title: [Determination of polysaccharides content of Gentiana farreri from different producing areas based on anthrone-sulfuric acid method].
abstract: Study a method for the detemination of the content of polysaccharides in Gentiana farreri, and analysis of the content of polysaccharides from different producing areas. The results showed that using the anthrone-sulfuric acid method, simple operation, accurate result. Sample was measured at 620 nm absorbance after anthrone-sulfuric acid color, at this wavelength, solution absorption and glucose showed a good linear relationship; The linearity was in the range of 0.01-0.07 g x L(-1) (r = 0.996 7). The recovery rate was 99.41%, with RSD of 2.0%. Considering the experimental conditions, to determine the solid-liquid ratio 1:60, extracting time 50 min, concentration of ethanol 80%. The mass fraction of polysaccharides was the highest to reached 0.743% in G. farreri from Gansu Xiahe. This experiment has laid a good foundation for further study on G. farreri.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Fu-Xing
authors.initials: FX
authors.lastname: Wang
authors.forename: Jia-Yan
authors.initials: JY
authors.lastname: He
authors.forename: Hang-Lin
authors.initials: HL
authors.lastname: Zhang
mesh_terms: D000873:Anthracenes / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D030012:Gentiana / Q000737:chemistry* / Q000254:growth & development; D005843:Geography; D016014:Linear Models; D011134:Polysaccharides / Q000032:analysis*; D015203:Reproducibility of Results; D013464:Sulfuric Acids / Q000737:chemistry*; D013997:Time Factors; D000095322:Anthrones
chemical_list: D000873:Anthracenes; D011134:Polysaccharides; D013464:Sulfuric Acids; C004522:anthrone; D000095322:Anthrones; C033158:sulfuric acid