title: The human bitter taste receptor hTAS2R39 is the primary receptor for the bitterness of theaflavins.
abstract: We purified several hundred mgs of four major theaflavins (theaflavin, theaflavin-3-O-gallate, theaflavin-3'-O-gallate, and theaflavin-3,3'-O-digallate). Among the 25 hTAS2Rs expressed in HEK293T cells, hTAS2R39 and hTAS2R14 were activated by theaflavins. Both hTAS2R39 and hTAS2R14 responded to theaflavin-3'-O-gallate. In addition, hTAS2R39 was activated by theaflavin and theaflavin-3,3'-O-gallate, but not by theaflavin-3-O-gallate. In contrast, hTAS2R14 responded to theaflavin-3-O-gallate.
authors.affiliation: a Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka , Shizuoka , Japan.
authors.forename: Toyomi
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Miki
authors.initials: M
authors.lastname: Sagisaka
authors.forename: Riko
authors.initials: R
authors.lastname: Ikeda
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Nakamura
authors.forename: Noriko
authors.initials: N
authors.lastname: Matsuda
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ishii
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nakayama
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D044946:Biflavonoids / Q000378:metabolism* / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002392:Catechin / Q000378:metabolism* / Q000494:pharmacology*; D057809:HEK293 Cells; D006801:Humans; D011956:Receptors, Cell Surface / Q000378:metabolism*; D055697:Taste Perception / Q000187:drug effects*
chemical_list: D044946:Biflavonoids; D011956:Receptors, Cell Surface; C557300:TAS2R39 protein, human; C056068:theaflavin; D002392:Catechin; D002118:Calcium
keywords: human bitter taste receptor; human bitter taste receptor 14 (hTAS2R14); human bitter taste receptor 39 (hTAS2R39); theaflavins