title: Time domain dielectric spectroscopy of nanosecond pulsed electric field induced changes in dielectric properties of pig whole blood.
abstract: The dielectric spectra of fresh pig whole blood in the β-dispersion range after exposure to 300-nanosecond pulsed electric fields (nsPEFs) with amplitude higher than the supra-electroporation threshold for erythrocytes were recorded by time domain reflectometry dielectric spectroscopy. The implications of the dielectric parameters on the dynamics of post-pulse pore development were discussed in light of the Cole-Cole relaxation model. The temporal development of the Cole-Cole parameters indicates that nsPEFs induced significant poration and swelling of erythrocytes within the first 5 min. The results also show that the majority of erythrocytes could not fully recover from supra-electroporation up to 30 min. The findings of this study suggest that time domain dielectric spectroscopy is a promising label-free and real-time physiological measuring technique for nsPEF-blood related biomedical applications, capable of following the conformational and morphological changes of cells.
authors.affiliation: Leibniz Institute for Plasma Science and Technology, Felix-Hausdorff-Strasse 2, 17489 Greifswald, Germany.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhuang
authors.affiliation: Leibniz Institute for Plasma Science and Technology, Felix-Hausdorff-Strasse 2, 17489 Greifswald, Germany. Electronic address: juergen.kolb@inp-greifswald.de.
authors.forename: Juergen F
authors.initials: JF
authors.lastname: Kolb
mesh_terms: D000818:Animals; D001769:Blood; D058266:Dielectric Spectroscopy / Q000379:methods*; D004558:Electric Stimulation; D018274:Electroporation; D004912:Erythrocytes / Q000737:chemistry* / Q000502:physiology; D034421:Sus scrofa; D013997:Time Factors
keywords: Electroporation; Nanosecond pulsed electric field; Time domain dielectric spectroscopy; Whole blood