title: Tomato Male sterile 1035 is essential for pollen development and meiosis in anthers.
abstract: Male fertility in flowering plants depends on proper cellular differentiation in anthers. Meiosis and tapetum development are particularly important processes in pollen production. In this study, we showed that the tomato male sterile (ms10(35)) mutant of cultivated tomato (Solanum lycopersicum) exhibited dysfunctional meiosis and an abnormal tapetum during anther development, resulting in no pollen production. We demonstrated that Ms10(35) encodes a basic helix-loop-helix transcription factor that is specifically expressed in meiocyte and tapetal tissue from pre-meiotic to tetrad stages. Transgenic expression of the Ms10(35) gene from its native promoter complemented the male sterility of the ms10(35) mutant. In addition, RNA-sequencing-based transcriptome analysis revealed that Ms10(35) regulates 246 genes involved in anther development processes such as meiosis, tapetum development, cell-wall degradation, pollen wall formation, transport, and lipid metabolism. Our results indicate that Ms10(35) plays key roles in regulating both meiosis and programmed cell death of the tapetum during microsporogenesis.
authors.affiliation: Department of Plant Science and Plant Genomics and Breeding Institute, College of Agriculture and Life Science, Seoul National University, 599 Gwanak-ro Gwank-gu, Seoul 151-921, Republic of Korea Plant Genomics and Breeding Institute, College of Agricultural Sciences, Seoul National University, 599 Gwanak-ro Gwank-gu, Seoul 151-921, Republic of Korea.
authors.forename: Hee-Jin
authors.initials: HJ
authors.lastname: Jeong
authors.affiliation: Department of Plant Science and Plant Genomics and Breeding Institute, College of Agriculture and Life Science, Seoul National University, 599 Gwanak-ro Gwank-gu, Seoul 151-921, Republic of Korea Plant Genomics and Breeding Institute, College of Agricultural Sciences, Seoul National University, 599 Gwanak-ro Gwank-gu, Seoul 151-921, Republic of Korea.
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Kang
authors.affiliation: College of Life Science, Qingdao Agricultural University, Qingdao 266-109, PR China.
authors.forename: Meiai
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Plant Science and Plant Genomics and Breeding Institute, College of Agriculture and Life Science, Seoul National University, 599 Gwanak-ro Gwank-gu, Seoul 151-921, Republic of Korea Plant Genomics and Breeding Institute, College of Agricultural Sciences, Seoul National University, 599 Gwanak-ro Gwank-gu, Seoul 151-921, Republic of Korea.
authors.forename: Jin-Kyung
authors.initials: JK
authors.lastname: Kwon
authors.affiliation: National Institute of Horticultural and Herbal Science, Suwon 440-310, Republic of Korea.
authors.forename: Hak-Soon
authors.initials: HS
authors.lastname: Choi
authors.affiliation: Department of Plant Science and Plant Genomics and Breeding Institute, College of Agriculture and Life Science, Seoul National University, 599 Gwanak-ro Gwank-gu, Seoul 151-921, Republic of Korea.
authors.forename: Jung Hwan
authors.initials: JH
authors.lastname: Bae
authors.affiliation: Department of Plant Science and Plant Genomics and Breeding Institute, College of Agriculture and Life Science, Seoul National University, 599 Gwanak-ro Gwank-gu, Seoul 151-921, Republic of Korea Plant Genomics and Breeding Institute, College of Agricultural Sciences, Seoul National University, 599 Gwanak-ro Gwank-gu, Seoul 151-921, Republic of Korea.
authors.forename: Hyun-Ah
authors.initials: HA
authors.lastname: Lee
authors.affiliation: School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Joung
authors.affiliation: Department of Plant Science and Plant Genomics and Breeding Institute, College of Agriculture and Life Science, Seoul National University, 599 Gwanak-ro Gwank-gu, Seoul 151-921, Republic of Korea Plant Genomics and Breeding Institute, College of Agricultural Sciences, Seoul National University, 599 Gwanak-ro Gwank-gu, Seoul 151-921, Republic of Korea.
authors.forename: Doil
authors.initials: D
authors.lastname: Choi
authors.affiliation: Department of Plant Science and Plant Genomics and Breeding Institute, College of Agriculture and Life Science, Seoul National University, 599 Gwanak-ro Gwank-gu, Seoul 151-921, Republic of Korea Plant Genomics and Breeding Institute, College of Agricultural Sciences, Seoul National University, 599 Gwanak-ro Gwank-gu, Seoul 151-921, Republic of Korea bk54@snu.ac.kr.
authors.forename: Byoung-Cheorl
authors.initials: BC
authors.lastname: Kang
mesh_terms: D000595:Amino Acid Sequence; D000705:Anaphase; D051792:Basic Helix-Loop-Helix Proteins; D059447:Cell Cycle Checkpoints; D002473:Cell Wall / Q000378:metabolism; D003001:Cloning, Molecular; D015536:Down-Regulation; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D050356:Lipid Metabolism / Q000235:genetics; D018551:Solanum lycopersicum / Q000166:cytology* / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D008540:Meiosis / Q000235:genetics*; D008954:Models, Biological; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D051479:Plant Infertility / Q000235:genetics*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D011058:Pollen / Q000166:cytology* / Q000254:growth & development* / Q000648:ultrastructure; D017423:Sequence Analysis, RNA
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D010940:Plant Proteins
keywords: Anther; male sterility; meiosis; tapetum; tomato (Solanum lycopersicum).