title: [DNA barcoding identification between arisaematis rhizoma and its adulterants based on ITS2 sequences].
abstract: Fifty-eight samples belonging to 7 species of Arisaematis Rhizoma and its adulterants were collected. The ITS2 locus was employed as a DNA barcode and amplified, sequenced and assembled for all of the collected samples. Then, ITS2 sequences have been annotated using HMM-based method. The intra- and inter-specific variations were calculated and NJ tree was constructed using MEGA 6.0 software. The results showed that inter-specific K2P distances were significantly larger than intra-specific distances for all of the three origin species of Arisaematis Rhizoma. Furthermore, three origin species, Arisaema amurense, A. erubescens and A. heterophyllum, can be respectively formed to be a single branch with high bootstrap values. It is concluded that ITS2 can be used to correctly identify Arisaematis Rhizoma from its adulterants and the application of ITS2 in the identification of traditional Chinese medicine has an important prospective.
authors.forename: Lin-Chun
authors.initials: LC
authors.lastname: Shi
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Xiang
authors.forename: Jing-Yuan
authors.initials: JY
authors.lastname: Song
authors.forename: Hui
authors.initials: H
authors.lastname: Yao
mesh_terms: D031057:Arisaema / Q000145:classification* / Q000235:genetics; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D018744:DNA, Plant / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D004340:Drug Contamination / Q000517:prevention & control; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000145:classification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011786:Quality Control; D027343:Rhizome / Q000145:classification / Q000235:genetics
chemical_list: D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer; D004365:Drugs, Chinese Herbal