title: Exposure to endocrine disrupters and nuclear receptor gene expression in infertile and fertile women from different Italian areas.
abstract: Within the PREVIENI project, infertile and fertile women were enrolled from metropolitan, urban and rural Italian areas. Blood/serum levels of several endocrine disrupters (EDs) (perfluorooctane sulfonate, PFOS; perfluorooctanoic acid, PFOA; di-2-ethylhexyl-phthalate, DEHP; mono-(2-ethylhexyl)-phthalate, MEHP; bisphenol A, BPA) were evaluated concurrently with nuclear receptors (NRs) gene expression levels (ERa, ERb, AR, AhR, PPARg, PXR) in peripheral blood mononuclear cells (PBMCs). Infertile women from the metropolitan area displayed significantly higher levels of: BPA compared to fertile women (14.9 vs. 0.5 ng/mL serum); BPA and MEHP compared to infertile women from urban and rural areas; enhanced expression levels of NRs, except PPARg. Infertile women from urban and rural areas had PFOA levels significantly higher than those from metropolitan areas. Our study indicates the relevance of the living environment when investigating the exposure to EDs and the modulation of the NR panel in PBMC as a suitable biomarker of the effect, to assess the EDs impact on reproductive health.
authors.affiliation: Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità, Roma 00161, Italy. cinzia.larocca@iss.it.
authors.forename: Cinzia
authors.initials: C
authors.lastname: La Rocca
authors.affiliation: Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità, Roma 00161, Italy. sabrina.tait@iss.it.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Tait
authors.affiliation: Department of Physical, Earth and Environmental Sciences, University of Siena, Siena 53100, Italy. guerranticri@unisi.it.
authors.forename: Cristiana
authors.initials: C
authors.lastname: Guerranti
authors.affiliation: Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità, Roma 00161, Italy. luca.busani@iss.it.
authors.forename: Luca
authors.initials: L
authors.lastname: Busani
authors.affiliation: Department of Obstetric and Gynecological Sciences and Urological Sciences, University of Roma Sapienza, S. Andrea Hospital, Roma 00189, Italy. ciardofrancesca@libero.it.
authors.forename: Francesca
authors.initials: F
authors.lastname: Ciardo
authors.affiliation: Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità, Roma 00161, Italy. brn_001@hotmail.it.
authors.forename: Bruno
authors.initials: B
authors.lastname: Bergamasco
authors.affiliation: Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità, Roma 00161, Italy. laurastecca@hotmail.it.
authors.forename: Laura
authors.initials: L
authors.lastname: Stecca
authors.affiliation: Department of Physical, Earth and Environmental Sciences, University of Siena, Siena 53100, Italy. guido.perra@alice.it.
authors.forename: Guido
authors.initials: G
authors.lastname: Perra
authors.affiliation: Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità, Roma 00161, Italy. francesca.r.mancini@gmail.com.
authors.forename: Francesca Romana
authors.initials: FR
authors.lastname: Mancini
authors.affiliation: Department of Biomedical Sciences and Advanced Therapies, Section of Obstetrics and Gynaecology, University of Ferrara, Ferrara 44121, Italy. roberto.marci@unife.it.
authors.forename: Roberto
authors.initials: R
authors.lastname: Marci
authors.affiliation: Department of Obstetric and Gynecological Sciences and Urological Sciences, University of Roma Sapienza, S. Andrea Hospital, Roma 00189, Italy. bordi.giulia@gmail.com.
authors.forename: Giulia
authors.initials: G
authors.lastname: Bordi
authors.affiliation: Department of Obstetric and Gynecological Sciences and Urological Sciences, University of Roma Sapienza, S. Andrea Hospital, Roma 00189, Italy. donatella.caserta@uniroma1.it.
authors.forename: Donatella
authors.initials: D
authors.lastname: Caserta
authors.affiliation: Department of Physical, Earth and Environmental Sciences, University of Siena, Siena 53100, Italy. focardi@unisi.it.
authors.forename: Silvano
authors.initials: S
authors.lastname: Focardi
authors.affiliation: Department of Obstetric and Gynecological Sciences and Urological Sciences, University of Roma Sapienza, S. Andrea Hospital, Roma 00189, Italy. massimo.moscarini@uniroma1.it.
authors.forename: Massimo
authors.initials: M
authors.lastname: Moscarini
authors.affiliation: Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità, Roma 00161, Italy. alberto.mantovani@iss.it.
authors.forename: Alberto
authors.initials: A
authors.lastname: Mantovani
mesh_terms: D000293:Adolescent; D000328:Adult; D001559:Benzhydryl Compounds / Q000097:blood / Q000633:toxicity; D015415:Biomarkers / Q000097:blood; D002947:Cities; D004051:Diethylhexyl Phthalate / Q000097:blood / Q000633:toxicity; D052244:Endocrine Disruptors / Q000097:blood* / Q000633:toxicity; D004781:Environmental Exposure / Q000032:analysis / Q000706:statistics & numerical data*; D004785:Environmental Pollutants / Q000097:blood* / Q000633:toxicity; D005260:Female; D005466:Fluorocarbons / Q000097:blood*; D006801:Humans; D007247:Infertility, Female / Q000139:chemically induced* / Q000453:epidemiology; D007558:Italy / Q000453:epidemiology; D010636:Phenols / Q000097:blood / Q000633:toxicity; D010795:Phthalic Acids / Q000097:blood / Q000633:toxicity; D018160:Receptors, Cytoplasmic and Nuclear / Q000097:blood / Q000235:genetics; D012424:Rural Population; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D015415:Biomarkers; D004051:Diethylhexyl Phthalate; D052244:Endocrine Disruptors; D004785:Environmental Pollutants; D005466:Fluorocarbons; D010636:Phenols; D010795:Phthalic Acids; D018160:Receptors, Cytoplasmic and Nuclear; C032279:phthalic acid; C006780:bisphenol A; D000098826:Bisphenol A Compounds