title: [Molecular identification of Manis pentadactyla using DNA barcoding].
abstract: The COI gene as DNA barcode was used to identify the Manis pentadactyla and its adulterants in order to provide a scientific basis for the molecular identification of M. pentadactyla. Genomic DNA was extracted from experimental samples using the DNA extraction kit. The COI genes were amplified using polymerase chain reaction (PCR) and sequenced bi-directionally. Obtained sequences were assembled using the CodonCode Aligner. The neighbor-joining (NJ) tree was constructed by MEGA 6.0. The results indicated that COI sequences were successfully amplified and NJ trees results indicated that M. pentadactyla and its adulterants can be easily identification. Therefore, the COI gene is an efficient barcode for identification of M. pentadactyla and its adulterants,which will provide a new technique for the market supervision.
authors.forename: Jing
authors.initials: J
authors.lastname: Jia
authors.forename: Hong-Yin
authors.initials: HY
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Yao
authors.forename: Qi-Ni
authors.initials: QN
authors.lastname: Qian
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D004340:Drug Contamination / Q000517:prevention & control; D003576:Electron Transport Complex IV / Q000235:genetics; D008322:Mammals / Q000145:classification* / Q000235:genetics*; D008516:Medicine, Chinese Traditional; D008969:Molecular Sequence Data; D010802:Phylogeny; D011786:Quality Control; D012756:Sheep; D013552:Swine
chemical_list: C038862:cytochrome o oxidase; D003576:Electron Transport Complex IV