title: Identification of biomarkers for radiation-induced acute intestinal symptoms (RIAISs) in cervical cancer patients by serum protein profiling.
abstract: Radiation-induced acute intestinal symptoms (RIAISs) are the most frequent complication of radiotherapy that causes great pain and limits the treatment efficacy. The aim of this study was to identify serum biomarkers of RIAISs in cervical cancer patients by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). Serum samples were collected from 66 cervical cancer patients prior to pelvic radiotherapy. In our study, RIAISs occurred in 11 patients. An additional 11 patients without RIAISs were selected as controls, whose age, stage, histological type and treatment methods were matched to RIAISs patients. The 22 sera were subsequently analyzed by SELDI-TOF MS, and the resulting protein profiles were evaluated to identify biomarkers using appropriate bioinformatics tools. Comparing the protein profiles of serum samples from the RIAIS group and the control group, it was found that 22 protein peaks were significantly different (P < 0.05), and six of these peaks with mass-to-charge (m/z) ratios of 7514.9, 4603.94, 6887.41, 2769.21, 3839.72 and 4215.7 were successfully identified. A decision tree model of biomarkers was constructed based on three biomarkers (m/z 1270.88, 1503.23 and 7514.90), which separated RIAIS-affected patients from the control group with an accuracy of 81%. This study suggests that serum proteomic analysis by SELDI-TOF MS can identify cervical cancer patients that are susceptible to RIAISs prior to pelvic radiotherapy.
authors.affiliation: Department of Radiotherapy Oncology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, P. R. China.
authors.forename: Yanlan
authors.initials: Y
authors.lastname: Chai
authors.affiliation: Department of Radiotherapy Oncology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, P. R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Radiotherapy Oncology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Radiotherapy Oncology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, P. R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Radiotherapy Oncology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, P. R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Shi
authors.affiliation: Department of Radiotherapy Oncology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, P. R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Su
authors.affiliation: Department of Radiotherapy Oncology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, P. R. China.
authors.forename: Yunyi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Radiotherapy Oncology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, P. R. China Renmin Hospital, Hubei University of Medicine, Hubei 442000, P. R. China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Radiotherapy Oncology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, P. R. China.
authors.forename: Liping
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of Radiotherapy Oncology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, P. R. China liuzmail@163.com.
authors.forename: Zi
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D001681:Biological Assay / Q000379:methods; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D007410:Intestinal Diseases / Q000097:blood* / Q000175:diagnosis / Q000209:etiology*; D008875:Middle Aged; D011832:Radiation Injuries / Q000097:blood* / Q000175:diagnosis / Q000209:etiology*; D020266:Radiotherapy, Conformal / Q000009:adverse effects; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016896:Treatment Outcome; D002583:Uterine Cervical Neoplasms / Q000097:blood / Q000532:radiotherapy*
chemical_list: D015415:Biomarkers; D001798:Blood Proteins
keywords: SELDI-TOF MS; biomarker identification; cervical cancer; protein profiling; radiation-induced acute intestinal symptom