title: Mass spectrometry identification of antifungal lipopeptides from Bacillus sp. BCLRB2 against Rhizoctonia solani and Sclerotinia sclerotiorum.
abstract: This work aims to characterize the bioactive molecules produced by an antagonistic Bacillus sp. strain BCLRB2 isolated from healthy leaves of olive tree against Rhizoctonia solani and Sclerotinia sclerotiorum. The bacterial strain isolated showed a high and persistent antifungal activity against the two pathogens. The free-cell supernatant showed also a high antifungal activity against R. solani and at a lower extent against S. sclerotiorum. The partial purification of the antifungal substances with methanol gradient applied to C18 column binding the Bacillus BCLRB2 culture supernatant showed that the 20% and 60% methanol fractions had a high and specific activity against S. sclerotiorum and R. solani, respectively. The mass spectrometry identification of the compounds in the fraction specifically active against S. sclerotiorum revealed the presence of bacillomycin D C16 as a major lipopeptide. The fraction specifically active against R. solani contained bacillomycin D C15 and 2 unknown lipopeptides. The 80% methanol fraction had a moderate and a broad spectrum activity against the two pathogens and consisted from two iturin D (C13 and C14) as a major lipopeptides.
authors.forename: S
authors.initials: S
authors.lastname: Elkahoui
authors.forename: N
authors.initials: N
authors.lastname: Djébali
authors.forename: I
authors.initials: I
authors.lastname: Karkouch
authors.forename: A Hadj
authors.initials: AH
authors.lastname: Ibrahim
authors.forename: L
authors.initials: L
authors.lastname: Kalai
authors.forename: S
authors.initials: S
authors.lastname: Bachkovel
authors.forename: O
authors.initials: O
authors.lastname: Tabbene
authors.forename: F
authors.initials: F
authors.lastname: Limam
mesh_terms: D000898:Antibiosis; D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides; D001407:Bacillus / Q000378:metabolism* / Q000472:pathogenicity; D061046:Biological Control Agents; D017077:Culture Media, Conditioned / Q000737:chemistry; D055666:Lipopeptides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D013058:Mass Spectrometry; D000432:Methanol; D031658:Olea / Q000382:microbiology; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012232:Rhizoctonia / Q000187:drug effects* / Q000254:growth & development; D004718:Saccharomycetales / Q000187:drug effects* / Q000254:growth & development; D012997:Solvents
chemical_list: D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D061046:Biological Control Agents; D017077:Culture Media, Conditioned; D055666:Lipopeptides; D010455:Peptides; D010456:Peptides, Cyclic; D012997:Solvents; C052532:iturin D; C027845:bacillomycin D; D000432:Methanol