title: A study of the interleukin-1 gene cluster polymorphisms and inflammatory external root resorption in replanted permanent teeth.
abstract: OBJECTIVE
  To investigate whether single nucleotide polymorphisms (SNPs) within the interleukin-1 gene cluster (IL1) are associated with the occurrence and severity of inflammatory external root resorption (IERR) after replantation of avulsed permanent teeth.
  
  
  METHODS
  Indexes of IERR were radiographically assessed in 182 mature replanted permanent teeth from 146 patients at the onset of endodontic therapy. DNA was extracted from buccal mucosa cells and genotyped using TaqMan probes-based assays for the SNPs IL1A -889C/T (rs 180058), IL1B +3954C/T (rs1143634) and IL1RN +2018C/T (rs419598). Teeth were grouped into two categories: IERR absent to mild (indexes ≤ 4) and moderate to severe IERR (indexes > 4). Genetic variations in the IL1 gene cluster were tested for their effect on the occurrence and extension of IERR using the GEE model (generalized estimation equation). Patient's age at the moment of injury, timing of pulpectomy, extra-alveolar period and storage condition of the avulsed teeth was included as possible confounders.
  
  
  RESULTS
  No association was found between SNPs IL1A -889C/T, IL1B +3954C/T (rs1143634) and IL1RN +2018C/T (rs419598) and IERR indexes. Timing of pulpectomy (OR 3.5 IC 95% 2.0-6.2 P < 0.001) and patient's age at the moment of trauma (OR 0.29 IC 95% 0.12-0.67 P = 0.004) significantly affected the risk of developing severe IERR.
  
  
  CONCLUSIONS
  While timing of pulpectomy and patient's age at the moment of trauma were confirmed as important risk factors, SNPs within the IL1 gene cluster did not affect the susceptibility for IERR after replantation of permanent teeth.
authors.affiliation: Dental Trauma Programme, Department of Restorative Dentistry, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: J V
authors.initials: JV
authors.lastname: Bastos
authors.affiliation: Dental Trauma Programme, Department of Restorative Dentistry, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: M I S
authors.initials: MI
authors.lastname: Côrtes
authors.affiliation: Faculty of Health and Biological Sciences, Newton Paiva University Center, Belo Horizonte, MG, Brazil.
authors.forename: J F C
authors.initials: JF
authors.lastname: Silva
authors.affiliation: Department of Pediatrics, School of Medicine, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: E M A
authors.initials: EM
authors.lastname: Goulart
authors.affiliation: Department of Statistics, Institute of Exact Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: E A
authors.initials: EA
authors.lastname: Colosimo
authors.affiliation: Department of Oral Surgery and Pathology, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: R S
authors.initials: RS
authors.lastname: Gomez
authors.affiliation: Laboratory of Cell-cell Interactions, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: W O
authors.initials: WO
authors.lastname: Dutra
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D001938:Brazil; D002648:Child; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D008297:Male; D005810:Multigene Family; D020641:Polymorphism, Single Nucleotide; D011670:Pulpectomy; D012307:Risk Factors; D012391:Root Resorption / Q000209:etiology / Q000235:genetics*; D013997:Time Factors; D014084:Tooth Avulsion / Q000628:therapy; D014090:Tooth Replantation / Q000379:methods*
chemical_list: D007375:Interleukin-1
keywords: inflammatory external root resorption; interleukin-1; interleukin-1 receptor antagonist; single nucleotide polymorphism; tooth avulsion; tooth replantation