title: A study of experimental autoimmune encephalomyelitis in dogs as a disease model for canine necrotizing encephalitis.
abstract: In the present study, the use of dogs with experimental autoimmune encephalomyelitis (EAE) as a disease model for necrotizing encephalitis (NE) was assessed. Twelve healthy dogs were included in this study. Canine forebrain tissues (8 g), including white and grey matter, were homogenized with 4 mL of phosphate-buffered saline for 5 min in an ice bath. The suspension was emulsified with the same volume of Freund's complete adjuvant containing 1 mg/mL of killed Mycobacterium tuberculosis H37Ra. Under sedation, each dog was injected subcutaneously with canine brain homogenate at four sites: two in the inguinal and two in the axillary regions. A second injection (booster) was administered to all the dogs using the same procedure 7 days after the first injection. Clinical assessment, magnetic resonance imaging, cerebrospinal fluid analyses, necropsies, and histopathological and immunohistochemical examinations were performed for the dogs with EAE. Out of the 12 animals, seven (58%) developed clinically manifest EAE at various times after immunization. Characteristics of canine EAE models were very similar to canine NE, suggesting that canine EAE can be a disease model for NE in dogs.
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Jong-Hyun
authors.initials: JH
authors.lastname: Moon
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Jung
authors.forename: Hee-Chun
authors.initials: HC
authors.lastname: Lee
authors.forename: Joon-Hyeok
authors.initials: JH
authors.lastname: Jeon
authors.forename: Na-Hyun
authors.initials: NH
authors.lastname: Kim
authors.forename: Jung-Hyang
authors.initials: JH
authors.lastname: Sur
authors.forename: Jeongim
authors.initials: J
authors.lastname: Ha
authors.forename: Dong-In
authors.initials: DI
authors.lastname: Jung
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology*; D004195:Disease Models, Animal; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D004660:Encephalitis / Q000276:immunology / Q000662:veterinary*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000662:veterinary*; D005260:Female; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D007114:Immunization / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D008279:Magnetic Resonance Imaging / Q000662:veterinary; D008297:Male; D009336:Necrosis / Q000276:immunology / Q000662:veterinary*
keywords: dog; experimental autoimmune encephalomyelitis; multiple sclerosis; necrotizing encephalitis; necrotizing meningoencephalitis