title: Overview of peptide and protein analysis by mass spectrometry.
abstract: Mass spectrometry is an indispensable tool for peptide and protein analysis owing to its speed, sensitivity, and versatility. It can be used to determine amino acid sequences of peptides, and to characterize a wide variety of post-translational modifications such as phosphorylation and glycosylation. Mass spectrometry can also be used to determine absolute and relative protein quantities, and can identify and quantify thousands of proteins from complex samples, which makes it an extremely powerful tool for systems biology studies. The main goals of this unit are to familiarize peptide and protein chemists and biologists with the types of mass spectrometers that are appropriate for the majority of their analytical needs, to describe the kinds of experiments that can be performed with these instruments on a routine basis, and to discuss the kinds of information that these experiments provide.
authors.affiliation: Kimmel Center for Biology and Medicine, Skirball Institute and Department of Pharmacology, New York University School of Medicine, New York, New York.
authors.forename: Guoan
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Proteomics and Biological Mass Spectrometry Laboratory, GlaxoSmithKline, King of Prussia, Pennsylvania.
authors.forename: Roland S
authors.initials: RS
authors.lastname: Annan
authors.affiliation: Broad Institute of MIT and Harvard, Cambridge, Massachusetts.
authors.forename: Steven A
authors.initials: SA
authors.lastname: Carr
authors.affiliation: Kimmel Center for Biology and Medicine, Skirball Institute and Department of Pharmacology, New York University School of Medicine, New York, New York.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Neubert
mesh_terms: D006031:Glycosylation; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000737:chemistry / Q000235:genetics; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D020539:Sequence Analysis, Protein / Q000379:methods*
chemical_list: D010455:Peptides
keywords: mass spectrometry; proteomics; review