title: Antioxidative properties of ascorbigen in using multiple antioxidant assays.
abstract: The antioxidative properties of ascorbigen, one of the major indole-derived compounds of Brassica vegetables, were systematically evaluated using multiple assay systems with comparison to the well-known antioxidants ascorbic acid and Trolox. We first performed assays using model radicals, DPPH radical, galvinoxyl radical, and ABTS radical cation (ABTS(•+)). Ascorbigen showed stronger activity than that of ascorbic acid in the ABTS(•+)-scavenging assay but showed no activity in the DPPH radical- and galvinoxyl radical-scavenging assays. In the ABTS(•+)-scavenging assay, the indole moiety of ascorbigen contributed to scavenging of the radicals to produce indole-3-aldehyde as one of the final reaction products. The activity of ascorbigen was then evaluated by an oxygen radical absorbance capacity assay and an oxidative hemolysis inhibition assay using physiologically relevant peroxyl radicals, AAPH-derived radicals. Ascorbigen showed much stronger antioxidant activity than did ascorbic acid and Trolox. Therefore, antioxidant activity of ascorbigen might be more beneficial than has been thought for daily health care.
authors.affiliation: a Faculty of Life and Environmental Sciences , Prefectural University of Hiroshima , Shobara , Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Tai
authors.forename: Kanako
authors.initials: K
authors.lastname: Fukunaga
authors.forename: Asako
authors.initials: A
authors.lastname: Ohno
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Ito
mesh_terms: D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D001681:Biological Assay; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D005609:Free Radicals / Q000737:chemistry; D007211:Indoles / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals; D007211:Indoles; C053152:ascorbigen; D001205:Ascorbic Acid
keywords: ABTS radical cation; ORAC; antioxidant; ascorbigen; oxidative hemolysis inhibition assay