title: [Effects of amino acid on growth and secondary metabolites contents of adventitious roots of Tripterygium wilfordii].
abstract: The adventitious root of Tripterygium wilfordii was used as experiment material to study effects of various concentration of aspartic acid, isoleucine, cysteine and arginine in MS medium on the growth and triptolide, wilforgine, wilforine contents of the adventitious roots. The results showed that compared with the control, supplemented with 0.25 mmol x L(-1) aspartic acid at 3rd week, the growth of the adventitious roots only accounted for 80%, but the content of triptolide of the adventitious roots and the medium was 1.36, 1.30 times, the content of wilforgine was 1.16, 1.37 times, the content of wilforine was 1.22, 1.63 times, respectively. At 3rd week 0.05 mmol x L(-1) isoleucine, the growth of adventitious roots was 97.3%, wilforgine of adventitious roots and medium 1.02, 1.27 times, wilforine 1.36 times and 1.15 times. At 1st week 0.25 mmol x L(-1) cysteine, the growth of the adventitious roots comprised 77.5% of the control, while content of triptolide of adventitious roots reached 1.87 times. At 2nd week 1.00 mmol x L(-1) cysteine, the growth of adventitious roots was 44.6% of the control, the content of wilforine in medium was 2.97 times. At 3rd week 0.50 mmol x L(-1) arginine, the growth of adventitious roots was 124.2%, the content of wilforgine and wilforine was 1.3, 1.4 times, respectively.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Wen
authors.forename: Jia-Min
authors.initials: JM
authors.lastname: Lei
authors.forename: Qun
authors.initials: Q
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Cui
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000378:metabolism*; D004224:Diterpenes / Q000032:analysis; D004852:Epoxy Compounds / Q000032:analysis; D007783:Lactones / Q000032:analysis / Q000378:metabolism; D010616:Phenanthrenes / Q000032:analysis; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D011725:Pyridines / Q000032:analysis / Q000378:metabolism; D064210:Secondary Metabolism; D029757:Tripterygium / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D004224:Diterpenes; D004852:Epoxy Compounds; D007783:Lactones; D010616:Phenanthrenes; D011725:Pyridines; C479287:wilforgine; C001899:triptolide