title: FACT plays a major role in histone dynamics affecting VSG expression site control in Trypanosoma brucei.
abstract: Chromatin remodelling is involved in the transcriptional regulation of the RNA polymerase I transcribed variant surface glycoprotein (VSG) expression sites (ESs) of Trypanosoma brucei. We show that the T. brucei FACT complex contains the Pob3 and Spt16 subunits, and plays a key role in ES silencing. We see an inverse correlation between transcription and condensed chromatin, whereby FACT knockdown results in ES derepression and more open chromatin around silent ES promoters. Derepressed ESs show increased sensitivity to micrococcal nuclease (MNase) digestion, and a decrease in histones at silent ES promoters but not telomeres. In contrast, FACT knockdown results in more histones at the active ES, correlated with transcription shut-down. ES promoters are derepressed in cells stalled at the G2/M cell cycle stage after knockdown of FACT, but not in G2/M cells stalled after knockdown of cyclin 6. This argues that the observed ES derepression is a direct consequence of histone chaperone activity by FACT at the G2/M cell cycle stage which could affect transcription elongation, rather than an indirect consequence of a cell cycle checkpoint. These experiments highlight the role of the FACT complex in cell cycle-specific chromatin remodelling within VSG ESs.
authors.affiliation: Division of Cell and Molecular Biology, Sir Alexander Fleming Building, Imperial College London, South Kensington, London, SW7 2AZ, UK.
authors.forename: Viola
authors.initials: V
authors.lastname: Denninger
authors.forename: Gloria
authors.initials: G
authors.lastname: Rudenko
mesh_terms: D002453:Cell Cycle; D002843:Chromatin / Q000378:metabolism; D005786:Gene Expression Regulation; D006657:Histones / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000378:metabolism / Q000502:physiology*; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000096:biosynthesis*
chemical_list: D002843:Chromatin; D006657:Histones; D014157:Transcription Factors; D014643:Variant Surface Glycoproteins, Trypanosoma