title: The efficient production of 3β,7α,15α-trihydroxy-5-androsten-17-one from dehydroepiandrosterone by Gibberella intermedia.
abstract: The biotransformation of dehydroepiandrosterone (DHEA) by Gibberella intermedia was investigated. The formation of the main products was monitored by high-performance liquid chromatography (HPLC). Products were purified and identified as 3β,7α-dihydroxy-5-androsten-17-one (7α-OH-DHEA) and 3β,7α,15α-trihydroxy-5-androsten-17-one (7α,15α-diOH-DHEA) using mass spectroscopy (MS) and nuclear magnetic resonance (NMR) analyses. 7α,15α-DiOH-DHEA is a key intermediate for the synthesis of pharmacologically active steroids. A biotransformation process was optimized to obtain a high concentration of 7α,15α-diOH-DHEA. The results showed the optimal biotransformation process under the following conditions: a culture medium containing 15 g/L glucose, 16 g/L yeast extract, 12 g/L corn steep liquor, and 0.15 g/L ferrous sulfate; an initial pH of 6.5; culture temperature of 30 °C; inoculum size of 4 %; 40 mL volume of the culture medium in 250 mL flasks; the addition of 1 % Tween 80 as co-solvent; and the transformation period of 78 h. After optimization, the molar yield of 7α,15α-diOH-DHEA from 5 g/L substrate DHEA was 77.4 %, which was increased by 115 % than the yield obtained in the original bioconversion process.
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, 1800 Lihu Avenue, 214122, Wuxi, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Fu
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Zhang
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.forename: Jinsong
authors.initials: J
authors.lastname: Shi
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Xu
mesh_terms: D001711:Biotransformation / Q000502:physiology; D003687:Dehydroepiandrosterone / Q000737:chemistry / Q000378:metabolism*; D005874:Gibberella / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry
chemical_list: D003687:Dehydroepiandrosterone