title: Chimeric NP non coding regions between type A and C influenza viruses reveal their role in translation regulation.
abstract: Exchange of the non coding regions of the NP segment between type A and C influenza viruses was used to demonstrate the importance not only of the proximal panhandle, but also of the initial distal panhandle strength in type specificity. Both elements were found to be compulsory to rescue infectious virus by reverse genetics systems. Interestingly, in type A influenza virus infectious context, the length of the NP segment 5' NC region once transcribed into mRNA was found to impact its translation, and the level of produced NP protein consequently affected the level of viral genome replication.
authors.affiliation: Unité de Génétique Moléculaire des Virus à ARN, Institut Pasteur, Paris, France; Unité Mixte de Recherche 3569, Centre National de la Recherche Scientifique, Paris, France.
authors.forename: Bernadette
authors.initials: B
authors.lastname: Crescenzo-Chaigne
authors.affiliation: Unité de Génétique Moléculaire des Virus à ARN, Institut Pasteur, Paris, France; Unité Mixte de Recherche 3569, Centre National de la Recherche Scientifique, Paris, France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Barbezange
authors.affiliation: Unité de Génétique Moléculaire des Virus à ARN, Institut Pasteur, Paris, France; Unité Mixte de Recherche 3569, Centre National de la Recherche Scientifique, Paris, France.
authors.forename: Vianney
authors.initials: V
authors.lastname: Frigard
authors.affiliation: Unité de Génétique Moléculaire des Virus à ARN, Institut Pasteur, Paris, France; Unité Mixte de Recherche 3569, Centre National de la Recherche Scientifique, Paris, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Poulain
authors.affiliation: Unité de Génétique Moléculaire des Virus à ARN, Institut Pasteur, Paris, France; Unité Mixte de Recherche 3569, Centre National de la Recherche Scientifique, Paris, France; Université Paris Diderot Sorbonne Paris Cité, Paris, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: van der Werf
mesh_terms: D000818:Animals; D001483:Base Sequence; D004285:Dogs; D015967:Gene Expression Regulation, Viral; D009980:Influenza A virus / Q000235:genetics* / Q000378:metabolism; D009982:Gammainfluenzavirus / Q000235:genetics* / Q000378:metabolism; D061985:Madin Darby Canine Kidney Cells; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D050506:Mutant Chimeric Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019590:Nucleocapsid Proteins; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D059386:Reverse Genetics; D014158:Transcription, Genetic; D014758:Viral Core Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D050506:Mutant Chimeric Proteins; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins