title: Correlation of the interleukin-29 levels in crevicular fluid and plasma with the genetic polymorphism in chronic and aggressive periodontitis patients.
abstract: OBJECTIVE
  To assess the effect of single nucleotide polymorphism (SNP) on the Interleukin (IL-29) quantity in gingival crevicular fluid (GCF) and plasma of chronic and aggressive periodontitis patients.
  
  
  METHODS
  Patients with periodontal health (n=30), chronic generalized periodontitis (n=30) and generalized aggressive periodontitis (n=30) were subjected to IL-29 quantity estimation in GCF and plasma using enzyme linked immunosorbent assay and was correlated with IL-29 SNP (rs30461) using polymerase chain reaction.
  
  
  RESULTS
  IL-29 concentration in GCF and plasma was highest in aggressive periodontitis patients (114.17 ± 95.07pg/ml and 149.69 ± 109.90pg/ml respectively). The least concentration was found in subjects with healthy periodontium (47.50 ± 37.75pg/ml and 54.52 ± 37.53pg/ml) and in chronic periodontitis it was found to be 65.01 ± 41.26pg/ml and 81.17 ± 46.23pg/ml. The difference in the quantity of IL-29 in GCF and plasma among different groups was statistically significant (p<0.001 and p<0.001 respectively). rs30461 polymorphism of IL-29 analysis revealed that difference in the prevalence of A/A, A/G and G/G genotype among three groups was not statistically significant (p=0.097).
  
  
  CONCLUSIONS
  Increased quantity of IL-29 in GCF and plasma of subjects with periodontitis suggests a role in pathogenesis of periodontitis and the SNP (rs30461) is not related to susceptibility to periodontitis in this population of Indian individuals.
authors.affiliation: Ph.D Research Scholar, Department of Periodontology, Government Dental College and Research Institute, Bangalore, India; Reader, Department of Periodontology, Rajarajeswari Dental College and Hospital, Bangalore, India. Electronic address: drshivaprasad2008@yahoo.co.in.
authors.forename: B M
authors.initials: BM
authors.lastname: Shivaprasad
authors.affiliation: Professor and Head, Department of Periodontology, Government Dental College and Research Institute, Bangalore, India. Electronic address: periodonticsgdcri@gmail.com.
authors.forename: A R
authors.initials: AR
authors.lastname: Pradeep
mesh_terms: D000328:Adult; D010520:Aggressive Periodontitis / Q000235:genetics* / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D055113:Chronic Periodontitis / Q000235:genetics* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D007194:India; D007372:Interferons; D007378:Interleukins / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D000095022:Interferon Lambda
chemical_list: D015415:Biomarkers; D007372:Interferons; D007378:Interleukins; C000624353:interferon-lambda, human; D000095022:Interferon Lambda
keywords: Aggressive periodontitis; Chronic periodontitis; Herpes viruses; Interferon-lambda; Interleukin-29; Single nucleotide polymorphism