title: MIP-1α enhances Jurkat cell transendothelial migration by up-regulating endothelial adhesion molecules VCAM-1 and ICAM-1.
abstract: The aim of this study is to evaluate the expression of macrophage inflammatory protein-1α (MIP-1α) in Jurkat cells and its effect on transendothelial migration. In the present study, human acute lymphoblastic leukemia Jurkat cells (Jurkat cells) were used as a model of T cells in human T-cell acute lymphoblastic leukemia (T-ALL), which demonstrated significantly higher MIP-1α expression compared with that in normal T-cell controls. The ability of Jurkat cells to cross a human brain microvascular endothelial cell (HBMEC) monolayer was almost completely abrogated by MIP-1α siRNA. In addition, the overexpression of MIP-1α resulted in the up-regulated expression of endothelial adhesion molecules, which enhanced the migration of Jurkat cells through a monolayer of HBMEC. MIP-1α levels in Jurkat cells appeared to be an important factor for its transendothelial migration, which may provide the theoretical basis to understand the mechanisms of brain metastases of T-ALL at cellular and molecular levels.
authors.affiliation: Department of Transfusion, The First Hospital of China Medical University, Shenyang, Liaoning, China. Electronic address: 13898807150@163.com.
authors.forename: Yi-Ran
authors.initials: YR
authors.lastname: Ma
authors.affiliation: Department of Cancer, The 436rd Hospital of Chinese People's Liberation Army, Shenyang, Liaoning, China.
authors.forename: Ying-Huan
authors.initials: YH
authors.lastname: Ma
mesh_terms: D001483:Base Sequence; D054405:Chemokine CCL3 / Q000235:genetics / Q000502:physiology*; D017931:DNA Primers; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D019169:Jurkat Cells; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D057705:Transendothelial and Transepithelial Migration / Q000502:physiology*; D015854:Up-Regulation; D019010:Vascular Cell Adhesion Molecule-1 / Q000502:physiology*
chemical_list: D054405:Chemokine CCL3; D017931:DNA Primers; D034741:RNA, Small Interfering; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1
keywords: Jurkat cells; MIP-1α; Transendothelial migration; VCAM-1 and ICAM-1