title: Synthesis, characterization, DFT calculation and biological activity of square-planar Ni(II) complexes with tridentate PNO ligands and monodentate pseudohalides. Part II.
abstract: Three square-planar complexes of Ni(II) with condensation derivative of 2-(diphenylphosphino)benzaldehyde and 4-phenylsemicarbazide and monodentate pseudohalides have been synthesized and characterized on the basis of the results of X-ray, NMR and IR spectroscopy and elemental analysis. Investigated complexes exhibited moderate antibacterial and cytotoxic activity. The most pronounced cytotoxic activity (in the range of cisplatin) to HeLa cell line was observed for ligand and all the complexes. Azido complex and ligand induced concentration dependent cell cycle arrest in the S phase, as well as decrease of percentage of cells in G1 phase, without significant increase of apoptotic fraction of cells. The interaction of the azido complex and ligand with CT-DNA results in changes in UV-Vis spectra typical for non-covalent bonding. The observed intrinsic binding constant of azido complex-CT-DNA and ligand-CT-DNA were 3.22 × 10(5) M(-1) and 2.79 × 10(5) M(-1). The results of DNA cleavage experiments showed that azido complex nicked supercoiled plasmid DNA.
authors.affiliation: Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
authors.forename: Milica
authors.initials: M
authors.lastname: Milenković
authors.affiliation: Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000 Ljubljana, Slovenia.
authors.forename: Andrej
authors.initials: A
authors.lastname: Pevec
authors.affiliation: Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000 Ljubljana, Slovenia.
authors.forename: Iztok
authors.initials: I
authors.lastname: Turel
authors.affiliation: Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoševa 12, P.O. Box 815, 11000 Belgrade, Serbia.
authors.forename: Miroslava
authors.initials: M
authors.lastname: Vujčić
authors.affiliation: Department of Microbiology and Immunology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, Serbia.
authors.forename: Marina
authors.initials: M
authors.lastname: Milenković
authors.affiliation: Institute for Oncology and Radiology of Serbia, Department of Experimental Oncology, Laboratory for Experimental Pharmacology, Pasterova 14, Belgrade, Serbia.
authors.forename: Katarina
authors.initials: K
authors.lastname: Jovanović
authors.affiliation: Institute for Oncology and Radiology of Serbia, Department of Experimental Oncology, Laboratory for Experimental Pharmacology, Pasterova 14, Belgrade, Serbia.
authors.forename: Nevenka
authors.initials: N
authors.lastname: Gligorijević
authors.affiliation: Institute for Oncology and Radiology of Serbia, Department of Experimental Oncology, Laboratory for Experimental Pharmacology, Pasterova 14, Belgrade, Serbia.
authors.forename: Siniša
authors.initials: S
authors.lastname: Radulović
authors.affiliation: Institució Catalana de Recerca i Estudis Avançats (ICREA), Pg. Lluís Companys 23, 08010 Barcelona, Spain; Institut de Química Computacional i Catàlisi and Departament de Química, Universitat de Girona, Campus Montilivi, 17071 Girona, Spain.
authors.forename: Marcel
authors.initials: M
authors.lastname: Swart
authors.affiliation: Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
authors.forename: Maja
authors.initials: M
authors.lastname: Gruden-Pavlović
authors.affiliation: Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
authors.forename: Kawther
authors.initials: K
authors.lastname: Adaila
authors.affiliation: Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
authors.forename: Božidar
authors.initials: B
authors.lastname: Cobeljić
authors.affiliation: Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia. Electronic address: kka@chem.bg.ac.rs.
authors.forename: Katarina
authors.initials: K
authors.lastname: Anđelković
mesh_terms: D000577:Amides / Q000737:chemistry; D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002417:Cattle; D002453:Cell Cycle / Q000187:drug effects; D060326:Chemistry Techniques, Synthetic; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D053837:DNA Cleavage / Q000187:drug effects; D004355:Drug Stability; D004583:Electrons; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D009532:Nickel / Q000737:chemistry*; D011789:Quantum Theory
chemical_list: D000577:Amides; D000970:Antineoplastic Agents; D056831:Coordination Complexes; D008024:Ligands; D009532:Nickel; D004247:DNA
keywords: Antimicrobial activity; Cytotoxicity; Nickel(II) complexes; Pseudohalides; X-ray crystal structures