title: [Influence exogenous nicotinamide adenine dinucleotide (NAD+) on contractile and bioelectric activity of the rat heart].
abstract: This study is aimed to the investigation of the nicotinamide adenine dinucleotide (NAD+) effects and mechanisms of action in a heart. NAD+ (mcM) induces multiphase alternation of contractile activity of isolated rat heart: short positive inotropic action is followed by a negative inotropic phase. NAD+ (1-100 mcM) induces decreasing of action potential duration (APD) in rat atrial myocardium (from 45 +/- 0.82 ms in control experiments to 39 +/- 1.05 (n = 8) and 32 +/- 2 (n = 8) during application of 10 and 100 mcM of NAD+, respectively). Significant APD increase (from 45 +/- 0.82 ms to 74 +/- 1.89 (n = 8) ms) was observed during washing out of NAD+ (100 mcM). ATP or adenosine was unable to increase APD both during application or washing out. NAD+ induced APD decrease was not suppressed by P1-antagonist theophylline. P1-purinoreceptor and metabolite independent direct action of NAD+ in rat heart is suggested. Activation of P2X or P2Y receptors, cyclic ADP-ribose accumulation in cardiomyocytes is proposed as a main mechanism of NAD(+)-induced effects in the heart.
authors.forename: K B
authors.initials: KB
authors.lastname: Pustovit
authors.forename: V S
authors.initials: VS
authors.lastname: Kuz'min
authors.forename: G S
authors.initials: GS
authors.lastname: Sukhova
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D036563:Cyclic ADP-Ribose / Q000378:metabolism; D008297:Male; D009200:Myocardial Contraction / Q000502:physiology*; D009206:Myocardium / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D051381:Rats; D058469:Receptors, Purinergic P2X / Q000378:metabolism; D058470:Receptors, Purinergic P2Y / Q000378:metabolism
chemical_list: D058469:Receptors, Purinergic P2X; D058470:Receptors, Purinergic P2Y; D009243:NAD; D036563:Cyclic ADP-Ribose; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate