title: The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28.
abstract: The complete nucleotide sequence of Alternaria longipes dsRNA virus 1 (AlRV1), a novel double-stranded RNA (dsRNA) mycovirus, was determined and analyzed. AlRV1-HN28 contains a single dsRNA genome segment 3415 base pairs in length (excluding the 3' poly(A) tail) and was predicted to contain two discontiguous open reading frames (ORFs, ORF A and ORF B). The 5'-proximal ORF A (1182 nt) potentially encodes a protein of 394 amino acids (aa) with a predicted molecular mass of 43 kDa; this protein showed no significant similarities to any other sequences in any of the NCBI protein databases. The 3'-proximal ORF B (1737 nt) encodes a protein of 579 aa with a predicted molecular mass of 65 kDa; this protein sequence shares similarities with the conserved domains of RNA-dependent RNA polymerases of other mycoviruses. Phylogenetic analysis indicated that AlRV1-HN28 was closely related to four other unclassified viruses, which suggests that the AlRV1-HN28 isolated from Alternaria longipes may belong to a new family of dsRNA mycoviruses. This is the first report of the full-length nucleotide sequence of a mycovirus that infects Alternaria longipes.
authors.affiliation: Key Laboratory at Universities of Xinjiang, Uygur Autonomous Region for Oasis, Agricultural Pest Management and Plant Protection Resource Utilization, Shihezi University, Shihezi, 832003, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Lin
authors.forename: Hailong
authors.initials: H
authors.lastname: Zhang
authors.forename: Chengjin
authors.initials: C
authors.lastname: Zhao
authors.forename: Shengxue
authors.initials: S
authors.lastname: Liu
authors.forename: Lihua
authors.initials: L
authors.lastname: Guo
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D000528:Alternaria / Q000821:virology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D016679:Genome, Viral / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D012328:RNA Viruses / Q000235:genetics*; D012330:RNA, Double-Stranded; D012367:RNA, Viral / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017423:Sequence Analysis, RNA; D014764:Viral Proteins / Q000235:genetics; D014713:Viruses, Unclassified / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase