title: Unaltered regulatory B-cell frequency and function in patients with multiple sclerosis.
abstract: Multiple sclerosis (MS) is a chronic disease of the central nervous system (CNS) typically characterized by the recruitment of T cells into the CNS. However, certain subsets of B cells have been shown to negatively regulate autoimmune diseases and some data support a prominent role for B cells in MS physiopathology. For B cells in MS patients we analyzed subset frequency, cytokine secretion ability and suppressive properties. No differences in the frequencies of the B-cell subsets or in their ability to secrete cytokines were observed between MS and healthy volunteers (HV). Prestimulated B cells from MS patients also inhibited CD4(+)CD25(-) T cell proliferation with a similar efficiency as B cells from HV. Altogether, our data show that, in our MS patient cohort, regulatory B cells have conserved frequency and function.
authors.affiliation: INSERM, UMR 1064, Nantes, F-44093, France; CHU Nord Laennec, Service de Neurologie, Nantes, F-44093, France; INSERM, CIC 004, Nantes, F-44093, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Michel
authors.affiliation: INSERM, UMR 1064, Nantes, F-44093, France; CHU de Nantes, ITUN, Nantes, F-44093, France.
authors.forename: Melanie
authors.initials: M
authors.lastname: Chesneau
authors.affiliation: INSERM, UMR 1064, Nantes, F-44093, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Manceau
authors.affiliation: INSERM, UMR 1064, Nantes, F-44093, France.
authors.forename: Athenais
authors.initials: A
authors.lastname: Genty
authors.affiliation: INSERM, UMR 1064, Nantes, F-44093, France.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Garcia
authors.affiliation: INSERM, UMR 1064, Nantes, F-44093, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Salou
authors.affiliation: INSERM, UMR 1064, Nantes, F-44093, France.
authors.forename: Annie
authors.initials: A
authors.lastname: Elong Ngono
authors.affiliation: INSERM, UMR 1064, Nantes, F-44093, France; CHU de Nantes, ITUN, Nantes, F-44093, France.
authors.forename: Annaïck
authors.initials: A
authors.lastname: Pallier
authors.affiliation: CHU Nord Laennec, Service de Neurologie, Nantes, F-44093, France; INSERM, CIC 004, Nantes, F-44093, France.
authors.forename: Marylène
authors.initials: M
authors.lastname: Jacq-Foucher
authors.affiliation: CHU Nord Laennec, Service de Neurologie, Nantes, F-44093, France; INSERM, CIC 004, Nantes, F-44093, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Lefrère
authors.affiliation: CHU Nord Laennec, Service de Neurologie, Nantes, F-44093, France; INSERM, CIC 004, Nantes, F-44093, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Wiertlewski
authors.affiliation: INSERM, UMR 1064, Nantes, F-44093, France; Universite de Nantes, Faculte de Medecine, Nantes, F-44035, France; CHU de Nantes, ITUN, Nantes, F-44093, France.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Soulillou
authors.affiliation: INSERM, UMR 1064, Nantes, F-44093, France; Universite de Nantes, Faculte de Medecine, Nantes, F-44035, France; CHU de Nantes, ITUN, Nantes, F-44093, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Degauque
authors.affiliation: INSERM, UMR 1064, Nantes, F-44093, France; CHU Nord Laennec, Service de Neurologie, Nantes, F-44093, France; INSERM, CIC 004, Nantes, F-44093, France; Universite de Nantes, Faculte de Medecine, Nantes, F-44035, France.
authors.forename: David-Axel
authors.initials: DA
authors.lastname: Laplaud
authors.affiliation: INSERM, UMR 1064, Nantes, F-44093, France; Universite de Nantes, Faculte de Medecine, Nantes, F-44035, France; CHU de Nantes, ITUN, Nantes, F-44093, France. Electronic address: Sophie.brouard@univ-nantes.fr.
authors.forename: Sophie
authors.initials: S
authors.lastname: Brouard
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000954:Antigens, Surface / Q000378:metabolism; D060151:B-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D023201:CD40 Ligand / Q000378:metabolism; D016022:Case-Control Studies; D002450:Cell Communication / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000175:diagnosis / Q000276:immunology* / Q000378:metabolism; D009841:Oligonucleotides / Q000276:immunology / Q000494:pharmacology; D010641:Phenotype; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D055815:Young Adult
chemical_list: D000954:Antigens, Surface; D016207:Cytokines; D009841:Oligonucleotides; D023201:CD40 Ligand
keywords: B cells; Multiple sclerosis; Regulation