title: Four weeks supplementation with Lactobacillus paracasei subsp. paracasei L. casei W8® shows modest effect on triacylglycerol in young healthy adults.
abstract: The microbiota has been shown to have the potential to affect appetite and blood lipids positively in animal studies. We investigated if four weeks supplementation with Lactobacillus paracasei subsp. paracasei L. casei W8® (L. casei W8) had an effect on subjective appetite sensation, ad libitum energy intake, glucagon-like peptide 1 (GLP-1), glucose and insulin response in humans. Secondarily, we explored potential effects on blood lipids, fatty acids and stearoyl-CoA desaturase-1 (SCD1) activity in humans as well as SCD1 expression in piglets given L. casei W8 for two weeks. 64 healthy participants completed the double-blinded, randomised, controlled, parallel four weeks study with supplementation of L. casei W8 (1010 cfu) or placebo capsules. A meal test was conducted before and after the intervention, where subjective appetite, ad libitum energy intake, GLP-1, glucose and insulin response were measured. Additionally fasting blood lipids and fatty acids concentrations were measured. Sixteen piglets were randomised into two groups: L. casei W8 (1010 cfu/day) as top dressing on morning fed or no treatment. After two weeks piglets were sacrificed and tissue from ileum, jejunum and skeletal muscle were sampled for mRNA analyses of SCD1 expression. Compared to placebo, L. casei W8 did not affect appetite, ad libitum energy intake, GLP-1, glucose and insulin response and total, high-density or low-density lipoprotein cholesterol levels after four weeks intervention. Triacylglycerol decreased in the L. casei W8 group compared to placebo at week 4 (P=0.03). The C16:1n-7/C16:0 ratio, reflecting SCD1 activity, tended to decrease when having L. casei W8 (P=0.06) compared to placebo. Muscle SCD1 expression decreased in piglets supplemented with L. casei W8 compared to control. In conclusion, supplementation with L. casei W8 did not affect appetite parameters, glucose or insulin responses; but appear to be able to lower triacylglycerol levels, possibly by reducing its production.
authors.affiliation: Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, Nørre Alle 51, 2200 Copenhagen N, Denmark.
authors.forename: A T
authors.initials: AT
authors.lastname: Bjerg
authors.affiliation: Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, Nørre Alle 51, 2200 Copenhagen N, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Kristensen
authors.affiliation: Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, Nørre Alle 51, 2200 Copenhagen N, Denmark.
authors.forename: C
authors.initials: C
authors.lastname: Ritz
authors.affiliation: Department of Kinesiology, University of Waterloo, 200 University Avenue West, Waterloo, ON N2L 3G1, Canada.
authors.forename: K D
authors.initials: KD
authors.lastname: Stark
authors.affiliation: The Novo Nordisk Foundation Center of Basic Metabolism Research, Department of Biomedical Sciences, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen N, Denmark.
authors.forename: J J
authors.initials: JJ
authors.lastname: Holst
authors.affiliation: Chr. Hansen A/S, Bøge Alle 10-12, 2970 Hørsholm, Denmark.
authors.forename: T D
authors.initials: TD
authors.lastname: Leser
authors.affiliation: Chr. Hansen A/S, Bøge Alle 10-12, 2970 Hørsholm, Denmark.
authors.forename: A
authors.initials: A
authors.lastname: Wellejus
authors.affiliation: Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, Nørre Alle 51, 2200 Copenhagen N, Denmark.
authors.forename: A
authors.initials: A
authors.lastname: Astrup
mesh_terms: D000328:Adult; D000818:Animals; D001066:Appetite / Q000187:drug effects*; D001786:Blood Glucose; D020869:Gene Expression Profiling; D052216:Glucagon-Like Peptide 1 / Q000096:biosynthesis / Q000097:blood; D006801:Humans; D007082:Ileum / Q000473:pathology; D007328:Insulin / Q000097:blood; D007583:Jejunum / Q000473:pathology; D007780:Lacticaseibacillus casei / Q000254:growth & development* / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000473:pathology; D010919:Placebos / Q000008:administration & dosage; D019936:Probiotics / Q000008:administration & dosage*; D013230:Stearoyl-CoA Desaturase / Q000097:blood; D013552:Swine; D014280:Triglycerides / Q000097:blood*
chemical_list: D001786:Blood Glucose; D007328:Insulin; D010919:Placebos; D014280:Triglycerides; D052216:Glucagon-Like Peptide 1; D013230:Stearoyl-CoA Desaturase
keywords: Lactobacillus paracasei; SCD1; appetite; probiotics; triacylglycerol