title: In vitro proliferation and differentiation potential of bone marrow-derived mesenchymal stem cells from ovariectomized rats.
abstract: Bone marrow-derived mesenchymal stem cells (BMMSCs) from the patients suffering from age-related osteoporosis were found to have numerous degeneration, such as decreased growth rate, impaired capacity of differentiating into local tissue, and repressed telomerase activity. However, it is not clear whether post-menopausal osteoporotic bone is either subject to such decline in cellular function. In the present study, bone marrow cells were harvested from ovariectomized (OVX) and Sham rats and cultured in vitro at 3 months post-surgery. MTT assay indicated that the proliferation potential of (OVX)BMMSCs was always higher than that of (Sham)BMMSCs, no matter cultured in basic, osteoblastic or adipogenic medium. Alkaline phosphatase activity assay, Alizarin red S staining, Oil red O staining and real-time RT-PCR analysis further demonstrated that bilateral ovariectomization positively influenced the osteoblastic and adipogenic differentiation potential of BMMSCs, this action may be partly mediated through up-regulation of osteoblastic special markers core binding factor a1, collagen type I and low-density lipoprotein receptor-related protein 5, as well as adipogenic special markers peroxisome proliferators activated receptor gamma, CCAAT/enhancer binding protein alpha and adipocyte lipid-binding protein 2. These results may hold great promise for using post-menopausal osteoporotic bone as an attractive autologous marrow source for tissue engineering and cell-based therapies.
authors.affiliation: Department of Stomatology, The First Hospital of Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Stomatology, The First Hospital of Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: Department of Stomatology, The First Hospital of Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Nie
authors.affiliation: Department of Stomatology, The First Hospital of Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Lian
authors.affiliation: Department of Otorhinolaryngology, The First Hospital of Shanxi Medical University, Taiyuan 030001, China. Electronic address: yinuodoc@163.com.
authors.forename: Binquan
authors.initials: B
authors.lastname: Wang
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D059630:Mesenchymal Stem Cells; D010012:Osteogenesis / Q000235:genetics*; D010024:Osteoporosis; D010052:Ovariectomy; D051381:Rats; D023822:Tissue Engineering
keywords: Cell culture; Osteoporosis; Stem cells; Tissue engineering