title: Bone mesenchymal stem cell functions on the hierarchical micro/nanotopographies of the Ti-6Al-7Nb alloy.
abstract: We investigated the response of rat bone mesenchymal stem cells (BMSC) placed on the titanium-6aluminium-7niobiuim (Ti-6Al-7Nb) alloy modified by hydrofluoric acid etch combined with subsequent anodic oxidation. Pure titanium (Ti) discs and Ti-6Al-7Nb discs were treated by hydrofluoric acid etch and anodic oxidation, and polished pure Ti discs and Ti-6Al-7Nb discs without surface modification served as controls (n=35 in each group). Scanning electron microscopy, atomic force microscopy, and radiographic photoelectron spectroscopy assays were used to detect the properties of the samples' surface. The morphology, adhesion, proliferation, and alkaline phosphatase activity of BMSC were examined using various techniques of microscopic and biological characterisation. The results showed that both Ti-6Al-7Nb samples and the pure Ti samples showed hierarchical micro/nanotopographies, and fluorine emerged on the surfaces of the samples after modification. The hierarchical micro/nanotopographies significantly increased the spreading, adhesion, and proliferation of BMSC and activity of alkaline phosphatase. In addition, modified samples of Ti-6Al-7Nb showed significantly higher alkaline phosphatase activity than modified pure Ti samples (p<0.05). The experiment successfully confirmed that Ti-6Al-7Nb alloy with hierarchical micro/nanotopographies treated by hydrofluoric acid etch and anodic oxidation possessed good biocompatibility, and may be a promising candidate for dental implants.
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, the Fourth Military Medical University, 145 West Changle Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, 145 West Changle Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Shuyin
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, 145 West Changle Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Orthodontics, School of Stomatology, the Fourth Military Medical University, 145 West Changle Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Shuning
authors.initials: S
authors.lastname: Shen
authors.affiliation: Department of Oral and Maxillofacial Surgery, the Second Hospital of Hebei Medical University, 215 West Heping Road, Shijiazhuang, Hebei 050000, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, the Fourth Military Medical University, 145 West Changle Road, Xi'an, Shaanxi 710032, PR China. Electronic address: zhaoym@fmmu.edu.cn.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, 145 West Changle Road, Xi'an, Shaanxi 710032, PR China. Electronic address: implant@fmmu.edu.cn.
authors.forename: Liang
authors.initials: L
authors.lastname: Kong
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D017554:Carbon Compounds, Inorganic / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation; D048430:Cell Shape; D003722:Dental Alloys / Q000737:chemistry*; D003764:Dental Materials / Q000737:chemistry; D003775:Dental Polishing / Q000379:methods; D006858:Hydrofluoric Acid / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry; D010084:Oxidation-Reduction; D056951:Photoelectron Spectroscopy; D051381:Rats; D017207:Rats, Sprague-Dawley; D017655:Silicon Compounds / Q000737:chemistry; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D017554:Carbon Compounds, Inorganic; D003722:Dental Alloys; D003764:Dental Materials; D017655:Silicon Compounds; C070282:Ti-6Al-7Nb alloy; D014025:Titanium; D000469:Alkaline Phosphatase; D006858:Hydrofluoric Acid; C022088:silicon carbide
keywords: Acid etch; Anodic oxidation; Bone mesenchymal stem cells; Ti-6Al-7Nb