title: Selective antibacterial activity of patchouli alcohol against Helicobacter pylori based on inhibition of urease.
abstract: The aim of this study is to evaluate the antibacterial activity and urease inhibitory effects of patchouli alcohol (PA), the bioactive ingredient isolated from Pogostemonis Herba, which has been widely used for the treatment of gastrointestinal disorders. The activities of PA against selected bacteria and fungi were determined by agar dilution method. It was demonstrated that PA exhibited selective antibacterial activity against Helicobacter pylori, without influencing the major normal gastrointestinal bacteria. Noticeably, the antibacterial activity of PA was superior to that of amoxicillin, with minimal inhibition concentration value of 78 µg/mL. On the other hand, PA inhibited ureases from H.pylori and jack bean in concentration-dependent fashion with IC50 values of 2.67 ± 0.79 mM and 2.99 ± 0.41 mM, respectively. Lineweaver-Burk plots indicated that the type of inhibition was non-competitive against H.pylori urease whereas uncompetitive against jack bean urease. Reactivation of PA-inactivated urease assay showed DL-dithiothreitol, the thiol reagent, synergistically inactivated urease with PA instead of enzymatic activity recovery. In conclusion, the selective H.pylori antibacterial activity along with urease inhibitory potential of PA could make it a possible drug candidate for the treatment of H.pylori infection.
authors.affiliation: College of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510006, PR China.
authors.forename: Xiao-Dan
authors.initials: XD
authors.lastname: Yu
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Xie
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Wang
authors.forename: Yu-Cui
authors.initials: YC
authors.lastname: Li
authors.forename: Zhi-Zhun
authors.initials: ZZ
authors.lastname: Mo
authors.forename: Yi-Feng
authors.initials: YF
authors.lastname: Zheng
authors.forename: Ji-Yan
authors.initials: JY
authors.lastname: Su
authors.forename: Ye-er
authors.initials: YE
authors.lastname: Liang
authors.forename: Jin-Zhi
authors.initials: JZ
authors.lastname: Liang
authors.forename: Zi-Ren
authors.initials: ZR
authors.lastname: Su
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
mesh_terms: D000658:Amoxicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000201:enzymology; D019686:Lamiaceae / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D012717:Sesquiterpenes / Q000494:pharmacology*; D014510:Urease / Q000037:antagonists & inhibitors*
chemical_list: D000900:Anti-Bacterial Agents; D012717:Sesquiterpenes; D000658:Amoxicillin; D014510:Urease; C052983:patchouli alcohol
keywords: Helicobacter pylori; antibacterial; non-competitive inhibitor; patchouli alcohol; urease