title: Caffeine suppresses exercise-enhanced long-term and location memory in middle-aged rats: Involvement of hippocampal Akt and CREB signaling.
abstract: The cognitive function decline is closely related with brain changes generated by age. The ability of caffeine and exercise to prevent memory impairment has been reported in animal models and humans. The purpose of the present study was to investigate whether swimming exercise and caffeine administration enhance memory in middle-aged Wistar rats. Male Wistar rats (18months) received caffeine at a dose of 30mg/kg, 5days per week by a period of 4weeks. Animals were subjected to swimming training with a workload (3% of body weight, 20min per day for 4weeks). After 4weeks, the object recognition test (ORT) and the object location test (OLT) were performed. The results of this study demonstrated that caffeine suppressed exercise-enhanced long-term (ORT) and spatial (OLT) memory in middle-aged and this effect may be related to a decrease in hippocampal p-CREB signaling. This study also provided evidence that the effects of this protocol on memory were not accompanied by alterations in the levels of activated Akt. The [(3)H] glutamate uptake was reduced in hippocampus of rats administered with caffeine and submitted to swimming protocol.
authors.affiliation: Laboratório de Síntese, Reatividade e Avaliação Farmacológica e Toxicológica de Organocalcogênios, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria CEP 97105-900, Rio Grande do Sul, Brazil.
authors.forename: José L
authors.initials: JL
authors.lastname: Cechella
authors.affiliation: Laboratório de Síntese, Reatividade e Avaliação Farmacológica e Toxicológica de Organocalcogênios, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria CEP 97105-900, Rio Grande do Sul, Brazil.
authors.forename: Marlon R
authors.initials: MR
authors.lastname: Leite
authors.affiliation: Laboratório de Síntese, Reatividade e Avaliação Farmacológica e Toxicológica de Organocalcogênios, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria CEP 97105-900, Rio Grande do Sul, Brazil.
authors.forename: Juliana T
authors.initials: JT
authors.lastname: da Rocha
authors.affiliation: Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Dobrachinski
authors.affiliation: Laboratório de Síntese, Reatividade e Avaliação Farmacológica e Toxicológica de Organocalcogênios, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria CEP 97105-900, Rio Grande do Sul, Brazil; Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Bibiana M
authors.initials: BM
authors.lastname: Gai
authors.affiliation: Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Félix A A
authors.initials: FA
authors.lastname: Soares
authors.affiliation: Laboratório de Bioquímica do Exercício, Centro de Educação Física e Desportos, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil; Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Temuco, Chile.
authors.forename: Guilherme
authors.initials: G
authors.lastname: Bresciani
authors.affiliation: Laboratório de Bioquímica do Exercício, Centro de Educação Física e Desportos, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Luiz F F
authors.initials: LF
authors.lastname: Royes
authors.affiliation: Laboratório de Síntese, Reatividade e Avaliação Farmacológica e Toxicológica de Organocalcogênios, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria CEP 97105-900, Rio Grande do Sul, Brazil. Electronic address: gzeni@quimica.ufsm.br.
authors.forename: Gilson
authors.initials: G
authors.lastname: Zeni
mesh_terms: D000375:Aging / Q000378:metabolism / Q000523:psychology; D000818:Animals; D002110:Caffeine / Q000008:administration & dosage / Q000009:adverse effects*; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008569:Memory Disorders / Q000209:etiology / Q000378:metabolism / Q000523:psychology; D057567:Memory, Long-Term / Q000187:drug effects* / Q000502:physiology*; D005082:Physical Exertion; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D065852:Spatial Memory / Q000187:drug effects* / Q000502:physiology*; D013550:Swimming
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D002110:Caffeine; D018698:Glutamic Acid; D051057:Proto-Oncogene Proteins c-akt
keywords: Akt; CREB; Caffeine; Exercise; Memory; Middle-aged