title: The mitochondrial protein import component, TRANSLOCASE OF THE INNER MEMBRANE17-1, plays a role in defining the timing of germination in Arabidopsis.
abstract: In Arabidopsis (Arabidopsis thaliana), small gene families encode multiple isoforms for many of the components of the mitochondrial protein import apparatus. There are three isoforms of the TRANSLOCASE OF THE INNER MEMBRANE17 (Tim17). Transcriptome analysis indicates that AtTim17-1 is only detectable in dry seed. In this study, two independent transfer DNA insertional mutant lines of tim17-1 exhibited a germination-specific phenotype, showing a significant increase in the rate of germination. Microarray analyses revealed that Attim17-1 displayed alterations in the temporal sequence of transcriptomic events during germination, peaking earlier compared with the wild type. Promoter analysis of AtTim17-1 further identified an abscisic acid (ABA)-responsive element, which binds ABA-responsive transcription factors, acting to repress the expression of AtTim17-1. Attim17-1 dry seeds contained significantly increased levels of ABA and gibberellin, 2- and 5-fold, respectively. These results support the model that mitochondrial biogenesis is regulated in a tight temporal sequence of events during germination and that altering mitochondrial biogenesis feeds back to alter the germination rate, as evidenced by the altered levels of the master regulatory hormones that define germination.
authors.affiliation: Department of Botany, School of Life Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia (Y.W., S.R.L., A.I., R.N., J.W.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (O.v.A, S.K.-J., V.U., M.v.d.M., O.D., M.W.M.).
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Botany, School of Life Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia (Y.W., S.R.L., A.I., R.N., J.W.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (O.v.A, S.K.-J., V.U., M.v.d.M., O.D., M.W.M.).
authors.forename: Simon R
authors.initials: SR
authors.lastname: Law
authors.affiliation: Department of Botany, School of Life Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia (Y.W., S.R.L., A.I., R.N., J.W.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (O.v.A, S.K.-J., V.U., M.v.d.M., O.D., M.W.M.).
authors.forename: Aneta
authors.initials: A
authors.lastname: Ivanova
authors.affiliation: Department of Botany, School of Life Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia (Y.W., S.R.L., A.I., R.N., J.W.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (O.v.A, S.K.-J., V.U., M.v.d.M., O.D., M.W.M.).
authors.forename: Olivier
authors.initials: O
authors.lastname: van Aken
authors.affiliation: Department of Botany, School of Life Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia (Y.W., S.R.L., A.I., R.N., J.W.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (O.v.A, S.K.-J., V.U., M.v.d.M., O.D., M.W.M.).
authors.forename: Szymon
authors.initials: S
authors.lastname: Kubiszewski-Jakubiak
authors.affiliation: Department of Botany, School of Life Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia (Y.W., S.R.L., A.I., R.N., J.W.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (O.v.A, S.K.-J., V.U., M.v.d.M., O.D., M.W.M.).
authors.forename: Vindya
authors.initials: V
authors.lastname: Uggalla
authors.affiliation: Department of Botany, School of Life Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia (Y.W., S.R.L., A.I., R.N., J.W.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (O.v.A, S.K.-J., V.U., M.v.d.M., O.D., M.W.M.).
authors.forename: Margaretha
authors.initials: M
authors.lastname: van der Merwe
authors.affiliation: Department of Botany, School of Life Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia (Y.W., S.R.L., A.I., R.N., J.W.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (O.v.A, S.K.-J., V.U., M.v.d.M., O.D., M.W.M.).
authors.forename: Owen
authors.initials: O
authors.lastname: Duncan
authors.affiliation: Department of Botany, School of Life Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia (Y.W., S.R.L., A.I., R.N., J.W.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (O.v.A, S.K.-J., V.U., M.v.d.M., O.D., M.W.M.).
authors.forename: Reena
authors.initials: R
authors.lastname: Narsai
authors.affiliation: Department of Botany, School of Life Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia (Y.W., S.R.L., A.I., R.N., J.W.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (O.v.A, S.K.-J., V.U., M.v.d.M., O.D., M.W.M.).
authors.forename: James
authors.initials: J
authors.lastname: Whelan
authors.affiliation: Department of Botany, School of Life Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia (Y.W., S.R.L., A.I., R.N., J.W.); andAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (O.v.A, S.K.-J., V.U., M.v.d.M., O.D., M.W.M.) monika.murcha@uwa.edu.au.
authors.forename: Monika W
authors.identifier: http://orcid.org/0000-0002-3689-6158
authors.initials: MW
authors.lastname: Murcha
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D018525:Germination / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D005875:Gibberellins / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D009154:Mutation; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms; D012639:Seeds / Q000187:drug effects / Q000502:physiology; D013997:Time Factors
chemical_list: D029681:Arabidopsis Proteins; D005875:Gibberellins; D026901:Membrane Transport Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D020033:Protein Isoforms; C501430:TIM17 protein, Arabidopsis; D000040:Abscisic Acid