title: [MALDI mass spectrometry of the meniscus. Objectification of morphological findings].
abstract: BACKGROUND
  The classification of meniscal lesions requires correlation with clinical data. For the standardization of histopathology reports a discrimination between normal, low-grade lesions and high-grade lesions is feasible. This classification can be further specified using other methods.
  
  
  METHODS
  Formalin-fixed, paraffin-embedded specimens of meniscal tissue from 68 patients were analyzed by matrix-assisted laser desorption ionization (MALDI) imaging.
  
  
  RESULTS
  The classification of meniscal lesions and differentiation between low-grade and high-grade and acute versus non-acute degeneration is possible by determination of the differential expression of mass-to-charge ratios by statistical comparisons using the P-value from combined Wilcoxon and Kruskal-Wallis (PWKW) tests and a predefined average two-fold difference in intensity.
  
  
  CONCLUSIONS
  The concept of a "meniscus report" is introduced for documentation of meniscus tissue specimens integrating histological, histochemical and proteomic data, thereby specifying the degree of degeneration and the assessment of acute or non-acute lesions. Mass spectrometry contributes to an objective histopathology report. An advisory opinion should always be based on close correlation of clinical and morphological evaluations.
authors.affiliation: Zentrum für Molekulare Orthopädie, Gemeinschaftspraxis Königsallee, Düsseldorf, Deutschland.
authors.forename: J
authors.initials: J
authors.lastname: Petzold
authors.forename: R
authors.initials: R
authors.lastname: Casadonte
authors.forename: M
authors.initials: M
authors.lastname: Otto
authors.forename: M
authors.initials: M
authors.lastname: Kriegsmann
authors.forename: M
authors.initials: M
authors.lastname: Granrath
authors.forename: A
authors.initials: A
authors.lastname: Baltzer
authors.forename: J
authors.initials: J
authors.lastname: Vogel
authors.forename: P
authors.initials: P
authors.lastname: Drees
authors.forename: S
authors.initials: S
authors.lastname: Deininger
authors.forename: M
authors.initials: M
authors.lastname: Becker
authors.forename: J
authors.initials: J
authors.lastname: Kriegsmann
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000465:Algorithms; D015415:Biomarkers / Q000032:analysis; D003936:Diagnosis, Computer-Assisted / Q000379:methods*; D005260:Female; D050724:Fractures, Cartilage / Q000175:diagnosis*; D006801:Humans; D008297:Male; D008592:Menisci, Tibial / Q000737:chemistry* / Q000473:pathology; D008875:Middle Aged; D010363:Pattern Recognition, Automated / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D060388:Support Vector Machine; D055815:Young Adult
chemical_list: D015415:Biomarkers