title: Sequencing pools of individuals - mining genome-wide polymorphism data without big funding.
abstract: The analysis of polymorphism data is becoming increasingly important as a complementary tool to classical genetic analyses. Nevertheless, despite plunging sequencing costs, genomic sequencing of individuals at the population scale is still restricted to a few model species. Whole-genome sequencing of pools of individuals (Pool-seq) provides a cost-effective alternative to sequencing individuals separately. With the availability of custom-tailored software tools, Pool-seq is being increasingly used for population genomic research on both model and non-model organisms. In this Review, we not only demonstrate the breadth of questions that are being addressed by Pool-seq but also discuss its limitations and provide guidelines for users.
authors.affiliation: Institut für Populationsgenetik, Vetmeduni Vienna, Veterinärplatz 1, 1210 Vienna, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Schlötterer
authors.affiliation: 1] Institut für Populationsgenetik, Vetmeduni Vienna, Veterinärplatz 1, 1210 Vienna, Austria. [2] Vienna Graduate School of Population Genetics.
authors.forename: Raymond
authors.initials: R
authors.lastname: Tobler
authors.affiliation: Institut für Populationsgenetik, Vetmeduni Vienna, Veterinärplatz 1, 1210 Vienna, Austria.
authors.forename: Robert
authors.initials: R
authors.lastname: Kofler
authors.affiliation: Institut für Populationsgenetik, Vetmeduni Vienna, Veterinärplatz 1, 1210 Vienna, Austria.
authors.forename: Viola
authors.initials: V
authors.lastname: Nolte
mesh_terms: D057225:Data Mining; D015894:Genome, Human / Q000235:genetics*; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing / Q000191:economics / Q000379:methods*; D006801:Humans; D056186:Metagenomics / Q000191:economics / Q000379:methods*; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012984:Software