title: Simultaneous determination of puerarin and its active metabolite in human plasma by UPLC-MS/MS: application to a pharmacokinetic study.
abstract: A rapid, selective and sensitive ultra-performance liquid chromatography (UPLC)-tandem mass spectrometry method about the simultaneous determination of puerarin and its major active metabolite, daidzein, in human plasma was developed and validated in order to investigate the pharmacokinetics (PKs) of Gegen after the usual oral dose administration to human. Chromatography was carried out on a Kinetex C18 column (2.1mm×50mm, 1.7μm) using 0.05% acetic acid in water and 0.05% acetic acid in methanol as mobile phase with a gradient elution. Liquid-liquid extraction with ethyl acetate in acidic condition could remove the interference and minimize the matrix effect of human plasma. The lower limit of quantification in human plasma was 0.2ng/mL for both of compounds, puerarin and daidzein. The calibration curves for puerarin and daidzein in human plasma were linear over all the concentration range of 0.2-100ng/mL with correlation coefficients greater than 0.998. This assay procedure was successfully applied to the PKs of puerarin and daidzein, after the usual oral dose of Gegen extract powder (2.56g, containing 9.984mg puerarin) in human subjects.
authors.affiliation: College of Pharmacy, CHA University, 59 Yatap-Ro, Bundang-gu, Seongnam-Si, Gyeonggi-Do 463-712, Republic of Korea.
authors.forename: Hyo-Rin
authors.initials: HR
authors.lastname: Jung
authors.affiliation: College of Pharmacy, CHA University, 59 Yatap-Ro, Bundang-gu, Seongnam-Si, Gyeonggi-Do 463-712, Republic of Korea.
authors.forename: Sook-Jin
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Jeonnam Development Institute for Korean Traditional Medicine, Jeollanam-do 288, Republic of Korea.
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Ham
authors.affiliation: Jeonnam Development Institute for Korean Traditional Medicine, Jeollanam-do 288, Republic of Korea.
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Cho
authors.affiliation: College of Pharmacy, Chonnam National University, 300 Yongbong-Dong, Buk-Gu, Gwangju 500-757, Republic of Korea.
authors.forename: Yong-Bok
authors.initials: YB
authors.lastname: Lee
authors.affiliation: College of Pharmacy, CHA University, 59 Yatap-Ro, Bundang-gu, Seongnam-Si, Gyeonggi-Do 463-712, Republic of Korea. Electronic address: hycho@cha.ac.kr.
authors.forename: Hea-Young
authors.initials: HY
authors.lastname: Cho
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D007529:Isoflavones / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D007723:Korea; D018517:Plant Roots / Q000737:chemistry; D029908:Pueraria / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D007529:Isoflavones; C004742:daidzein; C033607:puerarin
keywords: Daidzein; Human; Pharmacokinetics; Puerarin; UPLC-MS/MS