title: Cerium oxide-deposited mesoporous silica nanoparticles for the determination of carcinoembryonic antigen in serum using inductively coupled plasma-mass spectrometry.
abstract: CeO2-deposited mesoporous silica nanoparticles were synthesized as a probe to determine carcinoembryonic antigen (CEA) in serum by inductively coupled plasma-mass spectrometry (ICP-MS). The prepared mesoporous nanoparticles were modified and tagged to the target for sandwich-type immunoassay. Fe3O4 magnetic nanoparticles (MNPs) were also synthesized and immobilized with antibody to extract the target biomarker. The calibration curve of the synthesized CeO2-deposited silica nanoparticles, which was plotted by the signal ratio of (140)Ce/(57)Fe measured by ICP-MS vs. the concentration of CEA, showed excellent linearity and sensitivity owing to the signal amplification and low spectral interference. Under optimal conditions, the sandwich-type analytical method was applied to determine CEA in serum spiked in the range of 0.001-5 ng mL(-1) and showed a limit of detection of 0.36 ng mL(-1). Since the deposited CeO2 in the mesoporous silica layer can be substituted by other metal compounds, various kinds of metal-deposited nanoparticles can be prepared as probe materials for multiplex detection in bioanalysis.
authors.affiliation: Department of Chemistry, NSBI, Dankook University, 126 Jukjeon-dong, Suji-gu, Yongin-si, Gyeonggi-do 448-701, South Korea.
authors.forename: H W
authors.initials: HW
authors.lastname: Choi
authors.affiliation: Department of Chemistry, Sogang University, Shinsu-dong, Mapo-gu, Seoul, South Korea.
authors.forename: K H
authors.initials: KH
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Sogang University, Shinsu-dong, Mapo-gu, Seoul, South Korea.
authors.forename: N H
authors.initials: NH
authors.lastname: Hur
authors.affiliation: Department of Chemistry, NSBI, Dankook University, 126 Jukjeon-dong, Suji-gu, Yongin-si, Gyeonggi-do 448-701, South Korea. Electronic address: plasma@dankook.ac.kr.
authors.forename: H B
authors.initials: HB
authors.lastname: Lim
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry*; D002272:Carcinoembryonic Antigen / Q000097:blood*; D002563:Cerium / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D013058:Mass Spectrometry / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D002272:Carcinoembryonic Antigen; D058185:Magnetite Nanoparticles; D002563:Cerium; C030583:ceric oxide; D012822:Silicon Dioxide