title: The redundant aminotransferases in lysine and arginine synthesis and the extent of aminotransferase redundancy in Escherichia coli.
abstract: Aminotransferases can be redundant or promiscuous, but the extent and significance of these properties is not known in any organism, even in Escherichia coli. To determine the extent of redundancy, it was first necessary to identify the redundant aminotransferases in arginine and lysine synthesis, and then complement all aminotransferase-deficient mutants with genes for all aminotransferases. The enzymes with N-acetylornithine aminotransferase (ACOAT) activity in arginine synthesis were ArgD, AstC, GabT and PuuE; the major anaerobic ACOAT was ArgD. The major enzymes with N-succinyl-l,l-diaminopimelate aminotransferase (SDAP-AT) activity in lysine synthesis were ArgD, AstC, and SerC. Seven other aminotransferases, when overproduced, complemented the defect in a triple mutant. Lysine availability did not regulate synthesis of the major SDAP-ATs. Complementation analysis of mutants lacking aminotransferases showed that the SDAP-ATs and alanine aminotransferases were exceptionally redundant, and it is proposed that this redundancy may ensure peptidoglycan synthesis. An overview of all aminotransferase reactions indicates that redundancy and broad specificity are common properties of aminotransferases.
authors.affiliation: Department of Molecular and Cell Biology, The University of Texas at Dallas, Richardson, TX, 75080, USA.
authors.forename: Piyush Behari
authors.initials: PB
authors.lastname: Lal
authors.forename: Barbara L
authors.initials: BL
authors.lastname: Schneider
authors.forename: Khoan
authors.initials: K
authors.lastname: Vu
authors.forename: Larry
authors.initials: L
authors.lastname: Reitzer
mesh_terms: D001120:Arginine / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D005816:Genetic Complementation Test; D008239:Lysine / Q000096:biosynthesis*; D010457:Peptidoglycan / Q000096:biosynthesis; D000637:Transaminases / Q000235:genetics / Q000378:metabolism*
chemical_list: D010457:Peptidoglycan; D001120:Arginine; D000637:Transaminases; D008239:Lysine