title: Mouse intestinal innate immune responses altered by enterotoxigenic Escherichia coli (ETEC) infection.
abstract: Enterotoxigenic Escherichia coli (ETEC) is an important cause of human and porcine morbidity and mortality. The current study was conducted to identify intestinal immunity that is altered in a mouse model of ETEC infection. Innate immune responses and inflammation were analyzed. The activation of signal transduction pathways, including toll like receptor 4 (TLR-4)-nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPK), was analyzed using immunoblotting and PCR array analyses. We found that ETEC infection promoted the expression of pro-inflammatory cytokines through the activation of the NF-κB and MAPK pathways. Meanwhile, ETEC infection affected sIgA transportation and Paneth cell function. These data improve our understanding of how ETEC causes disease in animals.
authors.affiliation: Laboratory of Animal Nutrition and Health and Key Laboratory of Agro-Ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, PR China.
authors.forename: Wenkai
authors.initials: W
authors.lastname: Ren
authors.affiliation: Laboratory of Animal Nutrition and Health and Key Laboratory of Agro-Ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yin
authors.affiliation: Laboratory of Animal Nutrition and Health and Key Laboratory of Agro-Ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, PR China.
authors.forename: Jielin
authors.initials: J
authors.lastname: Duan
authors.affiliation: Laboratory of Animal Nutrition and Health and Key Laboratory of Agro-Ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, PR China. Electronic address: gangle.liu@gmail.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Division of Immunology, Virginia-Maryland Regional College of Veterinary Medicine, Maryland Pathogen Research Institute, University of Maryland, College Park, MD 20742, USA.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Laboratory of Animal Nutrition and Health and Key Laboratory of Agro-Ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Chen
authors.affiliation: Laboratory of Animal Nutrition and Health and Key Laboratory of Agro-Ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, PR China.
authors.forename: Tiejun
authors.initials: T
authors.lastname: Li
authors.affiliation: Laboratory of Animal Nutrition and Health and Key Laboratory of Agro-Ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, PR China.
authors.forename: Shengping
authors.initials: S
authors.lastname: Wang
authors.affiliation: Laboratory of Animal Nutrition and Health and Key Laboratory of Agro-Ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, PR China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS 66506, USA.
authors.forename: Philip R
authors.initials: PR
authors.lastname: Hardwidge
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D003967:Diarrhea / Q000276:immunology* / Q000382:microbiology*; D004195:Disease Models, Animal; D054307:Enterotoxigenic Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology* / Q000382:microbiology*; D020869:Gene Expression Profiling; D006801:Humans; D007113:Immunity, Innate; D015151:Immunoblotting; D007071:Immunoglobulin A, Secretory / Q000378:metabolism; D051379:Mice; D019879:Paneth Cells / Q000502:physiology; D016133:Polymerase Chain Reaction; D021381:Protein Transport; D015398:Signal Transduction
chemical_list: D016207:Cytokines; D007071:Immunoglobulin A, Secretory
keywords: ETEC; Innate immunity; MAPK; NF-κB; Paneth cell