title: N-glycoprotein analysis discovers new up-regulated glycoproteins in colorectal cancer tissue.
abstract: Colorectal cancer is one of the leading causes of death due to cancer worldwide. Therefore, the identification of high-specificity and -sensitivity biomarkers for the early detection of colorectal cancer is urgently needed. Post-translational modifications, such as glycosylation, are known to play an important role in cancer progression. In the present work, we used a quantitative proteomic technique based on (18)O stable isotope labeling to identify differentially expressed N-linked glycoproteins in colorectal cancer tissue samples compared with healthy colorectal tissue from 19 patients undergoing colorectal cancer surgery. We identified 54 up-regulated glycoproteins in colorectal cancer samples, therefore potentially involved in the biological processes of tumorigenesis. In particular, nine of these (PLOD2, DPEP1, SE1L1, CD82, PAR1, PLOD3, S12A2, LAMP3, OLFM4) were found to be up-regulated in the great majority of the cohort, and, interestingly, the association with colorectal cancer of four (PLOD2, S12A2, PLOD3, CD82) has not been hitherto described.
authors.affiliation: Proteomics@UMG, Department of Experimental and Clinical Medicine and ‡Surgery Unit, Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro , viale Europa, 88100 Catanzaro, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Nicastri
authors.forename: Marco
authors.initials: M
authors.lastname: Gaspari
authors.forename: Rosario
authors.initials: R
authors.lastname: Sacco
authors.forename: Laura
authors.initials: L
authors.lastname: Elia
authors.forename: Caterina
authors.initials: C
authors.lastname: Gabriele
authors.forename: Roberto
authors.initials: R
authors.lastname: Romano
authors.forename: Antonia
authors.initials: A
authors.lastname: Rizzuto
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cuda
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D002853:Chromatography, Liquid; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D019295:Computational Biology; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007553:Isotope Labeling; D007558:Italy; D010103:Oxygen Isotopes; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
chemical_list: D014408:Biomarkers, Tumor; D006023:Glycoproteins; D010103:Oxygen Isotopes
keywords: 18O stable isotope labeling; N-linked glycoproteins; biomarker discovery; colorectal cancer