title: Human eosinophils express the high affinity IgE receptor, FcεRI, in bullous pemphigoid.
abstract: Bullous pemphigoid (BP) is an autoimmune blistering disease mediated by autoantibodies targeting BP180 (type XVII collagen). Patient sera and tissues typically have IgG and IgE autoantibodies and elevated eosinophil numbers. Although the pathogenicity of the IgE autoantibodies is established in BP, their contribution to the disease process is not well understood. Our aims were two-fold: 1) To establish the clinical relationships between total and BP180-specific IgE, eosinophilia and other markers of disease activity; and 2) To determine if eosinophils from BP patients express the high affinity IgE receptor, FcεRI, as a potential mechanism of action for IgE in BP. Our analysis of 48 untreated BP patients revealed a correlation between BP180 IgG and both BP180 IgE and peripheral eosinophil count. Additionally, we established a correlation between total IgE concentration and both BP180 IgE levels and eosinophil count. When only sera from patients (n = 16) with total IgE ≥ 400 IU/ml were analyzed, BP180 IgG levels correlated with disease severity, BP230 IgG, total circulating IgE and BP180 IgE. Finally, peripheral eosinophil count correlated more strongly with levels of BP180 IgE then with BP180 IgG. Next, eosinophil FcεRI expression was investigated in the blood and skin using several methods. Peripheral eosinophils from BP patients expressed mRNA for all three chains (α, β and γ) of the FcεRI. Surface expression of the FcεRIα was confirmed on both peripheral and tissue eosinophils from most BP patients by immunostaining. Furthermore, using a proximity ligation assay, interaction of the α- and β-chains of the FcεRI was observed in some biopsy specimens, suggesting tissue expression of the trimeric receptor form in some patients. These studies provide clinical support for the relevance of IgE in BP disease and provide one mechanism of action of these antibodies, via binding to the FcεRI on eosinophils.
authors.affiliation: Department of Dermatology, University of Iowa, Iowa City, Iowa, United States of America.
authors.forename: Kelly N
authors.initials: KN
authors.lastname: Messingham
authors.affiliation: Department of Dermatology, University of Iowa, Iowa City, Iowa, United States of America.
authors.forename: Heather M
authors.initials: HM
authors.lastname: Holahan
authors.affiliation: Department of Dermatology, University of Iowa, Iowa City, Iowa, United States of America.
authors.forename: Alexandra S
authors.initials: AS
authors.lastname: Frydman
authors.affiliation: Department of Dermatology, University of Iowa, Iowa City, Iowa, United States of America.
authors.forename: Colleen
authors.initials: C
authors.lastname: Fullenkamp
authors.affiliation: Department of Dermatology, University of Iowa, Iowa City, Iowa, United States of America.
authors.forename: Rupasree
authors.initials: R
authors.lastname: Srikantha
authors.affiliation: Department of Dermatology, University of Iowa, Iowa City, Iowa, United States of America; Veterans Administration Medical Center, Iowa City, Iowa, United States of America.
authors.forename: Janet A
authors.initials: JA
authors.lastname: Fairley
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D002452:Cell Count; D015331:Cohort Studies; D004804:Eosinophils / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009928:Organ Specificity; D010391:Pemphigoid, Bullous / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017455:Receptors, IgE / Q000097:blood / Q000235:genetics*
chemical_list: D001323:Autoantibodies; D007074:Immunoglobulin G; D012333:RNA, Messenger; D017455:Receptors, IgE; D007073:Immunoglobulin E