title: Polymorphisms and haplotypes of the miR-148/152 family are associated with the risk and clinicopathological features of gastric cancer in a Northern Chinese population.
abstract: Our previous studies showed that the expressions of miR-148a, miR-152 and miR-148b are altered in gastric cancer (GC). The present study aimed to find relationship between individual single nucleotide polymorphisms (SNPs) or haplotypes of these miRNAs and susceptibility, clinicopathological parameters and prognosis of GC in a large sample of the Han population of Northern China. Twelve SNPs were genotyped using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry in a case-control study of 571 Chinese GC patients and 571 cancer-free controls. The rs11170877 G allele (P = 0.027) and the rs12231393 C allele (P = 0.034) were associated with a decreased risk of GC. However, these associations were lost after Bonferroni correction. The rs4719839 G allele was associated with Borrmann type III-IV GC (P = 0.034), increased tumour size (P = 0.020), an increased rate of lymph node metastasis (P = 0.047) and advanced TNM stage (P = 0.009). These associations were also lost after Bonferroni correction. The haplotype of miR-148b was significantly correlated with GC risk. The haplotypes in miRNA-148a were different in Borrmann types. The haplotype of miR-152 distributed various in the positive lymphovascular invasion group compared to negative group. Polymorphisms of miR-148b rs11170877 and 12231393 and their haplotypes were predictive factors of susceptibility to GC. A functional genetic variant of miRNA rs4719839 and the corresponding haplotype were associated with clinicopathological features and prognosis of advanced GCs.
authors.affiliation: Department of Surgical Oncology and General Surgery, First Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang 110001, Liaoning, China and Department of Biochemistry and Molecular Biology, China Medical University, 92 North 2nd Road, Heping District, Shenyang 110001, China.
authors.forename: Xiaowan
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Biochemistry and Molecular Biology, China Medical University, 92 North 2nd Road, Heping District, Shenyang 110001, China.
authors.forename: Guoli
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Surgical Oncology and General Surgery, First Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang 110001, Liaoning, China and Department of Biochemistry and Molecular Biology, China Medical University, 92 North 2nd Road, Heping District, Shenyang 110001, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Surgical Oncology and General Surgery, First Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang 110001, Liaoning, China and Department of Biochemistry and Molecular Biology, China Medical University, 92 North 2nd Road, Heping District, Shenyang 110001, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.affiliation: Department of Surgical Oncology and General Surgery, First Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang 110001, Liaoning, China and Department of Biochemistry and Molecular Biology, China Medical University, 92 North 2nd Road, Heping District, Shenyang 110001, China.
authors.forename: Yongxi
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Surgical Oncology and General Surgery, First Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang 110001, Liaoning, China and Department of Biochemistry and Molecular Biology, China Medical University, 92 North 2nd Road, Heping District, Shenyang 110001, China.
authors.forename: Jingxu
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Surgical Oncology and General Surgery, First Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang 110001, Liaoning, China and Department of Biochemistry and Molecular Biology, China Medical University, 92 North 2nd Road, Heping District, Shenyang 110001, China.
authors.forename: Ailin
authors.initials: A
authors.lastname: Li
authors.affiliation: Department of Surgical Oncology and General Surgery, First Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang 110001, Liaoning, China and Department of Biochemistry and Molecular Biology, China Medical University, 92 North 2nd Road, Heping District, Shenyang 110001, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Surgical Oncology and General Surgery, First Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang 110001, Liaoning, China and Department of Biochemistry and Molecular Biology, China Medical University, 92 North 2nd Road, Heping District, Shenyang 110001, China.
authors.forename: Huimian
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Surgical Oncology and General Surgery, First Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang 110001, Liaoning, China and Department of Biochemistry and Molecular Biology, China Medical University, 92 North 2nd Road, Heping District, Shenyang 110001, China josieon826@sina.cn.
authors.forename: Zhenning
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C550570:MIRN148 microRNA, human; C550856:MIRN152 microRNA, human; D035683:MicroRNAs