title: [Comparison of monosaccharide composition of capsular polysaccharides in Streptococcus suis serotype 1, 2, 14 and 1/2].
abstract: OBJECTIVE
  There are one-way or two-way cross-reactions among Streptococcus suis serotype 1, 2, 1/2 and 14, the reason to which was unknown.
  
  
  METHODS
  The capsular polysaccharides of serotype 14 and 1/2 were purified on Sephacryl S-300 column and identified by phenol-sulphuric acid method and dot-ELISA. The molecular weight of the serotype 14 and 1/2 capsular polysaccharides was revealed as 487.38 kDa and 512.72 kDa by high performance gel permeation chromatography, respectively.
  
  
  RESULTS
  The monosaccharide composition of serotype 14 and 1/2 capsular polysaccharides was determined as Glc/Gal/GlcNAc/Rha/Neu5Ac (1: 2.94 : 1.35 : 0.24 : 0.37) and Glc/Gal/GlcNAc/GalNAc/Rha/Neu5Ac (1 : 1.67 : 1.05 : 0.93: 0.72 : 0.7) by pre-column derivatization high performance liquid chromatography, fluorescent labeling HPLC and NMR, respectively. These were compared with the composition of serotype 1 and 2 capsular polysaccharides. Glc, GlcN, Gal and Neu5Ac was contained in the capsular polysaccharides of serotype 1, 2 14 and 1/2. But there is no prominent correlation between the monosaccharide composition and cross-reactions. The cross-reactions among them could be induced by the structure of the capsular polysaccharides and/or the other components on the cell wall.
authors.forename: Kaicheng
authors.initials: K
authors.lastname: Wang
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.forename: Chengping
authors.initials: C
authors.lastname: Lu
authors.forename: Huochun
authors.initials: H
authors.lastname: Yao
mesh_terms: D016667:Bacterial Capsules / Q000737:chemistry* / Q000378:metabolism; D009005:Monosaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D017011:Streptococcus suis / Q000737:chemistry* / Q000145:classification / Q000378:metabolism
chemical_list: D009005:Monosaccharides