title: Metabolic imaging using nanostructure-initiator mass spectrometry (NIMS).
abstract: Nanostructure-initiator mass spectrometry (NIMS) imaging using soft laser desorption/ionization has proven to be a powerful tool in localizing the spatial distribution of intact biomolecules. NIMS specifically has been demonstrated to have high sensitivity and low background, particularly in the low mass range <1,000 Da, making this technique well suited for metabolic imaging studies. Here, we describe NIMS imaging for direct analysis of metabolite composition across a sectioned biospecimen.
authors.affiliation: Department of Bioenergy/GTL & Structural Biology, Berkeley Lab, One Cyclotron Road, Berkeley, CA, 94720, USA.
authors.forename: Katherine B
authors.initials: KB
authors.lastname: Louie
authors.forename: Trent R
authors.initials: TR
authors.lastname: Northen
mesh_terms: D000818:Animals; D016616:Cryoultramicrotomy / Q000379:methods; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D055432:Metabolomics / Q000295:instrumentation / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*