title: Development of a bacterial nanoparticle vaccine.
abstract: A simple procedure for obtaining protective antigens from Gram-negative bacteria and their encapsulation into immunomodulatory nanoparticles is described. A heat treatment in saline solution of whole bacteria rendered the release of small membrane vesicles containing outer membrane components and also superficial appendages, such as fractions of fimbriae and flagella. The immunogenicity of these antigens may be improved after encapsulation into poly(anhydride) nanoparticles made from the copolymer of methyl vinyl ether and maleic anhydride (Gantrez AN(®)).
authors.affiliation: Department of Microbiology, University of Navarra, C/Irunlarrea no 1, 31008, Pamplona, Spain, cgamazo@unav.es.
authors.forename: Carlos
authors.initials: C
authors.lastname: Gamazo
authors.forename: Javier
authors.initials: J
authors.lastname: Ochoa-Repáraz
authors.forename: Ibai
authors.initials: I
authors.lastname: Tamayo
authors.forename: Ana
authors.initials: A
authors.lastname: Camacho
authors.forename: Juan M
authors.initials: JM
authors.lastname: Irache
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification; D001428:Bacterial Vaccines / Q000737:chemistry* / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D006358:Hot Temperature; D008854:Microscopy, Electron; D053758:Nanoparticles; D019779:Salmonella enterica / Q000166:cytology / Q000254:growth & development / Q000276:immunology / Q000648:ultrastructure
chemical_list: D000942:Antigens, Bacterial; D001428:Bacterial Vaccines