title: Identification of gene expression profiles in the actinomycete Gordonia neofelifaecis grown with different steroids.
abstract: Gordonia neofelifaecis NRRL B-59395 was initially isolated from the fresh feces of a clouded leopard based on its ability to degrade cholesterol. The transcriptome profiles of G. neofelifaecis NRRL B-59395 grown with cholesterol, androstenedione (AD), and pyruvic acid were compared by RNA-Seq. The sterol catabolic genes are highly conserved in G. neofelifaecis, Rhodococcus jostii RHA1, and Mycobacterium tuberculosis. The RNA-Seq results indicated that the genes involved in the sterol side chain cleavage were exclusively induced by cholesterol, while the genes involved in the degradation of rings A/B and C/D were up-regulated by both cholesterol and AD. It appears that the induction mechanisms for the genes responsible for side chain cleavage and those for degradation of rings are different. There are approximately 21 genes encoding transporter proteins that are differentially expressed in cholesterol or AD compared with pyruvic acid. The genes camABCD and camM encode two systems that take up cholate, and they have been shown to be cholesterol- and AD-inducible. The potential biological functions of other differentially expressed genes are also discussed. These results will promote the functional characterization of the sterol catabolic genes and also provide important clues in understanding the mechanisms of their gene expression, and they may help us understand the mechanism underlying microbial cholesterol catabolism.
authors.affiliation: College of life Sciences, Sichuan Normal University, Chengdu 610101, P.R. China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Li
authors.forename: Fanglan
authors.initials: F
authors.lastname: Ge
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yao
authors.initials: Y
authors.lastname: Ren
authors.forename: Jiadai
authors.initials: J
authors.lastname: Yuan
authors.forename: Junzhong
authors.initials: J
authors.lastname: He
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Guiying
authors.initials: G
authors.lastname: Chen
authors.forename: Guangxiang
authors.initials: G
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhuang
authors.forename: Li
authors.initials: L
authors.lastname: Xu
mesh_terms: D000735:Androstenedione / Q000378:metabolism / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D033601:Gordonia Bacterium / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D009876:Operon; D019289:Pyruvic Acid / Q000378:metabolism / Q000494:pharmacology; D013256:Steroids / Q000378:metabolism* / Q000494:pharmacology; D059467:Transcriptome
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D013256:Steroids; D000735:Androstenedione; D019289:Pyruvic Acid; D002784:Cholesterol
keywords: Gordonia neofelifaecis; RNA-Seq; RNA-seq; androstenedione; androstènedione; cholesterol; cholestérol; differentially expressed gene; expression génique différentielle