title: Chemical alteration by tooth bleaching of human salivary proteins that infiltrated subsurface enamel lesions--experimental study with bovine lesion model systems.
abstract: Salivary macromolecules infiltrate white and brown spot enamel lesions and adsorb onto hydroxyapatite. Calcium-binding salivary proteins such as statherin hinder remineralization of these lesions. We assessed whether bleaching agents can remove salivary components that have infiltrated and bound to experimental subsurface lesions in bovine enamel prepared by immersing specimens in acid and then human saliva. Transversal microradiography showed that such demineralized lesions mimicked incipient carious lesions. Bound proteins to the experimental and untreated control specimens were eluted in a stepwise manner with phosphatebuffered saline, 0.4 M phosphate buffer, and 1 M HCl. SDS-PAGE of dialyzed extracts showed that specific salivary proteins bound to the lesions, while virtually no protein bands were detected if the specimens were bleached. Western blotting showed that even statherin, which was more firmly bound than other proteins, was removed. In-office bleaching agent may be useful in treating enamel lesions for removing proteins bound to these lesions.
authors.affiliation: Department of Cariology and Restorative Dentistry, Graduate School of Dentistry, Kanagawa Dental University.
authors.forename: Junko
authors.initials: J
authors.lastname: Iizuka
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Mukai
authors.forename: Motoe
authors.initials: M
authors.lastname: Taniguchi
authors.forename: Yuko
authors.initials: Y
authors.lastname: Mikuni-Takagaki
authors.forename: Jacob Martien
authors.initials: JM
authors.lastname: Ten Cate
authors.forename: Toshio
authors.initials: T
authors.lastname: Teranaka
mesh_terms: D000818:Animals; D002417:Cattle; D003743:Dental Enamel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008954:Models, Biological; D012471:Salivary Proteins and Peptides / Q000378:metabolism*; D014073:Tooth Bleaching
chemical_list: D012471:Salivary Proteins and Peptides