title: Nanostructured materials from hydroxyethyl cellulose for skin tissue engineering.
abstract: In this study, a novel fibrous membrane of hydroxyethyl cellulose (HEC)/poly(vinyl alcohol) blend was successfully fabricated by electrospinning technique and characterized. The concentration of HEC (5%) with PVA (15%) was optimized, blended in different ratios (30-50%) and electrospun to get smooth nanofibers. Nanofibrous membranes were made water insoluble by chemically cross-linking by glutaraldehyde and used as scaffolds for the skin tissue engineering. The microstructure, morphology, mechanical and thermal properties of the blended HEC/PVA nanofibrous scaffolds were characterized by scanning electron microscope, Fourier transform infrared spectroscopy, differential scanning colorimetry, universal testing machine and thermogravimetric analysis. Cytotoxicity studies on these nanofibrous scaffolds were carried out using human melanoma cells by the MTT assays. The cells were able to attach and spread in the nanofibrous scaffolds as shown by the SEM images. These preliminary results show that these nanofibrous scaffolds that supports cell adhesion and proliferation is promising for skin tissue engineering.
authors.affiliation: Faculty of Industrial Sciences & Technology, University Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia. Electronic address: fhanani01@yahoo.com.
authors.forename: Farah Hanani
authors.initials: FH
authors.lastname: Zulkifli
authors.affiliation: Faculty of Industrial Sciences & Technology, University Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia.
authors.forename: Fathima Shahitha Jahir
authors.initials: FSJ
authors.lastname: Hussain
authors.affiliation: Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, Bandar Indera Mahkota Campus, Jalan Sultan Ahmad Shah, 25200 Kuantan, Pahang, Malaysia.
authors.forename: Mohammad Syaiful Bahari Abdull
authors.initials: MSBA
authors.lastname: Rasad
authors.affiliation: Faculty of Industrial Sciences & Technology, University Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia.
authors.forename: Mashitah
authors.initials: M
authors.lastname: Mohd Yusoff
mesh_terms: D002152:Calorimetry, Differential Scanning; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry* / Q000627:therapeutic use; D012867:Skin / Q000166:cytology*; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D002482:Cellulose; C002283:hydroxyethylcellulose
keywords: Electrospinning; Hydroxyethyl cellulose; Nanofibrous scaffolds; Skin tissue engineering