title: Impact of long-term cryopreservation on single umbilical cord blood transplantation outcomes.
abstract: Umbilical cord blood (UCB) may be collected and cryopreserved for years before use. In vitro and murine models suggest that the duration of storage does not affect UCB progenitor cell performance; however, the impact of UCB age on clinical outcomes has not been definitely defined. This study sought to determine the effect of UCB unit cryopreservation time on hematopoietic potency. We analyzed 288 single UCB units used for transplantation from 1992 to 2013, with unit cryopreservation time ranging from .08 to 11.07 years. UCB unit post-thaw characteristics were examined, including percent recovery of total nucleated cells (TNC). The number of years the UCB unit spent in cryopreservation had no impact on TNC recovery nor UCB unit post-thaw viability. Duration of cryopreservation also had no impact on neutrophil or platelet engraftment in single UCB transplantations. These results show that UCB units can undergo cryopreservation for at least 10 years with no impact on clinical outcomes.
authors.affiliation: Pediatric Blood and Marrow Transplantation Program, University of Minnesota, Minneapolis, Minnesota.
authors.forename: Richard
authors.initials: R
authors.lastname: Mitchell
authors.affiliation: Pediatric Blood and Marrow Transplantation Program, University of Minnesota, Minneapolis, Minnesota.
authors.forename: John E
authors.initials: JE
authors.lastname: Wagner
authors.affiliation: Division of Hematology, Oncology, and Transplantation, University of Minnesota, Minneapolis, Minnesota.
authors.forename: Claudio G
authors.initials: CG
authors.lastname: Brunstein
authors.affiliation: Department of Biostatistics, University of Minnesota, Minneapolis, Minnesota.
authors.forename: Qing
authors.initials: Q
authors.lastname: Cao
authors.affiliation: Molecular and Cellular Therapeutics, University of Minnesota, Minneapolis/St Paul, Minnesota; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis/St Paul, Minnesota.
authors.forename: David H
authors.initials: DH
authors.lastname: McKenna
authors.affiliation: Pediatric Blood and Marrow Transplantation Program, University of Minnesota, Minneapolis, Minnesota.
authors.forename: Troy C
authors.initials: TC
authors.lastname: Lund
authors.affiliation: Pediatric Blood and Marrow Transplantation Program, University of Minnesota, Minneapolis, Minnesota. Electronic address: verneris@umn.edu.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Verneris
mesh_terms: D001792:Blood Platelets / Q000166:cytology / Q000502:physiology; D002452:Cell Count; D002470:Cell Survival; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods* / Q000706:statistics & numerical data; D015925:Cryopreservation / Q000379:methods*; D005312:Fetal Blood / Q000166:cytology* / Q000502:physiology; D006085:Graft Survival; D006801:Humans; D009504:Neutrophils / Q000166:cytology / Q000502:physiology; D012189:Retrospective Studies; D013997:Time Factors
keywords: Neutrophil recovery; UCB storage time; UCB transplantation; Umbilical cord blood (UCB)