title: SPINDLY, ERECTA, and its ligand STOMAGEN have a role in redox-mediated cortex proliferation in the Arabidopsis root.
abstract: Reactive oxygen species (ROS) are harmful to all living organisms and therefore they must be removed to ensure normal growth and development. ROS are also signaling molecules, but so far little is known about the mechanisms of ROS perception and developmental response in plants. We here report that hydrogen peroxide induces cortex proliferation in the Arabidopsis root and that SPINDLY (SPY), an O-linked glucosamine acetyltransferase, regulates cortex proliferation by maintaining cellular redox homeostasis. We also found that mutation in the leucine-rich receptor kinase ERECTA and its putative peptide ligand STOMAGEN block the effect of hydrogen peroxide on root cortex proliferation. However, ERECTA and STOMAGEN are expressed in the vascular tissue, whereas extra cortex cells are produced from the endodermis, suggesting the involvement of intercellular signaling. SPY appears to act downstream of ERECTA, because the spy mutation still caused cortex proliferation in the erecta mutant background. We therefore have not only gained insight into the mechanism by which SPY regulates root development but also uncovered a novel pathway for ROS signaling in plants. The importance of redox-mediated cortex proliferation as a protective mechanism against oxidative stress is also discussed.
authors.affiliation: Department of Biological Science, Florida State University, Tallahassee, FL 32306-4295, USA hcui@bio.fsu.edu.
authors.forename: Hongchang
authors.initials: H
authors.lastname: Cui
authors.affiliation: Department of Biological Science, Florida State University, Tallahassee, FL 32306-4295, USA.
authors.forename: Danyu
authors.initials: D
authors.lastname: Kong
authors.affiliation: Department of Biological Science, Florida State University, Tallahassee, FL 32306-4295, USA Present address: Biotechnical Group, Institute of Rice Research, Anhui Agricultural Academy of Science, 40#, Nongke South Road, Hefei, Anhui, 230031, China.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Wei
authors.affiliation: Department of Biological Science, Florida State University, Tallahassee, FL 32306-4295, USA.
authors.forename: Yueling
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Howard Hughes Medical Institute, Department of Biology, University of Washington, Seattle, WA 98195-1800, USA.
authors.forename: Keiko U
authors.initials: KU
authors.lastname: Torii
authors.affiliation: Howard Hughes Medical Institute, Department of Biology, University of Washington, Seattle, WA 98195-1800, USA.
authors.forename: Jin Suk
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Biological Science, Florida State University, Tallahassee, FL 32306-4295, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide; D009154:Mutation; D010084:Oxidation-Reduction; D018517:Plant Roots / Q000254:growth & development*; D030821:Plants, Genetically Modified; D017346:Protein Serine-Threonine Kinases; D017382:Reactive Oxygen Species / Q000378:metabolism*; D011956:Receptors, Cell Surface; D012097:Repressor Proteins; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D017382:Reactive Oxygen Species; D011956:Receptors, Cell Surface; D012097:Repressor Proteins; C101465:SPY protein, Arabidopsis; C547254:Stomagen protein, Arabidopsis; D006861:Hydrogen Peroxide; C457763:ER protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases
keywords: Arabidopsis thaliana.; ERECTA; ROS signaling; SPY; STOMAGEN; abiotic stress; cortex proliferation; redox homeostasis