title: Enhanced production of ε-caprolactone by coexpression of bacterial hemoglobin gene in recombinant Escherichia coli expressing cyclohexanone monooxygenase gene.
abstract: Baeyer-Villiger (BV) oxidation of cyclohexanone to epsilon-caprolactone in a microbial system expressing cyclohexanone monooxygenase (CHMO) can be influenced by not only the efficient regeneration of NADPH but also a sufficient supply of oxygen. In this study, the bacterial hemoglobin gene from Vitreoscilla stercoraria (vhb) was introduced into the recombinant Escherichia coli expressing CHMO to investigate the effects of an oxygen-carrying protein on microbial BV oxidation of cyclohexanone. Coexpression of Vhb allowed the recombinant E. coli strain to produce a maximum epsilon-caprolactone concentration of 15.7 g/l in a fed-batch BV oxidation of cyclohexanone, which corresponded to a 43% improvement compared with the control strain expressing CHMO only under the same conditions.
authors.affiliation: Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Won-Heong
authors.initials: WH
authors.lastname: Lee
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Park
authors.forename: Myoung-Dong
authors.initials: MD
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002208:Caproates / Q000378:metabolism*; D003512:Cyclohexanones / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D007783:Lactones / Q000378:metabolism*; D060847:Metabolic Engineering; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D054793:Truncated Hemoglobins / Q000235:genetics / Q000378:metabolism*; D020589:Vitreoscilla / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D002208:Caproates; D003512:Cyclohexanones; D007783:Lactones; D011994:Recombinant Proteins; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; C121056:caprolactone; C036468:cyclohexanone; D010105:Oxygenases; C019961:cyclohexanone oxygenase