title: [Effects of activation of ALDH2 by ethanol on the expression of JNK in kidney of diabetic rats].
abstract: OBJECTIVE
  To observe the effect of activation of aldehyde dehydrogenase 2 (ALDH2) by ethanol on the expression of c-Jun N-terminal kinase (JNK) in the kidney of diabetic rats.
  
  
  METHODS
  Eightheen healthy male SD rats were randomly divided into 3 groups (n = 6): normal control group, diabetes group and ethanol + diabetes group. After 8 weeks, 24 h urine samples from rats were collected to detect urinary protein content. The kidney was isolated and the ratio of kidney weight/body weight (index of kidney weight) was detected. The levels of fasting blood glucose, glycosylated hemoglobin serum urea nitrogen and serum creatinine were measured. Morphological changes of renal tissue were observed by optical microscope. The protein expressions of ALDH2 and JNK in renal tissue were detected by Western blot.
  
  
  RESULTS
  Compared with the normal control rats, the levels of fasting blood glucose, glycosylated hemoglobin, serum urea nitrogen, serum creatinine and the index of kidney weight were increased markedly in diabetic rats. The expression of ALDH2 protein was decreased, while p-JNK, JNK protein expressions and the ratio of p-JNK/JNK were increased. The morphological observation was shown that the amount of glomerular mesangial matrix were increased, basement membrane were thickened and capillary lumen were narrowed. However,in ethanol + diabetes group, renal function was improved and the damage of renal structure was attenuated. The expression of ALDH2 protein was increased, while p-JNK, JNK and the ratio of p-JNK/JNK were decreased.
  
  
  CONCLUSIONS
  Enhanced ALDH2 expression can protect kidney in diabetic rats, which may be relevant with inhibitting the activity of JNK pathway.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yu
authors.forename: Pin-Fang
authors.initials: PF
authors.lastname: Kang
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Li
authors.forename: Guan-Jun
authors.initials: GJ
authors.lastname: Zhang
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Wang
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Ye
authors.forename: Qin
authors.initials: Q
authors.lastname: Gao
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000378:metabolism / Q000502:physiology*; D000071396:Aldehyde Dehydrogenase, Mitochondrial; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D000431:Ethanol / Q000494:pharmacology*; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D008297:Male; D024101:Mitochondrial Proteins / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D024101:Mitochondrial Proteins; D000431:Ethanol; D000444:Aldehyde Dehydrogenase; D000071396:Aldehyde Dehydrogenase, Mitochondrial; C505112:Aldh2 protein, rat; D048031:JNK Mitogen-Activated Protein Kinases