title: Two new antioxidant actinosporin analogues from the calcium alginate beads culture of sponge-associated Actinokineospora sp. strain EG49.
abstract: Marine sponge-associated actinomycetes represent an exciting new resource for the identification of new and novel natural products . Previously, we have reported the isolation and structural elucidation of actinosporins A (1) and B (2) from Actinokineospora sp. strain EG49 isolated from the marine sponge Spheciospongia vagabunda. Herein, by employing different fermentation conditions on the same microorganism, we report on the isolation and antioxidant activity of structurally related metabolites, actinosporins C (3) and D (4). The antioxidant potential of actinosporins C and D was demonstrated using the ferric reducing antioxidant power (FRAP) assay. Additionally, at 1.25 μM, actinosporins C and D showed a significant antioxidant and protective capacity from the genomic damage induced by hydrogen peroxide in the human promyelocytic (HL-60) cell line.
authors.affiliation: Eskitis Institute for Drug Discovery, Griffith University, Brisbane, QLD 4111, Australia.
authors.forename: Tanja
authors.initials: T
authors.lastname: Grkovic
authors.affiliation: Department of Botany II, Julius-von-Sachs Institute for Biological Sciences, University of Würzburg, Julius-von-Sachs-Platz 3, D-97082 Würzburg, Germany.
authors.forename: Usama Ramadan
authors.initials: UR
authors.lastname: Abdelmohsen
authors.affiliation: Department of Toxicology, University of Würzburg, Würzburg, Germany.
authors.forename: Eman Maher
authors.initials: EM
authors.lastname: Othman
authors.affiliation: Department of Toxicology, University of Würzburg, Würzburg, Germany.
authors.forename: Helga
authors.initials: H
authors.lastname: Stopper
authors.affiliation: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, The John Arbuthnott Building, 161 Cathedral Street, Glasgow G4 0NR, UK.
authors.forename: RuAngelie
authors.initials: R
authors.lastname: Edrada-Ebel
authors.affiliation: Department of Botany II, Julius-von-Sachs Institute for Biological Sciences, University of Würzburg, Julius-von-Sachs-Platz 3, D-97082 Würzburg, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Hentschel
authors.affiliation: Eskitis Institute for Drug Discovery, Griffith University, Brisbane, QLD 4111, Australia. Electronic address: r.quinn@griffith.edu.au.
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Quinn
mesh_terms: D039903:Actinobacteria / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D000464:Alginates / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001551:Benz(a)Anthracenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects; D020723:Glucuronic Acid / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018922:HL-60 Cells; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D011161:Porifera / Q000382:microbiology
chemical_list: D000464:Alginates; D000975:Antioxidants; D001551:Benz(a)Anthracenes; D006027:Glycosides; D006603:Hexuronic Acids; C000592880:actinosporin A; C000599665:actinosporin C; C000599666:actinosporin D; D020723:Glucuronic Acid; D006861:Hydrogen Peroxide
keywords: Actinokineospora; Actinomycetes; Actinosporins; Antioxidant; Sponges