title: Solid state fermentation and production of rifamycin SV using Amycolatopsis mediterranei.
abstract: Production of Rifamycin SV from cheaper agro-industrial by-products using mutant strain of Amycolatopsis mediterranei OVA5-E7 in solid state fermentation (SSF) was optimized. Among the agro-based substrates used, ragi bran was found suitable for maximizing the yield of Rifamycin SV (1310 mg 100 g(-1) ds). The yield can be further enhanced to 19·7 g Kg(-1) of dry substrate by supplementing the substrate with deoiled cotton cake (10% w/w) using optimized fermentation parameters such as maintaining 80% moisture, pH 7·0, 30°C incubation temperature, inoculum 25% v/w and carrying the solid state fermenting for 9 days. Manipulating these seven specifications, the end product yield achieved in our experimentation was 20 g of Rifamycin SV Kg(-1) ds. Eventually, an overall 5-fold improvement in Rifamycin SV production was achieved.
  
  
  CONCLUSIONS
  Antibiotics such as rifamycin are broad-spectrum antimicrobial drugs used in large-scale worldwide as human medicine towards controlling diseases. Amycolatopsis mediterranei strain which produces this antibiotic was earlier used in submerged fermentation yielded lower amounts of rifamycin. By employing cheaper agro-industrial by-products, we produced upto 20 g rifamycin SV per Kg dry substrate used under optimized solid state fermentation conditions. Keeping in view, the role of rifamycin in meeting the medical demands of world's increasing population; we successfully used an improved strain on cheaper substrates with optimized fermentation parameters and achieved a 5-fold improvement in rifamycin SV production.
authors.affiliation: Department of Microbiology, Osmania University, Hyderabad, India.
authors.forename: M
authors.initials: M
authors.lastname: Nagavalli
authors.forename: S P D
authors.initials: SP
authors.lastname: Ponamgi
authors.forename: V
authors.initials: V
authors.lastname: Girijashankar
authors.forename: L
authors.initials: L
authors.lastname: Venkateswar Rao
mesh_terms: D000192:Actinomycetales / Q000378:metabolism*; D001420:Bacteria, Aerobic; D019149:Bioreactors; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D012294:Rifamycins / Q000096:biosynthesis*; D013696:Temperature
chemical_list: D003470:Culture Media; D012294:Rifamycins; C023808:rifamycin SV
keywords: Amycolatopsis mediterranei; Rifamycin; agro-industrial by-products; fermentation optimization; ragi bran; solid state fermentation; strain improvement