title: Detection and molecular characterization of human cosavirus in a pediatric patient with acute gastroenteritis, Japan.
abstract: Human cosavirus (HCoSV) is a genus recently identified in the family Picornaviridae, which includes important pathogens in human health. The pathogenicity of HCoSV remains unclear. This study reports that an HCoSV strain, 10928/2012/JPN, was identified and collected from the stool sample of a child with acute gastroenteritis in Japan, with the detection rate of 0.16%. The patient was not co-infected with other common diarrhea-causal viruses, suggesting HCoSV as a causal pathogen in this pediatric patient. Phylogenetic and sequence analyses exhibited that the virus strain was classified as a new genotype in HCoSV A species, and this study is first to detect HCoSV in a clinical specimen collected in Japan. These results showed that surveillance of HCoSV is important for detecting viral agents in children with diarrhea, despite being the low detection rate.
authors.affiliation: Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan; Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan. Electronic address: shoko-tky@umin.ac.jp.
authors.forename: Shoko
authors.initials: S
authors.lastname: Okitsu
authors.affiliation: Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Pattara
authors.initials: P
authors.lastname: Khamrin
authors.affiliation: Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Aksara
authors.initials: A
authors.lastname: Thongprachum
authors.affiliation: Nishimura Clinic, Kyoto, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Nishimura
authors.affiliation: Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Angela F C
authors.initials: AF
authors.lastname: Kalesaran
authors.affiliation: Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Takanashi
authors.affiliation: Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Shimizu
authors.affiliation: Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hayakawa
authors.affiliation: Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Mizuguchi
authors.affiliation: Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan; Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ushijima
mesh_terms: D002675:Child, Preschool; D019143:Evolution, Molecular; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000821:virology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007564:Japan; D010802:Phylogeny; D010849:Picornaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010850:Picornaviridae Infections / Q000821:virology*; D012367:RNA, Viral / Q000032:analysis
chemical_list: D012367:RNA, Viral
keywords: Child; Diarrhea; Genotype; Human cosavirus; Japan