title: Curcumin, a natural antioxidant, acts as a noncompetitive inhibitor of human RNase L in presence of its cofactor 2-5A in vitro.
abstract: Ribonuclease L (RNase L) is an antiviral endoribonuclease of the innate immune system, which is induced and activated by viral infections, interferons, and double stranded RNA (dsRNA) in mammalian cells. Although, RNase L is generally protective against viral infections, abnormal RNase L expression and activity have been associated with a number of diseases. Here, we show that curcumin, a natural plant-derived anti-inflammatory active principle, inhibits RNase L activity; hence, it may be exploited for therapeutic interventions in case of pathological situations associated with excess activation of RNase L.
authors.affiliation: Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Ankush
authors.initials: A
authors.lastname: Gupta
authors.affiliation: Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Pramod C
authors.initials: PC
authors.lastname: Rath
mesh_terms: D000975:Antioxidants / Q000008:administration & dosage; D018360:Crystallography, X-Ray; D003474:Curcumin / Q000008:administration & dosage* / Q000737:chemistry; D004722:Endoribonucleases / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000737:chemistry; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects*; D062105:Molecular Docking Simulation; D011487:Protein Conformation / Q000187:drug effects; D012330:RNA, Double-Stranded / Q000235:genetics
chemical_list: D000975:Antioxidants; D012330:RNA, Double-Stranded; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease; D003474:Curcumin