title: Examination of galectin-induced lattice formation on early B-cell development.
abstract: Galectin-1 (GAL1) is a pre-B cell receptor (pre-BCR) ligand that induces pre-BCR clustering and leads to efficient pre-B cell proliferation and differentiation in the bone marrow. To study pre-BCR-GAL1 interactions and its functional consequence on the early steps of the B cell development, we combine structural nuclear magnetic resonance (NMR) approaches and B cell biology techniques. NMR is applied to identify the residues involved in pre-BCR-GAL1 interactions by monitoring chemical shift perturbations when the complex is formed. This structural information is then used at the cellular level to target specifically the complex formation during GAL1-induced pre-BCR clustering and lattice formation, using immunofluorescence techniques. Moreover, an in vivo assay was set up to study the consequence of synapse formation on the early steps of B cell development.
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, INSERM U1104, CNRS UMR7280, case 906, Aix-Marseille Université, UM2, 13288, Marseille cedex 09, France.
authors.forename: Stéphane J C
authors.initials: SJ
authors.lastname: Mancini
authors.forename: Latifa
authors.initials: L
authors.lastname: Elantak
authors.forename: Annie
authors.initials: A
authors.lastname: Boned
authors.forename: Marion
authors.initials: M
authors.lastname: Espéli
authors.forename: Françoise
authors.initials: F
authors.lastname: Guerlesquin
authors.forename: Claudine
authors.initials: C
authors.lastname: Schiff
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology*; D001854:Bone Marrow Cells / Q000166:cytology; D002460:Cell Line; D005455:Fluorescent Antibody Technique / Q000379:methods*; D037483:Galectin 1 / Q000378:metabolism*; D006801:Humans; D054420:Pre-B Cell Receptors / Q000378:metabolism
chemical_list: D037483:Galectin 1; D054420:Pre-B Cell Receptors