title: Ultra-sensitive immunoassay biosensors using hybrid plasmonic-biosilica nanostructured materials.
abstract: We experimentally demonstrate an ultra-sensitive immunoassay biosensor using diatom biosilica with self-assembled plasmonic nanoparticles. As the nature-created photonic crystal structures, diatoms have been adopted to enhance surface plasmon resonances of metal nanoparticles on the surfaces of diatom frustules and to increase the sensitivity of surface-enhanced Raman scattering (SERS). In this study, a sandwich SERS immunoassay is developed based on the hybrid plasmonic-biosilica nanostructured materials that are functionalized with goat anti-mouse IgG. Our experimental results show that diatom frustules improve the detection limit of mouse IgG to 10 pg/mL, which is ˜100× better than conventional colloidal SERS sensors on flat glass. Ultra-sensitive immunoassay biosensor using diatom biosilica with self-assembled plasmonic nanoparticles.
authors.affiliation: School of Electrical Engineering and Computer Science, Oregon State University, Corvallis, 97331, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: School of Chemical, Biological & Environmental Engineering, Oregon State University, Corvallis, 97331, USA.
authors.forename: Le
authors.initials: L
authors.lastname: Zhen
authors.affiliation: School of Electrical Engineering and Computer Science, Oregon State University, Corvallis, 97331, USA.
authors.forename: Fanghui
authors.initials: F
authors.lastname: Ren
authors.affiliation: School of Chemical, Biological & Environmental Engineering, Oregon State University, Corvallis, 97331, USA.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Campbell
authors.affiliation: School of Chemical, Biological & Environmental Engineering, Oregon State University, Corvallis, 97331, USA. rorrergl@engr.orst.edu.
authors.forename: Gregory L
authors.initials: GL
authors.lastname: Rorrer
authors.affiliation: School of Electrical Engineering and Computer Science, Oregon State University, Corvallis, 97331, USA. wang@eecs.oregonstate.edu.
authors.forename: Alan X
authors.initials: AX
authors.lastname: Wang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D017377:Diatoms / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry*; D017785:Photons; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D012822:Silicon Dioxide
keywords: diatom biosilica; immunoassay; photonic crystal; surface plasmon; surface-enhanced Raman scattering