title: Liquefaction of sugarcane bagasse for enzyme production.
abstract: The objective of this paper is to report liquefaction of pretreated and sterilized sugarcane bagasse for enhancing endoglucanase production through submerged fermentation by Aspergillus niger. After initial solid state fermentation of steam pretreated bagasse solids by A. niger, fed-batch addition of the substrate to cellulase in buffer over a 12h period, followed by 36h reaction, resulted in a liquid slurry with a viscosity of 0.30±0.07Pas at 30% (w/v) solids. Addition of A. niger for submerged fermentation of sterile liquefied bagasse at 23% w/v solids resulted in an enzyme titer of 2.5IUmL(-1) or about 15× higher productivity than solid-state fermentation of non-liquefied bagasse (final activity of 0.17IUmL(-1)). Bagasse not treated by initial solid-state fermentation but liquefied with enzyme gave 2IUmL(-1). These results show the utility of liquefied bagasse as a culture medium for enzyme production in submerged fermentations.
authors.affiliation: Laboratory of Renewable Resources Engineering and Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA; Graduate Program of Chemical Engineering, Federal University of São Carlos, C.P. 676, 13565-905 São Carlos, SP, Brazil; Embrapa Instrumentation, Rua XV de Novembro 1452, 13560-970 São Carlos, SP, Brazil.
authors.forename: F M
authors.initials: FM
authors.lastname: Cunha
authors.affiliation: Laboratory of Renewable Resources Engineering and Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: T
authors.initials: T
authors.lastname: Kreke
authors.affiliation: Graduate Program of Chemical Engineering, Federal University of São Carlos, C.P. 676, 13565-905 São Carlos, SP, Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: Badino
authors.affiliation: Graduate Program of Chemical Engineering, Federal University of São Carlos, C.P. 676, 13565-905 São Carlos, SP, Brazil; Embrapa Instrumentation, Rua XV de Novembro 1452, 13560-970 São Carlos, SP, Brazil.
authors.forename: C S
authors.initials: CS
authors.lastname: Farinas
authors.affiliation: Laboratory of Renewable Resources Engineering and Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: E
authors.initials: E
authors.lastname: Ximenes
authors.affiliation: Laboratory of Renewable Resources Engineering and Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA. Electronic address: ladisch@purdue.edu.
authors.forename: M R
authors.initials: MR
authors.lastname: Ladisch
mesh_terms: D001234:Aspergillus niger / Q000378:metabolism*; D002480:Cellulase / Q000096:biosynthesis*; D005285:Fermentation; D031786:Saccharum
chemical_list: D002480:Cellulase
keywords: Aspergillus niger; Cellulase production; Fermentation; Liquefaction; Sugarcane bagasse