title: [Determination of six chlorogenic acids in lonicerae flos extract by quantitative analysis multi-components with single marker].
abstract: This study is to determine six chlorogenic acids (chlorogenic acid, 5-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid and 4,5-di-O-caffeoylquinic acid) by quantitative analysis of multi-components with a single marker (QAMS). Chlorogeinc acid was used as internal reference to calculate the relative correction factors (RCF) of five compounds. Then the ruggedness of relative correction factors was tested on different instruments and columns. Meanwhile, a total of 4 batches of Lonicerae Flos and 20 batches of Lonicerae Flos extract with five different processing procedures were analyzed by external standard method (ESM) and QAMS, respectively. The ruggedness of relative correction factors was good. And the analytical results calculated by ESM and QAMS showed no difference. The quantitative method established was suitable for the quality evaluation of Lonicerae Flos extract.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Hui-Jun
authors.initials: HJ
authors.lastname: Li
authors.forename: Wen
authors.initials: W
authors.lastname: Gao
authors.forename: Ru
authors.initials: R
authors.lastname: Shen
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
authors.forename: Ping
authors.initials: P
authors.lastname: Li
mesh_terms: D015415:Biomarkers / Q000032:analysis; D002726:Chlorogenic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D035264:Flowers / Q000737:chemistry*; D031239:Lonicera / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D004365:Drugs, Chinese Herbal; D002726:Chlorogenic Acid