title: [Intensity loss of two-photon excitation fluorescence microscopy images of mouse oocyte chromosomes].
abstract: As an optical microscope with high resolution, two-photon excitation (TPE) fluorescence microscope is widely used in noninvasive 3D optical imaging of biological samples. Compared with confocal laser scanning microscope, TPE fluorescence microscope provides a deeper detecting depth. In spite of that, the image quality of sample always declines as the detecting depth increases when a noninvasive 3D optical imaging of thicker samples is performed. Mouse oocytes with a large diameter, which play an important role in clinical and biological fields, have obvious absorption and scattering effects. In the present paper, we performed compensation for two-photon fluorescence images of mouse oocyte chromosomes. Using volume as a parameter, the attenuation degree of these chromosomes was also studied. The result of our data suggested that there exists a severe axial intensity loss in two-photon microscopic images of mouse oocytes due to the absorption and scattering effects. It is necessary to make compensation for these images of mouse oocyte chromosomes obtained from two-photon microscopic system. It will be specially needed in studying the quantitative three-dimensional information of mouse oocytes.
authors.forename: Feng-Ying
authors.initials: FY
authors.lastname: Zhao
authors.forename: Hong-Xin
authors.initials: HX
authors.lastname: Wu
authors.forename: Die-Yan
authors.initials: DY
authors.lastname: Chen
authors.forename: Wan-Yun
authors.initials: WY
authors.lastname: Ma
mesh_terms: D000818:Animals; D002875:Chromosomes; D005453:Fluorescence; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009865:Oocytes; D017785:Photons