title: Addressable droplet microarrays for single cell protein analysis.
abstract: Addressable droplet microarrays are potentially attractive as a way to achieve miniaturised, reduced volume, high sensitivity analyses without the need to fabricate microfluidic devices or small volume chambers. We report a practical method for producing oil-encapsulated addressable droplet microarrays which can be used for such analyses. To demonstrate their utility, we undertake a series of single cell analyses, to determine the variation in copy number of p53 proteins in cells of a human cancer cell line.
authors.affiliation: Proxomics, Institute of Chemical Biology, Imperial College London, London, SW7 2AZ UK. ali.salehi-reyhani@imperial.ac.uk.
authors.forename: Ali
authors.initials: A
authors.lastname: Salehi-Reyhani
authors.forename: Edward
authors.initials: E
authors.lastname: Burgin
authors.forename: Oscar
authors.initials: O
authors.lastname: Ces
authors.forename: Keith R
authors.initials: KR
authors.lastname: Willison
authors.forename: David R
authors.initials: DR
authors.lastname: Klug
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D044085:Microfluidics; D040081:Protein Array Analysis; D059010:Single-Cell Analysis