title: Facile preparation of biocompatible sulfhydryl cotton fiber-based sorbents by "thiol-ene" click chemistry for biological analysis.
abstract: Sulfhydryl cotton fiber (SCF) has been widely used as adsorbent for a variety of metal ions since 1971. Thanks to the abundant thiols on SCF, in this study, we reported a universal method for the facile preparation of SCF-based materials using "thiol-ene" click chemistry for the first time. With the proposed method, two types of SCF-based materials, phenylboronic acid grafted sulfhydryl cotton fiber (SCF-PBA) and zirconium phosphonate-modified sulfhydryl cotton fiber (SCF-pVPA-Zr(4+)), were successfully prepared. The grafted functional groups onto the thiol group of SCF were demonstrated by X-ray photoelectron spectroscopy (XPS) and energy dispersive X-ray spectroscopy (EDX). The prepared fibrous materials exhibited excellent fiber strength, good stability in aqueous or nonaqueous solutions, and great biocompatibility. Moreover, we developed filter-free in-pipet-tip SPE using these SCF-based materials as adsorbent for the enrichment of ribonucleosides, glycopeptides and phosphopeptides. Our results showed that SCF-PBA adsorbent can selectively capture ribonucleosides and glycopeptides from complex biological samples. And SCF-pVPA-Zr(4+) adsorbent exhibited high selectivity and capacity in the enrichment of phosphopeptides from the digestion mixture of β-casein and bovine serum albumin (BSA), as well as human serum and nonfat milk digest. Generally, the preparation strategy can be a universal method for the synthesis of other functionalized cotton-based adsorbents with special requirement in microscale biological analysis.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University , Wuhan 430072, P.R. China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: He
authors.forename: Gang-Tian
authors.initials: GT
authors.lastname: Zhu
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Zhu
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Shao-Ting
authors.initials: ST
authors.lastname: Wang
authors.forename: Bi-Feng
authors.initials: BF
authors.lastname: Yuan
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Feng
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D001897:Boronic Acids / Q000737:chemistry; D002364:Caseins / Q000378:metabolism; D002417:Cattle; D002853:Chromatography, Liquid; D057930:Click Chemistry / Q000379:methods*; D032561:Cotton Fiber; D006020:Glycopeptides / Q000097:blood; D006801:Humans; D008892:Milk / Q000737:chemistry; D063065:Organophosphonates / Q000737:chemistry; D012263:Ribonucleosides / Q000652:urine; D044967:Serum / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism; D052616:Solid Phase Extraction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D014753:Vinyl Compounds / Q000737:chemistry; D015040:Zirconium / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D001897:Boronic Acids; D002364:Caseins; D006020:Glycopeptides; D063065:Organophosphonates; D012263:Ribonucleosides; D013438:Sulfhydryl Compounds; D014753:Vinyl Compounds; C433467:vinylphosphonic acid; D012710:Serum Albumin, Bovine; D015040:Zirconium; C010686:benzeneboronic acid
keywords: biological analysis; boronate affinity; immobilized metal ion affinity chromatography (IMAC); sulfhydryl cotton fiber (SCF); “thiol−ene” click chemistry