title: Combination of single-photon emission computed tomography and magnetic resonance imaging to track 111in-oxine-labeled human mesenchymal stem cells in neuroblastoma-bearing mice.
abstract: Homing is an inherent, complex, multistep process performed by cells such as human bone marrow mesenchymal stem cells (hMSCs) to travel from a distant location to inflamed or damaged tissue and tumors. This ability of hMSCs has been exploited as a tumor-targeting strategy in cell-based cancer therapy. The purpose of this study was to investigate the applicability of 111In-oxine for tracking hMSCs in vivo by combining single-photon emission computed tomography (SPECT) and magnetic resonance imaging (MRI). 111In-labeled hMSCs (106 cells) were infused intraperitoneally in neuroblastoma-bearing mice, whereas a control group received a dose of free 111In-oxine. SPECT and MRI studies were performed 24 and 48 hours afterwards. Initially, the images showed similar activity in the abdomen in both controls and hMSC-injected animals. In general, abdominal activity decreases at 48 hours. hMSC-injected animals showed increased uptake in the tumor area at 48 hours, whereas the control group showed a low level of activity at 24 hours, which decreased at 48 hours. In conclusion, tracking 111In-labeled hMSCs combining SPECT and MRI is feasible and may be transferable to clinical research. The multimodal combination is essential to ensure appropriate interpretation of the images.
authors.forename: Lorena
authors.initials: L
authors.lastname: Cussó
authors.forename: Isabel
authors.initials: I
authors.lastname: Mirones
authors.forename: Santiago
authors.initials: S
authors.lastname: Peña-Zalbidea
authors.forename: Verónica
authors.initials: V
authors.lastname: García-Vázquez
authors.forename: Javier
authors.initials: J
authors.lastname: García-Castro
authors.forename: Manuel
authors.initials: M
authors.lastname: Desco
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000000981:diagnostic imaging*; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D009447:Neuroblastoma / Q000000981:diagnostic imaging* / Q000473:pathology; D009942:Organometallic Compounds / Q000493:pharmacokinetics*; D015125:Oxyquinoline / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods
chemical_list: D009942:Organometallic Compounds; C032950:indium oxine; D015125:Oxyquinoline