title: Cloning, expression and functional analysis of the duck Toll-like receptor 5 (TLR5) gene.
abstract: Toll-like receptor 5 (TLR5) is responsible for the recognition of bacterial flagellin in vertebrates. In the present study, the first TLR5 gene in duck was cloned. The open reading frame (ORF) of duck TLR5 (dTLR5) cDNA is 2580 bp and encodes a polypeptide of 859 amino acids. We also cloned partial sequences of myeloid differentiation factor 88, 2'-5'-oligoadenylate synthetase (OAS), and myxovirus resistance (Mx) genes from duck. dTLR5 mRNA was highly expressed in the bursa of Fabricius, spleen, trachea, lung, jejunum, rectum, and skin; moderately expressed in the muscular and glandular tissues, duodenum, ileum, caecum, and pancreas; and minimally expressed in the heart, liver, kidney, and muscle. DF-1 or HeLa cells transfected with DNA constructs encoding dTLR5 can activate NF-κB leading to the activation of interleukin-6 (IL-6) promoter. When we challenged ducks with a Herts33 Newcastle disease virus (NDV), mRNA transcription of the antiviral molecules Mx, Double stranded RNA activated protein kinase (PKR), and OAS was up-regulated in the liver, lung, and spleen 1 and 2 days post-inoculation.
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai Key Laboratory of Veterinary Biotechnology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai 200240, China.
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Cheng
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Sun
authors.forename: Hengan
authors.initials: H
authors.lastname: Wang
authors.forename: Shuduan
authors.initials: S
authors.lastname: Shi
authors.forename: Yaxian
authors.initials: Y
authors.lastname: Yan
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Chan
authors.initials: C
authors.lastname: Ding
authors.forename: Jianhe
authors.initials: J
authors.lastname: Sun
mesh_terms: D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004372:Ducks; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D007113:Immunity, Innate; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000378:metabolism; D064547:Myxovirus Resistance Proteins / Q000235:genetics / Q000378:metabolism; D009521:Newcastle Disease / Q000378:metabolism; D009522:Newcastle disease virus / Q000145:classification; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D051198:Toll-Like Receptor 5 / Q000235:genetics / Q000378:metabolism*
chemical_list: D053594:Myeloid Differentiation Factor 88; D064547:Myxovirus Resistance Proteins; D012333:RNA, Messenger; D051198:Toll-Like Receptor 5; D015088:2',5'-Oligoadenylate Synthetase
keywords: Newcastle disease virus; Toll-like receptor 5; duck; innate immune response; myeloid differentiation factor 88