title: Involvement of ralfuranone production in the virulence of Ralstonia solanacearum OE1-1.
abstract: Ralstonia solanacearum causes a destructive disease called "bacterial wilt" in numerous plant species. Its virulence is controlled by the transcriptional regulator PhcA, the activity of which is, in turn, regulated in a cell-density dependent manner, termed quorum sensing. We herein described the identification and characterization of ralfuranones J-L, new PhcA-regulated secondary metabolites, and the known derivatives, ralfuranones A and B, from R. solanacearum strain OE1-1. Their structures were determined by spectroscopic and chemical methods. These ralfuranones were also detected in vascular exudates from host plants infected with OE1-1. Deletion of ralA, which encodes an enzyme for ralfuranone biosynthesis, reduced the virulence of OE1-1 in tomato plants. Virulence was restored by complementation of the ralA gene. The results suggest that ralfuranones play important roles in the virulence of OE1-1.
authors.affiliation: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531 (Japan). kai@biochem.osakafu-u.ac.jp.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kai
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Ohnishi
authors.forename: Yuka
authors.initials: Y
authors.lastname: Mori
authors.forename: Akinori
authors.initials: A
authors.lastname: Kiba
authors.forename: Kouhei
authors.initials: K
authors.lastname: Ohnishi
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Hikichi
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D018551:Solanum lycopersicum / Q000378:metabolism / Q000382:microbiology; D015394:Molecular Structure; D043368:Ralstonia solanacearum / Q000378:metabolism* / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D015107:4-Butyrolactone
keywords: Ralstonia solanacearum; natural products; quorum sensing; ralfuranones; structure elucidation