title: Determination of exogenous epigallocatechin gallate peracetate in mouse plasma using liquid chromatography with quadrupole time-of-flight mass spectrometry.
abstract: A robust method for the quantitation of epigallocatechin gallate peracetate in plasma for pharmacokinetic studies is lacking. We have developed a validated method to quantify this compound using liquid chromatography with quadrupole time-of-flight mass spectrometry with isopropanol and tert-butyl methyl ether (3:10) extraction and thin-layer chromatography purification. The epigallocatechin gallate peracetate-1-(13) C8 isotope was used as an internal standard. The linear range (r(2) > 0.9950) was from 0.05 to 100.00 μg/mL. The lower limit of quantification of the method was 0.05 μg/mL. Reproducibility, coefficient of variation, was between 0.7 and 12.6% (n = 6), accuracy between 83.7 and 104.6% (n = 5), and recovery ranged from 82.4 to 109.0% (n = 4). Ion suppression was approximately 40%. No mass spectral peaks were found to interfere between the standard and internal standard or the blank plasma extracts. Epigallocatechin gallate peracetate in plasma was stably stored at -80°C over three months even after three freeze-thaw cycles. Extracts were stable in the sampler at 4°C for over 48 h. Plasma levels were maintained at 1.36 μg/mL for 360 min after intraorbital intravenous injection at 50 mg/kg in mice. This method can be used to reliably measure epigallocatechin gallate peracetate in plasma for pharmacokinetic studies.
authors.affiliation: Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong Eye Hospital, Kowloon, Hong Kong; Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong.
authors.forename: Kai On
authors.initials: KO
authors.lastname: Chu
authors.forename: Gene Chi Wai
authors.initials: GC
authors.lastname: Man
authors.forename: Kwok Ping
authors.initials: KP
authors.lastname: Chan
authors.forename: Ching Yan
authors.initials: CY
authors.lastname: Chu
authors.forename: Tak Hang
authors.initials: TH
authors.lastname: Chan
authors.forename: Chi Pui
authors.initials: CP
authors.lastname: Pang
authors.forename: Chi Chiu
authors.initials: CC
authors.lastname: Wang
mesh_terms: D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D005260:Female; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D008813:Mice, Inbred ICR; D010463:Peracetic Acid / Q000032:analysis*; D010949:Plasma / Q000737:chemistry / Q000166:cytology*
chemical_list: D002392:Catechin; C045651:epigallocatechin gallate; D010463:Peracetic Acid
keywords: Epigallocatechin gallate peracetate; Liquid chromatography; Mass spectrometry; Plasma profile; Validation