title: RRHP: a tag-based approach for 5-hydroxymethylcytosine mapping at single-site resolution.
abstract: Current methods for genomic mapping of 5-hydroxymethylcytosine (5hmC) have been limited by either costly sequencing depth, high DNA input, or lack of single-base resolution. We present an approach called Reduced Representation 5-Hydroxymethylcytosine Profiling (RRHP) to map 5hmC sites at single-base resolution by exploiting the use of beta-glucosyltransferase to inhibit enzymatic digestion at the junction where adapters are ligated to a genomic library. Therefore, only library fragments presenting glucosylated 5hmC residues at the junction are sequenced. RRHP can detect sites with low 5hmC abundance, and when combined with RRBS data, 5-methylcytosine and 5-hydroxymethylcytosine can be compared at a specific site.
authors.forename: Adam
authors.initials: A
authors.lastname: Petterson
authors.forename: Tzu Hung
authors.initials: TH
authors.lastname: Chung
authors.forename: Darany
authors.initials: D
authors.lastname: Tan
authors.forename: Xueguang
authors.initials: X
authors.lastname: Sun
authors.forename: Xi-Yu
authors.initials: XY
authors.lastname: Jia
mesh_terms: D044503:5-Methylcytosine / Q000031:analogs & derivatives; D003596:Cytosine / Q000031:analogs & derivatives* / Q000502:physiology; D019175:DNA Methylation; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics; D058977:Molecular Sequence Annotation; D017422:Sequence Analysis, DNA
chemical_list: C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine