title: A two miRNA classifier differentiates follicular thyroid carcinomas from follicular thyroid adenomas.
abstract: The inherent diagnostic limitations of thyroid fine needle aspiration (FNA), especially in the "indeterminate" category, can be partially overcome by molecular analyses. We aimed at the identification of miRNAs that could be used to improve the discrimination of indeterminate FNAs. miRNA expression profiling was performed for 17 follicular carcinomas (FTCs) and 8 follicular adenomas (FAs). The microarray results underwent cross-comparison using three additional microarray data sets. Candidate miRNAs were validated by qPCR in an independent set of 32 FTCs and 46 FAs. Sixty-eight differentially expressed miRNAs were identified. Thirteen miRNAs could be confirmed by cross comparison. A two-miRNA-classifier was established improving the diagnostic applicability and resulted in a sensitivity of 82% and a specificity of 49%. We present a classifier that has the potential to be successfully evaluated in cytology material for its capability to discriminate (mutation negative) indeterminate cytologies and thereby improving the pre-surgical diagnostics of thyroid nodules.
authors.affiliation: Institute of Automatic Control, Silesian University of Technology, ul.Akademicka 16, 44-100 Gliwice, Poland; Department of Nuclear Medicine and Endocrine Oncology, M. Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch Wybrzeze AK 15, 44-101 Gliwice, Poland; Department of Clinical Science, University of Bergen, Postboks 7800, NO-5020 Bergen, Norway.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Stokowy
authors.affiliation: Department of Nuclear Medicine and Endocrine Oncology, M. Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch Wybrzeze AK 15, 44-101 Gliwice, Poland; Laboratory of Molecular Neurobiology, Nencki Institute of Experimental Biology, Pasteura 3, Warsaw, Poland.
authors.forename: Bartosz
authors.initials: B
authors.lastname: Wojtaś
authors.affiliation: Department of Nuclear Medicine and Endocrine Oncology, M. Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch Wybrzeze AK 15, 44-101 Gliwice, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Krajewska
authors.affiliation: Department of Nuclear Medicine and Endocrine Oncology, M. Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch Wybrzeze AK 15, 44-101 Gliwice, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Stobiecka
authors.affiliation: Department of General, Visceral and Vascular Surgery, University of Halle-Wittenberg, Halle (Saale), Germany.
authors.forename: Henning
authors.initials: H
authors.lastname: Dralle
authors.affiliation: Department of General, Visceral, and Transplantation Surgery, University Medical Center of the Johannes Gutenberg University, Mainz, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Musholt
authors.affiliation: Department of Pathology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Hauptmann
authors.affiliation: Tumor Pathology Department, M. Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch Wybrzeze AK 15, 44-101 Gliwice, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Lange
authors.affiliation: Department of Endocrinology and Metabolism, Odense University Hospital, Odense, Denmark.
authors.forename: László
authors.initials: L
authors.lastname: Hegedüs
authors.affiliation: Department of Nuclear Medicine and Endocrine Oncology, M. Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch Wybrzeze AK 15, 44-101 Gliwice, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Jarząb
authors.affiliation: IZKF Leipzig, University of Leipzig, Liebigstr.21, D-04103 Leipzig, Germany.
authors.forename: Knut
authors.initials: K
authors.lastname: Krohn
authors.affiliation: Division of Endocrinology and Nephrology, University of Leipzig, Liebigstr. 21, D-04103 Leipzig, Germany. Electronic address: Ralf.Paschke@medizin.uni-leipzig.de.
authors.forename: Ralf
authors.initials: R
authors.lastname: Paschke
authors.affiliation: Division of Endocrinology and Nephrology, University of Leipzig, Liebigstr. 21, D-04103 Leipzig, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Eszlinger
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000145:classification / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism; D030541:Databases, Genetic; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D013964:Thyroid Neoplasms / Q000145:classification / Q000175:diagnosis / Q000235:genetics
chemical_list: D035683:MicroRNAs; D012334:RNA, Neoplasm
keywords: Classifier; Follicular thyroid adenoma; Follicular thyroid cancer