title: [Identification of Scolopendra subspinipes mutilans and its adulterants using DNA barcode].
abstract: In this study, the COI barcode was used to identify the Scolopendra medicinal materials and its adulterants in order to provide a new method for the identification of Scolopendra. Genomic DNA was extracted from the experimental samples. The COI sequences were amplified and sequenced bi-directionally. Sequence alignment and NJ tree construction was carried out by MEGA6.0 software. The results showed that the COI sequences can be obtained from all experimental samples. The average inter-specific K2P distance of Scolopendra was 0.222 and the minimum inter-specific distance was 0.190. All the Scolopendra subspinipes mutilans medicinal samples clustered into a clade in the NJ tree and can be distinguished from its adulterants. In a conclusion, COI can be used to correctly identify Scolopendra medicinal materials, and it will be a potential DNA barcode for identifying other animal medicinal materials.
authors.forename: Hong-Yin
authors.initials: HY
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Jia
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.forename: Lin-Chun
authors.initials: LC
authors.lastname: Shi
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Jing-Yuan
authors.initials: JY
authors.lastname: Song
authors.forename: Hui
authors.initials: H
authors.lastname: Yao
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics*; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D004340:Drug Contamination / Q000517:prevention & control; D003576:Electron Transport Complex IV / Q000235:genetics*; D008516:Medicine, Chinese Traditional; D008969:Molecular Sequence Data; D010802:Phylogeny; D011786:Quality Control; D012605:Scorpions / Q000145:classification* / Q000201:enzymology / Q000235:genetics*
chemical_list: D060829:Arthropod Proteins; C038862:cytochrome o oxidase; D003576:Electron Transport Complex IV