title: Heterotrimeric G proteins control stem cell proliferation through CLAVATA signaling in Arabidopsis.
abstract: Cell-to-cell communication is a fundamental mechanism for coordinating developmental and physiological events in multicellular organisms. Heterotrimeric G proteins are key molecules that transmit extracellular signals; similarly, CLAVATA signaling is a crucial regulator in plant development. Here, we show that Arabidopsis thaliana Gβ mutants exhibit an enlarged stem cell region, which is similar to that of clavata mutants. Our genetic and cell biological analyses suggest that the G protein beta-subunit1 AGB1 and RPK2, one of the major CLV3 peptide hormone receptors, work synergistically in stem cell homeostasis through their physical interactions. We propose that AGB1 and RPK2 compose a signaling module to facilitate meristem development.
authors.affiliation: Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ishida
authors.affiliation: Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Tabata
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan Department of Biology and Institute for Genome Science and Policy Center for Systems Biology, Duke University, Durham, NC, USA.
authors.forename: Masashi
authors.initials: M
authors.lastname: Yamada
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Aida
authors.affiliation: Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Mitsumasu
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Fujiwara
authors.affiliation: Functional Genomics Facility, National Institute for Basic Biology, Okazaki, Japan.
authors.forename: Katsushi
authors.initials: K
authors.lastname: Yamaguchi
authors.affiliation: Functional Genomics Facility, National Institute for Basic Biology, Okazaki, Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Shigenobu
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Higuchi
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsuji
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan.
authors.forename: Ko
authors.initials: K
authors.lastname: Shimamoto
authors.affiliation: Division of Evolutionary Biology, National Institute for Basic Biology, Okazaki, Japan School of Life Science, The Graduate University for Advanced Studies, Okazaki, Japan.
authors.forename: Mitsuyasu
authors.initials: M
authors.lastname: Hasebe
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
authors.forename: Hiroo
authors.initials: H
authors.lastname: Fukuda
authors.affiliation: Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan sawa@kumamoto-u.ac.jp.
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Sawa
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D058506:Cambium / Q000378:metabolism* / Q000502:physiology; D049109:Cell Proliferation; D044387:GTP-Binding Protein beta Subunits / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: C089590:AGB1 protein, Arabidopsis; C118167:AT2G27250 protein, Arabidopsis; D029681:Arabidopsis Proteins; D044387:GTP-Binding Protein beta Subunits; D017346:Protein Serine-Threonine Kinases; C523449:receptor-like protein kinase 2, Arabidopsis
keywords: Arabidopsis thaliana; RECEPTOR‐LIKE PROTEIN KINASE 2; heterotrimeric G protein; peptide hormone; stem cell homeostasis