title: α-Lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction associated to inflammatory conditions.
abstract: An adequate redox status is important for maintaining mitochondrial function in inflammatory conditions. The aim of this work was to evaluate the effects of α-lipoic acid (LA) in kidney oxidative metabolism and mitochondrial function in lipopolysaccharide (LPS) treated rats. Sprague-Dawley rats (female, 45 ± 5 days old) were treated with LPS (10 mg kg(-1)) and/or LA (100 mg kg(-1)). It was observed in LPS-treated animals that the LA prevented the increase in 1.2 fold of NO production, decreased (30-40%) mitochondrial complex I-III and IV activities, and decreased (26%) membrane potential and cardiolipin oxidation (76%). No differences were observed in mitochondrial O2 consumption, mitochondrial complex II-III activity, and ATP production when LPS group was compared to LA + LPS group. Based on the improvement of mitochondrial function, the decreased production of mitochondrial NO and restoration of cardiolipin levels, this work provides a new evidence that α-lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction.
authors.affiliation: Institute of Biochemistry and Molecular Medicine, School of Pharmacy and Biochemistry, University of Buenos Aires-CONICET, Junín 956, C1113AAD Buenos Aires, Argentina. salvarez@ffyb.uba.ar.
authors.forename: Maria Cecilia
authors.initials: MC
authors.lastname: Cimolai
authors.forename: Virginia
authors.initials: V
authors.lastname: Vanasco
authors.forename: Timoteo
authors.initials: T
authors.lastname: Marchini
authors.forename: Natalia D
authors.initials: ND
authors.lastname: Magnani
authors.forename: Pablo
authors.initials: P
authors.lastname: Evelson
authors.forename: Silvia
authors.initials: S
authors.lastname: Alvarez
mesh_terms: D000818:Animals; D005260:Female; D007249:Inflammation / Q000188:drug therapy* / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D020011:Protective Agents / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D008063:Thioctic Acid / Q000008:administration & dosage*
chemical_list: D020011:Protective Agents; D008063:Thioctic Acid