title: In vivo and in vitro changes in neurochemical parameters related to mercury concentrations from specific brain regions of polar bears (Ursus maritimus).
abstract: Mercury (Hg) has been detected in polar bear brain tissue, but its biological effects are not well known. Relationships between Hg concentrations and neurochemical enzyme activities and receptor binding were assessed in the cerebellum, frontal lobes, and occipital lobes of 24 polar bears collected from Nunavik (Northern Quebec), Canada. The concentration-response relationship was further studied with in vitro experiments using pooled brain homogenate of 12 randomly chosen bears. In environmentally exposed brain samples, there was no correlative relationship between Hg concentration and cholinesterase (ChE) activity or muscarinic acetylcholine receptor (mAChR) binding in any of the 3 brain regions. Monoamine oxidase (MAO) activity in the occipital lobe showed a negative correlative relationship with total Hg concentration. In vitro experiments, however, demonstrated that Hg (mercuric chloride and methylmercury chloride) can inhibit ChE and MAO activities and muscarinic mAChR binding. These results show that Hg can alter neurobiochemical parameters but the current environmental Hg exposure level does have an effect on the neurochemistry of polar bears from northern Canada.
authors.affiliation: Natural Resources and Environmental Studies, University of Northern British Columbia, Prince George, British Columbia, Canada.
authors.forename: Anke
authors.initials: A
authors.lastname: Krey
authors.forename: Michael
authors.initials: M
authors.lastname: Kwan
authors.forename: Hing Man
authors.initials: HM
authors.lastname: Chan
mesh_terms: D000818:Animals; D001110:Arctic Regions; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002802:Cholinesterases / Q000378:metabolism; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000032:analysis; D005260:Female; D008297:Male; D008628:Mercury / Q000032:analysis; D008767:Methylmercury Compounds / Q000032:analysis*; D008995:Monoamine Oxidase / Q000378:metabolism; D011485:Protein Binding; D011792:Quebec; D001503:Ursidae / Q000378:metabolism*
chemical_list: D004785:Environmental Pollutants; D008767:Methylmercury Compounds; D008995:Monoamine Oxidase; D002802:Cholinesterases; D008628:Mercury; C004925:methylmercuric chloride
keywords: Brain; Canadian Arctic; Mercury; Neurochemical parameter; Polar bear