title: [Regulation of the binding of the BMP-2 growth factor with collagen by blood plasma fibronectin].
abstract: The release kinetics of recombinant human bone morphogenic factor 2 (rhBMP-2) from collageneous hydrogel in the presence of human blood plasma have been studied. The expulsion of rhBMP-2 from the collagen-BMP-2 complex by the competitive adhesion of collagen-binding proteins penetrating from plasma was firstly recognized. It was experimentally proven that that blood plasma fibronectin is the main collagen-binding protein, which is responsible for the controlled release of rhBMP-2. As a result, a new collageneous hydrogel with the incorporation of fibronectin was created which retained rhBMP-2 for a twice longer period as compared to the ordinary collageneous hydrogel. A distinctive feature of this new collagen-fibronectin matrix is the slow release of rhBMP-2 in the first three days which allows for the avoiding of adverse effects in clinics caused by the rapid release of large amounts of rhBMP-2 from collageneous hydrogel.
authors.forename: E O
authors.initials: EO
authors.lastname: Osidak
authors.forename: M S
authors.initials: MS
authors.lastname: Osidak
authors.forename: D E
authors.initials: DE
authors.lastname: Sivogrivov
authors.forename: T S
authors.initials: TS
authors.lastname: Portnaia
authors.forename: T M
authors.initials: TM
authors.lastname: Grunina
authors.forename: L A
authors.initials: LA
authors.lastname: Soboleva
authors.forename: V G
authors.initials: VG
authors.lastname: Lunin
authors.forename: A S
authors.initials: AS
authors.lastname: Kariagina
authors.forename: S P
authors.initials: SP
authors.lastname: Domogatskiĭ
mesh_terms: D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D003692:Delayed-Action Preparations; D005353:Fibronectins / Q000737:chemistry*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D007700:Kinetics; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D003692:Delayed-Action Preparations; D005353:Fibronectins; D020100:Hydrogels; D011994:Recombinant Proteins; D003094:Collagen