title: Performance of the SD Bioline TB Ag MPT64 Rapid test for quick confirmation of Mycobacterium bovis isolates from animals.
abstract: Mycobacterium (M.) bovis, a bacterium in the M. tuberculosis complex, is a causative agent of bovine tuberculosis, a contagious disease of animals. Mycobacterial culture is the gold standard for diagnosing bovine tuberculosis, but this technique is laborious and time-consuming. In the present study, performance of the SD Bioline TB Ag MPT4 Rapid test, an immunochromatographic assay, was evaluated using reference bacterial strains and M. bovis field isolates collected from animals. The SD MPT64 Rapid test produced positive results for 95.5% (63/66) of the M. bovis isolates from cattle and 97.9% (46/47) of the isolates from deer. Additionally, the test had a sensitivity of 96.5% (95% CI, 91.2-99.0), specificity of 100% (95% CI, 96.7-100.0), positive predictive value of 100% (95% CI, 96.7-100.0), and negative predictive value of 92.9% (95% CI, 82.7-98.0) for M. bovis isolates. In conclusion, the SD MPT64 Rapid test is simple to use and may be useful for quickly confirming the presence of M. bovis in animals.
authors.affiliation: Chungbuk Veterinary Service Laboratory, Chungju 380-230, Korea.
authors.forename: Hyeon-Seop
authors.initials: HS
authors.lastname: Byeon
authors.forename: Mi Jung
authors.initials: MJ
authors.lastname: Ji
authors.forename: Shin-Seok
authors.initials: SS
authors.lastname: Kang
authors.forename: Sang Woo
authors.initials: SW
authors.lastname: Kim
authors.forename: Seung-Cheol
authors.initials: SC
authors.lastname: Kim
authors.forename: Song-Yong
authors.initials: SY
authors.lastname: Park
authors.forename: Geehyuk
authors.initials: G
authors.lastname: Kim
authors.forename: Jiro
authors.initials: J
authors.lastname: Kim
authors.forename: Jang-Eun
authors.initials: JE
authors.lastname: Cho
authors.forename: Bok Kyung
authors.initials: BK
authors.lastname: Ku
authors.forename: Jae-Myung
authors.initials: JM
authors.lastname: Kim
authors.forename: Bo-Young
authors.initials: BY
authors.lastname: Jeon
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000382:microbiology; D002846:Chromatography, Affinity / Q000379:methods / Q000662:veterinary*; D003670:Deer; D009163:Mycobacterium bovis / Q000145:classification / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*
keywords: Mycobacterium bovis; SD MPT64 Rapid test; animals