title: Nyx pholeocola, a new genus and cavernicolous species of tribe Aedini (Diptera: Culicidae) from southern Thailand based on morphological and molecular data.
abstract: Nyx Harbach & Linton, gen. nov., is introduced as a new mosquito genus of tribe Aedini for a previously unknown cave-dwelling species, Nyx pholeocola Linton & Harbach, sp. nov., from southern Thailand. A diagnosis of the genus is provided that features unique anatomical characters of the adult, pupal and larval stages of the type species. The affinities of Nyx are discussed in terms of its position in the phylogeny of Aedini. Nyx is more closely related to Borichinda and Isoaedes than to other genera of tribe Aedini. Salient differences that distinguish these three genera are contrasted. The male and female genitalia, pupa and fourth-instar larva of the new species are illustrated. DNA sequence for the second nuclear internal spacer region (ITS2) and the 658-bp barcode fragment of the mitochondrial cytochrome c oxidase I (COI) gene reveal very low similarity with published sequences, supporting the unique status of the new species.
authors.forename: Ralph E
authors.initials: RE
authors.lastname: Harbach
authors.forename: Ian J
authors.initials: IJ
authors.lastname: Kitching
authors.forename: C Lorna
authors.initials: CL
authors.lastname: Culverwell
authors.forename: Theresa M
authors.initials: TM
authors.lastname: Howard
authors.forename: Yvonne-Marie
authors.initials: YM
authors.lastname: Linton
mesh_terms: D000818:Animals; D009033:Culicidae / Q000033:anatomy & histology / Q000145:classification / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D005260:Female; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007814:Larva / Q000033:anatomy & histology; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011679:Pupa / Q000033:anatomy & histology; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013785:Thailand
chemical_list: D004272:DNA, Mitochondrial; D021903:DNA, Ribosomal Spacer; D019476:Insect Proteins; D003576:Electron Transport Complex IV