title: Immunohistochemical profile of stromal constituents and lymphoid cells over the course of wound healing in murine model.
abstract: OBJECTIVE
  To assess the evolution profile of the immunohistochemical expression of stromal constituents over the time-course of wound healing in a murine model.
  
  
  METHODS
  Surgical wounds were performed in the back of 24 Wistar rats. After three, seven, 14 and 21 days, six rats were euthanized and the wounded histologically processed to assess the immunohistochemical expression of CD3, CD20, CD31, α-SMA and type-I collagen. Non-injured skin samples (NSS) were used as control. Data were subjected to statistical analysis using ANOVA and Tukey test.
  
  
  RESULTS
  The mean of CD3 and CD20 positive cells in the wounds was significantly higher than in NSS at seven and 14 days (p<0.001). The blood vessels content was significantly lower than in NSS (p<0.05) at three days, but increased at seven and 14 days (p<0.01). The mean of α-SMA positive cells at seven, 14 and 21 days was higher than in NSS (p<0.05). The relative content of type I collagen increased from three to 21 days, but remained lower than in NSS (p<0.05).
  
  
  CONCLUSIONS
  Lymphoid cells, myofibroblasts and microvessels contents varied over the time-course of wound healing, with peak at seven days and progressive reduction until 21 days. The type I collagen content increased over time.
authors.affiliation: Tiradentes University, Aracaju, SE, Brazil.
authors.forename: Bernadeth Moda de
authors.initials: BM
authors.lastname: Almeida
authors.affiliation: Tiradentes University, Aracaju, SE, Brazil.
authors.forename: Marismar Fernandes do
authors.initials: MF
authors.lastname: Nascimento
authors.affiliation: UNIT, Aracaju, SE, Brazil.
authors.forename: Rose Nely
authors.initials: RN
authors.lastname: Pereira-Filho
authors.affiliation: Department of Dentistry, UNIT, Aracaju, SE, Brazil.
authors.forename: Genecy Calado de
authors.initials: GC
authors.lastname: Melo
authors.affiliation: Department of Dentistry, UNIT, Aracaju, SE, Brazil.
authors.forename: José Cleveilton dos
authors.initials: JC
authors.lastname: Santos
authors.affiliation: Department of Exact Sciences and Statistics, UNIT, Aracaju, SE, Brazil.
authors.forename: Clauberto Rodrigues de
authors.initials: CR
authors.lastname: Oliveira
authors.affiliation: Department of Medicine, UNIT, Aracaju, SE, Brazil.
authors.forename: Margarete Zanardo
authors.initials: MZ
authors.lastname: Gomes
authors.affiliation: Department of Medicine, UNIT, Aracaju, SE, Brazil.
authors.forename: Sônia Oliveira
authors.initials: SO
authors.lastname: Lima
authors.affiliation: Department of Medicine, UNIT, Aracaju, SE, Brazil.
authors.forename: Ricardo Luiz Cavalcanti de
authors.initials: RL
authors.lastname: Albuquerque-Júnior
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D018951:Antigens, CD20 / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism; D024042:Collagen Type I / Q000378:metabolism*; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000473:pathology*; D008297:Male; D058628:Myofibroblasts / Q000473:pathology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D017208:Rats, Wistar; D012867:Skin / Q000293:injuries* / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology*
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D015703:Antigens, CD; D018951:Antigens, CD20; D017252:CD3 Complex; D024042:Collagen Type I; D019408:Platelet Endothelial Cell Adhesion Molecule-1