title: Self-assembly of cellulose nanoparticles as electrolyte additive for capillary electrophoresis separation.
abstract: In this work, a new cellulose derivative, octadecyl modified quaternized cellulose (ODMQC), was synthesized and used as additive in the background electrolyte for capillary electrophoresis. The derivative bearing hydrophobic groups and hydrophilic groups can self-assemble into a stable nano-scaled micelle structure in aqueous solution. When ODMQC was added in running buffer, the capillaries were shown to generate applicable anodal EOF over the investigated range of pH 3.0-12.0. Due to the lack of UV active groups, the ODMQC did not disturb the UV detection. It is shown that ODMQC-added capillaries allow the separation of basic proteins by reducing their adsorption onto the capillary wall. Also, the addition of ODMQC provides adequate separation of aromatic acids with low pKa values and improved separation of sulfa drugs. Moreover, it is demonstrated that the addition of ODMQC can incorporate an additional reversed-phase mechanism that improves the separation of neutral analytes.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Dihui
authors.initials: D
authors.lastname: Huang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Shanxia
authors.initials: S
authors.lastname: Jin
authors.affiliation: Hubei Key Laboratory of Oil Crops Lipid Chemistry and Nutrition at Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China.
authors.forename: Qianchun
authors.initials: Q
authors.lastname: Deng
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China. Electronic address: zbping@whu.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
mesh_terms: D002482:Cellulose / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D053758:Nanoparticles / Q000737:chemistry*; D011506:Proteins / Q000302:isolation & purification
chemical_list: D004573:Electrolytes; D011506:Proteins; D002482:Cellulose
keywords: Additive; Capillary electrophoresis; Cellulose derivative; Nanoparticle; Self assembly