title: Retrotransposon-based molecular markers for analysis of genetic diversity within the Genus Linum.
abstract: SSAP method was used to study the genetic diversity of 22 Linum species from sections Linum, Adenolinum, Dasylinum, Stellerolinum, and 46 flax cultivars. All the studied flax varieties were distinguished using SSAP for retrotransposons FL9 and FL11. Thus, the validity of SSAP method was demonstrated for flax marking, identification of accessions in genebank collections, and control during propagation of flax varieties. Polymorphism of Fl1a, Fl1b, and Cassandra insertions were very low in flax varieties, but these retrotransposons were successfully used for the investigation of Linum species. Species clusterization based on SSAP markers was in concordance with their taxonomic division into sections Dasylinum, Stellerolinum, Adenolinum, and Linum. All species of sect. Adenolinum clustered apart from species of sect. Linum. The data confirmed the accuracy of the separation in these sections. Members of section Linum are not as closely related as members of other sections, so taxonomic revision of this section is desirable. L. usitatissimum accessions genetically distant from modern flax cultivars were revealed in our work. These accessions are of utmost interest for flax breeding and introduction of new useful traits into flax cultivars. The chromosome localization of Cassandra retrotransposon in Linum species was determined.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Nataliya V
authors.initials: NV
authors.lastname: Melnikova
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Anna V
authors.initials: AV
authors.lastname: Kudryavtseva
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Zelenin
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Valentina A
authors.initials: VA
authors.lastname: Lakunina
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Olga Yu
authors.initials: OY
authors.lastname: Yurkevich
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia ; Department of Higher Plants, Lomonosov Moscow State University, Moscow 119991, Russia.
authors.forename: Anna S
authors.initials: AS
authors.lastname: Speranskaya
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Dmitriev
authors.affiliation: Department of Higher Plants, Lomonosov Moscow State University, Moscow 119991, Russia.
authors.forename: Anastasia A
authors.initials: AA
authors.lastname: Krinitsina
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia ; Research Institute of Physico-Chemical Medicine, Moscow 119435, Russia.
authors.forename: Maxim S
authors.initials: MS
authors.lastname: Belenikin
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Leonid A
authors.initials: LA
authors.lastname: Uroshlev
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Anastasiya V
authors.initials: AV
authors.lastname: Snezhkina
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Asiya F
authors.initials: AF
authors.lastname: Sadritdinova
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Nadezda V
authors.initials: NV
authors.lastname: Koroban
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Alexandra V
authors.initials: AV
authors.lastname: Amosova
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Tatiana E
authors.initials: TE
authors.lastname: Samatadze
authors.affiliation: Institute of Genetics and Cytology, National Academy of Science of Belarus, 220072 Minsk, Belarus.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Guzenko
authors.affiliation: Institute of Genetics and Cytology, National Academy of Science of Belarus, 220072 Minsk, Belarus.
authors.forename: Valentina A
authors.initials: VA
authors.lastname: Lemesh
authors.affiliation: Research Center for Obstetrics, Gynecology and Perinatology, Moscow 117997, Russia.
authors.forename: Anastasya M
authors.initials: AM
authors.lastname: Savilova
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Olga A
authors.initials: OA
authors.lastname: Rachinskaia
authors.affiliation: All-Russian Research Institute for Flax of the Russian Academy of Agricultural Sciences, Torzhok 172002, Russia.
authors.forename: Natalya V
authors.initials: NV
authors.lastname: Kishlyan
authors.affiliation: All-Russian Research Institute for Flax of the Russian Academy of Agricultural Sciences, Torzhok 172002, Russia.
authors.forename: Tatiana A
authors.initials: TA
authors.lastname: Rozhmina
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Nadezhda L
authors.initials: NL
authors.lastname: Bolsheva
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Muravenko
mesh_terms: D016172:DNA Fingerprinting; D015342:DNA Probes / Q000378:metabolism; D060146:Ecotype; D019597:Flax / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D018745:Genome, Plant / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D010802:Phylogeny; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results; D018626:Retroelements / Q000235:genetics*; D013045:Species Specificity
chemical_list: D015342:DNA Probes; D005819:Genetic Markers; D018626:Retroelements