title: Long-term vitamin D3 supplementation does not prevent colonic inflammation or modulate bone health in IL-10 knockout mice at young adulthood.
abstract: Inflammatory bowel disease (IBD) is an idiopathic disease that can impair bone metabolism. Low vitamin D status has been implicated in its progress. This study used interleukin (IL)-10 knockout (KO) mice, that develop an intestinal inflammation when housed in a non-sterile environment, to determine if supplementation with vitamin D3 throughout life could mitigate inflammation and attenuate the lower bone mineral content (BMC) and density (BMD), and bone strength. Female IL-10 KO mice were randomized 25 or 5000 IU vitamin D3/kg diet throughout pregnancy and lactation. At weaning, offspring received the same or opposite diet as their mother until age three months. Body weight growth was similar among groups within a sex. At three months of age, there were no differences in inflammation and gene expression in the colon of offspring. Male offspring exposed to continuous 25 IU vitamin D3/kg diet had lower (p < 0.001) colonic VDR expression and those exposed only to low vitamin D3 until weaning had higher serum IL-6. There were no differences in femur or vertebral BMC, BMD or bone strength. In summary, long-term exposure to vitamin D3 did not attenuate intestinal inflammation or preserve bone mineral or bone strength. Thus, supplementation with vitamin D3 does not exert anti-inflammatory effects in this mouse model that mimics human inflammatory bowel disease.
authors.affiliation: Faculty of Medicine, University of Toronto, Toronto, ON M5S 3E2, Canada. andrea.glenn@utoronto.ca.
authors.forename: Andrea J
authors.initials: AJ
authors.lastname: Glenn
authors.affiliation: Faculty of Medicine, University of Toronto, Toronto, ON M5S 3E2, Canada. kristina.fielding@utoronto.ca.
authors.forename: Kristina A
authors.initials: KA
authors.lastname: Fielding
authors.affiliation: Faculty of Medicine, University of Toronto, Toronto, ON M5S 3E2, Canada. jimmy.chen@utoronto.ca.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Chen
authors.affiliation: Faculty of Medicine, University of Toronto, Toronto, ON M5S 3E2, Canada. elena.comelli@utoronto.ca.
authors.forename: Elena M
authors.initials: EM
authors.lastname: Comelli
authors.affiliation: Faculty of Medicine, University of Toronto, Toronto, ON M5S 3E2, Canada. wward@brocku.ca.
authors.forename: Wendy E
authors.initials: WE
authors.lastname: Ward
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects; D050071:Bone Density Conservation Agents / Q000494:pharmacology / Q000627:therapeutic use; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism; D003106:Colon / Q000187:drug effects* / Q000473:pathology; D019587:Dietary Supplements; D004195:Disease Models, Animal; D005260:Female; D005269:Femur / Q000187:drug effects / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism* / Q000517:prevention & control; D015212:Inflammatory Bowel Diseases / Q000188:drug therapy / Q000378:metabolism*; D016753:Interleukin-10 / Q000378:metabolism*; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D018345:Mice, Knockout; D018167:Receptors, Calcitriol / Q000378:metabolism; D014807:Vitamin D / Q000494:pharmacology* / Q000627:therapeutic use; D014815:Vitamins / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D050071:Bone Density Conservation Agents; D015850:Interleukin-6; D018167:Receptors, Calcitriol; D014815:Vitamins; D016753:Interleukin-10; D014807:Vitamin D