title: The role of mitochondria in T-2 toxin-induced human chondrocytes apoptosis.
abstract: T-2 toxin, a mycotoxin produced by Fusarium species, has been shown to cause diverse toxic effects in animals and is also a possible pathogenic factor of Kashin-Beck disease (KBD). The role of mitochondria in KBD is recognized in our recent research. The aim of this study was to evaluate the role of mitochondria in T-2 toxin-induced human chondrocytes apoptosis to understand the pathogenesis of KBD. T-2 toxin decreased chondrocytes viabilities in concentration- and time-dependent manners. Exposure to T-2 toxin can reduce activities of mitochondrial complexes III, IV and V, ΔΨm and the cellular ATP, while intracellular ROS increased following treatment with T-2 toxin. Furthermore, mitochondrial cytochrome c release, caspase-9 and 3 activation and chondrocytes apoptosis were also obviously observed. Interestingly, Selenium (Se) can partly block T-2 toxin -induced mitochondria dysfunction, oxidative damage and chondrocytes apoptosis. These results suggest that the effect of T-2 toxin on human chondrocytes apoptosis may be mediated by a mitochondrial pathway, which is highly consistent with the chondrocytes changes in KBD.
authors.affiliation: School of Public Health, Science Health Center of Xi'an Jiaotong University, Key Laboratory of Environment and Genes Related Diseases, Ministry of Education, Xi'an, Shaanxi, PR China; Department of Orthopedics Surgery, Ningbo No.2 Hospital, Ningbo, Zhejiang, PR China.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Ningbo First Hospital, Ningbo, Zhejiang, PR China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Public Health, Science Health Center of Xi'an Jiaotong University, Key Laboratory of Environment and Genes Related Diseases, Ministry of Education, Xi'an, Shaanxi, PR China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Orthopedics Surgery, Ningbo No.2 Hospital, Ningbo, Zhejiang, PR China.
authors.forename: Qingjiang
authors.initials: Q
authors.lastname: Pang
authors.affiliation: School of Public Health, Science Health Center of Xi'an Jiaotong University, Key Laboratory of Environment and Genes Related Diseases, Ministry of Education, Xi'an, Shaanxi, PR China.
authors.forename: Shixun
authors.initials: S
authors.lastname: Wu
authors.affiliation: School of Public Health, Science Health Center of Xi'an Jiaotong University, Key Laboratory of Environment and Genes Related Diseases, Ministry of Education, Xi'an, Shaanxi, PR China.
authors.forename: Cuiyan
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Orthopaedics Surgery, The Xi'an Red Cross Hospital, Xi'an, Shaanxi, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.affiliation: Department of Cellular and Structural Biology, University of Texas Health Sciences Center at San Antonio, San Antonio, Texas, United States of America.
authors.forename: Yidong
authors.initials: Y
authors.lastname: Bai
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000276:immunology*; D002358:Cartilage, Articular / Q000166:cytology; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects*; D019902:Chondrocytes / Q000276:immunology*; D002950:Citrate (si)-Synthase / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D014450:Electron Transport Complex III / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D004789:Enzyme Activation; D005670:Fusarium / Q000472:pathogenicity; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D057767:Kashin-Beck Disease / Q000276:immunology / Q000473:pathology; D008875:Middle Aged; D008928:Mitochondria / Q000276:immunology / Q000473:pathology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012643:Selenium / Q000494:pharmacology; D013605:T-2 Toxin / Q000037:antagonists & inhibitors / Q000276:immunology / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D045304:Cytochromes c; D003576:Electron Transport Complex IV; D002950:Citrate (si)-Synthase; D053148:Caspase 3; D053453:Caspase 9; D014450:Electron Transport Complex III; D005978:Glutathione; D012643:Selenium; D013605:T-2 Toxin