title: A surface acoustic wave bio-electronic nose for detection of volatile odorant molecules.
abstract: In this work, a "bio-electronic nose" for vapour phase detection of odorant molecules based on surface acoustic wave (SAW) resonators is presented. The biosensor system is composed of an array of five SAW resonators coated with three types of odorant-binding proteins (OBPs): the wild-type OBP from bovine (wtbOBP), a double-mutant of the OBP from bovine (dmbOBP), and the wild-type OBP from pig (wtpOBP). High resolution deposition of OBPs onto the active area of SAW resonators was implemented through laser-induced forward transfer (LIFT). The resonant frequency shifts of the SAW resonators after the deposition of the biomolecules confirmed the immobilisation of the proteins onto the Al/Au inter-digital transducers (IDTs). In addition, a low increase of insertion losses with a limited degradation of Q-factors is reported. The "bio-electronic nose" fabricated by LIFT is tested in nitrogen upon exposure to separated concentrations of R-(-)-1-octen-3-ol (octenol) and R-(-)-carvone (carvone) vapours. The "bio-electronic nose" showed low detection limits for the tested compounds (i.e. 0.48 ppm for the detection of octenol, and 0.74 ppm for the detection of carvone). In addition, the bio-sensing system was able to discriminate the octenol molecules from the carvone molecules, making it pertinent for the assessment of food contamination by moulds, or for the evaluation of indoor air quality in buildings.
authors.affiliation: "O.M. Corbino" Institute of Acoustics and Sensors, Italian National Research Council, Via del Fosso del Cavaliere 100, 00133 Rome, Italy. Electronic address: fabio.dp@idasc.cnr.it.
authors.forename: F
authors.initials: F
authors.lastname: Di Pietrantonio
authors.affiliation: "O.M. Corbino" Institute of Acoustics and Sensors, Italian National Research Council, Via del Fosso del Cavaliere 100, 00133 Rome, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Benetti
authors.affiliation: "O.M. Corbino" Institute of Acoustics and Sensors, Italian National Research Council, Via del Fosso del Cavaliere 100, 00133 Rome, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Cannatà
authors.affiliation: Institute for Photonics and Nanotechnologies, Italian National Research Council, Via del Cineto Romano 42, 00156 Rome, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Verona
authors.affiliation: Departament de Física Aplicada i Òptica, Universitat de Barcelona, Martí i Franquès 1, E-08028 Barcelona, Spain; National Institute for Lasers, Plasma, and Radiation Physics, Magurele, Atomistilor 409, P.O. Box MG 16, 077125 Bucharest, Romania.
authors.forename: A
authors.initials: A
authors.lastname: Palla-Papavlu
authors.affiliation: Departament de Física Aplicada i Òptica, Universitat de Barcelona, Martí i Franquès 1, E-08028 Barcelona, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Fernández-Pradas
authors.affiliation: Departament de Física Aplicada i Òptica, Universitat de Barcelona, Martí i Franquès 1, E-08028 Barcelona, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Serra
authors.affiliation: Institute of Protein Biochemistry, Italian National Research Council, Via Pietro Castellino 111, 80131 Naples, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Staiano
authors.affiliation: Institute of Protein Biochemistry, Italian National Research Council, Via Pietro Castellino 111, 80131 Naples, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Varriale
authors.affiliation: Institute of Protein Biochemistry, Italian National Research Council, Via Pietro Castellino 111, 80131 Naples, Italy.
authors.forename: S
authors.initials: S
authors.lastname: D'Auria
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D062609:Electronic Nose; D057230:Limit of Detection; D009812:Odorants / Q000032:analysis*; D018035:Receptors, Odorant / Q000737:chemistry*; D013016:Sound; D013552:Swine
chemical_list: D018035:Receptors, Odorant; C045648:odorant-binding protein
keywords: Bio-electronic nose; Laser-induced forward transfer; Odorant-binding protein; Surface acoustic waves biosensors