title: Soy isoflavones have antimutagenic activity on DNA damage induced by the antileishmanial Glucantime (meglumine antimoniate).
abstract: Isoflavones are phytoestrogens reported to be potent antioxidant agents. In contrast, the antileishmanial meglumine antimoniate has mutagenic activities. This study evaluated the ability of soy isoflavones to reduce DNA damage induced by meglumine antimoniate. Antimutagenic effects (by micronucleus test) were tested using Swiss mice divided into seven groups treated with meglumine antimoniate (425 mg/kg bw pentavalent antimony); cyclophosphamide (50 mg/kg bw); water (negative control); single isoflavones dose (1.6 mg/kg bw), and three groups received one dose of isoflavones via gavage (0.4 mg/kg bw, 0.8 mg/kg bw or 1.6 mg/kg bw) plus meglumine antimoniate via intraperitoneal, simultaneously. To evaluate antigenotoxicity (by Comet assay), each group with 10 animals received the above-mentioned control doses; single dose of isoflavones 0.8 mg/kg bw, and three groups received isoflavones (0.8 mg/kg bw) by gavage along with intraperitoneal meglumine antimoniate, which were treated with isoflavones 24 h before or after receiving meglumine antimoniate (pre-treatment and post-treatment, respectively) or simultaneously. Cells were harvested 24 h after the treatment, and the data were evaluated by ANOVA followed by Tukey's test (p < 0.05). The data from the simultaneous treatment by micronucleus test revealed that isoflavones (0.4 and 0.8 mg/kg) were able to reverse the mutagenic effect of Glucantime. Moreover, all regimes of the treatment with 0.8 mg/kg bw dose were able to reduce the genotoxicity caused by meglumine antimoniate. It is suggested that the protective effect of isoflavones against DNA damage is related to their ability to reduce oxidative stress caused by the trivalent Sb(III) metabolite of meglumine antimoniate.
authors.affiliation: Laboratory of Genetics and Molecular Biology, Department of Biology, Federal University of Maranhão, Cidade Universitária do Bacanga , São Luís, Maranhão , Brazil.
authors.forename: Ludymila Furtado
authors.initials: LF
authors.lastname: Cantanhêde
authors.forename: Laís Pinheiro
authors.initials: LP
authors.lastname: Almeida
authors.forename: Rossy-Eric Pereira
authors.initials: RE
authors.lastname: Soares
authors.forename: Patrícia Valéria Gomes
authors.initials: PV
authors.lastname: Castelo Branco
authors.forename: Silma Regina Ferreira
authors.initials: SR
authors.lastname: Pereira
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D000981:Antiprotozoal Agents / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D007529:Isoflavones / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D008536:Meglumine / Q000633:toxicity*; D000077485:Meglumine Antimoniate; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced / Q000187:drug effects; D015162:Micronucleus Tests; D009154:Mutation / Q000187:drug effects*; D009942:Organometallic Compounds / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D013025:Glycine max / Q000737:chemistry
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D000981:Antiprotozoal Agents; D007529:Isoflavones; D009942:Organometallic Compounds; D008536:Meglumine; D000077485:Meglumine Antimoniate
keywords: Comet assay; mice; micronucleus test