title: Genomic organization and promoter analysis of a transcriptional repressor gene from Fenneropenaeus chinensis.
abstract: In this study, we cloned and sequenced genomic sequences from a Fenneropenaeus chinensis transcriptional repressor gene, FcTR. The FcTR gene is 2,671 bp in length and has four exons and three introns. The 873 bp promoter contains several transcription factor binding sites, including a TATA box and a cyclic AMP-responsive element. Promoter deletion analysis using a luciferase reporter gene identified regulatory elements. Challenge with white spot syndrome virus increased expression from the promoter-deletion constructs. These results suggest that FcTR might play an important role in the shrimp immune response.
authors.affiliation: Ocean and Fisheries College, Huaihai Institute of Technology, Lianyungang, 222005, China, lai.xiaofang@163.com.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Lai
authors.forename: Shanrui
authors.initials: S
authors.lastname: Shen
authors.forename: Huan
authors.initials: H
authors.lastname: Gao
authors.forename: Binlun
authors.initials: B
authors.lastname: Yan
mesh_terms: D000818:Animals; D001483:Base Sequence; D003445:Crustacea / Q000235:genetics*; D015870:Gene Expression; D016678:Genome; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D012097:Repressor Proteins