title: Antibacterial activity and inhibition of adherence of Streptococcus mutans by propolis electrospun fibers.
abstract: Mouth-dissolving fibers with antibacterial activity for the oral cavity were prepared by an electrospinning technique. Propolis extract was used as an active ingredient and polyvinylpyrrolidone (PVP) K90 as the polymer matrix. The morphology and diameter of the fibers were characterized by scanning electron microscopy. Antibacterial activity against Streptococcus mutans and the inhibition of S. mutans adhesion on a smooth glass surface during the biofilm formation were tested. Propolis, 5% (w/v), was combined with a PVP K90 solution, 8% (w/v), with or without Tween 80 including flavor additives and electrospun with an applied voltage of 15 kV. Uniform and smooth fibers of propolis-PVP K90 were obtained. The results showed that electrospun fibers with propolis extract can dissolve and release the propolis in water. Propolis-PVP electrospun fibers showed better antibacterial activity by reduction of bacteria adhesion on a smooth glass surface when compared to some commercial mouthwash products. These results indicated the potential of electrospun fibers to be used as mouth-dissolving fibers for effective antibacterial activity in the oral cavity.
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Suthep Rd., Muang district, Chiang Mai, 50200, Thailand.
authors.forename: Chawalinee
authors.initials: C
authors.lastname: Asawahame
authors.forename: Krit
authors.initials: K
authors.lastname: Sutjarittangtham
authors.forename: Sukum
authors.initials: S
authors.lastname: Eitssayeam
authors.forename: Yingmanee
authors.initials: Y
authors.lastname: Tragoolpua
authors.forename: Busaban
authors.initials: B
authors.lastname: Sirithunyalug
authors.forename: Jakkapan
authors.initials: J
authors.lastname: Sirithunyalug
mesh_terms: D000284:Administration, Oral; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D004339:Drug Compounding / Q000379:methods; D004355:Drug Stability; D004595:Electroplating / Q000379:methods*; D009067:Mouthwashes / Q000008:administration & dosage / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011429:Propolis / Q000008:administration & dosage* / Q000737:chemistry*; D012399:Rotation; D013295:Streptococcus mutans / Q000187:drug effects* / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D009067:Mouthwashes; D011429:Propolis