title: Application of desorption electrospray ionization mass spectrometry imaging in breast cancer margin analysis.
abstract: Distinguishing tumor from normal glandular breast tissue is an important step in breast-conserving surgery. Because this distinction can be challenging in the operative setting, up to 40% of patients require an additional operation when traditional approaches are used. Here, we present a proof-of-concept study to determine the feasibility of using desorption electrospray ionization mass spectrometry imaging (DESI-MSI) for identifying and differentiating tumor from normal breast tissue. We show that tumor margins can be identified using the spatial distributions and varying intensities of different lipids. Several fatty acids, including oleic acid, were more abundant in the cancerous tissue than in normal tissues. The cancer margins delineated by the molecular images from DESI-MSI were consistent with those margins obtained from histological staining. Our findings prove the feasibility of classifying cancerous and normal breast tissues using ambient ionization MSI. The results suggest that an MS-based method could be developed for the rapid intraoperative detection of residual cancer tissue during breast-conserving surgery.
authors.affiliation: Departments of Neurosurgery.
authors.forename: David
authors.initials: D
authors.lastname: Calligaris
authors.affiliation: Departments of Neurosurgery, Radiology, Surgery, and.
authors.forename: Diana
authors.initials: D
authors.lastname: Caragacianu
authors.affiliation: Departments of Neurosurgery.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Liu
authors.affiliation: Departments of Neurosurgery.
authors.forename: Isaiah
authors.initials: I
authors.lastname: Norton
authors.affiliation: Bruker Daltonics, Billerica, MA 01821; and.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Thompson
authors.affiliation: Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02215.
authors.forename: Andrea L
authors.initials: AL
authors.lastname: Richardson
authors.affiliation: Surgery, and.
authors.forename: Mehra
authors.initials: M
authors.lastname: Golshan
authors.affiliation: Bruker Daltonics, Billerica, MA 01821; and.
authors.forename: Michael L
authors.initials: ML
authors.lastname: Easterling
authors.affiliation: Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02215.
authors.forename: Sandro
authors.initials: S
authors.lastname: Santagata
authors.affiliation: Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115;
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Dillon
authors.affiliation: Radiology.
authors.forename: Ferenc A
authors.initials: FA
authors.lastname: Jolesz
authors.affiliation: Departments of Neurosurgery, Radiology, Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02215 nathalie_agar@dfci.harvard.edu.
authors.forename: Nathalie Y R
authors.initials: NY
authors.lastname: Agar
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D018567:Breast Neoplasms, Male / Q000378:metabolism / Q000473:pathology*; D005227:Fatty Acids / Q000737:chemistry; D005260:Female; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008297:Male; D008408:Mastectomy; D008875:Middle Aged; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology*; D019301:Oleic Acid / Q000737:chemistry; D012008:Recurrence; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D014408:Biomarkers, Tumor; D005227:Fatty Acids; D008055:Lipids; D019301:Oleic Acid
keywords: FT-ICR MS; intrasurgical diagnosis; metabolites; molecular pathology