title: Self-adjuvanting therapeutic peptide-based vaccine induce CD8+ cytotoxic T lymphocyte responses in a murine human papillomavirus tumor model.
abstract: Vaccine candidates for the treatment of human papillomavirus (HPV)-associated cancers are aimed to activate T-cells and induce development of cytotoxic anti-tumor specific responses. Peptide epitopes derived from HPV-16 E7 oncogenic protein have been identified as promising antigens for vaccine development. However, peptide-based antigens alone elicit poor cytotoxic T lymphocyte (CTL) responses and need to be formulated with an adjuvant (immunostimulant) to achieve the desired immune responses. We have reported the ability of polyacrylate 4-arm star-polymer (S4) conjugated with HPV-16 E744-57 (8Qmin) epitope to reduce and eradicate TC-1 tumor in the mouse model. Herein, we have studied the mechanism of induction of immune responses by this polymer-peptide conjugate and found prompt uptake of conjugate by antigen presenting cells, stimulating stronger CD8(+) rather than CD4(+) or NK cell responses.
authors.forename: Tzu-Yu
authors.initials: TY
authors.lastname: Liu
authors.forename: Ashwini Kumar
authors.initials: AK
authors.lastname: Giddam
authors.forename: Waleed M
authors.initials: WM
authors.lastname: Hussein
authors.forename: Zhongfan
authors.initials: Z
authors.lastname: Jia
authors.forename: Nigel A J
authors.initials: NA
authors.lastname: McMillan
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Monteiro
authors.forename: Istvan
authors.initials: I
authors.lastname: Toth
authors.affiliation: School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Queensland 4072, Australia. m.skwarczynski@uq.edu.au.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Skwarczynski
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000737:chemistry; D000818:Animals; D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical; D004337:Drug Carriers; D000939:Epitopes; D005260:Female; D007114:Immunization; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000276:immunology / Q000821:virology; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D050725:Papillomavirus E7 Proteins / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology; D030361:Papillomavirus Infections / Q000188:drug therapy* / Q000276:immunology / Q000821:virology; D053918:Papillomavirus Vaccines / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects* / Q000276:immunology / Q000821:virology; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D000180:Acrylic Resins; D000276:Adjuvants, Immunologic; D019496:Cancer Vaccines; D004337:Drug Carriers; D000939:Epitopes; D050725:Papillomavirus E7 Proteins; D053918:Papillomavirus Vaccines; C059731:oncogene protein E7, Human papillomavirus type 16; C006903:carbopol 940