title: [The establishment of a magnetic nanoparticle-based immunocapturing method for the detection of abrin].
abstract: OBJECTIVE
  To develop a high sensitive and specific method for the detection of abrin.
  
  
  METHODS
  The abrin monoclonal antibody (mAb) 7D1 coated with Fe3O4 magnetic nanoparticles (MNPs) and abrin mAb labeled with horseradish peroxidase (HRP-mAb) were used to establish the immunocapturing method for abrin detection. The results were compared with the traditional double antibody sandwich ELISA.
  
  
  RESULTS
  The detecting linear of immunocapture for abrin was 2.5-60 ng/mL, and the linear regression equation was y=0.012x-0.015 with the detection limit of 2.5 ng/mL. Ricin at different concentrations did not interfere the abrin detection results, which demonstrated that the method had a good specificity . This approach showed good reproducibility with relative standard deviation ranging from 5.18%-8.67%. It could be used for analyzing abrin-contaminated specimens such as water, beverage, and milk, etc. The results of comparison with the conventional double antibody sandwich ELISA indicated that the immunocapture have a broader linear scale, higher sensitivity, and a shorter detection time.
  
  
  CONCLUSIONS
  The developed immunocapturing method can be used for detecting traces of abrin.
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Research Institute of Chemical Defence, Beijing 102205, China.
authors.forename: Longhui
authors.initials: L
authors.lastname: Liang
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Research Institute of Chemical Defence, Beijing 102205, China.
authors.forename: Shilei
authors.initials: S
authors.lastname: Liu
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Research Institute of Chemical Defence, Beijing 102205, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Gao
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Research Institute of Chemical Defence, Beijing 102205, China.
authors.forename: Shikun
authors.initials: S
authors.lastname: Zhou
mesh_terms: D000036:Abrin / Q000032:analysis* / Q000276:immunology; D029912:Abrus / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007158:Immunologic Techniques / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D058185:Magnetite Nanoparticles; D000036:Abrin