title: Infection of water buffalo in Rio de Janeiro Brazil with Anaplasma marginale strains also reported in cattle.
abstract: Anaplasma marginale is the most prevalent pathogen of cattle in tropical and subtropical regions of the world and causes the disease bovine anaplasmosis. The importance of water buffalo in the world economy is increasing. In addition, while water buffalo may serve as a reservoir host for A. marginale, the susceptibility of this host for A. marginale cattle strains in Brazil has not been reported. The major surface protein 1 alpha (msp1α) gene has been shown to be a stable genetic marker for identification of A. marginale strains. Herein, we analyzed blood samples from 200 water buffalo and identified the A. marginale strains in an endemic area of Rio de Janeiro, Brazil, where ticks were present and water buffalo and cattle co-mingled. Ticks that were feeding on the study buffalo were collected and identified. The prevalence of A. marginale in water buffalo in this study was low (10%). Sequence analysis of the msp1α gene demonstrated the presence of 8 different A. marginale strains. Two A. marginale strains in the water buffalo, (α-β-β-β-Γ) and (α-β-β-Γ), were similar to those reported in cattle from nearby regions. The results of this study suggested that water buffalo in this region are naturally infected with the same strains of A. marginale found in cattle.
authors.affiliation: Departamento de Patologia Veterinária, Universidade Estadual Paulista, Jaboticabal, São Paulo, Brazil.
authors.forename: Jenevaldo B
authors.initials: JB
authors.lastname: Silva
authors.affiliation: SaBio, Instituto de Investigación en Recursos Cinegéticos IREC, CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain; Center for Infection and Immunity of Lille (CIIL), INSERM U1019-CNRS UMR 8204, Université Lille Nord de France, Institut Pasteur de Lille, Lille, France. Electronic address: cabezasalejandrocruz@gmail.com.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Cabezas-Cruz
authors.affiliation: Departamento de Clínica Veterinária, Universidade Federal do Pará, Castanhal, Pará, Brazil.
authors.forename: Adivaldo H
authors.initials: AH
authors.lastname: Fonseca
authors.affiliation: Departamento de Parasitologia, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, Brazil.
authors.forename: José D
authors.initials: JD
authors.lastname: Barbosa
authors.affiliation: SaBio, Instituto de Investigación en Recursos Cinegéticos IREC, CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain; Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA. Electronic address: Jose_delafuente@yahoo.com.
authors.forename: José
authors.initials: J
authors.lastname: de la Fuente
mesh_terms: D000595:Amino Acid Sequence; D042322:Anaplasma marginale / Q000235:genetics / Q000302:isolation & purification*; D000712:Anaplasmosis / Q000453:epidemiology* / Q000382:microbiology; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001938:Brazil / Q000453:epidemiology; D002020:Buffaloes / Q000382:microbiology*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000382:microbiology; D004197:Disease Reservoirs / Q000382:microbiology; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013987:Ticks / Q000382:microbiology*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D005819:Genetic Markers; C449089:major surface protein 1a, Anaplasma
keywords: Anaplasma marginale; Bovine anaplasmosis; Ticks; Water buffalo; msp1α