title: Topiramate ameliorates abdominal aorta cross-clamping induced liver injury in rats.
abstract: OBJECTIVE
  Ischemia/reperfusion (I/R) injury in the liver occurs after a prolonged period of ischemia followed by restoration of hepatic blood perfusion. During the surgery of abdominal aorta, I/R injury causes damage to lower extremities and many organs, especially liver. The antioxidant and tumor necrosis factor-alpha (TNF-α) suppression effects of topiramate (TPM) have been reported in several studies. We evaluated the potential protective effect of TPM on cellular damage in liver tissue during I/R injury.
  
  
  METHODS
  Thirty male Wistar albino rats were divided into three groups: Control, I/R, and I/R plus TPM (I/R + TPM) groups. Laparotomy without I/R injury was performed in the control group. After laparotomy, cross-ligation of infrarenal abdominal aorta was applied for 2 h in I/R groups that was followed by 2 h of reperfusion. TPM (100 mg/kg/day) was orally administrated to the animals in the I/R + TPM group for seven consecutive days before I/R procedure.
  
  
  RESULTS
  The I/R group's TNF-α and interleukin-6 (IL-6) levels were significantly higher than those of the control (P = 0.010; P = 0.002) and I/R + TPM groups (P = 0.010; P = 0.002, respectively). Asymmetric dimethyl arginine (ADMA) levels of I/R group were higher than the control (P = 0.015) and I/R + TPM groups. I/R caused serious histopathological damage to liver tissue; however, TPM led to very low histopathological changes.
  
  
  CONCLUSIONS
  Our data demonstrated that TPM treatment prominently decreases the severity of liver I/R injury. TPM pretreatment may have preventive effects on liver injury via I/R during intra-abdominal surgery.
authors.affiliation: Department of Internal Medicine, Recep Tayyip Erdogan University, School of Medicine, Rize, Turkey.
authors.forename: Erkan
authors.initials: E
authors.lastname: Cure
authors.forename: Medine C
authors.initials: MC
authors.lastname: Cure
authors.forename: Levent
authors.initials: L
authors.lastname: Tumkaya
authors.forename: Yildiray
authors.initials: Y
authors.lastname: Kalkan
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Aydin
authors.forename: Aynur
authors.initials: A
authors.lastname: Kirbas
authors.forename: Arif
authors.initials: A
authors.lastname: Yilmaz
authors.forename: Suleyman
authors.initials: S
authors.lastname: Yuce
authors.forename: Mehmet F
authors.initials: MF
authors.lastname: Gokce
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D001120:Arginine / Q000031:analogs & derivatives / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005632:Fructose / Q000031:analogs & derivatives* / Q000494:pharmacology; D015850:Interleukin-6 / Q000097:blood; D008026:Ligation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy*; D000077236:Topiramate; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000927:Anticonvulsants; D015415:Biomarkers; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D000077236:Topiramate; D005632:Fructose; C018524:N,N-dimethylarginine; D001120:Arginine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase