title: A novel peptide of endothelin family, 31 amino-acid length endothelin in patients with acute myocardial infarction.
abstract: Human chymase converts big endothelin (ET)-1 to 31-amino acid length ET-1 {ET-1(1-31)} that also possesses a potent vasoconstrictive action. In addition, ET-1(1-31) is an intermediate peptide, which is then readily transformed to mature ET-1 by the neutral endopeptidase 24-11. To investigate the relevance of pathophysiology of ET-1(1-31) in vivo, we have developed specific sandwich-type, enzyme-linked immunosorbent assay to measure the plasma concentration of ET-1(1-31) in healthy volunteers and patients with myocardial infarction. The plasma concentrations of ET-1(1-31) in healthy volunteers were 24.8 ± 5.2 pg/ml (n=11). ET-1(1-31) concentration in plasma was elevated in patients with acute myocardial infarction, and its elevation was several times higher and lasted longer than that of ET-1. In addition, tissue concentration of ET-1(1-31) in the myocardium from a patient with acute myocardial infarction was extremely high (12729.8 ± 2617.7 pg/mg protein). These results suggest that ET-1(1-31) may play some pathological roles in the remodeling, especially in sites where inflammatory cells produced a large amount of proteases, such as myocardial infarction.
authors.affiliation: Department of Nutrition and Metabolism, the University of Tokushima Graduate School.
authors.forename: Makiko
authors.initials: M
authors.lastname: Oka
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Niwa
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Mawatari
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Hiasa
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakaya
mesh_terms: D000328:Adult; D000368:Aged; D019332:Endothelin-1 / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis*
chemical_list: D019332:Endothelin-1; D010446:Peptide Fragments