title: Vertically scanned laser sheet microscopy.
abstract: Laser sheet microscopy is a widely used imaging technique for imaging the three-dimensional distribution of a fluorescence signal in fixed tissue or small organisms. In laser sheet microscopy, the stripe artifacts caused by high absorption or high scattering structures are very common, greatly affecting image quality. To solve this problem, we report here a two-step procedure which consists of continuously acquiring laser sheet images while vertically displacing the sample, and then using the variational stationary noise remover (VSNR) method to further reduce the remaining stripes. Images from a cleared murine colon acquired with a vertical scan are compared with common stitching procedures demonstrating that vertically scanned light sheet microscopy greatly improves the performance of current light sheet microscopy approaches without the need for complex changes to the imaging setup and allows imaging of elongated samples, extending the field of view in the vertical direction.
authors.affiliation: Chinese Academy of Sciences, Key Laboratory of Molecular Imaging, Beijing 100190, China.
authors.forename: Di
authors.initials: D
authors.lastname: Dong
authors.affiliation: Swiss Federal Institute of Technology (ETH-Zurich), Institute for Biomedical Engineering, Switzerland.
authors.forename: Alicia
authors.initials: A
authors.lastname: Arranz
authors.affiliation: Xidian University, Xian, School of Life Science and Technology, Shaanxi 710071, China.
authors.forename: Shouping
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Chinese Academy of Sciences, Key Laboratory of Molecular Imaging, Beijing 100190, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Chinese Academy of Sciences, Key Laboratory of Molecular Imaging, Beijing 100190, China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Shi
authors.affiliation: Harbin University of Science and Technology, School of Automation, Harbin 150080, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Xidian University, Xian, School of Life Science and Technology, Shaanxi 710071, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Shen
authors.affiliation: Chinese Academy of Sciences, Key Laboratory of Molecular Imaging, Beijing 100190, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
authors.affiliation: Universidad Carlos III of Madrid, Department of Bioengineering and Aerospace Engineering, Madrid 28911, SpainfInstituto de Investigación Sanitaria del Hospital Gregorio Marañón, Experimental Medicine and Surgery Unit, Madrid 28007, SpaingFoundation for Re.
authors.forename: Jorge
authors.initials: J
authors.lastname: Ripoll
mesh_terms: D000465:Algorithms; D000818:Animals; D016477:Artifacts; D003106:Colon / Q000033:anatomy & histology; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*