title: Activated Microglia-Induced Deficits in Excitatory Synapses Through IL-1β: Implications for Cognitive Impairment in Sepsis.
abstract: Recent clinical studies have shown that sepsis survivors may develop long-term cognitive impairments. The cellular and molecular mechanisms involved in these events are not well understood. This study investigated synaptic deficits in sepsis and the involvement of glial cells in this process. Septic animals showed memory impairment and reduced numbers of hippocampal and cortical excitatory synapses, identified by synaptophysin/PSD-95 co-localization, 9 days after disease onset. The behavioral deficits and synaptophysin/PSD-95 co-localization were rescued to normal levels within 30 days post-sepsis. Septic mice presented activation of microglia and reactive astrogliosis, which are hallmarks of brain injury and could be involved in the associated synaptic deficits. We treated neuronal cultures with conditioned medium derived from cultured astrocytes (ACM) and microglia (MCM) that were either non-stimulated or stimulated with lipopolysaccharide (LPS) to investigate the molecular mechanisms underlying synaptic deficits in sepsis. ACM and MCM increased the number of synapses between cortical neurons in vitro, and these effects were antagonized by LPS stimulation. LPS-MCM reduced the number of synapses by 50%, but LPS-ACM increased the number of synapses by 500%. Analysis of the composition of these conditioned media revealed increased levels of IL-1β in LPS-MCM. Furthermore, inhibition of IL-1β signaling through the addition of a soluble IL-1β receptor antagonist (IL-1 Ra) fully prevented the synaptic deficit induced by LPS-MCM. These results suggest that sepsis induces a transient synaptic deficit associated with memory impairments mediated by IL-1β secreted by activated microglia.
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, 21941-902, Rio de Janeiro, RJ, Brazil.
authors.forename: Carolina A
authors.initials: CA
authors.lastname: Moraes
authors.forename: Gabriel
authors.initials: G
authors.lastname: Santos
authors.forename: Tania Cristina Leite
authors.initials: TC
authors.lastname: de Sampaio e Spohr
authors.forename: Joana C
authors.initials: JC
authors.lastname: D'Avila
authors.forename: Flávia Regina Souza
authors.initials: FR
authors.lastname: Lima
authors.forename: Claudia Farias
authors.initials: CF
authors.lastname: Benjamim
authors.forename: Fernando A
authors.initials: FA
authors.lastname: Bozza
authors.forename: Flávia Carvalho Alcantara
authors.initials: FC
authors.lastname: Gomes
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects / Q000473:pathology; D003072:Cognition Disorders / Q000209:etiology* / Q000473:pathology; D005911:Gliosis / Q000209:etiology / Q000473:pathology; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology; D053583:Interleukin-1beta / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008569:Memory Disorders / Q000209:etiology / Q000473:pathology; D051379:Mice; D017628:Microglia / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D018805:Sepsis / Q000150:complications* / Q000473:pathology; D013569:Synapses / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*
chemical_list: D053583:Interleukin-1beta; D008070:Lipopolysaccharides