title: Use of capillary electrophoresis as a versatile tool to measure interaction constants between a KDR-binding PEGylated lipopeptide and pegylated phospholipid micelles.
abstract: In the frame of our molecular imaging activities, a PEGylated lipopeptide has been developed as a specific ligand for the human vascular endothelial growth factor receptor 2, which is considered as one of the important molecular marker of angiogenesis. In this study, the potential of affinity capillary electrophoresis (ACE) is evaluated to measure the interactions of an active PEGylated lipopeptide, its hydrolysis product and its precursor consisting of a peptide structure with different micelles including Brij-35, Tween-20, and pegylated phospholipids. Given the amphiphilic structure of the PEGylated lipopeptide, a MEKC method allowing the simultaneous separation of the compounds of interest was set up, using low percentages of acetonitrile. Analytes were resolved using a BGE consisting of 100 mM borate buffer pH 9.0, 1 mM Brij, and 25% acetonitrile. Optimized conditions were then used to perform ACE experiments. The affinity constants of the analytes with the micelles were calculated on the basis of their mobility decrease when surfactant concentration increased in the electrolyte. The use of different linearization models to estimate affinity constants was discussed and comparison of different surfactants was reported. PEGylated lipopeptide interacted more strongly with pegylated phospholipid micelles than with Brij-35 or Tween-20. Moreover, it is likely that the chemical structure of the compounds, and particularly the lipidic part of the molecules, significantly affects the interaction with micelles. In conclusion, the ACE method can be readily applied to investigate interactions of our targeting lipopeptides with various micelles currently used for the preparation of pharmaceutical vehicles.
authors.affiliation: Bracco Suisse SA, Plan-les-Ouates, Switzerland.
authors.forename: Martine
authors.initials: M
authors.lastname: Poitevin
authors.forename: François
authors.initials: F
authors.lastname: Tranquart
authors.forename: Samir
authors.initials: S
authors.lastname: Cherkaoui
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D002021:Buffers; D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D016014:Linear Models; D055666:Lipopeptides / Q000032:analysis / Q000378:metabolism*; D008823:Micelles; D057054:Molecular Imaging / Q000379:methods; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011136:Polysorbates / Q000737:chemistry; D011409:Propylene Glycols / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism*
chemical_list: D000097:Acetonitriles; D002021:Buffers; D004573:Electrolytes; D055666:Lipopeptides; D008823:Micelles; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D011108:Polymers; D011136:Polysorbates; D011409:Propylene Glycols; D012997:Solvents; C038089:1,2-distearoylphosphatidylethanolamine; C012504:polypropylene glycol; D011092:Polyethylene Glycols; C000588866:KDR protein, human; D040301:Vascular Endothelial Growth Factor Receptor-2; C515901:Brij 35; C032159:acetonitrile
keywords: ACE; Micelles; Molecular imaging; PEGylated lipopeptide; VEGFR2 receptor