title: Cell adhesion to plasma-coated PVC.
abstract: To produce environments suitable for cell culture, thin polymer films were deposited onto commercial PVC plates from radiofrequency acetylene-argon plasmas. The proportion of argon in the plasmas, P(Ar), was varied from 5.3 to 65.8%. The adhesion and growth of Vero cells on the coated surfaces were examined for different incubation times. Cytotoxicity tests were performed using spectroscopic methods. Carbon, O, and N were detected in all the samples using XPS. Roughness remained almost unchanged in the samples prepared with 5.3 and 28.9% but tended to increase for the films deposited with P(Ar) between 28.9 and 55.3%. Surface free energy increased with increasing P(Ar), except for the sample prepared at 28.9% of Ar, which presented the least reactive surface. Cells proliferated on all the samples, including the bare PVC. Independently of the deposition condition there was no evidence of cytotoxicity, indicating the viability of such coatings for designing biocompatible devices.
authors.affiliation: Laboratório de Plasmas Tecnológicos, Universidade Estadual Paulista (UNESP), Avenida Três de Março 511, 18087-180 Sorocaba, SP, Brazil.
authors.forename: Elidiane C
authors.initials: EC
authors.lastname: Rangel
authors.affiliation: Laboratório de Plasmas Tecnológicos, Universidade Estadual Paulista (UNESP), Avenida Três de Março 511, 18087-180 Sorocaba, SP, Brazil.
authors.forename: Eduardo S
authors.initials: ES
authors.lastname: de Souza
authors.affiliation: Laboratório de Plasmas Tecnológicos, Universidade Estadual Paulista (UNESP), Avenida Três de Março 511, 18087-180 Sorocaba, SP, Brazil.
authors.forename: Francine S
authors.initials: FS
authors.lastname: de Moraes
authors.affiliation: Laboratório de Biomateriais, Departamento de Ciências Fisiológicas, Pontifícia Universidade Católica, Praça Dr. José Ermírio de Moraes 290, 18030-095 Sorocaba, SP, Brazil.
authors.forename: Eliana A R
authors.initials: EA
authors.lastname: Duek
authors.affiliation: Laboratório de Biomateriais, Departamento de Ciências Fisiológicas, Pontifícia Universidade Católica, Praça Dr. José Ermírio de Moraes 290, 18030-095 Sorocaba, SP, Brazil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Lucchesi
authors.affiliation: Laboratório de Superfícies e Interfaces, Departamento de Física, Universidade Federal do Paraná, 81531-990 Curitiba, PR, Brazil.
authors.forename: Wido H
authors.initials: WH
authors.lastname: Schreiner
authors.affiliation: Laboratório de Plasmas Tecnológicos, Universidade Estadual Paulista (UNESP), Avenida Três de Março 511, 18087-180 Sorocaba, SP, Brazil.
authors.forename: Steven F
authors.initials: SF
authors.lastname: Durrant
authors.affiliation: Laboratório de Plasmas Tecnológicos, Universidade Estadual Paulista (UNESP), Avenida Três de Março 511, 18087-180 Sorocaba, SP, Brazil.
authors.forename: Nilson C
authors.initials: NC
authors.lastname: Cruz
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002522:Chlorocebus aethiops; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000378:metabolism*; D011143:Polyvinyl Chloride / Q000737:chemistry* / Q000378:metabolism*; D013499:Surface Properties; D014709:Vero Cells
chemical_list: D020099:Coated Materials, Biocompatible; D011143:Polyvinyl Chloride