title: S100A6 is secreted from Wharton's jelly mesenchymal stem cells and interacts with integrin β1.
abstract: S100A6 is a calcium binding protein belonging to the S100 family. In this work we examined the function of extracellular S100A6. Using mesenchymal stem cells isolated from Wharton's jelly of the umbilical cord (WJMS cells) we have shown that S100A6 is secreted by these cells, and when added to the medium, increases their adhesion and inhibits proliferation. The search for a potential target/receptor of S100A6 in the membrane fraction of WJMS cells allowed us to identify some proteins, among them integrin β1, which interacts with S100A6 in a calcium dependent manner. The interaction between S100A6 and integrin β1, was then confirmed by ELISA using purified proteins. Applying specific antibodies against integrin β1 reversed the effect on cell adhesion and proliferation observed in the presence of S100A6 which indicates that S100A6 exerts its function due to interaction with integrin β1. Since the data show the influence of extracellular S100A6 on cells isolated from Wharton's jelly, our results might help to establish molecular mechanisms leading to some pathologies characteristic for this tissue.
authors.affiliation: Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Jurewicz
authors.affiliation: Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Góral
authors.affiliation: Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland. Electronic address: a.filipek@nencki.gov.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Filipek
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D018797:Cell Cycle Proteins / Q000378:metabolism* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D015151:Immunoblotting; D019012:Integrin beta1 / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D011485:Protein Binding; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins / Q000378:metabolism* / Q000494:pharmacology; D059631:Wharton Jelly / Q000166:cytology*
chemical_list: D018797:Cell Cycle Proteins; D019012:Integrin beta1; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C055351:S100A6 protein, human
keywords: Cell adhesion; Integrin β1; S100A6; Wharton's jelly mesenchymal stem cells