title: Alteration in chondroitin sulfate proteoglycan expression at the epicenter of spinal cord is associated with the loss of behavioral function in Tiptoe walking Yoshimura mice.
abstract: The objective of this study was to explore the correlation between the alteration in chondroitin sulfate proteoglycan (CSPG) expression at the epicenter of spinal cord and the loss of behavioral function in tiptoe walking Yoshimura mice. The tiptoe walking Yoshimura mice (twy) and Institute of Cancer Research (ICR) mice, aged 20 and 26 weeks, were used in the present study. The behavior assessment, micro-computed tomography and immunofluorescent staining were performed. The compressed spinal cord was histologically analyzed. The results showed that the expression of CSPG was statistically higher at the compressed spinal cord for twy mice compared with that at the normal spinal cord for ICR mice. At the 26th week, a large ossification block at the posterior longitudinal ligament of C1-3 was obviously observed at the micro-CT image We observed the BMS Score was significantly correlated with the expression of glial fibrillary acidic protein, CSPG and hyaluronan (P < 0.05). These findings suggest that compression injury induces the higher CSPG expression at the compressed spinal cord in the twy mice. Furthermore, the alteration in CSPG expression at the epicenter of spinal cord is associated with the loss of behavioral function in twy mice.
authors.affiliation: Department of Orthopedics, Peking University Third Hospital, 49 North Garden Rd, Haidian District, Beijing, 100191, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Rong
authors.forename: Jia
authors.initials: J
authors.lastname: Lv
authors.forename: Feng
authors.initials: F
authors.lastname: Wei
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D001522:Behavior, Animal; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism*; D017931:DNA Primers; D051379:Mice; D008813:Mice, Inbred ICR; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013116:Spinal Cord / Q000378:metabolism*
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D017931:DNA Primers