title: Phytochemical analysis of Pfaffia glomerata inflorescences by LC-ESI-MS/MS.
abstract: Pfaffia glomerata contains high levels of β-ecdysone, which has shown a range of beneficial pharmacological effects. The present study demonstrated that inflorescences of P. glomerata contain other important bioactive compounds in addition to β-ecdysone. The identification of compounds from inflorescences using liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) was performed for the first time. The eight compounds identified were β-ecdysone, flavonoid glycosides such as quercetin-3-O-glucoside, kaempferol-3-O-glucoside and kaempferol-3-O-(6-p-coumaroyl)-glucoside, oleanane-type triterpenoid saponins such as ginsenoside Ro and chikusetsusaponin IV, in addition to oleanonic acid and gluconic acid. This study provided information on the phytochemicals contained in P. glomerata inflorescences revealing the potential application of this plant part as raw material for the phytotherapeutic and cosmetic industries.
authors.affiliation: Pharmaceutical Sciences Postgraduate Program, Department of Pharmacy, State University of Maringá, Av. Colombo, 5790, Maringá, Paraná 87020-900, Brazil. daniele.felipe@uol.com.br.
authors.forename: Daniele F
authors.initials: DF
authors.lastname: Felipe
authors.affiliation: Pharmaceutical Sciences Postgraduate Program, Department of Pharmacy, State University of Maringá, Av. Colombo, 5790, Maringá, Paraná 87020-900, Brazil. larazampar@yahoo.com.br.
authors.forename: Lara Z S
authors.initials: LZ
authors.lastname: Brambilla
authors.affiliation: Department of Chemistry, State University of Maringá, Av. Colombo, 5790, Maringá, Paraná 87020-900, Brazil. cporto.silva@gmail.com.
authors.forename: Carla
authors.initials: C
authors.lastname: Porto
authors.affiliation: Department of Chemistry, State University of Maringá, Av. Colombo, 5790, Maringá, Paraná 87020-900, Brazil. ejpilau@uem.br.
authors.forename: Eduardo J
authors.initials: EJ
authors.lastname: Pilau
authors.affiliation: Pharmaceutical Sciences Postgraduate Program, Department of Pharmacy, State University of Maringá, Av. Colombo, 5790, Maringá, Paraná 87020-900, Brazil. dagcortez@uem.br.
authors.forename: Diógenes A G
authors.initials: DA
authors.lastname: Cortez
mesh_terms: D027703:Amaranthaceae / Q000737:chemistry*; D002853:Chromatography, Liquid; D015394:Molecular Structure; D064209:Phytochemicals / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D064209:Phytochemicals