title: [Expression of IL-35, Epstein-Barr virus-induced gene 3 mRNA and IL-12A mRNA in peripheral blood of patients with allergic rhinitis].
abstract: OBJECTIVE
  To explore the expression of IL-35, Epstein-Barr virus-induced gene 3 (EBI3) mRNA and IL-12A mRNA in peripheral blood of patients with allergic rhinitis and its significance.
  
  
  METHODS
  Peripheral blood were collected from patients with allergic rhinitis (46 cases) and healthy human controls(30 cases). The level of IL-35 in serum was measured by enzyme-linked immunosorbent assay. The subunit EB13 and IL-12A of IL-35 mRNA expressions in peripheral blood mononuclear cell(PBMC) were detected by SYBR Green real-time quantitative PCR.
  
  
  RESULTS
  IL-35 level in AR group (251.22 +/- 46.27) ng/L was significantly lower compared with that in the normal control group (382.17 +/- 25.41) ng/L, (P < 0.01). The mRNA expression level of EBI3 in the patients with allergic rhinitis was significantly lower than that in the normal control group (P < 0.01), AR group EB13 mRNA level was about half of that in the normal control group. But the mRNA expression level of IL-12A in the patients with allergic rhinitis has not significant difference with that in the normal control group (P > 0. 05).
  
  
  CONCLUSIONS
  The decrease of IL-35 and EBI3 mRNA in AR,indicated that IL-35 and EBl3 mRNA may play an important role in allergic rhinitis.
authors.forename: Jun
authors.initials: J
authors.lastname: Wan
authors.forename: Ying
authors.initials: Y
authors.lastname: Luo
authors.forename: Chunping
authors.initials: C
authors.lastname: Yang
authors.forename: Jiali
authors.initials: J
authors.lastname: Liu
authors.forename: Yunyan
authors.initials: Y
authors.lastname: Du
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002648:Child; D005260:Female; D006801:Humans; D053765:Interleukin-12 Subunit p35 / Q000097:blood*; D007378:Interleukins / Q000097:blood*; D008297:Male; D008875:Middle Aged; D015778:Minor Histocompatibility Antigens; D065631:Rhinitis, Allergic / Q000097:blood*; D055815:Young Adult
chemical_list: C097732:EBI3 protein, human; C508858:IL12A protein, human; D053765:Interleukin-12 Subunit p35; D007378:Interleukins; D015778:Minor Histocompatibility Antigens; C532417:interleukin-35, human