title: The role of FGF-2/HGF and fibronectin matrix on pleomorphic adenoma myoepithelial cell morphology and immunophenotype: an in vitro study.
abstract: Myoepithelial cells play a central role in glandular tumors, regulating the progression of in situ to invasive neoplasias, with the tumor microenvironment being shown to be involved in both initiation and progression. This study aimed to analyze the in vitro effects of fibroblast growth factor-2 (FGF-2) and hepatocyte growth factor (HGF) in myoepithelial cells under the influence of the fibronectin matrix extracellular protein. Benign myoepithelial cells were obtained from pleomorphic adenoma and cultured on a fibronectin substratum. FGF-2 and HGF were supplemented at different concentrations and time intervals, in order to evaluate cell proliferation, morphology and immunophenotype. Individually, FGF-2 and HGF supplementation did not alter myoepithelial cell proliferation, morphology or immunophenotype. The fibronectin substratum provoked an increase in cell proliferation and immunopositivity for α-smooth muscle actin and FGF-2. The myoepithelial cell morphology changed when the fibronectin substratum and FGF-2 acted together, highlighting the importance of the fibronectin extracellular matrix protein on the behavior of these cells.
authors.affiliation: Department of Oral Pathology and.
authors.forename: Carolina Amália Barcellos
authors.initials: CA
authors.lastname: Silva
authors.forename: Laura Cristina Leite
authors.initials: LC
authors.lastname: Nardello
authors.forename: Frederico Windlin
authors.initials: FW
authors.lastname: Garcia
authors.forename: Ney Soares de
authors.initials: NS
authors.lastname: Araújo
authors.forename: Victor Angelo
authors.initials: VA
authors.lastname: Montalli
authors.forename: Vera Cavalcanti de
authors.initials: VC
authors.lastname: Araújo
authors.forename: Elizabeth Ferreira
authors.initials: EF
authors.lastname: Martinez
mesh_terms: D000199:Actins / Q000276:immunology; D008949:Adenoma, Pleomorphic / Q000276:immunology / Q000473:pathology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005353:Fibronectins / Q000494:pharmacology*; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D006801:Humans; D010641:Phenotype; D012468:Salivary Gland Neoplasms / Q000276:immunology / Q000473:pathology*
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D005353:Fibronectins; D016222:Fibroblast Growth Factor 2; D017228:Hepatocyte Growth Factor
keywords: Extracellular matrix; growth factors; myoepithelial cells; tumor microenvironment