title: Protective effect of a prime-boost strategy with the Ts87 vaccine against Trichinella spiralis infection in mice.
abstract: Trichinellosis is a widespread zoonosis primarily caused by Trichinella spiralis. Mucosal immunity is crucial for preventing Trichinella spiralis infection. In our previous study, a DNA vaccine with the Trichinella antigen Ts87 delivered by an attenuated Salmonella typhimurium elicited partial protection against Trichinella spiralis infection in mice. In the current study, to elicit a more robust immune response and develop a potent vaccination strategy against trichinellosis, a heterologous prime-boost vaccination regimen for Ts87 was used in mice and the protective efficacy was evaluated compared to the homologous DNA prime-boost or protein prime-boost immunization alone. The results revealed that the DNA-prime/protein-boost vaccination with Ts87 induced higher levels of both humoral and cellular immune responses. The challenge results showed that mice with the DNA-prime/protein-boost vaccination displayed higher muscle larval reduction than those immunized with DNA prime-boost or protein prime-boost. The results demonstrated that mice vaccinated with Ts87 in a DNA-prime/protein-boost strategy effectively elicited a local IgA response and mixed Th1/Th2 immune response that might be responsible for improved protection against Trichinella spiralis infection.
authors.affiliation: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Department of Pediatrics, Section of Tropical Medicine, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Bin
authors.identifier: 0000-0002-6884-9452
authors.initials: B
authors.lastname: Zhan
authors.affiliation: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.
authors.forename: Xiaodi
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.
authors.forename: Kuo
authors.initials: K
authors.lastname: Bi
authors.affiliation: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.
authors.forename: Xinping
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*; D005260:Female; D007117:Immunization, Secondary / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D016896:Treatment Outcome; D017160:Trichinella spiralis / Q000187:drug effects*; D014235:Trichinellosis / Q000276:immunology* / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use*
chemical_list: D000947:Antigens, Helminth; D019444:Vaccines, DNA