title: CD146 as a new marker for an increased chondroprogenitor cell sub-population in the later stages of osteoarthritis.
abstract: Cartilage-derived mesenchymal stem cells (MSCs) have been isolated with different methods. In this study lateral and medial femoral condyles were respectively collected from patients with late-stage osteoarthritis during the total knee arthroplasty. After digestion of the cartilage tissues with type II collagenase and analysis by fluorescence-activated cell sorting (FACS) with CD146, a chondroprogenitor cell sub-population were isolated and purified. The expression of other MSC-associated markers in the CD146+ chondroprogenitors was analyzed by flow cytometry. Multi-lineage differentiation capacity of CD146+ chondroprogenitors was compared with that of unsorted chondrocytes and adipose-derived MSCs (ADMSCs). Higher percentage of CD146+ chondroprogenitors isolated from the medial femoral condyles was observed than that from the lateral. CD146+ chondroprogenitors expressed high levels of MSC-specific surface antigens, and showed higher chondrogenesis capacity than ADMSCs and unsorted chondrocytes in a 3D cell pellet culture model. Thus CD146 might be a new cell surface marker for cartilage progenitor cell population in the late-stage osteoarthritis.
authors.affiliation: Department of Orthopaedics Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100730, P.R. China.
authors.forename: Xinlin
authors.initials: X
authors.lastname: Su
authors.forename: Wei
authors.initials: W
authors.lastname: Zuo
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Nan
authors.initials: N
authors.lastname: Wu
authors.forename: Pei
authors.initials: P
authors.lastname: Ma
authors.forename: Zenan
authors.initials: Z
authors.lastname: Xia
authors.forename: Chao
authors.initials: C
authors.lastname: Jiang
authors.forename: Zixing
authors.initials: Z
authors.lastname: Ye
authors.forename: Sen
authors.initials: S
authors.lastname: Liu
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Liu
authors.forename: Guangqian
authors.initials: G
authors.lastname: Zhou
authors.forename: Chao
authors.initials: C
authors.lastname: Wan
authors.forename: Guixing
authors.initials: G
authors.lastname: Qiu
mesh_terms: D000328:Adult; D000368:Aged; D019645:Arthroplasty, Replacement, Knee; D015415:Biomarkers / Q000378:metabolism; D051929:CD146 Antigen / Q000378:metabolism; D016022:Case-Control Studies; D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism / Q000473:pathology*; D018450:Disease Progression; D005260:Female; D005269:Femur / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D006801:Humans; D016130:Immunophenotyping; D007719:Knee Joint / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000378:metabolism / Q000473:pathology*; D012720:Severity of Illness Index; D013234:Stem Cells / Q000378:metabolism / Q000473:pathology*
chemical_list: D015415:Biomarkers; D051929:CD146 Antigen; C497902:MCAM protein, human
keywords: CD146; chondrogenesis; chondroprogenitors; osteoarthritis