title: Effect of IL-17A on the migration and invasion of NPC cells and related mechanisms.
abstract: In carcinogenesis, inflammasomes may play contradictory roles through facilitating anti-tumor immunity or inducing oncogenic factors. Their function in cancer remains poorly characterized. In this study, we explored the effect of interleukin-17A (IL-17A) on the migration and invasion activity of nasopharyngeal carcinoma (NPC) cell lines and account for related mechanisms. Our results revealed that exogenous IL-17A promoted cell migration and invasion significantly in both NPC-039 and CNE-2Z cell lines. In addition, the expression of matrix metalloproteinase-2 (MMP-2)/-9 and Vimentin could be elevated by IL-17A stimulation; meanwhile the expression of E-cadherin was decreased. The results also show that IL-17A could activate the p38 signaling pathway in IL-17A-stimulated NPC-039 and CNE-2Z cell lines. Combining treatment with a p38 inhibitor (SB203580) resulted in decreased invasion capabilities of NPC-039 and CNE-2Z cell lines. SB203580 also inhibited the expression of MMP-2/-9 and increased the expression of E-cadherin in IL-17A-stimulated NPC-039 and CNE-2Z cell lines. IL-17A also could activate NF-κB in NPC-039 and CNE-2Z cell lines. In summary, our data show that IL-17A promote the cell migration and invasion of NPC cells. The effect of IL-17A on cell migration and invasion may be mediated via regulation of the expression of MMP-2/-9 and epithelial-mesenchymal transition (EMT) via p38-NF-κB signaling pathway. Thus, IL-17A or its related signaling pathways may be a promising target for preventing and inhibiting NPC metastasis.
authors.affiliation: Department of Otolaryngology head and neck surgery, Second Hospital of Xi'an Jiaotong University, Xi'an, China; Department of Otolaryngology head and neck surgery, Affiliated People's Hospital of Hubei Medical University, Shiyan, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology head and neck surgery, Hospital Affiliated to Ningxia Medical University, Yin chuan, China.
authors.forename: Ruixia
authors.initials: R
authors.lastname: Ma
authors.affiliation: Department of Otolaryngology head and neck surgery, Hospital Affiliated to Ningxia Medical University, Yin chuan, China.
authors.forename: Zhaopeng
authors.initials: Z
authors.lastname: Kang
authors.affiliation: Department of Otolaryngology head and neck surgery, Second Hospital of Xi'an Jiaotong University, Xi'an, China.
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology head and neck surgery, Affiliated People's Hospital of Hubei Medical University, Shiyan, China.
authors.forename: Hongcheng
authors.initials: H
authors.lastname: Ding
authors.affiliation: Department of Otolaryngology head and neck surgery, Affiliated People's Hospital of Hubei Medical University, Shiyan, China.
authors.forename: Weina
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Obstetrics and Gynecology, the Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an, P. R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Department of Otolaryngology head and neck surgery, Second Hospital of Xi'an Jiaotong University, Xi'an, China.
authors.forename: Min
authors.initials: M
authors.lastname: Xu
mesh_terms: D015820:Cadherins / Q000378:metabolism; D002277:Carcinoma; D045744:Cell Line, Tumor; D006801:Humans; D020381:Interleukin-17 / Q000494:pharmacology*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D009361:Neoplasm Invasiveness / Q000517:prevention & control*; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D015854:Up-Regulation / Q000187:drug effects; D014746:Vimentin / Q000378:metabolism
chemical_list: D015820:Cadherins; D020381:Interleukin-17; D014746:Vimentin; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9