title: A cell-based model of bone remodeling for identifying activity of icarrin in the treatment of osteoporosis.
abstract: The activity of icarrin (a flavonoid from Herba epimedii) was investigated in the regulation of bone remodeling, a process coupled by osteoblast-mediated bone forming and osteoclast-mediated bone resorption. By directly co-culturing mouse bone marrow stromal cells and mouse preosteoclastic RAW264.7, and transwell co-culturing rat ovarian follicular granulosa cells (FGC), a 30 % increase in alkaline phosphatase (ALP) activity and 25 % increase in estradiol level occurred. Compared with the antiresorptive drug, alendronate, and an anabolic drug, PTH1-34, icarrin possessed all of the positive effects on the co-culture by increasing ALP activity, estradiol production and decreasing tartrate-resistant acid phosphatase activity. A similar action of icarrin occurred on co-culture of mesenchymal stem cells, mouse peripheral blood mononuclear cells, and FGC. Overall, by using a co-cultured cell-based in vitro screening assay, icarrin is suggested as a new class of dual-action therapeutic agent for osteoporosis.
authors.affiliation: College (Institute) of Integrative Medicine, Dalian Medical University, Dalian, 116044, China.
authors.forename: Yan-Qiu
authors.initials: YQ
authors.lastname: Liu
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Han
authors.forename: Tiegang
authors.initials: T
authors.lastname: Liu
authors.forename: Meng-Chun
authors.initials: MC
authors.lastname: Cheng
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Xiao
mesh_terms: D019386:Alendronate / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D016723:Bone Remodeling / Q000187:drug effects*; D002460:Cell Line; D034181:Epimedium / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry* / Q000494:pharmacology*; D005960:Glucosides / Q000737:chemistry* / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008954:Models, Biological; D010024:Osteoporosis / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology
chemical_list: D005419:Flavonoids; D005960:Glucosides; D010281:Parathyroid Hormone; D010936:Plant Extracts; C000600955:icarrin; D019386:Alendronate