title: [Shenkang pill down-regulates AOPP-induced expression of inflammatory factor MCP-1 via a p38MAPK/NF-κB-dependent mechanism].
abstract: OBJECTIVE
  To investigate the effect of the serum of rats fed with Shenkang pill in regulating monocyte chemoattractant protein 1 (MCP-1) expression induced by advanced oxidation protein products (AOPP) in mouse podocyte clone 5 (MPC5) and explore the underlying mechanism.
  
  
  METHODS
  MPC5 cultured in vitro were incubated for different time lengths in the presence of different concentrations of serum of rats medicated with Shenkang pill, and the cell proliferation was assessed using MTT assay. In MPC5 treated with AOPP prior to exposure to the rat serum, the changes in the protein expressions of p38MAPK and IκBα were examined with Western blotting, NF-κB p65 nuclear translocation was analyzed with immunofluorescence assay, and MCP-1 expression in the supernatant was determined using ELISA kits.
  
  
  RESULTS
  The medicated rat serum time- and concentration-dependently promoted the proliferation of MPC5, with the optimal serum concentration of 5% and incubation time of 24 h. AOPP significantly increased MCP-1 expression in the cell supernatant in a time-and concentration-dependent manner; pretreatment with SB203580 (a p38 inhibitor) or parthenolide (a NF-κB inhibitor) significantly decreased MCP-1 expression, and treatment with the medicated serum significantly decreased AOPP-induced MCP-1 expression. AOPP concentration-dependently increased the protein expression of P-p38 but decreased that of IκBα. Both the medicated serum and SB203580 increased IκBα protein in AOPP-induced cells, but the effect was more obvious with the medicated serum. The medicated serum also decreased NF-κB p65 nuclear translocation in AOPP-induced MPC5.
  
  
  CONCLUSIONS
  Shenkang pill-medicated serum can decrease AOPP-induced expression of MPC-1 in MPC5 by regulating p38MAPK/NF-κB to mediate its anti-inflammatory effect. This finding provides a new theoretical basis for the application of Shenkang pill to treat diabetic nephropathy.
authors.affiliation: Department of Nephrology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. E-mail: yan.er0306@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jiancheng
authors.initials: J
authors.lastname: Wang
authors.forename: Hongyu
authors.initials: H
authors.lastname: Li
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Jia
authors.forename: Sijia
authors.initials: S
authors.lastname: Chen
authors.forename: Zhaozhong
authors.initials: Z
authors.lastname: Xu
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Du
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Chen
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.forename: Bo
authors.initials: B
authors.lastname: Huang
authors.forename: Haibo
authors.initials: H
authors.lastname: Long
mesh_terms: D062107:Advanced Oxidation Protein Products / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D018932:Chemokine CCL2 / Q000378:metabolism*; D015536:Down-Regulation; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D020672:I-kappa B Proteins / Q000378:metabolism; D007093:Imidazoles; D051379:Mice; D000072000:NF-KappaB Inhibitor alpha; D011725:Pyridines; D051381:Rats; D044967:Serum / Q000737:chemistry; D012717:Sesquiterpenes; D051996:Transcription Factor RelA / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D062107:Advanced Oxidation Protein Products; C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D004365:Drugs, Chinese Herbal; D020672:I-kappa B Proteins; D007093:Imidazoles; C000606205:Nfkbia protein, mouse; C000606206:Nfkbia protein, rat; D011725:Pyridines; C498145:Rela protein, rat; D012717:Sesquiterpenes; D051996:Transcription Factor RelA; C550195:shenkang; D000072000:NF-KappaB Inhibitor alpha; C002669:parthenolide; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580