title: Development and validation of a high-performance liquid chromatography-tandem mass spectrometric method for simultaneous determination of bupropion, quetiapine and escitalopram in human plasma.
abstract: In the present study, an effective high performance liquid chromatography-tandem mass spectrometric (HPLC/MS/MS) method was developed and validated to simultaneously determine bupropion (BUP), quetiapine (QUE) and escitalopram (ESC) in human plasma using carbidopa as the internal standard. Chromatographic separation was achieved on a Waters Sun Fire C18 column using reversed-phase chromatography. The MS/MS experiment was performed in positive ion multiple reaction monitoring mode to produce product ions of m/z 240.3 → 184.2 for BUP, 384.2 → 253.1 for QUE, 325.3 → 109.3 for ESC and 227.2 → 181.2 for the internal standard. The method showed good linearity (R(2)  ≥ 0.997), precision (relative standard deviation ≤7.5%), satisfactory intra- and interday accuracy (88.4-113.0%) and acceptable extraction recovery (87.2-115.0%), matrix effect (84.5.5-108.7%) and stability (92.3-103.5%). The method was successfully applied to determine the concentrations of BUP, QUE and ESC in human plasma samples.
authors.affiliation: Research Institute of Life Science and Department of Chemistry, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
authors.forename: Semin
authors.initials: S
authors.lastname: Park
authors.forename: Chul-Soo
authors.initials: CS
authors.lastname: Park
authors.forename: Sung Joong
authors.initials: SJ
authors.lastname: Lee
authors.forename: Boseok
authors.initials: B
authors.lastname: Cha
authors.forename: Young Ah
authors.initials: YA
authors.lastname: Cho
authors.forename: Yi
authors.initials: Y
authors.lastname: Song
authors.forename: Eun Ae
authors.initials: EA
authors.lastname: Yu
authors.forename: Gon-Sup
authors.initials: GS
authors.lastname: Kim
authors.forename: Jong Sung
authors.initials: JS
authors.lastname: Jin
authors.forename: A M
authors.initials: AM
authors.lastname: Abd El-Aty
authors.forename: H A
authors.initials: HA
authors.lastname: El-Banna
authors.forename: Ahmet
authors.initials: A
authors.lastname: Hacımüftüoğlu
authors.forename: Jae-Han
authors.initials: JH
authors.lastname: Shim
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Shin
mesh_terms: D000928:Antidepressive Agents / Q000097:blood*; D016642:Bupropion / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015283:Citalopram / Q000097:blood*; D006801:Humans; D000069348:Quetiapine Fumarate / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000928:Antidepressive Agents; D016642:Bupropion; D015283:Citalopram; D000069348:Quetiapine Fumarate
keywords: LC/MS/MS; bupropion; escitalopram; major depressive disorder; quetiapine