title: Inhibition of cellular proliferation and enhancement of hydrogen peroxide production in fibrosarcoma cell line by weak radio frequency magnetic fields.
abstract: This study presents experimental data for the effects of weak radio frequency (RF) magnetic fields on hydrogen peroxide (H2O2) production and cellular growth rates of fibrosarcoma HT1080 cells in vitro. Cells were exposed either to 45 µT static magnetic fields (SMFs)-oriented vertical to the plane of growth or to SMFs combined with weak 5 and 10 MHz RF magnetic fields of 10 µTRMS intensity perpendicular to the static field. Cell numbers were reduced up to 30% on Day 2 for the cells exposed to the combination of SMF and a 10 MHz RF magnetic field compared with the SMF control cells. In addition, cells exposed to 10 MHz RF magnetic fields for 8 h increased H2O2 production by 55%. The results demonstrate an overall magnetic field-induced biological effect that shows elevated H2O2 levels with accompanying decrease in cellular growth rates.
authors.affiliation: Institute of Chemistry and Biological Physical Chemistry-Faculty of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina; Mechanical Engineering Department, University of Nevada Reno, Reno, Nevada.
authors.forename: Pablo R
authors.initials: PR
authors.lastname: Castello
authors.forename: Iain
authors.initials: I
authors.lastname: Hill
authors.forename: Frank
authors.initials: F
authors.lastname: Sivo
authors.forename: Lucas
authors.initials: L
authors.lastname: Portelli
authors.forename: Frank
authors.initials: F
authors.lastname: Barnes
authors.forename: Robert
authors.initials: R
authors.lastname: Usselman
authors.forename: Carlos F
authors.initials: CF
authors.lastname: Martino
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000528:radiation effects; D004574:Electromagnetic Fields; D005354:Fibrosarcoma / Q000473:pathology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D011846:Radio Waves; D013997:Time Factors
chemical_list: D006861:Hydrogen Peroxide
keywords: cancer cells; cellular growth curves; hydrogen peroxide; magnetic field effects; radical pair mechanism; radio frequency