title: Jumonji demethylases moderate precocious flowering at elevated temperature via regulation of FLC in Arabidopsis.
abstract: As sessile organisms, plants have evolved multiple mechanisms to respond to environmental changes to improve survival. Arabidopsis plants show accelerated flowering at increased temperatures. Here we show that Jumonji-C domain-containing protein JMJ30 directly binds to the flowering-repressor FLOWERING LOCUS C (FLC) locus and removes the repressive histone modification H3 lysine 27 trimethylation (H3K27me3). At elevated temperatures, the JMJ30 RNA and protein are stabilized, and FLC expression is maintained at high levels to prevent extreme precocious flowering. The double mutant of JMJ30 and its homologue JMJ32, grown at elevated temperatures, exhibits an early-flowering phenotype similar to the flc mutant, which is associated with increased H3K27me3 levels at the FLC locus and decreased FLC expression. Furthermore, ectopic expression of JMJ30 causes an FLC-dependent late-flowering phenotype. Taken together, JMJ30/JMJ32-mediated histone demethylation at the FLC locus constitutes a balancing mechanism in flowering control at warm temperatures to prevent premature early flowering.
authors.affiliation: 1] Temasek Life Sciences Laboratory (TLL), 1 Research Link, National University of Singapore, Singapore 117604, Singapore [2] Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Science Drive 4, Singapore 117543, Singapore.
authors.forename: Eng-Seng
authors.initials: ES
authors.lastname: Gan
authors.affiliation: Temasek Life Sciences Laboratory (TLL), 1 Research Link, National University of Singapore, Singapore 117604, Singapore.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Temasek Life Sciences Laboratory (TLL), 1 Research Link, National University of Singapore, Singapore 117604, Singapore.
authors.forename: Jie-Yun
authors.initials: JY
authors.lastname: Wong
authors.affiliation: 1] Temasek Life Sciences Laboratory (TLL), 1 Research Link, National University of Singapore, Singapore 117604, Singapore [2].
authors.forename: Jessamine Geraldine
authors.initials: JG
authors.lastname: Goh
authors.affiliation: Temasek Life Sciences Laboratory (TLL), 1 Research Link, National University of Singapore, Singapore 117604, Singapore.
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.affiliation: Temasek Life Sciences Laboratory (TLL), 1 Research Link, National University of Singapore, Singapore 117604, Singapore.
authors.forename: Wan-Yi
authors.initials: WY
authors.lastname: Wee
authors.affiliation: 1] Temasek Life Sciences Laboratory (TLL), 1 Research Link, National University of Singapore, Singapore 117604, Singapore [2] Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Science Drive 4, Singapore 117543, Singapore.
authors.forename: Jiangbo
authors.initials: J
authors.lastname: Huang
authors.affiliation: 1] Temasek Life Sciences Laboratory (TLL), 1 Research Link, National University of Singapore, Singapore 117604, Singapore [2] Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Science Drive 4, Singapore 117543, Singapore.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Ito
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D035264:Flowers / Q000201:enzymology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006657:Histones / Q000378:metabolism; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics / Q000378:metabolism*; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism*; D008745:Methylation
chemical_list: D029681:Arabidopsis Proteins; C418902:At5g10140 protein, Arabidopsis; D006657:Histones; D026161:MADS Domain Proteins; C000602336:JMJ30 protein, Arabidopsis; C000602337:JMJ32 protein, Arabidopsis; D056484:Jumonji Domain-Containing Histone Demethylases