title: Purification and characterization of recombinant Cel7A from maize seed.
abstract: The corn grain biofactory was used to produce Cel7A, an exo-cellulase (cellobiohydrolase I) from Hypocrea jecorina. The enzymatic activity on small molecule substrates was equivalent to its fungal counterpart. The corn grain-derived enzyme is glycosylated and 6 kDa smaller than the native fungal protein, likely due to more sugars added in the glycosylation of the fungal enzyme. Our data suggest that corn seed-derived cellobiohydrolase (CBH) I performs as well as or better than its fungal counterpart in releasing sugars from complex substrates such as pre-treated corn stover or wood. This recombinant protein product can enter and expand current reagent enzyme markets as well as create new markets in textile or pulp processing. The purified protein is now available commercially.
authors.affiliation: Infinite Enzymes, LLC, PO Box 2654, State University, AR, 72467, USA.
authors.forename: Nathan C
authors.initials: NC
authors.lastname: Hood
authors.forename: Kendall R
authors.initials: KR
authors.lastname: Hood
authors.forename: Susan L
authors.initials: SL
authors.lastname: Woodard
authors.forename: Shivakumar P
authors.initials: SP
authors.lastname: Devaiah
authors.forename: Tina
authors.initials: T
authors.lastname: Jeoh
authors.forename: Lisa
authors.initials: L
authors.lastname: Wilken
authors.forename: Zivko
authors.initials: Z
authors.lastname: Nikolov
authors.forename: Erin
authors.initials: E
authors.lastname: Egelkrout
authors.forename: John A
authors.initials: JA
authors.lastname: Howard
authors.forename: Elizabeth E
authors.initials: EE
authors.lastname: Hood
mesh_terms: D043366:Cellulose 1,4-beta-Cellobiosidase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005656:Fungal Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D035901:Hypocrea / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012639:Seeds / Q000201:enzymology / Q000235:genetics; D003313:Zea mays / Q000201:enzymology / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins; D043366:Cellulose 1,4-beta-Cellobiosidase