title: Carbapenem resistance in a human clinical isolate identified to be closely related to Acinetobacter indicus.
abstract: Here we report a case of carbapenem resistance in a human clinical isolate that was found to be closely related to the newly described environmental species Acinetobacter indicus. This strain harboured the blaOXA-23 carbapenemase gene located on a conjugative plasmid. Partial sequencing of 16S rDNA and rpoB genes, together with matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) analysis, showed that this strain was distantly related to the Acinetobacter baumannii-calcoaceticus complex and was closely related to A. indicus.
authors.affiliation: INSERM U914 'Emerging Resistance to Antibiotics', K.-Bicêtre, France.
authors.forename: Rémy A
authors.initials: RA
authors.lastname: Bonnin
authors.affiliation: INSERM U914 'Emerging Resistance to Antibiotics', K.-Bicêtre, France; Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland. Electronic address: laurent.poirel@unifr.ch.
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
authors.affiliation: Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Tanny J K
authors.initials: TJ
authors.lastname: van der Reijden
authors.affiliation: Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Lenie
authors.initials: L
authors.lastname: Dijkshoorn
authors.affiliation: Service de Bactériologie-Virologie, Hôpital Avicenne, Bobigny, France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Lescat
authors.affiliation: INSERM U914 'Emerging Resistance to Antibiotics', K.-Bicêtre, France; Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland.
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D000150:Acinetobacter / Q000737:chemistry / Q000145:classification* / Q000187:drug effects* / Q000302:isolation & purification; D000151:Acinetobacter Infections / Q000382:microbiology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015780:Carbapenems / Q000494:pharmacology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D010957:Plasmids; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; C466679:beta-lactamase OXA-23; D001618:beta-Lactamases
keywords: Acinetobacter; Carbapenemase; OXA-23; Taxonomy; β-Lactamase