title: Chryseobacterium takakiae sp. nov., a member of the phylum Bacteroidetes isolated from Takakia lepidozioides.
abstract: A Gram-stain-negative, rod-shaped and non-endospore-forming bacterium, designated strain AG1-2(T), was isolated from Takakia lepidozioides collected from the Gawalong glacier in Tibet, China and characterized using a polyphasic taxonomic approach. The predominant fatty acids of strain AG1-2(T) were iso-C15 : 0 (36.0 %), iso-C17:0 3-OH (20.2 %), summed feature 9 (iso-C17:1ω9c and/or C16:0 10-methyl, 16.4%) and summed feature 3 (C16:1ω7c and/or C16:1ω6c, 11.1%). The major polar lipids were phosphatidylethanolamine, three unidentified aminolipids and two unidentified lipids. Strain AG1-2(T) contained MK-6 as the dominant menaquinone, and the genomic DNA G+C content was 37.3 mol%. The phylogenetic analysis based on the 16S rRNA gene sequences showed that strain AG1-2(T) was affiliated to species of the genus Chryseobacterium, and its closest related species were Chryseobacterium taiwanense Soil-3-27(T), Chryseobacterium hispalense AG13(T), Chryseobacterium camelliae THG C4-1(T) and Chryseobacterium taeanense PHA3-4(T) with a sequence similarity of 98.0, 97.8, 97.3 and 97.1%, respectively. However, the DNA-DNA relatedness values between these strains and strain AG1-2(T) were 29, 21, 21 and 45%, respectively. Based on phylogenetic inference and phenotypic data, strain AG1-2(T) is considered to represent a novel species of the genus Chryseobacterium, for which the name Chryseobacterium takakiae sp. nov. is proposed. The type strain is AG1-2(T) ( = CGMCC 1.12488(T) = DSM 26898(T)).
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhao
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Xin Yao
authors.initials: XY
authors.lastname: Chen
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Xue Dong
authors.initials: XD
authors.lastname: Li
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Zhi Ling
authors.initials: ZL
authors.lastname: Chen
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Yan Hong
authors.initials: YH
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D044002:Bryophyta / Q000382:microbiology*; D045247:Chryseobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D046448:Ice Cover; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D018609:Tibet; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6