title: The nano-bio interface mapped by oxidative footprinting of the adsorption sites of myoglobin.
abstract: Oxidative footprinting has been used to study the structure of macromolecular assemblies such as protein-protein and protein-ligand complexes. We propose a novel development of this technique to probe the protein corona that forms at the surface of nanoparticles in any biological medium. Indeed, very few techniques allow studying this interface at the molecular and residue level. Based on hydroxyl radical-mediated oxidation of proteins and analysis by nanoscale liquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS), two sites of adsorption of myoglobin on silica nanoparticles are identified. This method gives new insights in the understanding of protein adsorption on nanomaterials.
authors.affiliation: CEA Saclay, LIDyL, Physico-Chimie sous Rayonnement, UMR 3299 CEA-CNRS, Bat 546, 91191, Gif-sur-Yvette Cedex, France, stephanie.devineau@ens.fr.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Devineau
authors.forename: Christelle
authors.initials: C
authors.lastname: Mathé
authors.forename: Véronique
authors.initials: V
authors.lastname: Legros
authors.forename: Florence
authors.initials: F
authors.lastname: Gonnet
authors.forename: Régis
authors.initials: R
authors.lastname: Daniel
authors.forename: Jean Philippe
authors.initials: JP
authors.lastname: Renault
authors.forename: Serge
authors.initials: S
authors.lastname: Pin
mesh_terms: D000327:Adsorption; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D006736:Horses; D008958:Models, Molecular; D009211:Myoglobin / Q000737:chemistry* / Q000302:isolation & purification; D053758:Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D012822:Silicon Dioxide / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D009211:Myoglobin; D012822:Silicon Dioxide