title: Catheter-based induction of renal ischemia/reperfusion in swine: description of an experimental model.
abstract: Several techniques to induce renal ischemia have been proposed: clamp, PVA particles, and catheter-balloon. We report the development of a controlled, single-insult model of unilateral renal ischemia/reperfusion (I/R) without contralateral nephrectomy, using a suitable model, the pig. This is a balloon-catheter-based model using a percutaneous, interventional radiology procedure. One angioplasty balloon-catheter was placed into the right renal artery and inflated for 120 min and reperfusion over 24 h. Serial serums were sampled from the inferior vena cava and urine was directly sampled from the bladder throughout the experiment, and both kidneys were excised after 24 h of reperfusion. Analyses of renal structure and function were performed by hematoxylin-eosin/periodic Acid-Schiff, serum creatinine (SCr), blood urea nitrogen (BUN), fractional excretion of ions, and glucose, SDS-PAGE analysis of urinary proteins, and serum neutrophil gelatinase-associated lipocalin (NGAL). Total nitrated protein was quantified to characterize oxidative stress. Acute tubular necrosis (ATN) was identified in every animal, but only two animals showed levels of SCr above 150% of baseline values. As expected, I/R increased SCr and BUN. Fractional sodium, potassium, chloride, and bicarbonate excretion were modulated during ischemia. Serum-nitrated proteins and NGAL had two profiles: decreased with ischemia and increased after reperfusion. This decline was associated with increased protein excretion during ischemia and early reperfusion. Altogether, these data show that the renal I/R model can be performed by percutaneous approach in the swine model. This is a suitable translational model to study new early renal ischemic biomarkers and pathophysiological mechanisms in renal ischemia.
authors.affiliation: Laboratory of Genetics and Molecular Cardiology, Heart Institute, University of São Paulo Medical School, São Paulo, SP, Brazil.
authors.forename: Pamella A
authors.initials: PA
authors.lastname: Malagrino
authors.affiliation: Laboratory of Genetics and Molecular Cardiology, Heart Institute, University of São Paulo Medical School, São Paulo, SP, Brazil.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Venturini
authors.affiliation: Laboratory of Genetics and Molecular Cardiology, Heart Institute, University of São Paulo Medical School, São Paulo, SP, Brazil.
authors.forename: Patrícia S
authors.initials: PS
authors.lastname: Yogi
authors.affiliation: Laboratory of Genetics and Molecular Cardiology, Heart Institute, University of São Paulo Medical School, São Paulo, SP, Brazil.
authors.forename: Rafael
authors.initials: R
authors.lastname: Dariolli
authors.affiliation: Laboratory of Genetics and Molecular Cardiology, Heart Institute, University of São Paulo Medical School, São Paulo, SP, Brazil.
authors.forename: Kallyandra
authors.initials: K
authors.lastname: Padilha
authors.affiliation: Laboratory of Genetics and Molecular Cardiology, Heart Institute, University of São Paulo Medical School, São Paulo, SP, Brazil.
authors.forename: Bianca
authors.initials: B
authors.lastname: Kiers
authors.affiliation: Laboratory of Genetics and Molecular Cardiology, Heart Institute, University of São Paulo Medical School, São Paulo, SP, Brazil.
authors.forename: Tamiris C
authors.initials: TC
authors.lastname: Gois
authors.affiliation: Interventional Radiology Unit, Department of Radiology, Heart Institute, University of São Paulo Medical School, São Paulo, SP, Brazil.
authors.forename: Joaquim M
authors.initials: JM
authors.lastname: da Motta-Leal-Filho
authors.affiliation: Laboratory of Genetics and Molecular Cardiology, Heart Institute, University of São Paulo Medical School, São Paulo, SP, Brazil.
authors.forename: Celso K
authors.initials: CK
authors.lastname: Takimura
authors.affiliation: Laboratory of Genetics and Molecular Cardiology, Heart Institute, University of São Paulo Medical School, São Paulo, SP, Brazil.
authors.forename: Adriana C C
authors.initials: AC
authors.lastname: Girardi
authors.affiliation: Interventional Radiology Unit, Radiology Institute, Hospital das Clínicas, University of São Paulo Medical School, São Paulo, SP, Brazil.
authors.forename: Francisco C
authors.initials: FC
authors.lastname: Carnevale
authors.affiliation: Biosciences National Laboratory, LNBio, Campinas, SP, Brazil.
authors.forename: Ana C M
authors.initials: AC
authors.lastname: Zeri
authors.affiliation: Department of Pathology, University of São Paulo Medical School, São Paulo, SP, Brazil.
authors.forename: Denise M A C
authors.initials: DM
authors.lastname: Malheiros
authors.affiliation: Laboratory of Genetics and Molecular Cardiology, Heart Institute, University of São Paulo Medical School, São Paulo, SP, Brazil.
authors.forename: José E
authors.initials: JE
authors.lastname: Krieger
authors.affiliation: Laboratory of Genetics and Molecular Cardiology, Heart Institute, University of São Paulo Medical School, São Paulo, SP, Brazil.
authors.forename: Alexandre C
authors.initials: AC
authors.lastname: Pereira
keywords: Acute kidney injury; acute tubular necrosis; balloon‐catheter; ischemia; renal artery occlusion; swine model