title: Fast Step Transition and State Identification (STaSI) for Discrete Single-Molecule Data Analysis.
abstract: We introduce a step transition and state identification (STaSI) method for piecewise constant single-molecule data with a newly derived minimum description length equation as the objective function. We detect the step transitions using the Student'sttest and group the segments into states by hierarchical clustering. The optimum number of states is determined based on the minimum description length equation. This method provides comprehensive, objective analysis of multiple traces requiring few user inputs about the underlying physical models and is faster and more precise in determining the number of states than established and cutting-edge methods for single-molecule data analysis. Perhaps most importantly, the method does not require either time-tagged photon counting or photon counting in general and thus can be applied to a broad range of experimental setups and analytes.
authors.affiliation: Department of Chemistry, Rice University , MS 60, Houston, Texas 77251-1892, United States.
authors.forename: Bo
authors.initials: B
authors.lastname: Shuang
authors.affiliation: Department of Chemistry, Rice University , MS 60, Houston, Texas 77251-1892, United States.
authors.forename: David
authors.initials: D
authors.lastname: Cooper
authors.affiliation: Molecule & Life Nonlinear Sciences Laboratory, Research Institute for Electronic Science, Hokkaido University , Sapporo 001-0020, Japan.
authors.forename: J Nick
authors.initials: JN
authors.lastname: Taylor
authors.affiliation: Department of Chemistry, Rice University , MS 60, Houston, Texas 77251-1892, United States.
authors.forename: Lydia
authors.initials: L
authors.lastname: Kisley
authors.affiliation: Department of Chemistry, Rice University , MS 60, Houston, Texas 77251-1892, United States.
authors.forename: Jixin
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Electrical and Computer Engineering, Rice Quantum Institute, Rice University , MS 60, Houston, Texas 77251-1892, United States.
authors.forename: Wenxiao
authors.initials: W
authors.lastname: Wang
authors.affiliation: Molecule & Life Nonlinear Sciences Laboratory, Research Institute for Electronic Science, Hokkaido University , Sapporo 001-0020, Japan.
authors.forename: Chun Biu
authors.initials: CB
authors.lastname: Li
authors.affiliation: Molecule & Life Nonlinear Sciences Laboratory, Research Institute for Electronic Science, Hokkaido University , Sapporo 001-0020, Japan.
authors.forename: Tamiki
authors.initials: T
authors.lastname: Komatsuzaki
authors.affiliation: Department of Chemistry, Rice University , MS 60, Houston, Texas 77251-1892, United States ; Department of Electrical and Computer Engineering, Rice Quantum Institute, Rice University , MS 60, Houston, Texas 77251-1892, United States.
authors.forename: Christy F
authors.initials: CF
authors.lastname: Landes