title: Transcriptomic response of Enterococcus faecalis V583 to low hydrogen peroxide levels.
abstract: Enterococcus faecalis is a Gram-positive commensal bacterium inhabiting the gastrointestinal tracts of human and other mammals, but is also increasingly recognized as an opportunistic human pathogen. Oxidative stress is one of the major challenges encountered by enterococci, both in their natural environment and during infection. In this paper, we evaluated the transcriptomic response of E. faecalis to oxidative stress, and showed that transcript abundance was reduced for 93 genes and increased for 39 genes during growth in medium containing 1.75 mM H2O2. The presence of hydrogen peroxide affected several metabolic pathways, including a large decrease in ethanolamine utilization and methylglyoxal metabolism, and an increase in transcript abundance for several transport systems. In particular, four operons encoding iron transporters appeared highly induced. By contrast, in our experimental conditions, the expression of most of the genes known to be involved in the enterococcal response to oxidative stress, did not appear significantly altered.
authors.affiliation: Unité de Recherche Risques Microbiens (U2RM), Equipe Stress Virulence, Université de Caen Basse-Normandie, 14032, Caen, France.
authors.forename: Xue
authors.initials: X
authors.lastname: Yan
authors.forename: Aurélie
authors.initials: A
authors.lastname: Budin-Verneuil
authors.forename: Nicolas
authors.initials: N
authors.lastname: Verneuil
authors.forename: Michael S
authors.initials: MS
authors.lastname: Gilmore
authors.forename: Sébastien
authors.initials: S
authors.lastname: Artigaud
authors.forename: Yanick
authors.initials: Y
authors.lastname: Auffray
authors.forename: Vianney
authors.initials: V
authors.lastname: Pichereau
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics; D001692:Biological Transport; D004305:Dose-Response Relationship, Drug; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D019856:Ethanolamine / Q000378:metabolism; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D053263:Gene Regulatory Networks; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D058977:Molecular Sequence Annotation; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics; D011765:Pyruvaldehyde / Q000378:metabolism; D059467:Transcriptome
chemical_list: D019856:Ethanolamine; D011765:Pyruvaldehyde; D006861:Hydrogen Peroxide