title: Stable small quantum dots for synaptic receptor tracking on live neurons.
abstract: We developed a coating method to produce functionalized small quantum dots (sQDs), about 9 nm in diameter, that were stable for over a month. We made sQDs in four emission wavelengths, from 527 to 655 nm and with different functional groups. AMPA receptors on live neurons were labeled with sQDs and postsynaptic density proteins were visualized with super-resolution microscopy. Their diffusion behavior indicates that sQDs access the synaptic clefts significantly more often than commercial QDs.
authors.affiliation: Department of Physics and Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, 1110 W Green St., Urbana, IL 61801 (USA).
authors.forename: En
authors.initials: E
authors.lastname: Cai
authors.forename: Pinghua
authors.initials: P
authors.lastname: Ge
authors.forename: Sang Hak
authors.initials: SH
authors.lastname: Lee
authors.forename: Okunola
authors.initials: O
authors.lastname: Jeyifous
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Liu
authors.forename: Katie M
authors.initials: KM
authors.lastname: Wilson
authors.forename: Sung Jun
authors.initials: SJ
authors.lastname: Lim
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Baird
authors.forename: John E
authors.initials: JE
authors.lastname: Stone
authors.forename: Kwan Young
authors.initials: KY
authors.lastname: Lee
authors.forename: Michael W
authors.initials: MW
authors.lastname: Davidson
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Chung
authors.forename: Klaus
authors.initials: K
authors.lastname: Schulten
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Smith
authors.forename: William N
authors.initials: WN
authors.lastname: Green
authors.forename: Paul R
authors.initials: PR
authors.lastname: Selvin
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000032:analysis*; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000166:cytology*; D061848:Optical Imaging; D045663:Quantum Dots / Q000032:analysis*; D051381:Rats; D018091:Receptors, AMPA / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D018091:Receptors, AMPA
keywords: AMPA receptors; fluorescent probes; quantum dots; single-molecule studies