title: First evidence of mariner-like transposons in the genome of the marine microalga Amphora acutiuscula (Bacillariophyta).
abstract: Mariner-like elements (MLEs) are transposable elements able to move in the host genomes by a "cut and paste" mechanism. They have been found in numerous organisms. We succeeded in amplifying complete and truncated MLEs in the marine diatom Amphora acutiuscula. Full-length MLEs of 2,100bp delimited by imperfect Terminal Inverted Repeats revealed an intact Open Reading Frame, suggesting that the MLEs could be active. The DNA binding domain of the corresponding putative transposase could have two Helix-Turn-Helix and a Nuclear Location Site motifs, and its catalytic domain includes a particular triad of aspartic acids DD43D not previously reported. The number of copies was estimated to be 38, including approximately 20 full-length elements. Phylogenetic analysis shows that these peculiar MLEs differ from plant and other stramenopile MLEs and that they could constitute a new sub-family of Tc1-mariner elements.
authors.affiliation: PRES LUNAM université, Université du Maine, Laboratoire Mer, Molécules, Santé (EA 2160), avenue Olivier Messiaen, 72085 Le Mans cedex 9, France.
authors.forename: Duc Hung
authors.initials: DH
authors.lastname: Nguyen
authors.affiliation: PRES LUNAM université, Université du Maine, Laboratoire Mer, Molécules, Santé (EA 2160), avenue Olivier Messiaen, 72085 Le Mans cedex 9, France.
authors.forename: Dorothée
authors.initials: D
authors.lastname: Hermann
authors.affiliation: PRES LUNAM université, Université du Maine, Laboratoire Mer, Molécules, Santé (EA 2160), avenue Olivier Messiaen, 72085 Le Mans cedex 9, France.
authors.forename: Aurore
authors.initials: A
authors.lastname: Caruso
authors.affiliation: PRES LUNAM université, Université du Maine, Laboratoire Mer, Molécules, Santé (EA 2160), avenue Olivier Messiaen, 72085 Le Mans cedex 9, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Tastard
authors.affiliation: PRES LUNAM université, Université du Maine, Laboratoire Mer, Molécules, Santé (EA 2160), avenue Olivier Messiaen, 72085 Le Mans cedex 9, France.
authors.forename: Justine
authors.initials: J
authors.lastname: Marchand
authors.affiliation: Laboratoire Evolution Génome et Spéciation, CNRS UPR 9034, avenue de la terrasse, bâtiment 13, BP 1, 91198 Gif-sur-Yvette, France.
authors.forename: Jacques-Déric
authors.initials: JD
authors.lastname: Rouault
authors.affiliation: PRES LUNAM université, Université du Maine, Laboratoire Mer, Molécules, Santé (EA 2160), avenue Olivier Messiaen, 72085 Le Mans cedex 9, France; UMR BOREA « Biologie des Organismes et Ecosystèmes Aquatiques », Muséum National d'Histoire Naturelle (MNHN), CP 26, 43 rue Cuvier, 75231 Paris Cedex 05, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Denis
authors.affiliation: PRES LUNAM université, Université du Maine, Laboratoire Mer, Molécules, Santé (EA 2160), avenue Olivier Messiaen, 72085 Le Mans cedex 9, France.
authors.forename: Stanislas
authors.initials: S
authors.lastname: Thiriet-Rupert
authors.affiliation: PRES LUNAM université, Université du Maine, Laboratoire Mer, Molécules, Santé (EA 2160), avenue Olivier Messiaen, 72085 Le Mans cedex 9, France. Electronic address: ncasse@univ-lemans.fr.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Casse
authors.affiliation: PRES LUNAM université, Université du Maine, Laboratoire Mer, Molécules, Santé (EA 2160), avenue Olivier Messiaen, 72085 Le Mans cedex 9, France. Electronic address: annick.manceau@univ-lemans.fr.
authors.forename: Annick
authors.initials: A
authors.lastname: Morant-Manceau
mesh_terms: D000595:Amino Acid Sequence; D059001:Aquatic Organisms / Q000235:genetics; D016000:Cluster Analysis; D004251:DNA Transposable Elements; D017377:Diatoms / Q000235:genetics*; D016678:Genome; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D019895:Transposases / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D019895:Transposases
keywords: DNA transposon; Diatom; mariner-like element; transposable elements.