title: Hemodynamic flow visualization of early embryonic great vessels using μPIV.
abstract: Microparticle image velocimetry (μPIV) is an evolving quantitative methodology to closely and accurately monitor the cardiac flow dynamics and mechanotransduction during vascular morphogenesis. While PIV technique has a long history, contemporary developments in advanced microscopy have significantly expanded its power. This chapter includes three new methods for μPIV acquisition in selected embryonic structures achieved through advanced optical imaging: (1) high-speed confocal scanning of transgenic zebrafish embryos, where the transgenic erythrocytes act as the tracing particles; (2) microinjection of artificial seeding particles in chick embryos visualized with stereomicroscopy; and (3) real-time, time-resolved optical coherence tomography acquisition of vitelline vessel flow profiles in chick embryos, tracking the erythrocytes.
authors.affiliation: Department of Mechanical Engineering, Koc University, Istanbul, Turkey.
authors.forename: Selda
authors.initials: S
authors.lastname: Goktas
authors.forename: Chia-Yuan
authors.initials: CY
authors.lastname: Chen
authors.forename: William J
authors.initials: WJ
authors.lastname: Kowalski
authors.forename: Kerem
authors.initials: K
authors.lastname: Pekkan
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology*; D001808:Blood Vessels / Q000502:physiology*; D002642:Chick Embryo; D004625:Embryo, Nonmammalian / Q000098:blood supply*; D021621:Imaging, Three-Dimensional; D044085:Microfluidics; D008845:Microinjections; D018613:Microscopy, Confocal; D012212:Rheology / Q000379:methods*; D033081:Shear Strength; D059008:Time-Lapse Imaging; D041623:Tomography, Optical Coherence; D015027:Zebrafish / Q000196:embryology*