title: Suppressors of cytokine signaling 1 and 3 are upregulated in brain resident cells in response to virus-induced inflammation of the central nervous system via at least two distinctive pathways.
abstract: Suppressors of cytokine signaling (SOCS) proteins are intracellular proteins that inhibit cytokine signaling in a variety of cell types. A number of viral infections have been associated with SOCS upregulation; however, not much is known about the mechanisms regulating SOCS expression during viral infection. In this study, we used two pathologically distinct intracerebral (i.c.) infection models to characterize temporal and spatial aspects of SOCS expression in the virus-infected central nervous system (CNS), and by employing various knockout mouse models, we sought to identify regulatory mechanisms that may underlie a virus induced upregulation of SOCS in the CNS. We found that i.c. infection with either lymphocytic choriomeningitis virus (LCMV) or yellow fever virus (YF) results in gradual upregulation of SOCS1/3 mRNA expression peaking at day 7 postinfection (p.i.). In the LCMV model, SOCS mRNA was expressed in brain resident cells, including astrocytes and some neurons, and for SOCS1 in particular this upregulation was almost entirely mediated by gamma interferon (IFN-γ) produced by infiltrating T cells. After infection with YF, we also found SOCS expression to be upregulated in brain resident cells with a peak on day 7 p.i., but in this model, the upregulation was only partially dependent on IFN-γ and T cells, indicating that at least one other mediator was involved in the upregulation of SOCS following YF infection. We conclude that virus-induced inflammation of the CNS is associated with upregulation of SOCS1/3 mRNA expression in brain resident cells and that at least two distinctive pathways can lead to this upregulation.
  
  
  OBJECTIVE
  In the present report, we have studied the induction of SOCS1 and SOCS3 expression in the context of virus-induced CNS infection. We found that both a noncytolytic and a cytolytic virus induce marked upregulation of SOCS1 and -3 expression. Notably, the kinetics of the observed upregulation follows that of activity within proinflammatory signaling pathways and, interestingly, type II interferon (IFN), which is also a key inducer of inflammatory mediators, seems to be essential in initiating this counterinflammatory response. Another key observation is that not only cells of the immune system but also CNS resident cells are actively involved in both the pro- and the counterinflammatory immune circuits; thus, for example, astrocytes upregulate both C-X-C-motif chemokine 10 (CXCL10) and SOCS when exposed to type II IFN in vivo.
authors.affiliation: Department of International Health, Immunology, and Microbiology, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Maria Abildgaard
authors.initials: MA
authors.lastname: Steffensen
authors.affiliation: Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark.
authors.forename: Christina
authors.initials: C
authors.lastname: Fenger
authors.affiliation: Department of International Health, Immunology, and Microbiology, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Jeanette Erbo
authors.initials: JE
authors.lastname: Christensen
authors.affiliation: Department of International Health, Immunology, and Microbiology, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Carina Krogsgaard
authors.initials: CK
authors.lastname: Jørgensen
authors.affiliation: Department of International Health, Immunology, and Microbiology, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Bassi
authors.affiliation: Department of International Health, Immunology, and Microbiology, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Jan Pravsgaard
authors.initials: JP
authors.lastname: Christensen
authors.affiliation: Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark.
authors.forename: Bente
authors.initials: B
authors.lastname: Finsen
authors.affiliation: Department of International Health, Immunology, and Microbiology, University of Copenhagen, Copenhagen, Denmark athomsen@sund.ku.dk.
authors.forename: Allan Randrup
authors.initials: AR
authors.lastname: Thomsen
mesh_terms: D000818:Animals; D001117:Arenaviridae Infections / Q000276:immunology / Q000473:pathology* / Q000821:virology; D018792:Encephalitis, Viral / Q000276:immunology / Q000473:pathology* / Q000821:virology; D018177:Flavivirus Infections / Q000276:immunology / Q000473:pathology* / Q000821:virology; D020869:Gene Expression Profiling; D007371:Interferon-gamma / Q000276:immunology; D008216:Lymphocytic Choriomeningitis / Q000276:immunology*; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology*; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000032:analysis; D050826:Suppressor of Cytokine Signaling Proteins / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; D050826:Suppressor of Cytokine Signaling Proteins; D007371:Interferon-gamma