title: Effects of intestinal microbiota on the bioavailability of geniposide in rats.
abstract: This study investigated the effects of intestinal microbiota on the metabolism of geniposide by using a rat model treated with a mixture of antibiotics. The plasma concentration of geniposide was determined after oral administration in control and antibiotics-treated rats by using liquid chromatography-tandem mass spectrometry. The maximum plasma concentrations (Cmax) of geniposide in control and antibiotics-treated rats were 0.91 ± 0.26 and 1.01 ± 0.04 μg/mL, respectively, and the area under the curve (AUC) values were 7.34 ± 3.32 and 11.9 ± 2.1 μg·h/mL (p < 0.05), respectively. The levels of geniposide in rat feces were 0.64 and 15.6 mg, respectively, in the control and antibiotics-treated groups. Thus, the systemic exposure of geniposide was greater in the antibiotics-treated rats. This may be due to the antibiotic-induced suppression of the metabolic activities of the intestinal microbiota. These results suggest that the gut microbiota may have an impact on the bioavailability of geniposide.
authors.affiliation: Institute of Pharmaceutical Science and Technology and College of Pharmacy, Hanyang University , Ansan, Gyeonggi-do, Korea.
authors.forename: Ming Ji
authors.initials: MJ
authors.lastname: Jin
authors.forename: In Sook
authors.initials: IS
authors.lastname: Kim
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Hye Hyun
authors.initials: HH
authors.lastname: Yoo
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D007422:Intestines / Q000187:drug effects* / Q000382:microbiology*; D039823:Iridoids / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008297:Male; D064307:Microbiota / Q000187:drug effects; D017207:Rats, Sprague-Dawley
chemical_list: D039823:Iridoids; C007835:geniposide
keywords: bioavailability; geniposide; intestinal microbiota; metabolism