title: Horseradish peroxidase-encapsulated chitosan nanoparticles for enzyme-prodrug cancer therapy.
abstract: Among various enzyme-based therapies, enzyme-prodrug therapy (EPT) promises minimized side effects in that it activates non-toxic prodrugs locally where the enzymes are placed. The success of such an approach requires high enzyme stability against both structural denaturation and potential immunogenicity. This work examines the efficiency of nanoparticles for enzyme protection in EPT applications. Specifically, horseradish peroxidase (HRP)-encapsulated chitosan nanoparticles (HRP-CSNP) were constructed and examined with respect to stability enhancement. HRP-CSNP retained enzyme activity and had improved stability at 37 °C in the presence of a denaturant, urea. The nanoparticles effectively bound to the surface of human breast cancer cell Bcap37 and led to over 80 % cell death when applied with a prodrug indole-3-acetic acid.
authors.affiliation: Biomedical Nanotechnology Center, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China, xdcao@ecust.edu.cn.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Cao
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.forename: Haijun
authors.initials: H
authors.lastname: Yu
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004355:Drug Stability; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006801:Humans; D007210:Indoleacetic Acids / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D053758:Nanoparticles / Q000737:chemistry*; D011355:Prodrugs / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D000091402:Prohibitins
chemical_list: D000970:Antineoplastic Agents; D007210:Indoleacetic Acids; C000717331:PHB2 protein, human; D011355:Prodrugs; D000091402:Prohibitins; C030737:indoleacetic acid; D048271:Chitosan; D006735:Horseradish Peroxidase