title: Serum metabolite signatures of type 2 diabetes mellitus complications.
abstract: A number of metabolic conditions, including hypoglycemia, high blood pressure (HBP), dyslipidemia, nerve damage and amputation, and vision problems, occur as a result of uncontrolled blood glucose levels over a prolonged period of time. The different components of diabetic complications are not independent but rather interdependent of each other, rendering the disease difficult to diagnose and control. The underlying pathogenesis of those components cannot be easily elucidated because of the heterogeneous, polygenic, and multifactorial nature of the disease. Metabonomics offers a snapshot of distinct biochemical variations that may reflect the unique metabolic phenotype under pathophysiological conditions. Here we report a mass-spectrometry-based metabonomic study designed to identify the distinct metabolic changes associated with several complications of type 2 diabetes mellitus (T2DM). The 292 patients recruited in the study were divided into five groups, including T2DM with HBP, T2DM with nonalcoholic fatty liver disease (NAFLD), T2DM with HBP and NAFLD, T2DM with HBP and coronary heart disease (CHD), and T2DM with HBP, NAFLD, and CHD. Serum differential metabolites were identified in each group of T2DM complication, mainly involving bile acid, fatty acid, amino acid, lipid, carbohydrate, steroids metabolism, and tricarboxylic acids cycle. These broad-spectrum metabolic changes emphasize the complex abnormalities present among these complications with elevated blood glucose levels, providing a novel strategy for stratifying patients with T2DM complications using blood-based metabolite markers.
authors.affiliation: Center of Chinese Medical Therapy and Systems Biology, Shanghai University of Traditional Chinese Medicine , 1200 Cailun Road, Shanghai 201203, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.forename: Guoxiang
authors.initials: G
authors.lastname: Xie
authors.forename: Yan
authors.initials: Y
authors.lastname: Ni
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Huafeng
authors.initials: H
authors.lastname: Wei
authors.forename: Wei
authors.initials: W
authors.lastname: Jia
authors.forename: Guang
authors.initials: G
authors.lastname: Ji
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000886:Anthropometry; D015415:Biomarkers / Q000097:blood*; D015992:Body Mass Index; D002681:China; D002784:Cholesterol / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D003327:Coronary Disease / Q000097:blood* / Q000209:etiology; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications*; D006801:Humans; D006973:Hypertension / Q000097:blood* / Q000209:etiology; D008075:Lipoproteins, HDL / Q000097:blood; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods*; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood* / Q000209:etiology; D014280:Triglycerides / Q000097:blood
chemical_list: D015415:Biomarkers; D008075:Lipoproteins, HDL; D014280:Triglycerides; D002784:Cholesterol; D000410:Alanine Transaminase
keywords: complication; metabolic signatures; metabonomic profiling; serum; type-2 diabetes mellitus