title: Y-box binding protein 1 is correlated with lymph node metastasis in intestinal-type gastric cancer.
abstract: OBJECTIVE
  Y-box binding protein-1 (YB-1) is known to modulate gene transcription and protein translation, as well as cellular response to drug treatment. The aim of this study is to correlate YB-1 protein expression levels with clinicopathological parameters in intestinal-type gastric cancer tissue samples (as categorized by the Lauren classification) and substantiate the findings with in vitro experimentation.
  
  
  RESULTS
  Paraffin-embedded samples from 167 patients with intestinal-type gastric cancer were used for the construction of tissue microarrays (TMAs). TMA slides were immunostained and YB-1 immunoreactivity score was based on the weighted average intensity score. Univariate analysis revealed that YB-1 immunohistochemical expression was correlated significantly with lymph node status (P = 0.054, borderline significance) and perforation (P = 0.043). YB-1 expression was also found to be an independent predictor of lymph node spread by multivariate analysis. Furthermore, siRNA-mediated YB-1 gene knockdown in MKN7 gastric cancer cells (which is known to originate from an intestinal-type gastric cancer tissue) inhibited cell migration (P = 0.0002) and invasion in vitro (P = 0.0129) significantly.
  
  
  CONCLUSIONS
  YB-1 expression is associated with lymph node spread in intestinal-type gastric cancer and is a potential prognostic biomarker in this subtype of gastric cancer.
authors.affiliation: Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
authors.forename: TianTian
authors.initials: T
authors.lastname: Guo
authors.forename: YingNan
authors.initials: Y
authors.lastname: Yu
authors.forename: George Wai-Cheong
authors.initials: GW
authors.lastname: Yip
authors.forename: Gyeong Hun
authors.initials: GH
authors.lastname: Baeg
authors.forename: Aye Aye
authors.initials: AA
authors.lastname: Thike
authors.forename: Tony Kiat-Hon
authors.initials: TK
authors.lastname: Lim
authors.forename: Puay Hoon
authors.initials: PH
authors.lastname: Tan
authors.forename: Ken
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Boon Huat
authors.initials: BH
authors.lastname: Bay
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008198:Lymph Nodes / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D034741:RNA, Small Interfering; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D051840:Y-Box-Binding Protein 1 / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D034741:RNA, Small Interfering; D051840:Y-Box-Binding Protein 1
keywords: YB-1; cell invasion; cell migration; immunohistochemistry; lauren classification; lymph node spread