title: Conformational cycle and ion-coupling mechanism of the Na+/hydantoin transporter Mhp1.
abstract: Ion-dependent transporters of the LeuT-fold couple the uptake of physiologically essential molecules to transmembrane ion gradients. Defined by a conserved 5-helix inverted repeat that encodes common principles of ion and substrate binding, the LeuT-fold has been captured in outward-facing, occluded, and inward-facing conformations. However, fundamental questions relating to the structural basis of alternating access and coupling to ion gradients remain unanswered. Here, we used distance measurements between pairs of spin labels to define the conformational cycle of the Na(+)-coupled hydantoin symporter Mhp1 from Microbacterium liquefaciens. Our results reveal that the inward-facing and outward-facing Mhp1 crystal structures represent sampled intermediate states in solution. Here, we provide a mechanistic context for these structures, mapping them into a model of transport based on ion- and substrate-dependent conformational equilibria. In contrast to the Na(+)/leucine transporter LeuT, our results suggest that Na(+) binding at the conserved second Na(+) binding site does not change the energetics of the inward- and outward-facing conformations of Mhp1. Comparative analysis of ligand-dependent alternating access in LeuT and Mhp1 lead us to propose that different coupling schemes to ion gradients may define distinct conformational mechanisms within the LeuT-fold class.
authors.affiliation: Chemical and Physical Biology Program, Vanderbilt University, Nashville, TN 37230; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232; and.
authors.forename: Kelli
authors.initials: K
authors.lastname: Kazmier
authors.affiliation: Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232; and.
authors.forename: Shruti
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637.
authors.forename: Shahidul M
authors.initials: SM
authors.lastname: Islam
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637.
authors.forename: Benoît
authors.initials: B
authors.lastname: Roux
authors.affiliation: Chemical and Physical Biology Program, Vanderbilt University, Nashville, TN 37230; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232; and hassane.mchaourab@vanderbilt.edu.
authors.forename: Hassane S
authors.initials: HS
authors.lastname: Mchaourab
mesh_terms: D000192:Actinomycetales / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D018360:Crystallography, X-Ray; D005110:Extracellular Space / Q000378:metabolism; D006827:Hydantoins / Q000378:metabolism*; D017136:Ion Transport; D007477:Ions; D008024:Ligands; D008958:Models, Molecular; D011487:Protein Conformation; D012964:Sodium / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D006827:Hydantoins; D007477:Ions; D008024:Ligands; D012964:Sodium
keywords: DEER; EPR; Na+-coupled symport; conformational dynamics; transport mechanism