title: Single nucleotide polymorphisms in the mitochondrial displacement loop region predict malignant melanoma outcome: a study in Chinese Han population.
abstract: Accumulation of single nucleotide polymorphisms (SNPs) in the displacement loop (D-loop) of mitochondrial DNA (mtDNA) has been identified to be associated with cancer risk and disease outcome. In this study, we investigated whether the SNPs in mitochondrial D-loop were associated with the outcome of malignant melanoma (MM) in Chinese Han population.
  
  
  
  The D-loop region of mtDNA was sequenced for 75 MM patients recorded in the Fourth Hospital of Hebei Medical University between 2003 and 2009. The 5-year survival curve were calculated with the Kaplan-Meier method and compared by the log-rank test at each SNP site, a multivariate survival analysis was also performed with the Cox proportional hazards method.
  
  
  
  The SNP sites of nucleotides T204C, A235G and T16519C were identified for prediction of post-operational survival by the log-rank test. In an overall multivariate analysis, the T204C and A235G alleles were identified as independent predictors of MM outcome.
  
  
  
  Genetic polymorphisms in the D-loop are independent prognostic markers for patients with MM. Accordingly, the analysis of genetic polymorphisms in the mitochondrial D-loop can help identify patient subgroups at high risk of a poor disease outcome.
authors.affiliation: a Department of Orthopedics , The Forth Hospital of Hebei Medical University , Shijiazhuang , China .
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Li
authors.affiliation: b Department of Neurology , The Second Hospital of Hebei Medical University , Shijiazhuang , China .
authors.forename: Binbin
authors.initials: B
authors.lastname: Deng
authors.affiliation: c Department of Clinical Laboratory , The Forth Hospital of Hebei Medical University , Shijiazhuang , China , and.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: a Department of Orthopedics , The Forth Hospital of Hebei Medical University , Shijiazhuang , China .
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Jia
authors.affiliation: a Department of Orthopedics , The Forth Hospital of Hebei Medical University , Shijiazhuang , China .
authors.forename: Xujian
authors.initials: X
authors.lastname: Liu
authors.affiliation: d Department of Internal Medicine , Shenze County Hospital , Shijiazhuang , China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D044466:Asian People / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005006:Ethnicity / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D054629:Genome, Mitochondrial; D006801:Humans; D053208:Kaplan-Meier Estimate; D008545:Melanoma / Q000235:genetics*; D015999:Multivariate Analysis; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial
keywords: D-loop; SNP; malignant melanoma; mtDNA