title: Magnetic separation of proteins by a self-assembled supramolecular ternary complex.
abstract: The easy and effective separation of proteins from a mixture is crucial in proteomics. A supramolecular method is described to selectively capture and precipitate one protein from a protein mixture upon application of a magnetic field. A multivalent complex self-assembles in a dilute aqueous solution of three components: magnetic nanoparticles capped with cyclodextrin, non-covalent cross-linkers with an adamantane and a carbohydrate moiety, and lectins. The self-assembled ternary complex is precipitated in a magnetic field and readily redispersed with the aid of a non-ionic surfactant and competitive binding agents. This strategy to purify proteins by supramolecular magnetic precipitation is highly selective and efficient.
authors.affiliation: Organic Chemistry Institute and Graduate School Chemistry, Westfälische Wilhelms-Universität Münster, Correnstrasse 40, 48149 Münster (Germany).
authors.forename: Avik
authors.initials: A
authors.lastname: Samanta
authors.forename: Bart Jan
authors.initials: BJ
authors.lastname: Ravoo
mesh_terms: D002241:Carbohydrates / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry*; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D015394:Molecular Structure; D010316:Particle Size; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D040901:Proteomics; D013499:Surface Properties
chemical_list: D002241:Carbohydrates; D003432:Cross-Linking Reagents; D003505:Cyclodextrins; D037102:Lectins; D058185:Magnetite Nanoparticles; D011506:Proteins
keywords: cyclodextrins; lectins; magnetic separation; nanoparticles; self-assembly