title: Silencing of STRN4 suppresses the malignant characteristics of cancer cells.
abstract: The striatin family of proteins, comprising STRN, STRN3 and STRN4, are multidomain-containing proteins that associate with additional proteins to form a large protein complex. We previously reported that STRN4 directly associated with protein kinases, such as MINK1, TNIK and MAP4K4, which are associated with tumor suppression or tumor progression. However, it remains unclear whether STRN4 is associated with tumor progression. In this report, we examined the role that STRN4 plays in cancer malignancy. We show that depletion of STRN4 suppresses proliferation, migration, invasion and the anchorage-independent growth of cancer cells. In addition, STRN4 knockdown increases the sensitivity of pancreatic cancer cells to gemcitabine. Finally, we show that STRN4 knockdown suppresses the proliferation and metastasis of cancer cells in mice. Our results demonstrate a possible role of STRN4 in tumor progression.
authors.affiliation: Department of Gastroenterology and Hepatology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Meihong
authors.initials: M
authors.lastname: Wong
authors.forename: Toshinori
authors.initials: T
authors.lastname: Hyodo
authors.forename: Eri
authors.initials: E
authors.lastname: Asano
authors.forename: Kohei
authors.initials: K
authors.lastname: Funasaka
authors.forename: Ryoji
authors.initials: R
authors.lastname: Miyahara
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Hirooka
authors.forename: Hidemi
authors.initials: H
authors.lastname: Goto
authors.forename: Michinari
authors.initials: M
authors.lastname: Hamaguchi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Senga
mesh_terms: D000818:Animals; D023102:Anoikis; D002148:Calmodulin-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D055785:Gene Knockdown Techniques; D045325:HCT116 Cells; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis / Q000235:genetics / Q000473:pathology*; D009374:Neoplasms, Experimental / Q000235:genetics / Q000473:pathology*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000473:pathology*; D034741:RNA, Small Interfering / Q000378:metabolism; D000093542:Gemcitabine
chemical_list: D002148:Calmodulin-Binding Proteins; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering; C000595263:STRN4 protein, human; C000595264:STRN4 protein, mouse; C411649:zinedin; D003841:Deoxycytidine; D000093542:Gemcitabine
keywords: Anoikis; STRIPAK; STRN4; invasion; migration Striatin