title: Inflammatory markers, oxidative stress, and antioxidant capacity in healthy allo-HSCT donors during hematopoietic stem cell mobilization.
abstract: The aim of this study is to investigate the impact of mobilization with granulocyte colony stimulating factor (G-CSF) and apheresis procedure on inflammatory and oxidative stress markers, and antioxidant capacity in healthy allo-HSCT donors. The study was conducted in the Stem Cell Transplantation Unit of Gazi University Hospital between October 2010 and March 2011, and 25 consecutive allo-HSCT donors were included. The alteration in the serum levels of iron, iron binding capacity, albumin, ferritin, IL-6, hs-CRP, TAC, MDA, and AOPP were determined at five different time points. (1) Prior to the first dose of G-CSF (T0), (2) preapheresis (on the fourth day of G-CSF before the apeheresis procedure) (T1), (3) immediately postapheresis (T2), (4) 24 h postapheresis (T3), and (5) a week after apheresis (T4). Serum ferritin levels increased steadily after administration of G-CSF and remained high up toT4. Both serum IL-6 and hs-CRP levels began to increase in the T1 sampling and reached to a maximum level at T3 and decreased even below the basal levels at T4. Serum AOPP levels decreased at preapheresis and postapheresis time points, while they increased at T3 and T4 samples. Serum MDA levels decreased at T1, T2, T3, and T4 samples. Serum TAC increased significantly and steadily at all time points post G-CSF. In conclusion; mobilization with G-CSF and apheresis caused a transient inflammatory reaction and a protein limited oxidative stress in healthy allo-HCT donors.
authors.affiliation: Department of Biochemistry, Gazi University Faculty of Medicine, Ankara, Turkey.
authors.forename: Çiğdem
authors.initials: Ç
authors.lastname: İlhan
authors.affiliation: Department of Hematology, Gazi University Faculty of Medicine, Ankara, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Suyanı
authors.affiliation: Department of Hematology, Gazi University Faculty of Medicine, Ankara, Turkey.
authors.forename: Gülsan Türköz
authors.initials: GT
authors.lastname: Sucak
authors.affiliation: Department of Biochemistry, Gazi University Faculty of Medicine, Ankara, Turkey.
authors.forename: Özge Tuğçe
authors.initials: ÖT
authors.lastname: Paşaoğlu
authors.affiliation: Department of Hematology, Gazi University Faculty of Medicine, Ankara, Turkey.
authors.forename: Şahika Zeynep
authors.initials: ŞZ
authors.lastname: Akı
authors.affiliation: Department of Biochemistry, Gazi University Faculty of Medicine, Ankara, Turkey.
authors.forename: Hatice
authors.initials: H
authors.lastname: Paşaoğlu
mesh_terms: D000328:Adult; D062107:Advanced Oxidation Protein Products / Q000097:blood; D000368:Aged; D000418:Albumins / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism*; D001781:Blood Component Removal; D002097:C-Reactive Protein / Q000378:metabolism; D005260:Female; D005293:Ferritins / Q000097:blood; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D019650:Hematopoietic Stem Cell Mobilization; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007249:Inflammation / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood; D007501:Iron / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D011446:Prospective Studies; D012709:Serum Albumin / Q000378:metabolism; D014184:Transplantation, Homologous; D055815:Young Adult
chemical_list: D062107:Advanced Oxidation Protein Products; D000418:Albumins; D018952:Antigens, CD34; D000975:Antioxidants; D015850:Interleukin-6; D012709:Serum Albumin; D016179:Granulocyte Colony-Stimulating Factor; D008315:Malondialdehyde; D002097:C-Reactive Protein; D005293:Ferritins; D007501:Iron; D010100:Oxygen
keywords: antioxidant capacity; donor; hematopoietic stem cell transplantation; mobilization; oxidative stress