title: OCH ameliorates bone marrow failure in mice via downregulation of T-bet expression.
abstract: The aim of this study is to evaluate the immune mechanism of OCH in the treatment of AA (also named bone marrow failure, BMF) induced in mice. OCH at a dose of 400 μg/kg was injected intraperitoneally (I.P.) prior to the induction of BMF. Our study showed that the incidence of BMF was 100% in BMF group and 13% in OCH treatment group. Significant higher level of IL-4 and lower level of IFN-γ were observed in OCH group than that in BMF group (P < 0.05) as well as untreated group over BMF (P < 0.05). However, there was no significant difference between OCH and untreated group. Compared with untreated, the expression level of T-bet in OCH and BMF was all significantly higher. However, T-bet expression level was lower in OCH than in BMF. In addition, OCH treatment increased NKT cell fractions of bone marrow and the colonies of CFU-GM. In conclusion, treatment of OCH prior to the induction of BMF could prevent the incidence of BMF possibly through downregulating T-bet expression leading to the transition of immune response from Th1 to Th2, suggesting OCH might be a new therapeutic approach in the treatment of BMF or AA.
authors.affiliation: Department of Paediatrics, Tongji Hospital, Tongji University, 389 Xincun Road, Shanghai 200065, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Qiao
authors.affiliation: Department of Paediatrics, Tongji Hospital, Tongji University, 389 Xincun Road, Shanghai 200065, China.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Paediatrics, Tongji Hospital, Tongji University, 389 Xincun Road, Shanghai 200065, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: Department of Paediatrics, Tongji Hospital, Tongji University, 389 Xincun Road, Shanghai 200065, China.
authors.forename: Jinqing
authors.initials: J
authors.lastname: Tang
authors.affiliation: Department of Paediatrics, Tongji Hospital, Tongji University, 389 Xincun Road, Shanghai 200065, China.
authors.forename: Yuexia
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Department of Paediatrics, Tongji Hospital, Tongji University, 389 Xincun Road, Shanghai 200065, China.
authors.forename: Fuxing
authors.initials: F
authors.lastname: Li
mesh_terms: D000741:Anemia, Aplastic / Q000097:blood / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D001772:Blood Cell Count; D001855:Bone Marrow Diseases; D000080983:Bone Marrow Failure Disorders; D015536:Down-Regulation / Q000187:drug effects*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006017:Glycolipids / Q000008:administration & dosage / Q000494:pharmacology*; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood / Q000378:metabolism / Q000517:prevention & control*; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D053208:Kaplan-Meier Estimate; D008297:Male; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D020825:T-Box Domain Proteins / Q000378:metabolism*; D013997:Time Factors; D000098815:T-bet Transcription Factor
chemical_list: D006017:Glycolipids; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; C501055:tetracosanoic acid 1-galactopyranosyloxy-3,4-dihydroxydec-2-yl amide; D015847:Interleukin-4; D007371:Interferon-gamma