title: Acidiphilium iwatense sp. nov., isolated from an acid mine drainage treatment plant, and emendation of the genus Acidiphilium.
abstract: Several strains of aerobic, acidophilic, chemo-organotrophic bacteria belonging to the genus Acidiphilium were isolated from an acid mine drainage (AMD) (pH 2.2) treatment plant. 16S rRNA gene sequence comparisons showed that most of the novel isolates formed a phylogenetically coherent group (designated Group Ia) distinguishable from any of the previously established species of the genus Acidiphilium at <98% similarity. This was supported by genomic DNA-DNA hybridization assays. The Group Ia isolates were characterized phenotypically by an oval cell morphology, non-motility, growth in the range pH 2.0-5.5 (optimum pH 3.5), lack of photosynthetic pigment and the presence of C19:0 cyclo ω8c as the main component of the cellular fatty acids and ubiquinone-10 as the major quinone. On the basis of these data, the name Acidiphilium iwatense sp. nov. is proposed to accommodate the Group Ia isolates, and the description of the genus Acidiphilium is emended. The type strain of Acidiphilium iwatense sp. nov. is MS8(T) ( =NBRC 107608(T)=KCTC 23505(T)).
authors.affiliation: Electronics-Inspired Interdisciplinary Research Institute, Toyohashi University of Technology, Tempaku-cho, Toyohashi 441-8580, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Okamura
authors.affiliation: Department of Environmental and Life Sciences, Toyohashi University of Technology, Tempaku-cho, Toyohashi 441-8580, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kawai
authors.affiliation: Faculty of Agriculture, Iwate University, Ueda, Morioka 020-8550, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Wakao
authors.affiliation: Department of Environmental and Life Sciences, Toyohashi University of Technology, Tempaku-cho, Toyohashi 441-8580, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamada
authors.affiliation: Department of Environmental and Life Sciences, Toyohashi University of Technology, Tempaku-cho, Toyohashi 441-8580, Japan.|Electronics-Inspired Interdisciplinary Research Institute, Toyohashi University of Technology, Tempaku-cho, Toyohashi 441-8580, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Hiraishi
mesh_terms: D041801:Acidiphilium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D007564:Japan; D008906:Mining; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry; D064091:Waste Disposal Facilities; D062065:Wastewater / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D062065:Wastewater; C025204:Ubiquinone Q2