title: Biological mechanisms determining the success of RNA interference in insects.
abstract: Insects constitute the largest group of animals on this planet, having a huge impact on our environment, as well as on our quality of life. RNA interference (RNAi) is a posttranscriptional gene silencing mechanism triggered by double-stranded (ds)RNA fragments. This process not only forms the basis of a widely used reverse genetics research method in many different eukaryotes but also holds great promise to contribute to the species-specific control of agricultural pests and to combat viral infections in beneficial and disease vectoring insects. However, in many economically important insect species, such as flies, mosquitoes, and caterpillars, systemic delivery of naked dsRNA does not trigger effective gene silencing. Although many components of the RNAi pathway have initially been deciphered in the fruit fly, Drosophila melanogaster, it will be of major importance to investigate this process in a wider variety of species, including dsRNA-sensitive insects such as locusts and beetles, to elucidate the factors responsible for the remarkable variability in RNAi efficiency, as observed in different insects. In this chapter, we review the current knowledge on the RNAi pathway, as well as the most recent insights into the mechanisms that might determine successful RNAi in insects.
authors.affiliation: Department of Animal Physiology and Neurobiology, KU Leuven, Naamsestraat, Leuven, Belgium. Electronic address: niels.wynant@bio.kuleuven.be.
authors.forename: Niels
authors.initials: N
authors.lastname: Wynant
authors.affiliation: Department of Animal Physiology and Neurobiology, KU Leuven, Naamsestraat, Leuven, Belgium.
authors.forename: Dulce
authors.initials: D
authors.lastname: Santos
authors.affiliation: Department of Animal Physiology and Neurobiology, KU Leuven, Naamsestraat, Leuven, Belgium.
authors.forename: Jozef
authors.initials: J
authors.lastname: Vanden Broeck
mesh_terms: D000818:Animals; D007313:Insecta / Q000235:genetics* / Q000378:metabolism*; D034622:RNA Interference / Q000502:physiology*; D012330:RNA, Double-Stranded / Q000235:genetics* / Q000378:metabolism*
chemical_list: D012330:RNA, Double-Stranded
keywords: Drosophila; Insect; Locust; Pest control; RNA interference; Systemic; Virus; dsRNA