title: PTEN redundancy: overexpressing lpten, a homolog of Dictyostelium discoideum ptenA, the ortholog of human PTEN, rescues all behavioral defects of the mutant ptenA-.
abstract: Mutations in the tumor suppressor gene PTEN are associated with a significant proportion of human cancers. Because the human genome also contains several homologs of PTEN, we considered the hypothesis that if a homolog, functionally redundant with PTEN, can be overexpressed, it may rescue the defects of a PTEN mutant. We have performed an initial test of this hypothesis in the model system Dictyostelium discoideum, which contains an ortholog of human PTEN, ptenA. Deletion of ptenA results in defects in motility, chemotaxis, aggregation and multicellular morphogenesis. D. discoideum also contains lpten, a newly discovered homolog of ptenA. Overexpressing lpten completely rescues all developmental and behavioral defects of the D. discoideum mutant ptenA-. This hypothesis must now be tested in human cells.
authors.affiliation: Monoclonal Antibody Research Institute and Developmental Studies Hybridoma Bank, Department of Biology, The University of Iowa, Iowa City, Iowa, United States of America.
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Lusche
authors.affiliation: Monoclonal Antibody Research Institute and Developmental Studies Hybridoma Bank, Department of Biology, The University of Iowa, Iowa City, Iowa, United States of America.
authors.forename: Deborah
authors.initials: D
authors.lastname: Wessels
authors.affiliation: Monoclonal Antibody Research Institute and Developmental Studies Hybridoma Bank, Department of Biology, The University of Iowa, Iowa City, Iowa, United States of America.
authors.forename: Nicole A
authors.initials: NA
authors.lastname: Richardson
authors.affiliation: Monoclonal Antibody Research Institute and Developmental Studies Hybridoma Bank, Department of Biology, The University of Iowa, Iowa City, Iowa, United States of America.
authors.forename: Kanoe B
authors.initials: KB
authors.lastname: Russell
authors.affiliation: Monoclonal Antibody Research Institute and Developmental Studies Hybridoma Bank, Department of Biology, The University of Iowa, Iowa City, Iowa, United States of America.
authors.forename: Brett M
authors.initials: BM
authors.lastname: Hanson
authors.affiliation: Monoclonal Antibody Research Institute and Developmental Studies Hybridoma Bank, Department of Biology, The University of Iowa, Iowa City, Iowa, United States of America.
authors.forename: Benjamin A
authors.initials: BA
authors.lastname: Soll
authors.affiliation: Monoclonal Antibody Research Institute and Developmental Studies Hybridoma Bank, Department of Biology, The University of Iowa, Iowa City, Iowa, United States of America.
authors.forename: Benjamin H
authors.initials: BH
authors.lastname: Lin
authors.affiliation: Monoclonal Antibody Research Institute and Developmental Studies Hybridoma Bank, Department of Biology, The University of Iowa, Iowa City, Iowa, United States of America.
authors.forename: David R
authors.initials: DR
authors.lastname: Soll
mesh_terms: D001483:Base Sequence; D002633:Chemotaxis / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D000242:Cyclic AMP / Q000494:pharmacology; D016054:DNA, Protozoan / Q000235:genetics*; D004023:Dictyostelium / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D055786:Gene Knockout Techniques; D017125:Genes, Protozoan; D006801:Humans; D008969:Molecular Sequence Data; D051059:PTEN Phosphohydrolase / Q000502:physiology; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013045:Species Specificity
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; D000242:Cyclic AMP; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human