title: Relationship between the Apolipoprotein AI, B gene polymorphism and the risk of non-traumatic osteonecrosis.
abstract: BACKGROUND
  Previous studies suggested that Apolipoprotein AI (ApoAI) and apolipoprotein B (ApoB) gene polymorphisms may result in lipid metabolism disorders. Genetic polymorphisms in these genes may be associated with the occurrence of osteonecrosis.
  
  
  METHODS
  We designed a case-control study including 429 patients of osteonecrosis and 368 age- and sex-matched control subjects. Polymerase chain reaction was used to amplify the DNA fragments in promoter -75 G > A of ApoAI gene and EcoR I, Xba I and 3'-VNTR of ApoB gene in osteonecrosis patients and healthy controls. We utilized polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to genotype these four single nucleotide polymorphisms (SNPs).
  
  
  RESULTS
  For -75 G > A polymorphism of ApoAI, AA genotype frequency (0.501) was significantly higher in patients with osteonecrosis than that in control (0.462) subjects (P <0.001), GA genotype frequency (0.170) was significantly lower than that in the control (0.310) group (P <0.0001). In osteonecrosis patients, the odds ratio (OR) of A allele was 3.932 (95% CI: 3.0847 ~ 5.0123), which suggested that subjects carrying A allele of promoter region -75 G > A of ApoAI gene had higher susceptibility to osteonecrosis than G allele carriers. The genotype and allele frequency distributions showed no significant difference in EcoR I, Xba Iand 3'-VNTR loci of ApoB gene between the osteonecrosis group and control group.
  
  
  CONCLUSIONS
  Our study suggested that ApoAI gene -75G > A polymorphism may be associated with susceptibility to osteonecrosis in Chinese population. However, our results need further investigation with large sample size and various populations.
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Yin
authors.forename: Zhao
authors.initials: Z
authors.lastname: Liu
authors.forename: Shi-Chang
authors.initials: SC
authors.lastname: Zhao
authors.forename: Yan-Jie
authors.initials: YJ
authors.lastname: Guo
authors.affiliation: Department of Orthopedic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, No,600 Yishan Road, Shanghai 200233, China. zcq791306@163.com.
authors.forename: Zhong-Tang
authors.initials: ZT
authors.lastname: Liu
mesh_terms: D000328:Adult; D016632:Apolipoprotein A-I / Q000235:genetics*; D053299:Apolipoprotein B-100 / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats; D010020:Osteonecrosis / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: C505693:APOA1 protein, human; C569471:APOB protein, human; D016632:Apolipoprotein A-I; D053299:Apolipoprotein B-100