title: A liquid chromatography-tandem mass spectrometry assay for quantification of rilpivirine and dolutegravir in human plasma.
abstract: A liquid chromatography-tandem mass spectrometry assay requiring a 100μL aliquot of human plasma for simultaneous determination of rilpivirine, a second generation non-nucleoside reverse transcriptase inhibitors of HIV and dolutegravir, a novel integrase stand transfer inhibitors of HIV concentrations has been developed. Sample pre-treatment is limited to protein precipitation with a mixture of methanol and zinc sulfate. After centrifugation the supernatant is injected in the chromatographic system, which consists of on-line solid phase extraction followed by separation on a phenyl-hexyl column. This 2.5min method, with its simple sample preparation provides sensitive (the limit of quantitation is 25ng/mL for each compound), accurate and precise (the intra-day and inter-day imprecision and inaccuracy are lower than 15%) quantification of the plasma concentration of these drugs and can be used for therapeutic drug monitoring in patients infected with HIV.
authors.affiliation: Clinical Pharmacology Department, CHU de Nantes, Nantes, France.
authors.forename: M
authors.initials: M
authors.lastname: Grégoire
authors.affiliation: Clinical Pharmacology Department, CHU de Nantes, Nantes, France.
authors.forename: G
authors.initials: G
authors.lastname: Deslandes
authors.affiliation: Clinical Pharmacology Department, CHU de Nantes, Nantes, France.
authors.forename: C
authors.initials: C
authors.lastname: Renaud
authors.affiliation: Clinical Pharmacology Department, CHU de Nantes, Nantes, France; EA 4275 Biostatistique, Recherche Clinique et Mesures Subjectives en Santé, Faculté de Médecine-Pharmacie, Université de Nantes, France.
authors.forename: R
authors.initials: R
authors.lastname: Bouquié
authors.affiliation: Infectious Diseases Department, CHU de Nantes, Nantes, France.
authors.forename: C
authors.initials: C
authors.lastname: Allavena
authors.affiliation: Infectious Diseases Department, CHU de Nantes, Nantes, France.
authors.forename: F
authors.initials: F
authors.lastname: Raffi
authors.affiliation: Clinical Pharmacology Department, CHU de Nantes, Nantes, France; EA 4275 Biostatistique, Recherche Clinique et Mesures Subjectives en Santé, Faculté de Médecine-Pharmacie, Université de Nantes, France.
authors.forename: P
authors.initials: P
authors.lastname: Jolliet
authors.affiliation: Clinical Pharmacology Department, CHU de Nantes, Nantes, France; EA 3826 Thérapeutiques Cliniques et Expérimentales des Infections, Faculté de Médecine-Pharmacie, Université de Nantes, France. Electronic address: eric.dailly@chu-nantes.fr.
authors.forename: E
authors.initials: E
authors.lastname: Dailly
mesh_terms: D019380:Anti-HIV Agents / Q000097:blood*; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods; D015658:HIV Infections / Q000097:blood / Q000188:drug therapy; D019428:HIV Integrase Inhibitors; D006575:Heterocyclic Compounds, 3-Ring / Q000097:blood*; D006801:Humans; D009570:Nitriles / Q000097:blood*; D010078:Oxazines; D010879:Piperazines; D011728:Pyridones; D011743:Pyrimidines / Q000097:blood*; D018894:Reverse Transcriptase Inhibitors; D000068696:Rilpivirine; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D000099226:Dolutegravir
chemical_list: D019380:Anti-HIV Agents; D019428:HIV Integrase Inhibitors; D006575:Heterocyclic Compounds, 3-Ring; D009570:Nitriles; D010078:Oxazines; D010879:Piperazines; D011728:Pyridones; D011743:Pyrimidines; D018894:Reverse Transcriptase Inhibitors; D000068696:Rilpivirine; D000099226:Dolutegravir
keywords: Dolutegravir; Mass spectrometry; Rilpivirine