title: First genotyping of Giardia duodenalis and prevalence of enteroparasites in children from Tetouan (Morocco).
abstract: Intestinal parasites are common in the Moroccan population. Enteroparasites in children from four schools in urban and rural areas of Tetouan (Morocco) were studied to treat these children and to design prevention and control programs. A total of 673 children were examined. The prevalence of parasitized children was 51%. The average number of enteroparasites was half in urban areas than in rural areas. Multiple parasitism appeared in 30% of the samples presenting two, three, or four parasites. The most prevalent parasite was Blastocystis hominis (64%). Giardia duodenalis was the most frequent pathogen, with an overall prevalence of 20% (24% in rural areas and 16% in urban areas). Other pathogenic enteroparasites were Cyclospora cayetanensis (5% in rural and urban areas), Iodamoeba butschlii, Hymenolepis spp., Trichuris trichiura and Enterobius vermicularis, with prevalence lower than 2%. In this work, G. duodenalis genotypes were molecularly characterized by a study of the glutamate dehydrogenase (gdh) and 18S rRNA genes. This is the first study of molecular characterization of G. duodenalis in Moroccan children, and the sequence analysis revealed both Assemblage A (AII) and Assemblage B (BIII, BIV), with the predominance of Assemblage BIV (73%).
authors.affiliation: Département de Biologie, Faculté des Sciences, Université Abdelmalek Essaâdi, Tétouan, Morocco.
authors.forename: Chadia
authors.initials: C
authors.lastname: El Fatni
authors.affiliation: Departamento de Parasitología, Facultad de Ciencias, Universidad de Granada, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Olmo
authors.affiliation: Département de Biologie, Faculté des Sciences, Université Abdelmalek Essaâdi, Tétouan, Morocco.
authors.forename: Hoummad
authors.initials: H
authors.lastname: El Fatni
authors.affiliation: Departamento de Estadística, Facultad de Ciencias, Universidad de Granada, Spain.
authors.forename: Desiré
authors.initials: D
authors.lastname: Romero
authors.affiliation: Departamento de Parasitología, Facultad de Ciencias, Universidad de Granada, Spain.
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Rosales
mesh_terms: D000293:Adolescent; D000818:Animals; D000829:Animals, Domestic / Q000469:parasitology; D000835:Animals, Wild / Q000469:parasitology; D002648:Child; D002675:Child, Preschool; D004197:Disease Reservoirs / Q000469:parasitology; D005260:Female; D017125:Genes, Protozoan; D005838:Genotype; D016829:Giardia lamblia / Q000235:genetics* / Q000302:isolation & purification; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology*; D005969:Glutamate Dehydrogenase / Q000235:genetics; D006798:Housing; D006801:Humans; D007411:Intestinal Diseases, Parasitic / Q000453:epidemiology* / Q000469:parasitology; D008297:Male; D009018:Morocco / Q000453:epidemiology; D015995:Prevalence; D015800:Protozoan Proteins / Q000235:genetics; D016053:RNA, Protozoan / Q000235:genetics; D012424:Rural Population; D012959:Socioeconomic Factors; D014505:Urban Population
chemical_list: D015800:Protozoan Proteins; D016053:RNA, Protozoan; D005969:Glutamate Dehydrogenase