title: [The cytokine homeostasis was regulated by over-expression of Sirt1 in collagen-induced arthritis mice].
abstract: OBJECTIVE
  To investigate the effects of over-expression of Sirt1 on cytokines in the collagen type 2-induced arthritis (CIA) mice.
  
  
  METHODS
  The female DBA/1 mouse CIA model was established by subcutaneous injection of collagen type 2. Recombinant adenovirus carrying Sirt1 gene was infected into CIA mice through tail vein injection. The effects of over-expression of Sirt1 on CIA mice were assessed by measuring arthritis index. The serum levels of cytokines including interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), IL-17, IL-4 and IL-10 in CIA mice were examined by ELISA. The mRNA levels of Sirt1, matrix metalloproteinase-13 (MMP-13) and tissue inhibitor of metalloproteinase-1 (TIMP-1) were detected by real-time quantitative PCR (qRT-PCR). The protein levels of NF-κB subunit p65 and acetylated p65 were determined by Western blotting.
  
  
  RESULTS
  Compared with control groups, over-expression of Sirt1 significantly ameliorated the symptoms of arthritis in CIA mouse. Furthermore, proinflammatory factors (IL-1β, TNF-α, IL-17) decreased while anti-inflammatory factors (IL-4 and IL-10) increased in CIA mice. The over-expression of Sirt1 significantly down-regulated MMP-13 mRNA level and up-regulated TIMP-1 mRNA level. Additionally, the over-expressed Sirt1 reduced acetylation of p65.
  
  
  CONCLUSIONS
  Over-expression of Sirt1 may regulate the balance of cytokines in CIA mice.
authors.affiliation: Department of Orthopedics and Traumatology, First Affiliated Hospital, Henan University of Chinese Medicine, Zhengzhou 450000, China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Orthopedics and Traumatology, First Affiliated Hospital, Henan University of Chinese Medicine, Zhengzhou 450000, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Orthopedics and Traumatology, First Affiliated Hospital, Henan University of Chinese Medicine, Zhengzhou 450000, China.
authors.forename: Yiqiang
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Orthopedics and Traumatology, First Affiliated Hospital, Henan University of Chinese Medicine, Zhengzhou 450000, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics and Traumatology, First Affiliated Hospital, Henan University of Chinese Medicine, Zhengzhou 450000, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000107:Acetylation; D000818:Animals; D001169:Arthritis, Experimental / Q000097:blood* / Q000235:genetics*; D015153:Blotting, Western; D016207:Cytokines / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015870:Gene Expression; D057809:HEK293 Cells; D006706:Homeostasis; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D020381:Interleukin-17 / Q000097:blood; D053583:Interleukin-1beta / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D053509:Matrix Metalloproteinase 13 / Q000235:genetics; D008811:Mice, Inbred DBA; D020133:Reverse Transcriptase Polymerase Chain Reaction; D056564:Sirtuin 1 / Q000235:genetics*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics; D051996:Transcription Factor RelA / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D016207:Cytokines; D020381:Interleukin-17; D053583:Interleukin-1beta; D019715:Tissue Inhibitor of Metalloproteinase-1; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D015847:Interleukin-4; D053509:Matrix Metalloproteinase 13; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1