title: Improving product yields on D-glucose in Escherichia coli via knockout of pgi and zwf and feeding of supplemental carbon sources.
abstract: The use of lignocellulosic biomass as a feedstock for microbial fermentation processes presents an opportunity for increasing the yield of bioproducts derived directly from glucose. Lignocellulosic biomass consists of several fermentable sugars, including glucose, xylose, and arabinose. In this study, we investigate the ability of an E. coli Δpgi Δzwf mutant to consume alternative carbon sources (xylose, arabinose, and glycerol) for growth while reserving glucose for product formation. Deletion of pgi and zwf was found to eliminate catabolite repression as well as the ability of E. coli to consume glucose for biomass formation. In addition, the yield from glucose of the bioproduct D-glucaric acid was significantly increased in a Δpgi Δzwf strain.
authors.affiliation: Department of Chemical Engineering, Synthetic Biology Engineering Research Center (SynBERC), Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139.
authors.forename: Eric
authors.initials: E
authors.lastname: Shiue
authors.forename: Irene M
authors.initials: IM
authors.lastname: Brockman
authors.forename: Kristala L J
authors.initials: KL
authors.lastname: Prather
mesh_terms: D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D018929:Cell Culture Techniques / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005285:Fermentation; D055786:Gene Knockout Techniques; D005937:Glucaric Acid / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics*; D060847:Metabolic Engineering / Q000379:methods*
chemical_list: D029968:Escherichia coli Proteins; D005956:Glucose-6-Phosphate Isomerase; C554958:pgi protein, E coli; D005947:Glucose; D005937:Glucaric Acid
keywords: D-glucaric acid; biomass; product yield; strain engineering