title: Characteristics of spermatogonial stem cells derived from neonatal porcine testis.
abstract: The aim of this study was to isolate and characterise porcine spermatogonial stem cells (PSSCs). The putative porcine germline stem cells from testis were isolated successfully by an improving way of enrichment with lymphocyte separation medium (LSM). Results from RT-PCR analyses showed that PSSCs were positive for OCT4, SOX2, NANOG, PGP9.5, c-MYC, KEL4 and PRDM-14 which are multipotent stem cell markers. At the protein level, the results of immunofluorescence analyses showed that PSSCs were positive for OCT4, PGP9.5, SOX2 and CD29. We successfully differentiated these PSSCs into adipocytes and muscle cells and then defined their characteristics, including morphology, surface stem cell markers, and mechanical properties. But the experiment of teratoma formation was negative. The results indicated the PSSCs could be multipotent. Atomic force microscopy was used to characterise the morphological and mechanical properties of undifferentiated PSSCs, as well as the differentiated adipocytes and muscle cells, which could be potentially useful for distinguishing PSSCs from differentiated cells.
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China.|Key Laboratory of Cellular Physiology, Ministry of Education, Department of Cell Biology and Genetics, Shanxi Medical University, Taiyuan, Shanxi, China.
authors.forename: R
authors.initials: R
authors.lastname: Shi
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Life Science and Technology College, Jinan University, Guangzhou, China.
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China.
authors.forename: H
authors.initials: H
authors.lastname: Wei
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China.|South-China Primate Research and Development Center, Guangdong Entomological Institute, Guangzhou, China.
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China.
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Animal Science/Center for Regenerative Biology, University of Connecticut, Storrs, CT, USA.
authors.forename: X C
authors.initials: XC
authors.lastname: Tian
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China.
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China.
authors.forename: L
authors.initials: L
authors.lastname: Qin
authors.affiliation: Life Science and Technology College, Jinan University, Guangzhou, China.
authors.forename: J
authors.initials: J
authors.lastname: Cai
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China.
authors.forename: S
authors.initials: S
authors.lastname: Zhang
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D008297:Male; D051379:Mice; D008819:Mice, Nude; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D009132:Muscles / Q000166:cytology; D013093:Spermatogonia / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D013552:Swine; D013724:Teratoma / Q000473:pathology; D013737:Testis / Q000166:cytology*
keywords: Adipocyte; characteristics; mechanical properties; muscular cells; porcine spermatogonial stem cells