title: Synergistic effect of nanomaterials and BMP-2 signalling in inducing osteogenic differentiation of adipose tissue-derived mesenchymal stem cells.
abstract: The lack of complete understanding in the signalling pathways that control the osteogenic differentiation of mesenchymal stem cells hinders their clinical application in the reconstruction of large bone defects and non-union bone fractures. The aim of this study is to gain insight into the interactions of bone morphogenetic protein-2 (BMP-2) and bone biomimetic scaffolds in directing osteogenic differentiation of adipose tissue-derived mesenchymal stem cells (ASCs) and the underlying signalling pathways involved. We demonstrated that bioactive glass nanoparticles (nBG) incorporated polycaprolactone (PCL) coating on hydroxyapatite/β-tricalcium phosphate (HA/TCP) scaffold exerted a synergistic effect with 3days of BMP-2 treatment in promoting osteogenic gene expression levels (Runx-2, collagen I, osteopontin and bone sialoprotein) and alkaline phosphatase activity in ASCs. Furthermore, we revealed that the synergistic effect was mediated through a mechanism of activating β1-integrin and induction of Wnt-3a autocrine signalling pathways by nBG incorporated scaffold.
authors.affiliation: Biomaterials and Tissue Engineering Research Unit, School of AMME, the University of Sydney, Sydney, Australia. Electronic address: zufu.lu@sydney.edu.au.
authors.forename: ZuFu
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Biomaterials and Tissue Engineering Research Unit, School of AMME, the University of Sydney, Sydney, Australia.
authors.forename: Seyed-Iman
authors.initials: SI
authors.lastname: Roohani-Esfahani
authors.affiliation: Biomaterials and Tissue Engineering Research Unit, School of AMME, the University of Sydney, Sydney, Australia.
authors.forename: JiaoJiao
authors.initials: J
authors.lastname: Li
authors.affiliation: Biomaterials and Tissue Engineering Research Unit, School of AMME, the University of Sydney, Sydney, Australia. Electronic address: hala.zreiqat@sydney.edu.au.
authors.forename: Hala
authors.initials: H
authors.lastname: Zreiqat
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D032701:Biomimetics; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry; D002454:Cell Differentiation; D002470:Cell Survival; D024042:Collagen Type I / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D017886:Durapatite / Q000737:chemistry; D020869:Gene Expression Profiling; D005898:Glass / Q000737:chemistry; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism; D058574:Integrin-Binding Sialoprotein / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D050997:Nanomedicine / Q000379:methods; D049329:Nanostructures / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis; D053495:Osteopontin / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry; D015398:Signal Transduction; D060509:Wnt3A Protein / Q000378:metabolism
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D019012:Integrin beta1; D058574:Integrin-Binding Sialoprotein; D011091:Polyesters; C493709:RUNX2 protein, human; C555510:WNT3A protein, human; D060509:Wnt3A Protein; C485817:beta-tricalcium phosphate; D053495:Osteopontin; C016240:polycaprolactone; D017886:Durapatite
keywords: BMP-2; Bioglass; Osteogensis; Wnt; β1 integrin