title: Identification of DNA aptamers toward epithelial cell adhesion molecule via cell-SELEX.
abstract: The epithelial cell adhesion molecule (EpCAM, also known as CD326) is a transmembrane glycoprotein that is specifically detected in most adenocarcinomas and cancer stem cells. In this study, we performed a Cell systematic evolution of ligands by exponential enrichment (SELEX) experiment to isolate the aptamers against EpCAM. After seven round of Cell SELEX, we identified several aptamer candidates. Among the selected aptamers, EP166 specifically binds to cells expressing EpCAM with an equilibrium dissociation constant (Kd) in a micromolar range. On the other hand, it did not bind to negative control cells. Moreover, EP166 binds to J1ES cells, a mouse embryonic stem cell line. Therefore, the isolated aptamers against EpCAM could be used as a stem cell marker or in other applications in both stem cell and cancer studies.
authors.affiliation: Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea ; Department of Functional Genomics, University of Science and Technology (UST) of Korea, Daejeon 305-806, Korea.
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Kim
authors.affiliation: Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea.
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea ; Department of Functional Genomics, University of Science and Technology (UST) of Korea, Daejeon 305-806, Korea.
authors.forename: Sang Kyung
authors.initials: SK
authors.lastname: Byun
authors.affiliation: Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea ; Department of Functional Genomics, University of Science and Technology (UST) of Korea, Daejeon 305-806, Korea.
authors.forename: Dasom
authors.initials: D
authors.lastname: Lee
authors.affiliation: Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea.
authors.forename: Kyoung-Jin
authors.initials: KJ
authors.lastname: Oh
authors.affiliation: Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea.
authors.forename: Won Kon
authors.initials: WK
authors.lastname: Kim
authors.affiliation: Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea ; Department of Functional Genomics, University of Science and Technology (UST) of Korea, Daejeon 305-806, Korea.
authors.forename: Baek Soo
authors.initials: BS
authors.lastname: Han
authors.affiliation: Medical Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea.
authors.forename: Seung-Wook
authors.initials: SW
authors.lastname: Chi
authors.affiliation: Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea ; Department of Functional Genomics, University of Science and Technology (UST) of Korea, Daejeon 305-806, Korea.
authors.forename: Sang Chul
authors.initials: SC
authors.lastname: Lee
authors.affiliation: Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea ; Department of Functional Genomics, University of Science and Technology (UST) of Korea, Daejeon 305-806, Korea.
authors.forename: Kwang-Hee
authors.initials: KH
authors.lastname: Bae
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D052157:Aptamers, Nucleotide / Q000302:isolation & purification / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D053595:Embryonic Stem Cells / Q000502:physiology*; D000071858:Epithelial Cell Adhesion Molecule; D056945:Hep G2 Cells; D006801:Humans; D051379:Mice; D011485:Protein Binding; D052156:SELEX Aptamer Technique / Q000379:methods*
chemical_list: D000951:Antigens, Neoplasm; D052157:Aptamers, Nucleotide; D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule
keywords: EpCAM; SELEX; aptamer; stem cell marker