title: Heteroglycan of an edible mushroom Entoloma lividoalbum: structural characterization and study of its protective role for human lymphocytes.
abstract: A water soluble heteroglycan (PS-II) of an average molecular weight ∼5.2×10(4)Da was isolated from the alkaline extract of an edible mushroom Entoloma lividoalbum (Kühner & Romagn) Kubička. Structural characterization of PS-II was carried out using sugar and methylation analysis, periodate oxidation study, and 1D/2D NMR experiments. Sugar analysis indicated the presence of glucose, mannose, galactose, and fucose in a molar ratio of nearly 5:1:2:1. The repeating unit of the PS-II had a backbone consisting of two (1→3)-β-d-glucopyranosyl, one (1→6)-β-d-glucopyranosyl, one (1→2)-α-L-fucopyranosyl, one (1→6)-α-d-glucopyranosyl, and two (1→6)-α-d-galactopyranosyl residues, out of which one (1→3)-β-d-glucopyranosyl residue was branched at O-6 position with terminal β-d-glucopyranosyl residue and one (1→6)-α-d-galactopyranosyl residue was branched at O-2 position with terminal β-d-mannopyranosyl residue. PS-II showed ameliorative activities at different concentrations (50, 100, 200, 400μg/ml) and maintained the redox balance as well as reduced the lipid peroxidation to protect the cell destruction.
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Maity
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Ashis K
authors.initials: AK
authors.lastname: Nandi
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Ipsita K
authors.initials: IK
authors.lastname: Sen
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Manabendra
authors.initials: M
authors.lastname: Pattanayak
authors.affiliation: Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Sourav
authors.initials: S
authors.lastname: Chattopadhyay
authors.affiliation: Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Sandeep Kumar
authors.initials: SK
authors.lastname: Dash
authors.affiliation: Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Somenath
authors.initials: S
authors.lastname: Roy
authors.affiliation: Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, West Bengal, India.
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Acharya
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India. Electronic address: sirajul_1999@yahoo.com.
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000363:Agaricales; D002478:Cells, Cultured; D019610:Cytoprotection / Q000187:drug effects* / Q000502:physiology; D048690:Fruiting Bodies, Fungal / Q000737:chemistry / Q000302:isolation & purification; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008214:Lymphocytes / Q000187:drug effects* / Q000502:physiology
chemical_list: D062610:Fungal Polysaccharides
keywords: Biological activities; Edible mushrooms; Entoloma lividoalbum (Kühner & Romagn) Kubička; Heteroglycan; NMR studies