title: Application of FCS in studies of rhinovirus receptor binding and uncoating.
abstract: Fluorescence correlation spectroscopy (FCS) allows determining diffusion and relaxation properties of fluorescent molecules. It requires only extremely small amounts of sample, down to picomolar concentrations, in an effective analysis volume of a few femtoliters. In essence, FCS determines the autocorrelation of fluorescence fluctuations caused by single labeled molecules passing through the confocal volume of a microscope equipped with a suitable detector; it permits investigating interactions of (macro)molecules, even in single cells. We present an FCS protocol for exploring, under in vitro conditions, the dynamic processes that take place during the early steps of virus infection. We cover two important issues of rhinovirus research, the kinetics of directional RNA release, and virus-receptor interactions exemplified by using human rhinovirus type A2 (HRV-A2) as a model.
authors.affiliation: Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University of Vienna, Vienna, Austria.
authors.forename: Shushan
authors.initials: S
authors.lastname: Harutyunyan
authors.forename: Arthur
authors.initials: A
authors.lastname: Sedivy
authors.forename: Gottfried
authors.initials: G
authors.lastname: Köhler
authors.forename: Heinrich
authors.initials: H
authors.lastname: Kowalski
authors.forename: Dieter
authors.initials: D
authors.lastname: Blaas
mesh_terms: D002213:Capsid / Q000737:chemistry; D002232:Carbocyanines / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D054884:Host-Pathogen Interactions; D006801:Humans; D012367:RNA, Viral / Q000737:chemistry / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D012229:Rhinovirus / Q000378:metabolism* / Q000472:pathogenicity; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; D012367:RNA, Viral; D011991:Receptors, Virus; C089187:cyanine dye 3