title: Loss of Ypk1, the yeast homolog to the human serum- and glucocorticoid-induced protein kinase, accelerates phospholipase B1-mediated phosphatidylcholine deacylation.
abstract: Ypk1, the yeast homolog of the human serum- and glucocorticoid-induced kinase (Sgk1), affects diverse cellular activities, including sphingolipid homeostasis. We now report that Ypk1 also impacts the turnover of the major phospholipid, phosphatidylcholine (PC). Pulse-chase radiolabeling reveals that a ypk1Δ mutant exhibits increased PC deacylation and glycerophosphocholine production compared with wild type yeast. Deletion of PLB1, a gene encoding a B-type phospholipase that hydrolyzes PC, in a ypk1Δ mutant curtails the increased PC deacylation. In contrast to previous data, we find that Plb1 resides in the ER and in the medium. Consistent with a link between Ypk1 and Plb1, the levels of both Plb1 protein and PLB1 message are elevated in a ypk1Δ strain compared with wild type yeast. Furthermore, deletion of PLB1 in a ypk1Δ mutant exacerbates phenotypes associated with loss of YPK1, including slowed growth and sensitivity to cell wall perturbation, suggesting that increased Plb1 activity buffers against the loss of Ypk1. Because Plb1 lacks a consensus phosphorylation site for Ypk1, we probed other processes under the control of Ypk1 that might be linked to PC turnover. Inhibition of sphingolipid biosynthesis by the drug myriocin or through utilization of a lcb1-100 mutant results in increased PLB1 expression. Furthermore, we discovered that the increase in PLB1 expression observed upon inhibition of sphingolipid synthesis or loss of Ypk1 is under the control of the Crz1 transcription factor. Taken together, these results suggest a functional interaction between Ypk1 and Plb1 in which altered sphingolipid metabolism up-regulates PLB1 expression via Crz1.
authors.affiliation: From the Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania 15282 and.
authors.forename: Beth A
authors.initials: BA
authors.lastname: Surlow
authors.affiliation: From the Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania 15282 and.
authors.forename: Benjamin M
authors.initials: BM
authors.lastname: Cooley
authors.affiliation: the Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260.
authors.forename: Patrick G
authors.initials: PG
authors.lastname: Needham
authors.affiliation: the Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260.
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Brodsky
authors.affiliation: From the Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania 15282 and pattonvogt@duq.edu.
authors.forename: Jana
authors.initials: J
authors.lastname: Patton-Vogt
mesh_terms: D000107:Acetylation; D000483:Alleles; D002794:Choline / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005997:Glycerylphosphorylcholine / Q000378:metabolism; D038362:Glycogen Synthase Kinase 3 / Q000235:genetics* / Q000378:metabolism*; D006706:Homeostasis; D006868:Hydrolysis; D008055:Lipids / Q000737:chemistry; D008245:Lysophospholipase / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D010713:Phosphatidylcholines / Q000378:metabolism*; D010766:Phosphorylation; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D013107:Sphingolipids / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: C109868:CRZ1 protein, S cerevisiae; D004268:DNA-Binding Proteins; D005229:Fatty Acids, Monounsaturated; D008055:Lipids; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D029701:Saccharomyces cerevisiae Proteins; D013107:Sphingolipids; D014157:Transcription Factors; D005997:Glycerylphosphorylcholine; D038362:Glycogen Synthase Kinase 3; C068124:MCK1 protein, S cerevisiae; D008245:Lysophospholipase; C490270:PLB1 protein, S cerevisiae; D002794:Choline; C001996:thermozymocidin
keywords: Glycerophosphocholine; PLB1; Phosphatidylcholine; Phospholipase; Phospholipid Turnover; Protein Kinase; SGK1; Sphingolipid; Yeast