title: Novel pH-responsive dextrin nanogels for doxorubicin delivery to cancer cells with reduced cytotoxicity to cardiomyocytes and stem cells.
abstract: The aim of this study was to develop pH-responsive dextrin nanogels (DNGs) capable of triggered intracellular DOX release at the lower pH of cancer cells. DNGs were prepared by an emulsion cross-linking method using glyoxal as cross-linker to create an acid-labile bond. A higher molecular weight of dextrin with increasing mole ratio of dextrin to glyoxal decreased the average diameter of DNGs. DNGs showed slightly negative surface charge and pH-responsive behavior. The in vitro drug release was slow at pH 7.4 and increased with decreasing pH (pH 5>6.8). The cytotoxicity of DOX-loaded DNGs in mesenchymal stem cells and cardiomyocytes was lower than that of free DOX. Moreover, DOX-loaded DNGs were efficiently internalized by tumor cells with rapid release of DOX into the nucleus. Thus, DOX-loaded DNGs were successful for intracellular targeted anti-tumor drug delivery and reducing side-effects to non-tumor cells such as cardiomyocytes and stem cells.
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand; Pharmaceutical Biopolymer Group (PBiG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand.
authors.forename: Somkamol
authors.initials: S
authors.lastname: Manchun
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand; Pharmaceutical Biopolymer Group (PBiG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand.
authors.forename: Kamonrak
authors.initials: K
authors.lastname: Cheewatanakornkool
authors.affiliation: School of Pharmacy, Faculty of Health Sciences, Curtin University, Bentley 6102, Australia; Biosciences Research Precinct, Curtin University, Bentley 6102, Australia.
authors.forename: Crispin R
authors.initials: CR
authors.lastname: Dass
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand; Pharmaceutical Biopolymer Group (PBiG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. Electronic address: pornsak@su.ac.th.
authors.forename: Pornsak
authors.initials: P
authors.lastname: Sriamornsak
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D003912:Dextrins / Q000008:administration & dosage / Q000378:metabolism*; D004317:Doxorubicin / Q000008:administration & dosage / Q000378:metabolism*; D016503:Drug Delivery Systems / Q000379:methods; D006863:Hydrogen-Ion Concentration; D051379:Mice; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism*; D000080385:Nanogels; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000378:metabolism*; D011094:Polyethyleneimine / Q000008:administration & dosage / Q000378:metabolism*; D051381:Rats; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism*
chemical_list: D003912:Dextrins; D000080385:Nanogels; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; D004317:Doxorubicin; D011094:Polyethyleneimine
keywords: Cancer; Dextrin; Doxorubicin; Nanogels; pH-responsive