title: Sperm morphological normality under high magnification predicts laboratory and clinical outcomes in couples undergoing ICSI.
abstract: The objective of this study was to evaluate whether 'motile sperm organelle morphology examination' (MSOME) is correlated with the outcome of ICSI. A total of 14400 spermatozoa from 72 couples undergoing ICSI were analysed by MSOME (x6600) and graded into four groups: grade I, normal form and no vacuoles; grade II, normal form and lesser than or equal to 2 small vacuoles; grade III, normal form greater than 2 small vacuoles or at least one large vacuole and grade IV, large vacuole and abnormal head shapes or other abnormalities. The correlations between the proportion of morphologically normal spermatozoa (grade I + II) and ICSI outcomes were assessed. The proportion of grade I+ II spermatozoa was lower in patients with oligoasthenoteratozoospermia (OAT) compared to patients with other types of semen alterations (10.6% vs. 17.0%, p = 0.001). The proportion of grade I+ II spermatozoa was positively correlated with blastocyst formation (S = 8.31, R(2):13.5%, p = 0.014) and implantation rates (S = 8.32, R(2): 7.9%, p = 0.030). The proportion of grade I + II spermatozoa was higher in patients with ongoing pregnancy in comparison with those who had a miscarriage (23.2% vs. 10.8%, p = 0.007). Sperm morphological normality was lower in oligoasthenoteratozoospermia patients but correlated with blastocyst formation, implantation and miscarriage rates in couples undergoing ICSI. MSOME may be valuable in predicting ICSI outcomes.
authors.affiliation: Fertility - Centro de Fertilização Assistida , São Paulo - SP , Brazil.
authors.forename: Livia
authors.initials: L
authors.lastname: Vingris
authors.forename: Amanda Souza
authors.initials: AS
authors.lastname: Setti
authors.forename: Daniela Paes
authors.initials: DP
authors.lastname: De Almeida Ferreira Braga
authors.forename: Rita
authors.initials: R
authors.lastname: De Cassia Savio Figueira
authors.forename: Assumpto
authors.initials: A
authors.lastname: Iaconelli
authors.forename: Edson
authors.initials: E
authors.lastname: Borges
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011247:Pregnancy; D011379:Prognosis; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic / Q000706:statistics & numerical data*; D013094:Spermatozoa / Q000473:pathology*
keywords: ICSI; MSOME; semen analysis; sperm; vacuole