title: Mitochondrial DNA in placenta: associations with fetal growth and superoxide dismutase activity.
abstract: BACKGROUND
  Prenatal growth restraint is associated with increased oxidative stress--as judged by mitochondrial dysfunction--in pregnancies complicated by preeclampsia or diabetes, but it is uncertain whether this is also the case in uncomplicated pregnancies. We assessed the link between fetal growth restraint and placental mitochondrial dysfunction, as reflected by changes in mitochondrial DNA (mtDNA) content and superoxide dismutase (SOD) activity.
  
  
  METHODS
  After uncomplicated pregnancies, placentas (n = 48) were collected at term delivery of singleton infants who were appropriate for gestational age (AGA) or small for gestational age (SGA) (n = 24 in each subgroup). Placental mtDNA content was assessed by real-time PCR, placental SOD activity by colorimetry, and citrate synthase activity--to determine mitochondrial mass--by the spectrophotometric method.
  
  
  RESULTS
  Placentas of SGA infants had a lower mtDNA content (p = 0.015) and a higher SOD activity (p = 0.001) than those of AGA controls. These differences were maintained after normalization of the mtDNA content by citrate synthase activity. In placentas of SGA infants, there was a negative association between mtDNA content and SOD activity (r = -0.58, p = 0.008).
  
  
  CONCLUSIONS
  Fetal growth restraint is accompanied by adaptive changes in the mitochondrial function of the placenta, also in uncomplicated pregnancies.
authors.affiliation: Hospital Sant Joan de Déu, University of Barcelona, Esplugues, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Díaz
authors.forename: Gemma
authors.initials: G
authors.lastname: Aragonés
authors.forename: David
authors.initials: D
authors.lastname: Sánchez-Infantes
authors.forename: Judit
authors.initials: J
authors.lastname: Bassols
authors.forename: Míriam
authors.initials: M
authors.lastname: Pérez-Cruz
authors.forename: Francis
authors.initials: F
authors.lastname: de Zegher
authors.forename: Abel
authors.initials: A
authors.lastname: Lopez-Bermejo
authors.forename: Lourdes
authors.initials: L
authors.lastname: Ibáñez
mesh_terms: D000328:Adult; D004272:DNA, Mitochondrial / Q000378:metabolism*; D005260:Female; D005317:Fetal Growth Retardation / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D008928:Mitochondria / Q000201:enzymology* / Q000473:pathology; D010920:Placenta / Q000201:enzymology* / Q000473:pathology; D011247:Pregnancy; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D004272:DNA, Mitochondrial; D013482:Superoxide Dismutase