title: Flow-scanning optical tomography.
abstract: We present a 3D tomography technique for in vivo observation of microscopic samples. The method combines flow in a microfluidic channel, illumination through a slit aperture, and a Fourier lens for simultaneous acquisition of multiple perspective angles in the phase-space domain. The technique is non-invasive and naturally robust to parasitic sample motion. 3D absorption is retrieved using standard back-projection algorithms, here a limited-domain inverse radon transform. Simultaneously, 3D differential phase contrast images are obtained by computational refocusing and comparison of complementary illumination angles. We implement the technique on a modified glass slide which can be mounted directly on existing optical microscopes. We demonstrate both amplitude and phase tomography on live, freely swimming C. elegans nematodes.
authors.affiliation: Princeton University, Department of Electrical Engineering, Olden Street, Princeton, NJ 08544, USA. jasonf@princeton.edu.
authors.forename: Nicolas C
authors.initials: NC
authors.lastname: Pégard
authors.forename: Marton L
authors.initials: ML
authors.lastname: Toth
authors.forename: Monica
authors.initials: M
authors.lastname: Driscoll
authors.forename: Jason W
authors.initials: JW
authors.lastname: Fleischer
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D041622:Tomography, Optical / Q000295:instrumentation* / Q000379:methods