NAME group. Co-treatment with tadalafil decreased the number of VT (0.85 ± 0.53) and MC (0.76 ± 0.22) compared with L-NAME group. The combination of tamsulosin with tadalafil improved the number of NVC (0.56 ± 0.18), VT (0.97 ± 0.52) and MC (0.68 ± 0.30) compared with L-NAME group.
  
  
  CONCLUSIONS
  In rats with BOO induced by chronic nitric oxide deficiency, tadalafil did not cause impairment in detrusor muscle and seems to have an addictive effect to tamsulosin because the combination decreased non voiding contractions as well the number of micturition cycles.
authors.affiliation: Division of Urology, Universidade Federal do Ceara, CE, Brazil.
authors.forename: Rommel Prata
authors.initials: RP
authors.lastname: Regadas
authors.affiliation: Division of Urology, Universidade Federal do Ceara, CE, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Reges
authors.affiliation: Division of Urology, Universidade Federal do Ceara, CE, Brazil.
authors.forename: João Batista Gadelha
authors.initials: JB
authors.lastname: Cerqueira
authors.affiliation: Division of Urology, Universidade Federal do Ceara, CE, Brazil.
authors.forename: Daniel Gabrielle
authors.initials: DG
authors.lastname: Sucupira
authors.affiliation: Department of Pharmacology, Universidade Federal do Ceara, CE, Brazil.
authors.forename: Francisco Vagnaldo F
authors.initials: FV
authors.lastname: Jamacaru
authors.affiliation: Department of Pharmacology, Universidade Federal do Ceara, CE, Brazil.
authors.forename: Manoel Odorico de
authors.initials: MO
authors.lastname: Moraes
authors.affiliation: Division of Urology, Universidade Federal do Ceara, CE, Brazil.
authors.forename: Lúcio Flávio
authors.initials: LF
authors.lastname: Gonzaga-Silva
mesh_terms: D000818:Animals; D002243:Carbolines / Q000008:administration & dosage*; D004359:Drug Therapy, Combination; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000008:administration & dosage; D009569:Nitric Oxide / Q000172:deficiency; D058986:Phosphodiesterase 5 Inhibitors / Q000008:administration & dosage; D011897:Random Allocation; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013449:Sulfonamides / Q000008:administration & dosage*; D000068581:Tadalafil; D000077409:Tamsulosin; D016896:Treatment Outcome; D001748:Urinary Bladder Neck Obstruction / Q000188:drug therapy* / Q000209:etiology; D014554:Urination / Q000187:drug effects; D064804:Urological Agents / Q000008:administration & dosage*
chemical_list: D002243:Carbolines; D058986:Phosphodiesterase 5 Inhibitors; D013449:Sulfonamides; D064804:Urological Agents; D009569:Nitric Oxide; D000068581:Tadalafil; D000077409:Tamsulosin; D019331:NG-Nitroarginine Methyl Ester