title: Neuroprotective effect of Buddleja cordata methanolic extract in the 1-methyl-4-phenylpyridinium Parkinson's disease rat model.
abstract: Parkinson's disease (PD) is a neurodegenerative disorder characterized by the irreversible loss of dopaminergic neurons in the nigrostriatal pathway with subsequent dopamine deficiency. Environmental causes have been proposed through molecules, such as 1-methyl-4-phenylpyridinium (MPP(+)), to induce oxidative stress. The methanolic extract of plants of the genus Buddleja has been reported to have in vitro and in vivo antioxidant properties to protect against neuronal death. In the present study, the neuroprotective effect of Buddleja cordata methanolic extract in the MPP(+) PD rat model was investigated. Animals were administered orally with 50 or 100 mg/kg of methanolic extract every 24 h for 14 days. Twenty hours later, rats were infused with an intrastriatal stereotaxic microinjection of 10 µg MPP(+) in 8 μl sterile saline solution. Six days later, the animals were treated with 1 mg/kg apomorphine to record ipsilateral rotations for 1 h. All the rats were killed by decapitation and the lesioned striatum was dissected for dopamine and lipid peroxidation quantifications. Both methanolic extract doses led to a significantly lower (P < 0.05) number of ipsilateral rotations (75-80 %). This behavioral protection was corroborated with 60 % level of dopamine preservation (P < 0.05) and 90 % decrease in the formation of lipidic fluorescent products in the striatum (P < 0.05). This study demonstrates the antioxidant and neuroprotective effect of Buddleja cordata methanolic extract in the MPP(+) PD rat model, possibly due to the involvement of phenylpropanoids.
authors.affiliation: Laboratorio de Neuroprotección, Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001 Col. Chamilpa, CP 62209, Cuernavaca, Morelos, México.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Pérez-Barrón
authors.forename: José Guillermo
authors.initials: JG
authors.lastname: Avila-Acevedo
authors.forename: Ana María
authors.initials: AM
authors.lastname: García-Bores
authors.forename: Sergio
authors.initials: S
authors.lastname: Montes
authors.forename: Sara
authors.initials: S
authors.lastname: García-Jiménez
authors.forename: Ismael
authors.initials: I
authors.lastname: León-Rivera
authors.forename: Moisés
authors.initials: M
authors.lastname: Rubio-Osornio
authors.forename: Antonio
authors.initials: A
authors.lastname: Monroy-Noyola
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D032861:Buddleja; D003342:Corpus Striatum / Q000737:chemistry; D004298:Dopamine / Q000032:analysis; D015227:Lipid Peroxidation / Q000187:drug effects; D020267:MPTP Poisoning / Q000188:drug therapy*; D008297:Male; D000432:Methanol; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D018696:Neuroprotective Agents; D010936:Plant Extracts; D004298:Dopamine; D000432:Methanol