title: Comparison of multilocus sequence typing, RAPD, and MALDI-TOF mass spectrometry for typing of β-lactam-resistant Klebsiella pneumoniae strains.
abstract: Extended spectrum of β-lactam (ESBL) resistance of Klebsiella pneumoniae has become an increasing problem in hospital infections. Typing of isolates is important to establish the intrahospital surveillance of resistant clones. In this study, the discriminatory potential of randomly amplified polymorphic DNA and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) analyses were compared with multilocus sequence typing (MLST) by using 17 β-lactam-resistant K. pneumoniae isolates of different genotypes. MLST alleles were distributed in 8 sequence types (STs). Among ESBL strains of the same ST, the presence of different β-lactamase genes was common. RAPD band patterns also revealed 8 types that corresponded to MLST-defined genotypes in 15 out of 17 cases. MALDI-TOF analysis could differentiate 5 clusters of strains. The results of this work show that RAPD may be usable as a rapid screening method for the intrahospital surveillance of K. pneumoniae, allowing a discrimination of clonally related strains. MALDI-TOF-based typing was not strongly corresponding to genotyping and warrants further investigation.
authors.affiliation: Institute of Medical Microbiology, University Hospital of Jena, D-07747 Jena, Germany.
authors.forename: Svea
authors.initials: S
authors.lastname: Sachse
authors.affiliation: Institute of Medical Microbiology, University Hospital of Jena, D-07747 Jena, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Bresan
authors.affiliation: RIPAC-LABOR, D-14476 Potsdam, Germany.
authors.forename: Marcel
authors.initials: M
authors.lastname: Erhard
authors.affiliation: Institute of Medical Microbiology, University Hospital of Jena, D-07747 Jena, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Edel
authors.affiliation: Institute of Medical Microbiology, University Hospital of Jena, D-07747 Jena, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Pfister
authors.affiliation: Institute of Medical Microbiology, University Hospital of Jena, D-07747 Jena, Germany.
authors.forename: Angela
authors.initials: A
authors.lastname: Saupe
authors.affiliation: Institute of Medical Microbiology, University Hospital of Jena, D-07747 Jena, Germany. Electronic address: juergen.roedel@med.uni-jena.de.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Rödel
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods; D024881:Drug Resistance, Bacterial; D006801:Humans; D007362:Intensive Care Units; D007710:Klebsiella Infections / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D058885:Multilocus Sequence Typing / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D001618:beta-Lactamases / Q000235:genetics; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D047090:beta-Lactams; D001618:beta-Lactamases
keywords: ESBL; Klebsiella; MALDI-TOF; MLST; RAPD; Typing