title: Anti-inflammatory and antinociceptive activity of Urera aurantiaca.
abstract: Urera aurantiaca Wedd. (Urticaceae) is a medicinal plant commonly used in traditional medicine to relieve pain in inflammatory processes. In the present study, the in vivo anti-inflammatory and antinociceptive effects of U. aurantiaca methanolic extract and its possible mechanisms of action were investigated. The extract showed anti-inflammatory activity in the ear edema in mice test (34.3% inhibition), myeloperoxidase (MPO) activity was markedly reduced in animals administered with the extract: within 49.6% and 68.5%. In the histological analysis, intense dermal edema and intense cellular infiltration of inflammatory cells were markedly reduced in the ear tissue of the animals treated with the extract. In the carrageenan-induced hind paw edema in rats assay the extract provoked a significant inhibition of the inflammation (45.5%, 5 h after the treatment) and the MPO activity was markedly reduced (maximum inhibition 71.7%), The extract also exhibited significant and dose-dependent inhibitory effect on the increased vascular permeability induced by acetic acid. The extract presented antioxidant activity in both 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azinobis 3-ethylbenzothiazoline 6-sulfonic acid tests and its total phenol content was 35.4 ± 0.06 mg GAE/g of extract. Also, the extract produced significant inhibition on nociception induced by acetic acid (ED50 : 8.7 mg/kg, i.p.) administered intraperitoneally and orally. Naloxone significantly prevented this activity.
authors.affiliation: Cátedra de Farmacología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, C1113AAD, Buenos Aires, Argentina.
authors.forename: R
authors.initials: R
authors.lastname: Riedel
authors.forename: C
authors.initials: C
authors.lastname: Marrassini
authors.forename: C
authors.initials: C
authors.lastname: Anesini
authors.forename: S
authors.initials: S
authors.lastname: Gorzalczany
mesh_terms: D019342:Acetic Acid / Q000009:adverse effects; D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002351:Carrageenan / Q000009:adverse effects; D004487:Edema / Q000188:drug therapy; D005260:Female; D016166:Free Radical Scavengers / Q000737:chemistry; D007249:Inflammation / Q000188:drug therapy; D008297:Male; D051379:Mice; D010146:Pain / Q000188:drug therapy; D009195:Peroxidase / Q000378:metabolism; D008517:Phytotherapy; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D027003:Urticaceae / Q000737:chemistry*
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D016166:Free Radical Scavengers; D010936:Plant Extracts; D002351:Carrageenan; D009195:Peroxidase; D019342:Acetic Acid
keywords: Urera aurantiaca; anti-inflammatory activity; antinociceptive activity; antioxidant activity; in vivo