title: A specific immunological method to detect and quantify bacterial 2-substituted (1,3)-β-D-glucan.
abstract: Exopolysaccharides synthesized by lactic acid bacteria have prebiotic properties and contribute to the rheology and texture of fermented foods. Here, we have standardized an immunological method for the specific detection of 2-substituted (1,3)-β-D-glucans. The method allows direct detection and quantification of this exopolysaccharide in culture supernatants containing other mono- and poly-saccharides. Moreover, it allows specific detection of the biomolecules synthesized in vitro in enzymatic reactions. Thus, this method allows the fast identification of producing bacteria, as well as biochemical characterization of the glycosyltransferases responsible for their synthesis.
authors.affiliation: Centro de Investigaciones Biológicas, C.S.I.C., Ramiro de Maeztu 9, 28040 Madrid, Spain.
authors.forename: María Laura
authors.initials: ML
authors.lastname: Werning
authors.affiliation: Centro de Investigaciones Biológicas, C.S.I.C., Ramiro de Maeztu 9, 28040 Madrid, Spain.
authors.forename: Adrián
authors.initials: A
authors.lastname: Pérez-Ramos
authors.affiliation: Centro de Investigaciones Biológicas, C.S.I.C., Ramiro de Maeztu 9, 28040 Madrid, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Fernández de Palencia
authors.affiliation: Centro de Investigaciones Biológicas, C.S.I.C., Ramiro de Maeztu 9, 28040 Madrid, Spain.
authors.forename: María Luz
authors.initials: ML
authors.lastname: Mohedano
authors.affiliation: Departamento de Química Aplicada, Facultad de Ciencias Químicas, Box 1072, 20080 San Sebastian, Spain.
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Dueñas
authors.affiliation: Centro de Investigaciones Biológicas, C.S.I.C., Ramiro de Maeztu 9, 28040 Madrid, Spain.
authors.forename: Alicia
authors.initials: A
authors.lastname: Prieto
authors.affiliation: Centro de Investigaciones Biológicas, C.S.I.C., Ramiro de Maeztu 9, 28040 Madrid, Spain. Electronic address: plg@cib.csic.es.
authors.forename: Paloma
authors.initials: P
authors.lastname: López
mesh_terms: D001419:Bacteria / Q000737:chemistry* / Q000201:enzymology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D016695:Glycosyltransferases / Q000378:metabolism; D011509:Proteoglycans; D047071:beta-Glucans / Q000032:analysis* / Q000276:immunology
chemical_list: D011509:Proteoglycans; D047071:beta-Glucans; C010770:polysaccharide-K; D016695:Glycosyltransferases
keywords: Exopolysaccharide; Lactic acid bacteria; Prebiotic; Probiotic; β-d-Glucan