title: Genetic characterization of highly pathogenic H5N1 avian influenza virus from live migratory birds in Bangladesh.
abstract: Since the first outbreak of highly pathogenic avian influenza virus (HPAIV) subtype H5N1 in Bangladesh in 2007, the virus has been circulating among domestic poultry causing severe economic losses. To investigate the presence of HPAIV H5N1 in migratory birds and their potential role in virus spread, 205 pools of fecal samples from live migratory birds were analyzed. Here, the first virus isolation and genome characterization of two HPAIV H5N1 isolates from migratory birds (A/migratory bird/Bangladesh/P18/2010 and A/migratory bird/Bangladesh/P29/2010)are described. Full-length amplification, sequencing, and a comprehensive phylogenetic analysis were performed for HA, NA, M, NS, NP, PA, PB1, and PB2 gene segments. The selected migratory bird isolates belong to clade 2.3.2.1 along with recent Bangladeshi isolates from chickens, ducks, and crows which grouped in the same cluster with contemporary South and South-East Asian isolates. The studied isolates were genetically similar to other H5N1 isolates from different species within the respective clade although some unique amino acid substitutions were observed among them. Migratory birds remain a real threat for spreading pathogenic avian influenza viruses across the continent and introduction of new strains into Bangladesh.
authors.affiliation: Institute of Virology, Center for Infectious Diseases, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 29, 04103, Leipzig, Germany, mithundvm@gamil.com.
authors.forename: Rokshana
authors.initials: R
authors.lastname: Parvin
authors.forename: Abu H M
authors.initials: AH
authors.lastname: Kamal
authors.forename: Md E
authors.initials: ME
authors.lastname: Haque
authors.forename: Emdadul H
authors.initials: EH
authors.lastname: Chowdhury
authors.forename: Mohammed
authors.initials: M
authors.lastname: Giasuddin
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Islam
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Vahlenkamp
mesh_terms: D000818:Animals; D001459:Bangladesh; D001717:Birds; D016000:Cluster Analysis; D016679:Genome, Viral; D005838:Genotype; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005585:Influenza in Birds / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins