title: Possible involvement of caspases in proliferation of neocortical neural stem/progenitor cells in the developing mouse brain.
abstract: Caspases are well-known enzymes that work as initiators and effectors of apoptosis. To elucidate the role of caspases in neurodevelopment, we sought to determine if caspases are involved in the proliferation of neural stem/progenitor cells (NPCs) in the developing mouse brain. Labeling with 5-bromo-2'-deoxyuridine (BrdU) from days 14 to 18 of pregnant mice revealed that the 18-d old fetus had many BudU-positive cells in its brain. Double-labeling revealed that active caspase-3 was co-localized with these BrdU-positive cells in the neocortex, hippocampus, and subventricular zone of the fetal brain. Active caspase-3 was detected in cultures of NPCs derived from the neocortex of 15-d old fetuses during culture periods. Importantly, the pan-caspase inhibitor z-VAD-FMK was effective at completely inhibiting neurosphere formation by the NPCs. These results suggest the possibility that the caspase cascade is essential for the proliferation of neocortical NPCs in the developing mouse brain.
authors.affiliation: Laboratory of Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University.
authors.forename: Masanori
authors.initials: M
authors.lastname: Yoneyama
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Shiba
authors.forename: Taro
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Kiyokazu
authors.initials: K
authors.lastname: Ogita
mesh_terms: D000818:Animals; D020169:Caspases / Q000032:analysis*; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D051379:Mice; D019579:Neocortex / Q000737:chemistry* / Q000196:embryology* / Q000201:enzymology; D058953:Neural Stem Cells / Q000737:chemistry* / Q000201:enzymology; D011247:Pregnancy
chemical_list: D020169:Caspases