title: Efficacy of a combined nanoparticulate/calcium hydroxide root canal medication on elimination of Enterococcus faecalis.
abstract: The purpose of this study was to evaluate the efficacy of Ca(OH)2 with or without a silver nanoparticle suspension to eliminate Enterococcus faecalis from root canals. A total of 66 extracted human single-rooted teeth contaminated with E. faecalis were treated with 10% Ca(OH)2 alone, Ca(OH)2 with nanosilver or sterile water (as a negative control). Samples were obtained with paper points and Gates-Glidden burs at 1 and 7 days after root canal preparation and the number of colony-forming units (CFU) was determined. The number of CFUs observed after dressing with Ca(OH)2  + nanosilver was significantly less than the number observed with Ca(OH)2 alone after 1 or 7 days (P < 0.001, P < 0.001). No differences in antimicrobial properties were observed between the two time points in the Ca(OH)2  + nanosilver group (P > 0.05). Higher antimicrobial efficacy was observed in the Ca(OH)2 group after 7 days than 1 day (P < 0.001). This study highlighted the potential advantage of using a mixture of Ca(OH)2 and nanosilver for intracanal medicament.
authors.affiliation: Department of Endodontics, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Javidi
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Afkhami
authors.forename: Mina
authors.initials: M
authors.lastname: Zarei
authors.forename: Kiarash
authors.initials: K
authors.lastname: Ghazvini
authors.forename: Omid
authors.initials: O
authors.lastname: Rajabi
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D058491:Bacterial Load / Q000187:drug effects; D002126:Calcium Hydroxide / Q000627:therapeutic use*; D003786:Dental Pulp Cavity / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000187:drug effects*; D006801:Humans; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000627:therapeutic use*; D011897:Random Allocation; D012388:Root Canal Irrigants / Q000627:therapeutic use*; D018915:Root Canal Preparation / Q000295:instrumentation / Q000379:methods; D012834:Silver / Q000627:therapeutic use; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D012388:Root Canal Irrigants; D012834:Silver; D002126:Calcium Hydroxide
keywords: Enterococcus faecalis; calcium hydroxide; intracanal dressings; silver nanoparticles