title: Regulation profile of phosphatidylcholines (PCs) and lysophosphatidylcholines (LPCs) components towards UDP-glucuronosyltransferases (UGTs) isoforms.
abstract: 1.Endogenous compounds have been reported to be the regulators of UDP-glucuronosyltransferases (UGTs) isoforms. This study aims to investigate the regulatory effects of the activity of UGT isoforms by two important lipid components phosphatidylcholine (PC) and lysophosphatidylcholines (LPC) using in vitro incubation system. 2.UGTs supersomes-catalyzed 4-methylumbelliferone (4-MU) glucuronidation was used as the probe reaction to evaluate the inhibition of compounds towards UGT isoforms except UGT1A4, and UGT1A4-catalyzed trifluoperazine (TFP) glucuronidation reaction was utilized to phenotype the activity of UGT1A4. 3.About 50 μM of LPC15:0, LPC16:0, LPC17:0, LPC18:0, LPC18:1 and PC16:0, 2:0 exhibited inhibition towards more than 90% activity of UGT isoforms, and other LPC and PC components showed negligible inhibitory potential towards all the UGT isoforms. UGT1A6 and UGT1A8 were identified to be the most sensitive UGT isoforms susceptible for the inhibition by LPC15:0, LPC16:0, LPC17:0, LPC18:0, LPC18:1 and PC16:0, 2:0, indicating the strong influence of these LPC and PC components towards UGT1A6 and UGT1A8-catalyzed metabolic reaction when the concentrations of these components increased.
authors.affiliation: Department of Clinical Pharmacology, Affiliated Hospital of the Academy of Military Medical Sciences , Beijing , China .
authors.forename: Xin
authors.initials: X
authors.lastname: Gao
authors.forename: Hengyan
authors.initials: H
authors.lastname: Qu
authors.forename: Chun-Zhi
authors.initials: CZ
authors.lastname: Ai
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Cao
authors.forename: Ting
authors.initials: T
authors.lastname: Huang
authors.forename: Jian-Xing
authors.initials: JX
authors.lastname: Chen
authors.forename: Jia
authors.initials: J
authors.lastname: Zeng
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Sun
authors.forename: Mo
authors.initials: M
authors.lastname: Hong
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Gonzalez
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Liu
authors.forename: Zhong-Ze
authors.initials: ZZ
authors.lastname: Fang
mesh_terms: D055162:Biocatalysis; D020134:Catalytic Domain; D020719:Glucuronides / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008244:Lysophosphatidylcholines / Q000737:chemistry / Q000378:metabolism*; D062105:Molecular Docking Simulation; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D020719:Glucuronides; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D020033:Protein Isoforms; D011994:Recombinant Proteins; D014453:Glucuronosyltransferase
keywords: Allosteric activation; UDP-glucuronosyltransferases; lysophosphatidylcholines; phosphatidylcholine