title: Effect of Hypericum perforatum L. extract on insulin resistance and lipid metabolic disorder in high-fat-diet induced obese mice.
abstract: Natural product Hypericum perforatum L. has been used in folk medicine to improve mental performance. However, the effect of H. perforatum L. on metabolism is still unknown. In order to test whether H. perforatum L. extract (EHP) has an effect on metabolic syndrome, we treated diet induced obese (DIO) C57BL/6J mice with the extract. The chemical characters of EHP were investigated with thin-layer chromatography, ultraviolet, high-performance liquid chromatography (HPLC), and HPLC-mass spectrometry fingerprint analysis. Oral glucose tolerance test (OGTT), insulin tolerance test (ITT), and the glucose infusion rate (GIR) in hyperinsulinemic-euglycemic clamp test were performed to evaluate the glucose metabolism and insulin sensitivity. Skeletal muscle was examined for lipid metabolism. The results suggest that EHP can significantly improve the glucose and lipid metabolism in DIO mice. In vitro, EHP inhibited the catalytic activity of recombinant human protein tyrosine phosphatase 1B (PTP1B) and reduced the protein and mRNA levels of PTP1B in the skeletal muscle. Moreover, expressions of genes related to fatty acid uptake and oxidation were changed by EHP in the skeletal muscle. These results suggest that EHP may improve insulin resistance and lipid metabolism in DIO mice.
authors.affiliation: Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study, Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing, 100050, China.
authors.forename: Jin-ying
authors.initials: JY
authors.lastname: Tian
authors.forename: Rong-ya
authors.initials: RY
authors.lastname: Tao
authors.forename: Xiao-lin
authors.initials: XL
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Yi-Bo
authors.initials: YB
authors.lastname: He
authors.forename: Ya-lun
authors.initials: YL
authors.lastname: Su
authors.forename: Teng-fei
authors.initials: TF
authors.lastname: Ji
authors.forename: Fei
authors.initials: F
authors.lastname: Ye
mesh_terms: D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects; D005227:Fatty Acids / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005951:Glucose Tolerance Test; D006801:Humans; D020902:Hypericum / Q000737:chemistry*; D007333:Insulin Resistance; D050356:Lipid Metabolism / Q000187:drug effects*; D008297:Male; D024821:Metabolic Syndrome / Q000188:drug therapy / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D018482:Muscle, Skeletal / Q000378:metabolism; D009765:Obesity / Q000188:drug therapy / Q000378:metabolism; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D010936:Plant Extracts; D011994:Recombinant Proteins; C516607:PTPN1 protein, human; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D005947:Glucose
keywords: Hypericum perforatum L.; PTP1B inhibitor; dyslipidemia; insulin resistance