title: Propolis enhances the effectiveness of praziquantel in experimental schistosomiasis: biochemical and histopathological study.
abstract: Despite the wide current use of praziquantel (PZQ) in treatment of schistosomiasis, low cure rates have been recorded in many studies. The aim of this study was directed to evaluate the curative effect of propolis (Pps) alone or in combination with PZQ on biochemical, immunological, parasitological, and histological changes associated with experimental schistosomiasis in mice. Schistosoma mansoni-infected mice were divided into two experimental sets, each with four subgroups: (i) untreated, (ii) treated with Pps/day p.o for 4 weeks, (iii) treated with PZQ p.o 2 × 500 mg/kg bd wt, and (iv) treated with Pps + PZQ as in group ii and iii; all treatments started on the 8th week postinfection, in addition to uninfected group as control for the previous groups. Treatment of infected mice with Pps, although failed to eradicate the worm, significantly reduced the hepatic granuloma number, their lymphocytic infiltration and aggregation, hepatic and splenic myeloperoxidase (MPO) activity and plasma, and liver and thymus nitric oxide (NOx) levels together with normalization of plasma proteins and alleviation of oxidative stress in the examined tissues as evidenced by reduction of malondialdehyde (MDA) and normalization of glutathione (GSH). Promising results were obtained when Pps was given in combination with PZQ, where the anti-schistosomal activity of PZQ was markedly potentiated with complete alleviation and amelioration of the histological and biochemical alteration associated with schistosomiasis. This study highlights the potential usefulness of Pps as an adjunct to PZQ in schistosomiasis.
authors.affiliation: Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
authors.forename: Tamer Y
authors.initials: TY
authors.lastname: Mahmoud
authors.forename: Sherine M
authors.initials: SM
authors.lastname: Rizk
authors.forename: Amany S
authors.initials: AS
authors.lastname: Maghraby
authors.forename: Amira A
authors.initials: AA
authors.lastname: Shaheen
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000627:therapeutic use*; D001798:Blood Proteins / Q000032:analysis; D004359:Drug Therapy, Combination; D005978:Glutathione / Q000378:metabolism; D006099:Granuloma / Q000473:pathology; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008099:Liver / Q000469:parasitology / Q000473:pathology; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism; D011168:Portal System / Q000469:parasitology; D011223:Praziquantel / Q000494:pharmacology / Q000627:therapeutic use*; D011429:Propolis / Q000494:pharmacology / Q000627:therapeutic use*; D011897:Random Allocation; D012550:Schistosoma mansoni / Q000187:drug effects / Q000254:growth & development; D012555:Schistosomiasis mansoni / Q000188:drug therapy*
chemical_list: D000890:Anti-Infective Agents; D001798:Blood Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009569:Nitric Oxide; D008315:Malondialdehyde; D011223:Praziquantel; D011429:Propolis; D009195:Peroxidase; D005978:Glutathione