title: 'Candidatus Rickettsia asemboensis' and Wolbachia spp. in Ctenocephalides felis and Pulex irritans fleas removed from dogs in Ecuador.
abstract: BACKGROUND
  Flea-borne infections are distributed worldwide. Up to date there are no reports about microorganisms associated to fleas in Ecuador.
  
  
  METHODS
  Seventy-one Pulex irritans and 8 Ctenocephalides felis fleas were removed from dogs in two Ecuadorian areas (Pastaza and Chimborazo Provinces) in December 2012. DNA extracts were tested by polymerase chain reaction (PCR) assays targeting universal 16S rRNA, as well as screened for the presence of Rickettsia spp. (gltA, htrA, ompB, sca4 and ompA genes) and Bartonella spp. (rpoB, gltA and ITS genes).
  
  
  RESULTS
  Our results showed the presence of 'Candidatus Rickettsia asemboensis' (highly similar to R. felis) in C. felis and Wolbachia spp. endosimbionts in P. irritans collected from animals in Ecuador. No fleas were found to be positive for any Bartonella species or Yersinia pestis.
  
  
  CONCLUSIONS
  Clinicians should be aware of the potential risk of this new Candidatus Rickettsia sp. and keep in mind other flea-borne infections since these flea species frequently bite humans.
authors.affiliation: Departamento de Enfermedades Infecciosas, Hospital San Pedro-Centro de Investigación Biomédica de La Rioja (CIBIR), C/ Piqueras 98, Logroño (La Rioja), 26006, Spain. jaoteo@riojasalud.es.
authors.forename: José A
authors.initials: JA
authors.lastname: Oteo
authors.forename: Aránzazu
authors.initials: A
authors.lastname: Portillo
authors.forename: Francisco
authors.initials: F
authors.lastname: Portero
authors.forename: Jorge
authors.initials: J
authors.lastname: Zavala-Castro
authors.forename: José M
authors.initials: JM
authors.lastname: Venzal
authors.forename: Marcelo B
authors.initials: MB
authors.lastname: Labruna
mesh_terms: D000818:Animals; D001483:Base Sequence; D058251:Ctenocephalides / Q000382:microbiology; D004269:DNA, Bacterial / Q000737:chemistry; D004283:Dog Diseases / Q000382:microbiology* / Q000469:parasitology / Q000635:transmission; D004285:Dogs; D004484:Ecuador; D005260:Female; D058267:Flea Infestations / Q000469:parasitology / Q000662:veterinary*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012281:Rickettsia / Q000235:genetics / Q000302:isolation & purification*; D012282:Rickettsia Infections / Q000382:microbiology / Q000635:transmission; D012288:Rickettsiaceae Infections / Q000382:microbiology* / Q000635:transmission; D017422:Sequence Analysis, DNA; D005423:Siphonaptera / Q000382:microbiology*; D020577:Wolbachia / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S