title: Purification, characterization and antitumor activity of polysaccharides from Pleurotus eryngii residue.
abstract: A novel water-soluble polysaccharide from Pleurotus eryngii residue was isolated and further purified by DEAE cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography to yield PEPE-1, PEPE-2 and PEPE-3. Molecular weights were determined by high-performance size-exclusion chromatography (HPSEC). Gas chromatography (GC) analysis of monosaccharide composition confirmed that PEPE-1, PEPE-2 and PEPE-3 were heteropolysaccharides and mainly composed of glucose. Sulfate and uronic acid content, ultraviolet and infrared spectrum were also evaluated. The antitumor activities of the polysaccharides against HepG-2 cells were studied in vitro. Results showed that the three polysaccharides could suppress the proliferation and enhance lactate dehydrogenase (LDH) release of HepG-2 cells in a dose- and time-dependent manner. The effect increased in the order of PEPE-1<PEPE-2<PEPE-3, respectively. The same order was also observed with uronic acid content. Findings presented in this study suggested that the polysaccharides extracted from P. eryngii residue might be suitable for functional foods and natural antitumor drugs development.
authors.affiliation: College of Food Science and Engineering, Nanjing University of Finance and Economics, No. 3 Wenyuan Road, Nanjing 210023, PR China.
authors.forename: Gaoxing
authors.initials: G
authors.lastname: Ma
authors.affiliation: College of Food Science and Engineering, Nanjing University of Finance and Economics, No. 3 Wenyuan Road, Nanjing 210023, PR China.
authors.forename: Wenjian
authors.initials: W
authors.lastname: Yang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, PR China; Department of Dairy and Food Science and Technology, Egerton University, Egerton 536, Nakuru, Kenya.
authors.forename: Alfred Mugambi
authors.initials: AM
authors.lastname: Mariga
authors.affiliation: College of Food Science and Engineering, Nanjing University of Finance and Economics, No. 3 Wenyuan Road, Nanjing 210023, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Fang
authors.affiliation: College of Food Science and Engineering, Nanjing University of Finance and Economics, No. 3 Wenyuan Road, Nanjing 210023, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Pei
authors.affiliation: College of Food Science and Engineering, Nanjing University of Finance and Economics, No. 3 Wenyuan Road, Nanjing 210023, PR China; College of Food Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, PR China. Electronic address: qiuhuihu@njue.edu.cn.
authors.forename: Qiuhui
authors.initials: Q
authors.lastname: Hu
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002849:Chromatography, Gas; D056945:Hep G2 Cells; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; D007770:L-Lactate Dehydrogenase
keywords: Anti-proliferation activity; Characterization; Isolation; Pleurotus eryngii residue; Polysaccharide