title: Thermophilic hydrogen production from sludge pretreated by thermophilic bacteria: analysis of the advantages of microbial community and metabolism.
abstract: In this study, the effects of thermophilic bacteria pretreatment and elevated fermentation temperature on hydrogen production from sludge were examined. The highest hydrogen yield of 19.9mlH2g(-1) VSS was achieved at 55°C by using pretreated sludge, which was 48.6% higher than raw sludge without pretreatment, and 28.39% higher than when fermented at 35°C. To explore the internal factors of this superior hydrogen production performance, the microbial community and the metabolism analysis were performed by using high-throughput sequencing and excitation-emission matrix. The pretreated sludge showed better utilization of dissolved organic matter and less inhibition of metabolism, especially at thermophilic condition. The 454 sequencing data indicated that microbial abundance was distinctly reduced and extremely high proportion of hydrogen-producing bacteria was found in the thermophilic community (Thermoanaerobacterium accounted for 93.75%). Thus, the pretreated sludge and thermophilic condition showed significant advantages in the hydrogen production using waste sludge as substrate.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
authors.forename: He-Shan
authors.initials: HS
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China. Electronic address: guowanqian@hit.edu.cn.
authors.forename: Wan-Qian
authors.initials: WQ
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
authors.forename: Xiao-Chi
authors.initials: XC
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
authors.forename: Juan-Shan
authors.initials: JS
authors.lastname: Du
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
authors.forename: Xian-Jiao
authors.initials: XJ
authors.lastname: Zhou
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
authors.forename: Nan-Qi
authors.initials: NQ
authors.lastname: Ren
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D001411:Geobacillus stearothermophilus / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism*; D006859:Hydrogen / Q000302:isolation & purification* / Q000378:metabolism*; D059013:Microbial Consortia / Q000502:physiology*; D012722:Sewage / Q000737:chemistry* / Q000382:microbiology*; D013045:Species Specificity
chemical_list: D012722:Sewage; D006859:Hydrogen
keywords: High-throughput sequencing; Microbial community; Sludge; Thermophilic bacteria; Thermophilic hydrogen production