title: In vitro evaluation of antimicrobial activity of different Gutta-percha points and calcium hydroxide pastes.
abstract: OBJECTIVE
  To evaluate the antimicrobial activity of different compositions of Gutta-percha points and calcium hydroxide (Ca(OH)2) pastes, used in endodontic therapy.
  
  
  METHODS
  The evaluated material consisted of Gutta-percha points containing Ca(OH)2, Gutta-percha points containing chlorhexidine (Chx), conventional Gutta-percha points and Ca(OH)2 pastes. Antimicrobial properties of Chx and CaOH paste are compared with CaOH points. Antimicrobial tests included three species of microorganisms: Escherichia coli (ATCC 25923), Staphylococcus aureus (ATCC 25922) Pseudomonas aeruginosa (ATCC BAA-427), the agar disc diffusion method was employed. The plates were kept at room temperature for 2 h for prediffusion and then incubated at 37°C for 24 h. Zones of inhibition were measured.
  
  
  CONCLUSIONS
  All microbial species used in the study were inhibited by the Gutta-percha points containing Chx and by the Ca(OH)2 pastes, no antimicrobial activity was observed for the other groups (conventional Gutta-percha and Ca(OH)2 group).
authors.affiliation: Department of Conservative Dentistry and Endodontics, Yamuna Institute of Dental Sciences and Research, Gadholi, Yamunanagar, Haryana, India.
authors.forename: Ashu
authors.initials: A
authors.lastname: Jhamb
authors.affiliation: Department of Conservative Dentistry and Endodontics, KLE Dental College, Belgaum, Karnataka, India.
authors.forename: Vishwajit Rampratap
authors.initials: VR
authors.lastname: Chaurasia
authors.affiliation: Department of Conservative Dentistry and Endodontics, KLE Dental College, Belgaum, Karnataka, India.
authors.forename: Vinay Kumar S
authors.initials: VK
authors.lastname: Masamatti
authors.affiliation: Department of Conservative Dentistry and Endodontics, Indraprastha Dental College, Ghaziyabad, Uttar Pradesh, India.
authors.forename: Jai Hans
authors.initials: JH
authors.lastname: Agarwal
authors.affiliation: Department of Periodontology, Bhabha College of Dental Science, Bhopal, Madya Pradesh, India.
authors.forename: Samarth
authors.initials: S
authors.lastname: Tiwari
authors.affiliation: Department of Oral and Maxillofacial Pathology, DJ College of Dental Sciences and Research, Modinagar, Uttar Pradesh, India.
authors.forename: Divya
authors.initials: D
authors.lastname: Nair
keywords: Calcium hydroxide; Gutta-percha; chlorhexidine