title: Analysis of mouse liver metabolites by GC × GC-TOF MS.
abstract: Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOF MS) is a powerful analytical platform for analysis of metabolites in mouse liver. Metabolites are extracted using a solvent mixture of methanol/water (4:1, v/v). The metabolite extracts are analyzed on GC × GC-TOF MS after derivatization. The experimental data are further processed for metabolite identification and quantification. A three-tier method is used for high-accuracy metabolite identification, while metabolite quantification is performed to recognize metabolites with significant abundance differences between sample groups.
authors.affiliation: Department of Chemistry, University of Louisville, 2320 South Brook Road, Louisville, KY, 40292, USA.
authors.forename: Xue
authors.initials: X
authors.lastname: Shi
authors.forename: Xinmin
authors.initials: X
authors.lastname: Yin
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D008099:Liver / Q000378:metabolism*; D055442:Metabolome; D055432:Metabolomics / Q000295:instrumentation / Q000379:methods*; D051379:Mice