title: Carbon nanodots prepared from o-phenylenediamine for sensing of Cu(2+) ions in cells.
abstract: A simple hydrothermal method was applied to prepare carbon nanodots (C dots) from o-phenylenediamine (OPD). The C dots exhibit photoluminescence at 567 nm when excited at 420 nm. In the presence of Cu(2+) ions, the colour of C dots changes from yellow to orange, with an increased PL intensity as a result of the formation of Cu(OPD)2 complexes on the surfaces of C dots. The D-band to G-band ratios of C dots in the absence and presence of 80 nM Cu(2+) ions are 1.31 and 4.75, respectively. The C dots allow the detection of Cu(2+) ions with linearity over a concentration range of 2-80 nM, with a limit of detection of 1.8 nM at a signal-to-noise ratio of 3. The cell viability values of A549, MCF-10A, and MDA-MB-231 cells treated with 3 μg mL(-1) of C dots are all greater than 99%, showing their great biocompatibility. Having great water dispersibility, photostability, chemical stability (against NaCl up to 0.5 M), great selectivity, and biocompatibility, the C dots have been employed for the localization of Cu(2+) ions in the cancer cells (A549 cells) treated with 10 μM Cu(2+) ions.
authors.affiliation: Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei, 10617, Taiwan. changht@ntu.edu.tw.
authors.forename: Mani
authors.initials: M
authors.lastname: Vedamalai
authors.forename: Arun Prakash
authors.initials: AP
authors.lastname: Periasamy
authors.forename: Chia-Wei
authors.initials: CW
authors.lastname: Wang
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Tseng
authors.forename: Lin-Chen
authors.initials: LC
authors.lastname: Ho
authors.forename: Chung-Chien
authors.initials: CC
authors.lastname: Shih
authors.forename: Huan-Tsung
authors.initials: HT
authors.lastname: Chang
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival; D003300:Copper / Q000737:chemistry*; D004583:Electrons; D006801:Humans; D007477:Ions; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010655:Phenylenediamines / Q000737:chemistry*; D059629:Signal-To-Noise Ratio; D012965:Sodium Chloride; D014867:Water
chemical_list: D001672:Biocompatible Materials; D007477:Ions; D037742:Nanotubes, Carbon; D010655:Phenylenediamines; D014867:Water; D012965:Sodium Chloride; D002244:Carbon; D003300:Copper; C034193:1,2-diaminobenzene