title: Classical and remote post-conditioning effects on ischemia/reperfusion-induced acute oxidant kidney injury.
abstract: The present study aimed to analyze and compare the effects of classical and remote ischemic postconditioning (POC) on rat renal ischemia/reperfusion (IR)-induced acute kidney injury. After right nephrectomy, male rats were randomly assigned into four groups (n = 8). In the IR group, 45 min of left renal artery occlusion was induced followed by 24 h of reperfusion. In the classical POC group, after induction of 45 min ischemia, 4 cycles of 10 s of intermittent ischemia and reperfusion were applied to the kidney before complete restoring of renal blood. In the remote POC group, 4 cycles of 5 min ischemia and reperfusion of left femoral artery were applied after 45 min renal ischemia and right at the time of renal reperfusion. There was a reduction in renal function (increase in blood urea and creatinine) in the IR group. Application of both forms of POC prevented the IR-induced reduction in renal function and histology. There were also significant improvements in kidney oxidative stress status in both POC groups demonstrated by a reduction in malondialdehyde (MDA) formation and preservation of antioxidant levels comparing to the IR group. We concluded that both methods of POC have protective effects on renal function and histology possibly by a reduction in IR-induced oxidative stress.
authors.affiliation: Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran 14155-6447, Iran. Electronic address: kadkhodm@tums.ac.ir.
authors.forename: Mehri
authors.initials: M
authors.lastname: Kadkhodaee
authors.affiliation: Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran 14155-6447, Iran.
authors.forename: Atefeh
authors.initials: A
authors.lastname: Najafi
authors.affiliation: Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran 14155-6447, Iran.
authors.forename: Behjat
authors.initials: B
authors.lastname: Seifi
mesh_terms: D058186:Acute Kidney Injury / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D003404:Creatinine / Q000378:metabolism; D004195:Disease Models, Animal; D057775:Ischemic Postconditioning; D007668:Kidney / Q000098:blood supply*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000150:complications* / Q000378:metabolism / Q000473:pathology
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D003404:Creatinine
keywords: Acute kidney injury; Classical post-conditioning; Ischemia-reperfusion; Remote post-conditioning