title: Differential expression of miR-155 and miR-21 in tumor and stroma cells in diffuse large B-cell lymphoma.
abstract: OncomiRs miR-21 and miR-155 have been linked to lymphomagenesis, but information on their implication in diffuse large B-cell lymphoma (DLBCL) is limited. Here, we used locked nucleic acid-based in situ hybridization (ISH) detection techniques on formalin-fixed paraffin-embedded DLBCL tissue samples to identify miR-155 and miR-21 at the cellular level in 56 patients diagnosed with DLBCL, and compared them to miR array data. miR-155 was observed in tumor cells in 19/56 (33.9%) of the samples evaluated by ISH. miR-21 was localized to the stromal compartment in 41/56 (73.2%). A subset of these, 16/56 (28.6%), also showed labeling in tumor cells. When comparing ISH-scores and miR array data, miR-155 in tumor cells, identified by ISH, was associated with miR-155 expression in miR array data (P=0.030). Equally, miR-21 expression by miR array data were highly associated with miR-21 ISH-scores in the stromal cells (P=0.002), whereas no association between miR array data and ISH of miR-21 in tumor cells was observed (P=0.673). We found no association of miR-155 and miR-21 with overall survival or germinal center B-cell-like (GCB) versus non-GCB-like subtypes of DLBCL. In conclusion, miR-ISH added to the biological interpretation of miR expression in DLBCL compared with miR array data, but miR-155 and miR-21 ISH did not add prognostic information in this series.
authors.affiliation: Departments of *Pathology †Hematology, Copenhagen University Hospital, Copenhagen ‡Exiqon A/S, Diagnostic Product Development, Vedbæk, Denmark.
authors.forename: Helga D
authors.initials: HD
authors.lastname: Munch-Petersen
authors.forename: Ulrik
authors.initials: U
authors.lastname: Ralfkiaer
authors.forename: Lene D
authors.initials: LD
authors.lastname: Sjö
authors.forename: Christoffer
authors.initials: C
authors.lastname: Hother
authors.forename: Fazila
authors.initials: F
authors.lastname: Asmar
authors.forename: Boye S
authors.initials: BS
authors.lastname: Nielsen
authors.forename: Peter
authors.initials: P
authors.lastname: Brown
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Ralfkiaer
authors.forename: Kirsten
authors.initials: K
authors.lastname: Grønbæk
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000378:metabolism* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D008875:Middle Aged; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology
chemical_list: C524942:MIRN155 microRNA, human; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D012334:RNA, Neoplasm