title: Molecular characterization of the Jatropha curcas JcR1MYB1 gene encoding a putative R1-MYB transcription factor.
abstract: The cDNA encoding the R1-MYB transcription factor, designated as JcR1MYB1, was isolated from Jatropha curcas using rapid amplification of cDNA ends. JcR1MYB1 contains a 951 bp open reading frame that encodes 316 amino acids. The deduced JcR1MYB1 protein was predicted to possess the conserved, 56-amino acid-long DNA-binding domain, which consists of a single helix-turn-helix module and usually occurs in R1-MYBs. JcR1MYB1 is a member of the R1-MYB transcription factor subfamily. A subcellular localization study confirmed the nuclear localization of JcR1MYB1. Expression analysis showed that JcR1MYB1 transcripts accumulated in various examined tissues, with high expression levels in the root and low levels in the stem. JcR1MYB1 transcription was up-regulated by polyethylene glycol, NaCl, and cold treatments, as well as by abscisic acid, jasmonic acid, and ethylene treatment. Analysis of transgenic tobacco plants over-expressing JcR1MYB1 indicates an inportant function for this gene in salt stress.
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops , Ministry of Agriculture , Institute of Tropical Bioscience and Biotechnology , Chinese Academy of Tropical Agricultural Sciences , Haikou , China .
authors.forename: Hui-Liang
authors.initials: HL
authors.lastname: Li
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops , Ministry of Agriculture , Institute of Tropical Bioscience and Biotechnology , Chinese Academy of Tropical Agricultural Sciences , Haikou , China .
authors.forename: Dong
authors.initials: D
authors.lastname: Guo
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops , Ministry of Agriculture , Institute of Tropical Bioscience and Biotechnology , Chinese Academy of Tropical Agricultural Sciences , Haikou , China .
authors.forename: Shi-Qing
authors.initials: SQ
authors.lastname: Peng
keywords: Jatropha curcas; R1-MYB transcription factor; abiotic stress; gene expression