title: Surfactant protein A is decreased in the lung of rats with hepatopulmonary syndrome.
abstract: OBJECTIVE
  To evaluate surfactant protein A levels in an hepatopulmonary syndrome rat model. To date, there have been no studies aimed at evaluating surfactant levels in the setting of cirrhosis or hepatopulmonary syndrome.
  
  
  METHODS
  A total of 35 rats were divided into control, sham, and experimental HPS groups. We evaluated surfactant protein A levels in rats and the experimental model designed to induce hepatopulmonary syndrome was common bile duct ligation. Statistical analysis was performed using GraphPad Prism Software(r). Differences were considered statistically significant when p<0.05.
  
  
  RESULTS
  Lung homogenate of surfactant protein A levels were lower in the experimental hepatopulmonary syndrome and sham groups in comparison to the control group (p<0.05). Serum SP-A levels were the same in experimental hepatopulmonary syndrome and control groups but decreased in the sham group compared with the experimental groups (p<0.05). Myeloperoxidase activity was higher in the experimental hepatopulmonary syndrome group than the other two groups (p<0.05).
  
  
  CONCLUSIONS
  Surfactant protein A is present in experimental hepatopulmonary syndrome and leads to an imbalance between serum and pulmonary levels due to systemic inflammatory response.
authors.affiliation: School of Medicine, University of Sao Paulo, Brazil.
authors.forename: Lucas Souto
authors.initials: LS
authors.lastname: Nacif
authors.affiliation: Department of Gastroenterology, School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Wellington
authors.initials: W
authors.lastname: Andraus
authors.affiliation: Department of Gastroenterology, School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Márcia Saldanha
authors.initials: MS
authors.lastname: Kubrusly
authors.affiliation: University of São Paulo, Sao Paulo, SP, Brazil.
authors.forename: Flávia Saldanha
authors.initials: FS
authors.lastname: Kubrusly
authors.affiliation: University of São Paulo, Sao Paulo, SP, Brazil.
authors.forename: Vera Cristina Bugelli Cainelli
authors.initials: VC
authors.lastname: Gebara
authors.affiliation: Biotechnology Center, Butantan Institute, Sao Paulo, SP, Brazil.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ishizawa
authors.affiliation: Department of Gastroenterology, School of Medicine, USP, Sao Paulo, SP, Brazil.
authors.forename: Luiz Augusto Carneiro
authors.initials: LA
authors.lastname: D'Albuquerque
mesh_terms: D000818:Animals; D001784:Blood Gas Analysis; D003135:Common Bile Duct; D004195:Disease Models, Animal; D020065:Hepatopulmonary Syndrome / Q000378:metabolism* / Q000473:pathology; D008026:Ligation; D008168:Lung / Q000378:metabolism*; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D037662:Pulmonary Surfactant-Associated Protein A / Q000032:analysis / Q000378:metabolism*; D017208:Rats, Wistar; D012016:Reference Values
chemical_list: D037662:Pulmonary Surfactant-Associated Protein A; D009195:Peroxidase