title: Live cell imaging of intracellular Salmonella enterica.
abstract: During the intracellular phase of the pathogenic lifestyle, Salmonella enterica massively alters the endosomal system of its host cells. Two hallmarks are the remodeling of phagosomes into the Salmonella-containing vacuole (SCV) as a replicative niche, and the formation of tubular structures, such as Salmonella-induced filaments (SIFs). To study the dynamics and the fate of these Salmonella-specific compartments, live cell imaging (LCI) is a method of choice. In this chapter, we compare currently used microscopy techniques and focus on considerations and requirements specific for LCI. Detailed protocols for LCI of Salmonella infection with either confocal laser scanning microscopy (CLSM) or spinning disk confocal microscopy (SDCM) are provided.
authors.affiliation: Abteilung Mikrobiologie, Fachbereich Biologie/Chemie, Universität Osnabrück, Barbarastr. 11, Osnabrück, 49076, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kehl
authors.forename: Michael
authors.initials: M
authors.lastname: Hensel
mesh_terms: D002470:Cell Survival; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods*; D019779:Salmonella enterica / Q000166:cytology* / Q000235:genetics / Q000502:physiology; D014162:Transfection