title: Reproducibility of retinal nerve fiber layer thickness measures using eye tracking in children with nonglaucomatous optic neuropathy.
abstract: OBJECTIVE
  To determine the intra- and intervisit reproducibility of circumpapillary retinal nerve fiber layer (RNFL) thickness measures using eye tracking-assisted spectral-domain optical coherence tomography (SD OCT) in children with nonglaucomatous optic neuropathy.
  
  
  METHODS
  Prospective longitudinal study.
  
  
  METHODS
  Circumpapillary RNFL thickness measures were acquired with SD OCT using the eye-tracking feature at 2 separate study visits. Children with normal and abnormal vision (visual acuity ≥ 0.2 logMAR above normal and/or visual field loss) who demonstrated clinical and radiographic stability were enrolled. Intra- and intervisit reproducibility was calculated for the global average and 9 anatomic sectors by calculating the coefficient of variation and intraclass correlation coefficient.
  
  
  RESULTS
  Forty-two subjects (median age 8.6 years, range 3.9-18.2 years) met inclusion criteria and contributed 62 study eyes. Both the abnormal and normal vision cohort demonstrated the lowest intravisit coefficient of variation for the global RNFL thickness. Intervisit reproducibility remained good for those with normal and abnormal vision, although small but statistically significant increases in the coefficient of variation were observed for multiple anatomic sectors in both cohorts. The magnitude of visual acuity loss was significantly associated with the global (ß = 0.026, P < .01) and temporal sector coefficient of variation (ß = 0.099, P < .01).
  
  
  CONCLUSIONS
  SD OCT with eye tracking demonstrates highly reproducible RNFL thickness measures. Subjects with vision loss demonstrate greater intra- and intervisit variability than those with normal vision.
authors.affiliation: George Washington University School of Medicine, Children's National Health System, Washington, DC.
authors.forename: Raneem D
authors.initials: RD
authors.lastname: Rajjoub
authors.affiliation: The Gilbert Family Neurofibromatosis Institute, Children's National Health System, Washington, DC.
authors.forename: Carmelina
authors.initials: C
authors.lastname: Trimboli-Heidler
authors.affiliation: The Gilbert Family Neurofibromatosis Institute, Children's National Health System, Washington, DC; Department of Pediatrics, Children's National Health System, Washington, DC; Center for Neuroscience and Behavior, Children's National Health System, Washington, DC; The Brain Tumor Institute, Children's National Health System, Washington, DC.
authors.forename: Roger J
authors.initials: RJ
authors.lastname: Packer
authors.affiliation: The Gilbert Family Neurofibromatosis Institute, Children's National Health System, Washington, DC; Department of Ophthalmology, Children's National Health System, Washington, DC; Department of Pediatrics, Children's National Health System, Washington, DC; Center for Neuroscience and Behavior, Children's National Health System, Washington, DC. Electronic address: ravery@childrensnational.org.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Avery
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D009412:Nerve Fibers / Q000473:pathology*; D009901:Optic Nerve Diseases / Q000473:pathology*; D011446:Prospective Studies; D012044:Regression Analysis; D015203:Reproducibility of Results; D012165:Retinal Ganglion Cells / Q000473:pathology*; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods*; D014792:Visual Acuity