title: Role of feed forward neural networks coupled with genetic algorithm in capitalizing of intracellular alpha-galactosidase production by Acinetobacter sp.
abstract: Alpha-galactosidase production in submerged fermentation by Acinetobacter sp. was optimized using feed forward neural networks and genetic algorithm (FFNN-GA). Six different parameters, pH, temperature, agitation speed, carbon source (raffinose), nitrogen source (tryptone), and K2HPO4, were chosen and used to construct 6-10-1 topology of feed forward neural network to study interactions between fermentation parameters and enzyme yield. The predicted values were further optimized by genetic algorithm (GA). The predictability of neural networks was further analysed by using mean squared error (MSE), root mean squared error (RMSE), mean absolute error (MAE), mean absolute percentage error (MAPE), and R2-value for training and testing data. Using hybrid neural networks and genetic algorithm, alpha-galactosidase production was improved from 7.5 U/mL to 10.2 U/mL.
authors.affiliation: Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Andhra Pradesh 500 085, India.
authors.forename: Sirisha
authors.initials: S
authors.lastname: Edupuganti
authors.affiliation: Department of Chemical Engineering, Monash University, Clayton, VIC 3800, Australia.
authors.forename: Ravichandra
authors.identifier: 0000-0003-4092-0170
authors.initials: R
authors.lastname: Potumarthi
authors.affiliation: Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, Andhra Pradesh 500 607, India.
authors.forename: Thadikamala
authors.initials: T
authors.lastname: Sathish
authors.affiliation: Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Andhra Pradesh 500 085, India.
authors.forename: Lakshmi Narasu
authors.identifier: 0000-0001-5995-099X
authors.initials: LN
authors.lastname: Mangamoori
mesh_terms: D000150:Acinetobacter / Q000254:growth & development / Q000378:metabolism*; D000465:Algorithms; D002244:Carbon / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005285:Fermentation; D016571:Neural Networks, Computer; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism; D013696:Temperature; D000519:alpha-Galactosidase / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D002244:Carbon; D000519:alpha-Galactosidase; D009584:Nitrogen