title: Downregulation of serum brain specific microRNA is associated with inflammation and infarct volume in acute ischemic stroke.
abstract: Cerebral ischemic injury activates a robust inflammatory response, exacerbating neurological deficit. Several brain specific microRNA (miRNA) molecules have been reported to mediate functioning of the immune system, referred to as NeurimmiR. We aimed to explore possible associations between serum miRNA levels and stroke severity and their involvement in the regulation of inflammatory responses after stroke. Blood samples were obtained from 31 patients with acute ischemic stroke and 11 healthy controls. We evaluated infarct volume using diffusion weighted imaging and neurological deficit using the National Institutes of Health Stroke Scale. Serum levels of three NeurimmiR, miR-124, miR-9 and miR-219 were detected by real-time polymerase chain reaction and serum levels of metalloproteinase-9 (MMP-9), a proinflammation marker in brain injury, were examined by enzyme-linked immunosorbent assay. We found that serum miR-124 was significantly decreased within 24 hours after stroke onset and serum miR-9 was decreased in patients with larger stroke. There were no significant changes in serum miR-219. Both serum miR-124 and miR-9 levels within 24 hours were negatively correlated with infarct volume and plasma high-sensitivity C-reactive protein levels. All three NeurimmiR negatively correlated with MMP-9 levels. Our preliminary findings indicate that serum miR-124, miR-9 and miR-219 are suppressed in acute ischemic stroke thus facilitating neuroinflammation and brain injury.
authors.affiliation: Department of Neurology, Zhongnan Hospital, Wuhan University, Donghu Road 169, Wuhan 430071, China; Department of Neurology, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Neurology, Zhongnan Hospital, Wuhan University, Donghu Road 169, Wuhan 430071, China. Electronic address: wdsjkx@163.com.
authors.forename: Junjian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Immunology, School of Basic Medical Science, Wuhan University, Wuhan, China.
authors.forename: Rongfei
authors.initials: R
authors.lastname: Han
authors.affiliation: Department of Neurology, Zhongnan Hospital, Wuhan University, Donghu Road 169, Wuhan 430071, China.
authors.forename: Hanxing
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Neurology, Zhongnan Hospital, Wuhan University, Donghu Road 169, Wuhan 430071, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Sun
authors.affiliation: Department of Neurology, Zhongnan Hospital, Wuhan University, Donghu Road 169, Wuhan 430071, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D001921:Brain / Q000378:metabolism / Q000473:pathology; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D060888:Real-Time Polymerase Chain Reaction; D020521:Stroke / Q000097:blood / Q000235:genetics* / Q000473:pathology; D014481:United States
chemical_list: D015415:Biomarkers; C509207:MIRN124 microRNA, human; C578248:MIRN219 microRNA, human; C541874:MIRN92 microRNA, human; D035683:MicroRNAs
keywords: Acute ischemic stroke; High-sensitivity C-reactive protein; Infarct volume; Metalloproteinase-9; Serum brain specific miRNA