title: Hydrogel-based ultra-moisturizing cream formulation for skin hydration and enhanced dermal drug delivery.
abstract: To develop an external vehicle for skin hydration and enhanced dermal drug delivery, a hydrogel-based ultra-moisturizing cream (HUMC) was successfully formulated with carbopol 934P, urea, Tinocare GL, grape seed oil, and other excipients. The HUMC showed plastic flow behavior due to a gel structure with a cream base. Different types of drug-free vehicles such as a hydrogel, conventional cream (CC), and three HUMCs were prepared and subjected to an in vivo skin hydration test on a hairless mouse using a corneometer. Hydration effect (∆AU) was in the order of HUMC2>HUMC1 ≥ CC>HUMC3>hydrogel. Using nile red (NR) and 5-carboxyfluorescein (5-CF) as lipophilic and hydrophilic fluorescent probes, respectively, in vitro skin permeation and accumulation studies were conducted using Franz diffusion cells. The values of steady-state flux (Jss, ng/h/cm(2)) were obtained: 74.8 (CC), 145.6 (HUMC1), and 161.9 (HUMC2) for NR delivery; 6.8 (CC), 8.3 (HUMC1), and 10.9 (HUMC2) for 5-CF delivery. The amounts retained in the skin at 12 h (Qr, ng/cm(2)) were determined: 86.4 (CC) and 102.0 (HUMC2) for NR; and 70.1 (CC) and 195.6 (HUMC2) for 5-CF. Confocal microscopy was used to visualize the distribution of the fluorescent probes. NR tended to be localized into the deeper part of the skin with adipose tissue whereas 5-CF localized in the upper layer of the skin. Thus we propose that HUMC2 is an efficacious vehicle for skin hydration and enhances dermal delivery of lipophilic and hydrophilic drugs.
authors.affiliation: College of Pharmacy, Chung-Ang University.
authors.forename: Sang Gon
authors.initials: SG
authors.lastname: Lee
authors.forename: Sung Rae
authors.initials: SR
authors.lastname: Kim
authors.forename: Hye In
authors.initials: HI
authors.lastname: Cho
authors.forename: Mean Hyung
authors.initials: MH
authors.lastname: Kang
authors.forename: Dong Woo
authors.initials: DW
authors.lastname: Yeom
authors.forename: Seo Hyun
authors.initials: SH
authors.lastname: Lee
authors.forename: Sangkil
authors.initials: S
authors.lastname: Lee
authors.forename: Young Wook
authors.initials: YW
authors.lastname: Choi
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D016503:Drug Delivery Systems / Q000379:methods*; D004643:Emollients / Q000008:administration & dosage* / Q000378:metabolism; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000008:administration & dosage* / Q000378:metabolism; D055103:Hypodermoclysis / Q000379:methods*; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D009924:Organ Culture Techniques; D012869:Skin Absorption / Q000187:drug effects* / Q000502:physiology; D063465:Skin Cream / Q000008:administration & dosage* / Q000378:metabolism
chemical_list: D004643:Emollients; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate