title: [Synthesis and identification of artificial antigens of paneoniflorin].
abstract: Oxidation method with sodium iodide was used to synthesize immunogenic antigen (PF-BSA) and coating antigen (PF-OVA) of paeoniflorin. UV spectroscopy showed that paeoniflorin was successfully conjugated with BSA and OVA. After immunized by PF-BSA, the mice can produce anti-paeoniflorin antibodies specifically. The ELISA test results showed the high titer (1:12 800) and specificity (IC50 = 0.791 mg x L(-1)) of the antiserum from mice injected with PF-BSA. Also, the antiserum showed low cross activities against nine traditional Chinese medicine (TCM) of small molecules. These artificial antigens were successfully synthesized and the anti-paeoniflorin antibody well prepared, which provides the experimental basis for the further study of ELISA and its kit.
authors.forename: Hui-Hua
authors.initials: HH
authors.lastname: Qu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Su
authors.forename: Na-Na
authors.initials: NN
authors.lastname: He
authors.forename: Ye
authors.initials: Y
authors.lastname: Sun
authors.forename: Hui
authors.initials: H
authors.lastname: Kong
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Qing-Guo
authors.initials: QG
authors.lastname: Wang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis; D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005960:Glucosides / Q000737:chemistry* / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D039821:Monoterpenes / Q000737:chemistry* / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology; D006027:Glycosides
chemical_list: D000906:Antibodies; D000941:Antigens; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; D012710:Serum Albumin, Bovine; C015423:peoniflorin; D006027:Glycosides