title: Comparative evaluation of two Trichoderma harzianum strains for major secondary metabolite production and antifungal activity.
abstract: This investigation was undertaken to identify the major secondary metabolite, produced by two Trichoderma harzianum strains (T-4 and T-5) with their antifungal activity against phytopathogenic fungi using poison food technique. The ethyl acetate extract was subjected to column chromatography using n-hexane, ethyl acetate and methanol gradually. Chromatographic separation of ethyl acetate extract of T. harzianum (T-4) resulted in the isolation and identification of palmitic acid (1), 1,8-dihydroxy-3-methylanthraquinone (2), 6-pentyl-2H-pyran-2-one (3), 2(5H)-furanone (4), stigmasterol (5) and β-sitosterol (6), while T. harzianum (T-5) gave palmitic acid (1), 1-hydroxy-3-methylanthraquinone (7), δ-decanolactone (8), 6-pentyl-2H-pyran-2-one (3), ergosterol (9), harzianopyridone (10) and 6-methyl-1,3,8-trihydroxyanthraquinone (11) as major metabolites. Among compounds screened for antifungal activity, compound 10 was found to be most active (EC50 35.9-50.2 μg mL(-1)). In conclusion, the present investigation provided significant information about antifungal activity and compounds isolated from two different strains of T. harzianum obtained from two different Himalayan locations.
authors.affiliation: a Division of Agricultural Chemicals, Indian Agricultural Research Institute , New Delhi 110 012 , India.
authors.forename: Vivek
authors.initials: V
authors.lastname: Ahluwalia
authors.forename: Jitendra
authors.initials: J
authors.lastname: Kumar
authors.forename: Virendra S
authors.initials: VS
authors.lastname: Rana
authors.forename: Om P
authors.initials: OP
authors.lastname: Sati
authors.forename: S
authors.initials: S
authors.lastname: Walia
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry*; D002845:Chromatography; D004875:Ergosterol / Q000302:isolation & purification; D015394:Molecular Structure; D019308:Palmitic Acid / Q000302:isolation & purification; D011728:Pyridones / Q000302:isolation & purification; D011809:Quinones / Q000302:isolation & purification; D064210:Secondary Metabolism; D013265:Stigmasterol / Q000302:isolation & purification; D014242:Trichoderma / Q000737:chemistry* / Q000145:classification
chemical_list: D000935:Antifungal Agents; D011728:Pyridones; D011809:Quinones; C576302:harzianopyridone; D019308:Palmitic Acid; D013265:Stigmasterol; D004875:Ergosterol
keywords: Trichoderma; antifungal activity; secondary metabolite