title: p28, a truncated form of TRα1 regulates mitochondrial physiology.
abstract: We have previously identified in mitochondria two truncated forms of the T3 nuclear receptor TRα1, with molecular weights of 43kDa (p43) and 28kDa (p28) respectively located in the matrix and in the inner membrane. Previously, we have demonstrated that p43 stimulates mitochondrial transcription and protein synthesis in the presence of T3. Here we report that p28 is targeted into the organelle in a T3-dependent manner and displays an affinity for T3 higher than the nuclear receptor. We tried to generate mice overexpressing p28 using the human α-skeletal actin promoter, however we found an early embryonic lethality that was probably linked to a transient expression of p28 in trophoblast giant cells. This could be partly explained by the observation that overexpression of p28 in human fibroblasts induced alterations of mitochondrial physiology.
authors.affiliation: INRA, UMR866 Dynamique Musculaire et Métabolisme, 2 place Viala, F-34060 Montpellier, France; Université Montpellier I et II, F-34060 Montpellier, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Pessemesse
authors.affiliation: INRA, UMR866 Dynamique Musculaire et Métabolisme, 2 place Viala, F-34060 Montpellier, France; Université Montpellier I et II, F-34060 Montpellier, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Lepourry
authors.affiliation: INRA, UMR866 Dynamique Musculaire et Métabolisme, 2 place Viala, F-34060 Montpellier, France; Université Montpellier I et II, F-34060 Montpellier, France.
authors.forename: Katia
authors.initials: K
authors.lastname: Bouton
authors.affiliation: INRA, UMR866 Dynamique Musculaire et Métabolisme, 2 place Viala, F-34060 Montpellier, France; Université Montpellier I et II, F-34060 Montpellier, France.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Levin
authors.affiliation: INRA, UMR866 Dynamique Musculaire et Métabolisme, 2 place Viala, F-34060 Montpellier, France; Université Montpellier I et II, F-34060 Montpellier, France.
authors.forename: Gérard
authors.initials: G
authors.lastname: Cabello
authors.affiliation: INRA, UMR866 Dynamique Musculaire et Métabolisme, 2 place Viala, F-34060 Montpellier, France; Université Montpellier I et II, F-34060 Montpellier, France.
authors.forename: Chantal
authors.initials: C
authors.lastname: Wrutniak-Cabello
authors.affiliation: INRA, UMR866 Dynamique Musculaire et Métabolisme, 2 place Viala, F-34060 Montpellier, France; Université Montpellier I et II, F-34060 Montpellier, France. Electronic address: casasf@supagro.inra.fr.
authors.forename: François
authors.initials: F
authors.lastname: Casas
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism; D010929:Placentation; D011247:Pregnancy; D021381:Protein Transport; D051381:Rats; D011988:Receptors, Thyroid Hormone / Q000235:genetics* / Q000378:metabolism; D017384:Sequence Deletion; D014284:Triiodothyronine / Q000378:metabolism
chemical_list: D010446:Peptide Fragments; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine
keywords: Mitochondria; Triiodothyronine