title: Improvement of sugar analysis sensitivity using anion-exchange chromatography-electrospray ionization mass spectrometry with sheath liquid interface.
abstract: A novel interface that enables high-performance anion-exchange chromatography (HPAEC) to be coupled with electrospray ionization (ESI) mass spectrometry (MS) is reported. A sheath liquid consisting of 50mM NH4Ac in isopropanol with 0.05% acetic acid, infused at a flow rate of 3μL/min at the tip of the electrospray probe, requires less ESI source cleaning and promotes efficient ionization of mono- and di-carbohydrates. The results suggest that use of a sheath liquid interface rather than a T-joint allows volatile ammonium salts to replace non-volatile metal salts as modifiers for improving sugar ESI signals. The efficient ionization of mono- and di-carbohydrates in the ESI source is affected by the sheath liquid properties such as buffer concentration and type of organic solvent. HPAEC-ESI-MS was used for the analysis of monocarbohydrates in pectins, particularly co-eluted sugars, and the performance was evaluated. Addition of a make-up solution through the sheath liquid interface proved to be an efficient tool for enhancing the intensities of sugars analyzed using HPAEC-ESI-MS.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Xian-Bing
authors.initials: XB
authors.lastname: Xu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Ding-Bo
authors.initials: DB
authors.lastname: Liu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Xiao Ming
authors.initials: XM
authors.lastname: Guo
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China; State Key Laboratory of Pulp and Paper Engineering, Guangzhou 510640, China. Electronic address: shujuanyu8@gmail.com.
authors.forename: Shu-Juan
authors.initials: SJ
authors.lastname: Yu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Yu
mesh_terms: D000838:Anions / Q000737:chemistry; D002021:Buffers; D002241:Carbohydrates / Q000032:analysis*; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000838:Anions; D002021:Buffers; D002241:Carbohydrates
keywords: Carbohydrate; HPAEC-ESI-MS; Ionic cluster; Jerusalem artichoke; Pectin; Sheath liquid