title: Exploring microRNA-mediated alteration of EGFR signaling pathway in non-small cell lung cancer using an mRNA:miRNA regression model supported by target prediction databases.
abstract: EGFR signaling pathway and microRNAs (miRNAs) are two important factors for development and treatment in non-small cell lung cancer (NSCLC). Microarray analysis enables the genome-wide expression profiling. However, the information from microarray data may not be fully deciphered through the existing approaches. In this study we present an mRNA:miRNA stepwise regression model supported by miRNA target prediction databases. This model is applied to explore the roles of miRNAs in the EGFR signaling pathway. The results show that miR-145 is positively associated with epidermal growth factor (EGF) in the pre-surgery NSCLC group and miR-199a-5p is positively associated with EGF in the post-surgery NSCLC group. Surprisingly, miR-495 is positively associated with protein tyrosine kinase 2 (PTK2) in both groups. The coefficient of determination (R(2)) and leave-one-out cross-validation (LOOCV) demonstrate good performance of our regression model, indicating that it can identify the miRNA roles as oncomirs and tumor suppressor mirs in NSCLC.
authors.affiliation: Department of Health Technology and Informatics, Hong Kong Polytechnic University, Hong Kong, China.
authors.forename: Fengfeng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Health Technology and Informatics, Hong Kong Polytechnic University, Hong Kong, China. Electronic address: wing.chi.chan@polyu.edu.hk.
authors.forename: Lawrence W C
authors.initials: LW
authors.lastname: Chan
authors.affiliation: Department of Health Technology and Informatics, Hong Kong Polytechnic University, Hong Kong, China.
authors.forename: Helen K W
authors.initials: HK
authors.lastname: Law
authors.affiliation: Department of Clinical Oncology, Queen Elizabeth Hospital, Hong Kong, China.
authors.forename: William C S
authors.initials: WC
authors.lastname: Cho
authors.affiliation: Bioinformatics Center, Chang Gung University, Taiwan.
authors.forename: Petrus
authors.initials: P
authors.lastname: Tang
authors.affiliation: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
authors.affiliation: Informatics Institute and Department of Computer Science, University of Missouri, Columbia, MO, USA.
authors.forename: Chi-Ren
authors.initials: CR
authors.lastname: Shyu
authors.affiliation: Department of Health Technology and Informatics, Hong Kong Polytechnic University, Hong Kong, China.
authors.forename: S C Cesar
authors.initials: SC
authors.lastname: Wong
authors.affiliation: Department of Health Technology and Informatics, Hong Kong Polytechnic University, Hong Kong, China.
authors.forename: S P
authors.initials: SP
authors.lastname: Yip
authors.affiliation: Department of Health Technology and Informatics, Hong Kong Polytechnic University, Hong Kong, China.
authors.forename: Benjamin Y M
authors.initials: BY
authors.lastname: Yung
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism*; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D051417:Focal Adhesion Kinase 1 / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics*; D008957:Models, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012044:Regression Analysis; D015398:Signal Transduction
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D066246:ErbB Receptors; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human
keywords: EGFR signaling pathway; Microarray analysis; Non-small cell lung cancer; Regression model; miRNA