title: Effects of human umbilical cord mesenchymal stem cells on renal ischaemia-reperfusion injury in rats.
abstract: OBJECTIVE
  This study aims to observe the function of umbilical cord-mesenchymal stem cells (UC-MSCs) labelled with enhanced green fluorescent protein (eGFP) in the repair of renal ischaemia-reperfusion (I/R) injury, to determine the effects on inflammatory cascade in an established rat model and to explore possible pathogenesis.
  
  
  METHODS
  Sixty rats were randomly divided into three groups: the sham-operated, I/R and UC-MSC treatment groups. All rats underwent right nephrectomy. Ischaemia was induced in the left kidney by occlusion of the renal artery and vein for 1hour, followed by reperfusion for 24 hours or 48 hours. Kidney samples were collected to observe morphological changes. Immunohistochemistry was performed to assess the expression of intercellular adhesion molecule 1 (ICAM-1) in the renal tissue sample, as well as the number of infiltrating polymorphonuclear neutrophils (PMNLs) and UC-MSCs with positive eGFP.
  
  
  RESULTS
  Renal histopathological damages and the expression of ICAM-1 and PMNL increased significantly in the I/R group compared with those in the sham-operated group, whereas the damages were less conspicuous in the UC-MSC treatment group.
  
  
  CONCLUSIONS
  Renal ICAM-1, which mediated PMNL infiltration and contributed to renal damage, was significantly up-regulated in the I/R group. UC-MSCs were identified to inhibit these pathological processes and protect the kidney from I/R injury.
authors.affiliation: Department of Nephrology, the First Affiliated Hospital, Liaoning Medical College, Jinzhou 121001, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Qiu
authors.affiliation: Department of Nephrology, the First Affiliated Hospital, Liaoning Medical College, Jinzhou 121001, China.
authors.forename: Dun
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Department of Nephrology, the First Affiliated Hospital, Liaoning Medical College, Jinzhou 121001, China.
authors.forename: Dongxiao
authors.initials: D
authors.lastname: Sun
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D007668:Kidney / Q000098:blood supply* / Q000473:pathology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000502:physiology; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000628:therapy*; D015203:Reproducibility of Results; D013997:Time Factors; D016896:Treatment Outcome; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: C485184:enhanced green fluorescent protein; D018799:Intercellular Adhesion Molecule-1; D049452:Green Fluorescent Proteins