title: Effect of chlorogenic acid (5-Caffeoylquinic Acid) isolated from Baccharis oxyodonta on the structure and pharmacological activities of secretory phospholipase A2 from Crotalus durissus terrificus.
abstract: The aim of this paper was to investigate the effect of chlorogenic acid (5-caffeoylquinic acid, 5CQA), isolated from Baccharis oxyodonta, on the structure and pharmacological effect of secretory phospholipase A2 (sPLA2) from Crotalus durissus terrificus. All in vitro and in vivo experiments were conducted using a purified sPLA2 compared under the same experimental conditions with sPLA2 : 5CQA. 5CQA induced several discrete modifications in the secondary structure and the hydrophobic characteristics of native sPLA2 that induced slight changes in the α-helical content, increase in the random coil structure, and decrease of fluorescence of native sPLA2. Moreover, 5CQA significantly decreased the enzymatic activity and the oedema and myonecrosis induced by native sPLA2. As the catalytic activity of sPLA2 plays an important role in several of its biological and pharmacological properties, antibacterial activity was used to confirm the decrease in its enzymatic activity by 5CQA, which induced massive bacterial cell destruction. We found that 5CQA specifically abolished the enzymatic activity of sPLA2 and induced discrete protein unfolding that mainly involved the pharmacological site of sPLA2. These results showed the potential application of 5CQA in the snake poisoning treatment and modulation of the pathological effect of inflammation induced by secretory PLA2.
authors.affiliation: UNESP, Campus Experimental do Litoral Paulista, PraÇa Infante Dom Henrique s/no, Bairro Parque Bitaru, 11330-900 São Vicente, SP, Brazil.
authors.forename: Daniela O
authors.initials: DO
authors.lastname: Toyama
authors.affiliation: Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, RuadoMatão 277, 05508-090 São Paulo, SP, Brazil.
authors.forename: Marcelo J P
authors.initials: MJ
authors.lastname: Ferreira
authors.affiliation: Escola de Engenharia, Curso de Química, Universidade Presbiteriana Mackenzie, Rua da Consolação 930, 01302-907 São Paulo, SP, Brazil.
authors.forename: Paulete
authors.initials: P
authors.lastname: Romoff
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Curso de Ciências Biológicas, Universidade Presbiteriana Mackenzie, Rua da Consolação 930, 01302-907 São Paulo, SP, Brazil.
authors.forename: Oriana A
authors.initials: OA
authors.lastname: Fávero
authors.affiliation: UNESP, Campus Experimental do Litoral Paulista, PraÇa Infante Dom Henrique s/no, Bairro Parque Bitaru, 11330-900 São Vicente, SP, Brazil.
authors.forename: Henrique H
authors.initials: HH
authors.lastname: Gaeta
authors.affiliation: UNESP, Campus Experimental do Litoral Paulista, PraÇa Infante Dom Henrique s/no, Bairro Parque Bitaru, 11330-900 São Vicente, SP, Brazil.
authors.forename: Marcos H
authors.initials: MH
authors.lastname: Toyama
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D031175:Baccharis / Q000737:chemistry*; D002726:Chlorogenic Acid / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000627:therapeutic use; D002942:Circular Dichroism; D017839:Crotalus / Q000378:metabolism*; D004487:Edema / Q000188:drug therapy / Q000473:pathology; D008297:Male; D051379:Mice; D008826:Microbial Sensitivity Tests; D054497:Phospholipases A2, Secretory / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000890:Anti-Infective Agents; D002726:Chlorogenic Acid; D054497:Phospholipases A2, Secretory