title: Effect of ultrasonic treatment on structure and antitumor activity of mycelial polysaccharides from Cordyceps gunnii.
abstract: Taking mycelial polysaccharides from Cordyceps gunnii (C. gunnii) as the study subject, the effect of ultrasonic power, time and concentration of polysaccharides on antitumor activity of the polysaccharides was investigated. The ultrasonic processing condition of the polysaccharides was optimized by using orthogonal test design, and determined to be 400 W, 15 min and 1g/L. The change of structures of polysaccharides before and after ultrasonic treatment was also studied. Results show that ultrasonic treatment did not change the characteristic attribute of polysaccharides from C. gunnii. The composition of monosaccharide residues and the category of glycosidic bond have not been changed. But the molecular weight and intrinsic viscosity was reduced, and the alpha-helicity was enhanced after ultrasonic treatment. It was possible that ultrasonic treatment is an effective way for enhancing antitumor activity of polysaccharides.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China. Electronic address: zhyuanzhu@tust.edu.cn.
authors.forename: Zhen-Yuan
authors.initials: ZY
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Pang
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Li
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors.forename: Xiao-Ran
authors.initials: XR
authors.lastname: Ge
authors.affiliation: Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture, Shanghai Ocean University, 201306 Shanghai, PR China. Electronic address: ljchen@shou.edu.cn.
authors.forename: Li-Jing
authors.initials: LJ
authors.lastname: Chen
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors.forename: Xiao-Cui
authors.initials: XC
authors.lastname: Liu
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lv
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors.forename: Guo-Ling
authors.initials: GL
authors.lastname: Dong
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors.forename: An-Jun
authors.initials: AJ
authors.lastname: Liu
authors.affiliation: Université Pierre et Marie Curie-Paris 6, Institut Parisien de Chimie Moléculaire, CNRS UMR 8232, 4 place Jussieu, 75005 Paris, France.
authors.forename: Yongmin
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000528:radiation effects; D045744:Cell Line, Tumor; D032661:Cordyceps / Q000528:radiation effects; D006801:Humans; D015394:Molecular Structure; D025282:Mycelium / Q000145:classification* / Q000528:radiation effects; D011134:Polysaccharides / Q000737:chemistry* / Q000528:radiation effects; D013016:Sound
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides
keywords: C. gunnii; Mycelial polysaccharide; Structure; Ultrasonic, Antitumor activity