title: Mesenchymal stem cells are efficiently transduced with adenoviruses bearing type 35-derived fibers and the transduced cells with the IL-28A gene produces cytotoxicity to lung carcinoma cells co-cultured.
abstract: BACKGROUND
  Transduction of human mesenchymal stem cells (MSCs) with type 5 adenoviruses (Ad5) is limited in the efficacy because of the poor expression level of the coxsackie adenovirus receptor (CAR) molecules. We examined a possible improvement of Ad-mediated gene transfer in MSCs by substituting the fiber region of type 5 Ad with that of type 35 Ad.
  
  
  METHODS
  Expression levels of CAR and CD46 molecules, which are the major receptors for type 5 and type 35 Ad, respectively, were assayed with flow cytometry. We constructed vectors expressing the green fluorescent protein gene with Ad5 or modified Ad5 bearing the type 35 fiber region (AdF35), and examined the infectivity to MSCs with flow cytometry. We investigated anti-tumor effects of MSCs transduced with interleukin (IL)-28A gene on human lung carcinoma cells with a colorimetric assay. Expression of IL-28A receptors was tested with the polymerase chain reaction. A promoter activity of transcriptional regulatory regions in MSCs was determined with a luciferase assay and a tumor growth-promoting ability of MSCs was tested with co-injection of human tumor cells in nude mice.
  
  
  RESULTS
  MSCs expressed CD46 but scarcely CAR molecules, and subsequently were transduced with AdF35 but not with Ad5. Growth of MSCs transduced with the IL-28A gene remained the same as that of untransduced cells since MSCs were negative for the IL-28A receptors. The IL-28A-transduced MSCs however suppressed growth of lung carcinoma cells co-cultured, whereas MSCs transduced with AdF35 expressing the β-galactosidase gene did not. A regulatory region of the cyclooygenase-2 gene possessed transcriptional activities greater than other tumor promoters but less than the cytomegalovirus promoter, and MSCs themselves did not support tumor growth in vivo.
  
  
  CONCLUSIONS
  AdF35 is a suitable vector to transduce MSCs that are resistant to Ad5-mediated gene transfer. MSCs infected with AdF35 that activate an exogenous gene by the cytomegalovirus promoter can be a vehicle to deliver the gene product to targeted cells.
authors.forename: Takeo
authors.initials: T
authors.lastname: Suzuki
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Kawamura
authors.forename: Quanhai
authors.initials: Q
authors.lastname: Li
authors.forename: Shinya
authors.initials: S
authors.lastname: Okamoto
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tada
authors.forename: Koichiro
authors.initials: K
authors.lastname: Tatsumi
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shimada
authors.forename: Kenzo
authors.initials: K
authors.lastname: Hiroshima
authors.forename: Naoto
authors.initials: N
authors.lastname: Yamaguchi
authors.affiliation: Division of Pathology and Cell Therapy, Chiba Cancer Center Research Institute, Chiba, Japan. mtagawa@chiba-cc.jp.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Tagawa
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein / Q000235:genetics; D003602:Cytotoxicity, Immunologic; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D006801:Humans; D007378:Interleukins / Q000235:genetics*; D008175:Lung Neoplasms / Q000235:genetics* / Q000276:immunology*; D059630:Mesenchymal Stem Cells / Q000276:immunology* / Q000378:metabolism*; D051379:Mice; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014161:Transduction, Genetic; D000095022:Interferon Lambda
chemical_list: D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D007378:Interleukins; C000624353:interferon-lambda, human; D000095022:Interferon Lambda