title: Combining single-molecule manipulation and single-molecule detection.
abstract: Single molecule force manipulation combined with fluorescence techniques offers much promise in revealing mechanistic details of biomolecular machinery. Here, we review force-fluorescence microscopy, which combines the best features of manipulation and detection techniques. Three of the mainstay manipulation methods (optical traps, magnetic traps and atomic force microscopy) are discussed with respect to milestones in combination developments, in addition to highlight recent contributions to the field. An overview of additional strategies is discussed, including fluorescence based force sensors for force measurement in vivo. Armed with recent exciting demonstrations of this technology, the field of combined single-molecule manipulation and single-molecule detection is poised to provide unprecedented views of molecular machinery.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, United States.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Cordova
authors.affiliation: Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, United States.
authors.forename: Dibyendu Kumar
authors.initials: DK
authors.lastname: Das
authors.affiliation: Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, United States.
authors.forename: Harris W
authors.initials: HW
authors.lastname: Manning
authors.affiliation: Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, United States; Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN, United States. Electronic address: matt.lang@vanderbilt.edu.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Lang
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry; D018625:Microscopy, Atomic Force / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D057054:Molecular Imaging / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes