title: Non-viral methods for generating integration-free, induced pluripotent stem cells.
abstract: Induced pluripotent stem (iPS) cells were created from mouse fibroblasts by induced expression of Yamanaka factors, Oct3/4, Sox2, Klf4, and c-Myc. This technique has quickly resulted in an exponential increase in the amount of pluripotency studies, and has provided a valuable tool in regenerative medicine. At the same time, many methodologies to generate iPS cells have been reported, and are comprised mainly of viral methods and non-viral methods. Although viral methods may not be applicable for clinical applications, various nonviral methods have been reported in recent years, including DNA vector-based approaches, transfection of mRNA, transduction of reprogramming proteins, and use of small molecule compounds. This review summarizes and evaluates these non-viral methods.
authors.forename: Xiao-Yue
authors.initials: XY
authors.lastname: Deng
authors.forename: Hu
authors.initials: H
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Xian-Tao
authors.initials: XT
authors.lastname: Fang
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Zou
authors.forename: Zhi-Ying
authors.initials: ZY
authors.lastname: Li
authors.forename: Chang-Bai
authors.initials: CB
authors.lastname: Liu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D000067470:Cellular Reprogramming Techniques; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D000090062:Kruppel-Like Factor 4; D014162:Transfection