title: Intraoperative cerebral blood flow imaging of rodents.
abstract: Intraoperative monitoring of cerebral blood flow (CBF) is of interest to neuroscience researchers, which offers the assessment of hemodynamic responses throughout the process of neurosurgery and provides an early biomarker for surgical guidance. However, intraoperative CBF imaging has been challenging due to animal's motion and position change during the surgery. In this paper, we presented a design of an operation bench integrated with laser speckle contrast imager which enables monitoring of the CBF intraoperatively. With a specially designed stereotaxic frame and imager, we were able to monitor the CBF changes in both hemispheres during the rodent surgery. The rotatable design of the operation plate and implementation of online image registration allow the technician to move the animal without disturbing the CBF imaging during surgery. The performance of the system was tested by middle cerebral artery occlusion model of rats.
authors.affiliation: Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, China.
authors.forename: Hangdao
authors.initials: H
authors.lastname: Li
authors.affiliation: Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yuan
authors.affiliation: School of Media and Design, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Caihong
authors.initials: C
authors.lastname: Wu
authors.affiliation: Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, China.
authors.forename: Hongyang
authors.initials: H
authors.lastname: Lu
authors.affiliation: Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, China.
authors.forename: Shanbao
authors.initials: S
authors.lastname: Tong
mesh_terms: D000818:Animals; D002560:Cerebrovascular Circulation; D004867:Equipment Design; D020244:Infarction, Middle Cerebral Artery / Q000503:physiopathology / Q000601:surgery; D008297:Male; D057054:Molecular Imaging / Q000295:instrumentation*; D016343:Monitoring, Intraoperative / Q000295:instrumentation*; D051381:Rats