title: Effects of medium components and culture conditions on mycelial biomass and the production of bioactive ingredients in submerged culture of Xylaria nigripes (Ascomycetes), a Chinese medicinal fungus.
abstract: The optimal culture conditions were investigated to maximize the production of mycelial biomass and bioactive ingredients in submerged cultivation of Xylaria nigripes, a Chinese medicinal fungus. The one-factor-at-a-time method was used to explore the effects of medium components, including carbon, nitrogen, mineral sources, and initial pH of the medium and environmental factors, such as culture temperature and rotation speed, on mycelial growth and production of bioactive ingredients. The results indicated that the optimal culture temperature and rotation speed were 25°C and 100 rpm in a medium with 20 g fructose, 6 g yeast extract, and 2 g magnesiun sulfate heptahydrate as carbon, nitrogen, and mineral sources, respectively, in 1 L distilled water with an initial medium pH of 5.5. With optimal medium components and conditions of cultivation, the maximal production of mycelial biomass was 6.64 ± 0.88 g/L, with maximal production of bioactive ingredients such as extracellular polysaccharides (2.36 ± 0.18 mg/mL), intracellular polysaccharides (2.38 ± 0.07 mg/g), adenosine (43.27 ± 2.37 mg/g), total polyphenols (36.57 ± 1.36 mg/g), and triterpenoids (31.29 ± 1.17 mg/g) in a shake flask culture. These results suggest that different bioactive ingredients including intracellular polysaccharides, adenosine, total polyphenols and triterpenoids in mycelia and extracellular polysaccharides in broth can be obtained from one simple medium for submerged cultivation of X. nigripes.
authors.affiliation: Department of Bioindustry Technology, Da-Yeh University, Datsuen, Changhua, Taiwan.
authors.forename: Jian-Zhi
authors.initials: JZ
authors.lastname: Chen
authors.affiliation: Department of Nutritional Science, Fu Jen Catholic University, New Taipei City, Taiwan.
authors.forename: Hui-Chen
authors.initials: HC
authors.lastname: Lo
authors.affiliation: Department of Bioindustry Technology, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan.
authors.forename: Fang-Yi
authors.initials: FY
authors.lastname: Lin
authors.affiliation: School of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Changhua, Taiwan.
authors.forename: Shih-Liang
authors.initials: SL
authors.lastname: Chang
authors.affiliation: Department of Medicinal Botanicals and Healthcare, Da-Yeh University, Datsuen, Changhua, Taiwan.
authors.forename: Changwei
authors.initials: C
authors.lastname: Hsieh
authors.affiliation: Department of Bioresources, Da-Yeh University, Datsuen, Changhua 51591, Taiwan.
authors.forename: Zeng-Chin
authors.initials: ZC
authors.lastname: Liang
authors.affiliation: Department of Medicinal Botanicals and Healthcare, Department of Bioresources, Da-Yeh University, Datsun, Changhua, 51591 Taiwan.
authors.forename: Wai-Jane
authors.initials: WJ
authors.lastname: Ho
authors.affiliation: Department of Bioindustry Technology, Department of Medicinal Botanicals and Healthcare, Da-Yeh University, Datsuen, Changhua, Taiwan.
authors.forename: Tai-Hao
authors.initials: TH
authors.lastname: Hsu
mesh_terms: D001688:Biological Products / Q000378:metabolism*; D018533:Biomass; D003470:Culture Media / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D025282:Mycelium / Q000254:growth & development* / Q000378:metabolism*; D013696:Temperature; D013098:Xylariales / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D001688:Biological Products; D003470:Culture Media