title: Novel canine bocavirus strain associated with severe enteritis in a dog litter.
abstract: Bocaviruses are small non-enveloped viruses with a linear ssDNA genome, that belong to the genus Bocaparvovirus of the subfamiliy Parvovirinae. Bocavirus infections are associated with a wide spectrum of disease in humans and various mammalian species. Here we describe a fatal enteritis associated with infection with a novel strain of canine bocavirus 2 (CaBoV-2), that occurred in a litter of German wirehaired pointers. Necropsy performed on three puppies revealed an enteritis reminiscent of canine parvovirus associated enteritis, accompanied with signs of lymphocytolytic disease in bone marrow, spleen, lymph nodes and thymus. While other major causes of enteritis of young dogs, including canine parvovirus, were excluded, by random PCR in combination with next-generation sequencing, a novel CaBoV-2 strain was detected. Phylogenetic analysis of the genome of this novel canine bocavirus strain indicated that this virus was indeed most closely related to group 2 canine bocaviruses. Infection with canine bocavirus was confirmed by in situ hybridization, which revealed the presence of CaBoV-2 nucleic acid in the intestinal tract and lymphoid tissues of the dogs. In a small-scale retrospective analysis concerning the role of CaBoV-2 no additional cases were identified. The findings of this study provide novel insights into the pathogenicity of canine bocaviruses.
authors.affiliation: Department of Viroscience, Erasmus MC, Dr. Molewaterplein 50, 3015GE Rotterdam, The Netherlands. Electronic address: r.bodewes@erasmusmc.nl.
authors.forename: Rogier
authors.initials: R
authors.lastname: Bodewes
authors.affiliation: Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Lapp
authors.affiliation: Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Hahn
authors.affiliation: Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany.
authors.forename: André
authors.initials: A
authors.lastname: Habierski
authors.affiliation: Institute of Virology, Faculty of Veterinary Medicine, Justus-Liebig-University, Schubertstraße 81, 35392 Gießen, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Förster
authors.affiliation: Institute of Virology, Faculty of Veterinary Medicine, Justus-Liebig-University, Schubertstraße 81, 35392 Gießen, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: König
authors.affiliation: Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Wohlsein
authors.affiliation: Department of Viroscience, Erasmus MC, Dr. Molewaterplein 50, 3015GE Rotterdam, The Netherlands; Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany; Viroclinics Biosciences B.V., Marconistraat 16, 3029 AK Rotterdam, The Netherlands.
authors.forename: Albert D M E
authors.initials: AD
authors.lastname: Osterhaus
authors.affiliation: Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Baumgärtner
mesh_terms: D000818:Animals; D001483:Base Sequence; D052660:Bocavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers / Q000235:genetics; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D004751:Enteritis / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D059014:High-Throughput Nucleotide Sequencing / Q000662:veterinary; D006801:Humans; D017403:In Situ Hybridization / Q000662:veterinary; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000662:veterinary*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012189:Retrospective Studies; D013045:Species Specificity
chemical_list: D017931:DNA Primers
keywords: Bocavirus; Canine bocavirus; Dog; Enteritis; Parvovirus