title: Archaeosomes can efficiently deliver different types of cargo into epithelial cells grown in vitro.
abstract: Archaeosomes are a type of liposomes prepared from the polar lipids of various Archaeobacteria. These have unique structural features that increase the lipid bilayer's stability even under high temperatures, low or high pH, presence of phospholipases and bile salts. This makes them ideal as basis for the development of new drug, gene and vaccine delivery systems. In this study we prepared large unilamellar archaeosomes (400nm size) from Aeropyrum pernix K1 and demonstrated their potential as base for the development of an efficient and universal system for drug or therapy delivery to epithelial cells. Our archaeosomes may be used to deliver small fluorescent molecules (calcein), smaller proteins (60kDa listeriolysin), large protein aggregates (e.g. keratin 14) and plasmid DNA, into epithelial cells grown in culture. The delivery efficiency for small molecules is already quite high at this initial stage of development, around 40%. Our unilamellar archaeosomes are also not toxic to keratinocytes even at high doses (500μg/ml).
authors.affiliation: National Institute of Chemistry, Hajdrihova 19, Ljubljana, Slovenia Medical Center for Molecular Biology, Faculty of Medicine, University of Ljubljana, Slovenia.
authors.forename: Apolonija Bedina
authors.initials: AB
authors.lastname: Zavec
authors.affiliation: Biotechnical Faculty, University of Ljubljana, Slovenia.
authors.forename: Ajda
authors.initials: A
authors.lastname: Ota
authors.affiliation: National Institute of Chemistry, Hajdrihova 19, Ljubljana, Slovenia Medical Center for Molecular Biology, Faculty of Medicine, University of Ljubljana, Slovenia.
authors.forename: Tina
authors.initials: T
authors.lastname: Zupancic
authors.affiliation: Medical Centre for Molecular Biology, Faculty of Medicine, University of Ljubljana, Slovenia.
authors.forename: Radovan
authors.initials: R
authors.lastname: Komel
authors.affiliation: Biotechnical Faculty, University of Ljubljana, Slovenia.
authors.forename: Nataša Poklar
authors.initials: NP
authors.lastname: Ulrih
authors.affiliation: Medical Centre for Molecular Biology, Faculty of Medicine, University of Ljubljana, Slovenia. Electronic address: mirjana.liovic@mf.uni-lj.si.
authors.forename: Mirjana
authors.initials: M
authors.lastname: Liovic
mesh_terms: D041522:Aeropyrum; D001427:Bacterial Toxins / Q000008:administration & dosage; D002460:Cell Line; D045744:Cell Line, Tumor; D004247:DNA / Q000008:administration & dosage; D016503:Drug Delivery Systems; D004847:Epithelial Cells / Q000378:metabolism; D005452:Fluoresceins / Q000008:administration & dosage; D006360:Heat-Shock Proteins / Q000008:administration & dosage; D006460:Hemolysin Proteins / Q000008:administration & dosage; D006801:Humans; D053547:Keratin-14 / Q000235:genetics; D015603:Keratinocytes / Q000378:metabolism; D008055:Lipids / Q000737:chemistry; D008081:Liposomes; D010957:Plasmids; D011994:Recombinant Proteins / Q000008:administration & dosage
chemical_list: D001427:Bacterial Toxins; D005452:Fluoresceins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D053547:Keratin-14; D008055:Lipids; D008081:Liposomes; D011994:Recombinant Proteins; D004247:DNA; C038705:hlyA protein, Listeria monocytogenes; C007740:fluorexon
keywords: Archaeosomes; Delivery; Epithelial cells; Liposomes; Protein