title: Seasonal distribution of potentially pathogenic Acanthamoeba species from drinking water reservoirs in Taiwan.
abstract: In order to detect the presence/absence of Acanthamoeba along with geographical variations, water quality variations and seasonal change of Acanthamoeba in Taiwan was investigated by 18S ribosomal RNA (rRNA) gene TaqMan quantitative real-time PCR. Samples were collected quarterly at 19 drinking water reservoir sites from November 2012 to August 2013. Acanthamoeba was detected in 39.5 % (30/76) of the water sample, and the detection rate was 63.2 % (12/19) from samples collected in autumn. The average concentration of Acanthamoeba was 3.59 × 10(4) copies/L. For geographic distribution, the detection rate for Acanthamoeba at the northern region was higher than the central and southern regions in all seasons. Results of Spearman rank test revealed that heterotrophic plate count (HPC) had a negative correlation (R = -0.502), while dissolved oxygen (DO) had a positive correlation (R = 0.463) in summer. Significant differences were found only between the presence/absence of Acanthamoeba and HPC in summer (Mann-Whitney U test, P < 0.05). T2 and T4 genotypes of Acanthamoeba were identified, and T4 was the most commonly identified Acanthamoeba genotypes. The presence of Acanthamoeba in reservoirs presented a potential public health threat and should be further examined.
authors.affiliation: Department of Earth and Environmental Sciences, National Chung Cheng University, Chiayi, Taiwan, Republic of China.
authors.forename: Po-Min
authors.initials: PM
authors.lastname: Kao
authors.forename: Bing-Mu
authors.initials: BM
authors.lastname: Hsu
authors.forename: Tsui-Kang
authors.initials: TK
authors.lastname: Hsu
authors.forename: Jorn-Hon
authors.initials: JH
authors.lastname: Liu
authors.forename: Hsiang-Yu
authors.initials: HY
authors.lastname: Chang
authors.forename: Wen-Tsai
authors.initials: WT
authors.lastname: Ji
authors.forename: Kai-Jiun
authors.initials: KJ
authors.lastname: Tzeng
authors.forename: Shih-Wei
authors.initials: SW
authors.lastname: Huang
authors.forename: Yu-Li
authors.initials: YL
authors.lastname: Huang
mesh_terms: D000048:Acanthamoeba / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D060766:Drinking Water / Q000469:parasitology*; D004784:Environmental Monitoring / Q000379:methods / Q000706:statistics & numerical data*; D005838:Genotype; D005843:Geography; D011156:Population Density; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012621:Seasons; D018709:Statistics, Nonparametric; D013624:Taiwan; D060753:Water Quality / Q000592:standards*
chemical_list: D060766:Drinking Water; D012337:RNA, Ribosomal, 18S