title: Preliminary characterization, antioxidant properties and production of chrysolaminarin from marine diatom Odontella aurita.
abstract: A new chrysolaminarin, named CL2, with a molecular mass of 7.75 kDa, was purified from the marine diatom, Odontella aurita, using DEAE-52 cellulose anion-exchange chromatography and Sephadex G-200 gel-filtration chromatography. The monosaccharide and structural analysis revealed that CL2 was a glucan mainly composed of glucose, which was linked by the β-d-(1→3) (main chain) and β-d-(1→6) (side chain) glycosidic bond, demonstrated by infrared spectroscopy (IR) and nuclear magnetic resonance (NMR). The antioxidant activity tests revealed that the CL2 presented stronger hydroxyl radical scavenging activity with increasing concentrations, but less was effective on reducing power analysis and scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. The influences of nitrogen concentration and light intensity on chrysolaminarin production of O. aurita were further investigated in a glass column photobioreactor, and a record high chrysolaminarin productivity of 306 mg L-1 day-1 was achieved. In conclusion, the chrysolaminarin CL2 from O. aurita may be explored as a natural antioxidant agent for application in aquaculture, food and pharmaceutical areas.
authors.affiliation: Institute of Hydrobiology, Jinan University, Guangzhou 510632, China. xiasongsummer212@163.com.
authors.forename: Song
authors.initials: S
authors.lastname: Xia
authors.affiliation: Institute of Hydrobiology, Jinan University, Guangzhou 510632, China. gaobaoyan1211@126.com.
authors.forename: Baoyan
authors.initials: B
authors.lastname: Gao
authors.affiliation: Institute of Hydrobiology, Jinan University, Guangzhou 510632, China. tiger@jnu.edu.cn.
authors.forename: Aifen
authors.initials: A
authors.lastname: Li
authors.affiliation: Institute of Energy Research, Jiangxi Academy of Sciences, Nanchang 330096, China. xjh6110@vip.sina.com.
authors.forename: Jihai
authors.initials: J
authors.lastname: Xiong
authors.affiliation: Institute of Energy Research, Jiangxi Academy of Sciences, Nanchang 330096, China. aoziqiang628@163.com.
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Ao
authors.affiliation: Institute of Hydrobiology, Jinan University, Guangzhou 510632, China. tzhangcw@jnu.edu.cn.
authors.forename: Chengwu
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018533:Biomass; D019149:Bioreactors; D001713:Biphenyl Compounds; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D017377:Diatoms / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005936:Glucans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017665:Hydroxyl Radical; D008970:Molecular Weight; D010851:Picrates; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005936:Glucans; D010851:Picrates; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl