title: Investigation of metal sorption behavior of Slp1 from Lysinibacillus sphaericus JG-B53: a combined study using QCM-D, ICP-MS and AFM.
abstract: Surface layer proteins (S-layer) of Lysinibacillus sphaericus JG-B53 are biological compounds with several bio-based technical applications such as biosorptive materials for metal removal or rare metals recovery from the environment. Despite their well-described applications, a deeper understanding of their metal sorption behavior still remains challenging. The metal sorption ability of Au(3+), Pd(2+), Pt(2+) and Eu(3+) was investigated by ICP-MS, AFM and QCM-D which enables the sorption detection in real-time during in situ experiments. Results indicate a high binding of Pd, followed by Au, Eu and Pt to the proteins. The comparison between different methods allowed a deeper understanding of the metal sorption of isolated S-layer either frees in liquid, adsorbed forming a protein layer or as the bacteria surface.
authors.affiliation: Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf, Bautzner Landstrasse 400, 01328, Dresden, Germany, m.suhr@hzdr.de.
authors.forename: Matthias
authors.initials: M
authors.lastname: Suhr
authors.forename: Nancy
authors.initials: N
authors.lastname: Unger
authors.forename: Karen E
authors.initials: KE
authors.lastname: Viacava
authors.forename: Tobias J
authors.initials: TJ
authors.lastname: Günther
authors.forename: Johannes
authors.initials: J
authors.lastname: Raff
authors.forename: Katrin
authors.initials: K
authors.lastname: Pollmann
mesh_terms: D000327:Adsorption; D001406:Bacillaceae / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D004785:Environmental Pollutants / Q000737:chemistry; D005063:Europium / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D008562:Membrane Glycoproteins / Q000737:chemistry*; D019216:Metals, Heavy / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D010165:Palladium / Q000737:chemistry; D010984:Platinum / Q000737:chemistry; D058525:Quartz Crystal Microbalance Techniques; D016060:Sorption Detoxification; D013054:Spectrophotometry, Atomic
chemical_list: D001426:Bacterial Proteins; D004785:Environmental Pollutants; D008562:Membrane Glycoproteins; D019216:Metals, Heavy; C055764:S-layer proteins; D005063:Europium; D010984:Platinum; D010165:Palladium; D006046:Gold