title: Full genomic characterization of a novel genotype combination, G4P[14], of a human rotavirus strain from Barbados.
abstract: Since 2004, the Pan American Health Organization (PAHO) has carried out rotavirus surveillance in Latin America and the Caribbean. Here we report the characterization of human rotavirus with the novel G-P combination of G4P[14], detected through PAHO surveillance in Barbados. Full genome sequencing of strain RVA/Human-wt/BRB/CDC1133/2012/G4P[14] revealed that its genotype is G4-P[14]-I1-R1-C1-M1-A8-N1-T1-E1-H1. The possession of a Genogroup 1 (Wa-like) backbone distinguishes this strain from other P[14] rotavirus strains. Phylogenetic analyses suggested that this strain was likely generated by genetic reassortment between human, porcine and possibly other animal rotavirus strains and identified 7 lineages within the P[14] genotype. The results of this study reinforce the potential role of interspecies transmission in generating human rotavirus diversity through reassortment. Continued surveillance is important to determine if rotavirus vaccines will protect against strains that express the P[14] rotavirus genotype.
authors.affiliation: Gastroenteritis and Respiratory Viruses Laboratory Branch, Division of Viral Diseases, NCIRD, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Ka Ian
authors.initials: KI
authors.lastname: Tam
authors.affiliation: Gastroenteritis and Respiratory Viruses Laboratory Branch, Division of Viral Diseases, NCIRD, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Sunando
authors.initials: S
authors.lastname: Roy
authors.affiliation: Gastroenteritis and Respiratory Viruses Laboratory Branch, Division of Viral Diseases, NCIRD, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Mathew D
authors.initials: MD
authors.lastname: Esona
authors.affiliation: Public Health Laboratory, Ministry of Health, Barbados.
authors.forename: Starlene
authors.initials: S
authors.lastname: Jones
authors.affiliation: Public Health Laboratory, Ministry of Health, Barbados.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Sobers
authors.affiliation: Caribbean Public Health Agency (CARPHA), Port of Spain, Trinidad and Tobago.
authors.forename: Victoria
authors.initials: V
authors.lastname: Morris-Glasgow
authors.affiliation: Pan American Health Organization, Washington, DC, USA.
authors.forename: Gloria
authors.initials: G
authors.lastname: Rey-Benito
authors.affiliation: Gastroenteritis and Respiratory Viruses Laboratory Branch, Division of Viral Diseases, NCIRD, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Jon R
authors.initials: JR
authors.lastname: Gentsch
authors.affiliation: Gastroenteritis and Respiratory Viruses Laboratory Branch, Division of Viral Diseases, NCIRD, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: mkb6@cdc.gov.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Bowen
mesh_terms: D001460:Barbados / Q000453:epidemiology; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016865:Reassortant Viruses; D012401:Rotavirus / Q000145:classification* / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: Human; P[14]; Porcine; Reassortment; Rotavirus