title: Synthesis of thiolated glycosaminoglycans and grafting to solid surfaces.
abstract: Glycosaminoglycans (GAGs) with varying degree of sulfation were chemically modified to obtain thiolated analogues (tGAGs) for subsequent surface grafting on vinyl-terminated self-assembled monolayers. Thiolation was achieved by the use of the disulfide containing crosslinker 3,3'-dithiobis(propanoic hydrazide) and subsequent reduction of the disulfide with dithiothreitol. Two different molar ratios of the crosslinker were used for conjugation. The tGAGs were characterized by (1)H-NMR, Raman and flow-field-flow-fractionation (A4F) to determine the chemical composition, structure and molecular weight of the products. Ellman's reagent was used to quantify the thiol concentration of tGAGs. The tGAGs were immobilized onto vinyl-terminated glass and silicon via thiol-ene reaction. This was achieved by homogeneous immobilization from solution as well as with microcontact printing and exposure to UV light. The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
authors.affiliation: Biomedical Materials Group, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, D-06099 Halle Saale, Germany. Electronic address: Alexander.Koewitsch@pharmazie.uni-halle.de.
authors.forename: Alexander
authors.initials: A
authors.lastname: Köwitsch
authors.affiliation: Biomedical Materials Group, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, D-06099 Halle Saale, Germany. Electronic address: Jurado.Abreu@hotmail.com.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Jurado Abreu
authors.affiliation: Biomedical Materials Group, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, D-06099 Halle Saale, Germany. Electronic address: Ankurchhalotre@gmail.com.
authors.forename: Ankur
authors.initials: A
authors.lastname: Chhalotre
authors.affiliation: Institute of Plant and Wood Chemistry, Dresden University of Technology, Pienner Strasse 19, D-01737 Tharandt, Germany. Electronic address: Martin.hielscher@forst.tu-dresden.de.
authors.forename: Martin
authors.initials: M
authors.lastname: Hielscher
authors.affiliation: Institute of Plant and Wood Chemistry, Dresden University of Technology, Pienner Strasse 19, D-01737 Tharandt, Germany. Electronic address: Sfischer@forst.tu-dresden.de.
authors.forename: Steffen
authors.initials: S
authors.lastname: Fischer
authors.affiliation: Pharmaceutical Technology Group, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Wolfgang-Langenbeck-Strasse 4, D-06099 Halle Saale, Germany. Electronic address: Karsten.maeder@pharmazie.uni-halle.de.
authors.forename: Karsten
authors.initials: K
authors.lastname: Mäder
authors.affiliation: Biomedical Materials Group, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, D-06099 Halle Saale, Germany. Electronic address: Thomas.groth@pharmazie.uni-halle.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Groth
mesh_terms: D006025:Glycosaminoglycans / Q000138:chemical synthesis* / Q000737:chemistry*; D018613:Microscopy, Confocal; D013499:Surface Properties
chemical_list: D006025:Glycosaminoglycans
keywords: Ellipsometry; Glycosaminoglycan; Microcontact printing (μcp); Surface grafting; Thiol-ene chemistry; Water contact angle measurements (WCA)