title: PERK-KIPK-KCBP signalling negatively regulates root growth in Arabidopsis thaliana.
abstract: The Arabidopsis proline-rich, extensin-like receptor-like kinases (PERKs) are a small group of receptor-like kinases that are thought to act as sensors at the cell wall through their predicted proline-rich extracellular domains. In this study, we focused on the characterization of a subclade of three Arabidopsis predicted PERK genes, PERK8, -9, and -10, for which no functions were known. Yeast two-hybrid interaction studies were conducted with the PERK8,- 9, and -10 cytosolic kinase domains, and two members of the Arabidopsis AGC VIII kinase family were identified as interacting proteins: AGC1-9 and the closely related kinesin-like calmodulin-binding protein (KCBP)-interacting protein kinase (KIPK). As KIPK has been identified previously as an interactor of KCBP, these interactions were also examined further and confirmed in this study. Finally, T-DNA mutants for each gene were screened for altered phenotypes under different conditions, and from these screens, a role for the PERK, KIPK, and KCBP genes in negatively regulating root growth was uncovered.
authors.affiliation: Department of Cell & Systems Biology, University of Toronto, Toronto, Canada M5S 3B2.
authors.forename: Tania V
authors.initials: TV
authors.lastname: Humphrey
authors.affiliation: Department of Cell & Systems Biology, University of Toronto, Toronto, Canada M5S 3B2.
authors.forename: Katrina E
authors.initials: KE
authors.lastname: Haasen
authors.affiliation: Department of Cell & Systems Biology, University of Toronto, Toronto, Canada M5S 3B2.
authors.forename: May Grace
authors.initials: MG
authors.lastname: Aldea-Brydges
authors.affiliation: Department of Cell & Systems Biology, University of Toronto, Toronto, Canada M5S 3B2.
authors.forename: He
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Cell & Systems Biology, University of Toronto, Toronto, Canada M5S 3B2.
authors.forename: Yara
authors.initials: Y
authors.lastname: Zayed
authors.affiliation: Department of Cell & Systems Biology, University of Toronto, Toronto, Canada M5S 3B2.
authors.forename: Emily
authors.initials: E
authors.lastname: Indriolo
authors.affiliation: Department of Cell & Systems Biology, University of Toronto, Toronto, Canada M5S 3B2 d.goring@utoronto.ca.
authors.forename: Daphne R
authors.initials: DR
authors.lastname: Goring
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018517:Plant Roots / Q000254:growth & development* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins
keywords: KCBP-interacting protein kinase; Proline-rich extensin-like receptor-like kinase; kinesin-like calmodulin-binding protein; root growth; signalling; sucrose.