title: [Correlation analysis of bacterial biofilm formation and bacterial culture in chronic otitis media].
abstract: OBJECTIVE
  To study the correlation between the bacterial biofilm formation and bacterial culture in chronic otitis media.
  
  
  METHODS
  As a prospective reserch, we used scanning electron microscopy to examinate patients samples which collected from 32 cases of patients with chronic suppurative otitis media and middle ear cholesteatoma in the operations, and performed the middle ear secretions bacterial culture. According to the different types of chronic otitis media group, we analysised the relationship between chronic otitis media bacterial biofilm formation and the bacterial culture results.
  
  
  RESULTS
  Chronic suppurative otitis media (activity) and middle ear cholesteatoma bacterial biofilm formation rate were 87.5%, 81.3%, chi-square (P > 0.05). Compared bacterial biofilm results with the results of bacterial cultured in chronic otitis media, sensitivity was 70.37%, specificity was 60.00%, the misdiagnosis rate was 40.00%, the missed diagnosis was 29.63%, positive predictive value was 90. 46%, negative predictive value was 27.27%, accuracy was 68.75%. Youden index was 30. 37%, and Pearson correlation coefficient was 0.232 (P > 0.05).
  
  
  CONCLUSIONS
  Chronic suppurative otitis media (activity) and middle ear cholesteatoma bacteria had a higher biofilm formation rate. The routine bacterial culture results can't reflecte bacterial biofilm formation in chronic otitis media. We need to explore more reliable experimental methods to accurately reveal the infection status of chronic otitis media.
authors.forename: Xingzhi
authors.initials: X
authors.lastname: Gu
authors.forename: Abulajiang
authors.initials: A
authors.lastname: Tuoheti
authors.forename: Youledusi
authors.initials: Y
authors.lastname: Keyoumu
authors.forename: Xiuqing
authors.initials: X
authors.lastname: Cheng
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Tang
authors.forename: Dongmei
authors.initials: D
authors.lastname: Shi
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D001419:Bacteria / Q000254:growth & development; D018441:Biofilms; D018424:Cholesteatoma, Middle Ear / Q000382:microbiology*; D002908:Chronic Disease; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010035:Otitis Media, Suppurative / Q000382:microbiology*; D012680:Sensitivity and Specificity; D055815:Young Adult