title: Supported core-shell nanobiosensors for quantitative fluorescence imaging of extracellular pH.
abstract: Covalent "click" cycloaddition was used to functionalize silica substrates with pH-sensitive nanoparticles, thus producing uniform and highly luminescent analytical devices usable in both commercial fluorimeters and fluorescence microscopes. Quantitative and spatially-resolved extracellular pH measurements were successfully achieved on live cardiac fibroblasts with these novel ion-sensitive surfaces.
authors.affiliation: Département de chimie, Faculté de Sciences et Génie, Université Laval, Québec (QC), Canada G1V 0A6. denis.boudreau@chm.ulaval.ca.
authors.forename: Jérémie
authors.initials: J
authors.lastname: Asselin
authors.forename: Carl
authors.initials: C
authors.lastname: Roy
authors.forename: Denis
authors.initials: D
authors.lastname: Boudreau
authors.forename: Younès
authors.initials: Y
authors.lastname: Messaddeq
authors.forename: Rihab
authors.initials: R
authors.lastname: Bouchareb
authors.forename: Patrick
authors.initials: P
authors.lastname: Mathieu
mesh_terms: D057930:Click Chemistry; D005110:Extracellular Space / Q000737:chemistry*; D005347:Fibroblasts; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence; D009206:Myocardium / Q000166:cytology; D053758:Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D012834:Silver; D012822:Silicon Dioxide; D016650:Fluorescein-5-isothiocyanate