title: [Effect of bone marrow mesenchymal stem cells on lipopolysacharide-induced secretion of inflammatory cytokines in rat macrophages in vitro].
abstract: OBJECTIVE
  To investigate the effect of bone marrow mesenchymal stem cells (BMSCs) on secretion of inflammatory cytokines in macrophages stimulated by lipopolysacharide (LPS).
  
  
  METHODS
  Rat BMSCs and macrophages were isolated, cultured, and identified. The BMSCs and macrophages, cultured alone or in co-culture, were treated with LPS or PBS or without treatment and tested for interleukin-10 (IL-10) and tumor necrosis factor-α (TNF-α) concentrations in the supernatants at 1, 3, 5, 7, 12, and 24 h after the treatment using enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Exposure to LPS caused significantly increased IL-10 and TNF-α concentrations in the supernatant of cultured macrophages but not in BMSC culture. Macrophages co-cultured with BMSCs showed significantly lowered IL-10 and TNF-α secretions in response to LPS exposure as compared with the macrophages cultured alone.
  
  
  CONCLUSIONS
  BMSCs can reduce LPS-induced secretion of inflammatory cytokines by the macrophages to ameliorate inflammatory reactions.
authors.affiliation: 1Southern Medical University, Guangzhou 510515, China; 2Department of Burn Surgery, Guangdong General Hospital/Guangdong Academy of Medical Sciences, Guangzhou 510080, China. E-mail: wanghuanli1012@126.com.
authors.forename: Huanli
authors.initials: H
authors.lastname: Wang
authors.forename: Bing
authors.initials: B
authors.lastname: Xiong
authors.forename: Huade
authors.initials: H
authors.lastname: Chen
authors.forename: Wen
authors.initials: W
authors.lastname: Lai
authors.forename: Shaoyi
authors.initials: S
authors.lastname: Zheng
authors.forename: Huining
authors.initials: H
authors.lastname: Bian
authors.forename: Zuan
authors.initials: Z
authors.lastname: Liu
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Huang
authors.forename: Chuanwei
authors.initials: C
authors.lastname: Sun
authors.forename: Lianghua
authors.initials: L
authors.lastname: Ma
authors.forename: Hanhua
authors.initials: H
authors.lastname: Li
authors.forename: Lijun
authors.initials: L
authors.lastname: Wei
authors.forename: Hanxi
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D007249:Inflammation; D016753:Interleukin-10 / Q000378:metabolism*; D008070:Lipopolysaccharides; D008264:Macrophages / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10