title: [Study on anti-oxidative effect of extracts from Cichorium endivia on HepG2 cells and its mechanism].
abstract: OBJECTIVE
  To investigate the protective effect of extracts from Cichorium endivia (CEE) in H2O2-induced HepG2 cell oxidative stress injury, and explore the antioxidant mechanism of CEE in HepG2 cells.
  
  
  METHODS
  The viability of H2O2-induced HepG2 cells and the intracellular ROS level were measured by MTT assay and DCFH-DA fluorescence staining assay. The antioxidant-response element (ARE)-Luciferase activity was tested in HepG2 cells stably transected by ARE reporter gene. The fluorescence quantitative RT-PCR was adopted to determine the mRNA expressions of genes containing ARE sequence in HepG2 cells.
  
  
  RESULTS
  The cell viability reduced, while the ROS level increased after HepG2 cells were treated by H2O2. Different concentrations of CEE could be added to significantly improve the above results. After HepG2 cells transected by ARE reporter gene were treated with different concentrations of CEE, the intracellular ARE activity could increase in a concentration-dependent manner. In addition, the mRNA expressions of regulatory genesGCLC, GCLM and HMOX-1 containing ARE sequence in HepG2 cells were up-regulated in a concentration-dependent manner by CEE.
  
  
  CONCLUSIONS
  CEE inhibited the H2O2-injured HepG2 cells by reducing the ROS level. CEE's antioxidant mechanism for HepG2 cells may be closely related to the antioxidant defense system associated with its effect of activating Nrf2-ARE pathway in HepG2 cells.
authors.forename: Chao-Jie
authors.initials: CJ
authors.lastname: Chen
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Zhan
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Wei
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Jin
authors.forename: Hai-Lin
authors.initials: HL
authors.lastname: Qin
authors.forename: Ai-Ping
authors.initials: AP
authors.lastname: Wang
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification* / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D020218:Response Elements / Q000235:genetics
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide