title: [Synthesis and identification of artificial antigen of forsythin].
abstract: The establishment of high specificity and sensitivity method of small molecule monoclonal antibody-based immunoassay has a great importance in the study of small molecule compounds in Chinese medicine, wherein synthesis of small molecule artificial antigen is a critical step in the preparation of small molecule antibodies. Oxidation method using sodium iodide was used to synthesize immunogenic antigen (FRn-BSA) and coating antigen (FRn-OVA) of forsythin. UV spectroscopy and thin layer chromatography showed that forsythin was successfully conjugated with BSA and OVA. After immuned FRn-BSA, the mice could specifically produce anti-forsythin antibodies with titer up to 1:8 000, and the linear range was from 1 mg x L(-1) to 100 mg x L(-1). In this paper, the artificial antigen of forsythin was successfully synthesized, which can be applied for preparation of monoclonal antibodies and establishment of appropriate immune method.
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Ren
authors.forename: Hui-Hua
authors.initials: HH
authors.lastname: Qu
authors.forename: Jin-Jun
authors.initials: JJ
authors.lastname: Cheng
authors.forename: Na-Na
authors.initials: NN
authors.lastname: He
authors.forename: Sheng-Lan
authors.initials: SL
authors.lastname: Feng
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Qing-Guo
authors.initials: QG
authors.lastname: Wang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000737:chemistry / Q000276:immunology*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry / Q000276:immunology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005663:Furans / Q000737:chemistry / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000906:Antibodies; D000941:Antigens; D019086:Bridged Bicyclo Compounds, Heterocyclic; D004365:Drugs, Chinese Herbal; D005663:Furans; C423337:forsythenin