title: Comprehensive large-scale assessment of intrinsic protein disorder.
abstract: BACKGROUND
  Intrinsically disordered regions are key for the function of numerous proteins. Due to the difficulties in experimental disorder characterization, many computational predictors have been developed with various disorder flavors. Their performance is generally measured on small sets mainly from experimentally solved structures, e.g. Protein Data Bank (PDB) chains. MobiDB has only recently started to collect disorder annotations from multiple experimental structures.
  
  
  RESULTS
  MobiDB annotates disorder for UniProt sequences, allowing us to conduct the first large-scale assessment of fast disorder predictors on 25 833 different sequences with X-ray crystallographic structures. In addition to a comprehensive ranking of predictors, this analysis produced the following interesting observations. (i) The predictors cluster according to their disorder definition, with a consensus giving more confidence. (ii) Previous assessments appear over-reliant on data annotated at the PDB chain level and performance is lower on entire UniProt sequences. (iii) Long disordered regions are harder to predict. (iv) Depending on the structural and functional types of the proteins, differences in prediction performance of up to 10% are observed.
  
  
  BACKGROUND
  The datasets are available from Web site at URL: http://mobidb.bio.unipd.it/lsd.
  
  
  BACKGROUND
  Supplementary data are available at Bioinformatics online.
authors.affiliation: Department of Biomedical Sciences, Department of Information Engineering, University of Padua, Via Gradenigo 6, 35121 Padova, Italy and Institute for Advanced Simulation, Forschungszentrum Juelich, Wilhelm-Johnen-Str., 52425 Juelich, Germany.
authors.forename: Ian
authors.initials: I
authors.lastname: Walsh
authors.affiliation: Department of Biomedical Sciences, Department of Information Engineering, University of Padua, Via Gradenigo 6, 35121 Padova, Italy and Institute for Advanced Simulation, Forschungszentrum Juelich, Wilhelm-Johnen-Str., 52425 Juelich, Germany Department of Biomedical Sciences, Department of Information Engineering, University of Padua, Via Gradenigo 6, 35121 Padova, Italy and Institute for Advanced Simulation, Forschungszentrum Juelich, Wilhelm-Johnen-Str., 52425 Juelich, Germany.
authors.forename: Manuel
authors.initials: M
authors.lastname: Giollo
authors.affiliation: Department of Biomedical Sciences, Department of Information Engineering, University of Padua, Via Gradenigo 6, 35121 Padova, Italy and Institute for Advanced Simulation, Forschungszentrum Juelich, Wilhelm-Johnen-Str., 52425 Juelich, Germany.
authors.forename: Tomás
authors.initials: T
authors.lastname: Di Domenico
authors.affiliation: Department of Biomedical Sciences, Department of Information Engineering, University of Padua, Via Gradenigo 6, 35121 Padova, Italy and Institute for Advanced Simulation, Forschungszentrum Juelich, Wilhelm-Johnen-Str., 52425 Juelich, Germany.
authors.forename: Carlo
authors.initials: C
authors.lastname: Ferrari
authors.affiliation: Department of Biomedical Sciences, Department of Information Engineering, University of Padua, Via Gradenigo 6, 35121 Padova, Italy and Institute for Advanced Simulation, Forschungszentrum Juelich, Wilhelm-Johnen-Str., 52425 Juelich, Germany.
authors.forename: Olav
authors.initials: O
authors.lastname: Zimmermann
authors.affiliation: Department of Biomedical Sciences, Department of Information Engineering, University of Padua, Via Gradenigo 6, 35121 Padova, Italy and Institute for Advanced Simulation, Forschungszentrum Juelich, Wilhelm-Johnen-Str., 52425 Juelich, Germany.
authors.forename: Silvio C E
authors.initials: SC
authors.lastname: Tosatto
mesh_terms: D018360:Crystallography, X-Ray; D030562:Databases, Protein; D006801:Humans; D058977:Molecular Sequence Annotation; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D020539:Sequence Analysis, Protein / Q000379:methods*; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry*
chemical_list: D011506:Proteins; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53