title: Impact of LPS-induced cardiomyoblast cell apoptosis inhibited by earthworm extracts.
abstract: Dilong is an earthworm extract with a dense nutritional content, widely used in Chinese herbal medicine to remove stasis and stimulate wound healing. Earthworm extracts are traditionally used by indigenous people throughout the world. How this Dilong inhibits Lipopolysaccharide (LPS)-induced cardiomyoblast cell apoptosis is still unclear. This study investigates the Dilong extract effect on LPS-induced apoptosis in H9c2 cardiomyoblast cells. LPS (1 μg/ml) administration for 24 h induced apoptosis in H9c2 cells. Cell apoptosis was detected using MTT, LDH, TUNEL assay and JC-1 staining. Western blot analysis was used to detect pro-apoptotic and anti-apoptotic proteins. Dilong extract totally blocked the LPS impact, leading to the activation of anti-apoptotic proteins, Bcl-2 and Bcl-xL, stabilized the mitochondria membrane and down-regulated the extrinsic and intrinsic pro-apoptotic proteins, TNF-α, active caspase-8, t-Bid, Bax, active caspase-9 and active caspase-3. Dilong could potentially serve as a cardio protective agent against LPS-induced H9c2 cardiomyoblast cell apoptosis.
authors.affiliation: Division of Cardiovascular Surgery, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Ping-Chun
authors.initials: PC
authors.lastname: Li
authors.forename: Yun-Chen
authors.initials: YC
authors.lastname: Tien
authors.forename: Cecilia Hsuan
authors.initials: CH
authors.lastname: Day
authors.forename: Peiying
authors.initials: P
authors.lastname: Pai
authors.forename: Wei-Wen
authors.initials: WW
authors.lastname: Kuo
authors.forename: Tung-Sheng
authors.initials: TS
authors.lastname: Chen
authors.forename: Chia-Hua
authors.initials: CH
authors.lastname: Kuo
authors.forename: Chang-Hai
authors.initials: CH
authors.lastname: Tsai
authors.forename: Da-Tong
authors.initials: DT
authors.lastname: Ju
authors.forename: Chih-Yang
authors.initials: CY
authors.lastname: Huang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000473:pathology; D009835:Oligochaeta; D014020:Tissue Extracts / Q000494:pharmacology*
chemical_list: D008070:Lipopolysaccharides; D014020:Tissue Extracts