title: Purification, characterization, and antioxidant activities of selenium-containing proteins and polysaccharides in royal sun mushroom, Agaricus brasiliensis (Higher Basidiomycetes).
abstract: The Agaricus brasiliensis proves to be the main source of many minerals, especially selenium (Se). In this study, Se-containing polysaccharides and proteins were isolated, purified, and characterized. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical scavenging activity of Se-containing proteins and polysaccharides were also studied. Selenium in A. brasiliensis is present mostly in organic forms, accounting for 81.57% of the total Se. The organic forms of selenium mainly present in Se proteins account for 73.53%, while 12.23% is in Se polysaccharides. Two Se-containing proteins (AB-SePA-22) and (AB-SePG-22) with Se contents of 4.935 µg/g and 6.083 µg/g were obtained. AB-SePA-22 appeared as four bands with molecular masses of 16.7, 21.7, 26.3, and 33.6 kDa, respectively. The Se content of the three Se-containing polysaccharides, namely AB-SeP-1, AB-SeP-2, and AB-SeP-3, were 1.911, 0.613, and 0.671 µg/g, respectively. AB-SeP-1 (3.1×103 Da) was composed of glucose and galactose in a 7.494:1 molar ratio, whereas AB-SeP-2 (2.1×104 Da and 3.5×104 Da) and AB-SeP-3 (1.1×105 Da) were composed of glucose, galactose, and mannose with molar ratios of 27.01:1.55:1 and 9.805:1:1.22, respectively. Moreover, crude Se polysaccharide and total soluble Se protein had good antioxidant activities on scavenging DPPH and hydroxyl radical, and further research is needed.
authors.affiliation: Schools of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013 Jiangsu, China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Mao
authors.affiliation: Schools of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013 Jiangsu, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Feng
authors.affiliation: Schools of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013 Jiangsu, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Schools of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013 Jiangsu, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Schools of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013 Jiangsu, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.affiliation: Schools of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013 Jiangsu, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Schools of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013 Jiangsu, China.
authors.forename: Yuena
authors.initials: Y
authors.lastname: Ren
authors.affiliation: Schools of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013 Jiangsu, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Schools of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013 Jiangsu, China.
authors.forename: Liuqing
authors.initials: L
authors.lastname: Yang
authors.affiliation: Schools of Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013 Jiangsu, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Wu
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D001713:Biphenyl Compounds / Q000378:metabolism; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D010851:Picrates / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D012643:Selenium / Q000032:analysis*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005656:Fungal Proteins; D010851:Picrates; D011134:Polysaccharides; C004931:1,1-diphenyl-2-picrylhydrazyl; D012643:Selenium