title: Highly selective and sensitive near-infrared-fluorescent probes for the detection of cellular hydrogen sulfide and the imaging of H2S in mice.
abstract: Herein, we report the development of two fluorescent probes for the highly selective and sensitive detection of H2S. The probes take advantage of a Cu(II)-cyclen complex, which acts as a reaction center for H2S and as a quencher of BODIPY (boron-dipyrromethene)-based fluorophores with emissions at 765 and 680 nm, respectively. These non-fluorescent probes could only be turned on by the addition of H2 S, and not by other potentially interfering biomolecules, including reactive oxygen species, cysteine, and glutathione. In a chemical system, both probes detected H2S with a detection limit of 80 nM. The probes were successfully used for the endogenous detection of H2S in HEK 293 cells, for measuring the H2S-release activity of dietary organosulfides in MCF-7 cells, and for the in vivo imaging of H2S in mice.
authors.affiliation: Food Science and Technology Programme, Department of Chemistry, National University of Singapore, Science Drive 3, 117543 (Singapore).
authors.forename: Haixia
authors.initials: H
authors.lastname: Wu
authors.forename: Saarangan
authors.initials: S
authors.lastname: Krishnakumar
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.forename: Dong
authors.initials: D
authors.lastname: Liang
authors.forename: Hongyi
authors.initials: H
authors.lastname: Qi
authors.forename: Zheng-Wei
authors.initials: ZW
authors.lastname: Lee
authors.forename: Lih-Wen
authors.initials: LW
authors.lastname: Deng
authors.forename: Dejian
authors.initials: D
authors.lastname: Huang
mesh_terms: D000818:Animals; D003300:Copper / Q000008:administration & dosage / Q000737:chemistry; D000080027:Cyclams; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry*; D057809:HEK293 Cells; D006571:Heterocyclic Compounds / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis*; D061986:MCF-7 Cells; D051379:Mice; D008813:Mice, Inbred ICR; D057054:Molecular Imaging; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D000080027:Cyclams; D005456:Fluorescent Dyes; D006571:Heterocyclic Compounds; D009942:Organometallic Compounds; D003300:Copper; C038072:cyclen; D006862:Hydrogen Sulfide
keywords: copper; fluorescence; hydrogen sulfide; imaging agents; probes