title: [Search for potential gastric cancer biomarkers using low molecular weight blood plasma proteome profiling by mass spectrometry].
abstract: Gastric cancer, one of the most widespread malignant tumors, still lacks reliable serum/plasma biomarkers of its early detection. In this study we have developed, unified, and tested a new methodology for search of gastric cancer biomarkers based on profiling of low molecular weight proteome (LMWP) (1-17 kDa). This approach included three main components: sample pre-fractionation, matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS), data analysis by a bioinformatics software package. Applicability and perspectives of the developed approach for detection of potential gastric cancer markers during LMWP analysis have been demonstrated using 69 plasma samples from patients with gastric cancer (stages I-IV) and 238 control samples. The study revealed peptides/polypeptides, which may be potentially used for detection of this pathology.
authors.forename: V E
authors.initials: VE
authors.lastname: Shevchenko
authors.forename: N E
authors.initials: NE
authors.lastname: Arnotskaia
authors.forename: E V
authors.initials: EV
authors.lastname: Ogorodnikova
authors.forename: M M
authors.initials: MM
authors.lastname: Davydov
authors.forename: M A
authors.initials: MA
authors.lastname: Ibraev
authors.forename: I N
authors.initials: IN
authors.lastname: Turkin
authors.forename: M I
authors.initials: MI
authors.lastname: Davydov
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood*; D001798:Blood Proteins / Q000378:metabolism*; D016022:Case-Control Studies; D019295:Computational Biology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000097:blood*; D009367:Neoplasm Staging; D025341:Principal Component Analysis; D020543:Proteome / Q000378:metabolism*; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013274:Stomach Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins; D009363:Neoplasm Proteins; D020543:Proteome
keywords: biomarkers; blood plasma; gastric cancer; mass spectrometry; proteome