title: Ionic liquid pretreatment to increase succinic acid production from lignocellulosic biomass.
abstract: In this study, pinewood and corn stover pretreated with the ionic liquid (IL) 1-allyl-3-methylimidazolium chloride (AmimCl) were used as a feedstock for succinic acid production. Results reveal that 5% (v/v) AmimCl inhibited bacterial growth, whereas 0.01% (v/v) AmimCl inhibited succinic acid production. AmimCl was effective in extracting cellulose from pinewood and in degrading pinewood into a uniform pulp, as revealed by scanning electron microscopy (SEM). The rate of enzymatic hydrolysis of pinewood extract reached 72.16%. The combinations of AmimCl pretreatment with steam explosion or with hot compressed water were effective in treating corn stover, whereas AmimCl treatment alone did not result in a significant improvement. Pinewood extract produced 20.7g/L succinic acid with an average yield of 0.37g per gram of biomass. Workflow calculations indicated pine wood pretreated with IL has a theoretical yield of succinic acid of 57.1%. IL pretreatment led to increase in succinic acid yields.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16, Nanxiaojie, Dongzhimennei, Beijing 100700, China; National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Daojiang
authors.initials: D
authors.lastname: Yan
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16, Nanxiaojie, Dongzhimennei, Beijing 100700, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: jmxing@home.ipe.ac.cn.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Xing
mesh_terms: D000498:Allyl Compounds; D018533:Biomass; D005285:Fermentation; D007093:Imidazoles; D052578:Ionic Liquids; D008031:Lignin / Q000378:metabolism*; D028223:Pinus; D019802:Succinic Acid / Q000378:metabolism*; D014934:Wood; D003313:Zea mays
chemical_list: C544334:1-allyl-3-methylimidazolium; D000498:Allyl Compounds; D007093:Imidazoles; D052578:Ionic Liquids; C036909:lignocellulose; D008031:Lignin; D019802:Succinic Acid
keywords: Fermentation; Inhibition; Ionic liquid; Lignocellulosic biomass; Succinic acid