title: Recent insights into microbial catalases: isolation, production and purification.
abstract: Catalase, an oxidoreductase enzyme, works as a detoxification system inside living cells against reactive oxygen species formed as a by-product of different metabolic reactions. The enzyme is found in a wide range of aerobic and anaerobic organisms. Catalase has also been employed in various analytical and diagnostic methods in the form of biosensors and biomarkers in addition to its other applications in textile, paper, food and pharmaceutical industries. New applications for catalases are constantly emerging thanks to their high turnover rate, distinct evolutionary origin, relatively simple and well-defined reaction mechanisms. The following review provides comprehensive information on isolation, production and purification of catalases with different techniques from various microbial sources along with their types, structure, mechanism of action and applications.
authors.affiliation: Enzyme Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, Punjab, 147002, India. Electronic address: soochb@pbi.ac.in.
authors.forename: Balwinder Singh
authors.initials: BS
authors.lastname: Sooch
authors.affiliation: Enzyme Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, Punjab, 147002, India.
authors.forename: Baljinder Singh
authors.initials: BS
authors.lastname: Kauldhar
authors.affiliation: Fermentation and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala 147002, India; Bioprocessing Laboratory, Centre for Chemistry and Biotechnology (CCB), Deakin University, Geelong Technology Precinct, Waurn Ponds Victoria 3216, Australia. Electronic address: munish.puri@deakin.edu.au.
authors.forename: Munish
authors.initials: M
authors.lastname: Puri
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D001709:Biotechnology / Q000379:methods*; D002374:Catalase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D005656:Fungal Proteins; D011994:Recombinant Proteins; D002374:Catalase
keywords: Catalase peroxidase; Downstream processing; Haem-enzyme; Hydrogen peroxide; Oxido-reductase; Protein structure; Purification