title: Self-degradation of tissue adhesive based on oxidized dextran and poly-L-lysine.
abstract: We have developed a low-toxicity bioadhesive based on oxidized dextran and poly-L-lysine. Here, we report that the mechanical properties and degradation of this novel hydrogel bioadhesive can be controlled by changing the extent of oxidation of the dextran and the type or concentration of the anhydride species in the acylated poly-L-lysine. The dynamic moduli of the hydrogels can be controlled from 120 Pa to 20 kPa, suggesting that they would have mechanical compatibility with various tissues, and could have applications as tissue adhesives. Development of the hydrogel color from clear to brown indicates that the reaction between the dextran aldehyde groups and the poly-L-lysine amino groups may be induced by a Maillard reaction via Schiff base formation. Degradation of the aldehyde dextran may begin by reaction of the amino groups in the poly-L-lysine. The gel degradation can be ascribed to degradation of the aldehyde dextran in the hydrogel, although the aldehyde dextran itself is relatively stable in water. The oxidized dextran and poly-L-lysine, and the degraded hydrogel showed low cytotoxicities. These findings indicate that a hydrogel consisting of oxidized dextran and poly-L-lysine has low toxicity and a well-controlled degradation rate, and has potential clinical applications as a bioadhesive.
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Matsumura
authors.affiliation: BMG Incorporated, 45 Minamimatsunoki-cho, Higashikujo, Minami-ku, Kyoto 601-8023, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Nakajima
authors.affiliation: BMG Incorporated, 45 Minamimatsunoki-cho, Higashikujo, Minami-ku, Kyoto 601-8023, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Sugai
authors.affiliation: Center for Fiber and Textile Science, Kyoto Institute of Technology, Matsugasaki, Kyoto 606-8585, Japan. Electronic address: ma37ha73@kcn.jp.
authors.forename: Suong-Hyu
authors.initials: SH
authors.lastname: Hyon
mesh_terms: D000215:Acylation; D003116:Color; D003911:Dextrans / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D055595:Mechanical Phenomena; D010084:Oxidation-Reduction; D011107:Polylysine / Q000737:chemistry*; D014014:Tissue Adhesives / Q000737:chemistry*
chemical_list: D003911:Dextrans; D020100:Hydrogels; D014014:Tissue Adhesives; D011107:Polylysine
keywords: Bioadhesive; Biodegradation; Dextran; Hydrogel; Poly-l-lysine