title: The role of FeS clusters for molybdenum cofactor biosynthesis and molybdoenzymes in bacteria.
abstract: The biosynthesis of the molybdenum cofactor (Moco) has been intensively studied, in addition to its insertion into molybdoenzymes. In particular, a link between the assembly of molybdoenzymes and the biosynthesis of FeS clusters has been identified in the recent years: 1) the synthesis of the first intermediate in Moco biosynthesis requires an FeS-cluster containing protein, 2) the sulfurtransferase for the dithiolene group in Moco is also involved in the synthesis of FeS clusters, thiamin and thiolated tRNAs, 3) the addition of a sulfido-ligand to the molybdenum atom in the active site additionally involves a sulfurtransferase, and 4) most molybdoenzymes in bacteria require FeS clusters as redox active cofactors. In this review we will focus on the biosynthesis of the molybdenum cofactor in bacteria, its modification and insertion into molybdoenzymes, with an emphasis to its link to FeS cluster biosynthesis and sulfur transfer.
authors.affiliation: Department of Biochemistry, Duke University Medical Center, Durham, NC, USA.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yokoyama
authors.affiliation: Department of Molecular Enzymology, Institute of Biochemistry and Biology, University of Potsdam, 14476 Potsdam, Germany. Electronic address: sleim@uni-potsdam.de.
authors.forename: Silke
authors.initials: S
authors.lastname: Leimkühler
mesh_terms: D001419:Bacteria / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D003067:Coenzymes / Q000096:biosynthesis* / Q000737:chemistry; D007506:Iron-Sulfur Proteins / Q000737:chemistry / Q000378:metabolism*; D008667:Metalloproteins / Q000096:biosynthesis* / Q000737:chemistry; D008954:Models, Biological; D008956:Models, Chemical; D015394:Molecular Structure; D008982:Molybdenum / Q000737:chemistry / Q000378:metabolism*; D000090006:Molybdenum Cofactors; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D011621:Pteridines / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D003067:Coenzymes; D007506:Iron-Sulfur Proteins; D008667:Metalloproteins; D008982:Molybdenum; D000090006:Molybdenum Cofactors; D010088:Oxidoreductases; D011621:Pteridines; C022261:molybdopterin
keywords: Molybdenum cofactor; Molybdenum–iron–iron–sulfur cluster; Sulfur transfer; l-Cysteine desulfurase; tRNA