title: Creating sub-50 nm nanofluidic junctions in a PDMS microchip via self-assembly process of colloidal silica beads for electrokinetic concentration of biomolecules.
abstract: In this work we describe a novel and simple self-assembly process of colloidal silica beads to create a nanofluidic junction between two microchannels. The nanoporous membrane was used to induce ion concentration polarization inside the microchannel and this electrokinetic preconcentration system allowed rapid concentration of DNA samples by ~1700 times and of protein samples by ~100 times within 5 minutes.
authors.affiliation: New York University Abu Dhabi, Division of Engineering, PO Box 129188, Abu Dhabi, UAE. rafael.song@nyu.edu.
authors.forename: A
authors.initials: A
authors.lastname: Syed
authors.forename: L
authors.initials: L
authors.lastname: Mangano
authors.forename: P
authors.initials: P
authors.lastname: Mao
authors.forename: J
authors.initials: J
authors.lastname: Han
authors.forename: Y-A
authors.initials: YA
authors.lastname: Song
mesh_terms: D003102:Colloids / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D049329:Nanostructures / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D003102:Colloids; D004129:Dimethylpolysiloxanes; D009757:Nylons; D011506:Proteins; C085707:poly(dimethylsiloxane)-polyamide copolymer; D012822:Silicon Dioxide; D004247:DNA