title: Stability-indicating HPLC method for the determination of cefquinome sulfate.
abstract: A novel and sensitive stability-indicating RP-HPLC method for the quantitative determination of cefquinome sulfate has been developed. Chromatographic separation and quantitative determination were performed using a high-performance liquid chromatograph with UV detection. As the stationary phase a LiChroCART RP-18 column (5 microm particle size, 125 mm x 4 mm, Merck, Darmstadt, Germany) was used. The mobile phase consisted of 10 volumes of acetonitrile and 90 volumes of an 0.02 M phosphate buffer (pH = 7.0). The flow rate of the mobile phase was 1.0 mL/min. The eluents were monitored by a UV-VIS detector at 268 nm. The drug was subjected to stress conditions of hydrolysis, oxidation, photolysis and thermal degradation. Significant degradation was found under basic, oxidizing stress and UV light. The developed method was validated with respect to linearity, accuracy, precision and robustness.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Dołhań
authors.forename: Anna
authors.initials: A
authors.lastname: Jelilńska
authors.forename: Monika
authors.initials: M
authors.lastname: Manuszewska
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D002511:Cephalosporins / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D004355:Drug Stability; D006868:Hydrolysis; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; C068212:cefquinome