title: Novel in vitro inhibitory functions of potato tuber proteinaceous inhibitors.
abstract: Plant protease inhibitors are a structurally highly diverse and ubiquitous class of small proteins, which play various roles in plant development and defense against pests and pathogens. Particular isoforms inhibit in vitro proteases and other enzymes that are not their natural substrates, for example proteases that have roles in human diseases. Mature potato tubers are a rich source of several protease inhibitor families. Different cultivars have different inhibitor profiles. With the objective to explore the functional diversity of the natural diversity of potato protease inhibitors, we randomly selected and sequenced 9,600 cDNA clones originated from mature tubers of ten potato cultivars. Among these, 120 unique inhibitor cDNA clones were identified by homology searches. Eighty-eight inhibitors represented novel sequence variants of known plant protease inhibitor families. Most frequent were Kunitz-type inhibitors (KTI), potato protease inhibitors I and II (PIN), pectin methylesterase inhibitors, metallocarboxypeptidase inhibitors and defensins. Twenty-three inhibitors were functionally characterized after heterologous expression in the yeast Pichia pastoris. The purified recombinant proteins were tested for inhibitory activity on trypsin, eleven pharmacological relevant proteases and the non-proteolytic enzyme 5-lipoxygenase. Members of the KTI and PIN families inhibited pig pancreas elastase, β-Secretase, Cathepsin K, HIV-1 protease and potato 5-lipoxygenase. Our results demonstrate in vitro inhibitory diversity of small potato tuber proteins commonly known as protease inhibitors, which might have biotechnological or medical applications.
authors.affiliation: Department Plant Breeding and Genetics, Max-Planck Institute for Plant Breeding Research, Carl von LinnéWeg 10, 50829, Cologne, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Fischer
authors.forename: Markus
authors.initials: M
authors.lastname: Kuckenberg
authors.forename: Robin
authors.initials: R
authors.lastname: Kastilan
authors.forename: Jost
authors.initials: J
authors.lastname: Muth
authors.forename: Christiane
authors.initials: C
authors.lastname: Gebhardt
mesh_terms: D003001:Cloning, Molecular; D015723:Gene Library; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D020161:Physical Chromosome Mapping; D035281:Plant Tubers / Q000378:metabolism*; D011480:Protease Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification; D011198:Solanum tuberosum / Q000378:metabolism*
chemical_list: D016859:Lipoxygenase Inhibitors; D011480:Protease Inhibitors; D011993:Recombinant Fusion Proteins