title: Using correlative light and electron microscopy to study zebrafish vascular morphogenesis.
abstract: Live imaging is extremely useful to characterize the dynamics of cellular events in vivo, yet it is limited in terms of spatial resolution. Correlative light and electron microscopy (CLEM) allows combining live confocal microscopy with electron microscopy (EM) for the characterization of biological samples at high temporal and spatial resolution. Here we describe a protocol allowing extracting endothelial cell ultrastructure after having imaged the same cell in its in vivo context through live confocal imaging during zebrafish embryonic development.
authors.affiliation: The Microenvironmental Niche in Tumorigenesis and Targeted Therapy, Inserm U1109, LabEx Medalis, Fédération de Médecine Translationnelle de Strasbourg (FMTS), 67000, Strasbourg, France.
authors.forename: Jacky G
authors.initials: JG
authors.lastname: Goetz
authors.forename: Fabien
authors.initials: F
authors.lastname: Monduc
authors.forename: Yannick
authors.initials: Y
authors.lastname: Schwab
authors.forename: Julien
authors.initials: J
authors.lastname: Vermot
mesh_terms: D000758:Anesthesia; D000818:Animals; D001808:Blood Vessels / Q000196:embryology* / Q000648:ultrastructure*; D004625:Embryo, Nonmammalian / Q000648:ultrastructure; D005453:Fluorescence; D007091:Image Processing, Computer-Assisted; D007834:Lasers; D008854:Microscopy, Electron / Q000379:methods*; D009024:Morphogenesis; D012117:Resins, Synthetic; D016707:Tissue Fixation; D014054:Tomography; D015027:Zebrafish / Q000196:embryology*
chemical_list: D012117:Resins, Synthetic