title: Validation and application of an rapid HPLC-MS method for the determination of salvianic acid A in human plasma.
abstract: A rapid liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS-MS) method was developed and validated for the determination of salvianic acid A in plasma of Chinese healthy subjects after oral administration of Qishenyiqi dropping pills. After liquid-liquid extraction with ethyl acetate, salvianic acid A was chromatographed on a Agilent Zorbax XDB-C18 column using a gradient mobile phase consisting of water (0.1% formic acid)-acetonitrile (0.1% formic acid) at a flow rate of 0.45 mL/min. The detection was performed in multiple reaction monitoring mode, using the transitions of m/z 196.9→134.8 and m/z 320.9→151.9 for salvianic acid A and chloroamphenicol, respectively. The method was linear over the range of 0.50-500 ng/mL using only 100 μL of plasma and the lower limit of quantification was 0.50 ng/mL. Intra-day and inter-day precisions (in terms of % RSD) were all <15% and the accuracies (in terms of % RE) were within the range of±15%, and recoveries were between 85.0 and 115%. The validated method was successfully applied to pharmacokinetic study of Qishenyiqi dropping pills in Chinese healthy subjects. After oral administration, Tmax and Cmax values were 1.33 ± 0.52 h and 21.1 ± 3.92 ng/mL, respectively. Plasma concentrations declined with t1/2Z of 1.76 ± 0.33 h.
authors.affiliation: Traditional Chinese Medicine Department of Internal Medicine of Cardiovascular Center, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China autumnfeng@126.com.
authors.forename: Limin
authors.initials: L
authors.lastname: Feng
authors.affiliation: Faculty of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Fu
authors.affiliation: Traditional Chinese Medicine Department of Internal Medicine of Cardiovascular Center, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China.
authors.forename: Wuxun
authors.initials: W
authors.lastname: Du
authors.affiliation: Traditional Chinese Medicine Department of Internal Medicine of Cardiovascular Center, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China.
authors.forename: Baohe
authors.initials: B
authors.lastname: Wang
authors.affiliation: Tianjin Public Security Hospital, Tianjin 300042, China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Li
authors.affiliation: Traditional Chinese Medicine Department of Internal Medicine of Cardiovascular Center, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China.
authors.forename: Mingdan
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Traditional Chinese Medicine Department of Internal Medicine of Cardiovascular Center, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China.
authors.forename: Changyu
authors.initials: C
authors.lastname: Liu
authors.affiliation: Traditional Chinese Medicine Department of Internal Medicine of Cardiovascular Center, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000284:Administration, Oral; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics; D006801:Humans; D007773:Lactates / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D007773:Lactates; C035055:3,4-dihydroxyphenyllactic acid