title: Structural features and complement fixing activity of polysaccharides from Codonopsis pilosula Nannf. var. modesta L.T.Shen roots.
abstract: Two pectic polysaccharides, 50 WCP-II-I and 100 WCP-II-I, were obtained from 50 and 100 °C water extracts of Codonopsis pilosula roots by ion exchange chromatography and gel filtration. The study of the sub-fractions obtained after pectinase degradation showed that the complement fixation activities of these pectins are expressed mainly by their ramified regions. The structure studies of native and sub-fractions showed the 50 WCP-II-I is a pectic polysaccharide, with long homogalacturonan regions (some of the galacturonic acid units were methyl esterified), interrupted by one short rhamnogalacturonan I (RG-I) region. The side chains of the RG-I region are arabinogalactan type I (AG-I) and type II (AG-II) attached on position 4 of rhamnose. The 100 WCP-II-I has two main ramified regions, one is galacturonan region with AG-I side chain on position 2 of GalA, and the other one is RG-I region with AG-II side chain on position 4 of Rha.
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway. Electronic address: yuanfeng.zou@farmasi.uio.no.
authors.forename: Yuan-Feng
authors.initials: YF
authors.lastname: Zou
authors.affiliation: Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, College of Agronomy, Sichuan Agricultural University, Wenjiang, 611130, PR China.
authors.forename: Xing-Fu
authors.initials: XF
authors.lastname: Chen
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway.
authors.forename: Karl Egil
authors.initials: KE
authors.lastname: Malterud
authors.affiliation: Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, 0315 Oslo, Norway.
authors.forename: Frode
authors.initials: F
authors.lastname: Rise
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway.
authors.forename: Hilde
authors.initials: H
authors.lastname: Barsett
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway.
authors.forename: Kari Tvete
authors.initials: KT
authors.lastname: Inngjerdingen
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway.
authors.forename: Terje Einar
authors.initials: TE
authors.lastname: Michaelsen
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway.
authors.forename: Berit Smestad
authors.initials: BS
authors.lastname: Paulsen
mesh_terms: D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D029745:Codonopsis / Q000737:chemistry*; D005685:Galactans / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D010368:Pectins; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure; D014867:Water / Q000737:chemistry
chemical_list: D005685:Galactans; D006603:Hexuronic Acids; D011134:Polysaccharides; D014867:Water; C007819:galacturonic acid; D010368:Pectins; C005653:arabinogalactan
keywords: Codonopsis pilosula; Complement fixation activity; Polysaccharides; Rhamnogalacturonan I