title: AOPPs (advanced oxidation protein products) promote apoptosis in trophoblastic cells through interference with NADPH oxidase signaling: implications for preeclampsia.
abstract: OBJECTIVE
  To investigate the role of placental advanced oxidation protein products (AOPPs) in the pathogenesis of preeclampsia (PE).
  
  
  METHODS
  Expression of AOPPs in human placental tissues collected from women with or without PE was examined by immunohistochemistry. The effect of AOPPs on in vitro trophoblast cell function was also examined. Specifically, we exposed trophoblastic cells to AOPPs and measured the production of human chorionic gonadotropin (hCG) as well as their invasion capacity using an in vitro Transwell invasion assay. We also investigated the effect of AOPPs on trophoblastic apoptosis and whether this effect could be mediated through interference in NADPH oxidase signaling.
  
  
  RESULTS
  AOPPs were expressed in placental tissues, and were significantly increased in placentas from women with PE versus normotensive controls. AOPPs also affected trophoblast cell function in vitro by significantly reducing β-HCG production and inhibiting trophoblas cell invasive capacity. Exposure to AOPPs significantly increased apoptosis in trophoblastic cells, which was mediated through the NADPH oxidase pathway.
  
  
  CONCLUSIONS
  AOPPs expression is increased in PE placentas and exposure to AOPPs adversely affects trophoblast cell function, which may contribute to the shallow trophoblast invasion that characterizes this disorder. Additional studies are needed to investigate further to determine whether AOPPs can be used as a biomarker for PE.
authors.affiliation: a The Department of Obstetrics and Gynecology , 2nd Clinical Medical College of Jinan University, Shenzhen People's Hospital , Shenzhen , P.R. China and.
authors.forename: Shuo-shi
authors.initials: SS
authors.lastname: Wang
authors.forename: Qi-tao
authors.initials: QT
authors.lastname: Huang
authors.forename: Mei
authors.initials: M
authors.lastname: Zhong
authors.forename: Qian
authors.initials: Q
authors.lastname: Yin
mesh_terms: D062107:Advanced Oxidation Protein Products / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D016022:Case-Control Studies; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism / Q000473:pathology*; D011247:Pregnancy; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014327:Trophoblasts / Q000187:drug effects* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D062107:Advanced Oxidation Protein Products; C494826:BAX protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; D019255:NADPH Oxidases
keywords: AOPPs; NADPH oxidase; apoptosis; placenta; preeclampsia; trophoblastic cells