title: Tegillarca granosa extract Haishengsu induces apoptosis in human hepatocellular carcinoma cell line BEL-7402 via Fas-signaling pathways.
abstract: This study was designed to investigate the apoptosis-inducing properties of Tegillarca granosa extract Haishengsu (HSS) in human hepatocellular carcinoma cell line BEL-7402. Proliferation inhibition of the human hepatocellular carcinoma BEL-7402 cells was determined by the MTT assay, and the cell viability was determined by trypan blue dye exclusion assay. Apoptosis of BEL-7402 cells was demonstrated by fluorescence microscope with flow cytometry with Annexin V-FITC/PI double staining and Hoechst 33258 staining. Western blot analysis and RT-PCR were used to determine the expression levels of Fas. Expressions of caspase-8 and caspase-3 were examined by caspase activity assay and western blot analysis. HSS inhibited the proliferation of human hepatocellular carcinoma BEL-7402 cells in a dose- and time-dependent manner. Our results showed HSS had positive effect on apoptosis through flow cytometry assay and fluorescence microscope. The expressions of Fas protein and mRNA were up-regulated following the treatment. Caspase-8 and caspase-3 were activated in the cells cultured with HSS. In conclusion, HSS induced apoptosis of human hepatocellular carcinoma BEL-7402 cells. The apoptosis was associated with the up-regulation of Fas and the activations of caspase-8 and caspase-3.
authors.affiliation: Medical College of Qingdao University, Qingdao, 266071, China, chen-xuehong@163.com.
authors.forename: Xuehong
authors.initials: X
authors.lastname: Chen
authors.forename: Yantao
authors.initials: Y
authors.lastname: Han
authors.forename: Songmei
authors.initials: S
authors.lastname: Zhan
authors.forename: Chunbo
authors.initials: C
authors.lastname: Wang
authors.forename: Shouguo
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis; D049893:Arcidae / Q000737:chemistry*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D045744:Cell Line, Tumor; D045424:Complex Mixtures / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D008516:Medicine, Chinese Traditional; D015398:Signal Transduction; D019014:fas Receptor / Q000378:metabolism*
chemical_list: D045424:Complex Mixtures; D019014:fas Receptor