title: Ultrasensitive detection of streptomycin using flow injection analysis-electrochemical quartz crystal nanobalance (FIA-EQCN) biosensor.
abstract: This work presents the development of an ultrasensitive biosensor for detection of streptomycin residues in milk samples using flow injection analysis-electrochemical quartz crystal nanobalance (FIA-EQCN) technique. Monoclonal antibody specific to streptomycin was immobilized on to the thiol modified gold quartz crystal surface. A broad dynamic range (0.3-300 ng/mL) was obtained for streptomycin with a good linearity in the range 0.3-10 ng/mL for PBS and 0.3-50 ng/mL for milk. The correlation coefficient (R(2)) of the biosensor was found to be 0.994 and 0.997 for PBS and milk respectively. Excellent recoveries were obtained from the streptomycin spiked milk samples in the range 98-99.33%, which shows the applicability of the developed biosensor in milk. The reproducibility of the developed biosensor was found satisfactory with % RSD (n=5) 0.351. A good co-relation was observed between the streptomycin recoveries measured through the developed biosensor and the commercial ELISA kit. The analytical figures of merit of the developed biosensor confirm that the developed FIA-EQCN biosensor could be very effective for low-level detection of streptomycin in milk samples.
authors.affiliation: Biosensor Lab, Department of Chemistry, BITS, Pilani - K. K. Birla Goa Campus, Zuarinagar 403726, Goa, India.
authors.forename: Geetesh K
authors.initials: GK
authors.lastname: Mishra
authors.affiliation: Biosensor Lab, Department of Chemistry, BITS, Pilani - K. K. Birla Goa Campus, Zuarinagar 403726, Goa, India.
authors.forename: Atul
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Biosensor Lab, Department of Chemistry, BITS, Pilani - K. K. Birla Goa Campus, Zuarinagar 403726, Goa, India. Electronic address: sunil17_bhand@yahoo.com.
authors.forename: Sunil
authors.initials: S
authors.lastname: Bhand
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques; D017022:Flow Injection Analysis; D006046:Gold; D008892:Milk / Q000737:chemistry*; D058525:Quartz Crystal Microbalance Techniques; D013307:Streptomycin / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal; D006046:Gold; D013307:Streptomycin
keywords: Antibiotic residue; Flow injection analysis; Milk; Quartz crystal nanobalance; Streptomycin; Ultra-sensitive detection