title: Endostatin and cathepsin-V in bronchoalveolar lavage fluid of patients with pulmonary sarcoidosis.
abstract: Recently, it has been reported that lack of cathepsins prevent the development of lung granulomas in a mouse model of Besnier-Boeck-Schaumann (BBS) disease, sarcoidosis. There is no data about cathepsin V (Cath V) in bronchoalveolar lavage fluid (BALF) in humans. Endostatin is a novel inhibitor of lung epithelial cells. The role of this protein in BBS is not determined. The aim of this study was to evaluate the concentration of endostatin, Cath V, and IL-18 in BALF of BBS patients. We studied 22 BBS patients (Stage 2). The control group consisted of 20 healthy subjects. Cath V concentration was lower in BBS than in healthy group (16.03±8.60 vs. 32.25±21.90 pg/ml, p=0.004). Both endostatin and IL-18 levels were higher in BBS than in the control group (0.88±0.30 vs. 0.29±0.04 ng/ml, p=0.028; 40.37±31.60 vs. 14.61±1.30 pg/ml, p=0.007, respectively). In BBS there were correlations between the levels of endostatin and IL-18 (r=0.74, p=0.001) as well as endostatin and DLCO (diffusing capacity for carbon monoxide) (r=-0.6, p=0.013). Receiver-operating characteristic (ROC) curves were applied to find the cut-off for the BALF levels of Cath V, endostatin, and IL-18. We conclude that Cath V and endostatin may represent an index of pulmonary sarcoidosis activity.
authors.affiliation: Department of Lung Diseases, Medical University of Bialystok, 14 Zurawia St., 15-540, Bialystok, Poland, wojciechnaumnik@gmail.com.
authors.forename: W
authors.initials: W
authors.lastname: Naumnik
authors.forename: M
authors.initials: M
authors.lastname: Ossolińska
authors.forename: I
authors.initials: I
authors.lastname: Płońska
authors.forename: E
authors.initials: E
authors.lastname: Chyczewska
authors.forename: J
authors.initials: J
authors.lastname: Nikliński
mesh_terms: D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002403:Cathepsins / Q000032:analysis*; D003546:Cysteine Endopeptidases / Q000032:analysis*; D043169:Endostatins / Q000032:analysis*; D005260:Female; D006801:Humans; D020382:Interleukin-18 / Q000032:analysis; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D017565:Sarcoidosis, Pulmonary / Q000378:metabolism*
chemical_list: D043169:Endostatins; D020382:Interleukin-18; D002403:Cathepsins; D003546:Cysteine Endopeptidases; C117627:CTSV protein, human