title: Epidermal differentiation of stem cells on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibers.
abstract: Nanomaterials with stem cells have evolved as a promising therapeutic strategy to regenerate various tissues. Tissue engineered grafts with bone marrow derived mesenchymal stem cells (BM-MSCs) can offer a cell-based therapeutic strategy for deep wounds like burns and traumatic ulcers. In this study, we have fabricated poly(3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV) nanofibers through electrospinning. The adhesion, proliferation and epidermal differentiation of BM-MSCs on PHBV nanofibers were investigated. Epidermal differentiation media containing epidermal growth factor (EGF), insulin, 3,3',5-triiodo-L-thyronine (T3), Hydrocortisone and 1α, 25-dihydroxyvitamin (D3) were used to trigger differentiation of BM-MSCs on PHBV. The proliferation of BM-MSCs on PHBV was significantly higher than the tissue culture polystyrene (TCPS) control (p < 0.05). Live/dead staining of BM-MSCs on PHBV nanofibers confirmed the change in morphology of BM-MSCs from spindle to polygonal shape indicating their differentiation into keratinocytes. The expression levels of the genes keratin (early), filaggrin (intermediate) and involucrin (late) that are involved in epidermal differentiation were upregulated in a stage-specific manner. Our results demonstrate the potential of PHBV nanofibers in promoting adhesion and differentiation of mesenchymal stem cells. This novel cellular nanofiber construct can be a better alternative to the existing therapies for skin tissue engineering.
authors.affiliation: School of Chemical & Biotechnology, Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), SASTRA University, Thanjavur, 613401, India.
authors.forename: Dhakshinamoorthy
authors.initials: D
authors.lastname: Sundaramurthi
authors.forename: Uma Maheswari
authors.initials: UM
authors.lastname: Krishnan
authors.forename: Swaminathan
authors.initials: S
authors.lastname: Sethuraman
mesh_terms: D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D000091344:Filaggrin Proteins; D015870:Gene Expression; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics; D053550:Keratin-10 / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D012867:Skin; D014945:Wound Healing; D000094122:Polyhydroxybutyrates
chemical_list: D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D053550:Keratin-10; D011091:Polyesters; D011498:Protein Precursors; C000717249:FLG protein, human; C507493:KRT10 protein, human; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C032253:involucrin