title: Inhibitory effects of Cnidium monnieri fruit extract on pulmonary inflammation in mice induced by cigarette smoke condensate and lipopolysaccharide.
abstract: The aim of this study was to investigate the inhibitory effect of Cnidium monnieri fruit (CM) extracts on pulmonary inflammation induced in mice by cigarette smoke condensate (CSC) and lipopolysaccharide (LPS). Pulmonary inflammation was induced by intratracheal instillation of LPS and CSC five times within 12 days. CM extract was administered orally at a dose of 50 or 200 mg·kg(-1). The number of inflammatory cells in the bronchoalveolar lavage fluid was counted using a fluorescence activated cell sorter. Inflammatory mediator levels were determined by enzyme-linked immunosorbent assay. The administration of LPS and CSC exacerbated airway hyper-responsiveness (AHR) and induced an accumulation of inflammatory cells and mediators, and led to histological changes. However, these responses are modulated by treatment with CM, and the treatment with CM extract produces similar or more extensive results than the treatment with cyclosporin A (CSA). CM extract may have an inhibitory effect on pulmonary inflammation related with chronic obstructive pulmonary disease.
authors.affiliation: Department of Industrial Plant Science & Technology, Chungbuk National University, Cheongju, 361-763, Republic of Korea.
authors.forename: Ho-Geun
authors.initials: HG
authors.lastname: Kwak
authors.affiliation: Department of Industrial Plant Science & Technology, Chungbuk National University, Cheongju, 361-763, Republic of Korea. Electronic address: Heungbin@chungbuk.ac.kr.
authors.forename: Heung-Bin
authors.initials: HB
authors.lastname: Lim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001992:Bronchoalveolar Lavage Fluid; D031048:Cnidium; D005260:Female; D005638:Fruit; D008070:Lipopolysaccharides; D008807:Mice, Inbred BALB C; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D011014:Pneumonia / Q000139:chemically induced / Q000188:drug therapy*; D029424:Pulmonary Disease, Chronic Obstructive / Q000188:drug therapy / Q000473:pathology; D012906:Smoke / Q000009:adverse effects*; D012907:Smoking / Q000009:adverse effects*; D062789:Tobacco Products / Q000009:adverse effects
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D010936:Plant Extracts; D012906:Smoke
keywords: Chronic obstructive pulmonary disease; Cigarette smoke condensate; Cnidium monnieri fruits; Lipopolysaccharide; Pulmonary inflammation