title: Characterization of a novel antimicrobial peptide with chitin-biding domain from Mytilus coruscus.
abstract: Using reverse phase high performance liquid chromatography (RP-HPLC), a novel antimicrobial peptide with 55 amino acid residues was isolated from the hemolymph of Mytilus coruscus. This new antimicrobial peptide displays predominant antimicrobial activity against fungi and Gram-positive bacteria. The molecular mass and the N-terminal sequence of this peptide were analyzed by Mass Spectrometry and Edman degradation, respectively. This antimicrobial peptide, with molecular mass of 6621.55 Da, is characterized by a chitin-biding domain and by 6 Cysteine residues engaged in three intra-molecular disulfide bridges. The full-length of cDNA sequence of this new peptide was obtained by rapid amplification of cDNA ends (RACE) and the encoded precursor was turn out to be a chitotriosidase-like protein. Therefore, we named the precursor with mytichitin-1 and the new antimicrobial peptide (designated as mytichitin-CB) is the carboxyl-terminal part of mytichitin-1. The mRNA transcripts of mytichitin-1 are mainly detected in gonad and the expression level of mytichitin-1 in gonad was up-regulated and reached the highest level at 12 h after bacterial challenge, which was 9-fold increase compared to that of the control group. These results indicated that mytichitin-1 was involved in the host immune response against bacterial infection and might contribute to the clearance of invading bacteria.
authors.affiliation: Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, PR China.
authors.forename: Chuan-li
authors.initials: CL
authors.lastname: Qin
authors.affiliation: Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: Laboratory of Immunogenomics, Zhoushan Hospital, Zhoushan, 316022, PR China.
authors.forename: Shi-quan
authors.initials: SQ
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, PR China.
authors.forename: Xin-chao
authors.initials: XC
authors.lastname: Wang
authors.affiliation: Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, PR China.
authors.forename: Hui-hui
authors.initials: HH
authors.lastname: Liu
authors.affiliation: Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, PR China.
authors.forename: Mei-hua
authors.initials: MH
authors.lastname: Fan
authors.affiliation: Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, PR China.
authors.forename: Ri-xin
authors.initials: RX
authors.lastname: Wang
authors.affiliation: Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.affiliation: Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, PR China. Electronic address: liaozhi@zjou.edu.cn.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Liao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology; D001483:Base Sequence; D002686:Chitin / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005658:Fungi / Q000187:drug effects; D006066:Gonads / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006458:Hemolymph / Q000378:metabolism*; D016013:Likelihood Functions; D013058:Mass Spectrometry; D008957:Models, Genetic; D008969:Molecular Sequence Data; D049878:Mytilus / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D023181:Antimicrobial Cationic Peptides; D017931:DNA Primers; D018076:DNA, Complementary; D002686:Chitin
keywords: Antimicrobial peptide; Chitin-biding domain; Chitinase; Mytilus coruscus