title: In vivo evaluation of analgesic, anti-inflammatory, and neuropharmacological activities of the chemical constituent from Nepeta clarkei.
abstract: The plant species of genus Nepeta are used to treat various human diseases and for ornamental purposes as well. Nepethalate B (1) was isolated as a result of phytochemical investigations of Nepeta clarkei and was subjected in the present study for investigation of analgesic, anti-inflammatory, and central nervous system (CNS) depressant activities. The percentage inhibition in phase I of the acetic acid induced writhing test of compound 1 (dose of 0.1, 0.2 and 0.4 mg/kg of body weight) was 53.3, 58.0 and 86.7% respectively. These results were found significantly higher (P < 0.01) as compared to the negative control. Moreover, the percentage inhibitions of three phases for 0.1, 0.2 and 0.4 mg/kg were higher than the values obtained for Aspirin (positive control). In formalin test, the percentage pain inhibition between 0 and 5 min (early phase) was 68.0, 25.5, and 75.5% for 0.1, 0.2 and 0.4 mg/kg intra-peritoneal doses of compound 1 respectively. In case of late phase (20-30 min) it was 63.0, 66.7 and 48.1%, respectively. In comparison to aspirin, overall percentage inhibition of compound 1 was significantly higher in early and late phases. Interestingly, at all doses compound 1 showed more potent anti-inflammatory effects in terms of intensity and duration as compared to aspirin. The gross behavioral study of nepethalate B (1) was also carried out and the results revealed that it exhibited CNS depression in the mice and showed a prominent decrease in locomotor activity.
authors.affiliation: Department of Biological Sciences and Chemistry, College of Arts and Sciences, University of Nizwa, Nizwa, 616, Sultanate of Oman, javidhej@yahoo.com.
authors.forename: Javid
authors.initials: J
authors.lastname: Hussain
authors.forename: Najeeb Ur
authors.initials: NU
authors.lastname: Rehman
authors.forename: Ahmed
authors.initials: A
authors.lastname: Al-Harrasi
authors.forename: Abdul Latif
authors.initials: AL
authors.lastname: Khan
authors.forename: Tania Shamim
authors.initials: TS
authors.lastname: Rizvi
authors.forename: Faryal Vali
authors.initials: FV
authors.lastname: Mohammad
authors.lastname: Mehjabeen
authors.forename: Liaqat
authors.initials: L
authors.lastname: Ali
mesh_terms: D019342:Acetic Acid; D000700:Analgesics; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001241:Aspirin / Q000627:therapeutic use; D001522:Behavior, Animal / Q000187:drug effects; D002492:Central Nervous System Depressants / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005557:Formaldehyde; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D027521:Nepeta / Q000737:chemistry*; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy / Q000523:psychology; D010795:Phthalic Acids / Q000494:pharmacology*
chemical_list: D000700:Analgesics; D000894:Anti-Inflammatory Agents, Non-Steroidal; D002492:Central Nervous System Depressants; D010795:Phthalic Acids; C000605641:nepethalate B; D005557:Formaldehyde; D019342:Acetic Acid; D001241:Aspirin