title: Soluble CD83 inhibits human monocyte differentiation into dendritic cells in vitro.
abstract: Human CD83 is type I transmembrane glycoprotein, mainly expressed on mature dendritic cells (DCs), so it was first described as a molecular marker for mature DC. However, increasing evidence has demonstrated that CD83 is also an immunomodulatory molecule either its membrane-bound CD83 (mCD83) or soluble CD83 (sCD83) released from DCs. Intriguingly, the mCD83 possesses stimulatory effects on immune response, on the contrary, the sCD83 has inhibitory effects. Whether the sCD83 has the inhibitory effects on human monocyte differentiation into DCs is unknown. To this end, we prepared the recombinant human sCD83 in HEK293T cells and treated human monocytes being differentiated into DCs in vitro with the sCD83, and evaluate sCD83 inhibitory effects on immune response by analyzing the surface marker pattern of the cells. The results showed that the sCD83, especially glycosylated sCD83 could bind the monocytes and significantly inhibited the depression of CD14 expressions (P<0.01) and reduced CD1a, CD80, CD86 and MHC II expressions (P<0.01 or P<0.05) during the differentiation, indicating that the sCD83 can inhibit monocyte differentiation into DCs, and suggesting that a negative feedback regulation may exist in monocyte differentiation into DCs based on sCD83 released from the mature DCs.
authors.affiliation: Department of Biotechnology, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China; Laboratory of Molecular Virology and Immunology, College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071001, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Lin
authors.affiliation: Department of Medicine, School of Medicine, University of California San Diego, La Jolla, CA 92093, USA.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liang
authors.affiliation: Department of Pharmacology, School of Medicine, University of California San Diego, La Jolla, CA 92093, USA.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Laboratory of Molecular Virology and Immunology, College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071001, China.
authors.forename: Jiexia
authors.initials: J
authors.lastname: Wen
authors.affiliation: Laboratory of Molecular Virology and Immunology, College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071001, China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Li
authors.affiliation: Laboratory of Molecular Virology and Immunology, College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071001, China.
authors.forename: Liyue
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Biotechnology, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.affiliation: Laboratory of Molecular Virology and Immunology, College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071001, China. Electronic address: zhongf@hebau.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhong
authors.affiliation: Department of Biotechnology, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China. Electronic address: lixiujin9824@aliyun.com.
authors.forename: Xiujin
authors.initials: X
authors.lastname: Li
mesh_terms: D051940:B7-2 Antigen / Q000276:immunology*; D002454:Cell Differentiation; D002462:Cell Membrane / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D057809:HEK293 Cells; D006801:Humans; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D012995:Solubility
chemical_list: D051940:B7-2 Antigen; C497964:CD86 protein, human
keywords: Dendritic cells; Expression; Inhibitory regulation; Monocyte differentiation; Soluble CD83