title: Analysis of apoptosis induced by perfluorooctane sulfonates (PFOS) in mouse Leydig cells in vitro.
abstract: To explore the possible mechanism of perfluorooctane sulfonates (PFOS's) reproductive toxicity, mouse Leydig cells cultured in vitro were exposed to a serial concentration of PFOS for four more days of culture. Apoptosis during the process was checked. After 24 h, apoptosis occurred to all of the groups ≥ 50 μg/mL PFOS. After 72 h, 37.5 μg/mL dose also showed apoptosis, and the most apoptosis signals, averagely 18 per well, were observed in 62.5 μg/mL dose group. An increase in ROS (p < 0.05) and a decrease of mitochondrial membrane potential (p < 0.01) was confirmed in those groups with ≥ 12.5 μg/mL dose. ROS levels peaked in 50 μg/mL and 62.5 μg/mL groups, nearly two-folds higher than control. PFOS was also observed to down-regulate the protein expression of Bcl-2 and to up-regulate that of Bax. The apoptosis induced by PFOS in mouse Leydig cells was shown to be related to mitochondrially mediated pathways and to involve oxidative stress.
authors.affiliation: College of Biology and Environmental Engineering, Zhejiang Shuren University , Hangzhou , China .
authors.forename: De-Yong
authors.initials: DY
authors.lastname: Zhang
authors.forename: Xiao-Lu
authors.initials: XL
authors.lastname: Xu
authors.forename: Xiu-Ying
authors.initials: XY
authors.lastname: Shen
authors.forename: Qin
authors.initials: Q
authors.lastname: Ruan
authors.forename: Wen-Lang
authors.initials: WL
authors.lastname: Hu
mesh_terms: D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D005466:Fluorocarbons / Q000633:toxicity*; D007985:Leydig Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D017738:Alkanesulfonic Acids; C494827:Bax protein, mouse; D004785:Environmental Pollutants; D005466:Fluorocarbons; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; C052617:Bcl2 protein, mouse; C076994:perfluorooctane sulfonic acid
keywords: Apoptosis; mitochondria pathway; mouse Leydig cells; oxidative stress; perfluorooctane sulfonates