title: Superparamagnetic iron oxide labeling limits the efficacy of rabbit immature dendritic cell vaccination by decreasing their antigen uptake ability in a lysosome-dependent manner.
abstract: Immature dendritic cells (iDCs) are for cell transplantation; however, no method has yet been developed for in vivo monitoring the transplanted iDCs. We have explored the feasibility of using superparamagnetic iron oxide (SPIO) labeling and magnetic resonance imaging for in vivo tracking of transplanted iDCs and determined the effects of SPIO labeling on iDC vaccination. With up to 50 μg Fe/ml, SPIO effectively labeled the iDCs without affecting their growth. At or above 100 μg Fe/ml, SPIO caused considerable damage to iDCs. SPIO labeling resulted in autophagosome formation and decreased the uptake of oxidized low density lipoprotein (ox-LDL), an exogenous antigen, by iDCs. SPIO and ox-LDL both localized to the lysosomes, and this competition for lysosomes could be partially responsible for the decreased ox-LDL phagocytic capacity of iDCs due to SPIO labeling.
authors.affiliation: MOE Key Laboratory of Laser Life Science, South China Normal University, Guangzhou, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.forename: Jingchun
authors.initials: J
authors.lastname: Wang
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhou
authors.forename: Jin
authors.initials: J
authors.lastname: Fang
authors.forename: Chengqian
authors.initials: C
authors.lastname: Zhou
authors.forename: WenLi
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D001241:Aspirin; D001343:Autophagy / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003713:Dendritic Cells / Q000187:drug effects* / Q000378:metabolism; D008247:Lysosomes / Q000187:drug effects* / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D011817:Rabbits; D014611:Vaccination
chemical_list: D058185:Magnetite Nanoparticles; D001241:Aspirin