title: Centriole maturation requires regulated Plk1 activity during two consecutive cell cycles.
abstract: Newly formed centrioles in cycling cells undergo a maturation process that is almost two cell cycles long before they become competent to function as microtubule-organizing centers and basal bodies. As a result, each cell contains three generations of centrioles, only one of which is able to form cilia. It is not known how this long and complex process is regulated. We show that controlled Plk1 activity is required for gradual biochemical and structural maturation of the centrioles and timely appendage assembly. Inhibition of Plk1 impeded accumulation of appendage proteins and appendage formation. Unscheduled Plk1 activity, either in cycling or interphase-arrested cells, accelerated centriole maturation and appendage and cilia formation on the nascent centrioles, erasing the age difference between centrioles in one cell. These findings provide a new understanding of how the centriole cycle is regulated and how proper cilia and centrosome numbers are maintained in the cells.
authors.affiliation: Laboratory of Protein Dynamics and Signaling, Center for Cancer Research-Frederick, National Cancer Institute, National Institutes of Health, Frederick, MD 21702.
authors.forename: Dong
authors.initials: D
authors.lastname: Kong
authors.affiliation: Laboratory of Protein Dynamics and Signaling, Center for Cancer Research-Frederick, National Cancer Institute, National Institutes of Health, Frederick, MD 21702.
authors.forename: Veronica
authors.initials: V
authors.lastname: Farmer
authors.affiliation: Laboratory of Protein Dynamics and Signaling, Center for Cancer Research-Frederick, National Cancer Institute, National Institutes of Health, Frederick, MD 21702.
authors.forename: Anil
authors.initials: A
authors.lastname: Shukla
authors.affiliation: Laboratory of Protein Dynamics and Signaling, Center for Cancer Research-Frederick, National Cancer Institute, National Institutes of Health, Frederick, MD 21702.
authors.forename: Jana
authors.initials: J
authors.lastname: James
authors.affiliation: Laboratory of Protein Dynamics and Signaling, Center for Cancer Research-Frederick, National Cancer Institute, National Institutes of Health, Frederick, MD 21702.
authors.forename: Richard
authors.initials: R
authors.lastname: Gruskin
authors.affiliation: Laboratory of Protein Dynamics and Signaling, Center for Cancer Research-Frederick, National Cancer Institute, National Institutes of Health, Frederick, MD 21702.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Kiriyama
authors.affiliation: Laboratory of Protein Dynamics and Signaling, Center for Cancer Research-Frederick, National Cancer Institute, National Institutes of Health, Frederick, MD 21702 jadranka.loncarek@nih.gov.
authors.forename: Jadranka
authors.initials: J
authors.lastname: Loncarek
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000502:physiology*; D002502:Centrioles / Q000201:enzymology* / Q000502:physiology / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D008868:Microtubule Proteins / Q000378:metabolism; D008938:Mitosis; D017346:Protein Serine-Threonine Kinases / Q000502:physiology*; D021381:Protein Transport; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D000097869:Polo-Like Kinase 1
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; D008868:Microtubule Proteins; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D000097869:Polo-Like Kinase 1; C524694:CEP164 protein, human; C095245:FBF1 protein, human