title: Effect of polyunsaturated fatty acids on secretory phospholipase A2 type IIa in ectopic endometrial cells.
abstract: BACKGROUND
  Endometriosis is a common chronic inflammation which leads to infertility and chronic pelvic pain in affected women. Secretory phospholipase A2 type IIa (sPLA2IIa) is an acute phase reactant that is markedly increased in inflammatory disorders.
  
  
  OBJECTIVE
  To assess the effects of ω-3 and ω-6 polyunsaturated fatty acids (PUFAs) administration in endometrial cells culture on sPLA2IIa level and cell survival comparing homolog ectopic versus eutopic endometrial cells from endometriosis patients.
  
  
  METHODS
  In this experimental study, ectopic and eutopic endometrial tissue samples obtained from 15 endometriosis patients were immediately frozen. After thawing and tissue digestion, mixed stromal and endometrial gland cells were cultured for 8 days in three different culture media; balanced ω-3/ω-6, high ω-3 and high ω-6 PUFAs ratio. Cell survival was measured using 2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-(phenylamino) carbonyl-2H- tetrazolium hydroxide (XTT) method and sPLA2IIa level assessed with ELISA technique.
  
  
  RESULTS
  The sPLA2IIa level was significantly higher in the ectopic endometrial cell culture compared to the eutopic group for each of the three matched treatments (balanced, high ω-3 and high ω-6). Also the sPLA2IIa level in the ectopic endometrial cell group was remarkably increased by each of the three PUFAs treatments compared to control condition (p<0.05, p<0.01, p<0.05 respectively). Cell survival in the eutopic group was significantly decreased by high ω-6 culturing compared to control medium (p<0.05).
  
  
  CONCLUSIONS
  The increase in sPLA2IIa level in ectopic endometrial cells by fatty acid treatments (especially high ω-3), strengthens the hypothesis that PUFAs stimulate secretion of cytokines leading to increased sPLA2IIa level.
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. ; Nano Technology Research Center , Tabriz University of Medical Sciences, Tabriz , Iran .
authors.forename: Korosh
authors.initials: K
authors.lastname: Khanaki
authors.affiliation: Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran- Iran .
authors.forename: Ali
authors.initials: A
authors.lastname: Motavalizadeh Ardekani
authors.affiliation: Women's Reproductive Health Research Center , Alzahra Hospital , Tabriz University of Medical Sciences, Tabriz , Iran .
authors.forename: Alieh
authors.initials: A
authors.lastname: Ghassemzadeh
authors.affiliation: Women's Reproductive Health Research Center , Alzahra Hospital , Tabriz University of Medical Sciences, Tabriz , Iran .
authors.forename: Vahideh
authors.initials: V
authors.lastname: Shahnazi
authors.affiliation: Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran- Iran .
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Sadeghi
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Darabi
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Mehdizadeh
authors.affiliation: Sarem Cell Research Center (SCRC), Sarem Women's Hospital, Tehran , Iran .
authors.forename: Abotaleb
authors.initials: A
authors.lastname: Saremi
authors.affiliation: Women's Reproductive Health Research Center , Alzahra Hospital , Tabriz University of Medical Sciences, Tabriz , Iran .
authors.forename: Jafar
authors.initials: J
authors.lastname: Soleimani-Rad
authors.affiliation: Department of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran , Iran .
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Imani
authors.affiliation: Women's Reproductive Health Research Center , Alzahra Hospital , Tabriz University of Medical Sciences, Tabriz , Iran .
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nouri
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. ; Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran , Iran .
authors.forename: Ali
authors.initials: A
authors.lastname: Rahimipour
keywords: Cell culture; Endometriosis; Fatty acids; Secretory phospholipase A2 type IIa