title: Effects of deer bone extract on the expression of pro-inflammatory cytokine and cartilage-related genes in monosodium iodoacetate-induced osteoarthritic rats.
abstract: Deer bone extract has the potential to relieve the discomfort or the articular cartilaginous damage associated with osteoarthritic (OA) and may be useful as a natural supplement for OA treatment without serious side effects. We analyzed the expression of pro-inflammatory cytokine and cartilage-related genes in monosodium iodoacetate-induced OA rats. Increases in the levels of serum pro-inflammatory cytokines, such as interleukin-1β, interleukin-6, and tumor necrosis factor-α were significantly inhibited by the administration of deer bone extract (p<0.05). Decreases in the expression of collagen type II (COL2) and tissue inhibitors of metalloproteinases (TIMPs) mRNAs in the cartilage were significantly inhibited by deer bone extract treatment (p<0.05). The deer bone extract significantly suppressed the expression of matrix metalloproteinases (MMPs) mRNAs in the cartilage. The deer bone extract induced the up-regulation of COL2 and TIMP mRNAs and the down-regulation of MMP mRNAs by suppressing the expression of pro-inflammatory cytokine mRNAs.
authors.affiliation: a Department of Food and Nutrition , Korea University , Seoul , Korea.
authors.forename: Hyunji
authors.initials: H
authors.lastname: Lee
authors.forename: Hyeon-Son
authors.initials: HS
authors.lastname: Choi
authors.forename: Yooheon
authors.initials: Y
authors.lastname: Park
authors.forename: Chang Won
authors.initials: CW
authors.lastname: Ahn
authors.forename: Sung Ug
authors.initials: SU
authors.lastname: Jung
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Hyung Joo
authors.initials: HJ
authors.lastname: Suh
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001842:Bone and Bones / Q000737:chemistry*; D002358:Cartilage, Articular / Q000187:drug effects / Q000378:metabolism*; D016207:Cytokines / Q000097:blood / Q000378:metabolism*; D003670:Deer; D004435:Eating / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D019807:Iodoacetic Acid / Q000009:adverse effects*; D008297:Male; D010003:Osteoarthritis / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D019807:Iodoacetic Acid
keywords: deer bone extract; matrix metalloproteinase; osteoarthritis; pro-inflammatory cytokines; tissue inhibitors of metalloproteinases