title: Targeting the raft-associated Akt signaling in hepatocellular carcinoma.
abstract: Caveolin-1 and flotillin-1 are considered as markers of lipid rafts which can be regarded as sorting platforms for targeted transport of transmembrane proteins and are involved in fundamental cellular events such as signal transduction, cell adhesion, lipid/protein sorting, and human cancer. We addressed caveolin-1 and flotillin-1 expression in 90 human hepatocellular carcinoma (HCC) and adjacent noncancerous tissues (ANT) samples by SDS-PAGE and immunoblotting with specific antibodies. Significant caveolin-1 and flotillin-1 overexpression was found in HCC tissues compared to ANT and was confirmed by immunohistochemistry. Raft-associated Akt signaling pathway components involved in the regulation of cell survival were altered by western blotting in HCC microdomain-enriched subcellular fractions purified from paired HCC and ANT samples. Our results demonstrated that the activity of raft-associated but not total membrane Akt determines its cellular functions. Lipid rafts differ in different types of tissues, which allows for the possibility of tissue-type-specific targeting for cell survival.
authors.affiliation: Department of Pathology and Hepatology, Beijing 302 Hospital, 100 Xi Si Huan Middle Road, Fengtai District, Beijing 100039, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pathology and Hepatology, Beijing 302 Hospital, 100 Xi Si Huan Middle Road, Fengtai District, Beijing 100039, China.
authors.forename: Ji-Yun
authors.initials: JY
authors.lastname: Lv
authors.affiliation: Department of Pathology and Hepatology, Beijing 302 Hospital, 100 Xi Si Huan Middle Road, Fengtai District, Beijing 100039, China.
authors.forename: Jian-Fei
authors.initials: JF
authors.lastname: Shi
authors.affiliation: Department of Pathology and Hepatology, Beijing 302 Hospital, 100 Xi Si Huan Middle Road, Fengtai District, Beijing 100039, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Pathology and Hepatology, Beijing 302 Hospital, 100 Xi Si Huan Middle Road, Fengtai District, Beijing 100039, China.
authors.forename: Shu-Hong
authors.initials: SH
authors.lastname: Liu
authors.affiliation: First Hepatobiliary Surgery Center, Beijing 302 Hospital, 100 Xi Si Huan Middle Road, Fengtai District, Beijing 100039, China.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Li
authors.affiliation: Secondary Hepatobiliary Surgery Center, Beijing 302 Hospital, 100 Xi Si Huan Middle Road, Fengtai District, Beijing 100039, China.
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Wang
authors.affiliation: First Hepatobiliary Surgery Center, Beijing 302 Hospital, 100 Xi Si Huan Middle Road, Fengtai District, Beijing 100039, China.
authors.forename: Shao-Geng
authors.initials: SG
authors.lastname: Zhang
authors.affiliation: Secondary Hepatobiliary Surgery Center, Beijing 302 Hospital, 100 Xi Si Huan Middle Road, Fengtai District, Beijing 100039, China.
authors.forename: Zhen-Wen
authors.initials: ZW
authors.lastname: Liu
authors.affiliation: Department of Pathology and Hepatology, Beijing 302 Hospital, 100 Xi Si Huan Middle Road, Fengtai District, Beijing 100039, China.
authors.forename: Jin-Biao
authors.initials: JB
authors.lastname: Ding
authors.affiliation: Beijing Institute of Infectious Diseases, Beijing 302 Hospital, 100 Xi Si Huan Middle Road, Fengtai District, Beijing 100039, China.
authors.forename: Dong-Ping
authors.initials: DP
authors.lastname: Xu
authors.affiliation: Department of Pathology and Hepatology, Beijing 302 Hospital, 100 Xi Si Huan Middle Road, Fengtai District, Beijing 100039, China.
authors.forename: Jing-Min
authors.initials: JM
authors.lastname: Zhao
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D051242:Caveolin 1 / Q000032:analysis / Q000378:metabolism; D002470:Cell Survival / Q000502:physiology; D005260:Female; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008103:Liver Cirrhosis / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D021962:Membrane Microdomains / Q000378:metabolism*; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism; D008875:Middle Aged; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D051242:Caveolin 1; D008565:Membrane Proteins; C106354:flotillins; D051057:Proto-Oncogene Proteins c-akt