title: IL-4/CCL22/CCR4 axis controls regulatory T-cell migration that suppresses inflammatory bone loss in murine experimental periodontitis.
abstract: Inflammatory bone resorption is a hallmark of periodontitis, and Tregs and Th2 cells are independently associated with disease progression attenuation. In this study, we employed an infection-triggered inflammatory osteolysis model to investigate the mechanisms underlying Treg and Th2 cell migration and the impact on disease outcome. Aggregatibacter actinomycetemcomitans-infected C57Bl/6 (wild-type [WT]) mice develop an intense inflammatory reaction and alveolar bone resorption, and Treg and Th2 cell migration is temporally associated with disease progression attenuation. Tregs extracted from the lesions preferentially express CCR4 and CCR8, whereas Th2 cells express CCR3, CCR4, and CCR8. The absence of CCR5 and CCR8 did not significantly impact the migration of Tregs and Th2 cells or affect the disease outcome. CCR4KO mice presented a minor reduction in Th2 cells in parallel with major impairment of Treg migration, which was associated with increased inflammatory bone loss and higher proinflammatory and osteoclastogenic cytokine levels. The blockade of the CCR4 ligand CCL22 in WT mice resulted in an increased inflammatory bone loss phenotype similar to that in the CCR4KO strain. Adoptive transfer of CCR4(+) Tregs to the CCR4KO strain revert the increased disease phenotype to WT mice-like levels; also, the in situ production of CCL22 in the lesions is mandatory for Tregs migration and the consequent bone loss arrest. The local release of exogenous CCL22 provided by poly(lactic-co-glycolic acid) (PLGA) microparticles promotes migration of Tregs and disease arrest in the absence of endogenous CCL22 in the IL-4KO strain, characterized by the lack of endogenous CCL22 production, defective migration of Tregs, and exacerbated bone loss. In summary, our results show that the IL-4/CCL22/CCR4 axis is involved in the migration of Tregs to osteolytic lesion sites, and attenuates development of lesions by inhibiting inflammatory migration and the production of proinflammatory and osteoclastogenic mediators.
authors.affiliation: Department of Biological Sciences, School of Dentistry of Bauru, Sao Paulo University (FOB/USP), Bauru, SP, Brazil.
authors.forename: Ana Claudia
authors.initials: AC
authors.lastname: Araujo-Pires
authors.forename: Andreia Espindola
authors.initials: AE
authors.lastname: Vieira
authors.forename: Carolina Favaro
authors.initials: CF
authors.lastname: Francisconi
authors.forename: Claudia Cristina
authors.initials: CC
authors.lastname: Biguetti
authors.forename: Andrew
authors.initials: A
authors.lastname: Glowacki
authors.forename: Sayuri
authors.initials: S
authors.lastname: Yoshizawa
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Campanelli
authors.forename: Ana Paula Favaro
authors.initials: AP
authors.lastname: Trombone
authors.forename: Charles S
authors.initials: CS
authors.lastname: Sfeir
authors.forename: Steven R
authors.initials: SR
authors.lastname: Little
authors.forename: Gustavo Pompermaier
authors.initials: GP
authors.lastname: Garlet
mesh_terms: D000818:Animals; D054422:Chemokine CCL22 / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010000:Osteitis / Q000378:metabolism / Q000473:pathology*; D010024:Osteoporosis / Q000473:pathology*; D010518:Periodontitis / Q000378:metabolism / Q000473:pathology*; D050378:T-Lymphocytes, Regulatory / Q000473:pathology*
chemical_list: C515483:Ccl22 protein, mouse; D054422:Chemokine CCL22; D015847:Interleukin-4
keywords: BONE RESORPTION; CHEMOKINES; CYTOKINES; OSTEOIMMUNOLOGY; PERIODONTAL DISEASE