title: Evaluation of an established pericardium patch for delivery of mesenchymal stem cells to cardiac tissue.
abstract: The present study has evaluated a commercial pericardial material for its capacity to assist as a natural extracellular matrix (ECM) patch for the delivery and retention of mesenchymal stem cells for cardiac repair. The repair of cardiac tissue with cells delivered by an appropriate bioscaffold is expected to offer a superior, long-lasting treatment strategy. The present material, CardioCel®, is based on acellular pericardium that has been stabilized by treatments, including a low concentration of glutaraldehyde, that eliminate calcification after implantation. In the present study, we have assessed this material using human bone marrow mesenchymal stem cells at various cell densities under standard, static cell culture conditions. The initial seeding densities were monitored to evaluate the extent of cell attachment and cell viability, with subsequent cell proliferation assessed up to 4 weeks using an MTS assay. Cell morphology, infiltration, and spreading were tracked using scanning electron microscopy and phalloidin staining. The efficacy of long-term cell survival was further assessed by examining the extent and type of new tissue formation on seeded scaffolds at 70 days; both type I and type III collagens were present in fibrillar structures on these scaffolds indicating that the seeded stem cells had the capacity to differentiate into collagen-producing cells necessary to repair damaged ECM. These data show that the CardioCel® scaffold is an appropriate substrate for the stem cells and has the potential to both retain seeded stem cells and to act as a template for cell propagation and new tissue formation.
authors.affiliation: CSIRO Manufacturing Flagship, Clayton, 3169, Australia.
authors.forename: Aditya V
authors.initials: AV
authors.lastname: Vashi
authors.forename: Jacinta F
authors.initials: JF
authors.lastname: White
authors.forename: Keith M
authors.initials: KM
authors.lastname: McLean
authors.forename: William M L
authors.initials: WM
authors.lastname: Neethling
authors.forename: David I
authors.initials: DI
authors.lastname: Rhodes
authors.forename: John A M
authors.initials: JA
authors.lastname: Ramshaw
authors.forename: Jerome A
authors.initials: JA
authors.lastname: Werkmeister
mesh_terms: D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D009206:Myocardium / Q000166:cytology*; D010496:Pericardium / Q000166:cytology*
chemical_list: D003094:Collagen
keywords: cell proliferation; collagen; collagen synthesis; mesenchymal stem cells; pericardium; scaffold