title: [Preparation of a polyclonal antibody of Helicoverpa armigera arginine kinase in mice using DNA immunization with in vivo electroporation].
abstract: OBJECTIVE
  To prepare a polyclonal antibody against Helicoverpa armigera arginine kinase (HarmAK) in mice by DNA immunization with in vivo electroporation, and verify the specificity of the antibody by Western blotting.
  
  
  METHODS
  A recombinant expression plasmid pcDNA3-HarmAK was constructed and sequenced, and then was transferred into Kunming mice five times using electroporation. ELISA and Western blotting were used respectively to determine the titer of HarmAK antibody and detect the specificity of the monoclonal antibody.
  
  
  RESULTS
  ELISA showed that the titer of mouse anti-HarmAK serum reached 1:40 000 , and Western blotting demonstrated that this antiserum had a specific binding to both fusion protein His-HarmAK and AK protein of total protein of the Helicoverpa armigera.
  
  
  CONCLUSIONS
  We prepared a polyclonal antibody of HarmAK from the Helicoverpa armigera using DNA immunization with in vivo electroporation and the antibody has a high titer and specificity.
authors.affiliation: College of Life Science and Technology, Xinjiang University, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830046, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Life Science and Technology, Xinjiang University, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830046, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Zhao
authors.affiliation: College of Life Science and Technology, Xinjiang University, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830046, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: College of Life Science and Technology, Xinjiang University, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830046, China.
authors.forename: Fuchun
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Life Science and Technology, Xinjiang University, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830046, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Ma
authors.affiliation: College of Life Science and Technology, Xinjiang University, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830046, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Huang
authors.affiliation: College of Life Science and Technology, Xinjiang University, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830046, China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D001122:Arginine Kinase / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015153:Blotting, Western; D004247:DNA / Q000235:genetics / Q000276:immunology*; D018274:Electroporation; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization / Q000379:methods; D019476:Insect Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009036:Moths / Q000201:enzymology* / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D019476:Insect Proteins; D004247:DNA; D001122:Arginine Kinase