title: A versatile fluorescence turn-on assay for highly sensitive detection of tyrosine phosphatase activity.
abstract: A simple but robust fluorescence turn-on assay is developed for highly sensitive detection of PTP1B activity by using calcein as the signaling element.
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, Shaanxi Province, P. R. China. liuch@snnu.edu.cn lzpbd@snnu.edu.cn.
authors.forename: Chenghui
authors.initials: C
authors.lastname: Liu
authors.forename: Fangfang
authors.initials: F
authors.lastname: Wang
authors.forename: Yucong
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhengping
authors.initials: Z
authors.lastname: Li
mesh_terms: D004789:Enzyme Activation; D057075:Enzyme Assays / Q000379:methods*; D005452:Fluoresceins / Q000737:chemistry / Q000378:metabolism; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000032:analysis / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; C516607:PTPN1 protein, human; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; C007740:fluorexon