title: Conjugated-protein mimics with molecularly imprinted reconstructible and transformable regions that are assembled using space-filling prosthetic groups.
abstract: Conjugated-protein mimics were obtained using a new molecular imprinting strategy combined with post-imprinting modifications. An antibiotic was employed as a model template molecule, and a polymerizable template molecule was designed, which was composed of the antibiotic and two different prosthetic groups attached through a disulfide bond and Schiff base formation. After co-polymerization with a cross-linker, the template molecule was removed together with the prosthetic groups, yielding the apo-type scaffold. Through conjugation of the two different prosthetic groups at pre-determined positions within the apo-type scaffold, the apo cavity was transformed into a functionalized holo cavity, which enables the on/off switching of the molecular recognition ability, signal transduction activity for binding events, and photoresponsive activity.
authors.affiliation: Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501 (Japan). takeuchi@gold.kobe-u.ac.jp.
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Takuya
authors.initials: T
authors.lastname: Mori
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kuwahara
authors.forename: Takeo
authors.initials: T
authors.lastname: Ohta
authors.forename: Azusa
authors.initials: A
authors.lastname: Oshita
authors.forename: Hirobumi
authors.initials: H
authors.lastname: Sunayama
authors.forename: Yukiya
authors.initials: Y
authors.lastname: Kitayama
authors.forename: Tooru
authors.initials: T
authors.lastname: Ooya
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry*; D054802:Molecular Imprinting; D018716:Molecular Mimicry; D015394:Molecular Structure; D011506:Proteins / Q000737:chemistry*; D012545:Schiff Bases / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D004220:Disulfides; D011506:Proteins; D012545:Schiff Bases
keywords: imprinting; molecular recognition; polymers; protein mimics