title: DNA-binding and cytotoxic efficacy studies of organorhenium pentylcarbonate compounds.
abstract: Seven organorhenium pentylcarbonate compounds (PC1-PC7) have been synthesized. DNA-binding studies of the PC-series compounds using electronic spectroscopy and gel electrophoresis suggest that the compounds presumably bind to DNA in an intercalative mode. The intrinsic binding constants for PC4, PC6, and PC7 were found to be 1.6 × 10(4), 3.9 × 10(4), and 4.2 × 10(4) M(-1), respectively. The X-ray structure determinations and density functional theory calculations indicate that the polypyridyl ligands in the compounds are nearly planar facilitating DNA binding through an intercalation mechanism. Cytotoxicity studies of 10 µM pentylcarbonate compounds against HTB-12 human astrocytoma brain cancer cells were studied for 48 h. It was observed that each of the pentylcarbonate compounds is active against the cancer cells. However, under analogous conditions, CRL-2005 rat astrocyte normal brain cells are not affected significantly.
authors.affiliation: Department of Biology, Elizabeth City State University, Elizabeth City, NC, 27909, USA.
authors.forename: Jewel
authors.initials: J
authors.lastname: Medley
authors.forename: Gloria
authors.initials: G
authors.lastname: Payne
authors.forename: Hirendra N
authors.initials: HN
authors.lastname: Banerjee
authors.forename: Dipak
authors.initials: D
authors.lastname: Giri
authors.forename: Angela
authors.initials: A
authors.lastname: Winstead
authors.forename: James M
authors.initials: JM
authors.lastname: Wachira
authors.forename: Jeanette A
authors.initials: JA
authors.lastname: Krause
authors.forename: Roosevelt
authors.initials: R
authors.lastname: Shaw
authors.forename: Saroj K
authors.initials: SK
authors.lastname: Pramanik
authors.forename: Santosh K
authors.initials: SK
authors.lastname: Mandal
mesh_terms: D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D018360:Crystallography, X-Ray; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007364:Intercalating Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D012428:Ruthenium / Q000737:chemistry*; D013053:Spectrophotometry
chemical_list: D007364:Intercalating Agents; D009942:Organometallic Compounds; D012428:Ruthenium; D004247:DNA