title: [The role of IL-22 on thymus recovery and its function in mice following allogeneic bone marrow transplantation].
abstract: OBJECTIVE
  To explore the role of IL-22 on the recovery and function of thymus from graft-versus host disease (GVHD) mice after allogeneic bone marrow transplantation (allo-BMT).
  
  
  METHODS
  GVHD model was established by using of recipient male BALB/c and donor male C57BL/6 mice(6-8 W) respectively. The mice were divided into normal group, GVHD with IL-22 group (BS+IL-22) and without IL-22 group (BS+PBS). Numbers of thymus cells were detected at different time points. The ratio of T cell subsets from thymus was observed by flow cytometry. Percentages of IFN-γ-producing and IL-17-producing CD4+ T or CD8+ T cells were detected.
  
  
  RESULTS
  The total number of thymus cells in BS+IL-22 mice [(14.6±5.1)×10⁴] was significantly higher than that in BS+PBS mice [(6.2±2.9)×10⁴] at 14 days after allo-BMT. Thymus cells in BS+IL-22 mice expanded continuously and reached at the level of normal mice, which were still higher than that in BS+PBS group. Although there was no impact on the ratio of mature CD4+ and CD8+ T cell from thymus, the percentage of immature CD4+CD8+ T cell increased obviously in mice treated with IL-22. Percentages of IFN-γ+CD4+ T cell [Th1:(2.42±0.75)%] and IFN-γ+CD8+ T cell [Tc1:(5.44±0.47)%] were up-regulated by IL-22 treatment, whereas no changes were detected in IL-17+CD4+ T cell (Th17) and IL-17+CD8+ T cell (Tc17).
  
  
  CONCLUSIONS
  IL-22 accelerates the progress of thymus recovery, and increases the IFN-γ-producing ability of thymus CD4+ and CD8+ T cells from GVHD mice.
authors.affiliation: Department of Hematology, the Affiliated Hospital of Xuzhou Medical College; Transplantation Immunology Laboratory of Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Department of Hematology, the Affiliated Hospital of Xuzhou Medical College; Transplantation Immunology Laboratory of Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Department of Hematology, the Affiliated Hospital of Xuzhou Medical College; Transplantation Immunology Laboratory of Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Yin
authors.affiliation: Department of Hematology, the Affiliated Hospital of Xuzhou Medical College; Transplantation Immunology Laboratory of Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Pan
authors.affiliation: Department of Hematology, the Affiliated Hospital of Xuzhou Medical College; Transplantation Immunology Laboratory of Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Kailin
authors.initials: K
authors.lastname: Xu
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D015496:CD4-Positive T-Lymphocytes; D018414:CD8-Positive T-Lymphocytes; D006086:Graft vs Host Disease; D020381:Interleukin-17; D007378:Interleukins / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016176:T-Lymphocyte Subsets; D013950:Thymus Gland / Q000276:immunology*; D014184:Transplantation, Homologous; D000097043:Interleukin-22
chemical_list: D020381:Interleukin-17; D007378:Interleukins; D000097043:Interleukin-22