title: Development of rat embryonic spinal ganglion cells in damaged nerve.
abstract: The development of dissociated cells from rat embryonic spinal ganglion after transplantation to damaged nerve of adult animals was studied using immunohistochemical differentiation markers of neural and glial cells. The cell suspension obtained after dissociation of rat embryonic spinal ganglia (embryonic day 15) was injected into the proximal segment of crushed sciatic nerve. The nerve was damaged by ligation for 40 sec. Progenitor cells were labeled with 5-bromo-2'-deoxyuridine (BrdU) before transplantation. BrdU-immunopositive cells were detected in the nerve trunks of recipients on days 1, 21, and 28 after transplantation. Dissociated cells of rat embryonic spinal ganglion (embryonic day 15) survived for at least 4 weeks after transplantation to the nerve and differentiate into NeuN-immunopositive neurons with morphological properties of sensory neurons and satellite cells containing S100 protein.
authors.affiliation: Laboratory of Functional Morphology of Central and Peripheral Nervous System, Department of General and Special Morphology, Research Institute of Experimental Medicine, North-West Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia, iemmorphol@yandex.ru.
authors.forename: E S
authors.initials: ES
authors.lastname: Petrova
authors.forename: E N
authors.initials: EN
authors.lastname: Isaeva
authors.forename: D E
authors.initials: DE
authors.lastname: Korzhevskii
mesh_terms: D000818:Animals; D005727:Ganglia, Spinal / Q000166:cytology* / Q000196:embryology / Q000293:injuries; D051381:Rats; D017208:Rats, Wistar