title: Binding sites of miR-1273 family on the mRNA of target genes.
abstract: This study examined binding sites of 2,578 miRNAs in the mRNAs of 12,175 human genes using the MirTarget program. It found that the miRNAs of miR-1273 family have between 33 and 1,074 mRNA target genes, with a free hybridization energy of 90% or more of its maximum value. The miR-1273 family consists of miR-1273a, miR-1273c, miR-1273d, miR-1273e, miR-1273f, miR-1273g-3p, miR-1273g-5p, miR-1273h-3p, and miR-1273h-5p. Unique miRNAs (miR-1273e, miR-1273f, and miR-1273g-3p) have more than 400 target genes. We established 99 mRNA nucleotide sequences that contain arranged binding sites for the miR-1273 family. High conservation of each miRNA binding site in the mRNA of the target genes was found. The arranged binding sites of the miR-1273 family are located in the 5'UTR, CDS, or 3'UTR of many mRNAs. Five repeating sites containing some of the miR-1273 family's binding sites were found in the 3'UTR of several target genes. The oligonucleotide sequences of miR-1273 binding sites located in CDSs code for homologous amino acid sequences in the proteins of target genes. The biological role of unique miRNAs was also discussed.
authors.affiliation: National Nanotechnology Laboratory, Al-Farabi Kazakh National University, Al-Farabi 71, Almaty 050038, Kazakhstan.
authors.forename: Anatoly
authors.initials: A
authors.lastname: Ivashchenko
authors.affiliation: National Nanotechnology Laboratory, Al-Farabi Kazakh National University, Al-Farabi 71, Almaty 050038, Kazakhstan.
authors.forename: Olga
authors.initials: O
authors.lastname: Berillo
authors.affiliation: National Nanotechnology Laboratory, Al-Farabi Kazakh National University, Al-Farabi 71, Almaty 050038, Kazakhstan.
authors.forename: Anna
authors.initials: A
authors.lastname: Pyrkova
authors.affiliation: National Nanotechnology Laboratory, Al-Farabi Kazakh National University, Al-Farabi 71, Almaty 050038, Kazakhstan.
authors.forename: Raigul
authors.initials: R
authors.lastname: Niyazova
mesh_terms: D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics*; D017124:Conserved Sequence; D030541:Databases, Genetic; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger