title: Bacillus cihuensis sp. nov., isolated from rhizosphere soil of a plant in the Cihu area of Taiwan.
abstract: A Gram-positive, moderately halotolerant, rod-shaped, spore forming bacterium, designated strain FJAT-14515(T) was isolated from a soil sample in Cihu area, Taoyuan County, Taiwan. The strain grew at 10-35 °C (optimum at 30 °C), pH 5.7-9.0 (optimum at pH 7.0) and at salinities of 0-5 % (w/v) NaCl (optimum at 1 % w/v). The diagnostic diamino acid of the peptidoglycan of the isolated strain was meso-diaminopimelic acid and major respiratory isoprenoid quinone was MK-7. Major cellular fatty acids were anteiso-C15:0 (40.6 %), iso-C15:0 (20.7 %) and the DNA G+C content of strain FJAT-14515(T) was 37.1 mol %. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FJAT-14515(T) belongs to the genus Bacillus, and was most closely related to the reference strains of Bacillus muralis DSM 16288(T) (97.6 %) and Bacillus simplex DSM 1321(T) (97.5 %). Levels of DNA-DNA relatedness between strain FJAT-14515(T) and the reference strains of B. muralis DSM 16288(T) and B. simplex DSM 1321(T) were 27.9 % ± 3.32 and 44.1 % ± 0.57, respectively. Therefore, on the basis of phenotypic, chemotaxonomic and genotypic properties, strain FJAT-14515(T) represents a novel species of the genus Bacillus, for which the name Bacillus cihuensis sp. nov. is proposed. The type strain is FJAT-14515(T) (=DSM 25969(T) = CGMCC 1.12697(T)).
authors.affiliation: Agricultural Bio-resource Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, Fujian, China, fzliubo@163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Guo-Hong
authors.initials: GH
authors.lastname: Liu
authors.forename: Cetin
authors.initials: C
authors.lastname: Sengonca
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.forename: Ming-Kuang
authors.initials: MK
authors.lastname: Wang
authors.forename: Jian-Yang
authors.initials: JY
authors.lastname: Tang
authors.forename: Mei-Chun
authors.initials: MC
authors.lastname: Chen
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D013624:Taiwan; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D003960:Diaminopimelic Acid