title: Improving osteoblast functions and bone formation upon BMP-2 immobilization on titanium modified with heparin.
abstract: The aim of this study was to develop bone morphogenetic protein-2 (BMP-2) immobilization on titanium (Ti) modified by heparin for improving osteoblast function in vitro and bone formation in vivo. The Ti surface was first modified with heparin and then immobilized with BMP-2 (BMP-2/Hep-Ti). The Ti and modified Ti were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and contact angle. In vitro studies demonstrated that osteoblasts cultured on BMP-2/Hep-Ti substrate increased ALP activity, calcium deposition, osteocalcin (OCN) and osteopontin (OPN) levels as compared to those cultured on Ti or BMP-2/Ti. In addition, intra-thread bone density and bone to implant contact ratio of BMP-2/Hep-Ti were significantly greater than those of Ti in the in vivo study. In conclusion, BMP-2 immobilized Ti modified heparin implants seemed to be a suitable delivery system for BMP-2 to improve osteoblast functions and new bone formation at the defect area around an implant.
authors.affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical Center, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Kim
authors.affiliation: Department of Prosthodontics, Dental Research Institute, School of Dentistry, Pusan National University, Yang San 626-787, Republic of Korea.
authors.forename: Chang-Seop
authors.initials: CS
authors.lastname: Kim
authors.affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical Center, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.
authors.forename: Young-Pil
authors.initials: YP
authors.lastname: Yun
authors.affiliation: Institute of Cell & Tissue Engineering, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea.
authors.forename: Dae Hyeok
authors.initials: DH
authors.lastname: Yang
authors.affiliation: Division of Bio-Imaging, Chuncheon Center, Korea Basic Science Institute, 192-1, Hyoja 2-dong, Chuncheon 200-701, Gangwon-do, Republic of Korea.
authors.forename: Kyeongsoon
authors.initials: K
authors.lastname: Park
authors.affiliation: Department of Veterinary Surgery, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Se Eun
authors.initials: SE
authors.lastname: Kim
authors.affiliation: Department of Prosthodontics, Dental Research Institute, School of Dentistry, Pusan National University, Yang San 626-787, Republic of Korea.
authors.forename: Chang-Mo
authors.initials: CM
authors.lastname: Jeong
authors.affiliation: Department of Prosthodontics, Dental Research Institute, School of Dentistry, Pusan National University, Yang San 626-787, Republic of Korea. Electronic address: huhjb@pusan.ac.kr.
authors.forename: Jung-Bo
authors.initials: JB
authors.lastname: Huh
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D004285:Dogs; D006493:Heparin / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D055767:Immobilized Proteins / Q000378:metabolism* / Q000494:pharmacology; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D056951:Photoelectron Spectroscopy / Q000379:methods; D014025:Titanium / Q000378:metabolism* / Q000494:pharmacology
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D055767:Immobilized Proteins; D006493:Heparin; D014025:Titanium
keywords: 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) (PubChem CID:2723939); 2-(N-morpholino)-ethanesulfonic acid (Pubchem CID:4478249); 4′,6-Diamidino-2-phenylindole (PubChem CID:2954); Bone formation; Bone morphogenetic protein-2 (BMP-2); Dopamine hydrochloride (PubChem CID:65340); Heparin; Magnesium chloride (PubChem CID:24584); N-hydroxysuccinimide (PubChem CID:80170); Osteoblast function; Phenylmethylsulfonyl fluoride (PubChem CID:4784); Sodium azide (PubChem CID:33557); Sodium chloride (PubChem CID:5234); Titanium (Ti); Tris-hydrochloride (PubChem CID:10997301)