title: Analysis of potential migrants from plastic materials in milk by liquid chromatography-mass spectrometry with liquid-liquid extraction and low-temperature purification.
abstract: A simple and fast analytical method was developed for the determination of six UV stabilizers (Cyasorb UV-1164, Tinuvin P, Tinuvin 234, Tinuvin 326, Tinuvin 327, and Tinuvin 1577) and five antioxidants (Irgafos 168, Irganox 1010, Irganox 3114, Irganox 3790, and Irganox 565) in milk. For sample preparation liquid-liquid extraction with low-temperature purification combined with centrifugation was used to remove fats, proteins, and sugars. After the cleanup step, the sample was analyzed with high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). External standard and matrix calibrations were tested. External calibration proved to be acceptable for Tinuvin P, Tinuvin 234, Tinuvin 326, Tinuvin 327, Irganox 3114, and Irganox 3790. The method was successfully validated with matrix calibration for all compounds. Method detection limits were between 0.25 and 10 μg/kg. Accuracies ranged from 93 to 109%, and intraday precisions were <13%.
authors.affiliation: Joint Research and Training Laboratory on Separation Techniques (EKOL), Eötvös Loránd University , 1/A Pázmány Péter sétány, Budapest 1117, Hungary.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Bodai
authors.forename: Bálint Sámuel
authors.initials: BS
authors.lastname: Szabó
authors.forename: Márton
authors.initials: M
authors.lastname: Novák
authors.forename: Susanne
authors.initials: S
authors.lastname: Hámori
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Nyiri
authors.forename: Tamás
authors.initials: T
authors.lastname: Rikker
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Eke
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003080:Cold Temperature; D005506:Food Contamination / Q000032:analysis*; D018857:Food Packaging; D059625:Liquid-Liquid Extraction / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D010969:Plastics / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D010969:Plastics
keywords: LC-MS/MS; UV stabilizers; antioxidants; liquid−liquid extraction with low-temperature purification; migration; milk; polymer additives