title: Research on effect of minor bupleurum decoction of proliferation and apoptosis of esophageal cancer cell strain eca-109 cell.
abstract: The research protocol is MTT (Methyl Thiazolyl Tetrazolium) method, Hoechst33342 staining method and flow cytometry detection to observe the effect of minor bupleurum decoction on proliferation inhibition and apoptosis-inducing of esophageal cancer cell strain Eca-109 cell and its purpose is to discuss the effect. The result of MTT method shows that minor buplerum decoction can obviously inhibit proliferation of esophageal cancer cell strain Eca-109 cell. Apoptosis number of esophageal cancer cell increased with the increase of concentration of tetrandrine by the Hoechst 35528 staining experiment of cancer cell in three different concentrations. Flow cytometry detection result showed that cells in cell cycle G0/G1 of esophageal cancer cell strain Eca-109 cell increased obviously and cell in s period decreased significantly. This research proved that minor bupleurum decoction had anti-tumor effect and can influent proliferation and apoptosis of esophageal cancer cell strain Eca-109 cell.
authors.affiliation: Department of Gastroenterology, Henan Provincial People's Hospital, Zhengzhou, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pathology, Henan Tumor Hospital, Zhengzhou,China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Sun
authors.affiliation: Department of Pathology,the First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Pathology,the First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Pathology,the First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
authors.forename: Kuisheng
authors.initials: K
authors.lastname: Chen
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D029972:Bupleurum; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004938:Esophageal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D006801:Humans