title: miR-125b-1-3p inhibits trophoblast cell invasion by targeting sphingosine-1-phosphate receptor 1 in preeclampsia.
abstract: Preeclampsia (PE) is the leading cause of maternal and perinatal mortality and morbidity. Understanding the molecular mechanisms underlying placentation facilitates the development of better intervention of this disease. MicroRNAs are strongly implicated in the pathogenesis of this syndrome. In current study, we found that miR-125b-1-3p was elevated in placentas derived from preeclampsia patients. Transfection of miR-125b-1-3p mimics significantly inhibited the invasiveness of human trophoblast cells, whereas miR-125b-1-3p inhibitor enhanced trophoblast cell invasion. Luciferase assays identified that S1PR1 was a novel direct target of miR-125b-1-3p in the placenta. Overexpression of S1PR1 could reverse the inhibitory effect of miR-125b-1-3p on the invasion of trophoblast cells. These findings suggested that abnormal expression of miR-125b-1-3p might contribute to the pathogenesis of preeclampsia.
authors.affiliation: Public Health College, Weifang Medical University, Weifang 261053, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of Biological Sciences, Weifang Medical University, Weifang 261053, China.
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Pan
authors.affiliation: School of Biological Sciences, Weifang Medical University, Weifang 261053, China.
authors.forename: Xuejian
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Biological Sciences, Weifang Medical University, Weifang 261053, China.
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Gao
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Weifang Medical University, Weifang 261041, China.
authors.forename: Chune
authors.initials: C
authors.lastname: Ren
authors.affiliation: School of Biological Sciences, Weifang Medical University, Weifang 261053, China. Electronic address: yangweiwei0127@gmail.com.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Yang
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D005260:Female; D015870:Gene Expression; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism / Q000473:pathology; D010929:Placentation / Q000235:genetics / Q000502:physiology; D011225:Pre-Eclampsia / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D049349:Receptors, Lysosphingolipid / Q000235:genetics / Q000378:metabolism*; D000081025:Sphingosine-1-Phosphate Receptors; D014327:Trophoblasts / Q000378:metabolism* / Q000473:pathology*
chemical_list: C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D049349:Receptors, Lysosphingolipid; C477362:S1PR1 protein, human; D000081025:Sphingosine-1-Phosphate Receptors
keywords: Invasion; Preeclampsia; S1PR1; Trophoblast; miR-125b-1-3p