title: Ultrasound-mediated gene and drug delivery using a microbubble-liposome particle system.
abstract: Theranostic agents present a promising clinical approach for cancer detection and treatment. We herein introduce a microbubble and liposome complex (MB-Lipo) developed for ultrasound (US) imaging and activation. The MB-Lipo particles have a hybrid structure consisting of a MB complexed with multiple Lipos. The MB components are used to generate high echo signals in US imaging, while the Lipos serve as a versatile carrier of therapeutic materials. MB-Lipo allows high contrast US imaging of tumor sites. More importantly, the application of high acoustic pressure bursts MBs, which releases therapeutic Lipos and further enhances their intracellular delivery through sonoporation effect. Both imaging and drug release could thus be achieved by a single US modality, enabling in situ treatment guided by real-time imaging. The MB-Lipo system was applied to specifically deliver anti-cancer drug and genes to tumor cells, which showed enhanced therapeutic effect. We also demonstrate the clinical potential of MB-Lipo by imaging and treating tumor in vivo.
authors.affiliation: 1. Department of Radiology, Seoul National University College of Medicine, Seoul 110-799, South Korea; ; 2. Department of Radiology, Seoul National University Bundang Hospital, Seungnam 463-707, South Korea; ; 3. Program in Nano Science and Technology, Department of Transdisciplinary Studies, Seoul National University Graduate School of Convergence Science and Technology, Suwon 443-270, South Korea;
authors.forename: Young Il
authors.initials: YI
authors.lastname: Yoon
authors.affiliation: 4. Department of Applied Bioscience, College of Life Science, CHA University, Pocheon 135-081, South Korea;
authors.forename: Yong-Su
authors.initials: YS
authors.lastname: Kwon
authors.affiliation: 4. Department of Applied Bioscience, College of Life Science, CHA University, Pocheon 135-081, South Korea;
authors.forename: Hee-Sang
authors.initials: HS
authors.lastname: Cho
authors.affiliation: 5. Department of Biomedical Science, College of Life Science, CHA University, Pocheon 135-081, South Korea;
authors.forename: Sun-Hee
authors.initials: SH
authors.lastname: Heo
authors.affiliation: 5. Department of Biomedical Science, College of Life Science, CHA University, Pocheon 135-081, South Korea;
authors.forename: Kyeong Soon
authors.initials: KS
authors.lastname: Park
authors.affiliation: 5. Department of Biomedical Science, College of Life Science, CHA University, Pocheon 135-081, South Korea;
authors.forename: Sang Gyu
authors.initials: SG
authors.lastname: Park
authors.affiliation: 5. Department of Biomedical Science, College of Life Science, CHA University, Pocheon 135-081, South Korea;
authors.forename: Soo-Hong
authors.initials: SH
authors.lastname: Lee
authors.affiliation: 1. Department of Radiology, Seoul National University College of Medicine, Seoul 110-799, South Korea; ; 2. Department of Radiology, Seoul National University Bundang Hospital, Seungnam 463-707, South Korea;
authors.forename: Seung Il
authors.initials: SI
authors.lastname: Hwang
authors.affiliation: 1. Department of Radiology, Seoul National University College of Medicine, Seoul 110-799, South Korea;
authors.forename: Young Il
authors.initials: YI
authors.lastname: Kim
authors.affiliation: 1. Department of Radiology, Seoul National University College of Medicine, Seoul 110-799, South Korea;
authors.forename: Hwan Jun
authors.initials: HJ
authors.lastname: Jae
authors.affiliation: 7. Laboratory animal center, KBIO Osong Medical Innovation Foundation, Osong, Cheongwon, Chungbuk 363-951, South Korea;
authors.forename: Gook-Jun
authors.initials: GJ
authors.lastname: Ahn
authors.affiliation: 8. Department of Gastroenterology, Uijeongbu St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Uijeongbu 480-717, South Korea.
authors.forename: Young-Seok
authors.initials: YS
authors.lastname: Cho
authors.affiliation: 9. Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA.
authors.forename: Hakho
authors.initials: H
authors.lastname: Lee
authors.affiliation: 1. Department of Radiology, Seoul National University College of Medicine, Seoul 110-799, South Korea; ; 2. Department of Radiology, Seoul National University Bundang Hospital, Seungnam 463-707, South Korea; ; 3. Program in Nano Science and Technology, Department of Transdisciplinary Studies, Seoul National University Graduate School of Convergence Science and Technology, Suwon 443-270, South Korea; ; 10. Nanoimaging and Therapy Research Center, Institute of Nanoconvergence, Advanced Institutes of Convergence Technology, Seoul National University.
authors.forename: Hak Jong
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: 4. Department of Applied Bioscience, College of Life Science, CHA University, Pocheon 135-081, South Korea;
authors.forename: Tae-Jong
authors.initials: TJ
authors.lastname: Yoon
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000493:pharmacokinetics / Q000627:therapeutic use; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D018014:Gene Transfer Techniques; D006801:Humans; D008081:Liposomes; D045423:Microbubbles; D009369:Neoplasms / Q000188:drug therapy / Q000473:pathology; D011817:Rabbits; D013016:Sound; D014463:Ultrasonography / Q000379:methods*
chemical_list: D000970:Antineoplastic Agents; D008081:Liposomes
keywords: Drug and gene delivery; Liposome; Microbubble; Theranostics.; Ultrasound imaging