title: Propagation of rhinovirus-C strains in human airway epithelial cells differentiated at air-liquid interface.
abstract: Rhinovirus-C (RV-C) were discovered recently using molecular methods. Classical methods failed to detect them since they could not grow in standard cell culture. The complete genome sequences of several RV-C strains are now available but there is little information about their biological characteristics. HRV-C were first grown in organ culture, and more recently, we developed a system for culturing RV-C strains in differentiated epithelial cells of human airway at air-liquid interface (ALI). These cultures supported efficient replication of RV-C strains as determined by quantitative RT-PCR. This system has enabled study of the biological characteristics of RV-C strains, including quantitative research.
authors.affiliation: Department of Pediatrics, School of Medicine and Public Health, University of Wisconsin, Madison, WI, USA, sashraf@atcc.org.
authors.forename: Shamaila
authors.initials: S
authors.lastname: Ashraf
authors.forename: Rebecca
authors.initials: R
authors.lastname: Brockman-Schneider
authors.forename: James E
authors.initials: JE
authors.lastname: Gern
mesh_terms: D001980:Bronchi / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000821:virology*; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012229:Rhinovirus / Q000235:genetics / Q000254:growth & development*