title: Nhumirim virus, a novel flavivirus isolated from mosquitoes from the Pantanal, Brazil.
abstract: We describe the isolation of a novel flavivirus, isolated from a pool of mosquitoes identified as Culex (Culex) chidesteri collected in 2010 in the Pantanal region of west-central Brazil. The virus is herein designated Nhumirim virus (NHUV) after the name of the ranch from which the mosquito pool was collected. Flavivirus RNA was detected by real-time RT-PCR of homogenized mosquitoes and from the corresponding C6/36 culture supernatant. Based on full-genome sequencing, the virus isolate was genetically distinct from but most closely related to Barkedji virus (BJV), a newly described flavivirus from Senegal. Phylogenetic analysis demonstrated that NHUV grouped with mosquito-borne flaviviruses forming a clade with BJV. This clade may be genetically intermediate between the Culex-borne flaviviruses amplified by birds and the insect-only flaviviruses.
authors.affiliation: Laboratório de Flavivírus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Ministério da Saúde, Rio de Janeiro, RJ, Brazil, pauvolid@ioc.fiocruz.br.
authors.forename: Alex
authors.initials: A
authors.lastname: Pauvolid-Corrêa
authors.forename: Owen
authors.initials: O
authors.lastname: Solberg
authors.forename: Dinair
authors.initials: D
authors.lastname: Couto-Lima
authors.forename: Joan
authors.initials: J
authors.lastname: Kenney
authors.forename: Nicolau
authors.initials: N
authors.lastname: Serra-Freire
authors.forename: Aaron
authors.initials: A
authors.lastname: Brault
authors.forename: Rita
authors.initials: R
authors.lastname: Nogueira
authors.forename: Stanley
authors.initials: S
authors.lastname: Langevin
authors.forename: Nicholas
authors.initials: N
authors.lastname: Komar
mesh_terms: D000818:Animals; D001938:Brazil; D002522:Chlorocebus aethiops; D003465:Culex / Q000821:virology*; D005260:Female; D005416:Flavivirus / Q000145:classification* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013987:Ticks; D014709:Vero Cells
chemical_list: D012367:RNA, Viral