title: Production, purification and application of polysaccharide-based bioflocculant by Paenibacillus mucilaginosus.
abstract: The production and purification of polysaccharide-based bioflocculants (PSBs) by Paenibacillus mucilaginosus GIM1.16 in metal ion-supplemented medium and basal medium were evaluated. Three purified PSB1-1, PSB2-1 and PSB3-1 possessed different monosaccharide composition and their molecular weights were 2.53 × 10(6), 7.77 × 10(6) and 13.2 × 10(6)Da, respectively. FT-IR spectrometry indicated the presence of hydroxyl, carboxyl and phosphate groups in the three samples. Scanning electron microscopy showed that they had linear structure. The potential of these PSBs on wastewater treatment was evaluated. Among them, PSB1-1 exhibited the best performance, as it had high flocculating activities (above 94%) at 0.5-4 mg/L and could achieve high flocculating activities (above 97%) in the kaolin suspensions of pH 3-9. PSB1-1 was the key factor that might explain the enhanced flocculating activity of the supernatant from metal ion-supplemented medium. The performance of PSB1-1 on industrial wastewater was also satisfactory. PSB1-1 might be a good candidate as bioflocculant.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, 510006 Guangzhou, China.
authors.forename: Jiayi
authors.initials: J
authors.lastname: Tang
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, 510641 Guangzhou, China.
authors.forename: Suijian
authors.initials: S
authors.lastname: Qi
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, 510006 Guangzhou, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Li
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, 510006 Guangzhou, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: An
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, 510006 Guangzhou, China.
authors.forename: Mingquan
authors.initials: M
authors.lastname: Xie
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, 510006 Guangzhou, China. Electronic address: yangbo@scut.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, 510641 Guangzhou, China. Electronic address: yonghw@scut.edu.cn.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D005427:Flocculation; D056507:Paenibacillus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D062065:Wastewater / Q000737:chemistry*; D018508:Water Purification
chemical_list: D011134:Polysaccharides; D062065:Wastewater
keywords: Bioflocculants; Flocculating activity; Metal ion; Paenibacillus mucilaginosus; Purification