title: Modelling the time evolution of active caspase-3 protein in the rat lens after in vivo exposure to ultraviolet radiation-B.
abstract: OBJECTIVE
  To introduce a model for the time evolution of active caspase-3 protein expression in albino rat lens up to 24 hours after in vivo exposure to low dose UVR in the 300 nm wavelength region (UVR-300 nm).
  
  
  METHODS
  Forty Sprague-Dawley rats were unilaterally exposed in vivo to 1 kJ/m2 UVR-300 nm for 15 minutes. At 0.5, 8, 16, and 24 hours after the UVR exposure, the exposed and contralateral not-exposed lenses were removed and processed for immunohistochemistry. The differences in the probability of active caspase-3 expression at four different time points after exposure were used to determine the time evolution of active caspase-3 expression. A logistic model was introduced for the expression of active caspase-3. The parameters for the exposed and the not exposed lenses were estimated for the observation time points.
  
  
  RESULTS
  The exposure to UVR-300 nm impacted on the parameters of the logistic model. Further, the parameters of the model varied with time after exposure to UVR-300 nm.
  
  
  CONCLUSIONS
  The logistic model predicts the impact of exposure to UVR-300 nm on the spatial distribution of probability of active caspase-3 protein expression, depending on time.
authors.affiliation: Gullstrand lab of Ophthalmology, Department of Neuroscience, Uppsala University, Uppsala, Sweden.
authors.forename: Nooshin
authors.initials: N
authors.lastname: Talebizadeh
authors.affiliation: Gullstrand lab of Ophthalmology, Department of Neuroscience, Uppsala University, Uppsala, Sweden.
authors.forename: Zhaohua
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Gullstrand lab of Ophthalmology, Department of Neuroscience, Uppsala University, Uppsala, Sweden.
authors.forename: Martin
authors.initials: M
authors.lastname: Kronschläger
authors.affiliation: Gullstrand lab of Ophthalmology, Department of Neuroscience, Uppsala University, Uppsala, Sweden.
authors.forename: Per
authors.initials: P
authors.lastname: Söderberg
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000378:metabolism*; D007908:Lens, Crystalline / Q000378:metabolism / Q000528:radiation effects*; D008962:Models, Theoretical; D011833:Radiation Injuries, Experimental / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D053148:Caspase 3