title: Decreased concentration of IL-35 in plasma of patients with asthma and COPD.
abstract: BACKGROUND
  IL-35 has been found to be involved in many inflammatory diseases in humans but its role in asthma and chronic obstructive pulmonary disease (COPD) is not clear. The plasma level of IL-35 in patients with asthma and COPD needs to be measured.
  
  
  OBJECTIVE
  The aim of this study was to examine the plasma concentrations of IL-35 in newly diagnosed asthmatic and COPD patients and control subjects and investigate correlations of lung function, age, sex, smoking history with the levels of IL-35 in plasma in these diseases.
  
  
  METHODS
  Blood samples were collected from patients with newly diagnosed asthma (44, 12 males, aged 33.75?8.94), newly diagnosed COPD (36, 36 males, aged 68.03 ± 8.94), and healthy control groups (23, 9 males, aged 30.06 ± 7.50). We determined the IL-35 levels in plasma by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The median and the range of values for IL-35 were 118.55 pg/ml (range 74.43~1767.22 pg/ml) in patients with asthma, 136.09 pg/ml (range 62.54~697.49 pg/ml) in patients with COPD and 275.86 pg/ml (range 26.11~1766.20 pg/ml) in control subjects. The levels of IL-35 in plasma showed a positive correlation with FEV1% and FVC% in asthmatic patients whose plasma IL-35 values were over 150 pg/ml. A positive correlation was also found between plasma IL-35 and FVC% in COPD patients whose plasma IL-35 values were over 150 pg/ml.
  
  
  CONCLUSIONS
  These findings suggest that IL-35 may very probably be involved in the Th2 and Th17 mediated inflammation process of asthma and COPD. Its role in the mechanisms of COPD and asthma in human beings, as well as its therapeutic value in these diseases, need further investigation.
authors.affiliation: Department of Respiratory and Critical Care Medicine, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Yanhan
authors.initials: Y
authors.lastname: Deng
authors.forename: Huilong
authors.initials: H
authors.lastname: Chen
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Wu
authors.forename: Sheng
authors.initials: S
authors.lastname: Cheng
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Xu
authors.forename: Weining
authors.initials: W
authors.lastname: Xiong
authors.forename: Jungang
authors.initials: J
authors.lastname: Xie
mesh_terms: D000293:Adolescent; D000328:Adult; D001249:Asthma / Q000097:blood* / Q000276:immunology / Q000473:pathology; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000276:immunology / Q000473:pathology; D007378:Interleukins / Q000097:blood* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood* / Q000276:immunology / Q000473:pathology; D058504:Th17 Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology
chemical_list: D007378:Interleukins; C532417:interleukin-35, human