title: Proteomic study of sporadic inclusion body myositis.
abstract: BACKGROUND
  Sporadic inclusion body myositis (s-IBM) is the most commonly occurring acquired inflammatory myopathy in elderly people (>45 years); however, pathogenic mechanisms are poorly understood and diagnostic tools are limited. In view of this, new therapeutic and diagnostic molecular markers for s-IBM need to be identified.
  
  
  METHODS
  In this study, the proteomes from three s-IBM cases were compared with those from three cases of neurogenic muscular atrophy (control). Proteins were separated by 2-dimensional polyacrylamide gel electrophoresis and profiled by mass spectrometric sequencing and subsequently validated by western blot.
  
  
  RESULTS
  Differential expression was noted in 29 proteins (16 upregulated and 13 downregulated) in s-IBM compared with the control group. Functions of these proteins include oxidative stress response, regulation of apoptosis, signal transduction, and cytoskeleton. Expression of both amyloid precursor protein (APP) and αB-crystallin was increased in s-IBM cases.
  
  
  CONCLUSIONS
  Our study reveals a unique pattern of protein expression in s-IBM, which should be further investigated in a wider cohort of IBM patients to fully realize the potential diagnostic or therapeutic benefits.
authors.affiliation: Department of Geriatric Neurology, Chinese PLA General Hospital, Beijing, 100853 China.
authors.forename: Ke
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Neurology, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853 China.
authors.forename: Chuanqiang
authors.initials: C
authors.lastname: Pu
authors.affiliation: Department of Neurology, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853 China.
authors.forename: Xusheng
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Neurology, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853 China.
authors.forename: Jiexiao
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Neurology, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853 China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Department of Neurology, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853 China.
authors.forename: Xianghui
authors.initials: X
authors.lastname: Lu
keywords: APP; Neurogenic muscular atrophy; Sporadic inclusion body myositis; αB-crystallin