title: Proteomic analysis of nasal epithelial cells from cystic fibrosis patients.
abstract: The pathophysiology of cystic fibrosis (CF) lung disease remains incompletely understood. New explanations for the pathogenesis of CF lung disease may be discovered by studying the patterns of protein expression in cultured human nasal epithelial cells (HNEC). To that aim, we compared the level of protein expressions in primary cultures of HNEC from nasal polyps secondary to CF (CFNP, n = 4), primary nasal polyps (NP, n = 8) and control mucosa (CTRL, n = 4) using isobaric tag for relative and absolute quantification (iTRAQ) labeling coupled with liquid chromatography (LC)-MS-MS. The analysis of the data revealed 42 deregulated protein expressions in CFNP compared to NP and CTRL, suggesting that these alterations are related to CF. Overall, AmiGo analysis highlighted six major pathways important for cell functions that seem to be impaired: metabolism, G protein process, inflammation and oxidative stress response, protein folding, proteolysis and structural proteins. Among them, glucose and fatty acid metabolic pathways could be impaired in CF with nine deregulated proteins. Our proteomic study provides a reproducible set of differentially expressed proteins in airway epithelial cells from CF patients and reveals many novel deregulated proteins that could lead to further studies aiming to clarify the involvement of such proteins in CF pathophysiology.
authors.affiliation: Service de Génétique et Embryologie Médicales, Unité Mixte de Recherche_Scientifique 933, Institut National de la Santé et de la Recherche Médicale, Université Pierre et Marie Curie - Paris 6, and Assistance Publique - Hôpitaux de Paris, Hôpital Armand Trousseau, Paris, France; Unité_1151, Institut National de la Santé et de la Recherche Médicale, Université Paris Descartes, Paris, France.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Jeanson
authors.affiliation: Unité_1151, Institut National de la Santé et de la Recherche Médicale, Université Paris Descartes, Paris, France; Plateau Proteome Necker, Structure Fédérative de Recherche de Necker, Université Paris Descartes, Paris, France.
authors.forename: Ida Chiara
authors.initials: IC
authors.lastname: Guerrera
authors.affiliation: Unité Mixte de Recherche_Scientifique 855, Institut National de la Santé et de la Recherche Médicale, Université Paris 12, Faculté de Médecine, Créteil, France; Service d'Otorhinolaryngologie et de chirurgie cervico-faciale, Assistance Publique - Hôpitaux de Paris, Hôpital inter-communal et Groupe Hospitalier Henri Mondor-Albert Chenevier, Créteil, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Papon
authors.affiliation: Unité_1151, Institut National de la Santé et de la Recherche Médicale, Université Paris Descartes, Paris, France; Plateau Proteome Necker, Structure Fédérative de Recherche de Necker, Université Paris Descartes, Paris, France.
authors.forename: Cerina
authors.initials: C
authors.lastname: Chhuon
authors.affiliation: Unité Mixte de Recherche_Scientifique 855, Institut National de la Santé et de la Recherche Médicale, Université Paris 12, Faculté de Médecine, Créteil, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: Zadigue
authors.affiliation: Service d'Otorhinolaryngologie et de chirurgie cervico-faciale, Assistance Publique - Hôpitaux de Paris, Hôpital inter-communal et Groupe Hospitalier Henri Mondor-Albert Chenevier, Créteil, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Prulière-Escabasse
authors.affiliation: Service de Génétique et Embryologie Médicales, Unité Mixte de Recherche_Scientifique 933, Institut National de la Santé et de la Recherche Médicale, Université Pierre et Marie Curie - Paris 6, and Assistance Publique - Hôpitaux de Paris, Hôpital Armand Trousseau, Paris, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Amselem
authors.affiliation: Service de Génétique et Embryologie Médicales, Unité Mixte de Recherche_Scientifique 933, Institut National de la Santé et de la Recherche Médicale, Université Pierre et Marie Curie - Paris 6, and Assistance Publique - Hôpitaux de Paris, Hôpital Armand Trousseau, Paris, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Escudier
authors.affiliation: Unité Mixte de Recherche_Scientifique 855, Institut National de la Santé et de la Recherche Médicale, Université Paris 12, Faculté de Médecine, Créteil, France; Service d'Otorhinolaryngologie et de chirurgie cervico-faciale, Assistance Publique - Hôpitaux de Paris, Hôpital inter-communal et Groupe Hospitalier Henri Mondor-Albert Chenevier, Créteil, France.
authors.forename: André
authors.initials: A
authors.lastname: Coste
authors.affiliation: Unité_1151, Institut National de la Santé et de la Recherche Médicale, Université Paris Descartes, Paris, France; Plateau Proteome Necker, Structure Fédérative de Recherche de Necker, Université Paris Descartes, Paris, France.
authors.forename: Aleksander
authors.initials: A
authors.lastname: Edelman
mesh_terms: D000293:Adolescent; D000328:Adult; D002853:Chromatography, Liquid; D003550:Cystic Fibrosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics* / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism; D006801:Humans; D008297:Male; D053858:Metabolic Networks and Pathways; D009154:Mutation; D009298:Nasal Polyps / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress; D061251:Primary Cell Culture; D017510:Protein Folding; D059748:Proteolysis; D020543:Proteome / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D020545:Respiratory Mucosa / Q000378:metabolism* / Q000473:pathology; D053719:Tandem Mass Spectrometry
chemical_list: C505032:CFTR protein, human; D005227:Fatty Acids; D020543:Proteome; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D005947:Glucose