title: Saccharothrix carnea sp. nov., an actinobacterium isolated from soil.
abstract: A novel actinobacterium, designated strain NEAU-yn17(T), was isolated from a soil sample collected at the wastewater discharge site of a pesticide factory in Harbin, northern China, and characterized using a polyphasic approach. Morphological and chemotaxonomic properties of strain NEAU-yn17(T) were consistent with the description of the genus Saccharothrix, such as the spore arrangement, the diamino acid of the peptidoglycan, the whole-cell hydrolysates, the predominant menaquinone and the phospholipid profile. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strain NEAU-yn17(T) should also be classified in the genus Saccharothrix, with Saccharothrix saharensis DSM 45456(T) (99.52 % sequence similarity) and Saccharothrix xinjiangensis JCM 12329(T) (99.04 %) as the nearest phylogenetic relatives. A combination of DNA-DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-yn17(T) can be distinguished from its closest relatives. Therefore, strain NEAU-yn17(T) represents a novel species of the genus Saccharothrix, for which the name Saccharothrix carnea sp. nov. is proposed. The type strain is NEAU-yn17(T) ( = CGMCC 4.7097(T) = DSM 45878(T)).
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Chongxi
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Guan
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Shurui
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Hairong
authors.initials: H
authors.lastname: He
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.|Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Xiang
authors.affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
authors.forename: Xiangjing
authors.initials: X
authors.lastname: Wang
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D007220:Industrial Waste; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010575:Pesticides; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010860:Pigments, Biological / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000737:chemistry; D062065:Wastewater
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D007220:Industrial Waste; D010457:Peptidoglycan; D010575:Pesticides; D010743:Phospholipids; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D062065:Wastewater