title: Saline storage of aerobic granules and subsequent reactivation.
abstract: Loss of structural stability and bioactivity during long-term storage and operation is primary challenge to field applications of aerobic granular processes. This study for the first time stored aerobic granules in 5%w/w NaCl solution at 4°C for 187d. The stored granules were then successfully reactivated and used for 85d in sequencing batch reactors (SBR) and continuous-flow reactors (CFR) at varying levels of chemical oxygen demand (COD). High-throughput sequencing results reveal that Thauera sp., Paracoccus sp., and Nitrosomonas sp. were the predominant in the stored aerobic granules, and Pseudoxanthomonas sp. accumulated during the reactivation process. Saline storage, in which cells are in an unculturable state by saline stress, is a promising storage process for aerobic granules.
authors.affiliation: Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China; State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
authors.forename: Chunli
authors.initials: C
authors.lastname: Wan
authors.affiliation: Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China; Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan; Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan. Electronic address: djleetw@yahoo.com.tw.
authors.forename: Duu-Jong
authors.initials: DJ
authors.lastname: Lee
authors.affiliation: Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
authors.forename: Yayi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
mesh_terms: D001420:Bacteria, Aerobic / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D019149:Bioreactors / Q000382:microbiology*; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D012965:Sodium Chloride / Q000737:chemistry*
chemical_list: D012965:Sodium Chloride
keywords: Aerobic granules; High throughput sequencing; Reactivation; SOUR