title: Establishment and characterization of mesenchymal stem cell-like clonal stem cells from mouse salivary glands.
abstract: Successful therapy for radiation-induced salivary gland (SG) hypofunction is currently unavailable; however, tissue-specific stem cells are expected to be promising candidates for SG regeneration. Here, we present our method for the establishment of single cell-derived clonal stem cells from mouse SGs and describe their characteristics. Salivary gland-derived clonal stem cells (SGSCs) were isolated and expanded in vitro by a modified subfractionation culture method. The properties of SGSCs were examined with respect to their marker expression, gene expression, differentiation potential, and in vitro immunosuppressive activity relative to bone marrow-derived mesenchymal stem cells (BM-MSCs). SGSCs appeared to largely share the characteristics of BM-MSCs based on their marker expression, whereas they differentially expressed some genes, including AQP5, E-Cadherin, Laminin, ZO-1, and COL4. SGSCs showed the ability to differentiate into fat, bone, and cartilage cell types, as well as into α-amylase-producing and hepatocyte-like cells after appropriate induction. The in vitro immunosuppressive activity of SGSCs was found to be more potent than that of BM-MSCs. These results showed that SGSCs possess the properties of MSCs with some differential gene expression and they are salivary-specific stem cells with both epithelial and mesenchymal properties. The biological functions of SGSCs and their relevance to SG epithelial progenitor cells require further investigation.
authors.affiliation: 1 Department of Otorhinolaryngology-Head and Neck Surgery, Inha University School of Medicine , Incheon, Republic of Korea.
authors.forename: Jae-Yol
authors.initials: JY
authors.lastname: Lim
authors.forename: TacGhee
authors.initials: T
authors.lastname: Yi
authors.forename: Songyi
authors.initials: S
authors.lastname: Lee
authors.forename: Junghee
authors.initials: J
authors.lastname: Kim
authors.forename: Si-Na
authors.initials: SN
authors.lastname: Kim
authors.forename: Sun U
authors.initials: SU
authors.lastname: Song
authors.forename: Young-Mo
authors.initials: YM
authors.lastname: Kim
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D022781:Hepatocytes / Q000166:cytology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008809:Mice, Inbred C3H; D008856:Microscopy, Fluorescence; D013363:Submandibular Gland / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D000516:alpha-Amylases