title: Attachment of Escherichia coli to Listeria monocytogenes for pediocin-mediated killing.
abstract: Listeria phage endolysin cell wall-binding domain (CBD) from the Listeria phage A500 was fused with flagellar subunit FliC in Escherichia coli, aiming at binding of E. coli cells to Listeria cells, followed by enhanced killing of Listeria by pediocin production. FliC::CBD chimeric flagella were expressed and detected by Western blot. However, only few chimeric flagella could be isolated from the recombinant cells compared with sufficient amount of wild-type flagella obtained from the host cells. Interestingly, wild-type flagella extract showed capacity of binding Listeria cells. Pediocin-secreting E. coli cells with Listeria-binding flagella killed approximately 40 % of the Listeria cells, whereas cell-free spent growth medium with the same pediocin concentration only inhibited Listeria growth. These results suggested that binding the Listeria to bacteriocin-secreting cells improves killing.
authors.affiliation: College of Food Science and Bioengineering, Tianjin Agricultural University, 22 Jinjing Road, 300384, Tianjin, People's Republic of China.
authors.forename: Shanna
authors.initials: S
authors.lastname: Liu
authors.forename: Timo M
authors.initials: TM
authors.lastname: Takala
authors.forename: Justus
authors.initials: J
authors.lastname: Reunanen
authors.forename: Ossian
authors.initials: O
authors.lastname: Saris
authors.forename: Per E J
authors.initials: PE
authors.lastname: Saris
mesh_terms: D000898:Antibiosis; D001422:Bacterial Adhesion; D001430:Bacteriocins / Q000096:biosynthesis / Q000378:metabolism*; D004926:Escherichia coli / Q000502:physiology*; D005407:Flagella / Q000235:genetics / Q000378:metabolism; D008089:Listeria monocytogenes / Q000502:physiology*
chemical_list: D001430:Bacteriocins