title: Comparison of five different C18 HPLC analytical columns for the analysis of ochratoxin A in different matrices.
abstract: The performance of five different C18 chromatography analytical columns with different lengths, particle sizes and porosities were compared for analysis of ochratoxin A (OTA) in fungal cultures and raisin samples. Chromatographic parameters including retention time, limit of detection, limit of quantification, number of theoretical plates and reduced plate height were obtained and compared. This showed that, compared with traditional columns, shorter ones (100 and 75mm×4.6mm) with 2.7μm solid core particles are suitable for analysis of OTA in different matrices and allows a reduction of the total analysis time by approximately 50% without any detrimental effect on performance. This leads to significant reduction in analysis costs by savings in use of organic solvents and increasing the total number of analyses per day. The capability of these columns for analyzing samples, from different matrices, was assessed by analyzing OTA-contaminated samples from cultures of Aspergillus westerdijkiae and Aspergillus niger grown on a defined nutritional media (yeast extract sucrose agar) and from natural and OTA spiked raisins.
authors.affiliation: Applied Mycology Group, Cranfield Soil and AgriFood Institute, Cranfield University, Cranfield, Bedfordshire MK43 0AL, UK; Department of Food Toxicology and Contaminants, National Research Centre, Dokki, Cairo 12622, Egypt.
authors.forename: Y
authors.initials: Y
authors.lastname: Sultan
authors.affiliation: Applied Mycology Group, Cranfield Soil and AgriFood Institute, Cranfield University, Cranfield, Bedfordshire MK43 0AL, UK.
authors.forename: N
authors.initials: N
authors.lastname: Magan
authors.affiliation: Applied Mycology Group, Cranfield Soil and AgriFood Institute, Cranfield University, Cranfield, Bedfordshire MK43 0AL, UK. Electronic address: a.medinavaya@cranfield.ac.uk.
authors.forename: A
authors.initials: A
authors.lastname: Medina
mesh_terms: D001230:Aspergillus / Q000254:growth & development; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D003365:Costs and Cost Analysis; D017077:Culture Media, Conditioned / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D009793:Ochratoxins / Q000032:analysis*; D012680:Sensitivity and Specificity; D013997:Time Factors; D027843:Vitis / Q000737:chemistry
chemical_list: D017077:Culture Media, Conditioned; D009793:Ochratoxins; C025589:ochratoxin A
keywords: Column length; Ochratoxin A; Particle size; Poroshell; Raisins; Solid core particles