title: Cartilage Regeneration of Adipose-Derived Stem Cells in the TGF-β1-Immobilized PLGA-Gelatin Scaffold.
abstract: Articular cartilage has restricted self-regenerative capacity; therefore, treatment of cartilage lesions is a great challenge in the field of orthopedics. In the present study, we evaluate the enhancing effect of a transforming growth factor-beta 1 (TGF-β1)-immobilized scaffold, fabricated by incorporating TGF-β1-loaded gelatin microspheres into PLGA framework, on the differentiation of adipose-derived stem cells (ASCs) into chondrocytes. Significant increase in cell proliferation was observed in the TGF-β1-immobilized PLGA-gelatin scaffold, as compared with the ASC-seeded non-TGF-β1-immobilized PLGA-gelatin scaffold. When chondrogenic differentiation of ASCs was evaluated for both constructs, sulfated glycosaminoglycan (sGAG) content was significantly higher in the TGF-β1-immobilized scaffold. This study showed that ASCs containing the TGF-β1-immobilized scaffold better promoted cartilage regeneration in defective articular cartilage, which is assessed by histological observation. Based on the above results, we conclude that TGF-β1-immobilized PLGA-gelatin scaffold seeded with ASCs considerably enhances the quality of the tissue-engineered cartilage, therefore, advancing the field of cartilage tissue engineering.
authors.affiliation: Department of Joint and Bone Disease Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yin
authors.forename: Junfeng
authors.initials: J
authors.lastname: Cai
authors.forename: Wen
authors.initials: W
authors.lastname: Zen
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Wei
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Feng
authors.initials: F
authors.lastname: Yuan
authors.forename: Shree Ram
authors.initials: SR
authors.lastname: Singh
authors.forename: Yiyong
authors.initials: Y
authors.lastname: Wei
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects; D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D005780:Gelatin / Q000008:administration & dosage / Q000737:chemistry; D019344:Lactic Acid / Q000008:administration & dosage / Q000737:chemistry; D011100:Polyglycolic Acid / Q000008:administration & dosage / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D012038:Regeneration / Q000187:drug effects*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000009:adverse effects; D016212:Transforming Growth Factor beta / Q000008:administration & dosage / Q000737:chemistry*
chemical_list: D016212:Transforming Growth Factor beta; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005780:Gelatin