title: HyperBIRD: a sensitivity-enhanced approach to collecting homonuclear-decoupled proton NMR spectra.
abstract: Samples prepared following dissolution dynamic nuclear polarization (DNP) enable the detection of NMR spectra from low-γ nuclei with outstanding sensitivity, yet have limited use for the enhancement of abundant species like (1)H nuclei. Small- and intermediate-sized molecules, however, show strong heteronuclear cross-relaxation effects: spontaneous processes with an inherent isotopic selectivity, whereby only the (13)C-bonded protons receive a polarization enhancement. These effects are here combined with a recently developed method that delivers homonuclear-decoupled (1)H spectra in natural abundance samples based on heteronuclear couplings to these same, (13)C-bonded nuclei. This results in the HyperBIRD methodology; a single-shot combination of these two effects that can simultaneously simplify and resolve complex, congested (1)H NMR spectra with many overlapping spin multiplets, while achieving 50-100 times sensitivity enhancements over conventional thermal counterparts.
authors.affiliation: Chemical Physics Department, Weizmann Institute of Science, 76100 Rehovot (Israel) http://www.weizmann.ac.il/chemphys/Frydman_group/home.html; Current address: Department of Chemistry and Francis Bitter Magnet Laboratory, Massachusetts Institute of Technology, 150 Albany St, NW14, Cambridge, MA 02139 (USA).
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Donovan
authors.forename: Lucio
authors.initials: L
authors.lastname: Frydman
mesh_terms: D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods*
keywords: NMR spectroscopy; homonuclear decoupling; hyperpolarization; structure elucidation