title: Evaluation of antioxidant activities of ampelopsin and its protective effect in lipopolysaccharide-induced oxidative stress piglets.
abstract: The aim of this study was to investigate the antioxidant potential of ampelopsin (APS) by using various methods in vitro, as well as to determine effects of APS on LPS-induced oxidative stress in piglets. The results showed that APS exhibited excellent free radical scavenging by DPPH, ABTS, O2•-, H2O2 and ferric reducing antioxidant power. Ampelopsin also protected pig erythrocytes against AAPH-induced apoptosis and hemolysis, decreased total superoxide dismutase activity, and increased lipid peroxidation. Furthermore the results demonstrated that APS enhanced the total antioxidant capacity and decreased the malondialdehyde and protein carbonyl contents in LPS-treated piglets. The results of the present investigation suggest that APS possesses a strong antioxidant activity and alleviates LPS-induced oxidative stress, possibly due to its ability to prevent reactive oxygen species.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, P.R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Hou
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, P.R. China.
authors.forename: Jingfei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, P.R. China.
authors.forename: Hussain
authors.initials: H
authors.lastname: Ahmad
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, P.R. China; Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, P.R. China.
authors.forename: Ziwei
authors.initials: Z
authors.lastname: Xu
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, P.R. China.
authors.forename: Tian
authors.initials: T
authors.lastname: Wang
mesh_terms: D000578:Amidines / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D052160:Benzothiazoles / Q000037:antagonists & inhibitors; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation / Q000187:drug effects; D008070:Lipopolysaccharides; D008315:Malondialdehyde / Q000037:antagonists & inhibitors / Q000378:metabolism; D016877:Oxidants / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D010851:Picrates / Q000037:antagonists & inhibitors; D050050:Protein Carbonylation / Q000187:drug effects; D013451:Sulfonic Acids / Q000037:antagonists & inhibitors; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000037:antagonists & inhibitors; D013552:Swine
chemical_list: D000578:Amidines; D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005419:Flavonoids; D008070:Lipopolysaccharides; D016877:Oxidants; D010851:Picrates; D013451:Sulfonic Acids; D013481:Superoxides; C106407:ampelopsin; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D008315:Malondialdehyde; C046728:2,2'-azobis(2-amidinopropane); D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D013482:Superoxide Dismutase