title: Tailoring of the dopamine coated surface with VEGF loaded heparin/poly-L-lysine particles for anticoagulation and accelerate in situ endothelialization.
abstract: Coronary artery disease is a great threat to human health and is the leading killer worldwide. Percutaneous coronary intervention is the most effective therapy; however, thrombus, and restenosis caused by endothelium injury continue to be problematic after treatment. It is widely accepted that surface biofunctional modification to improve blood compatibility and accelerate endothelialization may be an effective approach to prevent the occurrence of adverse cardiac events. In this study, novel VEGF-loaded heparin/poly-L-lysine (Hep/PLL) particles were developed and immobilized on a dopamine coated titanium surface. The size, distribution, zeta potential, and morphology of the prepared particles were subsequently characterized. The influence of changes in the surface physicochemical properties after particle immobilization was assessed for blood compatibility and cytocompatibility. Surface-modified VEGF-loaded particles significantly inhibited platelet adhesion and activation and were effective in promoting the proliferation and survival of endothelial progenitor cells and endothelial cells. Moreover, Hep/PLL particles were also beneficial for controlling the long-term release of VEGF, which may facilitate endothelium regeneration. In conclusion, VEGF-loaded Hep/PLL particles were successfully immobilized on the Ti surface, and the biocompatibility was significantly improved. This study demonstrates a potential application for the multifunctional modification of stent surfaces for clinical use.
authors.affiliation: Key Laboratory of Advanced Technology of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu, 610031, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zeng
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Junying
authors.initials: J
authors.lastname: Chen
authors.forename: Nan
authors.initials: N
authors.lastname: Huang
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D004298:Dopamine / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010314:Partial Thromboplastin Time; D010316:Particle Size; D056951:Photoelectron Spectroscopy; D010973:Platelet Adhesiveness / Q000187:drug effects; D010976:Platelet Count; D011107:Polylysine / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D020112:Rhodamine 123 / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*; D014867:Water / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D042461:Vascular Endothelial Growth Factor A; D014867:Water; D020112:Rhodamine 123; D011107:Polylysine; D006493:Heparin; D004298:Dopamine
keywords: VEGF; dopamine; endothelialization; heparin; titanium