title: A rapid, sensitive and selective electrochemical biosensor with concanavalin A for the preemptive detection of norovirus.
abstract: Norovirus (NoV) is a foodborne pathogen that can cause sporadic and epidemic gastrointestinal diseases. Rapid screening is crucial to promptly identify the presence of NoV and prevent food poisoning. Here, we present a sensitive, selective, and rapid electrochemical biosensor for the detection of NoV. The proposed electrochemical biosensor is composed of a nanostructured gold electrode conjugated with concanavalin A (ConA). ConA functions as a recognition element that selectively captures NoV. Cyclic voltammetry revealed a linear relationship (R(2) = 0.998) between the current and concentration of NoV (in the range of 10(2) and 10(6) copies/mL), with a relatively short assay time (1h) and a good detection limit (35 copies/mL). Additionally, the signals of Hepatitis A and E in the selectively test were found to be only 2.0% and 2.8% of the NoV signal at an identical concentration of 10(3) copies/mL, proving that the electrochemical biosensor has a selectively of approximately 98%. Moreover, the concentration of NoV was measured in a realistic environment, i.e., a sample solution extracted from lettuce, to demonstrate a potential application of the proposed biosensor (LoD = 60 copies/mL).
authors.affiliation: Department of Medical System Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, Republic of Korea.
authors.forename: Sung A
authors.initials: SA
authors.lastname: Hong
authors.affiliation: Korea Basic Science Institute, Daejeon 169-148, Republic of Korea.
authors.forename: Joseph
authors.initials: J
authors.lastname: Kwon
authors.affiliation: Department of Food Science and Technology and Functional Food Research Center, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Duwoon
authors.initials: D
authors.lastname: Kim
authors.affiliation: Department of Medical System Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, Republic of Korea; School of Mechatronics, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, Republic of Korea. Electronic address: syang@gist.ac.kr.
authors.forename: Sung
authors.initials: S
authors.lastname: Yang
mesh_terms: D015374:Biosensing Techniques; D017250:Caliciviridae Infections / Q000175:diagnosis* / Q000821:virology; D003208:Concanavalin A / Q000737:chemistry; D005517:Foodborne Diseases; D006046:Gold / Q000737:chemistry; D006801:Humans; D049329:Nanostructures; D029322:Norovirus / Q000302:isolation & purification* / Q000472:pathogenicity
chemical_list: D003208:Concanavalin A; D006046:Gold
keywords: Alternative protein; Concanavalin A; Electrochemical biosensor; Nanostructured gold electrode; Norovirus