title: Evaluation of fibroblasts adhesion and proliferation on alginate-gelatin crosslinked hydrogel.
abstract: Due to the relatively poor cell-material interaction of alginate hydrogel, alginate-gelatin crosslinked (ADA-GEL) hydrogel was synthesized through covalent crosslinking of alginate di-aldehyde (ADA) with gelatin that supported cell attachment, spreading and proliferation. This study highlights the evaluation of the physico-chemical properties of synthesized ADA-GEL hydrogels of different compositions compared to alginate in the form of films. Moreover, in vitro cell-material interaction on ADA-GEL hydrogels of different compositions compared to alginate was investigated by using normal human dermal fibroblasts. Viability, attachment, spreading and proliferation of fibroblasts were significantly increased on ADA-GEL hydrogels compared to alginate. Moreover, in vitro cytocompatibility of ADA-GEL hydrogels was found to be increased with increasing gelatin content. These findings indicate that ADA-GEL hydrogel is a promising material for the biomedical applications in tissue-engineering and regeneration.
authors.affiliation: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Bapi
authors.initials: B
authors.lastname: Sarker
authors.affiliation: Department of Cardiology and Angiology, University Hospital Erlangen, Erlangen, Germany.
authors.forename: Raminder
authors.initials: R
authors.lastname: Singh
authors.affiliation: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Raquel
authors.initials: R
authors.lastname: Silva
authors.affiliation: Institute of Polymer Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Judith A
authors.initials: JA
authors.lastname: Roether
authors.affiliation: Institute of Polymer Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Kaschta
authors.affiliation: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Detsch
authors.affiliation: Institute of Polymer Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Dirk W
authors.initials: DW
authors.lastname: Schubert
authors.affiliation: Department of Cardiology and Angiology, University Hospital Erlangen, Erlangen, Germany; Cardiovascular Nanomedicine Unit, Section of Experimental Oncology and Nanomedicine, ENT Department, University Hospital, Erlangen, Germany.
authors.forename: Iwona
authors.initials: I
authors.lastname: Cicha
authors.affiliation: Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Aldo R
authors.initials: AR
authors.lastname: Boccaccini
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D061251:Primary Cell Culture; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D020100:Hydrogels; D005780:Gelatin