title: Identification of linear B epitopes of pertactin of Bordetella pertussis induced by immunization with whole and acellular vaccine.
abstract: Pertussis is a serious infectious disease of the respiratory tract caused by the gram-negative bacteria Bordetella pertussis. There has been a reemergence of this disease within the population of several countries that have well established vaccination programs. Analyzes of clinical isolates suggest an antigenic divergence between the vaccine-based strains to the circulating strains. Although antibodies against P.69 are involved in the observed protective immunity, the sequences recognized as antigenic determinants in P.133, the precursor for P.69, P.3.4 and P.30, have not be determined. Here, the precise mapping of linear B-cell epitopes within the predicted P.133 pertactin sequences was accomplished using the SPOT-synthesis of peptide arrays onto cellulose membranes and screening with murine sera generated by vaccination with either the Pertussis cellular (miPc) or Pertussis acellular (miPa) vaccine. A total of 23 major epitopes were identified by sera from miPc vaccinated mice, while thirteen were identified by sera from miPa vaccinated mice. Of these epitopes, 12 epitopes were specifically identified by antibodies produced in response to the miPc vaccine and two were specific to the miPa vaccine. These epitopes were distributed throughout the pertactin sequence but a significant number were concentrated to the P.30 Prn segment. An analysis of the epitope correlation homologies indicated that the variations from the observed mutations in pertactin would not constitute a problem using these vaccines. In addition, the mapping of epitopes demonstrated a higher number of linear B-cell epitopes immunized with the Pc vaccine than the Pa vaccine.
authors.affiliation: Centro de Desenvolvimento Tecnológico em Saúde (CDTS)/Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas (INCT-IDN), Rio de Janeiro, RJ, Brazil; Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.
authors.forename: Flavio R
authors.initials: FR
authors.lastname: da Silva
authors.affiliation: Centro de Desenvolvimento Tecnológico em Saúde (CDTS)/Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas (INCT-IDN), Rio de Janeiro, RJ, Brazil.
authors.forename: Paloma
authors.initials: P
authors.lastname: Napoleão-Pego
authors.affiliation: Centro de Desenvolvimento Tecnológico em Saúde (CDTS)/Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas (INCT-IDN), Rio de Janeiro, RJ, Brazil; Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil; Departamento de Biologia Celular e Molecular, Universidade Federal Fluminense, Instituto de Biologia, Niterói, Rio de Janeiro, RJ, Brazil. Electronic address: dsimone@ioc.fiocruz.br.
authors.forename: Salvatore G
authors.initials: SG
authors.lastname: De-Simone
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001886:Bordetella pertussis / Q000276:immunology; D003429:Cross Reactions; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D010567:Pertussis Vaccine / Q000276:immunology*; D017434:Protein Structure, Tertiary; D022282:Vaccines, Acellular / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D018985:Epitopes, B-Lymphocyte; D010567:Pertussis Vaccine; D022282:Vaccines, Acellular; D010566:Virulence Factors, Bordetella; C068929:pertactin
keywords: B-epitope; Bordetela pertussis; Peptide array; Pertactin; SPOT-synthesis; Synthetic peptides