title: Collagen-poly(dialdehyde) guar gum based porous 3D scaffolds immobilized with growth factor for tissue engineering applications.
abstract: Here we report the preparation of collagen-poly(dialdehyde) guar gum based hybrid functionalized scaffolds covalently immobilized with platelet derived growth factor - BB for tissue engineering applications. Poly(dialdehyde) guar gum was synthesized from selective oxidation of guar gum using sodium periodate. The synthesized poly(dialdehyde) guar gum not only promotes crosslinking of collagen but also immobilizes the platelet derived growth factor through imine bonds. The covalent crosslinking formed in collagen improves thermal, swelling and biodegradation properties of the hybrid scaffolds. The prepared hybrid scaffolds show 3D interconnected honeycomb porous structure when viewed under a microscope. The release of immobilized platelet derived growth factor was seen up to 13th day of incubation thereby proving its sustained delivery. The developed hybrid scaffold leads to a quantum increase in NIH 3T3 fibroblast cell density and proliferation thereby demonstrating its potential for tissue engineering applications.
authors.affiliation: Advanced Materials Laboratory, Centre for Leather Apparel & Accessories Development, Central Leather Research Institute (Council of Scientific and Industrial Research), Adyar, Chennai 600020, India.
authors.forename: Murali
authors.initials: M
authors.lastname: Ragothaman
authors.affiliation: Advanced Materials Laboratory, Centre for Leather Apparel & Accessories Development, Central Leather Research Institute (Council of Scientific and Industrial Research), Adyar, Chennai 600020, India. Electronic address: thanik8@yahoo.com.
authors.forename: Thanikaivelan
authors.initials: T
authors.lastname: Palanisamy
authors.affiliation: Advanced Materials Laboratory, Centre for Leather Apparel & Accessories Development, Central Leather Research Institute (Council of Scientific and Industrial Research), Adyar, Chennai 600020, India.
authors.forename: Cheirmadurai
authors.initials: C
authors.lastname: Kalirajan
mesh_terms: D000818:Animals; D000077214:Becaplermin; D001672:Biocompatible Materials / Q000737:chemistry; D003094:Collagen / Q000737:chemistry*; D005685:Galactans / Q000737:chemistry*; D008351:Mannans / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D053149:Plant Gums / Q000737:chemistry*; D016062:Porosity; D020574:Proto-Oncogene Proteins c-sis / Q000737:chemistry / Q000145:classification; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D005685:Galactans; D008351:Mannans; D053149:Plant Gums; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D003094:Collagen; C007894:guar gum
keywords: Cellularization; Collagen; Platelet-derived growth factor; Poly(dialdehyde) guar gum; Porosity