title: Mitochondrial metabolomics using high-resolution Fourier-transform mass spectrometry.
abstract: High-resolution Fourier-transform mass spectrometry (FTMS) provides important advantages in studies of metabolism because more than half of common intermediary metabolites can be measured in 10 min with minimal pre-detector separation and without ion dissociation. This capability allows unprecedented opportunity to study complex metabolic systems, such as mitochondria. Analysis of mouse liver mitochondria using FTMS with liquid chromatography shows that sex and genotypic differences in mitochondrial metabolism can be readily distinguished. Additionally, differences in mitochondrial function are readily measured, and many of the mitochondria-related metabolites are also measurable in plasma. Thus, application of high-resolution mass spectrometry provides an approach for integrated studies of complex metabolic processes of mitochondrial function and dysfunction in disease.
authors.affiliation: Clinical Biomarkers Laboratory, Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Emory University, 615 Michael Street, Atlanta, GA, 30322, USA.
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Go
authors.forename: Karan
authors.initials: K
authors.lastname: Uppal
authors.forename: Douglas I
authors.initials: DI
authors.lastname: Walker
authors.forename: ViLinh
authors.initials: V
authors.lastname: Tran
authors.forename: Lauriane
authors.initials: L
authors.lastname: Dury
authors.forename: Frederick H
authors.initials: FH
authors.lastname: Strobel
authors.forename: Hélène
authors.initials: H
authors.lastname: Baubichon-Cortay
authors.forename: Kurt D
authors.initials: KD
authors.lastname: Pennell
authors.forename: James R
authors.initials: JR
authors.lastname: Roede
authors.forename: Dean P
authors.initials: DP
authors.lastname: Jones
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D005583:Fourier Analysis; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D008930:Mitochondria, Liver / Q000378:metabolism*