title: A single immunization with modified vaccinia virus Ankara-based influenza virus H7 vaccine affords protection in the influenza A(H7N9) pneumonia ferret model.
abstract: Since the first reports in early 2013, >440 human cases of infection with avian influenza A(H7N9) have been reported including 122 fatalities. After the isolation of the first A(H7N9) viruses, the nucleotide sequences became publically available. Based on the coding sequence of the influenza virus A/Shanghai/2/2013 hemagglutinin gene, a codon-optimized gene was synthesized and cloned into a recombinant modified vaccinia virus Ankara (MVA). This MVA-H7-Sh2 viral vector was used to immunize ferrets and proved to be immunogenic, even after a single immunization. Subsequently, ferrets were challenged with influenza virus A/Anhui/1/2013 via the intratracheal route. Unprotected animals that were mock vaccinated or received empty vector developed interstitial pneumonia characterized by a marked alveolitis, accompanied by loss of appetite, weight loss, and heavy breathing. In contrast, animals vaccinated with MVA-H7-Sh2 were protected from severe disease.
authors.affiliation: Department of Viroscience, Erasmus Medical Center.
authors.forename: Joost H C M
authors.initials: JH
authors.lastname: Kreijtz
authors.affiliation: Department of Viroscience, Erasmus Medical Center.
authors.forename: Lidewij C M
authors.initials: LC
authors.lastname: Wiersma
authors.affiliation: Department of Viroscience, Erasmus Medical Center.
authors.forename: Heidi L M
authors.initials: HL
authors.lastname: De Gruyter
authors.affiliation: Department of Viroscience, Erasmus Medical Center.
authors.forename: Stella E
authors.initials: SE
authors.lastname: Vogelzang-van Trierum
authors.affiliation: Viroclinics Biosciences, Rotterdam, the Netherlands.
authors.forename: Geert
authors.initials: G
authors.lastname: van Amerongen
authors.affiliation: Viroclinics Biosciences, Rotterdam, the Netherlands.
authors.forename: Koert J
authors.initials: KJ
authors.lastname: Stittelaar
authors.affiliation: Department of Viroscience, Erasmus Medical Center Viroclinics Biosciences, Rotterdam, the Netherlands Institute for Infectious Diseases and Zoonoses, LMU University of Munich German Center for Infection Research, Braunschweig, Germany.
authors.forename: Ron A M
authors.initials: RA
authors.lastname: Fouchier
authors.affiliation: Department of Viroscience, Erasmus Medical Center.
authors.forename: Albert D M E
authors.initials: AD
authors.lastname: Osterhaus
authors.affiliation: Institute for Infectious Diseases and Zoonoses, LMU University of Munich German Center for Infection Research, Braunschweig, Germany.
authors.forename: Gerd
authors.initials: G
authors.lastname: Sutter
authors.affiliation: Department of Viroscience, Erasmus Medical Center.
authors.forename: Guus F
authors.initials: GF
authors.lastname: Rimmelzwaan
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004337:Drug Carriers; D005260:Female; D005289:Ferrets; D005822:Genetic Vectors; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D064766:Influenza A Virus, H7N9 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D017563:Lung Diseases, Interstitial / Q000473:pathology / Q000517:prevention & control; D009976:Orthomyxoviridae Infections / Q000473:pathology / Q000517:prevention & control*; D016896:Treatment Outcome; D014611:Vaccination / Q000379:methods; D014616:Vaccinia virus / Q000235:genetics*
chemical_list: D004337:Drug Carriers; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; C109963:hemagglutinin, avian influenza A virus
keywords: Influenza A(H7N9) virus; Modified Vaccinia virus Ankara; vaccine