title: Screening and characterization of oleaginous Chlorella strains and exploration of photoautotrophic Chlorella protothecoides for oil production.
abstract: The growth and oil production of nine Chlorella strains were comparatively assessed and Chlorellaprotothecoides CS-41 demonstrated the greatest lipid production potential. The effects of different nitrogen forms and concentrations, phosphorus concentrations and light intensities on growth and oil production were studied in laboratory columns. C. protothecoides CS-41 accumulated lipids up to 55% of dry weight, with triacylglycerol and oleic acid being 71% of total lipids and 59% of total fatty acids, respectively. High biomass and lipid productivities were achieved in outdoor panel PBRs, up to 1.25 and 0.59 g L(-1) day(-1), or 44. 1 and 16.1 g m(-2) day(-1), respectively. A two-stage cultivation strategy was proposed to enhance the algal biomass and lipid production. This is the first comprehensive investigation of both indoor and outdoor photoautotrophic C. protothecoides cultures for oil production, and C. protothecoides CS-41 represents a promising biofuel feedstock worthy of further exploration.
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Sun
authors.affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, PR China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Zhou
authors.affiliation: Department of Applied Sciences and Mathematics, Arizona State University Polytechnic Campus, Mesa, AZ 85212, USA.
authors.forename: Henri
authors.initials: H
authors.lastname: Gerken
authors.affiliation: Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, PR China; Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Baltimore, MD 21202, USA. Electronic address: jliu@umces.edu.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
mesh_terms: D052818:Autotrophic Processes / Q000187:drug effects / Q000528:radiation effects; D056804:Biofuels; D018533:Biomass; D002708:Chlorella / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D008027:Light; D008055:Lipids / Q000096:biosynthesis / Q000737:chemistry*; D009584:Nitrogen / Q000494:pharmacology; D009821:Oils / Q000378:metabolism*; D019301:Oleic Acid / Q000378:metabolism; D010758:Phosphorus / Q000494:pharmacology; D058433:Photobioreactors / Q000382:microbiology; D052817:Phototrophic Processes / Q000187:drug effects / Q000528:radiation effects; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism
chemical_list: D056804:Biofuels; D008055:Lipids; D009821:Oils; D014280:Triglycerides; D010758:Phosphorus; D019301:Oleic Acid; D009584:Nitrogen
keywords: Algae; Biofuels; Chlorella; Lipid; Outdoor photobioreactor