title: Identification of putative fertility markers in seminal plasma of crossbred bulls through differential proteomics.
abstract: Sub-fertility is a major problem in crossbred bulls leading to disintegration of breeding systems and huge economic loss. Identification of some potential biomarkers to determine the latent fertility of bulls accurately has long been the interest of researchers. In this study, we analyzed the proteome of seminal plasma (SP) from bulls with varying fertility to identify the fertility-associated proteins. The proteomic profile of high- and low-fertile bulls was compared by two-dimensional difference gel electrophoresis and differentially expressed proteins were identified through matrix-assisted laser desorption/ionization-time of flight/mass spectrometry. Out of the 18 differentially expressed proteins (P < 0.05), 9 were overexpressed in SP of high-fertile bulls and 9 were overexpressed in SP of low-fertile bulls. The differential expressions ranged from 1.5- to 5.5-fold between the two groups, where protection of telomeres-1 protein (POT1) was highly overexpressed (2.9-fold) in high-fertile group and prostaglandin E2 receptor EP3 (PTGER3) was highly abundant (5.5-fold) in low-fertile group. The protein interaction network was elucidated using STRING software tool, and the functional bioinformatics analysis was done using Blast2Go software. Most of the differentially expressed proteins were found to be involved in cellular processes and biological regulation with binding and catalytic function. It is inferred that the expression of certain proteins in the SP varied with bull fertility, and concurrent appraisal of their expression along with other fertility assays may help in determining bull fertility.
authors.affiliation: Theriogenology Lab, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: M K Muhammad
authors.initials: MK
authors.lastname: Aslam
authors.affiliation: Theriogenology Lab, National Dairy Research Institute, Karnal, Haryana, India. Electronic address: ogkumaresan@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Kumaresan
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Vinay K
authors.initials: VK
authors.lastname: Sharma
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Md
authors.initials: M
authors.lastname: Tajmul
authors.affiliation: Theriogenology Lab, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: Shivani
authors.initials: S
authors.lastname: Chhillar
authors.affiliation: Artificial Breeding Research Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Chakravarty
authors.affiliation: Theriogenology Lab, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: A
authors.initials: A
authors.lastname: Manimaran
authors.affiliation: Artificial Breeding Research Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: T K
authors.initials: TK
authors.lastname: Mohanty
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: A
authors.initials: A
authors.lastname: Srinivasan
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India. Electronic address: savita11@gmail.com.
authors.forename: Savita
authors.initials: S
authors.lastname: Yadav
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002417:Cattle; D019295:Computational Biology; D005298:Fertility; D020869:Gene Expression Profiling; D006824:Hybridization, Genetic; D008297:Male; D040901:Proteomics; D012661:Semen / Q000378:metabolism*
chemical_list: D015415:Biomarkers
keywords: 2D-DIGE; Bioinformatics; Crossbred bulls; Seminal plasma