title: Enhanced osteoinductivity of recombinant human bone morphogenetic protein-2 in combination with epidermal growth factor in a rabbit tibial defect model.
abstract: This study aims to explore the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on bone formation when treated with epidermal growth factor (EGF) using human mesenchymal stem cells (hMSCs) and a rabbit tibial defect model. The rhBMP-2 (250 ng/ml)+EGF (10 ng/ml) group showed higher alkaline phosphatase (ALP) activity, ALP expression, increased calcium amount than rhBMP-2 group. In micro-CT and histology results of animal experiments, the rhBMP-2+EGF group showed more amount of bone bridging compared to the rhBMP-2 group. Among the 8-week groups, the rhBMP-2+EGF group showed significantly higher percent bone volume and trabecular number compared to the rhBMP-2 group. The combined treatment with EGF and rhBMP-2 induced significantly higher bone formation compared to that of rhBMP-2 only in both hMSCs and a rabbit tibial defect model. Therefore, EGF is expected to facilitate bone formation effect of rhBMP-2 when both factors are treated in combination.
authors.affiliation: Department of Orthopedic Surgery, College of Medicine, SMG-SNU Boramae Medical Center, Seoul National University , Seoul , Korea .
authors.forename: Jae Hyup
authors.initials: JH
authors.lastname: Lee
authors.forename: Hae-Ri
authors.initials: HR
authors.lastname: Baek
authors.forename: Kyung Mee
authors.initials: KM
authors.lastname: Lee
authors.forename: Dong-Yeon
authors.initials: DY
authors.lastname: Lee
authors.forename: A-Young
authors.initials: AY
authors.lastname: Lee
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D001861:Bone Regeneration; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology; D013977:Tibia / Q000601:surgery*
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D011994:Recombinant Proteins; D004815:Epidermal Growth Factor
keywords: Bone morphogenetic protein-2; epidermal growth factor; osteogenesis; synergy