title: Btbd7 contributes to reduced E-cadherin expression and predicts poor prognosis in non-small cell lung cancer.
abstract: BACKGROUND
  Disorders of cell adhesion are critical steps in cancer progression in which varieties of markers including cadherins are involved in.Btbd7 was found to inhibit E-cadherin expression in MDCK cells and play important roles during branching morphogenesis of embryonic salivary glands and lungs. However its function in malignant tumors is largely unknown. The aim of this study is to investigate the clinicopathological significance and possible function of Btbd7 in non-small cell lung cancer.
  
  
  METHODS
  Immunohistochemistry and Western blotting were used to investigate Btbd7 expression in non-small cell lung cancer and lung tissues. The clinicopathological association and the overall survival was analyzed. In vitro experiments were performed using siRNA to investigate the function of Btbd7 in lung cancer cells.
  
  
  RESULTS
  Btbd7 expression was elevated in non-small cell lung cancer tissues compared to normal lung tissues. Increased Btbd7 expression was significantly associated with lymph node metastasis, reduced E-cadherin expression and patients' poor clinical outcome. Downregulation of Btbd7 expression in lung cancer cells by siRNA significantly inhibits cancer cell invasion and effectively restores E-cadherin expression in cancer cell membrane.
  
  
  CONCLUSIONS
  Btbd7 contributes to reduced expression of E-cadherin and may be a promising cancer marker in non-small cell lung cancer.
authors.forename: Chuifeng
authors.initials: C
authors.lastname: Fan
authors.forename: Yuan
authors.initials: Y
authors.lastname: Miao
authors.forename: Xiupeng
authors.initials: X
authors.lastname: Zhang
authors.forename: Di
authors.initials: D
authors.lastname: Liu
authors.forename: Guiyang
authors.initials: G
authors.lastname: Jiang
authors.forename: Xuyong
authors.initials: X
authors.lastname: Lin
authors.forename: Qiang
authors.initials: Q
authors.lastname: Han
authors.forename: Lan
authors.initials: L
authors.lastname: Luan
authors.forename: Zhonghai
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang 110001, China. wangeh@hotmail.com.
authors.forename: Enhua
authors.initials: E
authors.lastname: Wang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D015703:Antigens, CD; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000401:mortality / Q000556:secondary; D045744:Cell Line, Tumor; D002465:Cell Movement; D004285:Dogs; D005260:Female; D015870:Gene Expression; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D008207:Lymphatic Metastasis; D061985:Madin Darby Canine Kidney Cells; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D016016:Proportional Hazards Models
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015703:Antigens, CD; C436517:BTBD7 protein, human; D014408:Biomarkers, Tumor; C513384:CDH1 protein, human; D015820:Cadherins; D009363:Neoplasm Proteins