title: Mitochondrial genomes of Trichinella species and genotypes – a basis  for diagnosis, and systematic and epidemiological explorations.
abstract: In the present study we sequenced or re-sequenced, assembled and annotated 15 mitochondrial genomes representing the 12 currently recognised taxa of Trichinella using a deep sequencing-coupled approach. We then defined and compared the gene order in individual mitochondrial genomes (14 to 17.7 kb), evaluated genetic differences among species/genotypes and re-assessed the relationships among these taxa using the mitochondrial nucleic acid or amino acid sequence data sets. In addition, a rich source of mitochondrial genetic markers was defined that could be used in future systematic, epidemiological and population genetic studies of Trichinella. The sequencing-bioinformatic approach employed herein should be applicable to a wide range of eukaryotic parasites.
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Namitha
authors.initials: N
authors.lastname: Mohandas
authors.forename: Edoardo
authors.initials: E
authors.lastname: Pozio
authors.forename: Giuseppe
authors.initials: G
authors.lastname: La Rosa
authors.forename: Pasi K
authors.initials: PK
authors.lastname: Korhonen
authors.forename: Neil D
authors.initials: ND
authors.lastname: Young
authors.forename: Anson V
authors.initials: AV
authors.lastname: Koehler
authors.forename: Ross S
authors.initials: RS
authors.lastname: Hall
authors.forename: Paul W
authors.initials: PW
authors.lastname: Sternberg
authors.forename: Peter R
authors.initials: PR
authors.lastname: Boag
authors.forename: Aaron R
authors.initials: AR
authors.lastname: Jex
authors.forename: Bill C H
authors.initials: BC
authors.lastname: Chang
authors.forename: Robin B
authors.initials: RB
authors.lastname: Gasser
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D049751:Genome, Helminth / Q000235:genetics*; D054629:Genome, Mitochondrial / Q000235:genetics*; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014234:Trichinella / Q000145:classification / Q000235:genetics*
chemical_list: D018509:DNA, Helminth; D004272:DNA, Mitochondrial