title: MWCNT-cysteamine-Nafion modified gold electrode based on myoglobin for determination of hydrogen peroxide and nitrite.
abstract: In this work, a novel amperometric biosensor of hydrogen peroxide (H2O2) was developed based on the immobilization of myoglobin (Mb) on the surface of the multi-walled carbon nanotube (MWCNT) -Nafion-cysteamine (CA) modified gold electrode (Au) and its electrocatalytic activity was used for the determination of nitrite (NO2(-)). In the optimization studies, the best MWCNT and myoglobin amount were investigated. It was discovered at the experiments for the optimization of the working conditions that the buffer at this study as 50.0mM, pH7.0 phosphate buffer (PBS) and working temperature as 30°C for the H2O2 biosensor. It was determined at the characterization studies on the biosensor that linear results are obtained between the ranges of 0.1μM to 70.0μM for H2O2 concentration and 1-250μM for NO2(-). The reproducibility of the biosensor was determined both H2O2 and nitrite. From the experiments, average value, standard deviation (SD) and coefficients of variation (CV%) were calculated to be 10.02±0.43μM, and 4.29% for 10.0μM H2O2 (n=6) and 52.0±2.1μM, and 3.89% for 50.0μM nitrite (n=8), respectively. At the same time the sample was analyzed for NO2(-) in drinking and mineral waters.
authors.affiliation: Department of Biochemistry, Faculty of Science, Ege University, 35100 Bornova, Izmir, Turkey. Electronic address: erhancanbay87@gmail.com.
authors.forename: Erhan
authors.initials: E
authors.lastname: Canbay
authors.affiliation: Ozel Ege High School, 35050 Bornova, Izmir, Turkey.
authors.forename: Berika
authors.initials: B
authors.lastname: Şahin
authors.affiliation: Ozel Ege High School, 35050 Bornova, Izmir, Turkey.
authors.forename: Müge
authors.initials: M
authors.lastname: Kıran
authors.affiliation: Department of Biochemistry, Faculty of Science, Ege University, 35100 Bornova, Izmir, Turkey.
authors.forename: Erol
authors.initials: E
authors.lastname: Akyilmaz
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D003543:Cysteamine / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004867:Equipment Design; D005465:Fluorocarbon Polymers / Q000737:chemistry; D006046:Gold; D006861:Hydrogen Peroxide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D055767:Immobilized Proteins / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry*; D037742:Nanotubes, Carbon; D009573:Nitrites / Q000032:analysis*; D015203:Reproducibility of Results; D013696:Temperature; D014867:Water / Q000032:analysis
chemical_list: D005465:Fluorocarbon Polymers; D055767:Immobilized Proteins; D009211:Myoglobin; D037742:Nanotubes, Carbon; D009573:Nitrites; D014867:Water; C040402:perfluorosulfonic acid; D003543:Cysteamine; D006046:Gold; D006861:Hydrogen Peroxide
keywords: Biosensor; Hydrogen peroxide; MWCNT; Myoglobin; Nafion; Nitrite