title: Evaluation of MALDI-TOF mass spectrometry for identification of anaerobic bacteria.
abstract: In this study MALDI-TOF MS was evaluated in the identification of anaerobic bacteria comparing it with Rapid ID 32A system. Discrepancies were solved by 16S r-RNA gene sequencing. At the species level MALDI-TOF MS identified 94.82% and Rapid ID 32A 86.67%, showing the superiority of MALDI-TOF MS to conventional methods.
authors.affiliation: Servicio de Microbiología, Complejo Hospitalario Universitario A Coruña, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Barba
authors.affiliation: Servicio de Microbiología, Complejo Hospitalario Universitario A Coruña, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Fernández
authors.affiliation: Servicio de Microbiología, Complejo Hospitalario Universitario A Coruña, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Oviaño
authors.affiliation: Servicio de Microbiología, Complejo Hospitalario Universitario A Coruña, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Fernández
authors.affiliation: Servicio de Microbiología, Complejo Hospitalario Universitario A Coruña, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Velasco
authors.affiliation: Servicio de Microbiología, Complejo Hospitalario Universitario A Coruña, Spain. Electronic address: german.bou.arevalo@sergas.es.
authors.forename: G
authors.initials: G
authors.lastname: Bou
mesh_terms: D001421:Bacteria, Anaerobic / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology*; D001431:Bacteriological Techniques / Q000379:methods*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA; Anaerobe; MALDI-TOF; Rapid ID 32 A