title: Curative effect of Amorphophallus campanulatus (Roxb.) Blume. tuber on N-nitrosodiethylamine- induced hepatocellular carcinoma in rats.
abstract: In this study, we investigated the curative effect of Amorphophallus campanulatus tuber methanolic extract (ACME) on N-nitrosodiethylamine (NDEA)-induced hepatocellular carcinoma (HCC) in experimental rats. All of the rats except those in the normal control group received 0.02% NDEA orally (2 mL, 5 days/week) for the first 20 weeks of the experiment. In different treatment groups, after 20 weeks of NDEA challenge, rats were supplemented with ACME (125 and 250 mg/kg body weight, orally) for the following 28 days. In addition, a standard drug control group was supplemented with silymarin (100 mg/kg bw, orally), a known tumorsuppressive agent against HCC. Administration of ACME significantly inhibited the NDEA-induced increase of hepatic nodule incidence, nodule multiplicity, and serum biochemical indices, and improved the hepatocellular architecture in a dose-dependent manner. The biochemical analysis of hepatic tissues further demonstrated that ACME counteracts NDEA-induced oxidative stress through the restoration of antioxidant enzymes. NDEAadministered rats also showed amplified expression of proliferating cell nuclear antigen in the liver, and decreased expression of this proliferative marker was clearly observed upon the supplementation of ACME. Notably, 250 mg/kg bw ACME supplementation showed better results than the other treatment regimens; this result might be associated with the enhancement of antioxidant activity and inhibition of hepatic cell proliferation.
authors.affiliation: Biochemistry and Pharmacognosy Research Laboratory, School of Biosciences, Mahatma Gandhi University, P.D. Hills. P.O, Kottayam, Kerala, India.
authors.forename: Puthuparampil Nazarudeen
authors.initials: PN
authors.lastname: Ansil
authors.affiliation: Biochemistry and Pharmacognosy Research Laboratory, School of Biosciences, Mahatma Gandhi University, P.D. Hills. P.O, Kottayam, Kerala, India.
authors.forename: Anand
authors.initials: A
authors.lastname: Nitha
authors.affiliation: Biochemistry and Pharmacognosy Research Laboratory, School of Biosciences, Mahatma Gandhi University, P.D. Hills. P.O, Kottayam, Kerala, India.
authors.forename: Santhibhavan Prabhakaran
authors.initials: SP
authors.lastname: Prabha
authors.affiliation: Biochemistry and Pharmacognosy Research Laboratory, School of Biosciences, Mahatma Gandhi University, P.D. Hills. P.O, Kottayam, Kerala, India.
authors.forename: Mukalel Sankunni
authors.initials: MS
authors.lastname: Latha
mesh_terms: D031056:Amorphophallus / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced*; D049109:Cell Proliferation / Q000187:drug effects; D004052:Diethylnitrosamine / Q000633:toxicity*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010088:Oxidoreductases / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D035281:Plant Tubers / Q000737:chemistry; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; D018809:Proliferating Cell Nuclear Antigen; D004052:Diethylnitrosamine; D010088:Oxidoreductases