title: Methods for assessing the effects of galectins on leukocyte trafficking.
abstract: Numerous protocols exist for investigating leukocyte recruitment both in vitro and in vivo. Here we describe three of these methods; an in vitro flow chamber assay, intravital microscopy, and zymosan-induced peritonitis, and give details as to how they can be used to study the actions of galectins on this crucial process.
authors.affiliation: William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, Charterhouse Square, EC1M 6BQ, London, UK.
authors.forename: Beatrice R
authors.initials: BR
authors.lastname: Gittens
authors.forename: Rachael D
authors.initials: RD
authors.lastname: Wright
authors.forename: Dianne
authors.initials: D
authors.lastname: Cooper
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002465:Cell Movement / Q000187:drug effects; D002469:Cell Separation; D037161:Galectins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D055786:Gene Knockout Techniques; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007962:Leukocytes / Q000166:cytology* / Q000276:immunology; D008297:Male; D051379:Mice; D008853:Microscopy; D010538:Peritonitis / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism; D015054:Zymosan / Q000494:pharmacology
chemical_list: D037161:Galectins; D015054:Zymosan