title: Laboratory-size three-dimensional x-ray microscope with Wolter type I mirror optics and an electron-impact water window x-ray source.
abstract: We constructed a laboratory-size three-dimensional water window x-ray microscope that combines wide-field transmission x-ray microscopy with tomographic reconstruction techniques, and observed bio-medical samples to evaluate its applicability to life science research fields. It consists of a condenser and an objective grazing incidence Wolter type I mirror, an electron-impact type oxygen Kα x-ray source, and a back-illuminated CCD for x-ray imaging. A spatial resolution limit of around 1.0 line pairs per micrometer was obtained for two-dimensional transmission images, and 1-μm scale three-dimensional fine structures were resolved.
authors.affiliation: Hamamatsu Photonics K.K., 5000 Hirakuchi, Hamakita-ku, Hamamatsu-City, 434-8601, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Ohsuka
authors.affiliation: Hamamatsu Photonics K.K., 5000 Hirakuchi, Hamakita-ku, Hamamatsu-City, 434-8601, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Ohba
authors.affiliation: Hamamatsu Photonics K.K., 5000 Hirakuchi, Hamakita-ku, Hamamatsu-City, 434-8601, Japan.
authors.forename: Shinobu
authors.initials: S
authors.lastname: Onoda
authors.affiliation: Hamamatsu Photonics K.K., 5000 Hirakuchi, Hamakita-ku, Hamamatsu-City, 434-8601, Japan.
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Nakamoto
authors.affiliation: Hamamatsu Photonics K.K., 5000 Hirakuchi, Hamakita-ku, Hamamatsu-City, 434-8601, Japan.
authors.forename: Tomoyasu
authors.initials: T
authors.lastname: Nakano
authors.affiliation: Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
authors.forename: Motosuke
authors.initials: M
authors.lastname: Miyoshi
authors.affiliation: Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
authors.forename: Keita
authors.initials: K
authors.lastname: Soda
authors.affiliation: Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Hamakubo
mesh_terms: D000818:Animals; D004583:Electrons; D021621:Imaging, Three-Dimensional / Q000295:instrumentation*; D007668:Kidney / Q000166:cytology; D007753:Laboratories; D051379:Mice; D008853:Microscopy / Q000295:instrumentation*; D014867:Water; D014965:X-Rays
chemical_list: D014867:Water