title: Correlations between polymorphisms of extracellular superoxide dismutase, aldehyde dehydrogenase-2 genes, as well as drinking behavior and pancreatic cancer.
abstract: OBJECTIVE
  To investigate the correlation between drinking behavior combined with polymorphisms of extracellular superoxide dismutase (EC-SOD) and aldehyde dehydrogenase-2 (ALDH2) genes and pancreatic cancer.
  
  
  METHODS
  The genetic polymorphisms of EC-SOD and ALDH2 were analyzed by polymerase chain reaction restriction fragment length polymorphism in the peripheral blood leukocytes obtained from 680 pancreatic cancer cases and 680 non-cancer controls. Subsequently the frequency of genotype was compared between the pancreatic cancer patients and the healthy controls.The relationship of drinking with pancreatic cancer was analyzed.
  
  
  RESULTS
  The frequencies of EC-SOD (C/G) and ALDH2 variant genotypes were 37.35% and 68.82% respectively in the pancreatic cancer cases, and were significantly higher than those in the healthy controls (21.03% and 44.56%, all P<0.01). People who carried EC-SOD (C/G) (OR=2.24, 95% CI= 1.81-4.03, P<0.01) or ALDH2 variant genotypes (OR=2.75, 95% CI=1.92-4.47, P<0.01) had a high risk to develop pancreatic cancer. Those who carried EC-SOD (C/G) genotype combined with ALDH2 variant genotype had a high risk for pancreatic cancer (29.56% vs. 6.76%, OR=7.69, 95% CI=3.58-10.51, P<0.01). The drinking rate of the pancreatic cancer group (64.12%) was significantly higher than that of the control group (40.15%; OR=2.66, 95% CI=1.30-4.42, P<0.01). An interaction between drinking and EC-SOD (C/G)/ALDH2 variant genotypes increased the risk of occurrence of pancreatic cancer (OR=25.00, 95% CI= 11.87-35.64, P<0.01).
  
  
  CONCLUSIONS
  EC-SOD (C/G), ALDH2 variant genotypes and drinking might be the risk factors of pancreatic cancer.
authors.affiliation: Department of Stomatology, First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, China.
authors.forename: Chao-xian
authors.initials: CX
authors.lastname: Zhang
authors.affiliation: Department of Stomatology, First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, China.
authors.forename: Yong-mei
authors.initials: YM
authors.lastname: Qin
authors.affiliation: 2Department of Stomatology, First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, China.
authors.forename: Li-ke
authors.initials: LK
authors.lastname: Guo
mesh_terms: D000428:Alcohol Drinking; D000444:Aldehyde Dehydrogenase / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors; D013482:Superoxide Dismutase / Q000235:genetics*
chemical_list: D017931:DNA Primers; D013482:Superoxide Dismutase; D000444:Aldehyde Dehydrogenase