title: The Study of "Dihydropyrimidinase Related Proteins (DRPs)" Expression Changes Influence in Malignant Astrocytoma Brain Tumor.
abstract: BACKGROUND
  Dihydropyrimidinase Related Proteins (DRPs) have known homologous to the Collapsing Response Mediator Proteins (CRMPs). The DRP gene family has comprised four members, DRP 1, 2, 3, and 4, all out of which have considered to be involved in axonal outgrowth and path-finding.
  
  
  METHODS
  The protein has extracted from tumor, normal brain tissues, and then the protein purity has evaluated by Bradford test and spectrophotometric methods. In this study, proteins has separated by Two-Dimensional Gel (2DG) electrophoresis method and then spots have analyzed and compared using statistical data and specific software (Progenesis Same Spots).Spots have identified by pH isoelectric, molecular weights and data banks.
  
  
  RESULTS
  The 2D gel has shown 800 spots totally. Two spots have reported for DRP2, and one spot has reported for DRP3 in the human brain proteome, that have differed in pH isoelectric, and Molecular Weights values.
  
  
  CONCLUSIONS
  This protein family has involved in neuronal differentiation and axonal guidance, and abundantly influenced in the developing brain, but their expression persisted into adulthood. DRP2 has regulated by phosphorylation, Glycogen synthase kinase 3, regulate phosphorylation of DRP2 an inactive from, and induced neuronal polarity.
authors.affiliation: Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran ; Dept. of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Pooladi
authors.affiliation: Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Rezaei-Tavirani
authors.affiliation: Dept. of Molecular Genetics, Tehran Medical Branch, Islamic Azad University Tehran, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Hashemi
authors.affiliation: Dept. of Biology, Garmsar Branch, Islamic Azad University, Garmsar, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Hesami-Tackallou
authors.affiliation: Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran ; Dept. of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Solmaz
authors.initials: S
authors.lastname: Khaghani-Razi-Abad
authors.affiliation: Dept. of Pathology, Shohada Hospital, Shahid Beheshti University Of Medical Science, Tehran, Iran.
authors.forename: Afshin
authors.initials: A
authors.lastname: Moradi
authors.affiliation: Dept. of Neurosurgery, Shohada Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Zali
authors.affiliation: Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Masoumea
authors.initials: M
authors.lastname: Mousavi
authors.affiliation: Dept. of Pathology, Shohada Hospital, Shahid Beheshti University Of Medical Science, Tehran, Iran.
authors.forename: Azadeh
authors.initials: A
authors.lastname: Rakhshan
authors.affiliation: Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Firozi-Dalvand
authors.affiliation: Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Roghaye
authors.initials: R
authors.lastname: Omidi
keywords: Asterocytoma; DRP and 2DG Electrophoresis; Proteomics