title: Garlic supplemented diet attenuates gentamicin nephrotoxicity ın rats.
abstract: OBJECTIVE
  To demonstrate the effect of a 4% pulverized garlic supplemented diet on the nephrotoxicity induced by gentamicin in rats.
  
  
  METHODS
  Twenty four healthy male Wistar rats, weighing between 220 - 260 grams, were divided into three groups. The rats were randomly assigned to either the gentamicin injection without garlic supplementation group (Group I, n = 8), gentamicin injection with garlic supplementation group (Group II, n = 8), and control group (Group III, n = 8). Urine from the rats was collected and the volume (mL), microalbumin (mg/L), creatinine (mg/dL), Na (mmol/L), K (mmol/L), Cl (mmol/L), P (mg/dL), N-acetyl glucosamine (NAG) (U/L) and pH values were measured. Then urea (mg/dL), creatinine (mg/dL), total protein (g/dL) and cystatin (mg/L) values were measured for the blood samples obtained from tail veins.
  
  
  RESULTS
  The median NAG value for the control group (52.050 U/L) was similar to value for Group II (56.400 U/L), which received gentamicin and the garlic diet. However, the median NAG value for Group I (77.030 U/L), which received gentamicin without garlic supplementation, was determined to be statistically significantly higher (p = 0.010) than the value for the control group. In addition, the mean cystatin value for Group II (1.360 U/L) was found to be statistically significantly lower than the value for the Group I (2.240 U/L) (p = 0.015).
  
  
  CONCLUSIONS
  In this study we showed the effect of 4% pulverized garlic supplemented diet for preventing nephrotoxicity induced by gentamicin in rats by using as parameters NAG in urine samples and cystatin C in serum samples.
authors.affiliation: Department of Urology, University of Gaziantep, Gaziantep, Turkey.
authors.forename: Ilker
authors.initials: I
authors.lastname: Seckiner
authors.affiliation: Department of Urology, University of Gaziantep, Gaziantep, Turkey.
authors.forename: Omer
authors.initials: O
authors.lastname: Bayrak
authors.affiliation: Department of Biochemistry, University of Bulent Ecevit, Zonguldak, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Can
authors.affiliation: Department of Biochemistry, University of Bulent Ecevit, Zonguldak, Turkey.
authors.forename: A Gorkem
authors.initials: AG
authors.lastname: Mungan
authors.affiliation: Department of Urology, University of Bulent Ecevit, Zonguldak, Turkey.
authors.forename: N Aydin
authors.initials: NA
authors.lastname: Mungan
mesh_terms: D000117:Acetylglucosamine / Q000652:urine; D000419:Albuminuria; D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D003404:Creatinine / Q000097:blood / Q000652:urine; D055316:Cystatin C / Q000097:blood; D019587:Dietary Supplements; D005737:Garlic; D005839:Gentamicins / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects*; D008297:Male; D011897:Random Allocation; D017208:Rats, Wistar; D012016:Reference Values; D015203:Reproducibility of Results; D016896:Treatment Outcome; D014508:Urea / Q000097:blood; D016482:Urinalysis
chemical_list: D000900:Anti-Bacterial Agents; D055316:Cystatin C; D005839:Gentamicins; D014508:Urea; D003404:Creatinine; D000117:Acetylglucosamine