title: Selected reaction monitoring assays in mesenchymal stem cells from osteoarthritis patients.
abstract: Osteoarthritis (OA) is considered the most prevalent form of arthritis. The aim of this study was to verify potential protein OA biomarkers by applying Selected Reaction Monitoring (SRM) assays to protein extracts obtained from Bone Marrow-Mesenchymal Stem Cells (BM-MSCs) isolated from OA patients. BM aspirates were obtained from the femoral channel of OA patients at the time of surgery and from the femoral channel of hip fracture subjects without OA during hip joint replacement surgery for the treatment of subcapital fracture. SRM results verified the differential expression of several protein biomarkers in BM-MSCs from OA patients.
authors.affiliation: Unidad de Proteómica, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain.
authors.forename: Emilio
authors.initials: E
authors.lastname: Camafeita
authors.affiliation: Rheumatology Service, Hospital Clínico San Carlos, Profesor Martín Lagos s/n, Madrid E-28040, Spain.
authors.forename: José-Ramón
authors.initials: JR
authors.lastname: Lamas
authors.affiliation: Unidad de Proteómica, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Calvo
authors.affiliation: Rheumatology Service, Hospital Clínico San Carlos, Profesor Martín Lagos s/n, Madrid E-28040, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Tornero-Esteban
authors.affiliation: Unidad de Proteómica, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain.
authors.forename: Juan-Antonio
authors.initials: JA
authors.lastname: López
authors.affiliation: Rheumatology Service, Hospital Clínico San Carlos, Profesor Martín Lagos s/n, Madrid E-28040, Spain.
authors.forename: Benjamín
authors.initials: B
authors.lastname: Fernández-Gutiérrez
keywords: Mesenchymal stem cells; Osteoarthritis; Selected reaction monitoring