title: Statistical experimental design to assess the influence of enzymes of nematophagous fungi versus helminths.
abstract: The present work used Plackett-Burman experimental design to assess the influence of enzymes of nematophagous fungi versus Strongyloides westeri and trichostrongylides larvae and Platynosomum fastosum eggs. The variables studied in the Plackett-Burman design were the proteases and chitinases of AC001 or VC4 as destructive agents of S. westeri and trichostrongylides larvae, and P. fastosum eggs. All tested enzymes had a significant effect (P < 0.05) on the destruction of S. westeri larvae. Furthermore, only VC4 and AC001 proteases showed a significant effect (P < 0.05) on the destruction of trichostrongylides larvae. On the other hand, chitinases of VC4 showed the highest significance (P < 0.05) on the destruction of P. fastosum eggs. It is proposed that statistical planning for the use of enzymes derived from nematophagous fungi is a viable way to elucidate some questions about their mechanism of action.
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG 36570-000, Brazil; Universidade Vila Velha, Espírito Santo, Brazil. Electronic address: fabioribeirobraga@hotmail.com.
authors.forename: Fabio Ribeiro
authors.initials: FR
authors.lastname: Braga
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG 36570-000, Brazil.
authors.forename: Filippe Elias
authors.initials: FE
authors.lastname: de Freitas Soares
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG 36570-000, Brazil.
authors.forename: Juliana Milani
authors.initials: JM
authors.lastname: Araujo
authors.affiliation: Universidade Vila Velha, Espírito Santo, Brazil.
authors.forename: Leandro Abreu
authors.initials: LA
authors.lastname: da Fonseca
authors.affiliation: Universidade Vila Velha, Espírito Santo, Brazil.
authors.forename: Emy
authors.initials: E
authors.lastname: Hiura
authors.affiliation: Universidade Vila Velha, Espírito Santo, Brazil.
authors.forename: Maylla
authors.initials: M
authors.lastname: Garschagen Gava
authors.affiliation: Universidade Vila Velha, Espírito Santo, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Toledo Vieira
authors.affiliation: Universidade Vila Velha, Espírito Santo, Brazil.
authors.forename: Jeanne Saraiva
authors.initials: JS
authors.lastname: da Paz
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG 36570-000, Brazil.
authors.forename: Lorendane Milena
authors.initials: LM
authors.lastname: de Carvalho
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG 36570-000, Brazil.
authors.forename: João Victor
authors.initials: JV
authors.lastname: Faccini
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG 36570-000, Brazil.
authors.forename: José Humberto
authors.initials: JH
authors.lastname: de Queiroz
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG 36570-000, Brazil.
authors.forename: Jackson Victor
authors.initials: JV
authors.lastname: Araújo
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000201:enzymology*; D002688:Chitinases / Q000378:metabolism; D004012:Dicrocoeliidae / Q000254:growth & development / Q000502:physiology*; D015801:Helminth Proteins / Q000378:metabolism*; D006999:Hypocreales / Q000201:enzymology; D007814:Larva / Q000502:physiology; D010063:Ovum / Q000502:physiology; D010447:Peptide Hydrolases / Q000378:metabolism; D010572:Pest Control, Biological; D013321:Strongyloides / Q000254:growth & development / Q000502:physiology*; D014251:Trichostrongyloidea / Q000254:growth & development / Q000502:physiology*
chemical_list: D015801:Helminth Proteins; D002688:Chitinases; D010447:Peptide Hydrolases
keywords: Biological control; Fungal enzymes; Helminths; Plackett–Burman