title: Pyrosequencing assessment of rhizosphere fungal communities from a soybean field.
abstract: Soil fungal communities play essential roles in soil ecosystems, affecting plant growth and health. Rhizosphere bacterial communities have been shown to undergo dynamic changes during plant growth. This study utilized 454 pyrosequencing to analyze rhizosphere fungal communities during soybean growth. Members of the Ascomycota and Basiodiomycota dominated in all soils. There were no statistically significant changes at the phylum level among growth stages or between bulk and rhizosphere soils. In contrast, the relative abundance of small numbers of operational taxonomic units, 4 during growth and 28 between bulk and rhizosphere soils, differed significantly. Clustering analysis revealed that rhizosphere fungal communities were different from bulk fungal communities during growth stages of soybeans. Taken together, these results suggest that in contrast to rhizosphere bacterial communities, most constituents of rhizosphere fungal communities remained stable during soybean growth.
authors.affiliation: a Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Uji 611-0011, Japan.
authors.forename: Akifumi
authors.initials: A
authors.lastname: Sugiyama
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Ueda
authors.forename: Hisabumi
authors.initials: H
authors.lastname: Takase
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Yazaki
mesh_terms: D044822:Biodiversity; D016000:Cluster Analysis; D005658:Fungi / Q000145:classification* / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D058441:Rhizosphere; D012988:Soil Microbiology; D013025:Glycine max / Q000254:growth & development / Q000382:microbiology*
chemical_list: D012337:RNA, Ribosomal, 18S
keywords: communauté fongique; fungal community; pyrosequencing; pyroséquençage; rhizosphere; rhizosphère; soja; soybean