title: The isolation and antioxidant activity of polysaccharides from the marine microalgae Isochrysis galbana.
abstract: Three polysaccharides, IPSI-A, IPSI-B and IPSII, were successfully isolated from the marine microalgae Isochrysis galbana through a combination of anion-exchange column chromatography and repeated gel chromatography. These three polysaccharides were demonstrated to have moderate scavenging activities against superoxide and hydroxyl radicals and moderate reductive power in a concentration-dependent manner. The IPSII demonstrated more effective antioxidant activities than IPSI-A and IPSI-B. IPSII had a molecular weight of 15.934 kDa belonging to a β-type heteropolysaccharide with a pyran group and primarily contained mannose with variable amounts of glucose, galactose and rhamnose based on an analysis of infrared spectroscopy (IR), electrospray ionization-mass spectrometry (ESI-MS) and (1)H nuclear magnetic resonance ((1)H NMR).
authors.affiliation: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, PR China. Electronic address: syy-999@163.com.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Beijing Institute of Medical Device Testing, Beijing 101111, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, PR China.
authors.forename: Ganlin
authors.initials: G
authors.lastname: Guo
authors.affiliation: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, PR China.
authors.forename: Yinfang
authors.initials: Y
authors.lastname: Pu
authors.affiliation: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, PR China.
authors.forename: Binlun
authors.initials: B
authors.lastname: Yan
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D002845:Chromatography; D058087:Haptophyta / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D013055:Spectrophotometry, Infrared; D013481:Superoxides / Q000737:chemistry
chemical_list: D000975:Antioxidants; D009005:Monosaccharides; D011134:Polysaccharides; D013481:Superoxides; D017665:Hydroxyl Radical
keywords: Antioxidant activities; Isochrysis galbana; Isolation; Polysaccharides