title: AlCl3 induces lymphocyte apoptosis in rats through the mitochondria-caspase dependent pathway.
abstract: To investigate apoptosis mechanisms in lymphocytes induced by aluminum trichloride (AlCl3) through the mitochondria-caspase dependent pathway, the spleen lymphocytes of rats were cultured with RPMI-1640 medium and exposed to AlCl3·6H2O in the final concentrations of 0 (control group, CG), 0.3 (low-dose group, LG), 0.6 (mid-dose group, MG), and 1.2 (high-dose group, HG) mmol·L(-1) for 24 h, respectively. Mitochondrial transmembrane potential (ΔΨm), cytochrome C (Cyt C) protein expression in cytoplasm, Caspase-3 and Caspase-9 activity, Bcl-2, Bax, Caspase-3 and Caspase-9 mRNA expressions, DNA ladder and lymphocytes apoptosis index were detected. The results showed that Cyt C protein expression in cytoplasm, Caspase-3 and Caspase-9 activity, Bcl-2, Bax, Caspase-3 and Caspase-9 mRNA expressions, the ratio of Bcl-2 and Bax mRNA expression, lymphocytes apoptosis index increased, while ΔΨm decreased in the AlCl3-treated groups compared with those in CG. The results indicate that AlCl3 induces lymphocyte apoptosis in rats through the mitochondria-caspase dependent pathway.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Ren
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Xiu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.|Police Dog Technical School of the Ministry of Public Security, Shenyang, 110034, China.
authors.forename: Jian-Yu
authors.initials: JY
authors.lastname: Liu
authors.affiliation: Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun, 130112, China.
authors.forename: Yan-zhu
authors.initials: YZ
authors.lastname: Zhu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Yan-Fei
authors.initials: YF
authors.lastname: Li
mesh_terms: D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism*; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D002712:Chlorides / Q000633:toxicity*; D045304:Cytochromes c / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D013154:Spleen / Q000166:cytology; D051028:bcl-2-Associated X Protein / Q000235:genetics
chemical_list: D017607:Aluminum Compounds; C494828:Bax protein, rat; D002712:Chlorides; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D000077410:Aluminum Chloride; D045304:Cytochromes c; D053148:Caspase 3; D053453:Caspase 9
keywords: aluminum; apoptosis; caspase; mitochondria; rat spleen lymphocytes