title: SLC30A3 and SEP15 gene polymorphisms influence the serum concentrations of zinc and selenium in mature adults.
abstract: Because of their numerous roles in several biological processes, zinc and selenium are the most commonly studied micronutrients in the elderly. Therefore, we hypothesized that the polymorphisms in the genes that are responsible for the transport of zinc and selenium may have a genotype-dependent effect on the serum concentration of these micronutrients. The objective of this study was to determine the effects of solute carrier family 30 member 3 (SLC30A3) and 15-kd selenoprotein (SEP15) polymorphisms on zinc and selenium concentrations, respectively, in the serum. This cross-sectional study included 110 individuals who were aged 50 years or older. Serum micronutrient concentrations were determined by flame atomic absorption spectrophotometry (for zinc) and by atomic absorption spectrophotometry with a graphite furnace (for selenium). The single-nucleotide polymorphisms, rs73924411 and rs11126936 of the SLC30A3 gene and rs5859, rs5854, and rs561104 of the SEP15 gene, were examined by real-time polymerase chain reaction. Regarding rs11126936, the serum zinc concentration was lower in CC homozygotes (0.75 ± 0.31 mg/L) than in A carriers (0.89 ± 0.28 mg/L, P = .016). Concerning rs561104, the serum selenium concentration was higher in CC homozygotes (5.65 ± 1.11 μg/dL) compared with T carriers (4.88 ± 1.25 μg/dL, P = .044). Our results demonstrate the influence of SLC30A3 and SEP15 gene polymorphisms on the serum concentrations of zinc and selenium, respectively. The effects of these associations should be further investigated to help elucidate the modes of action of trace elements and to identify biomarkers, which could ultimately define the optimal intake of these micronutrients at the molecular level. More research must be performed before the roles of these polymorphisms in the serum concentrations of zinc and selenium can be fully understood.
authors.affiliation: Biomedical Health Sciences, University of Health Sciences of Porto Alegre-UFCSPA, Rio Grande do Sul, Brazil. Electronic address: jacobsen.tati@gmail.com.
authors.forename: Tatiane Jacobsen
authors.initials: TJ
authors.lastname: da Rocha
authors.affiliation: Biomedicine, Institute of Health Sciences, University Feevale, Rio Grande do Sul, Brazil. Electronic address: milakorb@gmail.com.
authors.forename: Camila
authors.initials: C
authors.lastname: Korb
authors.affiliation: Biomedical Science, Feevale University, Rio Grande do Sul, Brazil. Electronic address: jaqbs@ibest.com.br.
authors.forename: Jaqueline Bohrer
authors.initials: JB
authors.lastname: Schuch
authors.affiliation: Psychology, Feevale University, Rio Grande do Sul, Brazil. Electronic address: bamberg.daiani@gmail.com.
authors.forename: Daiani Pires
authors.initials: DP
authors.lastname: Bamberg
authors.affiliation: Institute of Sciences, Letters and Arts and Institute of Health Sciences, University Feevale, Rio Grande do Sul, Brazil. Electronic address: fabiana.andrade@feevale.br.
authors.forename: Fabiana Michelsen
authors.initials: FM
authors.lastname: de Andrade
authors.affiliation: Department of Basic Health Sciences, UFCSPA, Rio Grande do Sul, Brazil. Electronic address: mariluf@ufcspa.edu.br.
authors.forename: Marilu
authors.initials: M
authors.lastname: Fiegenbaum
mesh_terms: D000368:Aged; D027682:Cation Transport Proteins / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009752:Nutritional Status / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D012643:Selenium / Q000097:blood* / Q000172:deficiency; D051140:Selenoproteins / Q000235:genetics*; D014131:Trace Elements / Q000097:blood* / Q000172:deficiency; D015032:Zinc / Q000097:blood* / Q000172:deficiency
chemical_list: D027682:Cation Transport Proteins; C495238:SELENOF protein, human; C102806:SLC30A3 protein, human; D051140:Selenoproteins; D014131:Trace Elements; D012643:Selenium; D015032:Zinc
keywords: Aging; Polymorphism; SEP15; SLC30A3; Selenium; Zinc