title: Direct evidence for the function of crustacean insulin-like androgenic gland factor (IAG): total chemical synthesis of IAG.
abstract: Insulin-like androgenic gland factor (IAG) is presumed to be a sex differentiation factor so-called androgenic gland hormone (AGH) in decapod crustacean, although the function of IAG peptide has not yet been reported. In this study, we synthesized IAG from the prawn, Marsupenaeus japonicus, and its function was assessed by an in vitro bioassay. As a result, IAG with the insulin-type disulfide bond arrangement showed biological activity, whereas its disulfide isomer did not. These results strongly suggest that the native IAG peptide has an insulin-type disulfide, and it is the decapod AGH.
authors.affiliation: Department of Applied Biochemistry, School of Engineering, Tokai University, 4-1-1 Kitakaname, Hiratsuka, Kanagawa 259-1292, Japan. Electronic address: katay@tokai-u.jp.
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Katayama
authors.affiliation: Department of Applied Biochemistry, School of Engineering, Tokai University, 4-1-1 Kitakaname, Hiratsuka, Kanagawa 259-1292, Japan.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Kubota
authors.affiliation: Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Hironobu
authors.initials: H
authors.lastname: Hojo
authors.affiliation: Department of Biological Sciences, Faculty of Science, Kanagawa University, Hiratsuka, Kanagawa 259-1293, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Okada
authors.affiliation: Department of Biological Sciences, Faculty of Science, Kanagawa University, Hiratsuka, Kanagawa 259-1293, Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Kotaka
authors.affiliation: Ushimado Marine Institute, Faculty of Science, Okayama University, 130-17 Kashino, Ushimado, Setouchi, Okayama 701-4303, Japan.
authors.forename: Naoaki
authors.initials: N
authors.lastname: Tsutsui
authors.affiliation: Department of Biological Sciences, Faculty of Science, Kanagawa University, Hiratsuka, Kanagawa 259-1293, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Ohira
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology; D001181:Arthropods; D003400:Astacoidea / Q000187:drug effects / Q000378:metabolism; D002942:Circular Dichroism; D042341:Gonadal Hormones / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology; D008969:Molecular Sequence Data; D014819:Vitellogenins / Q000378:metabolism
chemical_list: D060829:Arthropod Proteins; D042341:Gonadal Hormones; D014819:Vitellogenins; C053644:androgenic gland hormone (Armadillidium)
keywords: Crustacean; Glycopeptide; Insulin-like androgenic gland factor; Marsupenaeus japonicus; Solid-phase peptide synthesis