title: Purification and characterization of a novel antimicrobial peptide from sheep reproductive tract.
abstract: A novel antimicrobial peptide, SRTAP-40 has been purified and characterized from sheep reproductive tract. The isolation procedure entailed acetic acid extraction, gel filtration chromatography, and HPLC. SRTAP-40 is composed of 40 amino acid residues with a MW of 4,820.47 Da from MALDI-TOF-MS. Its N-terminal sequence was AYVLDEPKP. SRTAP-40 cDNA was cloned by 3'-RACE. SRTAP-40 showed activity against E. coli Staphylococcus aureus, Streptococcus sp. and, Candida albicans with MIC values of 12, 12, 24, 6 μg/ml, respectively. By BLAST search, SRTAP-40 had no significant similarity to any known peptide.
authors.affiliation: College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, 723000, Shaanxi, China, cchen2008@yahoo.com.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Chaofeng
authors.initials: C
authors.lastname: Ku
authors.forename: Xinwen
authors.initials: X
authors.lastname: Bo
authors.forename: Xinhua
authors.initials: X
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D001483:Base Sequence; D002176:Candida albicans / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D005836:Genitalia, Female / Q000737:chemistry*; D006461:Hemolysis / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D011817:Rabbits; D012756:Sheep
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides