title: Carboxymethylation of an exopolysaccharide from Lachnum and effect of its derivatives on experimental chronic renal failure.
abstract: Carboxymethylated polysaccharide CLEP-1b was prepared from a single component (LEP-1b) of Lachnum YM281 exopolysaccharides by molecular modification with a degree of substitution (DS) of 0.286. Infrared result proved that the carboxymethylation of LEP-1b succeeded and (13)C NMR result showed that the carboxymethyl group (CH2COOH) was chemically linked to an oxygen (O) atom of the hydroxyl on C-3 of LEP-1b. LEP-1b could improve the histopathological status of kidney and significantly reduce the contents of serum creatinine (Scr) and blood urea nitrogen (BUN), and increase the contents of total protein and albumin. It could also enhance the activity of SOD, GSH-PX, CAT, GSH and decrease MDA contents in the nephridial and hepatic tissues. What's more, CLEP-1b showed more significant effects than LEP-1b at the same dosage. The research indicated that LEP-1b and CLEP-1b could mitigate the chronic renal failure of mice and the effects were closely associated with antioxidant activity.
authors.affiliation: Microbial Resources and Application Laboratory, College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Yanna
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Microbial Resources and Application Laboratory, College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China. Electronic address: yeming123@sina.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Ye
authors.affiliation: Microbial Resources and Application Laboratory, College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Zhanzhan
authors.initials: Z
authors.lastname: Du
authors.affiliation: Microbial Resources and Application Laboratory, College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Lianyan
authors.initials: L
authors.lastname: Jing
authors.affiliation: Microbial Resources and Application Laboratory, College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Maheen Mahwish
authors.initials: MM
authors.lastname: Surhio
authors.affiliation: Microbial Resources and Application Laboratory, College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000627:therapeutic use; D001203:Ascomycota / Q000737:chemistry*; D007668:Kidney / Q000187:drug effects; D007676:Kidney Failure, Chronic / Q000188:drug therapy*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D011134:Polysaccharides / Q000737:chemistry* / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D011134:Polysaccharides
keywords: Antioxidant activity; Carboxymethylation; Chronic renal failure; Lachnum polysaccharides