title: Interferon regulatory factor 8 expressed in microglia contributes to tactile allodynia induced by repeated cold stress in rodents.
abstract: We investigated the role of interferon regulatory factor 8 (IRF8) in a model of chronic pain in which repeated cold stress (RCS) exposure produces tactile allodynia. RCS exposure produced a decrease in paw withdrawal threshold (PWT) to mechanical stimulation. Spinal microglia of RCS-exposed mice were morphologically activated. Expression of IRF8 was significantly increased in the spinal cord of RCS-exposed mice and was localized in microglia. IRF8-knockout mice failed to show the RCS-induced decrease in PWT. Thus, RCS exposure activates spinal microglia and upregulation of IRF8 in these cells is involved in the development of tactile allodynia after RCS exposure.
authors.affiliation: Department of Molecular and System Pharmacology, Graduate School of Pharmaceutical Sciences, Kyushu University, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Akagi
authors.forename: Yuta
authors.initials: Y
authors.lastname: Matsumura
authors.forename: Masaya
authors.initials: M
authors.lastname: Yasui
authors.forename: Emiko
authors.initials: E
authors.lastname: Minami
authors.forename: Hidemasa
authors.initials: H
authors.lastname: Inoue
authors.forename: Takahiro
authors.initials: T
authors.lastname: Masuda
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Tozaki-Saitoh
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Tamura
authors.forename: Kazue
authors.initials: K
authors.lastname: Mizumura
authors.forename: Makoto
authors.initials: M
authors.lastname: Tsuda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyama
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Inoue
mesh_terms: D000818:Animals; D002908:Chronic Disease; D058639:Cold-Shock Response / Q000502:physiology*; D004056:Differential Threshold; D004195:Disease Models, Animal; D005121:Extremities / Q000503:physiopathology; D015870:Gene Expression / Q000502:physiology*; D006930:Hyperalgesia / Q000209:etiology*; D050835:Interferon Regulatory Factors / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D008297:Male; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism; D015854:Up-Regulation; D000099185:Interferon Regulatory Factor-8
chemical_list: D050835:Interferon Regulatory Factors; D000099185:Interferon Regulatory Factor-8