title: Screening and identification of multi-components in Re Du Ning injections using LC/TOF-MS coupled with UV-irradiation.
abstract: A high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry-UV-irradiation (HPLC-QTOF-MS-UV) method was established for rapid separation and structural identification of the constituents in Re Du Ning Injections (RDNI). A total of 20 potentially bioactive compounds including 10 caffeoylquinic acids and 10 iridoid glycosides were identified or tentatively characterized in RDNI by comparing their retention times and MS spectra with those of authentic standards or literature data. In particular, UV-irradiation was employed in the identification of the cis/trans isomers of caffeoylquinic acids. Furthermore, each compound was assigned to the individual raw materials (Artemisia annua L., Lonicera japonica Thunb. or Gardenia jasminoides Ellis) present in RDNI. This is the first time that an HPLC-QTOF-MS-UV analytical method has been used for the identification of caffeoylquinic acids in RDNI.
authors.affiliation: State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, P.R. China School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 103Wenhua Rd., Shenyang 110016, P.R. China.
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, P.R. China Jiangsu Kanon Pharmaceutical Co., Ltd, Lianyungang 222001, P.R. China.
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, 103Wenhua Rd., Shenyang 110016, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.affiliation: State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, P.R. China Jiangsu Kanon Pharmaceutical Co., Ltd, Lianyungang 222001, P.R. China.
authors.forename: Xiaohuan
authors.initials: X
authors.lastname: Fu
authors.affiliation: State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, P.R. China Jiangsu Kanon Pharmaceutical Co., Ltd, Lianyungang 222001, P.R. China.
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, P.R. China Jiangsu Kanon Pharmaceutical Co., Ltd, Lianyungang 222001, P.R. China wzhzh-nj@163.net songsj99@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 103Wenhua Rd., Shenyang 110016, P.R. China wzhzh-nj@163.net songsj99@163.com.
authors.forename: Shaojiang
authors.initials: S
authors.lastname: Song
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000528:radiation effects; D015394:Molecular Structure; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D014466:Ultraviolet Rays
chemical_list: D004365:Drugs, Chinese Herbal