title: Association between human leukocyte antigen-DR and demylinating Guillain-Barre syndrome.
abstract: OBJECTIVE
  To find an association between human leukocyte antigen (HLA) class II DRB1, DRB3, DRB4, and DRB5 alleles frequencies in a sample of Iraqi patients with Guillain-Barre syndrome (GBS) and compare with a healthy control group.
  
  
  METHODS
  We performed a cross-sectional study consisting of 30 Iraqi Arab patients with GBS attending the Neurological Department in the Neuroscience Hospital, Baghdad, Iraq between September 2012 and June 2013. The control group comprised 42 apparently healthy volunteers. Human leukocyte antigen genotyping for HLA DRB1, DRB3, DRB4, and DRB5 was performed using the polymerase chain reaction-sequence-specific primers method. The allele frequencies were compared across both groups. Major histocompatibility complex (MHC)-class II HLA-DR genotyping and serotyping were performed by software analysis.
  
  
  RESULTS
  We found increased frequencies of HLA genotype DRB1*03:01 (p=0.0009), DRB1*07:01 (p=0.0015), and DRB4*01:01 (p<0.0001) in patients with GBS compared with healthy controls. The HLA DR6 was increased in the control group (p<0.0001).
  
  
  CONCLUSIONS
  Our results suggest an association between HLA-DRB1*03:01, DRB1*07:01, DRB4*01:01, and HLA DR3, DR7 and a susceptibility to GBS.
authors.affiliation: HLA Typing Research Unit, Al-Kindy College of Medicine, Baghdad University, Baghdad, Iraq.
authors.forename: Zaki N
authors.initials: ZN
authors.lastname: Hasan
authors.forename: Haider H
authors.initials: HH
authors.lastname: Zalzala
authors.forename: Hyam R
authors.initials: HR
authors.lastname: Mohammedsalih
authors.forename: Batool M
authors.initials: BM
authors.lastname: Mahdi
authors.forename: Laheeb A
authors.initials: LA
authors.lastname: Abid
authors.forename: Zena N
authors.initials: ZN
authors.lastname: Shakir
authors.forename: Maithem J
authors.initials: MJ
authors.lastname: Fadhel
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D018912:Arabs / Q000235:genetics; D002648:Child; D005260:Female; D005787:Gene Frequency; D005802:Genes, MHC Class II; D020022:Genetic Predisposition to Disease; D020275:Guillain-Barre Syndrome / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology; D006684:HLA-DR Antigens / Q000032:analysis* / Q000235:genetics; D059811:HLA-DRB1 Chains / Q000032:analysis / Q000235:genetics; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D007493:Iraq / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C553502:HLA-DRB1*03:01 antigen; C487016:HLA-DRB4*01:01 antigen