title: Effect of prostatic fluid on the quality of fresh and frozen-thawed canine epididymal spermatozoa.
abstract: Canine epididymal spermatozoa have a low freeze-tolerance ability compared with ejaculated spermatozoa, which could arise from the absence of prostatic fluid (PF). Therefore, the purpose of this work was to elucidate the influence of PF on the quality of canine epididymal sperm before and after freezing. Caudae epididymides were retrieved from eight dogs after routine castration. Spermatozoa were released by slicing the tissue and were extended in either Tris solution or PF before freezing. Frozen sperm samples were thawed at 70 °C for 8 seconds in a waterbath. Sperm concentration, motility using computer-assisted sperm analysis, morphology, plasma membrane, acrosome and chromatin integrity were assessed in the fresh sperm samples (after 20 minutes incubation) and at 0 and 4 hours after thawing. Progressive motility, distance straight line, distance average path, average path velocity, curvilinear velocity, straight line velocity, straightness, linearity, wobble, and beat cross frequency were significantly increased after extraction into PF. There was a higher proportion of spermatozoa with DNA damage in the PF treatment group at 4 hours after thawing than in the Tris treatment group (15.8% vs. 6.7%, P < 0.05). These results suggest that the addition of PF to canine spermatozoa activates sperm motility in fresh spermatozoa but has a negative effect on chromatin integrity after freezing-thawing.
authors.affiliation: Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences-SLU, Uppsala, Sweden; Department of Obstetrics and Gynecology, St. Petersburg State Academy of Veterinary Medicine-St.Petersburg, Russia. Electronic address: e.kora@mail.ru.
authors.forename: E
authors.initials: E
authors.lastname: Korochkina
authors.affiliation: Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Johannisson
authors.affiliation: Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences-SLU, Uppsala, Sweden; Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: Lavanya
authors.initials: L
authors.lastname: Goodla
authors.affiliation: Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences-SLU, Uppsala, Sweden.
authors.forename: J M
authors.initials: JM
authors.lastname: Morrell
authors.affiliation: Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences-SLU, Uppsala, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Axner
mesh_terms: D000177:Acrosome; D000818:Animals; D001826:Body Fluids; D018929:Cell Culture Techniques / Q000662:veterinary; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary; D003470:Culture Media; D004285:Dogs; D008297:Male; D011467:Prostate / Q000378:metabolism; D055101:Semen Analysis; D013081:Sperm Motility; D053625:Sperm Retrieval; D013094:Spermatozoa / Q000166:cytology*
chemical_list: D003470:Culture Media
keywords: Canine epididymal spermatozoa; Chromatin integrity; Cryopreservation; Prostatic fluid; Sperm quality