title: Complete genome sequence of three tomato ringspot virus isolates: evidence for reassortment and recombination.
abstract: The genome sequence of tomato ringspot virus (ToRSV, a subgroup C nepovirus) is currently available for one raspberry isolate. In this study, we describe the complete genome sequence of three additional isolates from raspberry (Rasp1-2014), grapevine (GYV-2014) and prunus (13C280). The degree of nucleotide sequence identity shared between RNA1 and RNA2 in the 5'-terminal 900 nucleotides and 3' untranslated region varied from 98-99 % (13C280, GYV-2014) to 80 % (Rasp1-2014). Phylogenetic studies revealed distinct origins for Rasp1-2014 RNA1 and RNA2, suggesting reassortment. Two recombination events were also identified in the 3' UTR and 5'-terminal region of RNA1.
authors.affiliation: Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, 4200 Highway 97, PO Box 5000, Summerland, BC, V0H 1Z0, Canada.
authors.forename: Melanie
authors.initials: M
authors.lastname: Walker
authors.forename: Joan
authors.initials: J
authors.lastname: Chisholm
authors.forename: Ting
authors.initials: T
authors.lastname: Wei
authors.forename: Basudev
authors.initials: B
authors.lastname: Ghoshal
authors.forename: Hanna
authors.initials: H
authors.lastname: Saeed
authors.forename: Michael
authors.initials: M
authors.lastname: Rott
authors.forename: Hélène
authors.initials: H
authors.lastname: Sanfaçon
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D016679:Genome, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D017860:Nepovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D027861:Prunus / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D011995:Recombination, Genetic; D065856:Rubus / Q000821:virology*; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics; D027843:Vitis / Q000821:virology*
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D012367:RNA, Viral; D014764:Viral Proteins