title: In vivo visualization of the development of the enteric nervous system using a Tg(-8.3bphox2b:Kaede) transgenic zebrafish.
abstract: The phox2b gene encodes a transcription factor that is expressed in the developing enteric nervous system (ENS). An enhancer element has been identified in the zebrafish phox2b locus that can drive tissue specific expression of reporter genes in enteric neuron precursor cells. We have generated a transgenic zebrafish line in which the Kaede fluorescent protein is under the control of this phox2b enhancer. This line has stable expression of the Kaede protein in enteric neuron precursor cells over three generations. To demonstrate the utility of this line we compared the migration and division rates of enteric neuron precursor cells in wild type and the zebrafish ENS mutant lessen.
authors.affiliation: Department of Biology, Emory University, Atlanta, Georgia.
authors.forename: Colin
authors.initials: C
authors.lastname: Harrison
authors.forename: Tara
authors.initials: T
authors.lastname: Wabbersen
authors.forename: Iain T
authors.initials: IT
authors.lastname: Shepherd
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004742:Enhancer Elements, Genetic; D017615:Enteric Nervous System / Q000196:embryology / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D009928:Organ Specificity; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D018398:Homeodomain Proteins; D008164:Luminescent Proteins; C109220:NBPhox protein; D014157:Transcription Factors; D029961:Zebrafish Proteins
keywords: Kaede; enteric nervous system; in vivo; med24; phox2b