title: DNA-hosted Hoechst dyes: application for label-free fluorescent monitoring of endonuclease activity and inhibition.
abstract: A simple and facile approach was developed for monitoring EcoRI endonuclease activity and inhibition, in which a hairpin-like DNA containing restriction cutting site for EcoRI endonuclease acts as the sensing element and Hoechst dyes as the signal indicator in a label-free format.
authors.affiliation: Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China. bcye@ecust.edu.cn.
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Jiang
authors.forename: Su-Miao
authors.initials: SM
authors.lastname: Guo
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.forename: Bang-Ce
authors.initials: BC
authors.lastname: Ye
mesh_terms: D004247:DNA / Q000737:chemistry*; D004720:Endonucleases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D004247:DNA; D004720:Endonucleases