title: Development of a (1)H NMR structural-reporter-group concept for the analysis of prebiotic galacto-oligosaccharides of the [β-d-Galp-(1→x)]n-d-Glcp type.
abstract: Some β-galactosidases (EC 3.2.1.23) are capable of producing mixtures of linear and branched galacto-oligosaccharides (GOS) with various types of glycosidic linkages [degree of polymerization (DP) 2-8; mainly GalnGlc] when incubated under specific conditions with lactose. These products are generally applied in infant formula. However, for most galacto-oligosaccharide products only major components (low DP) or linkage patterns have been described. To build up a library of (1)H and (13)C NMR data, a detailed NMR study on commercially available GOS di- and trisaccharides, and some larger GOS oligosaccharides was carried out. Based on the fully assigned (1)H and (13)C chemical shifts of these model compounds, a (1)H NMR structural-reporter-group concept was formulated to function as a tool in the structural analysis of single GOS components and GOS mixtures.
authors.affiliation: Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, NL-9747 AG Groningen, The Netherlands.
authors.forename: Sander S
authors.initials: SS
authors.lastname: van Leeuwen
authors.affiliation: FrieslandCampina, Stationsplein 4, NL-3818 LE Amersfoort, The Netherlands.
authors.forename: Bas J H
authors.initials: BJH
authors.lastname: Kuipers
authors.affiliation: Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, NL-9747 AG Groningen, The Netherlands. Electronic address: l.dijkhuizen@rug.nl.
authors.forename: Lubbert
authors.initials: L
authors.lastname: Dijkhuizen
authors.affiliation: Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, NL-9747 AG Groningen, The Netherlands.
authors.forename: Johannis P
authors.initials: JP
authors.lastname: Kamerling
mesh_terms: D002250:Carbon Radioisotopes / Q000737:chemistry; D005690:Galactose / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000737:chemistry*; D056692:Prebiotics; D001616:beta-Galactosidase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D002250:Carbon Radioisotopes; D009844:Oligosaccharides; D056692:Prebiotics; D006859:Hydrogen; D001616:beta-Galactosidase; D005690:Galactose
keywords: Galacto-oligosaccharides; NMR spectroscopy; Prebiotics; Structural-reporter-group concept