title: Polydispersity in size-exclusion chromatography: a stochastic approach.
abstract: We investigate the impact of polydispersity of the sample molecules on the separation process and on the efficiency of size-exclusion chromatography. Polydispersity was integrated into the molecular (stochastic) model of chromatography; the characteristic function, the band profile and the most important moments of the elution profiles were calculated for several kind of pore structures. We investigated the parameters affected by polydispersity on the separation for a number of pore shapes. Our results demonstrate that even a small distribution in the molecular size (i.e. polydispersity) can contribute substantially to the total width of the chromatographic peak. The pure effect of polydispersity can only be investigated via mathematical modeling, because its contribution to an experimental chromatogram cannot be separated from other band-broadening effects.
authors.affiliation: MTA-PTE Molecular Interactions in Separation Science Research Group, Ifjúság útja 6, H-7624 Pécs, Hungary.
authors.forename: Annamária
authors.initials: A
authors.lastname: Sepsey
authors.affiliation: Department of Analytical and Environmental Chemistry and Szentágothai Research Center, University of Pécs, Ifjúság útja 6, H-7624 Pécs, Hungary.
authors.forename: Ivett
authors.initials: I
authors.lastname: Bacskay
authors.affiliation: MTA-PTE Molecular Interactions in Separation Science Research Group, Ifjúság útja 6, H-7624 Pécs, Hungary; Department of Analytical and Environmental Chemistry and Szentágothai Research Center, University of Pécs, Ifjúság útja 6, H-7624 Pécs, Hungary. Electronic address: felinger@ttk.pte.hu.
authors.forename: Attila
authors.initials: A
authors.lastname: Felinger
mesh_terms: D002850:Chromatography, Gel / Q000379:methods*; D008962:Models, Theoretical; D009010:Monte Carlo Method; D013269:Stochastic Processes
keywords: Polydispersity; Size-exclusion chromatography; Stochastic theory