title: Adsorption of protein onto double layer mixed matrix membranes.
abstract: This work proposed a novel approach for protein purification by using double layer mixed matrix membranes (MMMs). The double layer MMMs consisting of an active support and separating layer were prepared by co-casting two polymer solutions onto a glass plate. The active support layer consisted of nano hydroxyapatite (HAP) particles embedded in macroporous polyether sulfone (PES) and the separating layer was particle free PES membrane. The influence of separating layer with different PES content on membrane morphology was studied. The double layer MMMs were further characterized concerning permeability and adsorption capacity. The double layer MMMs showed purification of protein via diffusion as well as adsorption. The bovine serum albumin (BSA) was used as a model protein. The properties and structures of double layer MMMs prepared by immersion phase separation process were characterized by pure water flux, BSA adsorption and scanning electron microscopy (SEM).
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Material Science and Engineering, Donghua University, North People Road 2999, Shanghai 201620, PR China. Electronic address: junfensun@dhu.edu.cn.
authors.forename: Junfen
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Chemistry and Chemical Engineering, Heze University, Daxue Road 2269, Heze, Shandong Province 274015, PR China.
authors.forename: Lishun
authors.initials: L
authors.lastname: Wu
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D017886:Durapatite / Q000737:chemistry; D008567:Membranes, Artificial; D011108:Polymers / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013450:Sulfones / Q000737:chemistry
chemical_list: D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; C022840:polyether sulfone; D012710:Serum Albumin, Bovine; D017886:Durapatite
keywords: Adsorption; Bovine serum albumin (BSA); Double layer mixed matrix membranes (MMMs); Hydroxyapatite (HAP); Polyether sulfone