title: Effects of hormones on in vitro maturation of cattle oocytes.
abstract: The efficiency of cattle oocyte maturation in vitro was studied in protein-free MEM-α with hormones and in completely definite culture medium without hormones. Oocyte capacity to develop after fertilization to the morula/blastocyst and blastocyst stages served as a criterion of effective maturation. The increase in follicle-stimulating hormone concentration in the medium by one or two orders of magnitude in comparison with the "standard" level of 1 μg/ml deteriorated the development of embryos to the preimplantation stages. Serum gonadotropin from pregnant mares worked similarly as follicle-stimulating hormone. Oocytes that underwent maturation without hormones developed to the blastocyst stage, though the percentage of dividing embryos was significantly less and there was a trend to worse development of the embryos to the preimplantation stages.
authors.affiliation: All-Russian Research Institute of Physiology, Biochemistry, and Nutrition of Agricultural Animals, Russian Academy of Agricultural Sciences, Borovsk, Russia, sme.irina2011@yandex.ru.
authors.forename: I G
authors.initials: IG
authors.lastname: Smetanina
authors.forename: L V
authors.initials: LV
authors.lastname: Tatarinova
authors.forename: A S
authors.initials: AS
authors.lastname: Krivokharchenko
mesh_terms: D000818:Animals; D001755:Blastocyst; D002417:Cattle; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D066298:In Vitro Techniques; D009865:Oocytes / Q000166:cytology / Q000187:drug effects*
chemical_list: D005640:Follicle Stimulating Hormone