title: Visceral granulomas in farmed large yellow croaker,  Larimichthys crocea (Richardson), caused by a bacterial  pathogen, Pseudomonas plecoglossicida.
abstract: An enzootic disease characterized by granulomas in internal organs occurred in cage-farmed large yellow croaker, Larimichthys crocea (Richardson), in April and November 2010, in Ningbo, Zhejiang Province. One bacterial strain, named XSDHY-P, was isolated from the diseased fish and identified by biochemical characterization, fatty acid methyl ester (FAME) analysis and multilocus sequence analysis (MLSA). According to the results obtained from the biochemical tests, FAME analysis and phylogenetic analysis derived from 16S ribosomal RNA, gyrB, oprF, oprI, oprL and rpoD gene sequencing, the bacterial isolate, XSDHY-P, was identified as Pseudomonas plecoglossicida. Moreover, lethal dose, 50% trials were carried out to demonstrate the virulence of XSDHY-P in large yellow croaker when administered at 2.13 9 105 colony-forming units per fish. Visceral granulomas were found in the experimentally infected fish as well as in the naturally infected fish, indicating that P. plecoglossicida is another bacterial pathogen that causes granulomatosis in L. crocea.
authors.forename: J T
authors.initials: JT
authors.lastname: Zhang
authors.forename: S M
authors.initials: SM
authors.lastname: Zhou
authors.forename: S W
authors.initials: SW
authors.lastname: An
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: G L
authors.initials: GL
authors.lastname: Wang
mesh_terms: D000818:Animals; D005393:Fish Diseases / Q000382:microbiology* / Q000401:mortality; D006099:Granuloma / Q000382:microbiology / Q000401:mortality / Q000662:veterinary*; D058885:Multilocus Sequence Typing; D010473:Perciformes / Q000502:physiology*; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S