title: The promoting effects of alginate oligosaccharides on root development in Oryza sativa L. mediated by auxin signaling.
abstract: Alginate oligosaccharides (AOS), which are marine oligosaccharides, are involved in regulating plant root growth, but the promotion mechanism for AOS remains unclear. Here, AOS (10-80 mg/L) induced the expression of auxin-related gene (OsYUCCA1, OsYUCCA5, OsIAA11 and OsPIN1) in rice (Oryza sativa L.) tissues to accelerate auxin biosynthesis and transport, and reduced indole-3-acetic acid (IAA) oxidase activity in rice roots. These changes resulted in the increase of 37.8% in IAA concentration in rice roots, thereby inducing the expression of root development-related genes, promoting root growth in a dose-dependent manner, which were inhibited by auxin transport inhibitor 2,3,5-triiodo benzoic acid (TIBA) and calcium-chelating agent ethylene glycol bis (2-aminoethyl) tetraacetic acid (EGTA). AOS also induced calcium signaling generation in rice roots. Those results indicated that auxin mediated AOS regulation of root development, and calcium signaling may act mainly in the upstream of auxin in the regulation of AOS on rice root development.
authors.affiliation: Institute of Plant Nutrition, Agricultural Resources and Environmental Science, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: snowgirl23@126.com.
authors.forename: Yunhong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Yin
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Wenxia
authors.initials: W
authors.lastname: Wang
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Du
authors.affiliation: Institute of Plant Nutrition, Agricultural Resources and Environmental Science, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
authors.forename: Ailing
authors.initials: A
authors.lastname: He
authors.affiliation: Institute of Plant Nutrition, Agricultural Resources and Environmental Science, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
authors.forename: Kegang
authors.initials: K
authors.lastname: Sun
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000378:metabolism; D007210:Indoleacetic Acids / Q000737:chemistry / Q000378:metabolism*; D009844:Oligosaccharides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry* / Q000254:growth & development
chemical_list: D000464:Alginates; D007210:Indoleacetic Acids; D009844:Oligosaccharides; C030737:indoleacetic acid
keywords: Alginate oligosaccharides; Auxin; Calcium signaling; Oryza sativa L.; Promoting effects; Root development