title: [Novel immobilization of arginase I via cellulose-binding domain and its application in producing of L-ornitine].
abstract: The recombinant Escherichia coli strain pET35b-ARG, which overexpresses arginase I fused to a cellulose-binding domain (CBD), was developed. After preparing cellulose microspheres, arginase I was immobilized via the CBD of the fusion protein. Under optimal reaction conditions (40 degrees C, pH 9.5, 1 mM of Mn2+, 30 microl/ml of immobilized enzyme, 30 g/l of L-Arg, and for I h), the conversion rate of L-Arg was 98.7%. After 7 reuses of 30 microl of immobilized enzyme in 1 ml of catalytic solution, 153 mg of L-Orn with 97.3% purity was obtained. This indicated that the immobilization method was effective, feasible and could be used for the industrial production of L-Orn in the future.
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Iang
authors.forename: H
authors.initials: H
authors.lastname: Qu
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: F
authors.initials: F
authors.lastname: Bai
authors.forename: G
authors.initials: G
authors.lastname: Bai
mesh_terms: D001119:Arginase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D001120:Arginine / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002482:Cellulose / Q000737:chemistry; D003001:Cloning, Molecular; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D017743:Equipment Reuse; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D007700:Kinetics; D009952:Ornithine / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D011993:Recombinant Fusion Proteins; D002482:Cellulose; D001120:Arginine; D009952:Ornithine; D001119:Arginase