title: Differential temporal expression profiles of heat shock protein genes in Drosophila melanogaster (Diptera: Drosophilidae) under ultraviolet A radiation stress.
abstract: Solar UV radiation is indispensable for certain behaviors of many organisms. Nevertheless, UV-A might be expected to stress insects that possess intensive positive taxis toward UV-A light. To avoid stress hazards, organisms generally exhibit the upregulation of heat shock proteins (Hsps) expression. To gain a better understanding of the roles of the different Hsps in response to UV-A stress in the diurnal phototactic fly Drosophila melanogaster Meigen, 1830 (Diptera: Drosophilidae), we tested the temporal expression patterns of 11 DmHsps following UV-A radiation. The results indicated that each DmHsp had a differential temporal expression profile under UV-A radiation stress. Potential transcription factor-binding motifs in the promoter regions of strongly inducible DmHsps were identified; results showed these transcription factor-binding motifs were highly homologous to binding sites that have been identified for transcription factors associated with UV radiation stimuli. So DmHsps might act in a coordinated and cooperative manner at the transcriptional level to counteract UV-A radiation-based stress.
authors.affiliation: Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, Huazhong Agricultural University, ShiziMountain Rd., Wuhan 430070, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Wang
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Zhou
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Zhu
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Ma
authors.forename: Chao-Liang
authors.initials: CL
authors.lastname: Lei
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects*; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D029721:Drosophila Proteins; D006360:Heat-Shock Proteins; D014157:Transcription Factors