title: Microbiological characterization of Delftia acidovorans clinical isolates from patients in an intensive care unit in Brazil.
abstract: Delftia acidovorans is an opportunistic agent in several types of infections, both in immunocompromised and immune-competent individuals; its resistance to aminoglycosides and polymyxin, choice drugs for empirical treatment of Gram-negative infections, is remarkable. We report the antimicrobial susceptibility and the genetic relatedness of 24 D. acidovorans strains recovered from tracheal aspirates of 21 adult inpatients hospitalized in an intensive care unit at a Brazilian hospital, from 2012 to 2013. All of the isolates were recovered as pure cultures and in counts above 1,000,000 CFU/mL. None of them were susceptible to polymyxin B, amikacin, gentamicin, or tobramycin; quinolones and trimethoprim-sulfamethoxazole presented varied activities against the isolates, while β-lactam resistance was not detected. Four clusters were verified in pulsed-field gel electrophoresis analysis, and a major pulsotype comprised 10 strains. A possible, but undetermined common source, can be responsible for this strain dissemination, underscoring the need of reinforcing the adherence to disinfection and infection control standard techniques.
authors.affiliation: Instituto Adolfo Lutz, Sao Paulo, Brazil. Electronic address: chcamargo@ial.sp.gov.br.
authors.forename: Carlos Henrique
authors.initials: CH
authors.lastname: Camargo
authors.affiliation: Hospital Amaral Carvalho, Jau, Brazil; Botucatu Medical School, Botucatu, Brazil.
authors.forename: Adriano Martison
authors.initials: AM
authors.lastname: Ferreira
authors.affiliation: Hospital Amaral Carvalho, Jau, Brazil.
authors.forename: Edvaldo
authors.initials: E
authors.lastname: Javaroni
authors.affiliation: Hospital Amaral Carvalho, Jau, Brazil.
authors.forename: Brígida Aparecida Rosa
authors.initials: BA
authors.lastname: Reis
authors.affiliation: Instituto Adolfo Lutz, Sao Paulo, Brazil.
authors.forename: Maria Fernanda Campagnari
authors.initials: MF
authors.lastname: Bueno
authors.affiliation: Instituto Adolfo Lutz, Sao Paulo, Brazil.
authors.forename: Gabriela Rodrigues
authors.initials: GR
authors.lastname: Francisco
authors.affiliation: Instituto Adolfo Lutz, Sao Paulo, Brazil.
authors.forename: Juliana Failde
authors.initials: JF
authors.lastname: Gallo
authors.affiliation: Instituto Adolfo Lutz, Sao Paulo, Brazil.
authors.forename: Doroti de Oliveira
authors.initials: Dde O
authors.lastname: Garcia
mesh_terms: D000583:Amikacin / Q000494:pharmacology; D001938:Brazil; D020605:Delftia acidovorans / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D024881:Drug Resistance, Bacterial / Q000187:drug effects*; D016521:Electrophoresis, Gel, Pulsed-Field; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D007362:Intensive Care Units; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D012336:RNA, Ribosomal, 16S; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000494:pharmacology
chemical_list: D012336:RNA, Ribosomal, 16S; D015662:Trimethoprim, Sulfamethoxazole Drug Combination; D000583:Amikacin
keywords: Cross infection; Delftia acidovorans; Drug resistance; Electrophoresis; Gel; Microbial sensitivity tests; Molecular epidemiology; Pulsed-field