title: Sunflower-based biorefinery: poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production from crude glycerol, sunflower meal and levulinic acid.
abstract: Polyhydroxybutyrate (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] production was developed in bioreactor cultures using the strain Cupriavidus necator DSM 7237 cultivated on crude glycerol, sunflower meal (SFM) hydrolysates and levulinic acid as the sole fermentation feedstocks. Bacterial growth and PHB production was influenced significantly by the free amino nitrogen and inorganic phosphorus content of the SFM hydrolysate. Fed-batch bioreactor fermentations led to the production of 27gL(-1) PHB with an intracellular content of 72.9% (w/w). Continuous feeding of levulinic acid led to the production of up to 23.4gL(-1) P(3HB-co-3HV) with an intracellular content of 66.4% (w/w) and a 3HV content of 22.5mol%. A maximum 3HV content of 31mol% was achieved at earlier fermentation time (53h). Thus, levulinic acid could be combined with biodiesel industry by-products for the production of high P(3HB-co-3HV) concentration, intracellular content and industrially useful 3HV content.
authors.affiliation: Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece.
authors.forename: Vasiliki
authors.initials: V
authors.lastname: Kachrimanidou
authors.affiliation: Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece.
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Kopsahelis
authors.affiliation: Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece.
authors.forename: Seraphim
authors.initials: S
authors.lastname: Papanikolaou
authors.affiliation: Department of Chemical Engineering, University of Patras, 26504 Patras, Rio, Greece.
authors.forename: Ioannis K
authors.initials: IK
authors.lastname: Kookos
authors.affiliation: Green Chemistry Center of Excellence, University of York, Heslington, York Y010 5DD, UK.
authors.forename: Mario
authors.initials: M
authors.lastname: De Bruyn
authors.affiliation: Green Chemistry Center of Excellence, University of York, Heslington, York Y010 5DD, UK.
authors.forename: James H
authors.initials: JH
authors.lastname: Clark
authors.affiliation: Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece. Electronic address: akoutinas@aua.gr.
authors.forename: Apostolis A
authors.initials: AA
authors.lastname: Koutinas
mesh_terms: D018533:Biomass; D001704:Biopolymers / Q000096:biosynthesis; D001709:Biotechnology / Q000379:methods*; D020118:Cupriavidus necator / Q000187:drug effects / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D005990:Glycerol / Q000378:metabolism*; D006368:Helianthus / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism*; D007982:Levulinic Acids / Q000378:metabolism*; D009584:Nitrogen / Q000494:pharmacology; D011091:Polyesters / Q000378:metabolism*; D000091402:Prohibitins; D000094122:Polyhydroxybutyrates
chemical_list: D001704:Biopolymers; D005990:Glycerol; D006885:Hydroxybutyrates; D007982:Levulinic Acids; D009584:Nitrogen; D011091:Polyesters; D000091402:Prohibitins; C000717328:PHB protein, human; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C003182:poly-beta-hydroxybutyrate; C032246:levulinic acid
keywords: Bacterial bioconversion; Biodiesel industry by-products; Cupriavidus necator; Polyhydroxyalkanoates