title: Antimicrobial activity of toothpastes containing natural extracts, chlorhexidine or triclosan.
abstract: The objective of this in vitro study was to evaluate the antimicrobial effect of toothpastes containing natural extracts, chlorhexidine or triclosan. The effectiveness of toothpastes containing natural extracts (Parodontax®), 0.12% chlorhexidine (Cariax®), 0.3% triclosan (Sanogil®) or fluoride (Sorriso®, control) was evaluated against yeasts, Gram-positive and Gram-negative bacteria using the disk diffusion method. Water was used as a control. Disks impregnated with the toothpastes were placed in Petri dishes containing culture media inoculated with 23 indicative microorganisms by the pour plate method. After incubation, the inhibition growth halos were measured and statistical analyses (α=0.05) were performed. The results indicated that all formulations, except for conventional toothpaste (Sorriso®), showed antimicrobial activity against Gram-positive bacteria and yeasts. The toothpaste containing natural extracts (Parodontax®) was the only product able to inhibit the growth of Pseudomonas aeruginosa. The toothpastes containing chlorhexidine, triclosan or natural extracts presented antimicrobial activity against Gram-positive bacteria and yeasts.
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Andiara
authors.initials: A
authors.lastname: De Rossi
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Danielly Cunha Araújo
authors.initials: DC
authors.lastname: Ferreira
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Raquel Assed Bezerra
authors.initials: RA
authors.lastname: da Silva
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Alexandra Mussolino
authors.initials: AM
authors.lastname: de Queiroz
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Léa Assed Bezerra
authors.initials: LA
authors.lastname: da Silva
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Paulo
authors.initials: P
authors.lastname: Nelson-Filho
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001688:Biological Products / Q000494:pharmacology*; D002710:Chlorhexidine / Q000494:pharmacology*; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D014100:Toothpastes; D014260:Triclosan / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D001688:Biological Products; D014100:Toothpastes; D014260:Triclosan; D002710:Chlorhexidine