title: Bacterial enzymes effectively digest Alzheimer's β-amyloid peptide.
abstract: Aggregated β-amyloid peptides play key roles in the development of Alzheimer's disease, and recent evidence suggests that microbial particles, among others, can facilitate their polymerization. Bacterial enzymes, however, have been proved to be beneficial in degrading pathological fibrillar structures in clinical settings, such as strepto-kinases in resolving blood-clots. The purpose of this study was to investigate the ability of bacterial substances to effectively hydrolyze β-amyloid peptides. Degrading products of several proteinases from Bacillus pumilus were evaluated using MALDI-TOF mass-spectrometry, and their toxicity was assessed in vitro using cell-culture assays and morphological studies. These enzymes have proved to be non-toxic and were demonstrated to cleave through the functional domains of β-amyloid peptide. By yielding inactive fragments, proteinases of Bacillus pumilus may be used as candidate anti-amyloid agents.
authors.affiliation: Kazan Federal University, Kazan, Russia.
authors.forename: Yuliya Vasilyevna
authors.initials: YV
authors.lastname: Danilova
authors.affiliation: Kazan Federal University, Kazan, Russia.
authors.forename: Elena Ilyasovna
authors.initials: EI
authors.lastname: Shagimardanova
authors.affiliation: Kazan Federal University, Kazan, Russia.
authors.forename: Anna Borisovna
authors.initials: AB
authors.lastname: Margulis
authors.affiliation: Kazan Federal University, Kazan, Russia.
authors.forename: Anna Aleksandrovna
authors.initials: AA
authors.lastname: Toymentseva
authors.affiliation: Kazan Federal University, Kazan, Russia.
authors.forename: Nelly Pavlovna
authors.initials: NP
authors.lastname: Balaban
authors.affiliation: Kazan Federal University, Kazan, Russia.
authors.forename: Nataliya Leonidovna
authors.initials: NL
authors.lastname: Rudakova
authors.affiliation: Kazan Federal University, Kazan, Russia.
authors.forename: Albert Anatolyevich
authors.initials: AA
authors.lastname: Rizvanov
authors.affiliation: Kazan Federal University, Kazan, Russia. Electronic address: margarita.sharipova@kpfu.ru.
authors.forename: Margarita Rashidovna
authors.initials: MR
authors.lastname: Sharipova
authors.affiliation: Kazan Federal University, Kazan, Russia; Asklepios-Med (Private Medical Practice and Research Center), Szeged, Hungary. Electronic address: palotas@asklepios-med.eu.
authors.forename: András
authors.initials: A
authors.lastname: Palotás
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D001407:Bacillus / Q000201:enzymology*; D006801:Humans; D009447:Neuroblastoma; D010446:Peptide Fragments / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D059748:Proteolysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014407:Tumor Cells, Cultured
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D010447:Peptide Hydrolases
keywords: Aggregation; Alzheimer's disease; Bacillus pumilus; Bacterium; Proteinase; β-Amyloid-peptide