title: Purification of a low molecular weight fucoidan for SPECT molecular imaging of myocardial infarction.
abstract: Fucoidans constitute a large family of sulfated polysaccharides with several biochemical properties. A commercial fucoidan from brown algae, containing low molecular weight polysaccharidic species constituted of l-fucose, uronic acids and sulfate groups, was simply treated here with calcium acetate solution. This treatment led to a purified fraction with a yield of 45%. The physicochemical characterizations of the purified fucoidan using colorimetric assay, MALLS, dRI, FT-IR, NMR, exhibited molecular weight distributions and chemical profiles similar for both fucoidans whereas the sulfate and l-fucose contents increased by 16% and 71%, respectively. The biodistribution study in rat of both compounds labeled with 99mTc evidenced a predominant renal elimination of the purified fucoidan, but the crude fucoidan was mainly retained in liver and spleen. In rat myocardial ischemia-reperfusion, we then demonstrated the better efficiency of the purified fucoidan. This purified sulfated polysaccharide appears promising for the development of molecular imaging in acute coronary syndrome.
authors.affiliation: Inserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, France. pierre.saboural@univ-paris13.fr.
authors.forename: Pierre
authors.initials: P
authors.lastname: Saboural
authors.affiliation: Inserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, France. frederic.chaubet@univ-paris13.fr.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Chaubet
authors.affiliation: Inserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, France. francois.rouzet@bch.aphp.fr.
authors.forename: Francois
authors.initials: F
authors.lastname: Rouzet
authors.affiliation: Inserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, France.
authors.forename: Faisal
authors.initials: F
authors.lastname: Al-Shoukr
authors.affiliation: Inserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, France. rana.ben-azzouna@bch.aphp.fr.
authors.forename: Rana Ben
authors.initials: RB
authors.lastname: Azzouna
authors.affiliation: Laboratory CSPBAT, Paris 13 University, Sorbonne Paris Cité, CNRS UMR 7244, SBMB team, F-93017, Bobigny, France. nbouchemal@smbh.univ-paris13.fr.
authors.forename: Nadia
authors.initials: N
authors.lastname: Bouchemal
authors.affiliation: Laboratory of Polymers Biopolymers Surfaces, Normandie University, Rouen University, F-76821, Mont Saint Aignan, France. luc.picton@univ-rouen.fr.
authors.forename: Luc
authors.initials: L
authors.lastname: Picton
authors.affiliation: Inserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, France. liliane.louedec@inserm.fr.
authors.forename: Liliane
authors.initials: L
authors.lastname: Louedec
authors.affiliation: Inserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, France. murielle.maire@univ-paris13.fr.
authors.forename: Murielle
authors.initials: M
authors.lastname: Maire
authors.affiliation: Algues & Mer, Kernigou, F-29242, Ouessant, France. lydia.rolland@algues-et-mer.com.
authors.forename: Lydia
authors.initials: L
authors.lastname: Rolland
authors.affiliation: Algues & Mer, Kernigou, F-29242, Ouessant, France. guy.potier@algues-et-mer.com.
authors.forename: Guy
authors.initials: G
authors.lastname: Potier
authors.affiliation: Inserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, France. dominique.leguludec@bch.aphp.fr.
authors.forename: Dominique Le
authors.initials: DL
authors.lastname: Guludec
authors.affiliation: Inserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, France. didier.letourneur@inserm.fr.
authors.forename: Didier
authors.initials: D
authors.lastname: Letourneur
authors.affiliation: Inserm, U1148, LVTS, Paris Diderot University, Bichat-Claude Bernard Hospital, F-75877, Paris, France. cedric.chauvierre@inserm.fr.
authors.forename: Cédric
authors.initials: C
authors.lastname: Chauvierre
mesh_terms: D000818:Animals; D005643:Fucose / Q000032:analysis; D020723:Glucuronic Acid / Q000032:analysis; D007553:Isotope Labeling; D008297:Male; D008970:Molecular Weight; D009203:Myocardial Infarction / Q000000981:diagnostic imaging*; D015428:Myocardial Reperfusion Injury / Q000000981:diagnostic imaging; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000493:pharmacokinetics; D019275:Radiopharmaceuticals / Q000302:isolation & purification* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D013667:Technetium; D014018:Tissue Distribution; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*
chemical_list: D011134:Polysaccharides; D019275:Radiopharmaceuticals; D005643:Fucose; D013667:Technetium; D020723:Glucuronic Acid; C007789:fucoidan