title: The interaction of bee products with temozolomide in human diffuse astrocytoma, glioblastoma multiforme and astroglia cell lines.
abstract: In the present study, we investigated the influence of extracts from Salix spp. honey (ESH), beebread (EBB), and royal jelly (ERJ) with and without temozolomide (TMZ) on cell lines derived from a patient with diffuse astrocytoma (DASC), human glioblastoma multiforme (U87MG), and normal human astroglia (SVGp12). DASC was identified by immunocytochemistry. TMZ (20 μM) in combination with ESH (30 μg/mL), EBB (50 μg/mL), and ERJ (30 μg/mL) has stronger cytotoxic activity on U87MG cells after 72 h (20.0, 26.5, and 29.3% of control, respectively) than TMZ alone (about 6% of control). An increase of the cytotoxic effect and inhibition of DNA synthesis in SVGp12 were detected after administering TMZ with the studied extracts. NF-κB p50 subunit was reduced in U87MG cells after treatment with ESH (70.9%) and ESH + TMZ (74.7%). A significant decline of MMP-9 and MMP-2 secretion in cultured U87MG was detected after incubation with EBB (42.9% and 73.0%, respectively) and EBB + TMZ (38.4% and 68.5%, respectively). In conclusion, the use of bee products may increase the cytotoxic effect of TMZ in U87MG but also in SVGp12 cell line. It is important to note that the U87MG cells were sensitive to natural bee products, although there was no influence of natural bee products on the DASC cells.
authors.affiliation: a Department of Bromatology , Medical University of Bialystok , Bialystok , Poland.
authors.forename: Maria H
authors.initials: MH
authors.lastname: Borawska
authors.forename: Renata
authors.initials: R
authors.lastname: Markiewicz-Żukowska
authors.forename: Sylwia K
authors.initials: SK
authors.lastname: Naliwajko
authors.forename: Justyna
authors.initials: J
authors.lastname: Moskwa
authors.forename: Emilia
authors.initials: E
authors.lastname: Bartosiuk
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Socha
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Surażyński
authors.forename: Jan
authors.initials: J
authors.lastname: Kochanowicz
authors.forename: Zenon
authors.initials: Z
authors.lastname: Mariak
mesh_terms: D000328:Adult; D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D001254:Astrocytoma / Q000473:pathology*; D001516:Bees; D045744:Cell Line, Tumor; D003606:Dacarbazine / Q000031:analogs & derivatives* / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology*; D005909:Glioblastoma / Q000473:pathology*; D006722:Honey / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D011429:Propolis / Q000494:pharmacology*; D013048:Specimen Handling; D000077204:Temozolomide; D000099219:Royal Jelly
chemical_list: D003606:Dacarbazine; D005227:Fatty Acids; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D016328:NF-kappa B; D011429:Propolis; D000077204:Temozolomide; D000099219:Royal Jelly; C522361:MMP2 protein, human; C579270:MMP9 protein, human