title: Methionine restriction slows down senescence in human diploid fibroblasts.
abstract: Methionine restriction (MetR) extends lifespan in animal models including rodents. Using human diploid fibroblasts (HDF), we report here that MetR significantly extends their replicative lifespan, thereby postponing cellular senescence. MetR significantly decreased activity of mitochondrial complex IV and diminished the accumulation of reactive oxygen species. Lifespan extension was accompanied by a significant decrease in the levels of subunits of mitochondrial complex IV, but also complex I, which was due to a decreased translation rate of several mtDNA-encoded subunits. Together, these findings indicate that MetR slows down aging in human cells by modulating mitochondrial protein synthesis and respiratory chain assembly.
authors.affiliation: Institute for Biomedical Aging Research (IBA), Universität Innsbruck, Rennweg 10, 6020, Innsbruck, Austria.
authors.forename: Rafał
authors.initials: R
authors.lastname: Kozieł
authors.forename: Christoph
authors.initials: C
authors.lastname: Ruckenstuhl
authors.forename: Eva
authors.initials: E
authors.lastname: Albertini
authors.forename: Michael
authors.initials: M
authors.lastname: Neuhaus
authors.forename: Christine
authors.initials: C
authors.lastname: Netzberger
authors.forename: Maria
authors.initials: M
authors.lastname: Bust
authors.forename: Frank
authors.initials: F
authors.lastname: Madeo
authors.forename: Rudolf J
authors.initials: RJ
authors.lastname: Wiesner
authors.forename: Pidder
authors.initials: P
authors.lastname: Jansen-Dürr
mesh_terms: D000367:Age Factors; D000818:Animals; D004171:Diploidy; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D008715:Methionine / Q000008:administration & dosage / Q000172:deficiency* / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology
chemical_list: D008715:Methionine
keywords: cellular senescence; fibroblast; methionine; mitochondria; oxidative stress