title: The step further to understand the role of cytosolic phospholipase A2 alpha and group X secretory phospholipase A2 in allergic inflammation: pilot study.
abstract: Allergens, viral, and bacterial infections are responsible for asthma exacerbations that occur with progression of airway inflammation. cPLA2 α and sPLA2X are responsible for delivery of arachidonic acid for production of eicosanoids-one of the key mediators of airway inflammation. However, cPLA2 α and sPLA2X role in allergic inflammation has not been fully elucidated. The aim of this study was to analyze the influence of rDer p1 and rFel d1 and lipopolysaccharide (LPS) on cPLA2 α expression and sPLA2X secretion in PBMC of asthmatics and in A549 cell line. PBMC isolated from 14 subjects, as well as A549 cells, were stimulated with rDer p1, rFel d1, and LPS. Immunoblotting technique was used to study the changes in cPLA2 α protein expression and ELISA was used to analyze the release of sPLA2X. PBMC of asthmatics released more sPLA2X than those from healthy controls in the steady state. rDer p1 induced more sPLA2X secretion than cPLA2 α protein expression. rFel d1 caused decrease in cPLA2 α relative expression in PBMC of asthmatics and in A549 cells. Summarizing, Der p1 and Fel d1 involve phospholipase A2 enzymes in their action. sPLA2X seems to be one of important PLA2 isoform in allergic inflammation, especially caused by house dust mite allergens.
authors.affiliation: Department of Immunopathology, Faculty of Biomedical Sciences and Postgraduate Training, Medical University of Lodz, 7/9, Zeligowskiego Street, 90-752 Lodz, Poland.
authors.forename: Ewa
authors.identifier: 0000-0001-9324-5282
authors.initials: E
authors.lastname: Pniewska
authors.affiliation: Critical Care Medicine Department, Critical Center, National Institutes of Health, Bethesda, MD 20892-1662, USA.
authors.forename: Milena
authors.identifier: 0000-0001-9710-6685
authors.initials: M
authors.lastname: Sokolowska
authors.affiliation: Department of Internal Diseases, Asthma and Allergy, Medical University of Lodz, 90-153 Lodz, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Kupryś-Lipińska
authors.affiliation: Department of Immunopathology, Faculty of Biomedical Sciences and Postgraduate Training, Medical University of Lodz, 7/9, Zeligowskiego Street, 90-752 Lodz, Poland.
authors.forename: Monika
authors.identifier: 0000-0003-2095-8681
authors.initials: M
authors.lastname: Przybek
authors.affiliation: Department of Internal Diseases, Asthma and Allergy, Medical University of Lodz, 90-153 Lodz, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Kuna
authors.affiliation: Department of Immunopathology, Faculty of Biomedical Sciences and Postgraduate Training, Medical University of Lodz, 7/9, Zeligowskiego Street, 90-752 Lodz, Poland.
authors.forename: Rafal
authors.initials: R
authors.lastname: Pawliczak
mesh_terms: D000328:Adult; D000368:Aged; D001249:Asthma / Q000201:enzymology / Q000276:immunology; D002460:Cell Line; D016207:Cytokines / Q000276:immunology; D003600:Cytosol / Q000201:enzymology / Q000276:immunology; D005260:Female; D054513:Group IV Phospholipases A2 / Q000276:immunology / Q000378:metabolism*; D054509:Group X Phospholipases A2 / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000201:enzymology* / Q000276:immunology; D008168:Lung / Q000201:enzymology* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D065631:Rhinitis, Allergic / Q000201:enzymology* / Q000276:immunology; D055815:Young Adult
chemical_list: D016207:Cytokines; D054513:Group IV Phospholipases A2; D054509:Group X Phospholipases A2