title: Preparation and evaluation of silica-UIO-66 composite as liquid chromatographic stationary phase for fast and efficient separation.
abstract: A silica-UIO-66 composite was fabricated by a simple hydrothermal method and then applied as liquid chromatographic stationary phase for fast and efficient separation. X-ray diffraction patterns showed the presence of UIO-66 crystals in the silica-UIO-66 composites; while scanning electron microscope (SEM) images revealed that silica-UIO-66 composites were a homogeneous mixture of silica bead and UIO-66 crystals. A variety of substituted aromatics, chlorobenzene compounds and polycyclic aromatic hydrocarbons (PAHs) were used to evaluate the retention properties of the silica-UIO-66 composite packed column. Under the optimized conditions, baseline separation of ethylbenzene (EB) and styrene was obtained with high resolution and short retention time. In addition, the silica-UIO-66 composite packed column also showed some advantages in separation of positional isomers, with which baseline separation of EB and xylene, chlorotoluene and dichlorobenzene isomers was achieved. Moreover, the retention mechanisms of these compounds were also discussed in detail. The relative standard deviations (RSDs) for the separation of EB and xylene, chlorotoluene and dichlorobenzene isomers, as well as EB and styrene were 0.42-0.9%, 1.0-1.9%, 0.75-2.0%, and 0.9-2.1% for the retention time, peak area, peak height, and half peak width, respectively. The column efficiencies for EB, p-chlorotoluene, p-dichlorobenzene and styrene were 8780, 9060, 9990 and 5130 plates/m. The successful applications suggested high potentials of silica-MOFs composite as stationary phase for fast and efficient liquid chromatography separation.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China.
authors.forename: Jiangnan
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China; Longyan Entry-Exit Inspection and Quarantine Bureau of P.R.C, Longyan≤/org≥, Fujian, 364000, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Chen
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China; Testing Center, The Sport Science Research Center, Fuzhou University, Fuzhou, Fujian, 350002, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Tong
authors.affiliation: Testing Center, The Sport Science Research Center, Fuzhou University, Fuzhou, Fujian, 350002, China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Lu
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China. Electronic address: zianlin@fzu.edu.cn.
authors.forename: Zian
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China; Testing Center, The Sport Science Research Center, Fuzhou University, Fuzhou, Fujian, 350002, China. Electronic address: zlan@fzu.edu.cn.
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
mesh_terms: D001555:Benzene Derivatives / Q000737:chemistry; D002722:Chlorobenzenes / Q000737:chemistry; D002853:Chromatography, Liquid / Q000295:instrumentation*; D007536:Isomerism; D008855:Microscopy, Electron, Scanning; D012822:Silicon Dioxide / Q000737:chemistry*; D020058:Styrene / Q000737:chemistry; D014050:Toluene / Q000031:analogs & derivatives / Q000737:chemistry; D014961:X-Ray Diffraction; D014992:Xylenes / Q000737:chemistry
chemical_list: D001555:Benzene Derivatives; D002722:Chlorobenzenes; D014992:Xylenes; D014050:Toluene; D020058:Styrene; D012822:Silicon Dioxide; C018511:4-dichlorobenzene; C031294:chlorobenzene; C004912:ethylbenzene; C029049:4-chlorotoluene
keywords: Fast separation; High performance chromatography (HPLC); Metal-organic frameworks (MOFs); Positional isomers; Silica-UIO-66 composite