title: Synthesis of [13C4]-labeled ∆9-tetrahydrocannabinol and 11-nor-9-carboxy-∆9-tetrahydrocannabinol as internal standards for reducing ion suppressing/alteration effects in LC/MS-MS quantification.
abstract: (-)-∆9-Tetrahydrocannabinol is the principal psychoactive component of the cannabis plant and also the active ingredient in some prescribed drugs. To detect and control misuse and monitor administration in clinical settings, reference samples of the native drugs and their metabolites are needed. The accuracy of liquid chromatography/mass spectrometric quantification of drugs in biological samples depends among others on ion suppressing/alteration effects. Especially, 13C-labeled drug analogues are useful for minimzing such interferences. Thus, to provide internal standards for more accurate quantification and for identification purpose, synthesis of [13C4]-∆9-tetrahydro-cannabinol and [13C4]-11-nor-9-carboxy-∆9-tetrahydrocannabinol was developed via [13C4]-olivetol. Starting from [13C4]-olivetol the synthesis of [13C4]-11-nor-9-carboxy-∆9-tetrahydrocannabinol was shortened from three to two steps by employing nitromethane as a co-solvent in condensation with (+)-apoverbenone.
authors.affiliation: Department of Chemistry, Norwegian University of Science and Technology, Høgskoleringen 5, 7491 Trondheim, Norway. Morten.Karlsen@chiron.no.
authors.forename: Morten
authors.initials: M
authors.lastname: Karlsen
authors.affiliation: Chiron AS, Stiklestadveien 1, 7041 Trondheim 7041, Norway. Huiling.Liu@chiron.no.
authors.forename: Huiling
authors.initials: H
authors.lastname: Liu
authors.affiliation: Chiron AS, Stiklestadveien 1, 7041 Trondheim 7041, Norway. Jon.Johansen@chiron.no.
authors.forename: Jon Eigill
authors.initials: JE
authors.lastname: Johansen
authors.affiliation: Department of Chemistry, Norwegian University of Science and Technology, Høgskoleringen 5, 7491 Trondheim, Norway. bard.helge.hoff@chem.ntnu.no.
authors.forename: Bård Helge
authors.initials: BH
authors.lastname: Hoff
mesh_terms: D002247:Carbon Isotopes; D002853:Chromatography, Liquid / Q000379:methods*; D013759:Dronabinol / Q000031:analogs & derivatives / Q000138:chemical synthesis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002247:Carbon Isotopes; D013759:Dronabinol