title: Immobilization of pectinesterase in genipin-crosslinked chitosan membrane for low methoxyl pectin production.
abstract: In this study, an environmentally friendly method for preparation of chitosan crosslinked membranes with different genipin concentrations (0, 125, 250, and 500 mg/L) was developed. Genipin-crosslinked chitosan membranes were used for the immobilization of fungal pectinesterase (PE). PE was efficiently immobilized in chitosan membranes and used for modification of high-methoxylated pectins into low-methoxylated pectins. The charge density (ζ-potential), infrared spectroscopy (FTIR), and ion-exchange chromatography (IEC) revealed the modification of pectin for immobilized PE in genipin-crosslinked chitosan membrane. The ζ-potential results indicated a decrease of -5.2 mV in the pectin control solution, whereas using the PE immobilized in chitosan and genipin-chitosan membranes, the obtained values were -30.45 mV and -36.38 mV, respectively. Genipin-crosslinked chitosan membrane jointly with the immobilized PE represents an eco-friendly support to prepare tailor-made low-methoxylated lime pectin.
authors.affiliation: Centro de Investigación en Materiales Avanzados, S.C. (CIMAV), Unidad Monterrey, Alianza Norte 202, 66600, Apodaca, Nuevo León, México, adriana.rangel@cimav.edu.mx.
authors.forename: Adriana Marisol
authors.initials: AM
authors.lastname: Rangel-Rodríguez
authors.forename: Solans
authors.initials: S
authors.lastname: Conxita
authors.forename: Vílchez
authors.initials: V
authors.lastname: Susana
authors.forename: Sergio Gabriel
authors.initials: SG
authors.lastname: Flores-Gallardo
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Contreras-Esquivel
authors.forename: Liliana
authors.initials: L
authors.lastname: Licea-Jiménez
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry*; D039823:Iridoids / Q000737:chemistry*; D008567:Membranes, Artificial; D010368:Pectins / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D039823:Iridoids; D008567:Membranes, Artificial; D010368:Pectins; D048271:Chitosan; C007834:genipin; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase