title: Digital Image Analysis for DETCHIP®Code Determination.
abstract: DETECHIP®is a molecular sensing array used for identification of a large variety of substances. Previous methodology for the analysis of DETECHIP®used human vision to distinguish color changes induced by the presence of the analyte of interest. This paper describes several analysis techniques using digital images of DETECHIP®. Both a digital camera and flatbed desktop photo scanner were used to obtain Jpeg images. Color information within these digital images was obtained through the measurement of red-green-blue (RGB) values using software such as GIMP, Photoshop and ImageJ. Several different techniques were used to evaluate these color changes. It was determined that the flatbed scanner produced in the clearest and more reproducible images. Furthermore, codes obtained using a macro written for use within ImageJ showed improved consistency versus pervious methods.
authors.affiliation: Doane College, Department of Chemistry, 1014 Boswell Avenue, Crete, NE 68333.
authors.forename: Marcus
authors.initials: M
authors.lastname: Lyon
authors.affiliation: Doane College, Department of Chemistry, 1014 Boswell Avenue, Crete, NE 68333.
authors.forename: Mark V
authors.initials: MV
authors.lastname: Wilson
authors.affiliation: Kenyon College, Department of Chemistry, 200 N. College, Gambier, OH 43022.
authors.forename: Kerry A
authors.initials: KA
authors.lastname: Rouhier
authors.affiliation: NOVEL Chemical Solutions, 1155 E. Hwy 33, Crete, NE 68333.
authors.forename: David J
authors.initials: DJ
authors.lastname: Symonsbergen
authors.affiliation: University of Nebraska at Omaha, School of Interdisciplinary Informatics, 6001 Dodge Street, Omaha, NE 68182.
authors.forename: Kiran
authors.initials: K
authors.lastname: Bastola
authors.affiliation: University of Nebraska at Omaha, School of Interdisciplinary Informatics, 6001 Dodge Street, Omaha, NE 68182.
authors.forename: Ishwor
authors.initials: I
authors.lastname: Thapa
authors.affiliation: Doane College, Department of Chemistry, 1014 Boswell Avenue, Crete, NE 68333.
authors.forename: Andrea E
authors.initials: AE
authors.lastname: Holmes
authors.affiliation: Doane College, Department of Chemistry, 1014 Boswell Avenue, Crete, NE 68333.
authors.forename: Sharmin M
authors.initials: SM
authors.lastname: Sikich
authors.affiliation: Doane College, Department of Chemistry, 1014 Boswell Avenue, Crete, NE 68333.
authors.forename: Abby
authors.initials: A
authors.lastname: Jackson
keywords: DETECHIP®; Image Analysis; Molecular Sensing Array; Narcotics Detection; RGB Color Signal