title: Antibacterial power of sodium hypochlorite combined with surfactants and acetic acid.
abstract: The aim of this ex vivo study was to compare the antibacterial power of 1% NaOCl with 1% acetic acid, 5.25% NaOCl and two commercially available NaOCl modified with surfactants in bovine root dentin. A total of 120 dentin tubes prepared from intact bovine incisors were infected for 21 days with Enterococcus faecalis and randomly divided into six groups as follows: 5.25%NaOCl; Hypoclean; Chlor-Xtra; 1% NaOCl with 1% acetic acid; infected dentin tubes (positive control); and sterile dentin tubes (negative control). At experimental times of 0, 7, 14, 21 and 28 days, dentin chips were collected using sequential round burs with increasing diameters in separate test tubes containing 3 mL of freshly prepared BHI. Statistical analysis were performed using parametric methods (one-way ANOVA, and Bonferroni's multiple comparisons test, α=0.01). After culturing, the number of colony-forming units (CFU) was counted. All the NaOCl solutions showed small number of CFU over 28 days. ChlorXtra and Hypoclean had the smallest number of CFU at all times with greater antimicrobial efficacy than 5.25% NaOCl and 1% NaOCl solution with 1% acetic acid.
authors.affiliation: Department of Endodontics, School of Dentistry, Università degli Studi di Torino, Crotone, Italy.
authors.forename: Luciano
authors.initials: L
authors.lastname: Giardino
authors.affiliation: Department of Stomatological Sciences, School of Dentistry, UFG - Federal University of Goiás, Goiânia, GO, Brazil.
authors.forename: Carlos
authors.initials: C
authors.lastname: Estrela
authors.affiliation: Center for Endodontic Research (ICER), Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Zahed
authors.initials: Z
authors.lastname: Mohammadi
authors.affiliation: Private Practice, Naples, Italy.
authors.forename: Flavio
authors.initials: F
authors.lastname: Palazzi
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D013501:Surface-Active Agents / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D013501:Surface-Active Agents; D012973:Sodium Hypochlorite; D019342:Acetic Acid