title: Fast and automatic imaging of immunoenzyme-stained neuronal circuits in the whole brain of Drosophila.
abstract: Knowledge of neuronal wiring and morphogenesis in Drosophila is essential to understand brain function and dysfunction. The immunoenzyme method based on horseradish peroxidase/diaminobenzidine (HRP/DAB) provides high-contrast images to resolve details underlying neuronal architecture. However, the poor staining penetration and a lack of corresponding three-dimensional imaging methodology limit its application. Herein, we modified the HRP/DAB method to stain neuronal circuits in the whole brain of Drosophila. Furthermore, we found that imaging with the micro-optical sectioning tomography system provided a fast and automatic method that could dissect cell-specific neuroanatomical architecture at a submicron voxel resolution.
authors.affiliation: Huazhong University of Science and Technology-Wuhan National Laboratory for Optoelectronics, Britton Chance Center for Biomedical Photonics, Wuhan 430074, ChinabHuazhong University of Science and Technology, Department of Biomedical Engineering, MoE Key L.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Tian
authors.affiliation: Huazhong University of Science and Technology-Wuhan National Laboratory for Optoelectronics, Britton Chance Center for Biomedical Photonics, Wuhan 430074, ChinabHuazhong University of Science and Technology, Department of Biomedical Engineering, MoE Key L.
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Huazhong University of Science and Technology-Wuhan National Laboratory for Optoelectronics, Britton Chance Center for Biomedical Photonics, Wuhan 430074, ChinabHuazhong University of Science and Technology, Department of Biomedical Engineering, MoE Key L.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Huazhong University of Science and Technology-Wuhan National Laboratory for Optoelectronics, Britton Chance Center for Biomedical Photonics, Wuhan 430074, ChinabHuazhong University of Science and Technology, Department of Biomedical Engineering, MoE Key L.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Huazhong University of Science and Technology-Wuhan National Laboratory for Optoelectronics, Britton Chance Center for Biomedical Photonics, Wuhan 430074, ChinabHuazhong University of Science and Technology, Department of Biomedical Engineering, MoE Key L.
authors.forename: Hui
authors.initials: H
authors.lastname: Gong
authors.affiliation: Huazhong University of Science and Technology-Wuhan National Laboratory for Optoelectronics, Britton Chance Center for Biomedical Photonics, Wuhan 430074, ChinabHuazhong University of Science and Technology, Department of Biomedical Engineering, MoE Key L.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
mesh_terms: D015100:3,3'-Diaminobenzidine / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000166:cytology*; D001923:Brain Chemistry; D004330:Drosophila; D006651:Histocytochemistry / Q000379:methods; D006735:Horseradish Peroxidase / Q000378:metabolism; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007124:Immunoenzyme Techniques / Q000379:methods*; D041622:Tomography, Optical / Q000379:methods*
chemical_list: D015100:3,3'-Diaminobenzidine; D006735:Horseradish Peroxidase