title: Blockage of Wnt/β-catenin signaling by quercetin reduces survival and proliferation of B-1 cells in vitro.
abstract: The Wnt/β-catenin signaling pathway has been shown to play an important role in controlling the proliferation, survival and differentiation of hematopoietic cells. Several Wnt/β-catenin signaling components influence hematopoietic cells fate. B-1 cells are self-renewing and spontaneously express both myeloid and lymphoid restricted transcription factors. B-1 lymphocytes play a major role in autoimmunity and are related to CD5(+) B-cell lymphomas and leukemias, such as CLL (chronic lymphocytic leukemia). Herein, we demonstrate that Wnt/β-catenin pathway is important to B-1 cell survival in vitro. The loss of Wnt signals by quercetin treatment induces a reduction in the proliferation and survival of B-1 cells. Furthermore, the quercetin treatment diminishes IL-6 production by peritoneal cells, a cytokine important to the maintenance of B-1 cells in vitro. Importantly, the IL-6 addition to B-1 cell culture prevents cells from apoptosis, even in the presence of quercetin. These data suggest that a deregulation in β-catenin signals could result in alterations in B-1 cell proliferation and differentiation. The correlation between Wnt/β-catenin and IL-6 could point out a mechanism for the expansion of B-1 cells in autoimmune disease and neoplasia.
authors.affiliation: Disciplina de Imunologia, Departmento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, UNIFESP, Brazil.
authors.forename: Marilia Campos Tavares
authors.initials: MC
authors.lastname: Novo
authors.affiliation: Disciplina de Imunologia, Departmento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, UNIFESP, Brazil.
authors.forename: Lika
authors.initials: L
authors.lastname: Osugui
authors.affiliation: Disciplina de Imunologia, Departmento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, UNIFESP, Brazil.
authors.forename: Vanessa Oliveira
authors.initials: VO
authors.lastname: dos Reis
authors.affiliation: Disciplina de Imunologia, Departmento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, UNIFESP, Brazil.
authors.forename: Ieda Maria
authors.initials: IM
authors.lastname: Longo-Maugéri
authors.affiliation: Disciplina de Imunologia, Departmento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, UNIFESP, Brazil.
authors.forename: Mario
authors.initials: M
authors.lastname: Mariano
authors.affiliation: Disciplina de Imunologia, Departmento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, UNIFESP, Brazil. Electronic address: afpopi@unifesp.br.
authors.forename: Ana Flavia
authors.initials: AF
authors.lastname: Popi
mesh_terms: D000818:Animals; D016175:B-Lymphocyte Subsets / Q000187:drug effects / Q000378:metabolism; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D015850:Interleukin-6 / Q000378:metabolism; D051379:Mice; D011794:Quercetin / Q000494:pharmacology*; D060449:Wnt Signaling Pathway / Q000187:drug effects*
chemical_list: D015850:Interleukin-6; D011794:Quercetin
keywords: B-1 cells; IL-6; Proliferation; Quercetin; Wnt; β-Catenin