title: Mycobacterium genotypes in pulmonary tuberculosis infections and their detection by trained African giant pouched rats.
abstract: Tuberculosis (TB) diagnosis in low-income countries is mainly done by microscopy. Hence, little is known about the diversity of Mycobacterium spp. in TB infections. Different genotypes or lineages of Mycobacterium tuberculosis vary in virulence and induce different inflammatory and immune responses. Trained Cricetomys rats show a potential for rapid diagnosis of TB. They detect over 28 % of smear-negative, culture-positive TB. However, it is unknown whether these rats can equally detect sputa from patients infected with different genotypes of M. tuberculosis. A 4-month prospective study on diversity of Mycobacterium spp. was conducted in Dar es Salaam, Tanzania. 252 sputa from 161 subjects were cultured on Lowenstein-Jensen medium and thereafter tested by rats. Mycobacterial isolates were subjected to molecular identification and multispacer sequence typing (MST) to determine species and genotypes. A total of 34 Mycobacterium spp. isolates consisting of 32 M. tuberculosis, 1 M. avium subsp. hominissuis and 1 M. intracellulare were obtained. MST analyses of 26 M. tuberculosis isolates yielded 10 distinct MST genotypes, including 3 new genotypes with two clusters of related patterns not grouped by geographic areas. Genotype MST-67, shared by one-third of M. tuberculosis isolates, was associated with the Mwananyamala clinic. This study shows that diverse M. tuberculosis genotypes (n = 10) occur in Dar es Salaam and trained rats detect 80 % of the genotypes. Sputa with two M. tuberculosis genotypes (20 %), M. avium hominissuis and M. intracellulare were not detected. Therefore, rats detect sputa with different M. tuberculosis genotypes and can be used to detect TB in resource-poor countries.
authors.affiliation: Department of Immunology, Max Planck Institute for Infection Biology, Charitéplatz 1, Campus Charité Mitte, 10117, Berlin, Germany.
authors.forename: Georgies F
authors.initials: GF
authors.lastname: Mgode
authors.forename: Stéphan
authors.initials: S
authors.lastname: Cohen-Bacrie
authors.forename: Marielle
authors.initials: M
authors.lastname: Bedotto
authors.forename: Bart J
authors.initials: BJ
authors.lastname: Weetjens
authors.forename: Christophe
authors.initials: C
authors.lastname: Cox
authors.forename: Maureen
authors.initials: M
authors.lastname: Jubitana
authors.forename: Dian
authors.initials: D
authors.lastname: Kuipers
authors.forename: Robert S
authors.initials: RS
authors.lastname: Machang'u
authors.forename: Rudovick
authors.initials: R
authors.lastname: Kazwala
authors.forename: Sayoki G
authors.initials: SG
authors.lastname: Mfinanga
authors.forename: Stefan H E
authors.initials: SH
authors.lastname: Kaufmann
authors.forename: Michel
authors.initials: M
authors.lastname: Drancourt
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002648:Child; D002675:Child, Preschool; D005260:Female; D005838:Genotype; D062306:Geography, Medical; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D058885:Multilocus Sequence Typing; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D011446:Prospective Studies; D051381:Rats; D013183:Sputum / Q000382:microbiology; D013636:Tanzania; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000382:microbiology*; D055815:Young Adult