title: Effect of Pleurotus tuber-regium polysaccharides supplementation on the progression of diabetes complications in obese-diabetic rats.
abstract: In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties. Extracellular polysaccharides from three different strains of Pleurotus tuber-regium were extracted and labeled as HP (high-percentage), MP (medium-percentage) and LP (low-percentage). Obese- diabetes (OD) was induced by chronic high-fat diet plus streptozotocin (STZ) injections. Simultaneously to the diet, polysaccharides were orally administered to OD groups (20 mg/kg body weight/8-week), and categorized into OD+HP, OD+MP and OD+LP groups (n = 10/group), respectively. High-fat diet plus STZ-induced hyperglycemia was prominently attenuated by polysaccharides. Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats was attenuated, while, reduced MUFA/ PUFA and MUFA/SFA ratios were restored (P < 0.01) with polysaccharides treatment. Furthermore, elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations were controlled, and parallel restoration of decreased high-density lipoprotein (HDL) levels were found with polysaccharides supplementation. This hypolipidemic property might be associated with up-regulated liver PPAR-α mRNA expression and protein levels (P < 0.01). These findings concluded that stable fatty acid components and activated PPAR-α by polysaccharides may contribute to its hypoglycemic and hypolipidemic properties. Therefore, P. tuber-regium could be considered as nutritional supplement to treat diabetic complications.
authors.affiliation: Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih-Chien University, Taipei City 10462, Taiwan, Republic of China.
authors.forename: Hui-Yu
authors.initials: HY
authors.lastname: Huang
authors.forename: Mallikarjuna
authors.initials: M
authors.lastname: Korivi
authors.forename: Hui-Ting
authors.initials: HT
authors.lastname: Yang
authors.forename: Chi-Chang
authors.initials: CC
authors.lastname: Huang
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Chaing
authors.forename: Ying-Chieh
authors.initials: YC
authors.lastname: Tsai
mesh_terms: D000821:Animal Feed; D000818:Animals; D008078:Cholesterol, LDL / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000178:diet therapy*; D059305:Diet, High-Fat; D019587:Dietary Supplements; D018450:Disease Progression; D050171:Dyslipidemias / Q000150:complications / Q000178:diet therapy*; D004734:Energy Metabolism / Q000502:physiology; D005227:Fatty Acids / Q000097:blood; D005936:Glucans / Q000494:pharmacology; D006943:Hyperglycemia / Q000150:complications / Q000178:diet therapy*; D008297:Male; D009765:Obesity / Q000150:complications / Q000178:diet therapy; D053147:Plant Exudates / Q000494:pharmacology*; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000494:pharmacology*; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood
chemical_list: D008078:Cholesterol, LDL; D005227:Fatty Acids; D005936:Glucans; D053147:Plant Exudates; D011134:Polysaccharides; D014280:Triglycerides