title: Isolation and biological and molecular characterization of Toxoplasma gondii from canine cutaneous toxoplasmosis in Brazil.
abstract: Cutaneous toxoplasmosis is a rare manifestation. This study represents a case report of an immunosuppressed dog that developed nodular dermal lesions caused by Toxoplasma gondii. The isolate (TgDgBr20) was characterized as mouse virulent and was genotyped as type BrI (ToxoDB genotype 6) using PCR-restriction fragment length polymorphism (RFLP) and as Africa 1 through microsatellite analysis.
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil hfpena@usp.br.
authors.forename: Hilda F J
authors.initials: HF
authors.lastname: Pena
authors.affiliation: Departamento de Cirurgia Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Ludmila R
authors.initials: LR
authors.lastname: Moroz
authors.affiliation: Independent Researcher, São Paulo, Brazil.
authors.forename: Rita K B
authors.initials: RK
authors.lastname: Sozigan
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Daniel
authors.initials: D
authors.lastname: Ajzenberg
authors.affiliation: Laboratório de Imunoparasitologia Dr. Mario Endsfeldz Camargo, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil.
authors.forename: Fernando R
authors.initials: FR
authors.lastname: Carvalho
authors.affiliation: Laboratório de Imunoparasitologia Dr. Mario Endsfeldz Camargo, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil.
authors.forename: Caroline M
authors.initials: CM
authors.lastname: Mota
authors.affiliation: Laboratório de Imunoparasitologia Dr. Mario Endsfeldz Camargo, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil.
authors.forename: Tiago W P
authors.initials: TW
authors.lastname: Mineo
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Arlei
authors.initials: A
authors.lastname: Marcili
mesh_terms: D000818:Animals; D001938:Brazil; D016054:DNA, Protozoan / Q000235:genetics; D004195:Disease Models, Animal; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D005838:Genotype; D016867:Immunocompromised Host; D008297:Male; D051379:Mice; D018895:Microsatellite Repeats; D012150:Polymorphism, Restriction Fragment Length; D014122:Toxoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D014124:Toxoplasmosis, Animal / Q000469:parasitology*; D014774:Virulence
chemical_list: D016054:DNA, Protozoan