title: Roles of bovine viral diarrhea virus envelope glycoproteins in inducing autophagy in MDBK cells.
abstract: Macroautophagy (autophagy) is an evolutionarily conserved control process that maintains cellular homeostasis in eukaryotic cells. Autophagy principally serves an adaptive role to degrade dysfunctional proteins and to clean damaged organelles in response to pathogenic, viral, or microbial infection, nutrient deprivation and endoplasmic reticulum (ER) stress. In previous study, we showed bovine viral diarrhea virus (BVDV) NADL infection induced autophagy and significantly elevated the expression levels of autophagy-related genes, Beclin1 and ATG14, at 12 h post-infection in MDBK cells. However, the specific mechanisms involved in controlling autophagic activity remain unclear. Here, we investigate the effects of BVDV NADL envelope glycoproteins overexpression on inducing autophagy. The results show that viral envelope glycoproteins E(rns) and E2 overexpression mediated by lentivirus increase the formation of autophagosome, the percentage of GFP-LC3 puncta-positive cells and the expression levels of Beclin1 and ATG14. Whereas E1 overexpression doesn't affect autophagic activity. Collectively, these findings suggest that the viral envelope glycoproteins E(rns) and E2 are involved in inducing autophagy, and provide a mechanistic insight into the regulation of autophagy in viral infected cells.
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Shi
authors.affiliation: College of Life Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Mengting
authors.initials: M
authors.lastname: Shi
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Luping
authors.initials: L
authors.lastname: Meng
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Bao
authors.affiliation: College of Life Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Guoqi
authors.initials: G
authors.lastname: Zhang
authors.affiliation: College of Medicine, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ren
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Medicine, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Qiao
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Jia
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Pengyan
authors.initials: P
authors.lastname: Wang
authors.affiliation: College of Life Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ni
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China.
authors.forename: Jinliang
authors.initials: J
authors.lastname: Sheng
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China. Electronic address: cfchen1964@gmail.com.
authors.forename: Chuangfu
authors.initials: C
authors.lastname: Chen
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000032:analysis; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000032:analysis; D001343:Autophagy; D002417:Cattle; D002460:Cell Line; D001908:Diarrhea Viruses, Bovine Viral / Q000502:physiology*; D020869:Gene Expression Profiling; D006023:Glycoproteins / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D014759:Viral Envelope Proteins / Q000378:metabolism*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D051017:Apoptosis Regulatory Proteins; D006023:Glycoproteins; D014759:Viral Envelope Proteins
keywords: Autophagy; Bovine viral diarrhea virus; Viral envelope glycoproteins E(rns), E1 and E2