title: Fish stress become visible: a new attempt to use biosensor for real-time monitoring fish stress.
abstract: To avoid fish mortality and improve productivity, the physiological conditions including stress state of the cultured fish must be monitored. As an important indicator of stress, glucose concentrations are monitored using in vitro blood analysis. The physiological processes of fish under environmental conditions are harsher in many ways than those experienced by terrestrial animals. Moreover, the process of anaesthetizing and capturing the fish prior to analysis may produce inaccurate results. To solve these problems, we developed wireless biosensor system to monitor the physiological condition of fish. This system enables artificial stress-free and non-lethal analysis, and allows for reliable real-time monitoring of fish stress. The biosensor comprised Pt-Ir wire as the working electrode and Ag/AgCl paste as the reference electrode. Glucose oxidase was immobilized on the working electrode using glutaraldehyde. We used the eyeball interstitial sclera fluid (EISF) as the in vivo implantation site of the sensor, which component concentration correlates well with that of blood component concentration. In the present study, we investigated stress due to alterations in water chemistry, including dissolved oxygen, pH, and ammonia-nitrogen compounds. Stress perceived from behavioural interactions, including attacking behaviour and visual irritation, was also monitored. Water chemistry alterations induced increases in the glucose concentration (stress) that decreased with removal of the stimulus. For behavioural interactions, stress levels change with avoidance, sensory behaviour and activity. We believe that the proposed biosensor system could be useful for rapid, reliable, and convenient analysis of the fish physiological condition and accurately reflects the stress experienced by fish.
authors.affiliation: Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Haiyun
authors.initials: H
authors.lastname: Wu
authors.affiliation: Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Ayasa
authors.initials: A
authors.lastname: Aoki
authors.affiliation: Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Arimoto
authors.affiliation: Graduate School of Agricultural Science Faculty of Agriculture, Tohoku University, 1-1, Tsutsumi-dori, Amamiya-machi, Aoba-ku, Sendai 981-8555, Japan.
authors.forename: Toshiki
authors.initials: T
authors.lastname: Nakano
authors.affiliation: Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 2-1-6, Etchujima, Koto-ku, Tokyo 135-8533, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ohnuki
authors.affiliation: Hokkaido Industrial Technology Center, 379 Kikyo-cho, Hakodate-shi, Hokkaido 041-0801, Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Murata
authors.affiliation: Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Huifeng
authors.initials: H
authors.lastname: Ren
authors.affiliation: Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato-ku, Tokyo 108-8477, Japan. Electronic address: endo@kaiyodai.ac.jp.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Endo
mesh_terms: D000641:Ammonia / Q000737:chemistry / Q000302:isolation & purification; D000818:Animals; D015374:Biosensing Techniques; D005399:Fishes / Q000097:blood / Q000502:physiology*; D005949:Glucose Oxidase / Q000737:chemistry; D008991:Monitoring, Physiologic; D009584:Nitrogen / Q000737:chemistry / Q000302:isolation & purification; D010100:Oxygen / Q000737:chemistry / Q000302:isolation & purification; D013312:Stress, Physiological; D059015:Wireless Technology
chemical_list: D000641:Ammonia; D005949:Glucose Oxidase; D009584:Nitrogen; D010100:Oxygen
keywords: Actual stress; Biosensor; Fish stress; Physiologic responses; Real-time monitoring