title: [Research about formulas for activating blood and resolving stasis Xuesaitong capsule regulate CD117+ hemopoietic stem cell to produce new blood].
abstract: OBJECTIVE
  To investigate the mechanism that the formulas for activating blood and resolving stasis can regulate hemopoietic stem cell to produce new blood.
  
  
  METHODS
  Rats were established animal model of acute cerebral infarction by referencing Olivette' method. They were randomly divided into model group, the group of the high, middle, low dose of the formulas for activating blood and resolving stasis. Each group and then wasrandomly divided into subgroups by 1, 3, 7, 14, 28 d. Xuesaitong capsule was formulated into 20, 40, 60 g x L(-1) with normal saline. The rats were given gavage drugs once a day until the experient ended, and the model group was administrated by intragastrical perfusion of normal saline. ELISA was used to detect the expression of SCF in peripheral blood and bone marrow among different groups at different time points. Flow cytometry was used to observe the changes of CD117 in blood and bone marrow.
  
  
  RESULTS
  The CD117+ HSC and SCF concentration in peripheral blood and bone marrow of model group were increasing during 1-14 d,there was a peak on the 14th day, then the expression was reducing. CD117+ HSC and SCF concentration rising trend in the group of the high, middle dose of the formulas for activating blood and resolving stasis was preceded model group (P < 0.05).
  
  
  CONCLUSIONS
  Activating blood and resolving stasis can regulate hemopoietic stem cell to produce new blood, and it is through the regulation of CD117+ HSC number to achieve the purpose.
authors.forename: Bao-Xia
authors.initials: BX
authors.lastname: Zhang
authors.forename: Jin-Sheng
authors.initials: JS
authors.lastname: Zhang
authors.forename: Mei-Mei
authors.initials: MM
authors.lastname: Du
authors.forename: Yang-Yang
authors.initials: YY
authors.lastname: Zhang
authors.forename: Hui-Fang
authors.initials: HF
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D002214:Capsules; D002544:Cerebral Infarction / Q000097:blood / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D002626:Chemistry, Pharmaceutical; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008297:Male; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D019089:Stem Cell Factor / Q000235:genetics / Q000378:metabolism
chemical_list: D002214:Capsules; D004365:Drugs, Chinese Herbal; D019089:Stem Cell Factor; D019009:Proto-Oncogene Proteins c-kit