title: Genetic characterization and pathogenicity assessment of Newcastle disease virus isolated from wild peacock.
abstract: The continued spread and occurrence of Newcastle disease virus (NDV) has posed potential threat to domestic poultry industry around the globe. Mainly, wild avian species has always been implicated for the natural reservoir for virus and spread of the disease. In the present study, we report the isolation of Newcastle disease virus (NDV/Peacock/India/2012) in necropsy brain tissue sample of wild peacock from North India. Complete genome of the virus was found to be 15,186 nucleotides (nts) with six genes in order of 3'-N-P-M-F-HN-L-5', which was limited by 55-nts leader region at the 3' end and a 114-nts trailer sequence at 5' end. Sequence analysis of fusion protein revealed the dibasic amino acid cleavage site (112)R-R-Q-K-R-F(117), a characteristic motif of virulent virus. Phylogenetic analysis placed the isolate in genotype II of Newcastle disease virus showing the lowest mean percent divergence (6 %) with other genotype II counterparts. The isolate was characterized as mesogenic (intermediate pathotype) based on the mean death time (63 h) in embryonated chicken eggs and the intra-cerebral pathogenicity index (1.40) in day-old chicks. The report emphasizes the dynamic ecology of NDV strains circulating in a wild avian host during the outbreak of 2012 in North India. Further the genotypic and pathotypical characterizations of the isolate could help in development of homologous vaccine against NDV strain circulating in avian population.
authors.affiliation: Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
authors.forename: Sagar A
authors.initials: SA
authors.lastname: Khulape
authors.forename: Satish S
authors.initials: SS
authors.lastname: Gaikwad
authors.forename: Madhan Mohan
authors.initials: MM
authors.lastname: Chellappa
authors.forename: Bishnu Prasad
authors.initials: BP
authors.lastname: Mishra
authors.forename: Sohini
authors.initials: S
authors.lastname: Dey
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000821:virology; D001715:Bird Diseases / Q000821:virology*; D001921:Brain / Q000821:virology; D002642:Chick Embryo; D016000:Cluster Analysis; D046094:Galliformes / Q000821:virology*; D016679:Genome, Viral; D005838:Genotype; D007194:India; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000821:virology*; D009522:Newcastle disease virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D016019:Survival Analysis
chemical_list: D012367:RNA, Viral