title: Synthesis and characterisation of multifunctional alginate microspheres via the in situ formation of ZnO quantum dots and the graft of 4-(1-pyrenyl) butyric acid to sodium alginate.
abstract: Growth factor-loaded fluorescent alginate microspheres, which can realise sustained growth factor release and fluorescence imaging, were synthesised by in situ formation of ZnO quantum dots (QDs) and covalent graft of 4-(1-pyrenyl) butyric acid (PBA). BSA was chosen as a growth factor model protein to study the release kinetic of growth factors from alginate microspheres. The microsphere size and fluorescent properties were also investigated. Investigations of cell culture were used for evaluating biocompatibility of BSA-loaded fluorescent microspheres and fluorescence imaging property of ZnO QDs and PBA-grafted sodium alginate from the microspheres. The results show that they have good fluorescent property either to microspheres or to cells and fluorescent microspheres have good biocompatibility and property in sustained release of growth factors. The obtained microspheres will be expected to realise the imaging of cells and materials and also the release of growth factor in tissue engineering or in cell culture.
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University , Chengdu , People's Republic of China.
authors.forename: Guilin
authors.initials: G
authors.lastname: Luo
authors.forename: Jianxin
authors.initials: J
authors.lastname: Wang
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Feng
authors.forename: Jie
authors.initials: J
authors.lastname: Weng
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D057809:HEK293 Cells; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008422:Materials Testing; D008863:Microspheres; D011721:Pyrenes / Q000737:chemistry / Q000494:pharmacology; D045663:Quantum Dots / Q000737:chemistry*; D015034:Zinc Oxide / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D011721:Pyrenes; C002218:1-pyrenebutyrate; D020723:Glucuronic Acid; D015034:Zinc Oxide
keywords: 4-(1-pyrenyl) butyric acid; ZnO QDs; cellar imaging; controlled release; fluorescent microspheres