title: Chemical communication between cardiac cells is disrupted by high glucose: implications for the diabetic heart.
abstract: The influence of high glucose solution on the chemical communication between cardiac cells was investigated in cell pairs isolated from the left ventricle of adult Wistar Kyoto rats. For this, Lucifer Yellow CH was dialyzed into one cell of the pair using the whole cell clamp technique, and the diffusion of dye in the dialyzed as well as in non-dialyzed cell, was followed by measuring the intensity of fluorescence in both cells as a function of time. The results indicated that: 1) high glucose solution (25 mM) disrupted chemical communication between cardiac cells; 2) the effect of high glucose solution was reduced by Bis-1 (10(-9)M) which is a PKC inhibitor, and by enalapril (10(-9)M); 3) intracellular dialysis of Ang II (100 nM) also caused dye uncoupling; 4) calculation of gap junction permeability (Pj) (cm/s) indicated a value of 3 ± 0.07 × 10(-5)cm/s; n=32; (6 animals) for the controls and 0.4 ± 0.86 × 10(-6)cm/s; n=35 (6 animals) (P<0.05) for cells incubated with high glucose solution for 24h; 5) measurements of Pj for cell pairs treated with hypertonic solution plus Bis-1 (10(-9)M) or enalapril maleate (10(-9)M) showed no significant change of Pj (P>0.05).
  
  
  CONCLUSIONS
  high glucose (25 mM) disrupts chemical communication between cardiac cells-an effect highly dependent on PKC activation. The possible role of enhanced intracellular Ang II levels induced by high glucose on the disruption of chemical communication was discussed as well as the possible implications of these findings for the diabetic heart.
authors.affiliation: School of Medicine, Medical Sciences Campus, UPR, San Juan, PR 00936, USA. Electronic address: walmor.de-mello@upr.edu.
authors.forename: Walmor C
authors.initials: WC
authors.lastname: De Mello
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000187:drug effects*; D002478:Cells, Cultured; D003920:Diabetes Mellitus; D017629:Gap Junctions / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D006352:Heart Ventricles / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D011921:Rats, Inbred WKY; D013549:Sweetening Agents / Q000494:pharmacology
chemical_list: D013549:Sweetening Agents; D011493:Protein Kinase C; D005947:Glucose
keywords: Chemical communication; Heart cells; High glucose