title: [Effect of parathyroid hormone (1-34) on bone metastasis of lung cancer cells in mice].
abstract: OBJECTIVE
  To investigate the effect of parathyroid hormone (1-34) (PTH) on tumor growth in a mouse model of lung cancer with bone metastasis.
  
  
  METHODS
  Mouse models of proximal tibial bone metastasis of lung cancer were established in 30 female BALB%c mice. The mouse models were randomly divided into 3 groups and received injections with 40 mg%kg PTH (1-34), equal amount of solvent (blank control), or cyclophosphamide (positive control). Body weight of the mice was measured every 2 days and the right hind limb tumor growth was observed. The mice were sacrificed after 28 days for X-ray and CT examinations to observe the tumor shape, size, tibial bone density, and tumor volume. HE staining and immunohistochemistry were performed to observe the tumor morphology and pathological type, and serum concentration of serum alkaline phosphatase (ALP) was detected.
  
  
  RESULTS
  The body weight change curves did not show significant difference between PTH (1-34) group and the blank control group (P>0.05). In both PTH (1-34) group and the blank control group, X-ray and micro-CT revealed significant bone defects, and in cyclophosphamide group the bone cortex was basically intact with only mild bone destruction. The tumor volume was similar between PTH (1-34) group and the blank control group (P>0.05), but significantly smaller in cyclophosphamide group (P<0.05). The bone density in PTH (1-34) group was significantly greater than that in the blank control group, but lower than that in cyclophosphamide group (P<0.05). Pathological examination revealed mainly osteolytic lesions mixed with bone destruction, which was severer in PTH (1-34) group and blank control group with obvious tumor cell filling of the defects; immunohistochemistry identified the tumors as adenomas. ALP activity was higher in PTH (1-34) group than in the other two group and differed significantly between the 3 groups (P<0.05).
  
  
  CONCLUSIONS
  Intermittent small-dose injections of parathyroid hormone PTH (1-34) does not promote bone metastatic tumor growth in mice and increases the bone quantity around the metastatic lesions.
authors.affiliation: 1Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; 2Lecong Hospital, Foshan 528000, China. E-mail: 31365293@qq.com.
authors.forename: Chunyu
authors.initials: C
authors.lastname: Li
authors.forename: Dehong
authors.initials: D
authors.lastname: Yang
authors.forename: Yue
authors.initials: Y
authors.lastname: Meng
authors.forename: Song
authors.initials: S
authors.lastname: Hao
mesh_terms: D000818:Animals; D015519:Bone Density; D001859:Bone Neoplasms / Q000556:secondary*; D004195:Disease Models, Animal; D005260:Female; D008175:Lung Neoplasms / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010281:Parathyroid Hormone / Q000494:pharmacology*; D013977:Tibia / Q000473:pathology
chemical_list: D010281:Parathyroid Hormone