title: Evaluation of the Carba NP test for carbapenemase detection.
abstract: The Carba NP test was evaluated against a panel of 61 carbapenemase-producing bacterial species (15 producing class A carbapenemases, 15 producing class D carbapenemases, and 31 producing metallo-β-lactamases) and against 111 isolates with non-wild-type carbapenem susceptibility but not producing carbapenemase. Carbapenemase production was verified by PCR and UV-spectrophotometric measurement of imipenem hydrolysis. No false positives were seen, but there were consistent problems with the detection of OXA-48-like enzymes and also some rarer class A enzymes.
authors.affiliation: Antimicrobial Resistance Unit, National Institute for Health and Welfare, Turku, Finland monica.osterblad@thl.fi.
authors.forename: Monica
authors.initials: M
authors.lastname: Osterblad
authors.affiliation: Antimicrobial Resistance Unit, National Institute for Health and Welfare, Turku, Finland Dept. of Medical Microbiology and Immunology, University of Turku, Turku, Finland.
authors.forename: Antti J
authors.initials: AJ
authors.lastname: Hakanen
authors.affiliation: Antimicrobial Resistance Unit, National Institute for Health and Welfare, Turku, Finland.
authors.forename: Jari
authors.initials: J
authors.lastname: Jalava
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016971:Citrobacter freundii / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D021902:Enterobacter aerogenes / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D016972:Enterobacter cloacae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D057075:Enzyme Assays / Q000592:standards*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D006868:Hydrolysis; D015378:Imipenem / Q000378:metabolism* / Q000494:pharmacology; D041121:Klebsiella oxytoca / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015378:Imipenem; D001618:beta-Lactamases; C063912:carbapenemase