title: Zinc glycine chelate absorption characteristics in Sprague Dawley rat.
abstract: This study was conducted to investigate absorption characteristics of zinc glycine chelate (Zn-Gly) by evaluating tissues zinc status and the expression of zinc transporters in rats. A total of 24 male rats were randomly allocated to three treatments and administered either saline or 35 mg Zn/kg body weight from zinc sulphate (ZnSO4 ) or Zn-Gly by feeding tube separately. Four rats per group were slaughtered and tissues were collected at 2 and 6 h after gavage respectively. Our data showed that Zn-Gly did more effectively in increasing (p < 0.05) serum zinc levels, and the activities of serum and liver alkaline phosphatase (ALP) and liver Cu/Zn superoxide dismutase (Cu/Zn SOD) at 2 and 6 h. By 2 h after the zinc load, the mRNA and protein abundance of intestinal metallothionein1 (MT1) and zinc transporter SLC30A1 (ZnT1) were higher (p < 0.05), and zinc transporter SLC39A4 (Zip4) lower (p < 0.05) in ZnSO4 compared to other groups. Zinc transporter SLC39A5 (Zip5) mRNA expression was not zinc responsive, but Zip5 protein abundance was remarkably (p < 0.05) increased in ZnSO4 2 h later. Overall, our results indicated that in short-term periods, Zn-Gly was more effective in improving body zinc status than ZnSO4 , and ZnSO4 did more efficiently on the regulation of zinc transporters in small intestine.
authors.affiliation: The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Sciences, Zhejiang University, Hangzhou, China.
authors.forename: M
authors.initials: M
authors.lastname: Yue
authors.forename: S L
authors.initials: SL
authors.lastname: Fang
authors.forename: Z
authors.initials: Z
authors.lastname: Zhuo
authors.forename: D D
authors.initials: DD
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Feng
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D005998:Glycine / Q000737:chemistry*; D008099:Liver / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015032:Zinc / Q000097:blood / Q000737:chemistry* / Q000493:pharmacokinetics* / Q000494:pharmacology
chemical_list: D002352:Carrier Proteins; D012333:RNA, Messenger; D015032:Zinc; D005998:Glycine
keywords: laboratory animals; zinc glycine chelate; zinc metabolism; zinc sulphate; zinc transporters