title: First report of isolation of Mycobacterium canariasense from hospital water supplies.
abstract: Mycobacterium canariasense was first isolated as a novel species in 2004 from clinical specimens in Spain. Since then there have only been a few additional reports from Spain, the USA, and Lebanon on the isolation of this rare species from clinical specimens. We herein present the first report on isolation of this organism from hospital water, which provides evidence for determining the natural habitat of this rare species. The water samples were collected from hospital departments and cultured on Löwenstein-Jensen and Sauton's media. The isolates, i.e. WP5, WP20, and AW2-3, were subjected to identification by conventional and molecular tests including sequencing analysis of 16S rRNA. The water isolates revealed phenotypic and molecular features consistent with M. canariasense including a genus-specific amplicon of the hsp65 gene and 100% similarities with those of M. canariasense CIP: 107998(T) 16S rRNA gene sequences. The current report might be of value in tracing the probable source of infection in patients.
authors.affiliation: From the Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences.
authors.forename: Davood
authors.initials: D
authors.lastname: Azadi
authors.forename: Ramin
authors.initials: R
authors.lastname: Dibaj
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Pourchangiz
authors.forename: Abass
authors.initials: A
authors.lastname: Daei-Naser
authors.forename: Hasan
authors.initials: H
authors.lastname: Shojaei
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D006761:Hospitals; D007492:Iran; D009161:Mycobacterium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology; D014881:Water Supply / Q000032:analysis* / Q000592:standards*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA; Mycobacterium canariasense; hsp65; nosocomial infection