title: ICAM-1 as a molecular target for triple negative breast cancer.
abstract: Triple negative breast cancers (TNBCs) have a high mortality rate owing to aggressive proliferation and metastasis and a lack of effective therapeutic options. Herein, we describe the overexpression of intercellular adhesion molecule-1 (ICAM-1) in human TNBC cell lines and tissues, and demonstrate that ICAM-1 is a potential molecular target and biomarker for TNBC therapy and diagnosis. We synthesized ICAM-1 antibody-conjugated iron oxide nanoparticles (ICAM-IONPs) as a magnetic resonance imaging (MRI) probe to evaluate tumor targeting. Quantitative analysis of ICAM-1 surface expression predicted the targeting capability of ICAM-IONPs to TNBC cells. MRI of the TNBC xenograft tumor after systemic administration of ICAM-IONPs, coupled with iron quantification and histology, demonstrated a significant and sustained MRI contrast enhancement and probe accumulation in tumors with ICAM-1 overexpression relative to control. Identification of ICAM-1 as a TNBC target and biomarker may lead to the development of a new strategy and platform for addressing a critical gap in TNBC patient care.
authors.affiliation: Department of Biomedical Engineering, The City College of New York, New York, NY 10031; Vascular Biology Program, Boston Children's Hospital, Boston, MA 02115; Department of Surgery, Harvard Medical School, Boston, MA 02115;
authors.forename: Peng
authors.initials: P
authors.lastname: Guo
authors.affiliation: Department of Radiology and Imaging Sciences and Center for Systems Imaging, Emory University School of Medicine, Atlanta, GA 30322;
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Radiology and Imaging Sciences and Center for Systems Imaging, Emory University School of Medicine, Atlanta, GA 30322;
authors.forename: Liya
authors.initials: L
authors.lastname: Wang
authors.affiliation: Vascular Biology Program, Boston Children's Hospital, Boston, MA 02115; Department of Surgery, Harvard Medical School, Boston, MA 02115;
authors.forename: Di
authors.initials: D
authors.lastname: Jia
authors.affiliation: Vascular Biology Program, Boston Children's Hospital, Boston, MA 02115; Department of Surgery, Harvard Medical School, Boston, MA 02115;
authors.forename: Jiang
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115; and.
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Dillon
authors.affiliation: Department of Anesthesia, Boston Children's Hospital, Boston, MA 02115.
authors.forename: David
authors.initials: D
authors.lastname: Zurakowski
authors.affiliation: Department of Radiology and Imaging Sciences and Center for Systems Imaging, Emory University School of Medicine, Atlanta, GA 30322;
authors.forename: Hui
authors.initials: H
authors.lastname: Mao
authors.affiliation: Vascular Biology Program, Boston Children's Hospital, Boston, MA 02115; Department of Surgery, Harvard Medical School, Boston, MA 02115;
authors.forename: Marsha A
authors.initials: MA
authors.lastname: Moses
authors.affiliation: Department of Biomedical Engineering, The City College of New York, New York, NY 10031; Vascular Biology Program, Boston Children's Hospital, Boston, MA 02115; Department of Surgery, Harvard Medical School, Boston, MA 02115; dauguste@ccny.cuny.edu.
authors.forename: Debra T
authors.initials: DT
authors.lastname: Auguste
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D045744:Cell Line, Tumor; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008279:Magnetic Resonance Imaging; D051379:Mice; D058990:Molecular Targeted Therapy; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D064726:Triple Negative Breast Neoplasms / Q000378:metabolism* / Q000628:therapy*; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor; D005290:Ferric Compounds; D018799:Intercellular Adhesion Molecule-1; C000499:ferric oxide