title: Protein Traffic to the Plasmodium falciparum apicoplast: evidence for a sorting branch point at the Golgi.
abstract: Plasmodium falciparum, similar to many other apicomplexan parasites, contains an apicoplast, a plastid organelle of secondary endosymbiotic origin. Nuclear-encoded proteins are targeted to the apicoplast by a bipartite topogenic signal consisting of (i) an endoplasmic reticulum (ER)-type N-terminal secretory signal peptide, followed by (ii) a plant-like transit peptide. Although the signals responsible for transport of most proteins to the apicoplast are well described, the route of trafficking from the ER to the outermost apicoplast membrane is still a matter of debate. Current models of trafficking to the apicoplast suggest that proteins destined for this organelle are, on entry into the lumen of the ER, diverted from the default secretory pathway to a specialized vesicular system which carries proteins directly from the ER to the outer apicoplast membrane. Here, we have re-examined this trafficking pathway. By titrating wild-type and mutant apicoplast transit peptides against different ER retrieval sequences and studying protein transport in a brefeldin A-resistant parasite line, we generated data which suggest a direct involvement of the Golgi in traffic of soluble proteins to the P. falciparum apicoplast.
authors.affiliation: Parasitology, FB Biology, Philipps University Marburg, Karl von Frisch Straße 8, 35043 Marburg, Germany.
authors.forename: Sabrina R
authors.initials: SR
authors.lastname: Heiny
authors.forename: Sabine
authors.initials: S
authors.lastname: Pautz
authors.forename: Mario
authors.initials: M
authors.lastname: Recker
authors.forename: Jude M
authors.initials: JM
authors.lastname: Przyborski
mesh_terms: D063546:Apicoplasts / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D008954:Models, Biological; D010963:Plasmodium falciparum / Q000235:genetics / Q000378:metabolism*; D021382:Protein Sorting Signals; D021381:Protein Transport; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D055571:Secretory Pathway
chemical_list: D021382:Protein Sorting Signals; D015800:Protozoan Proteins
keywords: ER; Golgi; P. falciparum; apicoplast; malaria; protein trafficking