title: Evaluation of the application of enemas containing sucralfate in tissue content of neutral and acid mucins in experimental model of diversion colitis.
abstract: OBJECTIVE
  To evaluate the effects of sucralfate on tissue content of neutral and acids mucins in rats with diversion colitis.
  
  
  METHODS
  Thirty-six rats were submitted to a proximal right colostomy and a distal mucous fistula. They were divided into two groups according to sacrifice to be performed two or four weeks after intervention. Each group was divided into three subgroups according daily application of enemas containing saline, sucralfate at 1.0 g/kg/day or 2.0 g/kg/day. Colitis was diagnosed by histological analysis and neutral and acid mucins by Periodic Acid Schiff and Alcian Blue techniques, respectively. The contents of mucins were quantified by computer-assisted image analysis. Student's t paired and ANOVA test were used to compare the contents of both types of mucins among groups, and to verify the variance with time, establishing level of signification of 5% for both (p<0.05).
  
  
  RESULTS
  Enemas containing sucralfate improves the inflammation and increases the tissue contents of neutral and acid mucins. The content of neutral mucins does not change with the time or concentration of sucralfate used, while acid mucins increases with concentration and time of intervention.
  
  
  CONCLUSIONS
  Sucralfate enemas improve the inflammatory process and increase the tissue content of neutral and acid mucins in colon without fecal stream.
authors.affiliation: Sao Francisco University, Bragança Paulista, SP, Brazil.
authors.forename: Felipe Mendonça
authors.initials: FM
authors.lastname: Chaim
authors.affiliation: School of Medicine, USF, Bragança Paulista, SP, Brazil.
authors.forename: Daniela Tiemi
authors.initials: DT
authors.lastname: Sato
authors.affiliation: School of Medicine, USF, Bragança Paulista, SP, Brazil.
authors.forename: Murilo Rocha
authors.initials: MR
authors.lastname: Rodrigues
authors.affiliation: School of Medicine, USF, Bragança Paulista, SP, Brazil.
authors.forename: Alice Moreira
authors.initials: AM
authors.lastname: Dias
authors.affiliation: School of Medicine, USF, Bragança Paulista, SP, Brazil.
authors.forename: Paulo Pedroso
authors.initials: PP
authors.lastname: Silveira Júnior
authors.affiliation: USF, Bragança Paulista, SP, Brazil.
authors.forename: José Aires
authors.initials: JA
authors.lastname: Pereira
authors.affiliation: USF, Bragança Paulista, SP, Brazil.
authors.forename: Carlos Augusto Real
authors.initials: CA
authors.lastname: Martinez
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology / Q000627:therapeutic use*; D003092:Colitis / Q000188:drug therapy* / Q000473:pathology; D003106:Colon / Q000187:drug effects / Q000473:pathology; D004195:Disease Models, Animal; D004733:Enema / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D009077:Mucins / Q000032:analysis* / Q000187:drug effects; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013392:Sucralfate / Q000494:pharmacology / Q000627:therapeutic use*; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D000897:Anti-Ulcer Agents; D008562:Membrane Glycoproteins; D009077:Mucins; D013392:Sucralfate