title: Flavobacterium arsenitoxidans sp. nov., an arsenite-oxidizing bacterium from Thai soil.
abstract: An arsenite-oxidizing bacterium, strain S2-3H(T), was isolated from arsenic-contaminated soil sample collected from Dantchaeng district, Suphanburi province, Thailand and was characterized based on polyphasic taxonomic study. The strain was observed to be a Gram-stain negative, aerobic, yellow pigmented, non-spore forming and rod-shaped bacterium. Major menaquinone was MK-6. Iso-C15:0, iso-C15:0 3OH, C16:1 ω7c/C16:1 ω6c, C16:0, iso-C17:0 3OH, and C16:0 3OH were the predominant cellular fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, unidentified phospholipids and unidentified aminophospholipids. The DNA G+C content was 37.0 mol%. Phylogenetic analysis using 16S rRNA sequence showed that strain S2-3H(T) is affiliated to the genus Flavobacterium, and is closely related to F. defluvii KCTC 12612(T) (97.0 %) and F. johnsoniae NBRC 14942(T) (97.0 %). The strain S2-3H(T) could be clearly distinguished from the related Flavobacterium species by its physiological and biochemical characteristics as well as its phylogenetic position and DNA-DNA relatedness. Therefore, the strain represents a novel species of the genus Flavobacterium, for which the name Flavobacterium arsenitoxidans sp. nov. (type strain S2-3H(T) = KCTC 22507(T) = NBRC 109607(T) = PCU 331(T) = TISTR 2238(T)) is proposed.
authors.affiliation: Faculty of Animal Sciences and Agricultural Technology, Silpakorn University, Phetchaburi IT Campus, Phetchaburi, 76120, Thailand.
authors.forename: Saowapar
authors.initials: S
authors.lastname: Khianngam
authors.forename: Ancharida
authors.initials: A
authors.lastname: Akaracharanya
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Lee
authors.forename: Keun Chul
authors.initials: KC
authors.lastname: Lee
authors.forename: Kyoung-Woong
authors.initials: KW
authors.lastname: Kim
authors.forename: Somboon
authors.initials: S
authors.lastname: Tanasupawat
mesh_terms: D000332:Aerobiosis; D018053:Arsenites / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013785:Thailand; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D018053:Arsenites; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C015001:arsenite