title: Methane production from wheat straw with anaerobic sludge by heme supplementation.
abstract: Wheat straw particles were directly used as substrate for batch anaerobic digestion with anaerobic sludge under 35°C to evaluate the effects of adding heme on methane production. When 1mg/l heme was added to the fermentation process with no agitated speed, a maximum cumulative methane production of 12227.8ml was obtained with cumulative methane yield of wheat straw was 257.4ml/g-TS (total solid), which was increased by 20.6% compared with 213.5ml/g-TS of no heme was added in the reactor. Meanwhile, oxido-reduction potential (ORP) level was decreased, the activity of coenzyme F420 was significantly improved and NADH/NAD(+) ratio were the highest than other experimental groups. These results suggest that heme-supplemented anaerobic sludge with no agitated speed may be providing a more reductive environment, which is a cost-effective method of anaerobic digestion from biomass waste to produce methane with less energy consuming.
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Laboratory for Agricultural Wastes Treatment and Recycling, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Science, Nanjing 210014, China.
authors.forename: Yonglan
authors.initials: Y
authors.lastname: Xi
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Laboratory for Agricultural Wastes Treatment and Recycling, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Science, Nanjing 210014, China. Electronic address: czhizhou@hotmail.com.
authors.forename: Zhizhou
authors.initials: Z
authors.lastname: Chang
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Laboratory for Agricultural Wastes Treatment and Recycling, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Science, Nanjing 210014, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Ye
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Laboratory for Agricultural Wastes Treatment and Recycling, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Science, Nanjing 210014, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xu
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Laboratory for Agricultural Wastes Treatment and Recycling, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Science, Nanjing 210014, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Du
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Laboratory for Agricultural Wastes Treatment and Recycling, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Science, Nanjing 210014, China.
authors.forename: Guangyin
authors.initials: G
authors.lastname: Chen
mesh_terms: D000693:Anaerobiosis / Q000187:drug effects; D061249:Batch Cell Culture Techniques; D001709:Biotechnology / Q000379:methods*; D005232:Fatty Acids, Volatile / Q000032:analysis; D005285:Fermentation / Q000187:drug effects; D006418:Heme / Q000494:pharmacology*; D008697:Methane / Q000096:biosynthesis*; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D012722:Sewage / Q000737:chemistry* / Q000382:microbiology; D013997:Time Factors; D014908:Triticum / Q000737:chemistry* / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D014866:Waste Products / Q000032:analysis*
chemical_list: D005232:Fatty Acids, Volatile; D012722:Sewage; D014866:Waste Products; D009243:NAD; D006418:Heme; D008697:Methane
keywords: Anaerobic digestion; Heme; Methane; NADH/NAD(+); Wheat straw