title: Headspace SPME-GC-MS metabolomics analysis of urinary volatile organic compounds (VOCs).
abstract: In this chapter, we describe the procedure for metabolic profiling of hundreds of urinary volatile organic compounds (VOCs) using headspace solid-phase microextraction (SPME) coupled to gas chromatography-mass spectrometry (GC-MS). SPME is a solvent-free technology that is reproducible, fast, cost effective, and versatile in extracting small molecular weight organic compounds (metabolites) from biofluids using a fiber that is coated with an extracting phase. After extraction, the SPME fiber can be directly injected to the GC-MS, where the extracted metabolites will desorb thermally from the fiber, elute along a GC column, and finally enter into the MS for detection. The analysis of urine samples is presented using this approach.
authors.affiliation: Department of Neurosurgery, Nutrition Research Institute, Chapel Hill School of Public Health, Brigham and Women's Hospital, Boston, Massachusetts, USA, szhang17@partners.org.
authors.forename: Shucha
authors.initials: S
authors.lastname: Zhang
authors.forename: Daniel
authors.initials: D
authors.lastname: Raftery
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D055432:Metabolomics / Q000379:methods*; D052617:Solid Phase Microextraction / Q000379:methods*; D016482:Urinalysis / Q000379:methods; D055549:Volatile Organic Compounds / Q000302:isolation & purification / Q000378:metabolism* / Q000652:urine*
chemical_list: D055549:Volatile Organic Compounds