title: Laser line scanning for fluorescence reflectance imaging: a phantom study and in vivo validation of the enhancement of contrast and resolution.
abstract: Intraoperative fluorescence imaging in reflectance geometry is an attractive imaging modality to noninvasively monitor fluorescence-targeted tumors. In some situations, this kind of imaging suffers from poor resolution due to the diffusive nature of photons in tissue. The objective of the proposed technique is to tackle this limitation. It relies on the scanning of the medium with a laser line illumination and the acquisition of images at each position of excitation. The detection scheme proposed takes advantage of the stack of images acquired to enhance the resolution and the contrast of the final image. The experimental protocol is described to fully understand why we overpass the classical limits and validate the scheme on tissue-like phantoms and in vivo with a preliminary testing. The results are compared with those obtained with a classical wide-field illumination.
authors.affiliation: Université Grenoble Alpes, 38000 Grenoble, FrancebCEA, LETI, Minatec Campus, 38054 Grenoble, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Fantoni
authors.affiliation: Université Grenoble Alpes, 38000 Grenoble, FrancebCEA, LETI, Minatec Campus, 38054 Grenoble, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Hervé
authors.affiliation: Université Grenoble Alpes, 38000 Grenoble, FrancebCEA, LETI, Minatec Campus, 38054 Grenoble, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Poher
authors.affiliation: Harvard Medical School, Beth Israel Deaconess Medical Center, Department of Medicine, Boston, Massachusetts 02215, United States.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Gioux
authors.affiliation: GIPSA Lab, UMR 5216 CNRS-Grenoble INP-Université Joseph Fourier-Université Stendhal, 11 rue des Mathématiques, 38402 Saint Martin D'Heres, France.
authors.forename: Jérôme I
authors.initials: JI
authors.lastname: Mars
authors.affiliation: Université Grenoble Alpes, 38000 Grenoble, FrancebCEA, LETI, Minatec Campus, 38054 Grenoble, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Dinten
mesh_terms: D000818:Animals; D005260:Female; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D061848:Optical Imaging / Q000379:methods*; D019047:Phantoms, Imaging; D015203:Reproducibility of Results