title: Assessment of liver fibrosis: the relationship between point shear wave elastography and quantitative histological analysis.
abstract: OBJECTIVE
  Traditional pathological scoring systems for liver fibrosis progression are predominantly based on the description of architectural changes with no consideration of the amount of collagen fiber deposition. Our purpose was to explore a true histological standard in accordance with the liver stiffness measured by point shear wave elastography (PSWE) in patients with chronic hepatitis B.
  
  
  METHODS
  A total of 78 patients with liver neoplasms underwent liver stiffness measurements with PSWE as well as biochemical investigations within 3 days before partial hepatectomy. One tissue section of the liver specimens was stained with HE trichrome and evaluated traditionally with the Scheuer scoring system. The other tissue section was stained with picroSirius red and was evaluated according to the semiquantitative Chevallier et al. scoring system. In addition, this second tissue section was evaluated for the collagen proportionate area (CPA) with computer-assisted digital image analysis. The reproducibility of PSWE technology was explored through the intra-class correlation coefficient of a reliability analysis.
  
  
  RESULTS
  The PSWE technology revealed good reproducibility in liver stiffness measurements, and the PSWE values increased with the pathological severity of liver fibrosis on both the Scheuer scoring system and the semiquantitative Chevallier et al. scoring system. PSWE values exhibited more reasonable relationships with CPA (r = 0.628, P = 0.00 < 0.05) than with the Scheuer scoring system (r = 0.473, P = 0.00 < 0.05) or the Chevallier et al. semiquantitative scoring system (r = 0.487, P = 0.00 < 0.05).
  
  
  CONCLUSIONS
  CPA is a better pathological parameter than traditional semiquantitative scoring systems in accordance with liver stiffness measured by PSWE technology.
authors.affiliation: Department of Ultrasound, Zhongshan Hospital, Fudan University, Shanghai, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ding
authors.forename: Jiao-jiao
authors.initials: JJ
authors.lastname: Ma
authors.forename: Wen-ping
authors.initials: WP
authors.lastname: Wang
authors.forename: Wen-jiao
authors.initials: WJ
authors.lastname: Zeng
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Bei-jian
authors.initials: BJ
authors.lastname: Huang
authors.forename: Shi-yao
authors.initials: SY
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D003094:Collagen / Q000378:metabolism; D018450:Disease Progression; D054459:Elasticity Imaging Techniques / Q000379:methods*; D005260:Female; D019694:Hepatitis B, Chronic / Q000150:complications*; D006652:Histological Techniques / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008099:Liver / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D008103:Liver Cirrhosis / Q000175:diagnosis* / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results
chemical_list: D003094:Collagen
keywords: collagen proportionate area; liver fibrosis; liver stiffness measurement; point shear wave elastography