title: Effect and mechanism of miRNA to osteosarcoma cell.
abstract: MicroRNA has proved to be low expression in many tumor cells. In addition, it was also proved that as a kind of cancer suppressor gene, miR-199a-3p in miRNA can affect the growth and invasion ability of tumor cells. This paper aims to discuss the effect of miRNA to osteosarcoma cell. It used synthetic mature miR-199a-3p sequence simulants to transfect osteosarcoma cell and took negative contrast sequence (NC mimics) transfection cell as negative contrast. After transfection, qRT-PCR was applied to detect the expression quantity of miR-199a-3p in every group. Western blot method was applied to detect the expression level of MCL-(1) protein and shear situation of PARP in groups of cells. Flow cytometry was used for detecting apoptosis rate of cells and the experimental result was made a statistical analysis. The result shows that in cells from experimental group of transfection miR-199a-3p sequence simulants, expression quantity of mi-R-199a-3p significantly increased while MCL⁻¹ protein expression decreased compared to control group. In addition, shear level of PARP protein and apoptosis rate of cells increased. The differences all had statistical significance (P<0.05). It was concluded that miR-199a-3p can effectively promote the apoptosis rate of osteosarcoma cells.
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital, Zhengzhou University,Zhengzhou, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Tian
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital, Zhengzhou University,Zhengzhou, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Wang
authors.affiliation: TheOtolaryngologicalDepartment, the First Affiliated Hospital, Zhengzhou University,Zhengzhou, China.
authors.forename: Ruiqing
authors.initials: R
authors.lastname: Di
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital, Zhengzhou University,Zhengzhou, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital, Zhengzhou University,Zhengzhou, China.
authors.forename: Guangheng
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
authors.forename: Zhifu
authors.initials: Z
authors.lastname: Li
mesh_terms: D017209:Apoptosis; D001859:Bone Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D006801:Humans; D035683:MicroRNAs / Q000032:analysis / Q000502:physiology*; D064549:Myeloid Cell Leukemia Sequence 1 Protein / Q000032:analysis; D012516:Osteosarcoma / Q000473:pathology*; D011065:Poly(ADP-ribose) Polymerases / Q000032:analysis
chemical_list: C579095:MCL1 protein, human; D035683:MicroRNAs; D064549:Myeloid Cell Leukemia Sequence 1 Protein; C531408:mirn199 microRNA, human; D011065:Poly(ADP-ribose) Polymerases