title: Environmental systems biology of cold-tolerant phenotype in Saccharomyces species adapted to grow at different temperatures.
abstract: Temperature is one of the leading factors that drive adaptation of organisms and ecosystems. Remarkably, many closely related species share the same habitat because of their different temporal or micro-spatial thermal adaptation. In this study, we seek to find the underlying molecular mechanisms of the cold-tolerant phenotype of closely related yeast species adapted to grow at different temperatures, namely S. kudriavzevii CA111 (cryo-tolerant) and S. cerevisiae 96.2 (thermo-tolerant). Using two different systems approaches, i. thermodynamic-based analysis of a genome-scale metabolic model of S. cerevisiae and ii. large-scale competition experiment of the yeast heterozygote mutant collection, genes and pathways important for the growth at low temperature were identified. In particular, defects in lipid metabolism, oxidoreductase and vitamin pathways affected yeast fitness at cold. Combining the data from both studies, a list of candidate genes was generated and mutants for two predicted cold-favouring genes, GUT2 and ADH3, were created in two natural isolates. Compared with the parental strains, these mutants showed lower fitness at cold temperatures, with S. kudriavzevii displaying the strongest defect. Strikingly, in S. kudriavzevii, these mutations also significantly improve the growth at warm temperatures. In addition, overexpression of ADH3 in S. cerevisiae increased its fitness at cold. These results suggest that temperature-induced redox imbalances could be compensated by increased glycerol accumulation or production of cytosolic acetaldehyde through the deletion of GUT2 or ADH3, respectively.
authors.affiliation: Faculty of Life Sciences, Michael Smith Building, University of Manchester, Manchester, M13 9PT, UK.
authors.forename: Caroline Mary
authors.initials: CM
authors.lastname: Paget
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Schwartz
authors.forename: Daniela
authors.initials: D
authors.lastname: Delneri
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000426:Alcohol Dehydrogenase / Q000235:genetics; D003080:Cold Temperature; D005800:Genes, Fungal; D056084:Genetic Fitness; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics; D012440:Saccharomyces / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D049490:Systems Biology; D013816:Thermodynamics
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D005993:Glycerolphosphate Dehydrogenase; C516802:ADH3 protein, S cerevisiae; D000426:Alcohol Dehydrogenase; C096203:glycerol-3-phosphate dehydrogenase (NAD(P)+)
keywords: Saccharomyces kudriavzevii; adaptation; systems biology; temperature; thermodynamics