title: [Th17/Treg unbalance is involved in the pathogenesis of experimental autoimmune encephalomyelitis].
abstract: OBJECTIVE
  To investigate the role of Th17/Treg unbalance in the pathogenesis of experimental autoimmune encephalomyelitis (EAE).
  
  
  METHODS
  EAE was modeled in mice and the number of regulatory T cells (Tregs) in spleen of EAE mice was detected by flow cytometry. The expressions of Foxp3 and RoR-γt mRNA in the spleen of EAE mice and IL-17 mRNA in the brain of EAE mice were evaluated by real-time quantitative PCR and the levels of IL-6, TGF-β and IL-17 in the serum of EAE mice were examined by ELISA.
  
  
  RESULTS
  Compared with control group, the number of CD4(+)CD25(+) Foxp3(+) Tregs and the expression of Foxp3 mRNA in the spleen of EAE mice dramatically decreased in the early and peak stage of EAE (P<0.05), but increased in chronic stage of EAE (P<0.05); the RoR-γt mRNA expression from mouse spleen at the early stage of EAE was significant raised (P<0.05), but was not significantly different at the peak and chronic stage of EAE from that in control group (P>0.05). The levels of IL-6 and TGF-β in the serum of EAE group dramatically increased compared with control group (P<0.05). With the development of EAE, the level of IL-6 gradually decreased, and there was no statistical difference in the chronic stage of EAE compared with control group (P>0.05). However, the level of TGF-β was higher than that in control group in the chronic stage of EAE (P<0.05). Compared with those in control group, the concentration of IL-17A and the expression of IL-17 mRNA dramatically increased in different stages of EAE group, especially in peak stage (P<0.05).
  
  
  CONCLUSIONS
  Th17/Treg unbalance may be involved in the pathogenesis of EAE.
authors.affiliation: Department of Clinical Laboratory, Union Hospital, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Pingxia
authors.initials: P
authors.lastname: Lu
authors.affiliation: Department of Clinical Laboratory, Union Hospital, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Meihua
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Union Hospital, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Peizheng
authors.initials: P
authors.lastname: Zheng
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Xiamen University, Xiamen 361022, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Hou
authors.affiliation: Department of Clinical Laboratory, Union Hospital, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Union Hospital, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Clinical Laboratory, Union Hospital, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Yingping
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000097:blood / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000276:immunology; D015870:Gene Expression / Q000276:immunology; D020381:Interleukin-17 / Q000097:blood / Q000235:genetics / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000097:blood / Q000235:genetics / Q000276:immunology; D018655:Lymphocyte Count; D008810:Mice, Inbred C57BL; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D058504:Th17 Cells / Q000276:immunology* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000097:blood / Q000235:genetics / Q000276:immunology
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D020381:Interleukin-17; D053645:Interleukin-2 Receptor alpha Subunit; D015850:Interleukin-6; D016212:Transforming Growth Factor beta