title: Polycyclic aromatic hydrocarbons (PAHs) in yogurt samples.
abstract: The concentrations and distributions of major polycyclic aromatic hydrocarbons (PAHs) were determined in 20 kinds of yogurt specimens collected from Italian supermarkets using reversed phase high-performance liquid chromatography equipped with fluorescence detection. The method was validated by determination of recovery percentages, precision (repeatability) and sensitivity (limits of detection) with yogurt samples fortified at 0.25, 0.5 and 1 µg/kg concentration levels. The recovery of 13 PAHs, with the exception of naphthalene and acenaphthene, ranged from 61% to 130% and from 60% to 97% at all the levels for yogurts with low (0.1%) and high (3.9%) fat content, respectively. The method is repeatable with relative standard deviation values <20% for all analytes. The results obtained demonstrate that acenaphthene, fluorantene, phenanthrene, anthracene, fluoranthene and pyrene were found in all samples with a similar distribution, but different content when yogurts with low and high fats were compared.
authors.affiliation: a Department of Chemistry , Sapienza University of Rome , Rome , Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Battisti
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Girelli
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Tarola
mesh_terms: D000073:Acenaphthenes / Q000032:analysis; D053000:Analytic Sample Preparation Methods; D001644:Bifidobacterium / Q000254:growth & development / Q000378:metabolism; D002274:Carcinogens, Environmental / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D043302:Cultured Milk Products / Q000737:chemistry / Q000191:economics / Q000382:microbiology / Q000592:standards; D018752:Diet, Fat-Restricted; D005062:European Union; D005449:Fluorenes / Q000032:analysis; D005506:Food Contamination; D005513:Food Inspection / Q000379:methods*; D017408:Guidelines as Topic; D006358:Hot Temperature; D006868:Hydrolysis; D007558:Italy; D057230:Limit of Detection; D010616:Phenanthrenes / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D015014:Yogurt / Q000032:analysis* / Q000191:economics / Q000592:standards
chemical_list: D000073:Acenaphthenes; D002274:Carcinogens, Environmental; D005449:Fluorenes; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; C041509:fluorene; C031181:phenanthrene; C042552:acenaphthene
keywords: PAHs; RP-HPLC; fluorescence detection; yogurt