title: A whole-cell bioreporter approach for the genotoxicity assessment of bioavailability of toxic compounds in contaminated soil in China.
abstract: A whole-cell bacterial bioreporter Acinetobacter baylyi strain ADP1_recA_lux that responds to genotoxins was employed to directly assess the adverse effects of the bioavailable fraction of mitomycin C (MMC), benzo[a]pyrene (BaP), chromium (VI) and lead (II) in amended soils and soil samples from two fragile areas in China without soil pre-treatment. The amended soils containing pollutants with the concentrations as low as 0.4 mg/kg MMC, 0.5 mg/kg BaP, 520 mg/kg Cr (VI) and 2072 mg/kg Pb (II) were found to be toxic. Soil particle-associated pollutants accounted for 86%, 100%, 29%, and 92% of the genotoxicity in the MMC, BaP, Cr (VI), and Pb (II) amended soil, respectively. The soils from contaminated sites were also valid to be genotoxic. The results suggest both free and soil particle-associated pollutants are bioavailable to soil organisms and a solid-phase contact bioreporter assay to soil contamination could provide a rapid screening tool for environmental risk assessment.
authors.affiliation: School of Environment, Tsinghua University, Beijing, 100084, PR China; State Key Joint Laboratory of Environment Simulation and Pollution Control, Beijing, 100084, PR China.
authors.forename: Yizhi
authors.initials: Y
authors.lastname: Song
authors.affiliation: School of Environment, Tsinghua University, Beijing, 100084, PR China; State Key Joint Laboratory of Environment Simulation and Pollution Control, Beijing, 100084, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.affiliation: School of Environment, Tsinghua University, Beijing, 100084, PR China.
authors.forename: Sicong
authors.initials: S
authors.lastname: Tian
authors.affiliation: School of Chemical and Environmental Engineering, China University of Mining & Technology, Beijing, 100083, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Tang
authors.affiliation: School of Environment, Tsinghua University, Beijing, 100084, PR China; State Key Joint Laboratory of Environment Simulation and Pollution Control, Beijing, 100084, PR China.
authors.forename: Zengjun
authors.initials: Z
authors.lastname: Liu
authors.affiliation: School of Environment, Tsinghua University, Beijing, 100084, PR China; State Key Joint Laboratory of Environment Simulation and Pollution Control, Beijing, 100084, PR China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Li
authors.affiliation: School of Chemical and Environmental Engineering, China University of Mining & Technology, Beijing, 100083, PR China.
authors.forename: Jianli
authors.initials: J
authors.lastname: Jia
authors.affiliation: Kroto Research Institute, University of Sheffield, Sheffield, S3 7HQ, UK.
authors.forename: Wei E
authors.initials: WE
authors.lastname: Huang
authors.affiliation: School of Environment, Tsinghua University, Beijing, 100084, PR China; State Key Joint Laboratory of Environment Simulation and Pollution Control, Beijing, 100084, PR China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Environment, Tsinghua University, Beijing, 100084, PR China; State Key Joint Laboratory of Environment Simulation and Pollution Control, Beijing, 100084, PR China. Electronic address: ligh@mail.tsinghua.edu.cn.
authors.forename: Guanghe
authors.initials: G
authors.lastname: Li
mesh_terms: D000150:Acinetobacter; D001564:Benzo(a)pyrene / Q000032:analysis / Q000633:toxicity; D001681:Biological Assay; D002681:China; D002857:Chromium / Q000032:analysis / Q000633:toxicity; D004249:DNA Damage; D004784:Environmental Monitoring / Q000379:methods*; D004787:Environmental Pollution / Q000706:statistics & numerical data; D015386:Hazardous Substances / Q000032:analysis / Q000633:toxicity; D009153:Mutagens / Q000032:analysis / Q000633:toxicity*; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000032:analysis / Q000633:toxicity*
chemical_list: D015386:Hazardous Substances; D009153:Mutagens; D012987:Soil; D012989:Soil Pollutants; D002857:Chromium; D001564:Benzo(a)pyrene
keywords: Bioavailability; Genotoxicity; PAHs; Soil; Whole-cell bioreporter