title: Ophiobolin A from Bipolaris oryzae perturbs motility and membrane integrities of porcine sperm and induces cell death on mammalian somatic cell lines.
abstract: Bipolaris oryzae is a phytopathogenic fungus causing a brown spot disease in rice, and produces substance that strongly perturbs motility and membrane integrities of boar spermatozoa. The substance was isolated from the liquid culture of the fungal strain using extraction and a multi-step semi-preparative HPLC procedures. Based on the results of mass spectrometric and 2D NMR techniques, the bioactive molecule was identified as ophiobolin A, a previously described sesterterpene-type compound. The purified ophiobolin A exhibited strong motility inhibition and viability reduction on boar spermatozoa. Furthermore, it damaged the sperm mitochondria significantly at sublethal concentration by the dissipation of transmembrane potential in the mitochondrial inner membrane, while the plasma membrane permeability barrier remained intact. The study demonstrated that the cytotoxicity of ophiobolin A toward somatic cell lines is higher by 1-2 orders of magnitude compared to other mitochondriotoxic mycotoxins, and towards sperm cells unique by replacing the progressive motility by shivering tail beating at low exposure concentration.
authors.affiliation: Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged H-6726, Hungary. bencsikotto@gmail.com.
authors.forename: Ottó
authors.initials: O
authors.lastname: Bencsik
authors.affiliation: Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged H-6726, Hungary. pappt@bio.u-szeged.hu.
authors.forename: Tamás
authors.initials: T
authors.lastname: Papp
authors.affiliation: Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged H-6726, Hungary. bertamate91@gmail.com.
authors.forename: Máté
authors.initials: M
authors.lastname: Berta
authors.affiliation: Institute of Pharmaceutical Analysis, University of Szeged, Somogyi u. 4, Szeged H-6720, Hungary. zanaa@pharm.u-szeged.hu.
authors.forename: Annamária
authors.initials: A
authors.lastname: Zana
authors.affiliation: Institute of Pharmaceutical Analysis, University of Szeged, Somogyi u. 4, Szeged H-6720, Hungary. forgop@ektf.hu.
authors.forename: Péter
authors.initials: P
authors.lastname: Forgó
authors.affiliation: Institute of Pharmaceutical Analysis, University of Szeged, Somogyi u. 4, Szeged H-6720, Hungary. g.dombi@pharm.u-szeged.hu.
authors.forename: György
authors.initials: G
authors.lastname: Dombi
authors.affiliation: Department of Food and Environmental Sciences, Viikinkaari 9, University of Helsinki, Agnes Sjöbergin katu 2, Helsinki FI-00014, Finland. mariande@mappi.helsinki.fi.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Andersson
authors.affiliation: Department of Food and Environmental Sciences, Viikinkaari 9, University of Helsinki, Agnes Sjöbergin katu 2, Helsinki FI-00014, Finland. mirja.salkinoja-salonen@helsinki.fi.
authors.forename: Mirja
authors.initials: M
authors.lastname: Salkinoja-Salonen
authors.affiliation: Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged H-6726, Hungary. mucor1959@gmail.com.
authors.forename: Csaba
authors.initials: C
authors.lastname: Vágvölgyi
authors.affiliation: Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged H-6726, Hungary. andras.j.szekeres@gmail.com.
authors.forename: András
authors.initials: A
authors.lastname: Szekeres
mesh_terms: D000818:Animals; D001203:Ascomycota; D002415:Cats; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability / Q000187:drug effects; D008297:Male; D051379:Mice; D054830:Sesterterpenes / Q000633:toxicity*; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D013552:Swine
chemical_list: D054830:Sesterterpenes; C040764:ophiobolin A