title: Red-mediated recombineering of Salmonella enterica genomes.
abstract: The mutagenesis of enterobacterial genomes using phage λ Red recombinase functions is a rapid and versatile experimental tool. In addition to the rapid generation of deletions in the genome of Salmonella enterica, variations of the method allow site-directed mutagenesis, generation of reporter fusions, generation of chimeric genes, or transplantation of regulatory elements directly in the chromosome. We describe the application of these approaches with focus on practical aspects and critical steps.
authors.affiliation: Abteilung Mikrobiologie, Fachbereich Biologie/Chemie, Universität Osnabrück, Barbarastr. 11, 49076, Osnabrück, Germany.
authors.forename: Frederik
authors.initials: F
authors.lastname: Czarniak
authors.forename: Michael
authors.initials: M
authors.lastname: Hensel
mesh_terms: D010582:Bacteriophage lambda / Q000201:enzymology*; D004247:DNA / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D016680:Genome, Bacterial / Q000235:genetics*; D059765:Homologous Recombination; D016296:Mutagenesis; D045522:Recombinases / Q000378:metabolism*; D019779:Salmonella enterica / Q000235:genetics*
chemical_list: D045522:Recombinases; D004247:DNA