title: Comparative studies on polyphenolic composition, antioxidant and antimicrobial activities of Schisandra chinensis leaves and fruits.
abstract: The aim of this paper was to evaluate the antioxidant and antimicrobial activities and the polyphenolic content of Schisandra chinensis (Turcz.) Baill. leaves and fruits. The leaves are an important source of flavonoids (35.10 ± 1.23 mg RE/g plant material). Qualitative and quantitative analyses of the polyphenolic compounds were achieved using a HPLC-UV-MS method. The main flavonoid from the leaves was isoquercitrin (2486.18 ± 5.72 μg/g plant material), followed by quercitrin (1645.14 ± 2.12 μg/g plant material). Regarding the fruit composition, the dominant compound there was rutin (13.02 ± 0.21 μg/g plant material), but comparing with the leaves, fruits can be considered a poor source of phenolic compounds. The antioxidant activity was evaluated by DPPH, TEAC, hemoglobin ascorbate peroxidase activity inhibition (HAPX), inhibition of lipid peroxidation catalyzed by cytochrome c and EPR spectroscopic assays, revealing a better antioxidant activity for the S. chinensis leaves extract. In the antimicrobial assay, S. chinensis leaves extract showed efficient activities against the targeted bacteria, being more active than the fruits extract. The results suggest the leaves of S. chinensis as a valuable source of antioxidant compounds with significant antioxidant activity.
authors.affiliation: Department of Pharmaceutical Botany, Iuliu Hațieganu University of Medicine and Pharmacy, 12 I. Creangă Street, Cluj-Napoca 400010, Romania. mocan.andrei@umfcluj.ro.
authors.forename: Andrei
authors.initials: A
authors.lastname: Mocan
authors.affiliation: Department of Pharmaceutical Botany, Iuliu Hațieganu University of Medicine and Pharmacy, 12 I. Creangă Street, Cluj-Napoca 400010, Romania. gcrisan@umfcluj.ro.
authors.forename: Gianina
authors.initials: G
authors.lastname: Crișan
authors.affiliation: Department of Pharmaceutical Technology and Biopharmaceutics, Iuliu Hațieganu University of Medicine and Pharmacy, 12 I. Creangă Street, Cluj-Napoca 400010, Romania. laurian.vlase@umfcluj.ro.
authors.forename: Laurian
authors.initials: L
authors.lastname: Vlase
authors.affiliation: Department of Organic Chemistry, Iuliu Hațieganu University of Medicine and Pharmacy, 12 I. Creangă Street, Cluj- Napoca 400010, Romania. ocrisan@umfcluj.ro.
authors.forename: Ovidiu
authors.initials: O
authors.lastname: Crișan
authors.affiliation: Department of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manăştur Street, Cluj-Napoca 400372, Romania. dan.vodnar@usamvcluj.ro.
authors.forename: Dan Cristian
authors.initials: DC
authors.lastname: Vodnar
authors.affiliation: Department of Physics of Nanostructured Materials, National Institute for Research and Development of Isotopic and Molecular Technologies 65-103, Donath Street, Cluj-Napoca 400010, Romania. oana.raita@itim-cj.ro.
authors.forename: Oana
authors.initials: O
authors.lastname: Raita
authors.affiliation: Department of Pharmaceutical Technology and Biopharmaceutics, Iuliu Hațieganu University of Medicine and Pharmacy, 12 I. Creangă Street, Cluj-Napoca 400010, Romania. Gheldiu.Ana@umfcluj.ro.
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Gheldiu
authors.affiliation: Department of Pharmacognosy, Iuliu Hațieganu University of Medicine and Pharmacy, 12 I. Creangă Street, Cluj-Napoca 400010, Romania. atoiu@umfcluj.ro.
authors.forename: Anca
authors.initials: A
authors.lastname: Toiu
authors.affiliation: Department of Analytical Chemistry and Instrumental Analysis, Iuliu Hațieganu University of Medicine and Pharmacy, 4 L. Pasteur Street, Cluj-Napoca 400010, Romania. roprean@umfcluj.ro.
authors.forename: Radu
authors.initials: R
authors.lastname: Oprean
authors.affiliation: Family Medicine, Department M3 Clinical Sciences Internal Medicine, University of Medicine and Pharmacy, 38 G. Marinescu Street, Târgu Mures 540139, Romania. ioan.tilea@umftgm.ro.
authors.forename: Ioan
authors.initials: I
authors.lastname: Tilea
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004578:Electron Spin Resonance Spectroscopy; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D018515:Plant Leaves / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry*; D032141:Schisandra / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D059808:Polyphenols