title: Prokaryotic phylogenetic diversity of Hungarian deep subsurface geothermal well waters.
abstract: Geothermal wells characterized by thermal waters warmer than 30°C can be found in more than 65% of the area of Hungary. The examined thermal wells located nearby Szarvas are used for heating industrial and agricultural facilities because of their relatively high hydrocarbon content. The aim of this study was to reveal the prokaryotic community structure of the water of SZR18, K87 and SZR21 geothermal wells using molecular cloning methods and Denaturing Gradient Gel Electrophoresis (DGGE). Water samples from the outflow pipes were collected in 2012 and 2013. The phylogenetic distribution of archaeal molecular clones was very similar in each sample, the most abundant groups belonged to the genera Methanosaeta, Methanothermobacter and Thermofilum. In contrast, the distribution of bacterial molecular clones was very diverse. Many of them showed the closest sequence similarities to uncultured clone sequences from similar thermal environments. From the water of the SZR18 well, phylotypes closely related to genera Fictibacillus and Alicyclobacillus (Firmicutes) were only revealed, while the bacterial diversity of the K87 well water was much higher. Here, the members of the phyla Thermodesulfobacteria, Proteobacteria, Nitrospira, Chlorobi, OP1 and OPB7 were also detected besides Firmicutes.
authors.affiliation: Eötvös Loránd University Department of Microbiology Pázmány P. sétány 1/C H-1117 Budapest Hungary.
authors.forename: Andrea
authors.initials: A
authors.lastname: Németh
authors.affiliation: Eötvös Loránd University Department of Microbiology Pázmány P. sétány 1/C H-1117 Budapest Hungary.
authors.forename: Barbara
authors.initials: B
authors.lastname: Szirányi
authors.affiliation: Eötvös Loránd University Department of Microbiology Pázmány P. sétány 1/C H-1117 Budapest Hungary.
authors.forename: Gergely
authors.initials: G
authors.lastname: Krett
authors.affiliation: Research Institute for Fisheries, Aquaculture and Irrigation Anna-liget 8 H-5540 Szarvas Hungary.
authors.forename: Endre
authors.initials: E
authors.lastname: Janurik
authors.affiliation: Research Institute for Fisheries, Aquaculture and Irrigation Anna-liget 8 H-5540 Szarvas Hungary.
authors.forename: Tünde
authors.initials: T
authors.lastname: Kosáros
authors.affiliation: Research Institute for Fisheries, Aquaculture and Irrigation Anna-liget 8 H-5540 Szarvas Hungary.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Pekár
authors.affiliation: Eötvös Loránd University Department of Microbiology Pázmány P. sétány 1/C H-1117 Budapest Hungary.
authors.forename: Károly
authors.initials: K
authors.lastname: Márialigeti
authors.affiliation: Eötvös Loránd University Department of Microbiology Pázmány P. sétány 1/C H-1117 Budapest Hungary.
authors.forename: Andrea K
authors.initials: AK
authors.lastname: Borsodi
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D045482:Hot Springs / Q000382:microbiology*; D006814:Hungary; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA gene; denaturing gradient gel electrophoresis; geothermal well water; molecular cloning; phylogenetic diversity