title: Speciation of chromium in chromium yeast.
abstract: High-performance liquid chromatography was used to separate Cr(III) and Cr(VI) in samples with detection by inductively coupled plasma mass spectrometry(ICP-MS). The separation was achieved on a weak anion exchange column. The mobile phase was pH 7.0 ammonium nitrate solution. The redox reaction between Cr(III) and Cr(VI) was avoided during separation and determination. This separation method could be used to separate the samples with large concentration differences between Cr(III) and Cr(VI). The alkaline digestion was used to extract chromium in solid sample, which had no effect on the retention time and the peak area of the Cr(VI). However, the conversion of Cr(VI) from Cr(III) was observed during alkaline digestion, which displayed positive relation with the ratio of Cr(III) and Cr(VI) in samples. Both Cr(III) and Cr(VI) contents of chromium yeasts cultured in media with different chromium additions were determined. The spike recoveries of Cr(VI) for chromium yeasts were in the range of 95-108 %.
authors.affiliation: CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China.
authors.forename: Xuena
authors.initials: X
authors.lastname: Guo
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Xuejing
authors.initials: X
authors.lastname: Bai
authors.forename: Xiuping
authors.initials: X
authors.lastname: He
authors.forename: Borun
authors.initials: B
authors.lastname: Zhang
mesh_terms: D002857:Chromium / Q000032:analysis*; D003470:Culture Media / Q000737:chemistry; D007477:Ions / Q000032:analysis*; D013058:Mass Spectrometry; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000254:growth & development
chemical_list: D003470:Culture Media; D007477:Ions; D002857:Chromium