title: [HPLC determination of two phenylpropanoid amides in solani melongenae radix from different cultivation regions].
abstract: A quantitative HPLC-DAD method was developed for simultaneous determination of N-trans-p-coumaroyloctopamine and N-trans-p-coumaroyltyramine in Solani Melongenae Radix from different cultivation regions in China The separation was performed on an Agilent Eclipse XDB C18 column (4.6 mm x 250 mm, 5 microm) at 30 degrees C with a gradient elution of methanol and 0.1% formic acid in water as mobile phase. The flow rate was set at 1.0 mL x min(-1) and the detection wavelength was 300 nm. The calibration curves of N-trans-p-coumaroyloctopamine and N-trans-p-coumaroyltyramine were linear over the ranges of 2.84-68.16, 3.10-74.40 mg x L(-1), and the average recoveries (n = 9) were 99.30% and 102.8%, respectively. The developed method was successfully applied for the analysis of sixteen samples from different cultivation regions in China, which indicated that the method is simple, rapid, accurate, and reliable for quality evaluation of Solani Melongenae Radix.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Yu-Fan
authors.initials: YF
authors.lastname: Gu
authors.forename: Man-Man
authors.initials: MM
authors.lastname: Li
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Su
authors.forename: Hui-Xi
authors.initials: HX
authors.lastname: Huo
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Huang
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yun-Fang
authors.initials: YF
authors.lastname: Zhao
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Tu
mesh_terms: D000577:Amides / Q000032:analysis*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry*; D019657:Solanaceae / Q000737:chemistry* / Q000145:classification
chemical_list: D000577:Amides; D004365:Drugs, Chinese Herbal